LOCUS       DAJVQH010000001       141613 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJVQH010000001 DAJVQH010000000
VERSION     DAJVQH010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 141613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 141613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..141613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.001"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            85..1518
                     /gene="cscA"
                     /locus_tag="OQM87_000001"
     CDS             85..1518
                     /gene="cscA"
                     /locus_tag="OQM87_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCS1506297.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1526..2584)
                     /locus_tag="OQM87_000002"
     CDS             complement(1526..2584)
                     /locus_tag="OQM87_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1506298.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2752..2970
                     /locus_tag="OQM87_000003"
                     /pseudo
     CDS             2752..2970
                     /locus_tag="OQM87_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2988..4316
                     /gene="dsdA"
                     /locus_tag="OQM87_000004"
     CDS             2988..4316
                     /gene="dsdA"
                     /locus_tag="OQM87_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCS1506299.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4424..5962)
                     /gene="emrY"
                     /locus_tag="OQM87_000005"
     CDS             complement(4424..5962)
                     /gene="emrY"
                     /locus_tag="OQM87_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCS1506300.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5962..7125)
                     /gene="emrK"
                     /locus_tag="OQM87_000006"
     CDS             complement(5962..7125)
                     /gene="emrK"
                     /locus_tag="OQM87_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCS1506301.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADQISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7541..8155
                     /gene="evgA"
                     /locus_tag="OQM87_000007"
     CDS             7541..8155
                     /gene="evgA"
                     /locus_tag="OQM87_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCS1506302.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8160..11753
                     /gene="evgS"
                     /locus_tag="OQM87_000008"
     CDS             8160..11753
                     /gene="evgS"
                     /locus_tag="OQM87_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCS1506303.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11809..12954)
                     /gene="yfdE"
                     /locus_tag="OQM87_000009"
     CDS             complement(11809..12954)
                     /gene="yfdE"
                     /locus_tag="OQM87_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCS1506304.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13028..13972)
                     /gene="yfdV"
                     /locus_tag="OQM87_000010"
     CDS             complement(13028..13972)
                     /gene="yfdV"
                     /locus_tag="OQM87_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCS1506305.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFCCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14042..15736)
                     /gene="oxc"
                     /locus_tag="OQM87_000011"
     CDS             complement(14042..15736)
                     /gene="oxc"
                     /locus_tag="OQM87_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCS1506306.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15790..17040)
                     /gene="frc"
                     /locus_tag="OQM87_000012"
     CDS             complement(15790..17040)
                     /gene="frc"
                     /locus_tag="OQM87_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCS1506307.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17553..18176)
                     /locus_tag="OQM87_000013"
     CDS             complement(17553..18176)
                     /locus_tag="OQM87_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCS1506308.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18473..18748
                     /locus_tag="OQM87_000014"
     CDS             18473..18748
                     /locus_tag="OQM87_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506309.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18825..19067)
                     /locus_tag="OQM87_000015"
     CDS             complement(18825..19067)
                     /locus_tag="OQM87_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCS1506310.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19420..20340
                     /gene="lpxP"
                     /locus_tag="OQM87_000016"
     CDS             19420..20340
                     /gene="lpxP"
                     /locus_tag="OQM87_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCS1506311.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20695..20766
                     /gene="ypdK"
                     /locus_tag="OQM87_000017"
     CDS             20695..20766
                     /gene="ypdK"
                     /locus_tag="OQM87_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCS1506312.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20831..22069)
                     /gene="alaC"
                     /locus_tag="OQM87_000018"
     CDS             complement(20831..22069)
                     /gene="alaC"
                     /locus_tag="OQM87_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCS1506313.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKACVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22446..24143
                     /gene="ypdA"
                     /locus_tag="OQM87_000019"
     CDS             22446..24143
                     /gene="ypdA"
                     /locus_tag="OQM87_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCS1506314.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24158..24891
                     /gene="ypdB"
                     /locus_tag="OQM87_000020"
                     /pseudo
     CDS             24158..24891
                     /gene="ypdB"
                     /locus_tag="OQM87_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
     gene            24904..25761
                     /locus_tag="OQM87_000021"
     CDS             24904..25761
                     /locus_tag="OQM87_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1506315.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25764..28265)
                     /gene="ptsP"
                     /locus_tag="OQM87_000022"
     CDS             complement(25764..28265)
                     /gene="ptsP"
                     /locus_tag="OQM87_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1506316.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGD
                     KSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLD
                     QILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSND
                     MTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLP
                     LLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPE
                     EDVRPLLALENIFVDQDLSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTG
                     VGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVF
                     SQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28290..29327)
                     /gene="ypdE"
                     /locus_tag="OQM87_000023"
     CDS             complement(28290..29327)
                     /gene="ypdE"
                     /locus_tag="OQM87_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1506317.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29327..30410)
                     /gene="ypdF"
                     /locus_tag="OQM87_000024"
                     /pseudo
     CDS             complement(29327..30410)
                     /gene="ypdF"
                     /locus_tag="OQM87_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30425..31672)
                     /locus_tag="OQM87_000025"
     CDS             complement(30425..31672)
                     /locus_tag="OQM87_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCS1506318.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31694..32020)
                     /locus_tag="OQM87_000026"
     CDS             complement(31694..32020)
                     /locus_tag="OQM87_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCS1506319.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32239..33204)
                     /gene="glk"
                     /locus_tag="OQM87_000027"
     CDS             complement(32239..33204)
                     /gene="glk"
                     /locus_tag="OQM87_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCS1506320.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33408..34664
                     /locus_tag="OQM87_000028"
     CDS             33408..34664
                     /locus_tag="OQM87_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCS1506321.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34779..35105
                     /locus_tag="OQM87_000029"
     CDS             34779..35105
                     /locus_tag="OQM87_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1506322.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35246..36484)
                     /locus_tag="OQM87_000030"
     CDS             complement(35246..36484)
                     /locus_tag="OQM87_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCS1506323.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36820..38022
                     /gene="nupC"
                     /locus_tag="OQM87_000031"
     CDS             36820..38022
                     /gene="nupC"
                     /locus_tag="OQM87_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCS1506324.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38072..40261)
                     /locus_tag="OQM87_000032"
                     /pseudo
     CDS             complement(38072..40261)
                     /locus_tag="OQM87_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40469..40544)
                     /locus_tag="OQM87_000033"
     tRNA            complement(40469..40544)
                     /locus_tag="OQM87_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40509..40511),aa:Ala,seq:ggc)
     gene            complement(40584..40659)
                     /locus_tag="OQM87_000034"
     tRNA            complement(40584..40659)
                     /locus_tag="OQM87_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40624..40626),aa:Ala,seq:ggc)
     gene            40880..41239
                     /locus_tag="OQM87_000035"
     CDS             40880..41239
                     /locus_tag="OQM87_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1506325.1"
                     /translation="MSKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41241..41633
                     /locus_tag="OQM87_000036"
     CDS             41241..41633
                     /locus_tag="OQM87_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1506326.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41685..43100)
                     /gene="gltX"
                     /locus_tag="OQM87_000037"
     CDS             complement(41685..43100)
                     /gene="gltX"
                     /locus_tag="OQM87_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCS1506327.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43359..43434
                     /locus_tag="OQM87_000038"
     tRNA            43359..43434
                     /locus_tag="OQM87_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43392..43394,aa:Val,seq:tac)
     gene            43479..43554
                     /locus_tag="OQM87_000039"
     tRNA            43479..43554
                     /locus_tag="OQM87_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43512..43514,aa:Val,seq:tac)
     gene            43559..43634
                     /locus_tag="OQM87_000040"
     tRNA            43559..43634
                     /locus_tag="OQM87_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43592..43594,aa:Lys,seq:ttt)
     gene            43665..43790
                     /locus_tag="OQM87_000041"
     ncRNA           43665..43790
                     /ncRNA_class="other"
                     /locus_tag="OQM87_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43910..44251
                     /locus_tag="OQM87_000042"
     CDS             43910..44251
                     /locus_tag="OQM87_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCS1506328.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44242..45168)
                     /locus_tag="OQM87_000043"
     CDS             complement(44242..45168)
                     /locus_tag="OQM87_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1506329.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45258..46256
                     /locus_tag="OQM87_000044"
     CDS             45258..46256
                     /locus_tag="OQM87_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCS1506330.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46253..46471)
                     /locus_tag="OQM87_000045"
     CDS             complement(46253..46471)
                     /locus_tag="OQM87_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCS1506331.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46473..48488)
                     /gene="ligA"
                     /locus_tag="OQM87_000046"
     CDS             complement(46473..48488)
                     /gene="ligA"
                     /locus_tag="OQM87_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCS1506332.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48559..49446)
                     /gene="zipA"
                     /locus_tag="OQM87_000047"
     CDS             complement(48559..49446)
                     /gene="zipA"
                     /locus_tag="OQM87_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCS1506333.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRSMMT
                     PQKLREYQDIIREVKDANA"
     gene            49676..50437
                     /gene="cysZ"
                     /locus_tag="OQM87_000048"
     CDS             49676..50437
                     /gene="cysZ"
                     /locus_tag="OQM87_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCS1506334.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50622..51593
                     /gene="cysK"
                     /locus_tag="OQM87_000049"
     CDS             50622..51593
                     /gene="cysK"
                     /locus_tag="OQM87_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCS1506335.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51977..52234
                     /gene="ptsH"
                     /locus_tag="OQM87_000050"
     CDS             51977..52234
                     /gene="ptsH"
                     /locus_tag="OQM87_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCS1506336.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52279..54006
                     /gene="ptsI"
                     /locus_tag="OQM87_000051"
     CDS             52279..54006
                     /gene="ptsI"
                     /locus_tag="OQM87_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCS1506337.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54047..54556
                     /gene="crr"
                     /locus_tag="OQM87_000052"
     CDS             54047..54556
                     /gene="crr"
                     /locus_tag="OQM87_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCS1506338.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54598..55449)
                     /gene="pdxK"
                     /locus_tag="OQM87_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54598..55449)
                     /gene="pdxK"
                     /locus_tag="OQM87_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCS1506339.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55554..55928
                     /locus_tag="OQM87_000054"
     CDS             55554..55928
                     /locus_tag="OQM87_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCS1506340.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55961..56684
                     /locus_tag="OQM87_000055"
                     /pseudo
     CDS             55961..56684
                     /locus_tag="OQM87_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56888..57799)
                     /gene="cysM"
                     /locus_tag="OQM87_000056"
     CDS             complement(56888..57799)
                     /gene="cysM"
                     /locus_tag="OQM87_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCS1506341.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MHAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57933..59030)
                     /gene="cysA"
                     /locus_tag="OQM87_000057"
     CDS             complement(57933..59030)
                     /gene="cysA"
                     /locus_tag="OQM87_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCS1506342.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(59020..59895)
                     /gene="cysW"
                     /locus_tag="OQM87_000058"
     CDS             complement(59020..59895)
                     /gene="cysW"
                     /locus_tag="OQM87_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCS1506343.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59895..60728)
                     /gene="cysT"
                     /locus_tag="OQM87_000059"
     CDS             complement(59895..60728)
                     /gene="cysT"
                     /locus_tag="OQM87_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCS1506344.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60728..61744)
                     /gene="cysP"
                     /locus_tag="OQM87_000060"
     CDS             complement(60728..61744)
                     /gene="cysP"
                     /locus_tag="OQM87_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCS1506345.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62048..62839)
                     /gene="ucpA"
                     /locus_tag="OQM87_000061"
     CDS             complement(62048..62839)
                     /gene="ucpA"
                     /locus_tag="OQM87_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCS1506346.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(63026..63826)
                     /gene="murR"
                     /locus_tag="OQM87_000062"
     CDS             complement(63026..63826)
                     /gene="murR"
                     /locus_tag="OQM87_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCS1506347.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63990..64886
                     /gene="murQ"
                     /locus_tag="OQM87_000063"
     CDS             63990..64886
                     /gene="murQ"
                     /locus_tag="OQM87_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCS1506348.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64890..66314
                     /gene="murP"
                     /locus_tag="OQM87_000064"
     CDS             64890..66314
                     /gene="murP"
                     /locus_tag="OQM87_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCS1506349.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66494..67393)
                     /gene="yfeX"
                     /locus_tag="OQM87_000065"
     CDS             complement(66494..67393)
                     /gene="yfeX"
                     /locus_tag="OQM87_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCS1506350.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(67489..68064)
                     /locus_tag="OQM87_000066"
     CDS             complement(67489..68064)
                     /locus_tag="OQM87_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCS1506351.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68125..68574)
                     /locus_tag="OQM87_000067"
     CDS             complement(68125..68574)
                     /locus_tag="OQM87_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCS1506352.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68561..68986)
                     /locus_tag="OQM87_000068"
     CDS             complement(68561..68986)
                     /locus_tag="OQM87_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS1506353.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69200..70069
                     /gene="amiA"
                     /locus_tag="OQM87_000069"
     CDS             69200..70069
                     /gene="amiA"
                     /locus_tag="OQM87_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCS1506354.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70073..70972
                     /gene="hemF"
                     /locus_tag="OQM87_000070"
     CDS             70073..70972
                     /gene="hemF"
                     /locus_tag="OQM87_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCS1506355.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70906..72030)
                     /gene="eutR"
                     /locus_tag="OQM87_000071"
     CDS             complement(70906..72030)
                     /gene="eutR"
                     /locus_tag="OQM87_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCS1506356.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITCQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGGGSYTQSLTLINSLNAASGLPSFG"
     gene            complement(72115..72576)
                     /gene="eutK"
                     /locus_tag="OQM87_000072"
     CDS             complement(72115..72576)
                     /gene="eutK"
                     /locus_tag="OQM87_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCS1506357.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGT"
     gene            complement(72589..73248)
                     /gene="eutL"
                     /locus_tag="OQM87_000073"
     CDS             complement(72589..73248)
                     /gene="eutL"
                     /locus_tag="OQM87_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCS1506358.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(73258..74145)
                     /gene="eutC"
                     /locus_tag="OQM87_000074"
     CDS             complement(73258..74145)
                     /gene="eutC"
                     /locus_tag="OQM87_000074"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCS1506359.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(74166..75527)
                     /gene="eutB"
                     /locus_tag="OQM87_000075"
     CDS             complement(74166..75527)
                     /gene="eutB"
                     /locus_tag="OQM87_000075"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HCS1506360.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            75716..76921
                     /locus_tag="OQM87_000076"
     CDS             75716..76921
                     /locus_tag="OQM87_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1506361.1"
                     /translation="MSKTPLTAKAIDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMA
                     AGFKTVAEDWLATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPA
                     FYKALERAGSVQIQAAIRLLILTALRTAELRLCRWEWINLEDATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYADAIDSIL
                     ADGINPLEL"
     gene            77405..78175
                     /locus_tag="OQM87_000077"
     CDS             77405..78175
                     /locus_tag="OQM87_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1506362.1"
                     /translation="MRIDQILSPANEEPALANAEPAQEQEQEQEQEQEQEQEQEQEQE
                     QEQEQEQEQNKAAAIAEFKELQRQQITAQQEECRHPLAMPKPVAKANTDKPTSWFNEE
                     AEEDLRFDSKEEQLPEEREQVETEDQQNDYRNLPAVNNAMAYLFKSGLFHKLKGDELK
                     LYTWMVSKCRYRGNDKYEIWYSRTKMAKALGKSVDTIDRHLEALRKLGLIQEMRQALR
                     GKCGKTYRLRIPSKGTAEEASMTAQQHEREAKVSYLVH"
     gene            79226..79978
                     /locus_tag="OQM87_000078"
     CDS             79226..79978
                     /locus_tag="OQM87_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506363.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKQK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGYVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            80738..81133
                     /locus_tag="OQM87_000079"
     CDS             80738..81133
                     /locus_tag="OQM87_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1506364.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            81151..81372
                     /locus_tag="OQM87_000080"
     CDS             81151..81372
                     /locus_tag="OQM87_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506365.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLIC"
     gene            81437..81979
                     /locus_tag="OQM87_000081"
     CDS             81437..81979
                     /locus_tag="OQM87_000081"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506366.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            82444..82650
                     /locus_tag="OQM87_000082"
     CDS             82444..82650
                     /locus_tag="OQM87_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506367.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            82962..83447
                     /locus_tag="OQM87_000083"
     CDS             82962..83447
                     /locus_tag="OQM87_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1506368.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            83428..83706
                     /locus_tag="OQM87_000084"
     CDS             83428..83706
                     /locus_tag="OQM87_000084"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506369.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            83699..85159
                     /locus_tag="OQM87_000085"
     CDS             83699..85159
                     /locus_tag="OQM87_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1506370.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            85193..86002
                     /gene="murI"
                     /locus_tag="OQM87_000086"
     CDS             85193..86002
                     /gene="murI"
                     /locus_tag="OQM87_000086"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1506371.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(86121..86812)
                     /locus_tag="OQM87_000087"
     CDS             complement(join(86121..86566,86566..86812))
                     /locus_tag="OQM87_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCS1506372.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALPPFNIGM
                     LTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHEK
                     VIGAFIEKHMFY"
     gene            complement(87336..88709)
                     /gene="eutA"
                     /locus_tag="OQM87_000088"
     CDS             complement(87336..88709)
                     /gene="eutA"
                     /locus_tag="OQM87_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCS1506373.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(88684..89163)
                     /gene="eutP"
                     /locus_tag="OQM87_000089"
     CDS             complement(88684..89163)
                     /gene="eutP"
                     /locus_tag="OQM87_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCS1506374.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(89176..89511)
                     /gene="eutS"
                     /locus_tag="OQM87_000090"
     CDS             complement(89176..89511)
                     /gene="eutS"
                     /locus_tag="OQM87_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCS1506375.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(89804..92083)
                     /gene="maeB"
                     /locus_tag="OQM87_000091"
     CDS             complement(89804..92083)
                     /gene="maeB"
                     /locus_tag="OQM87_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCS1506376.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            92372..93322
                     /gene="tal"
                     /locus_tag="OQM87_000092"
     CDS             92372..93322
                     /gene="tal"
                     /locus_tag="OQM87_000092"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1506377.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            93342..95345
                     /gene="tkt"
                     /locus_tag="OQM87_000093"
     CDS             93342..95345
                     /gene="tkt"
                     /locus_tag="OQM87_000093"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1506378.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(95459..96490)
                     /locus_tag="OQM87_000094"
     CDS             complement(95459..96490)
                     /locus_tag="OQM87_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCS1506379.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQSFRLVRYAAEPTCDNW
                     HGPDARPTL"
     gene            complement(96616..97191)
                     /gene="nudK"
                     /locus_tag="OQM87_000095"
     CDS             complement(96616..97191)
                     /gene="nudK"
                     /locus_tag="OQM87_000095"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCS1506380.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(97259..99238)
                     /gene="aegA"
                     /locus_tag="OQM87_000096"
     CDS             complement(97259..99238)
                     /gene="aegA"
                     /locus_tag="OQM87_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCS1506381.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDTPGVYDALPFIIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            99444..101167
                     /gene="narQ"
                     /locus_tag="OQM87_000097"
                     /pseudo
     CDS             99444..101167
                     /gene="narQ"
                     /locus_tag="OQM87_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            101369..104482
                     /gene="acrD"
                     /locus_tag="OQM87_000098"
     CDS             101369..104482
                     /gene="acrD"
                     /locus_tag="OQM87_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCS1506382.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNAAINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKAEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(104581..104640)
                     /gene="ypfM"
                     /locus_tag="OQM87_000099"
     CDS             complement(104581..104640)
                     /gene="ypfM"
                     /locus_tag="OQM87_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCS1506383.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            105021..105377
                     /locus_tag="OQM87_000100"
     CDS             105021..105377
                     /locus_tag="OQM87_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCS1506384.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            105381..106508
                     /gene="dapE"
                     /locus_tag="OQM87_000101"
     CDS             105381..106508
                     /gene="dapE"
                     /locus_tag="OQM87_000101"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCS1506385.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIIEQLVA"
     gene            106536..106736
                     /locus_tag="OQM87_000102"
     CDS             106536..106736
                     /locus_tag="OQM87_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCS1506386.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(106846..107544)
                     /gene="ypfH"
                     /locus_tag="OQM87_000103"
     CDS             complement(106846..107544)
                     /gene="ypfH"
                     /locus_tag="OQM87_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1506387.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(107618..109633)
                     /gene="tmcA"
                     /locus_tag="OQM87_000104"
     CDS             complement(107618..109633)
                     /gene="tmcA"
                     /locus_tag="OQM87_000104"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCS1506388.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAL
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGCLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(109648..110511)
                     /locus_tag="OQM87_000105"
     CDS             complement(109648..110511)
                     /locus_tag="OQM87_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCS1506389.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(110679..111392)
                     /gene="purC"
                     /locus_tag="OQM87_000106"
     CDS             complement(110679..111392)
                     /gene="purC"
                     /locus_tag="OQM87_000106"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCS1506390.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(111605..112639)
                     /gene="bamC"
                     /locus_tag="OQM87_000107"
     CDS             complement(111605..112639)
                     /gene="bamC"
                     /locus_tag="OQM87_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCS1506391.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(112656..113534)
                     /gene="dapA"
                     /locus_tag="OQM87_000108"
     CDS             complement(112656..113534)
                     /gene="dapA"
                     /locus_tag="OQM87_000108"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCS1506392.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            113680..114252
                     /locus_tag="OQM87_000109"
     CDS             113680..114252
                     /locus_tag="OQM87_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCS1506393.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            114252..114722
                     /gene="bcp"
                     /locus_tag="OQM87_000110"
     CDS             114252..114722
                     /gene="bcp"
                     /locus_tag="OQM87_000110"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCS1506394.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            114975..115592
                     /gene="hyfA"
                     /locus_tag="OQM87_000111"
     CDS             114975..115592
                     /gene="hyfA"
                     /locus_tag="OQM87_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCS1506395.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            115592..117609
                     /gene="hyfB"
                     /locus_tag="OQM87_000112"
                     /pseudo
     CDS             115592..117609
                     /gene="hyfB"
                     /locus_tag="OQM87_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            117620..118561
                     /gene="hyfC"
                     /locus_tag="OQM87_000113"
     CDS             117620..118561
                     /gene="hyfC"
                     /locus_tag="OQM87_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCS1506396.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGLASALLRLHGSSG"
     gene            118589..120028
                     /gene="hyfD"
                     /locus_tag="OQM87_000114"
     CDS             118589..120028
                     /gene="hyfD"
                     /locus_tag="OQM87_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCS1506397.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            120040..120690
                     /gene="hyfE"
                     /locus_tag="OQM87_000115"
     CDS             120040..120690
                     /gene="hyfE"
                     /locus_tag="OQM87_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCS1506398.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            120695..122275
                     /gene="hyfF"
                     /locus_tag="OQM87_000116"
     CDS             120695..122275
                     /gene="hyfF"
                     /locus_tag="OQM87_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCS1506399.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFFFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            122265..123979
                     /gene="hyfG"
                     /locus_tag="OQM87_000117"
                     /pseudo
     CDS             122265..123979
                     /gene="hyfG"
                     /locus_tag="OQM87_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            123989..124534
                     /gene="hyfH"
                     /locus_tag="OQM87_000118"
     CDS             123989..124534
                     /gene="hyfH"
                     /locus_tag="OQM87_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCS1506400.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            124531..125289
                     /gene="hyfI"
                     /locus_tag="OQM87_000119"
     CDS             124531..125289
                     /gene="hyfI"
                     /locus_tag="OQM87_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCS1506401.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            125282..125695
                     /gene="hyfJ"
                     /locus_tag="OQM87_000120"
     CDS             125282..125695
                     /gene="hyfJ"
                     /locus_tag="OQM87_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCS1506402.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            125725..127737
                     /gene="hyfR"
                     /locus_tag="OQM87_000121"
                     /pseudo
     CDS             125725..127737
                     /gene="hyfR"
                     /locus_tag="OQM87_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            127759..128607
                     /locus_tag="OQM87_000122"
                     /pseudo
     CDS             127759..128607
                     /locus_tag="OQM87_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(128645..129706)
                     /locus_tag="OQM87_000123"
     CDS             complement(128645..129706)
                     /locus_tag="OQM87_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1506403.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            129919..131382
                     /gene="bepA"
                     /locus_tag="OQM87_000124"
     CDS             129919..131382
                     /gene="bepA"
                     /locus_tag="OQM87_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCS1506404.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSNLLDEWAKGNVLQQRAAQYGRALQAMKANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            131403..131762
                     /gene="arsC"
                     /locus_tag="OQM87_000125"
     CDS             131403..131762
                     /gene="arsC"
                     /locus_tag="OQM87_000125"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCS1506405.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(131895..132641)
                     /gene="hda"
                     /locus_tag="OQM87_000126"
     CDS             complement(131895..132641)
                     /gene="hda"
                     /locus_tag="OQM87_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCS1506406.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(132691..133980)
                     /gene="uraA"
                     /locus_tag="OQM87_000127"
     CDS             complement(132691..133980)
                     /gene="uraA"
                     /locus_tag="OQM87_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCS1506407.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(134066..134692)
                     /gene="upp"
                     /locus_tag="OQM87_000128"
     CDS             complement(134066..134692)
                     /gene="upp"
                     /locus_tag="OQM87_000128"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCS1506408.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            135017..136054
                     /gene="purM"
                     /locus_tag="OQM87_000129"
     CDS             135017..136054
                     /gene="purM"
                     /locus_tag="OQM87_000129"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCS1506409.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            136054..136692
                     /gene="purN"
                     /locus_tag="OQM87_000130"
     CDS             136054..136692
                     /gene="purN"
                     /locus_tag="OQM87_000130"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCS1506410.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            136864..138930
                     /gene="ppk1"
                     /locus_tag="OQM87_000131"
     CDS             136864..138930
                     /gene="ppk1"
                     /locus_tag="OQM87_000131"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCS1506411.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            138935..140476
                     /gene="ppx"
                     /locus_tag="OQM87_000132"
     CDS             138935..140476
                     /gene="ppx"
                     /locus_tag="OQM87_000132"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCS1506412.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPSTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(140515..>141613)
                     /gene="pdeF"
                     /locus_tag="OQM87_000133"
     CDS             complement(140515..>141613)
                     /gene="pdeF"
                     /locus_tag="OQM87_000133"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS1506413.1"
                     /translation="LSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDSHLK
                     QFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRCGAM
                     YLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELIGPD
                     SFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPVEVS
                     QLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASYARL
                     KNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREAVLS
                     LGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        35358 a        36499 c        34891 g        34865 t
ORIGIN      
        1 tcgcatttat gaaagtaagg gactatattt ttataaaagt taacgttaac aattcaccaa
       61 atttgcttaa ccaggatgat taaaatgacg caatctcgat tgcatgcggc gcaaaacgca
      121 ctagcaaaac ttcatgagca ccggggtaac actttctatc cccattttca cctcgcgcct
      181 cctgccggat ggatgaacga tccaaacggc ctgatctggt ttaacgatcg ttatcacgcg
      241 ttttatcaac atcacccgat gagcgaacac tggggaccaa tgcactgggg acatgccacc
      301 agtgacgata tgatccactg gcagcatgag cctattgcgc tagcgccagg agacgataat
      361 gacaaagacg ggtgtttttc aggtagtgct gtcgatgaca atggtgtcct ctcacttatc
      421 tacaccggac acgtctggct cgatggtgca ggtaatgacg atgcaattcg cgaagtacaa
      481 tgtctggcta ccagtcggga tggtattcat ttcgagaaac agggtgtgat cctcactcca
      541 ccagaaggaa tcatgcactt ccgcgatcct aaagtgtggc gtgaagccga cacatggtgg
      601 atggtagtcg gggcgaaaga tccaggcaac acggggcaga tcctgcttta tcgcggcagt
      661 tcattgcgtg aatgggcctt cgatcgcgta ctggcccacg ctgatgcggg tgaaagctat
      721 atgtgggaat gtccggactt tttcagcctt ggcgatcagc attatctgat gttttccccg
      781 cagggaatga atgccgaggg atacagttac cgaaatcgct ttcaaagtgg cgtaataccc
      841 ggaatgtggt cgccaggacg actttttgca caatccgggc attttactga acttgataac
      901 gggcatgact tttatgcacc acaaagcttt ttagcgaagg atggtcggcg tattgttatc
      961 ggctggatgg atatgtggga atcgccaatg ccctcaaaac gtgaaggatg ggcaggctgc
     1021 atgacgctgg cgcgcgagct atcagagagc aatggcaaac ttctacaacg cccggttcac
     1081 gaagctgagt cgttacgcca gcagcatcaa tctgtctctc cccgcacaat cagcaataaa
     1141 tatgttttgc aggaaaacgc gcaagcagtt gagattcagt tgcagtgggc gctgaagaac
     1201 agtgatgccg aacattacgg attacagctc ggcactggaa tgcggctgta tattgataac
     1261 caatctgagc gacttatttt gtggcggtat tacccacacg agaatttaga cggctaccgt
     1321 agtattcccc tcccgcagcg tgacacgctc gccctaagga tatttatcga tacatcatcc
     1381 gtggaagtat ttattaacga cggggaagcg gtgatgagta gtcgaatcta tccgcagcca
     1441 gaagaacggg aactgtcgct ttatgcctcc cacggagtgg ctgtgctgca acatggagca
     1501 ctctggctac tgggttaaca taatatcagg tggaacaacg gatcaacagc gggcaaggga
     1561 tccgcgtcac tcttccccct tcacgacctt caataatatg caatgcagct tcccgcccga
     1621 taatgtcatg tggaagctga attgtggtca gcggcggtaa aaacagatgc ccgacgccaa
     1681 ccagattatc aaagcccatt acggcgacat cctgcgggat acgtaccccc ttcgccaaaa
     1741 gaacctgata agccacaaag gctgcgcgat cgttaccaca tatcagaaca tcaaaatctg
     1801 gtttgcccga tttgaagtga gcattgagta aacttgcgag atcggtgtag tgatcatcac
     1861 ctgttgccat gtgaaattgt ttcacctcag ccagatctcg tccagcatca cgccaagcct
     1921 gctcaaatcc ctgccgacga taccctgttg ccaacgcact ttccggtagc cagaagcata
     1981 atggttgacg atagcccgcc gcgagcaaat gctgtgttga ttcatattgt gcagtgtaat
     2041 catcagggat ataactgggt aacgctgggt catccgccac acagttcgcc aatacaatat
     2101 tttcaccata cagagactca ggcagcgtga tatggcgcag ccccattgta gtatagataa
     2161 tgccatccgg acggtgggca agcagctgac gtgccgcgcg ggcagcgtca tcttcagaaa
     2221 aaatattgat taaaaaacta ttccagccga actcgctggc ggtttgctca atggcaagca
     2281 gaatatcaac agagaaagga gtggtagccg tgtcctgcgc cagcacggcg agagtcgacg
     2341 gcttacgtcc ttgagcgcgc atcttacggg cggaaagatc aggaacataa ttcagggtct
     2401 ggattgcctg caatacgcgg tcacgcgttg caggacgcac agattctgca ttatgcatca
     2461 cccgggagac tgtcatcatc gacactcccg ccaagcgtgc gacatccttt aatgaagcca
     2521 tacccaagcc gtttgccgta aaacgggcac tgtagcagaa acagacgtca ctggcgagat
     2581 ccaacgccct atcacctgac acagcaatac aataaaaaat aacaataatt cccggacaat
     2641 tgtccccaat tccgcctctg ttctcgccaa cgagtaacca tataggcaag taagtaaaac
     2701 atgccaactg gcatcgccaa cctggagaag aaaatataaa tatggatcat tgtgctgcca
     2761 ctgtatcccg acatcagccc ggaaattatt gcgattgcta tcggttcagg tgcaattggc
     2821 tgcacgatcg ttacggactc gcttttctgg ctggtgaagc aatattgcgg cgctacgctc
     2881 aatgaaacat ttaaatacta tacgacagcg acatttatcg cttcagtcgt cgctctggcg
     2941 ggcacattcc tgctgtcatt tatcatctaa gcgcaaagag acgtactatg gaaaacgcta
     3001 aaatgaactc gctcatcgcc cagtatccgt tggtaaagga tctggttgct cttaaagaaa
     3061 ccacctggtt taatcctggc acgacctcat tggctgaagg tttaccttat gttggcctga
     3121 ccgaacagga tgttcaggac gcccatgcgc gcttatcccg ttttgcaccc tatctggcaa
     3181 aagcatttgc tgaaactgct gccactgggg ggattattga atcagaactg gttgccattc
     3241 cggctatgca aaaacggctg gaaaaggaat atcaacaacc gatcagcggg caactcctac
     3301 tgaaaaaaga tagccatttg cccatttccg gctctataaa agcacgcggc gggatttatg
     3361 aagtcctggc acatgcagaa aaactggctc tggaagcggg gttgctgacg cttgatgatg
     3421 actacagcaa actgctttct ccggagttta aacagttctt tagccaatac agcattgctg
     3481 tgggctcaac cggaaatctg gggttatcaa tcggcattat gagcgcccgc attggcttta
     3541 aggtgacagt tcatatgtct gctgatgccc gggcatggaa aaaagcgaaa ctgcgcagcc
     3601 atggcgttac ggtcgtggaa tatgagcaag attatggtgt tgccgtcgag gaaggacgta
     3661 aagcagcgca gtctgacccg aactgtttct ttattgatga cgaaaattcc cgcacgttgt
     3721 tccttgggta ttccgtcgct ggccagcgtc ttaaagcgca atttgcccag caaggccgta
     3781 tcgtcgatgc tgataaccct ctgtttgtct atctgccgtg tggtgttggc ggtggtcctg
     3841 gtggcgtcgc attcgggctt aaactggcgt ttggcgatca tgttcactgc ttttttgccg
     3901 aaccaacgca ctccccttgt atgttgttag gcgtccatac aggattacac gatcagattt
     3961 ctgttcagga tattggtatc gacaacctta ccgcagcgga tggccttgca gttggtcgcg
     4021 catcaggctt tgtcgggcgg gcaatggagc gtctgctgga tggcttctat acccttagcg
     4081 atcaaaccat gtatgacatg cttggctggc tggcgcagga agaaggtatt cgtcttgaac
     4141 cttcagcact ggcgggtatg gccggacctc agcgcgtgtg tgcatcagta agttaccaac
     4201 agatgcacgg tttcagcgca gaacaattgc gtaatgccac tcatctggtg tgggcgacgg
     4261 gaggtggaat ggtgccggaa gaagagatga atcaatatct ggcaaaaggc cgttaataac
     4321 gtttcaacgc agcatcgcaa tcctttccct gggtgagcga tgctgccgat ggcgcagact
     4381 taagatcccc ggtcttaccc gctataaccc cctcctttta tcatcaccca acgcctttcg
     4441 ctgtaaacgg cggtttcgca aaccaaacca aaaccgtaag taagataaaa gcaatagccg
     4501 ccatgcggaa aatctcattt gcagaaatag aaagtgactg ttgggttatt tcattattaa
     4561 tttcattaag aactcctgaa agcgaaccat agtatttatc cataatttgt gacgatgaat
     4621 taaacacggg gttaaattga tcgatggttg ctgtcaactg actatggtgt aacgattcgc
     4681 gtcgtcccca cagcgtcatt gtcaacgacg taccaactga tcctgacaag gtacgaaaaa
     4741 aattactcat actcgaggca ttggcaaatt tattatctgg caagcctgaa aacgaaatcg
     4801 ttgttaaggg taaaaagaaa caggcaacgg cgaatccctg gaaaaactgt ggcaaaatga
     4861 tgcctgtaaa atcaatcgtt ggcataaatg tcacagaacg ccagtaatag caaaccgcat
     4921 acatcaaaaa actgaatgtc actaacaccc gcatgtctat tttattgcca taacgtccta
     4981 tcaaaggtga aatcaatagt ggcatgatgc cgatgggcgc ataagcaagc ccggcccata
     5041 tcgcattata ccccatcgtt ttctggagta actgcggcat aaggacgatc gctccagagt
     5101 aaaataaata cgcgcaggtg atactcacaa taccaatggt gaaattacgg gacttaaaca
     5161 aactgagatc aagaatcggg ttctctgagg tcgattccca aatgactaaa gagatcagag
     5221 aaataactga tactactgtt aatattatta ttgtactcga gttgaaccaa tccagatcgc
     5281 gccctttatc aagcataatt tgcaagccac caacaccgag cactaacagg gtcagtcttg
     5341 gtagattcat ttttaccggt gaagtttcag tttctcttcc tttaagtaag gttaagcata
     5401 atgtcaggac gataatcccc atagggacat tgattaaaaa tatccaaccc cagctaaagt
     5461 tatcgcaaat atagccgccc aatatcggcc cacatatcgg agcgataatc acggtcattg
     5521 accataatgc cagagcaaat gttctttttt ctggtggata attccttaat aataaactct
     5581 gtgacagtgg aattaacggc cccgccatta atccctgaac gactctaaaa aatatcagca
     5641 catcaagatt ggtcgataaa ctacacatta aggaagacag cgaaaaaaaa gtgactgaaa
     5701 gtaaaaataa ccttaattcg cctattcttt gtgccagcct gccggtaaca gggatcgcaa
     5761 tggcatttgc tacgccaaac gaggtgataa cccaggtgcc ttcgtctgtt gatgctccca
     5821 gaaagccaga tattgtcggt attgcgacgt tagaaatagt ggaatccaac atttgcataa
     5881 acgtcgctaa tgacaatgca atagtaacgc accataacgt cccaccggtt aatggtgccg
     5941 gagttgattt agtgattgcc attaaagttg tccattatgc gaaataatgt tgctaatttc
     6001 tttttctatc ggactggtat cgataactaa agccttactg gtataagcag gcatggaggt
     6061 cacggttgaa gccagatcag gcatctcagc aatgtcttcg tccttcgtat caatagttgc
     6121 agtcatcgat aaaccaatgc gcaaggggtg ttccatgagt tcttttggat caagagaaac
     6181 ttcaaccggt acacgctgaa cgattttgat ccagttccct gtcgcatttt gtgcaggtaa
     6241 taaggagaac gcattgccgg ttcccatatt gatccctgtc acccgaccat gaaacacaac
     6301 attttcacca taaagatcgc tgataatatt gactgattga ccaatccgta catccgtgag
     6361 ttgtgtttct ttaaagttgg cattaaccca catttgacgt gccggtacga cagccattaa
     6421 cgattgtccg gaactcactg tttcgccgac ctgaacactt cgctgggcaa tatagccagt
     6481 aaccggactc cttatatccg tacgtttaag caccaaccag gcttctttgg ttgcatccgc
     6541 cgcttcaact acttgtggct gacggtttaa tggtgtgttc attactaaag ctttattcgc
     6601 tttataagcc tggatagcgg cattcagtgc cgctttgcta cttattaacg tatctttggt
     6661 atgctccagc gtttcttttg aaataacccc ctgcttcgct aaaggcacac gacggttata
     6721 atcttctaaa gattgttgat actgaatacg tgctgaagcg acttcggcac tgtattgttt
     6781 atcctgtaag tacagtttat tcgtttgccg aacaatattt gccagattat ttttagcttt
     6841 attgagtgcg atagtggcat cagttttatc cagtgaaact aaaatgtcac cctgtcgaac
     6901 gtagttcgta tctttatgat taactacagt gacactacct gagacttgtg cagaaatttg
     6961 atctgcattc cccgtgacat aggcgtcatc tgtactaatc atgtcttcta attccattga
     7021 ccaataggca taggcacctg aaaacgcaat aaataaaact atcaccaata aagaaaagta
     7081 tttttttctg ttagactgtt ttttatttga attaatctgt tccactatta tctctcattt
     7141 cgcatagatg aaatttatga attgaatgaa aagagtattc attcttcata tcaaacacag
     7201 tgcatgtgtt gcacattatc ccaggaacgt tggaggccta atttaaatga ggtgcaaaaa
     7261 aaacaacaga ggactaaacc gtggcttttg caatacaatt cttacgcctg taggattagt
     7321 gagaagactt atagtgctaa cttgaaacta taaatcatcg gtataatccc tgattttatt
     7381 gttgacattt catttatgcc gactatttat atggtatact tgtcgaatta tcttaaagga
     7441 agctcagatt ttcttatttt tattgagaaa atgagatgac gccttatgtc tgtattacta
     7501 cagggagaag ggagatgctt cattgcaaag ggaataatct atgaacgcaa taattattga
     7561 tgaccatcct cttgctatcg cagcaattcg taatttattg atcaaaaacg atattgaaat
     7621 cttagcggag ttaactgaag gcggaagcgc cgttcagcgg gtggaaacac ttaagcctga
     7681 tatcgtcatc attgatgtcg atatccccgg agttaacggt atccaggtgt tagaaacgct
     7741 gaggaagcgc caatatagtg gaattattat tatcgtctcc gctaaaaatg accattttta
     7801 cgggaaacat tgtgctgatg ctggcgctaa tggtttcgtg agtaaaaaag aaggcatgaa
     7861 caatatcatt gcggctattg aagctgcaaa aaatggctac tgctatttcc ccttctctct
     7921 caaccggttt gttggaagtt taacgtccga ccagcaaaaa ctcgactcct tatcgaaaca
     7981 agaaattagt gtcatgcggt atattcttga tggcaaggat aataatgaca ttgcggaaaa
     8041 aatgttcatc agcaacaaaa ctgtcagcac gtataaaagt cgcctgatgg aaaaattaga
     8101 atgtaaatca ctgatggatc tttacacatt cgcgcaacgt aacaaaatcg gctaatcaca
     8161 tgaagttttt accctatttt tttcttctct gttgtggtct ttggtcgacc ataagtttcg
     8221 cagacgaaga ttacatcgaa tatcgtggca tcagtagtaa caaccgtgtc acacttgatc
     8281 cagtacgtct gagcaacaag gaattacgtt ggttagcgag caaaaaaaat cttgtgattg
     8341 cagtacacaa gtcccaaacg gctaccttgt tgcataccga ttcgcagcaa caggttcgtg
     8401 gtattaatgc tgattattta aatcttttaa aaagagcgtt aaatatcaaa ttaacactcc
     8461 gggaatacgc agatcatcaa aaagcaatgg acgcgcttgc agaaggtgaa gtcgatatag
     8521 tgttatcaca tttagttact tcgccgcctc ttaataatga cattgctgca accaaaccat
     8581 tgataattac ctttccggcg ctggtaacca cccttcacga ctcaatgcga ccgcttacct
     8641 caccaaaacc agtaaatatt gctcgggtag caaattaccc cccagacgag gtaattcatc
     8701 aatcatttcc aaaagcaaca attatctctt ttacaaattt atatcaggca ttagcatccg
     8761 tctcagctgg gcacaatgat tactttattg gtagtaacat cattaccagc agtatgattt
     8821 cccgctattt cactcactcc ttaaatgtag tgaaatatta taactcgccg cgtcaatata
     8881 attttttctt gaccagaaaa gaatctgtca ttcttaatga agtactcaat agatttgttg
     8941 atgctttaac aaatgaagtt cgctatgaag tatcacaaaa ttggcttgat acaggaaacc
     9001 tggcctttct gaacaaacca ttagaactca ctgaacatga aaaacagtgg attaagcagc
     9061 atcccaattt aaaggtgctg gaaaatcctt actcgccccc ctattctatg acggatgaaa
     9121 atggctcggt tcggggcgtt atgggggata ttcttaatat tattaccttg caaacaggtt
     9181 taaatttttc tccgatcacc gtttcacaca atatccatgc tggaacacag cttagccccg
     9241 gaggatggga tataatacct ggcgctattt atagtgaaga tcgagaaaat aatgttttat
     9301 ttgctgaagc cttcataaca acgccttacg tttttgtcat gcaaaaagcg cctgacagtg
     9361 aacaaacatt aaaaaaagga atgaaagttg ccattccata ttattatgag ctgcattcgc
     9421 aattaaaaga gatgtatccg gaggttgaat ggatacaggt cgataatgcc agcgctgcat
     9481 ttcacaaggt taaggaaggt gaacttgatg ctctggtcgc gacacagcta aattcgcgtt
     9541 acatgatcga tcattactat cctaatgaac tttatcattt tcttattcct ggcgttccga
     9601 atgcatcgct ttcgttcgct tttcctcgcg gagaaccgga acttaaggat attattaata
     9661 aagcactgaa tgcaattccc ccaagcgaag ttctgcgcct gacggaaaaa tggattaaaa
     9721 tgcccaatgt gaccattgac acatgggacc tatatagcga gcaattttat attgttacga
     9781 cattatccgt tttattagtt ggcagtagcc ttttatgggg attctacctg ttacgctcag
     9841 ttcgtcgtcg taaagtcatt cagggtgatt tagaaaacca aatatcattc cgaaaagcac
     9901 tctcggattc cttaccgaat ccaacttatg ttgtaaactg gcaaggtaat gtcattagtc
     9961 ataatagtgc ttttgaacat tatttcactg cggattacta caaaaatgca atgttaccat
    10021 tagaaaacag tgactcaccc tttaaagatg ttttttctaa tgcgcatgaa gtcacagcag
    10081 aaacgaaaga aaatcgaaca atatacacac aggtatttga aattgataat ggcatcgaga
    10141 aaagatgcat taatcactgg catacattat gcaatcttcc tgcaagtgac aatgcagtat
    10201 atatttgtgg ttggcaagat attactgaaa cgcgtgatct aattaatgca ctcgaggtag
    10261 aaaaaaataa agcgataaag gctaccgtag caaaaagtca gtttctggca acgatgagtc
    10321 acgaaataag aacaccaata agctctatta tgggcttcct ggaacttctg tcgggttctg
    10381 gtcttagcaa ggagcaacgg gtggaggcga tttcacttgc ctacgccacc ggacaatcac
    10441 tcctcggctt aattggtgaa atccttgatg tcgacaaaat tgaatcgggt aactatcaac
    10501 ttcaaccaca atgggtcgat atccctactt tagtccagaa cacttgtcac tctttcggtg
    10561 cgattgctgc aagcaaatcg atcgcattaa gttgcagcag tacatttcct gatcattacc
    10621 tggttaagat cgaccctcag gcgtttaagc aggtcttatc aaatttactg agtaatgctc
    10681 tcaaatttac caccgagggg gcagtaaaaa ttacgacctc cctgggtcac attgatgaca
    10741 accacgctgt tatcaaaatg acgattatgg attctggaag tggattatcg caggaagaac
    10801 aacaacaact gtttaaacgc tacagccaaa caagtgcagg tcgtcagcaa acaggttctg
    10861 gtttaggctt aatgatctgc aaagaattaa ttaaaaatat gcagggcgat ttatcattag
    10921 aaagtcatcc aggcatagga acaacattta cgatcacaat cccggtagaa attagccagc
    10981 aagtggcgac tgtcgaggca aaagcagaac aacccatcac actacctgaa aagttgagca
    11041 tattaatcgc ggatgatcat ccgaccaaca ggctattact caaacgccag ctaaatctat
    11101 taggatatga tgttgatgaa gccactgatg gtgtgcaagc gctacacaaa gtcagtatgc
    11161 aacattatga tctgcttatt actgacgtta atatgccgaa tatggatggt tttgagttga
    11221 ctcgcaaact ccgtgagcaa aattcttcct tacccatctg ggggcttaca gccaacgcac
    11281 aggctaacga acgtgaaaaa gggttaagtt gcggcatgaa cttatgtttg ttcaaaccgt
    11341 tgaccctgga tgtactgaaa acacatttaa gtcagttaca ccaggttgcg catattgcac
    11401 ctcagtatcg ccaccttgat atcgaagccc tgaaaaataa tacggcgaat gatctacaac
    11461 tgatgcagga gattctcatg actttccagc atgaaacgca taaagatcta cccgctgcgt
    11521 ttcatgcact agaagctggc gataacagaa ctttccatca gtgtattcat cgcatccacg
    11581 gtgcggctaa catcctgaat ttgcaaaagt tgattaatat tagccatcag ttagaaataa
    11641 cacctgcttc agatgacagt aagcctgaaa ttcttcagct gctgaactct gttaaagaac
    11701 acattgcaga gctggaccag gagattgctg ttttctgtca gaaaaatgac taaattgcgg
    11761 ctcccacaat gttctaatgt gggagctatt taccagcaca tcttacattt atgatgagaa
    11821 ctcctggcga atttgttccc catgctggtc gagcgttgcc gctcccggca taacatgcgg
    11881 gtccgcgcag ccgctgattt ttatcggatt acccggcatc attattcctc cggcttcaat
    11941 caacatattt ctcgcctgag tttgtggcaa attaatggcc tcagccacac ttaataacgg
    12001 cgcgacgggc acaccaactt catgtatttt ggctaaccaa acttcagctg cctgcgtttt
    12061 taacgttcgc tcaatatatt gtttaagaat agcctggttt tgtacgcgta aaatattgct
    12121 gctaaatcgg ggatcattaa ccagttccgt aagctccagt gcctggcata acgcagaaaa
    12181 aagcttgtca ttaccacaac aaatcgtaat cggcttatcc tgagtatcga aaacatcaaa
    12241 aggtgccatg tagggatggc gatttcccag gcgttgtggt gacttccctg tcgcaatata
    12301 tgccatcaga ccatgctcca gaaaactcag cgtggcatca aacatcgcta tatcgacatg
    12361 cgcccctctc tggctctttt cgcggccata aagtgcactc actattccgc tgaataaata
    12421 gacaccgccg catagatccg caagagaggt accaacacgc actggcggag catcagggta
    12481 ccctgtttcc atcattatcc cactcattgc ctgaatgatg gtatcgtagg caggagcatc
    12541 ttttagcgga ccggtatgtc cgaaacctga cgatgaagca tatatgaggc gcgggttgat
    12601 ttcttgtaac ctttcccatg aaaaccccag tttttccatt gtacctaggc gaaaattctc
    12661 agctaataca tcagcttgtt tgagcatatt tataaatata cttttatcgt gatcattctt
    12721 taaatcaaga accacactct ctttgccatg attaataaaa ctgtaataga gtgactgtcc
    12781 atccacatag ggaccaaatg tgcgggtatc atcaccatga cctggtggct caactttaat
    12841 tacccttgcg cccatattat aaagaagttg agttccgaaa ggtccattaa ggacatgtgt
    12901 catatcgata actaataagc cttcaaacgg ccctttgctt tcattatttg tcatttgctt
    12961 atgccttgaa aaaagccgaa ggtgaaaaga cacttcggca tttatgtaaa atcaataaca
    13021 tgattaatta tgaaaccagt cgactgacat aaatccacaa gggagccgtg acgacaaagc
    13081 ccagtacgct cacagccaat gacgctgtac cagtacgggt gtagacatta aaccggctgg
    13141 caataatgat tccggagaat gccggcggta atgcgcctgc cagtaccatc atctgcaaat
    13201 gttcgctgtt caaatgacat gccataccaa cgagaagcag cgccagtggc atcagaatca
    13261 acttcaggaa ggtgttataa gcaatttcgg cactgaactc gaatttatgt gcagccagag
    13321 tcaaccccgc agcgaatacc gctacccctg agttagcctt cgcaatcaga ttaaaggttg
    13381 ggtcccatgc tgccggaatt tttaccccaa ccagcaccag gatcgttgcc agaacaggtg
    13441 cccatactac tggctccttt gccgcagaaa ttaatgcgct cagattacta ttcttcttac
    13501 catccgctcc tgaggaagga ttcagcaaat acagaccaat aggaatagta attgcgttaa
    13561 caataataga aataattgct accactaaac ctgttgatac ggaatcacca taaataggat
    13621 cgagaactgc aaaccccagg aatccaatgg taggtgaacc cgcaattaat gcacatacag
    13681 ctgcttctgc atgggtacgt ttaaaaaatt tgtagcaaca aaaccaggag aaaaagaaac
    13741 atccgacaat aaccacaagt gatacaaggg tcagacgagt gtccgcaaaa atcatttccc
    13801 tgtttgcccg ggtaatagaa acaaatagag ccgcaggaag agcgtagttt aataccagtt
    13861 tattaaatgc gcgggcttga tcttctgaaa aagtttctcg tctgccgcta aaataaccca
    13921 acagcatgat tacgataata ggcaataaat cgccaataaa aaatgttagc ataatagtat
    13981 ttccgataag ggtcccctca ccatcgcata atgagttggc aagaggagac gatgtcagta
    14041 tttaattacc agcgacttgt tttgggttaa gtttggtaat atggccactt tcagttcctg
    14101 ctgcagggtc gataaccaca ttaataatgg tcggtttgcg cgactgaata ccggtggtta
    14161 aagcatgacg aagttcatct gtcgtggtga cgttatagcc aacgccacga aacgcatcca
    14221 ttaatttgtc ataccttgca tggtgcaaca gatccgttgg tgatggtgca ccagcgccac
    14281 tgagatcaac accgtctcct ctgtagatgc cgccattatt aaaaataacg atcgtcaccg
    14341 gtaggttata tcgacaaatc gtttcaattt ccatcccact gaaaccaaaa gcactatcac
    14401 cctcaatggc gacaaccgga gaaccagaag tcacgctagc accgatggca tagcccatac
    14461 cgatgcccat gacaccccag gtaccacaat ccagacgacg acgtggttta tacatatcaa
    14521 taatatttcg tgcattatcc agggtatttg caccttcatt aactaaataa atatcctggt
    14581 tctcgcgcaa tacgtcgcgc acagcactta atgcattaaa gtaatttaat ggttgagtat
    14641 ctgtacttaa tttttcatgc attttttgtg cattttgctg cttgtggata tttaaaatat
    14701 cgcgccatac cagtggagtc gtaaatgtgt tttgtttcag ttctgccagc ataccttgca
    14761 tactggatgc aatatcacca acgactggca cagcaatggg gcggttgctg tcaatttcct
    14821 gcggttcaat atccagttga ataaactgtg tatctgccgc ccatcctttt ttaccgtgtg
    14881 ccaataacca attcagtcgt gcaccaacaa gcatgacaac gtcagcattt gccagggcaa
    14941 acgaacgcgc agctgccgca gaaagtggat gcgtatcttc aaggatccct ttcgccatag
    15001 acattggcag gaatggaatc tgagcacttt caataaattc acgaagctgt tcatcagctt
    15061 gtgaatacgc cgcgcctttg ccaaggataa ttaatggccg ttcagctttt gctaaaagcg
    15121 aaattgcgct agtgactgac ttcgggcatg gcaataatgc tggcgacgga ttttcaactt
    15181 taacaatcgt ggttaacgct tcgtcttttt ccatcgtcgc ggccaggaca tttgctggca
    15241 aatcaagata aactccgcca gggcgacccg atacagagac ccggatagcg cgtgccaatg
    15301 caatgccaag atcctgcggc tgattaacgc gaaatgctgc tttggcatac ggttttgccg
    15361 cattcatttg gtccagctct tcataatcac cttgctgtag gtcgacgatc gcgcggtcgc
    15421 tggagccgct aatcatgatc atcggaaaac catttaccgt tgcgttggcc aatgcggtca
    15481 aaccattgag gaatcctggc gcagaaactg tcaggcagat ccccggtttt tgggtaagaa
    15541 aaccgcttgc cgcagcggca tagcctgccg actgctcatg acgaaaacca atataatgaa
    15601 tgccttccgc ctgggcatgg cgtgccatat ccgtcacagg aatacctaca acaccataaa
    15661 tagtgtcaat attattctgt tttaatgctt caacgatgat atgcatacca tctgtcattt
    15721 gaagttgatc tgacatgatt aataacctcg aaatttttat aaattgggag ccagaagccc
    15781 ccgttaatat cagatggcgt ggttttgctt cattgcagca atttcatcgt cgctataacc
    15841 cagctcctgc aatacagcag cggtatgttc acctaatagc ggcgcagctt taatatccgg
    15901 cgtaaaggca gagaatttca ttggacaacc aacggtcaga tattttccac gcaacggttg
    15961 ttccacttca acaacactgc cactttggcg caaagaggga tcaagtgaaa tttctttcat
    16021 acttaaaacc ggtgcacaag gaatatcaaa ctgagtcaaa taggccaccg cttcatgttt
    16081 atcaatagtg acagtgtatt tttcgatttc agcaaaaata tcgaaaatat gtggctgtcg
    16141 ggcatgggct gtactgtatg ccggatcggt aatccattct ggtttgccga tggctttaca
    16201 ggtgttttcc cagttttgct cctgaatagt gaaataaata taggcgttag gatcggtttc
    16261 ccagccttta catttcagga tccagccagg ctgaccgcca ccacctgcat taccaccgcg
    16321 gggaactgca tcaccaaatg taccattcgg atactgcggg tattcttcca gataccccaa
    16381 tttatcgaga cgctgctggt cacgtaattt cacgcggcaa aggttcaata cggcatcctg
    16441 cattgacatg gtgactcgtt gcccacgccc cgttttttcg cgatgcagca aagcagcaag
    16501 taaaccgatc agcaaatgca ttccggtgtt gctgtcaccc aacgctgcag cgcttaccag
    16561 cggcggacca tcccaaaaac ccgtagtgga tgccgcgcca cccgctgcct gagcaacgtt
    16621 ttcataggct tttacattca cataaggcga acactcatca aaccctttga tcgaaccaaa
    16681 aatcagacgt ggattgattt cttgaatatg ctcccaggtg aagcccatgt gatcaatggc
    16741 ccctggatga aagttctcga ctaagatatc agcttcgcgg atcagctttt ccattacctc
    16801 tttgccttcc gctgttttgg tatttaactc aatagaacgt ttgttactgt taagcatggt
    16861 gaagtaaagc gcatcgatat caggaatatc tcgcagttgg tgacgcgtta cgtcaccaac
    16921 gccgggacgt tcaattttaa taacgtcagc gccaaaccag gccagcattt gagtacaaga
    16981 tgggccagat tgcacaccgg tgaaatcgag aactttaatt ccttgaagtg gagttgacat
    17041 agcaatacct attagttaat taaatatgtg ttaatttagc gtttatttaa ggcataacgc
    17101 cctctataaa taatccgcaa aatatcttcg tagcggcact cgatattttt aaattcctgt
    17161 atcaagtact gaaaattatt tcatcgccac ttccaattcc tttctgcatg gtaagtagtg
    17221 aggacgaaaa gaagaatatg ataaatattg attcgtggta atcagagaga gtcgcgacat
    17281 ttgtgatgtt attcttacaa ctgtaagaag cgccggtgat ttacaagtaa gagtatgatt
    17341 acgcttttta aagcgtacca taaagcataa cataataaat gatttgtgat gacacgggat
    17401 tattgcggtt tactttgagg aaaataaaat gcgtgtttat agaaccgcga gcgccaaata
    17461 attttatgac cacaacctgt cgtattgtgg tcataaaaaa ttgtcggctg gagggagtta
    17521 aatcctttac ctcaatatac tgcctgtcag cattagttca caaacagtgc ttcgctatcg
    17581 acgatgattc catcttccgc acctttaagc gccaggtttg cctcgtagta ttgcttttta
    17641 tcaagcagtt tctgcgcatc agcaagtgca ttacgagtct gtttaagcgg catcagatat
    17701 tggttttcca ttacgccaac accggccagg cgaagctctt ccattgcgcc ctttttatcc
    17761 cctttcgcca ttttttcatt ggcaattttt atcgcggctt ctttttcggg tgttgctaca
    17821 taactctcag aaattccgac agatgcatta atgacaatat attggtcatc attgagattg
    17881 gtctttttac ctggcttagc gaactttgcc cattcggtac tgtcatctga cagcaaagcg
    17941 gaagcttcat tggtcagttc ttttgccttt tccggatcgc catgaaacag agccaggcgc
    18001 gctacctgca cgtcacgcat cgcatataaa ccttgttcag agatacgctc tgctgctgcc
    18061 gttttctggg tttgctgagt ttgctgttgt gccgctaccg gtgcattatc agcagcccat
    18121 acagttgaag atgccagaat tgctgtgacc atcgtagcca taattaaacg tttcatactt
    18181 acctcattct ttaacagttt tagaaaacgc cgccatcata ttgggtgagc atgaaatgag
    18241 aaatcagaga gcaacggtca ttttgaaagg gttatcttac aattgtagtt attatcgcaa
    18301 agcggcattt agtcttacag ttgtaggaat atgcctcctg tcgcagagaa ctattcttat
    18361 ataccacaaa gagatgtcat ttattatatt gatgtaaata ttgattaatt atatgtaaat
    18421 acagccataa catcgtatgg ttctcagtga accattttca ggataacttt ctatgaaagt
    18481 aaacttaata cttttcagct tatttttatt ggtctctatt atggcatgca atgtttttgc
    18541 attttccatt tctggtggtg taagtgagag gagctataaa gagactgaaa aaacatcagc
    18601 gatgacgacc acacactcta caaaacttca gccatcacag gcgattttgt ttaagatgag
    18661 agaagatgtg ccaccattaa acctcacaga agaaatcacg cctacttatc cgacaaaggc
    18721 gaattatctt attcatcctg tgagatagcg tctgtcacat ggcaggtgaa caatcatgtc
    18781 tgatgctgat gtgaacacct gcccaaatga gccatcctga cccactatcg cctgccatac
    18841 cccaccataa agcgaatcat caagccacaa gccggggcaa gaaacataaa tatccccgtc
    18901 accagttcat taccgccagt taaccacatg gtgaagtaat aaatgagtgc agcgaggatc
    18961 accatgccga agaaagaaga gaccgccgat accacgttca gtacctgacc gatgtagccg
    19021 cttacgcaga caatacacag ggcgagaaac atccataaat aaatcatagc tcaacctcct
    19081 gccgcgctaa aaaacctgta acttcagtca ctttaatagt aagacaatcc ttaacctctg
    19141 gcaatttttg ctaagcatca atgaaaacag cacgttaaat gcaacgctat tgcgtgcgga
    19201 aaatttttta ctttgcacga ttaataatca gtagctgaaa gcagtcagcc agtgataaag
    19261 tagagccacc gcattcatgc tcctcgtggt tatgtcctga ctagtcttta cactctttac
    19321 aggaaccatt gtcgtacatg atggcccaac caattgagtg taccgctgtc gacatggaat
    19381 cttctgcgat acaacaattc gtatctacag aaggtaacta tgtttccaca atgcaaattt
    19441 tcccgcgagt ttctacatcc tcgctactgg ctcacatggt ttgggcttgg tgtactctgg
    19501 ctttgggtac agcttcctta tcctgttctc tgctttctcg gcacgcgtat tggcgcaatg
    19561 gcgcgaccat tcctgaaacg tcgtgaatct atcgcccgta aaaacctgga actttgtttc
    19621 ccgcagcatt ctgcggaaga acgcgagaag atgattgccg aaaactttcg ttcactcggc
    19681 atggcgctgg tagaaaccgg catggcatgg ttctggcccg acagtcgcgt acgtaaatgg
    19741 tttgatgttg aagggttgga taaccttaaa cgcgcacaaa tgcaaaatcg cggcgtaatg
    19801 gttgtcggcg tccattttat gtcgctggaa ctgggcggcc gcgtgatggg actgtgccaa
    19861 ccaatgatgg ctacctatcg tccacataat aatcagctga tggaatgggt gcagacccgt
    19921 gggcgcatgc gctctaacaa agcgatgatc ggcagaaata atctgcgcgg cattgtcggt
    19981 gcactgaaga aaggtgaagc ggtatggttt gctcccgatc aggattatgg tcgtaaaggc
    20041 agctccttcg cgccgttctt tgcggtggaa aatgtcgcca caaccaatgg cacctatgtt
    20101 ctctcccgtc tctctggcgc agccatgttg accgtaacga tggtaagaaa agcggattac
    20161 agcggatatc gtttgttcat caccccagag atggaaggct acccgacaga tgaaaatcaa
    20221 gccgctgctt atatgaacaa gattatcgag aaagagatca tgcgcgcacc ggagcagtac
    20281 ctctggatcc accgtcgctt taaaacgcgc ccggtgggag aatcgtcgtt gtacatttaa
    20341 caaactgctc ttactctttc cctcctcaaa tcggccataa taacctcatg gtttttatgg
    20401 cttttttctt aagttttttc cttcgctgaa tatctaatta ttaccgtcag aaattaaact
    20461 gtcgccgata tgagacactt gtaagttact aaaagtaaat aagaaaatgc ctcttgtctc
    20521 gtttcagttt taggcgtaca ttagcgccgt ctcgagcagt ttagctcaga attctggtgt
    20581 gatgcccacg gtaaaataca gggcttttaa ggtcgctttg cccggtgtca actcactgta
    20641 tcaggtgtaa tgaagtcatt caggcgtaac agtaattacg cggagagatg taaagtgaaa
    20701 tatttcttta tgggcatttc ttttatggtc atcgtttggg ccggtacttt tgccctgatg
    20761 atctaaaagc aaactgtgca atgaaaaaca ggagccatca ggctcctgtt tgcatttctg
    20821 acgacggtgt ttattccgcg ttttcgtgaa tatgtttgct gctggcgggt agtaaaccgt
    20881 cggcacggaa catcgcttta atccctctga tggcctgacg aatacggtcg cggttttcaa
    20941 tcagtgcaaa gcgaacatgc gtatcaccat agtcgccaaa gccaatcccc ggcgagacac
    21001 aagccttcgc ttcgttaagg agcttcttgg caaattccag cgatcccatg gccgcatatg
    21061 gttccgggat tttcgcccag acatacatcg aagccttcgg catttcgacc atccagcccg
    21121 cttcatgcag ccctttaacc aatacatcac gacggcgttt gtactgttcg gcaatgtcgc
    21181 gcacgcactg ttgatcgccc tcgagtgccg caatcgccgc cacctgcaac ggcgtaaaag
    21241 tgccgtaatc gtgatagctt ttgattcgcg ccagggcact gaccagcgtt ttgttgccaa
    21301 ccataaagcc gatacgccag cccgccatgt tgtagctttt cgacagcgta aagaactcga
    21361 ccgccacatc gcgtgcaccc ggcacctgca taattgacgg cgctttccag ccatcgtaga
    21421 cgatatcggc ataggccagg tcatggacca ccagcacatc gtagcgtttc gccagcgcca
    21481 caaccttttc gaagaactcc agttccacgc attgcgcggt tgggttcgac gggaagccga
    21541 ggatcatcat cttcggtttc ggataacttt cacgaatggc gcgctccagt tcgttgaaga
    21601 aatcgacacc ttccaccagc ggcactgagc gtacctgcgc ccctgcaatc accgcgccat
    21661 aaatatggat cgggtaactt ggattcggca ccagcaccgt gtcaccatga tccagcgtcg
    21721 ccagcatcag atgcgccagg ccctctttcg aaccaatagt gacgatggct tctgattccg
    21781 ggtcgatttc aacgtcgtag cgatcctgat accagcggga aatggcgcga cgtaaccgcg
    21841 gaatgccgcg tgaagtggag taaccatgcg tgtccgggcg ctgggccaca gtacataatt
    21901 tttcgacgat atgcggcgga gtcgcaccgt ccgggttacc catgctgaaa tcgataatat
    21961 cttcgccgcg ccgacgcgca gccattttca gttcagcggt gatgttaaaa acgtagggcg
    22021 ggagacgatc aatgcgcgta aagcgacgtt cagggcgagt gtcagccata atttcctcag
    22081 attaacgtta gcgcccggac cgtccgagcg acgctgccac aatggtggct gttttgaaaa
    22141 tagcctgatt aatttctgtc tgtccagcgt tttagaacat ttttatcaat ggatttaaat
    22201 gaggaactcc acttcaaaaa cagaaaattt ttctacgcta acaagaaaat taccaccaat
    22261 tttattaaca ttataatttc aataaattta ccagccagaa gtgcattatc aagagataca
    22321 ttgctaatac accatttatc tttcaatccc cttttaaatt tatggttttt cctcaaaaac
    22381 ggcctgctgg tcatcagcct tcaggttacc tatcatagag gtttaatcct tattcagagt
    22441 cacccgtgca cgaaatattc aacatgctgc tggcggtctt cgatcgggca gcgttaatgc
    22501 ttatctgcct gttctttctc atccgtatcc gcctgtttcg cgaactgttg cacaagtcgg
    22561 cgcactcccc aaaagaattg ctcgccgtta ccgccatttt ctcgctgttc gccctgttca
    22621 gcacctggtc cggcgttccc gtagaaggct cgctggtgaa cgtacgtatt atcgcggtga
    22681 tgtccggcgg gattctgttt ggcccgtggg taggcatcat taccggcgtg attgcgggta
    22741 ttcaccggta tttaattgat atcggcggcg tgacggcgat cccctgcttt atcaccagca
    22801 ttctggcggg ttgtatatca ggctggataa acctgaaaat ccccaaagca cagcgctggc
    22861 gcgtcggtat tctcggcggc atgttgtgtg agacgttgac catgattctg gtaattgtct
    22921 gggcaccaac taccgcgttg gggatcgata tcgtctctaa aatcggcatt ccaatgatcc
    22981 tcggtagcgt ctgtatcggc tttattgtgc ttctggtgca aagcgttgag ggcgaaaaag
    23041 aggccagcgc cgcgcggcag gccaagctgg cgctggatat cgccaacaaa acgctaccgc
    23101 tgtttcgcca tgtcaatagc gagtcattac gcaaggtctg cgaaattatc cgcgatgaca
    23161 ttcacgccga tgcggtggcg attactaata ccgatcatgt gctggcctat gttggcgtgg
    23221 gtgaacataa ctatcagaat ggcgatgact tcattagccc gactacccgt caggcgatga
    23281 attacggaaa aatcatcatt aaaaacaatg atgaagccca ccgcacacca gagattcatt
    23341 ccatgctggt gatcccattg tgggagaaag gggtcgtgac cggaacgctg aaaatttact
    23401 actgccacgc gcatcagatc acctcgtcat tacaggaaat ggcggtcggt ctgtcgcaaa
    23461 tcatctccac gcaactggag gtttcacgcg ccgagcagct acgtgaaatg gcaaataagg
    23521 cagagcttcg cgccctgcaa agcaaaatta atccccattt tctgtttaac gctctgaacg
    23581 ctatttcatc gtcaatccgt ctgaatccgg ataccgctcg ccagttgatc tttaatctgt
    23641 cgcgttatct gcgctataac attgaattaa aagacgatga gcaaatcgat atcaaaaaag
    23701 agctgtatca aattaaagac tatattgcca ttgagcaggc ccgctttggc gacaagctga
    23761 cggttatcta tgatattgat gaagaggtga attgctgcat tcccagcctg ctgatccagc
    23821 cgttggtgga gaacgccatt gtccacggta ttcagccttg caaaggtaaa ggcgttgtca
    23881 ccatcagcgt tgcagagtgc ggaaatcggg tacgcattgc ggtgcgagat accgggcacg
    23941 gcatcgatcc aaaggtgatt gagcgggtcg aagcgaatga aatgccgggc aataaaattg
    24001 gcctgctgaa tgtccatcat cgcgtgaagt tattgtatgg cgaggggctg catatccgcc
    24061 gcctggagcc ggggacggaa attgcgtttt acattcctaa ccaacgcacc ccagtcgcct
    24121 cacaggctac gttattgctt tgagccggag tgatattgtg aaagtcatca ttgttgaaga
    24181 cgaattcctg gcacaacagg aactgagctg gctaattaaa gagcacagcc agatggagat
    24241 tgtcggcacc tttgacgacg gtctggacgt gttgaagttt ttgcagcata accgcgtcga
    24301 cgccattttt ctggatatca atattccgtc gctggatggc gtgttgctgg cgcaaaacat
    24361 cagccagttc gcccataaac cgtttattgt gttcatcacc gcgtggaaag aacatgcggt
    24421 agaagcgttt gaactggagg cgtttgacta cattctcaaa ccatatcagg agtcacgtat
    24481 taccgggatg ctgcaaaaac tggaagcggc ctggcaacaa cagcagacca gcgtacgcct
    24541 gccgcgacgg taacgcgtga gaatgacacc attaatctgg tgaaagatga gcgaataatc
    24601 gtcacgccaa ttaacgatat ctattacgcc gaagcgcacg agaaaatgac ctttgtctat
    24661 acgcggcgtg aatcctacgt aatgccgatg aacattaccg aattttgcag caaactgccg
    24721 ccgtcgcatt ttttccgctg ccatcgctca ttttgtgtca atctgaacaa aatacgcgaa
    24781 atcgaaccgt ggtttaataa cacctacatt ctgcgactga aagatctgga ttttgaagtg
    24841 ccggtcagcc gcagcaaagt gaaagaattt cgccagttaa tgcatcttta atcgggaact
    24901 ttcatgaaag caccaggctt gcccgccgat caacaatttt tcgccgatct gttcagcggc
    24961 ctagtgctta acccgcaact actggggcgc gtctggtttg ccagccagcc tgcctcgtta
    25021 ccggtgggca gtttatgtat tgattttccc cgtctggata tcgtgctgcg cggcgaatac
    25081 ggcaacctgc tggaagcaaa gcagcagcga atggtggaag gagaaatgct gtttattccg
    25141 gcacgcgcgg ctaatttacc gatcaacaac aaaccggtga tgctgttaag cctggtgttc
    25201 gcaccgacct ggcttgggtt atcgttttac gatagccgca ccacgtcgtt gttgcatcct
    25261 gctcgccaga tccagcttcc cagcctgcaa cgcggtgaag gtgaagcgat gcttaccgcc
    25321 ctcacccatc ttagccgttc gccgctggag cagaatatca ttcagccact ggtgttaagt
    25381 ttgctgcatc tttgccgcaa cgtggtgaat atgccgccgg gtaattcgca accgcgcggc
    25441 gattttctct atcacagcat ttgtaactgg gttcaggata attatgccca gccgctcacc
    25501 cgcgagagcg tggcgcagtt ttttaatatc acgcccaatc atctgtcaaa actgtttgct
    25561 cagcatggaa cgatgcgttt tatcgagtat gtgcgttggg tgcgaatggc gaaggcgagg
    25621 atgattttgc agaaatatca tctgtcaatt catgaagtgg cacagcgttg cggttttccg
    25681 gatagcgact atttttgtcg cgttttccgg cgtcagtttg gtctgacgcc gggagagtac
    25741 agcgcccgtt ttcagggcta acgtcagaag gttaattctg tttccagcag cgtcaggata
    25801 ctttgtgcat cttgcgcggc aaacaacgac tggcggaagt ttttattcac cagtttacgc
    25861 gccagctgcg agaagacttt cacatgattc atcccttcgt tagcacccag cgtcagcatg
    25921 atcaccagtt cgacttcgcc catttctgac tgccagtcaa ccggtttcgc cagccgggca
    25981 atgctgatac tggaatgacg gatccactga gatttggtgt gcgggatcgc tacgccaaaa
    26041 ccaacgccgg tggtaacaat ctcttcccgc tgccagacat cttcttccag ctcaaacgga
    26101 tgttcagtgc gcccgttaac gccgaggttg ccgcacagga actggatcgc ctgctcttta
    26161 ttgctaagat cctgatcaac aaagatattc tccagcgcca gcagtgggcg aacgtcttct
    26221 tccggcgtaa aggcggtgag taacgcttca atttcctgcg cactgcggca ttcacatgcc
    26281 tgacgcgcca gttcccgaca cgcctcgcta tccagttgac gaagctggct tttcaccgcc
    26341 ggaatacgcg ggctactcat actcagctca tccaggccca gcccaagcag tagcggcaga
    26401 taacggcttt caccgcccag ttcaccgcaa atgcctaccc atttgccccg ctgatgcgca
    26461 gtggtaacta tttgctgcaa catgcgcagg aacgatggcg taatcgggtt atatagcggc
    26521 gatacgcgcg ggttattacg atcgaccgca tacagatact gggtcatatc gttggagccg
    26581 atactgaaga aatcgacctc atcgcagaag tggtcgatga tgtagcacac cgacggcact
    26641 tccaccatga tcccaagcgt aatcgtctct gcatgacgca ggccatcgcg cttaagctca
    26701 acgatcgctt tttggatctc gcctttcacc cataagatct gatcgaggct gtgaaccatc
    26761 gggatcatca actgggcgtt gccgaaactg gcggcgcgca aaatggcccg cagttgagtg
    26821 cggaacaggc cagcaaattc cgggtaaata cgtaccgcgc gatagccgag gaatgggttc
    26881 tcttcctggg gaacattcag ataagaaatg cttttatcgc cgccgatgtc catcgtgcgg
    26941 aagataatcg gcttgtcgcc cgccgccagt agcacctgct ggtaggcttc aaattgctcc
    27001 tgctcgtccg gcgcgctgtc gcgatccata tagagcattt cggtacggaa cagcccgaca
    27061 ccttccgcgc cgttggcaaa cgcgcctggc gcttccagag cggtgccgat attcgccgca
    27121 atatcaatac gcttgttatc acgggaatag gccagctgcg cggcagcctg cgcctgttgt
    27181 ttttggcgtt tatccgccag cgtctgcgcg acctgataat aaccgctcac cgcgtcattc
    27241 gggttaatcg ccagcacgcc gcactgggcg tcaagcactg caggttgccc ggcataacgg
    27301 gcaatcgcat ccagcggcaa gccactcagt accgggatcg ccgaggcacg ggccagaatc
    27361 agtgtatgcg aggtgcgccc ggttttctcc agaatcatac ccgcaagatt tttcaaatca
    27421 aggctcaaaa actggcttgg ggttaaatct tcagccacca gaatggtcgg tttttccagc
    27481 accagattgt tgcgcggctt cagttccggc caggtgatat gcagcaactg ttcgctgata
    27541 tcgcgaatgt cgctgacccg ctcgcgcaga taatcgctgg cagaagcaga tagtttggcg
    27601 caaacctgct ccatattgct gatgatcgcc gcccccagtc cctgatgctg ttctgtcatc
    27661 aggcgacgga tattgcctgc aaattcatca tcctgaatca gcgacaaatg agcgctgagg
    27721 atagttttgc tttcgccgtc acgctcacgc aattgctgat tcagttgctc ggcaagcgtt
    27781 gccaggctgt gctccagccg ggtggaatct tgcgcactgg cggggattgc ccgataactg
    27841 tcgaggctgt cgctttgtaa cagggtcagc gtacccacgc cgacgccgct tgccagcaca
    27901 ttgccgtaca gtaaatccgg gttaaggcgg cttaatgaac gcggcagcgg atgcgccgtc
    27961 agttctgcct gcgtcggctg aacgctgtcg ctgtcgataa agcgcaccag gatgtactct
    28021 tccagcacgc gccgcgcctg ctcttcatcg ctgccgctaa tgttcaggct gcaactgtca
    28081 ttaaataagg tgccagttcc aatcaacgcc agcgagcttt tagcatcggc ctttgcgttc
    28141 tggcgatggt tgataaaggt gatttcgctt tgccactggc tgcactgttc tttaagttcc
    28201 cgcgccggac gagcatgtag accgttaggc ataggcaaag gacagagaaa ttgaatcgtt
    28261 aacataaccg ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc
    28321 tcacgcgtaa gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg
    28381 caatcggcaa tcgatgccgc gcaatgtccg tggcgggtgg caggccccat taccaatgtg
    28441 ggtacgccag taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata
    28501 tccgcctgca acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt
    28561 ggcggcgcaa tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga
    28621 tggttagccg cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg
    28681 acgtccggcg acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg
    28741 gaacttgcca ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac
    28801 gtcaccagca gatagcaacc gaggcgatca tcaaaggctt tgcccatcac ccgctggtga
    28861 gggaggacct gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc
    28921 atcacttcgt cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc
    28981 tgccggtcgc cgtcaagcag gcctagaatt ttgcactctt cacgggtggt gatgcgcacc
    29041 ggctgcagct ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct
    29101 tcgcgggaga tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc
    29161 ttcggacctg tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact
    29221 tctttttgca ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag
    29281 gcgatcgcat ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg
    29341 tgagcaacac tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca
    29401 caacatcttc gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca
    29461 gcaacatgcc tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga
    29521 cttcaatgcc gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagactcgg
    29581 taatgacccg gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag
    29641 agattgccgc gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt
    29701 cggcgctcac cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga
    29761 tacagcgcac caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct
    29821 ttgccgtgcg gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt
    29881 tctgcgccct gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc
    29941 cccgcctgaa taaagcggcg aatatgctcc gctccgcaat cggcaatccc acaggcgagg
    30001 cggattatct ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta
    30061 accagtttcg cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg
    30121 ccttcaaaac cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta
    30181 agcgtgttcg tcgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca
    30241 acgaaatagc gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct
    30301 gatccggtgg agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca
    30361 tccagttgtt gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc
    30421 cttcttacag gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca
    30481 ccaccatcag cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaggt
    30541 tagtgaccag cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc
    30601 agacagctgc ggttgagccg acaatcgcgc cgaccatata tgacggaatg gcggtgatgg
    30661 ggctttccag cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg
    30721 cggtcttacc ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt
    30781 caataatggt cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg
    30841 tgaccggtaa tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga
    30901 tcgggccacc aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc
    30961 ccttctcacc tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg
    31021 gggtgatgac gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa
    31081 atgtggtttt gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca
    31141 caaggtaacc ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg
    31201 aagaggtcgc ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag
    31261 acatcaaccc gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca
    31321 caaatgcagc aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca
    31381 gccaggcaaa tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat
    31441 ccatgatgcc gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag
    31501 cgaggatcac accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat
    31561 gctgcgggag ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg
    31621 ccgcaccgga tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta
    31681 tccctgtcga atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc
    31741 ccgcagcgtt tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac
    31801 gttcgttatc ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat
    31861 cctgcgccgt caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat
    31921 aaccggcttc taccgccgct tcttccagcg cctgagcggc cataaaagtg tgagccaggc
    31981 ccattgggca ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg
    32041 atagttgacg agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg
    32101 catttactgg agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg
    32161 gacacaaaga aaaattgaat taactgtatg atttaaaata ttatcgggag agttacctcc
    32221 cgatataaca ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga
    32281 accgagaaga cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc
    32341 tttaaagcgc cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag
    32401 gaagcgcggc acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc
    32461 cagattgccg ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc
    32521 ggtgcagctg tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag
    32581 gcggttgtca gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac
    32641 gcgctccgcc gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc
    32701 ttcactattc ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt
    32761 atcgacatgg accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg
    32821 cttaccttcg accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg
    32881 gatcgccatc gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt
    32941 ctttttcatt tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc
    33001 ggtaattggg caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag
    33061 ataaacgcga atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg
    33121 cgagatttca ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc
    33181 accgactaat gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt
    33241 ctcacactgt aaatacttgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa
    33301 tatcgatctg ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt
    33361 tgcaagctgg gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc
    33421 gagccagaac catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc
    33481 ttattctgat tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac
    33541 tgccgggaac tctggggata gcccaggatt cccccctctg gatcatcggt gtattaacgc
    33601 taacgggtat tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc
    33661 ccgcctgtga accgctgatc ggcgcaccgg ttccgccctc tgcgctacct ggacttatcg
    33721 tagcattaat tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga
    33781 ccgtcaatat cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa
    33841 tggagtggac tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg
    33901 cggcggcgtt gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc
    33961 gtctttttgc gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc
    34021 atccccattt ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca
    34081 gcggtgcaat tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac
    34141 cacggttgca cgcgataatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg
    34201 gatttattct cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg
    34261 atgagatgca gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg
    34321 cggtaattaa acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa
    34381 tcttcccggc agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg
    34441 ccgttccagc ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac
    34501 gcgatggctg gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac
    34561 cgctgctctg tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga
    34621 tggtcaatcg tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct
    34681 ttatgattta agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa
    34741 caggccgctg acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg
    34801 cggccgccct gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc
    34861 catcgatcaa attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc
    34921 actggcgaga tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc
    34981 gtcatgataa tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc
    35041 gtgaaggctg gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc
    35101 attaaacgcg tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg
    35161 cctacaaacc ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat
    35221 agtgccagat acgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga
    35281 tattcagcgc cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt
    35341 tcaccagatc gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca
    35401 gggcgatacc aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc
    35461 ccatcagaat gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga
    35521 tatggaagcg aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg
    35581 aggacagtcc ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca
    35641 gcggttgcag cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt
    35701 ggaacgccgc cgcagctgta gccatcatcg ccagattgac aaaaccggca atcgtcatgg
    35761 cgatagccac atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat
    35821 gctgagtgag cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc
    35881 ctgctgccag gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca
    35941 gctgcgccag gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca
    36001 acagtaaccc gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa
    36061 tcaggaaagt cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga
    36121 gtttaaaacc gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt
    36181 ctgcctgaac ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg
    36241 ccagattttt accggtggca atccctagtt tggcagagag gatctgaatt agcatcgcca
    36301 tcaggttggc ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa
    36361 tattggtcgc aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca
    36421 ttaatgcgag cctcatcttg cgcgccgccc gtccgctgct actctcaacg cgatagttcg
    36481 tcatcttgtg cctctaaaac atagcctttg ctatgtttca tgctattcta aacgagaatg
    36541 attatcaaat tcatttaaat ggattgtggt gatttctctg atagaccaag attacgatta
    36601 cgaaaagatt gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact
    36661 ttcagcttcc atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt
    36721 cacaggacgt cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat
    36781 gtgcacggaa atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt
    36841 gtactggcac ttgccgttgt tgcgattctc gcactgctgg taagcagcga ccgcaaaaaa
    36901 attcgtatcc gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc
    36961 ctgaactccg acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaacta
    37021 ctcggatttg ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg
    37081 gcattcttct tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt
    37141 ctccagcaca ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa
    37201 gtcaacggta tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag
    37261 tcagaaaact ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac
    37321 accatggctg ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc
    37381 atgctggaac cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc
    37441 gtgctgtcgc tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc
    37501 aacctgcacg aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc
    37561 aaagttgcca ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg
    37621 aacgcactgt ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc
    37681 ctgggctaca tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg
    37741 caagtgggca gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat
    37801 ctgcagaaaa ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttcctg
    37861 gtttccttcg ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat
    37921 gaagagcaag gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg
    37981 gtgagtgtgc tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc
    38041 cggggatcat tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa
    38101 tcaaataccc ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg
    38161 tctcgacaaa ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc
    38221 tcacaatcat cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt
    38281 tgatgatatc agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt
    38341 catcaatcgc aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt
    38401 ctgcgttaga aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt
    38461 gataacgctt aaacagacga ataatccgtc cggcaatatt cttttgcagc agcgtgagcg
    38521 gcattaaatt gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact
    38581 tcaacaagga ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa
    38641 ggggcagaaa cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct
    38701 catcataacc ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct
    38761 gatctaacgc cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa
    38821 ccatctcctc tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca
    38881 gccagcttaa ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc
    38941 aggcagcacc atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca
    39001 ggatgttaac catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca
    39061 gttcactacc cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac
    39121 ggcaaattga gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact
    39181 caagattatc aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt
    39241 ccaacgcacg aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc
    39301 ggcgattaag ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa
    39361 aggaaatcag caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa
    39421 agttctggtt gtaattcaga aggcaaagcg ttgaaacagc ctaggtgaga tttaaaaacg
    39481 gatagcgaag attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac
    39541 cggcaataaa gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg
    39601 ccagccagct taaggtaaat gcgcgtttct ctttgcccaa cgacggagcg atatccctgc
    39661 gccacaatat ctgcgcaaag tggggactta caatcatgcg agtgagatag tagaaaagca
    39721 tgttgtaaat cagcactgcg gtgaaaaggc ttagtaaatc aacgaccgtg aaaatggcat
    39781 ccgcatcgcc gaaaaaggtg gatatcttta gcgggaaatc aaagaagctc cccacaagat
    39841 acatgctgca tttgatgccg atcggcgtca ccaaaccaag ccagaacaga cgttgccaga
    39901 catatcggct ggtcaatcca taacgccaac gcgtccccag ctgccagcgt aatattgcgc
    39961 aggcacacaa cacgacaaac gtctggctgc cgagcatgac cgcagtctgt aaaaaagaga
    40021 gatgaaagtt ccacagattg gtgcaaaaca tcccgcataa aacgggaacg acaccgcgcc
    40081 agccaaagat aaacaagatt gacagcatta cgcacagtgg catccacgcc agaaagatat
    40141 aactggaatg gataatggca agtggcgaaa taaaacggga tagctgaata agtaccacgg
    40201 tgagcgtaaa cgctagtgtg aatatcttga tattttttat caggttatgc tccacaaaca
    40261 tgagtacact actctcactg atcggggttt tcaggcacac aaataataag tttattcgca
    40321 tcgcgaagca aacaggggcc agcctgttgc cgatttttca tccaatgatg ctttcaccag
    40381 gcggcgctaa cccggacgta aactgtatta caaaagcggc aaaaagcaga gacaaaaaac
    40441 ccccgctttg cagcgagggt tggaaatttg gtggagctaa gcgggatcga accgctgacc
    40501 tcttgcatgc catgcaagcg ctctcccagc tgagctatag ccccacgatg cgtttacgta
    40561 ccaaatttgc tgggtgcaaa atttggtgga gctaagcggg atcgaaccgc tgacctcttg
    40621 catgccatgc aagcgctctc ccagctgagc tatagccccg tcacgtaaag cttgtcgagt
    40681 tgacgggcgg catcatatga attccgcccg aatgtgtcaa cggcaaattg caacgtgtag
    40741 tttcaatcgc tgaaaaatca ggcaaatgaa caattttgga aaaccgctcg cattcagtag
    40801 ttattcatgt cacggtttcc tgtaaagtgg tgttataaaa tgaactacta atagacccac
    40861 atacattcag ggaattgtta tgtccaagga gaggatgaca ccagatgaac ttgccagact
    40921 gaccggctat agccgccaga ccattaataa atgggtacgc aaggaaggct ggacaacgtc
    40981 accaaaacct ggcgtccagg gtggtaaagc ccgactggtt cacgtcaatg aacaggttcg
    41041 tgaatatatt cgcaatgctg aacgtccaga aggtcaggga gaagcgcctg ccctttctgg
    41101 tgatgcgccg cttgaagtat tgctggtgac actggcgaaa gagatgacgc cagttgaaca
    41161 aaaacagttt acatccttgc ttctgcggga agggattatc ggattgttac aacgcttagg
    41221 gattcgcgat agcaaataat atgaaaagat tacgcaataa aatgaccacc gaagaactgg
    41281 ctgaatgcct cggtgtggct aaacaaactg ttaaccgttg gatcagagaa aaaggctgga
    41341 aaacggaaaa atttcccggc gtgaaaggcg gtcgtgccag acttattctg gtcgatacgc
    41401 aagtttgcga gtttattcag aacacgccag ccttccataa cacaccaatg ttgatggaag
    41461 ctgaagagag aattgctgaa tacgctcctg gagcacgcgc cccggcgtat cgacaaatca
    41521 tcaacgcgat agataatatg acagacattg agcaggaaaa agtcgcgcaa tttttgtcac
    41581 gtgaaggaat tcgcaatttc ctcgcccgtc tcgatattga cgaatcagca taaataaaaa
    41641 acggcaggat attatctcct gccgtttatc tttttacacg ctaattactg ctgattttcg
    41701 cgttcagcaa taaaatccag cgctttgttg atacgctcga tactgcgggt cttaccaatt
    41761 gcgtgaacgg taacatccag tgctggagac tgccccgcac cggttacggc gacacgcagc
    41821 ggcataccaa ctttacccat acccacttcc agctcatccg ccgtcgcctg aatagcgtga
    41881 tgaacgtttt cagcggtcca gtcagtaatc gcggccagtt tgtcacgaac cacttccagc
    41941 ggctgacgcg ctaccggacg cagatgtttt ttcgccgcat cggcatcgaa ctcagcaaaa
    42001 tcttcgtaga aataacggca gctctgtgcc atctctttca gcgtcttgca gcgttcgccc
    42061 agcagtttca ccagatcagc cagctgcggg ccgttacggg tatcgatatt ttcctgctca
    42121 atgtgccact gtaagtgagt agcaacatac tccggcggca gcgcgttaat gtagtgatgg
    42181 ttcagccaca gcagcttgtc ggtgttgaac gcactggcag atttgctgac ggcattcaaa
    42241 gtgaagtatt tgatcatctc ttcacgagtg aagatttcct gatcgccgtg ggaccagccc
    42301 agacgcacca gatagttcag cagtgcttct ggcaaataac cgtcatcacg atactgcatt
    42361 acgctgactg ccccgtgacg tttggacagt tttttaccgt catcgccatt gatcatagaa
    42421 acgtgcgcgt aaaccggcac cggcgctttc agggccttaa gaatgttgat ctggcgcggc
    42481 gtgttgttga tatggtcttc gccacggata acgtgggtga tttccatatc ccagtcatcg
    42541 acaaccacac agaagttata ggttggggaa ccatcggtac ggcggataat aagatcgtcc
    42601 agttcctggt tgctgaactc gatcggacca cggatctgat cgtcaaaaac aacagaacct
    42661 tcctgcgggt tagcaaaacg tacaacacac ggttcatcat cagcatgatg ctcatggctg
    42721 tggcggcagc gaccgtcata acgcggcttc tcacctttcg ccatttgctc ttcgcgcagc
    42781 gcttccaggc gctctttaga gcaatagcat ttataagcag tgccctcttc cagcatctga
    42841 tcgatcaccg cgttgtagcg atcaaaacgt ttggtctggt agtacggacc ttcatcccac
    42901 tccaggctca gccagttcat gccatccata atggcttcga tagcttccgg cgtggaacgc
    42961 tcaagatcgg tgtcttcaat acgcagcacg aactcaccgc cgtggttacg tgcaaaaagc
    43021 caggagtaaa gagcagtacg cgcgccgcca acgtgcagat agcctgttgg gcttggcgcg
    43081 aagcgagttt tgattttcat gaaatggcct tacgtttaga aagatgccga caaccggcaa
    43141 atcctggaaa aattgagtgg gcgatattct atcactcccg cctgattcct caatgtagtt
    43201 cgggtttcta ccttacgcat tattgttttc gttgacaaat tgcgcaacac ggtctgtttt
    43261 gcgatcatct tgcttaaatt tacgacgaac gaacaatttc tttagaaaat gcgttgactc
    43321 attttgaact ctccctataa tgcgactcca cacagcgggg gtgattagct cagctgggag
    43381 agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc accaactact
    43441 ttatgtagtc tccgccgtgt agcaagaaat tgagaagtgg gtgattagct cagctgggag
    43501 agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc accacttcgg
    43561 gtcgttagct cagttggtag agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc
    43621 tgcacgaccc accaatgtaa aaaagcgccc taaaggcgct tttttactat ctgcgatact
    43681 caaagattcg aacctgcagc aggtttgagt tgagcgcagc gaaacaacgg agccgctcgc
    43741 ggcgacggcc cgaagggcga gcgaagcgag tcatcctgca cgacccacca atgcaaaaaa
    43801 gcgccctaaa ggcgcttttt tgctatctgc gatttgtaag attgattttt cttctcaagt
    43861 tccttgttct tctaccgata ttacctcgta cagtgacgag gactgaacaa tggccactat
    43921 ctcttcaact tcaattcctt ccatacaaac acagagtagt aacagggcat cacaaggtag
    43981 cgatgttgct tcacaaatcg cgcggataag tcagcaaatc atcaaactca ctcaacaaat
    44041 taaagagatt gtagacacta gcggcagtgc tgaagacaag caaaaacagg ccgaattgat
    44101 tcaacagcaa atcaccctac tggaaacaca actggcgcag ttgcagaaac agcaggcaga
    44161 aaaggcgcag gagaaagagc agcgtttgtc tctgaatgta tcccttctaa atcctgttga
    44221 aaaaacgact catatcgata tctatatctg atacaacgga tccccttccc gaccagccat
    44281 tagcgcattg ccctggtctc gtaccacgtc ccataacgct tcagcggcgg tggaaagtga
    44341 acggttcttc ctgcgcacca gcatcaactg gcgttcaacc accggcgtaa tgcgcttaac
    44401 caccagtggg ctaccttcag gcaatggtag cgccagtgca ggaagaatac tgatgccgat
    44461 gcctgcggct accatcggga acagcgtcgc cggatgtcca atctcctgca caatattcgc
    44521 ctgaatacca ttgcgcgcca gcgctgcgtc aatcagcggt cggctgcctg acgcgtaatc
    44581 ctgcaacacc aatttcgctc cctgtaatgc ctgccagggt acataatctt ccacggccaa
    44641 agcgctatcg cgatggcaaa gcagaaagaa aggctcggaa agaatcgctt cacattgcag
    44701 atcgcccact gggcctggat cgatgacgat gccaaagtcg acatctccct ggcgaatact
    44761 ttccatcacc cactgctgcg gacgatcatg caggacaaac tgaatatctg gatagcggcg
    44821 atggctttca gcaatgcatt gcggaataag atgcgcggaa atggtctggc tggcagcgac
    44881 ccgaactttt ccgctcagtt gttgccccat acgcccggta tcgcgcaacg tgctgttaag
    44941 ttcatccagc agtcgctcaa gacgcaaagc caactgctgc cctgcatctg taagcaccac
    45001 ttcacgcgtg gttctgtcca gcaggcgaac accagtatga ttttccagct ccttcacact
    45061 gtgacttact gccgactggc tcaagccgat acgctctcct gcacgactaa aacttttctc
    45121 ctgcgctact gtgacgaaaa cctttaattg ttttaaagaa taattcatct gttttcttca
    45181 tgaatggatg caataaatca attttatttc tcaaactgga agaagcacaa tagaaccatc
    45241 gatcatctgg agtctttatg aaactttttc gtatcctcga tcctttcacc ttaaccctga
    45301 tcacggtggt gttgctggcc tctttctttc cggccagagg cgatttcgtc cccttctttg
    45361 aaaatctgac caccgcagct attgccctgc tgttctttat gcacggcgcg aagttgtcgc
    45421 gtgaggcgat tattgctggc ggtggtcact ggcgactgca tttgtgggta atgtgcagca
    45481 ccttcgtgct gtttccgatt ctgggtgtac tgtttgcctg gtggaaaccg gtaaatgtcg
    45541 acccgatgct ctactccggt tttctctact tgtgcattct cccggctacc gtgcagtctg
    45601 caatcgcctt cacgtcaatg gcgggcggta acgtcgcggc ggcggtttgt tctgcgtcag
    45661 catccagcct gctggggatt ttcctttcac cattgctggt tggtctggtg atgaacgttc
    45721 acggtgcaag gggcagcctt gagcaggtcg gtaaaattat gctgcaactg ctgctgccgt
    45781 ttgtgttggg gcatctttcc cggccgtgga ttggtgactg ggtgtcgcgc aataaaaaat
    45841 ggattgcgaa aactgaccag acgtccattc tgttggtggt ttatacagcg ttcagcgaag
    45901 ccgtcgttaa tggtatctgg cataaagttg gctggggatc attgctgttt atcgtggtgg
    45961 tcagctgcgt tcttctggct atcgtgattg tagttaacgt ctttatggca cgccgactgg
    46021 gcttcaataa ggcagatgaa attactatcg tcttttgtgg ttcgaaaaag agtctggcaa
    46081 atggcatccc gatggcaaac attctgttcc ccacatcggt gatcggtatg atggtgctgc
    46141 ccctgatgat tttccatcag atccaattga tggtctgtgc ggtgctagcg cgtcgataca
    46201 aacgccagac cgaacagtta caggcgcagc aggaaagcag cgccgataaa gcttaaagcg
    46261 gacgcttcag gggctggacc agttgcgtca gcccctcggt tttaatcagc agcgtgattt
    46321 gcattagctc accgagcttc cctgccggga actcatcttt gcgggcaaac cacagcagat
    46381 actcctccgg caggtcaatt aagcgacgcc ctttgtattt gccaaacggc attatcgtat
    46441 tggctatttc aatcagctgc tctttttcca tctcagctac ccagcaaacg cagcatttcc
    46501 gcttcgtcga tgacttcaat gcccagttcc tgcgccttcg ccagtttaga tcctgcagct
    46561 tcacccgcta tcaccagatc ggttttcttc gacacgctgc ccgcgacttt cgcgcccagt
    46621 tcgaccagtc gagctttagc gtcatcacgc gacatctggc ttaagctgcc cgtaagcacc
    46681 acggttttac cagcaaacgg gctgtcaatc tcttccgcgt tgataacgat cggcgcaggc
    46741 cagtgaacac cttccgccaa cagctcgctg atgacattgc ggttgctttc ttcggcaaag
    46801 aagttgtgaa cgtgggatgc aacgacaatg ccaacatcag gcaccttttg cagctcttca
    46861 atcgaagcgg cttccagcgc ttccagcgtg ccgaaatatg ccgccagacc tgctgcggtg
    46921 gcctcgccga cttcacggat gccaagtgca tagaggaagc gagcaaaggt ggtttctttc
    46981 gctttttcca gcgcgttaac cacgttttgt gccaattttg gccccatacg ctccagtccg
    47041 gtcagtttgc ctgcggtgag tttgaacaga tctgccggag tatggacata ttctttttca
    47101 accagctgat cgatgatttt gtcgcccatt ccgtcaacat ccatcgcacg gcgggaaaca
    47161 aagtgtttca gcgactcttt acgctgcgca ccgcaaatca ggccaccggt acagcgggca
    47221 accgcttcac cttccacacg ctcaacgtca gaaccacata ccggacaatg cgtcgggaat
    47281 acaacctcac gggtatcttc cgggcgttca gaaagcacga cgttaaccac ctgcgggatc
    47341 acgtcgccag cgcggcgaat caccacttta tcgccaatgc gtaaaccaag acgttcgatt
    47401 tcatccgcat tgtgtaaggt tgcgttactc accagcacgc cagcaacatg gacaggttcc
    47461 agacgcgcaa caggcgtaat cgcgccagta cgcccaacct gaaactcgac gtcacgcaca
    47521 aaggtcatct gctcctgcgc cgggaattta aacgctaccg cccagcgcgg ggcacgcgcg
    47581 acaaagccaa gctgctcctg ctgttccagt gagttgacct taatcaccac gccgtcgata
    47641 tcaaagccca gcgtcgggcg gtcttcttcc actttgtgat agaacgccag cacttcttcc
    47701 gccgattcac aaagcgttac ccgatcgctg accggcaacc cccacttttt aaattgcagt
    47761 aaacggccaa gatgagtatc cggcagctcg cccccttcca gaacaccaac gccatagcag
    47821 aaaaaagtga gcggtcgctt cgctgtaata cgcggatcaa gctgacgcag tgaaccagct
    47881 gccgcattac gtgggttagc aaaaactttc ccgcccgtgc gtcgcgcatc ttcgttaatc
    47941 ttttcgaacc ccgcctgcgg caggaacact tcaccacgca cttccagacg cgccgggata
    48001 ttctctccgt gcagcttcag cggaatggcg cgaatagtac gcacattaga cgtgatatct
    48061 tccccggtgg tgccatcgcc acgggtcgcg gcactgacta aaacgccatt ttcatacaga
    48121 atactgacgg caaggccatc cagcttcagc tcacagcacc aggtgacttt ctcgttgttt
    48181 ttcagacggt cctgcacacg tttgttgaaa gcaagaaagc tttcttcatc aaaaacgtta
    48241 tccagtgaca gcattggtac ttcatggcgt atctggctga aagctgccag cggcgcagca
    48301 cctacacgtt gggtaggcga atcaggcgta atcagttctg gatgtttggt ttccagctcg
    48361 cgcagttcgc gcatcagcct gtcgtattca gcgtcgggaa tttccggcgc atccatcaca
    48421 tgataaagat attcatgatg gcaaagcgtc gttcgcagtt ctgtcagttg ttgttcgatt
    48481 gattccatat cgcaccatca atgctaaaaa cccccgacaa gcgggggttc gaagaggagt
    48541 taatttgcct taagtgtatc aggcgttggc gtctttgact tcgcggatga tgtcctggta
    48601 ctcgcgcaat ttctgcggag tcatcatact gcgctgatcg tcaagcacga caccgcccac
    48661 ttcatcggca atatgctgcg cagattgcag catcagcttg aagttctgca gctcgtcacc
    48721 gtaagacggt acctgcataa agatagtgac acccggagta gtgaaatcct tcatttcagg
    48781 atcaaaggtt cccggtttca ccatattcgc caggctgaat aacgccgggc cgctgccatc
    48841 cgggctaaga tgacgatggt aaatattcat atcgccaaaa atgaagcccg cttgttgaat
    48901 gctgttaaga agcagttcac cgtttagctc gctaccgtga tgcgccgcga cgttcatgat
    48961 aatcaccgct tctttgcgct tcggtttatc cataactgga gccggttccg ctacaggctc
    49021 aggctgtggt gccgctacgg gttctgcagg ctggaaagcc tgttgtgccg gttgcggtgc
    49081 tgaatgcact ggctgcggcg ctggcgcgac ctgtggcgaa actggctgct ggaccggttg
    49141 acgcggctgc gcagacgcat aaggcggttg gtactggtgt tgcggcgacg gacgagcagc
    49201 ctcatgctcc tgagcgttag ccggggcatg attcacgcgg tgaacacgaa cctcaccaac
    49261 gccctcatca tcttcgacat cctcgtcata agaatcgtcg tcacgttttg acttcattcg
    49321 ttttaatggc cgatcgcgga acatagaaga tcgttcttta cggctggtcc agaaaccatg
    49381 taccagtaaa gcgattatgg cgatcgcgcc aacaatgatt aatatcagac gcaaatcctg
    49441 catcattata ttctctgttg ttctaacacc ttgccaccac ggcaaacatt tactcactaa
    49501 gagtatttgc cgattacctc aagtgcaagt gcactattaa ctttcacagc acaaagatag
    49561 atgaaatcgt gctttttgct gttttttcga acatatccta actgtccatt gcgcaattac
    49621 ccggtaaaat acgcagaatt ttcctgggat tggtcaaaag gagctcatcc tgattatggt
    49681 ttcatcattc acatctgccc cacgcagcgg tttttactat tttgcgcaag gctggaagct
    49741 cgtctcgcaa cctgggattc ggcgtttcgt tattttaccg ctgctggtca atattttgtt
    49801 gatggggggc gcattctggt ggctctttac acagctcgat gtctggatcc cgactttcat
    49861 gagttacgtt ccggactggc tacaatggct gagttatctg ttgtggcctc tggctgtcat
    49921 ctctgtgctg ttagtgtttg gctatttctt ctccacgatt gctaactgga ttgccgctcc
    49981 gtttaacggt ttattggctg aacaactgga agcacgattg actggcgcta caccgccaga
    50041 taccgggatt ttcggtatca tgaaagatgt gccgcgaatc atgaaacgcg aatggcaaaa
    50101 atttgtctgg tatctgccgc gcgcaattgt attactaatt ctttacttca tccctggtat
    50161 tgggcaaacc gtcgcgccgg tactgtggtt cctgtttagc gcctggatgt tagccatcca
    50221 gtattgcgat taccccttcg ataaccacaa agtgccgttt aaagagatgc gcaccgccct
    50281 gcgcacacgc aaaatcacca atatgcagtt tggtgcttta accagcctgt ttacgatgat
    50341 cccgctgctt aatctgttca tcatgcccgt tgccgtttgt ggcgcgacgg cgatgtgggt
    50401 cgattgctat cgcgataaac acgcgatgtg gcggtaacaa tctaccggtt attttgtaaa
    50461 ccgtttgtgt gaaacagggg tggcttatgc cgccccttat tccatcttgc atgtcattat
    50521 ttcccttctg tatatagata tgctaaatcc ttacttccgc atattctctg agcgggtatg
    50581 ctacctgttg tatcccaatt tcatacagtt aaggacaggc catgagtaag atttttgaag
    50641 ataactcgct gactatcggt cacacgccgc tggttcgcct gaatcgcatc ggtaacggac
    50701 gcattctggc gaaggtggaa tctcgtaacc ccagcttcag cgttaagtgc cgtatcggtg
    50761 ccaacatgat ttgggatgcc gaaaagcgcg gcgtgctgaa accaggcgtt gaactggttg
    50821 aaccgaccag cggtaatacc gggattgcac tggcctatgt agctgccgct cgcggttaca
    50881 aactcaccct gaccatgcca gaaaccatga gtattgaacg ccgcaagctg ctgaaagcgt
    50941 taggtgcaaa cctggtgctg acggaaggtg ctaaaggcat gaaaggcgca atccaaaaag
    51001 cagaagaaat tgtcgccagc aatccagaga aatacctgct gctgcaacaa ttcagcaatc
    51061 cggcaaaccc tgaaattcac gaaaaaacca ccggtccgga gatctgggaa gataccgacg
    51121 gtcaggttga tgtatttatt gctggcgttg ggactggcgg tacgctgact ggcgtcagcc
    51181 gctacattaa aggcaccaaa ggcaagaccg atcttatctc tgtcgccgtt gagccaaccg
    51241 attctccagt tatcgcccag gtgctggcag gtgaagagat taaacctggc ccgcataaaa
    51301 ttcagggtat tggcgctggt tttatcccgg ctaacctcga tctcaagctg gtcgataaag
    51361 tcattggcat caccaatgaa gaagccattt ctaccgcccg tcgtctaatg gaagaagaag
    51421 gtattcttgc aggtatctct tctggagcag ctgttgccgc agcgttgaaa ctacaagaag
    51481 atgaaagctt taccaacaag aatattgtgg ttattctacc atcatcgggt gagcgttatt
    51541 taagcaccgc attgtttgcc gatctcttca ctgagaaaga attgcaacag taatgccagc
    51601 ttgttaaaaa tgcgtaaaaa agcacctttt taggtgcttt tttgtggcct gcttcaaact
    51661 ttcgcccctc ctggcattga ttcagcctgt cggaactggt atttaaccag actaattatt
    51721 ttgatgcgcg aaattaatcg ttacaggaaa agccaaagct gaatcgattt tatgatttgg
    51781 ttcaattctt cctttagcgg cataatgttt aatgacgtac gaaacgtcag cggtcaacac
    51841 ccgccagcaa tggactgtat tgcgctcttc gtgcgtcgcg tctgttaaaa actggcgcta
    51901 acaatacagg ctaaagtcga accgccaggc tagactttag ttccacaaca ctaaacctat
    51961 aagttgggga aatacaatgt tccagcaaga agttaccatt accgctccga acggtctgca
    52021 cacccgccct gctgcccagt ttgtaaaaga agctaagggc ttcacttctg aaattactgt
    52081 gacttccaac ggcaaaagcg ccagcgcgaa aagcctgttt aaactgcaga ctctgggcct
    52141 gactcaaggt accgttgtga ctatctccgc agaaggcgaa gacgagcaga aagcggttga
    52201 acatctggtt aaactgatgg cggaactcga gtaatttccc gggttctttt aaaaatcagt
    52261 cacaagtaag gtagggttat gatttcaggc attttagcat ccccgggtat cgctttcggt
    52321 aaagctctgc ttctgaaaga agacgaaatt gtcattgacc ggaaaaaaat ttctgccgac
    52381 caggttgctc aggaagttga acgttttctg agcggtcgtg ccaaggcatc agcccagctg
    52441 gaaacgatca aaacgaaagc tggtgaaacg ttcggtgaag aaaaagaagc catctttgaa
    52501 gggcatatta tgctgctcga agatgaggag ctggagcagg aaatcatagc cctgattaaa
    52561 gataaacaca tgacagctga cgcagctgct catgaagtta tcgaaggtca ggcttctgcc
    52621 ctggaagagc tggatgatga atacctgaaa gaacgtgcgg ctgacgtacg tgatatcggt
    52681 aagcgcctgc tgcgcaacat cctgggcctg aagattatcg acctgagcgc cattcaggat
    52741 gaagtcattc tggttgccgc tgacctgacg ccgtccgaaa ccgcacagct gaacctgaag
    52801 aaggtgctgg gtttcatcac cgacgcgggt ggccgtactt cccacacctc tatcatggcg
    52861 cgttctctgg aactgcctgc tatcgtgggt accggtagcg tcacctctca ggtgaaaaat
    52921 gacgactatc tgattctgga tgccgtaaat aatcaggttt acgtcaatcc aaccaacgaa
    52981 gttattgata aaatgcgcgc tgttcaggag caagtggctt ctgaaaaagc agagcttgct
    53041 aaactgaaag atctgccagc tattacgctg gacggtcacc aggtagaagt atgcgctaac
    53101 attggtacgg ttcgtgacgt tgaaggtgca gagcgtaacg gcgctgaagg cgttggtctg
    53161 tatcgtactg agttcctgtt catggaccgc gacgcgctgc ccactgaaga agaacagttt
    53221 gctgcttaca aagcagtggc tgaagcgtgt ggctcgcagg cggttatcgt tcgtaccatg
    53281 gacatcggcg gcgacaaaga gctgccatac atgaacttcc cgaaagaaga gaacccgttc
    53341 ctcggctggc gcgctatccg tatcgcgatg gatcgtaaag agatcctgcg cgatcagctc
    53401 cgcgctatcc tgcgtgcctc ggctttcggt aaattgcgca ttatgttccc gatgatcatc
    53461 tctgttgaag aagtgcgtgc actgcgcaaa gagatcgaaa tctacaaaca ggaactgcgc
    53521 gacgaaggta aagcgtttga cgagtcaatt gaaatcggcg taatggtgga aacaccggct
    53581 gccgcaacaa ttgcacgtca tttagccaaa gaagttgatt tctttagtat cggcaccaat
    53641 gatttaacgc agtacactct ggcagttgac cgtggtaatg atatgatttc acacctttac
    53701 cagccaatgt caccgtccgt gctgaacttg atcaagcaag ttattgatgc ttctcatgct
    53761 gaaggcaaat ggactggcat gtgtggtgag cttgctggcg atgaacgtgc tacacttctg
    53821 ttgctgggga tgggtctgga cgaattctct atgagcgcca tttctatccc gcgcattaag
    53881 aagattatcc gtaacacgaa cttcgaagat gcgaaggtgt tagcagagca ggctcttgct
    53941 caaccgacaa cggacgagtt aatgacgctg gttaacaagt tcattgaaga aaaaacaatc
    54001 tgctaatcca cgagatgcgg cccaatttac tgcttaggag aagatcatgg gtttgttcga
    54061 taaactgaaa tctctggttt ccgacgacaa aaaggatacc ggaactattg agatcattgc
    54121 tccgctctct ggcgagatcg tcaatatcga agacgtgccg gatgtcgttt ttgcggaaaa
    54181 aatcgttggt gatggtattg ctatcaaacc aacgggtaac aaaatggtcg cgcctgtaga
    54241 cggtaccatt ggtaaaatct ttgaaaccaa tcacgcgttc tctatcgaat ctgatagcgg
    54301 cgttgaactg ttcgtccact tcggtatcga caccgttgaa ctgaaaggcg aaggcttcaa
    54361 gcgtattgct gaagaaggtc agcgcgtgaa agttggcgat actgtcattg aatttgatct
    54421 gccgctgctg gaagagaaag ccaagtctac cctgactccg gttgttatct ccaacatgga
    54481 cgaaatcaaa gaactgatca aactgtccgg tagcgtaacc gtgggtgaaa ccccggttat
    54541 ccgcatcaag aagtaattct tgccgcagtg aaaaatggcg cccatcggcg ccatttttta
    54601 tgcttccgcc agcggcggca aaatcaattc atcgctctca tgctgctggg tgtagcgcat
    54661 cacttccagt acgcgcaacc ccgcacggtg caccgcatcg tttaacgcct tccctttcag
    54721 caaaccactg ataagctgag cacaaaacag gtcgccagtc cctttcaggt cggtttttac
    54781 ccgtgaatgg gaaatgacat tcacgctgtc ggcactgacc accaccacct gcatctcctg
    54841 attttcttca ttaccggagg cgctggtaat caccacccat tttaatgtgt ctgaaagcag
    54901 actttttgcg gcagcaatgg cactgtcgag atcgcggcaa tttttaccgg tcaggatttc
    54961 cagctcaaag atattggggg taattccctg cgccagcggc agtaaatatt gtcgatacgc
    55021 ctcgggaagg tcaggtttga cataaattcc gctatcaata tcgccaatca ccggatcgac
    55081 catgatcaat aggtcaggat ggtctttgcg tagcgcagtc agccactcgg caaggatttt
    55141 gatttgcgat gccgttccca tatagcccgt ggttacagca cgaagttggc gcagcgcatc
    55201 acgctcctga agcgcacgca aatagccgct aaaccattcg tccggaatcg caccaccgta
    55261 gaaagtgtca taatgcggcg tattgctcag caataccgtc ggcacggcaa agacattcaa
    55321 gccgttctgt tttatagcag gcaccgcaat gctgttgcct acgcttccgt acactacctg
    55381 cgactgcacg gcgacgatat ccgcctgcag cgccctactc ttatcgttaa acaacaacaa
    55441 actactcatt taattttttc tccttgccga tgatcctcat cgtattccaa ccgaaacttt
    55501 acctgattct ggcagtcaaa tcggctatta caaaacaaag ataaggtaat tcagtgaaga
    55561 aaatcatttg tctggccatt acgctgctaa tgacactccc cgcttacgcg aagttaactg
    55621 ttcatgaaga agcccgcatc aacgccatgc tggagggatt agcacagaaa aaggatttga
    55681 tatttgtgcg caacggtgat gaacatacct gcgatgaagc ggtttcccac ctgcgtctga
    55741 agctcggcaa tacccgtaac cgcattgaca ctgccgagca gtttattgat aaggtagctt
    55801 cgtcgtcatc gattaccggg aagccgtata tcgtgaagat ccccggtaag agcgatgaga
    55861 acgcacagcc ttttttacat gcgttaattg cgcagacgga taaaacggtg cctgcggaag
    55921 gaaattaatc cgctttggta aggcatttac aggaggtaac atgaaaaaat gctttattta
    55981 tcacgatgaa aaatcgaata aattttggtg gatagattac gaaggggata gtttagctgt
    56041 caactatggc aaggtaggta gtattggtaa attccataca aaagaattcg ataatgaaga
    56101 acagtgtctg aaagaagcca gtaaattgat tgccgcaaaa atgaaaaaag gatatcaaga
    56161 aactccagac tttaacttca tggatctcta ctattttgat gatgaagaga ttgggttaca
    56221 tcttaaaacg tcacacccaa acttccagtg ccattttact gatccacttt atatgtgttg
    56281 ctgggatgaa gaatctcctt ttggtagcga tgaaggtgct gatgctctaa acgaacttga
    56341 aaatagcctc cgttctggac tgtgctgatt tccctcaaat gttaattgaa actatgtggg
    56401 gtatgaaata catcgctatg gacagtattc ttgaagagga tgtgcgtgcg caattactag
    56461 ccgatgaaat gagctctatc cagagcaata tgattaccta cgcgactgca ttcggtcaga
    56521 tcaaagtcat gggtaaaatc tcccataaac ttaaaaagat gggactcaat gcactagcgc
    56581 gtcatcagct taccgcaaaa attcttcaat ggggtgacgg tcaggactca ccaatacttc
    56641 aaaaaatgat tgatgacctt acttcttttc cacacgaaaa ttaaaaacta catttgtcgg
    56701 cagcaacaac tgccgacaaa tcagattatt gacaaagtgc actttgttta tgccggatgc
    56761 ggcgtaaacg cctgatccgg cataaaaata ttgctaattc aatatattgc agaaacattg
    56821 taggcctgat aagcggagcg catcaggcag ttttgcgttt gtcatcagtc tccaatgcta
    56881 ttaatcctta aatccctgcc ccctgactaa aatgctcttc cccaaacact ccggtggaga
    56941 ggtagcgatc gccacgatcg cagatgatcg ctaccaccac cgcaccaggg ttagcttttg
    57001 ccacccgcag tgctccagca accgcgccgc ctgagctgac gccacagaat attccttccc
    57061 gcaccgccag ttcgcgcatg gtgttttccg catcgcgctg atgaatatcc agcacctcat
    57121 ccaccagcgc agcgttgaaa atccccggca gatattccgc aggccagcgg cgaatgcccg
    57181 gaatactgct gccttcttcc ggttgcaggc caacaatggt caccggtttg gattgttcgc
    57241 gcataaagcg tgaaacgccg gtgatagtgc cggtcgtccc catgctggag acaaaatgag
    57301 tgatgcgccc gccggtttgc tgccagattt ccggcccggt ggtggtgtaa tgcgcataag
    57361 ggttatcggg attattgaac tgatcgagca gctttccttc gccacgattc gccatctcca
    57421 gcgccagatc gcgcgcacct tccatgccct gctctttggt gacaagaatc agttccgcac
    57481 cataagcatg catcgccgca cggcgttcct ggctcatgtt gtcgggcatc agcaatttca
    57541 tgcgatagcc tttcagcgcg gcaatcattg ccagcgcaat gccggtgtta ccactggtgg
    57601 cttcgattaa gacatcaccc ggtttaattt ccccgcgctt ttccgcctcg acgatcatcg
    57661 aaagtgccgc acgatctttc accgaacctg ccgggttatt gccttccagt tttaaccaca
    57721 cttcactgcc gttatccggc cccattcgct gcaacttcac cagaggcgta ttgcctattg
    57781 tttgttctaa tgtactcacg atctctatcc atacgtggtg ttgcctgatg cgacgcttgc
    57841 gcgtcttatc aggcctacag gttacaaacc ttgccattaa aaagcccgga tcgcgggaag
    57901 cgcctccggg cgtttaacat tcactcaacc tatcaggcgc tttgtgcgag agcaagttcc
    57961 tcatcgcggg tttcaatacg ctcgtcgccg ttatacagcc gcgcatgttg cagaccaacg
    58021 aataaacgct cgccacgctg cggggcatcg tcgccatgca tcacgaccgt cagcggttcg
    58081 ttgtaccacc ccagcggctg caccactaat tgggtgtagt gacctttcgg gctggcttcc
    58141 agtacctgta ccggcagcgg cgaatcgaga ctggtacggc ggctgatatc cacttcccac
    58201 gggcgcagga agagatccac cggaccctga tacgcaggtg tgtagcccag cggccagcga
    58261 tgcgcgccaa catggaactg cccgccgcga atggttccct gcaggcggtt cacttcgccc
    58321 ataaattcga gtacaaaacg ggtcgctggt tcgcgccata cctgattcgg cgcatcagcc
    58381 tgttcaatat tcccctgact catcaccact acacgatcgg ctacttcggt cgcctcttcc
    58441 tggtcgtggg tcacaaacac gctggtgaat ttcagctctt catgcagttg acgcagccag
    58501 cgacgcagct ctttacgcac ctgcgcatcc agcgcgccaa acggttcatc aagcagcaga
    58561 atttgcggtt ctacagccag cgcgcgcgcc agcgccacac gctgtttctg gccgccggaa
    58621 agctgcgccg gataacgatc cgccagatgc gcaagctgga ccatttccag caattttgtc
    58681 actttcgctt tgatggctgc ggcattcggg cgctcgcgac gcggcagcac cgtcaggcca
    58741 aaagtgatat tgtcgaatac cgtcatatgg cggaacagcg cgtaatgctg gaacacgaaa
    58801 ccgactttac gatcgcgcgc gtgcaggcga ctcacgtcgg tgccgtggaa gcgaatatgc
    58861 ccgctggttt gatgctccag cccggcgata atccgcagca gcgtggtttt cccggaaccg
    58921 gacggcccca gcaacgcgac catctgacca gaaggaatat ccagtgagat atcgttcagc
    58981 acctgggtgc gaccaaaaga cttcttgata ttggcaatct caatgctcat gatgctcctc
    59041 ctgctgcgcg cgtttttcct gattctccag gcgccactgc aacatactct ttaaaaacag
    59101 ggtgataatc gccatcagcg tcaacagcgc ggcggcggta aaggagccga cggtgttgta
    59161 gtcctgctcc aacaattcaa tctgtaacgg cagcgaaagt gtttcgccgc gaatcgagcc
    59221 ggaaaccacc gacacagcac caaactcgcc aatcgcgcgg gcgttggtca gcaccacgcc
    59281 gtaaagcagc gcccagcgaa tattcggcaa tgtgacgcga cggaacatct gccagccgga
    59341 cgcgccaagc aaaatcgccg cttcgtcttc ctggctgccc tggcttaaca tcaccggcac
    59401 cagctcgcgc accacaaacg gacacgtcac aaagatggtg accagcacca ttccgggcca
    59461 ggagaacata atttgcagat tatgttcatc cagccagccg ccgagcgggc cgttggagcc
    59521 gtagaacagc agatacacca gaccggcaac caccggcgat acggcaaacg gaatgtccag
    59581 tagcgtcagc agtaactggc gtcccgggaa gttaaagcgc gtcaccagcc aggccagcag
    59641 aatgccgaac accaggttta ccggtacggc aatcagcgcg atcatcaccg tcagccagat
    59701 ggcgtgcagc atgtccggat cggccagatt ctgtaaaacc ggcatcagcc ccttgctgaa
    59761 tgcctgcacg aagatgtaaa tcatcggcac cagcaggatg aacgccgaaa ccagcatccc
    59821 gatgccaatc agaaaccatt tgccccagtt aatcgggcgc gcgtcataac gcttcaattg
    59881 ggtaacttcc gccattaatg acctaccaca cgccgaccaa agcgactttg cagagtgtta
    59941 attgagaaca gcagcagcag agatgccgcg aggatcaccg aagcaatcgc gctcgctgcc
    60001 gggtaatcaa actcctgtaa gcgcacaaaa atcatcagcg acgtcacttc cgtcttccac
    60061 gcgatatttc cggcgataaa aatcaccgcg ccaaattcac caagactacg ggtaaacgac
    60121 agcgccacgc ccgccaccag cgccggagaa agctccggca gcaccacttt gcagaaactc
    60181 tgccagcgcg ttgcaccaag cgtttccgcc gcttcttcat attccgggcc taactcttcc
    60241 agcaccggct gcacggtacg caccacaaac ggaatgctgg taaaggccat cgccaccgca
    60301 ataccaagcc aggtataggt gactttgata tcaaacttcg ccagccattc accgtaaaaa
    60361 ccgtttacgg aaaagagcga ggccagcgtt aaaccggcga ctgccgttgg cagcgcaaag
    60421 ggtaaatcca tcagcgcatc aagcagcgtg cggcccggaa agcgataacg tgttaaaatc
    60481 cacgccatca gcagaccgaa aacgccgtta aaaatcgatg ccacaaacgc cgacagcagc
    60541 gtcactttgt aggccgcgac cacctgcggg ttggtgatca cctcccagta ctgcgcccag
    60601 ctcatctcag aaagctgcat caccagcgca gagagcggca gcagcaatat caggcacaca
    60661 aacagcagac tggtgccaag gcttaaagta aagcccggca gcacgcgtct tgaagaaact
    60721 gcaaacatta cttacgcccc gccgctaaca gcttgtctaa ctcgccgccg ctggtgaagt
    60781 gggttttcat cacttccggc catgagccaa atttgtcttc cacgcggaac agttcggtct
    60841 gcgggaattt atctttcagt ttgtccatga cttccgggtt attcacacgg tagtaatagt
    60901 cggtgatgat ggtctgcgcc tgtgggctgt agagccagtt caggtaggct tttgcggctt
    60961 tttccgtacc gttggcctgc acatttttat cgacccacgc caccgggaat tccgccagaa
    61021 tattggtttt cgggatcacc acttcaaagc cctgtgcttc atactgttta cggatgttgt
    61081 tcacttccga ttcaaagcta atcagcacat cgcccaagcc acgctcggca aaagtggtgg
    61141 ttgcgccacg accgccggta tcgaacactt caacgttttt caggaactgg gtcataaact
    61201 gttcggtttt ggcttcatcg ccaccgtcag ctttatccgc tgcgccccat gccgccagat
    61261 aggtataacg cgcgttaccc gacgttttcg ggttcgggaa aatcagcttc acgtcggagc
    61321 gcaccaggtc gttccagtcg tggatattct tcgggttgcc cttacgcacc aggaagccca
    61381 tggtggagta gaacggcgag ctattattcg gcaggcgcga ctgccagtca gccgggatca
    61441 gcttgccttt gtcgtgcagg atttgtacgt cggtcacctg gttataagtg acaacgtctg
    61501 cttttaagcc ctgcaaaatc gccagcgcct gttttgatga cccggcatga gattgtttta
    61561 tcgtcagttt gtcgccgccg ttatcttttg cccattgctg ttcaaacggc ggattcaggg
    61621 cggcaaacag ctcgcgggag acgtcataag aactgttcag cagttccgtt gcctgtacat
    61681 ggcccgccag cagcagagaa gcgaccagcg cgagtgagtt ctttttcagt aagttaacgg
    61741 ccattgcgca cccttataaa tttaatgact ttctaatagc catcatattt ataacggacg
    61801 tgaaaggagt aacggtttta tataccgttt ggtgatttgg aagttaaaaa gggaataaga
    61861 acttggttca acaggcgtaa ttgttgcagg cagtttggac acggacagcg cggagaaacc
    61921 ggagcgtaca catagtacgt gaggatttcg agcactgccc ggggccaaaa tgacaaatga
    61981 aatagcctgg tgaacttagt tcaagacgaa atcctcccca cacatgcagg gaggaaacag
    62041 aggtgaatca gataccgacg ctaaccgtct ccggcagtgt gctgccgcca tcaatcacat
    62101 tctgtgtacc ggttaaatag ctggattcat ccgatgcgag gaaggccgcc agttcgccga
    62161 cttccagcgg atcggcgagg cgacacagcg ggattgcttt tgccatttca gtcagcaccg
    62221 attctggatc ttccgggttc gactggcggg caatgctttc cgccattggc gtgcggacgt
    62281 atcccgggca gatggcgtta acgcgaatac cggattgcgc gtactccacc gccagcgatt
    62341 tagtcaggcc aacaatcgcc gctttcgtta aggcatacgc cgtttcgcca ggatcggcca
    62401 ccatatcacc agtgactgaa gacatcatca caatgcgacc atctttgcgc gcaatcatct
    62461 ccggcagcac cgccttcgtg acgttccata cgcctttaat attgatatca atatggaaat
    62521 cgcgatcttc atcgctcata tcgaggaaac tgcccagacg acaaacgcct gcgttattca
    62581 ctaggatatc aatgcgccct tctttttcct tcgcgcgttt gatagctgcg gctaccgacg
    62641 ccgggtcacg cacatcggcg acaaccgccg tacagcgatg accacgacca cacagttcgt
    62701 ccgctagctt ttcgatctca ggggagatat ccagcaagat taggttcgcg ccatgacgtg
    62761 caaaagttct ggcaattcct tcgccaattc cctgcaatgc gcccgtaatc agtgctgtct
    62821 tgcccgtgag tttacccatt ttaatgctct ccttgttaat acagagcgtt acactccatt
    62881 ccctctgaaa atacaggata aacaaaatat tgctgcggat cagctcacta attcaggaaa
    62941 atctcaatcc agtcgctgct ttatgcatca tttcaggcgt tgtgttagct cactgctgcg
    63001 ctgaatcatt ttcaatgacg ccacatcatt gatgttgcac aagaccaaca aacagtaaat
    63061 cggtaacaga attttgcgcc gtgcgggtag acatcgacga gctacgccat tcggtttcac
    63121 cggaaacggt atcgagcgtg aaatgcgcca gccgccgcag cggagagtca gccagagaag
    63181 taatggcaat caccgttgcc ccctgctttc gtgccgcttc tgcgcacaag acaatctctt
    63241 ttttactgcc gctgtaagag atagcgattt gtacatcgcc ttttttcaac gcctgggaaa
    63301 cggtggcttg tacgtgcgtg tcggcttcgc aggccacgcg atagccaatt ttcatcagtt
    63361 tgaacgacag atcgcgcccc accagcgccg atccgcccag gcccgttatc tggataaacg
    63421 gtgctttact gatgacctca atgatttttt gcagccgcgc gtagtcgaac aacgcacagg
    63481 tttgctccag cgccagctct ttttcgcgat tcagtttgcg ggcaatcact tccagcgaat
    63541 catcactggt aatcgaactg tgcaggtgca gcgccgtggc attcgttttc tcccggctgg
    63601 cactgtattc gccaattaac gccatacgca attcagtaaa tccttgcgcg ccgagcttct
    63661 gggcaaattt cacaatgctc gactggctga taccgagctg tttcgccatc tgccgtgaag
    63721 agacggattt cagctcgctc actctggccc gcagaaagtc agcaattttt tgctcgttcc
    63781 cggtaaattc actttccgca ttgcgtattt tcgtcaggta caacatcttc cactctcctg
    63841 ggctggcagc aaaaattccg cttaagttct tatgacgctc ttcacactct gcgagtgtag
    63901 actcaatgat tcctttaagt ctgtcaattc gggatatatg attccataca tctgcaatta
    63961 tttgatcgta aatagtaagg tcaccaccga tgcaactaga aaagatgatt actgaaggct
    64021 cgaacaccgc ctcggctgaa attgaccgcg tatcgacgct ggaaatgtgc cggattatca
    64081 acgatgaaga taaaaccgta ccgcttgccg ttgatcgcgt cctgccggat atcgccgcgg
    64141 cgatcgatgt tatccacgcc caggtcagcg gcggcggtcg tctgatttat ctcggtgcgg
    64201 gaacatccgg tcgtctgggg attctggatg ccagcgaatg tccgcccacc tacggcgtga
    64261 aaccgggtct ggtggttggt ttgattgctg gaggcgaata tgccattcag cacgcggtgg
    64321 aaggcgctga agatagccgg gaaggtggtg ttaacgatct gaaaaatatt aatttaacgg
    64381 cacaggatgt ggtggttggc attgctgcca gcggtcgcac gccgtatgtg attgccggac
    64441 tggaatacgc acgccagctc ggctgccgca cagtgggaat ttcctgtaat ccggggagcg
    64501 ccgtttcaac caccgctgag tttgccatta caccgattgt aggtgccgaa gttgttaccg
    64561 gttcttcgcg gatgaaagca ggcacagcgc agaaactggt gctcaatatg ctttccaccg
    64621 ggctgatgat taaatccggc aaagtgttcg gcaacctgat ggtcgatgtg gtcgccacca
    64681 acgaaaaact gcatgtgcgc caggtcaata tcgttaaaaa cgccaccgga tgtaatgccg
    64741 aacaagcgga agcggcgtta attgcctgcg agcgcaactg taaaacggcc attgtgatgg
    64801 tgctgaaaaa tctcgatgcc gcagaagcta aaaaacgcct ggatcaacac ggcggcttta
    64861 ttcgtcaggt tttagacaag gaataaccca tggccaaaga gatcagcagt gaacttctga
    64921 acaccattct tacccgtgtc ggcggaccgg gaaatatcgc cagttgtggt aactgtatga
    64981 cgcgcctgcg tctgggtgta catgacagtt cactggttga tcccaatatc aaaacgctgg
    65041 aaggcgtgaa gggcgtcatt ttgaccagcg accaggtgca ggtcgttttt gggcctggta
    65101 aagcgcatcg tgccgcaaaa gcgatgagcg agctgctggg cgaggcaccg gtacaggatg
    65161 ccgcagaaat cgccgcccag aacaaacgtc agttaaaagc caaacaaacc tccggcgtgc
    65221 aacaatttct cgccaaattc gccaccatct tcacgccgct gatccccggt tttattgccg
    65281 ccggtctgct gctggggata gcgacgttaa ttgccacggt gatgcacgtt ccggcagatg
    65341 ctcagggcac gctacccgac gcgctgaatt ttatgaaggt gttcagcaaa ggtttgttca
    65401 ccttcctagt gattctggtg ggctataacg ccgcccaggc atttggcggc acgggcgtca
    65461 atggcgcaat tattgccgca ctctttttgc tcggttataa ccccgctgca accaccggtt
    65521 actacgccgg ttttcacgat ttctttggtc tgcccatcga tccgcgcggc aatattatcg
    65581 gcgtgctgat tgccgcgagg gcctgcgccc gcattgaagg catggtgcgc cgctttatgc
    65641 cggacgatct cgacatgctg ctgacctcgt taatcaccct gctgatcacc gctacgcttg
    65701 cgtacctgat cattatgccg ctgggtggct ggttattcga aggtatgtca tggctgttta
    65761 tgcacctgaa cagtaatccg ttcggttgtg cggttttagc cgggctgttc ctgatcgccg
    65821 tggtatttgg cgtgcatcag ggctttattc ctgtttacct cgcgttaatg gacagccagg
    65881 gattcaacag cttatttccc atcctttcaa tggcaggcgc gggccaggtg ggcgcggcgc
    65941 tggcactcta ctggcgggcg caaccgcaca gtgcgctacg cagtcaggta cgcggggcga
    66001 ttattcccgg cctgctgggc gttggtgaac cgctgattta cggtgtcacc ctgccccgca
    66061 tgaagccgtt tgttaccgcc tgtttaggcg gcgcggcggg cggtttgttt atcggattga
    66121 tagcctggtg gggtctgccg atgggcttaa acagcgcctt tgggccgtct ggtctggtgg
    66181 cgctgccgct gatgacttcc gcacaaggca tccttccggc aatggcggtt tatgctggcg
    66241 ggattctggt ggcatgggtt tgcgggttta ttttcaccac gctctttggc tgccgtaacg
    66301 tcaatctgga ctgatattat gaaacggaca atgctctatc tttctctgct ggctgttagt
    66361 tgtagcgtca gcgccgcaaa ataccctgtt ctgacagaag gtagttgatc aagtatatgc
    66421 ggcgttaaag cagtaataaa aaggccagtc agtaatgtta ctgcctggcc tttaatcaat
    66481 gaatcagaaa cgcttacagc accatcaact tgtccagcga cggtgcgaaa taatagccgc
    66541 cggttaccgg tttggtgaaa cgcaacatcg catcacgttt gccatcggta tcgccaaaca
    66601 tgctcagcag ttgctgctca atgttatgca gacgcgcgca gtaggcgcag aagtacagac
    66661 cgtgagtgcc actggcggtg ccgtacggca ggctctggcg aacaatcttc agccctttgc
    66721 catcttcttt cagatcaacg cgggtgaggt gagaggtttc cggacgttcg tcgccgtcaa
    66781 tctcttcgtt ggcctctttg gtgcgcccga tcatcatctc ctgatcgtga acgctcatcc
    66841 ggttgagctg cttcaggttg tgctcccaac gctgcacaaa cacatagctg ccgccggcat
    66901 ccacgccgtc tttgataacc gccacttcgc ggcgcgtctc ttcacccgcc gggttttctg
    66961 taccgtcaac aaagccgctc aggtcacgct cttccaccca acggaagccg tggatctctt
    67021 ctttcacttc aatgcagtca ccaaaggctt ccatcgccgc ctgagcgaca gagaagttta
    67081 cgtcgtgacg cagagaaaga atgtggatca acacatcgaa ctgggtcgtc ggcgcaaggc
    67141 ctttaccgta gcccggaaaa tctttcagct cttctgcccc aacgccgccg ctcagagcgc
    67201 gccaggtgtt gttaccaaag gcaaccaccg caccaagatg cgcgtccggg aatttcgctt
    67261 caaaagttgc cagtttgtcg gcaaatgttt tactggccgc acgcagggcg tcaacttccc
    67321 ctttcacgtt ggcttcgatc caaatcgccg cgcggcaatg ttctggcaaa ataccactct
    67381 gaacctgaga cattgttcct cctgaaaata agaatgccac gcaatccgtg gcattgttga
    67441 gcgctatttt acgtgttttt tcgcgacggc ggtttgttca gacgcaaatt aacgccgcca
    67501 gataatttta ctgactttcc agtttttcag ggtatcgtcc gaaggcatta acccttcagg
    67561 accacgccat tccccgctga actggtagct aatatgttga ctgccttcgg ctttacattc
    67621 gacggcacga ttatcatcac catcagcttt ttggcaattg ccaaatgctt tgctgtaaag
    67681 gtcgctaaac ggtgtaccga ttttaacacc ggtgtcagca ggaatatcgc tatccagcac
    67741 gtcaatgcga ctgatcgtgc cttgatcgcc gttaatcacc atcgccacgt tgtcgccttt
    67801 catcacttca aaaaagcgca ccacgttgcc gttcgcggtt ttcattccgc tgcgcaggcg
    67861 ataatcgcca tcaagcgcat cagcaatggc ttgttcttgc agtggtgtgg acgccgttaa
    67921 ttcacccacg ccctgctcgc tcactttggt ggacgatcca aaccagttcc acgggttagc
    67981 ggcagaccag ttaactgacg acatcgtgga acagccggtc agcatcaacg gcatagcgca
    68041 taacattaaa cgcagcgatt tcatgtcact tcctttggtt attcaataac gttgcttgga
    68101 gtgcaaattc accaaaaagt gccgttattc tttttcttca ttaaaacaag cgcgtaaacg
    68161 tcgattggtc agcagccaga ttaacgccac aatatccgcc actaccagcg ccagaccgat
    68221 accgctaacg gattcaccgt tcagccacag ccacggttgc cagcaaagta aaaccacctg
    68281 cgccaacagc aacagaaaat agagcacatg ccaggtgcga ggaaacgaag cccgccgacc
    68341 gctcaacaaa aacgccagca ccgccggaat accgggaatc agccccagcc agaaattatc
    68401 gtgatcggga taaaacagat ttagcagcgc agtaccctgc tcgcgcgacg caccggcaat
    68461 gacaaacagc acccaggttc gcgcctgaag caatagcaca agccagaaga gcaagggtaa
    68521 acgcaggcgt ccgtgcgcat cataatggac aggatggaac tcagtactct tcatcttcaa
    68581 tcaaacgctt acccagactc agcacgtcgg cgtgttcata tcccaggcgt tcatacattc
    68641 cgagcaccat gtcgttatct tccggcacgt tgatctgaat tttcgggcag ccgcgagcaa
    68701 tcagcttttt ctccagccgg ttaagcaacg cattggcaat tccacgccca cgaaactctg
    68761 gatgcacgcc aagataataa gcagacccgc gatgcccgtc ataaccgccc atcaccgttc
    68821 cgaccacttc accgtttacc tcagcgacca aaaacaaact gacgtcatgg ttcatcttac
    68881 gctcgatgtc catttccgga tcgttccacg gacgcagcaa gtcgcaacgc tcccaaaggg
    68941 tgatgacctc ttcgaaatct tcctggcgaa atacgcgtat ctccatggta ttcgttacct
    69001 ttttgcgggt taaaaggctg attatggcgt gaacggtcga attagccaat atctgacgaa
    69061 aatcggttga aaaagtggca taatggggag ttgtcaacta ttgaaatgaa aagtaaaaca
    69121 attctcaaca gcaaaccgtc gtaacggatt acgcgatacg atataacatc tggaacttta
    69181 ttattacaac tcaggccgta tgagcacttt taaaccacta aaaacactca cttcgcgccg
    69241 ccaggtgctg aaagccggtt tagctgccct gacgttgtca ggaatgtcgc aagccattgc
    69301 caaagacgaa cctttaaaaa ccagcaacgg acacagcaag ccgaaagcaa aaaaatctgg
    69361 cggcaaacgt gtcgttgttc tcgatccagg ccacggcgga attgataccg gagcgatcgg
    69421 acgcaacggt tcgaaagaaa aacatgtggt gctggcgatt gctaaaaacg tccgttccat
    69481 tttgcgtaat catgggattg atgcgcgttt aacgcgttct ggcgatacgt ttatcccact
    69541 ttacgatcgc gttgaaatcg cccataaaca tggcgcagat ctgtttatgt caattcatgc
    69601 cgatggcttt accaacccga aagctgccgg tgcttcggta tttgccctct ctaaccgtgg
    69661 ggcaagtagc gcaatggcga aatacctgtc tgaacgcgaa aaccgcgccg atgaagttgc
    69721 cggtaaaaag gcgactgaca aggatcacct attgcagcaa gtactgtttg atctggtgca
    69781 aaccgatact attaaaaaca gcctgacgct tggctcgcat attctgaaaa agattaagcc
    69841 ggtgcataaa ctgcacagcc gcaacaccga acaagccgcg tttgtggtgt tgaaatcgcc
    69901 gtcggttcct tcggtgctgg tggaaacctc gtttatcacc aacccggaag aagagcggct
    69961 gttaggcacg gcggcgtttc gtcagaaaat cgccacagcg attgctgaag gcgtgatcag
    70021 ttatttccac tggttcgaca accagaaagc acattcgaaa aagcgataag ttatgaaacc
    70081 cgacgcacac caggttaaac agtttctgct caaccttcag gatacgattt gtcagcagct
    70141 gaccgccgtc gatggcgcag aatttgtcga agatagttgg cagcgcgaag ctggcggcgg
    70201 cgggcgcagt cgggtgttgc gtaatggtgg tgttttcgaa caggcgggcg tcaacttttc
    70261 tcacgtccac ggtgaggcga tgcctgcttc cgccaccgct catcgcccgg aacttgccgg
    70321 gcgcagtttc gaggcgatgg gcgtttcact ggtagtgcat ccgcataacc cgtatgttcc
    70381 caccagccac gcgaatgtgc gtttttttat tgccgaaaaa ccgggtgcag agcccgtttg
    70441 gtggtttggc ggcggcttcg atttaacccc tttctatggt tttgaagaag acgccattca
    70501 ctggcaccgc accgcccgtg acctgtgcca gccatttggt gaagacgttt atccccgtta
    70561 caaaaagtgg tgcgacgatt acttctacct caaacatcgc aacgaacagc gcggcattgg
    70621 cgggctgttc tttgatgatc tgaacacgcc agatttcgac cactgttttg cctttatgca
    70681 ggcggtaggc aaaggctaca ccgacgctta tttaccaatt gtagagcgac gtaaagcgat
    70741 ggcctacggc gagcgcgagc gcaattttca gctctaccgt cgcggtcgtt atgtcgagtt
    70801 caatctggtc tgggatcgcg gcacgctgtt tggcctgcaa actggcgggc gcaccgagtc
    70861 gatcctgatg tcaatgccgc cactggtacg ctgggagtat gattatcagc caaaagatgg
    70921 cagcccagaa gcggcgttaa gtgagtttat taaggtcagg gattgggtgt aactccctcc
    70981 cccccattcc cgcatccgct gatgcaacgt caacgacggc ttctcggcaa acagctgctg
    71041 gtaatccgtg gcaaattgcc ccagatgcca gaatccccac tgcatggcgg cgtcttttac
    71101 cgttgtgctt tgcgaccacg ggcttatcag ttcgcggcgt acggcgttca ggcgaatgcg
    71161 tttcagccac gcgtttgggc caatgcctaa aatagcgtga aacgcgtttt gtagcgtgcg
    71221 gcgactgaca tgcagttgat tacacaaatc cagcaccgtc accggttcgg acatgttttc
    71281 cagcacatat tcacgggcgc gggaaagcaa ccgacggtaa ctctgatgac tgatgctttc
    71341 cgccgtcacc attggttgcg cttcttccag catcgccccc atcgccatta gcaaattatc
    71401 ccccagcact tttcgcactg cgggctgatg gagattttcc gggttctcgc aaaacgtcgc
    71461 cagcgcctgt tggacaaagc cccacagcgc ggctttatgc tgctctttca cttccagcgc
    71521 cgactggcta cgcagcatat gtaatacccg atccggattg tgcaaaaagt tagcctgaca
    71581 ggtgatgaca tcttcagaca gcactacgcc caggatcgta taatcgtccg gcgtacttaa
    71641 ttcaaactcg gttccaccgg ggcgcgttgc tatttctgcg cttcccagac attgcgaacc
    71701 gataaatccc tgctcaccgc gcgtcgccgg aatgccaaac cagaacgagt tcggccagac
    71761 caggcacgac tgacgcagcg ccagaccggt gtattcacga aaaacctgaa tatcatcgag
    71821 tagaatttcc gtaaattcac catgaaactt gcccggatgc agctgatcgt aaatctgctg
    71881 ccaggcggta atcgtcaaag catgttcata gacatccgtt gtctgtcgtt gatgaacatt
    71941 atccacttcg accttcggcg tgagcttcag gttttcgggt aagggttcat gataaagatg
    72001 gtgcaaattg gctgtacggg tctttttcat gatgttaatg ccgggtgttg taggacaccc
    72061 ggcacctccg acaggttaat ggggcttgag acgataacga ctactgcgtt tacgttacgt
    72121 cccggcagaa aagagctgtt cgagcgcatt tctggctttt tccagcggcc agccaaagtg
    72181 ggcggccact tctcctgccg tcattccctg acatactgat gttaacagcg ccaacaactc
    72241 gtcagcagat tccgcgacga taactggcgc gtcgggttcc tttacgggtt gcttcggctg
    72301 gcggttaaag ccagtaacca gccactgggt gtcatcgtct ggccgaccga tctccttacg
    72361 gctgatgaca cgcccggtac gcatcgcggc agcacaacct gcgtccagcg ccgcacgaca
    72421 cgccgccaga tcgccttcca ccaccagcgt taagcgacca gggtcaagca cttcgtgact
    72481 gagcagacga acgttagctg ctttgagcat ggcatccgct gcatctatcg cggcgaccat
    72541 gccgtccact tccagcaatc ccagtgcatt gatcatcggc aacctccgtt acgcacgctg
    72601 gattggatta cgcgcgattt ccagcactgc atcggtaaag gcgttacagg ctgctttaca
    72661 cgcggcctgg ctaccggtta aaaatgctgc cgagtagttg gtttcagacg gtggcgggac
    72721 gtaggtcacc agctgtacgt cggcagattt caacgcggca tcaatgccgt aggtcgcttc
    72781 cagcggcggt gccaccagat acgccatcgg atcgccaagc gtgatcccgg cggttgatga
    72841 aagataagaa cctgtacgcg aaactacatg cgccaggaag gcggtgtttt ctgcgtcatt
    72901 ggcccactgg aaagctgcgc cattttcaat atgtgcaacc atcgcatcca gaccggcacg
    72961 cacttccgcc gggtttggcc caccaagcat aatcagcact tcaccggcag tcggtgacgg
    73021 gccgtgtgcc gctcccgcat acagcgagcg accatatacc acttcgacca tcgcttgttt
    73081 ggtcgcttcg tcagccgcaa tgtaggttac gtcgtcagaa tcggcagaaa tcagcccgag
    73141 gctacgaata tgcggcggca attttagttc acgcgcaaat tcagcgttaa cggaggcaat
    73201 cacccgcatg gcggttaccg acggtcgaat caaatctaaa gctggcatga tgcctcctta
    73261 tcgggtcatg ttgatgccgg atgctttctg ctccagcata cgtttcgcca aatccacaat
    73321 gacggctgcg gcttcaaccg gcggcgtgcc gccctggtga atgttagaga tacaggtgcg
    73381 atcggcctca acggtggtcg ccatacgcgg cgagtaaacg gcgtagcagg agaggctttc
    73441 tgactgccct aaccccggac gttcccccac cagcaggatc accactttcg cgccgaggat
    73501 ctcaccgatc tgatcttcaa tcttcacgcg accataacga acaaagaatg gcgtgccgac
    73561 tttcagcccg gcctgtttca ggcccgccat cagcggcggc aggatctctt cgtagttgac
    73621 ggtgatggca tcagttgaca ggccatcaga aataacgacc tgtacgtccg gattagcaac
    73681 acactgtgct ttcagcgctt caacagcttc tgcacacagg cggcggccca tatccgggcg
    73741 agtcaggtag agatttttgt cgctaatttc agagcgtact tccagcaagc cctgcgcttt
    73801 cacccactct tccggcactt ctttcagtac ggtatctttc gaacgagagt gatcggccaa
    73861 gaaacgcagc agcgcctgag tacgtggacg cggaccggca cgaccggtac aaacgcgggc
    73921 cacggtgctg cggcgcagtt ctgttaatac gtctgcgcga tgcgggtttt caacaccaat
    73981 ccacgctttt gcttcagcgg aacctaaatc cagcgcacag ctttccgagg tcaccggtgc
    74041 cgcacagttg gtggtggcgc actttgcttc tgacggcgcg ggggccgctt gtcccattga
    74101 cgccatcacg ctgcgtacaa tttcttcaat ctgtttttga tccatgatgt gttatctccg
    74161 cgtcatcaga agaacagtga cggatcgccc gcccgtttgg tcaggcgacc gtttgccata
    74221 atgcccatgc tttccagcca gcgttcaaac tctggtgacg ggcgcaggtt gagtaactga
    74281 cgcacagtgg cggtatcgtg gaatgcggtg gtctggtagt tgagcatgat gtcatcaccc
    74341 agcggcatcc ccatgatgta gttgcagcct gcggtggcga gcaggatcat caggttttcg
    74401 ttgaggttct ggtcagagtc agcgtggttg gtgtagcagc cgtcacagcc catagagatg
    74461 ccgctcagct tgcccataaa gtgatcttct aagccagcac ggataatctg gcggtcgttg
    74521 tagagatact ccggcccaat aaagccaacc acggtgttga cgataaacgg atcgtaatga
    74581 cgcgccagcc catagttacg tgcttccatc gtcacctggt cagcgccgaa gttagcgcca
    74641 gcggatagcg cagagccttg tccggtttcg aagtagaggc agttttcccc ggcgatacga
    74701 ttgaactctg cgcccactgc gcgcgcttcg tcgagcatcg ccagttccac gccaaactct
    74761 ttcagccctt tttcgctgcc acagatactc tggaaaatca gtccgccagg agcgccgcga
    74821 cgaatcgctt cgatctgggt ggtgacgtgc gccagtacgc agccctgagt tgggatgttg
    74881 aatttgtcga tcacgccata aatggtatcc agcacgcggc ttaagttttc cacgtcgtca
    74941 gttaccgggt taacgccgat caccgcatcg cccaccccga aggaaagccc ttcatagatt
    75001 tgcgcagcga tactttgcac gtcgtcacgg gtatcgttcg gctgcaaacg ggcgctaaag
    75061 gtgcccggaa taccgatggt ggtattggcc tttttgatta ccggcatttt cttcgcgccg
    75121 tagatcaggt ccgcgttgga gcaaatcttc gctatcgccg cgaccacttc cgaggtcagc
    75181 cctttgcggg taaaggcaat gtcgtccacg ctggtttcat cgctcagcac atactcacgc
    75241 agttcgctga tgctccagtt tttaatctgg ttgtaggccg tttcgttaac gtcgtcctga
    75301 atcagccgcg tcacgcagtc atcttcatag gcaatcaccg gattattgcg gatgtccgct
    75361 acggtcattt ccgacaacac ctgctttgcc gccacgcgct cctgtgagct tgccgctgca
    75421 acgcccgcca gcacatcccc cgaacgcagt tcgttggctt tagccagcac ctcttttaca
    75481 tccttaaact gatatacatt gccgaacaat gtggtcttta gtttcataag tcgttccctc
    75541 aggaagaagt gatgagtaac atttctcaca gcctgcaaac aattctattt catcgttagc
    75601 ccttgtcatg taaggtgatt tccttaacac gcccgttact tatcctgccg aacgcaaccg
    75661 aactcgccgg atacatgagc ggggttatcg agtcgttaga tcgaggtatc cggttttgag
    75721 caaaacacca ctcacagcaa aggccataga cgccgcacaa ccaaaggaca agcaatacaa
    75781 actcaccgct tcactgacgc ccggcctgtt tttgctggtg catcccaacg gcagcaaata
    75841 ctggcgattc cggtactgga tagacaagaa agagcggtta caggctattg gggtatatcc
    75901 gctgattagc ctcaaggaag cccgcaaacg cgccacagag agccgtttac tgatagccca
    75961 gggaattgac ccaatggatt tggcacgcaa ggagaaagcc attgatgcac tcaacatggc
    76021 ggcaggtttt aagacggtag cggaggactg gctggctacc agggtaggcg gctggagtga
    76081 gagctataca aagcaggtca aatcagcact ggagaaagac gtttatccgg tacttggcaa
    76141 gcgttcaatc gttgatataa ccgcccgtga cgtgctggca ttactccaga agaaagaacg
    76201 taccgcaccg gagcaggccc gcaagctacg ccagcgcatc ggtgagatct tcaagtttgc
    76261 cgttatcacc gaactggtaa cgaggaatcc ggttgcagat cttgatacgg cactgaaggc
    76321 ccgccgacct ggtcacaacg cgtggatacc gattagtgaa attccggcct tctacaaagc
    76381 ccttgagagg gccgggagcg tccagattca ggcggcaata cgtttgctta tattaacggc
    76441 attaaggacc gcagaactgc gtttgtgccg ttgggaatgg ataaacctgg aggacgcaac
    76501 aatcacattg cccgctgaag tcatgaaggc ccgccgcccg catgtggtcc cgctatcacg
    76561 gcaggcggtc gagctattgc aggaccagtt cacacgcagc ggatacagtg ttttcgtgtt
    76621 cccgggccga ttcatggata agccattatc agccagcgcg atactcaagg cgcttgagcg
    76681 tatcggatac aagtcaatag ccacaggcca cggctggcgc acaacgttca gcaccgcact
    76741 taacgaaagc ggcaggtata accctgattg gattgagatc caactagccc acgtcccgaa
    76801 gggaattagg ggagtttaca accaagcggc ctatctcaaa atgcgccgtc aaatgcttca
    76861 ggactacgcc gacgcgattg attcgatact ggctgatggt atcaatccgc ttgaactgta
    76921 atgaatgatt aatcacgttt tccgacgact ccaccggagt aggccggata aacactggac
    76981 accttcaaaa taactattca ctttttccgg attcgtccgg ctttttttgt ctttgccgat
    77041 gggatcaatt attccaatta ttccagtatc gttggaataa atgaataaat atcaatgact
    77101 taaagccgtg tttgcaacag ggtggatcgg ggatcgaaaa taggatcaac acaaaaacac
    77161 cctggaggct acagcacact agggggacac aaaaacaaca caggaaaaaa ataccatgta
    77221 ccaatcagca accgcctacg tggcgaaacc aggcgaaaca ctggtcaaac acaaaattgc
    77281 aacactccaa gcacttcaaa cactccaagc ccttccggaa caggttcagg ccgcctttcg
    77341 tgatggcatg aacatcatcc gcttcgatgg gtatcgctgg gaatacaagc cagcaactgg
    77401 attcgtgcgc atagatcaaa tcttgtcgcc agccaatgaa gagccagcgc tagccaatgc
    77461 agaaccagcg caggagcagg agcaggagca ggagcaggag caggaacagg aacaggaaca
    77521 ggaacaggaa caggaacagg aacaggaaca ggaacaggaa cagaataaag cggcggctat
    77581 tgccgagttc aaggagctgc aaagacaaca aatcacagcg cagcaagaag aatgccgcca
    77641 cccgctggca atgcccaagc cagtagcgaa agccaacaca gacaagccga cttcatggtt
    77701 taacgaagaa gcggaagaag atttgcgttt tgatagtaaa gaggagcagt tgcctgaaga
    77761 gcgggaacag gtggaaacgg aagaccagca aaacgattat cgcaaccttc cagccgtcaa
    77821 taacgctatg gcttacctct tcaaaagcgg gctgtttcac aagctgaaag gcgatgagtt
    77881 aaagctatat acgtggatgg tatccaagtg caggtatcgc ggtaacgaca aatatgagat
    77941 ttggtattcc cgcaccaaaa tggcaaaagc gttaggtaag agcgttgaca cgatagatcg
    78001 ccacctggaa gcgttgagaa agctgggact tatccaggaa atgcgccagg cactgagggg
    78061 caaatgtgga aaaacctaca gactgcgaat cccgtcaaaa ggcacagcag aagaggcctc
    78121 aatgacggca cagcagcacg aaagagaagc aaaagtcagc tacctggtac actaggcagc
    78181 gggtcagcaa aggggggcaa atgccccttt tttcatgtca gataatcaga tcattacctc
    78241 aataaactaa taatcacatc gcggttacaa ccagtttgat agtgatagct caaacccaga
    78301 cgtggcgctg gctgcaagca aaataagccg caaaaatgcg gtcctatttg ccagaacctt
    78361 ttattattca attggttagt gcaagaaaaa gcgtgattac tgttcaagaa gcatccagaa
    78421 aggtcatgat tttgccatga ttttgacgct gaaaaaaaat aggtgggcat ttttgcccac
    78481 tcactagccg caaatttgcc cactcactag ccgcattttt gcggcccaaa gtaataaata
    78541 cagagaaata ataaatacag atcagcgcca agatttttcg tcttttcggc ggaatcatga
    78601 gactagtgct tacgtcctga taacctccag agcagacctt aacttctgtg cagcaggact
    78661 acgctcattg ccgctcggct aggtcaaatc ctcacgataa agctgttcgg attgtgacca
    78721 taatgttaaa ggactcttta aatcattcct tgacgaatta actagctggc ttaagcggaa
    78781 tagcatgcag gataaaaaca aaaccagctt agcagtgggg agagcttcgc ttcgctcgtc
    78841 tcctggggcc aatgctcaat gaaacgacgt aaggcacgga gcgctcacca taggtagaca
    78901 cataaacatc tgagagcgca ccttctatcg cgctacgcgc tcaataaaaa ccaacgacac
    78961 cgccacactg caagaccaat ggtttttatc gcgtatacag accagacagg aaacagcagc
    79021 aaagcagcgt atagccaggt gcagacagca cactcacacg acgcacagca ggcttaagtg
    79081 atcactcagg aaactttatc cccgccgccg atcaccgcga actatcatga ccgcgccaaa
    79141 ttcgaaagga atggcccata gaacgcgatg aacacacata agcgctaact tgcttacctg
    79201 aaaacttaac gtcgccacag agcgattgag agcgcttaaa atcgatgcga tttcattaaa
    79261 tttttctcac tcgcctgaat ggtggcgatc aggactgatc gcaacattgc cccgcctcga
    79321 tattgagcgc ataatagata catacattaa gcagaaaaga gataagttta ttgtgaaaat
    79381 cagggggtta cacatcatgc gcatatcaga acaacaacgg cagatcctcg actatctggc
    79441 aaaaaacgaa tcaatcaccg cacgacaagc cgcagatctc gtttatggtg gcaatgttac
    79501 ccgcgtccag gttgacgccg cccgccgctc actcatgacg atggttagta atgggctaat
    79561 caggaaacaa ggccgcgctt tcgtagctga aagacatatc aatcacgctt atagccagga
    79621 cgttgaagaa ttgcgccgcc agatcctttg ggtattcagc aaacagggtc aggagtattt
    79681 cctttctgaa cacccaaatt tcgccgccaa agagttaaca gtaagcggat tgtcgtgcgc
    79741 actggattat cttgtcggtg aaggttacgt tacttacaag cagctatggc acgccctgaa
    79801 tacgcttgta gaagatggtt tactcattgc cacgcgtgag aacatcgaat actggataat
    79861 gccgcgttca ggtcgccgca atcatgcacg gtatatttca ggcaacagat acagaacgcc
    79921 ggactttaat ccgacacagg aagatcggga ccgtgccgaa atgatgtgca gggaatagga
    79981 gaaaagaatt atggataagt tattttcgat gcagtgaaca caagcgggcg caatgcccgt
    80041 ttttattgcc ttattccacg cctgacgaat caatttgatc acacctgagc aaatgcatag
    80101 ccagaagtta aggccgttat attgcgattg ggagcgtttt aaacggtatc aatttaatta
    80161 attctgatgt ggtgatttgc aggcaaaaaa aacccgccgg agaagcggga aatagagaca
    80221 tatcgttgta gttattaaca gatcactttg aggtcgaggg cggctaaccc tcgctaatat
    80281 tatcccgcca ttaccttgcg ggtgcaaata atcttttatt gctttgatga tttaataaat
    80341 agcacttttt gctaacaaaa accgctgtca atatatggcc aaaaatgtca acagaaaaaa
    80401 taaggtttat aatattaagt atcacatggg taatgggttc agggaatcgg aatccaccgc
    80461 ctcacggaaa ttcaaaattt gcttcttctt tcctccgtgg ggcggcttct ttttcgtatc
    80521 gaggtattta tgaaagcaat aacattagaa cagttcgcag caatggcgaa tgtatcgacc
    80581 agaacgctga ggcgctggct aaaaaaacca tcaaccggag tccctttgcc atttacccca
    80641 cgcggtactc gccaattatt ctgggagcac gaatgcaaag aatggttgta cagaacagaa
    80701 cgataataat cacgcatcac attttgaggt aaacatcatg accaatatcg aaagattaga
    80761 acgctgtgcc gatattatgc ggcgcagatg gatttatgac cctgaagcgg gtttattgtt
    80821 tagccgtgaa acaaaagacg ttatcaaagg cagcatgacc aataaagggc atttggttgt
    80881 aacggtacat gaaggtaaat tcaaagtgac actcacctat cagaaagcct gctatgtata
    80941 cacatacggc gcttatgacg agacattata tgaagtgcac cacgttaatt tggacaaaca
    81001 ggataaccgc atcaagaata ttcgcctgat gccgaaagaa aagcaccgcc ggatacataa
    81061 aaacgtccgc aaacttattc ggctgggcca cacacaatgg cttcaaccgc aattaattga
    81121 acaatcacta taaacacagt agtaaatacc atgcattcat tagacagtta ttttcagcgc
    81181 acgacagccc ctaaatcagc cgcccaggaa cgccgtgagg aattccatga gaaagtgatg
    81241 cgttctgctg attatattgc ggacaagttc gtggaaacgg ttcgtccact tgttgatgaa
    81301 gtagctgata aacttcagtc tgaaatgccg gaagatatgg aaggcactgc aaagcggcga
    81361 ctcatttgct aattaagtcg tcgttttggc gtgtcaataa gcgcttttaa ataatcgaaa
    81421 aaagggggga aagataatgg acgcatttga ggtactggca aataaaggcg ctgaactggt
    81481 agcacagcgt gataaaaccg ccaacgaagg ggaacgcgca ctgctaaata aacagattaa
    81541 agcgatccgt atggcccagt ttaaattaat cagtaatgaa gttatcgaaa caattaaacc
    81601 gcagatccca caaatcgttg ctgacgctat taaagccgcc ggactggaga aacgcattgc
    81661 cgggctaaaa acgaacgcag agaaaatgga gatgcacaaa cagagcggtg ctccgaatcc
    81721 cgatgagtac ttctcaccgg atgttgagtt tatggcgcag gttgaggaac gtctgggtag
    81781 ctgtctcacc gaggaacagc gtcgctattt tgatggagtg gatagttcgg caggtattga
    81841 cctgaactcg tatttcggtc gtgaaattga gcattttgat gccgccccgt ctatgccgga
    81901 gccagaacca gaaatggcca ccgatcctga agctgagcaa cggaaacgcg tattcaatgc
    81961 gatagggatt cggtactgat cggataaaag aaagccggga attaccccgg ctgacttaac
    82021 aatgtgacca atgaacaaat cggacaagac aaacaggata agcagtctcg aaacaatgtt
    82081 cgggaactat attagcacaa gtccggagaa aatcatcatg ttggcactac gcatcatgca
    82141 gggcatcgct aagaccctcg cagaacatgt cttagacctg aaacattcgc cactgagcaa
    82201 acaggccatg aagcgctaaa cgcttcgtct gtgggctgaa tattcgcttg gaactatcaa
    82261 taaaatcatc gacatgaaat cggggccgag caaccagagc gcggaggaaa tggagttcat
    82321 caagcgttta attctcattc gcagagacat tcatagccag ttacacagcg tgggaatcga
    82381 tattaatgat gggacgggcg attaattttc ggacaagtac tatgcgcgcg cgaggagctg
    82441 aacatgaagc aagatattgc agacaggctg gagatactcg aaggccagcg ggcagaagcg
    82501 aaacaactac gtaagcaggc cagaagagcg cacaggaaca acgaagcaga gttactaacc
    82561 aaatacatca gtttcaccaa ttactgcatc tacgagtgtt acaaggaaga tgcagaggac
    82621 tggctggatt cattgccaga acagtactga gcagaataac accacgtcat gttgttctga
    82681 ggtgagcata tcacctctgt tcaggtggcc aaatttagtg tgccacttca gcatcaccgt
    82741 tgaaatcaca ataacgattt ttttgtaaat atgctctcac atatgtagga atagaccagg
    82801 ttggcattga aacacatcta ccatgaccat aataaagtaa ttttttgata tgtttatggt
    82861 tttatattaa ttaaaatata tctaaaattg atagttttaa aattgcaaaa tccagattgt
    82921 gtttgtaatg tttaatctga ttgtattgag tggcgtttat tatgaaaaat aaatcaggta
    82981 attcttgtta tagagaagca gtcgatattc atagctcact tgagctttca tttatattgc
    83041 ataaatatca tttgcgtgta gatgagaaga aatctcgaaa tgtattcagc gccatacgtg
    83101 atgagctcat ccttgatggg gattcacgac aaaacttagc tactttctgt caaacatggc
    83161 ttgatgatga aatccgtgag ttgcttaatt tagctattga taaaaacatg atttataaag
    83221 atgaatatcc acaaatggcg gaacctgaag gtcgctgtgt gcgtatgctt tccgattcgt
    83281 ggagtttccc cgattcgcga cacaccttag gttgctcaac cattggttca tctgaagctg
    83341 ctatgctcgg ccgcttggcg ttaaaatggc agtggtgtaa aaaacgcgaa gtacaaggta
    83401 aatcaaccga acctgatttg cgatcctgtg caaatatgct ggcataaatt ctgccgttac
    83461 tgtgatgtga aattgtgtga aattccactt gatgatcatc acctgataat gaatgctaaa
    83521 gaggttttga agcatgtcga tgaaaacact attggtgtcg ttacgacttt aggtattact
    83581 tatttttgta aagcagattg cgttagtgtt tttaagataa gtgttaaagt taatattagt
    83641 ttttatattt acatcgtaat tatatgtttc tattttaaaa atattcgaga gtataaaaat
    83701 gtctaatgct aatatcgcac ccaaaagtaa gaaaatgaca gtggggacac tcgctatcat
    83761 gaatataact gctgtagtaa gtctacgatt tataccttct gaagcagaat acggcttagg
    83821 tgccattttt tactatacct ttgctgcaat tatgttttta gtacctgttg cctttgttgc
    83881 ggctgagcta gctacaacat atccacaaaa agggggggca tttcgatgga taggagaagc
    83941 atttggggct cgctggggtt ttgtaggaat gcttatgacc tggctacaag tcatccccta
    84001 ctttccaaca gtactgacat ttggtgcagt ctctgttgca tttattgatg ttgatatggg
    84061 ggtggcagaa tcaattgcca gtaataaatg gtatatttta tttttcgtac tatttgttta
    84121 ctggggagct atttgtgttg cattacgtgg cgttggtatt ttctcgcagg tatctaagtg
    84181 gtgcggtatt atagggacaa ttattccagc tattatcctt gttattcttg gtttctccta
    84241 tctgtggttc agcggtaaac cagcggtgat cccattaggt tggggtgatc ttttccctaa
    84301 tatagctaac ttccagaata ttgttcttgc cgccagcata tttcttgctt atgcagggat
    84361 ggaaatgaat gcggtccatg ttaacgattt agataatcca actaaaaaat acccaattgc
    84421 aattaccatc gcttctatag gtactattgc aatattcctg ctcagtactt taggggtcgc
    84481 ctttattatt cctacagata aaattagtct aactcaaagt ttgttggttg cctacgatat
    84541 gctgttcgaa tgggctggag tgccatggct tggctctgtg atggcattta tgctagcaat
    84601 tggcgtactg ggtggggtgg ttacttggat tgcagggcct aatacaggtg tattagcgat
    84661 cgcgaaagct ggttatttac ctaagttttt ccaaaaaacc aatcgtcatg gaatggggca
    84721 ccatctaatg tttgtacaag gtataattgt ctcagttctt tctgttactt ttgtgataat
    84781 gccttcagtt caagcagctt ttcagatttt atcacagtta acagttatgc tctatttagt
    84841 tatgtatatg ctcatgttcg ctagtgctat ctatctacga cattctcaac ctgatacaaa
    84901 acgcccatat cgagcccctg ctttattttt ctgggcaaca cttggattct tagggtcttt
    84961 tctggctttt gtattaagtt ttattccgcc cgagcaaatc ccaactggta gtccaaccag
    85021 ctatgttctt tatttgttag tattagttgc cctattttta ttaattccaa tattaattta
    85081 cgccttccga aaatctgact ggaaagatcc aaactctgat ttcgaaccat tttcttggga
    85141 aaaaagaact agtaattaaa ttttccataa gctacaaaag atgaggttaa atatgtttag
    85201 tgctgaatct ccaattggtg taatagactc aggtgtaggt ggattgacat cggtaaagga
    85261 aataattaat ttgcttcctg gtgaagatat tatctattgt ggagataata tgaatgcacc
    85321 ttatggcaac cgttctgccg atgatatcat cagtttgact aaaaaaatgc tgactttttt
    85381 acagtcaagg aatgtcaaac tagttgctgt cgcatgtaat actatatcct caactttgga
    85441 atccgaaaaa tacagcggat atgctaagag ctttccgttt cctatcttat ccatcattga
    85501 acctgcagta gaagatgtga ttcgccagca atataaaaat gttggtatta tcgctaccga
    85561 gtttactatt aaaacggggt gtcacaaaga gctaataaaa aaattgaatt caacaataaa
    85621 tgtatttgga gaaccaagca aaaatcttgc aatgttaatt gaggaaggta atcttaatgc
    85681 tcctgcaatc ttgaatgaca tcaaaaaaca tgttaaccat ttaatttccc tccatccagt
    85741 aaatgaaatt attctcggct gtactcacta tccaattgtt cagaattttt ttgaagcagt
    85801 tgcaccggat ataaaattta ttaatcctgc acatgatcag gctatctcta ttaaaaatca
    85861 cttgggacag ctaaatttat taaactcctc aaatatagga actttgcaaa taaatactag
    85921 tggaagtatg gagatctaca aaacggtact aggggaattg agcattacga aacctcatac
    85981 attcagtatc aggcagtttt aaatttatcc ggtgcaccgt aaaatcccgt ccttctgaac
    86041 tgcaccccaa aagttggaca ccaactgagt aaggtgcatt ttatggacaa acccagttac
    86101 atactgggta gtgcatccaa ttagtagaac atgtgttttt cgataaacgc tccgatcact
    86161 ttttcgtgga tctcaactga gcgtgagaag cagattgttt tacgacccaa ccgcttaatg
    86221 cgggtgcgta gcgtcagatt attacgctca atgcgttggg tgaatatttt gccggtcaga
    86281 tgcttattct tcggcacctc ccggccatag ctgccccagt catcgctggt cagcatgccg
    86341 atgttgaagg gtggcagtgc cagtagctcc cggcacgttt gatcggttcg gggaccaaaa
    86401 gtgtaggcca gtacaccgcc tgttttggtg ttgtacgcgt acccgagcca atgttgccgg
    86461 gctttactgc caacgtagct ccattgctca tcaagctcgc agataaacgc cacatcagca
    86521 tgggcaacag gcgaagaagt tattcgcttt ggcgcgagtt ttttaaagtc cggatgacgg
    86581 tgttactacc aattttcagt gtcctggcgg tatcgcgaac cccggcacca ttaaaggcca
    86641 tttcagttat cagctctttc atacccggct tacgtgcttg ataagtataa gtgaactgaa
    86701 acacacggtg gcagtcacgg cagcgaagtc tgtcacggcc tttagggttc tgaccatggc
    86761 ggtaaacctg agctgactga caacggggac aatgaatatt aacgctggtc atgagataac
    86821 ctcaaaagcc cgtattatac atcagattca actaattaga ggcatcaccc atccagcatt
    86881 ataaaacata ctaacaataa ctaaattata ataatcacaa cttacaatca aataataaat
    86941 gcataagatt aacaatgatt ttttcgggtt atatttatct aaaaaagcag aatattatgt
    87001 aacttattat tttctaagcg aaaatttatt gtgcagtcag actagcaaac ttgcgtacca
    87061 cccgaagaac atgcgaagat ttaagtattc tttctttttt ggtttttatt tttcattttc
    87121 tcttttcttc tctttccggt tctcttctgt tctggttttg gttttttcct ttttattttt
    87181 attctttctt cagggttatg ctaaaacacc aaaacatttt tcaccacaaa agcatccaat
    87241 catcttgcca gaccaatcac cttaaccgga tacactgccg gatataatcc cactaacacc
    87301 cagccaccaa gcaccatacc tgataactac cgtattcagg aaggaaatgc gagtgatttc
    87361 accgtcaccg gcacaaccga tccgccaaaa agaggcgtac caatgtcgat atagtccccc
    87421 gcgcggacaa tcacttcgtc aatgactgcc aacgggagtt gttgtagctg tgggcgcaac
    87481 aacatgccca gagctttacc aaagtcctgc ccggccacca ccagcagggg atgcggattc
    87541 ggaaaacgcg cgacgaaatc gaccagcgcg ttgatgaccg tcagtaccgc ggcgtaacgc
    87601 acaggcagcg aggcgggaag cgccagcacg tacacgtcag ttttgggatc aagatccagc
    87661 tgaagcagcg cctgttgcca ggcactcacc agatccgttt catcaatcgg gatcgccacc
    87721 ggcaaattgc gcagcggcag ttgtacgccc tccagccaga ttgtgctgcc agagagcgaa
    87781 agggtatgtg cacccgcgcc aatcaccgtg gcgcatacgg tttgcgccgg aaactgcaca
    87841 ttcatctcac gcaggcgcgg atggtcatgc agcgccgttg ccagaagtgg gccaatatcg
    87901 gcaaaacaga atgggtcggc gggctggtgg cgataacatt cgcccacacc tccagaaagc
    87961 gtaatgattt cgggcgtaac acctgcgggc agcaaaccgg tttgcatcaa tgcctgcgcg
    88021 agcggcgaaa gcgttccgtc aatcacttcg acaatcagcg ccgccatccg ccgggtaacc
    88081 tgcaccagct gcgcgccggt cagcgaacgg gcgtcagtgc ctgcaccgaa gcactcatcc
    88141 acaatcatct gccccggttt atgagcgtaa accacgcgcc cctggctgtc ggtttccagc
    88201 aggcgaccac cgacgttgag acaggcagtg ccgctgattt ttccggcatc gaacagggcg
    88261 tagttcgcgg tgccaccgcc gatatcgata ttcagtaccc gacacagccg ttgttcagaa
    88321 agggtttgcg ccccggctcc gtgaccggcg atcacggatt cgaggtgcgg cccggcgctg
    88381 gcgacgacaa aatcgcccag cgactgtttt tccttcatca ctttttccat cagctgtgcg
    88441 accaggcttt cggtaaactg gccttccggc agctgggcaa tgatggtttc gcggatgcgc
    88501 tggctttcgg ttttgtcggc tggcacgctg gcgggcacgg acgccgttac cgggatcgat
    88561 tcatcacatt cggtgatggt aaaacccagt agatccgaca cttcacgggc ttccggggta
    88621 ataatgctgg cgcgcagaac cacggacatt gcctgttcgc cgcgcgcgtg tgcttcacga
    88681 atatcattcg ctgtgatgag ttttttcacc tgcttcctcc tgtttggtga gagatgccag
    88741 ataatccacc agttgctgca cgctttgcgg gtcgtggctg tttagctcga aaatcggctc
    88801 ttcgaaaccc gtctcaagca ataactttcg cgttgcggcg acgtcagcat ctggcatgtc
    88861 cgttttactg atgacggcga tttgccgttt gctgacgcca atatccagca acccggcagg
    88921 caaccgactt tccgggtcat ttgcgccatg gacatagatc agcatgtcta catcctgtag
    88981 cgtggtaatt aaggcgtgat accagcgggg atggctaaaa tattcacccg gcgtatcaat
    89041 atcgccctta tcattaaatt ccacggcctg tgtttttctg gcgagggtat aattcccctg
    89101 caacgcatta aataacgttg tttttcctgc cccgacgcta cccacaaacg caattcgttt
    89161 catgaatgcc tcgtattaac ttttggtcat ttcacaaagc gtataattta ataagcgacc
    89221 taaaccgctg acggtttgtg ataacgcttc ttctactgcg cccaccgagc cataaatcac
    89281 caatgcaccg ctgaagcggt cgagaaaacc gatatgcaca tcagcggctt tcagcgctaa
    89341 atcaccggcg atcatcgccg tttcgccggg agttagcgtc ataatgccaa ttgcgcccgc
    89401 atccggaacg ccgattttct tcgccagttc ctcaccggga tgcgcgatga gatgcgccag
    89461 tgtgacctgt ttgccgggca caaattcctg aatgatgcgt tctttatcca tgagtcgccg
    89521 cctgtgaaaa actttcgtgt atcgtgacaa tttttcgtca gagtaaataa caaaattcgg
    89581 caagcgcgtt taaaaggtga ggtagatcac taagaatgga gagatattta ctgttatgaa
    89641 taaactttca tatgcgacgc atgaatataa ataaaataat atcgagatat tttatatact
    89701 gattttttta ttattcgcaa actcagtaat aagctttgct aatatatttc aggaaaaccg
    89761 cttaccctga atattcaggg taagcgtgag agttaagaat gaattacagc ggttgggttt
    89821 gcgcttcaac cacggccagc gccaccatat tgacgatacg acgcaccgat gcgatcggcg
    89881 ttaacacgtg aaccggtttc gccacgccca tcagcaccgg accgacagtc acaccttccg
    89941 agctggaaac gcgcagtaag ttgtaactaa tgcgggcagc ttccatgttc ggcatcacca
    90001 gaatattggc agaacctttc aaagggctgt ccggcatacg gtcgttgcga atcgcttcca
    90061 ccagcgctgc atcgccgtgc atttcaccat caatcatcag atctggcgca cgatccctga
    90121 ccagttccag cgcctgacgc attttgctcg acgacgggca gtcagaagaa ccaaagttgg
    90181 agtgcgacaa cagagcaacg cgcggctcaa taccaaaacg acggacagtt tctgccgcca
    90241 tcaaggtgat ctccgccagc tcttctgcgt ctggttcgtc attgacgtag gtatcggcaa
    90301 caaaggtgtt accactcggc agcagcagcg cgttcattgc acctgcggtg tgaacgccat
    90361 cgcgataacc aaagacattt ttcaccacgc taaaatgttc atgataatcg cccaccgtac
    90421 cgcagatcat tgcatcggct tccccacgct gaaccatgat cgcgccgatc actgtcgggt
    90481 tactgatcag cgcacgctgc gcctgttcct gagtgacacc gcgacgcttc atgatctgga
    90541 agtattcggt ccagtactct ttaaagcgcg gatcggattc gttattgacg atttcaaaat
    90601 caacgcccgc tttgatctgc aagcccagtt tctgaatgcg catttcgatc acgttcggac
    90661 gaccgataag gatcggtttc gccagtccca gcgttaccag ttcctgagtc gcatgcagaa
    90721 cgcgcgcctc ttccccttcc ggcagaacta cgcgcttcgg cgctttgcga gcctgggaga
    90781 aaatcggctt cataaacagg ttggttttgt aaacgaactc agtcagcttg tcgatgtaga
    90841 cgtcgaaatc agcaatcgga cgcgtcgcca cgcccgactc catcgccgct tttgcgaccg
    90901 caggagcgat cttaacgatc aagcgcggat caaacggttt tggaatgatg tattccggac
    90961 caaagctcag atcctgaacg ccatacgctg aagccaccac ttcactctgt tctgcatggg
    91021 ccagttctgc aatcgcgcgt accgccgcca gtttcatctc ttcgttgatg gcggttgcgc
    91081 caacgtcaag cgcgccacgg aagatgaacg ggaagcaaag aacgttgttc acctggttcg
    91141 ggtagtcgga acggccggta cagatgatgg catccggacg cacttctttc gccagcggcg
    91201 gcagaatttc cggttccggg ttcgccagcg ccaggatcat tggtgcccga gccattttct
    91261 tcaccatttc ctgggtcagc actttcgggc cggaacagcc caggaaaata tccgcacctt
    91321 caatcacatc atcgagggta cgtttgccgt catccaccac cgcatatgcg gctttggttt
    91381 ccgccatatt tggctcacgc ccctgataga taacgccttt tgaatcgcaa accacgatgt
    91441 tatgtttttg cagccccagc gctaccagca ggttcataca ggcgattgct gcggcacccg
    91501 cgccggaaac caccatccgc acgtcggaga tgtttttctc caccacgcgc aagccgttga
    91561 ggatggcggc agtgctgatg attgccgtgc cgtgctgatc gtcgtggaat accggaatat
    91621 tcatccgctc gcgcagtttc tgttcaatat agaaacattc tggcgcttta atgtcttcga
    91681 ggttgatgcc gccgaaggtc ggttcgagcg cggcgacgac ttcaataaat ttgtccgggt
    91741 cgagttcatc aacttcaatg tcaaatacat caatcccagc gaatttctta aatagaacgc
    91801 ccttgccttc catcaccggt ttgcctgcca gcgcgccaat gttgcctaac cccagcaccg
    91861 ccgtaccgtt agagatcacc gcaaccaggt taccgcgcgc ggtatatttg taggctttta
    91921 gcgggtcttt ttcgatttca agacaaggtg cggcaacgcc tggtgagtag gccagcgcca
    91981 gatcgcgctg cgttgccaga ggcttggttg gagaaacctg gattttccct ggaactggaa
    92041 attcatggaa atcaagtgca ctttgtttta actggtcatc catttggttg ttcctttcac
    92101 gtaacgttca caaataaagt gtgtgggcaa cagcccctgc ccacaacgtg gcgcacatta
    92161 ttaccctgcc ggagtctaca gactttgagc aagtccaaac tctcaccatt aatataatgt
    92221 tttggtaaca atcctataac actgatatta cctgcttaat ccagcaatac catgcctgtc
    92281 tgctatgctt ttttgatgcg tttagcgaaa tttctcagaa gtgtgaatta acgcactcat
    92341 ctaacacttt acttttcaag gagtatttcc tatgaacgag ttagacggca tcaaacagtt
    92401 caccactgtc gtggcagaca gcggcgatat tgagtccatt cgccattatc atcccctgga
    92461 tgccaccacc aatccttcgc tgttactcaa ggctgccgga ttatcacaat atgagcattt
    92521 aatagacgat gctatcgcct ggggtaaaaa aaatggcaag acccaggaac aacaggtggt
    92581 cgcagcgtgt gacaaactgg cggtcaattt cggtgctgaa attctcaaaa tcgtacccgg
    92641 ttgcgtgtca acagaagttg atgcacgcct ctcttttgat aaagaaaaga gtattgagaa
    92701 ggcgcgccat ctggtggact tgtatcagca acaaggcgtt gagaaatcac gcattttgat
    92761 caaactggct tcgacctggg aaggaattcg cgcggccgaa gagctggaaa aagaaggtat
    92821 taactgcaac ctgacgctgc tgttttcttt tgcccaggcg cgggcctgtg cggaagcagg
    92881 cgtttttctg atttcgccgt ttgtagggcg tatttatgac tggtatcagg cacgcaagcc
    92941 gatggacccg tatgtggtgg aagaagatcc gggcgttaaa tcggtgcgca atatctacga
    93001 ctactataag caacaccact atgaaaccat tgtgatgggc gcgagcttcc gtcgcaccga
    93061 acaaatcctc gccttaaccg gctgcgatcg actgactatc gcaccgaatt tgctgaagga
    93121 gctgcaggaa aaagtttctc cagtggtacg taaattaatc ccgccttctc agacgttccc
    93181 acgcccagca cccatgagcg aagcggagtt ccgttgggag cacaatcagg atgcgatggc
    93241 ggtagaaaaa ctgtctgaag gcattcgtct gttcgctgtt gatcaacgca aactggaaga
    93301 tcttcttgcc gccaaactat aaaccagcca cggagtgtta tatgtcccga aaagaccttg
    93361 ccaatgcgat tcgcgcactc agtatggatg cggtacaaaa agccaattct ggtcatcccg
    93421 gcgcgccgat gggcatggct gatattgccg aagtgctgtg gaacgatttt cttaaacata
    93481 atcctaccga cccaacctgg tatgatcgcg accgctttat tctttccaac ggtcacgcgt
    93541 cgatgctgct ctacagtttg ctacatctga ccggttacga cctgccgctg gaagaactga
    93601 agaacttccg tcagttgcat tcgaaaaccc caggccaccc ggagattggc tatacgccag
    93661 gcgttgaaac caccaccggc ccgcttggac aaggtttggc gaacgccgtc gggctggcga
    93721 tagcagagcg tacactggcg gcgcagttta accagccaga ccatgagatc gtcgatcact
    93781 tcacctatgt gtttatgggc gacggctgcc tgatggaagg tatttcccac gaagtctgtt
    93841 cgctggcagg cacgctggga ctaggcaagc tgattggttt ttacgatcac aacggtattt
    93901 ccatcgacgg tgaaacagaa ggctggttta ccgacgatac ggcaaaacgt tttgaagcct
    93961 atcactggca tgtgatccat gaaatcgacg gtcacgatcc gcaggcggtg aaggaagcga
    94021 tccttgaagc gcaaagcgtg aaagataagc cgtcgctgat tatctgccgt acggtgattg
    94081 gctttggttc gccgaataaa gcaggtaagg aagaggcgca cggcgcacca ctgggggaag
    94141 aagaagtggc gctggcacgg caaaaactgg gctggcacca tccgccattt gagatcccta
    94201 aagagattta tcacgcctgg gatgcccgtg aaaaaggcga aaaagcgcag cagagctgga
    94261 atgagaagtt tgccgcctat aaaaaggctc atccgcaact ggcagaagag tttacccgac
    94321 ggatgagcgg tggtttaccg aaggactggg agaaaacgac tcagaaatat atcaatgagt
    94381 tacaggcaaa tccggcgaaa atcgctaccc gtaaggcttc gcaaaatacg cttaacgctt
    94441 acgggccgat gctgcctgag ttgctcggcg gttcggcgga tctggctccc agcaacctga
    94501 ccatctggaa aggttctgtt tcgctgaagg aagatccggc gggcaactac attcactacg
    94561 gggtgcgtga atttggcatg accgctatcg ccaacggcat cgcgcaccac ggcggctttg
    94621 tgccgtatac cgcgacgttc ctgatgtttg ttgaatacgc ccgtaacgcc gcgcggatgg
    94681 cggcactgat gaaagcgcgg cagattatgg tttataccca cgactcaatt ggcctgggcg
    94741 aagatggtcc gacgcaccag gctgttgagc aactggccag cctgcgctta acgccaaatt
    94801 tcagcacctg gcgaccgtgc gatcaggtgg aagcggcggt gggctggaag ctggcggttg
    94861 agcgccacaa cggaccgacg gcactgatcc tctcaaggca gaatctggcc caggtggaac
    94921 gtacgccgga tcaggttaaa gagattgctc gtggcggtta tgtgctgaaa gacagcggcg
    94981 gtaagccaga tattattctg attgccaccg gttcagagat ggaaattacc ctgcaagcgg
    95041 cagagaaatt agcaggagaa ggtcgcaatg tacgcgtagt ttccctgccc tcgaccgata
    95101 ttttcgacgc ccaggatgag gaatatcggg agtcggtgtt gccttctaac gttgcggctc
    95161 gcgtggcggt ggaagcaggt attgccgatt actggtacaa gtatgttggt ctgaaagggg
    95221 caattgtcgg gatgacgggt tacggggaat ctgctccggc ggataagctg ttcccgttct
    95281 ttggctttac cgccgagaat attgtggcaa aagcgcataa ggtgctggga gtgaaaggtg
    95341 cctgatggtg atcgccggat gctgtatgtt cccctcaccc taaccctctc cccagaaggg
    95401 cgaggggacg gtccgtgcgc tttttagggc cgcatctgcg attttgttta acgggtgatc
    95461 acaacgtggg cctggcgtct ggcccatgcc agttatcgca ggtaggttct gcggcgtaac
    95521 gcaccaggcg aaaactctga ccgtcaaagc gccagcgcgc ctgaatgcca caatcgctta
    95581 atccgcgccc tttcgctaag gtcaccagtt cacgcgattt ctcatcaaat gttgcgttca
    95641 tcagttccag ttcattcgtc tcctgaccgt tgttgaacgg caaacgcaac cgaaccgggc
    95701 gcgaggctag cggcttttta cgcgacacaa tccatgccaa atcaatggtg ttataggccc
    95761 ctgcctcaca gctaatcatc atcagtgctt tatcatcggt cagcgcagtg acattcacct
    95821 cacgacgtaa cggatcaagc gagcagcgca gaccattcat ccgccagttt ccataatcca
    95881 gcaaatcgtt acgttcttcg agtgagagtg gcgtcggcgt tgggttaacc accgcgacct
    95941 ctttcagcgc aggcgcgggc ggtacgctga gaggcggttc gtcccctttc ttgatccacg
    96001 cggtttcact gccaacgcgc ttttgctgag catcaataaa caacaacgcc gcttttaagc
    96061 cgctcagaga aatggtctga ttgccgtcgc gtaaggtgat cgccttccct tcctgaatta
    96121 tctgcaaaaa cgcggtgatg gttgccgtat catcggttac taataaccat ggtgaaatcc
    96181 gccacttgtc accacttaat gccaacggct caccatctaa tagcagccgt ggcgctatct
    96241 ccccttcaga cgcatccggc gacttcaacc caccgcgctc aatacgtaaa acggcatcgg
    96301 tatgcgcccc agcgctgcgg ctcagggtca tcaccagtcc attatgatcg cccgtattgc
    96361 gcgccacaca aaaattttga ttattacagg tgacctgcca gtcggaaaac gcccgttgcg
    96421 ctggtgccgc ccacaccaaa gacgtcggca acaaagcgaa aaaaaagaga aggaaaatgc
    96481 gatagcgcat ggacggtacg accccagaag ttcaaacaaa cagcacaagt cgtatcttcg
    96541 ttgcgcaggt agcgcggctc aatcggattt gtcggattga gccaataata aacaacgaaa
    96601 tattattgct atttttcagt ccattaaatg tgacatttgc aaatagttaa gcaataacac
    96661 cgtcttaccg tcacgtatct cgccggtttt gatcatctcc agcgcctggc tgaacggcag
    96721 ctcgagcact tcaatatctt catcttcaac accgccaccg gcgttagcgc gctgattgtc
    96781 actgtattcg gcgataaaaa agtggattag ctcagtcaca ccgccaggcg acatatacag
    96841 ttcaaataat ttgcgcactt cgcccacttc atagcccgtc tcttcaatcg cttctttacg
    96901 aatgcacact tccggttcgt cgttatccag cagcccggcg caggtttcaa tcagctgccc
    96961 gctttcattg ccattaaccc aggtagcgac acggaactga cgaatcagaa ccacgctctt
    97021 tttcttcgcg ttgtacagga ggatcgtcgc accattaccg cgatcgtaaa cttcacgttt
    97081 atggcggata acttcaccat ctttgcgcgt gagatcgtaa gtaatgttgt gcagggtgaa
    97141 atagttatcg gagagaattt tgtctttaat gagggtgatt tgttgcgtca taccgactcc
    97201 acagcgcgaa atgaacaatt atattacgct gtgaagtcgg gtttgtctgc gcaggctatc
    97261 agtgagattt gactgatttt acccccagcc agtcaataat cccctgtgcc gcatgacgtc
    97321 cttctgccat tgcggtaacc accagatccg caccacgcac ggcgtcgcca ccggcgaaga
    97381 ttttcggatt ggtggtctgg taacggtact ggctttccac atccgcgatg atgcggcccc
    97441 atttgtctac cgttacaccg tgcgactcca gccacggcat cccgtgtgga ttgaagccaa
    97501 acgccataat caccgcatcg gctggcatga caaattcact gccctcaatc ggtacaggtc
    97561 gccgacgccc ctgggcatcc ggctcgccaa gacgcgtgcg caggaaacga attccgcaaa
    97621 cgtgaccttg ctcattcagc tcaagcgcca ccggctggac gttaaattcg aagttggccc
    97681 cctcttcgcg ggcgttcttc acctctttct tcgagcctgg catgttagct tcatcacgac
    97741 gataagcgca ggtgacgtta ctcgcgccgt ggcgcagcgc agtacgcaca cagtccatcg
    97801 cggtgtcgcc gccgcccagt accacgacgt taagtccggc ggtattgata aacggctctt
    97861 ccggtagctc ttcgagcccc atcacctgtt tagtgttggc aatgatgaac ggcagcgcgt
    97921 cataaacgcc cggcgtatct tcattgggta aacccgcttt catggaacgg taagtgccta
    97981 cgccaacgaa gaccgcgtcg tattgttcca aaagcgaatc caaagagaca tctttaccca
    98041 cttcacaatt gagttcgaag tgaatcccca tcgcgctgaa gatttcccga cggcgtgcca
    98101 gcagggattt atccagtttg aaagaaggaa tgccgaaagt gagcaagcca ccgatttctg
    98161 gatggcgatc gtacaccgtc accgccacgc cattgcgggt cagaacatcc gcacaggcca
    98221 gccctgccgg acctgccccg ataatcgcca cccgcttgtc cactttggtg acatgactta
    98281 agtcaggacg ccaacctttc gccaacgcct gatctgaaat atagcgttca atgttgccga
    98341 tagttaccgc gccgtgctca tcgcgaatag tacaggcacc ttcacacaaa cggtcttgcg
    98401 gacaaacgcg tccggtaatt tccggcaggg tgttggtctg gtgagaaagc tcgacggcgg
    98461 cgtcgatgtt tccggctttc accagttcaa tccactgcgg aatatggtta tgcagcgggc
    98521 aggtccattc acaaacgctg tgctcgccga acttaaggca gcgcgaggct tcccgttgtg
    98581 cctggtcggc gcgaaatggc agataaattt catcaaaacc ggttttgcgc gcttcaatcg
    98641 ccagtttatc cggctcgcca cgcgcgggcg ttgcctgcat ttgttcgact ttactcatta
    98701 ccggcatttc ttgtgcagcg gtactggcat gccacggttg atgctcctga cgtgcggtgc
    98761 gcaagcggcg ggatttcgcc atgccggaga gtgcgacgtc agtgaccagt tgcagcgcgt
    98821 ccgccgggca attctcgaca caggcaggac cgttttcgcg ccctgcacaa aggtcgcatt
    98881 tatgcgccgt ggcttttact tttcctgccg cgacgggtgt caggacgatt tgcatcgtac
    98941 caaacggaca ggccaccacg caggatttac agccaataca cttttgctga ttgacctgaa
    99001 tgctgtcatc aacgtggctg attgcgccat tagggcagct acgggcgcag ggcgcatctt
    99061 cacaatggtg acaggtcact gcactacgtt gctgctgatg tttgataacc gtaattcggg
    99121 gatgaaaatg gtgttggctc aggacatgtt gctcatcatt gtgagccatg acacaggcga
    99181 tttcacaagc atgacaaccc agacattgct gactgttggc cataataaaa cgattcataa
    99241 cgaccttctt ttttggttgt aaaaacctta ttctttatat gagtgttgtt attacccgac
    99301 ttacagggga accggcaatg ttcatgtgcc cagaataagt aactatttcg ctataaactg
    99361 tggcagatca aataatccca tcactgacta aattgcgttt cagcgaactg gaacattaat
    99421 gattttttgt ggagaagacg cgtgtgattg ttaaacgccc cgtctcggcc agtctggccc
    99481 gggccttttt ttacattgtg ctgctgtcga ttctttccac gggtatcgct ctgctaactc
    99541 tggcgagcag tttgcgcgac gctgaggcta tcaatattgc cggatcgctg cgtatgcaga
    99601 gttaccgcct gggctacgac ttgcaaagtg gcagtccaca actcaatgca catcgccagt
    99661 tgtttcagca ggcactgcat tcaccggtat taaccaacct caacgtctgg tatgtgccag
    99721 aagcagtaaa aactcgctat gcgcatctga atgccaactg gctggagatg aataatcggc
    99781 tcagcaaggg cgatttgccg tggtatcagg ccaatattaa taattatgtt aatcagatag
    99841 acctgttcgt gctggcttta cagcactacg ctgaacgcaa aatgctgctg gtggtggcga
    99901 tttccctggc tggcggtatc ggtattttca cgctggtctt ttttactttg cgccgcatac
    99961 gccatcaggt ggttgccccg ctgaatcagc tggttaccgc cagtcagcgt attgaacacg
   100021 ggcagttcga ctcgccgccg ctggatacca gtctgccgaa tgagcttggt ctgcttgcaa
   100081 aaacctttaa ccaaatgtcg agcgagctgc ataaattgta cctttcgctg gaggcgtcag
   100141 tagaagaaaa gacccgcgat ctccacgagg ccaagcgtcg tctggaggtg ttgtatcagt
   100201 gcagtgttcc caggcgctga acaccagcca gattgatgtg cattgtttcc gccatatttt
   100261 gcagattgtt cgcgacaatg aagcagctga atatctggag ctgaacgttg gggataactg
   100321 gcggattagc gaagggaagc caaaccctga gcagccaatg cagattttac cggtgacaat
   100381 gcaagagacg gtttacggcg aactgcactg gcaaaatagt cacgtttcat catcagaacc
   100441 gctgcttaac agtgtttcat cgatgctggg acgcggtttg tactttaatc aggcgcagaa
   100501 gcattttcag cagttattgt tgatggaaga acgtgcgacc atcgcccgcg aattgcacga
   100561 ctcgctggct caggtgcttt cttacttacg tatccagttg acgttactga agcgttcgat
   100621 accggaagat aacgccaccg cacaaagtat catggccgat ttttcccagg cgttgaatga
   100681 tgcttatcgg cagttacgcg agctgttgac tactttccgc ctgacgctgc agcaggcgga
   100741 tctcccctcc gcgttgaggg aaatgctgga tacgttacaa aatcaaacca gcgctaaact
   100801 gaccctcgac tgccgtctgc caaccctggc actggatgcg caaatgcagg tgcatttgtt
   100861 gcaaattatt cgcgaagcgg tgctgaatgc gatgaagcac gccaacgcca gcgaaatcgc
   100921 catcagttgc gtcaccgcgc cggacggcaa tcacacggtt tatatccgtg ataacgggat
   100981 tggtatcggt gaaccgaaag aacccgaagg tcattatgga ctgaatatca tgcgcgaacg
   101041 cgcggaacgg ctgggcggga cgctgacttt ttcacaacct tccggcggcg gcacgttagt
   101101 gagtattagc tttcgctccg cagagggtga ggggaagggg aaggggaaca cgccggctga
   101161 ggtgtaaata attaccctca caccggccct ctccctttaa aggcgagagg gaaaaacgtg
   101221 cccatattga cctgtaagcg agtgtgtcag caatctcatg ataatttaca ttaactcctt
   101281 tttttctcca cgattggctc gtaccttgcc gctacagtga agcaagtcaa gcctacaaca
   101341 atacgcagaa acacgaggtc ctcttttaat ggcgaatttc tttattgatc gccccatttt
   101401 tgcctgggtg ctggcaatcc tgttgtgtct gacaggtacc ctggcgattt tttcattgcc
   101461 cgttgaacaa taccccgatc tcgcgccacc gaatgtgcga gtgaccgcta actatcccgg
   101521 cgcatcggcc cagacgctgg aaaacaccgt gacccaggtt atcgagcaaa atatgaccgg
   101581 cctcgataat ctgatgtata tgtcatctca gagcagtggc accggtcagg catctgtcac
   101641 tttaagtttt aaagcaggca ccgatccgga cgaagccgtg cagcaagtac aaaaccagct
   101701 gcaatcagcc atgcgaaagt taccgcaggc ggtgcaaaat cagggcgtga cggtgcgtaa
   101761 aaccggcgat accaacattc tgaccattgc cttcgtctct accgatggct cgatggataa
   101821 acaggatatt gctgattatg ttgccagtaa tattcaggac ccgttaagcc gcgtgaatgg
   101881 cgtcggggat atcgatgcct atggttcgca atattccatg cgtatctggc tggacccggc
   101941 gaaactcaac agtttccaga tgacggctaa agatgtcact gatgccattg agtcacagaa
   102001 cgcgcagatt gcggttgggc aacttggtgg tacaccttcc gtcgataagc aggcgctcaa
   102061 cgccgccatt aacgcccagt cactgctgca aacaccagaa cagttccgcg atatcacctt
   102121 gcgggtgaat caggacggct cagaggtaag gctgggcgat gtcgccaccg tcgaaatggg
   102181 ggcggagaaa tacgattatc ttagccgctt caatggtaag ccagcctccg ggttgggggt
   102241 aaaactggcc tccggcgcaa acgaaatggc gacagcggag ctggtgctca atcgtctcga
   102301 cgagctggcg cagtatttcc cgcacggact ggaatacaag gtggcgtatg aaaccacctc
   102361 gtttgttaaa gcctccattg aagacgtggt gaaaacgctg ctggaagcta tcgctctggt
   102421 tttcctcgtt atgtatctgt tcctgcaaaa cttccgcgcc acgctgatac ccactatcgc
   102481 cgtgccggtg gtgttgatgg gaaccttctc cgtactttac gccttcggtt acagcgtcaa
   102541 caccttaacc atgttcgcga tggtgctggc gatcggtctg ctggtggatg acgccatcgt
   102601 ggtggtggaa aacgtcgaac gtattatgag tgaggaagga ctcactcctc gcgaagccac
   102661 acgtaaatcg atggggcaga tccagggggc actggtcggg attgcgatgg tgctttcggc
   102721 ggtatttgta ccaatggcct tcttcggcgg caccaccggt gccatctatc gccagttctc
   102781 tattaccatt gttgcggcga tggtgctgtc agtactggta gcgatgatcc tcactccggc
   102841 tctgtgtgcc acgctactta agccactgaa aaaagctgag catcacgggc aaaaaggttt
   102901 ttttgcctgg tttaaccaga tgtttaaccg caacgccgaa cgctacgaaa aaggggtggc
   102961 gaaaattctc caccgtagcc tgcgctggat tgtgatttat gtcctgctgc ttggcggcat
   103021 ggtgttcctg ttcctgcgtt tgccgacgtc gttcttaccg ctggaagacc ggggcatgtt
   103081 tactacctcg gtacaattgc ccagcggttc aacccaacaa cagaccctga aagtcgttga
   103141 gcaaatcgag aaatattact tcatccatga aaaagacaac atcatgtcgg tgtttgccac
   103201 cgttggttct ggccctgggg gtaacgggca aaacgtggcg agaatgttta tccgcctgaa
   103261 agactggagc gaacgcgaca gtaagaccgg cacctcgttt gccattatcg agcgtgcaac
   103321 gaaggcgttt aaccaaatta aagaagctcg cgttatcgcc agcagcccac cagcaattag
   103381 cggtcttggt agttctgcag attttgatat ggagttgcag gaccacgctg gagcgggtca
   103441 cgatgcgctg atggcagcac gtaatcagtt gctggcgctg gcggcggaaa acccggagct
   103501 aacccgtgtg cgccataacg gcctcgatga cagtccgcag ttgcagattg atatcgacca
   103561 gcgtaaagct caggcgctgg gcgttgatat cgacgatatt aacgacacac tgcaaaccgc
   103621 ctggggttcg agctatgtga atgactttat ggatcgcggt cgcgtgaaga aagtctatgt
   103681 gcaggcagct gcgccgtatc gcatgctgcc agatgacatc aatctctggt atgtccgaaa
   103741 taaagatggc ggcatggtgc ccttctctgc tttcgcgacc tcacgctggg aaacaggctc
   103801 gccgcgtctg gaacgctata acagttattc tgcggttgag attgttgggg aagccgcacc
   103861 gggggtcagt accggtacgg cgatggatat tatggaatcg ttagtgaagc agctgccaaa
   103921 cggctttggt ctggagtgga cggcgatgtc gtatcaggag cggctttccg gcacgcaggc
   103981 tccggcgctg tacgccattt ccttgctggt ggtattcctg tgtctggcgg cattgtatga
   104041 aagctggtcg gtaccgttct cggtaatgct ggtcgtgccg ctgggggtaa tcggcgcgct
   104101 gctggcaacc tggatgcgcg ggctggaaaa cgacgtttac ttccaggtgg gcctgttaac
   104161 ggtcattggt ttatcggcga aaaacgccat cctgatcgtc gagtttgcta acgagatgaa
   104221 ccaaaaaggc cacgacctgt ttgaagcgac gctccacgcc tgccgtcagc gtttacgccc
   104281 gattctgatg acctcgctgg catttatctt cggcgtattg ccaatggcaa ccagcacggg
   104341 tgccggttcc ggtggtcagc atgcggtggg tactggcgta atgggcggga tgatttcagc
   104401 tactattctg gctatttact tcgtgccgct gttctttgtg ctggtgcgcc gccgcttccc
   104461 gctgaagccg cgcccggaat aagcaataaa aaaggcgaca tgccaatgtg tcgccttttt
   104521 caactttccg attaagaacc tgctcagcgg gttcttgctg tttatacttt gtctcaggaa
   104581 ttacttacga agcataactt cgataaagtc tttccagttc cccagttcac gttcaatcat
   104641 aacaacctct cttataatta tgggtattct acggaaaaaa tataccgtgg tgaagctaat
   104701 ttactcgatt gctgcgatga ctacctccgg gggacaaacc ttatgtaaat actatggtcc
   104761 tgcggtgata atttgtatgt gatacacagc aacatttcga gatattcata cggcatctaa
   104821 tacttattta attctggtta aaatacagac agataacaag atgaatattc ttaatgttta
   104881 cgttaaaaat gtttaatatt atttaatagt tgttaatttg aatacttcga taatgttata
   104941 ttttctgata atcatttgca ggcaaaatgt tttcaccctt aaatgagtat ttattctcat
   105001 aaatcgaaaa aggattcatt atggttacac tttacggcat caaaaattgt gacaccatta
   105061 aaaaggctcg ccgttggctg gaagccaata acatcgacta tcgttttcat gattaccgcg
   105121 tcgatgggct ggacagcgaa ttattgaacg gttttatcaa cgaattaggc tgggaagcgt
   105181 tactcaacac ccgtggtaca acctggcgta aactggacga aactacccgc aataaaatca
   105241 ccgatgcggc ctctgcggcg gcattaatga ctgaaatgcc tgcaattatc aaacgtccat
   105301 tgctctacgc gcctggtaag cctatgctgc tgggtttcag tgattccagt tatcagcaat
   105361 ttttccatga ggtgtagtct atgtcgtgcc cggttattga gctgacacaa cagcttattc
   105421 gccgcccttc cctgagtcct gatgacgcag gatgtcaggc tttgttgatt gaacgtttgc
   105481 aggcgatcgg ttttaccgtt gaacgcatgg attttgccga tacgcagaat ttttgggcat
   105541 ggcgtgggca aggtgaaacg ttagcctttg ccgggcatac cgacgtggtg ccgcctggcg
   105601 acgccgatcg ttggatcaat ccgccgtttg aacccaccat tcgtgacggc atgttattcg
   105661 ggcgcggtgc ggcagatatg aaaggctcgc tggcggcgat ggtggtagca gcagaacgtt
   105721 ttgtcgcaca acatcccaac catacggggc gactggcatt tctgatcact tctgatgaag
   105781 aagccagtgc ccacaacggt acggtaaaag tcgtcgaggc gttaatggca cgtaatgagc
   105841 gtctcgatta ctgcctggtc ggcgaaccgt cgagtatcga agtggtaggt gatgtggtga
   105901 aaaatggtcg tcgcggatcg ttaacctgca acctcaccat tcatggcgtt caggggcatg
   105961 ttgcctaccc acatctggct gacaatccgg tacatcgcgc agcacctttc cttaatgaat
   106021 tagtggctat tgagtgggat cagggcaatg aattcttccc ggcgaccagt atgcagattg
   106081 ccaatattca ggcgggaacg ggcagtaaca atgttattcc gggtgaactg tttgtgcagt
   106141 ttaacttccg cttcagcacc gaactgactg atgagatgat caaagcgcag gtacttgccc
   106201 tgcttgaaaa acatcaactg cgctatacgg tggattggtg gctttccggg cagccatttt
   106261 tgaccgcgcg cggtaaactg gtggatgcgg tcgttaacgc ggttgagcac tataatgaaa
   106321 ttaaaccgca gctactgacc acaggcggaa cgtccgacgg gcgctttatt gctcgcatgg
   106381 gggcgcaggt ggtggaactc gggccggtca atgccactat tcataaaatt aatgaatgtg
   106441 tgaacgctgc cgacctgcag ctacttgccc gtatgtatca acgtatcata gaacagctcg
   106501 tcgcgtgatg agtggttctg caagaggaaa taagcatgga ctggctggct aaatattggt
   106561 ggattctggt gattgtcttt ttggtcggcg tcctgctgaa cgtgattaaa gatctcaagc
   106621 gcgtcgacca taagaaattt ctcgccaaca agccggagct tcccccgcat cgtgatttca
   106681 acgataagtg ggacgatgac gacgactggc cgaaaaagga tcaaccgaag aagtaagctg
   106741 agtgctcaac cgtaggccgg ataaggcgtt aacgccgcat ccggcaatgg tgaacgatgc
   106801 ctgatgcgac gctcacgcgt cttatcaggc caggtaatct gtcttttaca tcatttcaat
   106861 cacatcatca tcgccaggct taccgccact tagcgcttgg tcgaaataat gtttcggaat
   106921 ggtataacgc aagtgatcca gggcaaattg catactgcga ttgtcgatcg catggcctaa
   106981 atcctccacg atatccagcg tcacgtcacc accggcactg attaacgctt cctgcgccgc
   107041 aactgcatgc gccagatcga taaccggatc ttcaccacca tgaatcaagt gaattgtcgt
   107101 ggctgtagac gcggtctccg gcaggctcgc atagcgtccg ttaaaggcaa tgacgcgcga
   107161 agcaaggccc ggttcggctt taatgctctc cagcgccata atcgcgcctt gtgagaaacc
   107221 aattagcgcc gtggcgttag cccctactcc gctctgtttc tgccagtagc gtaccgtttc
   107281 aataaacgtc ggcatgatgg catccacacg cgcctggcga ttatcttccg taataccctg
   107341 cactgaaaac cactgacgcc ccgccggatt accgctcggc tccgcgccac cgacactcac
   107401 caccagcgca tcaggaaaca gcggtgcaaa ccagctgcct atttccccca ttgccaccgg
   107461 gttatccccg acaccgtgga aaagcagtaa caactgttgt gcaggtttat ccgggctttg
   107521 aacaacaaaa tggtcatgtt tcatggcgaa ctccttaact gatgtcatta attttacgcc
   107581 gctgactcac aatgaccatg ggagaaaatt gaaggagtca gtgaaaaaat tgccattgcg
   107641 ttatgcgatc gcgcagacgc tcagtgcgaa cctcatttag tgcgaacagc gcctgcgcgg
   107701 cctcttcccg ctggcgaacc agtaacatct tgcggcctga aagtttaagt gtggtacata
   107761 actgcgcgtc actggcgttt ttctgtaaac acccacgcag cgccggtaat gccagttcac
   107821 tggtttgtag cagacgcaat aagcaaccta acgatgttaa tagcggacga tgagcgaaag
   107881 caaaaccggc cagttcaagc cagtcgtcgt cagaaaggat ggcatcgttt agtggatcaa
   107941 caggaagcat ttcgccattc cactgcgcga gagcttgcgc atcgcgacgt aaacggtaat
   108001 gctcacgttc agccagctgt ttacccgcat cactcatcgg taacagcgcc atcgccgtat
   108061 agcaaccgct gctggcttcc cgatgattac ccatccgcac cagcacaaaa ccgcagcgtt
   108121 gccagaaacg ccataactcc ccagtgtaac caaaactcac cgaaagatag tcgaggtcat
   108181 gcgtatattg caaagctcca gcaataagtt gccgccctgt gccttcccgc tgacgagccg
   108241 gatgaactgc tatccggcta acccgccttc cgcgcaatgt cgccgccagt ggattgctgc
   108301 cgtgcgccgc cagcgactgg gccaccagat taccccgcgg gcgacgaaaa cctgcccata
   108361 ccgcctgact gagttcttga gataatccgc cctcatccac cagccacaac gccccggcaa
   108421 tctcgttttc gccagccgcc tgtaaaaaat gttgccctag tgcatccatc atccggcgta
   108481 aatccagcgg cgaggtccgg tagtgcgcac cagacaataa ctgataaacc tttaacggcg
   108541 tttctggctc gcttcgccat aacgtttgtt caaatgcgga aatgacgata ttgccttgtg
   108601 gtgtatgggt gaagttttca tcgttaaaaa ccagtgcctc actaaccatt ttttccagcg
   108661 ggcatccctg tgcccagcgg atcggctgtt gcagttcaaa acggtgtaaa tgcggaaagc
   108721 gagcgcaaaa tttcagcaaa aaaccacgtc cggtaccttc gtagccctgc accgtagtgg
   108781 ttaacaacgt tcgaggaaaa cgcgatacca gttgatgcaa caacggcgca ggtatggctg
   108841 cggcttcgtc gaccaccagc cagtcggctt gctcatcgct ggctaacaag gcatccggtg
   108901 caataaagcg aaacttctcg cccgcaaatt gtgccagtac atccgttgcc gcttttgcgg
   108961 gcgcggtgac aatcgcactg cccgcaatac gagaaatgag ttgccctgcc aacgccgact
   109021 taccgcgccc acgcgcagcc gttaccgctg ccacgcccgg cggcatggtc agtagctgct
   109081 gtaagagttg ctgttgttct ggttgtggtg cgccggtcgc ggggtgccag tcagtacggg
   109141 gagtaaaatg cgccaacgag aacggctggt tttgccgcca gaggatagcg tcgttatccg
   109201 ccgtaagtac gcgtttgaga tgctggacaa aatgcggcgt cgcaataggg tcagggcaat
   109261 cactccagcg cagcgagtcg gcatcaggtt ggttttccca ctcttcccat acagggagta
   109321 acaaaaccag ccagcttccc gctttcaacg ttccgctaag tgctgcaaag gcagcggcat
   109381 caaagccgtg gcgggcgtcg aataccgcat gccggaactc gcgcccaagt aaagtttgta
   109441 gcgccgaggg tgaacagtgg ttttcagcat ctagctgcgg cgaaatccac agccagtcgc
   109501 caggtaaggc atcacgcaac ttaagagcat gatcaaaaca ccaaccctct tctccgctca
   109561 acaccagcaa gcggcagatc ccttcacgtt tcatttgcgc tgttaatgtg taaagcgcag
   109621 tcagttcagc catccctgcc ccggaaatta aatgctttta ccaaaagtat tgcattgtgc
   109681 cggatcgccg ctgtcgaaac cacgtttaaa ccagctgcag cgttgctgag aagtgccatg
   109741 tgtgaaactg tctggtacta ctcgcccctg actttgctgt tgtaaacggt catcgccgat
   109801 ggcctgcgcc gcattcagcg cctcttccag atcgccagtt tccagaacgc cttgctgctg
   109861 catactatgc ccccagacac cggcaaaaca gtcggcctgg agttccatac gcacagataa
   109921 gcggtttact tccgcctgcg tcgcgttttg ttgcagttga cgaactttcg gctcgatgcc
   109981 taacagtttc tgcacatgat gaccgacttc atgggcgata acgtacccct gggcaaaatc
   110041 gccatccgcg ccaagtttgt ctttcatgtc atcatagaag gagagatcga tataaactgt
   110101 gccatccgcc gggcaataga acggccccat aatggactgg cccgccccgc agccggtgcg
   110161 cgtcattcca cggtacatga ccagcttcgg ttgctgatag gtcttaccca tcttttcgaa
   110221 ctgttgtccc caggtgtctt ccgtggttgc cagaatcacc gaggtgaatt ttgcggcttc
   110281 gtcttcatta gggctaattg accgcgttga ttgttgttga gaaaccggct gcccggtcat
   110341 caacccggtt aaatcaacgc catagtagcc tgcaaccagc acgactatca gtaaaatcag
   110401 cccgccttta ccgctcggca gacggaaacc gggaccgccc attgacggac cgccagagct
   110461 gttgcgcctg tcttcaacat tgtcactttc acgtcgccct tgccaacgca tagatacctc
   110521 aacaatatat tcattataga aatgatcgta ggtggttcag aggaagatta ccacaggaaa
   110581 gcgggatgaa atggcggagg aatgagcgtt caatgcatat attccgagag cggttgtctt
   110641 gccgtgccag ctgcacggca agatgatgaa cagaaaaatc agtccagctg tacacccagg
   110701 cggcgggcga cggcttcata ggcttcgatc aggccaccga ggctctggcg gaaacggtct
   110761 ttgtccattt tctccagtgt ttctttgtcc cacaggcggc tgccgtccgg ggagaactca
   110821 tcgcccagta ccacttcacc tttgtacaga ccaaactcca gcttgaagtc gaccagaatc
   110881 agaccagcat catcgaacag ttttttcagc acgtcgttcg ctttgtaggt cagctctttc
   110941 atacgtgcca ggttctcttt gctcacccag ccaaaggttt cgcagtaaga ttcgttgacc
   111001 atcggatcgt gcatggcatc gtttttcagg aacagatcga acagcggcgg gttcagctca
   111061 ataccttctt cgattccaag acgtttcacc agagagccag cagcacggtt acgcacgaca
   111121 cactcaaccg gcaccatatc cagctttttc accagacatt cggtatcgga gagcagacgc
   111181 tccatttgag tcgggatacc cgcttcagcc agtttgctca taatgaagta gttgaacttg
   111241 ttgttcacca tacctttgcg atcaaactgc tcaatgcgcg cgccatcccc tgctgacgta
   111301 tcattgcgga attcgagcac caacaggtcc gggttttccg tgctgtatac ggttttcgct
   111361 ttaccacgat acaactcagc ttgcttttgc atctttatca ctcctgggtg tgaattaacg
   111421 ttttaaaatc ttttgctgtc tggtgtgccg gatgtttttt cggatgcggc gtgaacgcct
   111481 tatccgaact acgtccgaca aacaaatttc gtgcgaatta ccgctaaaat cgcgttttcc
   111541 tgccgacgca cacgtttgcg tatcatatca gaaaaaaggg ccggatgatt ccagccctgt
   111601 atttttactt gctaaacgca gcctggaaga cagctaccag cgcgtcgttc tgactctgag
   111661 tcagagtatg acctttcgga tcgatgaact gtaggctgct gcggttatct aaatcgccaa
   111721 cctgcagttt atagtcaccg gatgccaggc ctggatcgct cgcgcccagt tcctgccagt
   111781 cgctgtcaga cagcggctta taagttacgg ccatgttgcc ctgcgaacgg gtgctgtcgg
   111841 tcactttcat gcccactttt tccagcgacg ctggcagacg ttgccaaacc acattgaacg
   111901 gcccgcgtac gaccagcatt ggtaaaccgg tgtcatcagc tgcactttgt acgtccatag
   111961 tggtggaggc acgattttgc gcagcgttcg cggcgtcagt ggcagattta tccagaccgg
   112021 cggaaataac gttcatcatc tccgtgctgt aacgctgcat ggaagccgcg tctgcaaccg
   112081 gtttgcccgc ctgttccagg ttcagcagtt taaccgtaac cgcctgctga taaccctgcg
   112141 gcttaacaga gatttgataa cgaccacgat actgctcgtc ttcgtccaga cggttccatt
   112201 gtacccaatc ggtggtcagt gtctgaccag catcatcacg ttgggtgatg gtgtagtttt
   112261 tcgcctgcag cacgctaacc acctgcggcc acagagtatt gccacgacca ttttccacca
   112321 gcaatgaagc ggtatcgccc gtgaactggg tacgcgcgcc agaaaccagt gccagcggct
   112381 gggctggtgg acgaatgtcc agcgccttac cgacagcacc actaccgttg gtcaccggga
   112441 ttgcataatc accggaggtc accggcaaaa tcattccagc cggggcatga agctccgcaa
   112501 gcggtgccgc ttccaggtag gcttcatcac cactgacctg acgcttatag cgtgagtcag
   112561 aactacaggc agcgagtaat aaaacaagcg aaacacccgc aacctttgcc aggcgcgact
   112621 tttgaacaga gtaagccatc aaatctccct aaactttaca gcaaaccggc atgcttaagc
   112681 gccgctctga ccgtctcacg accactgtcg gtgattggtg tcattggcag gcgcagcgta
   112741 tcggtcgcca caagacccag ttccttacat gcccatttca ccgggattgg attgggttcg
   112801 acaaatagtt tgttgtgtaa tggcatcaga cgctgattaa taacgcgtgc ctcggcaaaa
   112861 tgcccttctg ctgccagttt gcacatctgg gccatatcac gcgctgcgac gttagccgta
   112921 acggaaataa ccccatgacc gcccaattgc atgaagtcca gcgcgctcgc atcatcgccg
   112981 ctcagcagaa caaaatcatc tgaaaccagc tctttgatct ggtttacacg cgttaagttc
   113041 cctgttgcct ctttgattcc gataatattt tttactttcg ccagacggcc caccgtttcc
   113101 gggagcagat cgcagccagt acgggacggc acattataca gaatttgcgg caggtcagta
   113161 tgctcagcga tggctttgaa atgctgatac aaaccttctt gcgacggacg attgtagtaa
   113221 ggggttaccg tcaggcagcc gacgatacca ctgtcattga agcgctgcgt caggctaatg
   113281 gcttccgcag tagcgttagc gccggtcccg gcaattaccg gaatgcgccc atcagccaga
   113341 tccagcgtca tcatcaccac atcagcatgt tcgtcatgat ttaaggtagc ggactcgcca
   113401 gtggtgccaa cagaaacgat cgccgaagta ccgctggcga catgataatc aatcagtttt
   113461 ttcaagctag cccgacagac attacctttt tcatccatcg gagtaacaat cgcgacaata
   113521 cttcccgtga acatgggcca tcctctgtgc aaacaagtgc ctcaatggta cgtttggtat
   113581 ggcattaaaa gcaagcagac agaaccgttc tgattgttgt atgcatgatt tttttatgct
   113641 ttccttaaga acaactcacc ccttaaagga ataaccagtt tgacactgtc atcgcaacat
   113701 tatctggtga tcactgcgtt gggtgccgat cgccctggaa ttgtgaacac catcacccgt
   113761 catgtcagta gttgcggctg taatattgaa gacagtcgcc tggcgatgct gggagaagag
   113821 ttcacgttta tcatgctgct ttccggttca tggaatgcca tcactctgat tgaatcaacg
   113881 ttaccgttga aaggtgccga actggatctt ttaatcgtga tgaagcgcac gacggcgcgt
   113941 ccgcgtccgc caatgccagc atctgtctgg gttcaggtcg atgttgcaga ctccccgcat
   114001 ttaattgaac gcttcacagc acttttcgac gcgcatcata tgaacattgc ggagctggtg
   114061 tcgcgcacac aacctgctga aaatgaacgg gctgcgcagt tgcatattca gataaccgcc
   114121 cacagccccg catctgcgga cgcagcaaat attgagcaag cgttcaaagc cctatgtaca
   114181 gaactcaatg cacaaggcag tattaacgtc gtcaattatt cccaacatga tgaacaggat
   114241 ggagttaagt aatgaatcca ctgaaagccg gtgatatcgc accgaaattt agcttgccgg
   114301 atcaagacgg agaacaagtt aatttgaccg acttccaggg acagcgtgtt ctggtttatt
   114361 tctacccgaa agccatgacc cccggctgta ccgtacaggc ctgcggctta cgcgataaca
   114421 tggatgagtt gaaaaaagcg ggcgttgatg tgctgggtat cagcaccgat aaacccgaaa
   114481 aactctcccg ttttgcggaa aaagagctgc ttaactttac gctcctgtct gatgaggacc
   114541 accaggtgtg cgaacaattc ggcgtctggg gtgaaaagtc cttcatgggc aaaacctacg
   114601 atggtattca tcgcatcagc ttcctgattg acgctgatgg caaaatcgaa catgtctttg
   114661 acgatttcaa aaccagcaat caccacgacg ttgtgctgaa ctggctgaaa gaacacgcct
   114721 gattactttg ctccattccg tgctggctgc gcttgcggcc agcatacctc acttctcgtg
   114781 atcaagatca cattctcgct ttcccctgcg acacgggtgt cgaatccatt ttttgctgaa
   114841 cgttaatgac catcattttt gtaccgttca gaatccagtt aatacataac ttattgaata
   114901 gattgagtta atcagaatgg caccctttat gcaatatgaa atgcaatgtt tcatatcatt
   114961 ttcaaggagc cgacatgaac cgctttgtgg tggccgaacc actgtggtgt acaggatgta
   115021 atacctgtct cgctgcctgt tcggacgtgc ataaaacgca aggtttacag caacacccgc
   115081 gcctggccct ggcgaagacg tcaacaatca ctgcccctgt cgtgtgtcat cactgtgagg
   115141 aagccccttg cctgcaggtc tgcccggtca atgccatctc tcagagggat gatgcgatcc
   115201 aactcaacga aagcctctgt attggctgca agctttgcgc cgtggtctgc ccatttggcg
   115261 caatcagcgc ttcaggaagc cgtccggtga atgcccatgc gcaatatgtt tttcaggctg
   115321 aaggctcact caaagacggc gaagaaaacg tgccgcccca acatgcgttg ctgcgctggg
   115381 aaccgggtgt ccagaccgtc gcggtgaaat gcgacctgtg tgatttcttg ccagaaggtc
   115441 cggcctgcgt tcgcgcttgc ccgaatcagg cgttacggct gatcaccgac gatagcctgc
   115501 aacgtcagat gaaagaaaaa cagcgccttg ccgcaagctg gtttgccaat ggcggggagg
   115561 atcccctttc cctcactcag gagcaacact aatggatgcc ctgcaattat taacctggtc
   115621 gctgatcctc tatctgtttg ccagtctggc ttcgctgttt ttactcggtc tggacagact
   115681 ggctattaag ctttccggca tcacatcgct ggtgggcggc gtgattggca tcatcagcgg
   115741 aattacgcaa ttacatgctg gtgtaacttt agtcgcccat tttgccaccc cttttgactt
   115801 tgccgattta accctgcgaa tggatagcct ctcggcattt atggtgctgg ttatctcctt
   115861 gctggtggtg gtttgttcgc tctattcatt gacttatatg cgcgaatacg agggcaaagg
   115921 cgcggcggcg atgggcttct ttatgaatct tttcatcgca tcgatggttg ccctgctggt
   115981 gatggacaac gctttttggt tcatcgtgct gtttgaaatg atgtcgctgt cttcctggtt
   116041 tctggtcatt gccaggcagg ataaaacgtc gatcaacgct ggcatgctct acttttttat
   116101 cgcccacgcc ggatcggtgc tgattatgat cgccttcttg ctgatggggc gcgaaagcgg
   116161 cagcctcgat tttgccagtt tcgcacgctt tcactttctc cggggctggc gtcggcggtg
   116221 tttctgctgg cctttttcgg ttttggcgcg aaagccggga tgatgccgtt gcacagctgg
   116281 ttgccgcgcg ctcaccctgc cgcaccatcg cacgcttcgg cgttgatgtc tggcgtaatg
   116341 gtcaaaatcg gtattttcgg catcctgaaa gtagcgatgg atctgctggc gcaaacgggt
   116401 ttgcctctgt ggtggggcat tctggtgatg gcgatcggcg caatctccgc gctcctgggc
   116461 gtgctatatg cgctggcgga acaggatatc aaacggctgc tggcctggag taccgtcgaa
   116521 aacgtcggca ttattttgct ggcagtcggt gtggcgatgg acggtctgtc actgcacgac
   116581 ccgctgctca ccgtggttgg actgctcggc gcactgtttc atctgctcaa ccatgcgctg
   116641 ttcaaagggc tgctatttct cggcgcggac gcgattattt cgcgtttgca tacccacgac
   116701 atggaaaaaa tgggggcact ggcgaaacgg atgccgtgga cagccgcagc atgcctgatt
   116761 ggttgcctcg cgatatcagc cattcctccg ctgaatggtt ttatcagcga atggtacacc
   116821 tggcagtcgc tgttctcact aagtcgtgtg gaagccgtag cgctacaact tgcgggtcct
   116881 attgctatgg taatgctggc agtcactggt gggctggcag taatgtgctt cgtcaaaatg
   116941 tacggtatta ctttctgtgg tgcgccgcgc agtacacacg ctgaagaggc acaggaagtg
   117001 ccaaatacga tgatcgtcgc catgctactg ctcgcggcac tctgcgtatt aattgcgctt
   117061 agtgccagtt ggctggcacc gaagataatg catattgccc atgcgtttac caatacccct
   117121 cccgtcactg tcgccagcgg aatagcactt gtacccggca cgtttcatac acgggtcact
   117181 ccttcattac tgttgctgtt actactggcg atgcctttgc tgccaggcct ttactggctg
   117241 tggtgtcgtt cgcgccgcgc agcgtttcgt cgcacaggag atgcctgggc atgcggctac
   117301 ggctgggaaa acgcgatggc tccgtcaggc aatggcgtga tgcagccgct gcgtgtggtc
   117361 ttctctgcgt tatttcgtct acgacaacag ctcgacccta cgctgaggct aaataaaggt
   117421 cttgcgcacg tcaccgccag ggctcagagc acagaaccct tctgggatga gcgggtgatc
   117481 cgccccatcg tgagcgccac ccaacggctg gtcaaagaaa tacagcatct gcaaagcggc
   117541 gactttcgtc tctattgcct gtatgtggtc gccgcactgg ttgtgctgct aatcgctatt
   117601 gccgtctaag gaaatcacca tgagacaaac tctttgcgac ggatatctgg tcatttttgc
   117661 gttagcacag gccgtgattc tgctgatgct aaccccactt tttacgggta tttcccggca
   117721 gatacgcgcg cgtatgcact cccgccgcgg gccggggatc tggcaggatt atcgcgatat
   117781 ccacaaactg tttaaacgcc aggaagttgc gccgacatct tcaggtctga tgttccgcct
   117841 gatgccgtgg gtattaatca gcagcatgct ggtgctggcg atggccttac cactatttat
   117901 taccgtttcc ccttttgcgg gcggcggcga tctgatcacc cttatctatc ttcttgccct
   117961 gtttcgtttt ttctttgctc tttccgggct ggataccgga agtccgtttg cgggagtcgg
   118021 tgccagtcgc gagttgacgc tcggcattct ggtcgaacca atgcttattc tctcactgct
   118081 ggtattggcg ctgatagcag gttccacgca tatcgagatg atcagcaata cgctggcgat
   118141 gggctggaac tcgccgctaa ccaccgtact ggcgttactg gcctgtggtt ttgcctgctt
   118201 cattgagatg ggaaaaattc cctttgatgt tgctgaagca gaacaggaat tacaggaagg
   118261 cccgctgacc gaatattccg gtgccgggct ggcgctagcg aaatgggggc aggggctgaa
   118321 acaggtcgtg atggcagcac tgtttgtggc cctgtttctg ccctttgggc gcgcgcaaga
   118381 actttctctc gcctgcctgc tgacttcact tgtcgttacg ctgctcaagg ttttgctgat
   118441 ttttgtactg gcctcaatcg cagaaaacac gctggcacgc gggcgttttt tactcattca
   118501 ccatgtgacc tggcttggct tggcttcagc cttgctgcgc ttgcatgggt cttctggtta
   118561 accggtctgt aaggagcact gacggaatat ggaaaatctt gctctgacga cgttaatgct
   118621 gccttttatc ggcgcactgg tcgtttcgtt ttcgccacaa cgtcgggccg ccgaatgggg
   118681 ggttttgttc gccgcgctga ccacgctgtg catgttgtcg ctgatctccg cgttttatca
   118741 ggccgataaa gttgccgtca cgttgacgtt ggtcaacgtg ggggatgtgg cattgtttgg
   118801 cctggtcatt gatcgcgtga gtacgctgat tctgtttgtg gtggtgtttc tcggtttgct
   118861 ggtcacgatc tactccacgg gttatctgac ggataaaaat cgcgaacacc cgcataacgg
   118921 cacgaatcgt tattacgcat ttttgctggt gtttatcggc gcgatggcgg gactggtact
   118981 ctcctcgacg ctgctcggtc agttgttgtt ttttgaaatt acgggcggct gctcctgggc
   119041 gttgatcagt tattaccaga gcgataaagc gcagcgttca gcactaaaag cgttgcttat
   119101 cacccatatc ggttcgttgg ggttgtatct tgccgccgcc acgctgtttt tgcagaccgg
   119161 aacgtttgtg cttagcgcga tgagcgagtt acatggcgac gcacgttatc tggtttatgg
   119221 cggcatcctg tttgccgcgt gggggaaatc ggcccagcta ccgatgcaag cgtggctacc
   119281 ggacgcaatg gaagcgccaa caccgatcag cgactatctc cacgccgcat cgatggtgaa
   119341 agtgggcgtt tacatttttg cccgcgccat tatcgacggc ggcaatatcc cgcatgtgat
   119401 tggcggcgtt ggcatggtca tggcactggt caccattctt tatggctttc tgatgtattt
   119461 gccacagcag gatatgaagc ggttgctggc ctggtcgacc atcactcaac ttggctggat
   119521 gttcttcggc ttgtcgctct ccatcttcgg ctcgcggctg gcgctggagg gtagcatcgc
   119581 ctacatcgtc aaccacgcgt tcgctaaaag cctgtttttc cttgtagcag gtgcgctgag
   119641 ttacagctgc ggcacgcgct tgttgccgcg tctgcgtggc gtattgcaca ccctgccgtt
   119701 gccaggcgtg ggtttctgcg tggcagcgct ggcaattacc ggtgtgccgc cgttcaacgg
   119761 cttcttcagt aaattcccgc tgtttgccgc cggttttgcg ttgtcagtgg agtactggat
   119821 cctgctgccc gccatgattc tgctgatgat tgaatcggtc gccagtttcg cctggtttat
   119881 tcgctggttt ggtcgcgtcg tgccgggcaa accgagcgag gccgtcgccg atgccgcacc
   119941 gctgccaggg tcaatgcgcc tggtgttgat tgtactgatt gtgatgtcgc tgatttccag
   120001 cgtaatcgcc gcgacctggt tgcagtaagg agatgatgaa tgaccggttc tatgatcgta
   120061 aataatctgg cgggactgat gatgctgaca tcgctgtttg tgattagcgt caaaagctat
   120121 cgcctgtcat gcggatttta cgcctgccag tcgctggtgc tggtgtctat tttcgccact
   120181 ctctcgtgcc tgttcgccgc agagcaactg ctgatctggt ccgccagcgc ctttatcacc
   120241 aaagtgctgc tggtaccgtt aatcatgact tacgctgcac gaaatattcc ccagaacatc
   120301 ccggaaaaag cgttattcgg tccggcaatg atggcactgc tcgcggcgtt aattgtcctg
   120361 ctttgcgcat ttgtcgttca gcccgtgaag ctaccgatgg ctaccgggct gaaaccggcg
   120421 ctggcggtag cgttaggtca ttttctgctt ggcctgctgt gcattgtcag ccagcgcaat
   120481 atcctgcggc aaatttttgg ttactgcctg atggaaaacg gctcccatct ggtgctggcg
   120541 cttcttgcct ggcgagcacc ggaactggtg gaaataggta tcgctaccga cgccatcttc
   120601 gccgtcattg tgatggtgtt actggcaaga aaaatatggc gtacccacgg cacgctggac
   120661 gtgaacaact tgaccgcgct gaagggataa tgagatgagt tattctgtga tgttcgcttt
   120721 actcctgctc acgccgctgc ttttttcgct gctctgtttt gcctgccgga aacgggggct
   120781 ctctgcgact tgtacggtga ctgtattaca tagcttaggg atcacactgc tgctgattct
   120841 ggcactctgg gtggtccaaa ctgccgctga tgcaggagaa atattcgctg cgggactgtg
   120901 gcttcatatt gatggtctgg gcggtttgtt cctcgccatt cttggtgtga ttggctttct
   120961 caccggtgtt tactcgattg gctacatgcg tcatgaagtg gcacacggcg agctttcacc
   121021 cgttacactg tgcgattact acggtttctt ccatctgttt tttttcacca tgctgctggt
   121081 tgttaccagc aataacctga ttgtgatgtg ggcggcgatc gaagccacca ccttaagctc
   121141 ggcgtttctg gtaggcattt acggtcagcg ttcatcgctg gaatcagcat ggaagtacat
   121201 cattatttgt actgttggtg tcgcttttgg tctgttcggt accgtgctgg tatacgccaa
   121261 cgccgccagc gttatgccgc aggcagaaat ggcgatattc tggagcgagg ttcttaagca
   121321 atcgtccttg cttgacccaa cattaatgct gttggccttt gtgtttgtgc taattggctt
   121381 tggtaccaaa accgggctat ttcccatgca cgcctggctg ccggatgctc acagtgaagc
   121441 gccgagtccg gtcagcgccc tgctctccgc cgtattgctg aactgcgcgc tgttggtgct
   121501 gattcgctat tacatcatta tttgccaggc catcggcagc gatttcccca accggttgtt
   121561 gctcatcttc ggcatgttgt cggttgccgt ggcggcattt ttcattctgg tacagcggga
   121621 cattaagcgt ctgctggcgt actccagcgt ggagaacatg gggctggtcg cggtggcgct
   121681 aggcattggc gggccgctgg gaatttttgc cgcgctgctg cacaccttaa accacagtct
   121741 ggcaaaaacg ctgctgttct gcggttccgg caatgtactg ctcaagtacg gcacgcgcga
   121801 tctcaacgtc gtctgtggga tgctcaaaat catgccattt accgccgtgc tgtttggcgg
   121861 cggtgcgctg gcgctggcag ggatgccgcc cttcaacatt tttcttagcg aatttatgac
   121921 cgttaccgca ggactggcac gtaatcacct gctgattatc gtcctgctgt tattgctgtt
   121981 aacgctggtg ctcgcgggcc tggtacggat ggctgcgcgg gtgttaatgg cgaaaccgcc
   122041 gcaggccgtt aaccggggtg atctcggctg gttgaccacc tcgccaatgg tgattctgct
   122101 agtcatgatg ctggcgatgg gaacgcatat tccacaacct gtcatcagga tcctggcggg
   122161 cgcttccact atagtcctct cagggacgca cgacctgcct gcacaacgta gcacctggca
   122221 tgattttttg ccttcaggca ccgcatctgt ttcggagaaa cacagtgaac gttaattcat
   122281 cgtcaaatcg tggcgaagcg attctcgccg ccctgaaaac gcagttcccc ggcgcggtgc
   122341 tggatgaaga gcgacaaacg cctgaacagg tcaccattac ggtgaaaatc aatctgctgc
   122401 ctgacgttgt acattatctt tattatcaac atgatggctg gcttccagtc ctgtttggca
   122461 acgacgagcg gacacttaac ggtcattacg cggtttatta tgccctttca atggaagggg
   122521 ccgaaaaatg ctggatcgtg gtgaaggcac tggtcgatgc cgacagtcgg gagtttccgt
   122581 cagtcacacc gcgcgtccct gccgcggtct ggggcgagcg agaaattcgc gatatgtacg
   122641 ggctgattcc ggttggcctg ccggatcagc gtcgcctggt gttgcccgat gactggccgg
   122701 aagatatgca tccgctgcgc aaagatgcga tggattatcg actgcgccct gaaccgacga
   122761 cagattccga aacgtatgcg tttatcaatg agggcaacag cgatgcgcgg gtgatccctg
   122821 tcggcccgct gcatatcacc tcctatgaac cgggtcactt ccgcttgttt gtggatggcg
   122881 agcaaattgt cgatgctgat taccgcctgt tttatgtcca tcgcggcatg gagaaactgg
   122941 cagaaacgcg gatgggctac aacgaagtga ccttcttatc ggaccgcgtg tgtgggattt
   123001 gcggttttgc ccacagtgtg gcctatacca actcggttga aaatgcactg gggattgagg
   123061 tgccgcaacg agcacatact attcgctcga ttctgctgga agtcgagcgg ctacacagtc
   123121 atttgctcaa ccttggcctc tcctgccatt ttgttggttt tgataccggc tttatgcaat
   123181 ttttccgcgt gcgggaaaag tcgatgacga tggcggaatt gctgaccggg tcgcgtaaaa
   123241 cctacggtct gaatctgatt ggtggtgttc gccgcgatat tctcaaagag caacgtctgc
   123301 aaacgctgaa actggtgcgc gagatgcgct ccgacgtgtc ggagctggta gagatgctgc
   123361 ttgctacgcc gaatatggaa caacgcactc agggcattgg cattctcgac cgacaaatcg
   123421 cccgtgatta tagccctgta gggccgctga tccgcgcagt ggttttgccc gtgatttgcg
   123481 ctttgatcac ccctacgccg actacggcaa tattccaaaa acactgttta cctttaccgg
   123541 cggcgatgtt ttctcccgcg tgatggtccg tgtcaaagag acgtttgatt cgctggcaat
   123601 gctggaattt gccctcgaca acatgccgga taccccactg ctgaccgaag gctttagcta
   123661 taaacctcac gcattcgcgc tgggctttgt tgaagcgcca cgcggtgaag acgtgcactg
   123721 gagcatgctc ggtgataacc aaaaattgtt ccgctggcgc tgtcgtgccg ccacctacgc
   123781 caactggccg gtgttgcgtt acatgctgcg cggcaatacc gtttctgacg caccgctgat
   123841 tatcggtagc cttgatccct gctactcctg taccgaccgt gtgacgctgg tagatgtgcg
   123901 caagcgccag tcaaaaaccg tgctgtataa agagatcgaa cgctacggca ttgatcgtaa
   123961 ccgttcgccg ctgaagtaag gacagaagat gctgaagtta ctgaaaacta ttatgcgcgc
   124021 cggaaccccg acggtgaaat atcccttcgc gccactggag gtcagcccag gctttcgcgg
   124081 gaaaccggac ctgatgccca gccaatgtat tgcctgcggt gcctgcgcct gtgcttgtcc
   124141 ggcaaatgcg ctgactatcc agaccgacga ccagcaaaat tcgcgcacct ggcagctcta
   124201 tctggggcgt tgtatttact gcggacgttg tgaagaagtg tgcccgacca gagccatcca
   124261 gcttaccaat aactttgaac tgaccgtcac ccataaagcc gatctctata cccacgcgac
   124321 gttccatcta caacgttgca gccgttgcga acgcccgttt gccccgcaaa aaaccgtcgc
   124381 actggctgct gaattgttgg cacagcaaca aaatgctcca caaaaccgcg aaatgttatg
   124441 ggcgcaagcg agcgtctgcc cggagtgcaa acaacgcgcg acgctgatca acgacgatac
   124501 agatgtaccg ctggtggcta aggagcagct atgagtccag tgcttacgca atatgtcagc
   124561 cagcccatca cgctggacga gcaaacgcaa aagatgaagc ggcatttgct acaggatatc
   124621 cgtcgctcgg cttacgttta tcgcatcgat tgcggcggct gcaacgcctg tgaaatcgaa
   124681 atttttgctg ccatcacccc cgtattcgac gcagaacgtt ttggcattaa ggttgtttca
   124741 tcaccgcgtc acgccgatat tttgttattt actggcgcag tcacccgggc gatgcgtatg
   124801 cctgcacttc gggcgtatga gtctgccccc gatcataaaa tctgtgtttc ctacggcgcg
   124861 tgcggtgtcg gcggcggtat tttccacgat ctctacagcg tctggggcgg cagcgacacc
   124921 attgtcccca ttgatgtttg gatccccggc tgcccgccaa caccggccgc caccattcac
   124981 ggtttcgccg tggcgctcgg tttgctgcaa cagaagattc acgcggtgga ttatcgcgat
   125041 cccaccgggg tgactatgca accgttgtgg ccgcagatcc cgccatcaca gcgtatcgcc
   125101 attgagcgag aagcgcggcg gctggcgggc tatcgtcagg ggcgagaaat ttgcgatcgg
   125161 ctcctgcgcc atttaagcga cgatcctaca ggaaatcggg ttaacacctg gttgcgcgat
   125221 gccgacgatc cacgtctcaa tagtatcgtt cagcaactct ttcgcgtact ccgggggtta
   125281 catgactgaa gagtgcgggg aaattgtttt ctggacgctg cgaaaaaagt ttgtcgccag
   125341 tagcgacgag atgccggaac acagctctca ggtaatgtat tactcgctgg ctatcggtca
   125401 tcacgttggc gtgattgatt gtctgaatgt cgccttccgc tgcccactga cggaatacga
   125461 agattggctt gcactggtcg aagaggagca agcccgacgt aagatgctgg gggtgatgac
   125521 ttttggtgag attgttattg acgccagcca caccgccctg ttgacccggg cattcgcgcc
   125581 actggcagat gacgcgacgt ctgtgtggca agcgcgtagc attcaattca ttcatctgtt
   125641 ggatgaaatt gtgctggaac cggctatcta tctgatggcc agaaaaattg cgtgagaagg
   125701 atttctcatt aataaggact gttgatggct atgtcagacg aagcgatgtt tgccccgccg
   125761 caaggaataa caattgaagc ggtaaacgga atgctcgcgg agcggttagc gcagaaacac
   125821 ggcaaggcgt ctttattacg cgccttcatc ccgctgccgc cgccgttcag cccggtacaa
   125881 cttattgaac tgcatgttct caaaagcaac ttctattacc gctaccatga tgatggcagc
   125941 gatgtgacgg caacaacaga gtatcagggc gagatggtcg attattcgcg tcacgccgtc
   126001 cttctcggca gtagttgaat ggcggagcta cgctttattc gtacccacgg cagccgtttt
   126061 actccccagg attgcacact gtttaactgg ctggcacgca ttatcacccc ggttctgcaa
   126121 tcatggctca atgatgaaga acagcaggtg gcgctgcatt tgctggagaa agatcgcgat
   126181 catcatcggg tactggtgga tatcactaat gcagtgctgt cacatcttga tctcgacgat
   126241 ctgatcgctg acgtcgctcg tgagatccat cattttttcg gtctggcttc agtcagtatg
   126301 gtactgggcg atcatcgaaa gaacgagaag ttcagcctgt ggtgcagcga tctttctgcc
   126361 tcacattgtg cgtgtctgcc acacaatatg cctggcgaca gtgtattgct gacacaaacg
   126421 ctacaaaccc gacaaccgac cttgacgcac cgtgcagatg atctgtttct ctggcaacgc
   126481 gacccgttat tactcttact tgcatctaac ggctgcgaat ctgcgctcct tataccgctt
   126541 acctttggca accatacacc gggtgcattg ttgctggcgc atacctcttc cactctcttt
   126601 agtgaggaaa actgccagct actacaacac atagccgatc gcatcgctat tgccgttggc
   126661 aatgccgatg cctgccgtag aatgaccgat ttgcaggaaa gtttgcagca agaaaaccac
   126721 cagcttagcg agcagctcct ttcgaatctg ggcgtcggtg acattatcta tcaaagccag
   126781 gcaatggaag acctgctcca gcaggtagat attgtggcga agagcgacag tacggtgttg
   126841 atttgcggtg aaaccggaac cggcaaagag gtgatcgcca gagcgatcca tcaacttagc
   126901 ccgcgacacg acaagccgct ggtcaaaatc aactgcgctg ccatccccgc cagtcttctg
   126961 gaaagtgagt tattcggtca tgacaaaggg gcgtttactg gtgcgattaa tacccatcgt
   127021 ggtcgttttg aaattgccga tggcggcacg ttgtttctcg atgaaattgg cgatctgccg
   127081 ttagaacttc agcctaaact gctgcgcgta ttgcaggaac gggagattga gcgtctcggc
   127141 gggagtagaa cgatcccggt aaatgtcaga gtcattgccg ccaccaaccg tgatttgtgg
   127201 caaatggttg aagatcgcca gtttcgcagc gatctctttt atcgcctgaa tgtcttccca
   127261 ctggaattgc ctccgctgcg cgaccgtccg gaagatatcc ctcttttagc aaagcatttc
   127321 acgcaaaaaa tggcgcgcca tatgaatcgt tcaattgacg ccatcccgac cgaggcgcta
   127381 cgccagttga tgtcgtggga ctggccgggc aacgtgcgcg agctggaaaa cgtgattgag
   127441 cgagcggtac tgctgactcg cggtaacagt ctgaatttac atcttaatgt ccgacaaagc
   127501 cgtttactgc cgacgctaaa tgaagattca gcgcttcgca gttcaatggc gcagttgctg
   127561 cacccgacga cgccagagaa tgacgaagaa gaacgtcagc gcattgttca ggtattgcga
   127621 gaaaccaatg gcattgttgc cgggccccgt ggcgcggcga cacgattagg gatgaagcgc
   127681 accacgctgc tgtcacgaat gcagcgtctg ggaatctcgg ttcgcgaggt gttgtaatct
   127741 gcttttgcag gagtatgcat gagaaacaaa ctctctttcg acttgcagtt gagcgccaga
   127801 aaagcggcaa tcgctgaacg gattgccgcc cataaaattg cccgcagtaa agtgtcggtc
   127861 tttttaatgg cgatgtccgc tggcgtgttt atggcgatcg gatttacttt ttacctttcc
   127921 gttatcgccg atgccccgtc ttcataggca ttaacccatc tggtgggcgg cctttgcttt
   127981 acacttggct ttattttgct ggcggtttgc ggcaccagcc tgttcacctc gtcggtaatg
   128041 acggtaatgg caaaaagtcg gggcgttatt agttggcgaa cttggctgat taacgcactt
   128101 ctggtggcct gcggtaatct ggcaggtatt gcctgtttca gtttgttaat ctggttttcc
   128161 gggctggtga tgagtgaaaa cgcgatgtgg ggagtcgcga ttttacactg cgccgagggc
   128221 aaaatgcatc atacatttac tgaatctgtc agcctcggca ttatgtgcaa tctgatggtt
   128281 tgcctggcgc tgtggatgag ttattgcgga cgttcgttat gcgacaaaat cgtcgccatg
   128341 attttgtcca tcaccctgtt tgtcgccagt ggctttgagc actgtatcgc caatttgttt
   128401 gtgattccgt tcgccattgc cattcgccat ttcgccccta cctccttctg gcaactggcg
   128461 cacagtagcg cagacaattt tccggcactg acggtcagcc attttattac cgccaatctg
   128521 ctcccggtga tgctgggtaa tattatcggc ggtgcagtgc tggtgagtat ttgttatcgg
   128581 gctatttatt tacgtcagga atcctgaaaa atcagcccgg cgaaacagtt cgtcgggctg
   128641 aagattattc ttgcgcgatt tgcccatcgg gccaggcgtg gatcacggct ttgatcagcg
   128701 tcgccaatgg aatggcgaag aatacgcccc agaatcccca caaaccaccg aagatcacca
   128761 ccgataaaat aatcaccagc ggatgcaggt taaccgcttc ggagaacaac accggtacta
   128821 gcaggttgcc gtccagcgcc tgaataatca gatacaccgc gaagcagctc cagaattcag
   128881 tacctgcgcc aaactggaat agcgccacgc caaccaccgg aatggtcacc acaaatgcgc
   128941 caatgtacgg aataagaacc gagaagccga ccagcactgc cagcagcagc gaatagttca
   129001 gcccgaagag caagaacccc agccaggtgg cgatccccac cacgatcatc tccagcactt
   129061 tgccgcggat atagttggtg atttgttgat tcatctcctt ccacacctgt cctgccagcc
   129121 cacggttacg cggcagcacc cggcgaacgg cgttcagcat ctgctctttg tctttcagca
   129181 ggaagaagac catcaatggc accagcacca gatagacggc tatggtcagc aaaccgacca
   129241 gcgaggcgag ggaaattttc accaccgaat cgcccatggt cagcatccga ctgcgcatat
   129301 tttcggccat tgcatcaata atgcccgcgt ccattaacac cggatagcgg cgcggcaacg
   129361 tggcggcaaa gtcagaaagc ttattgagca tccccggcat atcgcggatt aagtagatgc
   129421 cctgttgcca ggcgataggc agtaccacga acgccatcag tagcaatata ccgacaaaaa
   129481 ccaccaatac aatcgacgtc gcccagcggc gggagcagcc aatagattgc aggcgcacgg
   129541 ttggccattc cagcaaataa gccagcacaa tagccaccag caacggagca agcaggccac
   129601 taaagaaaaa gataatgcca aatccggcaa ctaaaataac cagcaaggca atcgcttccg
   129661 ggtcgctaaa acggcggcga taccattgca tcaacatttc gagcataaag accttccctg
   129721 aacctcaaga gcgggattgc gatccgcaat tgtatcgaaa tgtcacaaaa aagacttcgc
   129781 tttttatgac ggattcagga aactgaaaag tcatttgagt gggctaatct tcgccgttac
   129841 actcaaaggc ggcgaggtgg gaacgatatt tcacagtatc ggtcaaatga ctatcttcag
   129901 aaatacagga tagaggttat gttcaggcag ttgaaaaaaa acctggttgc aaccctcatt
   129961 gctgctatga ccattggtca ggtagccccg gcgtttgccg acagcgcaga caccttgccg
   130021 gatatgggaa cctccgcagg aagcacgctt tccattggtc aggaaatgca gatgggcgac
   130081 tattatgtcc gccagctacg cggcagcgcg ccgttaatta atgacccgct gttaacgcaa
   130141 tatattaatt cgctggggat gcgtctggtt tcgcatgcca attcggttaa gacaccgttt
   130201 catttttttc tgatcaacaa cgacgaaatt aacgcctttg ctttctttgg cggcaacgtg
   130261 gtgctgcact ctgccctgtt ccgttattcc gataacgaaa gtcaactggc ttcagttatg
   130321 gcgcacgaaa tctcccacgt cacccaacgt cacctggcgc gagcgatgga agatcaacaa
   130381 cgcagcgcgc cgctgacctg ggtcggcgcg ttaggttcta ttttactggc gatggccagt
   130441 ccgcaggcgg ggatggcggc gctgaccggt acactggcgg gaacgcgcca ggggatgatc
   130501 agtttcaccc agcaaaatga acaggaagcg gaccgcattg gtattcaggt gctgcaacgc
   130561 tcgggattcg atccacaggc gatgccgacc ttcctggaaa aattgctcga tcaggcgcgt
   130621 tactcctcgc gcccgccgga aattttattg actcacccgt tgccggaaag tcgtctggca
   130681 gatgcccgca accgtgctaa tcagatgcgc ccgatggtgg tgcagtcgtc ggaagatttc
   130741 tatctggcga aagcgcgcac actggggatg tataattccg gacgtaacca gctcaccagt
   130801 aatttgctgg atgaatgggc gaaaggaaac gttcttcagc aacgagcggc gcaatatggt
   130861 cgtgctttac aggcgatgaa agccaataaa tacgacgagg cgcgtaaaac gctgcaaccg
   130921 ttactggcgg cagaacctgg caacgcatgg tatctcgatc tggctaccga tatcgatctt
   130981 gagcaaaaca aagccaatga ggcaatcaat cgcctgaaaa atgcccgcga tttacgcacc
   131041 aatccggtgt tgcagctcaa cctggcgaac gcttatctgc aaggcggtca accacaagaa
   131101 gcggccaata ttctgaatcg ctacaccttt aataataaag atgacagcaa cggctgggat
   131161 ttgctggcac aggcggaagc cgcgctaaat aaccgcgatc aggagctggc tgcgcgagca
   131221 gaaggttatg cgctcgccgg acgactcgat caggccattt cgctgttgag tagcgccagt
   131281 tcgcaggtga aattaggcag cctgcaacaa gcgcgttacg atgcgcgcat cgaccagttg
   131341 cgccagctgc aggaacgctt taagccttat accaagatgt aattcgcatt aaaggaagaa
   131401 caatgaccaa acaggtaaaa atctaccata acccacgctg ttcaaagagc cgggaaacgc
   131461 tgaacctgct gaaagaaaac ggcgtggagc cagaagtggt gctctacctt gagacacccg
   131521 ccgatgcggc aacgctgcgc gatttgctga aaatgctggg gatgaatagc gcccgtgaat
   131581 tgatgcgcca gaaagaggat ctttataaag aactaaacct ggctgacagt tcgcttagcg
   131641 aagaggcgct gattcaggca atggtggata atccgaagct gatggagcgc ccgattatgg
   131701 tcgcgaatgg caaagcgcgg attggtcgtc caccggagca ggtactggaa atcgtgggtt
   131761 aagatgtgct ccatcgctag ttgaagcaca ttgccggatg cgacgcttgc cacgtcttat
   131821 ccggcctacg aatactatgg attaacttcg cgtaggtcag gcgttcgcgc cgcatccgac
   131881 aataaacacc ttatctacaa cttcagaatt tctttcacaa acggaatggt cagcttacgt
   131941 tgcgcggtaa tcgacgcacg atccaactga tccaacgtca taaatagcgt gcgcatttct
   132001 ctgtcgagcc gcttcagcaa gaaacgcccc acatcttccg gcagttcaaa accacgcaaa
   132061 cgcgcgcgta actgtagcgc ctgcaactta tcttcatcag aaagtggctg caatttgtag
   132121 atctgccccc agtcgagacg cgacgcgaga tccggtaatc ccagattcaa ctgccgcggt
   132181 ggacgatcgc cggtgatcaa caaccgtgtt ttgcccgatt ccagaattcg attgtagaga
   132241 tcgaaaatcg ccatctccca caactcatcg cctgcaatac actcaatgtt gtcgatacag
   132301 accagcgaca aatgctccat accgtcgagc acttccggaa caaaccaggt gcgtttatcc
   132361 agcgggacat agcccaccgc atcgccacgc tgcgacaatt ccgcgcaagc cgcgtgcagc
   132421 agatggctgc gccccgcgcc ttcgcgtgcc cagagataga tgtaaccgct atgttcctga
   132481 cgcagcacgt tttgcagcgc ggccagtaaa gaggagttat cccccggcca gaaacttgca
   132541 aaggtttcgt cgtcaggaag ataaagtggc aaagagagct gtgccggtgt gttcagagat
   132601 acctcaacca ggatttcaca aaatcgcgag aagtttacca cagaatccca tgatgtttga
   132661 accgggcagc aacactgccc ggtcggtaca ttatttgtct gttatatccg cgtcttctgc
   132721 gtccagcacc acttcttcag ggcgcagcac actgatcagt ttgaaaatca aacttaagcc
   132781 gataccgacg atggtcgcca gcgccatacc ttttaactcc gccgcgccga tgtttacctt
   132841 cgcgccactg acgccgatga tcaaaatcac ggaagtcagg atcaggttct gtgctttgtt
   132901 gtaatccact ttcgattcga tcaaaacacg aataccggaa gcaccaatga caccataaag
   132961 cagcagcgaa acgccgccca tcaccggcaa tgggatcatc tggatagcgg cagccagttt
   133021 accgacgcag gaaagcagga tagcgaaaat cgccgccccg ccgataaccc aggtactgta
   133081 aacacgggtg atcgccatca cgccaatgtt ttctccgtaa gtggtatttg gcgtagagcc
   133141 aaagaagccg gagatcacgg tcgacaggcc gttagcaaac atcgaacggt gcagacctgg
   133201 atcgcgcagc agatcttttt tgacgatatt agccgttact accaggtgcc ctacgtgttc
   133261 ggcaataacc actaacgccg ctggcagaat agtcagaatg gcaaaccact cgaagcgcgg
   133321 cgtatagagg gttggcagtg caaaccagtg agcattaata atcggcgtgg tatcgacaat
   133381 tcccattgcg aaagagagcg cgtaccccac cagcacgcca attaaaatcg ggataattgc
   133441 caggaaacca cgaaacagca cggaacctaa aaccgtgacc gccagggtgg taatagagat
   133501 gatgatggtt ttggagtttg gcgtttgccc ttcagccggg agtaaacccg ccataccggc
   133561 agctacgccc gccagctcca gaccgatgac ggcaacgatt gcgcccattg ccgcaggtgg
   133621 aaacagcacg tccaaccagc cggtccccgc tttcttcacg ataaaagaaa ccaggcagaa
   133681 cagcacgccg cacataataa agccgcccag cgcgacttca taccctaacg gcaacagtaa
   133741 caataccggt gaaataaagg caaagctgga accaagataa gccggaattt tccctttaca
   133801 gatgaagaga tacagcagcg ttccaatacc gttaaataac agtacagtcg ccgggttaat
   133861 atgaaataag acgggcacca ggacggttgc accaaacatg gcgaacaaat gttgcaaact
   133921 aagcgggatt gtctgtaaaa gtggcggtct ttcactcacc ccgatagcac ggcgcgtcat
   133981 agtattatcc tctgtattat gtgttatagg cgctttactc aaaaaaaagc cgactcttaa
   134041 agtcggcttt aattattttt attctttatt tcgtaccaaa gattttgtca ccggcatcgc
   134101 cgaggcccgg aataatgtat ccgtgctcgt tcagtccctg gtcaatcgat gcagtataca
   134161 gttcgacgtc cgggtgcgct ttttccagcg cagcgatacc ttctggcgca gcgaccagca
   134221 ccagaacttt gatgctgctg cagcccgctt ttttcagcag gtcgatggtc gcgataacgg
   134281 aaccaccggt tgccagcatt gggtcaacga tcagcgccat acgctcatcg atgttagaaa
   134341 ccagtttctg gaagtacggt accggctcca gcgtttcttc attacggtac ataccgacaa
   134401 cgctgatgcg cgcgctcgga acgttttcca gcacaccgtc catcatacca agacccgcac
   134461 gcagaattgg cacaacggta attttcttac ctttgatctg gtcgatttct accgggccgt
   134521 tccagccttc gatagttact ttttccgttt cgaggtcggc ggtcgcttcg taagtcagca
   134581 ggctacccac ttcggaagcg agttcgcgaa agcgcttggt gctgatatct tgctcacgca
   134641 tcagtcccag cttgtgtttg acgagtgggt gtttgacttc cacgatcttc atactctttc
   134701 tcctttgagg ggcagccaca aaaaaaatcg aaggattata cctcctttct tcaaggcggc
   134761 aatattattt tcgttgactt tagtcaaaat gataacagtt tgagataaag ttattttata
   134821 ttcaaatggt tatgaaagaa gattattcca tccgaaaact aacctttgcc ctggcacaag
   134881 tcttctttcg ccgcgcgccc ggggaaaaga cgtgcaaaaa ggttgtgtaa aacagtctcg
   134941 caaacgtttg ctttccctgt tagaattgcg ccgaatttta tttttctacc gcaagtaacg
   135001 cgtggggacc caagcagtga ccgataaaac ctctcttagc tacaaagatg ccggtgttga
   135061 tattgacgcg ggtaatgctc tggttggaag aatcaaaggc gtagtgaaga aaacgcgtcg
   135121 tccggaagtg atgggcggtc tgggcggctt cggtgcgctg tgtgcattgc cgcaaaaata
   135181 tcgtgaaccc gtgctggttt ccggcactga cggcgtaggt accaagctgc gtctggcaat
   135241 ggacttaaaa cgtcacgaca ccattggtat tgatctggtc gccatgtgcg ttaatgacct
   135301 ggtggtgcaa ggtgcggaac cgctgttttt cctcgactat tacgcaaccg gaaaactgga
   135361 tgttgatacc gcttcagcag tgatcagcgg catcgcggaa ggttgtctgc aatcgggctg
   135421 ttctctggtg ggtggcgaaa cggcagaaat gccggggatg tatcacggtg aagattacga
   135481 tgtcgcgggt ttctgcgtgg gcgtggtaga aaaatcagaa atcatcgacg gctctaaagt
   135541 cagcgacggc gatgtgctga ttgcactcgg ttccagcggt ccgcactcga acggctattc
   135601 gctggtgcgc aaaattcttg aagtcagcgg ttgtgatccg caaaccaccg aacttgatgg
   135661 taagccatta gccgatcatc tgctggcacc gacccgcatt tacgtgaagt cagtgctgga
   135721 gttgattgaa aaggtcgatg tgcatgccat tgcgcacctg accggcggcg gcttctggga
   135781 aaacattccg cgcgtattgc cagataatac ccaggcagtg attgatgaat cttcctggca
   135841 gtggccggaa gtgttcaact ggctgcaaac ggcaggtaac gttgagcgcc atgaaatgta
   135901 tcgcaccttc aactgcggcg tcgggatgat tatcgccctg cctgctccgg aagtggacaa
   135961 agccctcgcc ctgctaaatg ccaacggtga aaacgcgtgg aaaatcggta tcatcaaagc
   136021 ctctgattcc gaacaacgcg tggttatcga ataatgaata ttgtggtgct tatttccggc
   136081 aacggaagta atttacaggc aattattgac gcctgtaaaa ccaacaaaat taaaggcacc
   136141 gtacgggcag ttttcagcaa taaggccgac gcgttcggcc ttgaacgcgc ccgccaggcg
   136201 ggtattgcaa cgcatacgct catcgccagc gcgtttgaca gtcgtgaagc ctatgaccgg
   136261 gagttgattc atgaaatcga catgtacgca cccgatgtgg tcgtgctggc tggttttatg
   136321 cgcattctca gcccggcgtt tgtctcccac tatgccgggc gtttgctgaa cattcaccct
   136381 tctctgctgc cgaaatatcc cggattacac acccatcgtc aggcgctgga aaatggcgat
   136441 gaagagcacg gtacatcggt gcatttcgtc accgatgaac tggacggtgg cccggttatt
   136501 ttacaggcga aagtcccggt atttgctggt gatacggaag atgacatcac cgcccgcgtg
   136561 caaacccagg aacacgctat ttatccactg gtgattagct ggtttgccga tggtcgtctg
   136621 aaaatgcacg aaaacgccgc gtggctggat ggtcaacgtc tgccgccgca gagctacgct
   136681 gccgacgagt aatgcccccg tagttaaagc gccagctctg ccgctggcgt ttttcaattc
   136741 acctgtaaat cgcaagctcc agcagttttt ttcccccttt tctggcatag ttggacatct
   136801 gccaatattg ctcgccataa tatccaggca gtgtcccgtg aataaaacgg agtaaaagtg
   136861 gtaatgggtc aggaaaagct atacatcgaa aaagagctca gttggttatc gttcaatgaa
   136921 cgcgtgcttc aggaagcggc ggacaaatct aacccgctga ttgaaaggat gcgtttcctg
   136981 gggatctatt ccaataacct tgatgagttc tataaagtcc gcttcgctga actgaagcga
   137041 cgcatcatta ttagcgaaga acaaggctcc aacgctcatt cccgccattt actgggcaaa
   137101 attcagtccc gggtgctgaa agccgatcag gaattcgacg gcctctacaa cgagctattg
   137161 ctggagatgg cgcgcaacca gatcttcctg attaatgaac gccagctctc cgtcaatcaa
   137221 caaaactggc tgcgtcatta ttttaagcag tatctgcgtc agcacattac gccgatttta
   137281 atcaatcctg acactgactt agtgcagttc ctgaaagatg attacaccta tctggcggtg
   137341 gaaattatcc gtagcgatag catccgttac gcgctgctgg agatcccatc agataaagtg
   137401 ccgcgctttg tgaatttacc gccagaagcg ccgcgtcgac gcaagccgat gattcttctg
   137461 gataacattc tgcgttactg ccttgatgat attttcaaag gcttctttga ttatgacgcg
   137521 ctgaatgcct attcaatgaa gatgacccgc gatgccgaat acgatttagt gcatgagatg
   137581 gaagccagcc tgatggagtt gatgtcttcc agtctcaagc agcgtttaac tgctgagccg
   137641 gtgcgttttg tttatcagcg cgatatgccc aatgcgctgg ttgaagtgtt acgcgaaaaa
   137701 ctgactattt cccgctacga ctccatcgtc cccggcggtc gttatcataa ttttaaagac
   137761 tttattaatt ttcccaatgt cggcaaagcc aatctggtga acaaaccact gccgcgttta
   137821 cgccatattt ggtttgataa agcccagttc cgcaatggtt ttgatgccat tcgcgaacgc
   137881 gatgtgttgc tctattatcc ttatcacacc tttgggcatg tgctggaact gctgcgtcag
   137941 gcttcgttcg acccgagcgt actggcgatt aaaattaaca tttaccgcgt ggcgaaagat
   138001 tcacgcatca tcgactcgat gatccacgcc gcacataacg gtaagaaagt cactgtggtg
   138061 gttgagttac aggcgcgttt cgacgaagaa gccaacattc actgggcgaa gcgcctgacc
   138121 gaagcaggcg tgcacgttat cttctctgcg ccggggctga aaattcacgc caaactgttc
   138181 ctgatttcac gtaaagaaaa cggtgaagtg gtccgttacg cacacatcgg gaccgggaac
   138241 tttaacgaaa aaaccgcgcg tctttatact gactattcgt tgctgaccgc agatgcgcgt
   138301 atcaccaacg aagtacggcg ggtatttaac tttattgaaa acccataccg cccggtgaca
   138361 tttgattatt taatggtgtc accgcaaaac tctcgccgtc tgttatatga aatggtagac
   138421 cgcgaaatcg ccaacgcgca gcaagggctg cccagtggta tcaccctgaa gctaaataac
   138481 cttgtcgata aaggcctggt tgatcgtctg tatgcggcct ccagctccgg cgtaccggtt
   138541 aatctgctgg ttcgcggaat gtgttcgctg atccccaatc tggaaggcat tagcgacaac
   138601 attcgtgcca tcagtattgt tgaccgttac cttgaacatg accgggttta tatttttgaa
   138661 aatggcggcg ataaaaaggt ctacctttct tccgccgact ggatgacgcg caatattgat
   138721 tatcgtattg aagtggcgac accgctgctc gatccgcgcc tgaagcagcg ggtgctggac
   138781 atcatcgaca tattgttcag cgatacggtc aaagcacgtt atatcgataa agaactcagt
   138841 aatcgctacg ttccccgcgg caatcgccgc aaagtacggg cgcagttggc gatttacgac
   138901 tacatcaaat cactcgaaca acctgaataa ccctatgcca atacacgata aatcccctcg
   138961 tccgcaggag tttgctgcgg tcgatcttgg ttcaaacagt tttcacatgg tcatagcccg
   139021 tgtggtagat ggtgccatgc agattattgg ccgcctgaaa cagcgggtgc atctggcgga
   139081 cggcctgggg ccagataata tgttgagtga agaggcaatg acgcgcggtt tgaactgtct
   139141 gtcgctgttt gccgaacggc tacaagggtt ttctcctgcc agcgtctgta tagtcggcac
   139201 ccatacgctg cgtcaggcgc tgaacgccac tgactttctg aaacgcgcgg aaaaggtcat
   139261 tccctacccg attgaaatta tttccggtaa tgaagaagcc cgtctgattt ttatgggcgt
   139321 ggaacatacc caaccggaaa aaggtcgcaa actggttatt gatattggcg gcggatctac
   139381 ggaactggtg attggtgaaa atttcgaacc tattctcgtt gaaagccgcc ggatgggttg
   139441 tgtcagcttt gcccagcttt attttcctgg cggggtcatc aataaagaga attttcagcg
   139501 cgctcgcatg gcggcagcac aaaaactgga aactttaacc tggcaattcc gtattcaggg
   139561 ctggaacgtt gcaatgggcg cttccggtac cataaaagcc gcccatgaag tgttaatgga
   139621 aatgggcgag aaagacggga taattacccc ggaacgtctg gaaaaactgg taaaagaaat
   139681 tttacgtcac cgtaatttcg catcgctgag tttaccgggt ctttccgaag agcggaaaac
   139741 agtcttcgtt cctggactgg cgattttatg tggtgtgttt gatgctttag ccatccgtga
   139801 actgcgcctt tctgacgggg cgttacgcga aggcgtactg tatgaaatgg aaggacgttt
   139861 ccgtcatcag gatgtgcgta gtcgcaccgc cagcagcctc gccaaccagt atcacataga
   139921 cagcgaacag gcccgacgag tgctggatac cactatgcaa atgtacgaac agtggcggga
   139981 acagcaaccg aagctggcgc atccgcaact ggaggcgcta ctgcgatggg ccgccatgct
   140041 gcatgaggtc gggttgaata tcaaccacag cggtttgcat cgccactccg cttatattct
   140101 gcaaaacagt gacttgccgg gttttaatca ggaacagcag ctgatgatgg cgacactgct
   140161 gcgctatcac cgtaaagcga ttaagctcga cgatctgccg cgctttacct tgtttaagaa
   140221 gaaacagttc ctgccactga tacagctatt gcgccttggc gtattactaa acaatcaacg
   140281 tcaggcaacc accacaccgt caacattgac actgattact gatgacagtc actggacact
   140341 gcgtttcccg catgactggt ttagtcagaa tgcgctggta ctgcttgatc tggaaaagga
   140401 gcaagaatac tgggaaggcg tggctggctg gcggttgaaa attgaagaag aaagtacccc
   140461 agaaatcgcc gcttaatgct ttgcgggccg acgagaaatg tcggcccgca ttattcaggc
   140521 actttcgcga atgggttcga tttcattcag cgtatcaatt aacggttgcg gcttaccaat
   140581 aagataaccc tgcatataat cgatccccaa agagagcacc gcctcgcgga tctcttcgtt
   140641 ttcaacgtac tctgccacta cccgcatttt cttcattcgc gccagatggc aaatcgacgc
   140701 cacaatctga taatccagac tatttgacac aatattgcgg ataaagctgc cgtcaatttt
   140761 aagcagatcg gcattcacat ttttaagccg tgcatagctg gcgtagccgg tgccgaaatc
   140821 atcaatcgca atctggcagc ctaattcctg aagatgctgc aaggtgattt gcgcctgctt
   140881 aacattggtc agagcattac tttcggtgac ttcaaaaata agttgccacg cttcaatctg
   140941 atatttagcc agcaactgac tgacttcaac aggaaaacga gcctgacata ccgaggttgg
   141001 agacagatta atagcaaaac ggtgagcggg catcttcgct ctgttttcag ccataaattg
   141061 cagcgtatgc tcaatgaccc acatgtcgat actcgacgat aaaccaaatt cgtgcgcgac
   141121 cggcaagaag ctatcggggc cgatcagttc atcattctca cctttcatgc gcagaagaat
   141181 ttcatggtaa acatcaccac gcataccggt aatcggctgg gccatcagga aaaaatggtt
   141241 gtgctccagc gcctgctgta gtcgattcat catcgcgact ttatctttca attcgcgttg
   141301 caaatacatt gccccgcaac gctgcatatt ttccggggcg ttggtcacga tggaaagttc
   141361 ggcgaccgta tttagctctc ccagcagcag gtagatatga ttcactggcg agcgcacata
   141421 gcagtaactg acgccaatct gcggttgcat cggcatccca tcccaaaaga aacgaaattg
   141481 cttgagatga ctatccagtg cggtaatgcg ctcctggtgc gattctgtat tcagtcgcag
   141541 cgcgagatcg ttacccgaaa gctgataaac atcttcaccc ggttccagca agggtgacag
   141601 ccagtgagaa agt
//
LOCUS       DAJVQH010000002       111713 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJVQH010000002 DAJVQH010000000
VERSION     DAJVQH010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 111713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 111713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..111713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.002"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            596..2881
                     /gene="nrdA"
                     /locus_tag="OQM87_000134"
     CDS             596..2881
                     /gene="nrdA"
                     /locus_tag="OQM87_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCS1506414.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3115..4245
                     /gene="nrdB"
                     /locus_tag="OQM87_000135"
     CDS             3115..4245
                     /gene="nrdB"
                     /locus_tag="OQM87_000135"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCS1506415.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4245..4499
                     /gene="yfaE"
                     /locus_tag="OQM87_000136"
     CDS             4245..4499
                     /gene="yfaE"
                     /locus_tag="OQM87_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCS1506416.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4553..5203)
                     /gene="inaA"
                     /locus_tag="OQM87_000137"
     CDS             complement(4553..5203)
                     /gene="inaA"
                     /locus_tag="OQM87_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCS1506417.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5367..5609
                     /locus_tag="OQM87_000138"
                     /pseudo
     CDS             5367..5609
                     /locus_tag="OQM87_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5651..6727)
                     /gene="glpQ"
                     /locus_tag="OQM87_000139"
     CDS             complement(5651..6727)
                     /gene="glpQ"
                     /locus_tag="OQM87_000139"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1506418.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6732..8090)
                     /gene="glpT"
                     /locus_tag="OQM87_000140"
     CDS             complement(6732..8090)
                     /gene="glpT"
                     /locus_tag="OQM87_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCS1506419.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8363..9991
                     /gene="glpA"
                     /locus_tag="OQM87_000141"
     CDS             8363..9991
                     /gene="glpA"
                     /locus_tag="OQM87_000141"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCS1506420.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9981..11240
                     /gene="glpB"
                     /locus_tag="OQM87_000142"
     CDS             9981..11240
                     /gene="glpB"
                     /locus_tag="OQM87_000142"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCS1506421.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11237..12427
                     /gene="glpC"
                     /locus_tag="OQM87_000143"
     CDS             11237..12427
                     /gene="glpC"
                     /locus_tag="OQM87_000143"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCS1506422.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12620..13519
                     /locus_tag="OQM87_000144"
     CDS             12620..13519
                     /locus_tag="OQM87_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS1506423.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            13523..13759
                     /locus_tag="OQM87_000145"
                     /pseudo
     CDS             13523..13759
                     /locus_tag="OQM87_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13800..14270)
                     /locus_tag="OQM87_000146"
     CDS             complement(13800..14270)
                     /locus_tag="OQM87_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS1506424.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14325..15527)
                     /locus_tag="OQM87_000147"
     CDS             complement(14325..15527)
                     /locus_tag="OQM87_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCS1506425.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSSRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15614..16156)
                     /locus_tag="OQM87_000148"
     CDS             complement(15614..16156)
                     /locus_tag="OQM87_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506426.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16435..16860
                     /gene="nudI"
                     /locus_tag="OQM87_000149"
     CDS             16435..16860
                     /gene="nudI"
                     /locus_tag="OQM87_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCS1506427.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16899..17501)
                     /locus_tag="OQM87_000150"
     CDS             complement(16899..17501)
                     /locus_tag="OQM87_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCS1506428.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNMVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17809..18948
                     /gene="arnB"
                     /locus_tag="OQM87_000151"
     CDS             17809..18948
                     /gene="arnB"
                     /locus_tag="OQM87_000151"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCS1506429.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18952..19920
                     /gene="arnC"
                     /locus_tag="OQM87_000152"
     CDS             18952..19920
                     /gene="arnC"
                     /locus_tag="OQM87_000152"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCS1506430.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19920..21902
                     /gene="arnA"
                     /locus_tag="OQM87_000153"
     CDS             19920..21902
                     /gene="arnA"
                     /locus_tag="OQM87_000153"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCS1506431.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDFVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21899..22789
                     /locus_tag="OQM87_000154"
     CDS             21899..22789
                     /locus_tag="OQM87_000154"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCS1506432.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22789..24441
                     /gene="arnT"
                     /locus_tag="OQM87_000155"
     CDS             22789..24441
                     /gene="arnT"
                     /locus_tag="OQM87_000155"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCS1506433.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDLWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24438..24773
                     /gene="arnE"
                     /locus_tag="OQM87_000156"
     CDS             24438..24773
                     /gene="arnE"
                     /locus_tag="OQM87_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCS1506434.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24773..25159
                     /gene="arnF"
                     /locus_tag="OQM87_000157"
     CDS             24773..25159
                     /gene="arnF"
                     /locus_tag="OQM87_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCS1506435.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25153..25425)
                     /gene="pmrD"
                     /locus_tag="OQM87_000158"
     CDS             complement(25153..25425)
                     /gene="pmrD"
                     /locus_tag="OQM87_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCS1506436.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25530..26885)
                     /gene="menE"
                     /locus_tag="OQM87_000159"
     CDS             complement(25530..26885)
                     /gene="menE"
                     /locus_tag="OQM87_000159"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCS1506437.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADVLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26882..27844)
                     /gene="menC"
                     /locus_tag="OQM87_000160"
     CDS             complement(26882..27844)
                     /gene="menC"
                     /locus_tag="OQM87_000160"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCS1506438.1"
                     /translation="MRSAQVYRWQIPMDAGVLLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27844..28701)
                     /gene="menB"
                     /locus_tag="OQM87_000161"
     CDS             complement(27844..28701)
                     /gene="menB"
                     /locus_tag="OQM87_000161"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCS1506439.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28716..29474)
                     /gene="menH"
                     /locus_tag="OQM87_000162"
     CDS             complement(28716..29474)
                     /gene="menH"
                     /locus_tag="OQM87_000162"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCS1506440.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29471..31141)
                     /gene="menD"
                     /locus_tag="OQM87_000163"
     CDS             complement(29471..31141)
                     /gene="menD"
                     /locus_tag="OQM87_000163"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCS1506441.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31230..32525)
                     /gene="menF"
                     /locus_tag="OQM87_000164"
     CDS             complement(31230..32525)
                     /gene="menF"
                     /locus_tag="OQM87_000164"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCS1506442.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32604..32909)
                     /gene="elaB"
                     /locus_tag="OQM87_000165"
     CDS             complement(32604..32909)
                     /gene="elaB"
                     /locus_tag="OQM87_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCS1506443.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32964..33425)
                     /locus_tag="OQM87_000166"
     CDS             complement(32964..33425)
                     /locus_tag="OQM87_000166"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1506444.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33490..34407
                     /gene="rbn"
                     /locus_tag="OQM87_000167"
     CDS             33490..34407
                     /gene="rbn"
                     /locus_tag="OQM87_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCS1506445.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TAQRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34595..35815
                     /gene="elaD"
                     /locus_tag="OQM87_000168"
     CDS             34595..35815
                     /gene="elaD"
                     /locus_tag="OQM87_000168"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCS1506446.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGNEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLKRVI
                     NSFNSELMGKMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPKNRASGKRVS"
     gene            36235..36850
                     /locus_tag="OQM87_000169"
                     /pseudo
     CDS             36235..36850
                     /locus_tag="OQM87_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            36953..37456
                     /locus_tag="OQM87_000170"
     CDS             36953..37456
                     /locus_tag="OQM87_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCS1506447.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(37523..38980)
                     /gene="nuoN"
                     /locus_tag="OQM87_000171"
     CDS             complement(37523..38980)
                     /gene="nuoN"
                     /locus_tag="OQM87_000171"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCS1506448.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFDYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(38987..40516)
                     /gene="nuoM"
                     /locus_tag="OQM87_000172"
     CDS             complement(38987..40516)
                     /gene="nuoM"
                     /locus_tag="OQM87_000172"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCS1506449.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(40680..42521)
                     /gene="nuoL"
                     /locus_tag="OQM87_000173"
     CDS             complement(40680..42521)
                     /gene="nuoL"
                     /locus_tag="OQM87_000173"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCS1506450.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAAQV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(42518..42820)
                     /gene="nuoK"
                     /locus_tag="OQM87_000174"
     CDS             complement(42518..42820)
                     /gene="nuoK"
                     /locus_tag="OQM87_000174"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCS1506451.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(42817..43371)
                     /gene="nuoJ"
                     /locus_tag="OQM87_000175"
     CDS             complement(42817..43371)
                     /gene="nuoJ"
                     /locus_tag="OQM87_000175"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCS1506452.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(43383..43925)
                     /gene="nuoI"
                     /locus_tag="OQM87_000176"
     CDS             complement(43383..43925)
                     /gene="nuoI"
                     /locus_tag="OQM87_000176"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCS1506453.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(43940..44917)
                     /gene="nuoH"
                     /locus_tag="OQM87_000177"
     CDS             complement(43940..44917)
                     /gene="nuoH"
                     /locus_tag="OQM87_000177"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCS1506454.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(44914..47640)
                     /gene="nuoG"
                     /locus_tag="OQM87_000178"
     CDS             complement(44914..47640)
                     /gene="nuoG"
                     /locus_tag="OQM87_000178"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCS1506455.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(47693..49030)
                     /gene="nuoF"
                     /locus_tag="OQM87_000179"
     CDS             complement(47693..49030)
                     /gene="nuoF"
                     /locus_tag="OQM87_000179"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCS1506456.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(49027..49527)
                     /gene="nuoE"
                     /locus_tag="OQM87_000180"
     CDS             complement(49027..49527)
                     /gene="nuoE"
                     /locus_tag="OQM87_000180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCS1506457.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(49530..51332)
                     /gene="nuoC"
                     /locus_tag="OQM87_000181"
     CDS             complement(49530..51332)
                     /gene="nuoC"
                     /locus_tag="OQM87_000181"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCS1506458.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(51426..52088)
                     /gene="nuoB"
                     /locus_tag="OQM87_000182"
     CDS             complement(51426..52088)
                     /gene="nuoB"
                     /locus_tag="OQM87_000182"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCS1506459.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(52104..52547)
                     /gene="nuoA"
                     /locus_tag="OQM87_000183"
     CDS             complement(52104..52547)
                     /gene="nuoA"
                     /locus_tag="OQM87_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCS1506460.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(53178..54116)
                     /gene="lrhA"
                     /locus_tag="OQM87_000184"
     CDS             complement(53178..54116)
                     /gene="lrhA"
                     /locus_tag="OQM87_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCS1506461.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            55080..56297
                     /gene="alaA"
                     /locus_tag="OQM87_000185"
     CDS             55080..56297
                     /gene="alaA"
                     /locus_tag="OQM87_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCS1506462.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            56381..56980
                     /gene="yfbR"
                     /locus_tag="OQM87_000186"
     CDS             56381..56980
                     /gene="yfbR"
                     /locus_tag="OQM87_000186"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCS1506463.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(57039..58871)
                     /locus_tag="OQM87_000187"
     CDS             complement(57039..58871)
                     /locus_tag="OQM87_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCS1506464.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(58958..59608)
                     /gene="hxpA"
                     /locus_tag="OQM87_000188"
     CDS             complement(58958..59608)
                     /gene="hxpA"
                     /locus_tag="OQM87_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCS1506465.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALSGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(59619..60113)
                     /locus_tag="OQM87_000189"
     CDS             complement(59619..60113)
                     /locus_tag="OQM87_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCS1506466.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(60196..60651)
                     /locus_tag="OQM87_000190"
     CDS             complement(60196..60651)
                     /locus_tag="OQM87_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCS1506467.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            60989..62191
                     /gene="ackA"
                     /locus_tag="OQM87_000191"
     CDS             60989..62191
                     /gene="ackA"
                     /locus_tag="OQM87_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCS1506468.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            62266..64410
                     /gene="pta"
                     /locus_tag="OQM87_000192"
     CDS             62266..64410
                     /gene="pta"
                     /locus_tag="OQM87_000192"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS1506469.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            64600..66120
                     /gene="yfcC"
                     /locus_tag="OQM87_000193"
     CDS             64600..66120
                     /gene="yfcC"
                     /locus_tag="OQM87_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCS1506470.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(66153..66695)
                     /gene="yfcD"
                     /locus_tag="OQM87_000194"
     CDS             complement(66153..66695)
                     /gene="yfcD"
                     /locus_tag="OQM87_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCS1506471.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(66753..67304)
                     /gene="yfcE"
                     /locus_tag="OQM87_000195"
     CDS             complement(66753..67304)
                     /gene="yfcE"
                     /locus_tag="OQM87_000195"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCS1506472.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(67360..68004)
                     /gene="yfcF"
                     /locus_tag="OQM87_000196"
     CDS             complement(67360..68004)
                     /gene="yfcF"
                     /locus_tag="OQM87_000196"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCS1506473.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            68140..68787
                     /gene="yfcG"
                     /locus_tag="OQM87_000197"
     CDS             68140..68787
                     /gene="yfcG"
                     /locus_tag="OQM87_000197"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCS1506474.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            68844..69206
                     /gene="folX"
                     /locus_tag="OQM87_000198"
     CDS             68844..69206
                     /gene="folX"
                     /locus_tag="OQM87_000198"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCS1506475.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            69227..70120
                     /locus_tag="OQM87_000199"
     CDS             69227..70120
                     /locus_tag="OQM87_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCS1506476.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(70168..71058)
                     /gene="rpnB"
                     /locus_tag="OQM87_000200"
     CDS             complement(70168..71058)
                     /gene="rpnB"
                     /locus_tag="OQM87_000200"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCS1506477.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIA
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(71255..72028)
                     /gene="hisP"
                     /locus_tag="OQM87_000201"
     CDS             complement(71255..72028)
                     /gene="hisP"
                     /locus_tag="OQM87_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCS1506478.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEACERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(72036..72752)
                     /locus_tag="OQM87_000202"
     CDS             complement(72036..72752)
                     /locus_tag="OQM87_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1506479.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(72749..73435)
                     /gene="hisQ"
                     /locus_tag="OQM87_000203"
     CDS             complement(72749..73435)
                     /gene="hisQ"
                     /locus_tag="OQM87_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCS1506480.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(73525..74307)
                     /gene="hisJ"
                     /locus_tag="OQM87_000204"
     CDS             complement(73525..74307)
                     /gene="hisJ"
                     /locus_tag="OQM87_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCS1506481.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(74528..75310)
                     /gene="argT"
                     /locus_tag="OQM87_000205"
     CDS             complement(74528..75310)
                     /gene="argT"
                     /locus_tag="OQM87_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCS1506482.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(75576..76145)
                     /gene="ubiX"
                     /locus_tag="OQM87_000206"
     CDS             complement(75576..76145)
                     /gene="ubiX"
                     /locus_tag="OQM87_000206"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCS1506483.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(76240..77757)
                     /gene="purF"
                     /locus_tag="OQM87_000207"
     CDS             complement(76240..77757)
                     /gene="purF"
                     /locus_tag="OQM87_000207"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCS1506484.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(77794..78282)
                     /gene="cvpA"
                     /locus_tag="OQM87_000208"
     CDS             complement(77794..78282)
                     /gene="cvpA"
                     /locus_tag="OQM87_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCS1506485.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(78541..79203)
                     /gene="dedD"
                     /locus_tag="OQM87_000209"
     CDS             complement(78541..79203)
                     /gene="dedD"
                     /locus_tag="OQM87_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCS1506486.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(79193..80461)
                     /gene="folC"
                     /locus_tag="OQM87_000210"
     CDS             complement(79193..80461)
                     /gene="folC"
                     /locus_tag="OQM87_000210"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCS1506487.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(80531..81445)
                     /gene="accD"
                     /locus_tag="OQM87_000211"
     CDS             complement(80531..81445)
                     /gene="accD"
                     /locus_tag="OQM87_000211"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCS1506488.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(81601..82260)
                     /locus_tag="OQM87_000212"
     CDS             complement(81601..82260)
                     /locus_tag="OQM87_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1506489.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(82343..83155)
                     /gene="truA"
                     /locus_tag="OQM87_000213"
     CDS             complement(82343..83155)
                     /gene="truA"
                     /locus_tag="OQM87_000213"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCS1506490.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(83155..84168)
                     /locus_tag="OQM87_000214"
     CDS             complement(83155..84168)
                     /locus_tag="OQM87_000214"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1506491.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(84234..85370)
                     /gene="pdxB"
                     /locus_tag="OQM87_000215"
     CDS             complement(84234..85370)
                     /gene="pdxB"
                     /locus_tag="OQM87_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCS1506492.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            85469..86464
                     /gene="flk"
                     /locus_tag="OQM87_000216"
     CDS             85469..86464
                     /gene="flk"
                     /locus_tag="OQM87_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCS1506493.1"
                     /translation="MIQPISGPPLGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(86461..87639)
                     /locus_tag="OQM87_000217"
     CDS             complement(86461..87639)
                     /locus_tag="OQM87_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1506494.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLCFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(87914..89134)
                     /gene="fabB"
                     /locus_tag="OQM87_000218"
     CDS             complement(87914..89134)
                     /gene="fabB"
                     /locus_tag="OQM87_000218"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCS1506495.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            89293..91299
                     /gene="mnmC"
                     /locus_tag="OQM87_000219"
     CDS             89293..91299
                     /gene="mnmC"
                     /locus_tag="OQM87_000219"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCS1506496.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVLVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(91420..91698)
                     /locus_tag="OQM87_000220"
     CDS             complement(91420..91698)
                     /locus_tag="OQM87_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCS1506497.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(91732..92280)
                     /gene="epmC"
                     /locus_tag="OQM87_000221"
     CDS             complement(91732..92280)
                     /gene="epmC"
                     /locus_tag="OQM87_000221"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCS1506498.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(92280..93089)
                     /locus_tag="OQM87_000222"
     CDS             complement(92280..93089)
                     /locus_tag="OQM87_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCS1506499.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(93089..93913)
                     /gene="mepA"
                     /locus_tag="OQM87_000223"
     CDS             complement(93089..93913)
                     /gene="mepA"
                     /locus_tag="OQM87_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCS1506500.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(93917..95002)
                     /gene="aroC"
                     /locus_tag="OQM87_000224"
     CDS             complement(93917..95002)
                     /gene="aroC"
                     /locus_tag="OQM87_000224"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCS1506501.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(95037..95969)
                     /gene="prmB"
                     /locus_tag="OQM87_000225"
     CDS             complement(95037..95969)
                     /gene="prmB"
                     /locus_tag="OQM87_000225"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1506502.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            96135..96686
                     /gene="smrB"
                     /locus_tag="OQM87_000226"
     CDS             96135..96686
                     /gene="smrB"
                     /locus_tag="OQM87_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCS1506503.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(96790..97680)
                     /locus_tag="OQM87_000227"
     CDS             complement(96790..97680)
                     /locus_tag="OQM87_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCS1506504.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(97745..98191)
                     /locus_tag="OQM87_000228"
     CDS             complement(97745..98191)
                     /locus_tag="OQM87_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1506505.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNT"
     gene            complement(98188..98658)
                     /locus_tag="OQM87_000229"
     CDS             complement(98188..98658)
                     /locus_tag="OQM87_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1506506.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(98655..99203)
                     /locus_tag="OQM87_000230"
     CDS             complement(98655..99203)
                     /locus_tag="OQM87_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1506507.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(99208..99930)
                     /locus_tag="OQM87_000231"
     CDS             complement(99208..99930)
                     /locus_tag="OQM87_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1506508.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPYQYKV
                     TLTRNGQEFTVNNPTSYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSC"
     gene            complement(99950..100672)
                     /locus_tag="OQM87_000232"
     CDS             complement(99950..100672)
                     /locus_tag="OQM87_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1506509.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATFTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(100732..102591)
                     /locus_tag="OQM87_000233"
     CDS             complement(100732..102591)
                     /locus_tag="OQM87_000233"
                     /inference="COORDINATES: protein motif:HMM:NF011812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HCS1506510.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYARRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGTMAVGRTAVRSVISAVSMTRLTIS"
     gene            complement(102673..103236)
                     /locus_tag="OQM87_000234"
     CDS             complement(102673..103236)
                     /locus_tag="OQM87_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1506511.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(103920..104405)
                     /gene="sixA"
                     /locus_tag="OQM87_000235"
     CDS             complement(103920..104405)
                     /gene="sixA"
                     /locus_tag="OQM87_000235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCS1506512.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(104608..106752)
                     /gene="fadJ"
                     /locus_tag="OQM87_000236"
     CDS             complement(104608..106752)
                     /gene="fadJ"
                     /locus_tag="OQM87_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCS1506513.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(106752..108062)
                     /gene="fadI"
                     /locus_tag="OQM87_000237"
     CDS             complement(106752..108062)
                     /gene="fadI"
                     /locus_tag="OQM87_000237"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCS1506514.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(108242..108526)
                     /locus_tag="OQM87_000238"
     CDS             complement(108242..108526)
                     /locus_tag="OQM87_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCS1506515.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            108898..110238
                     /gene="fadL"
                     /locus_tag="OQM87_000239"
     CDS             108898..110238
                     /gene="fadL"
                     /locus_tag="OQM87_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCS1506516.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            110604..111662
                     /locus_tag="OQM87_000240"
     CDS             110604..111662
                     /locus_tag="OQM87_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506517.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNCQAK
                     YQNEINQLSALGVNFDDI"
BASE COUNT        27993 a        29871 c        27463 g        26386 t
ORIGIN      
        1 attttgttat tatttgatga ataatatcag tgcgtcataa ttcaagttaa taaccttaag
       61 ggataccagt tatatttaaa ctaaattaaa gtaatgaata attttcttat aatataaggt
      121 aaattaacaa aatggcttcg catttaacaa taaccgaata gaaaacaacc atttcgacat
      181 caacaatcgc ttacattcgc ttatatattg accacaactg atacatcaga ttatgtgatg
      241 actcgtgctt agatcaattt ttgcaatcat tagcaaaaag attaataaac catctatatc
      301 aatttatcca acctattctg ccgttcaaga aatcgccgaa cagttatttt taacaaattt
      361 ttctcttccc attgactttc ccggacacct tgtctgacct aaggtgcgcg aaagccactt
      421 tttccttcct gagttatcca caaagttatg cacttgcaag agggtcattt tcacgctatc
      481 ttgcagtgaa tcccaaacat accccctata tatagtgttc taagcagctt cccttactac
      541 aggtagtctg catgaaacta ttgcggaaag aattccaaaa acaggtacga catacatgaa
      601 tcagaatctg ctggtgacaa agcgcgacgg tagcacagag cgcatcaatc tcgacaaaat
      661 ccatcgcgtt ctggattggg cggcagaagg actgcataac gtttcgattt cccaggtcga
      721 gctgcgctcc cacattcagt tttatgacgg tatcaagacc tctgacatcc acgaaactat
      781 tatcaaggct gccgcagacc tgatctcccg tgatgcgccg gattatcagt atctcgccgc
      841 gcgcctggcg atcttccacc tgcgtaaaaa agcctacggc cagtttgagc cgcctgcgct
      901 gtacgaccac gtggtgaaaa tggtcgagat gggcaaatac gataatcatc tgctggaaga
      961 ctacacggaa gaagagttca agcagatgga cacgtttatc gatcacgacc gtgatatgac
     1021 cttctcttat gctgccgtta agcagctgga aggcaaatat ctggtacaga accgcgtgac
     1081 cggcgaaatc tacgaaagcg cccagttcct ttatattctt gttgccgcgt gcctgttctc
     1141 gaactacccg cgtgaaacgc gcctgcaata cgtgaagcgt ttttacgacg cggtttccac
     1201 atttaaaatt tcgctgccga cgccaatcat gtccggcgtg cgtaccccga ctcgtcagtt
     1261 cagctcctgc gtactgatcg agtgcggtga cagcctggat tccatcaacg ccacctccag
     1321 cgcgattgtg aaatatgttt cccagcgtgc cgggatcggc atcaacgccg gacgtattcg
     1381 tgcgctgggc agcccgattc gcggtggcga agcgttccat accggctgta tcccgttcta
     1441 caaacatttc cagacagcgg tgaaatcctg ctctcagggc ggtgtgcgcg gcggtgcggc
     1501 aacgctgttc tacccgatgt ggcatctgga agtggaaagc ctgctggtgt tgaaaaacaa
     1561 ccgtggggtt gaaggcaacc gcgtgcgtca tatggactac ggggtacaaa tcaacaaact
     1621 gatgtatacc cgcctgctga aaggtgaaga tatcaccctg ttcagcccgt ccgacgtacc
     1681 ggggctgtac gacgcgttct tcgccgatca ggaagagttt gaacgcctgt ataccaaata
     1741 tgagaaagac gacagcatcc gcaagcagcg tgtgaaagcc gttgagctgt tctcgctgat
     1801 gatgcaggaa cgtgcgtcta ccggtcgtat ctatattcag aacgttgacc actgtaatac
     1861 ccatagtccg tttgatccga ccatcgcgcc agttcgccag tctaacctgt gcctggaaat
     1921 tgccctgccg accaaaccgc tgaacgacgt caacgacgag aacggtgaaa tcgcgctgtg
     1981 tacgctgtct gctttcaacc tgggcgcaat taatagcctg gatgaactgg aagagctggc
     2041 gattctggcg gttcgtgcac ttgacgcgct actggattat caggattacc cgatcccggc
     2101 cgccaaacgt ggagcgatgg ggcgtcgtac gctgggtatt ggtgtgatta acttcgctta
     2161 ctatctggcg aagcacggta aacgctactc cgacggcagc gccaacaacc tgacgcataa
     2221 aaccttcgaa gcgattcagt attacctgct gaaagcgtct aatgagctgg ctaaagagca
     2281 aggcgcgtgc ccgtggttta acgaaaccac ttacgcgaaa ggtatcctgc cgatcgatac
     2341 ctataagaaa gatctggatg ccatcgctaa tgagccgctg cattacgact gggaagcatt
     2401 gcgtgaatca atcaaaacgc acggcctgcg taactccacg ctttctgccc tgatgccgtc
     2461 cgagacctct tcgcagatct ctaacgccac taacggtatt gaaccgccgc gcggttacgt
     2521 cagcatcaaa gcgtcgaaag acggtatttt gcgccaggtg gtgccggact acgagcacct
     2581 gcacgacgcc tatgagctgc tgtgggaaat gccgggtaac gatggctacc tgcaactggt
     2641 aggtatcatg cagaaattta tcgatcagtc gatctctgct aacaccaact acgatccgtc
     2701 acgtttcccg tcaggaaaag tgccgatgca gcagttgctg aaagacctgc tcaccgccta
     2761 caaattcggg gtcaaaacgc tgtattatca gaacacccgt gacggcgctg aagacgcgca
     2821 ggacgatctg gtaccgtcaa tccaggacga tggctgcgaa agcggcgcat gtaagatctg
     2881 atattgagat gccggatgcg gcgtaaacgc cttatccggc ctacggctcg gtttgtaggc
     2941 ctgataagac gcgccagcgt cgcatcaggc tccgggtgcc ggatgcagcg tgaacgcctt
     3001 atccggccta cggctcggat ttgtaggcct gataagacgc gccagcgtcg catcaggcac
     3061 aggatgcggc gtaaaatgcc ttatccggca ttaaactccc aacaggacac actcatggca
     3121 tataccacct tttcacagac gaaaaatgat cagctcaaag aaccgatgtt ctttggtcag
     3181 ccggtcaacg tggctcgcta cgatcagcaa aaatatgaca tcttcgaaaa gctgatcgaa
     3241 aagcagctct ctttcttctg gcgtccggaa gaagttgacg tctcccgcga ccgtatagat
     3301 taccaggcgc tgccggagca cgaaaaacac atctttatca gcaacctgaa atatcagacg
     3361 ctgctggatt ccattcaggg tcgtagcccg aacgtggcgc tattgccgct tatttctatt
     3421 ccggaactgg aaacctgggt cgaaacctgg gcgttctcag aaacgattca ttcccgttcc
     3481 tatactcata tcattcgtaa tatcgttaac gatccgtctg ttgtgtttga cgatatcgtc
     3541 accaacgagc agatccagaa acgtgcggaa gggatctcca gctattacga tgaactgatc
     3601 gaaatgacca gctactggca ccttctgggc gaaggcaccc acaccgttaa cggtaaaacc
     3661 gtaaccgtta gcctgcgcga gctgaagaaa aaactgtatc tctgcctgat gagcgttaac
     3721 gcgctggaag cgatccgctt ctacgtcagc tttgcttgtt cttttgcctt tgcagaacgc
     3781 gaactgatgg aaggtaacgc caagattatt cgcctgattg cccgcgatga agccctgcac
     3841 ctgaccggta ctcagcatat gctgaatctg ctgcgcagcg gcgcggatga tccggagatg
     3901 gcggaaattg ccgaagaatg taagcaggag tgctatgacc tgtttgtcca ggcggcacag
     3961 caagagaaag actgggcgga ttatctgttc cgtgacggtt cgatgattgg tctgaataaa
     4021 gatattctct gccagtacgt tgaatacatc accaatatcc gcatgcaggc agtcggtttg
     4081 gatctgccgt tccagacgcg ctccaacccg atcccgtgga tcaacacctg gctggtgtct
     4141 gataacgtgc aggttgctcc gcaggaagtg gaagtcagtt cttatctggt cgggcagatt
     4201 gacgcggaag tggacaccga cgatttgagt aacttccagc tctgatggcc cgcgttaccc
     4261 tgcgcatcac tggcacacaa ctgctgtgcc aggatgaaca cccttccctt ctggcggcgc
     4321 tggaatccca caatgtggcg gttgagtacc agtgccgcga aggttactgc ggctcctgtc
     4381 gcacccgact ggtcgcaggt caggtggact ggattgccga accgttagcc tttattcagc
     4441 cgggggaaat tttgccctgt tgttgccggg caaaaggcga tattgaaatc gagttgtgat
     4501 cgggtactag cgccagatac gacgcttacg cgtattatct ggcctacaac gattacatgg
     4561 cgtagtaata cgctttcacc tgctcccagt ccgctttagg gatcggctcc agatattttt
     4621 ccagctggcg gaagtcatga ttaatcgctt tatcgcgacg caagcggcga cgacttttct
     4681 ccagatcaag aaaaccagct tctgccttac cttctgtttt cacatagata tggcgaacat
     4741 agcaacagcc atgctgacga ttaacgctat gcattttctt aaacgccagt gccactgctt
     4801 tcaacatggc ttgccgtact tcgtcagaat aaggcgatac tgcatgccgg gcataccagt
     4861 cagcaatgct gatgaacccc gccatatctt cagtcaccag caacgcgcgc cattcaccct
     4921 caattttcac cgcttcacca aaaacgatct tcggcacgat gacaccggcc cgttccagtt
     4981 ctttaattac cgcaacctca cggacaatcg ttggtcggcc gaacggataa cgtactgaat
     5041 gaaacagatg atgcgtcatg cgctttacat acagcttttt gccgttgcgc tcgacgcatt
     5101 gcaccctgct cataccatta cggcgatagt taggctcttc aacccagtcg ccctctgttg
     5161 cccaccagtg attaaattcg tcgtactttg ctgaaactgc cataccctat cgcctgtcat
     5221 ttttattaac gacaatgact ataggtggtt acctgagcaa aatcttaatg aaacgtgtcg
     5281 tattccgcta caggtgtgac accgcttttg cccttcattt gcagcgcata gattccacca
     5341 gccactacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag tcggatacag
     5401 cgatgaattt tattcgacag ggattaggca tcgccttaca accagagtta acgctgaaaa
     5461 gcattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga cagatttcgt
     5521 tgctgactaa agaaaagccg gtagaaggca gtccactgtt tttactacaa atgtgcatgg
     5581 aacaattagt ggcgattgga aaaatttgat atgagacagg atggcgcatg aacgccatcc
     5641 tgcagtaaca ttactcttta ttaagaaatt ttactgcctt atcagggaaa tcagtaaaca
     5701 gcccatttac acccgctttg ttatacagag catcatataa ctgattcaca tcaggagtgt
     5761 attcaggcag tttatctgac cgcacggtat aaggatgcac taccagttta ttctgctgag
     5821 catcttgcac catgccagtg agtttgatat tacccggctg cgatgtctcc tcaatcaaca
     5881 tatggtaatc cggaccaata ccatctgcat attccgccac ctgtttcatg gcacccggct
     5941 taaacatcca gtcgtagttg taattaaccc agcttccatc cggctgtttc tgctgcgttt
     6001 cattccagtc ggtataggca atcagctgta ccagattgag ctccatgccc attttgggtt
     6061 ccagctcatt cttaatacgc ttcagctcat cagcatcaaa acattgcaaa taaactttat
     6121 cgtctttacc ggtgtaacca tatttcttca gcacttccag cgtttttgcc gcaatatcct
     6181 tcccttcctg atgatggaac cacggcgctt tgatttctgg atagataccg atatttttcc
     6241 cggtagagtg atttaacccc tgaacaaatt caatctcttc ttcaaaggtg tgcacccgga
     6301 agtcggactt acccattggg aaacgccccg gataagtctg tactttttta ccgttttcaa
     6361 tatcgaaacc ttcggtaaat ttcagcgact taatttcatc cagcgtgaaa tctatcgcgt
     6421 agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc ggcaacatca gtaacacgat
     6481 cgagataatg gtcatgcaga acaaccagat ggtcgtcttt ggtcatcacc aaatcctgct
     6541 ccagataatc cgctccctgc gcatacgcca tcgcttttgc tggcagcgta tgctccggca
     6601 aatatccact ggcaccgcga tgggcgatga ctattttttc gttgctgtcc gccgccattg
     6661 cactgcttcc catgactata gtgctcatca tgatcgccat gctaaggttt ttcagcttca
     6721 atttcatgcc attagcctcc gttgcgtttt tgcagtaatt gttcatggcg acgtttttcg
     6781 ccaatcatca caacaatcaa caagataacc gccagaatgc tgccgccaat cattaccata
     6841 aagccgccat cccagccgaa gaagtccacg gtgtagccaa caatcgcgct cgccgccacc
     6901 gaaccgccca ggtaaccaaa cagcccggta aagcccgctg ccgtacctgc cgcttttttc
     6961 ggtgccagtt ccagcgcatg cagaccgatc agcatcacag gaccgtagat caggaagccg
     7021 ataacaatca tacaaatcat atcgacggtt gggttacctg ccgggttcat ccagtaaacg
     7081 atagtcgcga tggtcaccag tgtcataaag aagacgccgg ttgccccacg gttaccacgg
     7141 aagactttgt ccgacatcca gccgcacagc agggtgcccg gaatacccgc atactcatag
     7201 aagaagtagg cccaggagga tttatccagc gcgaaatgct taacctcttt cagataagtc
     7261 ggtgaccagt cgaggatgcc gtaacgcagc agataaacga acacgttggc gatggcgatg
     7321 taccacagca gtttgttcgg cagcacgtac tgcatgaaga tttgcttcgc ggtcagctcc
     7381 tgttccgctt tttcgttata gtcgtccgga taatcatttt tgtactcttc gatcggcggc
     7441 aagccacagg attgcggggt atcgcgcatc atcgcaaagg cgaataatgc caccagaatg
     7501 gcgcagaaag caggcatata gagcgccgca tgccagtcat tgaaccaggc catccccagc
     7561 aggaacagca gcggcggaat accaccaccg acgttgtgcg cacagttcca cactgacaca
     7621 atgccgccac gttctttctg cgaccaccag tgcaccatag tacgaccaca cggcggccac
     7681 cccatcccct ggaaccaacc gcagaggaac aacagtacaa acatcaccgc aatgctcgac
     7741 gtcgcccatg gcacaaagcc cataaacaac atcactgccg ccgccagaat caaacctgcg
     7801 ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac ccatgatgaa tttcgaaaat
     7861 ccataagcaa tcgagatccc cgaaagggca aaacctaaat caccgcgtga gaatccctgc
     7921 tcaaccagat aaggcatagc aagcgcaaag ttcttacgaa ccaaatagta agccgcatag
     7981 ccaaagaata tccccaggaa aatttgccag cgcaatcgac gataagtcgg atcgatctcc
     8041 gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa tactcaacat tgatagcctc
     8101 cgtggcccgt ggtcttattt atgattaaca gcctgattca gtgagagaac ctgccgtttc
     8161 ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa tgtgaattgc cgcacacatt
     8221 attaaataag atttacaaaa tgttcaaaat gacgcatgaa atcacgtttc actttcgaat
     8281 tatgatcgaa tatgcgcgaa atcaaacaat tcatgttttt actatggcta aatggtaaaa
     8341 aacgaacttc agagggataa caatgaaaac tcgcgactcg caatcaagtg acgtgattat
     8401 cattggcggc ggcgcaacgg gagccgggat tgcccgcgac tgtgccctgc gcgggctgcg
     8461 cgtgattttg gttgagcgcc acgacatcgc aaccggtgcc accgggcgta accacggcct
     8521 gctgcacagc ggtgcgcgct atgcagtaac cgatgcggaa tcggcccgcg aatgcattag
     8581 tgaaaaccag atcctgaaac gcattgcaca tcactgcgtt gaaccaacca acggcctgtt
     8641 tatcaccctg ccggaagatg acctctcatt ccaggccact tttattcgcg cctgcgaaga
     8701 agcagggatc agcgcagaag ctatagaccc gcagcaagcg cgcattatcg aacctgccgt
     8761 taacccggca ctgattggcg cggtgaaagt tccggatggc accgttgatc catttcgtct
     8821 gaccgcagca aacatgctgg atgccaaaga acacggtgcc gttatcctta ccgctcatga
     8881 agtcacgggg ctgattcgtg aaggcgcgac ggtgtgcggt gttcgtgtac gtaaccatct
     8941 caccggcgaa actcaggccc ttcatgcacc tgtcgtggtt aatgccgctg ggatctgggg
     9001 gcaacacatt gccgaatatg ccgatctgcg cattcgcatg ttcccggcga aaggatcgct
     9061 gctgatcatg gatcaccgca ttaaccagca tgtgatcaac cgctgccgta aaccttccga
     9121 cgccgatatt ctggtgcctg gcgataccat ttcgctgatt ggtaccacct ctttacgtat
     9181 tgattacaac gagattgacg ataatcgagt gacggcagaa gaggttgata ttctgctgcg
     9241 tgaaggggaa aaactggccc ccgtgatggc gaaaacgcgc attttgcggg cctattctgg
     9301 cgtgcgcccg ctggttgcca gcgatgacga cccgagcgga cgtaacgtca gccgtggcat
     9361 cgtgttgctc gaccatgctg aacgcgatgg tctggacgga tttatcacca tcaccggtgg
     9421 caaactgatg acctatcggc tgatggctga atgggctacc gacgcggtat gccgcaaact
     9481 gggcaacacg cgcccctgta cgactgccga tctggcactg cctggttcac aagaacccgc
     9541 tgaagttacc ttgcgtaaag tcatctccct gcctgccccg ctgcgcggtt ctgcggttta
     9601 tcgtcatggc gatcgcacgc ctgcctggct gagcgaaggc cgtctacacc gtagcctggt
     9661 atgtgagtgc gaagcggtaa ctgcgggtga agtgcagtac gcggtagaaa atttaaacgt
     9721 taatagcctg ctggatttac gccgtcgtac ccgtgtgggg atgggcacct gccagggcga
     9781 actctgcgcc tgccgcgctg ccggactgct gcaacggttt aacgtcacga cgtccgcgca
     9841 atctatcgag caactttcca ccttccttaa cgaacgctgg aaaggcgtgc aacccatcgc
     9901 ctggggagac gcactgcgcg aaagcgaatt tacccgctgg gtttatcagg gactgtgtgg
     9961 tctggagaag gagcagaaag atgcgctttg atactgtcat tatgggcggc ggcctcgccg
    10021 gattactctg tggcctgcaa ctgcaaaaac acggcctgcg ctgtgccatt gtcacgcgtg
    10081 gtcaaagcgc gttgcatttc tcatccggtt cgctggattt gctgagccat ctgccagatg
    10141 gtcaacccgt ggcagacatt cacagtggac tggaatcttt gcgtcagcag gcacctgcac
    10201 atccttactc tcttctcggg ccacaacgcg tgctcgatct cgcttgccag gcgcaggcat
    10261 taatcgctga aagcggtgcg caattgcagg gcagcgtaga actggctcac cagcggatta
    10321 cgccgctcgg cactttgcgt gcaacctggt taagttcgcc agaagttccc gtctggccgc
    10381 tacccgcgaa gaagatatgt gtagtgggga taagcgggct gatggatttc caggcgcatc
    10441 ttgcggcggc ttcgttgcgc gaactggatc tttcggttga aaccgcagaa atagagctgc
    10501 cggaactgga tgtcctgcgc aataacgcca ccgaatttcg cgcggtgaat atcgcccgtt
    10561 tccttgataa tgaagaaaac tggccgctgt tacttgatgc gcttattcct gtcgccaata
    10621 cctgcgaaat gatcctgatg cccgcctgct tcggcctagc cgatgacaaa ctgtggcgtt
    10681 ggttgaatga aaaactacct tgttcactga tgcttttgcc aacgctgccg ccttccgtgc
    10741 tgggcattcg tctgcaaaac cagttacagc gccagtttgt gcgccagggt ggagtgtgga
    10801 tgccgggcga tgaagtgaaa aaagtgacct gtaaaaatgg cgtagtgaat gaaatctgga
    10861 cccgcaatca cgccgatatt ccgctacgtc cacgtttcgc ggttctcgcc agcggcagtt
    10921 tctttagtgg cggactggta gcggaacgta acggcattcg cgagccgatt ctcggccttg
    10981 atgtgctaca aaccgccacg cggggtgaat ggtataaggg cgattttttt gcgccgcaac
    11041 cgtggcagca gttcggtgta accactgatg agacgctacg cccgtcgcag gcagggcaaa
    11101 ccattgagaa cctgtttgcc atcggttcgg tgctgggcgg atttgatccc atcgcccagg
    11161 gatgcggcgg cggtgtttgt gccgtcagtg ctttacatgc cgctcaacag attgcccaac
    11221 gcgcaggagg ccaacaatga acgacaccag cttcgaaaac tgcattaagt gcaccgtctg
    11281 caccaccgcc tgcccggtga gccgggtgaa tcccggttat ccagggccaa aacaagccgg
    11341 gccggatggc gagcgtctgc gtttgaaaga tggcgcactg tatgacgagg cgctgaaata
    11401 ttgcatcaac tgcaaacgtt gtgaagtcgc ctgcccgtcc gatgtgaaga ttggcgatat
    11461 tatccagcgc gcgcgggcga aatatgacac cacgcgcccg tcgctgcgta attttgtgtt
    11521 gagtcatacc gacctgatgg gtagcgtttc cacgccgttc gcaccaatcg tcaacaccgc
    11581 tacctcgctg aaaccggtgc ggcagctgct tgatgcggcg ttaaaaatcg atcatcgtcg
    11641 cacgctaccg aaatactcct tcggcacgtt ccgtcgctgg tatcgcagca tagcggctca
    11701 gcaagcacaa tataaagacc aggtcgcttt ctttcacggc tgcttcgtta actacaacca
    11761 tccgcagtta ggtaaagatt taattaaagt gctcaacgca atgggtaccg gtgtacaact
    11821 gctcagcaaa gaaaaatgct gcggcgtacc gctaatcgcc aacggcttta ccgataaagc
    11881 acgcaaacag gcaattacga atgtagagtc gatccgcgaa gctgtgggag taaaaggcat
    11941 tccggtgatt gccacctcct caacctgtac atttgccctg cgcgacgaat acccggaagt
    12001 gctgaatgtc gacaacaaag gcttgcgcga tcatatcgaa ctggcaaccc gctggctgtg
    12061 gcgcaagctg gacgaaggca aaacgttacc gctgaaaccg ctgccgctga aagtggttta
    12121 tcacactccg tgccatatgg aaaaaatggg ctggacgctc tacaccctgg agctgttgcg
    12181 taacatcccg gggcttgagt taacggtgct ggattcccag tgctgcggta ttgcgggtac
    12241 ttacggtttt aaaaaagaga actaccccac ctcacaagcc atcggcgcac cactgttccg
    12301 ccagatagaa gaaagcggcg cagatctggt ggtcaccgac tgcgaaacct gtaaatggca
    12361 gattgagatg tccacaagtc ttcgctgcga acatccgatt acgctactgg cccaggcgct
    12421 ggcttaaact cccttctgat gcccggtaag catgtggtta ccgggcattt ttgcgtacac
    12481 gattccgtgc ccaatgtatg cgttgcaacg cagtgaaaat tcctctgaaa acgtctcgca
    12541 aaggctgaaa ctggcagatg tcaaaggcct gggataaccg taatgtcgcg tcatcataaa
    12601 tatcaggtga cggacaacca tgaccgaatc aacaacctcc tccccgcatg atgcggtatt
    12661 taaaaccttt atattcacac ccgaaaccgc acgggatttt ctcgaaatac atttaccaga
    12721 accactgcgc aagctttgca acctgcaaac cttacgcctg gaacccacta gttttattga
    12781 aaaaagttta cgcgcttact actcggatgt tttgtggtcc gtggaaacca gcgacggtga
    12841 cggctatatc tactgcgtga ttgaacatca aagctctgca gaaaagaata tggcttttcg
    12901 gccaatgcgc tatgccactg ccgccatgca gcgtcaccta gataaaggct atgacagagt
    12961 tccgctggtg gtgccattgc tgttttatca tggcgaaaca tcgccctacc cgtactcact
    13021 taactggctg gatgagtctg acgatccgca acttgcccgg cagttgtaca ccgaagcttt
    13081 tccgttggtg gatatcacca tcgtacctga cgatgagatc atgcaacatc ggcgtatagc
    13141 tctgctggaa ctgattcaaa agcatattcg cgaccacgat ttaatcggca tggtcgacag
    13201 gatcaccacg cttttggtta gaggcttcac taatgacagc cagctacaaa cactgtttaa
    13261 ttatctgctg caatgcggcg atacctcccg tttcacccgt tttattcagg agattgccga
    13321 acgttcacca ctacaaaagg agagattaat gactattgct gaacggctac ggcaggaagg
    13381 gcatcaaatt ggctggcagg aaggtatgca tgaacaagcc attaaaattg ccttgcgcat
    13441 gctggagcag ggctttgaac gtgagattgt gctggcggca acccaactca ctgatgctga
    13501 tattccgaac tgttattaaa aggagaggga gagattgatg actattgctg aacggctacg
    13561 gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc agcaaggtaa
    13621 attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt tgcgcatgct
    13681 ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg aagccgatct
    13741 ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg cctttgatat
    13801 caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc cagtcgttga
    13861 tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt cgctccccac
    13921 gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc tttacccgca
    13981 gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg cccggcgtta
    14041 tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc atcaatccct
    14101 tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac ctgcactaac
    14161 agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca gcgcgcagcc
    14221 cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa attttgccgg
    14281 agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac ggataaagtc
    14341 atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc gtttaaccaa
    14401 cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag
    14461 cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag
    14521 ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc
    14581 cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaatcact
    14641 tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg
    14701 aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg
    14761 ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc
    14821 cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc
    14881 ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt gtccaggctt
    14941 tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcggctgt
    15001 ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat
    15061 tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac ggcaaaacca
    15121 caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta
    15181 cggctgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc
    15241 caggcttcat gcagcacaag gccttcacct tttgctgtcg cagcggcgag tgcgcttaaa
    15301 tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt
    15361 tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat
    15421 ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc
    15481 ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt tatctcctgc
    15541 ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatgcc
    15601 gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca gccacagcgt
    15661 tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc tcaatactga
    15721 ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt tgaataccgc
    15781 tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg ttgccaattt
    15841 tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa ttcgggttgg
    15901 tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga ttcaacccca
    15961 gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg cttaaagcca
    16021 ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta cccgcctggc
    16081 cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc agacctgcta
    16141 gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc ccaaagctgc
    16201 gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct agtcacttat
    16261 tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact ttaccgatac
    16321 acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc actttcacat
    16381 atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac ccctgtgcga
    16441 caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct gtgcaaaatg
    16501 gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg cgtggagtct
    16561 ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg agaacagctg
    16621 cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa gacatatgca
    16681 gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt ttctgccaac
    16741 cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc tgaagatctg
    16801 gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg tcttctttaa
    16861 caatgatgac agccatcact ggctgtcagg ttgaaaattt agtggttaac gaattcccca
    16921 tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata atcaggttta
    16981 aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg ggtgaaaata
    17041 accatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata aatctcatta
    17101 ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa ccaggtcgcc
    17161 gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat gtcagcgcta
    17221 aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc tgttttatct
    17281 gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa aacgacaacc
    17341 gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa accattgcta
    17401 ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa aatgatgaaa
    17461 tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc acctgtagac
    17521 aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa ttagcagttg
    17581 gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc caaatgcaaa
    17641 gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag tttcttaagg
    17701 ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac acaacactct
    17761 ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat gtcagaattt
    17821 ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa agaggttctc
    17881 gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc cttttgccag
    17941 ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat gcatatcacg
    18001 ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct gacctgggtt
    18061 tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga tgtcgaccgc
    18121 gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg cactaaagcc
    18181 atcattccgg tgcattatgc cggtgcgcca gcagatattg acaccattcg cgccattggc
    18241 gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac gtattacaaa
    18301 gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat taaaaatatt
    18361 acctgtgctg aaggtggcct gattgtaact gataatgaaa accttgcccg ccagctacgg
    18421 atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg gggccgtgca
    18481 ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat taacgccgcg
    18541 attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg cgaaattgcc
    18601 cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct tcccgcctgg
    18661 ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg ttgtggtatc
    18721 agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg gttacatttc
    18781 cgcgccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc gttaccgaat
    18841 accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat gactaccgcc
    18901 gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc catgtttgaa
    18961 atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca ggaaagctta
    19021 ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga gtatgagatc
    19081 ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga agcctcacaa
    19141 gcggagaaca gccatattgt gtctattttg cttaaccgca attacgggca acattcagcg
    19201 attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga tgccgatctc
    19261 cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg ttacgacgtg
    19321 gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc ttcgaagatg
    19381 attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg ttgtatgctg
    19441 cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg cagcaccttt
    19501 atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt acatcatgcc
    19561 gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt gatgtacgac
    19621 ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg cagcattatt
    19681 gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac cttcggacca
    19741 caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt tattggcgct
    19801 cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac cgatgtccgc
    19861 gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga aaatgaataa
    19921 tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa gctctgctgg
    19981 ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt gaaaaagcct
    20041 tttatggttc ggtggctcat ctggcggcgg aaagagacat tccggtttat gcgccggata
    20101 acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag gtgattttct
    20161 ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc gcaggtgcat
    20221 ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg aactgggtgc
    20281 tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa cgtgccgatg
    20341 ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc gctattacgc
    20401 tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta cccgccatta
    20461 aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt tttggtcgca
    20521 gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg cacaacatgg
    20581 tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat cagaaattca
    20641 ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg agcgtgattt
    20701 ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc accggacagg
    20761 cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc ctggtgcaag
    20821 gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg gtactcatcc
    20881 tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc gaagatcatt
    20941 atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg aatcatccac
    21001 attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag tatcacgtca
    21061 aaaaatgtga tttcgtcttg ccgctggtgg cgatagccac gccgattgaa tatacccgca
    21121 acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc cgctactgcg
    21181 ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg atgtgtagcg
    21241 ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat aaaccacgct
    21301 ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat ggcgaaaaag
    21361 agggtttaca gttcaccctc ttccgcccgt ttaactggat gggaccacga ctggataacc
    21421 ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc aatctggtag
    21481 aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt actgatatcc
    21541 gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc tgcgacggcg
    21601 aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg ggcgagatgc
    21661 tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt gcgggctttc
    21721 gcgttgtcga aagtagcagc tactacggca aaggatatca ggacgtagag catcgtaaac
    21781 cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat atgcaggaaa
    21841 ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat aaaccatcat
    21901 gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg aaggcgtgcc
    21961 gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt tcagcgtcgg
    22021 cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt tgtggaagat
    22081 gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag gtacggcctg
    22141 gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg ctaaacatca
    22201 cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg gtaactggga
    22261 tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag agattatcgg
    22321 tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg tgatcgaagc
    22381 aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatgc cgttccgtcc
    22441 actgctcgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac ccacctggga
    22501 tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac tcaaccgcat
    22561 cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag gctgcgctta
    22621 tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga cattttgccc
    22681 tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg gaaatattgc
    22741 cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat gaaatcggta
    22801 cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc gatcagcacg
    22861 cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga aatgctggca
    22921 tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa acccattgcc
    22981 ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt tggtgtgcgg
    23041 gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt tactctgcgc
    23101 ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc attgtttatt
    23161 gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg gctggtggcg
    23221 ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa aagcgcagga
    23281 tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt tctcgccctt
    23341 gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg gaaagatctc
    23401 tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc ctggggactg
    23461 gcgatagcgc agcgggagcc tgacttgtgg cactattttt tctgggttga gcatattcaa
    23521 cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt gccggtcatc
    23581 attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac aggctggaaa
    23641 aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc gctgctgttt
    23701 ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc acctctggca
    23761 atgctgttgg cgcattacgc tttgctggca gcaaaaaata atcctctggc gctgcggatt
    23821 aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt tgtgatctcc
    23881 ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa agtcttttgt
    23941 gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac aaacgtcgaa
    24001 aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt aggattttca
    24061 attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat gacacaagaa
    24121 tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc aggtctggca
    24181 tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt gaaatacggc
    24241 cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc aaactggctt
    24301 aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg cgatgaagat
    24361 atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg tctggtgctg
    24421 attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg cttagcgttg
    24481 ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa cggcgcaaac
    24541 atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg gtgctctggc
    24601 tgctggtctt gcagaacgta ccggtaggca ttgcctaccc gatgttaagt ctgaattttg
    24661 tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg cgtcactggt
    24721 gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg taatgggcct
    24781 gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc tgggttttgc
    24841 ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc tgttagcttt
    24901 tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt ccgttttttg
    24961 ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc tcagtatgag
    25021 ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga ctttttcgct
    25081 gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt tcctgcccac
    25141 gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc actcatcggg
    25201 gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat taatacaata
    25261 aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt cactcttcac
    25321 ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat gtctgttatc
    25381 ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc tgtttgctaa
    25441 gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc aatggaatgg
    25501 cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca ctcttttagc
    25561 gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt cagccagcgc
    25621 accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc aacggtttgc
    25681 tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc aacggaaacg
    25741 ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc ttccggctga
    25801 ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt cagcttgcca
    25861 ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac cagtgaaacc
    25921 agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca cacttcatta
    25981 ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag gacgtcggct
    26041 tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa gcaacgaatc
    26101 ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc aagtaacacc
    26161 gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt tggcaccagt
    26221 gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg caccgtcatc
    26281 cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg aaacagcggt
    26341 aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac accttgcgca
    26401 ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt agaacccgag
    26461 gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac gccttcaacc
    26521 agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg caccagcgca
    26581 aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag ctgagggttc
    26641 acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag cgtttgcggc
    26701 gtattccacg cccgcaacat cacgccgctg ccctcaacca caccctgcgc cgcaaagccc
    26761 gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc gtcattaaga
    26821 cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca gtcagagaag
    26881 atcataacaa ctgctccagt acatcagcgt ccactaacgg cagtgggctc cccggccagc
    26941 gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg gtgtctggcg
    27001 ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca atggaagaac
    27061 tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt actttttcca
    27121 gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca gccacaaagg
    27181 caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt tcacgggcaa
    27241 acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg cggtggcgat
    27301 aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag gcgcgatttg
    27361 cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc accatgccgt
    27421 cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc ggcatatcgg
    27481 caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca cggtagttgg
    27541 ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca aaggccacgg
    27601 aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag gcaagcagca
    27661 cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag atctcccccc
    27721 acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt tttaaccgcc
    27781 tgtcgcgcag aagcaccccc gcgtccatgg ggatctgcca gcggtatacc tgcgcgctac
    27841 gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt tgaaagcgtt
    27901 gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc ccgccagctc
    27961 ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc agcgcagcgc
    28021 catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt ccagatccgc
    28081 cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt cgtactgacg
    28141 gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca tgtaggaagc
    28201 gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga tggcattatc
    28261 tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga tggagtagcc
    28321 agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga agtccagcac
    28381 gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac gcactttctg
    28441 gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca cgccgatatt
    28501 gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca gaggacggaa
    28561 ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg tggatttttc
    28621 ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg cgtaaggcat
    28681 tgcttcatca ggataaatca tattgtgtcc tttagtcaaa aacgcaagat ctgcgccaga
    28741 cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg aggaatgaca
    28801 tggcagtcgg cagccagttc cgccgctagg gcgcggaatt tgctgtcacg ttcaccacat
    28861 aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg ctggacggcg
    28921 agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct gcgcagcgcc
    28981 accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg ataccagtcg
    29041 gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg atcggaacgc
    29101 tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc gataataacc
    29161 ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc gccaagcgag
    29221 taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg cagtaagtcg
    29281 gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc tgggagatca
    29341 acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc gtggcaatcg
    29401 ccggaaaaac cgtgcaaaaa caccagccag ggtaaacctg gttttccgtg ttttgcctgc
    29461 gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt tgcgcaccat
    29521 cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag gcgtcggcaa
    29581 atgccgtttc aagttcctgc cagttttgcg gacgatgata tttcagctcg aacatcgcgg
    29641 cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc tcgctttgcg
    29701 gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt aataccagcg
    29761 gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagtgcg gagagatcgc
    29821 ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg gtcgaaagca
    29881 gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc ggaagttgcg
    29941 aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc ccctgttcag
    30001 gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca cggcgggcaa
    30061 taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag ggctggcgtt
    30121 tttctgccgg atgcagctcc agccagtcgg caatattgcc aattaagcga cgtccgcgat
    30181 ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct tctggttcac
    30241 agcttgcctg ccattgcagg agccgtttcc ccgtcaggct gcttcccagt tgcaccacaa
    30301 tttgtgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga tcggcacacg
    30361 gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca agagtttgcg
    30421 cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc accacgccgc
    30481 gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga ggcgcttcac
    30541 gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag ctaagcccgg
    30601 tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg actccccccg
    30661 catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg gggatatcct
    30721 gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt cccggctggc
    30781 gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg gttaagagaa
    30841 tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt gccaccgccg
    30901 tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc cccagcgcca
    30961 gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa ttctccgccg
    31021 ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt ctgacgccgt
    31081 gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg cttactgaca
    31141 tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa tagattattg
    31201 atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt acgcagccct
    31261 gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg gacaatgccc
    31321 gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag ggaaacgcag
    31381 aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc tcgggtgaac
    31441 ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc aactgctgcc
    31501 gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga agtccagata
    31561 cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa aacatccagc
    31621 gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat gttctcgcgc
    31681 tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt atcatcagga
    31741 tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg ccgccgccat
    31801 aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat gtaaaaatgg
    31861 tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt gaccggactt
    31921 gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc ttcggcgatg
    31981 gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca gtgttgttct
    32041 cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt ggcgataaat
    32101 tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt caaccgcagc
    32161 gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt gccctgcgac
    32221 gggtcaaaag cattcagccc ccaaatgcgt aagtcggcgt gttccgggtg ctggcgaaga
    32281 aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac ggcagcttct
    32341 tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact ggccagccag
    32401 ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg aatgccgggt
    32461 gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt agtaagtgat
    32521 tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac aaaaaaaatg
    32581 cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag taccagcccg
    32641 acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc atcagcacga
    32701 taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac ccgttttttc
    32761 acatcatcca gcgctttttc tgcacgcgct ttcagctcaa catatttctg atcggcgggc
    32821 tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag gtcgtcatcg
    32881 atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa aacctgtcac
    32941 taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat cccaatgtgt
    33001 gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact ctggtagaag
    33061 ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg atgcgtacat
    33121 gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa cgcttcactg
    33181 acaatcaccc gacctataac gacaggctca agatcatcat cacttttcag aatcctcgca
    33241 tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc cgtcaaatca
    33301 tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc gcaacgcagt
    33361 tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc ttgccattca
    33421 atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt tttgaaacgt
    33481 gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac ccgcacgcgc
    33541 aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact ttggttgttt
    33601 gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc tggaaaactg
    33661 gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc cggcttgctg
    33721 tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc gcaaggtatc
    33781 cgtgaatttg tggaaaccgc gcagcggatt agcggctcat ggaccgatta tccgctggaa
    33841 attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt aaccgcttat
    33901 ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga taaaccgggt
    33961 gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact gtttcaggaa
    34021 ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg cgcagattac
    34081 ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg cccctgcgat
    34141 gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac gctggatata
    34201 accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc tgcgacacta
    34261 gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg ctatgatgac
    34321 aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac tgaactggcg
    34381 aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg gtgaaacttg
    34441 ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg agcgattttt
    34501 gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc ttttcatctt
    34561 gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg tcagcaatta
    34621 ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta ctgtgaccga
    34681 ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa gtattgagtt
    34741 actgcataat attgcgttag gttatgatga atttggaaaa aaagctgaag atattcttta
    34801 ccatattgtt agaaacccaa caaatgagac cctatcgatt atccgactta ttaaaaatgc
    34861 ctgtttaaaa ttatataatt tggcacatac cgcaaccaac tcccacctta aaccaactgg
    34921 tccggataat tcagatgttc tcctgttcaa aaagctattc tccccttcga aattaatgac
    34981 aattatcggt aatgaaattc ctcttatatc tgaaaaacag tcgctttcaa aggtgctttt
    35041 aaatgatgag aataatgaac tgagtgatgg tacaaacttc tgggataaaa atcgtcaatt
    35101 aaccacagat gaaatagctt gctatcttca gaagatcgcc gctaatgcaa aaaatactca
    35161 agtcaattat cctactggtc tctacgtccc ctactccacc agaactcacc tggaagacgc
    35221 tctcaatgaa aatattaaga gcgatccatc atggccgaat gaagtccagt tattccccat
    35281 aaatactggc ggacactgga tattagtttc gctacagaaa atagtaaata aaaaaaataa
    35341 taaactacaa ataaaatgcg tcatattcaa ctcattgcgt gcactaggcc atgataaaga
    35401 aaattcactt aagcgtgtca ttaatagttt taattctgaa ctcatgggaa aaatgtcgaa
    35461 taataatata aaagttcatt taaatgaacc agagataata tttttacatg ccgatcttca
    35521 gcaatactta agccaaagtt gcggtgcgtt tgtgtgcatg gcagcccagg aagtgattga
    35581 acaaagggaa agcaattctg acagcgcccc ctatacgtta ttaaaaaact atgctgacag
    35641 atttaaaaaa tattcagcag aagagcagta cgaaattgat tttcaacatc gactggtaaa
    35701 cagaaattgt tatttagata aatatggcga tgcacgaatc aatgcttctt atacacaatt
    35761 agaaataaaa cactcacaac ccaaaaatag agcatccggc aaaagagtga gttaacatca
    35821 ttgttaatca attcgaccag tcagtaaata tgctgactgg tcgaacataa tttgctaaaa
    35881 agtcatcact gactgatgtc agtcccacca tctgccagtt caatctacgg gtatttccag
    35941 cgtatcgcgg cacgcgattg ctggtctgtt gatgtagtgg attgtagttg ctgacttttc
    36001 ttaccctcag atttctgccc cacttgtcaa ttcaatctgc caataaataa cataattata
    36061 tttttattcc tcacagaaat tcctttttaa aaatagcgaa cccactgtaa aaaacacttt
    36121 ttgtatacct ctaccaagcg taataaaata aacgctcata attcattata aataataatg
    36181 aaataacgcc ttttattgat aatttacata tgaaaaaaat tttccttttg ttatattatt
    36241 tctgttttca ctccctctga ttatttttac caaccctggg tcactgcatt atcaccaacg
    36301 ccccgacacg taagtcctga gcaattagaa aaaacagttc gttatcttac acaaactgtg
    36361 catccacgta atgccggcaa tattgataat ctgaataggt ccgcagagta tataaaagag
    36421 ttctttgtca gtagcggtgc cagagttacc gcgcagggcg tccccgattg gctattacga
    36481 ctctgcgcct ggaagccaga attaccctta cccggcaatg tcctggcttt atcccgatca
    36541 gggagacttt attgctgtgg tcggcagaat gcaggatatc aacgccgttc gtcaggtaaa
    36601 agcggcattg ttgtcatccc gggatttatc tgtttattct atgaatgccc cagggtttat
    36661 tcccggtatt gatttttctg atcaccttaa ttattggcaa cacgatattc ccgccgtaat
    36721 gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg ataccgcaga
    36781 cagattgaat tatcagaaaa tggctcaggt ggtagatggt gttataacct tgttatacaa
    36841 cagtaaataa taaaaatttc tcgaggcggg tccagaaaca gcgacatgta cattttattt
    36901 tccctcgtct gttttaagat gcgaaacata aaattcaatg gaggaaaaat aaatgggaat
    36961 gattggctat tttgcagaaa ttgactcaga aaagattaac caactgcttg agtctactga
    37021 aaaacctttg atggataata tacatgacac gctttcagga ttacgccgac tggatattga
    37081 taaacgctgg gattttttac actttgggct gacaggcacc tctgcttttg atcccgcgaa
    37141 gaatgatccg ctgagtcgtg ccgtgcttga tgaacacagc ctcgaagatg gcatagatgg
    37201 cttcctggga ttgacctgga atcaggaatt agccgcgaca atcgaccgac tggaatcact
    37261 ggatcgaagt gagctgcgta aacagttttc cattaagcga ctcaatgaaa tggagattta
    37321 tccgggagtg accttttcag aagagttaga ggggcaactc tttgcctcga tcatgcttga
    37381 tatggaaaag ctgattagcg cgtatcgacg tatgctgcga caagggaatc atgcgctgac
    37441 ggttattgtt gggtgatggt gtcagagcgc caaaaaaaac cgccgaacat gtcggcggct
    37501 ttctgactta caaactaaca gtttacatca gcggcattgc taaccgcaca atactaatca
    37561 gcggttgtgg ccatacaccc agcaccagta ccaacagtgc agaaatcagc accacgatac
    37621 cgcccgcgct gtactgccag tttgacggtg catcgcgacc cggttgttcc ggtgcgtgca
    37681 ggtacaggct caccgccacg cgcaggtagt agtagaggcc gattgccgaa ccgacaacca
    37741 cggcacccac cagccaccac aagtgtgcct ggacaccgac cgccagcacg tagaacttac
    37801 cgataaagcc cagcgtcatc gggataccgg ccagagacag catcatcacc gtcatcactg
    37861 ccgcgaggat cggacgatgc cagaacagac cgcggtaaga gaacagggaa tcagcatccg
    37921 ggccacgata cgggctggac atcaggctga ccacgccgaa cgcgccgagg ctgctgaaca
    37981 gataaccggc caggtaaacc cctaccgctt ccatcgacat ctcgccggtt tgcagcgcaa
    38041 tcagcgccac cagcagatag ccgaggtgag agatagatga gtaaccgagc agacgtttga
    38101 tgttggtctg gctcaacgcc atcaggttac cgaagatgat ggaggcaaag gcgataatcg
    38161 ccagtactac gcgaatcgct tcgctgtcac ccaccggtgc gtagaggaac agacgcatca
    38221 ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa agtggaaacc ggcgcaggcg
    38281 cgccctggta tacgtctggc gtccacaggt ggaacggcac cagagagagt ttgaagccga
    38341 ggccaacaat catcatgccg aaacctgcca gcaacagcgg ctcgttgagc ataccgtcgc
    38401 cgaggttttt acccagcgcg acaaacgaca ggtcgccaga ctgcgcatac accagcgcca
    38461 taccgaacag caggaaagaa gacgctgcgg cagaaaggat ggtgtatttg atactggctt
    38521 ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc aaacagcggc aaagagatca
    38581 gttcaatacc gaggaacaga gacgccagat ggtttgcatt cgccagcagg atcccgccca
    38641 gcgcggcaat taacaccaac aggtagaact catccttgtt gtcgttatag ccttcaagcc
    38701 acgggtagtc gaaagtacag gtggcgaggc tcgccaacaa taccagcccg gtgtaaagca
    38761 tggcgaaacc atcaacgcgc atcagcggcg taacgtccat agcgcccgcc tggccaacaa
    38821 accagagcga aaccagcgcc gcgttaagcc caataaccga gagcgtagcg ttgaggaaat
    38881 gattgcgtcg ccacgcaatg gagagcatca caaccaccac cgtcaagccg acgatcagca
    38941 acggtagcag tgcgatcagg ttttgtggag ttattgtcat ggcgatttac ggccttgtag
    39001 tagtaacgga attaacaaac cactgctgga tattgccaat cgcggagtgc gaggtatcca
    39061 gaatcggctg cggatagaag cccagcagta ccagcagcac caccagcaac aggatcataa
    39121 acagctcacg cagcgacatc cctggcagtt cctggctggc aatctggctt ttcgctttac
    39181 cgaagtaagc gcgatgcaac atcgccagcg aataaacaga tgcaaagacc agcccaaagg
    39241 tagagataac ggtaatcacc gggacaacct ggaagctgcc gaacagaatc ataaattcgc
    39301 cgacgaagtt accggtgcca ggcatcccaa gcgttgccac cgcaaagaac agcgacagtg
    39361 ctggcagcca tttcatcttg ctccacagac cgcccatcat gcgcatgtcg cgggtatgga
    39421 tacgttcata aagctgaccg cagagaataa acagacccgc cgccgacaag ccgtgcgcaa
    39481 tcatctggat taccgcgccc tggtaggcca actggctgcc ggtgtagatt gcaatcagca
    39541 cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg tttgatatcg gtctgagcga
    39601 aggccatcca cgcaccgtag aagatgccga taacacccag ccacatagcg attggcgcga
    39661 actctgccga cgcgttcggg aacagcggca gggagaaacg caacaaaccg taagcggcgg
    39721 ttttcagcaa gatccccgcg aggtccacgg aaccggcggt cggagcctgg gagtgcgcat
    39781 ccggcagcca gccatgcagc ggaaccaccg gcattttgac tgcgaaggcg atgaagaagc
    39841 ccagcatcaa cagatattcc acaccactgg acattggcgt attcagcagc tcttcatagt
    39901 tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac cagcgccagg atggcgatca
    39961 acatcaccag accactcgcc tgggtgtaaa tgaagaactt ggttgccacc gtgatacgcg
    40021 ttttaccgtc agaggcttta tgcccccaca gtgcgatcag gaagtacatc ggcaccagca
    40081 tcatttccca gaagaagaag aacaggaaca tgtcgatggc aaggaacacg ccgataacgc
    40141 cgcccaggat ccacatcagg ttgaggtgga agaagccctg atatttttcg atctctttcc
    40201 acgaacatag taccgccagc acaccgagca gaccggtcag cacgaccatc agcagcgaca
    40261 gaccgtcaat ggcgagatga atagagatac caaaacgcgg gatccacggc atgtcgaatt
    40321 cagactgcca ctgcggaatt ccggcggatt gcgtcagtga ataaccgccc tgcaaccaca
    40381 gttgcagcga cagcgccagc gtcaatccca tggtgatcag cgcgatccag cgtggcacct
    40441 tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc aataaaggga attaatatta
    40501 gccagggtag taacatggcg atctttattc cttgtaaaag tcccgtcagg accggatttt
    40561 caacgaattc tcacgacaga aaattctctt cggactgggg gtagtgtcgg atgcgccgcg
    40621 aaggcagctt atccgaccta cgttcttata tgcccagggg caatcccaca atccttaact
    40681 caacgcagta ccatcaacag tgccagcacc acgaccgcac cgatgctcat ggatgccaca
    40741 taccagcgca gataaccgtt ctcgcttaac agcagacctt tacctgcaaa gcgggaaaga
    40801 accgccggga tgttcatcat tgagttcagc ggatcgcgtt tcagcaacca ggcaataccc
    40861 aggaacggct tgacgaacac tttgtcgtac agccagtcaa agccccaggc gttgtaccac
    40921 caggtactca gcaggcggcc cggcgcactg ttggcgatgg aggtcaccag agtacgttta
    40981 cccagccaca gccaggctgc cagcagaatg ccgaccaccg cgaccacgcc agaggtaatt
    41041 tccagggtca acatgctgcc gtgcgccagt tccgtcgttt gcggaagcac gccctgcagc
    41101 ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca gcagcacaat cagcggcagg
    41161 ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt cttttccgtg gaagacgatg
    41221 aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac cgaccagacc tgccaccatc
    41281 agattgatat gaccattcgc catcgcaccc gcgaggatct catccttact gaagaagccc
    41341 gcagtgacca gcggcagtgc cgacagtgct gcgccgccca ccaggaagca gagataaacc
    41401 agcggaatag atttacgcag accgcccatc ttgaagatgt tctgttcgtg atggcaggcc
    41461 agaatgacgg aaccggatgc caggaacagc agcgctttaa agaacgcgtg agtcatcaag
    41521 tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga ggaacatgta gccaatctgg
    41581 ctcatggtag agtaagcgag aacacgtttg atgtcggtct gtaccagcgc ggcaaaaccg
    41641 gccagcagca gcgtaaccgc cccgacaata cccaccagat gcagaacttc cggcgtcatc
    41701 aggaacaggc cgtgggtacg ggcgatcagg tagacacccg cggttaccat cgttgcggcg
    41761 tggatcagcg cggagacagg cgtcgggccc gccatcgcgt cagcaagcca tgtctgcaac
    41821 ggcaactgcg cagatttacc gaccgcaccg cccagcagca tcagcgtcgc ccacatcagc
    41881 atgttattgc cgtcagcaaa gtgcgctggt gccagttcca ccatttcgcg gaagttcagg
    41941 gtgcccagtt cgttgtaaag aatgaacagt gcgaaagcga ggaaaacgtc acccacacgg
    42001 gtcacgacga acgctttcat tgctgctgcg ccattcttcg gatcggtgta atagaaaccg
    42061 atcagcagat aggagcacag gcccacgcct tcccagccga ggtacatcag cagcaggtta
    42121 tcggcgagaa ccagaactac catgctggcg atgaacaggt tggtgtaggc gaagaagcga
    42181 gagtagccct cttcaccgcg catgtaccag gaggcgtaca tgtgaataag gaaacccaca
    42241 ccagtgacca ccgagagcat ggtcagcgac aggccgtcca gcaccaggtt aaaaccgatg
    42301 ttaaagtcgc ctaccgacat ccacgtccac agcggctggc tgtatgcctg ctcgccgtta
    42361 gcgaagaaat caacgccgat aaaggcggtt acctgcgccg ccaggcccac agagccaacg
    42421 ccgacgatcg ccgaaacgtt ttcagaccag cgcccacggg agaacgccag caggacgaag
    42481 ccaatcaatg gcaaaataat ggttaaggca agcatgttca tccgcgcatc tcacttactg
    42541 aatcgatgtt caggttctgg cgacgacggt gaagttgcag cagcagcgca aggccgatac
    42601 tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat cacctgaccg tcggtctgac
    42661 cccagtagct tccggcgacc acaaaggcca gcgcggaggc gttaatcatg atttccagac
    42721 caatcagcat aaacagcaga ttgcgacgga taaccagacc ggttaagcca agaacgaata
    42781 agattgccgc gaggatcagt ccatgttgta aggggatcat gcgtgctcct ccgtttttct
    42841 tttcgcgctg tcgtctttac gattgctcag cacttcaccc gcacgctctt cacgaccgac
    42901 gtggaaggcc acaaccagac ccgcgagcag cagcatagaa gccagttcca ccgccagtac
    42961 gtaaggcccg aacagcgtaa tacccactgc tttagcactg attggcgtac cgtcgatacc
    43021 ctgatcgtta acaccgagga tggcgtaaac aatcaccacc agcatgatgg ccgacaaaat
    43081 tgccgggcca atccacacct gcggtttcag ccactggcgt tcctgttcga tttctgaacc
    43141 gcccaggttg agcatcatca ccacgaacac gaacagcacc ataatggcac ccgcgtagac
    43201 gataatttcc agcgcaccgg cgaagtaagc gcctagtgag aagaacaccc cggaaatcgc
    43261 cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga ttggtatggg tgatcactcg
    43321 caaggtcgca agtatggcta tcaggccaca gatataaaaa gcgaactcca ttgcccctct
    43381 ccttacggta acaggctctt gacgtcgata ggcttggctt cgttctctgc ttcgccctta
    43441 tctttgccgt cgattgccat acctgccatc cggtagaagt tatattccgg gtatttgccc
    43501 ggaccggaga tcagcagatc ctctttctcg taaaccagat cctggcgctt gtattccccc
    43561 atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac aggcttcttc gcacagacca
    43621 cagaaaatgc agcgtgagaa gttaatgcgg aaaaattccg gataccagcg accgtctttg
    43681 gtttctgctt tttgcagcga gatacagccg accgggcagg ctaccgcaca gaggttacag
    43741 gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa cgatacgacc acgataacgg
    43801 ggcggcagat agaccggctc ttccgggtac attcgcgttt cgcgtttggc gaacgcgtgc
    43861 aggccgatca tccagatact acgaacctgg gtgccgaaac ctactaacaa ttcttttaag
    43921 gtcatggtct tattgcccct tattgcgcct gccagagaat gacagccgcc gttaccagca
    43981 agttgatcag cgtcagcggc aggcagattt tccagccgaa ggacattacc tggtcataac
    44041 gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat cataaagaac gcggttttca
    44101 gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca gccaccgaag aacagcgtca
    44161 ccatcaatgc agagatggtc acaatcccga tgtactcacc cacgaagaac agaccgaact
    44221 tcataccgga atattcaatg tggtaaccat ccgccagttc ctgctcggct tccggctggt
    44281 caaacgggtg acggtgacac accgccacgc ccgcgatggc aaaggtaata aaaccaaaga
    44341 attgcgggat gacgttccac acatgcgcct ggctgttgac gatgtcggtc atgttgaatg
    44401 aaccggcctg cgccaccacg cccatcaggg aaagcccgag gaacacttcg tagctcaggg
    44461 tctgcgcaga agcacgcatc gcacccagca gcgagtattt gttgttactt gaccagcccg
    44521 caaacagcac cgcgtaaacc gccagacctg ccatcatcag gaagaacaaa atcccgatgt
    44581 tcaggtcggc aaccacccaa cccggactga ctggcacaat tgcaaaggcc agcagcagcg
    44641 aggtaaaggc aatcatcggt gccagggtaa agatgacgcg atccgagaat ttcgggatcc
    44701 agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag ctggagcgaa ccgccccagc
    44761 caacacggtt aggtccataa cggttctgga acagacccag caggcgacgt tcgccaaagc
    44821 tcatgaatgc cccacaggtg acaaccacca gcaggatccc caccgctttg aggatggtca
    44881 gcaggatctc aatcagttcc ggtgatatcc aactcattgt tgtgcctcct tgagatcctc
    44941 aagatgcgcg ccagccagca ccggagcaat gccggacata cccatcggca agcccacctg
    45001 ccctgccgtc agtccttcgg cgatttcaac cggcagcgtg accgtattgc catcgtaact
    45061 aaaggagacg cgtgtacctg cgttcacacc caacttcgcg gcatccgctg ggttgagttt
    45121 gatgtacggc tgcggcatac ggctctggaa gaccggagca cgctgtgaca attcatcgct
    45181 gccaaacagg tggtagtacg gcgcgatacg ccatttcccg tcctgcggct ggaagcgtgc
    45241 cggtacgctg gtgaagtaat ccagaccgtt ttcgctggtt tcaaacagac gcacgcccgg
    45301 atcgccaaag cgcagtttgc cgcccacttc gtcctggaat ttgttccacg cctgcgggga
    45361 gttccagccc ggcgcccagg caaacggcac ttgcgaacgg tgcgcggtcg gctggttgtt
    45421 accttccatc gagaaggtga acatggtgtc aatatcctgc ggctgacgcg gctcatgaac
    45481 gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg tgcggttcac gggccagttt
    45541 ctgcccacga atacggaatg tcgcatccgg cgcagcatct ttgatacccg ccagttccgg
    45601 gattttcgcc acaacagcgt caattacatg gtcgagctgc gtccagtcca cttcacggct
    45661 cagcaaggtg ctgtgcagcg agtgcagcca gcgccagctt tccagcatga cagttttgct
    45721 gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg gcgcgacctt cattattaat
    45781 taccgtaccg tcgctttcag caaagctggc agcggaaagc accagatggg cgttttccat
    45841 gatcgctgtg cgttgatgat caaccaccat caccagcggc gctttagcca gcgcagcatt
    45901 cacgcgggta gcagaagcgt aacgatgcag atcgttttcc aacaccacca ccgcgtcggc
    45961 gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgaa ccgccaccca taatgcccag
    46021 ccccatgctg ttgacggaac gggcaatcat ggtgatacca acgtcagcgc cgcgaccttt
    46081 cagggctttc gcgacgttag ccgcagcctg aatcacctct atgctaccgg cgttcgtccc
    46141 ggagataatc aacggtttct tcgcacctgc cagtgcctgc acgatgacgt cgattttgtt
    46201 ttgcagctca ggttcgatac cgtcaaccgc tggtgcggag ttatccagcg catgggcgat
    46261 ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg taagtccacg ccgcgatatc
    46321 atccagacga gtgtcatcaa cgttggtaac aaacagcgga tgcttcgcac gttgaccgat
    46381 gttgaggatt gccgcaatct gccagtcagc cactttctgt gctgcagcca tttcgcgcgc
    46441 tttacctttc accgcctgac gcactgccag tgcgacgcgc gcgccggtct gggtaacgtc
    46501 ttcgcccagc accagtaccg catcgtaaga ttcgatttcg cgcagagccg gagtataaat
    46561 gccgccttca cgcagcactt tcagcgccag ttgcagacgt tcctgctcac cgtgagcgat
    46621 accggtgtag aagttttctt cgcccaccag ttcacgcagc gcaaagttgc tttccacgct
    46681 ggcacgcgga gaaccaatac cgatcacttt cttcgactga cgcagaatat ctgccgcgcc
    46741 ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg ccacgacgct gtactggctg
    46801 acgcggacga tccttcaggt tgacgtaacc gtaaccgaaa cgaccacggt cgcagaggaa
    46861 gtagtggttt accgtaccgt tgtaacggtt ttcgatacga cgcagttcgc cgtaacgttc
    46921 accggggctg atgttacagc cgatggaaca ttgctggcag atgctcggcg caaactgcat
    46981 atcccattta cggttgtaac gctcggagtg cgttttgtcg gtaaatacgc cggtcgggca
    47041 aatttcgacc aggttaccgg aaaattcgct ttccagcgtg ccgtcttccg ggcgaccgaa
    47101 gtagacgttg tcgtgcgcgc cataaacgcc cagatctgta ccgtcagcgt aatctttgta
    47161 gtaacgcaca cagcggtagc aggcgatgca gcggttcatt tcgtgagaga tgaatggccc
    47221 caaatcctga ttacggtggg tacgtttggt gaaacggtag cgacggaagc tgtgtccggt
    47281 catcacggtc atatcctgaa gatggcagtt accgccttct tcacataccg gacagtcgtg
    47341 cgggtggttg gtcattaacc actcgaccac actttcacgg aactgtttcg cttcttcgtc
    47401 gtcaatggaa ataaaggtgc catcggaagc cggtgtcata caggacatca ccaggcgacc
    47461 acgcgtgtct tccgcgtttt ggtattgctt caccgcacac tggcggcaag caccgacgct
    47521 tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg cccagagaca gacaagcttc
    47581 cagcaggttg tccgctccgt tgacctcgta ttctttgccg tctacatgaa ttgtagccat
    47641 tagcatgctt ccagttttct cggtcaggga ccgagcgtta atcgaaattc ggttaccagc
    47701 gctctttcag caggttcggc tgaatcccat taatcaaatg ggtattgctg aacggctgtt
    47761 tgattcccgc ctcaaattct tcgcggaaat atttgatggc gctctgtaac ggctccactg
    47821 caccaggtgc gtgggcacag aaagttttac ccgggcctaa gaatcgacac agttgctcaa
    47881 gtgtttcgat atcgcccggc tggccttcgc cacgctccag cgcacgcaga attttcacgc
    47941 tccacggcag accgtcgcgg cacggcgtac accagccgca ggactcacgg gcgaaaaact
    48001 cttccaggtt acgcaccagc gacaccatgt tgatctcatg gtcaaccgcc atcgccagcg
    48061 ccgtacccag acggctgccc gctttaccga tactttcgaa ttccatcggc agatcaaggt
    48121 gcgcttcggt caggaagtca gtccccgcgc cgcctggctg ccaggcttta aatttcagac
    48181 catcacgcat accaccggcg taatcttcga ggatctcgcg tgcggtggtg ccgaacggca
    48241 gttcccacag tcccggattt ttcacccgac cggagaagcc catcagcttg gtgccagcat
    48301 ctttactttt cgagatgttc tgataccact ccacgccgtt agcgaggatc gccggaacgt
    48361 tacacagggt ttcgacgttg ttgacacagg tcggtttgcc ccatacgccg gaggttgccg
    48421 ggaagggggg tttcgagcgt gggttagcac gacgcccttc cagggagttg attaacgctg
    48481 tttcttcccc gcagatgtag cgccctgccc cggtatggac gaacagttcg aagtcgaaac
    48541 ctgttcccat aatgtttttg ccaagcaagc ccgcttcagt ggcttcggca atagcgcggc
    48601 gcagatttac tgccgcttcg atatattcgc cacgcaggaa gatgtagcca cggtaagctt
    48661 tcagcgcaaa cgcggagatg agcatacctt ccaccagcag gtgcggcagt tgctccatca
    48721 acaggcggtc tttataggtg cccggctcca tttcatcggc gttacacagc aggtaacgga
    48781 tgttcatgga ttcgtctttc ggcatcaggc tccacttcag gccagtcgaa aagcccgcgc
    48841 cgccgcgccc tttcagacca gcgtctttta cctgattaac gatttcgtcc ggagacagcc
    48901 cggtcagcgc cttacgcgcc ccttcgtaac cgtttttgct gcggtattcg tccagccaca
    48961 ctggctgttt gtcatcgcgc agacgccagg tcagcggatg cgtttcggga gtacggataa
    49021 tgtttttcat ttataccgct ccagcagttc agggatcgct tccggggtca gatgcgcgtg
    49081 agtgtcctca tcgatcatca tgtttggccc tttatcacag ttccccaggc agcaagttgg
    49141 cagcagcgta aagcggccat caaatgtcgt ttgccctggt ttgatgttca gctttttctc
    49201 gagcgccgcc tgaataccct gatacccgtt gatatgacag accacgctgt cgcaataacg
    49261 gatcacatgg cgaccaaccg gctggcggaa gatctgactg tagaacgttg ccacaccttc
    49321 gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg tggatcgcac catccggcac
    49381 ccagccacgc tgcttctgaa cgattttcag cgcttcaatg gacgccgcac gcgggtcttc
    49441 gtagtggtgc atctcgtgct caatcgcttc acgctctgcc gcactcagct caaaagcctc
    49501 ggtttgtggt tgttgattct cgtgcataat tagcggtcca catctgacat aacaaaatcg
    49561 atactgccca gataaacaat caggtcagac accaggctgc cgcggatcgc cgccggaatt
    49621 tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc gataactcat ggtgctaccg
    49681 tcgctggtca ggtagtaact gttgatcccc ttggtcgcct caatcatctg gaaagattca
    49741 ttggcaggca tcaccggccc ccacgacact tgcaggaagt gggtgatcag ggtttcgata
    49801 tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg ggtgatccgc tttgaacggg
    49861 ccttccggca tgttgttgag gcactgctca agaatgcgca gactctggcg cagctcttcc
    49921 actttcagca ttacgcgggt gtagcagtca gaaacaccac caccaaccgg gatttcaaag
    49981 tcgaagtttt catagccaga ataaggacgc gccttacgca cgtcgaagtc gatcccggta
    50041 gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct ctttcgcgcc ataggcggca
    50101 acgccctggg tacgaccttt cagaatggta ttttgcagcg ccgctttctc gtaagacgcc
    50161 agacgtttcg gcatccagtc gaggaactca cgcagcagac gatcccagcc gcgcggcagg
    50221 tcgtgcgcta cgccgccaat acggaaccac gccgggtgca tacggaaacc cgtgatcgct
    50281 tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga agaacacggg cgtcattgcg
    50341 ccgacgtcct gaataaaggt cgagatgtac agcaggtggc tgttgatacg gaacagttcg
    50401 gagagcataa cgcgaatgac gttaacgcga tccggcacgg tgatcccggc cagtttctct
    50461 accgccagca cgtaaggcat ttcgttaacg cagccgccga ggtattcaat acggtcggta
    50521 tacggaatgt agctgtgcca ggactggcgc tcgcccattt tctccgcacc acggtggtgg
    50581 tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt cgagttgtaa aacgatacgg
    50641 aaagccccgt gcgccgacgg gtggttcgga ccgaggttga ggaacatgaa gtcctcgttt
    50701 tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg tcagggcttc catctccaga
    50761 tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg tagcgcgcgc cgggtaatct
    50821 ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga tgcggcgcag gttcgggtga
    50881 ccgtcgaaag taatgccaaa cagatcccag gtttcacgct cataccagtt agcgttcggg
    50941 aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg ccagcgccac cttcagcatg
    51001 atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa cggaaaaatc cgcggcaggt
    51061 aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt gtaagtcaaa cagcatgacg
    51121 taaggtttcg gcagtttctt taagaaatcg ccaacttcca gtaattgttc acgcttgatc
    51181 cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa aggcatccgg cccaaaacgg
    51241 ttgcgcagtt cgccaattac cggatcatca agatgatcgc gggtctgcca ggcgggttct
    51301 tgcgcggtta agtcggtcat attgttcacc attgcaaatg gtccgtggtg actgtcggcg
    51361 cagtaagctt cgcgaaatag atttaggaat aagcgaagaa aaatcccttt gccgacaggc
    51421 gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg caatgcgttc accgcgcttg
    51481 cgttcgcgct ctgattgcat attggcgcga taaacgccct gatcgccaac cacccaggag
    51541 agcggacgac gttctttgcc gatagattcc tgcaacagca tcagtgcctg catgtacgct
    51601 tcaggacgcg gcgggcagcc cgggatatac acatcaaccg ggatgaattt atcgacgccc
    51661 tgcacaacgg aataaatatc gtacatacca ccagagttgg cacaggcccc cattgagata
    51721 acccattttg gttccagcat ctggtcatac agacgctgaa taaccggtgc cattttggta
    51781 aagcaggttc ctgcaaccac catcaggtca gcctgacgcg gcgaagcacg caatacttct
    51841 gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc aacgtaacag
    51901 caggaaagac cgaagttata cggccaaatt gagtttttac gaccccagtt aaccatgtca
    51961 ttgagcttgc ccataaacac gtttttgtta acttcttgct ccagagggtc ggttacgatc
    52021 tcctgctttt gcagggggta acggtcgttc tcaccgttgg gatctatgcg ggtgagcgta
    52081 taatccatct taatgcctcg cggttagcgt tgacgattag cgatactgtt cgtttccggg
    52141 ttcatacact cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat acgcaccaga
    52201 taaaccagac ctgccagtaa cacaaaaata aaaattgcag cttccacaaa gcctacccag
    52261 ccgctttcgc ggatagaggt tgaccatgcg aacagataca gcgcttcaac gtcgaagata
    52321 acgaagaaca tggccaccag ataaaacttg gcggacaggc gtaagcgggc ggagccgacc
    52381 gaatcgatac cggattcaaa cggcacgttt ttcgacctcg cgcgtgcgcg accgcctaaa
    52441 aaccaaccgc ctaccagcat caggcaacac aggccaatgg caacgataag aaagatagcg
    52501 aatgcccagt gatgagcgat gacttcagtg gatgttgaca tactcattgc ttactcatca
    52561 aaagtagcgc cagattcact gctcttcacg gcagatggac gccacatcga ttcatgggga
    52621 ggaataaaaa aaaccttaca atcactgtag aaattctttt atacagctaa ttgatgtggt
    52681 cttttactcc tttctataac cttttgtcaa ctttaacaaa agtttcttca cattagttta
    52741 cataatatca acaccattag catttaatgc cctttcaccc cagatccttg acgactccag
    52801 gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat acgggtctat
    52861 tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt gatctgtgac
    52921 acgcttttgt tattccataa caaaaacgca gcaacaaatt tacgtatttt ttaacatcat
    52981 tgtagcaggt gatttttttc aggcgattat ttgtgcgttc gggacgtgaa tctctggtga
    53041 ttgaaaaata aacagttttg tacgttctgc actatgcgag gaaggatttt tactaaaaaa
    53101 aagccgctgg ggtttaaaac acccccagcg gctcgttttt tacactattg tctcaggaat
    53161 tatctatcgt ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc atccccttcc
    53221 ggagcagaca ttgggctgta ttgccacgga ttatggtagc tctccatcgc ctgatagatc
    53281 acctgggcca gctcattatt actggacggg tcatagcaca gcaaatattc ggtatcaggt
    53341 agcggcggta agccatctac tccactcagc acacgcaggt ccgggctcat catctcaacc
    53401 ggccttgccg tcacgccaag accggctttc actgccgcac gaacggccgg aagcgtcgag
    53461 gcgacataag ccagtcgcca tggaatatct gctttattaa gcgtcgccag caccatatcg
    53521 cgaaacggac ttggatcatc cagcaataca agagggatcg gctcgccttt ttgcaatacg
    53581 tattccgctg cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc cttaaacgct
    53641 gaaggacgat gggtggttac catcaaatcc acttcctggg attccagcat ttctgccata
    53701 taggcattac gtttaacgcg gacatccagc gctaatttcg gataaaccga actcacgcga
    53761 tttaacagga aaggtaagat cgtatcggca gattcatctg aagcaccgat agttaacacg
    53821 ccctgaagat tactgaacat taatgatgag caggcctcat cattaaaacg caggattttc
    53881 ctggcgtaac caagaagttg aatgccatgt tcagttaaca gtttgttgcg accgtgacga
    53941 gcgaacagtt ctttcccaac gagttgttcc agacgctgca tttgctgact tacggcggac
    54001 tgagtacgac acacagcggc agctgcggca gcaaaagtgt tcagatcggc aacagcaaca
    54061 aatgttctca gcagatcgag gtcgaggtta attatcggac gatttgcact tatcatatat
    54121 tatcacttac tggcggctca tactgagctg gttaatgctg tgcacacaca aacaagcaat
    54181 tccatttgta atgtgcctcc ctggcagttt catccggaac ccggacgaaa gtaaaaatgc
    54241 atatgagttg cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg aagtagatca
    54301 cagaatatat cttcagggat cgcatatcta ttaagttact cactcttttc tatttatgac
    54361 atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc cactctcgtg cagcatcgct
    54421 ggtcatacgc gaacacgtac aaacagcaat ggtgaagcgg catcaagagc agggatccgt
    54481 catttatgcg agcattttac cccaaaacct tttatttata agggtcattg cgaattatct
    54541 gatgcaaagt tatgttatgt tatgttatgt tatgttatgt tatgttatgt tatgttatgt
    54601 tatgttaggc aaagtaatct tctattatta ataagcacat caaaaccttt ttgaatatta
    54661 aataataatt aattagcatc atcctcattc attaattccg cttaacaata gtttcacacc
    54721 ttatccccat tgcgacacct cggaaatcat cacgctgtta aactctccgt aatgcggaaa
    54781 tatcatcatc gcgtttcatt agtgaattct taaatgaggc attttcacac aattatctta
    54841 cagataaaaa aaccagactt gcaattaaga attagaacaa ttcactatat aacattacat
    54901 gtaaagcata tacacctcat tattttgtca ttattaagtt attaacagca caatcgagcc
    54961 ttcccctctg gcaaaatctt attctgcaga ccttcaaaac accgtcctgg gggagtacat
    55021 tgttctaagc tgacttccac ggcagggagt ggcgataaca gcaaaaaagg tcaagattca
    55081 tgtcccccat tgaaaaatcc agcaaattag agaatgtctg ttatgacatc cgtggtccgg
    55141 tgctgaaaga agcaaaacgc ctggaagaag aaggtaacaa ggtactgaaa ctgaacatcg
    55201 gcaacccagc cccattcggt tttgacgcgc cagatgaaat cctcgttgac gtgatacgca
    55261 acctgcctac cgctcaaggg tattgcgatt ccaaagggct ttactccgcg cgtaaagcca
    55321 tcatgcagca ctaccaggct cgtggcatgc gtgatgttac cgtggaagat atttacatcg
    55381 gcaatggtgt atcggagctt atcgttcagg caatgcaggc attgctgaac agcggggacg
    55441 aaatgttggt tcctgcacca gattacccac tctggaccgc ggcggtttcg ctttccagcg
    55501 gtaaagcggt gcattatctt tgcgatgaat cctctgactg gttcccggac ctcgatgata
    55561 ttcgcgctaa aattacgcct cgtacgcgtg ggattgttat tatcaaccca aataacccaa
    55621 ccggcgcggt atattccaaa gagcttttaa tggagattgt ggagattgca cgtcagcata
    55681 atctcattat cttcgccgat gaaatttatg acaaaattct ctacgacgat gctgagcatc
    55741 actcaattgc gccgctggca cctgacctgc tgaccattac ctttaacgga ctgtcgaaaa
    55801 cgtaccgcgt tgcaggcttc cgtcaggggt ggatggtgtt gaacgggccg aaaaaacacg
    55861 ccaaaggcta catcgaaggt ctggaaatgc tggcttcaat gcgcctgtgt gctaacgttc
    55921 ctgcgcaaca cgccattcag accgcgctgg gtggttatca gagcatcagt gaatttatta
    55981 cccctggcgg tcgtctttat gagcagcgta accgcgcgtg ggaactgatc aacgatattc
    56041 cgggcgtttc ctgcgtgaaa cctcgtggtg cgctgtatat gttcccgaaa atcgacgcca
    56101 aacgctttaa cattcatgac gatcagaaaa tggtgctgga tttcctgttg caggaaaaag
    56161 ttctgttggt gcaagggacg gcattcaact ggccgtggcc ggatcacttc cgcattgtca
    56221 cgctaccgcg tgtcgatgat atcgagctgt ctttgagcaa gttcgcgcgt ttcctttctg
    56281 gttatcatca gctgtaatct taatttcact gccggagatt gcatccggca gtgttatccc
    56341 gccacaatga cctgatgatg tcatcatacg taaggtcact atgaaacaga gccatttttt
    56401 tgcccatctc tcccgcctga aactcattaa ccgctggccg ctaatgcgca acgtgcggac
    56461 ggaaaatgtg tccgaacaca gtttgcaggt agcgatggtc gcccatgcgc tggcagctat
    56521 caaaaatcgg aaatttggcg gtaatgtcaa cgccgaacgt atcgctttac tggcgatgta
    56581 ccacgatgcc tcagaagtgc tcaccggcga tctccctact ccggtgaaat acttcaatgc
    56641 gcaaatcgct caggagtaca aggctattga aaaaatcgct cagcagaaac tggtcgatat
    56701 ggttccggaa gaactgcggg atatctttgc gccgttaatt gacgagcatg catatagcga
    56761 tgaagaaaaa tcgctggtga aacaggcaga tgcactgtgt gcatatctga aatgtctgga
    56821 agaactcgcg gccggaaata atgaattctt gctggcaaaa acgcgactgg aagcgacgct
    56881 tgaagcgcgt cgcagccagg agatggacta cttcatggaa gtatttgttc ccagcttcca
    56941 tctttcgctc gatgagatta gccaggattc accgctgtaa gcagccggag tctgcgtcgc
    57001 atcaggcaat aagcgccgga tgcgacatca ggctcttgtc aaaacggaaa cagcatcggg
    57061 atcatcacca cacaaaccgc catcacgata atggtgaacg gtacccccaa cttcacaaag
    57121 tcactgaagc tgtaatttcc cggacctaaa accagggtgt taacaggtga agacaccggt
    57181 gtcataaagg cggcggatgc tgccatcgcc acgaccatcg caaatggata aggcgacact
    57241 cccatcgttt tggcagcagc cagcgcaatc ggagccatca acaccgccgt cgcggtatta
    57301 gagataaaca gcccaataac cgccgacaag acaaacaaac accccagcat catatgtggc
    57361 ccataaccgc cgccaatatc catcagccct ttcaccgcca gcgcgacacc tcccgttttc
    57421 tgtaatgcca cagcaaacgg catcatccca acgatcaaaa taatgctcgg ccagtgaatg
    57481 gatttatagg cgctttcagc atctatacag cggaatttcc ccatcagcag gcaggcgatg
    57541 atagcggcaa caggattagg aatttcattt gtcagcatta acgccaccat tagcaccaga
    57601 cagaaaatgg catggggtgc ctggctgtgc gcgggcgatg cttcactcac ctcttccggt
    57661 aagttcagcg ctacgaagtc gcggccctgt ttggccagca taccgatcag tttccagtta
    57721 cccacaacca ggatgatatc gcccagcagc agaggctcat ccgccagcga accttccagc
    57781 gccacgccat tgcgcttgag ccccaccaca ttcagtccgt agcgggtacg aaaaccaatt
    57841 tcgcgcaccg atttaccaat cagttctgac tcaggaatta atgaaatctc tgccatgccc
    57901 acatcaaggg cctggtcaga aaaatactcg ccgcgcagta ccatcggctc cagcaattgc
    57961 tcactacaaa attgccggag atcgacatca gccgcagaca tatcaataag caaaacgtca
    58021 cgcgcgcgaa attcagaaac cccattaacg ttcacgataa cgcgacgaaa acgccgccag
    58081 cgttcaacac cgatgacgtt agcgccataa cgctcacgta atttgagatc atccagccgt
    58141 tgacctatca ttggcgatcc ggggcgaata gccagacgtc gcgcacgccc ggtcagtcga
    58201 tattcacgga taagatcgcg aaaggttcga cgcgtccagc cttcgcgctg cggggtctgg
    58261 gtatcccctt tcagcatgaa acgcatcact aacatataca aaatacccag caccagcacg
    58321 accaggccaa taggtgttac gctaaagaaa ctgaagccgt gatagccttc acgcagcaat
    58381 tcactgttga ctaccaggtt cggcggcgtc gccaccagcg tcatcatgcc gctaatcagc
    58441 ccggcaaaac ttaacggcat catcagacgt gacggcgaag tttgcatacg catggcaacg
    58501 cttaacacca cgggaataaa gatagcgaca acgctggttg aactcataaa cgcgccaagc
    58561 cccgcgacgg tcagcatcaa caaaaccaac attttgattt cactgttgcc cgccactttg
    58621 accagccatg ttcccattac ggtggcaaca ccggtacgga ccaaaccatc gccaataata
    58681 aacaaggcgg caatcaggac aacgttagga tcagaaaagc cggaaaatac ttcggggacc
    58741 gtcagcgttc cgcttaatgc aaacgcgaca ataacaaaca aagcgaccgc atccatacgc
    58801 actctgcccg tcgcaaacaa gacgatggca acggccagta atgaaagaac ccaaatcaat
    58861 tcaccgttca caacttatcc ttgttaattg agggggatga cttgattctg ccataaaaaa
    58921 gccccgacga gacggggcta aatcatgatc aggtgtttca ctgaataata acatcgccat
    58981 ttggctgttt ggtcacagta atttgctcca gactgtggag gaccaaatcg acctcattca
    59041 ggcgcggggt atctgccgga gcgttaaccg caatgacatg gcaacccgcc gctaggccag
    59101 aaagcacgcc agcgggagca tcttccacca ccacacactc ctgcggcgca agccccagca
    59161 gctgcgcacc taacagatac gcatcaggtt ctggttttcc gcgcttcact cgctcagcgg
    59221 ttacaaacac ctctggtgcg ggaagccccg ctattttatg gcgcgctcgc gctaccggca
    59281 tggagccaga agtcacaatg gcccacggaa tacctgcttt attcaaatga ttgagtaagg
    59341 cgattgcccc agaaagcgcg gtaatacctt cggtttccgt ggcctcgatt tgctccagac
    59401 gcgtaaactc ggcggcaata tcagcctcgg atttgcccgc cataaaatgg cgcaaagagg
    59461 tgatcgcctg tttaccgtga atgaaagcca gcacctcttc cggcgctaac ccatgacgtc
    59521 tggcccagtt gctccacgcc cgttctaccg caggcaggga atccaccagc gttccatcaa
    59581 gatcaaacag aaaacctttg caccgcacgc gggcctcctc aggcgttgat aatttgatta
    59641 atttcgttgg cgctcaaatg gtactgacgc gggcaggcat gccacacatt aagcatgcgc
    59701 tgatattttt cccacattgg cgtctgggcg ttaaaaccat gagtcccagc gtcaaaatgg
    59761 gtatagcgcc cttccacatt aaccataaag cggacataac cgaggtaacg tgcttcagtg
    59821 gcggcgtcaa agccgaggaa ggtgacacga cgttcatcga tggattgctg gtcctgcaaa
    59881 ttagaccagg aaacatgcaa cgcatgatac atctccataa tgtcgatgat agtgcggcag
    59941 gtttcttctt tcagctcgcc aaactcgcga tccaattcac gcatctgtaa tccgtaacca
    60001 cgctcaataa ttgtttgcag gcgacggtaa cgttcagcat ttgccggatc gagcatagtc
    60061 atcatcttgt actgattaga caaaataaga cgttgcgcgt tggtcatttc cattgttgac
    60121 tcctgtatca ctctactacg gtgaaaaaaa agaaggctga gtatgccttc ttttatatgc
    60181 gtaatcaggg gtcaattaca aatcatcaag gaaagtttta tccagttgtt tgaaggcgcg
    60241 cttaagcgtg tcagctaatg cctggtaatc aggcttgcct tcaacgggtg ccaacacctg
    60301 tccagactcc tgcaatttac cgcgaacttc ataaaaccag ttaaggattg cagggggtaa
    60361 tggcgttaca gaacgcttgc ccagccacca caatccctgc atgggtaaac ttaaggcgaa
    60421 cagtgcagtg gcaactgccg gcccaagcag accgcccagg gcaatctgcc agcagagagt
    60481 aaatacggcg atcggcggca taaaacggat cgcataacgc gtcatcttga taacgcgatt
    60541 ttcgacaaag accggggcaa ggcgtttttc cagtggccac gtctttgagt aatgctgtcc
    60601 gcggcgaaac aagctaaaaa aattaacaga acgattatcc ggcgttgaca tgcttcacct
    60661 caacttcaca tataaagatt caaaaatttg tgcaaattca caactcagcg ggacaacgtt
    60721 caaaacattt tgtcttccat acccactatc aggtatcctt tagcagcctg aaggcctaaa
    60781 tagtacatat tcattgagtc gtcaaattca tatacattat gccattggct gaaaattacg
    60841 caaaatggca tagactcaag atatttcttc catcatgcaa aaaaaatttg cagtgcatga
    60901 tgttaatcat aaatgtcggt gtcatcatgc gctacgctct atggctccct gacgtttttt
    60961 tagccacgta tcaattatag gtacttccat gtcgagtaag ttagtactgg ttctgaactg
    61021 cggtagttct tcactgaaat ttgccatcat cgatgcagta aatggtgaag agtacctttc
    61081 tggtttagcc gaatgtttcc acctgcccga agcacgtatc aaatggaaaa tggacggcaa
    61141 taaacaggaa gcggctttag gtgcaggcgc cgctcacagc gaagcgctca actttatcgt
    61201 taatactatt ctggcacaaa aaccagaact gtctgcgcag ctgactgcta tcggtcaccg
    61261 tatcgtacac ggcggcgaaa agtataccag ctccgtagtg atcgatgagt ctgttattca
    61321 gggtatcaaa gatgcagctt cttttgcacc gctgcacaac ccggctcacc tgatcggtat
    61381 cgaagaagct ctgaaatctt tcccacagct gaaagacaaa aacgttgctg tatttgacac
    61441 cgcgttccac cagactatgc cggaagagtc ttacctctac gccctgccgt acaacctgta
    61501 caaagagcac ggcatccgtc gttacggcgc gcacggcacc agccacttct atgtaaccca
    61561 ggaagcggca aaaatgctga acaaaccggt agaagaactg aacatcatca cctgccacct
    61621 gggcaacggt ggttccgttt ctgctatccg caacggtaaa tgcgttgaca cctctatggg
    61681 tctgaccccg ctggaaggtc tggtcatggg tacccgttct ggtgatatcg atccggcgat
    61741 catcttccac ctgcacgaca ccctgggcat gagcgttgac gcaatcaaca aactgctgac
    61801 caaagagtct ggcctgctgg gtctgaccga agtgaccagc gactgccgct atgttgaaga
    61861 caactacgcg acgaaagaag acgcgaagcg cgcaatggac gtttactgcc accgcctggc
    61921 gaaatacatc ggtgcctaca ctgcgctgat ggatggtcgt ctggacgctg ttgtattcac
    61981 tggtggtatc ggtgaaaatg ccgcgatggt tcgtgaactg tctctgggca aactgggcgt
    62041 gctgggcttt gaagttgatc atgaacgcaa cctggctgca cgtttcggca aatctggttt
    62101 catcaacaaa gaaggtaccc gtcctgcggt ggttatccca accaacgaag aactggttat
    62161 cgcgcaagac gcgagccgcc tgactgcctg atttcacacc gccagctcag ctggcggtgc
    62221 tgttttgtaa cccgccaaat cggcggtaac gaaagaggat aaaccgtgtc ccgtattatt
    62281 atgctgatcc ctaccggaac cagcgtcggt ctgaccagcg tcagccttgg cgtgatccgt
    62341 gcaatggaac gcaaaggcgt tcgtctgagc gttttcaaac ctatcgctca gccgcgtacc
    62401 ggtggcgatg cgcccgatca gactacgact atcgtgcgtg cgaactcttc caccacgacg
    62461 gccgctgaac cgctgaaaat gagctacgtt gaaggtctgc tttccagcaa tcagaaagat
    62521 gtgctgatgg aagagatcgt cgcaaactac cacgctaaca ccaaagacgc tgaagtcgtt
    62581 ctggttgaag gtctggtccc gacacgtaag caccagtttg cccagtctct gaactacgaa
    62641 atcgctaaaa cgctgaatgc ggaaatcgtc ttcgttatgt ctcagggcac tgacaccccg
    62701 gaacagctga aagagcgtat cgaactgacc cgcaacagct tcggtggtgc caaaaacacc
    62761 aacatcaccg gcgttatcgt taacaaactg aacgcaccgg ttgatgaaca gggtcgtact
    62821 cgcccggatc tgtccgagat tttcgacgac tcttccaaag ctaaagtaaa caatgttgat
    62881 ccggcgaagc tgcaagaatc cagcccgctg ccggttctcg gcgctgtgcc gtggagcttt
    62941 gacctgatcg cgactcgtgc gatcgatatg gctcgccacc tgaatgcgac catcatcaac
    63001 gaaggcgaca tcaatactcg ccgcgttaaa tccgtcactt tctgcgcacg cagcattccg
    63061 cacatgctgg agcacttccg tgccggttct ctgctggtga cttccgcaga ccgtcctgac
    63121 gtgctggtgg ccgcttgcct ggcagccatg aacggcgtag aaatcggtgc cctgctgctg
    63181 actggcggct acgaaatgga cgcgcgcatt tctaaactgt gcgaacgtgc tttcgctacc
    63241 ggcctgccgg tatttatggt gaacaccaac acctggcaga cctctctgag cctgcagagc
    63301 ttcaacctgg aagttccggt tgacgatcac gaacgtatcg agaaagttca ggaatacgtt
    63361 gctaactaca tcaacgctga ctggatcgaa tctctgactg ccacttctga gcgcagccgt
    63421 cgtctgtctc cgccagcgtt ccgttatcag ctgactgaac tggcgcgcaa agcgggcaaa
    63481 cgtatcgttc tgccggaagg tgacgaaccg cgtaccgtta aagcagccgc tatctgtgct
    63541 gaacgtggta tcgcaacttg cgtactgctg ggtaatccgg cagagatcaa ccgtgttgca
    63601 gcgtctcagg gtgtagaact aggtgcaggc attgaaatcg ttgatccaga agtggttcgc
    63661 gaaagctatg ttggtcgtct ggtcgagctg cgtaagaaca aaggcatgac cgaaaccgtt
    63721 gcccgcgaac agctggaaga caacgtggtg ctcggtacgc tgatgctgga acaggatgaa
    63781 gttgatggtc tggtttccgg tgctgttcac actaccgcaa acaccatccg tccgccgctg
    63841 cagctgatca aaactgcacc gggcagctcc ctggtatctt ccgtgttctt catgctgctg
    63901 ccggaacagg tttacgttta cggtgactgt gcgatcaacc cggatccgac cgctgaacag
    63961 ctggcagaaa tcgcgattca gtccgctgat tccgctgcgg ccttcggtat cgaaccgcgc
    64021 gttgctatgc tctcctactc caccggtact tctggtgcag gtagcgacgt agaaaaagtt
    64081 cgcgaagcaa ctcgtctggc gcaggaaaaa cgtcctgacc tgatgatcga cggtccgctg
    64141 cagtacgacg ctgcggtaat ggctgacgtt gcgaaatcca aagcgccgaa ctctccggtt
    64201 gcaggtcgcg ctaccgtgtt catcttcccg gatctgaaca ccggtaacac cacctacaaa
    64261 gcggtacagc gttctgccga cctgatctcc atcgggccga tgctgcaggg tatgcgcaaa
    64321 ccggttaacg acctgtcccg tggcgcactg gttgacgata tcgtctacac catcgcgctg
    64381 actgcgattc agtctgcaca gcagcagtaa tctcgtcatc atccacagct ttgcgctgcg
    64441 gatatctgaa ccggaaataa tcactatttc cggtttttta ttctcttaat ttgcattaat
    64501 cctttctgat tatcttgctt aactgcgctg catcaatgaa ttgcgccatc tcactttgca
    64561 tacttaccac tttgttttgt gcaagggaat atttgcgcta tgtccgcaat cactgaatcc
    64621 aaaccaacaa gaagatgggc aatgcccgat acgttggtga ttatcttttt tgttgctatt
    64681 ttaaccagtc ttgccacctg ggtagttccg gtggggatgt ttgacagtca ggaagtgcag
    64741 tatcaggttg atggtcaaac aaaaacacgc aaagtcgtag atccacactc atttcgcatt
    64801 ctgactaacg aagcaggcga acctgagtat caccgcgtac agctgttcac gacgggcgat
    64861 gaacgcccgg gcctgatgaa cttcccgttt gaagggttaa cctcaggatc gaaatacggg
    64921 acagccgttg gcatcatcat gtttatgctg gtgattggcg gcgcgtttgg cattgtgatg
    64981 cgtacaggaa ccattgataa cggtatcctg gcgcttattc gccatacccg cgggaatgaa
    65041 attctcttta ttcctgcgct gtttattctg ttttcacttg gcggcgcggt atttggtatg
    65101 ggagaagagg ccgtcgcctt tgccattatc atcgcaccgc taatggtccg gctgggctat
    65161 gacagtatta ccaccgtcct ggtgacttat attgccacgc aaatcggttt tgccagttcg
    65221 tggatgaacc cgttttgtgt ggttgttgct caggggattg ccggcgttcc ggtgctttct
    65281 ggctccgggt tgcgcatcgt ggtgtgggtt atcgccactc tgattggcct gatctttacc
    65341 atggtgtacg cctcacgagt gaaaaagaat cctcttctgt cacgcgtgca tgagtccgac
    65401 cgcttctttc gtgaaaagca ggcggatgtt gaacaacgtc cgtttacctt tggtgactgg
    65461 ctggtattga ttgtcctgac agccgtaatg gtctgggtga tttggggcgt gatcgttaat
    65521 gcctggttta ttccagaaat tgccagccag ttcttcacca tgggtctggt gattggcatc
    65581 atcggcgttg ttttccgcct taacggcatg acggttaata ccatggcttc atcctttacc
    65641 gaaggggcgc gaatgatgat cgcccctgcc ctgctggtgg gtttcgccaa agggattttg
    65701 ctgctggttg gtaatggtga agcaggtaat gccagcgtgt taaatactat cctcaacagc
    65761 attgccaatg ccattagcgg tctggacaac gcggtcgcgg cctggtttat gttgctcttc
    65821 caggctgtat ttaatttctt cgtgacgtcc ggttctggtc aggcggcatt aactatgccg
    65881 ttactggcac cgcttggcga tctggtcggt gttaaccgtc aggttaccgt gctggcgttc
    65941 cagtttggtg atggcttcag ccacatcatt tacccaacct cagcttcgtt aatggcaacg
    66001 ctcggtgtgt gccgggtgga cttccgtaac tggctgaagg tgggcgcgac cctgcttgga
    66061 ctgctgttta ttatgtccag cgtcgtagtg atcggcgctc agttgatggg ctaccactaa
    66121 aaatgttaag agccgcaatt gcggctcttt tttcattctg ccgtttcagt ctctacggct
    66181 tcatttttgg cattgcgctt catccacaac gccagcgctt tcagcgagtc tggagtgaac
    66241 tcatcgcagc gtgcggtgat ttcttccggc gtcagccagc aaacttcact gacttcatct
    66301 tcctgtaggg cgaagggacc gtgagagacg caactgaaca acgcgcccca gacacggcaa
    66361 tttttatctt cgaaatagaa ctgcccgtgc tcggcaaagg ggacaccggc aatgcccaac
    66421 tcttcttccg cttcgcgacg cgcggattcc agcagttgct catcggcctg aactacaccg
    66481 cctgcggtcg catctaacat gccgggtaaa aagtcttttg tctcggtacg acgctggacc
    66541 agaattttgc ccatgccatc atgcacgacg atgtaagttg cacgatgacg cagacactgt
    66601 gcccgcattt gttcccggct ggcttgtgca atgacttcgt tctcttcatt gacaatatcc
    66661 acccattcag tacttgccaa acgacgctgt tccattatca ggaaaccttc tttttctggc
    66721 gctcttacgg cgcattttga gttgtgggta aattacggat taatcgcgac ctgcgcaatg
    66781 atactttgat cattgagtgc gattacacta agtacgtcat tatccagcat gccataactc
    66841 gccggattac cgcctttcgg aatactcacc gagccggggt tgaagtggaa aatctccccc
    66901 cgctgttctg ccactggcag atgggtatga ccgtacacca gcacatcgtt ctggtttaaa
    66961 gcaggtagat tttccgggcc aaaaagatga ccatgcgtca aaaacagacg ttgtttttcc
    67021 agtaatacct gttgccacgg cgcggttatc gggaaatgca gcatcatttg atccacttcg
    67081 ctgtcgcagt tgccacgcac agcgataacc ttatgtgcca cttcattaag ccgttcagcg
    67141 actttggctg gcgcgtaacc ctccggtaaa gcattacgcg ggccgtgatt caacacgtcg
    67201 ccaaggatca ccagccactg ggcaccgctt tggacaaaca actccacaac acgttccgtc
    67261 gccggtaacg acccatgaat gtccgatgca aacatcagtt tcatcactca ctcctcgtcg
    67321 aaaaaacgta agctatgata ctggattctg tcgttgctat cagccagatt gcttcgccga
    67381 aagtgcaata aaacgctgga cagacgctcg ctgccactgg aatgtcgcat aatccaccag
    67441 gcgttctggc acctcatcgc catgtagtac caggcggtta atcattagcg ccagatcagt
    67501 atcagcaatg caccattcac caaataaatt cggctgaccc agtgctaaca aatgttctgc
    67561 catcgcgaac agtttctctg cactggcttt tccctcggcc gttagtggcg cttttttcgc
    67621 ccccgcaaag acaacatccg tcggacgctc ttcgcggatg ggcatcagat cgctgcgcag
    67681 ccaggcctga atctgtcgtg cacgcgcacg attttctaaa tcaagcggat aaatacgttc
    67741 ccaggtcggt ggcgcaaatc gatcttccag atactccgca atggcagaag attcactcaa
    67801 ctcaaaatca tcgatttgta ataacggcac acggcgtgtc tgaccgtaac cttgccacgt
    67861 cggctgcaaa tgttcaccgc tgtcgaggtc gatggtcttg atatgaaacg acaggccttt
    67921 ttcctgcaac gccacccagg cggataacac ataaggggag aaaaagtggg catctgacca
    67981 aagcgtgata gcgggtttac tcattatgtc ctcggcaata aagggggctg gctatcaaac
    68041 tatagagcct ttttgtcact gtcacgtgac gaatactcac gtaagtcgcc gcgctttcta
    68101 tacttgtggt gttcagatga ttaaacaacc ggagctgcaa tgatcgatct ctatttcgcc
    68161 ccgacaccca atggtcacaa aattacgctg tttctcgaag aagcagagct tgattatcgc
    68221 ttgattaagg tagacctggg gaaaggcggt cagtttcgtc cggaattttt gcgcatttcg
    68281 cccaacaaca aaattccggc aattgttgat cattctcctg ccgatggcgg cgaaccgtta
    68341 agcctctttg agtctggtgc cattttgttg tatctggctg agaaaacagg actctttttg
    68401 agtcatgaaa cgcgtgagcg cgccgccaca ttacagtggt tattctggca ggtaggcgga
    68461 ctggggccga tgcttgggca aaatcatcat tttaatcacg cagcccccca aaccattcct
    68521 tacgctattg aacgttatca ggttgaaact cagcgtcttt accatgtact aaacaagcgg
    68581 ctggaaaact cgccctggct gggaggcgag aactacagca ttgcggatat tgcctgctgg
    68641 ccgtgggtta atgcctggac tcgcctgcga attgacctcg caatgtatcc ggcagtcaag
    68701 aactggcatg agcggatccg ttcgcgccct gccaccgggc aggcactgct aaaagcacaa
    68761 ctcggtgatg agcgttcgga tagttaacag aaacaggttc tcgtgtatta tttcatccta
    68821 agtaaaacaa cggagaacct gcaatggcac aacctgccgc tattattcgt ataaagaacc
    68881 ttcgtttgcg tacgtttatc ggaattaagg aagaagaaat taacaaccgt caggatattg
    68941 ttatcaatgt gacgatccac taccccgccg ataaagcgcg cactagcgaa gatatcaacg
    69001 atgcgctgaa ttatcgcacc gtaacgaaaa acattattca gcatgtagag aataaccgtt
    69061 tctctttgct ggaaaaatta actcaggatg tgctcgatat cgcacgtgaa catcactggg
    69121 tgacgtatgc tgaagtggag atcgataaac tgcacgcgct gcgctacgcc gattcggtat
    69181 ccatgacctt aagctggcag cgttaatcgc catatcggga ggctgcatga acatagtgat
    69241 caccggaggg acgggattaa ttggtcgcca tttgattccg cgtttgctgg agctgggcca
    69301 tcaaattacg gtagtgacgc gtaacccgca gaaagccagt tccgttctcg gccctcgggt
    69361 gacactatgg caagggcttg ccgatcaaag caacctcaac ggcgttgatg cggtaatcaa
    69421 cctggccgga gaaccgattg ctgataaacg ctggactcac aagcaaaaag agcgtctctg
    69481 ccaaagccac tggaatatca cgcaaaaact ggtcgatttg attaatgcca gcgacacgcc
    69541 gccgtcggta ctcatttccg gctcggcaac gggctattat ggcgacttag gtgaagtggt
    69601 ggttaccgaa gaggaaccgc cgcataacga atttactcat aaactctgcg cccgctggga
    69661 agagattgcc tgccgggcgc aaagtgacaa aacgcgagtg tgcctgctgc gaaccggcgt
    69721 ggtactggca ccggacggcg gtattctcgg taaaatgctg ccaccgtttc gtcttggcct
    69781 gggcgggccg attggttccg gtcggcagta tctggcctgg attcatattg atgatatggt
    69841 caacggcatt ctctggctgc tggataacga gctgcgcggg ccatttaata tggtttcgcc
    69901 ctacccggta cgcaatgaac aatttgccca tgcgctcggt catgcgctgc atcgcccggc
    69961 tattttgcgc gtccccacaa ccgccattcg gctgttaatg ggcgaatctt cagtactggt
    70021 attaggtgga caacgcgcgc tgcctaaacg gctggaagaa gcgggttttg cgtttcgctg
    70081 gtacgattta gaagaggcgc tggcggatgt cgttcgctga tgtggtttac agcaaacatc
    70141 cgccagttaa ctccctgtgt tacaggatta gtggctttgc gcgataagat cgtctggtga
    70201 aagtcgggtc accatcataa ctaactctct gtctaaacct ctatccagca tctcctgagc
    70261 aatacgcagg gcttcttcgt gtttgccctg cattgcgcct tcttcacgta atctgtcagc
    70321 aatggtcatc agtttctcct tttcttgtgg tgcgcgttct gttatctcac caataaatgc
    70381 acggaaacgc tgggcatccc ctgtttgtaa tacgtaatta aacagggctt ttagctgtct
    70441 gtcattagtg ttccctgtaa ctagcagcga aacaatttgg tcgactaatc ccaacagatc
    70501 gcgctgacga atatgtttct gaattaactc caacagcgcc attttgcggt gttgcataat
    70561 ctcgtcatcc ggcaccacgg caatatccac caacggaaac gctgatgaat atattttgcg
    70621 ggctatagca ggctcggcaa attcatcaag ccagcagagt gaataaggat aaggacttct
    70681 gcaaccatga taaaacagca tcgggatcac caatggaagc tctttatagc ccgcatcaag
    70741 atggttttgc attgccgcaa tggaataacg catcatgcga aaagccatta attcttccgg
    70801 cttactttgg tgctctatca ctacataaat ataacccact ccctcctgcg ttttcacaga
    70861 ccacaagagg tcggaataat attgccgcag gtcttcatca ataaaactgt ttggttctag
    70921 tttaagcgtc gttaaatcac acagtttgcg cagcggcgcg ggaagatgaa tattaataaa
    70981 attccgcgcg gtgtctggat ggcgtaaaaa agatttaaat accgcatcat gcggcgtgga
    71041 agttgtcgat attgtcatgg cgttccgtca cccctcaaat ttaagatgac gcgacaataa
    71101 ccacgattaa cacccaatgc agcaggcagt ttttatcttt ggacagcgaa ttcagaagta
    71161 tttttagcgg actgcaactt ttacatttca gcggaagctg actcgcaaat tccccttgaa
    71221 aatactccgg cagcgttagc caccggagag gaaattattt cagcgatccc ttaaggaact
    71281 gttgcagacg agggctttgc gggttgccaa ataactgctc cggcgcgccc tcttcttcta
    71341 ttttcccctg atggaggaaa atgacgtgag tagaaacatg gcgagcaaag cccatttcgt
    71401 gagtcactac caccatggtt ttcccctctt ctgccagttg ctgcataata cgtaacactt
    71461 cgcctaccag ttcaggatcg agcgccgagg taggttcatc aaacagtaaa acttctggtt
    71521 ccatcgccag cgcccgcgcg atagaaacac gctgttgctg accgccggaa agatgcaccg
    71581 gatatttccc ctgcgcacgt tcgtctatcc cgactttcgc cagatacttc accgcccgct
    71641 cgcacgcttc ctgcttgctc aggcccaaca cctgaatcgg cgcttccatg acgttttcca
    71701 acaccgtcat atggctccag agattgaagt gctggaatac catcgtcagg cgtgtgcgca
    71761 gtaagcgcag ttgattttta tcggcgactt tgagttgacc gtctttgtcg cgcaccagat
    71821 tgatcgtctg gccattgacc acgatcgatc cttcactcgg tttttcgagg aagttaatgc
    71881 agcgcagaaa ggtacttttc cccgatcccg acgatccgat gatgcttatt acatctccgg
    71941 cattcgcttg cagtgagacc cctttcagca cttcgtgttc gccatagcgt ttgtgcaaat
    72001 cgataacgtt taatttattc tcggacatcg tattctcagt gcgttgaaga aggtttcaca
    72061 tgctgcaacc agcgtttttc cgctctgcga aagaggctga tcaggacata agagatgatt
    72121 aaatagagca ccgcggcaat gccgaaggcg gtaaaaggtt gatacgtggc ggcgttaata
    72181 tcgcgggcta ttttcagcag atccggcaca gtggccgtaa acgccagcgc agtagagtgc
    72241 agcatcagga tcacttcgtt gctgtatgcc ggtaacgcaa tacgcagcgc agaaggcaaa
    72301 ataatgcagc gatacatttt aaaggtcgag aagccatagg ctctggcggc ttcaatttcc
    72361 ccatgcggaa ccgaacggat tgccccagca aaaatctcgg tagtgtaagc gcaggtgtta
    72421 agcgtcagcg ccagcacggt acagttcagg ccactgcgga agaaagcgtt aaggaattcg
    72481 gttcccttaa caatctcaag cgtgtacatg ccggaataga acaccagcaa ctgaacatac
    72541 agcggcgtac cgcgaaaaat ataggtaaat aaccagattg gaaactggat gtatttatta
    72601 ctggagacac gaccaatcgc cagaaacagc gccaggactc cgcctatcac taccgacaaa
    72661 ataagcagcc acagagtgat cgccacacca gtaaagcgat aaccgtcggt ccacagcagc
    72721 ggtttccagt attcatgtaa gatttcgatc acaggtcagc cctcttcaca cccacggagt
    72781 agcggcgctc aaggaacagc agcacaccat tggaaacggt ggtgaaaacc aggtaaatca
    72841 cgccacagac gatggcgaaa tagaacggtt cccaggtact tttgcctgcc agctgcgtgg
    72901 ctttgaccac atcttccagg ccgagtaacg aaaccagtgc ggtagatttg aggatcacct
    72961 gccagttgtt gccaatgcct ggcagggcgt aacgcatcat cgccggaaac atgatcctcc
    73021 gaaacacttg cccgcgagta aaaccgaacg ccgtcgccgc ctctatatgc cctttcggca
    73081 ctgccataaa cgcgccacgg aacgtttcgg taaaataagc accgtagata aaaccgagag
    73141 tgataatacc agcgaccatc ggatcgatat caatctgccc gacgcccatc gcctccgtca
    73201 ccatgtttag cgcaatctgc aaaccataga aaatcagcag catcaacacc aagtccggca
    73261 cgcctcgaat cagcgtggtg tacccttcga aaatcagccc caaaagccga ttttgcgaga
    73321 gcttaccgcc agcgccaatt aaaccgatga ttacagcgag cactacagag ctgatagcca
    73381 gctccagcgt gacgagcgca ccctgtaaaa taacacctga aaacccatac aacatgctgc
    73441 ctgtcctgtc gtgtgtggtg aattaccgcc tcttgtctcc ctccggttac ccgaagggag
    73501 cgggctttct ttcaccgatg gcgattaacc accataaaca tcaaaatcga agtacttttt
    73561 cgctaatttc tcgtaagtac cgtcagcgcg catttcggca aaggctttgt tcagtgcctc
    73621 gcgcagttcg ttatcttctt tacgcaggcc catgccggtc cctacgccaa acagtttttc
    73681 atctttaaca gacggaccac cgaatttgta atctttaccg acgggttgtt tgaggaaacc
    73741 ttcgctggca gcgacctcat cctggaacgc ggcatcaata cgtccggcag tcaggtcaga
    73801 ataaatgttg tcctgcccct gatacgagac gatttcaatg ccttttggtg cccagtgttc
    73861 attaccgaac gtctcctggg tggtgccctg caatacgcct acccgtttgc ctttcagcga
    73921 ctcgactgtc ggctgaatgt cagaattttt cgccaccacc agacgagaat cggcagcgta
    73981 cagtttgtcg gtgaaggcaa tttcttgctg acgtttttcc gtaatggaaa gcgatgacat
    74041 gatggcgtca atcttcttcg cttttaagga cgggattaac gcatccagcg ggttttcgac
    74101 aaacgtacat tgcgtattga tgcgcttgca caactccttc gccagctcga tatcgaaacc
    74161 aaccagttcg ccttgtgaat tttttgattc aaatggcgca taggtcgggt cggtaccgat
    74221 gcggatgttt tgcggaattg cagcaagtgc cgcagttgcg ctggagaagg ccagaaccag
    74281 agagagcgat agcaccagtt ttttcataat gtcctcaact gacagtcttt ttatagggaa
    74341 tatttacagg tttgttacac ttatcgtgcc ataaaattga ccatcaaggc aaaatatccc
    74401 gccccgttag acgtattatt tttcattaaa aatgcttaag tatgcatttc aatgcaccac
    74461 gatagtgcaa caaacctggc gtgcaccatg atgatcattc catcaggtac agcttcccag
    74521 cgacgtatca gtcaccgtag acattaaagt cgaaatactt tttcgccatc ttgtcgtagg
    74581 tgccgtcctg acgcagctcg ccaagcgcct tattgaaggc agccgtcagt tcagcatcat
    74641 ctttacgtag ccctacaccg gtgccatcac cgaagtattt tttgtctttt actgatgagc
    74701 cagcaaaggc gaaatcttta ccagcaggtt gcttgaggaa tccttcgctg gcagcaactt
    74761 catcttgtaa cgcagcatcc agacgtcctg cagccagatc ggaatagacc aaatcctggt
    74821 tggcataggc caccacatcc acgcctttac tacgccaggt ctcgttagcg taagcttcct
    74881 gggttgatcc ctgcagcaca ccaacatgtt tacctttcag tgaatccagc gttggctgaa
    74941 tcggtgaacc tttggcagca atcaaacgag aatctgcggc gtacagcttg tcggagaagg
    75001 caatctcctg ctgacgttta tcggtaatgg aaagcgacga aataatagcg tcgatttttt
    75061 tcgctttcag tgaggggagc agcgcgtcaa agtcactggc aacccaggta catttcacct
    75121 gcatccgttt gcacatctcg ttaccgagat cgatatcaaa gccaacaaaa tcacctttag
    75181 catctttcga cgagaacggc gcataggtgg tatcggttcc gatacgtacc gtctccggta
    75241 gcgccgcata gctggaagcc gctgcggaga gaccgactaa taaagacaga gcgagaatcg
    75301 acttcttcat acataaccct caagtgaaaa tggctttttt atgttgtgta ttgtgttgtg
    75361 tgtttgcagg ctctttcatg caggtcttat gccatcttga cgacaacagt aacattcaac
    75421 gttaaatatg ttaataagac gttgcattat tgtcctgaag ttgaagatag caggtatagc
    75481 ggttgaatcg cagcgtttcg attgaatggc agaaacaaaa tgtcgaggat ttgatcgcgg
    75541 ttgcaaaatt gccctgaaac agggcaacag cggagttatg cgccctgcca gcgggcaaag
    75601 agatcttcag gaagggttat tgcaaactgg tcaagaacac gattaaccgt ctgatttatc
    75661 acatcatcaa gggattgcgg gcgatgataa aacgccggaa cgggaggcat aatcaccgca
    75721 ccgatttctg ccgcctgagt cattaaacgc agatggccta agtgcaatgg tgtttcacgc
    75781 acgcagagca ccaacgggcg acgctctttc agcaccacat ctgccgcacg ggtcagtaag
    75841 ccatcagtat agctatggac aatgccggac agggttttga ttgaacaggg taaaatcacc
    75901 atccccagcg tctggaaaga accggaagag atgctggcgg aaatatcgcg cgcatcgagc
    75961 gtgacatcag ctaatgcctg cacttcgcgc agggaaaaat ccgtttcgag ggataaggtc
    76021 tggcgcgccg cctggctcat caccagatgc gtttcgatat ctgtgacatc gcgcagaacc
    76081 tgtaataagc gcacgccata aatcgcgccg ctggcaccgc tgatgcctac aatgagtcgt
    76141 ttcataaata gttgccctgt cagacttgca ggcagacttt gcagtatttc ggcgggagtt
    76201 gcaagtcagg gtgccagacc ggcaccctca gcgaaggcat catccttcgt tatgcatttc
    76261 gagattttcc acttcgttct gacgttgcac tgctttggcg tcatcattac gtaacgtatc
    76321 gaggaaatcg aggtagccct gatcaacatc tttggtgacg tagacgccgt tgaacaccga
    76381 gcattcaaac tgctggatat ccggattttc agcgcgaacg gcgtcgatca gatcgttcag
    76441 atcctggaaa atcaacccgt cagcaccgat gatctggcga atttcatcaa cttcgcgacc
    76501 gtgagcgatc agttccgtgg cgctcggcat atcaatacca taaacgttcg ggaagcgaat
    76561 ttccggtgcc gcagaagcga ggtacacttt cttcgctccg gcttcgcgtg ccatctcgat
    76621 aatctgctca gaagtggtgc cacggacgat ggagtcgtcg accagcagga cgtttttatc
    76681 gcggaactcg gcgcggttgg cattcagttt acggcgcacg gacttacgac gcagctgctg
    76741 gcccggcatg ataaaggtgc ggccaacata gcggttttta acgaagccct ggcggtacgg
    76801 tttgcccaga atacgagcaa tttccagcgc gatatcacac gaggtttctg ggatcgggat
    76861 caccacgtcg atatccagat cttcccattc gcgggcaatt ttctcgccca gtttcgtgcc
    76921 catattcaca cgcgcgctgt aaacggaaat tttgtcgata aacgagtccg ggcgggcaaa
    76981 gtatacatac tcaaacaggc acggattgct gaccggattg tcagcacatt gacgggtaaa
    77041 caactgccct tcttcagtga tgtaaatcgc ttcgcccggc gcgacgtcac gcaggaaatc
    77101 aaagcccagc gtatcgagcg ctacgctttc ggaagcgacc atatattctg tacggttctc
    77161 gtcaatatca cgttttccca gtaccagcgg acgaatcccg tttggatcgc ggaaagcaac
    77221 cataccgtgg ccgataatca tcgccacaca ggcatacgcg ccgcggatta agcggtttgt
    77281 ggcagcaatg gcagcgaaaa tattgtcggc ttccagcggg tagtggcgga agttgtccag
    77341 ctcgctggcg aagatattaa gcagaatttc cgagtcggaa gtggtgttga tgtggcggcg
    77401 tttttcttca aacagttttt tacgcaactc gtgagcgttg gtcagattgc cgttgtgggc
    77461 aagcgtaatg ccatacgggg agttaacgta aaacggctgc gcttcagagg cgctggagct
    77521 gccagccgta gggtaacgca catgaccaat gcccatattg ccctgcaaac gctgcatatg
    77581 gcgagcttca aatacatcgc tcaccagccc gttcgcttta cgcaaacgga agcagttatt
    77641 ggcatctatg gtgatgatgc cggcggcatc ctgaccgcga tgctgaagca ccgttaaggc
    77701 atcataaatc gactggttaa ccggcataac accggcgata ccgacaatac cgcacatacg
    77761 tctttttcct cgttaagcca catctcagag cacttacgct ctgggcaaga aacttgacga
    77821 gctttgcaga taatcaaaaa accatctgat gataaaactg aattgcggga tcagctgtga
    77881 tttgctccag tcttcacttt tcgacacccc ggtaaaggag tcgagaaaga agagaatggc
    77941 ggcaacaatc aacacaccgc gcaacgcacc gaaacagacg cccagcaccc gatcggtgcc
    78001 tgacaacccc gttttctcca ccaactggcc tatcacgaag ttcacgatag caccaacgat
    78061 cagggtagcg ataaacagta ccgcgatggc aatcccattt cgaaccagtt cgtcttcaaa
    78121 gcccgtaaac cagactgaca ggtaagtgta gtaatgactg gcaacaaaga aagcacaacc
    78181 ccatgtcacc agcgataacg cttcacgaac aaagccgcgg atcaggctaa ccagagagga
    78241 aaaagcaatc accgcgatta tggcgtaatc aatccagacc atatgtgtcc cacgatttta
    78301 cgccctgtca tcctgttcgg ggcgcattct aacagaaaaa gaaaacgttt gcgtagggat
    78361 ttccttcccg cgcatcaata aaaatggcgc tgaaaaaata ttcaacgcca tcgacttttt
    78421 atgcctttgc ggcatcgtgc aatgcgtgtc ggatgcggcg taaacgcctt atccgaccta
    78481 cggttctaac actgcccaag cctgataaga cgcgccagcg tcgcatcagg caagactgtg
    78541 ttaattcggc gtatagccca ttaccacgcc acttaagcca gaaagttgct tcaactcacc
    78601 cagcgaacct ttcagcttat ctttcgaggc atccggccca accagaatac gggtaatttt
    78661 accctgcact ggcgtggatg gcgacgtata aacccgataa ccggcaccgc gcagcttacc
    78721 gacaatctca ttcactttat cggcattttt cagcgcaccc agttgcacaa cataggcttt
    78781 acccgttggc gctgcttttt cttccacgac cggcttcggt tctggcttcg gcgcaggtgg
    78841 tgcttcaacc tttggtttag gcggctccac cggtttcggc ttcggtgggg cgaccggtgc
    78901 aggttccggt tcaaactcgg tgttattggc tgcaatagtg gccggatcga gcgacggtgc
    78961 tgcggcatca cctgcccgca cctcttccgc tgcgccttcc ggcggctgcg tcggcaacgc
    79021 ctgggtggcg gctggcatca tatcaggctc atcacgatcg cccgctttcg gcaccagcgg
    79081 gatagccgcg aactcatcct gataatgttt tttctgcccg tccagcagcc ctggaagtac
    79141 aatcaccccc agcgccacca gcacgatcgt gcccactaac cgattctgaa acttacttgc
    79201 caccgcttct cctcgcgtca atcacttcca tgacatgtgc gaccgtgtgg aaagaaccac
    79261 acaccagcac ggtgtcttcc gctttagcgt ccgccattgc ggcatcccat gcctgcgcaa
    79321 cgctatcaaa tgatttgccg ttacccaaat gctcaagcag ttgttctgcc gtggcaccgc
    79381 gcggcccttc cagtggcgca caataccagt catcaaccac gcttttcaac caggccagag
    79441 ttccggcaat atctttatca tgtagcatac cgataaccgc cagcacgcgc ccgtttttcg
    79501 gtagcgcttt catacgcccg gtgagatatt ccgccgcatg tggattatgc gcgacatcaa
    79561 aaataacgcg tggcgactcg ctcacaatct ggaaacgtcc cggcaaaatt gcgctggcaa
    79621 tcccgtcgcg aatggcattt tcactgactt ccagcccgct ggcacgcagt gccgccagcg
    79681 ccgttgcggc attcggttgc gggacaagcg gcaacggcag attttccagc gtgccgtgag
    79741 catcgctaaa cgcccagtca tgatcggtga cggaatagtt ccactcaacg ccccgacgtt
    79801 gtaacagtgc acctttttcc tgcgccacat cagcaatggt agaaggcatt tccggctcac
    79861 cgacaattgc cggtttttcg ctgcggaaga tgcctgcttt ctcgcgacca atactttcgc
    79921 gatctggacc cagccagtcg gtatgatcca gcgcaatact ggttactacc gcggcatcgg
    79981 catcgacaat attggttgcg tccagacgac cgcccagccc tacttccaga atcaccacgt
    80041 caagttgtgc ctgcttgaac agccacaacg ccgacagcgt accgtactcg aaataggtca
    80101 gggaaatatc accgcgtgcc gattcaatct ccgcaaaaga ggcggtgtgg gccgattccg
    80161 gcaattcccg gccctgcaca cgtacgcgct cggtataacg caccagatga ggcgaactgt
    80221 agacgcccac tttgtaccct gccgccatca gaatcgactc cagcgtacgg caggtggtgc
    80281 ctttgccatt cgtacccgca acggtaaaca caaatggcgc tggtttcagg acgccaagac
    80341 gcgccgcgac ctggctcacg cgctcaaggc cgagctcgat agttttactg tgcaggtttt
    80401 ccagataaga aagccacgaa gccagaggcg acgcggcttg aggagtgcgt ttgataatca
    80461 tggtatccgc tgattcgtta cggtgagaat agcaaaaggg cagagccagt ggccctgccc
    80521 ttatcagtta tcaggcctca ggttcctgat ccggtaccgg gggtaccact acgccttcac
    80581 gcggcgcttc aggattcggc gctggcagat tcatcaactt cgccagaatg ctcgccagtt
    80641 tcaggcgcat ttccggacga cggacgatca tgtcgatcgc gcctttctcg atcaggaatt
    80701 cactgcgctg gaatctaggc ggcagttttt cgcgaacggt ctgttcgata acacgcggac
    80761 cggcaaagcc gattaacgct ttaggttcag cgatgttgag atcgcccagc atggcgaaac
    80821 ttgcagaaac accgcccatc gtcgggtcgg tcagcacgga gatgtacggc aagccgcgct
    80881 cctgcatttt tgccagtgcc gcagaggttt tcgccatctg catcagcgac atcagtgctt
    80941 cctgcatacg tgcgccacca gaggcggaga agcagatcag cgggcagtta tcttcctgcg
    81001 cctgctcaac ggcacgcacg aaacgtgcac ccacaacaga ccccattgaa ccgcccataa
    81061 aggcgaactc gaatgccgca gcgacaaccg gcattccata cagagtgcct ttcatcacca
    81121 ccagcgcatc tttttcgccg gtttctttct gcgcagatgc cagacggtct ttatacttct
    81181 tggagtcacg aaacttcagc tcatctttcg gctcaagctc gctacccagc tccacaaggc
    81241 ttccttcatc taacaggcta tgcaggcgat tacgcgctgt catacgcatg tgatggtcac
    81301 acttcggaca gacctcaaga ttacgttcca gctcagcgcg gtataaaacc tgaccgcagc
    81361 tatcacactt agtccacacc ccttcaggaa tgctcgcctt gcgggtggga gtaatgttgc
    81421 ttttaattcg ttcaatccag ctcattaggg acctttctgt ctgaacttgg ttcgatgcca
    81481 gttttatctt tggggacgca taatgccatt tttgccccca acagaccatg aatgttgcac
    81541 attaaaacat aacagcccga aactttggat aaaaaagtgg tcgaaccgcg gagttacttt
    81601 ttattttgcg gcgcgtgccg cagcgcgttt gtgacggatt atttcgatga cgcccggcaa
    81661 aatagaaacc acaataatcc cgacgatcag cagcttaagg ttatcctgaa ccatcgggat
    81721 tgtaccgaag aaatagcctg cgtaggtaaa aagcagtacc cacaacagtg cgccgatcac
    81781 gttataagca gcgaaatgac ggtacgacat gtggcccatt cccgcaacaa acggggcgaa
    81841 cgttctgacg atcggcacaa aacgggcgag aataatcgtt ttgccgccat gtttctcata
    81901 aaactgatgg gttttgtcga gataactgcg acggaaaatt ttcgaattgg gattactgaa
    81961 taacttttca ccgaacaacc gcccgatagt gtaattgacc gcgtcaccca caatcgcggc
    82021 gatcagcatc agcactacca tcatatggac gttgagatcg ttggtttcca gtgatgccaa
    82081 cgctccggca acaaacagca atgaatcacc cggtaaaaac ggcattacca ccagaccggt
    82141 ttcacagaac aaaattaaga acaaaatggc ataaacccag acgccgtact ccgcgactag
    82201 ctctgccaga tgcacgtcaa tgtgcaggat aaaatcgatg aggaaataaa tcaggtccat
    82261 atttgcctat gccttgtact cgtcatcttt caggctgtaa ctacgttggc tacagcctga
    82321 aatgttccga gcattattct cgttagtccg ccagaaatag cgggcccatt ggcggttttg
    82381 gaagatcata ccggtcaggg taatccaccg cgaccagata cagcccttcc gcttttgccg
    82441 ttgctgccgc cagcgttctg tcctttgccg ccagcaactc tgctatccag ctctccggct
    82501 ggttgtgggc accgacttcc atcaggctgc cgacaatatt cctgaccata tgatgtacaa
    82561 aggcattcgc tttgatatca accaccacat aaggaccgtg acgcgtgaca ttaatatgca
    82621 taacgttgcg ccacggggtt cgggactggc actgcaccgc acggaacgag gtgaaatcat
    82681 tctcgcccag caagcattgc gcagcccgat gcatccgttc agcgtccagc ggttcgtaaa
    82741 aatgggttac ccctttactc agtaccgccg ggcgcagccg atgattgtag atgatgtagc
    82801 gataacggcg agccgtggcg ctaaatcggg catgaaaatc atcaggtaca gctttaaccc
    82861 aacgcacggc gatgtcacca ggtaaattcg catttacgcc caaggtccac gccgcgtctt
    82921 tgcgctgcgc ggttgtttcg aaatgcacca cttgcccggt accgtgtacc cctgcgtcag
    82981 tacgcccggc gcagaagacg gtgatgggtt cgttcgccac ctgggagagc gccttttcca
    83041 gcttctcctg cacactgcgg acttcattct gccgttgcca gccgtaatac ttactgccgt
    83101 cgtactcaat gcccagcgca attttataaa ctggcagttg ttgctggtcg gacattagta
    83161 cagatactcc tgcaccagtt tctcggcgat ttttactgcc atcagcgcgc cgccaaagcg
    83221 aacgttatcg gccaccgacc agagctggac ttgctccggc ataccgtagt cattacgcac
    83281 gcagccaaca gaaagatgcg gcgtacccga agcatcccct acctgagtcg ggaattcgtt
    83341 ctcttcagag agcacaatat cttcgccctg agcaaacgca tcacgcgctt cttctgccgc
    83401 cagcggacgc agtgcttcaa agttgaccat ctgggcatga ccgtagaata ccggtgcctg
    83461 gacgacgctt gccgaaatca tcagcccttc gtcctgcagg attttgcgta cttcgtcaac
    83521 gatacgacgt tcttcacgca cgctaccttc gctatccggc aacaacggca gcatattgaa
    83581 cgccagttga cgaccgaaga aatcttcttc gtcaatcgga atgccgttga gcaatttcgc
    83641 actctgcccc gctaacgcat cgaccgcttt tttgccctgg gcggaggctg aaatcaggct
    83701 ggtaacactg atacgcgaca aaccgccctg atcgattaac ggtttcagtg ccgccagcag
    83761 ctggctggtc agactgtctg gtacggcgat gacattccgg ttgcggtaat cggtcagtac
    83821 aaacgggttt acttccggca ccaccagcgg tacgtcgggt tcgagagcaa acagtccgct
    83881 gctgtcgatc accaggcaac ctgagttggt cgcttcttca acccaggcag cggtagcttc
    83941 tttgcctgcg acaaaaaatg ccagctgcgc ctgcgtccag tcgaattcag cggcatcctg
    84001 cacggtgatt gttttaccac caaagcgcag ttgttcgcct gcgctttcgt tacgtgccag
    84061 tgcataaatt tccccaaccg ggaactgacg ttcagccagc gtttcaagca gggcttcgcc
    84121 catagcgcca gttgcgccca ggacggcaat gttccagcct tcagacatgg tggtttactc
    84181 cagaaatagc aaagctccct gccaatgtta cagcagagag catgaagaag agattaacgt
    84241 gccggatgat gaacggcgtt aaaacccagt ttacacagca atgatgccgc actggcgtca
    84301 tcacaaatta catacagaga tgaccattca cggcgctcaa gatagttttt gcgcagttta
    84361 tcgaactcac ccggtatccc ggcgacttta cgcagcggtg cgtcatcgcg gcgcacatca
    84421 tacaccaaat gcaccagcct tttcagcgtc ggttgatcga gcggaccatg cagcgtaatg
    84481 cggccaaact caggcgcggg cagcaacgta tccagcgcaa cgtgttgttc atgcccaata
    84541 aacttgctat aggcttcaaa cacttgcgtc gtgccacgcg ctttaccctc caaggtatag
    84601 cctgcgatat gcggcgtgcc gatatccact tttgtcagca attccacatt aagttccggc
    84661 tcgccttccc agacatccag caccacgctt aacttctggc cttcattcag gcaggtcagc
    84721 agcgcggtat tatcgacgac tgcgccacgg caggcgttaa tcagaatcgc tccgggcttc
    84781 aggctacgga tcagtttttc atccgccaga tgtagcgttt tgtacggacc atctttaaag
    84841 agtggcgtat ggaaagtcag aatatccgcg cgctggacta actcatccag cgagcggaaa
    84901 tcaccctcat ccccacggtc ggcgcgaggc ggatcgcaaa gtaaggtttt aatccctaac
    84961 gcttccagtc gcgcctgcaa tcgacgtcca acgttaccaa cgcccacgat ccccaccgta
    85021 cggtcgtgca gtgaaaatcc atcgcgttcg gcaagcatca gcagggagga gaaaacatat
    85081 tccaccaccg caatcgcatt acagccaggt gcagcggaaa aaccaattcc cgcctgcttc
    85141 aaccatgctt catcgacatg gtcggtcccc gcagtggcag ttccaacaaa tttaatgggt
    85201 tttcctgcca gcaaagattc attcactttc gtgaccgaac gcaccatcag cgcatccgcg
    85261 tctgccagtt gagcgacggg gattggacgc ccgggaaccg cgatcacctc acccaaacgg
    85321 ctaaataagt cgcgggcata aggcatattt tcatcaacaa ggattttcac gtttgtgtta
    85381 cctgtatgag acgagagtta accggacaag tgtgccataa tctcgcggcc aggcatactt
    85441 gcgaagattt caggtataag gatacgtaat gatacaacct atttccggcc ctcctcttgg
    85501 gcagccacca ggtcagggag ataacctacc gtctggcgcg ggcaatcagc ctttatccag
    85561 tcagcaacgt acttcgctgg aaagcttaat gacgaaagtg acctcactga cgcaacaaca
    85621 aagagcagaa ctgtgggcgg gtatcaggca cgatattggt ctgtcgggag attcaccgct
    85681 gctttcgcgt cacttccctg ccgctgagca taatctggcg caacgtctgc tggccgcgca
    85741 aaaaagccat tctgcccgcc agcttttagc gcaattaggg gagtatttac gtctggggaa
    85801 taatcgtcag gcggtcacgg attatatccg tcataacttt ggtcagacgc cgctgaatca
    85861 gctctcaccg gagcaattaa aaaccattct caccctgttg caggaaggga agatggttat
    85921 tccgcaacca cagcagcgcg aggcgaccga ccgtccttta ttaccggcgg agcacaatgc
    85981 gctaaaacag ctggtgacca aacttgcggc ggcaacgggg gaacccagca aacagatctg
    86041 gcaatcgatg ctggaacttt ccggggtgaa agatggcgag ttaattccag cgaaactgtt
    86101 taaccatctg gtgacctggc tacaggcgcg tcagacgcta agccagcaaa atacgccgac
    86161 gctggaatca ctacagatgg cgctaaaaca acctttagat gccagtgaac tggcggcgtt
    86221 atcggcatat atccagcaaa aatatggcct ttctgcgcaa tcatcgcttt cttctgccca
    86281 ggccgaggat attcttaatc agctttatca acggcgggtt aaagggattg atccgcgtga
    86341 tatgcaaccg ctgcttaatc cttttccacc gatgatggac acgttgcaaa atatggcaac
    86401 gcgtcccgcg ctgtggatac tgttagtcgc gattatcctg atgctggtct ggctggttcg
    86461 ttaaccccga cgaaatgaca gtatcgtgac aataataccc agcaccgcag agatcgcccc
    86521 ggcaagaaat accgaagagt aaccaaacgt ggtcgccagc attcccgcca gcggcccgga
    86581 gacgccgagg gcgatatcct gaaacgcggc gtaaccgccc agtgcggtgc cgcgaacttg
    86641 tgaggggacg cgtttaacca cctccacgcc cagcgcagga aagataagcg aacatccggc
    86701 tccggttaac gccgcgcccg ctaatgcgac ccatgcacct ggggcttgcc agagcagcaa
    86761 caagcccacc gtttctacaa gcagagagac aatcgccact ttcacgccgc caaaacggtc
    86821 cggcatccag ccaaacatga cgcgcatcac gacaaatgcg ccgccaaacg cggtaagagt
    86881 aaagcccgcc atcgcccatc ctttgctggc aaagcagagc gaaacgaaag tcccgataac
    86941 cgcaaaacca acgccttgta gtgccagccc taaccctggt ttccagataa gcccgacaac
    87001 gctccacagc gatggacgtt ctcccgccag ggccggtact ttgcgcactg tgccgttaca
    87061 ggcccacgcc agtaagggta ataccattgt ggtgatcgcc agtgcggcaa aaccgtaatg
    87121 gctatgaatc aacaggccaa gcggcgcacc aacagcgagg gcaccgtaaa tcgccattcc
    87181 gttccatgac atcactttgc cagagtgttt tggccctacg atgcctaacc cccaggtcag
    87241 agcgcctgtc agtaactggc tttcaccaaa cccaagaatc aaacgcccga tgaccaacag
    87301 ggcaaatttg aacggtgcgg agacaggcaa aatcgccgcc agcagcaacg cgccgccagc
    87361 cagaccacaa gctaacatcc cctgaagcgc cgaacgtttt gcgccatatt gatcggccag
    87421 tcgcccggcg taaccacgcg tcagcaccgt agccagaaac tgaatcccga cggcaatgcc
    87481 gaccatggta ttgccatagc ccagatcatg atgaacaaac agcgggataa ccggcaacgg
    87541 caaccctacg gtcatgtagg tgagaaaaac cgcaaaagcg atgcggaaga gcgaaaaact
    87601 ggcagaagat cgtgtttcgg tttggcttac agcagtcatg cattactcca gaatgcagcg
    87661 caaggcgagg agtatccccg tctcatctct ctggtttcag ggttacggtg cgttggcagg
    87721 atttaacgcg tacgtctttt cagaaggaaa tcgacaaagc gggaagtttg cctagaactg
    87781 gtggcgattg tcaatgatgt gaaaaaggga accatcaggt tcccttttgc gttagtgccg
    87841 gaggccgcat tggcgcgtaa cgtcggatgc gacgctggcg cgtcttatcc gacctactcc
    87901 ggcctactgc gaattaatct ttcagcttgc gcattaccag cgtggcgttg gtgccgccga
    87961 agccgaagct gttagacata acagtggtca gttcgcgatc ggtcgtttcg gtcacgatgt
    88021 tcagacccgc agcctgctcg tccagctctt caatgttgat gctcggggcg ataaagccgt
    88081 gttccagcat cagcagagag tagatagctt cctgcacacc cgcagcgccc agtgagtgac
    88141 cggtcatggc tttggttgca gaaatcgccg ggctcttatc gccgaacact tcacggatag
    88201 ctgccagctc tttcacgtcg ccaaccggag tcgaagtacc gtgagagttc aggtaatcga
    88261 ttggagtatc aacgccatgc atcgccatct gcatacaacg tactgcgcct tcgccagacg
    88321 gagcaaccat gtctgcacca tcagaggttg cgccgtagcc aacgatttca gcatagatgt
    88381 aagcaccacg cgccagcgcg tgttccagct cttcaaccac taccataccg ccgccgccag
    88441 cgataacgaa accgtcacgg tgagcgtcgt aagtacggga ggctttttcc ggggtgtcgt
    88501 tgtatttagt agacagcgca cccatcgcgt cgaattcgca ggccatttcc cagcacagct
    88561 cttcgccgcc gccagcaaac acgatgtcct gtttgcccag ttggatctgc tctactgcgt
    88621 taccgataca gtgtgcggaa gtcgcacacg cggagctgat ggagtagtta acgccatgaa
    88681 ttttaaacgg cgtggcgaga caggcagaaa cgccggatgc catcgcttta gtgaccacat
    88741 acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc gtcagcgccg aacacctgga
    88801 aacgcgggga accgccgccg gaacctgcaa tcaggccaac gcgcgggtta ttctggtaag
    88861 cttccggaga gaggcccgca tcagcgattg cctgctccat agaaaggaat gcataaatgg
    88921 atgcgtcgct cataaagcgc acaactttgc ggtcaatgag gccagtggta tccagtttta
    88981 tgttgcccca gacgtggcta cgcatgccgg aatccttcag ctcctgagag aaagtgatcc
    89041 ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg gttattaccg atgctggaaa
    89101 caatgcccag gccagtaatc actgcacgtt tcattcaata cctctgtaag tcgcacatag
    89161 agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg aacaagtccg atcagccatt
    89221 taatagagaa atttgcgcag ccttacacac atcgctaaga tcgagccacc gcctgtaaga
    89281 cgagtaactt acgtgaaaca ctactccata caacctgcca acctcgaatt taatgctgag
    89341 ggtacacctg tttcccgaga ttttgacgat gtctattttt ccaacgataa cgggctggaa
    89401 gagacgcgtt atgtttttct gggaggtaac caattagagg tacgctttcc tgagcatcca
    89461 catcctctgt ttgtggtagc agagagcggc ttcggcaccg gattaaactt cctgacgcta
    89521 tggcaggcat ttgatcagtt tcgcgaagcg catccgcaag cgcaattaca acgcttacat
    89581 ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt tagccttagc gcatcaacac
    89641 tggccggaac tggctccgtg ggcagaacaa cttcaggcgc agtggccaat gcccttgccc
    89701 ggttgccatc gtttattgct cgatgaaggc cgcgtgacgc tggatttatg gtttggcgat
    89761 attaacgaac tgaccagcca actggacgat tcgctaaatc aaaaagtaga tgcctggttt
    89821 ctggacggct ttgcgccagc taaaaacccg gatatgtgga cgcaaaatct gtttaacgcc
    89881 atggcaaggt tggcgcgtcc aggcggcacg ctggcgacat ttacgtctgc cggttttgtc
    89941 cgccgcggtt tgcaggaagc cggattcacg atgcaaaaac gtaagggctt tgggcgcaaa
    90001 cgggaaatgc tttgtggggt gatggaacag acattaccac tcccctgctc cacgccgtgg
    90061 tttaatcgta cgggcagcag caaacgggaa gtagcgatta tcggcggtgg tattgccagc
    90121 gcgttgttgt cgctggcgtt attacggcgc ggctggcagg tagcgcttta ttgcgcggat
    90181 gaagcccccg cgctgggtgc ttccggcaat cgccaggggg cgctgtatcc gttattaagc
    90241 aaacacgatg aggcgctaaa ccgctttttc tctaatgcgt ttacttttgc tcgtcggttt
    90301 tacgaccaat tacccgttaa atttgatcat gactggtgcg gcgtcacgca gttaggctgg
    90361 gatgagaaaa gccagcataa aatcgcacag atgttgtcaa tggatttacc cgcagaactg
    90421 gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg gcgttgcgac aaattgcagc
    90481 ggcattactt atccgcaagg tggttggctg tgcccagcag aactgacccg taatgtgctg
    90541 gaactggcgc aacagcaggg tttgcagatt tattatcaat atcagttaca gaatttatcc
    90601 cgtaaggatg actgttggtt gttgaatttt gcaggagatc agcaagcaac acacagcgta
    90661 ctggtactgg cgaacgggca tcaaatcagc cgtttcagcc aaacgtcgac tctcccggtg
    90721 tattcggttg ccgggcaggt cagccatatt ccgacaacgc cggaactggc agagctgaag
    90781 caggtgctgt gctatgacgg ttatctcacg ccacaaaatc cggcgaatca acatcattgt
    90841 attggtgcca gttatcatcg cggcagcgaa gatacggcgt acagtgagga cgatcagcag
    90901 cagaatcgcc agcggttgat tgattgtttc ccgcaggcac agtgggcaaa agaggttgat
    90961 gtcagtgata aagaggcgcg ctgcggtgtg cgttgtgcca cccgcgatca tctgccaatg
    91021 gtaggcaatg ttcccgatta tgaggcaaca ctcgtggaat atgcgtcgtt ggcggagcaa
    91081 aaagatgagg cggtaagcgc gccggttttt gacgatctct ttatgtttgc ggctttaggt
    91141 tctcgcggtt tgtgttctgc cccgctgtgt gccgagattc tggcggcgca gatgagcgac
    91201 gaaccgattc cgatggatgc cagtacgctg gcggcgttaa acccgaatcg gttatgggtg
    91261 cggaaattgt tgaagggtaa agcggttaag gcgcggtaat ctgctctcgc agtgtttgcc
    91321 ggatgcggcg tgaacgcctt atccaaccta cgtgtgacgt gtaggcctga taagacgcga
    91381 taagcgtcgc atcaggcatg gtgctccaga cgccgcaact tacagctgtg acgcctgctg
    91441 aaacaggttt tcccacatat cggtcaccag cgcctggtca cgcggcgaca attcaccggc
    91501 accaatggct ttttccagac tctggctaac ggtcgtatgc accgcctgag cggagtggtc
    91561 atcaccactt tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat acccactggc
    91621 aaacaactca tcatcactgg catggtcaac catgccgtcg attaatgcca gaatgcgtga
    91681 ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg tggcgctggc ttcagttgag
    91741 cgcttccggc cacgggaact gttccgccgt aagttccggc gtgtgataat aattttgtag
    91801 tgctttaatg aagcgtgccg ggcgttccgg aatgccattt gtcagataat ccatcacctg
    91861 cgcatgtacc cgccgttgaa acaccacacg atccggttcg aaatcccctt ccagattgtc
    91921 gcaactgacg ttaaacggat atccagccgc cacgcagaat aaccagtcga acgcctgcgg
    91981 cttcacttca acatcttcaa actggctttg cgtctgggca tcgcgtccgt ccgggcaata
    92041 ccagtagccg aaatcaacca gctcacggcg cgcttttccg gcaatacacc agtgcgaaat
    92101 ctcatgaatt gcgctggcat aaaagccatg agcaaagacg attcggttat acggtacttc
    92161 cgcatcagca ggaagataga tcggttcgtc gtcgccttta atcagacggg tattaaattc
    92221 atcggcaaag cagctattaa aaatttcaat caactgctcg tagtggtgtg tactgttcat
    92281 tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc tatcgtaaag taatttggca
    92341 ctcatcaccg ccgagacgat aacaatcatc gggcggatca gcttttgtcc tttgctcaac
    92401 accaaccgtg aacccatgcg cgcgccgagg aactgcccca ccagcatgac aaagcccgtc
    92461 gcccaaatca ctttgccgcc gagaataaac agcagcagac cgccaatgtt tgacgttgcg
    92521 ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga atccgcacag cataacgaag
    92581 gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc cgtcataaaa accgacgcag
    92641 ccaccggcaa ttaacgcaaa gggtaaaccg tacatccggc gctggcgatc ttcttcaccc
    92701 agctttggca tcagcaagaa atagaggccg atacaaatca ccagaatggg caaaatctgc
    92761 cgtaagacat cagcctgaac gtactgtacc aacagtgcgc cgctcattga gccgacaaag
    92821 gtcatggcaa tattgagttt ctgatcgctt aaactaacca ctttgcggcg aataaagtag
    92881 atagtggcgg aaatagagcc gccgcaggct tgcagtttat tggttgccag cgcgttagcg
    92941 ggagacattc ccgcagccat caatgccgga atggtgagta acccaccgcc accggcaatc
    93001 gagtcgataa atcccgccag catggcgaca aaaaagagaa ctcccagcaa cagcggagaa
    93061 accataaaca ggctattaaa cgtttccatt agatcacgtg ctcatccagt agcgcctggc
    93121 aggaaggcgg caacggaggc ggtgtcttct tctcaggctt tgttgttccc ggttttggag
    93181 gttcaaacca gctttgcagt tctgccccgc aaccatcgcc tgatggcggt aaaggttgat
    93241 cttcacactc cagactatcg gcagggcaac gtaatcgtac atgcatatgc gcgcgatgct
    93301 ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca aggcaaagtt
    93361 gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc tttgtcctgg gcggcgagtt
    93421 tgatcaagct gaaaatttct ggcttccaca gcgtggagac aacgtgttta ccgtcgcggg
    93481 aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga gttttcggca
    93541 gttgcagaaa gatatcgaca tccagtccgg tctggtggct ggcatgaccg ccgttgaaac
    93601 gcccaccagc gggcatcccc atatcgccaa tcagcaccgt acccatgccc agattgctca
    93661 cctggctact cagacgctgg ataaacatca ccagatccgg gtgaccgaaa tagcgacgct
    93721 gatcggtacg catgacctga taatgttcgg actgtatcgg caacgtgtca gcgccgacaa
    93781 tacagccatt agaaaaactg cctatcgatt gtgcgctacc cggcacaggt tgggttattt
    93841 tttgccacgg cgtcgctgcc aggctagcgc tactggcaag cagagccagc agcgcaatcg
    93901 cggttttgtt cattttttac cagcgtggaa tatcagtctt cacatcggca ttttgcgccc
    93961 gctgccgtaa caggtgatcc attaaaacga tcgccagcat cgcttcagcg atcggcactg
    94021 cacggatccc gacacaggga tcgtgacggc ctttggtgat catctcaact tcttcgccaa
    94081 agcggttaat agtgcgcccc ggcacggtaa tgctggaggt cggtttcagc gccatatgag
    94141 caatgatttg ctgcccgctg ctgataccgc cgaaaatgcc gcccgcatgg ttgctctgga
    94201 aaccctcttt ggtgatttcg tcgcggttct ggctgccgcg cagcgccact acgtcaaaac
    94261 cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg gcgatgtcgg
    94321 catccaggcg gtcaaagacc ggctcgccaa gtccggcggg gacaccactg gcaacaacgg
    94381 tgactttcgc gccgatggag tcgccctctt ttttcagcgc gcgcatcagt tcatctaacg
    94441 cgtcgatttt gtcggggtcc gggcagaaga acggattttg ttcaaccagc gaccagtctt
    94501 tgatttccag cggaatgtcg cccatctggg tcaggcagcc gcggatttca ataccaaatt
    94561 tctcggcgag atattttttg gcaatcgccc ctgccgccac gcgcatggcg gtttcgcggg
    94621 cggaagaacg tccaccgccg cgataatcgc gcagaccgta tttttgttcg taggtgtaat
    94681 cagcatggcc tggacggaag acgtctttaa ttgcgctgta atcctgagaa cgctggtcgg
    94741 tattttcgat caacaagcca atgctggtgc cagtagtaac gccttcaaaa acaccggaga
    94801 gtattttgac ctgatccggc tcgcggcgct gggtggtata gcgcgatgtc ccagggcgac
    94861 gacgatcaag gtcatgttgc aggtccgctt ccgtcagcgg aatgcctggc ggaacaccat
    94921 cgacgatgca gccgagcgcc agcccgtgcg attcgccgaa ggtggttacg cgaaagagtt
    94981 gtccaattgt gtttccagcc atcacggctc cgttattgtt gtgtttgcgt gtttacttaa
    95041 tctttataaa tcgcgaaatg ttctcgtgcg gcaagaagct gctctttagt gagcataaac
    95101 acaccatcgc cgccgttatc aaactccagc caggtgaacg gaacatcggg atattgttcc
    95161 ataagatgta ccatgctgtt gcccacttca caaatcaaca cgccatcatc agcaaggtaa
    95221 tctgccgcgt taccgagaat gcgacgcgtc agtttcaggc cgtcggtgcc agatgccagg
    95281 cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc cgcgtcgaca
    95341 tacggcgggt tagtgacaat cagatcgtat tgcactttcg gcaaatcacg gaacagatcg
    95401 gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt cgatgttctg ttcggcaacc
    95461 gccagcgcgt cgggagagat atctaccgcg tcgacttccg cttccgggaa ggcataagca
    95521 caggcaatgg cgatgcagcc gctaccggta cacatatcta aaatatgctg cggttgcttg
    95581 ctgatgagtc cggcaaattt attgttgatc agttcaccaa tcggcgagcg cggcaccagc
    95641 acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg ctttgttggt caggtaagcc
    95701 accggaatgc gctcgttgac gcggcggatc acgcgttcaa caatacggtg tttttcgctg
    95761 gaggtcagac gcgcggtgcg catatcttcc gggatatcca gcggcaggta gagcgatggt
    95821 aacaccagct gtacggcttc atcccacggg ttatcggtgc cgtgaccgta ccagatattt
    95881 gccgcgctga agcggcttac cgaccagcgc aacatgtcct gaatggtttg cagctcattt
    95941 actgcttcat caacgaaaat tttatccacg tattcctcca gggcatgatc gcaataattt
    96001 cggcggctag tttgccatga agatgacgat aaatcagcat tcacgcgcgg tgagtgagga
    96061 aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt aaactgtagg aaaattagaa
    96121 atagagacag ataaatgaaa aagaaaacaa cacttagcga ggaggaccag gctctgtttc
    96181 gccagttgat ggcggggact cgcaagatta agcaggacac gattgtccac cgaccgcagc
    96241 gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct gatgccagcc
    96301 attatttctc cgatgagttt cagccgttat taaataccga aggtccggtg aaatatgttc
    96361 gcccggatgt cagccatttt gaggcgaaga aactgcgccg cggcgattat tcaccggaat
    96421 tgtttttgga tttacacggt ctgacgcagc tgcaggccaa gcaggaactg ggcgcgttga
    96481 ttgccgcctg tcgccgcgaa catgtgtttt gtgcctgcgt gatgcatggg cacgggaagc
    96541 atattttgaa gcaacaaaca ccgctgtggc tggcacaaca tccgcatgtg atggcatttc
    96601 accaggcacc gaaagagtac ggcggtgatg cggctttgtt ggtgttgatt gaagtggaag
    96661 agtggttgcc gccggagttg ccctgaatgc tggcagcaaa tgccggagca gcgtaaacgc
    96721 cttatccggc ctacaaatat gtgataattc aataaattgc aattcacttc gtaggcctga
    96781 taagacgcat cagagcaata ctacgaactt cagttcattc aatacatact gaaaacgaca
    96841 cgtcgaatca atcggttgcc cactactggt attcacattc accacaatat cgtgtagtac
    96901 tttatccgtt accggatgac gagttggcgt aaccgttacc gttgatttat cacaaccgta
    96961 agaggtaaag gttaacaatt ctgataccgc gcggcagtat gctcctctgc catcattatc
    97021 agaaaaatta ctgccatcac agagtctgcc gccttccatc aggtaggtcg aaagcgtggt
    97081 ggtcactgtt gaccccgtgg actgaattcg taacgcctta ttatggctga cataattttc
    97141 actccaggag atatcgtatg aagcgaccgg tatcgttggg catattgtgt tatgcactgt
    97201 aggggccgta tagcttccaa atccgggtaa atctgtatag gaataaaagg atgcgatcca
    97261 agggcaacca taatcatcag atgcaacggt aattttaaac gtcatatatg cacaacgaaa
    97321 aattttcgta tctatgccag ggcaatttga gttattaatt gtatcaaagt cagtcagtac
    97381 caaagttgga gctgaacccg acccagacca ggaaataacc cgcaacgtat cgcctttata
    97441 cgcaaaagaa tcatatttcc catatgcaac ctcctttggc gttatcaccg taacattgaa
    97501 aacactgctc gctacagcac cggttacctt gtccatggca taataaactc tcacactgtc
    97561 aaaactcgca ctcataagtt tatctccagc ataactctta gatgcgccga caacactcag
    97621 aacgagcagc attaggctcg cgaaaattat ggtccatttt ttcatttcag aaatactcaa
    97681 aggttgccgt cactgtggca ctaaagtctc cggacgttac atctcgcccg ctctttgcct
    97741 gtaactaggt attaaaattt aggctgttat ttccctgatt cagcgggagc gtgacgcctg
    97801 ttgtattgtt gattggcacc gatgtgcctg ccgcagtttc aataccaatc ccagcgccgg
    97861 atgctgatga cgaggagtca aaggccagaa agccgggtag agcactatct tctgttcctg
    97921 tcaacgttac tttgacatta aacaatgtag aactatgaca atctttcaat tgaaacacca
    97981 atggcactcg ctctgactgc ccaaggttca ttaaatcgct ggcggcaatg gcaggaaaat
    98041 gtacttctgc caactggcta ccattaatca ctggcgtaca cgatttactg attaaattgc
    98101 cggtaaagtg caaattatct cctgctattg tggttccact gcccagcaac atcaggctac
    98161 taataaaaaa caacgtacgc tttttcttca ttggtaatct acctgtaacg tggcccaggc
    98221 ctcaaagtcc gcctctggca gaatgacacc actctttttt actggcacag ccgttaacac
    98281 cggtaaatcg gtttcattca ccacaagtgg cgtattgatc gtaaaactct gccctgcctg
    98341 tttgagctga atgcctaaac cagtgatatt gctgcttact gcagcattat caaaatcgct
    98401 tggtgtgcca ctccagctca aggtcatttc cattaatggg tcgcgaaccg ccgtatcgca
    98461 ggtaatggaa tacggcacgg tttgctcata attgctgcca ttaatgtcat caataagcac
    98521 atcctgaaaa gttatttcaa tggtttgagc gttgctaatc gtgcagttag gcggctgcac
    98581 taaataaccg tgaaaagaga cgtttgagag tgccatcgag caaaagcccc acagcaaaat
    98641 aagcgagatc cgtttcattg gtaatccacc acaattgtgg cactggcgtt aaattcagct
    98701 gccgctaacg ttgtaccact ctgtttcacc ggcacagctt ccagaacggg aactcccgag
    98761 gaaaaattaa atggcaacca tgagccagag gttaagtcga gtattgtgtg atcactactt
    98821 ttctgaaccc gaataccaaa cccaggaata cccgtgctga taactgtttc accgttaatc
    98881 acgaccgttg tcgcctgcat ctgtagacgc aggtcatcca ttgccagatt gttacaaaca
    98941 agcgagtagt caatgggacg tttataatca accctattga tcttattaat gaatacatta
    99001 ccaaactcaa ctgccgcacc atttaccgta caaggaggag gggcatcgac aagcacccgt
    99061 aaattcatac ttactgactt actcgctgca actgcaaagc cgctaccaac acaacatatc
    99121 agtaacccag ttaccgccca gacagaacaa gatcctttca tactggctct cccggcctca
    99181 gcctttgcgg ctctgctctt catcgaccta gcaactgtta ccgttgcaag tgaaaaagag
    99241 cggcatccga gcgccatagt cgttgacata ggtcagtacc ggcacgctac ccatcttcac
    99301 attcagcggt gcagaggttt tcggcgacat cactattggc gagaaaccgg ctgccggatt
    99361 gccatttttc tgtgtgctgg cattactgaa aatgacgtaa tacgacgtcg ggttattgac
    99421 ggtaaattcc tgaccgttgc gggttaaagt aaccttatat tgataggggt ttttcatgct
    99481 gacgttctcc agcgccttcg gtcgccagaa caacttaata cgcgtttgta gtgctatttg
    99541 cagcgtgttg gctttgtttg attttggcgg gatctcacgc acgttgaagt agaacaggct
    99601 ttcacgatcg gcaggcaatt tgttgatatc cggcattccc tgcactttta cctgcccgtt
    99661 catcattgaa tcgatgcgtt gcaccggtgg cagaacagtt aacggtgaac tgatcttgtt
    99721 acccttttca tcttcgatcc agctttgggc cagataaggc agtttcggat cgttattgcg
    99781 caacgtcacg ctgattgatt tgtcgctttc attaaatatc aaacgggtac gatctggcgt
    99841 tacagaagcc tgggcagaca aactggcagc gctaagcagt aacgctagcg tcgtagcacc
    99901 caatttggca gagcaaagta aatcagacat ggatcattcc ttaatcacgt tattggttag
    99961 ctgaaacaga aacaggggga ttggtgggtg tcacctgttg cgtctgctcc tgaatgacag
   100021 gctttatgtc atcaggaaca accggcgcga cgttgccttt atgctggcat ggcagtaaca
   100081 agccgttaaa cagatcggca ggcagcgggt ccggcaggtt gatatcgcaa tgcgcaacac
   100141 cactccagaa cacactcatg tgttcgccag gtttcacccc agccagataa acattgccgt
   100201 catcatcgac aaggcccact gtctgctcgt tatcattttt tacttctgcg ccaaacggag
   100261 gatggctgcc gtcttgtaaa cgcagcaccg ccatcgcttt ttgaccactg atgacggcaa
   100321 atttgcggta gccaatcgcc ccttcagtta gcgtggcttg taccaccgac tgggttgctt
   100381 cggcgttttc cggcagtttg ttgaggtcga tatacgcctg attgcggtaa tagttattga
   100441 cgtcagaaac gacggcttta ccaaacatat tggtataaac agcagcaccg ttaccttcta
   100501 ccggaacatc ggcaacgcca tcggcatcaa tcaacaagcg tgtcccgccc atattctggg
   100561 tacggtgaag tgcgccaccg tgggcagtaa acgttgcgcc gccctgtaac gacaagcccg
   100621 cagaggtgta ttgcccttca tggtagttcg cactgaggtc aacctgcgcc agcgaaccat
   100681 catggctgta atagccgtca acgctggtgt gtttgtcact ggtgccgacg ttcaactgat
   100741 agtgagtcgc gtcatcgaca cggctgaaat aaccgacctg actgctgtcc gtcccactgc
   100801 catagtaccg ttatagctaa cggtactgtt gtcgccccac ggcatactga gcgaaatgta
   100861 catgcctttg tcggcctggt tgtcatactc gtaacggtag ccagtcatcg agatgctgac
   100921 attacgaata ctgcccatat tgaaatagtg cgagagcatg atgttgtagt ttgtctgctc
   100981 ctcgcgatcc cagtaggtat ggcgggtata gttgagataa accgaaacac ccgcatcgcg
   101041 gaagttctgg ttataagtgg cggtgtacat ctctttgtcg ttgcccgtgc gtaccattcc
   101101 gctgtcactg gcatccagat actcgctcat ggtcataaag ttctcttccg agaagcgata
   101161 tccagcaaaa gtaacgcggc tgttgagctg gtcaaagtct ttggaataac tcacacggaa
   101221 ggagttaccg tccagcgaac ctttgccata agcggtgtct ttatccagtt tggtgtgcga
   101281 gtgagtaaca tcaaacgcga ccgcgccgaa tgtagacaaa tcgcgaccga caccaagcgc
   101341 cgcagactga tagttttcat ctcccagtgc gccgccatat aacgaccagc cgttagcgat
   101401 cccccaggaa gcttcagcac cagaaaaaaa ttcaccctcg acatggtgtc cccactcttg
   101461 cggacggccc atcatgatct tatagcgaac ctgacctgga cgagtgaggt atggcatcga
   101521 ggcggtgctg atgtcatatt cctgcacctg gccgttctgt tcttcaatgc gaatatgcaa
   101581 cgtaccggag acagaatcac caagatcctg aatacgaaac ggtccagccg gaacctgcgt
   101641 ttcgtaaatc acacgcccca tctggctgac ggtcactttt gctgtggtgt gtgccacgcc
   101701 ggaaatgtct ggcgcgtagc cgcgcagatt gggaggcaac atttgatcgt cagtactgac
   101761 actgccacca acatagttaa aaccatcaaa aatatcggat ctgaggtaat cctcgcccag
   101821 cgccagtttg gcttttaatg atggtaacgc ccgcctggca tagtagcgac tccactccca
   101881 ttttttttga gtttcatcgc cgctgaattc atcgtcatca ttactgcgag tatgttgata
   101941 gttagtctgc cagtcagcac gcatacgcca cggccctagg ttaaccccaa ccgtcccgtt
   102001 gccgctgatc tcgttactat catcaccgcc attttcttcg tgccgggttt gtgcgttgat
   102061 gctgtagtcc gcgacgatcc cggagatgcc gtcatcccaa cgtgaaggcg gatcccagtc
   102121 gggataagta tattcgaggt aagcctgtgg cagtgaaatg actaatgcgg actggcttaa
   102181 atcagcctta atttccatgc cttccagttg accggatttc aggcatttag catcgtggct
   102241 ccattgcaga tttttcgcca cgtcttcttt taaaccaaac tgcgctacca gttccggtgt
   102301 cagacaagca tagcttttgc tcgcgtcatc ttcaccagca taccagtaaa tatcgtactc
   102361 ttccgccaat ggctgtttat ttagttgaac ctgtaaattg tattttccgg gctcaacata
   102421 tccctggctg gaaaaacgct tcagatcaat ttttgtgtcg ccttttaatt ccagaaaacg
   102481 ggaatcaaac tgaatgtcgt cttcagccca gacactgcta taactccctg acattgccag
   102541 cgcaatgcac cacggaagaa tccgtaatcg aaaaagagaa tggtcaggca tacacgtttc
   102601 tatccgtttt tattcttgtt cggtcaaaat ccatttaaat cccccatagc agtgtcgcta
   102661 tgggggtatg ccttacaggt aagtaatctg gaaggtggta tttgcttcaa aattacccag
   102721 atctggcgca tcagctgcgc cgaccagcca ggctttaaag ttcagtgtct gcttcgcttt
   102781 gcctttgttg gtagaagtgt ctttcactat tttctggtca atacccatgc cgcttttgta
   102841 agaggtaccc agagagtcac cgatcgccag gctaacattg ttaaatgccg caccggtatc
   102901 ggtattgaaa atggtgtaat aattgccgct atttgcagaa gaaacggtac cagtaaaggt
   102961 agtggtcatt gtttcctgag tatcaaatac gcaatcttgc agacgaatct ggaaactttt
   103021 cggcgtggtg gtgccgttat ttttcagttt ttcggcaccg atatcaccca tatcaacttc
   103081 cagtttgtga tcgtccggaa cgatagaaca cactgagtct tcaatggtgc cgtaaaaacg
   103141 tgcagtaccg ttgttacccg cagcgattgt tgccgtagag gtgaacacgc ccatcaccat
   103201 tgccgcagca atagctgttt taacaaactt actcattatt tgtccttaaa ctataaataa
   103261 ttaaaaatat aaacattgca atacattgat tcagtcaata gccaatgttt atacccagcc
   103321 attgaatacg gcatgcgacc caaaaagaac aggtcgtaga aaattgtatt cattggtgtg
   103381 gggccgcata gtgatgtttc cacacccctg atccaattta attttactaa agagtaaatg
   103441 tagatttaat taatatattg acagaagaaa ggttatctta aggacatcct taaaaaatac
   103501 ggaataagta tttagaaaaa atagaaagta tatcaataat gaatatcaac agcttaaaaa
   103561 acaaacaatg aacatataat gcgattggca tttatccact tttgacaata taatatataa
   103621 acactatagc cagaatttaa tcaagtttca attacatcct gcctgattat tcaactctat
   103681 tttctctatt atctgtatta ataaatgaca ctgagtgtca aacagacatt attcatttca
   103741 acatgtaact ttgggcaaaa tcaagatacg cgcagaagaa taatttccga atgaatgtag
   103801 aaagtgtttt atttatagat aaggagagtc ttcgatagta gtgaggttga cagatatccg
   103861 ctgcctaatg agcaattatg cccaatgaat ctacctcatt gggcataatt tggaacacat
   103921 cagatagctt ttgccatctt cagattacac gggctcatct gccagttgaa tgtaccgttt
   103981 ccgctttcat caagcgttac gctggcaatg gccgaggtgg taaacatagg cggcgtttcg
   104041 cccggacata actcggcaac cagatacccg actaacggta agtgagagat caccagtacc
   104101 gaagcaacac cttcattggt caacgcctgc aaataggcac tgaccagacc aacatcgccg
   104161 cagggcgtta actccggcag aacttctgca ctggaaggca ggttcagaca atcccctact
   104221 tcttccagtg tttgctcggc tcgcaggaac gggctcacca gaacgcgttc gatttccact
   104281 ttttgacctt tcagccagtt cgccatcagg cgagattcgt cacaaccatt agtggtcaga
   104341 ggacgaaccg aatcactggc ggcatcgagg gctgcgtcgc cgtgacgcat gataaaaact
   104401 tgcatattgc accgcttttg ttaaccagat tcgcccgttt tctttcatct gaaaccagag
   104461 aaagaaaacg gtggccggca ttgtgcctta tccattcacc gaatgaaacg ctgtttttta
   104521 cctcaatggc gtaagtatag tcaatatgcg tttacatttt gaccaacact ccgccattca
   104581 gcgcggattc atatagcttt gaccttctta ttgcaggtca gttgcagttg ttttccaaaa
   104641 actttcccca cgcgcgccca tctcgaccaa acgctcgcaa ggggtaaaac gggaaccata
   104701 ctgcgtggcc agtcgttgca ttattgcaac cacttcgccc gcgccgagag aatcgatata
   104761 gcggaacggt ccaccgagaa atggcggaaa accaatgcca aataccgcac caatatcccc
   104821 gtcacgcacg ctacggataa cctgctcatc aacacaacgt actgcttcat tcagcatcaa
   104881 catcacacac cgttcagcaa cctgcggtgc ggagagtcgc ccctgccctt gtgcgccaat
   104941 gagcgggtaa atggcaggat cgacctgttt tttgctttta cgccctttct gaccataaag
   105001 atagaaaccc cgaccatttt ttctgccttt gcgatcgtcg ttcaaaattg aagaaacaac
   105061 atttgcaggc gcgctaaaac gttctccata agcggcttcc agtacaggaa taattttagt
   105121 cccggtgtcg attcctacct catccaaaag ttggattggg cctaccggaa aaccaaattt
   105181 cactagcgcg gcatcaatgt gctctacccg ttcaccttcg gtcagcatgc ggatagcttc
   105241 attaatgtaa ggcgctaaga tgcgattgac gtaaaacccg gctttgtcac gcacgacaat
   105301 gggcgttttg ccctgttttt tcgccagttt taccgtagtg gcgatggttt gcgccgatgt
   105361 acccgcatga ggaataatct ctaccagcgg cattttttcc accggactga agaaatgcag
   105421 gccaataact tgctcaggtc gcgcggcgtg agcggcgata tcaccaatcg gtaaagatga
   105481 cgtgttcgaa gcaaaaatgg tatgagcggc gcaattttgc tccacttccg ccaccatctg
   105541 ttgttttaat tcgagatttt caaacaccgc ttcaataatc agatcgcgat gggcaaagcc
   105601 gcgatagtcc gtcgttccgg agattaatgc cagctgtttg tcacgttcgc tggctttgag
   105661 atgacgacgg cgaactttgc cctccagctg atcccaactg tacttcagcg catgatttat
   105721 gccctgcggg ttgatatctt taattctgac cggaagcccc gctttacaag cagtgacata
   105781 agcaataccg ccgcccatca agccaccacc taaaattccc acgctgttta atggcgcagg
   105841 cggtgcatca ctaccgggat ctttcttcac gtccgtactg gcaaaaaaga tactacgcaa
   105901 agcttgcgat tgcggcgtca tcgccagttc gccaaaggcg cgagcttcag cgtcatagcc
   105961 gctgctggtg ccctgcgcta atcccgtttc aacaacctcc aggatgcgtt ctgtcgccgg
   106021 ataattgcct tgagttttgt gttctgtttt cttgccgacc attttgaaca gcagcgcacg
   106081 acctaacggc cccgccagaa tacgctcgcg tacaggtaga gggcgggaag atgggcgatc
   106141 cttcttcgcc agctcaacag cggcttccag caaaatggag tgcggaacaa cgtcatccac
   106201 cagccccagc tttaatgcct gtttcgcccg aagttgtttt ccggtgagaa tcatctctaa
   106261 tgctgtactg acgcctatca gacgcggtaa acgctgggtg ccgcctgaac cgggtaacaa
   106321 tccaagttgt acttcaggca aaccgagcac cgttttggga tcgtcagtac aaacgcgacc
   106381 gtggcacgcc agcgccagct ccagcccacc gcccaggcaa gcgccatgaa tagccgcgat
   106441 aaccggaatg ggcaaagcat gaatctccgc catcaactgt tggccctgcc gcgccagtgc
   106501 ttccgcttct tgcgccgttt tgcagttgcc aatcatgttg atgtctgctc cggcgataaa
   106561 attgtccggt ttcgcggaga taaacaccac gccgcgcaac tctttgtttt cacggagttg
   106621 cttaataatg gcgcgcacct gcgaggcaaa ttccgccttc agggtattca ttttctcatc
   106681 cggtacgtcg atggtgatga tagcaatgtt gtccagacga acattaagag taaacgctga
   106741 tgccatttcc attattccgc ctccagaacc attgccgcgc caagcccacc ggcagcacag
   106801 gcggtaacta aaccaaatcc accgccgcga cggcgaagtt catgcaatgt ctgggtaatc
   106861 atccgcgcgc cggtcgccgc gaagggatgt ccgtaagcaa tcgaaccgcc aagcacgtta
   106921 aatttgctat cgtccacttc gccagtggca tgtgcacgcc ccagtacttc acgagcaaaa
   106981 cgttcactac ccagcaactg aatattcgcc agtgtctgag ctgcaaaggc ttcgtgcata
   107041 tcgatcaatg tcagttcgct catcgtcaaa ccggcacgtt ccagcgccag cggtgttgac
   107101 caggctggac cgagcaacat gtcctgccag acatcaatcg cagtaaatgc gtagctgcgc
   107161 agatacccca gcggcaccag ccctaattct ttcgcccggg attcagtcat caggatcacc
   107221 gctgccgcgc catcggtcag cggcgtactg tttgccgccg ttaccgttcc gtgtttgcga
   107281 tcaaacgccg ggcgcagctt tgcgtaatcg gcaagcgagg aattaccgcg aatattgttg
   107341 tcttcgacaa gcggttgttt ataaggaggg ataaaggcag tcatcacctc ttctttgagt
   107401 tttccgtctg accatgcctg agcggcacgc tgatgcgaac ggtgcgctaa tgcatcttgc
   107461 tgttctcggg tgatgccgta ggttttcgcc atttgttcgg cggtgtcgcc catccgcaag
   107521 ccagtagaat attctgctac cgcaggcggt acgggcatta agtcgcgcaa acgcaggcga
   107581 gagaagagtt tcaggcgctg gctcatggtt cgagctttgt tgacatcaac cagcacgtgc
   107641 gccagttttt tactgacgcc aattggcaat acggaagagg aatctgcccc accggcaatc
   107701 cccgctcgga tagttcccgc catcaggctt tctgcgacgt ttgcaaccgc ctggaaactg
   107761 gtagcgcaag cgcggctgac gctgtaagca tcggtatgca cattcattcc ggtaccgaga
   107821 acaatttcac gcgcaatgtt gggggcttca ggcatttgta cgacctgacc aaagaccagc
   107881 tgttcaatca cttcagcggg gatctcgctg cgtgccagca gttcgcctac caccatcttc
   107941 cctaaatcaa ccgcgggaat gccatgaaaa gccgtcgcct ggcgggcaaa aggcgtacgt
   108001 aaaccgctaa caatggcgat acgatcgccc tggcgggtaa ccagtggtaa aacctgaccc
   108061 ataacactcc cctgtaaaaa aataaataaa gtggtctgac ctgatcatag tattaaccat
   108121 ttttttacat ttagccaagc ggagaaaaag gaaagtggga gctatgacac agtgaagaca
   108181 gaagaaagaa aagaaaacgc ccctgccagc aactgacagg ggcgtacgta cccgagagga
   108241 attaacgcag acccagctgg aagatcagca tttcagcttc gcaggcaaaa gtgaaatcga
   108301 tatccaggcg cacaccgtca gactcttcag tgaaagtcgg ggagattttg cacggttcgg
   108361 attccacgct acgggatttt tcagtcagcg ccgccagcgt ttgttctgct tctgcgcggt
   108421 ttgcaaacac gcggctgtaa gacgcggtgc agtcggagtt gtccataatg gtgccaacat
   108481 ccatacagca gcaaaccggg gtttcatcag cactacattt actcatcgtt gatttcctct
   108541 gtatgtgcac ccaaggtgcc agataaacgt tgtggatatt ttacgcttcc ggaaagtgct
   108601 gctccagttg ttaattctgc aaaatcggat aagtgaccga aatcacactt aaaaatgatc
   108661 taaaacaaaa ttcacccgaa tccgcgagtg cgcaacctcc aaattttgcc agctggatcg
   108721 cgtttcttag atcatatttg aaaaaagatg gaaacatact tgcaacattc tagctggtcc
   108781 gacctatact ctcgccactg gtctgatttc taagatgtac ctcagaccct acacttcgcg
   108841 ctcctgttac agcacgtaac atagtttgta taaaaataaa tcattgaggt tatggtcatg
   108901 agccagaaaa ccctgtttac aaagtctgct ctcgcagtcg cagtggcact tatctccacc
   108961 caggcctggt cggcaggctt tcagttaaac gaattttctt cctctggcct gggccgggct
   109021 tattcagggg aaggcgcaat tgccgatgat gcaggtaacg ttagccgtaa ccccgcattg
   109081 attaccatgt ttgaccgccc gacattttct gcgggtgcgg tttatattga cccggatgtg
   109141 aatatcagcg gaacgtctcc gtctggtcgt agcctgcaag ccgataacat cgcgcctacg
   109201 gcatgggttc cgaacatgca ctttgttgca ccgattaacg accaatttgg ttggggcgct
   109261 tctattacct ctaactatgg tctggcaaca gagtttaatg atacttatgc aggcggctct
   109321 gtcgggggta caactgacct tgaaaccatg aacctgaact taagcggtgc gtatcgctta
   109381 aataatgcat ggagctttgg tcttggtttc aacgccatct acgctcgcgc gaaaattgaa
   109441 cgtttcgcag gcgatctggg gcagttggtt gctggtcaga ttatgcaatc tcctgccggg
   109501 caaactcctc aagggcaagc attggcagct accgccaacg gtatcgacag taataccaaa
   109561 atcgctcatc tgaacggtaa ccagtggggc tttggctgga acgccggaat cctgtatgaa
   109621 ctggataaaa ataaccgcta tgcactgacc taccgttctg aagtgaaaat tgacttcaaa
   109681 ggtaactaca gcagcgatct taatcgtgcg tttaataact acggtttgcc aattcctacc
   109741 gcgacaggtg gcgcaacgca atcgggttat ctgacgctga acctgcctga aatgtgggaa
   109801 gtgtcaggtt ataaccgtgt tgatccacag tgggcgattc actatagcct ggcttacacc
   109861 agctggagtc agttccagca gctgaaagcg acctcaacca gtggcgacac gctgttccag
   109921 aaacatgaag gctttaaaga tgcttaccgc atcgcgttgg gtaccactta ttactacgat
   109981 gataactgga ctttccgtac cggtatcgcc tttgatgaca gcccagttcc ggctcagaat
   110041 cgttctatct ccattccgga ccaggaccgt ttctggctga gtgcaggtac gacttacgca
   110101 tttaataaag atgcttcagt cgacgttggt gtttcttata tgcacggtca gagcgtgaaa
   110161 attaacgaag gcccatacca gttcgagtct gaaggtaaag cctggctgtt cggtactaac
   110221 tttaactacg cgttctgata acgcgttcgc ctggataaag tcacctgcta tgcaggtgac
   110281 tttaactccc ccacttcacc gaaagtagtt tcctcattgc ttgcctcctg agttttgcaa
   110341 acagcctgat ggcagcttgc cctttcaaat cagtaagtgg tatccacaga caaacctgaa
   110401 gggaaatgca tattttcaag cgttctgctc gtccttctca aagagtttac ttttctgcat
   110461 ttccaggata ctcaaccccc ctggctattg tgcgcttata cactcaaatt aaagataggt
   110521 tctaaataaa tgagcgtttt ttgatagtct atttcattag gtaatatata tttgtaacaa
   110581 atcaatcaac atagaataaa atcatgctac catctatttc aatcaacaat accagcgcag
   110641 cttacccaga atccatcaat gaaaataaca atgatgaaat taatggatta gtacaagagt
   110701 tcaaaaacct ttttaatggt aaggaaggaa taagcacctg tattaaacat ctacttgagc
   110761 ttataaaaaa cgccatacga gtaaacgacg atccttatag atttaatatt aataattcct
   110821 cagttactta tattgatatt ggctccaatg atacagacca tattactatt ggtatcgaca
   110881 accaagaacc aatagaatta cctgcgaact ataaagacaa agaactcgtc cgtactatca
   110941 ttaatgacaa catagttgag aagactcatg atatcaataa caaggaaatg atcttcagcg
   111001 cattaaaaga aatatatgat ggagatcctg gttttatctt cgataagata tcacacaaac
   111061 tcagacatac ggtaacggaa tttgatgaga gcgggaaaag cgaaccaacg gacttattta
   111121 cctggtacgg taaagataaa aaaggcgact ctctcgctat tgtaattaaa aataaaaacg
   111181 gaaatgatta cttatctctc ggttactacg atcaggacga ctaccacatt caaagaggaa
   111241 ttcgtattaa tggtgatagt ctcacccaat attgtagtga aaacgccagg agtgcttcag
   111301 cgtggtttga aagcagtaaa gctatcatgg cagaatcatt tgcaactggt tccgatcatc
   111361 aggttgtaaa cgagctcaac ggggaaagac tgagagaacc taacgaagtt tttaaacgtt
   111421 taggtcgagc aataagatat aattttcaag tggacgatgc aaaatttaga cgcgataatg
   111481 taaaagaaat aatttctact ttattcgcta acaaagttga cgttgaccat cctgaaaata
   111541 aatacaaaga ctttaaggat ctcgaagata aagttgaaaa aagacttcaa aattgccagg
   111601 cgaaatatca aaatgaaatt aatcaactat ctgcactagg tgttaatttt gatgatattt
   111661 aaaattattt ttccagtttt ttgatttttc aacgcctgaa tatcggaaat agt
//
LOCUS       DAJVQH010000003        86252 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJVQH010000003 DAJVQH010000000
VERSION     DAJVQH010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 86252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.003"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..1592)
                     /locus_tag="OQM87_000241"
     CDS             complement(<1..1592)
                     /locus_tag="OQM87_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1506518.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDKNEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQA"
     gene            complement(1576..1932)
                     /locus_tag="OQM87_000242"
     CDS             complement(1576..1932)
                     /locus_tag="OQM87_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCS1506519.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(2055..2237)
                     /locus_tag="OQM87_000243"
     CDS             complement(2055..2237)
                     /locus_tag="OQM87_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506520.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2221..2661)
                     /locus_tag="OQM87_000244"
     CDS             complement(2221..2661)
                     /locus_tag="OQM87_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1506521.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2661..2957)
                     /locus_tag="OQM87_000245"
     CDS             complement(2661..2957)
                     /locus_tag="OQM87_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCS1506522.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(2954..3292)
                     /locus_tag="OQM87_000246"
     CDS             complement(2954..3292)
                     /locus_tag="OQM87_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCS1506523.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3289..4500)
                     /locus_tag="OQM87_000247"
     CDS             complement(3289..4500)
                     /locus_tag="OQM87_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1506524.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4502..5074)
                     /locus_tag="OQM87_000248"
     CDS             complement(4502..5074)
                     /locus_tag="OQM87_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCS1506525.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5114..6271)
                     /locus_tag="OQM87_000249"
     CDS             complement(5114..6271)
                     /locus_tag="OQM87_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCS1506526.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6571..6795)
                     /locus_tag="OQM87_000250"
     CDS             complement(6571..6795)
                     /locus_tag="OQM87_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506527.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(6937..7098)
                     /locus_tag="OQM87_000251"
     CDS             complement(6937..7098)
                     /locus_tag="OQM87_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCS1506528.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(7108..7413)
                     /locus_tag="OQM87_000252"
     CDS             complement(7108..7413)
                     /locus_tag="OQM87_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506529.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7410..7688)
                     /locus_tag="OQM87_000253"
     CDS             complement(7410..7688)
                     /locus_tag="OQM87_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506530.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7968..9716)
                     /locus_tag="OQM87_000254"
     CDS             complement(7968..9716)
                     /locus_tag="OQM87_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1506531.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9713..10012)
                     /locus_tag="OQM87_000255"
     CDS             complement(9713..10012)
                     /locus_tag="OQM87_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1506532.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(10018..10251)
                     /locus_tag="OQM87_000256"
     CDS             complement(10018..10251)
                     /locus_tag="OQM87_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506533.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(10253..10473)
                     /locus_tag="OQM87_000257"
                     /pseudo
     CDS             complement(10253..10473)
                     /locus_tag="OQM87_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10466..10687)
                     /locus_tag="OQM87_000258"
     CDS             complement(10466..10687)
                     /locus_tag="OQM87_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506534.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10677..10877)
                     /locus_tag="OQM87_000259"
     CDS             complement(10677..10877)
                     /locus_tag="OQM87_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCS1506535.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10886..11632)
                     /locus_tag="OQM87_000260"
     CDS             complement(10886..11632)
                     /locus_tag="OQM87_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506536.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11690..11878)
                     /locus_tag="OQM87_000261"
     CDS             complement(11690..11878)
                     /locus_tag="OQM87_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1506537.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(12273..13502)
                     /locus_tag="OQM87_000262"
     CDS             complement(12273..13502)
                     /locus_tag="OQM87_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1506538.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13751..14872
                     /gene="roxA"
                     /locus_tag="OQM87_000263"
                     /gene_synonym="ycfD"
     CDS             13751..14872
                     /gene="roxA"
                     /locus_tag="OQM87_000263"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCS1506539.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14921..16147)
                     /gene="pepT"
                     /locus_tag="OQM87_000264"
     CDS             complement(14921..16147)
                     /gene="pepT"
                     /locus_tag="OQM87_000264"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCS1506540.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16397..17533
                     /gene="potA"
                     /locus_tag="OQM87_000265"
     CDS             16397..17533
                     /gene="potA"
                     /locus_tag="OQM87_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCS1506541.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17517..18374
                     /gene="potB"
                     /locus_tag="OQM87_000266"
     CDS             17517..18374
                     /gene="potB"
                     /locus_tag="OQM87_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCS1506542.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            18371..19165
                     /gene="potC"
                     /locus_tag="OQM87_000267"
     CDS             18371..19165
                     /gene="potC"
                     /locus_tag="OQM87_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCS1506543.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            19162..20208
                     /gene="potD"
                     /locus_tag="OQM87_000268"
     CDS             19162..20208
                     /gene="potD"
                     /locus_tag="OQM87_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCS1506544.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(20364..21185)
                     /gene="cobB"
                     /locus_tag="OQM87_000269"
     CDS             complement(20364..21185)
                     /gene="cobB"
                     /locus_tag="OQM87_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCS1506545.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCHFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(21201..22112)
                     /gene="nagK"
                     /locus_tag="OQM87_000270"
     CDS             complement(21201..22112)
                     /gene="nagK"
                     /locus_tag="OQM87_000270"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCS1506546.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(22141..23385)
                     /gene="lolE"
                     /locus_tag="OQM87_000271"
     CDS             complement(22141..23385)
                     /gene="lolE"
                     /locus_tag="OQM87_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCS1506547.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(23385..24086)
                     /gene="lolD"
                     /locus_tag="OQM87_000272"
     CDS             complement(23385..24086)
                     /gene="lolD"
                     /locus_tag="OQM87_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCS1506548.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(24079..25278)
                     /gene="lolC"
                     /locus_tag="OQM87_000273"
     CDS             complement(24079..25278)
                     /gene="lolC"
                     /locus_tag="OQM87_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCS1506549.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25540..26586
                     /locus_tag="OQM87_000274"
     CDS             25540..26586
                     /locus_tag="OQM87_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCS1506550.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26714..30160
                     /gene="mfd"
                     /locus_tag="OQM87_000275"
     CDS             26714..30160
                     /gene="mfd"
                     /locus_tag="OQM87_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCS1506551.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            30303..31265
                     /gene="ldtC"
                     /locus_tag="OQM87_000276"
                     /pseudo
     CDS             30303..31265
                     /gene="ldtC"
                     /locus_tag="OQM87_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(31348..31605)
                     /gene="bhsA"
                     /locus_tag="OQM87_000277"
     CDS             complement(31348..31605)
                     /gene="bhsA"
                     /locus_tag="OQM87_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCS1506552.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31846..32478
                     /gene="comR"
                     /locus_tag="OQM87_000278"
     CDS             31846..32478
                     /gene="comR"
                     /locus_tag="OQM87_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCS1506553.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32540..33079)
                     /locus_tag="OQM87_000279"
     CDS             complement(32540..33079)
                     /locus_tag="OQM87_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1506554.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(33306..34610)
                     /gene="ndh"
                     /locus_tag="OQM87_000280"
     CDS             complement(33306..34610)
                     /gene="ndh"
                     /locus_tag="OQM87_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCS1506555.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(35011..35553)
                     /locus_tag="OQM87_000281"
     CDS             complement(35011..35553)
                     /locus_tag="OQM87_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1506556.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35576..36601)
                     /gene="nagZ"
                     /locus_tag="OQM87_000282"
     CDS             complement(35576..36601)
                     /gene="nagZ"
                     /locus_tag="OQM87_000282"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCS1506557.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36612..37436)
                     /gene="thiK"
                     /locus_tag="OQM87_000283"
     CDS             complement(36612..37436)
                     /gene="thiK"
                     /locus_tag="OQM87_000283"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCS1506558.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(37417..38058)
                     /gene="lpoB"
                     /locus_tag="OQM87_000284"
     CDS             complement(37417..38058)
                     /gene="lpoB"
                     /locus_tag="OQM87_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCS1506559.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(38073..38450)
                     /locus_tag="OQM87_000285"
     CDS             complement(38073..38450)
                     /locus_tag="OQM87_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCS1506560.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(38453..38812)
                     /gene="hinT"
                     /locus_tag="OQM87_000286"
     CDS             complement(38453..38812)
                     /gene="hinT"
                     /locus_tag="OQM87_000286"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCS1506561.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            39146..39970
                     /locus_tag="OQM87_000287"
     CDS             39146..39970
                     /locus_tag="OQM87_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1506562.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPT"
     gene            39974..41335
                     /gene="fhuE"
                     /locus_tag="OQM87_000288"
                     /pseudo
     CDS             39974..41335
                     /gene="fhuE"
                     /locus_tag="OQM87_000288"
                     /inference="COORDINATES: protein motif:HMM:NF007447.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(41395..42828)
                     /gene="ptsG"
                     /locus_tag="OQM87_000289"
     CDS             complement(41395..42828)
                     /gene="ptsG"
                     /locus_tag="OQM87_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCS1506563.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(43123..43920)
                     /locus_tag="OQM87_000290"
     CDS             complement(43123..43920)
                     /locus_tag="OQM87_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1506564.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43931..44935)
                     /gene="holB"
                     /locus_tag="OQM87_000291"
     CDS             complement(43931..44935)
                     /gene="holB"
                     /locus_tag="OQM87_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCS1506565.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAALVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44932..45573)
                     /locus_tag="OQM87_000292"
     CDS             complement(44932..45573)
                     /locus_tag="OQM87_000292"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCS1506566.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(45563..46585)
                     /gene="yceG"
                     /locus_tag="OQM87_000293"
     CDS             complement(45563..46585)
                     /gene="yceG"
                     /locus_tag="OQM87_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCS1506567.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(46588..47397)
                     /gene="pabC"
                     /locus_tag="OQM87_000294"
     CDS             complement(46588..47397)
                     /gene="pabC"
                     /locus_tag="OQM87_000294"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCS1506568.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47517..48758)
                     /gene="fabF"
                     /locus_tag="OQM87_000295"
     CDS             complement(47517..48758)
                     /gene="fabF"
                     /locus_tag="OQM87_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCS1506569.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48845..49081)
                     /gene="acpP"
                     /locus_tag="OQM87_000296"
     CDS             complement(48845..49081)
                     /gene="acpP"
                     /locus_tag="OQM87_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1506570.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(49292..50026)
                     /gene="fabG"
                     /locus_tag="OQM87_000297"
     CDS             complement(49292..50026)
                     /gene="fabG"
                     /locus_tag="OQM87_000297"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCS1506571.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGSQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(50039..50968)
                     /gene="fabD"
                     /locus_tag="OQM87_000298"
     CDS             complement(50039..50968)
                     /gene="fabD"
                     /locus_tag="OQM87_000298"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCS1506572.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50984..51937)
                     /gene="fabH"
                     /locus_tag="OQM87_000299"
     CDS             complement(50984..51937)
                     /gene="fabH"
                     /locus_tag="OQM87_000299"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCS1506573.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(52005..53075)
                     /gene="plsX"
                     /locus_tag="OQM87_000300"
     CDS             complement(52005..53075)
                     /gene="plsX"
                     /locus_tag="OQM87_000300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCS1506574.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(53156..53329)
                     /gene="rpmF"
                     /locus_tag="OQM87_000301"
     CDS             complement(53156..53329)
                     /gene="rpmF"
                     /locus_tag="OQM87_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCS1506575.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(53381..53902)
                     /gene="yceD"
                     /locus_tag="OQM87_000302"
     CDS             complement(53381..53902)
                     /gene="yceD"
                     /locus_tag="OQM87_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCS1506576.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            54101..54685
                     /gene="yceF"
                     /locus_tag="OQM87_000303"
     CDS             54101..54685
                     /gene="yceF"
                     /locus_tag="OQM87_000303"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCS1506577.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54797..55756)
                     /gene="rluC"
                     /locus_tag="OQM87_000304"
     CDS             complement(54797..55756)
                     /gene="rluC"
                     /locus_tag="OQM87_000304"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCS1506578.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            56329..59514
                     /gene="rne"
                     /locus_tag="OQM87_000305"
     CDS             56329..59514
                     /gene="rne"
                     /locus_tag="OQM87_000305"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCS1506579.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEGVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59711..60664)
                     /gene="flgL"
                     /locus_tag="OQM87_000306"
     CDS             complement(59711..60664)
                     /gene="flgL"
                     /locus_tag="OQM87_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCS1506580.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60676..62318)
                     /gene="flgK"
                     /locus_tag="OQM87_000307"
                     /pseudo
     CDS             complement(60676..62318)
                     /gene="flgK"
                     /locus_tag="OQM87_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(62384..63325)
                     /gene="flgJ"
                     /locus_tag="OQM87_000308"
     CDS             complement(62384..63325)
                     /gene="flgJ"
                     /locus_tag="OQM87_000308"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCS1506581.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(63325..64422)
                     /gene="flgI"
                     /locus_tag="OQM87_000309"
     CDS             complement(63325..64422)
                     /gene="flgI"
                     /locus_tag="OQM87_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCS1506582.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(64434..65132)
                     /gene="flgH"
                     /locus_tag="OQM87_000310"
     CDS             complement(64434..65132)
                     /gene="flgH"
                     /locus_tag="OQM87_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCS1506583.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(65185..65967)
                     /gene="flgG"
                     /locus_tag="OQM87_000311"
     CDS             complement(65185..65967)
                     /gene="flgG"
                     /locus_tag="OQM87_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCS1506584.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(66105..66856)
                     /gene="flgF"
                     /locus_tag="OQM87_000312"
                     /pseudo
     CDS             complement(66105..66856)
                     /gene="flgF"
                     /locus_tag="OQM87_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(66876..68075)
                     /gene="flgE"
                     /locus_tag="OQM87_000313"
     CDS             complement(66876..68075)
                     /gene="flgE"
                     /locus_tag="OQM87_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCS1506585.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTCAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIVATT
                     QNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQ
                     SSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQIL
                     NTRVNLR"
     gene            complement(68100..68795)
                     /gene="flgD"
                     /locus_tag="OQM87_000314"
                     /pseudo
     CDS             complement(68100..68795)
                     /gene="flgD"
                     /locus_tag="OQM87_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(68807..69210)
                     /gene="flgC"
                     /locus_tag="OQM87_000315"
                     /pseudo
     CDS             complement(68807..69210)
                     /gene="flgC"
                     /locus_tag="OQM87_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(69214..69387)
                     /locus_tag="OQM87_000316"
     CDS             complement(69214..69387)
                     /locus_tag="OQM87_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HCS1506586.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            69542..70201
                     /gene="flgA"
                     /locus_tag="OQM87_000317"
     CDS             69542..70201
                     /gene="flgA"
                     /locus_tag="OQM87_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCS1506587.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            70277..70553
                     /gene="flgM"
                     /locus_tag="OQM87_000318"
                     /pseudo
     CDS             70277..70553
                     /gene="flgM"
                     /locus_tag="OQM87_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
     gene            70558..70974
                     /gene="flgN"
                     /locus_tag="OQM87_000319"
     CDS             70558..70974
                     /gene="flgN"
                     /locus_tag="OQM87_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCS1506588.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(71014..72549)
                     /gene="murJ"
                     /locus_tag="OQM87_000320"
     CDS             complement(71014..72549)
                     /gene="murJ"
                     /locus_tag="OQM87_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCS1506589.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSHLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(72716..73582)
                     /locus_tag="OQM87_000321"
     CDS             complement(72716..73582)
                     /locus_tag="OQM87_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1506590.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(73584..74231)
                     /locus_tag="OQM87_000322"
     CDS             complement(73584..74231)
                     /locus_tag="OQM87_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCS1506591.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(74242..74826)
                     /gene="rimJ"
                     /locus_tag="OQM87_000323"
     CDS             complement(74242..74826)
                     /gene="rimJ"
                     /locus_tag="OQM87_000323"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1506592.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            75062..76270
                     /gene="mdtH"
                     /locus_tag="OQM87_000324"
     CDS             75062..76270
                     /gene="mdtH"
                     /locus_tag="OQM87_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCS1506593.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEYR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            76334..76981
                     /gene="grxB"
                     /locus_tag="OQM87_000325"
     CDS             76334..76981
                     /gene="grxB"
                     /locus_tag="OQM87_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCS1506594.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYHDNMAKQTQINLLSSMAI"
     gene            77115..77675
                     /locus_tag="OQM87_000326"
     CDS             77115..77675
                     /locus_tag="OQM87_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1506595.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            77781..78827
                     /gene="pyrC"
                     /locus_tag="OQM87_000327"
     CDS             77781..78827
                     /gene="pyrC"
                     /locus_tag="OQM87_000327"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCS1506596.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            78901..79146
                     /gene="dinI"
                     /locus_tag="OQM87_000328"
     CDS             78901..79146
                     /gene="dinI"
                     /locus_tag="OQM87_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCS1506597.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            79436..79690
                     /gene="bssS"
                     /locus_tag="OQM87_000329"
     CDS             79436..79690
                     /gene="bssS"
                     /locus_tag="OQM87_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCS1506598.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            79805..80923
                     /gene="solA"
                     /locus_tag="OQM87_000330"
     CDS             79805..80923
                     /gene="solA"
                     /locus_tag="OQM87_000330"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCS1506599.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            80971..81084
                     /locus_tag="OQM87_000331"
     CDS             80971..81084
                     /locus_tag="OQM87_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCS1506600.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            81345..81911
                     /locus_tag="OQM87_000332"
     CDS             81345..81911
                     /locus_tag="OQM87_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCS1506601.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            81915..82490
                     /locus_tag="OQM87_000333"
     CDS             81915..82490
                     /locus_tag="OQM87_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCS1506602.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(82532..83584)
                     /locus_tag="OQM87_000334"
     CDS             complement(82532..83584)
                     /locus_tag="OQM87_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCS1506603.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            83809..84729
                     /gene="lpxL"
                     /locus_tag="OQM87_000335"
     CDS             83809..84729
                     /gene="lpxL"
                     /locus_tag="OQM87_000335"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCS1506604.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            84901..85098
                     /locus_tag="OQM87_000336"
     CDS             84901..85098
                     /locus_tag="OQM87_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCS1506605.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            85122..>86252
                     /locus_tag="OQM87_000337"
     CDS             85122..>86252
                     /locus_tag="OQM87_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1506606.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCSECGATIVLGGYALKRFGMYRCPNRSANR
                     CSAKAISRKQTDATILYMLALCDRFETENTDTIDSLKLQREDL"
BASE COUNT        20697 a        22923 c        21235 g        21397 t
ORIGIN      
        1 tctgcctgga atgtaccaaa cgccatcagc gccgccacgc tcgggtctat cttgttcggt
       61 gattttttct tgttcggctt gatattggcg ttcgcgtcac tctgcatcac gacgttactc
      121 atcgaccagg acaacaccgg atcgccacga tgcacaatca ccctgcggtt aacaaaaact
      181 tcgaacgatt tcgccgccgg actgaatctg aggtaggttt gcgggaacgg ctccacctca
      241 aaaccagccc cctgtaattg cgtcctgaga tgcgtggcgt tccacgtatc aaagcccacc
      301 agcctgatat taaatttctc cgcatcctgc atgatgtcat ctctgatccg gtcgtaatca
      361 atgcagtcac ctggcgttgt gcgtatccag cccgctttag cccactggcg atagacagcg
      421 cggtttttat tggcggggtt ctgtagctgg aactccggca gataatgacg ggaaatcagc
      481 ataatggttt taccgaccgg aaaggcatag catacgctgg aaatatcgct ggttgatgat
      541 aagtccagcc ccgcgtaaca ctcctgaccg tgtaaatctt cctccgtgaa cgttccggca
      601 cactcagccc atgcaccatt acccatccac ggggtagtcc cctgacacca gatattaaat
      661 cgctttgtca tcatctccac ccactgcgac ggaatacccc gcgctttctg gatggttgag
      721 gccagttttt cacgatccac ggaaacatca atattagggt tcgcctttat ccacatcgcc
      781 ggatcatcaa cctcgttttt gtcgtccagt tcgtaaatca gtacaaaaat tgaatcgttg
      841 acctcttcgc cgtccaggat ctggcagcaa taatcatagt gctgtttaca ggctgaaacg
      901 acgttactgc ccgatgtggt aatggcaaat aacagccctt cgggacgtgc gcccatcccc
      961 agctcaagcg cggaataaac gccgttatcg gggtgtaggt ggtattcgtc cacgatggaa
     1021 agactggggt ttgtcccctc gatggttgcc gcttttgctg ccagcggctt taacaggctg
     1081 ttgcttttcg ggtgtatcac cttatgcgcc tgaatattca cccgcctgcg taacggtcgg
     1141 gataaaaggc acatctgacg cgcatcatca aacacgatcc gcgcctggtc acggctcacc
     1201 gctgcggtgt agatatcctg ctgaccgttc tccataatca gaaaccagtt agccagaatc
     1261 gcggcggtcg tggatttcgc atttttgcgc ggcacttcga taaaggcgct cgtgtatttg
     1321 cgccgtccgg tggccttaac cttaaagccg aggatgcacg caaaggcgaa ctgctgccac
     1381 ggctccagct caatgggtct gccacgcatc ggtcctttta cgtgcgggca caccctggaa
     1441 aaggcaataa accgctccac aacctcacga tcgaacatgt aaagggggct tttaaggtcc
     1501 gaaaagtacc gtttaacagc ctgttttaac cgtttacagg ccggaatttt gcccgttttt
     1561 acgtcttctg cgtacttatt ccaggcggtc aagctcgtcc tcttcttctg tttccggtgg
     1621 atttttacgg cggcttatcg ggtcaaaacc gagcaaggag gccattttta tcatcactct
     1681 ttcagcgtcg gattttgcgc ttaatgcggg gtttctgctc tcgccgccct gactgttaac
     1741 aatgctgaac ccgcgcgccg caaggtctgc aacggctttc cggtaaatgg agtagttgac
     1801 gcaatacagt tccagattgc tccagtcggc gggggtcagg tctccccgtt ccgcaagctg
     1861 ccgcgatttt tcccgccact gcttcacggc gatatcatcc aggtaggcgg gggctttcgg
     1921 tggtcttgcc atgcttattt ttcgtcagat tatttttcaa aaaattcccg tgcataaaaa
     1981 tttggggagg cgttcggtgc ccggcggggt cgggtttgtc ctgaaaacgc cccccacccc
     2041 gtcatacagc ctcatcagcg attgcggaaa cattccataa cctcgcggtc acggtcggtt
     2101 aatcgcttcg ctgtgatgcg ttctgcgcgt cctgacgctt tatctttatg ccctgtttcc
     2161 tgtgtcttcc atgcgtcacg ctgccttata agtccacgga taaggcggtt ttgttcccgc
     2221 tcattcatca gcgtcataca tccagttatt gcggttagca gcccgttctt cctcttcacg
     2281 aaatccacct gcggcacgct tgctttttgt tgccggatca agccatttcg ttttctggtt
     2341 atgacacgcc tggcacaatg gttgatggtt ccattcgggc cagaagagaa catcatcacc
     2401 gccattaatc gggataatgt gatccaccac cacggcgggc gtatatatcc ccttctcaag
     2461 gcatcgcacg cataacgggt ttttactcag atacatggcg cggtatttgt cccactgtct
     2521 ggaatatcca cgcgcgcggc ggtgtcctcg tctggcatcc tctgcacgcc atgccgcccg
     2581 cctgtgctcc tcacacttgc cggatttaac gcgcctgtta cagcctggtt ctgtgcatcg
     2641 tcttaatggt tgccacggca tcagtacacc cccacatcac ggtaagccgt ccagagtgcg
     2701 cctatcgtca tgggtacgcg tgtttttgcg ttatccgcga caatctggcg attttcatac
     2761 aggtgagcga taaacatcat gcagccaatc tttatggctg gcgtgaacgc cagcccgtca
     2821 tcaaagcgcc tgcctatgtg tatctggcac gcctcaagcg atgcggcaat gtatccgctg
     2881 attaactggt cttcatcgtc gccatcgatg cggcaatgga gtttcacttc ttccagggtg
     2941 ataagttctt ctgtcatttt tccgcgccct cacgacaaag aatttcaagg cgtgtcctgg
     3001 cggcatccgg cagcggctgc ccgatgatat tcagtacacg ccccgccagc ggccccgtac
     3061 tgacctttat ccggctggtg gcgttgatgt ccttccggta ccgtatccag atccttacgg
     3121 taccaatcgc cagctctgcg cccgatgaaa tggcctcctt gctgctgacc atgttcacgc
     3181 ttgcccagag tgtgtgaccg tcctcccacg tttcgagtat ttcgccggtt atatccctgg
     3241 tctgtttcag ggtctgaatc gttatcctgt cacgcagtcg ccctatgttc attccggttt
     3301 ttctccctcg ctgattttta cttcctgttt ccatgcctgg ctgaactcat caccaccatc
     3361 acgcggtgac agcccttcac gttcgcgggc ttcgttcgga cacataacgc cggattttat
     3421 gccgcgctca taggtggcaa agcgttcgcc aggtgtggcc cgtaacaggt ccgcgctgtc
     3481 aaactccacc tgataccgga taccaggtac aggcgaggcc accagcaagg cattttttat
     3541 ctgctgctca aagttcgcca gccacgggcg catcgtcatg gtgagaaatg cgcggcttgc
     3601 ctcgctgaag ttgctgtagg tgctgttgct gtattcctgc aaaaaaatcg gcgagacgtt
     3661 gaacattcgg gcgatgtctt caatggtgaa gcgacgggag gccagccatt cggcatcctg
     3721 attactcatg cccagctgct gatagctcat ccccccttca aggatgggcg tttttccggc
     3781 gtttctggcc cctttgtagc gttccagtgc ggctaatgcc tgtttgccct tcacgccgtc
     3841 cagccattcg cctgacgtga taacccctgc cgccatcatg ccatcacgca tcacgctcgc
     3901 gccgtggcgt tgttgggcca gcccaagccc cagcgattcg cggcagatgg ttacaggtga
     3961 gcgccccata aaaccgtcat ccgtggagta acgaaggtgg agaacctccc acggtaaata
     4021 gttgcgggtg tttccggtgt aggcgtcagt gatgcagtag cgccagttat gatcacctgt
     4081 ctgctccacg ttcaccgact gcggcgggta aggatgtaaa gccgccggaa aaccatcacg
     4141 cccccactga ataaccgcat aagcattacc acttaacagg cagtggcgga tcatcattcg
     4201 cttaaactgg tagggtgtct gccacgcgtt cgggcgctcg ttgaggatgt gatcgaccgg
     4261 atgagaatca agccattcgc gggcctcctt ccccttctca ttgcgtacca ggtacaggta
     4321 acacggcatg gtagccaccg cctcagagat gaccgtgacg gcgttcatga cggcggagag
     4381 tgattccgct gtcccagatg atacgtactc acccgccccc gtgttcgatg tgccggccag
     4441 cgccataaat tcatcaagcg tcatgctgcg ctgctctttt tttcgtctga aaggccacat
     4501 atcacacccc cgctaaatcc gcccaccagc ggcgattatc ctcacgcggc attttttcgg
     4561 ggtgctgctc atacagggaa cggcgggcca gctccacgcc ggaatcgggg taagccggta
     4621 ccgatgtaac ggtaatttcg tacagttccg ccaccagcac ggtgcgcacg catggatctg
     4681 ttgtggtatc ccatacatcc ttacgggagc gaaagctaaa gctcatcccc gagatgtccc
     4741 cacgcttaac cagttcgata acgtcgcgcc ctgtactggt atccggtggg gtcagttcaa
     4801 agcgtaaccc tgtttcgtcc tcttccagtt tcagcgtgcc ggaacgggtg cgccccagta
     4861 acatgctgtg gtcatgctca tacaggccgc gaacgtcatt acccgccgca agccattcag
     4921 taaacgcccc ccgctggaat ttttcgtaaa actcacccca taacagttct gaaagcttat
     4981 cccagcgaac aacgtagccc gtcagcgtac cggctccgct ggtggtgatt tccgatgacc
     5041 ggatttccat actcttcata attttttcac ccataaaaca ctaaaggggc tttttagccc
     5101 cttctgtatg ctgttaatcg tcgtcttccg gcagttccag aatcttgatc gcgttcgaat
     5161 ccaccacgcc accgcctaaa tatttttgcg tgaaaatttt gatgaatcct ggttctgtca
     5221 ggttgtccgg tctggtgcga acacctgttt cgtgatcaac aatgtagtaa ccgcgtttga
     5281 agtcacccag ggcaataacg ttatcaggca taaactccag atattcgacc ggaaggccca
     5341 gcaacgtatc aggatcaccc gcctgtaaac ggtcgcgcca gatataatca ccgttcgcgt
     5401 tcttcacctt ctgaagtttt gccgccgtcg tggagttaac cacccagacc gcgtttttgc
     5461 ggtatttttt acgtaatgca aatttcaggt cgatcagcgg gtccgcagat gtccacgcca
     5521 gagattcgga aggtttaatt acctgcaacg taccaaaatc acgctctttg tcgttcttct
     5581 ctgcacgggg tacggataaa aaaccttttg cttttttgtc accgtcgccc acaaccagat
     5641 cgctttcttc ggtttccgtg aaggtgtcgc caatctcacc cgtcagccat gaaaggatgt
     5701 ccacatcgga aaaatccacg atttcctgtg tggtgcgcgg gtacgcatag accggataca
     5761 gcttaatgct cacctcgtta atctgcgggg tgctggtctg ttcgcgtgcc ttaccctctt
     5821 caccgtggtt aacggtcgca ccgccagcgg aaacaagctg cttaaactcg ttgctgctga
     5881 ttttcttcac ggtacagatg cggcgcatag tggattcatc cgccagcatt cgcatgattt
     5941 cggtgttcag ttcagggata acggtataac caccatcagc gggaacgcct gtacttaatg
     6001 cgcgggtttc accggtcaga atgtagttgc gtagttctgc ggggtcagtg gtctggctgc
     6061 ttttacctgg cttgctgcgc tcttcgtctg caatggcttc aaggcgggaa atgtctttat
     6121 cgagggattc agctttagcg cgtaattcgt caaattttgc gccctcagca tcgttaagac
     6181 tgcggttttc tttttcagcg ttctccagca tgtcgcgcat ctgattttta atggcggttt
     6241 tctgctggcg taattcgatt attctctgca taaaaaagtc ctggggttaa gtaaggaact
     6301 ccaggacgcg gcaaacactc aaccgttttt cataaggaaa tcagtaatcg caccgatcgt
     6361 tttcccgcct ggtaatgaat attggcgagc acattaacag gcgggaaagt ggccccagcg
     6421 tcctggcacc acgggcgaga ataatcatga ttcagttcgg gtaaaatatg ccgatccgct
     6481 cagtgaacaa cgtggaacaa ccacgaacaa ataatttaca aaaaataaca aaaagccgga
     6541 ttgctccggc tgtatgtgct gtatgggtgc tcagtctctg atccctttca gaaattctat
     6601 caatgcatct gtctgttcag gatttactgc cagcatttca ccggatagcg cgcaacgaac
     6661 aaaaccatga tgatcctttt caatcagcgc ccccgtttcc agaaatgcgc ggtaatcagt
     6721 gatattcatc gtctccatgt tgtcagcatg gtatttatca cgctgtttaa gtatctcatc
     6781 aaatttcatc ggcattgcgt tttcctctgt tgtgtctgtt tgtttcagat agtaactatg
     6841 ccggaccgtg acgaaaagcc agtagtgctc cataccgttt caaatgacgc aaaaaaagcc
     6901 ggattgctcc ggcttcgtgg tgtggttctg atattcctac tctgctaatt tgctcagccc
     6961 catgtctgcg taagtgcgat ttactgcatt tctcaggtct gcgtagttct ccgttggcgg
     7021 ctccggtgtg cccttgctgc tctgttttac cagcgggtcg cccaccagcc gcccgtaagc
     7081 tgcaatttgt gctgtcatat tgccacctca gtgaagcgat ccggcattgt tctttttcat
     7141 ctcttccatc attttttcag tgagcattac gcactgaaac cccatgtttt tgatgtctac
     7201 gggtttcatg cctttgtcag tggatatcac aggcagaata tcctgataca catcgcggtc
     7261 agggtaacta agcacacaaa gcgcccatga tgccacgggt gaggatcctt cgtctgtttc
     7321 aacccaccag cctggtgtag ctgcgcttat ttgcagaatc gtgatccgtc cgttgtgctt
     7381 gtaaaatttt tcaccaaatt ttggttcgct catgattcca cctctccggt tttaacgttg
     7441 attgttgtta cctgttccgc ttcggcaatc tcccgctctg tcagcgtggc aaaatttgcc
     7501 gccgtcgtgg tcatgaatgc gcttatcagt tcgggatgtg ctttcgcgta tccttccccc
     7561 gcgtggcggt ctatcgttct gattgccacc tttaaagcgt gctctgtcat gtctaacgcg
     7621 cgatatttcg gttctgttct gtctctgcgt tttttgagtg atttattaaa tttccctgaa
     7681 gtgtgcatat ttatttttac cccctcgttt aaaaagtttt gagttgtgcc tccccttgtc
     7741 taccttagtg gccctcatgc cagtaatggc gctgctttca gcggggtaga gtgcttttat
     7801 ccactatcta ccccgtgtct acctccctgt ctgattcagg taaaatcagg tagagagggt
     7861 agatagtggg tagacagtaa aaaaaggcta cctacctaac ttaatgcact gaattaaatg
     7921 tatttttctt tactcaggta gatagggtag acagcaatta caaaaaatta taaaaacgcg
     7981 tcgcactcgt ctgttgttat tgcgttagtc tgcgttactc ccttaacttt ccgcgtaata
     8041 tattcatgtc cgtaaacttt cgcggctggc ttcatagcct tgccaaagtc atttacgttt
     8101 agcggtttgc tcctgcctgc gtacgccata aacgccagat agacgcggta aaggctgttt
     8161 ctggtcgtgt acttcactga atcgccaccg ccgcccatca tcaggccgcg tgcttcctcc
     8221 agaaaattca ggaactggca aaactcaata accggatccg tctgttgctt tattgccaga
     8281 gcttcatcac cgtcacgctg ttccagtagt aaagcccgtg ctttctcaga gtcggtaaag
     8341 ttcgccagca atcggcggat aatgacaggg atttcagccg caatcttttc cggtagctcc
     8401 ctgtcttttt cggcctcact aacgatattg tcgaaacgga aaatcacgcg acgacgtgcc
     8461 acacctccgg cccgttcggt gaatatcatc gggttattgt tggtcgccag caccaccgcc
     8521 ctgattacag ccgtgaaacg cttttcgtat ttcgggttaa tttccacggg gtcaccgccc
     8581 gtgattttct tgatgcccgt tccttcgcct gtatatttcg gctggtcagc caggacgata
     8641 agacgactcc cgacaacctg cggacgtcca ccagcatcat caagcgatgt catttcagcg
     8701 cttaccgtgt tctgtttccc tgccagaagg ctggctatgt gtgtgaatgt acttttaccg
     8761 ctcccgccgt ctccggtggc ctcaataaac atctgccagt cgtaccggtt cgccataatc
     8821 atgtacagcg cggcacatat acgcatcatc ttgcgctggt cttttccggc tgcgtgctca
     8881 agccatttat gaaagtttag cgcgttatcg cggatgttct cccctggtgc tggtggcgtg
     8941 tactcaatgc cgttgtgcgt ggtgatccag ttctccggcg tgtgcgggga aaattccccc
     9001 gttttcaggt caagcgcacc attggcgaac ggcagcaaat cgccggacgg ctcgcccatt
     9061 ggttcggcaa taacttttaa cgcttccacg gcgttattga ttacgcgctt gctgaaagtg
     9121 gccctgtgct ctgaatagat cgccaccatt tcgcggctaa gttccattgt gctgaccgga
     9181 caccataccc cgccgcgcca tacgtgaacg atttcacttt cctgatgcac acaaacgcca
     9241 tcaaagcgct cggcaagcag ctgcgcgcgc tcactgtctg ccatttgtgc cagttgcgta
     9301 tttttctgct ccggcatggt aagccctgcg gcaatatttt cacgctcagc attcagatag
     9361 cgccgccagt tttcacactt ctggacgtgc attccttcgg ggtaaaaatt tgcgtcctga
     9421 atatctgccg ccgccagctt ctgaccaatc tttttggtct ccaccaaatc cagttctccg
     9481 gcctggtaca gccttacgcg tttttttcca tccggaacaa ttttcagtgc atcaagttcg
     9541 gcaagctgat ttggcccaag ccacacaggc ggcacattat cgccggatgc ggggccatcc
     9601 tgctcctgcc attgtttcgc gtgcgaccag gcatcactac ccgcaaagat aattacctct
     9661 gtgtctttgt gttttattcc gcgtggctgt ttttttacgt tcggtgccag tttcattttt
     9721 tacccctgaa tacgttaagc atctttttta tttcctgaat attggcgcgt gctttctccc
     9781 tgctggttgg tttactgcgg ggcgctgcct gtaccagcgt gaaatcacgc cggaattgat
     9841 aaacaggcat cacgcaatca tattcgtaac cttcacggcg gtaggtgatg cgccgttctg
     9901 ccacgccttt aatcgttacc atgccgccgt actggtcgcg gtaaatatca ccgcgcgtaa
     9961 atttagggtg agtgttgcca ctggcagtta agccagaata tttaagtttc attattttta
    10021 ttctccggtg tgctgttctt tatatctgtc gtgcaataga tctatttctt gcagttccat
    10081 tattacaggc tcaagaagcg ttattaatgc cgtggcaatt cttgattttt gtttgtcgcg
    10141 ttcattgtcg ccaagtgttt caagccatat gcgcaatatt tccagcatgt tttcactgtg
    10201 agaaagtgca agaaatgcgc ggtctattgt ttcgtggtaa atatcacgca tattaatccc
    10261 cgtccgtcgt ttttcttaaa ataacctctg tcacgaaatc agcataatcg gcggcgatat
    10321 tcagtacatc aagcccgttg atttatattc tctcgtggaa agtaagaaaa aagccgctct
    10381 aataagttct ggcattgacg aaagcgcatc agccgcatca tcaggaacgc cggaaaattc
    10441 ctgtttcagg gaattaaaac gatcatcacg cataaccccc cccattttca caatcggcaa
    10501 caagaatatt tttagcgtca ttcagtgacc gcgttgcgat gtatcggata cattccaggg
    10561 cgaactgtgt ccggtctttc tgttcttctc cctgcgcaag ctctgccgtg cattcaatat
    10621 caataagcgc gtgcatcagc gtagtgagtg cggcggctgc tgcgttcggt gtggttttat
    10681 tgtgcatagg tttccccctg acgaatacgg gcggcgaata cagcaacaca accggacggg
    10741 caacggctac gcgcttcgcg ttccgtccaa gcggttacgt ggatgatttg ggattctccg
    10801 gtactcagtg ccagaaaacg ccacacaaag gccgtttgtg tgtgtacaag gtgtggtata
    10861 tgatttacgg caaccataac ggctcctagt ttacgttgtt ggttagaagc cccgttactg
    10921 ctcctacagt gcggggcttt gctttttgtg ttgattgtgt gtaatgtgtc attacacata
    10981 aatacattac atgaggtgta attaacgtgt caacacacaa aaacgaaagg cgtggtaatc
    11041 ctccgttcca gttccgttta gatcctgact tacgggaaaa gatggaaaag gcacaacaac
    11101 aggacggcga tgattctctt gctgcctgga taaagcgaat aatccgcaaa gaattacagc
    11161 aacgcggcat agaacccaaa ggataaaaac tatcagcgcc gtggtgtgag gtactacggc
    11221 gcattgcttt acaggcgcac acaatgacca acgaagaatc aaccgaaaaa acatcacctg
    11281 cacgaaaaag acgacgcaga aagaaagagc atgaatcaga acgattcgcg ccgtgttctt
    11341 ttgcccttga gaagtacctt aaagaatact catcaaagga gagaacttcg cagttatggc
    11401 aacgcactga accagactga ttgcattgcc caccagcctg atagcggcta tcatccccgt
    11461 gcttatgttt gggatcacac acacaaacgg cgcagcgggt tatctgttca gaaaggcggc
    11521 tccatttcgg ggccgctttt tttatgcctg aaaaacccca attttgtggt tttccagttt
    11581 caccagggcg aacgaatccc cgcccacgtt ctggcgtata ttcaatcttc atggttatag
    11641 ctctgtgttc agatgattga tgtgtggcgg ctgcgtgccg ccagcgtgat taatgaactg
    11701 ccttgcagct atccttccag gccagaacct cggataaaga ccagccaacg gaacgaccgc
    11761 caagtttacg acgtgatggg aattgtccgg ccttttccag gcggtagcgg catgagcggc
    11821 taaggcctgt tagcttttcg cattcttttt cacgtataaa ccgatcagtg cttaacacta
    11881 ttgccccctt tcgtttctta aagagttatt tcgtgttcta ttgcgttggg atgtgtctga
    11941 ttgtgtcagg atgattcaga gttggcaaat gttgaggccg tatggtttac agaaagagga
    12001 ataatcagga taaaatcatt taaattcatg ttaatacaaa ggcataaaat attgtttcat
    12061 gccttttttc tcgctattta aagagtgatt cgctagtgta taaaaaacca gtaagacgtt
    12121 aaaaatcagc tacttataaa tctgtacact ttttcacctc ttgttcgtag ttgttccgca
    12181 ttgttgctca ttgttgctca ttgttgctca ttgttgcacg ttgtatctgt tcgcatatcc
    12241 agtatgcgca tactgaaaaa acacgaaaaa aattattttc ttctggctac tggtagcgtg
    12301 gttacgtttt catgtgttcc cgccagtatc cctaaccgct ccgtccacat atccagcgca
    12361 ttgcgtttag catccagata acgggaatga ttataaactc gctgcattcc tggcatctgg
    12421 tggcctgtaa gctgctccac gacgtgagga tccacgccta aatcgttcag catcgttgta
    12481 aaggtgcgcc ggatgtcatg tagtgaccag tgagggtgat ttagcctcct gtgcgctaat
    12541 ctgccgtact gcgacacgct tgtttcctgt ttcacttccc ccagcaataa gcccgtgtgc
    12601 ctgttctgct ccaccagctg cgtgacgaac ggcagtattg cttccggtat gggccggaat
    12661 attgccacct tcgttttgct gtgttctttc ggcacggtcc agagcatttc ggtaaaatcc
    12721 cactcgctga tctccgataa cctcagttct accgtccggc atccgaacac aatcaggagg
    12781 cggattaacg cgatgtagta gggggagaat atttttttgt ccagtgcctg caataattcg
    12841 cccagttctt tggtgcttaa gacacgctcg cttatatccg gttttttccc aacgtccgcc
    12901 acactcatat catcaagaac gttgctgatt gcatagcgcc gcctccggca gaacttaagc
    12961 gcctgtttgc acgtctgtag caagaatccg gcagtaacag gcgttcgctt tgccacctgg
    13021 tcaaaacagg ccagccagtg ccgtagctcg catttatcca gcggcatagc accaatgtgc
    13081 tgtattacgt gattattaag gcgctttttc agggcggcat aatccacgcg gttttccttt
    13141 gcgtacgact caagccagta ggtgagcgca tcgccaaccg ttaccggctt taacgcttcc
    13201 tgtacggtgt aattaagctc atgacgtgga tttttcccct ctgccagcca tgcgcgacac
    13261 tgggcggctt tttccctggc tgatttcagg ctcagatcag gataatttcc cagcttaatg
    13321 cgttccggtg gtgcccccct tcccgttccg gccctgtaag tgaaatacca ggttaaaagg
    13381 ccactggttg aatgcctgac gctcaggttt ccaccgtcat taagaaaggc tgttttttgg
    13441 gcgggtgtgc cgttgatttt cctcagctgt gtatcgctca gtttgttaag tgctctgctc
    13501 ataattttga ttccggtcat cacaattaac tacaccatta actacaccga tcgttgcaca
    13561 aagggctaca acgtgaaaca agctggaaca aggaaaatca caaatctgtt tataatcaaa
    13621 aacataatga acaatctgaa acattatgaa acggcaaaaa acactaattg ataaaatatg
    13681 tccatacttc acgcattacg ttaagcatcc gttataatcg gttgcagata ccagcctgtg
    13741 gatgcttaac atggaatacc aactcactct taactggccc gattttcttg aacgtcactg
    13801 gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat tttattgacc cgatctctcc
    13861 agacgagttg gcgggtctgg cgatggaaag cgaagttgac agtcgactgg tcagtcacca
    13921 ggatggcaaa tggcaggtca gccacggccc gttcgaaagc tacgatcatc tcggtgaaac
    13981 caactggtca ttactggtac aggcagtaaa ccactggcat gagccgaccg ccgcgctgat
    14041 gcgaccgttc cgtgaactac cggactggcg tattgatgat ctgatgattt ctttttctgt
    14101 acctggcggc ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca ttcagggtac
    14161 cggacgtcgt cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact gcccacatcc
    14221 agatctgtta caggtcgatc cgttcgaagc catcatcgat gaagagctgg agcctggtga
    14281 tattctttat attccgccag gattcccgca tgaaggctac gcgctggaaa atgcgatgaa
    14341 ctattccgtg ggttttcgcg cgccaaatac gcgggaatta attagcggat ttgccgatta
    14401 tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat ccggatgttc cacctcgcgc
    14461 tcatcctgcg gatgttctgc cgcaagagat ggataaactg cgtgagatga tgctcgaatt
    14521 gatcaaccag ccggaacact ttaagcaatg gtttggcgag tttatatccc agtcacgtca
    14581 tgaactggat atcgcgccgc cggaaccgcc ttatcagcca gatgaaatct acgatgcact
    14641 gaaacaaggt gatgtgctgg tgcgcctggg tggtctgcgc gtattgcgca ttggcgacga
    14701 cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt ccggcactgg atgcactcgc
    14761 cagcaacatt gcgctgactg cggagaattt tggcgatgcg ctggaagatc cgtcattcct
    14821 cgcgatgctc gcggcgctgg tcaatagcgg gtattggttc ttcgaagggt aagtttggat
    14881 ttaagccgga tgcagcatca catgccgcat cccttttcgc ttacttccgt tgcgccgtta
    14941 actcggcaat acggacgatc acctgcaccg ctttttccat accttccaga atcacaaact
    15001 catgcttacc atgatagttg taaccgccag tgaacaggtt cgggcaaggt aatcccataa
    15061 acgacaactg cgcgccgttg gtaccgccgc ggatcggttt cagttccggt tcaatatcgc
    15121 agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg atgctcaacc actttctcgc
    15181 gcatattgta gtaactgtct tcaatcacca attcaatgta gcaatcagga tgtaaccctt
    15241 tgcccacttt tttggcgatc tccatcattt tacgtttacg cgcttcaaac tgtttacggt
    15301 cgaaatcacg gatgatgtag tgcatatcgg cccgttcaac ggtgcctttc atgctcgcca
    15361 ggtgatagaa accttcatag ccttctgtca tttccgggct ttcatccgcc ggaacttccg
    15421 catgaatacg tgccgccagc gacagcgcat ttaccatcac tcctttcgcc gtgcccggat
    15481 gaacattgtt accgacaatt ttgatattga ccgatgcggc gttgaagttt tcaaactcca
    15541 gttcgcctac gccaccaccg tcaacagtgt aagcccagcg ggcatcgaag gcatcaacat
    15601 caaaatgttt cgcccctttg cccacttctt catccggggt aaaggcgacg cgaatatcac
    15661 catgcggaat gtttttctgt tgcaataccg ccagcgcggt catgatttct gcaatacctg
    15721 ctttgtcatc ggcacctaac aaggttttac catcggtggt aatcaacgtc tgacccagta
    15781 gctgatgcag caccgggaac ataaccggtg ataaaacttc atcgccgata cccagcgcaa
    15841 tatcgccacc gcgatagttt tcaacaattt gcggattaac atttttgccg ctgcaatccg
    15901 gtgaggtatc cacatgagaa ataaagccaa tcgccgggat atcgccaggg acgttagccg
    15961 gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac attgataagc cccatttctt
    16021 cgagctgctc tttcagcaga tgcaataact tccattggcc ttccgtgctg ggaacctgtc
    16081 tcacccctgc ttttgattgg gtatccagag acacgtagtt caaaaatcgc tcaagtagtt
    16141 tatccatgta gtcaccctca ctttttgtga caacattatt aagaagcaag aaaagacaaa
    16201 tattgcgtca ggtcactttt acccctgcaa gcgggaatat ttatcagcat taccttaatg
    16261 aatataaagc taagccagta attcacaaca aagattggcg attcaagcgg tttgccgtag
    16321 aattaccgcc ctcattaaga gtcaccaagg tggttaacca caaaccccgc atcggtaagc
    16381 catccgttgc gtttacatgg gacagagtaa aaaattgaat aaacaaccga gttcgctttc
    16441 accgctggtg caattggcgg gaattcgcaa atgctttgat ggtaaagagg tcattcccca
    16501 gctggatctg actatcaaca atggcgagtt cctcacgctg cttggccctt ctggctgcgg
    16561 taaaacaacc gttcttcgcc tgattgcagg tctggaaact gttgattccg gacgcatcat
    16621 gctggataac gaggacatca cccacgttcc ggcggaaaac cgctatgtga acactgtttt
    16681 ccaaagctat gcacttttcc cccacatgac cgtgttcgaa aatgtggcct ttgggttgcg
    16741 catgcaaaaa acccccgctg ctgaaattac gccccgcgtg atggaagccc tgcggatggt
    16801 gcagctggaa accttcgctc aacgtaaacc gcatcagctc tctggtggtc aacagcaacg
    16861 cgtcgccatt gctcgcgcgg tggttaacaa gcctcgtctg ttgttgctgg atgagtcgct
    16921 ctcagcgctg gattacaaac tgcgtaagca aatgcagaac gagctgaaag cgttacagcg
    16981 taagcttggc attacattcg ttttcgtgac tcacgaccag gaagaagcac tcaccatgtc
    17041 ggacaggatt gtggtgatgc gcgatggtcg cattgagcaa gacggcacgc cgcgtgaaat
    17101 ctacgaagag ccgaaaaacc tgtttgttgc cgggttcatt ggcgaaatta atatgtttaa
    17161 cgccacggtg atcgagcgac tcgacgagca gcgcgtacgc gccaacgttg aaggccgcga
    17221 atgtaatatc tacgttaact tcgccgttga accggggcaa aaactgcatg ttctgctgcg
    17281 cccggaagac ttacgtgttg aagagattaa cgacgacaac cacgctgaag ggctgattgg
    17341 ttacgttcgt gagcgtaact acaaaggcat gacgctggag tcggttgttg aactggaaaa
    17401 tggcaagatg gtgatggtca gcgaattctt caatgaagac gatcctgact ttgaccactc
    17461 tctcgaccaa aaaatggcca ttaattgggt agaaagctgg gaggtcgtac tggctgatga
    17521 agaacacaag taagttccag aatgtagtga ttgtcactat tgtcggttgg cttgtgttgt
    17581 ttgtctttct gcccaacctg atgatcattg gcaccagctt tttgacccgc gacgacgcca
    17641 gtttcgtcaa aatggtcttt acgctggata actacacgcg tctgctcgat ccgctctatt
    17701 ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc caccctcgcc tgcctggtgc
    17761 tgggctatcc atttgcctgg tttctggcga agctgccaca caaggtgcgt ccgctgctgc
    17821 tgtttctgct gattgttccg ttctggacca actcattgat tcgtatctac gggctgaaaa
    17881 ttttcctcag caccaaaggc tatctcaacg agtttttgct ctggctgggc gttatcgaca
    17941 caccaatccg tatcatgttc acgcccagtg cggtgattat cggtctggtt tacattctgc
    18001 tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa actggataag ccgttgcttg
    18061 aggcggcgcg cgatctcggt gccagcaagt tacagacttt tatccgcatc attattccac
    18121 tgacgatgcc ggggattatt gccggatgtc tgctggtgat gctgccagcg atgggcctgt
    18181 tctatgtgtc cgacctgatg ggcggtgcga aaaacctgct gatcggtaac gtcatcaagg
    18241 tacagttcct caatattcgt gactggccgt ttggtgcagc caccagcatt acgctgacta
    18301 tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc tcgtttgctg aataagaagg
    18361 tggaactcga atgatcggtc gactgcttcg cggcggtttt atgaccgcta tctacgcgta
    18421 cctgtatatc ccaatcatta ttttgattgt gaactccttt aacagctcgc gctttggcat
    18481 caactggcag ggttttacca ccaaatggta tagcctgctg atgaacaacg atagcctgtt
    18541 acaggcagcg cagcattcac taacaatggc ggtgttttcg gcgacgtttg ctacgcttat
    18601 cggttcactg acggcagttg cgctgtaccg ttatcgcttt cgcggtaagc cgttcgttag
    18661 cggaatgctg tttgtggtaa tgatgtcacc agatatcgtg atggcaattt cgctgctggt
    18721 gctgtttatg ctgctgggta ttcagcttgg cttctggtcg ctgctgttct cgcatatcac
    18781 cttctgcctg ccatttgtgg tggtgacggt gtattcgcgc ctgaaaggtt ttgacgtgcg
    18841 gatgctggaa gcggcgaaag atctcggtgc cagcgaattt accattctgc gaaaaatcat
    18901 tctgccactg gcaatgccag cggtggcggc gggctgggtg ttaagcttta ccctgtcgat
    18961 ggacgatgtg gtagtttctt cattcgtcac cggaccaagt tatgaaattc tgccgttaaa
    19021 aatttattcg atggtcaaag tcggcgtatc gccggaagtt aacgcgctgg caaccatatt
    19081 actggtgctg tcgctggtga tggttattgc cagccagctt attgctcgtg ataaaacgaa
    19141 aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc acctgctcgc ggcgggtgct
    19201 ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca acacgctgta tttctacaac
    19261 tggaccgagt acgtgccgcc aggactgctt gaacagttca ccaaagaaac cggtattaag
    19321 gttatctatt cgacttacga gtcgaacgaa accatgtacg ccaagctgaa aacgtacaaa
    19381 gacggtgcct atgacctggt ggttccttca acctattacg tcgataaaat gcgtaaagag
    19441 gggatgatcc agaagatcga caagtcgaag ttaacgaact tcagcaatct cgatccagac
    19501 atgctcaaca agccttttga cccgaataac gactactcca ttccgtatat ctggggtgcg
    19561 acggcgattg gtgttaacgg tgatgcagtg gatccgaaat ctgtcaccag ctgggccgat
    19621 ctgtggaagc cagagtacaa aggcagcctg ctgttgaccg acgatgcccg tgaagtgttc
    19681 cagatggcgc tgcgtaagct gggctactcc ggtaacacca ccgatccgaa agagattgaa
    19741 gctgcatata acgagctgaa aaaactgatg ccaaacgtcg cagcgtttaa ctccgataac
    19801 ccggctaacc cgtacatgga aggcgaagtt aacctcggca tgatctggaa cggttctgct
    19861 ttcgttgcac gccaggcggg tacgcctatt gacgtggtgt ggccgaaaga aggcggcata
    19921 ttctggatgg acagcctggc gatcccggca aatgccaaaa acaaagaagg cgcgctgaaa
    19981 ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg ttgctgaaac tatcggttat
    20041 ccaacgccaa accttgcggc gcgtaagctg ttaagtccag aagtggcaaa cgataagacg
    20101 ctctacccgg atgctgaaac cattaaaaat ggcgaatggc agaatgacgt tggcgcagcc
    20161 agcagcattt atgaagagta ttatcagaag ctgaaagcag gacgttaatt caacaagcgg
    20221 acggtggtat taccgcccgt tttttagtta gcaaggatgt ggctaatgag tcaggacagt
    20281 aaagttttat cccgctcttt cgtcggtatt gtggtccgct tgtaggcctg ataagcgtag
    20341 cgcatcaggc aatgcttcct gctttataat ccctccagca acttttcaac aaattctggc
    20401 accacctggc ttgccgggcc gtaatatttc tcggcaaatt cattaccaac ctgactcggt
    20461 tcaagattca gttcaacggt gtgagcacca tgtagtttgg cttcgtgtac aaacccagca
    20521 gccggataga catgcccgga agtaccaatg gcaataaaga catccgccat cgacaacgcc
    20581 atataaattt catccatgcc gagtggcatt tcgccaaacc aaacgacgtg cggacgcaag
    20641 ggtgccggga agtggcaaca atggcattta tcttctggcg taacgtctcc ggtccagtcg
    20701 agaacctgac cactttgcga acaacgaact ttcagcaatt ccccatgcat atgaatgaca
    20761 ttggtattac ctgcgcgttc atgcaggttg tctatattct gcgttaccag caaaaagcga
    20821 tcgccgagag cgtcttgcag tttagccagc gcaagatgcg cggcgttagg ctgaatttct
    20881 ggctgctgca gctgtcgacg acgggcgtta taaaacgctt gcaccagttc aggatcgcga
    20941 tcgaaacctt ccggcgttgc cacatcttca acccgatgtt cttcccacag gccatctgcg
    21001 gcgcgaaagg tacgaatacc tgattccgca gaaattcctg cccctgtcag tacgagtact
    21061 cttggttttt ccattgcttc cggcaccact ttatctctga aaaaaatacg ctgacgcaaa
    21121 cgctcgcgca ggcgacgttt atttttacga aaacgactta accgatgacc ccgacgcgac
    21181 agcatagcaa cctctgttgt ttaatcggtt agatgtagga aggccgcacc acgcattcct
    21241 cccgcatcac cgtggcgcgc gcgttcaatg cgcggaacac gagctacagg taagagatga
    21301 caaggcaacc tgtccgccag ttgcgttgtg attgccggga aattcgataa gccaccacca
    21361 atgacgacca ggtcagggtc aacaatggtc aggatatttc ccagagaaac cgctaataaa
    21421 tccagataac gctcaacgtg cgcccttgcc tgctcatcgc cttgatcata aagcgcaata
    21481 atttcgggag cctgcaacgg ttgatgataa tagtgttgat acagccacgc aaaaccgcga
    21541 ccagacagat aattttcaat gcagccatgc tgaccacagc cgcagcggcg taacgggaaa
    21601 tccaacccca tcatggttaa cgcatcaacc ggcagacgca tatggccaaa ctcgccggta
    21661 atgtagcttt taccagtaat cggtttgccg ttgaaaacca gcccgccgcc aacgccggtg
    21721 ccgagaatta accccatcac cagtggatat tgcgtaaatt catcctccca ggcttctgaa
    21781 agggcaaaac agttggcatc gttatcaagg cgtacatcgc gatcaagacg tgcgctcagg
    21841 tcggcacgca gcggtttgcc gctggcagcg gggacattag cggcatacag cttaccatct
    21901 tctgtttccg gcattcccgg aataccgatg ccgacagaac ctttacagcc aaaacgttgg
    21961 tcagcttcag ctaccagctc acacactgca tctaaaaatg cgtcatagct gtcacgcggt
    22021 gtcggcaccc gcttttccca ctgcaactgc cgaccgctat caaacacgcc aagcgcaatt
    22081 tttgttccac caatatcaaa cccgtaatac attgccgctc cttgttttaa tgtactgcct
    22141 ttactggccg ctaaggactc gcgcagggtc aatattactg gcgcgccgcg ccggatacca
    22201 acttgccaaa agactcagca acaatgctgt gaccagtacg tagaagacgt ccagccagtg
    22261 caattccgat ggcaggaagt caataaaata gatatcgctg gagaggaact gatgaccgat
    22321 cagcttttca atccactcaa taatcggggt aagttgcagt gaaacaacta cgccgataac
    22381 caccccacac aggctgccga acagccctgc cagcaatcca taccagacaa agatggcgcg
    22441 aattaaacca tctttcgccc ccagcgttct taatactgcg atatcgccac tcttgtcttt
    22501 caccgccatc actaaggtgg agacgatgtt gaaacaggcc acgccaatca ccagtaccat
    22561 cgccagatac ataatggcgc ggatcatctg gatatcgcga tacatatagc cgtaagtacc
    22621 aatccaactt ttaatataaa catagctgtt ggtcacttca cccgcatcgc gtaccagctt
    22681 attggcgttg aaaacatccg tcattttaag ggcaatacct gacacgctgg aacccatatc
    22741 aagatattgt tgggcatccg ccagcgggat catggcaaaa ctgtgatcga gttgaccact
    22801 caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt ggctgcatca gtttatgctc
    22861 gggattcgag ttggggatca tgatcgacac ccaatcgccc tgcttcactt tcagcgcatc
    22921 cgccacgcct ttgccgatga taatttgctg ttcgcccgct ttaaaattgc gccacgcatc
    22981 cccctgaaca aacgagggta atgcgctcag acgctgttcc tgttgtgggt taacgccctt
    23041 cacctggatt gcgcgcagat tcgctccact ttccaccaga ccggtgaaat tgatatacgg
    23101 cgcagcggcg gcaatacccg gcactttctg cacgttatcc agtgcttcct gccagttagt
    23161 ccatggctga tttaccgctt cgatttcgcc atgcggcacc accgccagaa tgcggttatt
    23221 cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg atcaataccg ccacgccgag
    23281 ggcaatgcca atggtagaaa tgacagagat tagcgacacc atgccgccgc gtcgccgtcc
    23341 gcgactaaaa cgcaggccaa tcaataacga taaaggcatc gccattactc cgcccccatc
    23401 aggctcagtt ccgccgtcag acgtccatca cgcatttcca gttggcggct catacgtttc
    23461 gccagttgca ggtcgtgagt gaccaccagg aaagcggtgc cctgcaagcg attcaactct
    23521 ccaagcaact ggaagatgct gtcggcgtta cgcgcatcga ggttaccggt aggttcatcc
    23581 gccagtacca gacgcggatt attgaccagc gcacgggcaa tcgctacacg ctggcgttcg
    23641 ccgccagaaa gttcagatgg gcggtgattc gcacgatgct ccagccccac cgcttttaac
    23701 atctccagtg cacggctgtt gatttcagcg ggctttttct tgccaatcag cagtggcata
    23761 gccacgtttt ccagggcagt aaaatccggc agcaggtggt gaaactgata aataaagccc
    23821 agcttctggt tgcgcagttc tgctttcgcc gctgaagaca gtttgctcat cggctgaccg
    23881 ttaaagatca catcgccgga ggttggcgta tccagcccgc ctaacaggtg cagcaaggta
    23941 cttttaccgg aaccagagct accgacaatt gccatcattt caccttcgcc cacgctgaaa
    24001 ctgacattgt gcagcacatc ggtttgcaca ctgccttcct gatagcgttt gcacaggttg
    24061 tcgcattgca acaggatctt attcataacg taaagcctca gcgggttgtg tggcggcagc
    24121 gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc atcgccacca gcgcaataac
    24181 aatgacctgt aaaggttcga tagccaccgg cagcgccgcg ccatcaagca ggacgccgat
    24241 tatcggcatc agattattta actggctggc aagcagtgcg ccaagcgccg ctccgaggat
    24301 tgcaccgata atcccggcgc tggccccttg caccataaag accatcatga tttgtcgcgg
    24361 agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc ttctccatca ccatcagccc
    24421 cagcgaggta ataatgttaa acgccgcaac agcgacaatc aggctcagca gtaagcccat
    24481 catatttttt tccatgcgta cggcctggaa cagctcgcct ttacggtcac gccagtcctg
    24541 ccatttgctg ccttcaggca gtttttgctg acttaaagag tcaactttca gcggctcatc
    24601 cagccacaaa cgccagccgg taatattggc tgccggataa cgcatcagac gcgaggcatc
    24661 ctcaatattc accagcattt catagccatc gacttcactg ttggcggcga aagtgccaat
    24721 cacattgaac aggcgctgac ttggaatacg ccccatcggc gtgaactggc tggcagatgg
    24781 caccatcacg cggatttgat caccgcgatt gacgcccaac tgtgaggcaa gttgttcgcc
    24841 gaggatgaca ttatatttcc ccggctcgag gtcagtttgt ttcacattga ccagatacgg
    24901 cgttagtgga tctttttgcg ccgggtcgat accgagcatc accccgaccg ccacgctgcg
    24961 cgcgctttgc agtaccacat caccggtagt aataggtgcg acgcgattaa cgccgtccag
    25021 tttgactgcc gtttccggga gttgctgcgg gttaagagag ccatgctcag aagagagaat
    25081 tgcctgtggc atcaggccaa ggatgttgtt ttgcagctcg cgctcaaagc cgttcatcac
    25141 tgacaatact gtgaccagcg ccatcacccc gagggtaatg ccgatggtag aaagccagga
    25201 gacgaaacga ccgaagcgat ccgctgcacg cccacgcatg taacgcaagc caataaatag
    25261 agcgacaggt tggtacatga aatccgtctg gttgctgtag caaagacacg agtatataag
    25321 cgaaagcaag atgggtaaac gtctgaactg taagtgatcg tcagggctaa tctgaaaagc
    25381 ccccgtaaat caatcggcta aattgccatg ttttatcatc aatcttgccg cctgatctta
    25441 atctgaaaaa tcatccaata cagacagata cccattacac ctcgggtcat aaccagcagg
    25501 atcaaccgca tgtaaattaa aaaagagcgt gttgttctga tgaaacaaaa agagctatgg
    25561 attaaccaga tcaaagggtt atgtatttgt ctggtggtga tttatcactc ggtcattacc
    25621 ttttatccgc atatgaccac ttttcagcat ccgttatcgg aagtcctgag caaatgctgg
    25681 atctatttca atctttacct tgcccccttt cgtatgccgg tttttttctt tatatccggc
    25741 tatttgattc gccgctatat cgacagcgta ccgtggggaa attgtctcga taaacggatc
    25801 tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc acctgagcgc
    25861 gatttaagca atgcctccaa tgccgcttat gccgattcca ccggtgagtt cctgcacggg
    25921 atgatcaccg ccagcaccag cttgtggtat ctgtatgcgt taattgtcta tttcgtggta
    25981 tgtaaaattt ttagccgtct ggcgctgcca ctattcgcct tgtttgtact gctgagtgtg
    26041 gcggttaatt tcgttcccac gccgtggtgg ggaatgaaca gtgtgatacg caatttgcct
    26101 tattacagcc ttggcgcatg gtttggcgca acaataatga cctgtgttaa agaggtgccg
    26161 ttgcgccgcc atctgctgat ggcttctttg cttaccgttc tggcggtcgg tgcctggttg
    26221 tttactatct cgctgctgtt gtcgctggta tcgattgtgg tgatcatgaa gctgttttat
    26281 cagtacgagc aacgattcgg tatgcgctcc accagcctgc tgaatgtgat tggctccaac
    26341 accattgcta tctacaccac ccatcgcatt ctggttgaaa tattcagctt aactctgctt
    26401 gcgcaaatga acgcagcacg ctggtcgccg caagtcgaac tgacactcct gctggtttat
    26461 ccctttgtca gtttgttcat ctgtactgtt gcgggcttgc tggtaagaaa actttcacag
    26521 cgtgcattca gcgatctgtt gttctccccg ccttcactgc ccgctgccgt cagttactct
    26581 cgttaaacca tcaccccagc cggggcgatt caatttgcta aaccatgtcg attacggata
    26641 atgaacagca ttgcttatca gagatatgcc cccatatgtt gaggcatatc ctaacgagaa
    26701 tctgacaacc gttatgcctg aacaatatcg ttatacgctg cccgtcaaag cgggtgagca
    26761 gcgtctgctg ggcgagttaa ccggcgcagc ctgtgcaacg ctggtagcgg aaattgccga
    26821 acgtcacgcc ggtccggtgg tactcattgc accagatatg caaaatgctc tgcgtttgca
    26881 tgatgaaatc agccagttca ccgatcagat ggtgatgaat ctggcggact gggaaactct
    26941 tccctacgac agtttttcgc ctcatcagga cattatctcc tcgcgccttt ccacccttta
    27001 ccagctaccg acgatgcagc gtggcgtact gattgttccg gtgaatacgc ttatgcagcg
    27061 cgtttgccca cacagttttc tccacggtca tgcgctggtg atgaaaaaag gtcagcgcct
    27121 gtcacgagat gcattacgaa cccaactgga cagcgccggt tatcgctatg ttgaccaggt
    27181 gatggagcac ggcgaatacg ccacgcgcgg cgcgttgctg gatctcttcc cgatggggag
    27241 tgagctgcct tatcgtcttg atttctttga tgatgaaatc gacagcctgc gggtgtttga
    27301 cgtcgacagc cagcgcacgc tggaggaagt agaagcgatc aatctgctgc ccgcacacga
    27361 atttccgacc gataaagcgg caattgaact gttccgcagc cagtggcgcg ataccttcga
    27421 agtgaagcgc gatccggaac atatttacca gcaagtgagt aaaggcacat tacctgccgg
    27481 gatcgagtac tggcagccat tgttcttcag cgaaccactg ccgccgctgt tcagttattt
    27541 ccctgccaat accttgctcg tgaatactgg cgatctggaa accagtgccg aacgtttcca
    27601 ggctgacacg ctggcgcgtt ttgagaatcg cggcgtcgat ccgatgcgcc cgctgttgcc
    27661 accacaatcg ctctggctgc gggtggacga gctcttctca gagctgaaaa actggccccg
    27721 agtgcagcta aaaactgaac atttaccgac aaaagccgcg aatgccaatt taggtttcca
    27781 gaaactgcca gacctggccg ttcaggcgca acaaaaagcg ccgctggatg cgctgcgtaa
    27841 gttcctcgag actttcgacg gtccggtggt gttctcggta gaaagtgaag gtcgccgtga
    27901 agcgctgggt gaactgctcg cacgaattaa aattgctccg caacgcatta tgcgtcttga
    27961 tgaagccagc gaccgtgggc gttatctgat gattggcgct gccgaacatg gttttgtcga
    28021 taaggtgcgt aatctggcgc tgatctgcga aagcgatctg ctcggtgaac gtgttgcccg
    28081 ccgtcgtcag gattctcgcc gcaccatcaa ccccgataca ctgatccgta accttgcgga
    28141 gctgcatatt ggtcagccgg tggtccatct ggagcacggc gtcggtcgct atgccggaat
    28201 gaccacgctg gaagctggcg gcattactgg cgagtacctg atgctcacct atgccaacga
    28261 cgccaaactg tatgttccgg tgtcgtcact gcatctgatt agccgttacg caggtggcgc
    28321 ggaagaaaac gccccgctgc ataaacttgg cggcgatgcg tggtcacgcg cgcggcagaa
    28381 agcggcggaa aaagtgcgtg atgtggcggc ggaattgctg gatatctacg cgcaacgcgc
    28441 cgccaaagag ggcttcgcgt ttaaacacga tcgtgagcag tatcagttgt tctgcgacag
    28501 cttcccgttt gaaaccacgc cggatcaggc gcaggccatt aatgcggtac ttaacgacat
    28561 gtgtcagccg ctggcaatgg atcgtctggt gtgcggcgat gttggctttg gtaaaacaga
    28621 agtggcgatg cgcgccgctt tcctggcagt agataaccac aagcaggtag cggtgttggt
    28681 gcctaccacc cttcttgcgc agcagcatta cgacaacttc cgcgaccgtt tcgccaactg
    28741 gccggtacgt atcgaaatga tctcccgttt ccgcagcgcc aaagagcaga cgcaaatcct
    28801 tgcggaagtg gcggaaggga aaatcgatat tctgatcggt acgcacaaac tgctgcaaag
    28861 tgacgtcaag tttaaagatt taggcctgct gattgtcgat gaagaacacc gcttcggggt
    28921 gcgtcataaa gagcgcatta aagcgatgcg cgcgaacgtg gatattctga cgcttactgc
    28981 aacgccgatc ccacgtacgc tgaatatggc aatgagcgga atgcgtgacc tgtcgattat
    29041 cgccacgccg cccgcccgtc gtctggcagt taaaaccttt gtccgtgagt atgacagcct
    29101 ggtggtccgg gaggcgatcc tgcgtgaaat tttgcgcgga ggacaggttt attatctcta
    29161 caatgatgtg gaaaacattc agaaagccgc cgaacggctg gcagaactgg tgcctgaagc
    29221 acggattgcc atcggtcacg ggcagatgcg cgagcgcgaa ctggaacggg tgatgaatga
    29281 tttccatcat caacgtttca acgtgctggt ttgtaccacc attatcgaaa ccgggatcga
    29341 catcccgaca gccaacacca ttatcattga acgtgcggat cacttcggtc tggcgcagct
    29401 gcaccagtta cgcggtcgcg tcggacgttc acatcatcag gcatatgcat ggctgctgac
    29461 gccgcatcca aaagcgatga ctaccgatgc acaaaaacgt cttgaagcga ttgcctcgct
    29521 ggaagatctc ggggcaggtt ttgcgctggc aacgcacgat ctggagattc gcggcgcggg
    29581 tgaactgctt ggcgaagaac aaagcggctc aatggaaacc atcggtttct cgctgtatat
    29641 ggagttgctg gaaaacgccg tcgatgcact gaaagccgga cgcgagccgt cgctggaaga
    29701 tctcaccagc cagcaaacag aagtcgagct gcggatgccg tcgctattgc cagatgattt
    29761 catccctgac gtgaacacgc gtctgtcgtt ctacaaacgt attgccagcg ccaaaacgga
    29821 aaacgaactg gaagagatca aagtcgagct tatcgatcgc ttcggcctgc tgccagatcc
    29881 ggcgcgtacc ctgctggata ttgcccgtct gcgccagcaa gcgcagaaac tggggatcag
    29941 gaagctggaa ggtaatgaga aaggcgggat gatcgaattt gccgagaaga atcacgttaa
    30001 tccggcctgg ttgattggtt tgctgcaaaa acagccgcag cattaccgcc ttgatggtcc
    30061 gacgcgcctg aaatttattc aggatttgag tgagcggaaa acgcgtatcg aatgggtacg
    30121 ccagtttatg cgtgaactgg aagagaacgc gatcgcttaa tgtaaatcgg gggcgtttct
    30181 gcgcccccat ctgcaacatt tacaaattct ttgcactttc ctgcactatc cgacactgtc
    30241 accatccata attcaggctt atctgtttat tacaataacc ttatatttat tatggatttt
    30301 tggtgatgat caaaacgcgt ttttctcgct ggctaacgtt ttttacgttc gccgctgccg
    30361 tggcgctggc gctaccggca aaagccaaca cctggccgct gccgccagcg agcagtcgtc
    30421 tggttggcga aaacaaattt catgtggtgg aaaatgacgg tggttctctg gaagccatcg
    30481 ccaaaaaata taacgtcggc tttctcgctc tgttacaggc taaccccggc gttgatcctt
    30541 acgtaccgcg cgcgggcagc gtgttaacga tcccgttgcc aaccctactt ccagacgcgc
    30601 cgcgcgaagg cattgtgatc aacattgcgg agctgcgtct ctattactac ccgccgggta
    30661 aaaattcggt aaccgtgtat ccaataggta ttggtcagtt aggtggtgac acgctgacac
    30721 cgacaatggt gaccaccgtt tcagacaaac gtgcaaaccc aacctggacg ccaacggcaa
    30781 acatccgcgc ccgttataaa gcacagggaa ttgagttgcc tgcggtagtg ccggctggac
    30841 cggataaccc aatgggccat catgcgattc gtctggcggc ctatggcggc gtttatttgc
    30901 ttcacggtac gaacgccgat ttcggcattg gcatgcgggt aagttctggc tgtattcgtc
    30961 tgcgggatga cgatatcaaa acactcttta gctaggtcac cccaggcacc aaagtgaata
    31021 tcatcaacac tccgataaaa gtctctgccg aaccaaacgg tgcgcgtctg gttgaagtac
    31081 atcagccgct gtcagagaag attgatgacg atccgcagct gctgccaatt acgctgaata
    31141 gcgcaatgca atcatttaaa gatgcagcac aaactgacgc tgaagtgatg caacatgtga
    31201 tggatgtccg ttccgggatg ccggtggatg tccgccgtca tcaagtgagc ccacaaactc
    31261 tgtaaatcct ggataaaaaa aatccccgcg acacggcatg tcgcggggaa aaaatcagca
    31321 gtagcaggca ttaatgaggg ttaatgctta tttataaatt actgctgttc catggagggt
    31381 attcggaccg gttacagaag taatacggaa agattttgcg cccatctcat ccgctttttg
    31441 cgccagctgc tcttccagcg atcccagatt tgtccccgcg ttagcactga tagtaccgac
    31501 tttttgttgg ccttctggcg ttgactgaac ttcgacagcc gcaaagttgg caaatgacat
    31561 ggagcttaaa atcgccgcag cgatgagggt ttttacgttt ttcataatag tggccttatg
    31621 cagatgaatg acgtcgcaag aaattactta acgatcgata actaaatgat agatgtgatc
    31681 tggatcacat acaagatatt ttttataaca atcattaatt aatttaaaaa tctttaaatt
    31741 tcagtcaaat agaaagcaat tatttttatg ggatctggac tggtgaacgg aggtgattat
    31801 ttttataata acctttcatt cacagtcacc aggtacaacg gcatcatggc aactgactca
    31861 acacaatgtg taaaaaaaag ccgtggccgc ccaaaagtgt tcgacaggga tgccgcgctt
    31921 gataaggcca tgaaattgtt ctggcaacac ggttatgaag cgacttctct tgcggacctc
    31981 gtcgaagcga ccggagccaa agcgcccacg ctatacgcgg aatttaccaa caaagagggg
    32041 ttatttcgtg ccgttctcga ccgctatatc gatcgttttg ccgctaagca tgaagcacag
    32101 ctgttttgtg aagagaaaag cgtggagtct gcgctggctg actattttgc tgccatcgcc
    32161 aactgcttta ccagcaaaga caccccggca ggctgcttca tgatcaacaa ctgcaccacc
    32221 ctctccccag attcaggaga tatcgccaat acgttgaaat cacgccatgc gatgcaagag
    32281 cgcactttgc agcagttttt atgtcaacga caagcgcgcg gggaaatccc gacccactgt
    32341 gacgtgacac atctggcaga attccttaat tgtattattc aggggatgtc gatcagcgca
    32401 cgcgaaggtg catcgctgga aaaactgatg cagattgccg gaacgacttt gcgtttatgg
    32461 cccgaactgg tgaaataagt cgcaaaaaaa gcccctcagc ctgagcgaat gaggggccaa
    32521 attgcagagt acagccttgt tatactttgt tattcaaaat cagttgcccg ttactatcta
    32581 gcgggatctg cgtacccgga tcgcggtcca tgcggatttt gccctgctga tcgccaatct
    32641 tataggtcac atcataaccg agcatttttt ctgacttgtc atacaccgtt ttacaacgct
    32701 gttgcgtagt cgtgtaagta tcgctttcct ggagagagcc ctggatctgg ttaccggcat
    32761 atccaccacc cagcgccccc acaacagtgg cgacatcttt accgcgacca ccaccaaact
    32821 gatgccctat cacgccacca gcaacagcgc cgagcaccga cccggtaatg cgattttcat
    32881 cctgcaccgg tcgacgatgg gtcactgtga cgttgcgaca ctcctgacgc ggtgttttaa
    32941 ccgtttcctt gattggggtt gcagaaacaa cctgagcgta ttgcgggccc cgttcaaaca
    33001 cgttcagact ggccactgcc gctacgccca acgcagctgc gacaccaatc ccgatacccg
    33061 ccaacattga tttattcacg ggaacacctc cttctttgca gttatgccaa tcatgcaagc
    33121 gaaggagaaa agcagctatc agacagaaga tgaaaagcgc gggaatgact ggaaatactt
    33181 aaggattcgg aggagtctga aggaatgcct gatgcgacgc ttaacgcgtc ttatcaggcc
    33241 taccgctcgt accatttgtc cagggcggat aaggcgttta tgccacatca gccagtgtac
    33301 gtcgattaat gcaacttcaa acgcggacgg ataacgcggt taatactccc caccagcatc
    33361 attaatccgg ttttaaagta accatgcagc gcaatctgat gcattcggta tagcgagata
    33421 tatacaaagc gcgcaattcg tccttcaatc atcattgagc cgcgcgtcag gttacccatc
    33481 aggctaccaa cggtggagaa gttcgacagc gataccagcg aaccatgatc tttatactga
    33541 taatttttca gcggcttacc gttcatctgc gccagaatgt tgttcattgc gcaagtcgcc
    33601 atctggtgtg cagcctgagc acgcggcgga acaaagcccc cttccggacg cgggcatgac
    33661 gcgcagtcgc caatagcgta aatgtctgga tcgcgagtgg tttgcagcgt cggttccacc
    33721 accagctggt tgatacggtt agtttcaaga ccaccgatat ctttcaggaa gtctggcgct
    33781 ttgatcccgg ctgcccatac catcagatca gcctcaatat attcgccatc tttagtgtgc
    33841 aggccgcctt catcagcact ggtgaccatg gtttgcgtca gcacgcgaac gccaagtttc
    33901 gttagctcgt tgtgggccgc agcagagata cgtggcggta atgcaggcaa aatacgttct
    33961 cccgcttcta ccagcgttac gttcagggct tcgttggtca ggcctttgta accgtagctg
    34021 tgcagttgct tgatcgcgtt gtgcaattca gcggagagtt ctacacccgt cgcgccgccg
    34081 ccgacaatcg caatgttcac tttgccattt gcgcccaggt tggcagagta tttcaggaac
    34141 agattcagca tctcctggtg gaagcgacgc gcctggtgcg ggttatcgag gaaaatgcag
    34201 ttctctttga cgcctggcgt attgaaatcg ttagaggtgc tacccagcgc catgaccagg
    34261 gtgtcatagg cgattttacg ttccggaacc aacagttcac ctttctcgtc gcgtagttct
    34321 gcaatagtga ttgttttcgc ttcacgatca atatcaatga cggaacccaa ctggaactgg
    34381 aaaccatgat tgcgcgcatg ggccagatag ctcaacgcat cgacgccttc atcaagcgag
    34441 ccagtcgcca cttcgtgcag cagtggtttc cacaggtggc tgtggttacg atcgaccagc
    34501 gtaattttgg cttttttctt acgtcccagc ttatgcccca gctgtgttgc catttccagc
    34561 ccaccagcac cgccgccgac aatcacaatc tttttcaatg gcgtagtcaa cgtgaccccc
    34621 ttaaatttat taaccaattg ttaattaatg gttattaaca tagctcttaa ttaacaacag
    34681 gttacagcaa tgaaaatgtt cttcgctatt gagaataaca tgaatggtgc attggtcata
    34741 ccaaaattga tgttaatcaa gttttgttgc ttaaaagata ggcaacttta ccgggggaga
    34801 tcaaaaaata gcgtttatta tttgagcaaa ttcggaagag taatataggg tgaagcgtat
    34861 atcgagggcg tttggtcgtc ctgataagat gcgcagcatc gcatcaggca ttgatgctcg
    34921 actgtcggat gcggcgtgga cgccttatcc gacttacaga attcggagcg catcaggtac
    34981 cggcaaggtt acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg
    35041 ctgtaaatgc ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc
    35101 gtaataatga tgcaactctt cagatgtccg ctggctgtta agtgcttcat cattacgcga
    35161 caaaataacc agacaacgat cgcgattctt ctcgcggaag ttggtcacac acttagtggc
    35221 aatatcggca tactcttccg ggcgatcaat cttgccttcc atgttctcat aagggaacaa
    35281 attagggttg aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt ccgcccagta
    35341 tccgcccagg ccaacgccgc aaattagcgg acgctcgtca acgttcagtt gcaacatttt
    35401 gtccacttct ttaagcagat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat
    35461 caagcgtaca tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggact
    35521 gttagagtca aaaccgtgta aatagataat catcgcttcc tcacataagc cagggttagt
    35581 gacctgcttt ctcttcctgc cagcgttcat gtaactgatt cagacgggtg ctgatcgctt
    35641 tccagcgagc cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg
    35701 taacacgttc tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat
    35761 tattgcagac caggatcata tcgcaacccg catccagtga tgcctgcccg cgttcggcat
    35821 aactgcccat aatcgcggca ccttccatcg ataaatcgtc agagaaaatc acgccgtcaa
    35881 aacccagttc ctgacgcaaa acggttttca gccagtaggg agaaccgctc gccggacgcg
    35941 gatcaacatc actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga
    36001 ttaaggaact gaagaccgac atatctttag cgcgaatctc cgcttgtgga cgtggatcgc
    36061 acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag tgtttcccgg
    36121 tcgttttcat tccggcttca tgcataccat caataaaccg gctggcaatt gccagggctt
    36181 tttgtggatc ggcatgataa gaacgctcgc caatcgccgc gctgatatgc ccgacatcca
    36241 gcactggcgc aaagctgata tcaatatcca tagcgatcat ttcgctggcc atcagccaac
    36301 ccgcctcttg cgccagtttg ccgccctctt ccattcccaa cagcgcagca aaggattgtg
    36361 ctgccggtaa gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct tcctgatcta
    36421 ccgccaccac cagatgattg cgcgatgctg cgcggatctg gcgcaccagt tcacgtaact
    36481 gggcaggatc atgatagtta cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcca
    36541 gtatttcacg ctcttccgcg tccagttcgt aaccttcgac atccaacatt actggaccca
    36601 cactgctctc cttattgttt tattaatagc tgccgccagg tgtcatcggc cagcctgata
    36661 aattgttgat cgccggtttg tcgccagcgg tactcaaacc accctccttt gagcatcagc
    36721 agccagggaa accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga
    36781 gtggcatagt cattgaccaa ttgccggtgc tgttcagtat tttccaccca caccgccgcc
    36841 agttccagcg cgatatcacc atctccagca tactcccagt cgatgagttt taaccctgat
    36901 gcgctatgca ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggt
    36961 cgtggttccc gcgctttgcg cagacgtttt aacattcgca gccaacccac agtccgccgc
    37021 gccggatcgc tttgctgcca gtacagttcc agtaagggca acagcgttat tcgccagcca
    37081 aaacgtggtt gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa
    37141 tacgttttta ccgcgccggg cagatagtcg actaccatcc agtcacggag atataaatgc
    37201 ggcttcggtg caatgcttgc gggtagttgt gataaagccc gatactggcg caagaacgcg
    37261 gactgcggcg catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctgatgttca
    37321 atgagaaaac taccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg
    37381 aaaaagcgcg acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa cggcaccttt
    37441 acctgaccag ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc
    37501 gttaacgttg ccagaggcgc tggagtacag cacgtaatga gcgccgacat tgcgggcaat
    37561 gcctatggct ttactacggg tgcctaaact gtcctgcggc gacaaaccta actgttgctt
    37621 agccatcgac agttgctggg cggaaaccag ggtaaatttc ccgttattag ccagcgcatt
    37681 tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct
    37741 atcaaccagc aggacgctac ccgcagtcac cccgtcagcc ccaagcatct tactgaccat
    37801 cggctgcatt gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg gtgccgttcg
    37861 atcttcgtgc tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg gcactgtggg
    37921 gacaggctgt tgtggctcgg ctggttgttc cggcgctggt ttcacttctt ctaccggtgc
    37981 aggttcacgt tgccccacac acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta
    38041 gcgactcatt tttgtcatca agattcaccc ctttacaaat atagataaag tctgactttg
    38101 tgcgccccca gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc gggaatggta
    38161 acgctgcgtg gcctttccag aggatgcatc tccagccctc tggcgtcata ccagtaaaaa
    38221 cgataatgaa cggtaacggg ttcttgcctt tcgttataaa gcgttgagga tgctgaaggt
    38281 tgaatatcag acgtcgaaag gacgggcttt tctgcactga tgcccgcagc caataaagat
    38341 gactccatca ccagcgattg ctcatcattc accggaattt ccggatgtga acgacagccc
    38401 accagcagta acaacaccag agacaccagc ccaaagcatc cttttctcat cgttacagac
    38461 ctttatgcgc cagcattggt cccagcggac ggccacccaa caagtgcata tggatgtggt
    38521 aaacctcttg tccgccatgg cggttggtat tcatgatcag acgatagcca tcttcggcaa
    38581 taccttcttg ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc gcctgctcat
    38641 gctcagctga cacgtcgttc acagtcggaa tgaggatatt cggaatgatc agaatatgcg
    38701 ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg tagacgatat
    38761 cggaggggat ctcacgacga ataattttgc tgaatatagt ttcttctgcc acgacatttt
    38821 cctttttcaa atagccatgc gttgcgccat gctacgccgc gccacactgc gagtatagag
    38881 tatggtcgag ttaaacctgc tctttcaacc ttaactcacg atttcttaag caaaaaatcg
    38941 ttttgctgcc gtatatatcg ccattattcc catttctgcc tgcgaatgcg ctgaaaagac
    39001 attctctgtt tggtttacac gacaaatatg aatgcgtata tttctcattt gcatttgcaa
    39061 gcaaaattat tcgcacacta aatataaccg ctcatcaccc tcctccggca tgagcctgac
    39121 gacaacataa accaagagat ttcaaatgct ttcaacacaa tttaacaggg ataatcaata
    39181 tcaagccatc accaaaccgt cactacttgc cggttgcata gcactggcac tattaccttc
    39241 tgccgctttt gctgcaccag ccactgaaga aacggtgatt gttgagggtt cagccacagc
    39301 tccagatgat ggcgaaaatg attacagcgt aacgtctacc tctgcgggta ccaaaatgca
    39361 gatgactcaa cgtgatattc ctcagtcggt cactattgtt agccagcagc ggatggaaga
    39421 tcagcagtta caaacgctgg gcgaagtgat ggaaaacacg ctggggatca gcaaaagtca
    39481 ggcggattcc gatcgtgctc tttattattc ccgcggattc cagatcgata actatatggt
    39541 tgatggtatc cccacctatt ttgaatcgcg ctggaatctg ggcgacgcac tttctgatat
    39601 ggcactgttt gaacgcgtag aagtagtgcg tggcgcgaca ggactcatga ccgggacggg
    39661 taatccatct gcggcaatta atatggttcg aaaacacgcg accagtcgtg aatttaaagg
    39721 cgatgtctcg gcggaatacg gtagctggaa caaagaacgg tatgtggcgg atttacaaag
    39781 cccactcacc gaagacggta aaatccgcgc gcgaattgtc ggcggctacc agaataacga
    39841 ctcatggctg gaccgctaca atagtgaaaa gaccttcttc tcgggcattg tcgatgctga
    39901 tttaggcgat cttacgacgc tttcagccgg ttacgaatat cagcgcattg atgttaatag
    39961 cccaacctag ggcggtttac cgcgctggaa tactgatggc agcagcaaca gttacgatcg
    40021 cgcacgcagt accgcacctg actgggcgta caacgataaa gagatcaaca aggtctttat
    40081 gaccctgaag cagcggtttg ctgatacctg gcaagcgaca ctgaatgcca cccactctga
    40141 agtcgaattt gacagcaaaa tgatgtatgt cgatgcctat gtaaacaaag cggatggtat
    40201 gctggttggg ccatacagta attatggacc tggctttgat catgtcggcg gcaccggttg
    40261 gaacagtggc aaacgtaaag ttgatgcgct ggatttgttc gctgacggta gttatgaatt
    40321 gtttggtcgt cagcacaatc taatgtttgg tggcagttac agcaaacaaa acaatcgtta
    40381 cttcagttca tgggccaaca tcttcccgga tgaaattggc agtttctaca actttaatgg
    40441 caatttccca caaaccgact ggtcaccaca gagcctggcg caggacgata ccacacatat
    40501 gaaatcgtta tatgctgcca ctcgtgtcac ccttgccgat ccgctgcatc tgatcctcgg
    40561 cgcacgttat accaactggc gggttgatac gctgacttac agcatggaga aaaaccacac
    40621 cacgccttac gctggtctgg tgtttgacat caatgacaac tggtcgacct acgccagcta
    40681 tacctctatt ttccagccgc aaaatgatcg tgacagttca ggcaaatatc tggctccaat
    40741 caccggtaac aactacgagc tgggtctgaa atcggactgg atgaatagcc gtctgaccac
    40801 cacgttagcc atcttccgta ttgagcagga taatgtcgct cagtccaccg gtacacctat
    40861 ccccggcagc aacggcgaaa ccgcctataa agcggtggat gggacagtca gtaaaggggt
    40921 ggaatttgaa ctcaacggcg caattaccga caactggcag ctgacatttg gcgcaacgcg
    40981 ctatcttgca gaggataacg aaggaaacgc cgttaatcct aatctgccac gcaccacggt
    41041 taaaatgttc accagctatc ggttgcctgt catgccagag ttgacagtcg gcggtggtgt
    41101 taactggcaa aatcgcgtgt ataccgacac cgtgacaccg tatggcacct tccgcgccga
    41161 gcaaggtagc tacgcgctgg tggatctctt cacccgctac caggtgacga aaaacttctc
    41221 gttacagggg aacgtcaata acctgttcga caaaacctac gataccaaca tggaaggttc
    41281 tatcgtctac ggcgcaccgc gtaatttcag cattaccggc acgtatcaat tctgaataac
    41341 acctgtaaaa aaggcagcca tctggctgcc ttagtctccc caacgtctta cggattagtg
    41401 gttacggatg tactcatcca tctcggtttt caggttatcg gatttagtac cgaaaatcgc
    41461 ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc agtttcttca ggccagcctg
    41521 atccacttta gacacatcag caacgctgac gcgcagacgg gtaatacatg cgtcgaggtt
    41581 agtaatgttt tctttaccac caaatgcagc aaccagagcc ggtgccattt cgctggtacc
    41641 tgttgctttt gcatcttcag tcgcgtcttc acgacccggc gttttcagat ccagtgcttt
    41701 aatcagcacg cggaagatgg tgtagtaaac aatcgcataa ccgataccga cgatcgggaa
    41761 cagccacagt ttgctgctgt taccagacag aacgatgaag tcgatcagac cgtgcgagaa
    41821 cgacgtaccg tcacgcattc ccagaagaat acagattggg aatgccaggc ctgccagaat
    41881 cgcgtggatg atgtacagga tcggcgcaac gaacatgaag aagaactcga tcggctcggt
    41941 gataccggtc aggaacgagg tcagcgccgc ggagatcata ataccgccca ctttcgcgcg
    42001 gttttctggt ttagcagagt gccagatagc aattgcggca gctggcagac cgtacatttt
    42061 gaacaggaag ccaccagaca gtttacccgc agtcgggtca cccgccatat aacgcggaat
    42121 gtcgccgtgg aaaacctgac ctgctgcgtt ggtgtattca ccaatctgca tctggaaagg
    42181 tacgttccag atgtggtgca gaccaaacgg taccagacaa cgttcgatga aaccgtaaat
    42241 gccaaacgca actaccgggt tctggtaagc agcccactga gagaaggtct ggattgcaga
    42301 accaatcggc ggccaaatga aggacagcac aacgccagta aagatggcag ccaggccaga
    42361 aatgatcggc acaaagcgtt taccggcaaa gaagccaaga tactcaggca gcttaatacg
    42421 gtagaaacgg ttaaacatgt acgctgcaat cgcaccggag ataatccctc cgagtacgcc
    42481 agtatccgcc aagtgtttag aggcgatttc ttcagcaggt aaatgcagta ccagtggcgc
    42541 aaccacggcc atggttttaa ccatgatgcc ataggcaaca actgcggcca gcgcggatac
    42601 gccatcgtta ttggtaaagc cgagggcgac accgatcgca aaaatcagtg gcatgtttgc
    42661 aaagacggaa ccgcctgctt ctgccataac atgcgataca acggcgggca gccagctgaa
    42721 attcgcggaa ccgacgccca gcagaatacc tgcgataggt agtacggata ccggcagcat
    42781 cagcgattta ccgacctttt gcaggttagc aaatgcattc ttaaacataa ttgagagtgc
    42841 tcctgagtat aggtgctttt tttacgttct cacgcgtggc aaggggggag agcctcgccg
    42901 tgtacagggc atctaagcgc cctttattta ttacacagag taaaataatt cagtgccaat
    42961 ttgtttgacg gctatcacgt ttcaacttca gatcattgga gaagttgcac attttgacaa
    43021 aacatatgtc acaatatttc aaacgcggtt attcaccgcc caattttcgg cggtgaactt
    43081 tactcgtttt agccattaat tacgagcttt aaaaaaactc attcaacgga tggattgaag
    43141 gcgggaagcg tcgatgtgaa acagacgggc gaagttatcg gtggttacct gcgccagttc
    43201 ttcaacggca acacctttca acacagccat gtattctgca acgtcacgaa ccatcgcagg
    43261 ttgattctct tttcctcgat gcggtaccgg cgcaaggtaa ggtgagtcag tttccaccag
    43321 taatcgatcc agggggacat aacgcgcagc atcgcacagt tgctccgcat tacggaaggt
    43381 cacaatgccg gaaaaggaga tgtaaaatcc gagatccagt aatttacccg ccgtttctct
    43441 gtcctctgta aaacagtgta gtacgccacc gcaatccgtc actttttctt cgcgcagaat
    43501 tgccagcgta tcggcacggg cgtcacgggt atggacgata accggcttgt tcagttcacg
    43561 accaatctgg atatgatgga tgaaggactc ttgctgacgt actttagttt ccggtgtgta
    43621 ataataatcc agcccggttt cacccagcgc tacaacaccc tcttctgccg ccagacggcg
    43681 taaatcttct acatcgtagg gatcattctg gttgagtgga tgcacgccac aagaaaatac
    43741 gacgttgtca cgttcgccta ccagatcccg catatgtaaa taacccggta atgttgtggc
    43801 gactgccaga caaaatttca catcgcgtgc ggcggctttc gccagaacgt catccacgtc
    43861 cttatgcaaa gattcataat ccagaccatc gagatggcag tgtgagtcga ctaaaaacat
    43921 gatgtctctc ttaaagatga ggaaccggta gcacaacgcc cggttgcagg taatgctcaa
    43981 tacgcagtaa aagatcggtg atgagaagct cgcggttgat gcctgtgaca gacattaact
    44041 gttcacgaat gtggcaaaca tcccccagta tagcctgcag gcgcgaggga gaaagatggt
    44101 ttgccagttc ggcgaccagg cccggcacat caacattggt caccagcgca gcgccatgat
    44161 ggcgttttag cgcatccatc agcaacgttg ccacccagtg taaacgcgcc ggagcttgtt
    44221 catgattaag ggccgccagc agcgaatacc agtcgcccga tggcacgcta tatgccaacg
    44281 cctgacacaa tgtttcacga gcctgccagt tatctccctg aaacaacgcc agtgccgcgc
    44341 caggcgaacc ggcgcttaag cgcaatgcgg caagtaatgc atcctgtgac attgtcactt
    44401 cgcgtgaaag ccaagtcacg gcgtactgtt ctggaggcgg cgcaaggtaa tgtaaccgac
    44461 aacgactacg taatgttgcc agtaaacgtt caggctcgcg ggtagccagg aaaaaccagg
    44521 tttctgctgg tggctcttca agcgttttca gcaatgcgtt agccgcggcg tcggtcagta
    44581 aggcagcatc ggttacccag acaactttcg caccacctag gcgtgcgtgc tcattcagct
    44641 tttcggtgac ctcacgtacc gcatcaatgc ccagcgtatt tttccctttt tcgggagcca
    44701 gggtgtagta atcgggatgc gtgccagcct gcatcaactg acatccacga cagtgaccgc
    44761 aacttttgtg gccctgcggt tgttggcaga gtaaataacg gctcagggcg tagattaaag
    44821 catcatcgcc catgcccggt aacgcctgaa tgagtagcgc atggtgacct cttccggcct
    44881 gatagctggc taccagtttt tcgaaatcag gtcgtaacca tggataccat ctcatgcgtc
    44941 caactccttc acccagtggg tcacggtagt gcggattgca tccatcacgg cctccagcgg
    45001 ctgggtggca tcaatggtat gaatactttt atcttgtgca gccagttcca gatagcgggc
    45061 gcgggtgcga ttaaagaaat cgaaagactc ttgctcaata cgatccagct cgccgcgcgc
    45121 acgcgcgcgt tttaagccaa cttccggggt aacatcgaga tagagcgtta agtcggggcg
    45181 aaaatccccg agaacagcat cacgcagtgt tgccagcata tgttggtcaa taccacgtcc
    45241 tccgccctga tacgcctgag tggagagatc gtggcgatcg ccaatcaccc aggtgccgtt
    45301 agccagcgca ggtttgatga ccgtttctac cagttgaacg cgcgcggcat aaaacatcag
    45361 aacttcggct ttatcggtaa tgacttcatc gcctaccgat ttgatatcca gcaccaggct
    45421 tcttaacttt tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca tgtcgcggat
    45481 acccagttgc tcgagcgtct caaccaccac attacgcgcg gtagttttgc ctgcgccttc
    45541 cagcccctca atgacgatat acttactgcg catttttttc cttaagcact ttcagataat
    45601 cctgcacaga cttgttatga ctggcaagat tggtattaaa cgtgtgacca cctttaccat
    45661 cggccacaaa atagagatac ggcgtttttg ccggatgcgc agcagccttc agcgaatccg
    45721 cccccggcgt agctatcgca cccggcggca aaccggtaat ggtataggtg ttatacgctg
    45781 tcggcgtttc caggtctgca cgagaaagtt tgccattata acgctctccc atcccgtaaa
    45841 tcacggttgg gtcggtctgc aggcgcatac caatgcgtaa acggttgata aataccgagg
    45901 caacctgatc gcgttcactg gcaacggcgg tttctttttc gataattgat gccatcgtca
    45961 ccagctgatt tttatcttta taaggcagac cgtccgcacg cccttcccag gcgctatcga
    46021 ccgctttcac cattttcttg tgagctcgct tgagtaacgc gacatcggtg gtattggcgg
    46081 tatacatcaa ggtgtctggc cagaaccaac cttcaatcca ctccgggttt tccagttcaa
    46141 gtgcctgcgc tacggtggcg tacttatcgt cactcagcgt gtgcttgata tacggagcct
    46201 cacgcaattg cttgaggtag tcgctcagac gcatcccttc taccagtcgc agcgggaact
    46261 gtgcttcttt accgctttcc aacaatttca gcatctcgcg cacggtcatc tgcggtgtaa
    46321 agcggtaagt cccggcttta aagtgagaaa gatccggttc gatacgcagc agccattgaa
    46381 aaacccgtgg gcgattgatg atcttatcag cataaagctg ttcaccgagc gccagacgtc
    46441 cggtccctgg cttcagggta aatatcgtct cttctttgat aagcaatttg ctgtcggcaa
    46501 gatggcgaac cttccagacg cccacaccag cggcgatacc cagtaccacc agcaataaca
    46561 agataatcaa taacactttt ttcatgacta attcgggcgc tcacaaagtg gggctaaata
    46621 ttcatataac gttgctgacg aaaaggtgac atcgccacag gcacgtacgg gcatcactgg
    46681 cattaacgca ttacaaataa ccatctcatc tgcctgcaac gcctcctcca gcgaggcttg
    46741 cacttcgaca agctgataag aggattgtgc cagtaaacgg atacagaatt gtcgcataat
    46801 gccgttaaca cctgcctgat ccagtcgcgg cgtataaact acattgccct tccgccagaa
    46861 caaattagcc gcacagcatt ccgtaaccca cccttcgctg tcaaggacca gcgcctcatc
    46921 agcgtttgtc tgctcaagat gagagcgaat caatacttgc tcaagccgat taagatgttt
    46981 aatacctgca agatgaggat tgcgccccag ccgcaccggg cttagcgcca acgtcatccc
    47041 ttcgttacgc aaacggtcgt aatgtgcagg ataagccgta acggagagaa tccgcgttgc
    47101 tggtccgctg ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga tcacgacttt
    47161 cagcacacca ttttgctgtt ctgctgccag cgttttcatc tcttgttcaa gctgaggcca
    47221 gaagtcacag gaaatcatca accgctgaca agcatcctgt agtcgctgga tatgcgccga
    47281 taacaaactg actttaccgt cgataactct ggcggtggta aaacaaccat caccaaactg
    47341 cgttgcccga tcgcttactg ccagcgattc ctgcttataa ccgttaatta agaacatacc
    47401 ggctccttat cagagaattg ttagtgtggc agcatgttca ctacggaaca agtcggaata
    47461 aaagctaaga aaaaaggccc gcaagcggac cttttatatg ggtggaaaat gacaacttag
    47521 atctttttaa agatcaaaga accattagtg ccaccgaagc cgaaggagtt acacagcgtg
    47581 tattccattc cgctgacctg acgtgcttca tgcggtacga aatccagatc gcaaccttca
    47641 tccgggttat ccaggttgat ggttggcgga acagcctgat cgcgcagcgc caggatggag
    47701 tagatagatt ctactgcacc cgccgcacct aacaggtgac cggtcataga tttcgtggag
    47761 cttaccaaca cacggcttgc agcttcaccg aagatggttt tcaccgcctg cgcttcagct
    47821 ttatcgccag ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg actcgcttca
    47881 atgcctgcat cacgcagagc atttgccatt gccagagctg cgcctgcgcc attttctggc
    47941 ggtgacgtca tatgataagc atcgctgctc ataccaaagc cgacgagttc agcgtaaatt
    48001 ttcgcaccgc gttttttcgc gtgttcgtac tcttcaagta ccagcatacc ggcaccatcg
    48061 cccagtacga agccatcacg ctctttatcc cacgggcggc tcgccgcttg cgggttatca
    48121 ttgcgggtag ataatgcacg tgccgcgcca aaaccaccaa cgcccagcgg cgtactggct
    48181 ttctctgcgc cacctgcaac catcacgtca gcatcgccat acgcgataat acgcgcagca
    48241 tggccaatgt tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct tgggccacgc
    48301 aggccataca tgatagtcag atgacctgcc accatgttca caatcgttga cggaacgaag
    48361 aatgggctga tcttacgtgg accaccgttc atcagagatg tgtggttttc ttcgatcagt
    48421 ccgaggccgc caatcccgga gccaattgcg gcaccaatgc gggttgcgtt ctcttccgtt
    48481 atttcaaggc cagaatcctg catggcctga acgccagcga caattccata ttgaatgaag
    48541 gcatccatct tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa atcctttact
    48601 aagccagcaa atttcgttgc ataggcgcta gtatcgaaat ggtcgattag gctgatgcca
    48661 ctctgaccgg caagcagagc tttccaggta gactctacgg tattgccgac aggagacaac
    48721 atgcccagtc cggtcacaac tacacgacgc ttagacacgt ttgtcctcca aggagggaaa
    48781 aatgattcta gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc ctggagatgt
    48841 tcacttacgc ctggtggccg ttgatgtaat caatggcagc ctgaacggtg gtgattttct
    48901 cagcttcttc gtccggaatc tcagtatcaa actcttcttc cagagccatt accagctcaa
    48961 cggtgtcaag agaatccgcg cccaggtctt caacgaaaga agcattgttg gtaacttctt
    49021 cctgcttaac gcccagctgt tcgccgataa ttttcttaac gcgttcttcg atagtgctca
    49081 tactcttaaa tttcctatca aaactcgctt tcgcgatggt tttcgtagtg tataaaatgt
    49141 tgaaaaattt gcaactaaat cccggcaggt cttaccacga ttttacgtta ttttgaggcc
    49201 ttacccccca ataacgcaaa tcattttgca ctaattatta ctcataacca cgcagactgc
    49261 gcgcaacatg agcattttgt gcaaatcgcg gtcagaccat gtacatcccg ccgttcacat
    49321 gcaaagtttc acccgtgatg taagctgctt cgtcggatgc caggaatgca accgcgttgg
    49381 cgatttcctg tgcgccgccg aggcgacccg caggaacctg cgccaggata cccgcacgct
    49441 ggtcatcgct cagcgcacgt gtcatgtccg tttcaataaa gcccggagca acaacgttta
    49501 cagtaatacc gcgtgacgca acttcgcgcg ccagtgattt actgaagccg atcaagcccg
    49561 ctttcgccgc agcgtagttg gcctgaccgc catttcccat ggtaccaacc acagaaccga
    49621 tagtgataat acgaccatga cgctttttca tcatagcgcg cattaccgct tttgacagac
    49681 ggaaaacaga tgaaaggtta gtttcgataa tatcgttcca ctcttcatct ttcattcgca
    49741 ttaacaggtt atcacgagtg ataccggcat tattgaccag gatatccact tcaccaaatt
    49801 ctgcgcgaat tttttccaga acagattcga tagatgccgg gtcggtcaca ttcaacatca
    49861 gacctttgcc gttggcacct aaataatcac tgatcgcctg agagccattt tcactggtcg
    49921 cagtgccaat aactttcgcg ccacgggctg cgagcgtttc agcaattgcg cggccaattc
    49981 cgcggcttgc accggttacc agtgcgattt ttccttcaaa attcatgatt ttcctctttt
    50041 aaagctcgag cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg gtcagggtgt
    50101 cgacaatgcg tttcgtcagg ccagtaagca ccttgcccgg gccgacttca tagagatgtt
    50161 ctacgccttg cgctgccatg tactcaacag acttcgtcca ctgaaccggg ttatacaact
    50221 gacgtaccag tgcgtcacgg atggcatcac cattggtttc gcatttcaca tcaacgttat
    50281 tcacaacaga aactgttggt gcgttaaagg tgattttcgc taattctact gccagtttgt
    50341 cggctgctgg tttcatcagc gcacagtgag acggtacgct cactggtaac ggcagcgcgc
    50401 gttttgcgcc agcagcttta caggcagcgc cagcacgctc aaccgcttct ttatgaccgg
    50461 caataaccac ctgtcccgga gagttaaagt ttaccggaga aacgacctga ccttctgcag
    50521 cttcttcaca cgctttcgca atagacgcat catccagacc gatgattgcc gccatagcgc
    50581 ccgtgccttc cggtacggct tcttgcatga acttaccgcg catctcaacc aaacgcaccg
    50641 catcagcgaa atcaatcacg ccagcgcaaa ccagcgcgga gtattccccc aggctgtgac
    50701 cggccatcat tgccggtgct ttaccgccct gctgctgcca tacgcgatac agcgcaacag
    50761 atgcagtcaa cagcgctggc tgggtttgcc aggttttatt cagttcttca gctggcccct
    50821 gctgggtcag cgcccacagg tcgtagccca gcgccgcaga agcttcagca aacgtttctt
    50881 cgacaattgg atagctcgcc gccatatcag ccagcattcc aacggtttga gaaccctgtc
    50941 cagggaacac aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa cgaaccagcg
    51001 cggagcccca agtgaatcca ccgccaaagg cttcaagcag aaccaactgc cccggcttaa
    51061 tgcgcccgtc gcgtacagct tcatccagcg cgcacgggac agaggccgca gaggtattac
    51121 cgtggcgatc cagcgtcacc acgacattgt ccatcgacat accgagtttt ttcgccgttg
    51181 cactgataat acgcaggtta gcctgatgcg gaaccagcca gtccagttga gaacggtcaa
    51241 gattattcgc cgccagcgtc tcatcaacga tgtgcgccag ttccgttacc gcaaccttga
    51301 agacttcgtt gcccgccatc gtcagatgaa ttgaattctc tggattcacg cggtcggcgt
    51361 ttggcagcgt cagcaattca ccataactac cgtcggcatg cagatgggtg gaaatgattc
    51421 ccggctcttc agaggcagcc agcaccgcag cgcccgcgcc atcgccaaaa ataataatag
    51481 tcccacgatc tgttggatcg caggtgcgcg ccagtacatc ggaaccgacg accagagcat
    51541 acttcaccgc cccagatttc acgtattgat cggctacgct taatgcatag gtgaaacctg
    51601 cgcaggctgc tgcaacgtca aatgccgggc aacctttaat gcccaacatg ctttgaatct
    51661 gacaagctgc gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg atcaggccaa
    51721 tctggtcttt ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca aagcccatgg
    51781 ttgaaacggt ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta cgagtgacaa
    51841 tccactcgtc agaggtgtcc accatttttt ccaaatcggc gtttgtccgc acttgttcgg
    51901 gcagatagct gccagtacca ataatcttcg tatacatgta cgctcagtca cttttcggtt
    51961 atataccgtc acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg cagagttccg
    52021 cttttgccac cgtccagcag ctcaaaacca gctgggtata cagattccag gcgagcggca
    52081 attcgctgag gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc gaccgcaaaa
    52141 gctcgctgat tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc taacagacag
    52201 gcgccgttat actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct cttttgtagc
    52261 caacgcttta ataacagtag ccaccacgac cgttttttcc cttcaccctg agatttcagc
    52321 agagaaagga acatcctgac aacaccttcc atcgtcttta acgtgacatt tcctgtaaag
    52381 ccgtcacaaa ccagcacatc tgtcttgcca gttaacaact cattggcttc aagatagccg
    52441 atataattga tagaagggat tgttttaagc accgctgagg catcccgaat actgtcaaga
    52501 ccctttactt cttcttcacc aatattgagc aacgccacgc gaggattggg aatttccacc
    52561 acctcttcag ccagaactga gcccataatg gcaaattgca ccaacattgt gctgtcacaa
    52621 tcgacgttgg cccctaaatc gaggaccacc gttttgccct tttgctgatg tggtaatacc
    52681 gtcaccagcg ccggacgctc aatcccctcc aggggcttga gtaataattt tgccagcccc
    52741 atcagtgccc cggtattacc ggcactgaca caggcttgcg ctcgaccttc tttcaccagc
    52801 tccagggcca tacgcattga actcccacga ctggcgcgga tagcttgcga aggccgggca
    52861 tcactggcga taactgactg cgcaggaata atctgcagac gcgaacgttg ttcaaagtca
    52921 gctttagcaa gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag agtgagttgc
    52981 gaattagaat tcagtgcctg caatgctgca ggcactgtca cggaagggcc aaaatcccct
    53041 cccatgacat ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc cggtttggtc
    53101 tttccccagt tgcctgggga aaatcctcac taagcttcat cacgcagacg cgtgattact
    53161 tagcgatgac cttgcggccg cggtagtaac cgtcggcagt gatgtggtga cgcaggtgtt
    53221 tttcaccaga agttttgtct acagacaggc tggtgactgc ggtcagcgcg tcatgggaac
    53281 gacgcatgcc acgtttggaa cgggttggtt tattctgttg tacggccatg gaccttactc
    53341 ctcaattacg gtttatcccc gatccaacgg ggagcaccaa ttacttacgc tttaagctgg
    53401 ctaatacggc aaatgggttt ggcttttgca cttcttcagg cagttcacca aagaccatat
    53461 ccgcttcgga cacttcacag tgttcagaat catgcaccgg aactaccggc aaggcgagga
    53521 tgatttcatc ttcaaccatt gcaagcagat cgatttcacc gaattcgtta acctcaatcg
    53581 gttcatacgc ttccggcagt gcttcagcct gttcgtctga acgcacagga ctaaaacaat
    53641 acgttgtgta gacctgatga gtaaacggct tcccgcaacg ctgacactcg agcgttaccg
    53701 tcaccttcgc atcgccgttt aacactgcga gacgttggtt atcgatagcg aacgacatgg
    53761 agcattccac atcactgtcc acactgacta cggattcggc gacgcgctca acctgatcag
    53821 gggtatagat accctggtaa tcaaggcgtt tttgagccgt acgaaccgga tcgagagtca
    53881 gggggaattt taccttttgc atagggcgcg catattaact ttgtaacgtc atagagtcaa
    53941 agaaaaaggc agcctgtggt tgccttttgc caataattcg cacacattgc gggttactgc
    54001 tttattttcg ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg catagtttaa
    54061 aatggctatc atcaatacgc tatatctgat ggaaaaaaat atgcctaaac ttattttagc
    54121 ctccacctcg ccatggcgtc gcgctcttct ggaaaaactg caaatctctt ttgaatgtgc
    54181 agcacctgag gtcgacgaaa ccccacgcag cgacgaatca ccgcgacagt tggtgcttcg
    54241 actggcacaa gaaaaagcgc aatctctggc gtcacgttat ccggatcatt taattattgg
    54301 ttcagaccag gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa cggaagaaaa
    54361 tgcccgtctg caattacgca aagccagcgg caatatcgtc accttctata ctggactggc
    54421 gttgtttaat tcggcgaatg ggcatctaca aacagaagtg gagccttttg acgtccattt
    54481 ccgtcaccta agcgaggcgg agattgataa ttacgtgcgt aaggagcatc ccctgcactg
    54541 cgcgggtagc tttaagagtg aaggatttgg cattacgctg tttgagcgtt tagagggacg
    54601 tgaccctaac acgctggttg gtttaccgct aatcgcgctg tgtcagatgt tacgtcggga
    54661 agggaaaaac ccgctgatgg gatagttgtt tgcgccggat gcggttacgt cgcatccggc
    54721 aatcggtgca ggcggcctga tgcggcgtaa acgccttatc aggccaaccg cccattatca
    54781 ggcttatatg cttgtattag cgcgcgttac gcagtttttg caaacaacgc ttcaaacctt
    54841 catccatcgg cgcttcgata cgcatcacct caccggtccc cggatgggta aacttcaacg
    54901 ctgcggcgtg caggaacaga cgatttaatc ccgtgcctgc ttcagtgagc tgtctgtcaa
    54961 attcacggtc accgtagcga tcgtcaaagg caatcggatg acccgcatac tgtgtatgca
    55021 cacggatctg atgagtacgt ccggttaccg ggctacaacg caccagggtg gcaaatgcat
    55081 agcgttcttc cactttaaag cgtgtttccg acggtttgcc ttcctgactg acacgcacga
    55141 tacgttcgcc gctttgcaga atatttttca ataacggcgc ttgaacgctc ttcacatgcg
    55201 actgccactg accgcgcacc agcgccaggt aatctttttg cattcctttt tcacgtaatt
    55261 gctcatgcag agaacgcaac gccgagcgtt ttttcgctac cagcaacaca cctgaagtgt
    55321 cccggtcgag acgatgaacc agttcaagga accgcgcttc cgggcgcaac gcccgcaaac
    55381 cttcgataac gccgaagctt aaaccactgc cgccatgtac cgccgtaccg gaaggtttat
    55441 tcagcaccag gatatgatca tcttcatata agatgacgtc cgccagcgcc gccacctttt
    55501 gcagatgtgg cgaaaccgcc tcttcttccc gctcagcaac gcgaaccggt gggatacgca
    55561 cctcatcacc cgcttcgagt ttatattcag gcttaatacg ttttttgttc acccgcactt
    55621 cacctttacg caaaatacgg taaatcatac tttttggtac gcctttcaat tgggtacgca
    55681 aaaagttatc gatacgttgc cccgcttcgt cagcggtgat agcaacaatt tttacggatg
    55741 gagtctctgt tttcatggtg ggcgattcta attagccaac aggattcgcg ccactcattt
    55801 ttctatgctt atatttagtt ttgcacctta ttacttcact acgtgatcac tttattgatg
    55861 gttattaaac caatcaccag caagaagtga aaaaactgtg agtaagcggg tgataaatgg
    55921 taaaagtcat cttgctataa caaggcttgc agtggaataa tgaggccgtt tccgtgtcca
    55981 tccttgttaa aacaagaaat tttacggaat aacccatttt gcccgaccga tcatccacgc
    56041 agcaatggcg taagacgtat tgatctttca ggcagttagc gggctgcggg ttgcagtcct
    56101 taccggtaga tggaaatatt tctggagagt aatacccagt ctgtttcttt gataattgcg
    56161 ctgtttttcc gcatgaaaaa cgggcaaccg acactctgcg cctctttgag ctgacgataa
    56221 ccgtgaggtt ggcgacgcga ctagacacga ggccatcggt tcacacccgg aaaggcgtta
    56281 ctttgcccgc agcttagtcg tcaatgtaag aataatgagt aagttacgat gaaaagaatg
    56341 ttaatcaacg caactcagca ggaagagttg cgcgttgccc ttgtagatgg gcagcgtctg
    56401 tatgacctag atatcgaaag tccagggcac gagcagaaaa aggcaaacat ctacaaaggt
    56461 aaaatcaccc gcattgaacc gagtctggaa gctgcttttg ttgattacgg cgctgaacgt
    56521 cacggtttcc tcccactaaa agaaattgcc cgcgaatatt tccctgctaa ctacagtgct
    56581 catggtcgtc ccaacattaa agatgtgttg cgtgaaggtc aggaagtcat tgttcagatc
    56641 gataaagaag agcgcggcaa caaaggcgcg gcattaacca cctttatcag tctggcgggt
    56701 agctatctgg ttctgatgcc aaataacccg cgcgcaggtg gcatttctcg ccgtatcgaa
    56761 ggcgatgacc gtaccgaatt aaaagaagca ctggcaagcc ttgaactgcc ggaaggcatg
    56821 gggcttatcg tgcgcaccgc tggcgtaggc aaatctgctg aggcgctgca atgggattta
    56881 agcttccgcc tgaaacactg ggaagccatc aaaaaagccg ctgaaagccg cccggctccg
    56941 ttcctgattc atcaggagag caacgtaatc gttcgcgcct tccgcgatta tttacgtcag
    57001 gatatcggcg aaatccttat cgataacccg aaagtcctcg aactggcacg tcagcatatc
    57061 gctgcattag gtcgcccgga tttcagcagc aaaatcaaac tgtacaccgg cgagatcccg
    57121 ctgttcagcc actaccagat cgagtcgcag atcgagtccg ccttccagcg tgaagtccgt
    57181 ctgccatccg gtggttccat tgttatcgac agcaccgaag cgttaacagc catcgacatc
    57241 aactccgcac gcgcgacccg cggcggcgat atcgaagaaa ctgcgtttaa caccaacctc
    57301 gaagccgccg atgagattgc tcgtcagctg cgcctgcgtg acctcggcgg cctgattgtt
    57361 atcgacttca tcgacatgac gccagtacgc caccagcgtg cggtagaaaa ccgtctgcgt
    57421 gaagcggtgc gtcaggaccg tgcgcgtatt caaatcagcc atatttctcg ctttggcctg
    57481 ctggaaatgt cccgccagcg cctgagccca tcactgggtg aatccagcca tcacgtctgc
    57541 ccgcgctgct ccggtaccgg taccgtgcgt gacaacgaat cgctgtcgct ctctattctg
    57601 cgtttgatcg aagaagaagc gctgaaagag aacacccagg aagttcacgc cattgttcct
    57661 gtgccaatcg cttcttatct gctgaatgaa aaacgttctg cggtgaatgc gattgaaacg
    57721 cgtcaggacg gtgttcgctg cgtgattgtg ccaaacgatc agatggaaac cccgcactac
    57781 cacgtgttgc gcgtgcgcaa aggggaagaa acgccgacct taagctacat gctgccgaag
    57841 ctgcatgaag aagcgatggc gctgccgtct gaagaagagt tcgctgaacg taagcgtcct
    57901 gaacaacctg cactggcaac ctttgccatg ccggatgtgc cgccagcgcc aaccccagct
    57961 gaacctgccg cgactgtcgt agccccagca ccgaaagctg caacggcaac accagcagct
    58021 cctgcacaac ctgggctgtt aagccgcttc ttcggcgcac tgaaagcgct gttcagcggt
    58081 ggtgaagaag ccaaaccgac cgagcaacca acaccgaaag cagaagcgaa accggaacgt
    58141 caacaggatc gtcgcaaacc tcgtcagagc aaccgccgtg accgtaatga gcgccgcgac
    58201 acccgtagtg aacgtactga aggcagcgat aatcgcgaag aaaaccgtcg taatcgtcgc
    58261 caggcacagc agcaaactgc cgagacgcgt gagagccgtc agcaggctga ggtaacggaa
    58321 aaagcacgta ccaccgacga gcagcaagcg ccgcgtcgtg aacgtagccg ccgccgtaac
    58381 gatgataaac gtcaggcgca acaagaagcg aaggcgctga atgttgaaga gcaatctgtt
    58441 caggaaaccg aacaggaaga acgtgtacgt ccggttcagc cgcgtcgtaa acagcgtcag
    58501 ctcaatcaga aagtgcgtta cgagcaaagc gtagccgaag aagcggtagt cgcaccggtg
    58561 gttgaagaaa ctgccgctgc cgaaccaatt gttcaggaag ctccagctcc acgcacagaa
    58621 ctggtgaaag tcccgctgcc agtcgtagcg caaactgcac cagaacagca agaagagaac
    58681 aatgctgata accgtgacaa cggtggcatg ccgcgtcgtt ctcgccgctc gcctcgtcac
    58741 ctgcgcgtga gtggtcagcg tcgtcgtcgc tatcgtgacg agcgttatcc aacccagtcg
    58801 ccaatgccgt tgaccgtagc gtgcgcgtct ccggaactgg cctctggcaa agtctggatc
    58861 cgctatccaa ttgtacgtcc gcaagatgta caagttgaag agcagcgcga acaggaagga
    58921 gtacaagtgc agccgatggt gactgaggtc cctgtcgccg ccgctgtcga acctgttgtt
    58981 agcgcgccag ttgttgaaga aatggctgaa gtcgtagaag cccccgtacc ggttgccgaa
    59041 ccgcaaccgg aagtggttga aacgacgcat cctgaagtga ttgccgccgc ggtaactgaa
    59101 cagccgcagg tgattaccga gtctgatgtt gccgtagccc aggaagtcgc agaacacgca
    59161 gaaccggtgg ttgaaccgca ggaagagacg gcagatattg aagaagttgc cgaaactgct
    59221 gaggttgtgg ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt cgtcgctgaa
    59281 gtcgcagcag aagttgaaac ggtagccgcg gttgaaccag agatcaccgt tgagcataac
    59341 cacgctaccg cgccaatgac gcgcgctccg gcaccggaat atgttccgga ggcaccgcgt
    59401 cacagtgact ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc aggtggtcat
    59461 acggcaacgc atcatgcctc tgccgctcct gcgcgtccgc aacctgttga gtaataatta
    59521 gctcaaagta atcaagccct ggtaactgcc agggcttttt tatttcatgt ttgaatcaac
    59581 atattaaaat tcatcaaatg gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaaa
    59641 tgcccgcccc ggcgatccca gagcgggcag acaaaacata cccagtttca tgatatgttt
    59701 taaagcgagc ttatttgttg agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt
    59761 tatacgatgc ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt gcattccagt
    59821 ccacatcaac cagatcgctc atctgctgcg tttgccctaa agcgcgatcg ctacctaagg
    59881 aatccagcga ctccagtttg ttcagctgcg tgcctaattc cgtgcgaacg gtaaggacat
    59941 tattcagcga gtttttcagt ccacggttgg ttttatccaa tgccgcagcg gcgatttctt
    60001 tatccgcttc gctatccgcg accggcgttt tcagggctgc gatggcgcta tccagcatgg
    60061 caaaaagatt ggtttcagaa gcgctaccgt ctggttccgc taccgcgtta ctggtaatac
    60121 tgtcgaaaat tttgtcgccc gtatgtccta tcaccatcga acgcgaagca tcgacctgtt
    60181 gcttaatact ttctgttccg ccgacataat caccgttagc ctcactaaac ggcgcagtct
    60241 ctgttttata accggcaaaa atgtagcgcc cattaccgtc agtggtgttc gccagattca
    60301 gcaactggtc acgaagcccc tgaatatccg tagccagcga ggcccggtca tcgtcactca
    60361 aggtgccatt gctggcgtag acaatttttt cctgagcatt ctggatagca gtggtgacct
    60421 ggctaagtac actctcttcc agtgacactt tttgagtggc gaaagtacgt gccagcgtgt
    60481 actggctgtt ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca gcaatgggat
    60541 cgtctgaagg gttaacgact cgcttacccg tcgacatctg ctcgccgtac ttcatccatt
    60601 ctgcctgaga attggtgatg ccgcgcatgt tttgctggta catcatctgg gtactgaaac
    60661 gcattttatc tccccttagc gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg
    60721 cagaacctgc gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc cgtattcctc
    60781 atcgagattg acaccggaaa tcgactgctg ctgattggaa agctgcgtca ccacattacc
    60841 ttgcgtggtg ctactggttt tcaacgtcgc ggttttatta ccgatatcac tcactaacga
    60901 ggcataagcg tcgttaaagg atttcgcacc gcccaccgtt ttactgttgc tttgcagatc
    60961 cagcagggcc tgaccgttgc ggttgtcgct atcacccgca tcttcttcgc tcgccatcgc
    61021 gattttcgct tcgtcggtga ttaatacatc catgttgacg atggcgtcac ttactggttt
    61081 cagcgtgaag ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa
    61141 tgccacttta ccgttggcat ctggtgtcgc cgtaaaagtg gtattgctgg caaggcgggt
    61201 gacctgccac tgattattat cgaacgagat tttgtaatct gtcgccagta ccacggaggc
    61261 atcagttacc gtggcaccga tcgcaacgtc acctttgttt ttcgtgtttt gctgaaccgc
    61321 gggattaccg atagtaaaga aatcttcacc agcatcgccg ttagcatcaa atccggcttt
    61381 gtgttggctg ttgaaagcct cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt
    61441 ctggtccaga tcctgagaac ggaatgtcag aatgccgccc agcgacccgg tattcagtaa
    61501 tttctccggg atctcaatat tgcctgccgt cccatcaacg taagcgacag tcgtacgaga
    61561 agggtcggcg ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct gaaccagtga
    61621 gtaaccattg gcatcgtgat gttataagta ccgccatcct gaacgctgac ttctacgcca
    61681 acaatctggt ttaattcgct caccagttga tcgcgttgat ccagcagatt gttaggtgac
    61741 gcccctgccc ccacgcctgt caggcgcgag atttgatcgt tcaggctggc aatttgttta
    61801 gcgtaattgt tgatctgatc aacgctggca cctatcgcga tattgacctg tttgtcctgg
    61861 tcgcgcagat attgatcggt ggttttaaac tgattcacca acccttctga tttcccaatc
    61921 agcgcctggc gcgccgccgg gtcttccgcg ttactcacca gcgtttgcag gctggtgaag
    61981 aaatcctgca tctgtgttgc cagcgaagag gtactggtgg agagcatatt gtcgattttc
    62041 gacatctgct catagcgggc ggtcaggccg ctactttgcg tctgcacagc acgtaactgg
    62101 ttggtaataa acgtatcata ctcacgctgc acaccagaaa cgtagacgcc attgccaacc
    62161 cagccgccag cgcccaacgt gctattggcc tgcgccataa tagtggtttg gcgggtatat
    62221 ccggcaacgt tatagctgga gatattatta cttgccgtat ttaacgccgc ctgggccgcg
    62281 ttcagtccgc tcatggcgtt attgatcaag ctggacatgg aggttcctta taagccttca
    62341 attacagagt attatcggca gcgacccgcc ggacttgagt tattcagaac agattatcaa
    62401 tgttcatgct gtaggttttg ctcacctggt cgcttatcga tttcatctgc tggatcatgt
    62461 tggtgagttt acgggcatag tgaggatcgg tagcatagcc cgcgtcctgt aggacctgcg
    62521 ccccctgttc cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta cgtgttaaca
    62581 gcccaacgta atccgacaat gcttccagat acgagctgta gacccgaaac ttcgctttta
    62641 ctttcttcgc ttcgccgttt tcatattcag tcgtggtgat ttcagtgact tgccctttcc
    62701 agttgccaga ggctttgaca ccaaacaggt tatagcttgg ctcgccgttt tcgcggcgga
    62761 tttgccgttg cccccaacca gattcaagcg cagcctgagc gaggatcaaa tgatgtggca
    62821 caccgctttg ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg aatgctctac
    62881 tgtcacccgg cagcgaatca tcgtagttac gtggtacggc cttttgcacc agctgcgaaa
    62941 gcgtctggtt ttgataacgc accacggttt cgagcgggaa tttcatcggt gctgctggcg
    63001 tggactcctc tggcaatggt tgttctggcg tcatctgttt aaccatcatc tctgcaagcc
    63061 ccagaccttt gcccgccgtc atctgttggg caatttgctg gtcatacata ctggtataca
    63121 gacgagtgtg ctcgctgctg aacaggccat cttttggtaa agcgtcgcgc atgcttttca
    63181 acatcatctg cacgaacatc ccttccacct gacgggccac cggacggata tttgccgccg
    63241 gatcttcgct cgctttcgcc tttagttcgt tgagtgattg cgcatcccag gccgcacttg
    63301 ccagtagttt gctgtcgctg atcatcagat gatttccagt tttgcccgca gacatcccgc
    63361 actttgcatt gattgcaaaa tagacatcag atccatcggc gtagcgccca gcgcattgag
    63421 cgcacgcacc acgttattga ggctggcgct ggaacgtacg ctttgcagcg aaccgccgct
    63481 ctggcgtaaa tcgatctgcg tttgtggagt aacaacagtc tgtccaccac caaacggtgt
    63541 atctggctgg ctgacattgg cctgacgatt aactgttact gagagatttc cctgcgctac
    63601 cgcgcagctg tcgagggtca cttctcgatt catcaccacc gaaccggtgc gcgagttaat
    63661 cactacttta gcgtcctgcg gggtgacatt aacctgcata ttctggatat cggcaaggaa
    63721 acggacctgg gaactgttgc cacttggtac gcgcacctga atagtccgcg catctaacgc
    63781 ggtggcgctg ccatatccac gcacgcggtt gatggtgtca gcgatttgct gcgccatgct
    63841 gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg acgccaaact ggctgggcaa
    63901 ttcacgttca ataaccgcac cattggtgat ccgtccaccg ttcagttggt taacctgaac
    63961 actgctaccg cccgcggagg ctcctgcgcc gccaaccaga atattgccct gcgccagcgc
    64021 atacacctga ctgtcaacgc ccttaagcgg tgtcatcaac aacgtacctc cacgcaagct
    64081 tttggcatta cccatggaag aaaccaccac atcgatggtt tgcccctgac gtccaaacgg
    64141 aggaagtgac actgtcacca ttaccacagc gacgtttttt agctgcatat tggtgcccgt
    64201 cggaacggta attcccagct gtgagagcat gttattaagc gtttgtgtgg taaacggcgt
    64261 ctgggttgtc tggtcaccag tgccatccag ccccactacc aggccatagc caatcagtga
    64321 gttttgcctt accccctgaa cactggtgag atcgcgaata cgctcagcct gagccgccgt
    64381 cgtaaccagt agaagaatta atgcagagag aaatttaatc accacagcct cacttacatt
    64441 ggcgacaggt taaggaagaa acgctgcaac cagcccatat tttgcgcttc gttaatgtag
    64501 ccattgccta cgtattcaat acgcgcatcc gccacctgag tagacggtac ggtattgctg
    64561 ccgctgatag tgcgtggatt aaccacgccc gagaagcgaa taaattcggt accctgatta
    64621 atggcaatct gtttttcacc caccacatgc aggttgccgt tgaccagtac ctggtcaacc
    64681 gtcaccgtca acgtgccgct aaaggtattg ctggcattgg ccccgccctt tccgttgaac
    64741 gtgttaccac cggaggcttc gacatcggca cgagcgttac caaacagccc ctgcaaatag
    64801 cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt cgcagaggag
    64861 cttttgctgg cgctgacgtt ctcctgcaac acgatggtca gcgtatcgcc aatattgcgt
    64921 ggtcgacgat cttcaaacag cggttgatag ccatagttaa tcggctgagc agactggaaa
    64981 atagaaccgt tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt cgccccctgc
    65041 accagcggcg tggagggtat ccaggcgcag ccggttagtg aaagcaccaa caagctggaa
    65101 atggcataag tatgcgcagc gtttttttgc atggctatca tcttcaaaaa tcagtaaacc
    65161 ggtgaacctg ccaccggtta tgccttagag ttgcgtcagt ttttgcagca tctgatcggt
    65221 ggtggacacc gctttactgt tgatttcgta agcgcgttgc acctgaatca tattgaccag
    65281 ttcttccgcc acgttgacgt tagacgtttc aacataccct tgatacagca gtcccgcgcc
    65341 gttcaggccc ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg tgtagaggtt
    65401 ttcgccaatg ctctccagtc cggtatcatt cataaaggtg gtgagattga gctgcccaac
    65461 ctgaaccgga gctgcctggc cttgttgggt tacgctgacc acgccatcac gaccgatggt
    65521 gatacttaac gcattcgccg gaatggtgat cgctggctgc acctgaaaac caccagccgt
    65581 caccagctgc ccgttctgat ccacctggaa agagccgtca cgggtatagg ctgatgagcc
    65641 atctggcaac attacctgga aaaagccctg ccctttaatc gcgacatctt tgctgttatt
    65701 ggtctgcgac agattgccct ggctgtgtaa gcgttcagtg gcgaccgggc gtacccccgt
    65761 gccgatttgt aatccggagg gtaaggtggt ttgttcggaa gactgtgccc ccggctggcg
    65821 aatggtttga taaagcagat cttcaaacac cgcgcgctga cgcttaaaac cgttagtact
    65881 gacgtttgcc aggttgttgg caatgacgtc catattggtt tgctgggcgt caaggcccgt
    65941 tttggcgatc cataatgaac tgatcatata atgtcctgta gtggattgct gacaagcaga
    66001 gcagaagcac gcgacagtgt gaatttcccc tcaccctaac cctctcccca aaggggcgag
    66061 ggaatagtcc gcgcgctttt attgcaatgt catcaatcgt tcaattaact catcgacagc
    66121 agttggttgg cacggcctgc gttatcatcg acgctgctga tcaccttcat ctgcatttca
    66181 aaacgccgcg cgctggcaat catgtcgctc attgccgcaa cggcattgac gttactgcct
    66241 tccagaaccc ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg ccctcgcgtg
    66301 gcctgggttt ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac ttcgctgccc
    66361 gtggctttca ccagtttcag acgccctact ggcgcaactg tatttgccgg atcgcccgga
    66421 ttgagcgccg agattgtgcc atcggcagca atcgtgattt ccgccccttc cggcacagca
    66481 attggccccg cctcgcctat caccggatgc ccctgaattg tcagttgccc ggtgggatca
    66541 acctgaatgc tgccattacg cgtataccct tcgctgccgt cagcggtctg cacggccagc
    66601 catcctgctg caacgcaacg tccagcgggc gcgaggtgta atccatcttg ccgggcgtca
    66661 tgtctgcacc cggtgttgac gccgttacca gcgtgcgcgt gggcagtgaa agcccttcca
    66721 ccggcactgc gcgcaaggcg tttaactgcg cgcgaaaacc gggcgtagag gcattggcca
    66781 gattactggc ggttaccgcc tgttgattca gcgtctggct ggctgctccc atcgcagtat
    66841 aaattgcgtg atccattgag ctatcccgtc agcgattagc gtaagttaac ccgcgtgttg
    66901 aggatctggt cctgggtttt gatggtctgg gcgttagact gatagttacg ctgggcaacg
    66961 atcatattga ccagttcttt actgagatcg acgttggacg cttccagcgc accgttggtc
    67021 agggtgccaa agtttcccgt cccggctgtc cccaacagcg ccacgccaga agattgcgtc
    67081 gcagaccaga cgttgtcgcc ttcggatgcc agaccttcgt tgttggcaaa gttcgccagt
    67141 acaatctgcc ccagcagttg ggtttgttcg ttggaatagt tgccgacaac cgtaccgtca
    67201 tcattgattt gataactcac cagatcgccc ggtttgtagc cattctgggt ggttgccaca
    67261 atgttgttag cgccggtatt ttgctgcatg gagttgagga agctcagact aaacgtggcg
    67321 ggttctgcac cgttaattgc gccggttgcg atattattcg ccatcgcaca tgtgccatta
    67381 gcattaaatt ccagtgttgt cgctgtcttc gcaatgctgt ttggatcact gctatcctgg
    67441 gtgtagacct gccagttatt atccccggtc ttcacaaaat agacgctcat gtcatgagca
    67501 ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc cgcattgctg
    67561 gcgctgaatg gcgtaacagt aggaagcgga tcactggaat tcaggttgat ctgcatcgac
    67621 gccgtggtgg tagttttcgc tgccatcagg gtattcggga tcgaaatatt ggtcggattc
    67681 gccccttgct gaatagtcgg cggcgtaccg gttgccgggt aacccgtcag ctgtaaacct
    67741 tgcatattca acaggttacg gttttcatcc agcttaaatt gtccgttacg gctgtagaac
    67801 accgaaccgt tgctgtctac cagacggaaa aaaccgttct ggctgatagc aacgtccaga
    67861 cctcgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc ggcaactttt
    67921 acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt gcctgattta
    67981 aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt ggtggcggca
    68041 gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa tgtgaaggct
    68101 taaattatct gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag atccagcgta
    68161 ttaccgctgt tgccgcggat cacaccctgc accagagcaa actgcagcgg ctgggcaacc
    68221 agttgtgtac caccgttact ggcgctaatc gctacattgt aagaaccgtt cggcgcagtg
    68281 ctgccatcag tcaacgtacc gtcccaggtg aaactgtgaa ctccggcggt cagttcacca
    68341 atatcaatgg tgcgcacaac cgcgccattt ttatcggtga tggtggccgt aactttgtct
    68401 gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg tcacagcccc ttcttcactg
    68461 ccggttcccg caagaacagt tgtgccgggg atcatcacgc cgtgaccgat caggttactg
    68521 gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt ggtattgagt
    68581 ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg acgtcagctc gttgttttcc
    68641 attggattgg tcgggtccta gtttttcagc tgcgccacca gcaaagtcag aaaactgctt
    68701 tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact gacgccggta
    68761 tttgtcggat cggtggtggt taccgcaatg gacatagctt tctcctttat tgaccgagcg
    68821 taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg gcctgatagc
    68881 tgcgtgacgc cgacatggtg ttaaccatct ccccgacgac atcaacgttc ggcattttta
    68941 cgtagccctt tgcatctgcc agcggattac ctggttcata aaccagtttg tccggggcct
    69001 gactttctta acatcggcaa cctttacgcc gcctgtcgca gcacctggtg cagcgttaac
    69061 ctggaatacc acctgttttg cccgatatgg ctgtccatca ggaccggtca cgctatcagc
    69121 atttgccaga ttactggccg ccacgttcag gcgctgggac tgggcagtta acgccgaccc
    69181 ggcgatatca aaaatattca gcagtgccat ccgttaattt ccgctctgta aaacgttcat
    69241 catgcctttg atttgcccgc tcaacgcgct aaggctcatc tggtattgca ggctgttatc
    69301 ggcaaactgg gtgacttcct gacgctgggc gcgcagattg agcgcctctt gttgaaaacg
    69361 taaggcggcg tcgagcttat cgagcatatc tcctccgcag gtatcaaaat tctgccatca
    69421 gcttaaatgc ctttacgcgc gcgttatcgg cggaataaac gcaaaatggg tcgctattta
    69481 tgccgttgat ggtcattgcg gacaggtaca actcacgttg tagaaatggc tgggggcgaa
    69541 aatgctgaca ataaaacgta gcgtggcgat catcgcgata ctgttcagtc cgttaagtac
    69601 ggcgagcaat ctcacatcgc aattgcacaa cttttttagc gcccaactcg cgggggtaag
    69661 tgatgaggtt cgtgtttcta ttcgtacagc gcccaatcta ctaccgccat gcgagcagcc
    69721 attgctttcg atgagcaata attcccgcct gtggggcaat gtgaatgtgt tggcacgctg
    69781 cggtaacgac aaacgatatt tacaggttaa tgtgcaggcc acaggaaatt atgtggttgc
    69841 cgcgatgcct attgtgcggg gaggaaagct ggaagctggc aatgtcaaac tgaaacgtgg
    69901 acgcctggat accctgccac cgcgtacggt gctggatatc aatcaacttg ttgatgccgt
    69961 tagcctgcgc gatctatcac ccgatcaacc tatccagtta acccagtttc gccaggcatg
    70021 gcgggtaaaa gcgggacaac gcgtcaatgt gatcgccagc ggtgatgggt ttagcgccaa
    70081 cgcagaaggt caggcgctga acaatgcagc cgtcgcacag aatgcgcggg tgcgcatggt
    70141 atcgggacag gtagtcagcg gcgttgttga tgcagatggg aatattctta taaacctgta
    70201 agctgttaaa gattacccgt cccttgccga taaataagca acacatgata aaagcgccct
    70261 caatgaggaa taaaccatga gtattgatcg cacttcgcct ctgaagcctg taagcaccgt
    70321 tcaaccgcgc gaaaccactg acgcgccggt aacgaacagc cgcctccacc agcaccagtg
    70381 tgacgttaag cgacgcgcaa gcaaaactga tgcaacccgg cagcagtgat atcaatcttg
    70441 aacgtgtcga agcgttaaaa ctggcgattc gtaacggtga actaaaaatg gacaccggca
    70501 aaattgccga tgcgctgatc aacgaagcgc agcaagactt gcagagtaac tgatcgtatg
    70561 acacgtcttg cagagatcct cgaccagatg tccgctgtgc ttaacgatct caaaacggta
    70621 atggatcatg agcagcaaca tctctctatg gggcagatca acggcagcca gttgcaatgg
    70681 attacagaac aaaaaagctc actgctggcg acgctggatt acctcgaaca gttacgcaag
    70741 aaagaaccca atacagcaaa tagcgttgat attagtcaac gctggcagga aattactgtg
    70801 aaaacgcagc aactacgcca aatgaatcaa cataacggct ggttactgga aggacagatt
    70861 gagcgcaatc aacaggcgct ggaaatgttg aaaccgcatc aggaaccgac gctatatggg
    70921 gcgaacggtc agacctcaac accccatcgc ggcagtaaaa agatttcgat ctgaatatta
    70981 tctccggcct gcactgcagg ccggaatgca ttgttacacc gtccggcggg caaattcttt
    71041 aactttgaag cccagtaccg ccagtgcagc gaagtacgcg gcaatccccg ccagcacgac
    71101 cgccattaaa cgcagtaagc gccagggcat ggtacccaat gaccactccg gcatgatatg
    71161 taacatacct aaaagcacgc cagacatcac cagtaccgcc accaccagac gcaacagaaa
    71221 cgccatccag ccgggttgcg gagtaaagat tttctgctta cgcaactgcc agtaaagcag
    71281 cgaagcattc agacacgccg ccagaccaat agaaagtgac agcccggcat gtttcaacgg
    71341 accaataaac gccaggttca tcaattgcgt taaaatcagc gtaacgatgg caattttcac
    71401 tggcgtttta atgtcttggc gggaataaaa gccaggagcc aacactttca ctacaatcag
    71461 gccaatcaaa cccaccgagt aggcaattaa cgcccgctgg gtcatcagcg catcaaacgc
    71521 ggtaaattta ccgtactgga acagcgaaac ggttaacgga cctgaaagaa tgcccaacgc
    71581 aaccgcactc ggcagtgcca acaggaaaca aagacgcaac ccccagtcca tcaaacggtt
    71641 gtattcatca tgattgccac tggcaaaact tttcgacagc gacggcagca aaatggtgcc
    71701 aagcgccacg cccagcacac cggagggaaa ctccattaag cggtcggcgt aatacatcca
    71761 agacaccgaa ccggaagcaa gaaacgaggc aaaaatggtg ttgatgatta aggagatctg
    71821 gctcacagag acgccaagga tcgccggtcc catctgtttc accacgcgca ttgctccagc
    71881 atcgtggaag ttaatgcgcg gcaggaccag catgccgatc ttcttcaggt gcggtagctg
    71941 atacaccagc tgcaggatgc cgccgaccgt aacagcccac gccagcgcca gcaccggtgg
    72001 gttaaagtac ggtgcggcaa acagcgcgaa accaatcatg ctgatgttaa gcagtgttgg
    72061 cgcaaacgcc ggaatcgaga agcggttcca cgtattcaga atcgctccca ccagcgacgc
    72121 cagggagatc agcaagatat agggaaaggt aatctttagt aggtggctgg tcagggcaaa
    72181 tttgtcagct gtgtcagcga agcctggcgc ggtcaccatg atcacccacg gtgcggcgag
    72241 catgccagcg accgtcacaa ccgccagcgc aagtgtcagc aggccagaaa cataagagac
    72301 aaagacccgc gtggcgtctt caccctgctt acttttatat tccgccagaa tcggtacaaa
    72361 tgcctgggaa aatgcccctt cggcaaagat acggcgtaac aagttaggaa gtttaaaagc
    72421 gacgaaaaag gcgtcggttg ccatccctgc gccaaagatt ctggcgacaa ttgcgtctcg
    72481 tgcgaagcca agcacacgcg aaaacatggt catcgagctg acggcggcca gcgattttaa
    72541 taaattcatc ggtgttctaa tccggaccca cgggcaaaac gcctgcaagg caggcgctga
    72601 aagaggcgaa tagtctacct agattatggt gaattgctac cgccagatgt tacagggttt
    72661 attcactcat cgcatcgcgc cagatcttgt caacgatacg ttgcgccagc actgcctatt
    72721 cgccggcggt ttgcggaact gtctgatttt gcacgcattc aatgaagtgc cgcgcacagc
    72781 cgacaaatcc acgttgctca agagtgctct gccaaccagg aatcggttta ttcactacac
    72841 cctgcccgcg ctcctcacgc cattcgcgca tatccgtaat gtcgatgagc gcaccgtcag
    72901 tcacggcctg cacggtttca cgctgacttc cggcacggcg atgcatacaa gtggtgatct
    72961 gcaaaggacc ggccgaaaaa tggtgctcgg caaacagcat ttcgccagcg tcgttagtca
    73021 gtagcgtacc gccatccaga gaggctttgc cgcccgacaa ccacagcgcg gtatccacca
    73081 catgcagata atcatccagc aacgtgaaat aaagatcgtg tggcccgacg ctattgctac
    73141 gatgtttatc cattcttagc gaggctgcgg tggcgagttg cgtttttaac tcaccgtaga
    73201 gtggtgcgaa acgacggtta aaaccgacca tcaacgtcag ttttttccgc gccgccagtt
    73261 ccaccagcct ttcagcatcg cgcagatttt ctgccagcgg tttatcgaca cagacatgta
    73321 cccccgcatt gagtaacgta ctgaccacgt caaagtggct ggcggtgctg gagtgcacaa
    73381 aaaccgcatc acaactggcg gcaaggctgg ataacgaatc ggcataagga atgcgccagc
    73441 tgtcacaaat tggcagggct ttggcgcgcg taggcgacca ggctccttgt aacgtccagt
    73501 cagacgctgc cgccagcacc ggtaaccacg ctttttgcgc aatgccgcct aatcccacta
    73561 cgccgatacg taattttttc actttaatct cccagatggg ccagcaacga atcaagacgt
    73621 tgtttcagtt ctgccacttc gatttccagg gcttcgacgc gggtctgtaa atcaccgtca
    73681 accatgttcg acatagccgt taccgccggc tgatcttcaa cctcaccact gaaaagatgc
    73741 atgtagcggc tttcgcgttt acccggttcg cgggccagac gcaccacaaa aggaccatct
    73801 tcgcgattcg ccagttgttc cagcgtcgac tccacttccg ccatatcgct gaattcatac
    73861 attcgcgcgg cgcggctgcg cagttcaccc agcgtctggg caccacgcaa taacaacgtg
    73921 gtgattaacg ccacttctgc ggcgctcagt ttcagatcgc caaattctga attacaaaaa
    73981 cgttgctcat atttggtgac ccgattacca aaaccgctca ctgtgcgtag ataatgacgt
    74041 ttgaccagat tatccagctg ttcctgcact tcggattcac tcagattcat gaccggttca
    74101 cggttcgttt tctgattaca ggccgtgact acaccattga ctgagagcgg gtattgctcc
    74161 ggcgtggtca cctgcttttc cagcaagcaa ccaattacgc gagcttccag ggcagtaagt
    74221 tgatatttca tgagattctc cttagcggcc gggcgtccag tctggggtag ttaatgccgt
    74281 cagtacgtga tcgcgccatt gtccatcaat caacagatag tctttcgcat agccttcttt
    74341 ttcaaaaccc agtcgcgcca gtaaatcacc gctgcgttta ttgtgcggca tataattagc
    74401 cataatgcga tgaatatgtt gggtgcgctg catataacga atggctgcgg tcagggcttc
    74461 aaacatgagt cctttgccct gccatttttg cccaatcgaa taaccgagat agcaggcatg
    74521 aaaagagccg cgaacaacat tggaaaaatt ggcaacgcca ataatctctt tttcgtccgg
    74581 gtcgaataag ccaaagtaga aagctgaacc ttgtttatga aattcgttaa tcatccctag
    74641 cctggcctgc cagcctgatg gataacagtg gctttcgtcg cgcactggct cccagggctt
    74701 gaggaaatgg cgattctctg cgtaataatc cgccagacgc caggcatcac gatcatgcac
    74761 cagacgcacg accagtcggt ctgtggttaa gcgcactttt ggcacgttac tgcgatagcc
    74821 aaacattcta tacctactcc ttcccgtaac gtctttaata cgctgtaagt attactatac
    74881 ctgcgcaacc agaccctgtg aaaagtccaa aatgccattt tctgcattat tcacatattt
    74941 gataaaaact ctctatcaaa gcgacgtttt gataaaaaaa tattgtcccc gaagtgatgg
    75001 gaaaagtctt agcggcaatg cagaatagaa agtgctcatc ttttatttcc cgggagggga
    75061 aatgtcccgc gtatcgcagg cgaggaacct gggtaaatat ttcctgctca tcgataatat
    75121 gctggtcgtg ctggggttct ttgttgtctt cccgctgatc tctatccgct tcgttgatca
    75181 aatgggctgg gccgccgtca tggtcggtat tgctctcggt ctacgccagt ttattcagca
    75241 aggtctgggt attttcggcg gcgcaattgc cgaccgcttt ggtgccaaac cgatgattgt
    75301 taccggtatg ctgatgcgcg ccgccggatt cgccacaatg ggtatcgccc acgaaccgtg
    75361 gctattgtgg ttttcatgcc tgctctcggg actcggcggc acgttgtttg atccgccgcg
    75421 ttcggcgttg gtggtgaaac taatccgtcc acaacaacgt ggtcgttttt tctcgctgtt
    75481 gatgatgcag gacagtgcca gtgcggtcat tggcgcattg ttggggagct ggctgttgca
    75541 atacgacttt cgcctggtct gcgccacagg ggccgttttg tttgtgctat gtgcggcgtt
    75601 caatgcgtgg ttgttaccgg catggaaact ctccaccgta cgcacgcccg ttcgcgaagg
    75661 catgacccgc gtgatgcgtg acaagcgttt tgtcacctat gtcctgacgc tggcgggtta
    75721 ctacatgctg gctgtacaag tgatgctgat gctgccaatt atggtcaacg acgtggctgg
    75781 cgcgccctct gccgttaaat ggatgtatgc cattgaagcg tgtctgtcgt taacgttgct
    75841 ctaccctatc gcccgctgga gtgaaaagca ttttcgtctg gaataccggt tgatggctgg
    75901 gctgttgata atgtcattaa gcatgatgcc ggtgggcatg gtcagcggcc tacaacaact
    75961 tttcaccctg atttgtctgt tttatatcgg gtcgatcatt gccgagcctg cgcgtgaaac
    76021 cttaagtgct tcgctggcgg acgcaagagc tcgcggcagc tatatggggt ttagccgtct
    76081 gggtctggcg attggcggcg ctattggtta tatcggtggt ggctggctgt ttgacctggg
    76141 caaatcgacg caccagccag agcttccgtg gatgatgctg ggaattattg gcatcttcac
    76201 tttccttgcg ctgggttggc agtttagtca gaaacgcgcc gcgcgtcgtt tgctggaacg
    76261 cgacgcctga tttgatacac ccccctgact cctccatact gaaagtgaac tgacagccaa
    76321 atggaggagt cacgtgaagc tatacattta cgatcactgc ccttactgcc tcaaagcccg
    76381 catgattttc ggcctgaaaa atatccccgt cgaattacat gttctgctca acgacgacgc
    76441 agaaacaccc acccggatgg tcggtcaaaa acaggttccc attctgcaaa aagatgacag
    76501 ccgctatatg ccagaaagca tggatatcgt tcactatgtc gataaactcg acggcaaacc
    76561 gttactgacc ggcaaacgtt cccctgccat tgaagagtgg ctgcgcaagg tcaatggcta
    76621 cgccaacaaa ctgctgttgc cgcgttttgc caaatcggca tttgatgagt tttctactcc
    76681 cgccgcgcgc aaatatttcg tcgacaagaa agaggccagc gcgggtaatt ttgccgacct
    76741 gctggcccac tctgacggtc tgattaagaa tatcagcgat gatttgcgtg cgctggacaa
    76801 actgatcgtc aaaccgaacg ccgtgaatgg cgaactttcg gaagatgata ttcagctatt
    76861 tccgctactg cgtaatctga cgctggtagc cggaatcaac tggccaagcc gcgttgctga
    76921 ttaccacgat aatatggcga aacagacaca aatcaatttg ttatcatcaa tggcgattta
    76981 atcctgaccc gccgcggcag cctgtattcg cgggttgccg ccgttttcag ttttttctgc
    77041 tggcgagttt atcgctggtg acacatgctg cgttgtgtca ggatattgac gcagatttca
    77101 tgtcgaggac taccatgaac aagtttttat ttgccgctgc attgatcgtc agtggcctgc
    77161 tcgttggctg taatcaactc acccaataca ccatcaccga acaagaaatt aaccagtcgc
    77221 ttgcgaaaca taataatttc tcaaaagata tcggtttacc cggcgtggct gacgcccata
    77281 ttgttctgac aaacttgacc agccaaattg gtcgcgaaga gccgaataag gttaccctaa
    77341 ccggagacgc caatctggac atgaactccc tgttcggtag tcagaaagcg accatgaaac
    77401 tgaagctgaa agcgctgccc gtgtttgata aagaaaaagg tgcgatcttc ctgaaagaga
    77461 tggaagtggt cgatgcgacg gtacaaccgg aaaaaatgca aacggtgatg caaacgttgc
    77521 ttccctattt gaaccaggca ttacgcaatt actttaacca gcaacctgct tacgtcctgc
    77581 gcgaagatgg cagtcagggc gaagcaatgg cgaaaaaact ggcgaaaggc attgaagtga
    77641 agccaggtga aattgtcatt ccgtttacgg attaatgacg agggcgcatt cgcgcccttt
    77701 atttttcgtg caaaggaaaa catttccgct tatcctttgt gtccggcaaa aacatccctt
    77761 cagccggagc atagagatta atgactgcac catcccaggt attaaagatc cgccgtccag
    77821 acgactggca ccttcacctc cgcgatggcg acatgttaca aactgtcgtg ccgtatacca
    77881 gcgaaattta tggacgggct atcgtaatgc ccaatctggc tccgcccgtg accacagttg
    77941 aggctgccgt ggcgtatcgc caacgtattc ttgacgccgt acctgccggg cacgatttca
    78001 ccccattgat gacctgttat ttaacagatt cgctggatcc taatgagctg gagcgcggat
    78061 ttaatgaagg cgtgttcacc gctgcaaaac tttacccggc aaacgcaacc actaactcca
    78121 gccacggcgt gacgtcaatt gacgcaatca tgccggtact tgagcgcatg gaaaaaatcg
    78181 gtatgccgct actggtgcat ggtgaagtga cacatgcaga tatcgacatt tttgatcgtg
    78241 aagcgcgctt tatagaaagc gtgatggaac ctctgcgcca gcgcctgact gcgctgaaag
    78301 tcgtttttga gcacatcacc accaaagatg ctgccgacta tgtccgtgac ggaaatgaac
    78361 ggctggctgc caccatcact ccgcagcatc tgatgtttaa ccgcaaccat atgctggttg
    78421 gtggcgtgcg tccgcacctg tattgtttac ccatcctcaa acgcaatatt caccaacagg
    78481 cattgcgtga actggtcgcc agcggtttta atcgagtatt cctcggtacg gattctgcgc
    78541 cacatgcacg tcatcgcaaa gagagcagtt gcggctgcgc gggctgcttc aacgccccaa
    78601 ccgcgctggg cagttacgct accgtctttg aagagatgaa tgctctgcag cactttgaag
    78661 cattctgttc tgtaaacggc ccgcagttct atggcttgcc ggtcaacgac acattcatcg
    78721 aactggtacg tgaagagcaa caggttgctg aaagcatcgc actgactgat gacaccctgg
    78781 tgccattcct cgccggggaa acggtacgct ggtccgttaa acaataaaaa tcgtagcttc
    78841 ctgttgtcat taggttattt tacctgtata aataaccagt atattcaaca gggggctatt
    78901 atgcgaattg aagtcaccat agcgaaaact tctccattgc cagctggggc tattgacgcc
    78961 ctggctggcg aactttcccg ccgtattcag tatgcgtttc ctgataatga aggccacgta
    79021 tcggtacgtt atgccgcagc gaataattta tcggttattg gcgcaacaaa agaagataaa
    79081 cagcgcatta gcgaaattct ccaggaaacg tgggaaagcg ccgatgactg gtttgtcagc
    79141 gaataatatg cagtgatttt tgtttttgcc gggtcgcccc ggctttttta ttttacttac
    79201 gaattttgag tacaaaattt ctacacttcc tcaaaataat tgcacaatat gctaaaaaaa
    79261 tatgaatgac atgctgtttt attgttcata taaatcttaa aagcagaaat ttatctgttc
    79321 ccccctttaa aaattcggtg aataccctta cttattggta tactgaagtt gcttcagata
    79381 aaaacgcatt gaacctcgaa taacgttgtc tagtaacacg aattaggggg ccatgatgga
    79441 aaaaaataat gaagtcattc agactcatcc gctcgtaggg tgggacatca gcaccgttga
    79501 tagctatgat gcgctgatgt tgcgtttgca ctaccagacc ccaaataagt ccgagcagga
    79561 agggactgaa gttggtcaga cgctctggtt aaccactgat gttgccagac aatttatttc
    79621 gatattagaa gcaggaatcg ccaaaattga atccggtgat ttcccggtaa acgagtatcg
    79681 gcgtcattaa catgacatga ccatccaccc aaaaggcacc tcaccggtgc ctttaccatt
    79741 tctgggttgt atatctgcat tcagttaatt actctgctta aaccatgaga gcagagagaa
    79801 aaaaatgaaa tacgatctca tcattattgg cagcggttcc gtaggcgctg ccgccgggta
    79861 ttatgcaacc cgcgccggtt taaacgtgct aatgaccgac gcccatatgc caccgcatca
    79921 acacggcagc caccacggcg atacgcgatt aattcgccat gcttatggtg aaggcgaaaa
    79981 gtatgtcccg ttagtcctcc gcgcgcaaac gctgtgggat gaactttccc gccacaacga
    80041 agatgatccc atttttgtac gctctggtgt cattaacctc ggcccggctg actccgcatt
    80101 tctcgccaac gtcgcccaca gcgccgaaca gtggcaactc aacgttgaaa agctcgatgc
    80161 gcaagggatt atggcccgct ggccagaaat acgcgtcccg gacaactaca tcggcttatt
    80221 tgagactgat tccggttttt tgcgcagcga actggcgatt aaaacctgga tccaactggc
    80281 gaaggaagcg ggctgtgcgc aactgttcaa ctgcccggtc accgcaattc gtcatgacga
    80341 tgatggcgta actattgaaa cggttgacgg tgagtatcag gcgaaaaaag cgattgtctg
    80401 cgcgggaaca tgggtaaaag acctgctccc ggagctgcct gtccagcccg tacgtaaagt
    80461 atttgcctgg tatcaggccg atggccgcta tagcgtgaag aataaattcc cggcgtttac
    80521 cggtgaactg cccaatggcg atcaatatta tggttttccg gcagaaaacg acgcgttgaa
    80581 gattggcaaa cataacggag gccaggttat ccattcagcg gatgaacgtg ttccgtttgc
    80641 ggaagtggcc agtgatgggt cagaagcctt cccgttcttg cgcaatgtat tgccgggtat
    80701 cggttgctgc ctgtacggcg ctgcctgcac ctatgataat tcgcctgacg aagattttat
    80761 tatcgatacc ctacccggcc acgataatac actgctcatt accggcctga gtgggcacgg
    80821 ttttaaattt gcgtcagttt taggggaaat agctgccgat tttgcgcaag accaaaaaag
    80881 cgattttgat ttgacgccat tcaggctttc ccgcttccaa taatcatcat tacggcctcc
    80941 agaatgaggc cgtttttaca ggagtattta atgcgtcggc tgttgcacta tctcatcaat
    81001 aatattcgcg agcatctgat gctttatctt tttctctggg gattgttggc aattatggat
    81061 ctgatctatg tgttttattt ttagttttcc gcactgacat aaactgacaa atatttaatc
    81121 tccctttcat ttagcgtgaa aaattgaatg agattattcc tgcttctaca gcactcacca
    81181 gacatctctg atgctgaacc cactaaactt atgaagattc tttcattatt aattcaaaat
    81241 tattgaaata aaaataaata tcattcaatt tgttattttt gtgttgcaat tcattctccg
    81301 acacgccatt ttctttctct cgcaaattcg tattggatgc acttatgtca ttcacaaata
    81361 cccctgaacg ctatggtgtt atatctgcgg ccttccattg gctaagcgca attattgtct
    81421 acggcatgtt tgctttaggt ctgtggatgg tcacgctcag ttattacgat ggctggtatc
    81481 acaaagcacc cgaactgcat aaaagcatcg gtattttgtt aatgatgggg ctggttattc
    81541 gcgttctgtg gcgtgtcata tctccaccgc cgggtccgct gccgagttat tcgcccatga
    81601 ctcgtcttgc cgcaaaagct ggacatctcg cattgtacct attgctgttc gccatcggca
    81661 tcagcggcta tctgatctca actgccgatg gtaaaccgat cagcgttttt ggctggtttg
    81721 acgtccccgc gacccttgcc gacgctggcg cacaggcaga ctttgccggt gccctgcatt
    81781 tttggctcgc gtggagcgtc gtagtactgt ctgttatgca cggatttatg gccctgaagc
    81841 atcatttcat cgataaagac gacactctga agcgcatgct gggaaagtcg tcatctgact
    81901 atggagtata aaaaatgaaa aaaagcctgc ttggtttaac cttcgcgtcc ctgatgttct
    81961 ctgccggttc agcggttgcc gccgattaca aaattgacaa agaaggtcag cacgcctttg
    82021 ttaacttccg catacagcat cttggctata gctggttata tggcaccttt aaagatttcg
    82081 acggtacttt tacctttgat gaaaaaaatc cggctgccga taaagtgaat gtgacaatta
    82141 acaccaccag cgtcgatact aaccacgccg aacgcgataa acatcttcgc agtgcggatt
    82201 ttctcaatac cacaaaatat ccacaggcaa cattcacctc caccagcgtg aagaaagacg
    82261 gtgacgaact ggatattacc ggcgatctga cgctgaatgg cgtaaccaaa cccgtcacgc
    82321 tggaagcgaa attaattggt cagggcgacg acccatgggg cggtaaacgt gcaggctttg
    82381 aggccgaagg caaaattaag ctcaaagact tcaatatcaa gacagattta ggtccagctt
    82441 ctcaggaagt ggatctgatt atttcagtgg aaggcgtaca gcagaagtaa gagtgaaatg
    82501 ttgccggatg gcacgcatcc ggcaatgata tttattctgt tggatcagga atacccagtg
    82561 ttgtattcag acgtccacga gacttattaa agatcttatt gccattttca cgtcctgccc
    82621 gacggcgtcg ctgttcttct ggcggtaacg cgctttcttc gcagcaaatc tcactacaac
    82681 aacctttgta tttttccgcg catactggac actgaataaa cagcaggtgg cagccatcat
    82741 ttttacagtt ggtatggctg tcgcacggcg cgccgcactg gtggcaatgc gcgataatct
    82801 catccgatat acgttcgccc atccgctcgt caaaaacaaa atttttgcca ataaaacgca
    82861 ccggcaagcc ctgctcgcgc gccttacggg cgtattcaat aattccgccc tcgatatgcc
    82921 agactttgtt gaatccgtta tgtttcatcc aggcactggc cttttcacaa cgaatgccgc
    82981 cggtgcagta catgacgatt tttttatctt tatgtgcctg catcatctcg actgcttttg
    83041 gcagctgctc acggaaggta tctgccggaa tttcgagcgc gttttcaaag tgccccactt
    83101 catactcata gtggttacgc atgtcgataa atagtgcatc gggatcgtca agcatggcgt
    83161 tcacttccgc cgcttgcaga tactcaccaa cattgctggc atcaaagtga ggatcgtcaa
    83221 taccgtcggc aacaatgcga tcgcgtacct tcatgcgcag tacccagaag gatttcccgt
    83281 catcatccaa cgcgatattc aggcgtaaac cctctaaagc cgggtcgaag gcatagagct
    83341 gcgcgcgaaa tgtttcaaca ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat
    83401 gcgccagata cactcgccca aaaacattca gcgcggtaaa cagctgatat aaagcgtcac
    83461 gggtcgcctt aggatcggcg atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt
    83521 cgctctcagc caacattttg gcttttaacg cgtcgttgga aatgcggttg tgtaacactg
    83581 gcatggtgta cggttcctgc gagatgggaa agtaaaaatc cgcggcatga tatagcaatt
    83641 atcgataatt aacatccaca cattttacgc tacatttgcg cattaaaaat tatttgttat
    83701 ttacaagcgc ggcaatttcg cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa
    83761 tgcgacaata catacaattg cccgtatagg ttgaaaaaca ggattgatat gacgaatcta
    83821 cccaagttct ccaccgcact gcttcatccg cgttattggt taacctggtt gggtattggc
    83881 gtactttggt tggtcgtgca attgccctac ccgattatct accgcctcgg ttgtggatta
    83941 ggaaaactgg cgttacgttt tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa
    84001 ctgtgcttcc cggaaatgag cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa
    84061 tccgttggca tgggcctgat ggaaaccggt atggcgtggt tctggccgga ccgccgaatc
    84121 gcccgctgga cggaagtgat cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc
    84181 ggcatcctgt tagttggcat ccattttctg acactggagc tgggcgcgcg gcagtttggt
    84241 atgcaggaac cggggattgg cgtttaccgc ccgaacgata atccactgat tgactggcta
    84301 caaacctggg gccgtttgcg ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc
    84361 atgattaaag ccctgaaaaa aggcgaagtg gtctggtacg caccagatca tgattacggc
    84421 ccgcgctcaa gcgttttcgt cccgttattt gccgttgagc aggctgcgac cacgaccgga
    84481 acctggatgc tggcacggat gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag
    84541 ccagatggca aagggtatca attgattatg ctgccgccag agtgttctcc gccactggat
    84601 gatgccgaaa ctaccgccgc gtggatgaac aaagtggtcg aaaaatgcat catgatggca
    84661 ccagagcagt atatgtggtt acaccgtcgc tttaaaacac gcccggaagg cgttccttca
    84721 cgctattaaa tctcccatgc cggatgcttc agaatggcat ccggcattac cacagcaaat
    84781 ccccctgatt tagcgataaa agctctctgg attgcgcccc ctggaagtcg ggcgcataat
    84841 tagtgtgttt atcttttctt cttatgttca ccgcgcctgg cgcaccaaca gcggattgct
    84901 atgtcaccct gtgaaaatga cacccctata aactggaaac gaaacctgat cgtcgcctgg
    84961 ctaggctgtt ttcttaccgg ggccgccttc agtctggtaa tgcccttctt acccctctac
    85021 gttgagcagc ttggtgttac cggccactcc gccctgaata tgtggtccgg tattgtcttc
    85081 agtataatgg gttcctaaac acatcatttt agatattgaa tatgagacgg gcaatcagct
    85141 acatcagatt ttcaagtgaa cgacaactga aaggggactc tgtacgccga caatctaagc
    85201 tggttactga ctggcttgat aagaaccctg agttttactt ggattcatcc ttgagcttta
    85261 aagatttggg gaaaagtgct tttagtggta agcacctgaa aggtggtcta ggtgactttt
    85321 taaccgctat cgagaaaggg cttgttaaag ctggtgatac gctactaatc gagagtttag
    85381 accgtctgtc acgtcaggat attgatattg cttctgagct attacgccgt atcttaaggg
    85441 ctggtgtaga tgtagtcaca ctgagcgatg gagaacatta cacacgagaa agtttaaaag
    85501 acccgttagc cctgattaaa agcattctga taatgcagcg ggcgcatgag gaaagtttga
    85561 ggaagtctga gcgtgtccaa gctgcatgga accgtaaaaa agaactcatc tccgaaggta
    85621 tcaaggtatc taggcgttgt cctgcatggt taaggctgaa tgatgaccgt cgaacattca
    85681 caatcattcc tgataaagtg gaagttgtta aacgagcatt tgacctgaga ttacagggtt
    85741 taagcttttg ggctatcacc agaactttaa acgatgaagg tcacttgtca ttgaatcaat
    85801 acacacccaa acaaaagggg tggtctgata ctgctgtaaa gaaactactc agaaatcgtg
    85861 ctgtgattgg atgtttcaca cctgctggac gtgaagaagt tcagggttac taccccgcaa
    85921 taatcagtga gtcactattc tatcgagtac agcaactgaa cacagggcaa tatggtaggg
    85981 cttctgtatc aagtaatccg ctatcagtca acttgttcag gggtatcatc aaatgttctg
    86041 agtgtggggc tactattgtt cttggtggat atgccttaaa acgttttgga atgtacagat
    86101 gccctaatcg ttcagctaac aggtgtagtg caaaggctat cagtcgtaag caaacggatg
    86161 caactatcct ttacatgctc gcattatgcg accgttttga aactgaaaat actgacacta
    86221 ttgatagcct taaattacag cgtgaagatt tg
//
LOCUS       DAJVQH010000004        82336 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJVQH010000004 DAJVQH010000000
VERSION     DAJVQH010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.004"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQM87_000338"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQM87_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1506607.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQM87_000339"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQM87_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCS1506608.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQM87_000340"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQM87_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCS1506609.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQM87_000341"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQM87_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCS1506610.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQM87_000342"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQM87_000342"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCS1506611.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQM87_000343"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQM87_000343"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCS1506612.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQM87_000344"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQM87_000344"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCS1506613.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OQM87_000345"
     CDS             complement(4598..5320)
                     /locus_tag="OQM87_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1506614.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQM87_000346"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQM87_000346"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1506615.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OQM87_000347"
     CDS             6520..6738
                     /locus_tag="OQM87_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCS1506616.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQM87_000348"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQM87_000348"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1506617.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETEQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OQM87_000349"
     CDS             complement(7472..7672)
                     /locus_tag="OQM87_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCS1506618.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="OQM87_000350"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="OQM87_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCS1506619.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="OQM87_000351"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="OQM87_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCS1506620.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="OQM87_000352"
     CDS             10169..12082
                     /locus_tag="OQM87_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1506621.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="OQM87_000353"
     CDS             12082..13104
                     /locus_tag="OQM87_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1506622.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="OQM87_000354"
     CDS             13098..13316
                     /locus_tag="OQM87_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCS1506623.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /locus_tag="OQM87_000355"
     CDS             13370..14239
                     /locus_tag="OQM87_000355"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCS1506624.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /locus_tag="OQM87_000356"
     CDS             complement(14294..14698)
                     /locus_tag="OQM87_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCS1506625.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="OQM87_000357"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="OQM87_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCS1506626.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /locus_tag="OQM87_000358"
     CDS             15683..17773
                     /locus_tag="OQM87_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1506627.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="OQM87_000359"
     CDS             complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="OQM87_000359"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCS1506628.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="OQM87_000360"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="OQM87_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCS1506629.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19741..20343)
                     /locus_tag="OQM87_000361"
     CDS             complement(19741..20343)
                     /locus_tag="OQM87_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCS1506630.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /locus_tag="OQM87_000362"
     CDS             complement(20333..20500)
                     /locus_tag="OQM87_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCS1506631.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="OQM87_000363"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="OQM87_000363"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCS1506632.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="OQM87_000364"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="OQM87_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCS1506633.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="OQM87_000365"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="OQM87_000365"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCS1506634.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKGDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="OQM87_000366"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="OQM87_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCS1506635.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="OQM87_000367"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="OQM87_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCS1506636.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cobA"
                     /locus_tag="OQM87_000368"
     CDS             26708..28081
                     /gene="cobA"
                     /locus_tag="OQM87_000368"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1506637.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28328..28495
                     /locus_tag="OQM87_000369"
     CDS             28328..28495
                     /locus_tag="OQM87_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCS1506638.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28790..30127
                     /gene="frlA"
                     /locus_tag="OQM87_000370"
                     /pseudo
     CDS             28790..30127
                     /gene="frlA"
                     /locus_tag="OQM87_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30148..31170
                     /gene="frlB"
                     /locus_tag="OQM87_000371"
     CDS             30148..31170
                     /gene="frlB"
                     /locus_tag="OQM87_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCS1506639.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITLLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31220..32050
                     /gene="frlC"
                     /locus_tag="OQM87_000372"
     CDS             31220..32050
                     /gene="frlC"
                     /locus_tag="OQM87_000372"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCS1506640.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIYMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32047..32832
                     /gene="frlD"
                     /locus_tag="OQM87_000373"
     CDS             32047..32832
                     /gene="frlD"
                     /locus_tag="OQM87_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCS1506641.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32932..33663
                     /gene="phoB"
                     /locus_tag="OQM87_000374"
     CDS             32932..33663
                     /gene="phoB"
                     /locus_tag="OQM87_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCS1506642.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33823..34908)
                     /locus_tag="OQM87_000375"
     CDS             complement(33823..34908)
                     /locus_tag="OQM87_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1506643.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34920..36224)
                     /locus_tag="OQM87_000376"
     CDS             complement(34920..36224)
                     /locus_tag="OQM87_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCS1506644.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEMALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36236..36527)
                     /locus_tag="OQM87_000377"
                     /pseudo
     CDS             complement(36236..36527)
                     /locus_tag="OQM87_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 family protein"
     gene            complement(36538..37416)
                     /locus_tag="OQM87_000378"
     CDS             complement(36538..37416)
                     /locus_tag="OQM87_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCS1506645.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37413..38639)
                     /locus_tag="OQM87_000379"
     CDS             complement(37413..38639)
                     /locus_tag="OQM87_000379"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1506646.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38640..39803)
                     /locus_tag="OQM87_000380"
     CDS             complement(38640..39803)
                     /locus_tag="OQM87_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCS1506647.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(39887..40249)
                     /locus_tag="OQM87_000381"
     CDS             complement(39887..40249)
                     /locus_tag="OQM87_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCS1506648.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40266..41171)
                     /locus_tag="OQM87_000382"
     CDS             complement(40266..41171)
                     /locus_tag="OQM87_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506649.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41441..42445)
                     /gene="trpS"
                     /locus_tag="OQM87_000383"
     CDS             complement(41441..42445)
                     /gene="trpS"
                     /locus_tag="OQM87_000383"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCS1506650.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42438..43196)
                     /gene="gph"
                     /locus_tag="OQM87_000384"
     CDS             complement(42438..43196)
                     /gene="gph"
                     /locus_tag="OQM87_000384"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCS1506651.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43189..43866)
                     /gene="rpe"
                     /locus_tag="OQM87_000385"
     CDS             complement(43189..43866)
                     /gene="rpe"
                     /locus_tag="OQM87_000385"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCS1506652.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43884..44720)
                     /gene="dam"
                     /locus_tag="OQM87_000386"
     CDS             complement(43884..44720)
                     /gene="dam"
                     /locus_tag="OQM87_000386"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1506653.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44827..46113)
                     /gene="damX"
                     /locus_tag="OQM87_000387"
     CDS             complement(44827..46113)
                     /gene="damX"
                     /locus_tag="OQM87_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCS1506654.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46205..47293)
                     /gene="aroB"
                     /locus_tag="OQM87_000388"
     CDS             complement(46205..47293)
                     /gene="aroB"
                     /locus_tag="OQM87_000388"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCS1506655.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47350..47871)
                     /gene="aroK"
                     /locus_tag="OQM87_000389"
     CDS             complement(47350..47871)
                     /gene="aroK"
                     /locus_tag="OQM87_000389"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCS1506656.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48272..49510)
                     /gene="hofQ"
                     /locus_tag="OQM87_000390"
     CDS             complement(48272..49510)
                     /gene="hofQ"
                     /locus_tag="OQM87_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCS1506657.1"
                     /translation="MKQWIAALLLMLISGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49422..49826)
                     /gene="hofP"
                     /locus_tag="OQM87_000391"
     CDS             complement(49422..49826)
                     /gene="hofP"
                     /locus_tag="OQM87_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCS1506658.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADIRRTGGKAAKSDADGG"
     gene            complement(49816..50256)
                     /gene="hofO"
                     /locus_tag="OQM87_000392"
     CDS             complement(49816..50256)
                     /gene="hofO"
                     /locus_tag="OQM87_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCS1506659.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50240..50779)
                     /gene="hofN"
                     /locus_tag="OQM87_000393"
     CDS             complement(50240..50779)
                     /gene="hofN"
                     /locus_tag="OQM87_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCS1506660.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50779..51558)
                     /gene="hofM"
                     /locus_tag="OQM87_000394"
     CDS             complement(50779..51558)
                     /gene="hofM"
                     /locus_tag="OQM87_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCS1506661.1"
                     /translation="MTFKIWQIGLHLQQQEVAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51678..54230
                     /gene="mrcA"
                     /locus_tag="OQM87_000395"
     CDS             51678..54230
                     /gene="mrcA"
                     /locus_tag="OQM87_000395"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCS1506662.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54395..54955)
                     /gene="nudE"
                     /locus_tag="OQM87_000396"
     CDS             complement(54395..54955)
                     /gene="nudE"
                     /locus_tag="OQM87_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCS1506663.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55275..57410
                     /gene="igaA"
                     /locus_tag="OQM87_000397"
     CDS             55275..57410
                     /gene="igaA"
                     /locus_tag="OQM87_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCS1506664.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57475..58143
                     /gene="yrfG"
                     /locus_tag="OQM87_000398"
     CDS             57475..58143
                     /gene="yrfG"
                     /locus_tag="OQM87_000398"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCS1506665.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58154..58555
                     /gene="hslR"
                     /locus_tag="OQM87_000399"
     CDS             58154..58555
                     /gene="hslR"
                     /locus_tag="OQM87_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCS1506666.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58580..59458
                     /gene="hslO"
                     /locus_tag="OQM87_000400"
     CDS             58580..59458
                     /gene="hslO"
                     /locus_tag="OQM87_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCS1506667.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59593..61317)
                     /locus_tag="OQM87_000401"
     CDS             complement(59593..61317)
                     /locus_tag="OQM87_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCS1506668.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61696..63318
                     /gene="pckA"
                     /locus_tag="OQM87_000402"
     CDS             61696..63318
                     /gene="pckA"
                     /locus_tag="OQM87_000402"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCS1506669.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63394..64746)
                     /gene="envZ"
                     /locus_tag="OQM87_000403"
     CDS             complement(63394..64746)
                     /gene="envZ"
                     /locus_tag="OQM87_000403"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCS1506670.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64743..65462)
                     /gene="ompR"
                     /locus_tag="OQM87_000404"
     CDS             complement(64743..65462)
                     /gene="ompR"
                     /locus_tag="OQM87_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCS1506671.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65690..66166
                     /gene="greB"
                     /locus_tag="OQM87_000405"
     CDS             65690..66166
                     /gene="greB"
                     /locus_tag="OQM87_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCS1506672.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66263..68584
                     /locus_tag="OQM87_000406"
     CDS             66263..68584
                     /locus_tag="OQM87_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCS1506673.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69022..69249
                     /gene="feoA"
                     /locus_tag="OQM87_000407"
     CDS             69022..69249
                     /gene="feoA"
                     /locus_tag="OQM87_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCS1506674.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69266..71587
                     /gene="feoB"
                     /locus_tag="OQM87_000408"
     CDS             69266..71587
                     /gene="feoB"
                     /locus_tag="OQM87_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCS1506675.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGMGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71587..71823
                     /gene="feoC"
                     /locus_tag="OQM87_000409"
     CDS             71587..71823
                     /gene="feoC"
                     /locus_tag="OQM87_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCS1506676.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72026..72904
                     /gene="rpnA"
                     /locus_tag="OQM87_000410"
     CDS             72026..72904
                     /gene="rpnA"
                     /locus_tag="OQM87_000410"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCS1506677.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            72977..73246
                     /locus_tag="OQM87_000411"
     CDS             72977..73246
                     /locus_tag="OQM87_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCS1506678.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            73240..73605
                     /locus_tag="OQM87_000412"
     CDS             73240..73605
                     /locus_tag="OQM87_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS1506679.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(73606..74394)
                     /gene="bioH"
                     /locus_tag="OQM87_000413"
     CDS             complement(73606..74394)
                     /gene="bioH"
                     /locus_tag="OQM87_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCS1506680.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            74432..75115
                     /gene="gntX"
                     /locus_tag="OQM87_000414"
     CDS             74432..75115
                     /gene="gntX"
                     /locus_tag="OQM87_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCS1506681.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            75174..75749
                     /gene="nfuA"
                     /locus_tag="OQM87_000415"
     CDS             75174..75749
                     /gene="nfuA"
                     /locus_tag="OQM87_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCS1506682.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            76110..77414
                     /gene="gntT"
                     /locus_tag="OQM87_000416"
     CDS             76110..77414
                     /gene="gntT"
                     /locus_tag="OQM87_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1506683.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77459..79543)
                     /gene="malQ"
                     /locus_tag="OQM87_000417"
     CDS             complement(77459..79543)
                     /gene="malQ"
                     /locus_tag="OQM87_000417"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCS1506684.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTTTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79553..81946)
                     /gene="malP"
                     /locus_tag="OQM87_000418"
     CDS             complement(79553..81946)
                     /gene="malP"
                     /locus_tag="OQM87_000418"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCS1506685.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        19753 a        22361 c        20767 g        19455 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaacagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctgctcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcactcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg
     9001 ctgggatcac tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccacgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccaac cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctttg cgctttgccg tctcgcccag taaataatga tggaacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg
     9661 gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgtt
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgcca gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat agcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc agatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtggcac atctgcaaag ttatatcgat cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctggaa cgtatggagc tgattgcccc
    11041 cgcacacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaattgaat ctggtccccg gctcgcgcat tggtctgtta ggccgcaacg gcgcaggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcaaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttcctggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaggccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cagtcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccaaa accggaaagt
    12961 ctcccctcgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggaact gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aatagtgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccctgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtagt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcggtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccttg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtacctgg
    14161 cggcaaaatg gggctggcaa tggaattaat tatgctgctg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tttgctatta tgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aagtcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctccctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacactt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggtaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gctggggcca ttgcacgcaa aattgcttcc
    16021 tgcatcgctg ctcgccgcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accattgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgatcctg aaaaagcgct
    16261 gccaccgctg ctggtgcgcc agcaaaaagc ggtcgattta attactcagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaacaatac cgattacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttgcagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaaactggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttgc ttccggcact
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgt gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtagcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg caacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccacgt cttatcgata ccattattgg
    17161 ttgtttgatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgatg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggacccg caaccaacgc cactggcctg gcagagaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcattcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggccatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatacaggc taaaccatcg gataaggcat tcacgccgca tccgacatat
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccctc atcgatatcc gcatcttcca ccaccagcga cggtgctaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccaa cctctgcacc cgcatacagg aaatcacgcg
    18001 cccgaccttt gtagtgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacaccgt agtacctgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgcact tcatcaaaca ccaataatac ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgca tggagatcgt
    18541 taaagggaac gtggatgatg tctgccggtt gcggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataaa tatgaaatta taatcacaaa atatgcataa aaaatcactg aatggcaatc
    19141 ggaaatcaga gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ctatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttgccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcactatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gtgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaagcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtc gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tggtacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac atcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtaatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcgacg gcgatgttca ttctcggggt ggtcagcggc
    21781 attactatgt cgattggtac attcctgata acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttactga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc agctgtgagt tcccggcact gggcaaacgt
    22021 gcaccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23221 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtaaa
    24661 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24721 ggcgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24841 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25561 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    26041 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26161 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtagtcgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27061 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27181 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27241 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27301 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27541 gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27721 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27961 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28081 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28141 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28201 cttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa
    28261 cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg
    28321 aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac
    28381 tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca
    28441 cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc
    28501 tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg
    28561 tatttgtttc tatgtccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat
    28621 tttataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc
    28681 acattacaca acatcatatg ttgttatatt catcatgcat tgtcatgttg ccttttaaat
    28741 gactgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca
    28801 ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt
    28861 cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct
    28921 tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc
    28981 ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg
    29041 aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc
    29101 attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc
    29161 gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg
    29221 ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt
    29281 cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccttac
    29341 caccactgcg attggcgcaa cgggcagctt tatggcgctg ctggcgggga tctctgccac
    29401 cagttggtcg tataccggca tggcctctat ctgttatatg accggcgaaa ttaaaaaccc
    29461 cggaaaaacc atgccacgag cgctgattgg ttcctgtctg ctggttctgg tgctctacac
    29521 cctgctggcg ctggtgattt ccggcctgat gcccttcgac aaactcgcca attctgaaac
    29581 gccgatttcc gacgccctga cctagatccc cgcactcggc agcaccgctg ggatctttgt
    29641 tgccatcacg gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga tgtaccagcc
    29701 gcggctggaa tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg gccatgtgca
    29761 tccgaaatac aacacgccgg atgtctccat catcctgcaa ggggcgctgg ggatcttctt
    29821 catcttcgtt tccgatctca ccagcctgct gggttatttc accctggtga tgtgtttcaa
    29881 aaataccctc accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt acaaaccgct
    29941 gtggcgtact ccggctttcg ggctgatgac caccctcgcc attgcgtcaa gcctcattct
    30001 ggtcgcctca acctttgtct gggcaccgat tcccggcctt atctgcgccg tcatcgttat
    30061 tgctactggt ctgcctgctt acgccttctg ggcgaagcgt agccgccagc tcaacgcttt
    30121 gtcgtaatgt tatctgaaga aaataaaatg ttggatattg ataaaagcac cgtggacttt
    30181 ctggtcaccg aaaatatggt tcaggaagtg gaaaaagttc tcagccatga cgttccgctg
    30241 gtgcacgcca tcgtggaaga gatggtgaag cgcgacattg atcgtattta tttcgttgcc
    30301 tgcggctcgc cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg cttttccgat
    30361 cttcaggtct acgccatttc cggctgggag ttctgcgata acaccccgta tcgcctcgac
    30421 gatcgttgcg cggtgattgg cgtttctgac tacggtaaaa cggaagaggt aatcaaagcg
    30481 ctggagctgg gccgggcctg cggcgcactc actgcggcgt tcaccaaacg cgcggacagc
    30541 ccgattacct cggcggcgga atttagcatt gattatcagg ccgactgtat ctgggaaatt
    30601 cacctgctgc tctgctacag cgtggtgctg gagatgatca ccctcctcgc gccgaacgcg
    30661 gaaatcggca agatcaaaaa cgatctcaag cagttgccga atgcgctcgg tcatctggta
    30721 cgcacctggg aagaaaaagg ccgccagctt ggtgaactgg ccagccagtg gccgatgatt
    30781 tataccgttg ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat tgtaacgctg
    30841 atggaattta cctggacgca cggctgcgtg attgagagcg gagagttccg ccatggcccg
    30901 ctggagattg tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga tgaaagtcgc
    30961 cacaccaccg aacgcgccat taactttgtt aaacagcgta ctgacaacgt gatcgtcatc
    31021 gattacgccg aaatttcgca agggctgcac ccgtggctgg caccgttcct gatgttcgtg
    31081 ccaatggagt ggctctgcta ctacctgtct atttacaaag atcacaaccc ggatgaacgc
    31141 cgctattacg gtggtctggt ggaatattaa tccctctccc ggcccggcaa cgggccggat
    31201 ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc agcggctgcc
    31261 tattgaacac gcatttcgtg atgcaagcga gctgggttac gacggcatcg aaatttgggg
    31321 cggtcgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac aaatcaaggc
    31381 gctggcgcag acgtatcaga tgccgattat cggttacacg ccagaaacca acggctatcc
    31441 gtataacatg atgctgggcg atgaacatat gcgtcgtgaa agcctggaca tgatcaagct
    31501 ggcgatatat atggcaaaag agatgaacgc gggttatacg ctgatttctg cggcccacgc
    31561 gggctatctc acgccgccta acgttatctg ggggcggctg gcagagaact taagcgaact
    31621 gtgtgagtac gcggaaaaca tcggcatgga tctgatcctc gaaccgttaa cgccgtatga
    31681 atcgaacgtc gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg tgccttcgcc
    31741 gcgcctgttc agcatggtcg acatttgcgc gccgtatgtc caggcggaac cggtgatgag
    31801 ttatttcgac aaactgggcg ataaattacg tcatctgcat attgtcgaca gcgacggggc
    31861 cagcgacacg cattacattc ctggagaagg caaaatgccg ctgcgggaac tgatgcgcga
    31921 tattattgat cggggctatg agggttactg tacggtggag ctggtgacga tgtatatgaa
    31981 cgagcccaga ctctatgccc gccaggcgct ggaacgcttt cgcgcgctgc tgccggagga
    32041 tgagagatga aaaccctagc gacaatcggc gataactgcg tcgatatcta cccgcaactg
    32101 aataaagcgt tttctggcgg taatgcggtc aatgtggcgg tgtactgcac tcgctacggc
    32161 atacagccgg gatgcattac ctgggtgggt gacgatgact acggcacaaa gctgaagcag
    32221 gatctcgccc gcatgggcgt cgatatcagc catgtccata cgaaacacgg cgttaccgca
    32281 caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac cgaaggcgtg
    32341 atggccgact ttgccctgag tgaagaggat tacgcctggc tggcgcagta tgacattgtg
    32401 cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc tgcgggcaaa
    32461 cttaccgctt tcgacttctc cgacaagtgg gacagcccgc tctggcagac actggtgccg
    32521 catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg tctgaagatg
    32581 aaagcgattg ttgcccgtgg cgcaggaaca gtgattgtca cgctgggtga aaacggcagc
    32641 attgcctggg atggcgcgca gttctggcgt caggctcctg aaccggtgac ggttatcgac
    32701 accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc tgcggggatg
    32761 acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cgaaaaccat tcagtaccac
    32821 ggtgcctggt aggtataacg ttggcgtgag catcttcacg ccaacgtgct gttacttgcc
    32881 ggaaaacgac cctataatcc gagtaattca ttctttattt cagggtcgat tatgtcagct
    32941 acggaccgct actctcatca actcctctac gctaccgtcc gccagcggct gctggatgat
    33001 atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaacga gctttgtaca
    33061 caatataacg tcagccgcat taccattcgc aaagccatca gcgacttagt ggcagacggc
    33121 gtactgatcc gctggcaggg aaaaggcacc tttgtacaaa gccagaaagt tgaaaacgcc
    33181 ctgcttactg tcagtggttt taccgatttt ggcgtctcac aaggcaaggc gacgaaagag
    33241 aaagtgatcg aacaggaacg ggtcagcgcc gcgccgtttt gcgaaaagct gaacatcccc
    33301 ggaaacagcg aagtgttcca tctctgccgg gtgatgtatc tcgataaaga gccgctgttt
    33361 attgatagtt catggatccc gctgtcgcgt tatcctgact ttgatgagat ttacgtcgaa
    33421 ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt cagcgacaaa
    33481 aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa atgcgaactg
    33541 ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa accggtgcac
    33601 gtctccgaac tcttctgccg cgccaatcgc atcaccttaa cgattgataa taaaagacat
    33661 taaccgtagg ccggataaga tgcgccagca tcgcatccgg cgatgctggc gcactgaatt
    33721 ttacatcccg cacgttcccc tcaccctaac cctctcccca aaggggcgag gggagcgttc
    33781 agtgcggaat ttagcttgat tgccgaacgg tgctatccag cattaaatac tcgcaatact
    33841 ctcacgcaaa atccgcagca ccgtctcttc accgctgcga ttcgggttaa tgcgaatcgc
    33901 acaatgttcg agttgtggat tcgcctggcg aaacgttcct gaaagtcgat agaagagcgg
    33961 cgggatttca tatttcgact ctgcaccgac cgggtaaggc aacgcgccac gcttttgcgc
    34021 ttcttccagc accctggcgg caatcagctg atgaaactcg acaatcaaca ccttcgactg
    34081 cgcattggca atgacggcgc ttttcacttc cgccaccgcg ccgccgttaa gcaaagccag
    34141 caaccgttcg gatactcccg cctgcaccgc atgcatcact ggcgcgaaca ccagaccacg
    34201 caagacttcc agcgcctgtg agccctggat ctggctaccg ccggagtaaa gcgtggcgcg
    34261 aatccgacta ataacatcgg catcgccaac cactgcacca acgccctccg gcccaaacag
    34321 cttgaagcag gagaatgtcg agacattcgc gccgcattca cagccgattc gcgctacctt
    34381 catcaccgca tagttatcat cggttaacgc tggaacacct gccgcgcgca acgttgccag
    34441 cacatctgcc agcacatagc tgtcctgcgg ctgctggcgc gtatgctgca ccagcgcggc
    34501 atcaggttgt tgctcgtcga cgacctgctt cagtgccaac aggtcattga aatcagcagt
    34561 aataagcgtc agccccatct gctcaataat aacccgtgtc gtcgggtaaa caggcgcgtc
    34621 atgcaccaga agacgctgcc ccggtttgag caaagccgcc agcccggcgc gaatcgcgcc
    34681 agtccccgcg ccctgcacca gcgccgcggc ctgtgcgtga aatgcatcag ccagcacctg
    34741 ctccacccgt tgggtaatac gcggttgatt cagacctgga gttaacccta aatcaccgcc
    34801 agcaagaaac tcgctgcctg gaaaatggcg gcaaatagtg tccaccagcg caaactgctt
    34861 ttgctgcgct tcagcaagag tcaggctttg caaaggaaac gtcttcatag gagccttcct
    34921 taagccggaa cgaacaggcc aagccagtaa agaacattca gcagaatacc ggtgatcatt
    34981 actgccacga ccggtgccgc cattttctgt accggacggc ccagggattc gttgaggaag
    35041 taaatagcaa ccgcgataga gaatccggta taacccgcca tcttaattgc tgcgaaaatc
    35101 gaaccgacca gcagcgccat ttccatcagc atattcatgg cgttacggat gttatccgac
    35161 gcattacgca ccgacgggta gcgtcccagc catttgccga tcgaacgaag cagcaagact
    35221 tccgccgaaa taaccactgc gcctaatacc gctgcaacca tcggattcgg cgagagatag
    35281 cccaccgcat aaacaaaggt aaagcccgca actgcataca cacccgttgc taaggcggtg
    35341 gtggcaatca acggcacaaa ccccagtccg cgcataaatt ctgccagagc cgcctgatta
    35401 atcagcgttt gcgattgttc cggcgtcacg cctgcggaat acgctttctc cagtgtgaag
    35461 atcgaaactt cgctaccagc aaaaatcttc atactggcaa cggcggcaat caatgctccc
    35521 acgatggcga tataaggtaa gtttttgata atccgcgacg tgcgctcttc aaacaccgaa
    35581 agcccgctgg catcgatgtc attttcatca cgatgacgca ggtcgtgagt tatcgcgatc
    35641 cccagcagca tcaccatgcc aataaagatt tcgatggatt cagggttaag atgtgggaaa
    35701 tagcgcacga ctaccacacg ggtcatcaga acaaccacgg cggcgaccag actttgcttc
    35761 cagccaaact ggtagaaaat cgccaccagc gggaacagtg caaaagcaga aaccaccggc
    35821 gagcttaatt ccccgaggct acctaataca tccaccggca gcgcggtcag cagctggtta
    35881 actggcaaca ggcaagtaag gatcaacacg ccccagatag cgccaagacc aaacgccatc
    35941 aggctgttta tcgccagtac gccgaggata tcggtaggaa gaaagagtaa ccatgcgttg
    36001 agcagccagg ttttcagggt aaaagagatc cccaccgagg ccacaaaacc aatgctcaga
    36061 ccaaaagcga tactccccgc ctcgcgacgg ttcatatagc cttcaatcag ttgcggcagg
    36121 atcgggtgga tgccgtcatg aaaaacagcc gccgagcgat gcgccagaag cgatgtcata
    36181 cccgtcaggc acgccaccac gataatctga atatacagat ccatagtcgt gccttttatt
    36241 tcagatggtt aatcaacatc ggaatcgcgt gttcgacgtg ctcaacggaa aggccaaacg
    36301 ccactttccc ttcagcgatc attttggcga tatgctcgtc tttcgcttta tgccgcgccc
    36361 gcaccggtat tacacgcgcc aatgtaataa tctagttgcc cggatttcac cttcattgcc
    36421 gcttccatgt cgttgtgaat gaaaacttca aaacagccag gagccgtcgc ttcaatggtt
    36481 tttttaatct gctcacgctg taagcctgca acgccaatct ttttcatgag tctgtcctta
    36541 ttggaaaaat tgagagggat tttcacgtaa catcacatcc acatcggcct gactgaatcc
    36601 tgactggcac aattgcggaa taaaggtggt caataaatag tcatagccat aaccaccatt
    36661 ggcttttaaa tgggagcggc gcgtaatatc catcgacagc atgacgcggt tcagcaaccc
    36721 acggtcacgt agcgcgtgga gcatcgcaat gcgcttttcg tccgggtagt aactgttctt
    36781 accaatggtg tcgaactgca cgtacgcgcc gagatcgatc atcttcaaaa tgttgtcgag
    36841 gttgtctttc agatcgcagt gaccaacggt gacgcgcgaa agatcaactc cgtgggcttg
    36901 caacagagcc agttgttcca gccccatggt gctgaacgac gtatgcgtgg agatcgggcg
    36961 tccggtctgg ttatgcgcca gcgcagcggc aataaatacc ttctcttcca gcggcgtaat
    37021 ctttccttcg ctggagccga tctccgcgat gatcccggct ttcagctccg tgccatcgat
    37081 accctgttca atttcatcga ccatctcctg cgccagttcc tgcacgctgc gggtcaccac
    37141 atgttccggg aaaaacgcat cctggtaata accagtacag gccaccacgt tgatcccggt
    37201 ttcgcgcatt acatcgagca taaattgcgc attgcgcccc atgtaacggt tggtcatctc
    37261 aatcacatta cgtacacccc gggtcatcag gtcgttcatc tcctggcaaa tgaacgcata
    37321 ctgatcaagg cggcagtcca cgttgttttt aaagccggag agatcaatat gcagatgctc
    37381 atgggccagg gtgtaacccg tcggatcaaa actcataggg tgtctcctgc aaaccggagg
    37441 gaggaaagaa aagagcgacc attttgtggc ggtggcgcac ggaaaaattc acacacggta
    37501 gctcccacat cagaaagcgt ggtgcgcaca ccgagctgcg tggcgaccag cccttgctga
    37561 taaaccagca ctggcaccac ttcacgggta tggtggctgt gaccaatggt cggatcgtta
    37621 ccgtgatccg ccatcacgac caggcaatca tctggctgca tcgcctcaac aagccgggca
    37681 aggttacggt caacgacctg caaacgttcg gcataacgtg cgacgtcttc tgcatgacca
    37741 gcgaggtcgg tttcctggat attgatgcaa ataaacgccg tcggatgggt gttaaattcg
    37801 tcgagggtga tatccataat ccgctggcta tccaccaggt tttgccagct cacaccataa
    37861 ggattgctga cgatatctgc caccttaccc accagcacgg ttggcacgcc cgcttcatac
    37921 agtttttgcg gcacctgcac tttttcatca acaccgtagc ccatatgcac gacctggaag
    37981 ccgttgtcat aagcgccaga acgcggcgca ttaataccaa taaagcgccc ttctttgctt
    38041 tctgcggcat cgagaatacg ctgactgttg gttaacaggc cgccaaatgt aatgacccga
    38101 ccgacctgta cctggtcacg cacgatacga ccaattttga ttgcgtcgtc aaaagagatc
    38161 acagagagat tggcggtaat gttatagact tggcctaaat ccgcctcgag attatcgcca
    38221 atcgcaaccg cctgattgac ccacagaaat tgcagatcat cgccacggcg ctccacctgc
    38281 cagccagcgg aaactaatgc ctgctcaaca cggtcaatca catcgcgaaa aggcatccgc
    38341 agcggcggta acgggcgcgt gcctaaaatt tcctgatgcc ccataaaggt atcgccacct
    38401 tcatgttgca gctctgccac gccccaggtt gcggaatctg acggctgcat atcgcctggc
    38461 gcataaccca atgtgttgat tagccccagc ttctccaacg ttggtagctg caaatgcggc
    38521 aactggctca ggatgtgacc acaggtattc gccccggcat cttgcggacg caccagcgtg
    38581 acatctttca ttgcccctac gccaaagcta tcaatcacta acaccacaaa tcgcgccatt
    38641 tatgccttca gagggttgcc gaggctgtca taacgaccaa ctatttctgg ttcgccgcgc
    38701 tgaataccgg acaccagcac cacatcgcta cgggtgacaa aaatctgcgt gcgaaaacag
    38761 agcaccactg cactgctcac cggatactcg cctgccagcg gcagggtata gtcgatactg
    38821 ctgtcatcca cggttttgag attggtttca gtaatctttt gattttctgg cgtaaacacc
    38881 agcgcatgtt gcgcatgacc acgacggtag taaccgccgc cgtagcagta gctgtcgcca
    38941 cggaaatgat gggagatttc acttaaccag agcatcgcga tacgttcagg ctgatcgccc
    39001 tgctggtttg ccggaatagt accggtcagc gcatgaccgg gttcggcatg agtcgcaccg
    39061 tattccgcca gcaatggcag cgaagtgcag ctggtcgctg aaggtacgtt cagttgctca
    39121 atcgcaatac cagatttcgc cagttgatcc cgtgcttgta ccagcgtgtg aagattcggt
    39181 gttggcaaaa ctttcccggc agcctcatcc caaagcaggc aagggaaatg ggttagtccg
    39241 gctaaatgca gccccggcag tttcttaatt tcagcaatca cctcgtgcag cgaatgctgt
    39301 acaaaaccgc tctcctgccc cggataaaga aaatcatcgt cgctataaac tttgagcagc
    39361 acaggctgga cccgtccggc cttaaccgct gccgcagaaa cttcccgtgc tttgtcgaga
    39421 gtaaacacgg tgatgatgtc ggtgccctgt tcaacggcgt cagcaacctg atgacaaggg
    39481 atttgtacca gatgcccctg atgcgccact ggcaaaccag cgcggcgcat gactcgcgcc
    39541 tctttgtaat ccaccgccac aatgccgctg tagcctaatg ccagcaattt ttccgccagc
    39601 cacggattac gaccaaattg cttggtcatc agatacagtt caatgccgta aagccgcgcc
    39661 gtctcaatga gccgcttacc gttttccagt atctgatcca cgtcgatcac ccagctgtcc
    39721 ggggcgattt tgccctgctg ccacaaactt agtgcggcgg aaatcagcgc cgggttctgg
    39781 cgtttcaatg cttctacaaa catcttgttg cctcgtttct gttccttgaa ttaaatattc
    39841 atttttttaa atatttaggt tgtgcgacgt ggatttgtcg ggagcttcaa acctcttcgt
    39901 tagcagccat ccataatcca taaagattgg ccagaaaata gccttcttca cacgggttaa
    39961 cttccagagc gaattccttc aacaacacct gatgcaattg cacaacggct tgccagtggc
    40021 tggattgcgc cagttccgcc agcaactcgt tatccagcgg ctctatttct tcaccacggc
    40081 gactgcgcat cagtgcactc gccatatgtg tcatcgccat cgttccttgc tcactgcgca
    40141 ccggcagatg gcactctttt tccagtacgt tgacgacctg catcatgcct ttgcagacgt
    40201 ccttatcaat aacgcctgcc tcgcaaagca ggttgagtct ggtttccata tcagatgcct
    40261 ttcgtttaat aactgggttc ctgttcccca ctcaccaccc gttgttgatg ggcaagcagg
    40321 gcgtgtttat ctacaacggc acgcagcaat tgttgcattg ggtaatcatc gactcgcgtc
    40381 aggaccacgg cttcggtggc cagtaaaaat cgcggatcgt ctgttagcgg cgtcgcctct
    40441 aatcccagca tggtcagttc gttttccgcc accacgttcc agatcaccgc atcgacatcg
    40501 cctttaacaa tgcgttgtaa actctcgtga taagagagat cgactcgttc cacatcacta
    40561 tcgccaaaaa agacatcggt catgattttc tgatccgccg aacgactatc gagacctact
    40621 cgcttaacat ttccagactc acctttgcgg catatcagtt gatgttcacc aacatacgta
    40681 tgcgggccaa gctcgagggc gatgcacaaa ttgttttggc tgagataact ttccgccgcc
    40741 aggcgggaaa ctactgccat gtcatagaca ccgttaagta aacactccac gcgaatatcc
    40801 gcgccgcgca tatgcgcata gtaaaaagga atgccatcaa actgggcttt caatccgctc
    40861 gctaaacctt cgtacaagcg ggtatagggc aaaggcatcg cacataccac gttgttgata
    40921 tccacatgac tcagcaatgc tttgttatcc atctcgacaa gataactgcc attgcgccca
    40981 cggcgttcaa tccgtatcgc tccgcttgat tccagcgttt tcaacgcggc ctgcgtcagg
    41041 ccaaccgatg aacggcattc agttgccagc tcatctatgg ttttcagtcg attaccgcat
    41101 ttttcaccca acaaataacg ggccagtgtc gtgatgacga cgccttcttt tttgataaac
    41161 gttcgacgca taatatattt tcaataaaat gaatatttat atcttcatta aaatgaagat
    41221 aagtggcgac gacgagatgt ggaaacggcg aggcgcttca cattttttcg ggttgttgcg
    41281 aaaagaggat aaaaaaaccg ggtttccccg gtttcagagt gatgacgaag tgatttattc
    41341 gacaatgcga gcaaagggta attcccactg gcgcttgcct gctcccctca ccctaaccct
    41401 ctccccaaag gggcgagggg accgtccgag cgttttgata ttacggcttc gccacaaaac
    41461 caatcgcttc gtacaccgct ttcagcgtac gggaagcgtg tacactggct ttttctgcgc
    41521 cgtctttcat cacctgttgc aggaaggctt catcgttgcg gaaacggtga tagcgttcct
    41581 gcaattcagt cagcataccg gaaacggcat cagccacttc acctttcaga tgaccataca
    41641 tcttgccttc gaactgtttt tccagttctg ggatgctctg gcccgttacc gcagaaagga
    41701 tatccagcag gttggaaacg cccgctttgt tctgcacatc gtagcgaact accggcggct
    41761 cgtcggagtc agtgaccgcg cgtttgattt tcttcactac cgatttcgga tcttccagca
    41821 ggccgataac gttattgcgg ttatcgtcag acttggacat cttcttggtc ggctccagca
    41881 gcgacattac gcgcgcgcca gatttcggaa taaacggctc cggcacctta aagatctcgc
    41941 catacagcgc gttgaaacgc tgggcgatat cgcgactcag ttccaggtgc tgtttctggt
    42001 cttcacccac cggtaccaga ttagtttgat acagcaggat gtccgcagcc atcagcaccg
    42061 gatagtcaaa cagaccagcg ttgatgttct cggcataacg cgcagattta tctttaaact
    42121 gcgtcatgcg actcagttcg ccgaagtagg tatagcagtt cagtgcccag cctaactgcg
    42181 catgttctgg tacgtgggac tgaacaaaaa tggtgctttt ctcaggatcg ataccacaag
    42241 ccagatacaa ggccagcgta tccaacgtcg ctttacgcag cttctgtgca tcctggcgca
    42301 cggtgatcgc gtgttggtca acgatacagt aaatgcaatg gtagtcatcc tgcatgttta
    42361 cccactgacg cagcgcaccc atgtagttac caatggtcaa ttcacctgag ggctgtgcgc
    42421 cactaaaaac gatgggctta gtcatttttc gattcctgat tttcgctatg cggaagccct
    42481 aatgcgggca gaaggtcatt tatagactga taaattacat caggctggct gagatcgata
    42541 gcctcgccgt agttatatcc gtaggttaag ccaactgatg ggcaacctgc cgcttttgcc
    42601 gcctgaatat cattgcgtga gtcgccgaca aacagcatct gttgtggggc aattcccatc
    42661 cgctcagcca ccagtaacag cgggtccgga tgcggttttt tgttttgcac atcatcgccg
    42721 ccgataacca cgctaaagta tttggcaata tctaaggctt cgagcagcgg cgcgacgaac
    42781 ggcgtcggtt tgttggtgac caggcctagc ggcaggcctt tagcctgcaa cgcgcccaac
    42841 gtatcggcaa cgtgcgggaa caaaaacgtc ccctcttcgg caacctcgcc atagtagcga
    42901 tcgaacagtt tacgcagaat acgtacctgt tcttctgccg gaatgtcgtc atcaacgggc
    42961 ggtttcccca ttgttttacg cagagtcgca cgttcctgac gcgcccaggt caatgcgcgc
    43021 tccatcagaa catctgcgcc gttaccaatc caggtaataa cgcgttcttc acctgcgacg
    43081 ggcaactcca gcgcatacag cgccatatct accgcagcag caagaccagg agcactgtcg
    43141 accagcgtac catcaagatc aaaagcgacg ccgcgaatat cttcaaactt attcatgact
    43201 tacctttgcc agttcactgc gcatttcatc aatgactttt ttatagtctg gctggtcgaa
    43261 gattgccgaa ccggcgacga acatatccgc gcccgccgca gcgatttcgc caatgttgtt
    43321 caccttcacg ccaccgtcca cttccagacg aatgtcaaag ccagactcgt cgatacggcg
    43381 acgtacttcg cgcagtttat ccagtgtttg aggaatgaaa gactgaccgc cgaaaccagg
    43441 gttgacggac atcagcagga tcacatccag cttatccatc acgtaatcca gatagctcag
    43501 aggtgtcgcc gggttaaata ccagacccgc tttacagcca ttttctttaa tcagttgcag
    43561 cgtgcggtca acatgctcgg aggcttctgg atgaaaggta atgatgctgg caccagcggc
    43621 agcgaaatca ggcacaatgc gatcgacggg tttcaccatc agatgtacgt cgataggggc
    43681 ggtaatgccg tagttacgca aggatttcag caccattggc ccaatcgtca gattgggaac
    43741 atagtggtta tccatgacgt caaaatgcac gacatcagcg ccagctgcca gggcttttgc
    43801 ggtatcttca cccaggcggg caaaatcagc cgacagaatt gagggggcaa tcaaatactg
    43861 tttcatccgc ttctccttga gaattatttt ttcgcgggtg aaacgactcc tggtttgtac
    43921 aaagccagca gttcgtccac ctttttacgt gtgccgccgt tgctgcttat actgcgtcga
    43981 actttgacga catgcaattt tgcgcgctga taccactcac gcgttaacat cgtatcgtga
    44041 ttggagatca gcactggaat atggcgctca accagacctt cggcgatctc cgccagatgc
    44101 gcttgttgtt caagcgtaaa actgtttgtg tgatacgccg taaagttggc ggtcgcagac
    44161 agcggtgcat aaggcggatc gcaatagacg acggatgcat catctgcgcg ctccatgcta
    44221 tcggcgtaag actcacaata gaaaaaggca ttctgcgctt tttcagcgaa gtgatacaac
    44281 tctgcttccg ggaaataggg ttttttgtag cggccaaacg gcacgttaaa ctcaccgcgc
    44341 agattgtaac gacacaggcc gttgtaaccg tagcggttca aatataaaaa cagtaccgcc
    44401 cgacggaacg gatcctggct tttgttgaac tcttcgcgga actgatagta aacctcggcg
    44461 caatttgttt cgggaacaaa cagctcgcgt gcggcctgta cgtactcatc agtacgcatc
    44521 ttcacaatgt tatagagact gatcaggtcg ctattgatat cggcaaggat ataacgagaa
    44581 aagtcggtgt tgagaaacac cgacccggca cctacaaaag gctcaaccag acattcgccc
    44641 ttgggcaaat gccgtttaat atcatcaagc aggggatact tgccccctgc ccacttcaaa
    44701 aaagcgcgat ttttcttcat gccgactaac taattacacc ttctccggct gtggagaaag
    44761 ctccgacagc atccagcgct tcaggcagct cccgcaacga taaaccattg cgggagataa
    44821 ccttgattac ttcagatcgg cctgtacctg acgcagcggt ttcgcccacg ggtttttggc
    44881 ctgaacatct gctggcaatg tagataccgc ttttttcgcc tcttctttcg aagcgtacac
    44941 gccagaaacc aggacatacc acggctgacc attacgcgtc gtttcataga caacgtagtt
    45001 tttcagattc tctttcttcg cccaaccgtt caggttgtcg tagttagagg aactgctcag
    45061 ctgcagagtg taatggctgg acggtgccga tttcaacgaa ccaacattac ctgcggtctt
    45121 ccctccagcg gctggtgttg ccgtggtttg cgccggggat gccgtctgta ctggagccgt
    45181 agtcgccgtc tcttttggtg ctggcgtaga agtcgcagcc ggtgccttcg tgctcgccac
    45241 aggggcagct ggttcagtac gcttcggcgt ctgtgctacc ggcttcggct ccgttttcac
    45301 ggttgcttgc ggttttttcg gttcaatcac cgcctgctga cgaactgggc gcgtagtgga
    45361 cggacgttca gcggtttgcg ttttcgccgt gtcacgcgat gcattgccat tgcgaacagg
    45421 cgcaaccgtt gcgggttcag tcggcaacgt ggaattgacc gccacattgt tcagctgttg
    45481 ctgattttgt ggctgggtca gcgcattgtt caggtcaccc tgcacttcaa cacgttgttg
    45541 accatccgtt gccaccgggg tttgcccttg agtcggcgta gaagagatcg gtggcagaga
    45601 aacatcctgc tgagtatttt ccgcagacgt ggttcccggc gctggctgca cgccattcgc
    45661 ctgatcggtc gcattgccag caagatcaat actcttctcg ccagacgcgg tttgatcgct
    45721 ggaagtggtc gagggggctt ttagcgcaga accgataccg atgatcaaca gcagtagaac
    45781 cagaatgccg acgcccatca tcatatactg acgagaagcg ggtttactgg ctgctttttt
    45841 gcgcttacgc ggacgacgct ctacgcgctc ttcatccacg gtttcatctt cggattcgtc
    45901 aatttcttct tcgatttccg gttcctcatt gcgctctttt tgcgcacgag tcggacggcg
    45961 atcgtcagtg tcatcaagtt caatatcatc aaaattgatc tgcggttcgc cacgttcagt
    46021 acgctcagaa cgttcagaag attgacgaga acgaccagta cgacgatcgc tgggatcggg
    46081 tttcagctcg tcttctggtt tgaattcatc catttaacac cccactaaaa ggttaatgct
    46141 taccacgttg caattaacct gaagctaata gaccgcttga taagcggcct gacctttctt
    46201 gttgttacgc tgattgacaa tcggcaatgg cgttaagaac aagctcgtgc gaaacgccgc
    46261 tgcgaacttc actcttacca attgccaacg gaagaattaa gcgcatctct cccgcaagga
    46321 ctttcttgtc acgcagcata tgcggtaaat acgcctgcgc ggacatttcg cgcggcccat
    46381 tgaccggtaa cccagcccgc gtgagcaggg ttataatacg ctgcgtttcg gcagaactaa
    46441 actgcccgag acgttccgac gtccgcgccg ccatcaccat acccgcagcg accgcttcac
    46501 catgtaacca attgccatac cccatttcag cttcaatggc atgaccaaag gtgtgtccca
    46561 gattcagtaa agcacgtaac ccggtttcgc gctcgtcggc agcgacaact tctgccttca
    46621 gttcacaaca acggcgaata cagtacgcca ttgccggacc gtccagacgc aacaacgcat
    46681 ccagattctc ttccagccag ttaaaaaacg caccgtcaag aataatgccg tatttgatga
    46741 cttctgccag ccccgacgct aactcacgcg ggggaagcgt tttcagacag tcgagatcca
    46801 ccaccactga agcaggctgg tagaacgcgc caatcatgtt tttaccgagg ggatggttga
    46861 ccgcagtttt gccgccaacg gaggaatcga cctgcgacag taacgtcgtc gggacttgaa
    46921 tgaaacgaac accgcgctga taactcgccg ccgcgaagcc ggtcagatcg cccactacgc
    46981 cgccgccaag cgccaccagc gtagtatcgc gaccatgcgg cttttgtaac aacgccgtaa
    47041 agacggtatc gagtacagct aggcttttat actgctcgcc gtcagggagg ataacgctat
    47101 cgacgttaac acccgcctgt tcaagtacgc cgcggacctt atcgagatac agaggagcca
    47161 gggtttcgct ggtgaccaac atgacctgct cgcccgattt cagcggtaag aatgaagctg
    47221 gttcattaaa caaaccagat gcgatggtaa ttgggtaact acgttccccg agagtaacga
    47281 caatcctctc cataacgcga catccacctt aattactgta cccgcagacg agtgtatata
    47341 aagccagaat tagttgcttt ccagcatgtg aataatctgg tttgcaacca ctttagcgct
    47401 ttgatcatca gtacgaatgg tcacgtcggc aatctcttca tacagcggat tgcgttcatt
    47461 ggccaacgct tccagaactt cacgcggcgg tgtttcaacg tgcagcaacg ggcgtttttt
    47521 atcacgctgc gtgcgtgcaa gttgcttttc gatggtcgtt tcaagataaa cgacaacgcc
    47581 acgagcggaa agacggttac gcgtttcacg ggatttcaca gagccgccgc cagtagccag
    47641 cacaataccc tgtttctcgg tcaactcatt gatgaccttt tcttcgcgat cgcggaagcc
    47701 ttcttcgcct tctaaatcga aaacccagcc cacatcagct ccggttcgtt tctcaatctc
    47761 ttgatcggaa tcgtaaaatt ccatattgag tttttgagct aactggcgcc caatagtgct
    47821 ttttccggca cccataggcc caaccagaaa gatattgcgt ttctctgcca ttttttcggt
    47881 actactaaga ctattcgtta atgataaacc cgcttcgctc agagagcgcc gcaggacatg
    47941 aactgaaacc tcataagata ttgcgagagt cagactgaaa attatctcaa tactccagcg
    48001 ggtttggcaa ctgaataaat cacccatccc tctgcatatc tggtcgctgc aagcgcactg
    48061 ccttgctacc accgctctgg cgataaatca ccgggtaaga ttagcgtaaa aaagacagca
    48121 aaatgccgcc tgaatgataa atcatcatca tggaacgcag gcagcgaaga gcaccaactc
    48181 tcaaatcggt actccttgta tgctaaatac ctgcgcgcgt caaatagatg aaacacgttc
    48241 agcataaaaa cattaccgct tttacggctg tttactcact ggaaaccagt cgtggcgtga
    48301 taaacaccac taactcgcgt cgttcatctt cttttccgtc atgacgaaat aattgcccga
    48361 accagggaat gtcgccaagc aacggtacgc tatcctgacc cgatttattt ttacgggtaa
    48421 aaatgccgcc cagcgccaac gtttctccgc tttttacctc gacctgcgtt tcgatctcct
    48481 gcttatcaat cgccagcact tcgccatcgg cctgctgtag cacctgcccc ggaacgttct
    48541 ggctgatgtg taatttcagc cggatgcgac ctttttgtaa caccgtgggc gtgacctcca
    48601 tccccaggac ggcctcttta aattccaccg acgtcgcgcc actttccccg ctggaaacct
    48661 gatatggaat ttcgctcccc tgtttaatgc tggcaggctg aagatgtgag gccagcagac
    48721 gcggactggc gataatatcc agctgctgtt tttgttcgag cgcggaaagc tcaagatcca
    48781 gcaagcgtcc gttgatgcgc ccaatgttaa aaccgacatg cgttgtcgcc gtcgctacgg
    48841 agaggtcgct accaagcgtg gtgacttgcc caacgccacc agcgtgttgc gcatcggcca
    48901 gcgtccattt cacgcctaac tcacgcaaac ttttttcatt aatggtgaca atatgcgccg
    48961 acagctcaac ctgcccgacc ggcagatcca tttgcgctac ccactgttca agcgcgctta
    49021 acgccgtttt gttatctcgt agcaaaaggc gattggtgcg tttatcgacg gtcatactcc
    49081 ctttggcact cagtagcttc tcccccgctt tcgccagttc tcccgcgtcg gcgtattgca
    49141 gggttatact gcgattttcc agcggcagat ttgcctgcgc ccgcgcctgc tccgcctcct
    49201 ggcgggcgat attgtcattc tgccaggcaa tggaatgcac tgagagaatg ttgccttcct
    49261 gccgcgttat cagtccggcg cttttcacta cagtttgtag tgcctgcttc cagggaacat
    49321 ctgttagatg taacgacacc gtaccgctga cgtctggcga cacgaccagg ttcaacttct
    49381 cctgttcagc cagcgcctgc aacacctgag ctaccggaac gtcatccacc atcagcgtca
    49441 ctttttgcgg ctttgccgcc tgtacgccgg atatcagcat caacagtagt gcggctatcc
    49501 attgcttcat ttgtatctcc ttgccgttgc cacaaccatt gtggcggttc gcagtttgtc
    49561 ccggtaccca gcgttagtac gtctggcgtc agctgtaaaa ttgtccagcc gttttccagc
    49621 acatcgtttt gctgcacccg tcgccatttc ttttgcccgt cttttattac accgatgatg
    49681 cgctcgcctc gccctaccat cccctgatag cgccactggc taagttcgct aatccggcat
    49741 agatcttccg gcgatttaaa agggtcacgc ataccggtta aaaggcacaa tgcaatacct
    49801 gccaacaacc agcgtttaac cctcatgcgg catctccagt tgtagcgtga acaaaagatc
    49861 atcaccttcc acgcttaacg aaaaacggct cacgctgacg ttgcgctctg ccagccgtgt
    49921 aaatgccgac ggcactgctt cccacagcgt tttcaacgcc aactcgcctc cctgcgcgga
    49981 tggatgccag gaaaccagtt gcgcgccgga taactgaaaa tccagtggcg aaaagggcag
    50041 cgttttttcc tcgctgaagg gagtggtgtc taccaggcga tacagtgcgg cccactgctg
    50101 gtgatgactt gcccgtagtc gaattaatgc gtcgcgctct tcatggtgtg tcgatgatag
    50161 aaaaatgagc gtaactaaca tcagcaacca gaatgcccag caaaactggc ggaggcgggg
    50221 tgatgtggcg aaccaccagt caaagaacat gttcatcgct aaccttcctt gttaactgat
    50281 actcaaattg ccagcgtccc tgcgcatcct gctgcgtggc tccccgctga ttgagatgaa
    50341 aagacgcatc ctggcggagt gacgtttcta gtgcgtttaa cgcggtaatg cttgttgtca
    50401 gccccttgat ctccagcgtt ccctgctgcc agcttatcgt tgtcagccag gcgtgctcgg
    50461 gtaaaagcgc cgccagtgct tccagcgcag attgccagtc gcgggtaaat tgtcgctggc
    50521 gctgccgttg cgaacgctgt tcgcgtaact gctgttgctc cagcaaacgt ggcttcgtta
    50581 tctgtaagct gcgggcgagt tgttgttccg cctgaagcaa aactgcgtct atgcgcgctt
    50641 cggcgctgcc tgtcagacgc agtattagcg ttatcccgac ggccagcagc agaggcgcaa
    50701 cgaacatcag caaccagaaa cgcagaaaag cggtccggcg ttgctgtcgc cagggcaaaa
    50761 aattaattgg cgggttcatc agtacgcctt cccaagcgcc agccccagcg cgatggcaaa
    50821 gtctccacca ggcggcggta gcggcggctg acgaacagaa acggcctccc aggggtcaaa
    50881 tccgccttcg ccacatatcg cgacgctgtc gggatcaacg gataacgctg ccgccagctc
    50941 cttcgcgcta gtcatcccca ccgccagttt gcgcccccag ctatagcgcg tcgcccacag
    51001 ccactgttcg ttatcacgcc aggccagaca ttgctgatga gaaggtaaaa aaggcaggaa
    51061 tcgctgtaat gcactggcat ccggggtgat cgcactaaca tgaacacgca acctttctgc
    51121 cagcgtaagc agcgttgcca gctctttgct ttgcgcggca gtcacgttat aagcggggct
    51181 gagtgagtct tcgctgtaat cgaagcgcag ggagtccgga tccatatcca gctcgcgggc
    51241 catcgtccct gacagccagg ccgtttgctc ccgctcacca agggacatcg acgggcgcgg
    51301 aaatgaccgc tgtaatgtgc gactggcggg aaacgccaac ataatgtgat gacgctgcgg
    51361 cagttcgcga ctccacggta acaacgtttt agccagctgc tgcgcatcaa caatccgccc
    51421 atctttgata atgtcgtgcg ccagcggcaa ccgccaccag cgttgcaaaa agcattcttt
    51481 tgcgccccgc acgatcgcaa ccgctgccac ttcttgctgt tgtaaatgca aaccaatttg
    51541 ccagatctta aatgtcattg tgatgatctc cttatcaccc gtcactctga cgggtatatc
    51601 aatgcgtctg gcttgccttt atactaccgc gcgtttgttt ataaactgcc caaatgaaac
    51661 taaatgggaa atttccagtg aagttcgtaa agtatttttt gatccttgca gtctgttgca
    51721 ttctgctggg agcaggctcg atttatggcc tataccgcta catcgagcca caactgccgg
    51781 atgtggtgac attaaaagat gttcgcctgc aaattccgat gcagatttac agcgccgatg
    51841 gcgagctgat tgctcaatac ggtgagaaac gtcgtattcc ggttacgttg gatcaaatcc
    51901 caccggagat ggtgaaagcc tttatcgcga cggaagacag ccgcttctac gagcatcacg
    51961 gcgttgaccc ggtggggatc ttccgtgcgg caagtgtggc gctgttctcc ggccacgcgt
    52021 cacaaggggc gagtaccatt acccagcagc tggcgagaaa tttcttcctc agtccagaac
    52081 gcacgctgat gcgtaagatt aaggaagtct tcctcgcgat tcgcattgaa cagttgctga
    52141 cgaaagacga aatcctcgag ctttatctga acaagattta ccttggttac cgcgcctatg
    52201 gtgtcggtgc tgcggcacaa gtctatttcg gtaaaacggt cgaccaactg acgctgaacg
    52261 aaatggcggt gatagccggg ttgccgaaag cgccttccac ctttaatccg ctctactcaa
    52321 tggatcgtgc cgtcgcgcgg cgtaacgtcg tactgtcgcg gatgctggat gaagggtata
    52381 tcacccagca acagttcgat cagacacgca ctgaggcgat taacgctaat tatcacgcgc
    52441 cggagattgc tttctctgcg ccgtacctga gcgaaatggt gcgccaggag atgtataacc
    52501 gttatggcga aagtgcctat gaagacggtt atcgcattta caccaccatc acccgcaaag
    52561 tgcagcaggc cgcgcagcag gcggtacgta ataacgtgct ggactacgac atgcgccacg
    52621 gctatcgcgg cccggcaaat gtgctgtgga aagtgggcga gtcggcgtgg gataacaaca
    52681 agattaccga tacgctgaag gcgctgccga cctatggtcc gctgctgcct gccgcagtca
    52741 ccagcgccaa tcctcaggga gcgacggcga tgctggcgga cgggtcgacc gtcgcattga
    52801 gtatggaagg cgttcgctgg gcgcgtcctt accgttcgga tactcagcag ggaccgacgc
    52861 cgcgtaaagt gaccgatgtt ctgcaaacgg gtcagcaaat ctgggttcgt caggttggcg
    52921 atgcatggtg gctggcacaa gtgccggaag tgaactcggc gctggtgtcg atcaatccgc
    52981 aaaatggtgc cgttatggcg ctggtcggtg gctttgattt caatcagagc aagtttaacc
    53041 gcgccaccca ggcactgcgt caggtgggtt ccaacatcaa accgttcctc tacaccgcgg
    53101 cgatggataa aggtctgacg ctggcaagta tgttgaacga tgtgccaatt tctcgctggg
    53161 atgcaggtgc cggttctgac tggcagccga agaactcacc accgcagtat gctggtccaa
    53221 ttcgcttacg tcaggggctg ggtcagtcga aaaacgtggt gatggtacgt gcaatgcggg
    53281 cgatgggcgt cgactacgct gcagaatatc tgcaacgctt cggcttcccg gcgcaaaaca
    53341 ttgtccacac cgaatcgctg gcgctgggtt cagcgtcctt caccccaatg caggtggcgc
    53401 gcggctacgc ggtcatggcg aacggcggct tcctagtgga cccgtggttt atcagcaaaa
    53461 ttgaaaacga tcagggcggc gtgattttcg aagcgaaacc gaaagtagcc tgcccggaat
    53521 gcgatattcc agtgatttac ggtgatacgc agaaatcgaa cgtgctggaa aataacgatg
    53581 ttgaagatgt cgctatctcc cgcgagcagc agaatgtttc tgtaccaatg ccgcagctgg
    53641 agcaggcaaa tcaggcgtta gtggcgaaga ctggcgcgca ggagtacgca ccgcacgtca
    53701 tcaacactcc gctggcattc ctgattaaga gtgctttgaa caccaatatc tttggtgaac
    53761 caggctggca gggtaccggc tggcgtgcag gtcgtgattt gcagcgtcgc gatatcggcg
    53821 ggaaaaccgg taccactaac agttcgaaag atgcgtggtt ctcgggttac ggtccgggcg
    53881 ttgtgacctc ggtctggatt ggctttgatg atcaccgtcg taatctcggt catacaacgg
    53941 cttccggagc gattaaagat cagatctcag gttacgaagg cggtgccaag agtgcccagc
    54001 ctgcatggga cgcttatatg aaagccgttc ttgaaggtgt gccggagcag ccgctgacgc
    54061 cgcctccggg tattgtgacg gtgaatatcg atcgcagcac cgggcagtta gctaatggtg
    54121 gcaacagccg cgaagagtat ttcatcgaag gtacgcagcc gacacaacag gcggtgcacg
    54181 aggtgggaac gaccattatc gataatggtg aggcacagga attgttctga ttaaaaaggc
    54241 gcttcggcgc cttttcagtt ggctgacaaa gtgcatttgt ttatgccgga tgcgggtgaa
    54301 cgcgttatcc ggctaacaaa atcgtgaaaa atcaatatat tgcaggaacc ccataagcct
    54361 gataagcgtt gtgcatcagg caaacttcac gcatttacac tcgcccctgc cctttcaacc
    54421 attcgcgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca gggtcttcca
    54481 gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct ggctcatcgc
    54541 cttccagcga ttccgggtag agatcttgcg cgaccacgat attcatttta ctggaaaagt
    54601 aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca aatccaacct
    54661 cttcttttag ctcgcggtta gcggcttcgt agacgctttc acccggatca attaatcctt
    54721 tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc aggatcaggt
    54781 gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc attcgttcat
    54841 aaacacgccg cacgccattg ctgaactcca gatccacact ctcgacggta aacagtcggg
    54901 aacgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg ttcatcgtgg
    54961 gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc ggcaatgtga
    55021 attgcatgtt atttacattt atgtaactta ataaataatc gtcctcaaat caaattaaaa
    55081 gtcaataggt tgaaataact ccaggaattt gctgatattc cgccttcaga gggtttgcta
    55141 tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taaactccac gcttgccgat
    55201 agccaaccgc agaatcatgt attgtgtccg gtgcgactga ccacgcctga cagactaagt
    55261 aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg gcctgctcac
    55321 tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc tggaccaacg
    55381 ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc gttgaaaatt
    55441 atcttgagag cctgacgcag gtattacagg tgcctggccc aacgggagct agcgcggcac
    55501 cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca cacgctatca
    55561 cacgttacgg catctctacc gacgatccga ataaatggcg ttactacctc gattcggtag
    55621 aagtccacct gccccccttc tgggaacagt acatcaacga tgagaatacc gttgaactga
    55681 ttcataccga ttcgctgccg ttggttattt cactcaacgg tcatacgctg caggagtaca
    55741 tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc cacgcaggcg tcgattcgcg
    55801 gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcat gaagaatatg
    55861 cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat cgtcgcctcc ttcctgatgt
    55921 tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc ggcgcattac
    55981 tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc tccctgcgag
    56041 aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa aacgatcagg
    56101 aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca cactggcagc
    56161 catacattgc tcaggatctc ggtcaacaaa ccgatattga tatctatcta gaccgccacg
    56221 tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt ccgttacagc
    56281 actggctgcg cagtacgatt atcgctgcgg gctcgctgct ggtgctgttt atgctgttat
    56341 tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa ggcgcgcaga
    56401 ccattgaagc caccagcgta aaacaactgg ctgacgctgg cgtgcgggta ggcgatacat
    56461 tgcgtattag cggtacggga atgtgtaata ttcgcacttc cggaacctgg agcgcgaaaa
    56521 ccaattcacc ttttttacca tttgactgct cgcagatcat ctggaatgac gcccgctcat
    56581 taccattacc agaatctgaa ctggtcaaca aagcgacggc attgactgaa gcagttaatc
    56641 gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag tgcctcatta cgttcagcga
    56701 ttcaaaaatc cggcatggta ttgcttgatg attttggcga cattgtactg aagacagcgg
    56761 atttatgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc aatctcggca
    56821 acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc tgggaagctc gatggcgtga
    56881 atgtgttatt acgcccggtg agtgcggaat cgctggataa cctggtggca acctccaccg
    56941 cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt ccggcccccg
    57001 gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc tggccttcgg
    57061 catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa ctggcacaaa
    57121 tgctgatgca tacgccgttt aacgccgaag gtatcgtcac aaaaatcttc actgacgcca
    57181 atggtacgca gcatattggc cttcatccga tcccggatcg ttccggcctg tggcgctatc
    57241 tcagcaccac attgctgcta ctgacgatgc tgggtagcgc catttacaat ggcgtacagg
    57301 cctggcgtcg ttaccagcgt catcgcactc gcatgatgga gattcaggcc tattatgaaa
    57361 gctgcctgaa cccgcaactg atcacccctt cagaaagcct tatcgaataa cacgtttgcg
    57421 cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc cctcatgcat
    57481 atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg cacgttgctc
    57541 gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg gggcgcgaaa
    57601 aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca cgacgtacag
    57661 catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct ggatatctgt
    57721 gcgatgacca ccgagatggg accgcgcgcc gtactgcgtg aagataccat tccgtttctt
    57781 gaggcactga aagccagcgg taagcagcga attttgctca ccaatgctca tccgcacaac
    57841 ctggcggtaa aacttgagca tacgggtctg gacgcacacc ttgatttatt actttccacc
    57901 cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc cgaagctacg
    57961 ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct cgatgctgcc
    58021 gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgattccgg gattgccgag
    58081 aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc ctcgctaatg
    58141 tgaaggagac gccatgaaag agaaacctgc tgttgaggtt cgactggata aatggctatg
    58201 ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattaaag gcggtaaggt
    58261 gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg ccacgctcac
    58321 tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg aacagcgtcg
    58381 ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag agaaacgcga
    58441 aaaaatggcg atggcacgta aacttaatgc cttaaccatg ccgcacccgg accgacgccc
    58501 ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg aataactgtc
    58561 acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc tgtttgaaaa
    58621 ttttgccgtt cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga tccttgagaa
    58681 ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg cgaccagcct
    58741 gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcagg gcgacggtcc
    58801 gatgaatctg gcggttatta acggtaacaa taaccagcaa atgcgcggtg tggcgcgcgt
    58861 gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg gttacgtggt
    58921 gatcaccatt accccgagcg aaggcgaacg ctatcagggc gtagttggtc tggaaggtga
    58981 taccctggcg gcctgcctag aagattactt tatgcgttct gaacagctgc cgacgcgcct
    59041 gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt tgttgcaggt
    59101 aatgcctgcg caaaataccc agcaggacga ctttgaccac ctggcgacgc taaccgaaac
    59161 catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc gtttgtatca
    59221 cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca cctgctcgcg
    59281 tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa gaagttgata gcatcctggc
    59341 ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc tgttcaatgc
    59401 gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgcaag ttcattaatg
    59461 agtatgtccg gcagagaggg ctctgccgga ttttacgact taatggcctg atgtgctgcg
    59521 cttaccagcg cctatgaaat aaatcgcaaa ttattttggt cagatacagc gtcgtatccg
    59581 ataaaagcgt tgtcacttac ttaagtttat ttccagcgtt tcaccatcta ccgtcagatc
    59641 gcgccacgct ttcggcctcg tccccagttt tccatccttc gcagctgtga gtaatttttc
    59701 tttcgttatt tcgttcggaa gtaaactcat atcaagcgcg tcccattctt ttctgtctga
    59761 gtcaaagcca tagacaatga ctctgtcatc attaaaagca aacaggatcc gctccgcctg
    59821 accatcacta ttcagatctt gctcgaccag tacgcaggcg tctttttcaa tacaggtcat
    59881 cacgttatag cgatcctgga ttaaggccga ccagaatgtc gcatccggtt taacagaacc
    59941 aggggcaatt aacacatttt cggctaatac tttttccgat acctgttcct gaagatgttg
    60001 ctctccatct aacgccatca gcagatcccg cgcgcgtttc gggtctttca taaacccggc
    60061 atcgcttttc agcgactcaa gcgccgcacg tccatagcga ccgctctgtt cgagcatgta
    60121 gatagttacc tggtctggcg tgtttttgcc gctctgataa cgcgccatat gactgttcac
    60181 gctaatacgc atactgtcca gcaccggcga attaagaagc accagtatca ccaacaccaa
    60241 taacgaaacc gcaaggttca ctttgccctg aagaacaagg ggattttgcc cattacgcca
    60301 gacgatactg acaaaatacc ccagcgacca caccagtaac accagctccg ccagcgcgcc
    60361 ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc agccataacc cccaggtggc
    60421 gacgaacaca tacagcggag caaccagcaa agcggtttta atcaggcaac gcaacggccc
    60481 cgtccaggga agtgacgctt tttgcggatc gcggacaata gccattaaga tcaattgcaa
    60541 aaaggccagc gttaacagca acccggcggc ggagatgtgg cgagaaatcg cgctcaggcc
    60601 cgtaaacggc agggtgatga taaacatcag ggttagcaat gatactaacg gcagcaaccc
    60661 cgtggcgatt agcgtgaaca acttttgaat agagtcgatt aaacgtgact gtgtacgcgc
    60721 gaggatcacc gccagtgcgg taaccagacc aaatgtaaga tacataaacc agtcggttgc
    60781 aaaaaagagc gtattgaaaa acgtgatgcc aacaagttta aacaactcac cccacagtaa
    60841 aagcaccagc cacgtcaggc cattggagag aaaaatcacc agtaatataa gtacgttatg
    60901 ccatactgac tggtaaaagt agctatagcg gctactgcca ttgcggatac gaaggctttg
    60961 ttgtatccac ggtagcaaca acattgccat caacagcaga tagcagccaa aatcccaaat
    61021 cgccttttca gctctccagg ggttcatgcc atcagtttgc cacttcagcc aaccgctcat
    61081 ccctgtcgtg gcaataaaca ccagcgccag ccatccccaa aggcgcttct gtttaaaaga
    61141 gatcacggag aagagtaaaa cggatgaaaa cgcgacggtc gcaggcacgc cataaacaat
    61201 ccaactgtga ttttttccgg caagccaggc gataagcagg tagcaaacca gcccctgaag
    61261 taatccggta gcaatcatcc cccagcgtgt tgccggtgaa agttcaacgt tatccatgtt
    61321 ggttatccag aatcaaaagg tgggttaatt atcgcatccg ggcagtagta ttttgctttt
    61381 ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat actttaccgg ttgaatttgc
    61441 atcaatttca ttcaggaatg cgattccact cacaatattc ccgccatata aaccaagatt
    61501 taaccttttg agaacatttt ccacacctaa aatgctattt ctgcgataat agcaaccgtt
    61561 tcgtgacagg aatcacggag ttttttgtca aatatgaatt tctccagata cgtaaatcta
    61621 tgagccttgt cgcggttaac acccccaaaa agactttact attcaggcaa tacatattgg
    61681 ctaaggagca gtgaaatgcg cgttaacaat ggtttgaccc cgcaagaact cgaggcttat
    61741 ggtatcagtg acgtacatga tatcgtttac aacccaagct acgacctgct gtatcaggaa
    61801 gagctcgatc cgagcctgac aggttatgag cgcggggtgt taactaatct gggtgccgtt
    61861 gccgtcgata ccgggatctt caccggtcgt tcaccaaaag ataagtatat cgtccgtgac
    61921 gataccactc gcgatacttt ctggtgggca gacaaaggca aaggtaagaa cgacaacaaa
    61981 cctctctctc cggaaacctg gcagcatctg aaaggcctgg tgaccaggca gctttccggc
    62041 aaacgtctgt tcgttgtcga cgctttctgt ggtgcgaacc cggatactcg tctttccgtc
    62101 cgtttcatca ccgaagtggc ctggcaggcg cattttgtca aaaacatgtt tattcgcccg
    62161 agcgatgaag agctggcagg tttcaaacca gactttatcg ttatgaacgg cgcgaagtgc
    62221 actaacccgc agtggaaaga acagggtctg aactccgaaa acttcgtggc gtttaacctg
    62281 accgagcgca tgcagctgat tggcggcacc tggtacggcg gcgaaatgaa gaaagggatg
    62341 ttctcgatga tgaactacct gctgccgctg aaaggtatcg cttctatgca ctgctccgcc
    62401 aacgttggcg agaaaggcga tgttgcggtg ttcttcggcc tttccggcac cggtaaaacc
    62461 accctttcca ccgacccgaa acgccgcctg attggcgatg acgaacacgg ctgggacgat
    62521 gacggcgtgt ttaatttcga aggcggctgc tacgcaaaaa ctatcaagct gtcgaaagaa
    62581 gcggaacctg aaatctacaa cgctatccgt cgtgatgcgc tgctggaaaa cgtcaccgtg
    62641 cgtgaagatg gcactatcga ctttgatgat ggttcaaaaa ccgagaacac ccgcgtttct
    62701 tatccaatct atcacatcga taacatcgtt aagccggttt ccaaagcggg ccacgcgact
    62761 aaggttatct tcctgactgc tgatgctttc ggcgtgttgc cgccggtttc tcgcctgact
    62821 gccgatcaaa cccagtatca cttcctctct ggcttcaccg ccaaactggc cggtactgag
    62881 cgtggcatca ccgaaccgac gccaaccttc tccgcttgct tcggcgcggc gttcctgtcg
    62941 ctgcacccga ctcagtacgc agaagtgctg gtgaaacgta tgcaggcggc gggcgcgcag
    63001 gcttatctgg ttaacactgg ctggaacggc actggcaaac gtatctcgat taaagatacc
    63061 cgcgccatta tcgacgccat cctcaacggt tcactggata atgcagaaac cttcactctg
    63121 ccgatgttta acctggcgat cccaaccgaa ctgccgggcg tagacacgaa gattctcgat
    63181 ccgcgtaaca cctacgcttc tccggaacag tggcaggaaa aagccgaaac cctggcgaaa
    63241 ctgtttatcg acaacttcga taaatacacc gacacccctg cgggtgccgc gctggtagcg
    63301 gctggtccga aactgtaatg atttgaagct ggagaatatc tatccagtat cttatagaaa
    63361 gcaaaacggg aggcaccttc gcctcccgtt tatttaccct tcttttgtcg tgccctgcgc
    63421 ccgcgttacc ggcactggca gccaggcgcg aatggaaagc ccgccccgct cgctggtgcc
    63481 aagctccagc atcccgttat ggttatccac gatacgctgc acaattgcca gccctaatcc
    63541 cgtgccgcta atggtgcgcg cactgtcgcc gcggacaaac ggctggaaca ggtgcttacg
    63601 ttgttccggc gcaattcccg gaccgtcatc ttccacctgg aaccaggcgc gattcggctc
    63661 cgttccgctg ctgactttga tccagccatt gccataacgg gcggcgttga ccaccatatt
    63721 cgccaccgcg cgtttgatcg acagcgggtg cattttcact tcaatgctgc cggggtaaag
    63781 cgcggtttca atttcccgct catagccact ttcggcagca atcacctcac cgagcactgc
    63841 attaagatcc gccatttcca tcggcatctc ctgcccggtg cgcaggtagt cgataaactg
    63901 ctcaatgatg gcgttgcact cttcgatatc tttattgatc gattctgcca gatagccatc
    63961 ctgctcgctc atcatctcag tcgccaggcg aatacgcgtc agcggcgtgc gcaagtcgtg
    64021 acttaccccc gccatcagca gcgtgcggtc atccgccagt tgcttaacac cagccgccat
    64081 atggttaaag gcacgggtaa cggaacgcac ctccgaagcg ccatattcac gcagcggcgg
    64141 cggaataatc cctttaccaa cctgcaaggc tgcgtgttcg agatcgacca acggtcggtt
    64201 ctggatacga ataaacagcc acgccccgcc tatcgccaat agcataatcg ccagcgtata
    64261 gcggaacagc ggagagaaat cgccctgatg aatttcggtc agcggcacgc gtacccagat
    64321 attgggcgac agccaggttt tcagccagac gacaggcgaa cttttgttga cctcaacgcg
    64381 cacttccgtc gggccgccca gttgctgtgc catctgatgg cttaagaatt catagtgttg
    64441 cgcccaacgc agacctgcct cttcggcagc ctcgttggag tagagagaga tccccagctc
    64501 acggtagatc tcccgacgga aagcgggagg cacaaccaac tgcgtgccgt cctccagttg
    64561 cagtttatcg gtcatcaaca tacgcacttc gtacgcgagg actttattaa actgctggag
    64621 gctcggcaaa atcgcgaagt tcagcaccac cagataagtc gtcaccaggc tggcgaacag
    64681 caaggtgacg atgagcaata acgtacgggc aaatgaactt cgtggcgaga agcgcaatcg
    64741 cctcatgctt tagagccgtc cggtacaaag acgtagccca ggccccaaac ggtctgaatg
    64801 taacgcggat gcgctggatc ttcttctacc atgcggcgca gacgcgaaat ctgcacgtcg
    64861 atggagcgtt ccattgcgga atattcacga ccacgggcaa ggttcatcag cttatcgcgg
    64921 gagagcggct cacgcggatg gctgaccagt gccttcagta ccgcaaactc accgctggtg
    64981 agcggcatcg gctcatcttc gcggaacatt tcgcgcgtac cgaggttaag tttgaactta
    65041 ccgaaagcaa ttaccgcctc ttcctgtgac ggtgcgcctg gcagttcgtt cgcctgacga
    65101 cgcagcaccg cacgaatacg ggccagcagt tcacgcgggt taaacggttt tggaatgtag
    65161 tcgtcagcgc caatctccag gcctacgata cggtccactt cttccccttt cgccgtcacc
    65221 ataatgatcg gcatcgggtt gctctgacta cgaagacgtc ggcaaatcga caagccatct
    65281 tcaccaggta acattaaatc cagtaccata agatggaaag attcacgagt cagcaggcga
    65341 tccatctgtt ctgcattagc gacgcttcga acctggaagc cttgttcggt gagataacgt
    65401 tccagcagcg cacgcaggcg catgtcgtca tcgaccacca gaatcttgta gttctcttgc
    65461 attgtttgta ctcccaaagg ttcgcaacaa tttgtaagcg tgtattctta aaaaagctca
    65521 cgttcgtcac cagctaaatc tggtatgaat ttcagcctaa attgttacaa agcatattaa
    65581 acagcagctt aagtatacaa tttattcggc gaaacattat tgattctgtt gatatgatca
    65641 cgttataccc aatgtgcgca ttatcaaaca gacaaaggga atcaacgaga tgaaaacgcc
    65701 cctggttacc cgggaagggt atgaaaaact caaacaagag cttaattatc tctggcgtga
    65761 agaacgcccg gaggtcacaa aaaaggtgac ctgggccgca agtctgggcg accgcagcga
    65821 aaatgctgac tatcagtata ataaaaagcg tctgcgtgaa atcgaccgtc gcgtgcgcta
    65881 tctcactaaa tgcctggaaa atctcaaaat tgtcgattac tcccctcagc aggaaggcaa
    65941 agtctttttt ggcgcgtggg tggagattga aaacgacgat ggcgtgactc accgtttccg
    66001 tattgtcggc tacgatgaaa tttttggccg taaagattac atctctatcg attccccgat
    66061 ggcccgcgca ttgctgaaaa aagaagtcgg cgatctggcg gtggtgaata cccctgccgg
    66121 ggaagcgaac tggtatgtta atgctatcga gtacgtgaaa ccgtaaggaa gagtcttaac
    66181 ctcctgccga tggctggcat ttttgccagc cagtccgtat aactatcccc tgatttttga
    66241 tccgaaaaga tgaactcaaa ccatgatgaa tgattcgttc tgccgcatta ttgcgggtga
    66301 aattcaggcg cgcccggaac aggttgacgc tgccgttcgc ctgcttgatg aagggaatac
    66361 cgtgccgttt atcgcacgtt atcgtaagga aatcaccggc ggtctggatg acacgcagct
    66421 gcgtaatctg gaaacgcgtc tgagctatct gcgcgagctg gaagagagac gtcaggcgat
    66481 cctcaaatcc atttccgagc aaggcaaact caccgatgat ctggcgaagg ccatcaacgc
    66541 caccctaagc aaaaccgaac tcgaagacct ctacctgccc tacaaaccta aacgccgcac
    66601 ccgcgggcaa atcgccattg aagcagggct tgagccgttg gctgacctgc tgtggagcga
    66661 tccgtcacac acgccagaag tcgccgctgc acaatatgtt gatgccgata aaggcgtggc
    66721 agataccaaa gccgcgctgg atggcgcgcg ctatatcctg atggaacggt ttgccgaaga
    66781 tgccgcgctg ctggcgaaag tgcgtgatta tctgtggaag aacgcgcatt tggtttctac
    66841 ggtggtgagc ggtaaagaag aggaaggggc gaaattccgc gactatttcg atcatcacga
    66901 accgttgtcc acggtgcctt ctcaccgcgc gctggcgatg ttccgtgggc gtaacgaagg
    66961 cgtactccag ctttcgctga atgccgatcc gcagttcgat gagccgccca aagagagcta
    67021 ttgcgagcaa atcatcatgg atcaccttgg cctgcgcctg aacaatgccc cggcggatag
    67081 ctggcgcaaa agcgtagtga gctggacctg gcgcatcaag gtgctgatgc atctggaaac
    67141 cgaactgatg ggtaccgtgc gcgaacgcgc ggaagatgaa gcaatcaacg tctttgcccg
    67201 taacctgcac gatctgctga tggcggcccc tgccggactg cgtgcaacga tgggcctcga
    67261 tccgggtctg cgtaccgggg taaaagtggc ggtggtcgat gccactggca aactggtggc
    67321 gaccgatacc atttacccgc acaccggaca agccgcaaaa gcagcgatga ccgttgctgc
    67381 cttgtgtgaa aaacataacg ttgaactggt ggcgatcggc aacggtacag cttcccgcga
    67441 aactgaacgt ttctatctcg acgtgcagaa gcagttcccg aaagtgaccg cacagaaagt
    67501 gatcgtcagc gaagctggcg cgtcggttta ctcagcttcc gagctggcag cgcaggagtt
    67561 cccggatctc gacgtttcgc tgcgtggtgc ggtatctatc gcccgccgtt tgcaggatcc
    67621 gctggcggag ctggtgaaaa tcgatccgaa atctatcggc gtaggtcagt atcagcatga
    67681 cgtcagccag acgcaactgg cccgcaaact ggatgcagta gtagaagact gcgtaaacgg
    67741 cgttggcgtc gatctcaaca ccgcttctgt tccgctatta acccgcgtgg cgggcctgac
    67801 gcgcatgatg gcgcaaaaca tcgtggcctg gcgcgatgag aacggccagt tccagaaccg
    67861 tcagcaactg ctgaaagtca gccgtctggg gccgaaagcc ttcgagcagt gcgcgggctt
    67921 cctgcgcatt aaccacggtg ataacccgct ggacgcctct accgttcatc cggaagccta
    67981 tccggtggtg gaacgcattc tggcagcaac acagcaggca ctgaaagatc tgatgggtaa
    68041 cagcagcgaa ctgcgtaacc tgaaagcgtc tgactttact gatgaaaaat tcggtgtgcc
    68101 gacagtaact gacatcatca aagagctgga aaaaccgggt cgcgatccgc gtccggaatt
    68161 taaaaccgct cagtttgccg atggcgtcga gacaatgaac gacctgcaac cgggtatgat
    68221 cctcgaaggc gcagtgacca acgtcaccaa ctttggcgcg tttgtcgata ttggcgtgca
    68281 tcaggacggc ctggttcaca tctcttcatt gtcgaacaag tttgtggaag atccgcatac
    68341 cgtggtgaaa gcgggcgaca ttgtgaaggt gaaagtgctg gaagtggatc ttcagcgtaa
    68401 acgtatcgcc ctgactatgc gtctggacga gcagcctggc gaaaccaact ctcgtcgcgg
    68461 cggcggtaat gaacgcccgc aaaacaaccg cccggcagcc aaaccacgcg gtcgtgaagc
    68521 gcagcctgcc ggtaatagcg cgatgatgga tgcgctggcg gcagcgatgg gtaaaaaacg
    68581 ttaaacgccc ggactggccc ctaaacctgc gcgataaggc gttcacgccg catctggcaa
    68641 cgaacgcttt gcctgacata aaagtgccgg agaatatctc cggcactttt attccacagc
    68701 caaactcaca atatactccg ttaatattta tcacttcatt aacaactgaa accttaatta
    68761 aacattagcc agtccgggta actcactatt cgaattatat tttcgctgcg atataacctt
    68821 gagccacatc aacattgagt cagattatta ttcaaaccaa cattcgtaca cattttaagt
    68881 attgctgata gaaaccattc tcattatcat tgtgttgttg attatttaat ctctcctttg
    68941 ttggcaaatc atctggtctc atgtcgcggt caaacgcccc atgaggtagt tatccagtta
    69001 atgagaaaca agtaggcacc tatgcaatac actccagata ctgcgtggaa aatcactggc
    69061 ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc tttctctagg catgttacct
    69121 ggctcctctt ttgatgtggt gcgcgtcgct ccactcggcg accccattca tatcgaaacc
    69181 cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct tattagaagt ggaagcggtt
    69241 tcctgttaat acggtgataa caacaatgaa aaaattaacc attggcttaa ttggtaatcc
    69301 aaattctggc aagacaacct tatttaacca gctcactggc gcacgtcagc gtgtaggtaa
    69361 ctgggctggc gttaccgtcg aacgtaaaga agggcaattc tccaccaccg atcatcaggt
    69421 cacgctggtg gacctgcccg gcacctattc tctgaccacc atctcatcgc agacctcgct
    69481 cgatgagcaa atcgcctgtc actacatttt gagtggcgac gccgacctgc tgattaacgt
    69541 ggtggatgcg tctaaccttg agcgtaacct gtacctgacg ctgcaactgc tggaactcgg
    69601 cattccctgc atcgtggcgc tgaacatgct cgacattgcc gagaagcaaa atattcgtat
    69661 tgaaattgat gctctatcgg cacgtcttgg ctgcccggtg atcccgctgg tttcaacccg
    69721 tggtcgcggt attgaagcgc tcaagctggc gattgatcgc tataaagcta acgagaatgt
    69781 ggaactggtg cattacgcac agccgctact caacgaagca gattcactgg caaaagtgat
    69841 gccttccgac atcccgctga aacaacgtcg ctggctgggc ctgcaaatgc tggaaggcga
    69901 tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat ctggatgcca ccctcgcccg
    69961 tctgcgtaat gaaatggatg atccggcgct gcacattgcc gatgcgcgtt accagtgcat
    70021 tgctgccatc tgtgatgtgg taagcaacac cctgacggca gaacccagcc gtttcaccac
    70081 tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg ccgattttcc tctttgtgat
    70141 gtacctgatg ttcctgctgg ctatcaacat cggcggggcg ttacagccgc tgtttgacgt
    70201 cggctccgtg gcgctatttg tgcatggtat tcaatggatt ggctacacgc tccacttccc
    70261 ggactggctg actatcttcc tcgcccaggg catgggtggc ggtattaaca ccgtgctgcc
    70321 actggtgccg cagatcggca tgatgtacct gttcctctcc ttccttgagg actccggcta
    70381 tatggcgcgt gcagcgtttg tgatggaccg cctgatgcag gcgctgggct tgccggggaa
    70441 atcctttgtg ccgctgatcg tcggtttcgg ttgtaacgta ccgtcggtaa tgggcgcacg
    70501 tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg atggcaccgt ttatgtcctg
    70561 cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc ttcttcgggc agaacggtgc
    70621 cctggcggtt ttctcgctgt atatgctcgg tatcgtgatg gcggtgctga ctggcctgat
    70681 gctcaagtac accatcatgc gcggtgaagc aacgccgttt gtcatggagc tgccggtata
    70741 ccatgtacca cacgttaaaa gcctgattat ccagacctgg cagcgtctga agggcttcgt
    70801 tctgcgtgct ggtaaagtga ttatcatcgt cagcattttc ctgagcgctt tcaacagctt
    70861 ctcgttgagc gggaaaatcg tcgataacat caacgactcg gcgctggctt ccgtcagccg
    70921 ggtgataacc cctgtcttca agccgattgg cgtacatgaa gataactggc aggcaacggt
    70981 tggcctgttt acgggcgcaa tggcaaaaga agtggtggtg ggtacgctca acaccctcta
    71041 caccgcagaa aatattcagg acgaagagtt caatccggcg gaattcaacc tcggtgaaga
    71101 gctgttcagt gcggtagatg aaacctggca gagcctgaaa gacaccttca gccttagcgt
    71161 actgatgaac cccattgaag ctagcaaagg cgacggtgaa atgggtaccg gggcgatggg
    71221 cgtgatggat cagaaattcg gtagcgcagc tgccgcatac agctacctga ttttcgtcct
    71281 gctgtatgta ccatgtatct cggtgatggg agccatcgcc cgtgaatcaa gccgtggctg
    71341 gatgggcttc tccatcctgt gggggctgaa tatcgcttac tcactggcaa cattgttcta
    71401 tcaggtcgcc agctacagtc agcacccaac ttacagcctg gtgtgcattc tggcggtaat
    71461 cctgtttaac atcgtagtta tcggtctgct gcgccgcgcg cgtagccggg tggatatcga
    71521 actgctggca acccgcaagt cggtaagcag ttgctgcgca gccagcacca ccggtgattg
    71581 ccattaatgg cttcacttat tcaggtacgt gatttgctgg cgttacgggg ccgtatggaa
    71641 gcgacccaga taagccagac attgaacact ccgcagccaa tgattaacgc catgctgcaa
    71701 cagctggaaa gcatgggcaa agccgtgcgg attcaggaag aacctgacgg ctgcctctct
    71761 ggcagttgta aaagctgccc ggaaggcaaa gcctgtctgc gcgagtggtg ggcgctgcgt
    71821 taaccatact ccatcgcctg ttttgaaaag cccggtatgc gtttgcatcc gggctttttt
    71881 gcgtgcggct ttccataaaa atgcaattct tgcagcgcaa cgtaagttcc tctgaaagcg
    71941 tctcgcaggg atgaaaactc gctaatgcac agatgtggag tggcgcgtag agtcgcggca
    72001 ttcaaacaac aggtgaagga acgccatgag taaaaagcag agttccaccc cacatgatgc
    72061 gctgttcaaa ctctttttac gccaaccgga gacggcacgc gattttcttg cgtttcattt
    72121 accggcaccc attcacgcgc tctgtgatat gaaaaccctc aagctggagt cgagcagctt
    72181 tattgatgac gatctgcgtg aaagctattc cgatgtgctg tggtcggtga aaacggaaca
    72241 aggaccagga tacatctatt gtctgattga acatcaaagc acctcaaaca aactgatcgc
    72301 atttcgcatg atgcgttacg ctattgccgc aatgcaaaat caccttgatg ctggatacaa
    72361 aacgttgccg atggtggtgc cattgttgtt ttaccacggt attgaaagcc cctatcccta
    72421 ttcgctgtgt tggctggatt gtttcgccga tcccaaactg gcaaggcagc tttatgcctc
    72481 cgcatttccg ctgattgatg tcaccgtcat gcctgatgat gaaatcatgc agcaccgacg
    72541 tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa cgcgatctga tggggctggt
    72601 agagcaaatg gcctgcttat taagtagtgg atacgctaat gacagacaaa tcaaagggct
    72661 gtttaattac atactgcaaa ctggcgacgc tgtacgtttt aacgatttta tcgacggcgt
    72721 tgccgaacgt tcaccgaaac acaaggagag tttaatgact attgcggaaa gattgcggca
    72781 ggagggggaa caatccaaag ccctgcatat agccaaaata atgcttgaat ccggagtccc
    72841 tcttgcagac atcatgcgat ttaccgggct gtcagaagaa gagttggctg cggcgagtca
    72901 gtagatactc taccctgctt aagtgctact ttgtgctatc ttgcatgtaa cacaaagtag
    72961 cacttaggga actgatatgc agacagtgaa attacggcaa caaggaggag ccatgatcgt
    73021 tactatccca cgagatcttg ccatcgatct tggctggtca ccgggcacag agcttaccgt
    73081 ggagaaaaaa ggcgatagcg ttaatttgcg cgccacagag cacaaaccgc gtgggcgtct
    73141 taccgtcgcg cagttgttga gccaaatcga tgaaagcgaa attacggagc taaatcagtc
    73201 cacagaagga tgggctgaag ggaaaaaagg taatgaagca tggtaaaggc acggacgcca
    73261 catcgtggtg agatctggta ttttaaccct gatccggttg ccgggcatga acttcagggg
    73321 ccacattatt gcattgtggt aacggacaaa aaactcaaca atgttttaaa agttgctatg
    73381 tgctgcccga tttcaacagg ggcaaatgca gcacgttcca caggggtgac ggtgaacgtc
    73441 ctcccctgtg atacgcaaac cggtaacctg catggcgttg tactttgtca ccagctaaaa
    73501 gccgtcgatc ttattgcccg tggcgctaaa tttcataccg ttgccgatga aaaattgatt
    73561 agtgaagtta tcagtaaact ggtgaattta atcgacccac aataattagc tctcacttac
    73621 caccaacacc ctctgcttca acgccaccag caggtgacaa aactcgaccg gatgcgaaat
    73681 aaacggcgca tgggccgctt tggcgaagat atatgattcg ctgtgaggcc aaagtttatc
    73741 cagcatcggc accactttgc gcggcaccag accgtcgaga tagccataca atcgcaaaaa
    73801 cggcatggac acgttttgca gcggctgacg gagatcgacc gttttcagga tttccagccc
    73861 gccattaagc acgtcaacct ccggcatcgg taacgccaga acggttttct tcaacgcccg
    73921 cgcatcctgg cgcgccgttt cagtccccat ggtttgtaac gccaggaacc gctccactgt
    73981 acgctgaaaa tcatcactga gttgctgctg aaatcccgcc agcacgtccg gttttatccc
    74041 cggccactcg tcacgagcac taaaataagg tgacgacgcc acggtgacca gcgcctgaac
    74101 acgctcggga tgggttaacg caatctggct tgccaccagc ccgcccagac tccagcctaa
    74161 ccaaatggct ttatcaggtg cctgttgcag cacggcttcg gccatatcag caagtgacag
    74221 cgcaccaaat ccccggctac gcccgaagcc gggcaggtca acaaggtgca gcgtaaaatg
    74281 cgagctaagt tcctcgtcaa tgcaacgcca cacttcggca ttcagtcccc atccgtgcag
    74341 cagcacaaga tgaacattcc cctgaccttt ggtctgccac cagatgttat tcatccgcta
    74401 ttgttctctt ttgacttaca aggatgaaca tatgctaaca gtaccgggat tatgctggct
    74461 atgccgaatg ccactggcgt taggtcattg ggggatttgt tcggtctgtt cacgcgccac
    74521 ccgcacagat aaaacgttat gcccacaatg tggattaccc gccacacact cccatcttcc
    74581 ctgcggtcgc tgcctgcaaa aaccgccgcc ctggcaaaga ctggtcacgg ttgccgacta
    74641 tgcgccgccg ttaagtccgc ttatccacca gcttaaattt tcccggcgca gtgaaatcgc
    74701 cagcgccctg tcacgtctgt tactattgga agtcttacac gctcgtcgca ccaccgggtt
    74761 gcaattgccg gatcgcatca tcagcgttcc gttatggcag cggcgtcact ggcgtcgggg
    74821 atttaatcag agcgatttgc tgtgtcagcc gttatcacgc tggttgcact gccaatggga
    74881 tagcgaagcc gtcacacgta cacgggccac tgcgacccag cattttctca gtgcccggct
    74941 gcgcaagcgc aacctgaaaa atgcctttcg tcttgaattg cccgtgcaag gtcgccatat
    75001 ggtgattgtg gatgatgtcg ttaccaccgg aagtaccgtc gcagagattg cgcagttgct
    75061 tttacgcaat ggtgcggcga ctgtccaggt ctggtgcctt tgtcgaacct tgtagagcct
    75121 cgatgatggg cgtattataa ccaactaaaa tagtcaacta ttaggccatt actatgatcc
    75181 gtatttccga tgctgcacaa gcgcactttg ccaaactgct ggcaaatcag gaagaaggga
    75241 cacaaatccg cgtatttgtg attaaccctg gcacgcctaa cgctgaatgt ggtgtttctt
    75301 attgtccgcc ggatgccgtg gaagccaccg acactgccct gaaatttgac ctgctgaccg
    75361 cgtatgttga tgagttaagc gcaccatacc tggaagatgc agagatcgac tttgttaccg
    75421 accagttggg ttctcagtta acgctgaaag ccccgaacgc caaaatgcgt aaagtggcag
    75481 acgatgcacc gctgatggag cgcgtggagt atatgctgca gtcgcagatc aacccacagc
    75541 ttgctggtca cggtggtcgc gtttcgctga tggaaatcac cgaagacggt tacgccattc
    75601 tgcaatttgg cggcggctgt aacggttgtt ccatggtcga tgtgacgctg aaagaaggga
    75661 tcgagaagca gctgctgaac gaattcccgg agctgaaagg tgtgcgcgat ctcaccgaac
    75721 accagcgcgg cgaacactcc tactactaag ttatcccctc ttttgtagat tgcccgatgc
    75781 gacgctaaag cgtcatttcg ggcctacgtt gaacatgtgc cagacgttgg cacagcattg
    75841 ctttggcaaa tccctccctt cgtttccccg cgataatatg accaacctct cataatttaa
    75901 atttaccccg ctctggtgat tctcaaacgc cagatgttac ccgtatcatt cacatgggta
    75961 ccaaacatac tcctgacatc tgactacaat aattagtttt agtgggtatc agtcgtggtg
    76021 ccgcaatatc tctgttcccg attgggataa ttagagtttg tcgtcagaaa attgacatta
    76081 cccataacaa atgaaaggcc aggtaaatca tgccattagt cattgttgct atcggtgtaa
    76141 tcttgttgtt gctcctgatg atccgcttca aaatgaacgg cttcatcgct ctcgtcctcg
    76201 tggcgcttgc tgttggatta atgcaaggaa tgccgctgga taaagttatt ggctccatca
    76261 aagccggtgt cggcgggacg ctcggtagcc ttgccctgat catgggtttt ggcgcaatgc
    76321 tggcaaactg cggtggcgca caacgtatcg ccaccacgct gattgccaaa tttggtaaaa
    76381 agcacatcca gtgggcggtg gtgttaaccg gttttaccgt tggttttgcc ctgttctatg
    76441 aagtgggctt cgtgctgatg ctgccgctgg tgtttaccat cgcggcttcc gcgaatattc
    76501 cattgctgta tgttggtgta ccaatggcgg ctgcactgtc tgtgacccac ggcttcctgc
    76561 caccgcatcc gggcccgact gcgattgcca ccattttcaa tgccgatatg ggtaaaaccc
    76621 tgctgtacgg tactattctg gcaatcccga ccgtgattct cgccggtccg gtttacgctc
    76681 gcgtgttgaa aggtatcgat aagccaatcc cggaaggtct ctacagcgcg aaaaccttca
    76741 gcgaagaaga gatgccgagc tttggcgtca gcgtctggac ctctctggtg ccggttgtac
    76801 tgatggcgat gcgtgcgatt gccgaaatga tcctgccgaa aggtcacgct ttcctgccgg
    76861 tagcggagtt cctcggtgac ccggtaatgg caacgctgat tgccgtgctg attgcgatgt
    76921 tcacctttgg tctgaaccgt ggtcgttcaa tggatcagat taacgacacg ctggtttctt
    76981 ccatcaaaat cattgcgatg atgctgttga tcatcggtgg tggcggtgcg ttcaagcagg
    77041 tgctggtaga cagcggcgtg gacaaataca ttgcttccat gatgcacgaa accaacattt
    77101 ctccgctgct gatggcctgg tcgattgctg ccgtactgcg tatcgcgctg ggttccgcaa
    77161 ccgttgcggc aatcactgcg ggtggtatcg cggcaccgct gattgcaacg acgggtgtta
    77221 gcccagagct gatggttatt gcggtgggtt ccggtagtgt gattttctct catgtgaacg
    77281 atccgggctt ctggctgttc aaagagtact ttaacctgac tatcggcgag accatcaaat
    77341 cctggtcgat gctggaaacc attatctcgg tgtgcggtct ggtaggctgt ctgctgctga
    77401 atatggtgat ttgataacac tgactgccgg atgcatcgca tccgacatca accgcactct
    77461 acttcttctt cgctgcagct ctgcgccgtc tgtccaaatc cttcagcaac ttgttcacgc
    77521 catcatcggc aaacatcgac tcaagcgttg cggaaagctt gcgtcgccag tttttatact
    77581 ggtaactggt gccaggaata ttcaccggtt cggccatatc cagccagtct tccggctgta
    77641 gtcctaacag agcactgtta ctgtcggcaa tgtagcgctg caaaccacgg ttcagcgtcg
    77701 tcgtcatcga catcaacgat gccttatgcc cggcacgttt tggcagacaa ccatatttat
    77761 gcagtgcatc cagcagccct tgcttcgcca gttcgcgatc ctgatacaga ccgcgcagta
    77821 ccacttcatc cggatacagc cccagggttt tgcccagcgt tagatccccg ctctcccagt
    77881 aaccacgcag cgttggcagg tcatgtgtcg ccgcaaccgc catcgactgc tccggatacg
    77941 ctttcggtgc acggaacgtc ttctcgtggt cgttttcgaa atagagcact ttgtaagagt
    78001 acacaccgct gctgcgcagc ttaccgacaa tctctaccgg tacggtaccg agatcttcac
    78061 caatcaccat acagcgatga cgtttacttt cgagtgccag aatcgagagc agatcatcca
    78121 ccggatagtg aacatacgcg ccctgatctg ccgtctcgcc atacggtatc caccacaaac
    78181 gcagcatcga catcacatgg tcaattcgta atgcgccgca gttttgcata ttggcacgca
    78241 acagctcgat aaacggttca taggcacgcg cggtgatgat atgcgggtcc attggcggta
    78301 atccccagtt ctgccccaac gggccgagga tatccggcgg cgcgccaacc gatgctttca
    78361 ggcaatatag ttcacggtca caccaggttt ccgccccacc ttccgctacg ccaaccgcca
    78421 gatcacgata caagccaatc ggcatttcat agccctggct tatctcccag caggcggcaa
    78481 actggctgta agccagccac tgcaaccaga gataaaaatc gacgtcatca cgatgttctt
    78541 cgcagaactg acgcacttct ggtgaatcca cgttcttata catctctggc catgcaggcc
    78601 agccccagcg catttcgtcc tctttcactt gctgggcatg tagcgcatca aaggctgcct
    78661 gccagaacag gctgtcgccc tgctctgcaa caaactggcg aaacgcggcc atctgctcat
    78721 catcacgttg cgcgaaacct ttccacgcca ttcgtaatgc tgtcattttt agggcggtaa
    78781 ccgtggagta atcgacccag tcggcatcgc gcgcctgttg cagcgtctgt tgcgtggtcg
    78841 gcaactgcca ccaggcctga gcctcttcgc taagatggaa atcttcaacg gcgttaacgt
    78901 cgatataaat cacattcagc caacggcgag aagacgggct gtatgggctg gcgctctccg
    78961 gatttgccgg atagagcgca tgaatcgggt tcaggccaat gaacgacccg ccacgttttg
    79021 ccacatccac cagcatcgct ttgagatcgc caaaatcccc aataccccag tttttttccg
    79081 atcgcagcgt ataaagctga acgcaggcac cccacagctt ttgtttattc agcaaagcct
    79141 gcggttcgta acaacgtttc ggggcgacaa tcacccggca atgcgcgcgc tggtcgtcct
    79201 gggtgagtgt tagcgtgtga taaccttccg gcagcttcgt cggtagattg aacgctttgc
    79261 cccccgttac atggcctttg tactgcgttc cttcttcggt ggtcagctgc cagctatatt
    79321 cgccgctgcc ctccaccacc atcggcattt ttttgccgct ggtataaacc atgacattcg
    79381 ggactggcgt taccgccact ttcgtggcgg tacgttgatg catcgcgtca agcaaacgcc
    79441 gtttggtttc ggcgctaatc gactgcggtt taccgtgggc attgatgtaa ttggggctaa
    79501 tccccgccgc cagcgcggca ttatccagac gtttgctttc catcgagctt ccttagcgtt
    79561 ttgcctgcca gatacgagcc tgataatcgc gaatagagcg atccgagcta aacataccgc
    79621 agcgggcggt attgaggatc gccgcgcgag tccaggcctc ctggtcgcgg tacagcacat
    79681 ccacctgctt ttgtgcctca acataggctg cgaaatccgc catcaccaga tacggatcgc
    79741 cgccctgttt gccgatactg tgcagcatct ggtcgaaggc atgcttatcg ccgtcgctgt
    79801 atttaccgct ttccagctct ttcaatactg cgtccagcac cttatctttc ttccgccatt
    79861 tcaccgggtc gtagcctttg gccagaatag ccttcacttg ttccacggta tgaccaaaaa
    79921 taaagatatt ttcttcaccg actttctcgg cgatttcaac gttcgcacca tccagcgtac
    79981 cgacagtaag cgcaccattg agcgccagtt tcatattgcc ggtaccggaa gcttctttac
    80041 ctgcagtcga aatttgttcg gagatatccg ccgccgggat cagtttttcc gccgccgaaa
    80101 cgcaataatc cggcaggaac accaccttca acttatcgcc aaccagtgga tcgttgttga
    80161 tcacgtcagc cactttgttg atcgcaaaga taatattctt cgccaggtag tagcccggtg
    80221 ccgctttcgc gccgaagagg aagacgcgcg gtacgcgatc agcctgcagg ttttcacgaa
    80281 tttctttgta caacgccaga atatgcagca gattcaggtg ctggcgtttg tactcatgca
    80341 gacgtttgat ctgaatatcg aaaatcgcct gtggattgat ctcaataccg gtacgaactt
    80401 tcacaaactc cgccagacgg actttattcg cctgcttgat ctcgcgatat tgctgacgga
    80461 atttcgcatc atcagcgaat ttttccagat tgatcagctg atcgagatcg ttagcccact
    80521 ctttttgcag tgatttatcc aacagagccg ccagtgcagg gttgcactgt ttgatccagc
    80581 gacgtggggt aataccgttg gtgacgttat ggaatttgtt cggccatagc tggtgatatt
    80641 ccgggaacag atctttcacc accagatccg agtgcagcgc cgcaacaccg ttcaccgcga
    80701 aaccgccaac cacacacagg ttcgccatat gcacttgttt atcgtgcacc accgccagtt
    80761 tggcccacac tttttcatcg cccggccagg ttttctccac cagcgtttta aagcgagtat
    80821 taatttcgtt aataatctgc atatggcgcg gcagtaagcc tttcaccagt ttcacatccc
    80881 agcgttccag cgcttctggc atcagggtat ggttggtgta agcgaaagtt ttgctggtga
    80941 tggcccaggc gtcatcccag ctcatctggt gctcatcggt cagcacgcgc agcagttctg
    81001 gaatcgcgat agttggatgg gtatcgttca gctgaataac ttcgtaatcc gccagttcgt
    81061 gcagtttacg ccccgccaga tgatggcgac gcaaaatatc cgctaccgaa caggcacact
    81121 ggaagtattg ctgcatcagg cgcagctttt taccggcagt atggttgtcg tttggataga
    81181 gaactttggt cagtttttcc gcattgatgc cttgctgttc ggcgcgcagg aaatcaccgt
    81241 cgttaaattt agttagatca aacggatgcg catgcgtcgc ctgccacaga cgcagcggct
    81301 gcgccacgcc attacgatag ccgacaacgg ggagatccca cgcttgaccg gtaatggtaa
    81361 actccggctc ccagcgtccg tctttcatca ctttaccgcc aatccctacc tgcacatcca
    81421 gtgcttcgtt gtggcggaac cacgggtagt tactgcgatg ccagtcatcc ggcgcttcaa
    81481 cctgtttgcc atcgacaaaa gactggcgga acaaaccata ttgatagttc agaccgtaac
    81541 ccgtcgcaga ctgaccgaca gttgccattg agtcgaggaa gcacgccgcc agacgtccca
    81601 gaccaccgtt acccagcgcc gggtcgatct cttcttccag caggtcagtc aggttgatgt
    81661 cataagcctt caacgaatcc tgtacatcct gataccagcc gagattcaac aggttgttgc
    81721 ccgtcagacg accaatcaaa aactccattg agatgtagtt aacatgtcgc tgattcgcca
    81781 ccggtttggc gaatggctga gcacgcagca tttcggccag tgcttcactc actgccagcc
    81841 accactggcg aggagtcatt tcagccgcag aatttaagcc ataacgctgc cactgacgtg
    81901 aaagcgcttc ctgaaattgc ttatcgttaa aaataggttg tgacatagga gttccacttt
    81961 tcttagattt tcaacacaac gttatcgcta gtttgccagg ctcgatgttg accttcctca
    82021 tcctgcgggg gattaggcag ggaggagttg cggggatgag caaggaaatg tgatctcgac
    82081 cacttaaagc tagtgcaaac cacaggatta gcagcaaatc aatgccatac agcgcagaaa
    82141 atctgtatct aagtgcaaaa aatggccgtt gcgtattttc aaaaagcgga aggtaactct
    82201 ataaattaag taaaggagtg aaacagtttc acaagtaaaa tatccagtgt gctccatctc
    82261 attcttaata gatttattaa gatcatcttt ttagatggca ctttcatcag gaatgaagaa
    82321 gaaacccttg cttaaa
//
LOCUS       DAJVQH010000005        68193 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJVQH010000005 DAJVQH010000000
VERSION     DAJVQH010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.005"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..245
                     /locus_tag="OQM87_000419"
     CDS             <1..245
                     /locus_tag="OQM87_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1506686.1"
                     /translation="TIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSIE
                     QRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(292..1050)
                     /gene="deoR"
                     /locus_tag="OQM87_000420"
     CDS             complement(292..1050)
                     /gene="deoR"
                     /locus_tag="OQM87_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCS1506687.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWLIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1108..1704)
                     /gene="ybjG"
                     /locus_tag="OQM87_000421"
     CDS             complement(1108..1704)
                     /gene="ybjG"
                     /locus_tag="OQM87_000421"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1506688.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1989..3215
                     /gene="mdfA"
                     /locus_tag="OQM87_000422"
     CDS             1989..3215
                     /gene="mdfA"
                     /locus_tag="OQM87_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCS1506689.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3256..3541)
                     /locus_tag="OQM87_000423"
                     /pseudo
     CDS             complement(3256..3541)
                     /locus_tag="OQM87_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="OQM87_000424"
     CDS             complement(3627..4442)
                     /gene="ybjI"
                     /locus_tag="OQM87_000424"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCS1506690.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4442..5650)
                     /locus_tag="OQM87_000425"
     CDS             complement(4442..5650)
                     /locus_tag="OQM87_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1506691.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5734..6270
                     /gene="rcdA"
                     /locus_tag="OQM87_000426"
     CDS             5734..6270
                     /gene="rcdA"
                     /locus_tag="OQM87_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCS1506692.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6445..8130)
                     /locus_tag="OQM87_000427"
     CDS             complement(6445..8130)
                     /locus_tag="OQM87_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCS1506693.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            8400..8777
                     /locus_tag="OQM87_000428"
     CDS             8400..8777
                     /locus_tag="OQM87_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCS1506694.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="OQM87_000429"
     CDS             complement(8807..9064)
                     /gene="grxA"
                     /locus_tag="OQM87_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCS1506695.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9224..9511
                     /locus_tag="OQM87_000430"
     CDS             9224..9511
                     /locus_tag="OQM87_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCS1506696.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9495..10218
                     /gene="nfsA"
                     /locus_tag="OQM87_000431"
                     /pseudo
     CDS             9495..10218
                     /gene="nfsA"
                     /locus_tag="OQM87_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="OQM87_000432"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="OQM87_000432"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCS1506697.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="OQM87_000433"
     CDS             11269..11745
                     /locus_tag="OQM87_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCS1506698.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="OQM87_000434"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="OQM87_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCS1506699.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14380
                     /gene="potG"
                     /locus_tag="OQM87_000435"
     CDS             13304..14380
                     /gene="potG"
                     /locus_tag="OQM87_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCS1506700.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="OQM87_000436"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="OQM87_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCS1506701.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="OQM87_000437"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="OQM87_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCS1506702.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="OQM87_000438"
     CDS             16302..16790
                     /locus_tag="OQM87_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCS1506703.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="OQM87_000439"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="OQM87_000439"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCS1506704.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OQM87_000440"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OQM87_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCS1506705.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OQM87_000441"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OQM87_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCS1506706.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OQM87_000442"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OQM87_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCS1506707.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OQM87_000443"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OQM87_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1506708.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OQM87_000444"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OQM87_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCS1506709.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="OQM87_000445"
     CDS             complement(22242..22757)
                     /locus_tag="OQM87_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1506710.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="OQM87_000446"
     CDS             23406..25175
                     /locus_tag="OQM87_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1506711.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="OQM87_000447"
     CDS             25386..25709
                     /locus_tag="OQM87_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCS1506712.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="OQM87_000448"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="OQM87_000448"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCS1506713.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="OQM87_000449"
     CDS             complement(26533..27546)
                     /locus_tag="OQM87_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1506714.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVLTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="OQM87_000450"
     CDS             complement(27645..29075)
                     /locus_tag="OQM87_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCS1506715.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANKHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OQM87_000451"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OQM87_000451"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCS1506716.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="OQM87_000452"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="OQM87_000452"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCS1506717.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="OQM87_000453"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="OQM87_000453"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCS1506718.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="OQM87_000454"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="OQM87_000454"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCS1506719.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="OQM87_000455"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="OQM87_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCS1506720.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="OQM87_000456"
     CDS             complement(36107..36802)
                     /gene="aqpZ"
                     /locus_tag="OQM87_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCS1506721.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     VSGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            37227..38885
                     /locus_tag="OQM87_000457"
     CDS             37227..38885
                     /locus_tag="OQM87_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCS1506722.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38882..39838)
                     /locus_tag="OQM87_000458"
     CDS             complement(38882..39838)
                     /locus_tag="OQM87_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCS1506723.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            39989..41104
                     /gene="macA"
                     /locus_tag="OQM87_000459"
     CDS             39989..41104
                     /gene="macA"
                     /locus_tag="OQM87_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCS1506724.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            41101..43047
                     /gene="macB"
                     /locus_tag="OQM87_000460"
     CDS             41101..43047
                     /gene="macB"
                     /locus_tag="OQM87_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCS1506725.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="OQM87_000461"
     CDS             complement(43120..43344)
                     /gene="cspD"
                     /locus_tag="OQM87_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCS1506726.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43667..43987
                     /gene="clpS"
                     /locus_tag="OQM87_000462"
     CDS             43667..43987
                     /gene="clpS"
                     /locus_tag="OQM87_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCS1506727.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            44018..46294
                     /gene="clpA"
                     /locus_tag="OQM87_000463"
     CDS             44018..46294
                     /gene="clpA"
                     /locus_tag="OQM87_000463"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCS1506728.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46697..46784)
                     /locus_tag="OQM87_000464"
     tRNA            complement(46697..46784)
                     /locus_tag="OQM87_000464"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46748..46750),aa:Ser,seq:gga)
     gene            complement(47038..47256)
                     /gene="infA"
                     /locus_tag="OQM87_000465"
     CDS             complement(47038..47256)
                     /gene="infA"
                     /locus_tag="OQM87_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCS1506729.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47541..48245)
                     /gene="aat"
                     /locus_tag="OQM87_000466"
     CDS             complement(47541..48245)
                     /gene="aat"
                     /locus_tag="OQM87_000466"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCS1506730.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48287..50008)
                     /gene="cydC"
                     /locus_tag="OQM87_000467"
     CDS             complement(48287..50008)
                     /gene="cydC"
                     /locus_tag="OQM87_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCS1506731.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTQLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(50009..51775)
                     /gene="cydD"
                     /locus_tag="OQM87_000468"
     CDS             complement(50009..51775)
                     /gene="cydD"
                     /locus_tag="OQM87_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCS1506732.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51898..52863)
                     /gene="trxB"
                     /locus_tag="OQM87_000469"
     CDS             complement(51898..52863)
                     /gene="trxB"
                     /locus_tag="OQM87_000469"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCS1506733.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53408..53902
                     /gene="lrp"
                     /locus_tag="OQM87_000470"
     CDS             53408..53902
                     /gene="lrp"
                     /locus_tag="OQM87_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCS1506734.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            54037..58143
                     /gene="ftsK"
                     /locus_tag="OQM87_000471"
     CDS             54037..58143
                     /gene="ftsK"
                     /locus_tag="OQM87_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCS1506735.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            58298..58909
                     /gene="lolA"
                     /locus_tag="OQM87_000472"
     CDS             58298..58909
                     /gene="lolA"
                     /locus_tag="OQM87_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCS1506736.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58920..60263
                     /gene="rarA"
                     /locus_tag="OQM87_000473"
                     /gene_synonym="mgsA"
     CDS             58920..60263
                     /gene="rarA"
                     /locus_tag="OQM87_000473"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCS1506737.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            60354..61646
                     /gene="serS"
                     /locus_tag="OQM87_000474"
     CDS             60354..61646
                     /gene="serS"
                     /locus_tag="OQM87_000474"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCS1506738.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61886..64330
                     /gene="dmsA"
                     /locus_tag="OQM87_000475"
                     /pseudo
     CDS             61886..64330
                     /gene="dmsA"
                     /locus_tag="OQM87_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            64341..64958
                     /gene="dmsB"
                     /locus_tag="OQM87_000476"
     CDS             64341..64958
                     /gene="dmsB"
                     /locus_tag="OQM87_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1506739.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     HYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            64960..65823
                     /gene="dmsC"
                     /locus_tag="OQM87_000477"
     CDS             64960..65823
                     /gene="dmsC"
                     /locus_tag="OQM87_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCS1506740.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(65859..66485)
                     /locus_tag="OQM87_000478"
     CDS             complement(65859..66485)
                     /locus_tag="OQM87_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1506741.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            66800..67948
                     /locus_tag="OQM87_000479"
     CDS             66800..67948
                     /locus_tag="OQM87_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1506742.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT        16945 a        16861 c        17856 g        16531 t
ORIGIN      
        1 tgaccatacc gcgtgggcag ctgaaaaacc tgaaggcgag ctacacgtta acggaaccgc
       61 agcttaccgc accgctgaaa aaaggtcagg ttgtcgggac cattgatttc cagcttaacg
      121 gtaaatccat tgagcagcgt ccgctgatcg tgatggaaaa tgtggaagag ggcggattct
      181 ttggtaggat gtgggatttc gtgatgatga aattccatca gtggttcggc agctggttct
      241 cttaatcttc tgataaccgg atggcgcgaa acgtcatccg gttatacgtc attaatacat
      301 caacttaata cggtgcgtct gcgcgtactt cacatactca tcttccgggc aacaatcact
      361 caccacaata tcaaagcgtt tcaggtcacc catgcgcgcc ggacgcacct tgccaaattt
      421 actgtggtcg acaaccagca catgcttttg cgccatcgac atggcccagt gttttaccgg
      481 caactcttca agattaaaac aggtagcgcc tttactgaca tgcacgcccg ccgcggaata
      541 aaaagcgata tccgggcaaa aattattcaa cgtttgctga aaatcgatgg gtttgaaaat
      601 ggcgttgctg gcgtgaaatt caccaccgca cagaaatgcg cggcaatggg gtttctcttt
      661 cagcgccaga aaggtattta gcgaataaca aacggcggta aaagggattt cattatcaat
      721 cgcttcaata agccacggcg tggtggtgcc acagtcaaaa aagagggtct gatcgggttc
      781 taccagtgtc gcagccagtt ttgccgcccg gcgtttttct tccaccaggc gggatttttg
      841 atcgcttaac aggtaatggc tggcactgcg cggttccaga acaatatagc cgccgagcaa
      901 aacgacgggc gcactgtggt tgttcagatc gcgacgaatc gtcatctccg aaaccccaag
      961 cagggcggcg gcgtctttaa gatgtaactt atcgctgcgt tttaattctt gcagcagctg
     1021 cccgatacgc tcttcgcgac gtgtttccat aatccctctg aatagttatt gaagcgagcc
     1081 gctcaatact acacttttta gcagagatca gtcacgcacc cagcctttgc ggatcggtaa
     1141 tgcaaaacag acgcgatacc acgattgcag acgttgatag agtttatgcc ccatcgcttg
     1201 ccagataatc tgggcactaa ggcagccaat catacctgcc agcaatccac cgagcatatc
     1261 cagcggccag tggacgccaa gataaacgcg cgaccaggca atgacgacgg ccagcaccat
     1321 taaaagtgag ccggaccaca ggcgatgcca gcataaaaat gccagtgcaa aggtgaaaat
     1381 caccgtaccg tgatcgctcg ggaatgagtc atccgccgca tgatgcagga agttatagcc
     1441 gatattttcg acaaagggtc ggtcgtgcgg aaaaagatgt cccatcgtcc aggacacaaa
     1501 caggctgacc gccagcgcga tagcgatttt tatcaccagt tgccgttgtg ctgtaagccc
     1561 ccacaaccaa agtaccgcgg ccagcaacgg caccacggta atcaaatctt tagcaataaa
     1621 aatcgccaac gagatcatcc acggagccga gtctggcgtc gcgttaataa gagagaatag
     1681 agagagattc aaattttcca gcataacttc ccgacgcaaa gtgattaaaa gggagccaat
     1741 acaggcaagt cgttgagaat aaagtgcagg ttaaactggg taaagcggca tcgtcttatt
     1801 tccctcaagc ggcctgttta cggtgggtga ttgtaacggg cataggttaa ataaaactta
     1861 aagaaagcgt agctatactc gcaataatgt aagaatgtgc ttaaccgtgg tttcagctac
     1921 aaaattcgct ttctcgttag ctgcgctttt attaaactct gcgcgattat tattggcgaa
     1981 gaaattgcat gcaaaataaa ttagcttccg gtgccaggct tggacgtcag gcgttacttt
     2041 tccctctctg tctggtgctt tacgaatttt caacctatat cggcaacgat atgatccaac
     2101 ccggtatgtt ggccgtggtg gaacaatatc aggcgggcat tgattgggtt cctacttcga
     2161 tgaccgcgta tctggcgggc gggatgtttt tacaatggct cctggggccg ctgtcggatc
     2221 gtattggtcg ccgtccggtg atgctggcgg gtgtggtgtg gtttatcgtc acctgtctgg
     2281 caatattact ggcgcaaaac attgaacaat tcaccatgtt gcgcttcttg cagggcataa
     2341 gcctctgttt cattggcgct gtgggatacg ccgcaattca ggaatccttc gaagaggcgg
     2401 tttgtatcaa gatcaccgcg ctgatggtga acgtggcgct gattgctccg ctacttggtc
     2461 cgctggtggg cgcggcgtgg atccatgtgc tgccttggga ggggatgttt gttttgtttg
     2521 ccgcattggc agcgatctcc tttttcggtc tgcaacgagc tatgcctgaa accgccacgc
     2581 gtataggcga gaaactgtca ctgaaagaac tcggtcgtga ctataagctg gtgctgaaga
     2641 acggccgctt tgtggcgggg gcgctgggat tcgttagcct gccattgctg gcgtggatcg
     2701 cccagtcgcc gattatcatc attaccgggg agcagttgag cagctatgaa tatggcttgc
     2761 tgcaagtgcc tattttcggg gcgttaattg cgggtaactt gctgttagcg cgtctgacct
     2821 cgcgccgcac cgtacgttcg ctgattatta tgggcggctg gccgattatg attggtctgt
     2881 tggtcgctgc tgcggcaacg gttatctcat cgcacgcgta tttatggatg accgccgggt
     2941 taagtattta tgctttcggt attggtctgg cgaatgcggg actggtgcga ttaaccctgt
     3001 ttgccagcga tatgagtaaa ggtacggttt ctgcggcgat gggaatgctg caaatgctga
     3061 tctttaccgt cggtattgaa atcagcaaac atgcctggct gaacgggggc aacggactgt
     3121 ttaatctctt caaccttgtc aacggaattt tgtggctgtc gctgatggtt atctttttaa
     3181 aagataaaca gatgggaaat tctcacgaag ggtaaaaaaa tgcctgattg atttgtgcga
     3241 tcaggcattc tcgaattaat ggtgatggtc gtcaatctgg tgttcgataa ccatcccttc
     3301 acctacgctg gcaagatggc gaacataagg atgcgggcgg taagccggag ctggcacagg
     3361 agccacatag acggtttgcg gcacagtcgc gacgctgaca cgcttgtgga acgctgactg
     3421 aatcaggaac cacaaccact ttgtatccac tcggcacatc gacggtgaca ctttgcgcca
     3481 tcgccacacc agtaaagccc attaaaagcg cgcccaacag tagacaattt ttcataatca
     3541 tttcgcctga aataatgcag cgggttaatt tccgcgaatt atgcagcctt cattcaggca
     3601 ttgtgttgcc gggcgtgctg ccgtttttat tggtcaaatg gcgcttcgtg tttaagaact
     3661 ttatcgatca catccagtac gccttcacgg ttattggagc ctgcccggta ttttgccgct
     3721 gcgacgaccg cgctgccggc atttttcatt gcaaaactaa agcctgcctg acgcagcatc
     3781 tcaatatcgt taccgccatc gccaaagacc accacttcgc tgtcgtctat tccccataat
     3841 ttctgcagtt ggcgaaggcc attggcttta tgtacgccgg ggataatcag atcgatgctg
     3901 ccgttgccgg tgtggactgg caccataata tcgccgatgg cctcatgtaa tgctttttgt
     3961 acttgtggaa tcagttcatc ggaaagattc aggccaaact taaagaaaat atcttctaag
     4021 ttgtcaaagt tatcgacgta ttccagacgg tgataataca tttccgccac cgttttcatg
     4081 gcatcgtcat attttttgag tgtataggcg ctattttttc cgcaggcaat aatttccact
     4141 tccgggcgcg tcagcaaatg ttccacgaca gtagcaaacg catccttcga cagctcgcca
     4201 ttaaaaacat ctttgccttc actcactacc cagccgccgt tttcagccac aaaggcaatt
     4261 tcattcgcaa tttcagggaa gaaagagatc aactgataat attgattccc gctggcgacc
     4321 acaaagcgaa tgccttgtgc tttcatttgc tgatactgag ccataaaccg ctcacggtta
     4381 taggtttttt gatcgcttaa gaaagtacca tccatgtcta ccgcaattaa tttaatactc
     4441 atcaactatt ctccatcgcc gtctgcgttt tggtatcggg tttggcgacg gctttcgcga
     4501 caatagccgc gagaataacc agcgccagta caaccagcat tgcactacgt aatccataat
     4561 gttcgccgag atagcccagc agcggcggcc cgacgaggaa agccagataa ccggtcgttg
     4621 ctaccacact gacgcgggtt ggtgcatcgg ggccggtatc gctggcggca gaaatggtca
     4681 gcgggaagcc cagcgaggca cccagtcccc aaagtacaac agacactcct gcgacccagg
     4741 cgctatcgac aaaaataatc agcccaatac ccaacgcccc cattagtgca ctggcccgaa
     4801 ccacggcaac gcgactgtaa cggtcgatga accaaccgcc ggtgaagcgt ccaacggtca
     4861 tccccagggt aaaaccggca taaatcagtg agccggaagt ggggctaaaa ccgtgaccat
     4921 caaccattaa taagggtaac cagtcgttgg cagaaccttc ggcaaaggcc atcgccagca
     4981 ctacaacacc aatcaacagc aactggatat cgcgataaaa aggaatgcct ttttcgccat
     5041 gctgggtgcc atcggcagca tttttgcccg taccgtcagg gattgcctga atggcgatat
     5101 aaataggcgc gatacctacc agcgccgcca ataaaatgtg caccgttgcc ggaacgccaa
     5161 aggccgtcag tgccatcccg acaccagcgc ctgccagcgt gcccaggcta taaaaaccgt
     5221 gcatcatcgg caaaaccgtt ttattcattt ctcgctcaac ggcggcacct tcaacgttta
     5281 tcgccacttc cgcagaacca aaacttgccc caaagacgcc gagaccaacg gcaaagagca
     5341 ggggcgatgt cagccagagt gccagactta atatcatcat cccgatcaat gcgcaggaca
     5401 tcgtgaccag gatgacatta cgtgtcccaa agcgtttcac taaccacgcc gagcagagaa
     5461 taccgctcat cgaaccgatc gacagaccaa agagaacacc gcccatttca gcgatcgaga
     5521 cagagagaat atcgcggata gcaggcgtac gggttgccca ggacgccatt aacaggcctg
     5581 gcaaaaagaa gaacataaac agcgccctgg ttcggcgttt caatgcatta cgtgaagaat
     5641 ttacggtcat agatcacgtc aaaataagaa gagggaagac aacattagca aggttgtgta
     5701 catttgtaca caattgcaga cagaggaaat gacatgcgtc gcgctaacga tccgcaacgg
     5761 cgagaaaaaa ttatccaggc cacgctggag gcggtgaaac tttacggaat acatgctgtt
     5821 acgcaccgca aaattgctac ccttgccggg gtaccgttgg gatcgatgac ctactatttt
     5881 tcaggaattg atgagttgtt gctggaagcg ttcagcagtt ttactgagat tatgtcccgg
     5941 caatatcagg cattttttag cgatgttagt gatgctcaag gcgcatgcca ggctatcacc
     6001 gatatgatct acagctcaca ggttgcaacg ccggataata tggagctgat gtaccagctc
     6061 tacgcgctgg ctagccgaaa accgctatta aaaacggtaa tgcaaaactg gatgcagcgc
     6121 agtcagcaaa cgctcgaaca atggtttgaa cccggaaccg cccgcgcgct tgatgcgttt
     6181 attgagggga tgacgctgca ttttgtcacc gaccgtaagc cgctatcgcg cgaggagatt
     6241 ttgaggatgg ttgagagggt tgcagggtag tagataaatt ttagataaca aaaaacccat
     6301 caaccttgaa ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa
     6361 gcagtggcac taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat
     6421 ttcattttca gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt
     6481 ccctgccagc gtcagcagga cgttggtgat tgcgtaggtg cccgcatagc ccagcgcagg
     6541 gatgttactg cgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc
     6601 catcattgcg ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa
     6661 acagataacc acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat
     6721 cgcgcccagg ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac
     6781 catcaagccg aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg
     6841 gtggttagca cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc
     6901 catgccgaaa ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac
     6961 aaagaaggcg caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat
     7021 gcgatcggcg atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt
     7081 gttaagcacg acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag
     7141 gaagcaaccg tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt
     7201 tttaacgacc acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc
     7261 tttaccgtta cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc
     7321 tatttcatcg cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg
     7381 tcgaatacgt tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt
     7441 gccgtcggtc caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata
     7501 aaccttacgg ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt
     7561 ctgtaagtcc tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag
     7621 accgattaaa taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg
     7681 cctgctttcc atgccggaat gatgcagtgt atcgccagca ccgaccagaa ccggtgtcga
     7741 cgtcatagag cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc
     7801 taaccctaag gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt
     7861 tttcccatcg cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa
     7921 aataaacagc ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc
     7981 taataacagc gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg
     8041 taactttccg agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc
     8101 atttaacaat tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct
     8161 gttgaaagaa atggtaattt acgataatgt gttttaccag aattcagggt gcagattcat
     8221 tcagcgcacc taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt
     8281 atcaacggct atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc
     8341 ttactgaacg ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag
     8401 tgaaacataa acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt
     8461 gcctttttct ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg
     8521 gcttgctgtt tttcattctt cctggagcag ttgccagttt tttttcacag cgtagagaag
     8581 tcctgaaacc tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc
     8641 tgtttttttc accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt
     8701 tctggtgtgc gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc
     8761 acagaaaaaa tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat
     8821 tttctttcac ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa
     8881 tctgcggcac ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc
     8941 cttccgcacg aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct
     9001 cagccagatc ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt
     9061 gcattatttc tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg
     9121 aaagtaggtt taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag
     9181 tgaaaagttt tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa
     9241 attgcctaaa ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct
     9301 gctgtcggta agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat
     9361 tctgatgatt tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca
     9421 ggtggtaaaa cgtcttgccc cacagctgat gaaccgtcca ccgcaatatt cacgttcaga
     9481 aagagaaaaa gataatgacg ccaaccattg aacttatttg tggccatcgc tccattcgcc
     9541 atttcactga tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg
     9601 cgacgtccag ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat
     9661 tacgtgaaga actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt
     9721 tctgggtgtt ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg
     9781 gcctggcgga acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat
     9841 taaccgcagc ggaatcggct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg gcctggggat gagttttttt
    11341 gaatgtaata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 cttgaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggtgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gcgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctag ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagcagtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcac agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcctggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgctgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggcaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca ccaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag ttagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaatc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg aggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacgtg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tatgaataca aaaggtgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tggatgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatgctttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata atcgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcca gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gtgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat cgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc agaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgtttgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccacct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gcaggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgccagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gtgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgtgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcgt cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaaca caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 acgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaactaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 tttcctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggt taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg
    35761 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
    35821 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
    35881 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
    35941 caggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
    36001 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
    36061 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
    36121 ccagcagagt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    36181 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    36241 ccgggttaac agaagtgtta gtcaccggaa tactgattaa gtgaatcagg gttaaggcca
    36301 gaccaatagc gatcggcgca aaacctgccg gagcgaattt gtcggttgca ccgtggatca
    36361 ctaacaggaa gcccgcactc aataccagtt caactaccag cgcggaaagc atggaataac
    36421 cgcctggtga atgctcgcca taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac
    36481 ccgttttacc actgacaatt aaatacagca gcgccgctgc aacaataccg ccgacaacct
    36541 gggcaatcac gtagccaacg acttcttttg ccggaaaacg tccgccagcc cataaaccaa
    36601 tagtgaccgc cgggttaaaa tgaccaccag aaatatgacc aacagcgaag gccatcgtca
    36661 gaacggtcaa accaaatgcc agcgccacgc cggcaaaacc aatgcctaat tccgggaagc
    36721 ctgcggccag tacagcacta ccacagccac caaaaacaag ccagaaagta ccaaaacatt
    36781 cagctgctaa ttttctgaac atatccacct caattaaaaa ttgaccctgt gaaaaatagg
    36841 gtcgttttat agggtcgtcg taaaaaatga cgacggaaat aatgcgcggc tattttaaaa
    36901 acgaaggcga gtcattcacc agataaataa atccagtaaa tttgatttag ggcaacagcg
    36961 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcaagcaaaa gatttaatcc
    37021 tttaggcgta ataaaaaata atttatcatg ctaattattt gattttgttg tttttgcggg
    37081 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca attgccatgc
    37141 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
    37201 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
    37261 gcggtatcaa ccgtttgtcg ttgatgctcg aacaaaacaa cgtcctgatt ggggagaacg
    37321 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
    37381 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
    37441 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
    37501 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
    37561 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
    37621 ataaagacgg acatccgatt gatgttgagg atattaacga tcaggcacgc catctggtgc
    37681 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
    37741 cggtgccaaa tgtccccaat gtggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
    37801 agttgtcctc acatccgcaa aatctctctg atggacagat tcgtcaggga ctttccgcaa
    37861 tggtgcagtt gcttgagcat tatttctctg agcagggggc tggacaggcg cgatatcgtt
    37921 taatgcggcg gcgggccagc aatgagcaac gaagctggcg ctatctggac atcatcaacc
    37981 ggatgattga ccgacctgga gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
    38041 ctttattgca ggcaaaaggc acattgcgac tggataaaga cgcgcgtccg ttattgctaa
    38101 ttgaagatcc agaaacccgc ctgcatccga ttatgctttc cgttgcctgg catctgttga
    38161 atcttttgcc tttacagcgt attgctacca ccaactctgg cgagctgctt tcgttaactc
    38221 cagtggaaca tgtttgccgc ctggtgcgtg aatcttctcg tgttgccgcc tggcgtctgg
    38281 ggccaagtgg cttgagcacc gaagacagcc gccgcatctc ttttcacatt cgttttaatc
    38341 gtccgtcatc gctgtttgct cgctgctggt tgctggtgga aggggaaacg gaaacctggg
    38401 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
    38461 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
    38521 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
    38581 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
    38641 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
    38701 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
    38761 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
    38821 taccaacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
    38881 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
    38941 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
    39001 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgactcc cagaacgcgt
    39061 tgtaatcagc atggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
    39121 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
    39181 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
    39241 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
    39301 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgga
    39361 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
    39421 actgctcacc gtttttgcct tccagtttag ccagttgcag cccgggggta ttcaaatata
    39481 agctgaattc ttccgccgac atacacccgc ggagtaacgc ataatggtaa cgtaacgcct
    39541 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
    39601 gtcgcggctg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
    39661 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
    39721 agcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
    39781 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
    39841 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
    39901 tgtgattact aacgtaaatt ttagggtttt gttgatattt cgttgaagtt aatgacccgg
    39961 attggcatgt ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
    40021 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
    40081 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
    40141 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgta ggcgcgcagg tcagcggtca
    40201 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
    40261 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
    40321 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
    40381 tcagcaacgt ctggcacaaa cgcaagcggt ttcactgcag gatctcgata ccgccgcgac
    40441 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
    40501 ggcctctctc gatacggcta aaaccaatct cgattacacc cgtatcgttg ccccgatggg
    40561 cggggaagtg acgcaaatca ccactctgca aggccagacg gtgattgccg cacagcaagc
    40621 accgaacatt ctgacgctgg cagatatgag cactatgctg gtaaaagcgc aggtttctga
    40681 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtgc ttggcgatcc
    40741 actgacgcgc tacgaggggc aaatcaagga tgtgctaccg acgccggaaa aggtaaacga
    40801 cgctattttc tattacgccc gttttgaagt acccaacccc aatggtttgc tgcggctgga
    40861 tatgaccgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgttga cgatccctct
    40921 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
    40981 tgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
    41041 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
    41101 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg tgatgagcag
    41161 gttgaggtgc tgaagggcat cagcctcaat atttatgcgg gtgagatggt cgcgattgtt
    41221 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
    41281 accagcggca cctatcgcgt agccggtcag gatattgcca cgctggacgc cgatgcgctg
    41341 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
    41401 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ccggtcttga gcggaaacag
    41461 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
    41521 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
    41581 aacggcggtc aggtaattct tgccgatgaa ccgaccggcg cactggacag ccattctggc
    41641 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
    41701 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
    41761 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
    41821 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
    41881 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
    41941 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
    42001 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
    42061 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
    42121 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
    42181 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
    42241 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
    42301 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
    42361 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
    42421 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
    42481 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
    42541 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
    42601 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
    42661 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggcgg tattggtgta
    42721 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
    42781 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
    42841 ctggtcggtg gcgcgttggg aataacactg tcactgttaa ttgctttcac cttgcagctt
    42901 ttcttacccg gctgggagat tggtttttca ccgttggcgc tgctgctggc gtttctctgc
    42961 tcgacggcca ccgggatttt atttggctgg ttacccgcac gaaatgcggc acgactggat
    43021 ccagtagatg ctctggcacg ggagtaattt ttgagataaa aatgccagcc gatcgggctg
    43081 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
    43141 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
    43201 aacggattgt ccagctttta gcgttctgta accatccatc tgaatggtgg aataatgagc
    43261 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaacctttgg cattgttgaa
    43321 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
    43381 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
    43441 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
    43501 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
    43561 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
    43621 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
    43681 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
    43741 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
    43801 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
    43861 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
    43921 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
    43981 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
    44041 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
    44101 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
    44161 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
    44221 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
    44281 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
    44341 tctggtcgct atcttcagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
    44401 agtcagccgt ctcgatgtgg tgaactttat ctctcatggc acgcgtaaag acgagccgac
    44461 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
    44521 tatggagaat ttcacgacga acctgaacca gcttgcgcgt gtgggcggaa tcgacccact
    44581 aattggtcgt gagaaagagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
    44641 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcag aaggtcttgc
    44701 ctggcgaatt gttcagggtg atgtgccgga agtgatggct gactgtacga tttactctct
    44761 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
    44821 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
    44881 caccattatc ggtgcgggtg cagcgtctgg tggtcaggtc gatgcggcta acctaatcaa
    44941 accgttgctc tccagcggta aaattcgcgt aattggttcg acaacctatc aggagttcag
    45001 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
    45061 accgtcgatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
    45121 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
    45181 cattaacgat cgtcatctgc cggataaagc cattgatgtt atcgacgaag cgggcgctcg
    45241 cgcacgtctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
    45301 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
    45361 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggccaggata aagccattga
    45421 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
    45481 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
    45541 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
    45601 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
    45661 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
    45721 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
    45781 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ctaacgccgg
    45841 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
    45901 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
    45961 ctggttcgat catctgtcaa ctgacgtgat ccatcaggtg gtggataaat tcatcgtcga
    46021 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
    46081 ctggctggcc gagaaaggtt acgaccgggc aatgggcgca cgtccgatgg cgcgtgtcat
    46141 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
    46201 cggtcaggtg acggttgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
    46261 tgcacaaaag cacaaggcgg aagcagcgca ttaatctgat tgtcaggtag gttggtgaag
    46321 tccgtaatct cgtcaggggt tacggacttt ttatttatgg ggtggaacgg tgaaaaccct
    46381 atttttggag gtgaaggtaa gttgttgata attagtgctg ctggaaggta aggataaaaa
    46441 agggtgctgc aggagaatgg gatggttttg ctttattaac aacgggctaa acgtgtagta
    46501 tttgagttca ctgccgtaca ggcagcttag aaatcgacgg ggtgcggtaa aacctttgcg
    46561 aacgcgttca ctgccgtaca ggcagcttag aaattcacag gtaacatact ccacccgccc
    46621 accatgttca ctgccgtaca gacagataaa atgcgaaaaa aaagctcgca ctttcgtacg
    46681 agctcttctt taaatatggc ggtgaggggg ggattcgaac ccccgatacg ttgccgtata
    46741 cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg ttttgctacc
    46801 aaacctcatg ggtggcaacg gggcgctact atagggagtt ggagtaaaac ggtcaagaag
    46861 aattttaatg ataattattg tttgctcata ctgtaaacaa cttgtgcagt atatctacat
    46921 cgagacaagt tatggactta tacttccaaa gtacttcata catatcacaa aataaaaagg
    46981 ccggttaaac cgacctttta ctcgttcttt ctcttcgccc atcaggcggt aaaacaatca
    47041 gcgactacgg aagacaatgc ggcctttgct caggtcgtac ggggtcagtt caacagtcac
    47101 tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt ttaccggaga tgtgtgcagt
    47161 aaccacgtga ccgttttcta actctacgcg gaacatggta ttaggcaacg tttcaagaac
    47221 ggtaccttgc atttcaatat tgtcttcttt ggccatctaa tcctctgggg tatcactacc
    47281 gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat aagtaaagat ttgcgcgcta
    47341 aatcgcaaca aacaggttcg gcacattact ccgaaaacac acggctaagc cgcaccaaaa
    47401 gcgcaacgta taagggagcg gtgagataaa cgatgggcgt tacctgacgc gaaaaattcc
    47461 ttatcggcag cggggtaatg agcgtaacca actctgcgac cgcaattata acactctggg
    47521 gagaaatgtg ccgaaaacat tcattcttgt ggtgaaaaca agcaccgtgg tacccagaaa
    47581 ttattcggca atcgtccgag gcgcatttga ttgagataat taaggtaatc ccggcgggga
    47641 atttcgcagg caccaagcga tgctgtgtga tcgttaagga cctggcagtc gataagctta
    47701 ccgccatgac cgataaattc ctcacagaat accagaagcg ccgttttaga cgcattttcc
    47761 atccggctga acatggactc gccacaaaat agcgttccct gggccacgcc gtacataccg
    47821 ccgacaagct catcttcacg ccagacttca atggagtggg catgaccgag ttcgtgaagg
    47881 cgatggtagg cttcgaccac gccacgcgtg atccaggttc cttcttcgcg atcgctggca
    47941 cagccttcaa tgacctgacc aaaagcgtaa ttcatcgtga cacgataggg cgagcgttta
    48001 tgaaatcgct tcatactacg gctgatatgc agtgattctg gccatagcac cgcgcgggga
    48061 tcgggcgacc accagaggat ggggtcgcct ggagaaaacc acggaaaaat accacgctgg
    48121 taagccatta acaggcgcgc agggctaaga tcgcccccaa gtgccagcag gccgttaggc
    48181 tcacgtaatg cgccttccgg ggaagggaag gctattgaat ggcgagaaag ctgaaccagg
    48241 cgcatgaccg caaaactcca cgcaagtcgg atcgttcaat aatagcttac aaaccctgct
    48301 tgaactggta ataacgcccc tgtctggcaa gcagttctgc gtgagtacct tgctcaataa
    48361 tttgcccgtt gtccatcact attatttgtt ggaaacgaga gagtccgcga agtcgatggg
    48421 tgaccattaa caccgttttc tcacgcatca tttttgcaag caattcaagg atctggcttt
    48481 cggttgtggc atctaagcct tcggtaggtt catccagcaa caccagtggc gcatcatgta
    48541 acagcgcacg ggcgatagcc agacggcgca gttcaccacc ggagagctgg cgtccgcctt
    48601 cacctaacca actgttgaga cctgcatcct cgagcagctt ctccagacca acgcgacgca
    48661 agatctccga cagagcctca tcactactgc caggcgaggc gagtaacaga ttatcacgca
    48721 gcgtggcgct aaacagatgc actcgctgag gaacaacgct gatggtctgt cgtagagccg
    48781 cttcattcag gctggctatg gggctatcgt taagcaaaat ctcgccctgt tgcgggtccc
    48841 atgcgcgggt cagcagttgt aacagtgttg atttgccgca tccggttcgc ccgagaatcg
    48901 ctatatgttc cccggcgttt acctgaagag aaatcccttt aagtgcctgt tgggattgct
    48961 ccggataagt gaactgaaca tcccgtaatg tcagcgaaac gcgatcggca acacgagttt
    49021 gggtatcagg gaaggtgacc tccggttttt gatccgttaa gtcagagata cgtacggcag
    49081 aggcaatgac ttgccccaga tgctgaaatg cacccgttac tggtgccagt gcttcaaacg
    49141 cggctaacgc acaaaagaca aacagggcaa ttaatgcgcc gggttgagca ttgccgccaa
    49201 cgccgccaga cgccatccac agcatcagga tcaccgctaa cgcgccaatg agcagcatta
    49261 tcgcttgcga caatgcggtc agttcagatt gacggcgttg cgcttccagc cattgaattt
    49321 ctgtattctc tagttgcgtg cgataacgat cgctggcacc aaaaatggtc agctcagctt
    49381 gcccttgcag ccaggccgtc agttgttggc gatactgtcc gcgaagatga gtcagatttt
    49441 gcccggtgct ttttcccgca cgataaaaca gcggtggcat caggaaaagc gtcagtaaca
    49501 taacgctgcc cagcgtaaag gcgagggtga aatcaaggaa acttaacccg attgtcacca
    49561 ccataatcac cacaaaagcg cccaccagcg gcgagataac gcgcaggtaa agatgatcga
    49621 gcgtatcaac atccgccacc acgcggttga gcaattcgcc ctgacgatag cgcgccagtc
    49681 cggcagggga gaggggcaac aatttgctga aggtgtaaat gcgcagatgc tgcaacacgc
    49741 ggaaagtcgc gtcgtgactt accagacgtt caaaataacg cccggcagta cgggtgattg
    49801 cggcaccacg cacgcccgca gcgggtagca tatagttgaa gctgtacagt ccggcaaccc
    49861 ccgcaaccgc tgaggccgag aggaaccagc cggaaagtgt caacagaccg atactggcga
    49921 gctgcgtcac aattgccagc acaataccaa gacttaacat ccatttatga cgtttataca
    49981 gtgccagata gggtagcaaa gcgcgcattt aaatctcctc ctgacgatgg gccagtaatg
    50041 tggcgaatgg gccaccagcc acacttaatt ccgcgtaacg tccttgctca ataatccggc
    50101 catcctgcat aacccaaatg acatcccagt cagcaagatc ttctaactgg tgggtgacca
    50161 ttaacgttgt ctggcgcaga gaggcggcat tcagcgcctc cattacgcgc tgttcactgt
    50221 gagcatcaag gctggcagcg ggttcatcca acagtaatag cgaacaggga tttagtaacg
    50281 cacgggccac cgccacgcgc tgcgcctgcc ccacggaaag gcgggcagcc tggtcgccaa
    50341 caggtgtatc aacgccttgt ggcaggagcg gtagaaactc gctgacccag gcgttatcca
    50401 gcgctgcttg taattcttgt tcgctggcat caggtcgcgc cagtagtacg ttatcccgca
    50461 attttgctgc cggtaattgt gggttttgcc caacccagga gagatgttta cgccatgatt
    50521 ctggtgataa atcacgtaat tctatcccgt tgattcgtaa tgatccctga tatgagagaa
    50581 aaccagaaag cgcgttcagc agtgagcttt tacctgaacc gctgcggcca accaacactg
    50641 cacgttggcc tgctggcaaa gtaaagttca gcggtccggc cagcgtttta ccttccggcg
    50701 acgtgataaa cagatcctcg gcctcaatgg tcagcggatc ggtcaatgct aattccgcct
    50761 caccgcgctg cggatgggcg agcggggttt ccataaacgt tttcagactg tcagctgcac
    50821 caacagcctg ggctttagca tgataaaacg taccgagatc gcgtaatggc tggaaaaact
    50881 ctggcgcaag gatcagggcc agaaaacctg cagccagcgt caccccagta tcgtagtgac
    50941 caaaatccag ctcgccgaga taggaaaaac caaagtagac cgccaccaga gcaattgaca
    51001 gcgaggtaaa aaattcgaga atgccggagg ataaaaacgc cagtcgtagc acttccattg
    51061 tccgttggcg gaaatcttcc gaagcagaac gaatactttc aatttcagct tcaccacgac
    51121 caaaaatacg caatgtttcc atgccgcgca ggcgatcgag gaaatgccca cttaagcgag
    51181 caagagcgag aaagttacgc cggttagcat cggcagcccc cattccaacc agcgccataa
    51241 acaacggaat tagcggtgct gtgcccagca gaatgagcgc cgcagcccag ttagagggga
    51301 agatcgccac cacaatcagc aacggcaccg acactgccag cgccatttgc ggcagatagc
    51361 gtgcatagta atcatgcata tcgtcaattt gctcgagtac cagcgtcgcc cagctccccg
    51421 caggtttacc ctgaatccac gctggccctg cttgttgcag acggtcgaga acctgacggc
    51481 ggatggcaaa gcggatatgc tgcccggcgt gataacccac ccgttcgcgt aaccagacca
    51541 cccatgcgcg caatacaaag gtcagaacca gtaacgtaaa gggaagcagc agggcttcac
    51601 ggggaatatt ctccataatc atatgttgca gaattcgcgc catgaaccag gcctgggcaa
    51661 tgatcaatat gccgcttaca aagcccagca gacgagaaat attcagccaa cgttgggaga
    51721 tgacgctttg ctgttttaac cagcgggtta actctttttg acgagattta ttcattgcac
    51781 gcttagcagg tgagttatca gaattatttg cagagcaatg ttacaacggg gaaaaaataa
    51841 aggcgaccca tagtcgcaag gtgtcgcctt ctttactttt gttactgatt tgtaaaatta
    51901 ttttgcgtca gctaaaccat cgaggtagcg ttccgcatca agtgctgcca tgcagccagt
    51961 accggccgaa gtaatggcct ggcgataaat gtgatccatc acgtcgcctg cggcaaagac
    52021 gccaggaatg ctggtctggg tggcattacc atgaataccc gactgtactt tgatgtagcc
    52081 gttttccagt tccagctgcc cttcgaaaat cgcagtattc gggctgtgac cgatagcaac
    52141 aaacagaccg gcaacgtcga gtgactcgat gttatcgctg ttttgcgtat cgcgcagacg
    52201 aacgccagtg acacccattt gatcgccggt cacttcttcc agcgtacggt tggtgtgcag
    52261 aatgatgttg ccgttctcca ctttatccat cagacgctta ataaggattt tttccgcgcg
    52321 gaaaccgtca cggcggtgaa tcagatgcac ttccgaagcg atgttagaca gatacagcgc
    52381 ctcttcaacc gcggtattgc cgccgccgat gaccgcaact ttctggttgc gatagaagaa
    52441 accgtcgcag gttgcacaag cagaaacccc acggccttta aaggcttctt cagagggcag
    52501 gccgagatag cgtgcagaag ctccggtggc aataatcagc gcgtcgcaag tgtattcgcc
    52561 gttatcgcca ttcagacgga acggacggtt ttgcagatcc accttgttga tatgatcaaa
    52621 aatgatctca gtttcaaact tggtggcatg ttcgtgcatg cgcgccatta ataacggacc
    52681 ggtcagatcg tttggatcac caggccagtt ttccacttcc gtggtggtgg tcagttggcc
    52741 gcctttttcc atgccggtaa tcagcacagg ttgcaggttg gcgcgcgccg cgtagacagc
    52801 agcggtgtat cccgccgggc ctgaacccag gataagcagt ttactgtgtt tggtcgtgcc
    52861 catgagatcc ccatagttgt tggcagacaa tgggcaggat tgtagggaat ttacagacgt
    52921 aaaaaaagag tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc gataagcagg
    52981 tcaaattctc ccgtcattat cacctctgct acttaaattt cccgctttat aagccgatta
    53041 aatgatgaat aaacgcccct gttaatgaat atctggcatg ttgtactaaa aattgatgtt
    53101 ttgctttgac aatcccctgg tgttttgcga aaacattcga ggaagaaaaa aaacagtatt
    53161 cttatatgcg cataaccatg tatgtaaata ccatgtttac cgtgctagtg aaatctacgt
    53221 atggcgtgga cagacgccat tcgtgatgtc gatagctgcc acaaggcaac ggtcttctca
    53281 ccgtagaccc aggcattgcg cgccgtgaat cttcatgatt tcggtctatc gtgacgggta
    53341 gcgactctga acagtgatgt ttcagggtca gacaggagta gggaaggaat acagagagac
    53401 aataataatg gtagatagca agaagcgccc tggcaaagat ctcgaccgta tcgatcgtaa
    53461 cattcttaat gagttgcaaa aggatgggcg tatttctaac gtcgagcttt ctaaacgtgt
    53521 gggactttcc ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac aagggtttat
    53581 tcagggctat acggcgctgc ttaaccccca ttatctggat gcatcacttc tggtattcgt
    53641 tgagattact ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata ccgctgtaca
    53701 aaaacttgaa gaaattcagg agtgtcattt agtatctggt gatttcgact acctgttgaa
    53761 aacacgcgtg ccggatatgt cagcttaccg taagttgctg ggggaaaccc tgctgcgtct
    53821 gcctggcgtc aatgacacac ggacatacgt tgttatggaa gaagtcaagc agagtaatcg
    53881 tctggttatt aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt ttgattacac
    53941 tcctgttaat ccatacagca acagtactgg ggtaacctgg tactgttgtc cgttttagca
    54001 tcgggcagga aaagcctgta acctggagag cctttcttga gccaggaata cactgaagac
    54061 aaagaagtca cattgacaaa gttaagtagc ggccgccgcc ttctggaggc gttgctgatc
    54121 cttattgtcc tgtttgccgt ctggttgatg gctgccttac taagctttaa cccttcggac
    54181 cccagctggt cgcaaacggc ctggcatgaa cctatccata atttaggtgg gatgcccggt
    54241 gcgtggttgg cagatacgct gttctttatt tttggcgtga tggcttacac cattcccgtc
    54301 attattgtcg gcggttgttg gtttgcctgg cgtcatcagt ccagcgacga atacattgat
    54361 tattttgccg tttcgctacg catcattggc gttttggcgc tcatccttac ctcctgtggt
    54421 ctggcggcaa tcaacgctga cgatatctgg tattttgcct ccggtggcgt cattggcagc
    54481 ttactaagca ctacgctaca accactgcta cacagtagcg ggggaactat tgcgctgctc
    54541 tgcgtttggg cagcgggcct gacgttgttc accggttggt catgggtgac cattgctgaa
    54601 aaactcggcg gctggatttt aaacattctc accttcgcca gtaatcgtac ccgtcgcgat
    54661 gatacctggg tcgatgaaga tgagtatgaa gacgacgaag agtatgaaga tgaaaatcac
    54721 ggcaaacagc atgaatcacg ccgtgcccgt attcttcgcg gcgcgctagc gcgtcgtaaa
    54781 cggttggcgg aaaaattcat taatccgatg gggcggcaaa cagacgctgc gttgttctcc
    54841 ggtaagcgga tggatgatga cgaagagatt acctacactg cacgcggtgt ggctgccgac
    54901 ccggacgacg tcctattttt gggcaatcgt gcaacgcagc cagaatatga cgaatacgat
    54961 ccattattaa acggtgcgcc aattaccgaa cctgtcgctg tagcagctgc tgctaccacg
    55021 gcgacacaaa gctgggctgc gccggttgaa cctgtgactc agacgccgcc tgttgcctct
    55081 gttgatgttc caccttcgca acctacagta gcctggcagc ctgtaccggg tccacaaacg
    55141 ggagagccgg ttattgctcc tgcaccggaa ggttacccac agcagtcaca atatgcgcag
    55201 cctgcagtgc aatataatga gccgctgcaa caaccagtac agccgcagca gccgtattat
    55261 gcacctgcag ctgaacaacc tgcgcaacaa ccttactacg ctcctgcggc ggaacaacct
    55321 gttcagcaac cgtattacgc aactgcgccg gaacagcctg cgcaacagcc gtattatgcc
    55381 cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca gcaatccatt
    55441 tttgctccac agtctacata ccagactgag caaacttatc agcagccagc cgctcaggag
    55501 ccgttgtacc aacagccgca acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt
    55561 gtagaagaga caaaacccgc gcgtccgccg ctttactatt ttgaagaagt ggaagagaag
    55621 cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattccaga accggttaaa
    55681 gaaccagaac cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt acctccagta
    55741 gaagccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac actggcgacg
    55801 ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt
    55861 ccgcaggtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca
    55921 actcgtcgtg aattggcgtc ttacggtatt aagctgccct cacagcgtgc ggcggaagaa
    55981 aaagcccgtg aagcccggcg caatcagtat gattctggcg atcagtataa cgatgatgaa
    56041 atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc
    56101 tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga tgctgcggca
    56161 gaggctgaac tggctcgtca gtttacgcaa actcaacaac aacgttattc cggcgaacaa
    56221 ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat gaaagcgttg
    56281 ctggatgatg gtccacacga accgctgttt acgccaattg ttgaacctgt acagcagccg
    56341 caacaaccgg ttgcaccaca gcagcagtat cagcagccac aacagccggt tgcgccacag
    56401 ccgcagtatc agcagccaca acagcaggtt gcgccgcagc cacaatatca gcagccgcaa
    56461 caaccggttg cgccacagca gcaatatcag cagccgcaac aaccagttgc gccgcagccg
    56521 cagtatcaac agccgcaaca accggttgca ccgcagccgc agtatcagca gccacaacag
    56581 ccagttgcgc cacagccgca ggataccctg cttcatccgc tgttgatgcg taatggcgac
    56641 agccgtccgt tgcacaaacc gacgacgccg ctgccttctc tggatttgct gacaccgccg
    56701 ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggcgcg tctggtggaa
    56761 gcgcgtctgg ctgatttccg tattaaagcc gatgtcgtta attactctcc ggggccagtt
    56821 atcactcgct ttgaacttaa cctggctcct ggcgtaaaag cggcgcgcat ttctaacttg
    56881 tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga agttattcct
    56941 ggcaaaccct atgtaggtct ggagttacca aataaaaaac gacaaaccgt ttatctgcgc
    57001 gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt ggtgctgggt
    57061 aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca cttgttggtt
    57121 gcggggacta ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct gagcatgctt
    57181 tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat gctggagctt
    57241 tcggtttatg aaggcattcc gcacctgtta acggaagtcg ttactgatat gaaagatgcc
    57301 gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc ggtataaact gatgtctgcg
    57361 ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga tcgcatgatg
    57421 cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca gcatccggtg
    57481 ctgaaaaaag aaccatacat tgtcgtgttg gttgacgaat ttgccgacct gatgatgacg
    57541 gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt
    57601 atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg tctgattaaa
    57661 gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc acgtaccatt
    57721 cttgatcagg ctggcgcgga atcactgctg ggtatggggg atatgctcta ctctgggccg
    57781 aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga agttcatgcc
    57841 gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat cacctccgac
    57901 agcgaaagcg aaggtggtgc gggtggtttc gatggcgctg aagaactgga tccgttgttc
    57961 gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg cgtacagcgt
    58021 cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga agcgcagggg
    58081 attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggccccacc gccgtttgac
    58141 taattaatgc attgccggat aaggcgcggt agcgtcgcat ccggcactct atcaactgaa
    58201 aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag agtagaggga
    58261 actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg ctatcacctg
    58321 tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa
    58381 aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag tgactgacgg
    58441 tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt
    58501 caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta aaacactgtg
    58561 gttctataac ccgttcgttg agcaagctac ggcaacctgg ctgaaagatg ccaccggtaa
    58621 tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt acaatatcaa
    58681 acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt
    58741 caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg agcaggacga
    58801 tcagcgcagc agttatcagc tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt
    58861 taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag
    58921 tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc gcgcgtatgc
    58981 ggccagaaaa tttagcacag tatatcggtc agcaacattt gctggctgcg gggaagccgt
    59041 tgccgcgcgc tatcgaagcc gggcatttgc attctatgat cctctggggg ccaccgggta
    59101 ccggcaaaac aaccctcgct gaagtgattg cccgctatgc gaacgctgat gtggaacgta
    59161 tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa
    59221 accgcaatgc aggtcgccgc actattcttt ttgttgacga agttcaccgt ttcaacaaaa
    59281 gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt attggcgcaa
    59341 ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc cgtgtttatc
    59401 tgttgaaatc cctgagtaca gaggatattg agcaagtact aactcaggcg atggaagaca
    59461 aaacccgtgg ctatggtggt caggatattg ttctgccaga tgatacacga cgtgccattg
    59521 cagaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg atggcggata
    59581 tggccgaagt ctatgatagc ggtaagcggg tcctgaagcc tgaattactg accgaaatcg
    59641 ccggtgaacg tagcgcccgc tttgataaca aaggcgatcg cttttacgat ctaatttccg
    59701 cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctgtactgg tatgcgcgaa
    59761 ttattaccgc tggtggcgat ccgttatatg tcgcgcgtcg ctgtctggcg attgcgtctg
    59821 aagacgtcgg taatgccgat ccacgggcga tgcaggtggc aattgcggcc tgggattgct
    59881 ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg
    59941 cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agccgcgctg gccgatgctc
    60001 gcgaacgccc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg aaattaatga
    60061 aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac gcttatgctg
    60121 ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc ccgacaaaca
    60181 ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata
    60241 gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact gtatccctgt
    60301 ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata agcatgctcg
    60361 atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca cgccggggct
    60421 ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca
    60481 aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc caggcgaaag
    60541 cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc gaagagctgg
    60601 atgcagcaaa agccgagcta gatgctttac aggctgaaat tcgcgatatc gcgctgacca
    60661 tccctaacct gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac aacgttgaag
    60721 tcagccgctg gggtaccccg cgtgagtttg actttgaagt tcgtgaccac gtgacgctgg
    60781 gtgaaatgca ctctggcctc gactttgcag ctgcagttaa gctgactggt tcccgctttg
    60841 tggtaatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt atgctggatc
    60901 tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt aaccaggaca
    60961 cgctgtacgg tacgggtcag ctgccgaaat ttgctggcga tctgttccat actcgtccgc
    61021 tggaagaaga agcagacacc agtaactatg cgctgatccc aacggcagaa gttccgctga
    61081 ctaacctggt gcgcggtgaa atcatcgatg aagatgatct gccaattaag atgaccgccc
    61141 acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt ggtctgatcc
    61201 gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccagaa gactcaatgg
    61261 cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcagttgctg ggcctgccgt
    61321 accgtaaaat catcctttgc actggcgaca tgggctttgg cgcttgcaaa acttacgacc
    61381 tggaagtatg gatcccggca cagaacacct accgtgagat ctcttcctgc tccaacgttt
    61441 gggatttcca ggcacgtcgt atgcaggcac gttgccgcag caagtcggac aagaaaaccc
    61501 gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg gttgcagtaa
    61561 tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg cgtccgtata
    61621 tgaacggact ggaatatatt ggctaatacc caatttttct gaatctaaaa agcgccttta
    61681 gggcgctttt tttgtctccc tttgataccg aacaataatt actcctcact tacacgtaat
    61741 actactttcg agtgaaaatc tacctatctc tttgattttc aaattattcg atgtatacaa
    61801 gcctatatag cgagctgcta tagaaataat tacacaatac ggtttgttac tggaatcaat
    61861 cgtgatcaag cttgagtgag ccattatgaa aacgaaaatc cctgatgcgg tattggctgc
    61921 tgaggagagt cgccgtggtt tggtaaaaac gacagcgatc ggcggcctgg caatggccag
    61981 cagcgcatta acattacctt ttagtcggat tgcgcacgct gtcgatagcg ccattccaac
    62041 aaaatcagac gaaaaggtta tctggagcgc ctgtacagtt aactgtggta gtcgctgccc
    62101 gctacgtatg cacgtcgtgg acggtgaaat caaatatgtc gaaacggaca ataccggcga
    62161 tgacaattac gacggcctgc actaggttcg cgcctgcctg cgtgggcgtt ccatgcgtcg
    62221 ccgtgtctac aatccggacc gcctgaaata tccgatgaaa cgagtcgggg cgcgcggtga
    62281 aggcaaattc gagcgcatta gctgggaaga agcctacgac atcatcgcga ccaatatgca
    62341 gcgcctgatc aaagagtacg gcaacgagtc tatctatctg aactatggca ccggtacgct
    62401 gggcggcacc atgacccgct cctggccgcc gggaaatacc ctggtcgcgc gcctgatgaa
    62461 ctgctgcggc ggttatctta accattacgg tgactactct tccgcgcaaa ttgcggaagg
    62521 tttgaaccat acctacggcg gctgggcaga tggtaacagc ccgtcggata tcgaaaacag
    62581 taaattggta gtgctgtttg gtaataaccc aggcgaaacg cgaatgagtg gcggtggggt
    62641 gacttactat cttgaacagg cacgccagaa atctaatgct cgcataatca tcatcgatcc
    62701 gcgctatacc gacaccggtg ccgggcgcga agatgagtgg atccctattc gtccaggaac
    62761 agatgccgca ctggttaacg gtctggcgta cgtcatgatc actgaaaacc tggtggatca
    62821 ggcattcctc gataaatatt gcgttggcta tgacgagaaa accctgcccg ccagtgcgcc
    62881 gaaaaatggt cattataaag cttatattct gggtgaaggg ccagatggcg tggctaaaac
    62941 accggaatag gcctcgcaaa tcaccggtgt tccggcagac aaaatcatca aactggctcg
    63001 tgaaatcggc agtaccaaac cggcgtttat cagccaggga tggggcccgc agcgtcacgc
    63061 taacggtgaa atcgcaaccc gtgctatctc gatgctggcg attctgaccg gtaacgttgg
    63121 tattaatgga gggaacagcg gcgcgcgtga aggttcatac agcttaccgt ttgtccgtat
    63181 gccgaccttg gaaaacccga tccagaccag catttcgatg tttatgtgga ccgatgccat
    63241 tgaacgtggc ccggaaatga cggcgctgcg tgatggtgtg cgcgggaaag ataagctgga
    63301 tgtgccgatc aaaatgatct ggaactatgc cggtaactgc ctgattaacc agcattctga
    63361 aatcaaccgt acccatgaaa tcctccagga tgataagaag tgcgagctga ttgtggttat
    63421 cgactgccac atgacctcat cggcgaaata tgctgacatc ctgctgcctg actgcaccgc
    63481 ttccgaacag atggactttg cactggatgc atcctgcggg aatatgtctt acgtgatttt
    63541 caacgatcag gtgattaaac cgcgctttga atgtaagacc atctatgaaa tgaccagcga
    63601 actggcaaaa cgtcttggcg ttgagcaaca gtttactgaa ggccgtaccc aggaagagtg
    63661 gatgcggcat ctgtatgccc agtcgcggga agcgattcct gaactgccaa cgtttgaaga
    63721 gttccgcaag caggggatct ttaaaaagcg cgacccacaa gggcatcacg ttgcttataa
    63781 agccttccgt gaagatccgc aggcaaaccc actgactacg ccatcgggca aaattgagat
    63841 ttattcgcag gcgctggctg acattgccgc tacctgggaa ttgccagaag gcgatgtgat
    63901 cgatccactg ccgatctaca cgccgggctt tgaaagttat caggatccgc tgaacaaaca
    63961 gtatccgctg cagcttacag gtttccacta taaatctcgc gttcactcaa cttacggcaa
    64021 cgttgatgtg ctgaaagcgg cttgccgtca ggaaatgtgg atcaacccgc ttgatgccca
    64081 aaaacgcggt atccacaacg gcgataaagt caggatcttt aacgatcgtg gtgaggttca
    64141 tattgaggcg aaagtgacgc cacgaatgat gccgggcgtg gtcgcactgg gtgaaggcgc
    64201 ctggtatgac ccggatgcaa aacgtgtcga taagggcggt tgtatcaacg tactgaccac
    64261 tcaacgtccg tctcctctcg ctaaggggaa tccgtcacat acaaaccttg ttcaggttga
    64321 aaaggtgtaa ggagtaaccg atgacaaccc agtatggatt ttttattgat tccagccgtt
    64381 gcaccggttg caaaacctgc gagctggctt gtaaagacta caaagatttg acgccagaag
    64441 tcagtttccg ccgcatttat gaatatgctg gcggcgactg gcaggaagat aacggtgtct
    64501 ggcaccagaa cgtgtttgcc tactatctgt cgatttcgtg caaccactgc gaagatccgg
    64561 cttgtactaa agtctgcccg agcggtgcga tgcataaacg cgaagatggt tttgttgtgg
    64621 tcgatgaaga tgtatgcatt ggctgccatt actgtcatat ggcttgcccg tatggcgcac
    64681 cgcaatataa cgaaacgaaa ggccatatga ccaaatgcga tggttgttat gaccgtgttg
    64741 cggagggtaa aaagccgatc tgtgttgaat cttgcccgct gcgggcgctg gatttcggcc
    64801 ctatcgacga gctgcgtaaa aaacatggcg atctggcggc cgttgcgccg ttgccgcgag
    64861 ctcactttac caaaccgaat attgtgatca aacccaatgc caatagccgc ccgacagggg
    64921 ataccactgg ctatctggca aacccgaagg aggtgtgaga tgggaagtgg atggcatgaa
    64981 tggccgctga tgatcttcac ggtcttcggg caatgtgtgg cgggtggttt tatcgttctg
    65041 gctttggcgc tgctcaaagg cgacctgcga gcagaagccc agcagcgtgt tatcgcctgc
    65101 atgtttggct tatgggtact gatgggcatt ggctttatcg cctctatgct ccatcttggt
    65161 tcaccgatgc gcgcctttaa ctcgctcaac cgggtagggg cttcagcact cagtaacgaa
    65221 atcgccagcg gttcgatctt ttttgctgta ggcggcatcg gctggttgct ggcaatgctg
    65281 aaaaagctgt caccggcatt gcgtacgcta tggctggtgg tgacgatggt tcttggcgtc
    65341 gtctttgtct ggatgatggt gcgtgtgtat aacagcattg ataccgttcc gacctggtac
    65401 agcatctgga cgccgatggg cttcttcctg acgatgttca tgggcggccc gttactgggt
    65461 tacctgttgc tgagtctggc tggcgtcgat ggctgggcga tgcgtctgct gccagccatt
    65521 tctgtactgg cactggtggt gagtggcgtg atgtcagtga tgcagggcac agagctggca
    65581 actattcata gttctgtgca gcaggccgca gcgctggtgc cggactatgg tgcgctgatg
    65641 tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt ggattgcacc acagctaaaa
    65701 ggttatcagc ctgcggttcc gctactatcc gtctcattca ttctgcttct ggcaggggaa
    65761 ttaatcggtc gcggcgtatt ctatggtttg catatgaccg tggggatggc cgtcgcaagc
    65821 taatcagtaa caaccggggt ttcggccccg gttctctttt atttctgctt cgttaacgtg
    65881 tcataactgg tcatcaaatt acgataatca ggaatatggt tagagaacaa cgtcgccagt
    65941 ccttcaatat cattacgcca gtcacggtgc agctcgcaag ccacgccgaa ccatgtcatc
    66001 aattgcgcac cagcttgcga catgcgatcc cacgccgaat gccgggtaat ttcattaaag
    66061 gtccctgatg catcagtcac gacaaagaca tcaaaccctt cttcaatggc tgaaagcgcc
    66121 gggaatgcca cgcaaacttc ggttaccaca ccggcaataa ttaactgttt tttacctgtc
    66181 gctttgactg cttttacaaa atcttcgtta tcccaggcgt taatattgcc cgggcgagca
    66241 atgtaaggtg catcggggaa ttgtgctttc agttctagaa ctaatgggcc gttaggaccg
    66301 gtttcaaaac tggtggtaag aatagttggt aagttgaaat acttagccag gtcacccagc
    66361 gccagcacgt tatttttaaa cttatcgggt tcgatatccc gtacaaggga aagtaaacca
    66421 gcctggtgat caacaagcaa aacggcagca tcatttttat caagacgaac atacggtttg
    66481 gtcatcctct tctcctttcg agagtcagtg gtacgtctga aatcggtcag acgcgatgca
    66541 ttgctctgaa agcatagacg ggaaatatga gtttgctgtg accatgaaat ttttcgactg
    66601 aacgaagcgt atgaaatttg tgttagatca ataaaaacaa tcagatgaga ttaatcatct
    66661 cttcagcatt agcgttatga atgagtaata taaatcgata ataccgccgc tgcgatatgc
    66721 gcgtattttc acccattgac aatgtttttg gcggtggcat gatgcgcatg aaatttgaac
    66781 ttcctcacgg ttttaattca tgtccacgta tacccggcct gtcatgcttt tactgtctgg
    66841 cctgcttttg ttgactctgg cgatcgcggt gttaaataca ctcgtgccgc tttggctcgc
    66901 ccaggaacac atgtccacat ggcaggtagg cgttgtcagc tcatcctatt ttaccggcaa
    66961 ccttgtcggt acattgctga cagggtatgt cattaaacgc attggcttta accgcagcta
    67021 ttatctgtcc tccttcattt ttgctgctgg ctgtgccggc cttggcctga tgattggttt
    67081 ctggagctgg ttggcgtggc gttttgtcgc gggcgtcggc tgtgccatga tttgggtggt
    67141 tgttgagagc gcgctgatgt gcagtgggac gtcacgtaac cgtgggcgtt tgcttgctgc
    67201 gtatatgatg gtttattacg tgggaacgtt tttaggccag ttactggtca gcaaagtctc
    67261 aaccgagctg atgagcgtat tgccgtgggt tacaggtttg acattggcag ggatcttacc
    67321 gctgttgttt acgcgtgtcc tgaatcagca ggctgaaaac catgattcga cgtcaattac
    67381 ggcaatgcta aaactccgtc aggcgcggct tggcgtgaat ggctgcatta tctcaggaat
    67441 cgttctggga tctctatatg gcctgatgcc tctgtacctc aatcacaaag gggtgagcaa
    67501 tgccagtatt ggtttctgga tggcggtact ggtcagtgcg ggtatcctcg gacaatggcc
    67561 gattggacgt ctggcggata agtttggtcg actgctggtt ttgcgtgttc aggtctttgt
    67621 cgtcattctc ggcagtattg cgatgcttag ccaggcggcg atggccccag cgttattcat
    67681 cctcggtgcc gctggcttta cgctatatcc ggtggcgatg gcctgggctt gtgagaaagt
    67741 tgaacatcat caactggtgg cgatgaacca agccttactg ttgagctata ccgtgggaag
    67801 tctgcttggc ccgtcattta ccgctatgct aatgcagaat ttctccgata atttattgtt
    67861 tatcatgatc gccagcgtat cgtttatcta tttgctgatg ctgcttcgca acgccggtca
    67921 tacgccgaaa cccgttgctc acgtgtaaat gaattcaagc aaagtgtgaa cttactgttt
    67981 cacactctgc ttttttgttt cttctatctg acttgctttt ttccaaattt tattcgttta
    68041 aaaataaaat gtgcagcagg ttataatttt gcatttcgct atttccgcac ttcttatttg
    68101 ccgcgcataa tccctcgttt tacagatgcc cctttaattt tggcgaagga tttgtctatg
    68161 gctgggaatg ttcaggaagt aagcgcccca tca
//
LOCUS       DAJVQH010000006        66408 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJVQH010000006 DAJVQH010000000
VERSION     DAJVQH010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.006"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..2288)
                     /gene="glgP"
                     /locus_tag="OQM87_000480"
     CDS             complement(<1..2288)
                     /gene="glgP"
                     /locus_tag="OQM87_000480"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCS1506743.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRS"
     gene            complement(2307..3740)
                     /gene="glgA"
                     /locus_tag="OQM87_000481"
     CDS             complement(2307..3740)
                     /gene="glgA"
                     /locus_tag="OQM87_000481"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCS1506744.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3740..5035)
                     /gene="glgC"
                     /locus_tag="OQM87_000482"
     CDS             complement(3740..5035)
                     /gene="glgC"
                     /locus_tag="OQM87_000482"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCS1506745.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5053..7026)
                     /gene="glgX"
                     /locus_tag="OQM87_000483"
     CDS             complement(5053..7026)
                     /gene="glgX"
                     /locus_tag="OQM87_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCS1506746.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7023..9209)
                     /gene="glgB"
                     /locus_tag="OQM87_000484"
     CDS             complement(7023..9209)
                     /gene="glgB"
                     /locus_tag="OQM87_000484"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCS1506747.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDASHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9482..10585)
                     /gene="asd"
                     /locus_tag="OQM87_000485"
     CDS             complement(9482..10585)
                     /gene="asd"
                     /locus_tag="OQM87_000485"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1506748.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10777..11370
                     /gene="yhgN"
                     /locus_tag="OQM87_000486"
     CDS             10777..11370
                     /gene="yhgN"
                     /locus_tag="OQM87_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCS1506749.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11427..12767)
                     /gene="gntU"
                     /locus_tag="OQM87_000487"
     CDS             complement(11427..12767)
                     /gene="gntU"
                     /locus_tag="OQM87_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1506750.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMDICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLN"
     gene            complement(12771..13298)
                     /gene="gntK"
                     /locus_tag="OQM87_000488"
     CDS             complement(12771..13298)
                     /gene="gntK"
                     /locus_tag="OQM87_000488"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS1506751.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13437..14432)
                     /gene="gntR"
                     /locus_tag="OQM87_000489"
     CDS             complement(13437..14432)
                     /gene="gntR"
                     /locus_tag="OQM87_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCS1506752.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14656..15351)
                     /gene="yhhW"
                     /locus_tag="OQM87_000490"
     CDS             complement(14656..15351)
                     /gene="yhhW"
                     /locus_tag="OQM87_000490"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCS1506753.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15474..16511)
                     /locus_tag="OQM87_000491"
     CDS             complement(15474..16511)
                     /locus_tag="OQM87_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1506754.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            16844..17332
                     /gene="yhhY"
                     /locus_tag="OQM87_000492"
     CDS             16844..17332
                     /gene="yhhY"
                     /locus_tag="OQM87_000492"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1506755.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17391..19113)
                     /gene="ggt"
                     /locus_tag="OQM87_000493"
                     /pseudo
     CDS             complement(17391..19113)
                     /gene="ggt"
                     /locus_tag="OQM87_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            19233..19673
                     /locus_tag="OQM87_000494"
     CDS             19233..19673
                     /locus_tag="OQM87_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCS1506756.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19660..20403)
                     /gene="ugpQ"
                     /locus_tag="OQM87_000495"
     CDS             complement(19660..20403)
                     /gene="ugpQ"
                     /locus_tag="OQM87_000495"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1506757.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20400..21390)
                     /locus_tag="OQM87_000496"
                     /pseudo
     CDS             complement(20400..21390)
                     /locus_tag="OQM87_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21392..22237)
                     /gene="ugpE"
                     /locus_tag="OQM87_000497"
     CDS             complement(21392..22237)
                     /gene="ugpE"
                     /locus_tag="OQM87_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCS1506758.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22234..23121)
                     /gene="ugpA"
                     /locus_tag="OQM87_000498"
     CDS             complement(22234..23121)
                     /gene="ugpA"
                     /locus_tag="OQM87_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCS1506759.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23219..24535)
                     /gene="ugpB"
                     /locus_tag="OQM87_000499"
     CDS             complement(23219..24535)
                     /gene="ugpB"
                     /locus_tag="OQM87_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCS1506760.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(24932..25645)
                     /gene="livF"
                     /locus_tag="OQM87_000500"
     CDS             complement(24932..25645)
                     /gene="livF"
                     /locus_tag="OQM87_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCS1506761.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSSYLGG"
     gene            complement(25647..26414)
                     /gene="livG"
                     /locus_tag="OQM87_000501"
     CDS             complement(25647..26414)
                     /gene="livG"
                     /locus_tag="OQM87_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCS1506762.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26411..27688)
                     /gene="livM"
                     /locus_tag="OQM87_000502"
     CDS             complement(26411..27688)
                     /gene="livM"
                     /locus_tag="OQM87_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCS1506763.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27685..28611)
                     /gene="livH"
                     /locus_tag="OQM87_000503"
     CDS             complement(27685..28611)
                     /gene="livH"
                     /locus_tag="OQM87_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCS1506764.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28659..29768)
                     /gene="livK"
                     /locus_tag="OQM87_000504"
     CDS             complement(28659..29768)
                     /gene="livK"
                     /locus_tag="OQM87_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCS1506765.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30192..30575
                     /gene="panM"
                     /locus_tag="OQM87_000505"
     CDS             30192..30575
                     /gene="panM"
                     /locus_tag="OQM87_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCS1506766.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30774..31877)
                     /gene="livJ"
                     /locus_tag="OQM87_000506"
     CDS             complement(30774..31877)
                     /gene="livJ"
                     /locus_tag="OQM87_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCS1506767.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32148..33002)
                     /gene="rpoH"
                     /locus_tag="OQM87_000507"
     CDS             complement(32148..33002)
                     /gene="rpoH"
                     /locus_tag="OQM87_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCS1506768.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33247..34305)
                     /gene="ftsX"
                     /locus_tag="OQM87_000508"
     CDS             complement(33247..34305)
                     /gene="ftsX"
                     /locus_tag="OQM87_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCS1506769.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34298..34966)
                     /gene="ftsE"
                     /locus_tag="OQM87_000509"
     CDS             complement(34298..34966)
                     /gene="ftsE"
                     /locus_tag="OQM87_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCS1506770.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(34969..36465)
                     /gene="ftsY"
                     /locus_tag="OQM87_000510"
     CDS             complement(34969..36465)
                     /gene="ftsY"
                     /locus_tag="OQM87_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCS1506771.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36615..37211
                     /gene="rsmD"
                     /locus_tag="OQM87_000511"
     CDS             36615..37211
                     /gene="rsmD"
                     /locus_tag="OQM87_000511"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCS1506772.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37201..37470
                     /locus_tag="OQM87_000512"
     CDS             37201..37470
                     /locus_tag="OQM87_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCS1506773.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37473..37832)
                     /locus_tag="OQM87_000513"
     CDS             complement(37473..37832)
                     /locus_tag="OQM87_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCS1506774.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            37973..38599
                     /locus_tag="OQM87_000514"
     CDS             37973..38599
                     /locus_tag="OQM87_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCS1506775.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38673..40871
                     /gene="zntA"
                     /locus_tag="OQM87_000515"
     CDS             38673..40871
                     /gene="zntA"
                     /locus_tag="OQM87_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCS1506776.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(40973..41218)
                     /gene="tusA"
                     /locus_tag="OQM87_000516"
     CDS             complement(40973..41218)
                     /gene="tusA"
                     /locus_tag="OQM87_000516"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCS1506777.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41439..42104
                     /locus_tag="OQM87_000517"
     CDS             41439..42104
                     /locus_tag="OQM87_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCS1506778.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42177..42734
                     /gene="dcrB"
                     /locus_tag="OQM87_000518"
     CDS             42177..42734
                     /gene="dcrB"
                     /locus_tag="OQM87_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCS1506779.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42738..43955)
                     /locus_tag="OQM87_000519"
                     /pseudo
     CDS             complement(42738..43955)
                     /locus_tag="OQM87_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44087..45136
                     /locus_tag="OQM87_000520"
     CDS             44087..45136
                     /locus_tag="OQM87_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1506780.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45191..45778
                     /gene="acpT"
                     /locus_tag="OQM87_000521"
     CDS             45191..45778
                     /gene="acpT"
                     /locus_tag="OQM87_000521"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCS1506781.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            45889..47463
                     /gene="nikA"
                     /locus_tag="OQM87_000522"
     CDS             45889..47463
                     /gene="nikA"
                     /locus_tag="OQM87_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCS1506782.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47463..48407
                     /gene="nikB"
                     /locus_tag="OQM87_000523"
     CDS             47463..48407
                     /gene="nikB"
                     /locus_tag="OQM87_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCS1506783.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48404..49237
                     /gene="nikC"
                     /locus_tag="OQM87_000524"
     CDS             48404..49237
                     /gene="nikC"
                     /locus_tag="OQM87_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCS1506784.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLILGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49237..50001
                     /gene="nikD"
                     /locus_tag="OQM87_000525"
     CDS             49237..50001
                     /gene="nikD"
                     /locus_tag="OQM87_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCS1506785.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            49998..50804
                     /gene="nikE"
                     /locus_tag="OQM87_000526"
     CDS             49998..50804
                     /gene="nikE"
                     /locus_tag="OQM87_000526"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCS1506786.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            50810..51211
                     /gene="nikR"
                     /locus_tag="OQM87_000527"
     CDS             50810..51211
                     /gene="nikR"
                     /locus_tag="OQM87_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCS1506787.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51331..51690)
                     /locus_tag="OQM87_000528"
     CDS             complement(51331..51690)
                     /locus_tag="OQM87_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCS1506788.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51687..51962)
                     /locus_tag="OQM87_000529"
     CDS             complement(51687..51962)
                     /locus_tag="OQM87_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCS1506789.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(52021..53145)
                     /locus_tag="OQM87_000530"
     CDS             complement(52021..53145)
                     /locus_tag="OQM87_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1506790.1"
                     /translation="MRQLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53145..55880)
                     /gene="rbbA"
                     /locus_tag="OQM87_000531"
     CDS             complement(53145..55880)
                     /gene="rbbA"
                     /locus_tag="OQM87_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCS1506791.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(55877..56944)
                     /locus_tag="OQM87_000532"
     CDS             complement(55877..56944)
                     /locus_tag="OQM87_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1506792.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57310..58932)
                     /locus_tag="OQM87_000533"
     CDS             complement(57310..58932)
                     /locus_tag="OQM87_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1506793.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINKNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59194..60783)
                     /locus_tag="OQM87_000534"
     CDS             complement(59194..60783)
                     /locus_tag="OQM87_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1506794.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEESQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61167..62198
                     /locus_tag="OQM87_000535"
                     /pseudo
     CDS             61167..62198
                     /locus_tag="OQM87_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62732..63496)
                     /locus_tag="OQM87_000536"
     CDS             complement(62732..63496)
                     /locus_tag="OQM87_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCS1506795.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSANLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNGETYAICSEVLLYD"
     gene            63743..64504
                     /locus_tag="OQM87_000537"
     CDS             63743..64504
                     /locus_tag="OQM87_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1506796.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSLQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64611..65111)
                     /locus_tag="OQM87_000538"
     CDS             complement(64611..65111)
                     /locus_tag="OQM87_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506797.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65262..>66408)
                     /locus_tag="OQM87_000539"
     CDS             complement(65262..>66408)
                     /locus_tag="OQM87_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1506798.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDQK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16128 a        17826 c        16303 g        16151 t
ORIGIN      
        1 tagctgcgat aatccgccag tacctggtag tgatcgccga agttgatcag cgaatcaacc
       61 agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat ttgcgtcagc
      121 acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc ctgacgacgc
      181 agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc accgacatga
      241 tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc gttaagcgca
      301 aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg ttcagacaga
      361 tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat gaacacgact
      421 ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc attgatcaaa
      481 tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa aatattcacg
      541 cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt aatcacatgc
      601 aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac atcgaacaac
      661 gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag acgctttttg
      721 ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca gtgttgttgc
      781 agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc gtccagcacg
      841 gctgaaagcg atgggttcgc taccgccagc cagcgacgcg gcgtcacacc gttggtgacg
      901 ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg caccatcaga
      961 ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc cagccaggcc
     1021 atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag cagatcggta
     1081 tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc aaagatgatc
     1141 tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc cagcgcctcg
     1201 ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa cgcgtcgtcc
     1261 cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga cagtaccgga
     1321 tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt tttatgtaac
     1381 tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccaggaagta ttcctgacgc
     1441 aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg agatacgttc
     1501 tcggaatggt ttttatcttc cactgcagcg aagtagtcac cctggttgaa tttaccgagg
     1561 ttaatttcgc tactggcttg cgcactccac aaacgcagcg tgttggtcgc gtcggtgtcg
     1621 taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc aatccagcgc
     1681 gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg cgtgttgtgg
     1741 cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc tttctggcta
     1801 ccgttaacga tgttctgctt gaacataccg tagtcatagc gaatgccgta accgcgcccc
     1861 ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc caggccaccg
     1921 ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa ccccatcgct
     1981 tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt ggagagcgta
     2041 cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg cgacaactgg
     2101 gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa taacgttgcg
     2161 ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa catcagcttg
     2221 taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata tgtaaacgga
     2281 gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc acggtacgac
     2341 ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac aaaccgccac
     2401 agtgaaggac gggaccacag tacaaaagca cgccgaatag cccgtaacag cgaccaggca
     2461 ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc aagagaacag
     2521 tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt accgtacttc
     2581 aatccataaa gttgcgttaa gccacacggt tcaaaacggc tgggcaccag aatgacgtcc
     2641 gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac gcccacctga
     2701 ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc gcccgcgccg
     2761 agtagcgcca gctgcccgcc ctgctccaga agccccggta aggcttccag caccaggtcg
     2821 agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt atcgtcaacc
     2881 ttaagcccca ttgcgatttg taactggctc ttattttccg ctttatcttc caacgtatcg
     2941 cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt ctcgtccacg
     3001 ccgttcagta cgcctgaaag acgcccttca cggtgacgct gttgcaacag accttccata
     3061 ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac cgccgtaatg
     3121 tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa ttccagccca
     3181 tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc ataaaacatg
     3241 ccttgatagg ccaggttgtg cacagtaaac accgacttcg caggacgccc gcgcgccgcc
     3301 agatacgcag gcgcaagacc tgcatgccag tcgtgcgcat gcaccacatc aggacgccag
     3361 aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc aaaacgcaat
     3421 acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg acgatcatag
     3481 agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa caacagcgtg
     3541 atatgcccgg cgaaagtatc acgacgggat actacctgcg catcggtcac gccacggcga
     3601 atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat ttgtgctgcg
     3661 ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa catctctgaa
     3721 catacatgta aaacctgcat tatcgctcct gtttatgccc taacttccgt agcatttcgc
     3781 gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct tcctctgcgt
     3841 tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg cagcggcgca
     3901 gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca atgttgcaga
     3961 atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa ccggagatca
     4021 cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag cgatcctgca
     4081 cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg cgatcgtaca
     4141 tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa gcttccagcg
     4201 tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa gagagcggga
     4261 acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct ttgccaaagt
     4321 cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga tagtcggcgt
     4381 caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc attgacggtg
     4441 ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc gccataacgc
     4501 caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa cgtgcgcctt
     4561 tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg tcgcccgcca
     4621 ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg gtgaccgcat
     4681 ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc agcagatcga
     4741 caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc tgcaccagag
     4801 tgtgggactg gtactgggtg atcacgccca tacgacggat cccagagttg atgcagttag
     4861 acagcgcaaa gtcaataatg cggaacttac cgccgaagtg tacggccggt tttgctcgct
     4921 tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg gcaacagatt
     4981 tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta accatgacta
     5041 actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct gccagacaac
     5101 cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact ccccagcagg
     5161 taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc gatccgagag
     5221 cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag gttgagcata
     5281 tcgatttagc caacggacat tgccgtcacc ttcttcccac cagcgattct ccaccaaagc
     5341 gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac cactgcttgc
     5401 ctgcgaccag tccaaccagt ttaattggtt atcctgacag taggcattgt tattgccatg
     5461 ctggctgtga ccatgttcgt caccggccag taacatcggc gtgccctggg agagcaacaa
     5521 cgttgttaac agggcatgaa tgctgtcgcg ccgccgttca accaagtcaa gagtaccgcc
     5581 taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc gattttcttc
     5641 tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg taaaaccgtc
     5701 atgcgcggtg acgagattaa tcgcggcact cggcagacga ccattacgtt taaaaacatc
     5761 gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat gtagccagaa
     5821 acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg ggaaatttcc
     5881 cacctgataa ccactaggag cgatatccca cggttcagca attaacttca cctgcgagag
     5941 caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact ctggcgtacg
     6001 gcccatgact gccgccagat caaagcggaa accatcgacg tggcaggttt ctacccaata
     6061 acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga gcgtgttgcc
     6121 gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc tacggttatc
     6181 gatcccacgc agcgagaata acgggccgtc gaggtccagt tccgcactat ggttgagcac
     6241 gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat cgcgaaactc
     6301 atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca tcgccaccgg
     6361 gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac tggcaaactg
     6421 cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt tgatcatcac
     6481 cggatgcccg agggctttat aagtgccacg gatctcgacc gggatctccg ggtgcaagta
     6541 cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg tgcgcggcgg
     6601 ggcgtcatct tcccagtcat agtgatcaac caccactacg catttcggcg caatggcggc
     6661 gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat ctttaaactc
     6721 cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa agcgatgccc
     6781 ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg ggcgcgcatc
     6841 cggcagataa ccgtgccaaa tgtcgccact gttccctggc aagtcatagc gatgttcctg
     6901 gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga aaagtgtgaa
     6961 gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa tggcgagttg
     7021 tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagcgt caggcttagt
     7081 gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc attgcctgca
     7141 ttactgccgt gatagtgtat ggaatcggta ttgaggattt cacgccattt gcctggctga
     7201 ttaatgccga agcgataatc atgccgcggc accggcgtaa agttactggc aacgataatt
     7261 tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt gtcatccacc
     7321 accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt atggtggcgg
     7381 taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt atcgccgcct
     7441 tccaacagat gccagtcgag gctggcgtca tggttccact cgcggccctg ggcaaattcg
     7501 ttccccatga acagcagttt cttgcccgga aatgcccaca tccagccata gtaagcgcgc
     7561 aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga ttttttaccg
     7621 tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt gtagagcatc
     7681 ccgaaggtca gtttatcgtg atgatactga cgataaatcg ggtcgagctt catgtagtcc
     7741 agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc gcccatatcc
     7801 tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac cgcaccggaa
     7861 acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc aagattctcg
     7921 cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc acgataaatc
     7981 atcgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat ccagtaaagc
     8041 gcgttaccga cgaggaagtt actgacttca cggcgaccat agttgtagat cagcgtgttc
     8101 cagtcctgat gatagccttc acgcggatcg ctgtgttcat acaagttcgt gccatcaaat
     8161 tcggcaagcg caaagtcatc agtcgggaag tggcctggca cccagtcgag aatcacgttc
     8221 agaccagctg cgtgtgaggc atcaatgaaa taacggaagt cgtcgcgagt accaaaacgg
     8281 cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa gggatgctcg
     8341 ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg caccagttga
     8401 tcggccagct cgcggtagct caaccagaaa ttgttgtcgg tgtggcgacg ccaggaaccc
     8461 aggtgaactt cataaataga gattggcgca tcaaactgat tcgctttttt gcgctcttca
     8521 gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg gcgcatttgc
     8581 gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc aatcatctcg
     8641 tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccagat gccgctctct
     8701 ttacgcaggc gcatcgggtg acggcgaccg tcccagtagt tgaattgccc aaccaccgag
     8761 acccgacggg cgtttggagc ccagacagag aaacgcgtac ctgtgacgcc atccatagta
     8821 tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga taatagccag
     8881 gcatccattt cctggattag cggaccaaaa cggtaaggat catcaatcag gttttgctga
     8941 ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg aatgacgccg
     9001 ctaaagaatc cccgtgagtc gagacactcc agttttgcga gtttgcgccc ggttttcggt
     9061 tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag tcccgcggtg
     9121 gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat tagcgcgtta
     9181 atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg tgtcacccca
     9241 tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg cgcagcactc
     9301 tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc atgtattcag
     9361 ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga aacatctgcg
     9421 cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta atgaataaag
     9481 attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac agcagctggt
     9541 cgcccacggt aaaggctgac aggaactctg gtcccatatt cagcttacgc aggcggccta
     9601 ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata gtgatttccc
     9661 gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct tcgacggtcg
     9721 gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag cgcaatgccc
     9781 cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg atcttgttgg
     9841 tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt ttgtcgatcc
     9901 acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc tcaccgctac
     9961 gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg gtcgcgagtt
    10021 catctgccac atggccatac agatggccca tctgggttaa taactcacgc atatgtcgcg
    10081 caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca agatcattgg
    10141 cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg ccaacaaaag
    10201 tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg tcaagaatga
    10261 tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa ccttgccatc
    10321 cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga caggtcacaa
    10381 tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg ccagtggttc
    10441 cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca gggcgaatgg
    10501 cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc ataccgcgcc
    10561 agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg tgctgtataa
    10621 atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa agtcgcaagt
    10681 gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt tgttagcacg
    10741 cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat ttctgcagca
    10801 gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat gtccgtactg
    10861 aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct tattgctctc
    10921 ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct cagcctgcga
    10981 gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat taagatgatt
    11041 ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc atttatcgtg
    11101 ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat gttgttgtct
    11161 catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc ctggggcggc
    11221 acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga gaaaggggtg
    11281 aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaactca gatgttcctc
    11341 gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa gagcccggta
    11401 gcgtcacaac taccgggcga acaaacttaa ttcaacagct ggaacgcaat catcccaacg
    11461 atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag cgtttcggct
    11521 tcggtcgcgc cggtaaattt accgaacaac cagaaaccag cgtcgttaac gtggctgaca
    11581 acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc agagtagttc
    11641 agttgttcaa tgactggcat caccagtccc accgccgtta aacaggctac ggtggcagac
    11701 ccctgaatga tgcgcactgc agcagccagc acgaagcagg tgatagcaat cggcaggccc
    11761 atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag cacctgtttg
    11821 aacacgccgc ctgcaccaat caccagtaga atgatccccg ccggttgcag cgcgtgaccg
    11881 caaatgtcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc ataaatcgct
    11941 accagacaag caaccagaat cgcggtaaac ggatgaccaa taaactcgaa ccattcgtaa
    12001 gcggtagagc cttctggcac aaaacgcgcg gcaatggttt tcagccccac cagcaccagc
    12061 ggcaacagga tcagcgacag gctgaatccg aaagatggca ttttaccttc gccgagatgc
    12121 ggttcgctga tgtcgtcagg aatatgcagc tcaacgtaac ggctgatgaa gttaccccac
    12181 agcggcccgg caataatcat tcccggaatt gccgcacaca ggccaatcag gatcatccag
    12241 ccaaagtcgg cattcatctg cgatgccagc agcattggcg ctggcccagg caccaggaac
    12301 gctgccgcgg ccgccacgcc tgcgaataat gggattacca gcttcaccag gttcgtaccg
    12361 gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa caatcgccac ttcaaagaac
    12421 agcggtagcg cacagaccag ccccgcaagg ccgatggcat aatgcgcgcg gctgtgaccg
    12481 aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcata taagatcttg
    12541 ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggtgcc tcccatccct
    12601 ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc agcccccatg
    12661 gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa aaacagcagc
    12721 agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac ttatttgcct
    12781 tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc gatatccacc
    12841 accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg cgtcaccaac
    12901 atttgggttt taaagaaatg gcctttgcgc gctttcaggc gactttcaat cacatcaaaa
    12961 tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa gtcgcgatag
    13021 tgctttttca gtgcagaaca ggcgatcagc gacactttat tggtgcgctg catagcaaac
    13081 gcggagtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag cggttcgcca
    13141 gacgccattt tttcgatatt gcgccgtgga tggaggaaat cgccatcaag aaacgcggca
    13201 tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga tacgcccatc
    13261 aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc acaatacaag
    13321 aattaaaaat attacgggta actgttatcg gtaacattgt ccagccggac aatatcagaa
    13381 gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt gtgggcttaa
    13441 atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt cacagattcg
    13501 ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc acgcggcgtc
    13561 agcacgctcg caagtcgtgg ctccatcacc tgaccaatgt catgaccgtg gaaaccggca
    13621 atcgccatat cgtcaggaac ttttaacccc agacgctgac attcaaacgc cgcgccgacc
    13681 gccagatcat cattagtaca gaacacgcca tccagctgcg gatattcccg ccgcgcctgg
    13741 cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac gctatatggc
    13801 accaggcctg catccagcat cgcctgttcg tatcccttct gtttgatgat agtacgttcg
    13861 tcgagacgtg cgccgagata ggcaatgtgg cgatgcccac gagcaataat ggcggtagtc
    13921 atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca cggcgactgg
    13981 ctgtccatca actccaccac cgggatcccc gccacttcaa tcatctttaa ggtgcgcggc
    14041 gtgtgggtac gttcggtgag gatcaggccg tcgatattcc aggagagcat ggattcgaga
    14101 cgttcttgct ccatttccgg tttataaccg tagtgcgcca gcatggtctg ataaccgtgc
    14161 gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt ggtgagagaa
    14221 ggtaacagga cgccaatcgc ccggctggta gcgttagaga ggatatcggg cgcacgattg
    14281 ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc gacggaaacc
    14341 tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac acggtcagcc
    14401 acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa gagatagatt
    14461 ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct tgtttacaat
    14521 tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc aaaaccccgg
    14581 aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg caggcttcaa
    14641 agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc tgtcgctatc
    14701 cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg tcgaggcttt
    14761 cacgccgtta atggtaacat tgccttttac cacctggatc cagacgcggc gttcagcggc
    14821 aatctgatgc accgactgct catctttcag caacgcccaa cgatacagtt ccatatcctg
    14881 atgcactttc agcgaaccat ctcgcgcatc cggcgagagc accagctgtt tgccctgcac
    14941 ggcatcgaag cgacgctgtt cataacgcgg cgtaatgcca ttttcttcag gcatgatcca
    15001 gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt gacgaatacc
    15061 cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat tgcccatgct
    15121 gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat ctttatgcgg
    15181 gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc gcagcgcgga
    15241 gaagcccata aagttcggat cgtaatagtt agcaaaagag aaagtatgcc aggagtccag
    15301 ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca tttgtattcg
    15361 ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat gatagagggt
    15421 gaaaattgac ccctctgttc aaaaaaattg aactattcga ggggccatca aacttaattc
    15481 gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat ttccagattg
    15541 gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt ttgatacaac
    15601 gcatcataaa cacgcccgta atcgcccatc tccggcttca tctcttctct gaccgtcaca
    15661 ccctcgtcat tgacatactc cagcacaccg accgaatcat ccgctgcgaa tcccggttcg
    15721 cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta tttaataaac
    15781 gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag atggctggtt
    15841 ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc gtcaggattg
    15901 gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc gaacagagaa
    15961 ataatctggt ccatcgtatg cacaccaagg ccatagaacg cgccatcctg cggcagtcca
    16021 ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc gacaatctct
    16081 cccagcttgc cgctttcaat cgcttttttc gccgtcagga agcaggaatc aaagcgacga
    16141 ttctgatatg gcgtgacggt cagccctttg cttttcgcca gtgcaaacag ctctttcgcc
    16201 tgcgcaagtg tcggagtgaa cgttttttcg accagcacat ttttcccggc ttccagcgcg
    16261 cgcttcgcgt actcgaagtg gctgtccgca tgggtgcaga caacaaccaa cttaacatcg
    16321 ggatcgttta gcacttcgtc gagatcgctg gtgaaatgga tatgagagta aatgggagcc
    16381 tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct atccttgcgg
    16441 ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat aaaggcgcag
    16501 ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg caaagcggac
    16561 gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa attgagaacg
    16621 aaagatcaaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc aatgtgagca
    16681 atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat aataatcatt
    16741 ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc atttgaaagt
    16801 caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg agatagtaat
    16861 acgccacgca gaaacacggg attacgagac catcaggcag attcacgccc agccggaggt
    16921 gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg agcgactcgc
    16981 cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg tgggccatct
    17041 caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg gtatctgtgt
    17101 cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga tgattgaaat
    17161 gtgcgacaac tggttacggg tagatcgcat tgaactaacc gtgtttgtcg ataacgcgcc
    17221 agcaattaag gtctataaaa aattcggctt tgagattgaa gggaccggta agaagtacgc
    17281 attgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat agagaatttg
    17341 caatctaagt ttgtcattta agccctcacc taccgggagg gcttaagata ttaatacccc
    17401 gccgttaaat catccaccga gcgcgggtcg gatgcgccgt acaactcacc gtccggccca
    17461 accataatgc tttgtgtgct gcccatcgcc tctttcagcg ccactttctg accttttgct
    17521 tccagcagct tgagcgtatc cgggctaaac cctttttcga cacgcagctc gtccggcaac
    17581 cactgatggt ggaaacgcgg cgcattggtc gcttcggcga cgttcatgcc ataatcgatg
    17641 ctattcacca ccatttgcag caccgtggtg ataatccggc taccgcctgg gctaccggta
    17701 accagccagg ttttaccgtc tttcaccaca atggtcggcg acatcgacga cagcgggcgt
    17761 ttgttcggcc cgacggcgtt ggcatcaccg cccaccagcc cgtaaacgtt cggtacgccc
    17821 ggtttggcgg agaaatcatc catctggtta ttaagcagaa taccgctctc gcccgcgaca
    17881 atacccgtac cgaaggtggt gttcagcgta taggtcaccg ccaccgcgtt accgtcttta
    17941 tccactacag agtaatgggt agtttgatta ctctcataag gcgcaagctt gccagggcga
    18001 atctcactgg acggcttcgc ttttttgata tcaatttgat cggcaatgga tttggcatag
    18061 gctttattgg tcagtgcctg ccacgggact ttgacaaaat ccgggtcgcc aagatattcc
    18121 gagcggtcgg cgtaggcgta tttctccgct tctgccatga tttgcatcgc atcggcgctg
    18181 ccaaagccgt atttcttcat atcgaagttt tccagaatat tgaggatttg tacgatatgg
    18241 atcccgccgg aggatggcgg tggcatggag taaacctgat acccgcgata atcgccgctt
    18301 atcggagtgc gttcgaccgc tttataggcc gctaaatctt ctttagtgat caagccaccg
    18361 tttttctgca tctcctgggc gatctgttcc gcaatcgtgc ctttatagaa ttcgtccggg
    18421 ccgttttcag caatcatctc caggctcttt gccaggttcg cctgcaccag cgtgccgccc
    18481 tttttcagcg gctcgccctc tttccagaag atagctttac tgttttcgtg attcggcaac
    18541 acttcgctac cgtaggtttt gagatcgtca gccagcgcgt cgttaacgat aaaaccatcg
    18601 cgtgccagtt taaacgcggg ctgcacgact ttgttcagcg gcatggtgcc gtatttatcc
    18661 agcgccagcg agaaacctgc taccgtaccc ggtgtgccgg aagccagatg cgaagtgagt
    18721 gattttttgc tgtccgggtt gccctgatca tcgaggaaca tatcgcgggt cgctttggcg
    18781 ggtgccattt cgcggaaatc gatagccgtg gtattgccat ttttcgagcg gattaacata
    18841 aaaccaccac cgcccagatt ccctgcctgc ggatgcgtta ccgccagcgc gtagcccacc
    18901 gccacggcgg catcaacggc attcccgccc tccttgagga tatccacccc cacctgagtg
    18961 gcagtggcgt ccacagacgc taccattccc tgtttcgcgc gtaccggatg gaagacatct
    19021 tcctccacac catacgagac gggcggcgct aaaacaactt cctgagagca gagcagcaat
    19081 ggccacccgg cgtaaaaacg tcggttttat catcgttatt ctccagagat taaggggcaa
    19141 ccccagctaa gcctggtata taactctgaa ttaatcatcg ttttgtcggg aagcgagtaa
    19201 acttaaagga tatcttcaga ggaggatacg cgatgaaacg acttctgatt cttacggcac
    19261 tcctgccgtt tgtcggcttt gcacagccca ttaatactct gaacaaccct aaccagccgg
    19321 ggtatcagat acccagccag cagcggatgc aaacccagat gcagactcag caaatccagc
    19381 aaaaagggat gctgaatcag caactgaaaa cgcaaactca gttgcaacag cagcatttag
    19441 aaaaccagat aaacaataat tctcagcggg tgttgcagtc acagccgggg gagcgaaatc
    19501 ccgcccggca gcaaatgctg cccaacacca acggcgggat gttaaacagc aaccgtaatc
    19561 cggatagttc gttgaatcag cagcatatgc tgccggagag gagaaacggc gacatgctga
    19621 atcagcccag cacgccgcag cctgacattc cgttgaaaac tattgggccg taaagttcgg
    19681 accaatcacg tcaatcgcat cggtacagat gcaatccacg ccccagcgca gcaactctgc
    19741 cgcgcgctgg ggtttattga cggtataaac cagaatccgt aatccggcgt ctttcaactg
    19801 catcactcgc gctttatcga gtaacttatg attgagatga atagagacgc agcccagccg
    19861 cgcggtcagt tcgcgccagt cgtcgcgcca ctcatcaagc aataaaccac gcggcagttc
    19921 cggtgccgcc tgttgtgcag cttctaaagc atcaatctca aacgatgaca gcagcggcgg
    19981 cgtcataccg gcccacagtt ggcgtgccgc cagcgccacc attttgcccg ttaatggtcc
    20041 agtgccagtg gtgggtttga tttcgatatt cgccatcatc ccgtgttcgc gacagcgttc
    20101 cgccacctgc gaaagcaacg gcagcggctc acctttaaac gctttgctgt accaactgcc
    20161 cgcatccacg cgcagtaaat cctgccagtt cagttcaccc gcgacgcccc agccgttgct
    20221 ggtgcgttcg agattgtcgt catggagcag gaagatctcg ccatctttcg ataacttcgc
    20281 gtcaaattcg atcatcttat gaccgtattt tgccccgacg tcgattgccg ccagggtgtt
    20341 ttccggggcc agcttaccgc cgccacgatg agcgacgatg cggggataag gccagttact
    20401 catactcgtt gtcctgtttc accatcaaaa agatgcagct gattttccgc cagatgcagc
    20461 cacagcgtgc tgcctgccgt cgggcgctcc tgatgcgcca gtcgcaccac cagcttctgc
    20521 ttgcgagctt agcgcaatat gttccgggcg aatgccgagg gtcattttac gcccggcata
    20581 ctgacggtag ccaccgttta gcggtagcac gataccgccg tccagttcga aatgcgtgcc
    20641 ttcattattc atgcggcctg tcagcaggtt catcgccggg ctgccgataa aactcgccac
    20701 aaacaggctg gcgggctttt cgtagacttc caccggcgtg ccaatctgtt cggcaacgcc
    20761 gccgttcatc accattactc gctgggcgag cgtcatcgcc tcaacctgat cgtgagtaac
    20821 gtagagtgaa gtcgttttca ggcgacggtg cagctgttgc agttcaagac gcatctgtac
    20881 gcgcagcttg gcatcgaggt tagagagtgg ctcatcaaac aagaacaccg ccggatcacg
    20941 cacaatcgcg cgtcccatcg ccacacgctg gcgctgaccg ccggaaagct cgcgcgggcg
    21001 acgtttgagc agaccgtcca gctccagaat gcgcgccgct tctttaacgc gctcggctat
    21061 ttgctgcttg cccatgccgc gaattttcag cccccacgcc atgttttctt cgacgctcat
    21121 atgcggataa agcgcgtaat tctggaacac catcgcaatc ccgcgatctt tcggctccat
    21181 ttcggtcacg cgctggtcgt tgatccagat atcgccttcg gtcaccctct ccagcccggc
    21241 aaccatacgc agcagcgtcg atttcccgca gccagacggc ccgaccatca cgataaattc
    21301 gccatccgcc acatcaaggg tcagcggttt aatgacctag gttttgccat cccagctttt
    21361 ggttactgcc tgtaatttca gtcctgccat cttatttctc actatcgacc aggccgcgca
    21421 cgaaggcacg ctgcatcact aaaacaatca ctactggtgg gatcagggtt aacaacatcg
    21481 ccgccatcac tgagttccat tccgtggtgc cttcgccagt ggcgatcatc cctttgatcc
    21541 ctgccacggt ggtgccgaga tccacgtcgg taataatcaa caacggccac aaatactggt
    21601 tccagccgta aataaaggtg atcacaaaca gcgccgccag attagttttg gagagcggaa
    21661 aaacgatgtc gcaaaagaag cgcattggtg acgcaccgtc gatccgcgcc gcttccacca
    21721 gttcatccgg cagcgtcata aagaactggc gaaacagaaa cgtggcggtc gccgaggcca
    21781 tcagcggcag cgttaaaccg gcgtagctgt cgagcatctt caggttggcg atgacttcca
    21841 ccgtcgggaa gatacgtact tcaaccggca gcatcagggt gataaaaatc atccagaaga
    21901 agaggttacg tagcggaaaa cgaaaccaga caatggcaaa tgccgagagc atcgagacag
    21961 taattttgcc gagcgtaatg ctgaacgcca tcacaaagct gttaagcaac atccgccaga
    22021 acggcgcgct atttgtgcct accccgttca cccagatgtt gtggatgttt tccagcagat
    22081 gcgtgccggg gatgagcgtc atcggcgcgg cgtagacggc ctgtttatcc agcgtcgccg
    22141 cgacaaacgc cacgtacagc gggaagagga tcaccgcgat cccgaggatc agcatggtat
    22201 ggctgaatat cgtcagccac ggacggttct caattattgg taacgcacct tgctttcaac
    22261 atagcggaac tgcaccaccg tcagcacgat gacgaggaac atcaacacca ccgactgtgc
    22321 ggcagacgaa gccagatcca gtccggtaaa accttcgcgg tagatcttat aaatcagcgt
    22381 cgtggtggcc tgaaccggcc cgccggacgt ggcggcgtcg atcaccggga aggtgtcgaa
    22441 gaaggcatac accaggttca ctaccagcag gaagaaactc accggggcga taagcggcag
    22501 cgcaatctta aagaagcggc gaatcggccc tgcaccgtcg atggctgcgg cttcgatcaa
    22561 cgaacgggga atggattgca gcgcggcata gaagaacaga aagttgtagc tgatttgctt
    22621 ccacaccgag gcaaacacca ccagaaacat cgcctgaccg ctattttgcg cgtggttcca
    22681 gtcgtagccg aactcagcga gaaaatgggt gatcagcccg cgaccggggt taaacaggaa
    22741 gatccacaat acggcggcaa cggcgggagc cacggcatac ggcagcaaca ttaaggtttg
    22801 gtagaaacga ctgccgcgca cgatgtactc caccagcgcc gcaaagaaca gcgataccag
    22861 caaaccgctg acggtgacaa aggtgctgaa ttttatcgtc gtccagaagg agtcgagata
    22921 atagctgtca tgaaacagcg tgacgaagtt atccagaccg acaaactggc tggagaaacc
    22981 aaacggatcg acgctttgta gcgagtacca caacgcttcg cccgcaggcc agataaaaaa
    23041 gataacggtg atgatgagct gcggcgcgac cagcagataa ggcagccagc gcgagcggaa
    23101 caccggacgg gatgatgaca ttacggttaa ttcctgaact gtgccggatg cgcttcgctt
    23161 atccggccta cacgcgatgc caggtcagat aagcgaaacg catccggcat tacactgatt
    23221 aagacttcgt cgatttctca aagcggcgca gtaactggtt cccacgctca actgcggtat
    23281 ccagcgcctg ctgtggcgtc ttcttaccgg tccacacgct ctcaagctct tcatccacaa
    23341 tcacgcggat ctgcggcatg ttgcccagac gaagcccttt ggtgaacggc aacggcggct
    23401 tgttcagcat ctgacgcgtc gcggtatccg cacctgggtt tttctcgtaa aagccctgct
    23461 cacgggtcag gtcatacgcc gctttggtaa ttggcagata accggttttc tgatgccact
    23521 cagcagcgtt ttctggcttc gcgaggaagt cgagaaactt cgccacgccg gtatacgttt
    23581 ctttatcttt gccctgcatc acccacaggc tggctccgcc gataatggcg ttttgtggcg
    23641 catctttcgc atcggcgtcg taaggcatca tacctacgcc gtagttaaat ttggcgtact
    23701 cgcgaatgtt ggcaagagaa ccggaagagg cggtggtcat cgcgcaatca ccgttataga
    23761 acttctcggt ggattcatcc ttacgaccga cgtagctgaa gtcgcccttc ttgttcatct
    23821 cctcgagcat ggcgatgtgt ttcacctgct ccggcttgtt gaactccagc accgcgtccg
    23881 tgccgtcaaa gccgttgttt ttgctggcaa acggcagacc gttccaggcg ctaaagtttt
    23941 ccagttggat ccagccctgc cagccgctgg cgtagccgca cttaataccg gaggctttca
    24001 gttttgcgga atagtccgcc agatcctgcc aggttttcgg cggctgttcc gggtctaacc
    24061 ccgctttctt gaaggcgtct ttgttgtaat agagaacggg agtcgagctg ttgaacggct
    24121 gggagagtaa gtggccggtt ttgctgtcgg agtagtaacc ggaaaccgtc ggcacaaact
    24181 gcgactcatc gaactgaatc cctgcctctt taaacacgtc atacaccggt ttaatggctt
    24241 tcgacgccat catggtggcg gtgccaactt cataaacctg caaaatagcc ggagcgttgc
    24301 cggtacgaaa tgcggcaatc cccgcgctta aattctgttc gtagttgcct ttataggtcg
    24361 gtacaatttt gtaatccgga ttttcggcgt taaaacgttg ggccagagaa tccacctctt
    24421 tacccagttc cccttccata gaatgccaga acggaatggt cgtcactgcc tgtgcattcc
    24481 ccattaacgc cagtccgagc gccagtgctg aagctgtata atgtaacggt ttcatcgttt
    24541 atctctcttg ttgtaccgaa tgcgcgaatt cacgcgtttt atgctcgcgg ggtaacatga
    24601 catgctcgaa ttacagaaaa ataacttttt tgttacattt gtaagatagt aaggtgtcag
    24661 aaagatgaca aggcggtgac ggcgtgggtg agggaaaatg ggagatgggg cacggataag
    24721 cggaaaaata gaaggtctga atcaaactct acagattact catcgtttca tgccggatgc
    24781 ggcgtaaacg ccttatccgg cctacaagat cgtgcaaatt caatatattg caactcaccc
    24841 agtaggcctg ataagcgcag cgcatcaggc aattttgcat ttgtcacctg tctcaaagga
    24901 gtcttttgac tccttatcaa tcaacgtgtt attacccgcc taaatacgaa cttctcactg
    24961 cttcgttcgc cagcagcgcg tcgccggtat cggagagcac cacatggccg ttttccagca
    25021 cgtagccgcg atccgccagc tttagcgcct ggttggcgtt ctgctcgacg aggaagatag
    25081 tcatcccctg ctcgcgcagc tgctcgatgg tgtcgaaaat ttgctggata ataatcggcg
    25141 caagaccgag cgatggctca tcaagtaaca acaaacgcgg attgctcatc agcgcgcgac
    25201 caatcgccag catctgctgt tcaccgccgg acatggtgcc cgcccgctga acacggcgct
    25261 catgcagacg tggaaacagt tcatacaccc actttatgcg ctcctgaaac tggtcacgtt
    25321 cggcaaagaa gccgcccatc gccaggttct cttccaccgt catccgcgag aagacgcgac
    25381 gcccttccgg gacaatcgct accgcttcgc gcatgatttt cgccgtctgc cagtcggtaa
    25441 tgtctttatc atcaaacaca attcgcccgc tggtggcgcg cggatcgccg cataacgtgc
    25501 cgagcaaggt ggttttcccc gccccgttcg cgccaatgag agtgacaatc tcgccctgat
    25561 tgatatgcag gctcacctca tgcagcgcct ggattttgcc gtagtgggcg ctgactttgt
    25621 caaaggacaa catgactttt tccatcttat gcctcaccta aataggcacg gatcacgtcc
    25681 gggttattac ggatttgctc cggcgtaccg tttgccagtg gcgtcccctg attgaccaca
    25741 taaattcggt ctgaaatccc catcaccagc ttcatatcgt gttcaatcaa caagatagtg
    25801 gtgttgtgat gattgcgcag ttcggcaatc agctcatcca gctctttggt ttcttttggg
    25861 ttaagacctg ccgccggttc gtcgagcatt aaaatctccg gctgcgtcac catgcagcgg
    25921 gcaatctcca gacggcgttg gtcaccatag gccaggttac ttgcctgacg gttggcgtgt
    25981 tccagcaaac caatgcgctc aagccaggtg gcggcgcggt cgagcgcttc gctctgggcg
    26041 cgacggaaag aaggcgtttt caacaggcca gagaacagcc cggttttcag ttgctgatgc
    26101 tgcgccacca gcaggttttc aattaccgtc atttcacgga acagacgcac atgctggaag
    26161 gtgcgcacca cgcccatgcg agcaatttgc tgccccggta aaccttcaag atgctgatcg
    26221 cgcagtaaaa tggtgccgcc ggtgggtttg tagaaaccgg tcaggcagtt aaaaaccgtg
    26281 gtttttccgg caccgttagg gccgattaac gagacgattt cctgcgggta cagttcaaga
    26341 ttgacgttgt tcaccgccag caggccgccg aagcgcatca tcagaccgtt aacagataat
    26401 aatggctgac tcatgcctgc tctcctttcg ctgcgccgtt tttcagcttc agttgcgggc
    26461 gcgtcatggg cagcaaaccc tgcggacgcc agatcatcat cagcaccatc aaaccaccga
    26521 gcattaacat gctgtattcg ttgaaatcac gcatcaattc gcgcgacacc accagcagaa
    26581 cagccgccag aatcactgca aattgcgagc ccataccgcc gagcaccact atcgccagca
    26641 caaaggccga ttcagcaaag gtgaaggatt ccgggctgac aaagccctga cgcgccgcaa
    26701 acagcgttcc ggcaaaaccg gcaaatgcgg cgctgatggt aaaagcggtc agcttgatac
    26761 ggcgcgggct taagcccagc gaacgacagg cgatctcgtc ttcacgcaac gcttcccacg
    26821 cgcggcccag cggcatccgc agcaggcggt taatgacaaa caggcttaac accaccagca
    26881 gcaacgccac caggtagagg aagataacgc gatcggacgg atcgtatttc aggccaaaga
    26941 aattactgaa cgtgtcccag ccgccttcac gagcggtacg gctgaactcg agtccgaaga
    27001 gtgtcggttt cgggatctga ctgattccgt tcgggccgcc ggtgatttca gtgttattga
    27061 gcagcaatat gcgcacaatt tcgccgaaac caagggtaac gatcgccaga tagtcaccgc
    27121 gcagacgcag caccggaaaa ccgagcagga aacccgccgc cgctgccatc agtccggcaa
    27181 tcggcaaaca ggtccagaac cccagaccgt aatagtgatt gagcagcgca aaagtgtaag
    27241 cgccgatggc gtaaaaaccg ccgtacccca gcaccagcag accagaaaga ccaacaacca
    27301 cattcagccc gagaccgagg ataatgtaga tcatggtcag agtggcgata tccaccgtcc
    27361 cacgtgacac cataaacggc cacgccacca caagcaccag cagcgccacg aggaacagct
    27421 tttgcttcac cgtggaccca tcaatcgcgg gcagaataaa cttcggcccg gaaacgcttt
    27481 tcaacccttt ctggaaagcc ggtcgcaaaa gctggaagaa aaagaccacc gccgtgccga
    27541 taaacatcca ctgccaacgg atgtctgaag ccgtgtcgac caccagtttg gtgccatcca
    27601 gctccagttg tacgcccata aagacgcccg ccaggacaaa gaacatcgcg gcagagagca
    27661 gtgccattgc aatatgcatc ggtttcatac tttctctacc tccgggcgac ccagaatacc
    27721 ggtcggcatc accagcagca ccagaatcag cagggcgaat gagaccacat ctttatattc
    27781 cgtactcaga taggcagaag agagcgcctc cgcaatccct aggatcagcc cgccaatcat
    27841 cgcccctgga atgctgccaa tcccaccgag caccgcagcg gtaaaggctt tcatcccggc
    27901 cataaagccg atataggggt taatgacgca gtagaactga ccgagcagta cgccagccac
    27961 tgccgccatc gccgcgccaa taacaaaggt caaagcaatc acccggtcgg tgttgatgcc
    28021 aagcaggctc gccattttca gatcttccgc gcaggcacga caggcgcgcc ccatacggga
    28081 atagcgaatg aaaatcgtca gcgccagcat ggcgaggaag gtgacaatcc agatcaccgc
    28141 ctgcatggtg gtaatagagg cagagaagtt ttcgctatgg cccaccaccc actgaccgtt
    28201 aaacaggctc ggcagcgcca cgtcgcgcga accttcggtc aggctgacgt agttttgcag
    28261 gaagatggac ataccgattg cggaaataag cgcaatcagg cgcttagagt tacgcaccgg
    28321 gcggtaagct acccgttcga tactccagcc gtaggcgctg gcgatgacga ttgcgccgac
    28381 gaatcccgcc gccaccagca gccagccggt atcaatgccc atcatcatca gcgcggcgat
    28441 gatcataaat gagacgtagc tgccaatcat ataaacctcg ccgtgggcga agttgatcat
    28501 gccgataatg ccgtaaacca tggtgtagcc gatggctatc agcgcgtagg tactgcccag
    28561 cgtgacgccg ttaaacatct gctgcaagaa atacaaaaac tgctcagaca taaggtaacc
    28621 tttctaaacc cgcccgcatt ttacgggcgg tgggatgatc acttggctgc cgtggatgaa
    28681 ccgtcggcgt gccactggaa gacaccaaaa tcaaatccct taagatcgcc tttttcatcc
    28741 cagttcagcg gcccaatcac ggtgtttgca ccgttagctt ttaaatcttt caccagcgcc
    28801 agcggctcat cgctgccggt acgctcaagg gcagttgcca gagattgcac tgccgcgtaa
    28861 gtgatccaga cataaggccc ggacggatct ttcttgtctg ctttcagcgc atcaacgatg
    28921 ccctggtttg ccggatcctg gtcatagcgt tttggcatag tgaccaacat gccttcggcg
    28981 gcatcaccgg caatgttcga cagcgatgcg ttaccgacac cttctggccc cataaactgg
    29041 gttttcaggc caacggaacg ggcttggcgc agcatctgcc ccatttccgg gtagtaaccg
    29101 ccgtagtaaa cgaagtcgat attttctttt ttcaggcggg cgatcagcgc ggagaaatct
    29161 ttctccccgg cggtaatacc gtcgaagaag acgacgttgg cgttagccgc tttcagcccg
    29221 tcctgcaccg aacgcgccag cccttcgcca tactgttgtt tgtcgtgaat gatggcgatg
    29281 cgctggggct tcaccgtctc aagaatgtat tttgccgccg taggcccctg ggaagagtcc
    29341 agtccggcag tacgcataat gtgttgataa ccgcgttggg tcagctccgg gttggtcgct
    29401 cccggcgaga tcatcagaat accttcgtct tcgtagatat ccgacgctgg ctgggtagag
    29461 gaagaacaca gatgaccaat aacgtattta atgccgtcgt taacgatttt gttggcgacc
    29521 gcaacggctt gtttcgggtc gcaggcgtcg tcatattcca cgccaaccag cttgtcgccc
    29581 ttaattcccc ctttgacatt aatgtcttta atcgcctgac gcgcgccgtt aaattccata
    29641 tcgccccact gggcaatcgg gccggacatc gcgccgacaa cggcgacttt aatatcgtca
    29701 gccatagcgg tgtgtgaaat tgccagtgca atcatccctg cgatgatagt tttcgcattc
    29761 cgtttcatag tcaaaaatcc ccattcgtga tgttgtgttg ctttgttttt atgtgttaac
    29821 aaatcagact gttctttttt tatactgcac tgtttttgcc tgtctgatta aggggttagc
    29881 gcagtatttt gtgatgatag cgattaaaat ccctattttt cagtcgatta agaacagata
    29941 atattctgaa tttattgata gataaacaga aaaaagtgcc tttgtcagca taaaataacg
    30001 gcacaaaggg cggaataatt cactatcatt caggggatta tgctggacat ttttcattct
    30061 ctaatgtttt aactttgtaa ttattgctgt taaaaaatta atcacctgcc aaaagaaata
    30121 aaaaagagaa agcctccgat taaattattt cgctacactg gttccacttt tgtgatttac
    30181 acgggttacc catgaagctg accatcattc gattagaaaa ctttagcgac caggaccgga
    30241 ttgacctgca aaagatctgg ccggagtatt ccccttcctc tttacaggtt gacgataacc
    30301 accgtatcta cgccgcgcgt tttaacgagc gtctgcttgc ggccgtgcgg gtaaccttaa
    30361 gcggcaccga gggagcactg gattctctac gcgtgcggga agtcacccgc cgtcgcggtg
    30421 tggggcaata tctgctggaa gaggttttgc gtaacaatcc tggcgtttca tgctggtgga
    30481 tggcggatgc aggcgtggaa gatcgcggtg tgatgacggc gtttatgcag gcactgggat
    30541 ttacggcaca gcagggcggc tgggagaagc gttaatcgtc aagttgattt caaaagtgat
    30601 gaccaacgag aaattgcctg atgcgctacg cttatcaggc ctataatgtg tgctgcaatt
    30661 tactgatttc tttggttctt gtaggccgga taaggcgttt acgccgcatc cggcatgaag
    30721 caacgtattc gatattagca atttggcggc aacccaaagt tgccgattaa caattacttc
    30781 gcatcggtcg ccgtgccgtt ggcgtgccag tcaaatacgc cgaactcaaa gccctttaga
    30841 tcgcctttct catcccaggt cagtggcccc atcacggtat ccacggagtt cgcttttagg
    30901 tatttggcga tttcagccgg atcgtcagac tgattcaggc ccgcctgcaa agattgcagc
    30961 gcggcgtagg tggtccagac gaatgcgccg cttgggtcct gttttttcgc tttgatcgca
    31021 tcaacaatgg gtttgttcgc cggaacctga tcgtagttct tcggcttggt caccagcagc
    31081 ccttccgctg attcgcccgc aatgttagac agcgaaacgt tagccacacc ttccggcccc
    31141 ataaactgag ttttcagccc tgccgcgcgt gcctgacgca ggatttgccc catttccggg
    31201 tgataaccgc cgtagtaaac gaagtcgata ttctcttttt tcagacgcgc caccagcgtt
    31261 gagaaatctt tttccccggc ggtgatacca tcaaagaaca ccacgttagc attgcctttc
    31321 ttcaggccgt cctgcaccgc tcgtgccaga ccttcgccgt attgctgttt gtcgtgaacg
    31381 atagcaatac gctgcggttt cactttctca agaatatatt tcgccgccgt cggcccctgg
    31441 tcggagtcca ggccggtggt gcgcaggatc agctgatagc cacgggcggt cagctccggc
    31501 gcggttgccg ctggggtgat cattaaaatg ccttcgtctt cgtagatgtc agacgcaggc
    31561 tgcgttgatg aagaacagag gtgaccaatc acatatttaa taccgtcgtt aacgactttg
    31621 ttcgccaccg caaccgcctg tttcgggtca caggcatcgt catattttac gatttgcagt
    31681 ttgttgcctt taatgccgcc tttagcgttg atatccgcaa ccgcctgctc tgcgccggta
    31741 aactcctggt caccgtactg cgcaaccgga ccggacattg cacccacgac cgcgacttta
    31801 atatcttctg ccagagccat attgctgaat gccagcgcga tacatcctgc cagtaacgct
    31861 ttacccttta tgttcatcct gagaatcccc attcttctgg ttattacgtg tgttgtgatg
    31921 ttgttgttca gcactttatt tcgttttatg catgactacc cgtgctttag cagcatactc
    31981 tgctaaaaca tacccgattt ttatgatatt ggaatagcta ttttgacagt ttattaacaa
    32041 tctgcgtggg gattggcgtt ttgccggagg gggaattgat tattgtagag gcccaaaaaa
    32101 caaaaacccc ggactctcat ccagggttct ctgcttaata gcggaaatta cgcttcaata
    32161 gcggcgcgaa gttttttcat cgcgttcttt tccagttggc gcacacgttc agcggaaaca
    32221 ccgtaacggt cagccagttc ctgcaacgtg gacttgttgt cttcgtccag ccagcgcgca
    32281 cggatgatgt cctggctgcg ttcgtccaga ccatgcatcg cgtcggtcag acggtttgcc
    32341 gcctgctctt cccagttatc atcttcgatg ccgtcggcaa agttagatga tttatcctgc
    32401 agatagagca ccggtgccat cggctggctg tcggaatcgt cgtcggaaga caggtcaaag
    32461 gtcatgtcct gtgccgccat acgtgattcc atctcacgta cgtctttgct ggttacgccc
    32521 agttcacggg ccaccatttc gacttcatcc tggttaaacc agcccagacg ctgcttggtt
    32581 ttacgcaggt tgaagaacag tttgcgctgc gctttggtgg tcgcaacttt gacgatacgc
    32641 cagttacgca gaacgtattc gtggatctct gctttgatcc agtggacggc gaaggagacc
    32701 aggcgcacac ccacttccgg gttaaaacgg cgcactgctt tcatcaggcc gatgttacct
    32761 tcctgaatca aatccgcctg tggcaggcca tagcccgcat aattacgagc aatatgaaca
    32821 acaaaccgca ggtgagacag gatcagcgtt ttagctgctt ccagatcgcc atggtaatgc
    32881 agcttttcag ccagcgcccg ctcctcgtca gccgacaaca tcggccacgc gttagctgcc
    32941 cggatgtagg aatccaggtt gccaactggg gctaaagcta aactttgcat tttgtcagtc
    33001 attcaaatcc tctcaatcga tatcttctgg cgcttcagtg gtagcaacaa ctgtgccaaa
    33061 gcttaagagc aacgaggtta tcattcactg ttttatcaga ccgtgatttt atccacaagt
    33121 tcaatgcaag cttgtgaata aattacgcac aaaatgtgac atagagatga aataccggga
    33181 agagacaacg gggtctcttt ccctgctacg gaacccattg cagggaaaga gtataacacg
    33241 cttttattat tcaggcgtaa agtggcgtaa atgttgtacc gtggcaagcc acgctgccac
    33301 ccagccaatc atcgagcata ccagcagcaa tagcaggcat tcatcgaacg ataaaccatt
    33361 gatatcaaac ttcgttccga aaacctgtgc cacttccgcc accgctgacg acaaacgcag
    33421 taccagaatt tctgacaaaa ttaatgacaa caatgcgcca gaaaatccca gcagcgcacc
    33481 accgtacagg aacgggcgaa ggatgaaacc atccgtcgca ccaatcagtt tctgcacgtt
    33541 gatggagtca cggcgagcaa agatgctcag acgtacgctg ttgccgatga cgaggaacac
    33601 tgccgccacc atcaacacgc cgatcatcgc cgaaacgcgc ccaaccagcc cggtcaacgc
    33661 cgccagacgg gcaaaccagc tgtcatccat ccgcacttcg tcaatgccgt taatctgcgt
    33721 gatacgatca cgcagcgtat tgagtgattc cgtcccctga aaatcgagtt tcgggatcac
    33781 caccgccact gccggaagcg ggttttcttc cagcatatcc agcgcaccac caaaaccaga
    33841 ccagttacgg aactcgccca gtgcgtcttc acgagaaaga tagttcactt tctccacgcc
    33901 ttgctcggcc tgcaactgtg ccaccacgcc cgcagcggcg tcatcgtcca gcgttttttg
    33961 caagtaaaca gtgatttgcg gtgacggata atactgcgtc gccgcctgat taacgttttt
    34021 gtacaccata taacagacgc tgggcagcgt cagggagatg gcgataacca tcaccgttaa
    34081 aaacgtggcg aacggtttgc ttttcagatc ctgcaatgcg ccgtggaagg catagcgcac
    34141 ctgttcgttg aaaacgttgg ttttgcgatt taccggtttt ggcgaggatt tcgcgcgttt
    34201 cggtgcgtta cgaccgccgt cgcctgagcc gccgaccgat ttacggaagc gatccagacg
    34261 cccaccaaac tgccgaatat gattgattgc atcgcgctta ttcatggccc acgcctccat
    34321 gcaagtgacc atcgctcagg gtgagcatgc gataggaacg ccgtgaaatc aggttaatgt
    34381 cgtgcgttgc catcaatacg gttaccccaa cgcggttaaa ttcttcaaac agacgtagaa
    34441 tcccttccga caacgcgtcg tccaggttac cggtcggttc gtccgccagc agtaccgcgg
    34501 gcttattcac caccgcgcgg gcaatgccaa cacgctgttg ttcaccgccg gaaagctgaa
    34561 tagggaagtt cttcgctttg tccagtagcc cgactttatc cagtgccgcc gacacccggc
    34621 gacgaatatc gtcaccgctg gcaccagcga taatcagcgg gatcgccacg ttatcgtaga
    34681 cagtacggtc catcagtaga tggtgatcct ggaaaatcat gccaatctgg cggcgcagaa
    34741 acggaacttc acggtttttc agacgcgtga tgtcatggcc gctaaaccag attttcccgg
    34801 cgctgggccg ctcaatccca cagatcagct tcaggagggt acttttccct gcgccggaat
    34861 gaccggtcag aaacgccatc tcacccggct gcatatggaa cgtaacgccc tgcagcgcct
    34921 gtctcccacc gagataagcc ttgctgacat gttcaaagcg aatcattgtt aatcctctcg
    34981 ggcaaaaagt gcctctataa agtcgtccgc cttaaacgga cgcaaatcct caatacgttc
    35041 gccgacacca atgtagcgga tagggatacc aaactggtca gccaccgaga aaattacccc
    35101 gcctttcgcc gtgccgtcca gtttcgttag cgtgatgccg gttaagccaa cagcttcgtg
    35161 gaacagtttg gcctggctta ccgcgttctg cccggtgctg gcatcaatag tcagcataac
    35221 ttcatgcggc gcttcaacgt cgagtttctt catcacgcgg acgattttct tcaactcttc
    35281 catcagatgc gatttgttct gcagacgtcc ggcggtatcg gcaatcagga cgtcgatatt
    35341 acgcgcttta gctgcctgaa tggcgtcgaa gataacagag gcggaatccg caccggtatg
    35401 ctgggcaatc accggaatat tgttgcgctg accccagacc tgaagctgtt caaccgcagc
    35461 tgcacggaaa gtatcgcccg ccgccagcat caccgattta ccctgctgct caaactgacg
    35521 cgccagctta ccaatcgtcg tggttttacc cacaccgttg acgcccacca tcaggatcac
    35581 gaacggcgtt ttatcttcaa cattcagcgg ctcatcgact ttcgccagaa tctcacccat
    35641 ctcttctttc agcaggccat agagcgcctc ggcatcacga agctgcttgc gggatgcgcc
    35701 ttccgtcaga ttggtgataa ttttacgtgt ggtttccacg cccacatcgg cgatcaacag
    35761 ctgttcttcc agctcttcaa acagatcatc gtcgattttt ttaccgcgga acaggctgat
    35821 aaatccggaa ccgagatttt ctttggtttt taacaggctg cgtttcaggc gcgcgaagaa
    35881 gccttctttg gtcggttttt cttgctcctg ggcaatttct tcaaccggct gttcttcttc
    35941 tgccggagga actaccatca ccgcctcttc tgccgcttcg gcagccagcg cctgtgcttc
    36001 cagctcttcg tcggtgattt cttctttagc cgcttcttct tctgccgctt cgacaatctc
    36061 tacggtttcc gcttcagcct gccactcttc tggcgaaacc tcttcggcgt tgacgtcttc
    36121 cggcaacggc agctcttcac gttcgatagc cactggctcc ggcgtttctt ctacgaccgg
    36181 ttccggctgt gcaacaactt ccgcttcagg ctgcgctttt tcactttcaa caacctgttc
    36241 agtgacttcc acaacgtcgg cagcaaaagt ttccgcctcg gcttcagtat gcgcctgcgg
    36301 ctgctcttca acggcttgtt cagaagcctt cgcaggctct tgcgcctgaa cgatttcttc
    36361 tacaaccggt tgttcattct gaacttctgt ctctttttcc ggggtctgct ctttttgacc
    36421 aaagcccagc caggaaaaaa agccacgtct tttttctttc gccatttgcg actacactcc
    36481 tcgctgttgc ttcatggcac agcgttaacg ctatgtacat ggcagctaaa aaaatgatga
    36541 aatagtctat cacttaactt aattcacatc accgcctgca agtatatgtt atctggcgga
    36601 ttgagcaatt tatcatgaaa aaaccgaatc attccggcag cggccaaatc cgcattattg
    36661 gcgggcagtg gcgaggccgt aaactcccgg ttcctgatag cccaggtctg cgccccacca
    36721 ccgaccgcgt acgcgaaacg ttgtttaact ggctggctcc ggtcattgtt gacgcccaat
    36781 gtctggattg cttcgccggg agcggcgcgc tggggctgga agcgttatcg cgctacgctg
    36841 cgggggcaac gttgattgag atggatcgcg tggtttctca gcagttaatt aagaatctgg
    36901 cgacactaaa agcaggcaat gcacgcgtgg tgaacagcaa cgcaatgtca ttcctggcgc
    36961 aaaaaggcac accgcataat atcgtgtttg tcgatccacc gttccgccgt ggcttgttag
    37021 aagagacgat aaatttactg gaagataacg gctggctggc tgacgaagcc ctgatttatg
    37081 tcgaaagcga agtcgaaaac ggtctgccca ctgttccggc aaactggtca ttacatcggg
    37141 aaaaagtggc gggtcaggtg gcttatcggc tgtatcaaca cgaagcacaa ggagaaagtg
    37201 atgctgatta atattggtcg tttgttaatg ctctgcgttt ggggattttt aatcctcaat
    37261 ctggtgcatc ccttcccgcg cccgctaaat attttcgtta acgtggcgct gatttttacc
    37321 gtgctgatgc atggtatgca gctggcgcta ttgaaatcca ctttaccgaa agatggcccg
    37381 cagatgacca ccgccgaaaa ggtgcggatt ttccttttcg gcgtgtttga actgctggtc
    37441 tggcagaaga aatttaaagt taaaaaataa ccttattgtt cgccaacaaa gctaacaaag
    37501 cgcgttcctt tatagctcag cgtaccttta tcgcccacgg tcagggcgtg gtactgctgg
    37561 gcgtcgaggc gaaacgtctg ctccattcct ccgctttgcg gtttgaagct tgcctcatag
    37621 cgcatactgg tgcctgccgg agtcacttcc tgctggcgcg aacggcgatc gttaatcggt
    37681 ttttcccgct tgttgctcac caccaccagc ttttgctgga gcggagccat atcattatca
    37741 gctttttccc gtcgctgttg cataaaacga aacgatgcgg cgacgacaat taagccaatg
    37801 ataacaataa agaaaagagg tggtttactc atctttatcc ctcatcggaa aatgcggaat
    37861 taagcatacc ctgccagtta tggtgttgtc atccgtccac cctcgccact aaactggaag
    37921 caagaccgta ggcattccgc ttacgaaaaa ataacgaatt caaggaacta agatgctttg
    37981 gtcgtttatc gctgtctgtc tttccgcatg gctatctgtg gatgcatcgt atcgtgggcc
    38041 aacctggcaa cgctgggtgt ttaaaccgtt aacccttctt ctcctgctgt tactggcctg
    38101 gcaagcaccg atgttcgacg ccattagcta tctggtgctg gcgggactgt gcgcctcact
    38161 gctgggcgat gcgctaaccc tgttgccacg tcaacgtctg atgtacgcca tcggcgcgtt
    38221 tttcctctcg cacctgctgt acaccatcta tttcgccagt cagatgacgc tctctttctt
    38281 ctggcctctg ccactggtgt tgctggttct gggtgcgctg ctactggcga ttatctggac
    38341 gcggctggaa gagtaccgtt ggcctatctg cacgtttatc ggcatgacgc tggtgatggt
    38401 gtggctggca ggtgaactgt ggttcttccg tccgaccgct ccggcgctct ctgcgtttgt
    38461 tggcgcttcg ttgctgttta tcagtaactt tgtctggctg gggagccact atcgccgacg
    38521 cttccgtgcg gataacgcga ttgctgcggc ctgctacttt gccggtcact tcctcatcgt
    38581 ccgctcactg tatctctgat aaaacttgac tctggagtcg actccagagt gtatccttcg
    38641 gttaatgaga aaaaacttaa ccggaggatg ccatgtcgac acctgacaat cacggcaaga
    38701 aagcccctca atttgctgcg ttcaaaccgc taaccacggt acagaacgcc aacgactgtt
    38761 gctgcgacgg cgcatgctcc agctcgccaa cgctctctga aaacgtctcc ggcacccgct
    38821 atagctggaa agtcagcggc atggactgcg ccgcctgtgc gcgcaaggta gaaaatgccg
    38881 tgcgccagct tgcaggcgtg aatcaggtgc aggtgttgtt cgccaccgaa aaactggtgg
    38941 ttgatgccga caatgacatc cgtgcccagg ttgaatctgc ggtgcaaaaa gcgggctatt
    39001 ccctgcgcga tgagcaggcc gccgacgagc cacaagcatc gcgcctgaaa gagaatctgc
    39061 cgctgattac gcttatcgtg atgatggcaa tcagctgggg tctggagcag ttcaatcatc
    39121 cgttcgggca actggcgttt atcgcgacca cgctagttgg gctgtacccg attgctcatc
    39181 aggcattacg gctgatcaaa tccggcagct acttcgccat tgaaacctta atgagcgtag
    39241 ccgctattgg tgcactgttt attggcgcaa cggctgaagc tgcgatggtg ttgctgctgt
    39301 ttttgattgg tgaacgactg gaaggctggg ccgccagccg cgcgcgtcag ggcgttagcg
    39361 cgttaatggc gctgaaacca gaaaccgcca cgcgcctgcg taacggtgag cgggaagagg
    39421 tggcgattaa cagcctgcgg cctggcgatg tgattgaagt cgccgcaggt gggcgtttgc
    39481 ctgccgacgg taaactgctc tcaccgtttg ccagttttga tgaaagcgcc ctaaccggag
    39541 aatccattcc ggtggagcgc gcgacgggcg ataaagttcc tgcaggagcc accagcgtag
    39601 accgtctggt aacgctggaa gtgctgtcag aaccgggtgc cagcgccatt gaccggattc
    39661 tgaaactgat cgaagaagcc gaagagcgcc gcgcccccat tgagcggttt atcgaccgtt
    39721 tcagccgtat ttatacgcca gcgattatgg ccgtcgccct gctggtaacg ctagtgccgc
    39781 cgctgctgtt tgccgccagc tggcaggagt ggatttataa aggtctgacg ctgctgctga
    39841 ttggttgccc gtgtgcgtta gttatctcaa cgcctgcggc gataacctcc gggctggcag
    39901 cagcagcgcg tcgtggggcg ttaattaaag gcggtgcggc gctggaacaa ctaggtcgtg
    39961 ttacccaagt ggcgtttgat aaaaccggta cgctgaccgt cggtaaaccg cgcgttaccg
    40021 cgattcatcc ggcaacgggt attagtgaat ctgaactgct gacactggcg gcggcggtcg
    40081 agcaaggcgc gacgcatcca ctggcgcaag ccatcgtacg cgaagcacag gttgctgaac
    40141 tcgccattcc caccgccgaa tcacagcggg cactggtcgg gtctggcatt gaagcgcagg
    40201 ttaatggtga gcgcgtgttg atatgcgctg ccgggaaaca tcccgctgat gcatttgctg
    40261 gtttgattaa tgaactggaa agcgccgggc aaacggttgt gctggtagta cgtaatgatg
    40321 acgtgctggg tatcattgca ttgcaggata ccctgcgcgc cgacgctaca actgccatca
    40381 gtgaactgaa cgcgctgggg gttaaaggag tgatcctcac cggcgataat ccacgcgcag
    40441 cggcggcaat tgccggggag ctggggctgg agtttaaagc gggcctattg ccggaagata
    40501 aagtcaaagc ggtgaccaag ctgaatcaac atgcgcccct ggcgatggtc ggtgatggta
    40561 ttaacgacgc gccagcgatg aaagctgccg ccatcgggat tgctatgggt agcggcacag
    40621 acgtggcgct ggaaaccgcc gacgcagcat taacccataa ccacctgcgc ggcctggtgc
    40681 aaatgattga actggcacgc gccactcacg ccaatatccg ccagaacatc accattgcgc
    40741 tgggactgaa agggatcttc ctcgtcacca cgcttttagg gatgaccggg ttgtggctgg
    40801 cagtgctggc agatacgggt gcgacggtgc tggtgacggc gaatgcgtta aggttgttgc
    40861 gcaggagata aggcaaaccg atcgcaacat tgagcgcgat cggtcccctc gcccctctgg
    40921 ggagagggtt agggggtgag gaaaaggcgg catcgaagcc aatcagcccc tatcaaccgc
    40981 ctttacgaat caaataacga taaggcagtc catccgtctc tttcgcaacc agttcgtgtt
    41041 ccataaaggt acaaaaccca ggaatatcgc gggtagtggc cggatcgtcg gcgataatca
    41101 gcaacgtttc gccaggctgc atattgcgca cggttttgcg caccatcatc accggttccg
    41161 ggcagcgcag gccaagcgcg tcgagtgtgt ggtcagggct ggaaaagaga tcggtcattt
    41221 tcttctcatc acttaaaaaa acggcgctag tttacgccct gtgagtccgt aagcaaacca
    41281 ggttaacgat tgcgtgaaaa ttaaccattg cattgtcaac gtaaagcagt atcatgcggc
    41341 ggctcgaaaa aagggtaagc acgttattat gttaaggtaa cagacgtgtc gtacgtattg
    41401 ggttccctca ccccaatggt taatcaaaaa ggtacaatat gaacgttttc tcgcaaactc
    41461 aacgctataa ggcgttgttc tggttatcgt tatttcatct tctggtgatc acctccagta
    41521 actatctggt tcagcttccc gtctccattt ttggtttcca taccacctgg ggcgcgttta
    41581 gctttccgtt tatttttctt gctaccgacc tgaccgtgcg tatttttggc gcaccgctgg
    41641 cccgacgcat tatcttcgcg gtaatgatcc ctgcgttatt aatctcctac gtcatctcgt
    41701 cgctattcta tatgggttcc tggcagggat tcggcgcact cgcccacttc aacctgtttg
    41761 tcgcccgtat cgccaccgcc agtttcatgg cctacgcgct ggggcaaatc ctcgacgtgc
    41821 acgtttttaa ccgcctgcgt cagagtcgcc gctggtggct ggcaccgaca gcgtccacac
    41881 tgttcggtaa cgtcagcgac acgctggcct ttttcttcat tgccttctgg cgtagcccgg
    41941 atgcctttat ggctgaacac tggatggaaa tcgcgctggt cgattactgt ttcaaagtgt
    42001 taatcagtat cgttttcttc ctgccaatgt atggcgtatt actcaatatg ctgttgaaaa
    42061 gactggcaga taaatccgaa atcaacgctt tgcaggcgag ttaaaggttc gtaatcagag
    42121 ttgtgataag atggatgaat gagccgttat ggccgtttat cgaaaggaag aagtcaatgc
    42181 gcaatctggt taaatatgtc ggaattggcc tgctggttat ggggcttgcg gcctgtgatg
    42241 ataaagacac taacgctacg gcgcagggtt cggtcgcgga aagtaacgct accgggaatc
    42301 ccgtcaacct gcttgatggc aagttaagtt tctcgctgcc agcggatatg accgaccaga
    42361 gcggtaagct gggaacgcag gccaataaca tgcatgtctg gtccgacgcc accgggcaga
    42421 aagcagtcat cgtcatcatg ggcgatgatc cgaaagaaga tctggcggtg ttggcgaagc
    42481 gtctggaaga tcagcaacgt agccgcgatc cgcagctgca agtggtaacc aataaagcca
    42541 ttgagctgaa aggtcacaaa atgcagcagt tagacagtat tatctccgcg aaaggccaga
    42601 cggcgtactc ttccgttatt ctgggtaacg tgggtaatca actgctgacc atgcaaatta
    42661 cgctgccagc tgacgatcag caaaaagcgc aaaccaccgc agaaaacatc attaatacgc
    42721 tggttattca gtaagtttta agatgatgag gcggcctcag ggacgtgttc cggaggccgt
    42781 ttttttaatc gccacgtcag taataacgcg attgcgacca gtcccgccgc cgccagataa
    42841 atcaccggta cgcccgccca gctcatcacc agcccagcca gtggtccagt cacgccaagc
    42901 gataaatcca taaatacggt gtaagttgcc agcgccgccc cctaattttg ctgcggaacc
    42961 gcttttaccg ccactacacc caatgccggg aacaccagcg aaaacccggc ccccgccagt
    43021 aagacgccga ttttcgccat ccacggcata gtcgccacgc caaccagtag caggccgatt
    43081 atctcaacgc taaagcaaat catcgctacg tttaagccac cgatacggtt aatgccgtta
    43141 gggaataaca aacgcgtacc gacaaacgca cagctaaaca gcgtcagcgc gaaagccgca
    43201 ccgtcccaac ctttagcgtc ataaaacagc gtgataaagg tggcgatgac gccaaatccg
    43261 gcggaagcca gtgccagcgc cataccgtac agccagacgc gcccaagcac cgcgcgaaac
    43321 ggcagcggtt tgcctttact ggcttttacc gtcggacgcg ggatcgccaa caaaatggcc
    43381 accagcgcca cgcccataat gattaacgct aacgcctgca agccgcccca gtgataaaac
    43441 acgacgccta acggcgcacc catcgccatc gccccgtaag tgacaatgcc gttccacgaa
    43501 atcacccgcc cgatatgcag cgagccaacc actccaacgc cccatagggt cgatcccgtt
    43561 ccggcaaaac tttgcccaat cccaaggatg acgcgcccca ggcaaagtaa taacaggctg
    43621 atgacaggca gactggcggt taatcctgcc gtcagatacc ccagaccgct caaaaagcag
    43681 ccgcataaac cgaagacgac aatctttttg ggtcccagca aatcggcgta acgtccggca
    43741 tgagggcggc tcagcaaggt ggcgaaatat tgcaggctga taaccaatcc tgcccagaag
    43801 gcgctaaagc ccatcacatc atggacatag cccggtaata cagcgagcgg caacccgatg
    43861 gtgaggtagc tggcgaagtt aaacatgaca atggagacaa tgcgcaaatt caggcgcaat
    43921 ccgtttagcg cgggttcggc tacgggttcg ggcatgagga tcaccacatt tttacaacag
    43981 tgtttcattt ttaccatgtg ctgacgtgaa aatcagcagt aagaatcaga atattgctgg
    44041 cgtgactccc gctacactta acacaaaaag tcacaaggaa gccccaatgg aaacccctca
    44101 acccgataaa acgggcatgc acattctgct caagttggcc tcgctggtag tgatcctcgc
    44161 gggcattcac gcagcggcag atatcattgt gcagctgtta ctggcgctgt tttttgccat
    44221 cgtcctcaac ccgctcgtta cctggtttat tcgtcgggga gtacaacgcc ccgttgccat
    44281 tacgattgta gtggtggtga tgctgatcgc actaaccgcg ctggtcggcg tactggcggc
    44341 atcgtttaac gaatttatct ctatgctgcc gaagtttaat aaggagctga cgcgcaaact
    44401 ttttaaattg caggagatgt tgccttttct taatttgcat atgtcgccgg agcgaatgct
    44461 gcagcggatg gactcggaaa aagtggttac cttcaccaca gcgctaatga ccgggctttc
    44521 cggggcaatg gcgagcgtgc ttttgctggt gatgaccgta gtttttatgc tgtttgaagt
    44581 gcgccacgtc ccttacaaaa tgcgttttgc gctgaataac ccacagattc acatcgcagg
    44641 attacaccgc gcacttaaag gcgtttcgca ttatctggcg ttaaagacgc tactcagttt
    44701 atggacaggt gtcatcgtct ggctggggct ggcgctaatg ggcgtacagt ttgcgctgat
    44761 gtgggcagta ctggcgtttt tgctcaacta cgtgcccaat atcggcgcgg taatttccgc
    44821 cgtaccgcca atgattcagg tgctgctgtt taatggtgtt tacgaatgta ttctggtcgg
    44881 cgcattgttt ttagtggtcc atatggtcat cggcaatatt ttagaaccac ggatgatggg
    44941 ccatcgcctg gggatgtcca ccatggtggt atttctttca ttgttaatct ggggatggct
    45001 gctcggcccg gtagggatgc tactttcggt gccattaacc agcgtgtgta aaatctggat
    45061 ggaaacgacg aaaggtggca gcaaactggc gattttactg gggccggaca gaccgaaaag
    45121 tcggttaccg ggatgaggcg acaagtgata cgatacgcac tttcattttc cattaaacgt
    45181 tggccctgat atgtatcgga tagttctggg gaaagtttcg accttaagcg cagctccact
    45241 gccaccgggt ttacgcgagc aagcaccgca aggtccacga cgcgaacgct ggctggcggg
    45301 gcgtgcattg ctttcgcaca cgctttcccc gctaccggag atcatctatg gcgaacaagg
    45361 caaacctgca ttcgcgccgg aaacgccgct atggttcaac ttaagccata gcggtgacga
    45421 tatcgctctg ctgttgagcg atgaaggcga agtcggctgc gatatcgaag tgattcgccc
    45481 gcgcgccaac tggcgctggc tggcgaacgc cgtgttcagc ctcggggaac acgctgaaat
    45541 ggacgccgtg catcctgatc agcaactgga aatgttctgg cgcatctgga cgcgcaaaga
    45601 agccatcgtt aaacagcgtg gcggcagcgc ctggcaaatc gtcagcgtag atagcaccta
    45661 tcactcctcg ctgtcagtca gccattgtca gctagaaaat ttaagccttg cgatctgcac
    45721 ccccaccccc tttacgctca ccgccgacag tgtgcaatgg atcgattcag ttaactgatc
    45781 cgcccacccg actgcccatc tattgatcca gaacaggtaa tcagtatgac gaatacttaa
    45841 aatcgtcata cttatttccg ccatctattt taatccattg gggttaccat gctctccaca
    45901 ctccgccgca ctctatttgc gctgctggct tgtgcgtctt ttatcgtcca tgccgctgca
    45961 ccagatgaaa tcaccaccgc ctggccggtg aatgtcgggc cactaaaccc gcacctttac
    46021 acgcctaacc agatgttcgc ccagagcatg gtttatgaac cattggtgaa atatcaggca
    46081 gacggttcgg tgatcccgtg gctggcaaaa agctggactc attcagaaga tggtaaaacc
    46141 tggaccttca ccctgcgtga tgacgtgaaa ttctccaacg gtgaaccgtt cgatgccgag
    46201 gcggcggcag aaaacttccg cgcagtgctc gataaccgtc aacgtcacgc ctggctggag
    46261 ctggcaaacc agattgttga tgttaaagca ctcaataaaa cagagctgca aattaccctg
    46321 aaaagcgcct actatccttt cctgcaagaa ctggccctgc cccgtccttt ccgttttatc
    46381 gctccctcgc agtttaaaaa ccatgaaacc atgaacggaa ttaaagcgcc gattggcacc
    46441 ggaccgtgga ttttgcagga atcgaaactg aatcagtacg atgtcttcgt ccgtaacgaa
    46501 aactactggg gcgaaaagcc agcgattaaa aagatcacct ttaacgtcat cccggacccg
    46561 actacccgcg cggtggcgtt tgaaactggc gatatcgacc tgctgtacgg aaacgaaggg
    46621 ttattaccgc tcgatacctt cgcccgcttt agccagaatc cggcttacca cacccaactg
    46681 tcacagccgc tcgaaaccgt gatgctggcg ctcaataccg ccaaagcccc caccaacgag
    46741 ctggcagtac gtgaagctct taattacgcg gtaaacaaaa aatcgctgat tgataacgcg
    46801 ttatatggca cccagcaggt cgccgacacc ctgtttgccc cttctgtgcc ttacgctaat
    46861 ctcggcctga aaccgcgcca gtacgatccg cagaaagcga aagagttgct ggaaaaagcc
    46921 ggttggacgc tgcctgcggg caaagacatc cgcgagaaaa atggtcagcc gctgcgcatt
    46981 gaactgtcgt tcatcggcac cgatgcgtta agcaaatcga tggcggaaat cattcaggct
    47041 gatatgcgcc agattggcgc agatgtctcg ctgattggcg aagaagagag cagtatctat
    47101 gctcgtcagc gcgacggtcg ttttggcatg attttccacc gcacctgggg cgcgccatac
    47161 gatccacacg ccttcctgag ttcaatgcgc gtaccgtcgc acgctgactt ccaggcacag
    47221 caaggattag ccgacaagcc gctgattgat aaagagatcg gcgaagtgct ggcgacccat
    47281 gacgaaacgc aacgtcaggc gctgtatcgc gacattctga cccgtctgca tgacgaggcg
    47341 gtttatctgc ctatcagcta catctcgatg atggtggtat caaaaccgga gctgggtaat
    47401 atcccctacg cgccgatcgc caccgaaatt ccgttcgaac agattaaacc ggtgaaacct
    47461 taatgttgcg ttacgtatta cgccgctttc tgctgctgat cccgatggtg ctcgccgcct
    47521 cggtgatcat ttttctgatg ctgcgcctcg gtaccggcga cccggcgctc gattatctgc
    47581 gtttatctaa cctgccgccg acgccggaga tgctggcctc tacccgcacc atgctcgggc
    47641 tggatcagcc gctgtacgtc cagtacggta cctggttgtg gaaggcgctg catcttgact
    47701 ttggtatctc attcgccagc caacgcccgg tactggacga tatgctgaac ttcctgcccg
    47761 ccacgctgga acttgcaggt gcggcgctgg tattaattct gctcacttcc gtaccgctcg
    47821 gtatctgggc ggcgcgccac cgcgatcgtt tgccggattt cgccgtgcgc tttatcgcgt
    47881 ttcttggcgt gtcgatgccc aacttctggc tggcattttt gctagtgatg gcgttttcgg
    47941 tgtatctgca atggctaccc gcgatgggct acggcagctg gcagcacatc attttacctg
    48001 cggtttccat cgcctttatg tcgctggcga ttaacgcgcg actgctgcgc gccagtatgc
    48061 tggacgtcgc cggtcagcgt cacgtcacct gggcgcggct acgcggcctg aatgacaaac
    48121 agaccgaacg ccgccatatt ctgcgcaatg cctcgctgcc gatgatcacc gccgtgggga
    48181 tgcacattgg cgaattgatt ggcgggacga tgattatcga aaacatcttt gcctggcctg
    48241 gcgtcgggcg ctatgcggtg tcggcgattt ttaaccgtga ctatccggtg attcagtgct
    48301 ttacgctgat gatggtggtg gtttttgtgg tctgtaattt gattgtcgat ttgctcaacg
    48361 ccgcgctgga cccgcgcatt cgtcgtcatg aaggagcgca cgcgtgaact ttttcctctc
    48421 ttcccgctgg tcggtacgcc tggcgctgat cattatcgcc ctgctggcgc tgattgcgct
    48481 caccagccag tggtggctgc cgtacgatcc acaggcaatt gatttgccgt cgtgcctgct
    48541 ttcgccggat gcgcagcact ggctgggcac cgatcactta ggtcgcgata ttttctcgcg
    48601 gctgatggca gcgacccgcg tgtcgctcgg ttcggtaatg gcctgcctgc tgctggtgct
    48661 gatattaggg ctggttattg gcggcagcgc cgggttgatt ggcgggcgcg ttgatcaggc
    48721 caccatgcgc gtcgccgata tgtttatgac cttcccgacc tcgattctgt cgttctttat
    48781 ggttggcgtg ctcggcaccg ggctgaccaa cgtaattatc gccatcgccc tgtcgcactg
    48841 ggcgtggtat gcacgcatgg tgcgcagctt ggtgatttca ctacgccaac gcgagtttgt
    48901 gctggcgtca cggctttccg gtgcgggcca tgtgcgggtg tttgtcgatc atctggcagg
    48961 cgcggtgatc ccttcgctgc tggtgctggc aacgctggat atcggccata tgatgctgca
    49021 cgtcgcgggg atgtctttcc ttggcctcgg tgtgaccgcg ccgaccgccg aatggggcgt
    49081 gatgattaac gacgcgcgcc agtatatctg gacccagccg ctgcaaatgt tctggccggg
    49141 gctggcgctg tttatcagcg tgatggcctt taacctggtg ggtgacgcac tgcgcgatca
    49201 tctggatcct catctggtga cggagcacgc acactaatgc cgcaacagat tgaactacgt
    49261 aatatcgcgc tacaggccgc gcagccgctg gtacacggtg tatcgttaac cctgcaacgc
    49321 gggcgcgtgc tggcgttagt cggcggtagc ggcagcggga aatcattaac ctgcgccgcg
    49381 acgctgggca ttttgcccgc tggcgttcgc cagacggcgg gggaaatttt agccgatggc
    49441 aaaccggttt cgccttacgc cctgcgcggc atcaaaattg ccaccatcat gcagaacccg
    49501 cgcagcgcct ttaatccact gcacaccatg cacacccacg cgcgggaaac ctgcctggcg
    49561 ttagggaaac ccgccgatga cgctacgctt accgctgcca tagaagcggt ggggctggaa
    49621 aacgccgcgc gcgtgctgaa gctgtacccg ttcgagatga gcggcggcat gttgcagcgc
    49681 atgatgattg cgatggcggt gctgtgtgaa tcaccgttta tcatcgccga tgaaccgacc
    49741 accgacctcg acgtggtagc acaggcgcgc atcctcgatc tgctggaaag cattatgcaa
    49801 aaacaagcgc cgggaatgct gctggtcacc catgatatgg gcgttgtggc gcgtctggcg
    49861 gatgacgtgg cggtgatgtc acaaggtaaa attgtcgaac agggcgatgt agaaacgctg
    49921 tttaacgccc ccaaacatac ggtgacgcgc agcctggttt ccgctcatct cgccctctac
    49981 ggtatggagc tggcatcatg accttactta acgtctgcgg cctttcccat cactatgccc
    50041 acggtggatt tagcggaaaa catcaacatc aggcggtgct gaataacgtt tccctgaccc
    50101 tgaaaagcgg cgaaaccgtc gccctgctgg gacgcagcgg ctgcggaaaa agcaccctcg
    50161 cgcggctgct ggtaggctta gagtctccct cacaagggaa tattagctgg cgcggcgaat
    50221 cgctggcgaa acttaatcgc gcccaacgta aagccttccg ccgtgatatt cagatggtgt
    50281 ttcaggactc catcagtgcc gtgaatccgc gcaaaaccgt gcgcgagatc ctgcgtgaac
    50341 cgatgcgcca cctgctatca ctgaaaaaat ccgaacaact ggcgcgcgcc agcgaaatgc
    50401 tgaaggcggt cgatctcgat gacagcgttc tcgacaaacg cccaccgcag ttaagcggcg
    50461 gccagctcca gctcgtctgc ctggcacgcg cgctggcggt cgaaccgaaa ttgctgattc
    50521 tggatgaagc cgtttctaac cttgatctcg tgttacaggc gggcgtcatt cgcctgctga
    50581 aaaatctaca acaacagttt ggcactgcct gcctgttcat cactcatgac ttacgcctgg
    50641 tggaacgctt ttgccagcga gtgatggtga tggacaacgg acaaatcgcc gaaacccagg
    50701 cggtgggaga taaattaacc ttttcctctg acgccggacg tgtgctacaa aacgcggtat
    50761 tacccgcatt ccccgtgcgc cgtcgcacct cagaaaaggt ttaacgccaa tgcaacgagt
    50821 caccatcacg cttgatgacg atttactgga gacgctggac agcctgagcc agcgtcgtgg
    50881 ttataacaac cgttccgaag ctatccgcga tattctgcgt agtgccctgg cgcaggaagc
    50941 tacccagcag cacggcacac aaggtttcgc ggtgctgtcg tatgtgtatg aacacgaaaa
    51001 acgcgactta gccagccgta ttgtttccac ccagcatcat caccacgacc tctccgtcgc
    51061 cacgctacat gtgcacatca accacgacga ctgtctggaa atcgccgtgt tgaaaggtga
    51121 catgggtgac gtgcagcatt ttgccgatga cgttatcgcc cagcgcggcg tgcggcacgg
    51181 gcatttgcag tgcctgccga aggaagattg agttatttct aaaacaagga aagaaaagat
    51241 attaactgaa cttaatttat aaaattaagt tcagttgcat agatagatcc tgtaaaggca
    51301 gatattctgc gtaatgtacg tcgctaaaat ttataaatga ttcaaacgct cattcagtgt
    51361 ttcaataata taggtattaa cagagacttg ttgctgcgca gccgcattat taaggcgttc
    51421 actcagcgat tctggatagc gtaacgtaaa ggtcttaatc ttttctgtac gcgcatacgg
    51481 ctcaataccc gcagctttac aatcctcaag atactctcgc agggaaagct ctccttcttt
    51541 ttgcagcccc tgaatactgt cagagacaaa atcacaataa cctgacaagc ccaggaattt
    51601 gccacgaaat gcattcagtt ctggcacgta ggtaataaca gctggttgcc cggcaatctc
    51661 catagagttt ggcgtcttca gtttgatcat ggttttaccc ctatacttag taaccagtca
    51721 cgcacgcttt ctacagcgcc tttatcggta tctggcgacg gatgaggccg atggaaacac
    51781 gcaacgctca tatttagtat gaacttgcaa cgcgaacctc ttccttcctt aatttctccg
    51841 cctaatgctt taaccagtga ctctatatca gaccacttga tcccttgagg aacaggtgtt
    51901 ttaaatattt gatccaatgt atttttttgt ttcttccgta aagcctgtac cttggttctc
    51961 atggcatgtc cttatgactt cactttatga catcacagta tgaagtcaag tttcccttcc
    52021 ttacgccatt gtcccaatcg tcttcctgaa tcgcagcagt gcgatggtga aaaacgcgcc
    52081 gccaattgcc atcagcgtca ggaactgcgg ccagacgatt tcgaatccgg caccccggta
    52141 gaggatggcc tgcgcgaggc taacaaagtg tgtcgtcggc atggtcagca taatgtcctg
    52201 caccatctgc ggcatacttt cgcgcggcgt ggatccaccg gaaagcattt gcagcggcag
    52261 cagcaccaga atcaccagca gccccagttg cggcattgaa cgcgctatcg tccccataaa
    52321 aatgccgatt gacgtggtgg caaacagact gagcgccacg cccagcataa acagcgggat
    52381 cgagccttca atcggtacgc ccagtacacc tttcaccatc agcaccagcg ataatcccga
    52441 taccaccagc accaccagcc ccatcgacca gatcttcgcc atcatgatct caaacggcgt
    52501 tatcggcatc accagtaaat gttccaccgt gccgtgttca cgctcgcgga tcagcgccga
    52561 tccggtcaat acaatcgcca gcatggtaat gttgttgatg atcgccatca ccccgccaaa
    52621 ccacgcggga tcaaggttcg ggttaaagcg catccgggtt tccagcgata ccaacggttc
    52681 gctgttatca cggtagcgcg cgacaaagct gttcacttca ccgttgataa tattctggat
    52741 atacccattg ccggtaaatg cctggctcat gcgcgtggca tcgacgttca cctgaatatc
    52801 cggctggcgt ccggcgagga catcacgctg aaaattaggc ggaatattta tcgcgaaggt
    52861 atagcgtccg gcgtccagtc cggcatccat ctcatcggcg gtgatcatct ccggtggcaa
    52921 aaaccacgga cgatagaagc tgttaacgat ccggttcgat aactgcgatt gatccatatc
    52981 ggcaatggcg atcggcgcga ggttcaacga tcctggcgta acggtcgctg acgaatacac
    53041 cgacaccgta aacgagaaga caatcagcgt cagcatcgct ttatcaccga gcagactgcg
    53101 caactctttg atacccagat taaaaatatt gcgtaactgg cgcatcatcc ctcctgtttt
    53161 ttcagcagca ggatgcttaa gcccatcacc agcgggatgg ctatcagcaa cgggataaaa
    53221 agttgccaca aatcagtcag atccagcgct ttcgagaacg ttccgcgggc gatagtcaga
    53281 aaatgactgg tcgggtaaac ctcgccgatc caacgtccag gcccttccag cgaagctacc
    53341 ggatcgatca tcccggaaaa ctgtgtcgcc gggatcaatg tgataatcgc cgttccgaaa
    53401 atggcggcaa tctggctttt cataaaggtg gagatcagca gccccattcc agtggcaatg
    53461 atgatataca gcagcgccgc caaggtgagc gtcaggaaac tgcctttatg cggtacgcca
    53521 aacacaaaca ccgacaggcc gcagagcagg aaaaagttca gcatccccag cgcgatgtac
    53581 ggcagctgtt tgccaagcaa aaattcgcta cgggtggtgg gggtcacgta aaggttgata
    53641 atcgacccta gctctttttc ccgcaccacg ctaagggcgc ttagcattga cgggatcatc
    53701 atcagcagaa gcgggatcac cgccggaaca atcgctggca ggctttttac gtccgggtta
    53761 tagcgataac gcgtctcaat gttcatcagc ccgctttggc tggcgggtgt cgattgtcgg
    53821 ctcgccacat cctgaagcca actctggtgc attgcctgca cgtaaccttt taccgtttcg
    53881 gcacgactgg gcatcgctcc gtcgatccag acgccgagtt ccacaggcgt accacgcgcg
    53941 atatcgcgcc cgaaattggg cgggatctca atggccaccg tgatatcgcc cgcacgcatc
    54001 cgacgatcaa gctcgtcata actggtgagc ggcggctgtt cgataaagta acgggaaccg
    54061 gagaggttga gtgtccacgc ctggctactg acggtctggt cgcggtcgag caccgcaaag
    54121 cgcaggtttt ccacatccat actgatgccg taacccatta tcagcatcag gatcaccgtt
    54181 cccatcagcg ccagcgtcga acgtactgga tcgcgtcgca gttccagcgc ttcgcggcgg
    54241 ctgtagctaa acagacggcg caggctaaat ccctgacgcg gcgcgtgggt ggtgtcgtgt
    54301 accacgggcg gcgcttcggc ttcgttgctc tgccctgccg cttcctgcaa ataggcgata
    54361 aatgcctctt ccagactggc ggctccgcgt ttctcaacca gttcctgcgg tgtaccgctg
    54421 gcaagcactt ttccggcgtg catcagtgag atgcggtcgc aacgttccgc ttcgttcata
    54481 aagtgggtgg agatgaagat agtcactttg tcctggcgcg agagatcgac catcaactgc
    54541 cagaacatat ccctcgccac cggatcgaca ccagaagtag gctcatcgag gattaacatc
    54601 tccgggcgat gaatcaccgc caccgccagc gaaagccgct ggcgaatgcc gagcggcaat
    54661 gactccggca gaacatcttc aacgtcgttg agcttaaaac gctcgctcat ttcagccact
    54721 cgcgcgggaa tttccgcttc cgggatgtga aacaaacggg catgtaactc caggttttgc
    54781 cgcacggtga gttcgttata gagcgaaaac gcctgcgaca tatagcccac acgacggcgg
    54841 gtatcgatat cttttggatc aaccggttgc ccaaatagcc acgcctcacc ttcgctggcg
    54901 ggcagcagtc cggtaagcat tttcatggtg gtggatttac cgcagccgtt cgaaccaagg
    54961 aatccaaaaa tctccccgcg tggaatgcgg aaattgacgt gatcaacggc aacgaaggaa
    55021 ccaaaacgca tggtcagatc gcgcgcttcg atggcaatct ctgcgttttc aggttgatac
    55081 ggtgggatca ctaccgcctg atgcgcctgg cgttgcgctt gcggtaacag attaataaat
    55141 gcttcttcca gcgtggcgct ttgcgtttgc tgccgcagct cttcggcgct gccggttgcc
    55201 agcacttctc cggcattcat cgctaccagc cagtcgaagc gttcggcctc ttccatatag
    55261 gcggtggcga ccagcacgct catattgctc tgccgctggc gaatactgtc gatcagatcc
    55321 cagaactggg cgcgggagag cgggtcaacc cccgttgttg gctcatcaag gatcaacagt
    55381 tccgggtcgt ggattaacgc gcagcacagc ccaagttttt gcttcatccc gccggagagt
    55441 ttacctgccg gacgatcgcg aaacggtgct aacccggtgc tggtcagcag ctcattaatt
    55501 cgcacttccc gctccgcttt atcgtgaccg aacaggcgag cgaaaaaatc gacattttca
    55561 tacaccgaca aggtgtggta gaggtttttg cccagcccct gcggcatcca ggcgatgcgc
    55621 gggcagacgt ctcggcgatg cttcgggtcg tgcatatcgc cacccagcac caccacattg
    55681 ccctgctcaa tgacgcgggc accggaaatc agtgacaata aactcgactt cccgacgcca
    55741 tccggaccaa tcagcccgac catgcaacgg gccggaatat cgagagtgat attgttcagc
    55801 gcaacggttt ttccataatg ctggctcacg cccgccagtt gcgcgacagg cgggacagga
    55861 accagttcca gatgcgtcat tgcggcaacc tcaccacgag gtcgtcaggc cacggaagtt
    55921 cttcattcac ccgcacccac gctacgcccg gtaaaccggt tttgacatat tccagatgct
    55981 gctggagtaa ttccggtggg atacgcgctt tgacgcggaa catcagtttc agccgttcat
    56041 cgctggtttc gacggttttt ggcgtgaact gggcgacact ggcgacaaaa ctgatggttg
    56101 caggaatacg cagatctggc gcggcatcga ggatcagccg ggcttcaccg cccagtttca
    56161 gcgtgcccgc ctgttcggtt ggcaggaaga aggtcatata gacgtcgctg agatcgacca
    56221 tattcagcac ccgaccgcct gccgccagca cttcccctgg ctcggcaacc cgatactgca
    56281 cgcgtccgtc acgcggggct ttcagttcac tgtcatcgat atctgcggca atgcgccgtt
    56341 cagtggcttg cgctgcttcg acccgagttt gcgcctgaat gatattggtg cgtgctgctt
    56401 ctatagccgc tttagaagcc gaaacctggg ctttcgctga ttccagcgca gctcgggcgc
    56461 tctcagcggc ggcgcggtcg tcatccagct gttgcgcaga aatagcccct cgttgggcga
    56521 gcgagcggga acgcgtatga cgtttggcaa cagagtccag ttcggcctgg cgttgattga
    56581 ccagcgactg tgcggcacga gtttcacttt gtcgttgctc cagcaaagcc tgcgcggcgg
    56641 caacggcgct ttgtgcctct ttgatttgcg cgatggcttc cagtcgctgt tcctgcaaca
    56701 cgcgagtatc catcttcgcc agcacttcgc cttcgcgaac aaactggcct tctttcacca
    56761 gaatggtgtc gatacgcccg gcagttttgc tggcgatatc cacttccgtc gcttcaatgc
    56821 gcccattgct gacagcaaag ccttccggca cacccgccgg gcgcaacagc caccacgcca
    56881 cgacggcagc caccgccagt gccccgacaa cccaccacgc cagatggcgc ttactcttat
    56941 ccataatcga cccgccataa tccctgtaag caaacgacgc gtgttacaac tgccgccgaa
    57001 cgacgttcag cggattcttc catccgggat aagaaggaaa cgacaaatac gaccagcgcg
    57061 cagagcattg agcgccaacg gggaaggctt agcgaaaacg ggtagtaatg ttgtcatcat
    57121 cagcattcct ggccgtaaat gaaacacgca tactcataag tgtgatctat aaaactggct
    57181 gacaaaacgg tatccatcct gttggatcgc gtttcacgcc tgttcagttg caggaacaga
    57241 gcattccttt ttacgtttta tcaacagtct caaccagata gtaacattaa tattcatgtt
    57301 gtctatggtt cagagctgta acattgtgcg ccttctaatc aaaggcataa gtccatttct
    57361 gtttttttca tgctggtaag acttccaggg agtggttatc tctggccctg caccagggtt
    57421 aatttggcaa ttatgcatgc tgaacattct tccagcatgt ttactgtcac gggcgttaag
    57481 acctaaaact gtccccgttt cacctatgcg ttctctccca ccatttccgt tatgtgcaac
    57541 aacttgattt atacctaaca tttgtgaaca tgctaatagt gcttcaagtt taggtctgta
    57601 atctgtcgaa acagaaaaat aacgattaaa ggatcgacaa gagatagccc ggctagtttt
    57661 aaactttttg ccattaatta tagcatgatg ctttttatta ataatttccg ctaattcgac
    57721 cggatcgaga gcattggtca tttcatcaat agagacagtt atcggtccta taatgtaacg
    57781 ttttcgctga tcatcaaaag ttatcccatg atgatttgcc attattttgt atttggggtc
    57841 atagaaacat acaggataat cttttatccc gtcgtaggta tcctcatcaa gtggtttcat
    57901 gcgcgtgaag ttttgcatat agttaccatt gcaattcgtt tcatgatttc cagctaatac
    57961 atgaacattt ttattgatac tcattatgcg ttttaacaaa tcgatgataa attgaacgcc
    58021 acagatatta ctaaatcgat cgccggtttg gtcgccaaga aacgtacatg gcgtgactgc
    58081 gtctttatta ataaccaaat aaggctgaag actttctaaa acattatctc ttctgttcag
    58141 tatactgatt attgtattaa cattagtgtc aagttgctca ttaaagttct cccttagcaa
    58201 tacgttgaga acttcatctt ctatattaag caactggcta aggaaatttt ctccttcagc
    58261 catgattccc aaatgtcctg ttgcaaaaag cgcgtacaaa actgcaccga ctgagctatc
    58321 ggtatcgcca aaataggtcg gcctggtact tgtgggatca acaggtgaac aattggagaa
    58381 ataggttctg tcagcgaact ggggttttat aattgtttcg aatttaatat caaatacatt
    58441 gtattgcgat atttcccagt tccagatggg tttgtacgac tcagtaactg caacatcatt
    58501 agcattatca tttactgtca tattattaag taaataaaca atgctgcacc aattaatatc
    58561 ggtattatca agagggcaac cgtgattttt actattaatt gtcacaataa actgataaca
    58621 gttattccta cgtagaatat gagcgcgcat gcccgatagc atactatatt tttctgaatg
    58681 attcgtttgt atggtaaatg aaattttgtc atcttgaata gattgttgga aacgttctat
    58741 ttgccaatca tcaatttttt ccacaacttc accgagcatt gtcctggcaa tacttgatat
    58801 atcatcacct ataccatttg caatataccc aaacagttct ttagtgaagt tggtaacatg
    58861 ctcttgcacc atatcatttg ttgcgattgt cgttgtactg ccattagtgc ctgccacagt
    58921 tccaattttc atcttttacc tcatattatt caccgcgagg aatacatagt taacatttta
    58981 tcttatgaca tacacctcat attcgatggg aaaataatta tattttgcac aaatcattca
    59041 gccttaaagc ataatatgaa atttcttatc tcatagcaga gaaagtccct aaagattgta
    59101 gaggcgtcat cagtacatac ctctacaatc gattaagaaa acctatgaaa tactggcaaa
    59161 attaaaaaga gataccacag cactcatgaa gttttattga ggttgaaaac aactaagccc
    59221 tgggacacct tttttatcac cacgttcgtt aggtgctatc ttccaagaat ttagccccat
    59281 ggtataatta tcaatttttt gatgctggtc tacccctaat ccataatggc caaatttctg
    59341 tttagttttt ttgagcgtct gtccacattt gaagatatct tcaggttttg gtctgaaagc
    59401 agtagtgtta ttaaaataat tttcatatac aggtgtgggt gctatagcat tgcttctaat
    59461 tagatggaga agatgatcat ccatgtgttg tttatgtttt ttattaaata tatttgctaa
    59521 ttcctcagga tcaattgggt ctttcatctg attaaatgga acctgcaaag caccgagaca
    59581 ataacattga ctttcttcat ttcttattat tccgtgatga ctggttaaaa ctttagtttc
    59641 tgagttataa ttacaaacat cgagcgtttt aattaagtta tacgtatcac cggcagagag
    59701 tttataattt tctagtcgtg cagtatagtt accattaaaa ttgatttcat gatttccagc
    59761 cagaacgatg acatttttat taatacggct atctttatta ccctccatat ttcgcataga
    59821 atttagcaaa gtaaggatat atttatcacc aaaaatagta ctaaatctat cgcctgtatg
    59881 atccccaagg gggatacaag gggttatcgc atcgtccttt aaaaaaacat gttgatgcaa
    59941 ctcattcaat atcatgtctc tcgtatttaa taaattgtag agcaatttat tatttttatg
    60001 tatgtatgca gatgaagcca tgtcttcgtg ttttaataat tcacataata aactccaacc
    60061 cttttccctg atgcctatat gccctgaaac gagcaatgaa taaagtacag cgcctactct
    60121 tccatctgta tcgccaaaat aagccgtgtt ggcattttgt gggttaatca caccacattg
    60181 tttataaaag tctattttag aaaacacaga tttaacatag ctatcaaact tatccttagg
    60241 aaacgtctct ttagagacat cccaacgcca gtcagcttta aaattaccgc taagaagagt
    60301 aaagaaattt tcaggtatga tagaaagccc attaacttta taaattatgt ctgatttatc
    60361 tccggagaaa ttaataaaat aataaccatc cttacactca atcttaactg taatgggaga
    60421 cgataatttt atcacatggt tattgtctgg tataattgta aagataaacg tgttagtagt
    60481 attatcggca aaactattat attcttgaat atctatagat ggctcactat tgtaaaacca
    60541 tattttctcc atctgaagac tatgttcatt taccctccag tgaccatgtt gcaggaaata
    60601 gatgatatgc atgccaaaat cgttatctat ttgcgaatca ttatccagtt tatcaatttc
    60661 aactatcttt gaacgatgtg ttttatcaac cttgctatgg tgttgatgat tattaacttt
    60721 atctgaatgc ttatctttat ggtttgaagg gatgtactta aatatattgt caattgcttt
    60781 cataaataac ccgcgatatt tttgacctgt tagtaaaaaa taaatgatac atgtctgaaa
    60841 gacatattta cgacgatata aaacaataat gcttatatgt tcgctgattt taagcaccga
    60901 gactatccgg tcttttacta aataatggaa aacaatccat ccctggaaga caattgtaaa
    60961 aaaaacaaca gaataagaat taatcttagg ataaatattt atttatcatg gccttttgac
    61021 cgctaaaaaa tggcactcgg cacagtaaac cccgctatag ttatgctata catttgtctt
    61081 taatgatatc tcgtcgttat attaacaggc tgaaattatc attaatgcac atttcaatat
    61141 tagcagggat acctgaggag tttaaagtga acatatatat cgggtagctt ttcaaattaa
    61201 tccctttgat tataggctta atttgcatcg cgttaggtgg ctttgtgctg gaaagttcag
    61261 ggcaacgtca cgggtcatgt gctgatttct ctggcggcca tatgcctggc attattcact
    61321 accgcattta ttatcatttc gcagctcacg cgcggcgtta atacgtttta caatacattg
    61381 ttccccatta ttggctatgc ggggtcaatt atcaccatga tatggggttg ggcactgtta
    61441 gcaggtaatg atgtgatggc agacgagttt gttgccggcc atgttatttt tggcgttggt
    61501 atgattgccg catgtgtatc gacggtggca gcgtcgtccg gtcactttct gctcattccc
    61561 aaaaatgcag cggggagcaa gagcgacgga acaccggtac aggcttattc ttcattaatc
    61621 ggtaattgcc tcattgccgt tcccgtttta ctcaccctgc tcggtttcat ttggtctatt
    61681 acgctgttac atagtgccga cataactccg cattatgtcg ccttgggtta accgcaatct
    61741 gtgcctgtct aattggccta gtggccacaa tcgtccatca aacacgtaat acgttctcat
    61801 ccaaagagca ctggctgtgg tgttattggg ttatttttct cggctcagtc acggtgctgc
    61861 aggggatata cgtcttagtc agttccgatg caagcgcccg actggctccc ggcattattc
    61921 tcatttgcct cggaatgatc tgttacagca tattctcaaa agtctggcta ctggcactgg
    61981 tctggagacg tacctgttcg ttagccaaca gaataccgat gataccggta tttacctgtc
    62041 tgttttgctt gtttttgtca tcattcctcg cggaaatcgc acaaactgag ctggactact
    62101 ttattccatc tcgggttctg gttggattaa gcgcagtatg ttttacattg ttctctattg
    62161 tttcaatatt agaggccggt tctgcgaaaa aaaattaaaa tttacataat cccagcgtta
    62221 ttcatatctt aacgctggtt ttttacgaac ttagtgaaat aatgtagaat ataaaattat
    62281 aattttatat tggagcacat gtgttaatga tcaaccccgg gatactaaaa atatagacat
    62341 tattaaactt gcttagcctc gcattgcctt tacctgttaa agatgcatcg agaaaattga
    62401 gtctgaagtt ctcatccttt atctgatttt ccagtcccga aactttggcc ttagcattac
    62461 ctgagatccg tccgttacta tctattttgt aggtaaaaaa atacgaatgg gaaaatttcc
    62521 tcggtgtatc ttcatcagtt aagccaacaa tcgtaaaact accgctacca tcttttttta
    62581 aaatgactga aaggttagca gagtagtata aagaatgatc tgttttagca tttcttgtta
    62641 acgtcgacgt acattcaaga gacgttgtgc taccatcacg agccttccag agagcaaaaa
    62701 aacacacctg tagcaaaaat acattttctt attagtcata taatagcacc tctgaacaaa
    62761 ttgcataagt ttcgccatta gaggatttta tacatcttga taaataccgc tgcccttccc
    62821 caagcaaata atgcatacta tttacatcat atataaaatt tgcacctggc acacagggta
    62881 aatgcttttt cgaaatttca gcggcctcta tttgagattt agcgaggcca atctcgttaa
    62941 gattcgctga aagatataaa tcacaggtgt caatcttacc tgatttaacc aatggataat
    63001 ccgttatact gtaggtatct ttaaaagcca cggcaaacat ttcaataata acaattacta
    63061 ccacaagcga aatcaaaatg cgaaaaagag gaatactcct tttgcgctct actttttcaa
    63121 caacatcttc ttgtaaatga gaatcatcat aagattgttt acttattgtt atgttttcac
    63181 tgatgtaaaa gcctactttg ggtatcgtaa gaatcacatt ttcttcaaca ccaatatttt
    63241 taaaactttt gcgtaacagg gatatatatt ggttcaatgt gttgtttgac agatcaaatg
    63301 aaaatctctc caaaacgcta tcaatgattg agttcctgtc aaccgctcca ccattacttt
    63361 ccagtaacac tgccaaaata caccccgccg tagctggcaa atttatctca tcctgggtat
    63421 ttacattcga gagcgagcat ttgttcggat caaaaataat cgcgtcattg ataatgaaaa
    63481 aacatctccg ctccatgcag gcaacaacac aaaataagat tttgtcattt tagcctattc
    63541 ctggtggaac tgacacaaga aactaaaata caaatataac cctgaaaaag tcatataaaa
    63601 cttatatcca cagaataaaa aaacaaaaca tgtcagtttt atcaatataa agtatcgcaa
    63661 ggcgaaaagt gatgcaagaa tactcctaag taaatgaaag cgcaccacat gaaattatca
    63721 acacatgtaa tttcatcatt aggtgctatc gaaaataact caggagctac ttaagatgaa
    63781 taatgtctta ctcatgagac ccgatgactc agaatttatg atacctgatg actggtcagg
    63841 gctaaacgca aaaggcatta catacgaaca agataataga gtgacagagt cttttctgcc
    63901 ttttaaatct ttttattgtt caccggtgtg cacgacagtt gaactcatct cacaaaataa
    63961 ttgttttgct atgggtatcc gctaccttct ggaaaagaaa agagacttaa ggctaaatca
    64021 ctttaaagcc ttctctcata aatgcgagac aaaacctgac gtgttaatta tgaccgggtg
    64081 caccgtcgct gatacttata gtatattctc acgcattgtt gaagaaaata taatgaaaac
    64141 gatgacacct atcgtcatca ttactgggca tcactcagaa gatctttgtt ctctggcatc
    64201 gaaactggga ggagttaaaa taatatcacc gacagaaaag attgcaaaaa tagaaaacgc
    64261 tatcaatgat gcttttaaca gcaagcctaa aaaaataaga tttttaacag atatactctc
    64321 tcttcaacaa attgagacca tgaaaatggt tgcgcagggt agatccgtta atgagatttc
    64381 agccgcaatg aatattagca tcaagaccgt gtatgcacat aaagttcgtg ctatcaataa
    64441 gctgggaatt aagaaaaaag tttatgaagc gcttttttat aaagcgctaa ttcagttcaa
    64501 ctgatgtaaa aatcaccctc gcttaataaa taatatattc tatgtctttt tttataagtt
    64561 aaaaaaggcg ggatatcaaa tcccgccatg aaattaacta atcatctgca ttattctgcg
    64621 tagatataag cctgggtacg gatggtgtaa gagtctggct caacaacctg ttctttcata
    64681 tgggaaacga cgcgataggc accacctgtt ccagtatttt tcagcatcat ataacgctta
    64741 ccgccaatct cttcggaggc agctgaagga ggatcctgct caagcgttgt cagggaaact
    64801 ttcagtttat ggtccgggtt attgtcacca gaaattactg catccgctga agtactgctc
    64861 tccgcataaa tatcatccgg gaaagttaca gcatagtcgt ggctctgccc atcagatgcg
    64921 ccaacaatac ctgttgccaa aggcgtccat gcagttaacg taccgctata tacccctttc
    64981 actggttgaa tggtattatt aacaacgata ggcttggtca aagtaagatt agctgacgct
    65041 tcagtaccct ggcttactaa agttcccgcc tgagaagcaa aagctgtccc cgccagcatg
    65101 attgctaaca atgttttctt cattatattt tctccatgat tttcatttac actgcaaaat
    65161 gcagtttctt tctttgcatt tacgtgctat cgcgaaaatg cttccaccaa ataatggaaa
    65221 catcttcaca aaaataaatt atgagtttct taatttagaa actataaaca tgtcgccgct
    65281 atctggtata accctgatgc acttttcttc gcaggaagtt ggtattttgc gatacaggat
    65341 tgatctttag aataacccca tttcacatta aactcgcctt ccgcactcaa gccagaaaca
    65401 taagcaatac catcttcacc tacaatgccg gccatatttt cagtgtcttt gctataacta
    65461 atcacagcgc cgaaggggac gggtttaccc tctttctgtt ttaaggttac taatccttta
    65521 tccccgctct tcactgccaa tgtcgcaggc acaacggctc cagctgtcgg tacaaccaat
    65581 tgactagttt gtggtaagtc gtaattctta cctatcgttt tttcatccag aataacatcg
    65641 tctcttcggt atggctgcac tggcagtatt gcatacccac gaaaatcagt actcagtccc
    65701 ggccagccat taacgttaac ccccgttgct ccgggtgcct ctataagggc taatgtccca
    65761 tccacatttt gaccaaatgt aagtccatcc cttgtaataa gtatcccacc gcttaaattt
    65821 gcacctagct gtttcatgtt ttcgctggag ctgtaatggc tactaaactg accatatgat
    65881 ccatttttgc caacttgcac aatgttcatc gtgctcttcg gttgtgactg atgagcgtta
    65941 gttatatttg tcgctacatc ccaccataca ccaccactat tctgacctga taatgaaacc
    66001 tgatagttat taccctgctc tcccgaacga ctcatctgtg aagacgcata aatttgattt
    66061 tcgtcaccca gcgataatgg cactgaaata cccacgctta atttctgatc tactttccat
    66121 ttatgcgatg tcagcaagtc gttttttgcc cagttaatgg agaatgttat gcctctccat
    66181 accgtactat aactcaggct ggtagaccgc gtgtttcctg tgccccggga agaattatac
    66241 ctcgtggtat tgaagttcaa attcccgata ttaccaaggg gctgggataa ggatatcgta
    66301 gagccatcgt gactattctt atctcttttt ctgttccgtt catcctgtgc acttactgct
    66361 tcagtcagtg tggcataacc tgatgcataa cggttcgtgg ttatagac
//
LOCUS       DAJVQH010000007        65613 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJVQH010000007 DAJVQH010000000
VERSION     DAJVQH010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.007"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="OQM87_000540"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="OQM87_000540"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCS1506799.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OQM87_000541"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OQM87_000541"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCS1506800.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OQM87_000542"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OQM87_000542"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCS1506801.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OQM87_000543"
     CDS             complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OQM87_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCS1506802.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OQM87_000544"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OQM87_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCS1506803.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OQM87_000545"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OQM87_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCS1506804.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLHSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="OQM87_000546"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="OQM87_000546"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCS1506805.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7612..10146
                     /gene="mrcB"
                     /locus_tag="OQM87_000547"
     CDS             7612..10146
                     /gene="mrcB"
                     /locus_tag="OQM87_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCS1506806.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLAAIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10366..12609
                     /gene="fhuA"
                     /locus_tag="OQM87_000548"
     CDS             10366..12609
                     /gene="fhuA"
                     /locus_tag="OQM87_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCS1506807.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FGENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12660..13457
                     /gene="fhuC"
                     /locus_tag="OQM87_000549"
     CDS             12660..13457
                     /gene="fhuC"
                     /locus_tag="OQM87_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCS1506808.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13457..14347
                     /gene="fhuD"
                     /locus_tag="OQM87_000550"
     CDS             13457..14347
                     /gene="fhuD"
                     /locus_tag="OQM87_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCS1506809.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14344..16326
                     /gene="fhuB"
                     /locus_tag="OQM87_000551"
     CDS             14344..16326
                     /gene="fhuB"
                     /locus_tag="OQM87_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCS1506810.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16361..17641)
                     /gene="hemL"
                     /locus_tag="OQM87_000552"
     CDS             complement(16361..17641)
                     /gene="hemL"
                     /locus_tag="OQM87_000552"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCS1506811.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLMTEL
                     VPTIDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17866..19287
                     /gene="clcA"
                     /locus_tag="OQM87_000553"
     CDS             17866..19287
                     /gene="clcA"
                     /locus_tag="OQM87_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCS1506812.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGVLVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19311..19376
                     /locus_tag="OQM87_000554"
     CDS             19311..19376
                     /locus_tag="OQM87_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506813.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19369..19713
                     /gene="erpA"
                     /locus_tag="OQM87_000555"
     CDS             19369..19713
                     /gene="erpA"
                     /locus_tag="OQM87_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCS1506814.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19760..20383)
                     /locus_tag="OQM87_000556"
     CDS             complement(19760..20383)
                     /locus_tag="OQM87_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCS1506815.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20421..21221)
                     /gene="btuF"
                     /locus_tag="OQM87_000557"
     CDS             complement(20421..21221)
                     /gene="btuF"
                     /locus_tag="OQM87_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCS1506816.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21214..21912)
                     /gene="mtnN"
                     /locus_tag="OQM87_000558"
     CDS             complement(21214..21912)
                     /gene="mtnN"
                     /locus_tag="OQM87_000558"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCS1506817.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21996..23513
                     /gene="dgt"
                     /locus_tag="OQM87_000559"
     CDS             21996..23513
                     /gene="dgt"
                     /locus_tag="OQM87_000559"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCS1506818.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23643..25067
                     /gene="degP"
                     /locus_tag="OQM87_000560"
     CDS             23643..25067
                     /gene="degP"
                     /locus_tag="OQM87_000560"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCS1506819.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25222..26378
                     /gene="cdaR"
                     /locus_tag="OQM87_000561"
                     /pseudo
     CDS             25222..26378
                     /gene="cdaR"
                     /locus_tag="OQM87_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26467..26853)
                     /locus_tag="OQM87_000562"
     CDS             complement(26467..26853)
                     /locus_tag="OQM87_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCS1506820.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27167..27991)
                     /gene="dapD"
                     /locus_tag="OQM87_000563"
     CDS             complement(27167..27991)
                     /gene="dapD"
                     /locus_tag="OQM87_000563"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCS1506821.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28022..30694)
                     /gene="glnD"
                     /locus_tag="OQM87_000564"
     CDS             complement(28022..30694)
                     /gene="glnD"
                     /locus_tag="OQM87_000564"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCS1506822.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30756..31550)
                     /gene="map"
                     /locus_tag="OQM87_000565"
     CDS             complement(30756..31550)
                     /gene="map"
                     /locus_tag="OQM87_000565"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCS1506823.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31918..32643
                     /gene="rpsB"
                     /locus_tag="OQM87_000566"
     CDS             31918..32643
                     /gene="rpsB"
                     /locus_tag="OQM87_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCS1506824.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32901..33752
                     /gene="tsf"
                     /locus_tag="OQM87_000567"
     CDS             32901..33752
                     /gene="tsf"
                     /locus_tag="OQM87_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCS1506825.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33899..34624
                     /gene="pyrH"
                     /locus_tag="OQM87_000568"
     CDS             33899..34624
                     /gene="pyrH"
                     /locus_tag="OQM87_000568"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCS1506826.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34916..35473
                     /gene="frr"
                     /locus_tag="OQM87_000569"
     CDS             34916..35473
                     /gene="frr"
                     /locus_tag="OQM87_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCS1506827.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35565..36761
                     /gene="ispC"
                     /locus_tag="OQM87_000570"
     CDS             35565..36761
                     /gene="ispC"
                     /locus_tag="OQM87_000570"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCS1506828.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36950..37708
                     /gene="ispU"
                     /locus_tag="OQM87_000571"
                     /gene_synonym="uppS"
     CDS             36950..37708
                     /gene="ispU"
                     /locus_tag="OQM87_000571"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCS1506829.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37721..38578
                     /gene="cdsA"
                     /locus_tag="OQM87_000572"
     CDS             37721..38578
                     /gene="cdsA"
                     /locus_tag="OQM87_000572"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1506830.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWCADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38590..39942
                     /gene="rseP"
                     /locus_tag="OQM87_000573"
     CDS             38590..39942
                     /gene="rseP"
                     /locus_tag="OQM87_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCS1506831.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39972..42404
                     /gene="bamA"
                     /locus_tag="OQM87_000574"
     CDS             39972..42404
                     /gene="bamA"
                     /locus_tag="OQM87_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCS1506832.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42526..43011
                     /gene="skp"
                     /locus_tag="OQM87_000575"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42526..43011
                     /gene="skp"
                     /locus_tag="OQM87_000575"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCS1506833.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43015..44040
                     /gene="lpxD"
                     /locus_tag="OQM87_000576"
     CDS             43015..44040
                     /gene="lpxD"
                     /locus_tag="OQM87_000576"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCS1506834.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44145..44600
                     /gene="fabZ"
                     /locus_tag="OQM87_000577"
     CDS             44145..44600
                     /gene="fabZ"
                     /locus_tag="OQM87_000577"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCS1506835.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44604..45392
                     /gene="lpxA"
                     /locus_tag="OQM87_000578"
     CDS             44604..45392
                     /gene="lpxA"
                     /locus_tag="OQM87_000578"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCS1506836.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45392..46540
                     /gene="lpxB"
                     /locus_tag="OQM87_000579"
     CDS             45392..46540
                     /gene="lpxB"
                     /locus_tag="OQM87_000579"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCS1506837.1"
                     /translation="MTDQRPLTIALVAGEISGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46537..47133
                     /gene="rnhB"
                     /locus_tag="OQM87_000580"
     CDS             46537..47133
                     /gene="rnhB"
                     /locus_tag="OQM87_000580"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCS1506838.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47170..50652
                     /gene="dnaE"
                     /locus_tag="OQM87_000581"
     CDS             47170..50652
                     /gene="dnaE"
                     /locus_tag="OQM87_000581"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCS1506839.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50665..51624
                     /gene="accA"
                     /locus_tag="OQM87_000582"
     CDS             50665..51624
                     /gene="accA"
                     /locus_tag="OQM87_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCS1506840.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51723..53864
                     /gene="ldcC"
                     /locus_tag="OQM87_000583"
     CDS             51723..53864
                     /gene="ldcC"
                     /locus_tag="OQM87_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCS1506841.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53921..54310
                     /locus_tag="OQM87_000584"
     CDS             53921..54310
                     /locus_tag="OQM87_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1506842.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54375..55673
                     /gene="tilS"
                     /locus_tag="OQM87_000585"
     CDS             54375..55673
                     /gene="tilS"
                     /locus_tag="OQM87_000585"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCS1506843.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55722..55982)
                     /gene="rof"
                     /locus_tag="OQM87_000586"
     CDS             complement(55722..55982)
                     /gene="rof"
                     /locus_tag="OQM87_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCS1506844.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55969..56169)
                     /locus_tag="OQM87_000587"
     CDS             complement(55969..56169)
                     /locus_tag="OQM87_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCS1506845.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56335..56868
                     /locus_tag="OQM87_000588"
     CDS             56335..56868
                     /locus_tag="OQM87_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCS1506846.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAW"
     gene            56877..57299
                     /gene="arfB"
                     /locus_tag="OQM87_000589"
     CDS             56877..57299
                     /gene="arfB"
                     /locus_tag="OQM87_000589"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1506847.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57313..58023
                     /gene="nlpE"
                     /locus_tag="OQM87_000590"
                     /gene_synonym="cutF"
     CDS             57313..58023
                     /gene="nlpE"
                     /locus_tag="OQM87_000590"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCS1506848.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58178..59002)
                     /locus_tag="OQM87_000591"
     CDS             complement(58178..59002)
                     /locus_tag="OQM87_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCS1506849.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRNIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59055..60773)
                     /gene="proS"
                     /locus_tag="OQM87_000592"
     CDS             complement(59055..60773)
                     /gene="proS"
                     /locus_tag="OQM87_000592"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCS1506850.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60884..61591)
                     /gene="tsaA"
                     /locus_tag="OQM87_000593"
     CDS             complement(60884..61591)
                     /gene="tsaA"
                     /locus_tag="OQM87_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCS1506851.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61588..61992)
                     /gene="rcsF"
                     /locus_tag="OQM87_000594"
     CDS             complement(61588..61992)
                     /gene="rcsF"
                     /locus_tag="OQM87_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCS1506852.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62110..62925)
                     /gene="metQ"
                     /locus_tag="OQM87_000595"
     CDS             complement(62110..62925)
                     /gene="metQ"
                     /locus_tag="OQM87_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCS1506853.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62965..63618)
                     /gene="metI"
                     /locus_tag="OQM87_000596"
     CDS             complement(62965..63618)
                     /gene="metI"
                     /locus_tag="OQM87_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCS1506854.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63611..64642)
                     /gene="metN"
                     /locus_tag="OQM87_000597"
     CDS             complement(63611..64642)
                     /gene="metN"
                     /locus_tag="OQM87_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCS1506855.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64830..65405
                     /gene="gmhB"
                     /locus_tag="OQM87_000598"
     CDS             64830..65405
                     /gene="gmhB"
                     /locus_tag="OQM87_000598"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCS1506856.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15511 a        16250 c        18081 g        15771 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag
     2341 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga ccctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacatga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tattcgcgca cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaatgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaacctgg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcgggtt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cgctgtatgg cgcgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccga acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaacccta accctctccc
     7501 tcaagggaga ggggaccgat cgagcacaaa ttttgagaga tatcttcttc tgtcttgtaa
     7561 cagaagaaca gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag catggccggg
     7621 aatgaccgcg agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta
     7681 agccgtcgtc gttacgaaga tgacgatgat tacgacgatt atgatgacta tgaggatgaa
     7741 gaaccgatgc cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg tggcaaacgc
     7801 ggctggctat ggctactgct aaaactggct atcgtttttg ccgtgctgat cgccatttac
     7861 ggcgtttatc tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg gcaactgccc
     7921 gcggcagttt atggccgaat ggtcaacctt gaaccagata tgaccatcag caagaacgaa
     7981 atggtgaagc tgctggaggc aacacagtat cgtcaggtgt cgaaaatgac ccgtcctggc
     8041 gaatttaccg tgcaggccaa cagcattgag atgatccgcc gtccgtttga tttcccggac
     8101 agtaaagaag ggcaggtgcg tgcgcgtctg acctttgatg gtgatcatct ggcggccatc
     8161 gtcaatatgg agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg tctgatcacc
     8221 atgatctcct cgccaaacgg tgagcagcgt ttgtttgtgc cgcgcagtgg tttccctgat
     8281 ttgctggtgg atactttgct ggcgacagaa gaccgtcatt tttacgagca tgatggaatc
     8341 agtctctact ccattgggcg tgcggtgctg gcaaacctga ccgcaggtcg cacggtgcag
     8401 ggtgcgagta cgctgacgca acagctggtg aaaaacctgt tcctctccag cgagcgttct
     8461 tactggcgta aagcgaacga agcttacatg gcgctgatca tggacgcgcg ttacagcaaa
     8521 gaccgtattc ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg cgacaacgaa
     8581 atccgcggct tcccgctggc aagcttgtat tactttggtc gcccggtaga agagctaagc
     8641 ctcgaccagc aggcgctgtt agtcggtatg gtgaaagggg cgtccatcta caacccgtgg
     8701 cgtaacccga aactggcgct ggagcgacgt aatctggtgc tgcgtctgct gcaacagcaa
     8761 cagattattg atcaagaact ctatgacatg ttgagtgccc gtccgctggg ggttcagccg
     8821 cgtggtgggg tgatctctcc tcagccagcc tttatgcagt tggtgcgtca ggagttgcag
     8881 gcaaaactgg gcgataaggt aaaagatctc tccggcgtga agatcttcac tacctttgac
     8941 tcggtggccc aggacgcggc agaaaaagcc gccgtggaag gcattccggc actgaagaaa
     9001 cagcgtaagt tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt tagtggtgaa
     9061 gttcgtgcga tggtcggagg ttctgagccg cagtttgcgg gctacaaccg tgcgatgcag
     9121 gcgcgtcgtt cgattggttc ccttgcaaaa ccagcgactt atctgacggc tttaagccag
     9181 ccgaaaatct atcgcctgaa cacgtggatt gccgatgcgc caatcgcgct gcgtcagccg
     9241 aatggccagg tctggtcacc gcagaacgat gaccgtcgtt atagcgaaag cggcagagtg
     9301 atgctggtgg atgcgttgac acgttcgatg aacgtgccga cggtaaatct ggggatggcg
     9361 ctggggctgc ctgcggttac ggagacctgg atcaaactgg gcgtaccgaa agatcagttg
     9421 catccggttc cggcaatgct gctgggggcg ttgaacttaa cgccaatcga agtggcgcag
     9481 gcattccaga ccatcgccag cggtggtaac cgtgcaccgc tttctgcact gcgttcggta
     9541 atcgcggaag atggtaaggt gctgtatcag agcttcccgc aggcagaacg cgctgttccg
     9601 gcgcaggcgg cgtatctgac actatggacc atgcagcagg tggtacaacg tggtacgggt
     9661 cgtcagcttg gggcgaaata cccgaacctg catctggcag ggaaaacagg gactaccaac
     9721 aataacgtag atacctggtt tgcaggcatt gacggcagca cggtgaccat cacctgggta
     9781 ggccgtgata acaaccagcc gaccaaactg tatggagcca gcggggcaat gtcgatttat
     9841 cagcgttatc tggctaacca gacgccaacg ccgctgaatc ttgttccgcc agaagatatt
     9901 gcagatatgg gcgtggacta cgacggcaac tttgtttgca gcggtggcat gcgtgtcttg
     9961 ccggtctgga ccagcgatcc gcaatcgctg tgccagcaga gcgagatgca gcagcagccg
    10021 tcaggcaatc cgtttgatca gtcttctcag ccgcagcaac agccgcaaca gcaacctgct
    10081 cagcaagagc agaaagacag cgacggtgta gccggttgga tcaaggatat gtttggtagt
    10141 aattaacatc taagcgtgaa ataccggatg gcgagttgcc atccggtaaa ataacatccc
    10201 atctaagata ttaacccttt cttttcatct ggttgtttat taacccttca ggaacgctca
    10261 gattgcgtac cgcttgcgaa cccgccagcg tttcgaatat tatcttatct ttataataat
    10321 cattctcgtt tacgttatca ttcactttac atcagagata taccaatggc gcgttccaaa
    10381 actgctcagc caaaacactc actgcgtaaa atcgcagttg tagtagccac agcggttagc
    10441 ggcatgtctg tttatgcaca ggcagcggtt gaaccgaaag aagacactat caccgttacc
    10501 gctgcacctg cgccgcaaga aagcgcatgg gggcctgctg caactattgc ggcgcggcag
    10561 tcagctaccg gcactaaaac cgatacgccg attcaaaaag tgccacagtc tatttctgtt
    10621 gtgaccgccg aagagatggc gctgcatcag ccgaagtcgg taaaagaagc gcttagctac
    10681 acgccgggtg tctctgttgg tacgcgtggc gcatccaaca cctatgacca cctgatcatt
    10741 cgcggttttg cggcagaagg ccaaagccaa aataactatc tgaatggcct gaagttgcag
    10801 ggcaacttct ataacgatgc ggtcattgac ccgtatatgc tggaacgcgc tgaaattatg
    10861 cgtggcccgg tttccgtgct ttacggtaaa agcagtcctg gcggtctgtt gaatatggtc
    10921 agcaagcgtc cgaccaccga accgctgaaa gaagttcagt ttaaagccgg tactgacagc
    10981 ctgttccaga ctggttttga ctttagcgat gcgttggatg atgacggtgt ttactcttat
    11041 cgcctgaccg gtcttgcgcg ttctgccaat gcccagcaga aagggtcaga agagcagcgt
    11101 tatgctattg caccggcgtt cacctggcgt ccggatgata aaaccaattt caccttcctt
    11161 tcttacttcc agaacgagcc ggaaaccggt tattacggct ggttgccgaa agagggaacc
    11221 gttgagccgc tgccgaacgg taagcgtctg ccgacagact ttaatgaagg ggcgaagaac
    11281 aacacctatt ctcgtaatga gaagatggtc ggctacagct tcgatcacga atttaacgac
    11341 acctttactg tgcgtcagaa cctgcgcttt ggtgaaaaca aaacctcgca aaacagcgtt
    11401 tatggttacg gcgtctgctc cgatccggcg aatgcttaca gcaaacagtg tgcggcatta
    11461 gcgccagcgg ataaaggcca ttatctggca cgtaaatacg tcgttgatga tgagaagctg
    11521 caaaacttct ccgttgatac ccagttgcag agcaagtttg ccactggcga tatcgaccac
    11581 accctgctga ccggtgtcga ctttatgcgt atgcgtaatg acatcaacgc ctggtttggt
    11641 tacgacgact ctgtgccact gctcaatctg tacaatccgg tgaatcccga tttcgacttc
    11701 aatgccaaag atccggcaaa ctccggccct taccgcattc tgaataaaca gaaacaaacg
    11761 ggcgtttatg ttcaggatca ggcgcagtgg gataaagtgc tggtcaccct aggcggtcgt
    11821 tatgactggg cagatcaaga atctcttaac cgcgttgctg ggacgaccga taaacgtgat
    11881 gacaaacagt ttacctggcg tggtggtgtt aactacctgt ttgataatgg tgtaacacct
    11941 tacttcagct atagcgaatc gtttgaacct tcttcgcaag ttgggaagga tggtaatatt
    12001 ttcgcaccgt ctaaaggtaa gcagtatgaa gtcggcgtga aatatgtacc ggaagatcgt
    12061 ccgattgtag ttactggtgc cgtgtataat ctcactaaaa ccaacaacct gatggcggac
    12121 cctgagggtt ccttcttctc ggttgaaggt ggcgagatcc gcgcacgtgg cgtagaaatc
    12181 gaagcgaaag cggcgctgtc ggcgagtgtt aacgtagtcg gttcttatac ttacaccgat
    12241 gcggaataca ccaccgatac tacctataaa ggcaatacgc ctgcacaggt gccaaaacac
    12301 atggcttcgc tgtgggctga ctataccttc tttgacggtc cgctttcagg tttgacgctg
    12361 ggcaccggtg gtcgttatac tggctccagc tatggtgatc cggctaactc ctttaaagtg
    12421 ggaagttata cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt cggcatggcg
    12481 ggctccaacg tggcgctgca tgttaacaac ctgttcgatc gtgaatacgt cgccagctgc
    12541 tttaacactt atggctgctt ctggggcgca gaacgtcagg tcgttgcaac cgcaaccttt
    12601 cgtttctaat ttctcttttg gggcacggat ttccgtgccc atttcacaag ttggctgtta
    12661 tgcaggaata cacgaatcat tccgatacca cttttgcact gcgtaatatc tcctttcgtg
    12721 tgcccgggcg cacgcttttg catccgctgt cgttaacctt tcctgccggg aaagtgaccg
    12781 gtctgattgg tcacaacggt tctggtaaat ccactctgct caaaatgctt ggtcgtcatc
    12841 agcctccgtc agaaggggag attcttcttg atgcccagcc gctggaaagc tggagcagca
    12901 aagcgtttgc ccgcaaagtg gcttatttgc cgcagcagct tcctccggca gaagggatga
    12961 ccgtgcgtga actggtggcg attggtcgtt acccgtggca tggcgcgctg gggcgctttg
    13021 gtgcggcaga tcgcgaaaag gtcgaggaag ctatctcgct ggttggctta aaaccgctgg
    13081 cgcatcggct ggtcgatagt ctctctggcg gcgaacgtca gcgggcgtgg atcgccatgc
    13141 tggtggcgca ggatagccgt tgtctgttgc tcgacgaacc gacttcggcg ctggatatcg
    13201 cccaccaggt tgatgtgctg gcgctggtgc accgtttaag tcaggaacgt ggcctgacgg
    13261 tcattgccgt gttgcacgat atcaatatgg cggcacgcta ctgtgattat ctggtcgccc
    13321 tgcgcagtgg tgaaatgatt gctcagggaa cgcctgcgga aattatgcgc ggcgaaaccc
    13381 tcgaaatgat ttatggcatc ccgatgggta ttttgccgca tccggcgggt gctgcacctg
    13441 tgagttttgt ttattgatga gcggcttacc tcttatttcg cgccgtcgac tgttaacggc
    13501 gatggcgctt tctccgttgt tatggcagat gaataccgcc cacgcggcgg ctattgaccc
    13561 caatcgtatt gtggcgctgg agtggttgcc ggtggaatta ctgctggcgc tcggcatcgt
    13621 gccttacggc gtggcggata ccatcaacta tcgcctgtgg gtcagcgaac caccattgcc
    13681 ggactcagtg attgacgtcg gtttgcgtac agaacctaac cttgaactgc tgaccgaaat
    13741 gaaaccgtcg tttatggtct ggtcggcagg atatggccct tcatcagaaa tgctggcacg
    13801 tattgcgccg ggtcgcggat ttaacttcag tgacggcaaa catccgttgg cgatggcgcg
    13861 taaatcgctg acggaaatgg cagatttact taacctgcaa agcgcagcgg aaacgcattt
    13921 agcgcaatac gaagacttta tccgcagcat gaaaccccgc tttgtgaagc gtggtgcacg
    13981 cccgttactg ctgacgacgc ttatcgatcc gcgccatatg ctggtcttcg gtccaaacag
    14041 cttgttccag gaaattctcg atgagtacgg catcccaaat gcctggcaag gggaaaccaa
    14101 cttctggggc agtaccgccg tcagtatcga tcgtctggca gcgtataaag acgttgatgt
    14161 gctctgtttt gatcacgaca acagcaaaga catggatgcg ctaatggcaa ccccgctgtg
    14221 gcaggccatg ccgtttgtcc gtacagggcg ctttcagcgc gtacccgcag tctggtttta
    14281 tggtgcaacg ctttcggcaa tgcactttgt gcgtgttctg gataacgcca tcggaggtaa
    14341 agcgtgagta aacgaattgc gcttttcccg gcgttattgc tggcgctgtt agtgattgtc
    14401 gctacggcgc tcacctggat gaacttctca caggcgctgc cgcgcagtca gtgggcgcag
    14461 gccgcctggt cgccggatat tgacgtcatc gagcagatga tttttcacta cagcttgttg
    14521 ccgcgtctgg cgatttcgct gctggtgggc gcgggcctgg ggctggtggg cgtgctgttt
    14581 cagcaagtac tgcgtaaccc gctggcggaa ccgacgacgt tgggcgttgc aacaggcgcg
    14641 caactgggga ttaccgtcac cacgctctgg gcgatcccta gcgcgatggc gagccagttt
    14701 gctgcgctgg caggggcttg cgttgttggc ttaattgtct ttggcgtcgc gtgggggaaa
    14761 cggctgtcgc cggtaacgct gattctcgcg gggttggtag tgagccttta ttgcggcgca
    14821 atcaatcagt tactggttat cttccatcat gaccagttgc aaagcatgtt tctgtggagc
    14881 actggaacgc tgacgcaaac cgactggggc ggtgttgagc gtttatggcc gcagttgctg
    14941 ggcggtgtga tgctgacgtt gctgctactt cgtccgttaa ccctgatggg gcttgatgat
    15001 ggcgtggcgc gcaatctcgg gctggccttg tcgcttgcgc gtctggcggc gctgtcgctg
    15061 gcgattgtca tcagtgcgct gctggtgaac gctgtgggga ttatcggctt tatcgggttg
    15121 ttcgcgccgc tgctggcaaa aatgctgggg gcgcggcgtc tgctgccacg actgatgctg
    15181 gcgtcgttga ttggtgcgct gatcctctgg ctttccgatc aaatcatcct ctggctgact
    15241 cgcgtgtgga tggaagtgtc caccggttcg gtcactgcgt tgatcggtgc gccgctgcta
    15301 ctgtggctgc tgccgcgttt acgcagcatt agcgcgccgg atatgaaggt taacgatcgt
    15361 gtcgcgactg aacgccaaca tgtgctggcg tttgccctcg cgggcggcgt gctgttgttg
    15421 atggctgtgg tggtggcgct gtcgtttggt cgtgatgcgc acggctggac gtgggcgagc
    15481 ggggcgttgc tcgaggattt aatgccctgg cgctggccgc gaattatggc ggcgctgttt
    15541 gcgggcgtca tgctggcggt agcgggctgt attattcagc gactgaccgg caacccgatg
    15601 gcaagcccgg aagtgctggg gattagctcc ggcgcggcgt ttggtgtggt gttaatgctg
    15661 tttctggtac cgggtaatgc ctttggctgg ttgttacccg caggcagtct cggggcggcg
    15721 gtgatgctgt tgatcattat gatcgccgcc ggacgcggtg gattttcccc acaccgcatg
    15781 ttactggcgg ggatggcgtt aagtaccgcg ttcaccatgc ttttgatgat gttgcaggca
    15841 agtggtgacc cgcgaatggc gcaagtgctg acctggattt ccggttcgac ctacaacgcg
    15901 accgatgctc aggtctggcg caccggaatt gtgatggtga ttttgctggc gattactccg
    15961 ctgtgccgcc gctggctgac cattttaccg ctgggtggtg ataccgcgcg ggcggtaggg
    16021 atggcgctga cgccgacgcg aattgcgctg ctgctgttag cggcttgcct gacggcgacc
    16081 gcgacgatga ctattggacc gttgagtttt gttggtttaa tggcaccgca tattgcgcgg
    16141 atgatgggct ttcgacggac gatgccacat atcgtaattt cggcgctggt gggtggttta
    16201 ctgctggtgt tcgctgactg gtgtgggcgg atggtgctgt ttccattcca gatcccggcg
    16261 gggctgctgt cgacctttat cggtgcgcca tattttatct atttgctgag aaagcagagc
    16321 cgttaataga atgcctcgcc cggtgggcga ggcaaaatta tcacaacttc gcgaacaccc
    16381 gacgtgcagc atcgatggtg ttattgatat cttccatgct gtgcgccaca gacataaagc
    16441 ccgcttcaaa cgctgacggt gccaggtaaa caccttcatc cagcatcata tggaagaaac
    16501 gcttaaaacg ctcgacgtca caggccatca catcctgata gcacgtcacg gactcggcgt
    16561 cggtaaagaa gataccgaac ataccaccga cgtggttaac gaccagcgga attcccgctt
    16621 cttctgccgc ttccagcaga ccttctgcca gacgtgttgt cagctcatcc agcgtttcgt
    16681 gaatgcccgg ctgcgcgact tcattcagac aggcgaaacc cgctgccatc gcaatcgggt
    16741 taccggaaag cgtacccgcc tgatagaccg gacctgtagg ggccagcgca tccattacat
    16801 cacgacgacc accgaatgcg cctaccggca ttccaccgcc gatgattttt ccgaggcagg
    16861 ttaaatctgg cactacgccg aaataatcct gtgcgccagc tagcgctacg cggaaaccgg
    16921 tcatcacttc atcgatgatc agcaacgcgc caaattcgtc gcacagcgca cgcagacctg
    16981 gcaggaactc tggcagcggc ggaacacagt tcatattgcc tgccaccggc tcgacgataa
    17041 tacaggcaat ctcttgcggg tattgctcaa acgcggcgcg tacagaggcc agatcgttat
    17101 aagtacaggt taaggtatgt ttggcgaaat ctgccggaac gcccggcgag tttggctggc
    17161 ctaacgtgag tgcgccagaa ccggctttca ccagcaggca gtcagcgtga ccgtggtaac
    17221 aaccttcaaa tttaatgatt ttgtcgcgac cggtaaaacc acgggccaag cggatggcgc
    17281 tcatcgtcgc ctcggtgccg gagttcacca tgcgcaccat atctatggtc gggaccagtt
    17341 cggtcatcag ttgcgccatt ttcacttcca tttcggtcgg tgcaccaaag cttaaaccac
    17401 gctcggcggc ttcaatcacg gcattgcgga tcgccggatg gttatggccc agcaccatcg
    17461 gcccccagga accgacataa tcgatatagg ctttgccatc aacatcgtac agataagcgc
    17521 cgtccgcttt ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg cgaacagggg
    17581 agttcacacc gccagggatc agctcgcgcg ctgcgctgta aagattttca gacttactca
    17641 tggagggttc ctgattcgta gaaaaagtga atggctgcta ttctatgtta ttcataacaa
    17701 gttaaatact cgtcaaacat caggctgctt gtactggtgc aatcctgatt tcttagagta
    17761 taaaagtttt gtgcatttga aacattacgc tttgcaaagg attttcatgg aacgtgcgag
    17821 taaaatgccg tcatcttatt tgtatgacca ataagtgatc attggatgaa aactgatact
    17881 ccctctttag aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat tcgccaactt
    17941 cttgagcgcg ataaaacccc gttagccatt ttgtttatgg cggcagtcgt cggcacgctt
    18001 atcgggctgg ctgcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa ccaacgtatg
    18061 ggagtgctgg tacatactgc tgataattat ccgctactgt taaccgtcgc ttttctctgt
    18121 tcggcggtgc tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc cgaagcaggg
    18181 ggatcgggga tcccggaaat tgaaggggcg ctggaagatc aacgtcctgt tcgctggtgg
    18241 cgtgtattgc cggtgaaatt ctttggcggg ctggggacac tcggcggagg catggtgcta
    18301 gggcgcgaag gaccaaccgt gcagatcggc ggcaatatcg gtcgcatggt gcttgatatt
    18361 ttccgcctga aaggtgatga agctcgccat acgctgctgg caaccggtgc tgctgcgggg
    18421 ctggctgcgg cctttaacgc gccgctggcg ggtattttgt ttattatcga agagatgcgt
    18481 ccgcagtttc gctatacgtt aatttcgatt aaagcggtat ttattggcgt cattatgtcg
    18541 accattatgt accggatttt taatcatgaa gttgcgttga ttgacgtcgg taaactttct
    18601 gatgcgccgc ttaatacgct gtggctttat ctgatcctcg gtattatttt tggcattttc
    18661 ggccctattt ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg tgtgcacggc
    18721 ggcaatatta ccaaatgggt actgatgggg ggggcgattg gcggtctgtg tggattgctg
    18781 gggtttgtgg caccagcaac gtcgggcggc ggttttaacc tgattcctat cgctaccgcg
    18841 gggaatttca gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat taccacctta
    18901 ctctgcttct cttccggcgc gccgggcggt atttttgccc cgatgctggc gctgggtact
    18961 gtgctgggaa ccgctttcgg aatggttgcc gttgagctgt ttccgcaata tcacctggag
    19021 gcggggacgt ttgctattgc cggaatgggg gcattactgg cggcatctat tcgcgcgccg
    19081 ttaacgggga tcattctggt tctggagatg accgataact accagctcat tttgccaatg
    19141 attattaccg gtcttggcgc aacactatta gcgcaattta ccggcgggaa acctctatac
    19201 tcggcgattc ttgcgcgcac gctggcaaaa caggaagctg agcaactggc gcgaagcaag
    19261 gccgcatcag ccagcgagaa tacttgaacg aaataccagg gtattagata atggcgatta
    19321 ttattgggtt agaatttgcc cgattgccga tgtcgtttgg agcaaaatat gagtgatgac
    19381 gtagcactgc cgctggagtt taccgacgca gcagccaaca aagttaaaag cctgatcgct
    19441 gacgaagata acccgaatct gaaattacgc gtgtatatca ccggtggcgg ttgcagcggc
    19501 ttccagtatg gtttcacctt tgatgatcag gtgaacgaag gcgatatgac catcgaaaaa
    19561 cagggcgttg gcctggtggt tgatccgatg agcctgcaat atctggtcgg cggttccgtt
    19621 gactacaccg aaggactgga aggttctcgt ttcatcgtga ccaacccgaa cgcgaaaagc
    19681 acctgcggtt gcggttcttc ctttagcatc taatctgttg ttcatgtgcc ggataaacta
    19741 tccggcacat cttctctctt caacgcccat tctcatccag cgcaaacgtc ggtagcttaa
    19801 gatgccaacg aatagccgcc agccgaatca atagcgtcac gaccatgccc atcatactgg
    19861 ctgtttccag tggtacggaa aatgtgtaat aagccgtagc gtggacaata ccgccgataa
    19921 tacaggcagt tgcgtagatt tctgtacgta aaatcatggg gatttcgcgg gccagtacat
    19981 cacgaatgat cccgccgcca acgccagtaa tgacgcccat acaaaccgcg attaacggac
    20041 cggcttccgc attaaaggct ttattcacgc caatgccgac aaacaccgcc agaccaacgg
    20101 cgtccagcac cggcaacatc cattttggta agcgtcttgg ctggcgcacc agcacgatgg
    20161 tcagcatgct ggtgaccatg gcaacgacca gatcggtggg atctttcacc caaaataccg
    20221 ggccgtgatc cagcgccatg tcgcgaattg ttccgtcgcc tactgcggta accacgccca
    20281 gtaccagaac accaaaaggg tccatacgca attttccggc taacaaaacg ccggagatgg
    20341 caaataccgc tgtgccgact atatccagcc aatagacgag cattgttcaa tccccactga
    20401 gcacctgaaa aggtcaggca ctaatctacc tgtgaaagcg cattacagag ctgttgtgct
    20461 gcgaggataa tacgtgggct tgcacgttca aaccagtcac tcgtgagagg aataacggga
    20521 attttgagct gttcacccca gtattgtttg attttaggaa tttggtccgg tccgcctgta
    20581 atgacaatcg cctgtggcga gcgtgctaac acctgttcgc ggctaacttg cggccaggga
    20641 acccggctgt ctttaaagat gttttctccg ccacaaactt cgagtacctg gttctgaatc
    20701 gactcttttc cactggtaaa tggcggatta atgccgaatt gcagaaaaac acgtttttta
    20761 ggtttatcag catattgcgc tttcaattgc acgtactgat ccagcaggga ttgcgcggct
    20821 tgttcggcct tgtctggttg cggactccag ggggccagtt gacgtaacgc attggcaatt
    20881 tgttcaatgc ttgtcgcatc gacccacatc acttttattc ccagcgaagc cagctggtca
    20941 acctgccgct cggcattacc tccacgccag gcaatcacca gatcgggttt cagcgcgaca
    21001 atgcgttcca gattcatccc ctgccaggtg gaaacctgct caatcttttg cgcttgtgga
    21061 ggatagtcgg aatagctgct gaccccaacc ggcgtgatcc cggcggcaaa ggcaagttca
    21121 gtgttggcgg gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag tggcgcgaga
    21181 aaagacaggg cgaccagcgc cctgaacagt gatttagcca tgtgccagtt tctgcaccag
    21241 tgactcaacc atcaggctgg actgtttagc ggcaacagcc aggaactcat cgaagctaag
    21301 atgagactgt tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg ggacgttgaa
    21361 attgtggcag acatgggcga ttgccgtcgc ttccatctct acagcaatgg cctgtgggaa
    21421 gttgtggcgg attttcgcca gaccaacaga accgttgatg aaagcgtcgc cgctaacaat
    21481 caggccacgt acagcgttaa gattcagttc ggcaatgcag gcctcagcgg cagcgatcag
    21541 tttatcgtca gctttaaagc ctgtcggaca gcctggtaac tgaccgtatt cataaccaaa
    21601 tgccgtgaca tccgcgtcgt gataacgcgc ttcgtccgag acaacgatat cgcccacttt
    21661 cagcgttggt gccaggccac cggcagaacc ggtgttaata atcacatctg gcttgcagtg
    21721 ttccaacagc agagtggcac ccagcgccgc agcgacttta ccgatgcccg atttcagaag
    21781 cgcaacctcg gttccattca gttggccggt atagatttcg caaccgccga gactgatagt
    21841 ttgacggttt tcgattttgt cacgcagcag cgtaacttct tcttccattg caccaatgat
    21901 gccgattttc atagatttac tcgcgataag cccgatttga aggcatagtt taccatgcgc
    21961 ttacggggaa gcgtatttct cacgcgggag aggacatggc acagattgat ttccgaaaaa
    22021 aaataaactg gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa catgagatcc
    22081 tgcggatctt cgagagcgat cgcgggcgta tcatcaactc tccggcaatt cgtcgtctgc
    22141 aacaaaagac ccaggttttt ccactggagc gcaatgccgc cgtgcgcacg cgtcttaccc
    22201 actcgatgga agtccagcag gtgggacgct acatcgccaa agaaatttta agccgtctga
    22261 aagagcttaa attgctggaa gcatacggcc tggatgaact gaccggcccc tttgaaagca
    22321 ttgttgagat gtcatgcctg atgcacgata tcggcaatcc gccgtttggt cattttggcg
    22381 aagcagcgat aaatgactgg tttcgccaac gtttgcaccc ggaagatgcc gaaagccagc
    22441 ctctgactga cgatcgctgc agcgtggcgg cactacgttt acgggacggg gaagaaccgc
    22501 ttaacgagct gcggcgcaag attcgtcagg acttatgtca ttttgagggg aatgcacaag
    22561 gcattcgtct ggtgcataca ttgatgcgga tgaatctcac ttgggcacag gttggcggta
    22621 ttttaaaata tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca catcactatt
    22681 taatgaaaaa gccgggttat tatctttctg aagaagccta tattgcccgg ttgcgtaaag
    22741 aacttaattt ggcgctttac agtcgttttc cattaacgtg gattatggaa gctgccgacg
    22801 acatctccta ttgtgtggca gaccttgaag atgcggtaga gaaaagaata tttaccgttg
    22861 agcagcttta tcatcatttg cacgaagcgt ggggccagca tgagaaaggt tcgctctttt
    22921 cgctggtggt tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc cgcagtacgg
    22981 aagatcagtt ttttatgtat ttacgggtaa acaccctaaa caaactggta ccctacgcgg
    23041 cacaacgatt tattgataat ctgcctgcga ttttcgccgg aacgtttaat catgcattat
    23101 tggaagatgc cagcgaatgc agcgatcttc ttaagctata taaaaatgtc gctgtaaaac
    23161 atgtatttag ccatccggat gtcgagcagc ttgaattgca gggctatcgg gtcattagcg
    23221 gattattaga gatttatcgt cctttattaa gcctgtcgtt atcagacttt actgaactgg
    23281 tagaaaaaga acgggtgaaa cgtttcccta ttgagtcgcg cttattccac aaactctcga
    23341 cgcgccatcg gctggcctat gtcgaggctg tcagtaaatt accgtcagat tctcctgagt
    23401 ttccgctatg ggaatattat taccgttgcc gcctgctgca ggattatatc agcggtatga
    23461 ccgacctcta tgcgtgggat gaataccgac gtctgatggc cgtagaacaa taaccaggct
    23521 tttgtaaaga cgaacaataa atttttacct tttgcagaaa ctttagttcg gaacttcggg
    23581 ctataaaacg aatctgaaga acacagcaat tttgcgttat ctgttaatcg agactgaaat
    23641 acatgaaaaa aaccacatta gcactgagtg cactggctct gagtttaggt ttggcgttat
    23701 ctccgctctc tgcaacggcg gctgagactt cttcagcaac gacagcccag cagatgccaa
    23761 gccttgcacc gatgctcgaa aaggtgatgc cttcagtggt cagcattaac gtagaaggta
    23821 gcacaaccgt taatacgccg cgtatgccgc gtaatttcca gcagttcttc ggtgatgatt
    23881 ctccgttctg ccaggaaggt tctccgttcc agagctctcc gttctgccag gggggccagg
    23941 gcggtaatgg cggcggccag caacagaaat tcatggcgct gggttccggc gtcatcattg
    24001 atgccgataa aggctatgtc gtcaccaaca accacgttgt tgataacgcg acggtgatta
    24061 aagtccagct gagcgatggt cgtaagttcg acgcgaagat ggttggcaaa gatccgcgct
    24121 ctgatatcgc gctgatccaa atccagaacc cgaaaaaact gaccgcaatt aagatggcgg
    24181 attctgatgc actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg tttggtctgg
    24241 gcgagacggt aacttccggg attgtctctg cgctggggcg tagcggcctg aatgctgaaa
    24301 actacgaaaa cttcatccag accgatgcag cgatcaaccg tggtaactcc ggtggtgcgc
    24361 tggttaacct gaacggcgaa ctgatcggta tcaacaccgc gatcctcgca ccggacggcg
    24421 gcaacatcgg tatcggtttt gctatcccga gtaacatggt gaaaaacctg acctcgcaga
    24481 tggtggaata cgggcaggtg aaacgcggtg agctgggtat tatggggact gagctgaatt
    24541 ccgaactggc gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta agccaggttc
    24601 tgcctaattc ctcggctgca aaagcgggca ttaaagcggg tgatgtgatc acctcactga
    24661 acggtaagcc gatcagcagc tttgccgcac tgcgtgctca ggtgggtact atgccggtgg
    24721 gcagcaaact gaccctgggc ttactgcgcg acggtaagca ggttaacgtg aacctggaac
    24781 tgcagcagag cagccagaat caggttgatt ccagctccat cttcaacggc attgaaggcg
    24841 ctgagatgag caacaaaggt aaagatcagg gcgtggtagt gaacaacgtg aagacgggca
    24901 ctccggctgc gcagatcggc ctgaagaaag gtgatgtgat tattggtgcg aaccagcagg
    24961 cagtgaaaaa catcgctgaa ctgcgtaaag ttctcgacag caaaccgtct gtgctggcac
    25021 tgaacattca gcgcggcgac agcaccatct acctgttaat gcagtaatct ccctcaaccc
    25081 cttcctgaat acgggaaggg gttctcctta caatctgtga acttcgccac aactccatac
    25141 atcttcatca tcctttgggc atttgcacaa tgccgtacgt tacgtacttc cttatgctaa
    25201 gccgtgcata acggaggact tatggctggc tggcatcttg ataccaaaat ggcgcaggat
    25261 atcgtggcac gtaccatgcg catcatcgat accaatatca acgtaatgga tgcccgcggg
    25321 cgaattatcg gcagcggcga tcgtgagcgt attggtgaat tgcacgaagg tgcattgctg
    25381 gtactttcac agggacgagt cgtcgatatc gatgacgcgg tagcacgtca tctgcacggt
    25441 gtgcggcagg ggattaatct accgttacgg ctggaaggtg aaattgtcgg cgtaattggc
    25501 ctgacaggtg aaccagagaa tctgcgtaaa tatggcgaac tggtctgcat gacggctgaa
    25561 atgatgctgg aacagtcgcg gttgatgcac ttgttggcgc aggatagccg tttgcgggaa
    25621 gaactggtga tgaacctgat tcaggcagag gagaatactc ccgcacttct gaatgggcgc
    25681 aacggctggg gatcgatctc aatcaaccgc gagtggtggc tattgttgag gtcgacagcg
    25741 gtcagcttgg cgtggacagc gcaatggcgg agttacaaca actgcaaaac gcgctgacta
    25801 cgcccgagcg taataatctg gtggcgattg tctcgctaac cgaaatggtg gtgttgaaac
    25861 cggcgttgaa ctcttttggg cgctgggatg cagaagatca tcgtaagcga gttgaacaac
    25921 tgattacccg catgaaagag tacggccagc tgcgttttcg cgtttcactg ggcaactatt
    25981 ttaccggtcc tagcagtatt gcccgatcct atcgtacggc gaaaacgacg atggtggtgg
    26041 gtaaacagcg gatgccagaa agtcgctgct atttttatca ggatctgatg ttacctgtgt
    26101 tactcgacag tttgcgtggc gactggcagg ccaacgaact ggcgcgaccg ctggcgcggc
    26161 tgaaagcgat ggacaataac ggcttgctgc gacgaacgct ggcggcgtgg tttcgccaca
    26221 atgtgcaacc gctggcaacg tcaaaggcgt tgtttattca tcgtaatacc ctggagtatc
    26281 ggcttaatcg tatatcggaa ctgaccgggc ttgatttggg caattttgat gacaggttgc
    26341 tgctgtatgt ggcgttacaa ctggatgaag agcggtagat tatgcgttaa ggttgaggcg
    26401 gcgctggctc attcccctca gcccgaaggg gagaggggaa tgcccgagtt aaccgtcaat
    26461 tcgggattat ttacgcgtta gtttttccag atcggcttca atctcgctga tcttattggt
    26521 tacgactgac tccaggtgac gtaagtcgtc gaggatctta cgcttaagat caacttcgct
    26581 gcggtcacgc tgacagattt gatcaagctc atcaatgata taccgtagat tcgggctgat
    26641 ttcctggact tctttataac cctgacccac accatcagcg acgaccgttt tacgctgacg
    26701 cggatattta aacttaacgc tcttggcgaa aaactcgcct ttgtctttct ggaaatagat
    26761 tttcagaata tcgttgttgg cttcctgccg gaggctgtaa cgatcaattt cttcaggatt
    26821 ggtaataccc agacttttca gattgtcgta catagcgtta cctcaaaatg agtcagtaaa
    26881 ttgtgcttat tttagcattt ggcttggccc gcccggctcg actgtgatcg cagaaagcct
    26941 ttttcttcca gacttctatc atggcgtaaa aatcaaaaaa ttacctgctt tattgtggcg
    27001 atattccatg ggatggatta gcacatttat cttcgttttg cgaattacat gacagtccga
    27061 aacaagatga aaaagtattt aaaaaagggg gaggaagaat gagtaggacg aattattctg
    27121 agccagaaag catacgggca gcatcatgct gcccgtatat gtactcttaa tcgatggtac
    27181 gcagcagttc gttaatgccg actttgccgc gagttttcgc gtcaactttc ttaacgataa
    27241 ccgcacagta gaggctgtat ttgccatctt ttgacggcag attacctgaa acaaccacag
    27301 accccgccgg aacgcgaccg tagtggattt cgccggtttc acggtcgtaa atacgggtgc
    27361 tctgaccaat gtatacgccc atagagatga cggaaccttc ttcgacaatc accccttcaa
    27421 ccacttcaga gcgtgcgcca atgaagcagt tatcttcaat aatggtcggg ttagcctgca
    27481 gcggctccag cacgccgccg atgcccacgc caccggaaag gtggacgttt ttaccaatct
    27541 gcgcacaaga accgacggtc gcccaggtat caaccatggt gccttcatca acatatgcgc
    27601 cgatgttaac gtaagacggc atcagcacgg tgttacgggc aataaacgca ccctgacgta
    27661 ccgccgctgg tggcacaacg cggaagcctt ctttctggaa acgtgcttcg tcgtagtcgg
    27721 cgaatttcat cggcactttg tcgaagtagc ggctttctgc cccttcgatc acctgattat
    27781 cattaatacg gaaagagagc agcaccgctt ttttcaacca ctgatgcgtc acccactgac
    27841 cgtcaatttt ttccgctaca cgcagtgcgc cggaatccag cagggcgatc acctgattta
    27901 ccgcttcgcg ggtaacggtg tctgcattgg ctggcgtgat ctcggcacgg cgttcaaaag
    27961 cggtttcaat aatgttctgt aactgctgca ttgttaaact cttttcatat cagtaaacac
    28021 atcacccttt atcgtttgga ttgagggcct ctgtcaaccg ctgatgcact tcctgctgca
    28081 actcgttatt aagcgcacgc cggtcagcgg tggcaattat gaataaatct tctactcgct
    28141 cgccaatggt tgtaattcgg gcaccatgaa gcgaaattcc cagatcggca aaaattttcc
    28201 cgactcgcgc cagcagtcca ggttggtcga gggcgatcag ttcgaggaac gatttgcggt
    28261 cggtatgggt cggcaaaaac gttacttcgg tttcaacagt aaaatggcgt aatttggcgg
    28321 gttggcgacg gggctgcggt ggctgccagc tactttgcgt cagcacctgc tccagaccaa
    28381 accgaataac ctcatggcga tctgcggaca gcgggctgcc atcgggttcc agcacgataa
    28441 aggtatccat cgccataccg tcgcgagtgg tgaaaatttg tgcgtcgtga acacttaaat
    28501 tgcggcggtc taattcggca cagacggcgg caaacagata ggggcggtcc gggctccaga
    28561 taaaaatctc ggtgcctcca cgcgtagcct gcgggctaag caataccagc ggtttgctta
    28621 aatcatgctg taataaatgg cgggcatgcc aggccagttg atttgggcta tggcggacaa
    28681 aatagttagc acgacagcgt gaccaaattt ggtgcagcgc ctcttcgtcg atgttatcca
    28741 tgcgcagtag tgccagtgcc tggagttggt gatggcgaac ccgttcgcgc atatccggcg
    28801 tgttttgcat tccgcgtcgt agctgctttt cggtggcaaa atagagttcg cgcaacagac
    28861 tttgcttcca gctattccac agcgtttcgt tggtggcgca aatgtcagcc actgtcaggc
    28921 ataccagata gcgcagacga ttttccgttt gaacttcttc ggcaaactgc ttgatgactt
    28981 cagggtcctg aatatcgcgg cgttgtgctg tcaccgacat cagcaagtgc tggcgaacca
    29041 gccaggcaac cagctgtgtt tcgcgtgagt tcagcccgtg gagttctgca aaatgcacta
    29101 catcctgagc accgagaatg gagtggtcgc cgccgcgtcc tttggcgata tcgtgaaaca
    29161 gcgcggcgat gaaaatcagc tcagttgacg gcaggcgcgg ccagacgtcc acacacaacg
    29221 gatggcgctg gcgcgtttct tcactggcaa aactctccag tttcagcatc acgcggatag
    29281 tatgttcatc caccgtgtag gcgtggaaca gatcaaactg catctgcccg acgatatgcg
    29341 accattgcgg catatacgcg ccgagcacgc tatggcgatg cattggcaat agcccgcgcc
    29401 gcaccgctcc ggggtgacgc agaatgctca aaaacagttt tcgtgcttgc ggaatattac
    29461 acagcggttg ttgcagatgg cgacgggcat ggcgtaactg gcgcagcgtg gtggagtaaa
    29521 taccggtgat cgcactgttg cgcaccatgg tgtagaacat acgcaagatg gcttccggct
    29581 ggcgcataaa tagtgtttca tcacgcaggt cgattagcgt accgcgtagc tgaaactcat
    29641 cgtcgagtgg acgtggtttt tcgtcggcgg gaagggcgag gatggcttca tcgaacagtt
    29701 gcagcagcat ctggttgagt tcactgacgc ggcgtgtaac gcggaagtaa tccttcatca
    29761 tccgctcgac cggttcgttg ccttcaccac tgtaattcag acgctgagcg acgctaagtt
    29821 ggcgatcgaa taacagacga ttatcgtaac ggctgacgac cagatgcagg gcaaagcgaa
    29881 tacgccacag tatatgcaga cattcgttta attccgcccg ctccgctgag gttaagaagc
    29941 caaacccgac catttcatcc agcgatgttg cgccaaaatg acggcgggcc acccattgca
    30001 gagtgtgaat atcgcgcaag ccgccagggc tacttttgat gtctggttca aggttgtagc
    30061 tggtgccatg gtaacgctga tggcgctggt tctgttcttc aactttcgct gcgtagaact
    30121 tgtcggaagg ccagaatcct tcgctaaaaa tatgtttttg cagttcgagg aagagcgcaa
    30181 catcgccaat taataagcgg gattcgatta aattggtggc gacggttaaa tccgataacc
    30241 cttccaacat gcactcttca agcgtgcgca cactatgacc gacttccagc tttacatccc
    30301 agagcagcgt taacagctcg cccacttttt gcgcctgatc gtccgggagc tttttacggc
    30361 ttaaaatcag taaatcgatg tcagaaagtg gatgcagctc gccacgaccg tagccaccga
    30421 cggcaaccaa tgccaggtcg gcaatctggc tgaatcccgc ttcaatccat aatcgttgca
    30481 ggagctggtc tatgaactcg gtacgtgcct caatcaactg ttctgcagag atcccattgt
    30541 caaaggcatc acccagccaa cgctggaaag tatcgatatg ggcttttatc ccacagacgg
    30601 ttaattcatc acggggccag gcgcatggat tttgcggttg accgggcagg gtggagagag
    30661 cggtgtttgc gtactgttct ggaagggtat tcattgtgcg ccacccataa gattaaatta
    30721 tcgcattaaa aaagccggca ttcgccggct tcatcttatt cgtcgtgcga gattatcgcc
    30781 gggatggtgt catccttgcg tagcgtcaaa atttcgcagc cgttatcagt caccacaata
    30841 gtatgctcat attgtgcaga caagctgcga tctttggttt ttaccgtcca gccatctttc
    30901 atggtgcgga tctctttttt acccgcgttg accattggct caatggtgaa cgtcatcccc
    30961 ggtttcagca cgacgttggt ttcacgggag tcatagtgca gcacctgcgg ttcttcatgg
    31021 aagccgcgac caataccgtg tccgcaatat tcacgaacga cggagaagcc ttctgcttcg
    31081 acaaatttct gaatcgccgc accgatttcg cgcagattaa tgcctggttt taccatgcgt
    31141 agcgccaggt acaggctttc ttgcgtgatg cggcacaggc gttcgcccat gatggtcggc
    31201 ttaccgacga taaacatttt cgaggtatcg ccgtggaaac catctttgat tacggtgaca
    31261 tcaatgttaa cgatatcgcc atctttcagc agcttagcat cgtccgggat accgtggcac
    31321 accacttcat taatagagat gcaaacggat ttcggatagc cgtgatagcc gaggcaggca
    31381 gaaaccgcgt gttgttcatt aacaatgtaa tcattacaga tgcgatccag ctcgccggtg
    31441 ctgacgcccg gtttaacata cggttcgatc atctccagca cttcggcagc cagtcggcca
    31501 gcgacgcgca ttttttcgat atcttctggg gtcttgattg agatagccat taattctgtc
    31561 catcagcgtc ggtgataccg acaatatata tgtaagtgcc gtcaatggta tcacacccgg
    31621 gcaaattgag aatcattctg aatttcgcca aacgtgccac tgaaggtttt ctataataga
    31681 aaattcgacg tctgatgctg tacacagcgc caacaattat tggtgtccac gacgtatttg
    31741 tggtataaag cgcgccggac ttccgatcca tttcgtatac acagactgga cggaagcgac
    31801 aatctcactt tgtgtaacaa cacacacgta tcggcacata ttccggggtg ccctttgggg
    31861 tcggtaatat gggatacgtg gaggcataac cccaactttt atatagaggt tttaatcatg
    31921 gcaactgttt ccatgcgcga catgctcaag gctggtgttc acttcggtca ccagacccgt
    31981 tactggaacc cgaaaatgaa gccgttcatc ttcggtgcgc gtaacaaagt tcacatcatc
    32041 aaccttgaga aaactgtacc gatgttcaac gaagctctgg ctgaactgaa caagattgtt
    32101 tctcgcaaag gtaaaatcct tttcgttggt actaaacgcg ctgcaagcga agcggtgaaa
    32161 gacgctgctc tgagctgcga ccagttcttc gtgaaccatc gctggctggg cggtatgctg
    32221 actaactgga aaaccgttcg tcagtccatc aaacgtctga aagacctgga aactcagtct
    32281 caggacggta ctttcgacaa gctgaccaag aaagaagcgc tgatgcgcac tcgtgagctg
    32341 gagaaactgg aaaacagcct gggcggtatc aaagacatgg gcggtctgcc ggacgctctg
    32401 tttgtaatcg atgctgacca cgaacacatt gctatcaaag aagcaaacaa cttgggtatt
    32461 ccggtatttg ctatcgttga taccaactct gatccggacg gtgttgactt cgttatcccg
    32521 ggtaacgacg acgcaatccg tgctgtgacc ctgtacctgg gcgctgttgc tgcaaccgta
    32581 cgtgaaggcc gttctcagga tctggcttcc caggcggaag aaagcttcgt agaagctgag
    32641 taataaggct tgataactcc cccaaaatag ttcgagttgc agaaaggcgg caagctcgag
    32701 aattcccggg agcttacatc agtaagtgac cgggatgagc gagcgaagac aacgcatctg
    32761 cggcgcgaaa tatgaagggg gagagccctt atagaccagg tagtacacgt ttggttaggg
    32821 ggcctgcata tggccccctt tttcactttt atatctgtgc ggtttaatgc cgggcagatc
    32881 acatctccga ggattttaga atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg
    32941 agcgtactgg cgcaggcatg atggattgca aaaaagcact gactgaagct aacggcgaca
    33001 tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg aaaaaagcag
    33061 gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac ggcatcattc
    33121 tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag
    33181 acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac
    33241 agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc
    33301 gcgttgctgc gctggaaggc gacgttctgg gttcttatca gcacggtgcg cgtatcggcg
    33361 ttctggttgc tgctaaaggc gctgacgaag agctggttaa acacatcgct atgcacgttg
    33421 ctgcaagcaa gccagaattc atcaaaccag aagacgtatc cgctgaagtg gtagaaaaag
    33481 aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa atcgcagaga
    33541 aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt
    33601 tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga
    33661 ctggcttcat ccgtttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag
    33721 cagaagttgc tgcgatgtcc aagcagtctt aattatcaaa aaggagccgc ctgagggcgg
    33781 cttctttttg tgcccatctt gtaaattcag ctaacccttg tggggctgcg ctgaaaagcg
    33841 acgtacaatg tcgctagtat taattcattt caatcgttga cagtctcagg aaagaaacat
    33901 ggctaccaat gcaaaacccg tctataaacg cattctgctt aagttgagtg gcgaagctct
    33961 gcagggcact gaaggcttcg gtattgatgc aagcatactg gatcgtatgg ctcaggaaat
    34021 caaagaactg gttgaactgg gtattcaggt tggtgtggtg attggtgggg gtaacctgtt
    34081 ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc acatggggat
    34141 gctggcgacc gtaatgaacg gcttggcaat gcgtgatgca ctgcaccgcg cctatgtgaa
    34201 cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc gacagctaca gctgggcaga
    34261 agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta caggtaaccc
    34321 gttctttacc accgactcag cagcttgcct gcgtggtatc gaaattgaag ccgatgtggt
    34381 gctgaaagca accaaagttg acggcgtgtt taccgctgat ccggcgaaag atccaaccgc
    34441 aaccatgtac gagcaattga cttacagcga agtgctggaa aaagagctga aagtcatgga
    34501 cctggcggcc ttcacgctgg ctcgtgacca taaattaccg attcgtgttt tcaatatgaa
    34561 caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt taatcacgga
    34621 ataattcccg tgatggataa ataagggtaa gattccgcgt aagtatcgcg ggggcgtaag
    34681 tctggttata aggcgttatt gttgcaggca gtttggtcac ggccagcgcg cagcaaccgg
    34741 agcgtacaaa agtacgtgag gatggcgagc actgctcggg gccaaaatgg caaataaaat
    34801 agcctaataa tccagacgat tacccgtaat atgtttaatc agggctatac ttagcacact
    34861 tccactgtgt gtgactgtct ggtctgactg agacaagttt tcaaggattc gtaacgtgat
    34921 tagcgatatc agaaaagatg ctgaagtacg catggacaaa tgcgtagaag cgttcaaaac
    34981 ccaaatcagc aaaatacgca cgggtcgtgc ttctcccagc ctgctggatg gcattgtcgt
    35041 ggaatattac ggcacgccga cgccgctgcg tcagctggca agcgtaacgg tagaagattc
    35101 ccgtactctg aaaatcaacg tgtttgatcg ttcaatgtct ccggccgttg aaaaagcgat
    35161 tatggcctcc gatctcggcc tgaacccgaa ctctgcgggt agcgacatcc gtgttccgct
    35221 gccgccgctg acggaagaac gtcgtaaaga tctgaccaaa atcgttcgtg gtgaagcaga
    35281 acaagcgcgt gttgcagtac gtaatgtgcg tcgtgacgcg aacgacaaag tgaaagcact
    35341 gttgaaagat aaagagatca gcgaagacga cgatcgccgt tctcaggacg atgtacagaa
    35401 actgactgat gctgcaatca agaaaattga agcggcgctg gcagacaaag aagcagaact
    35461 gatgcagttc tgatttcttg aacgacaaaa acgccgctca gtagatcctt gcggatcggc
    35521 tggcggcgtt ttgcttttta ttctgtctca actctggatg tttcatgaag caactcacca
    35581 ttctgggctc gaccggctcg attggttgca gcacgctgga cgtggtgcgc cataatcccg
    35641 aacacttccg cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg gtagaacagt
    35701 gcctggaatt ctctccccgc tatgccgtaa tggacgatga agcgagtgcg aaacttctta
    35761 aaacgatgct acagcaacag gggagccgca ccgaagtctt aagtgggcaa caagccgctt
    35821 gcgatatggc agcgcttgag gaggttgatc aggtgatggc agccattgtt ggtgctgctg
    35881 ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac cattttgctg gccaataaag
    35941 aatcactggt tacctgcgga cgtctgttta tggacgccgt aaagcagagc aaagcgcaat
    36001 tgttaccggt cgatagcgaa cataacgcta tttttcagag tttaccgcaa cctatccagc
    36061 ataatctggg atacgctgac cttgagcaaa atggcgtggt gtccatttta cttaccgggt
    36121 ctggtggccc tttccgtgag acgccattgc gcgatttggc aacaatgacg cccgatcaag
    36181 cttgccgtca tccgaactgg tcgatgggac gtaaaatttc tgtcgattcg gctaccatga
    36241 tgaacaaagg tctggaatac attgaagcgc gttggctgtt taacgccagc gccagccaga
    36301 tggaagtgct gattcacccg cagtcagtga ttcactcaat ggtgcgctat caggacggca
    36361 gtgttctggc gcagctgggg gaaccggata tgcgtacgcc aattgcccac accatggcat
    36421 ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta agtgcgttga
    36481 catttgccgc accggattat gagcgttatc catgcctgaa actggcgatg gaggcgttcg
    36541 aacaaggcca ggcagcgacg acagcattga atgccgcaaa cgaaatcacc gttgctgctt
    36601 ttcttgcgca acaaatccgc tttacggata tcgctgcgtt gaatttatct gtactggaaa
    36661 aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat gcgaacgcga
    36721 gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg aggataatcc ggctacagag
    36781 agtcgcgcta tttgttagcg tagggcttca atgatatagt ctgcgccatc tgatcgtaag
    36841 tagttggctt tataaggtca ggtatgccgt ggttttacac ggcttttttt tgtataggct
    36901 tcagtattcc tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga tgttgtctgc
    36961 tactcaacca cttagcgaaa aattgccagc gcatggctgc cgtcatgttg cgatcattat
    37021 ggacggcaat ggtcgctggg caaaaaagca agggaagatt cgtgcctttg ggcataaagc
    37081 cggggcaaaa tccgtccgcc gggctgtctc ttttgcggcc aacaacggta ttgaagcgtt
    37141 aacgctgtat gcctttagta gtgaaaactg gaaccgacca gcgcaggaag tcagtgcgtt
    37201 aatggaactg tttgtgtggg cgctcgatag cgaagtaaaa agtctgcacc gacataacgt
    37261 gcgtctgcgt attattggcg ataccagtcg ttttaactcg cgtttgcaag aacgtattcg
    37321 taaatctgaa gcgctaacag ccggaaatac cggtctgacg ctgaatattg cggcgaacta
    37381 cggtggacgt tgggatatag tccagggagt caggcaactg gctgaaaagg tgcagcaagg
    37441 aaacctgcaa ccagatcaga tagatgaaga gatgttaaac cagcatgtct gtatgcatga
    37501 actggcccct gtagatttag taattaggac tgggggagag catcgcatta gtaacttttt
    37561 gctttggcaa attgcctatg ccgaacttta ctttacagat gttctctggc ccgatttcga
    37621 tgaacaagac tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc gtttcggcgg
    37681 caccgagccc ggtgatgaaa cagcctgatg ggggtcgctt ttgctgaagt atcgcctgat
    37741 atctgctttt gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc cgccggtggg
    37801 gttcgccatt gtaacgctgg tggtctgcat gctggcagcg tgggaatggg gacagcttag
    37861 cggttttacc actcgttcgc agcgagtatg gttggcggtg ttatgcgggt tattgttggc
    37921 gctgatgctt tttctgttgc cggaatatca ccgaaatatt catcaaccgc tggttgaaat
    37981 ctcactttgg gcttcgttgg gttggtggat tgtcgcgcta ttgctggtgc tgttttaccc
    38041 aggttccgca gcaatctggc gtaactctaa aacattgcgc cttatttttg gcgtgctaac
    38101 cattgttccc ttcttctggg gcatgctggc gttacgggcc tggcactatg acgagaatca
    38161 ttacagtggc gcaatatggc tgctctatgt catgatcctg gtatggtgcg ctgactccgg
    38221 cgcatatatg tttggcaaat tgtttggtaa acataagctg gcaccgaagg tttctccggg
    38281 taaaacctgg caaggcttta taggtggact cgctactgca gcggtaatct catggggtta
    38341 tggcatgtgg gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct ctattgtcgc
    38401 agcgttagcc tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg aagcaggaat
    38461 taaggacagc ggtcatttaa ttccaggaca cggtggtatt ttagatcgta ttgatagcct
    38521 gacggctgcg gtaccggtct ttgcttgctt gttgttactg gtattcagga cgctttaacg
    38581 gaaggtaata tgctgagttt tctctgggat ttggcttcgt tcatcgttgc actgggtgta
    38641 cttatcaccg tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg tgttcgcgtt
    38701 gagcgtttct caatagggtt tggtaaggcg ctctggcggc gaactgataa gctcggcacc
    38761 gaatatgtta tcgccctgat cccgttgggc ggttatgtca aaatgctgga tgagcgcgca
    38821 gaaccggtcg ttccggaact ccgccaccat gccttcaata ataaatctgt cggccaacga
    38881 gtggcgatta ttgccgcagg tccggttgca aacttcattt ttgctatctt tgcctactgg
    38941 ctggttttta ttattggtgt gcctggcgta cgtccggtgg ttggcgaaat agcagccaat
    39001 tcgatagctg cggaagcaca aattgcacca ggtacggaac taaaagccgt agatggtatc
    39061 gaaacgcctg attgggatgc cgtgcgtttg cagttggtcg ataaaattgg cgatgaaagc
    39121 accaccatta cagtagcgcc atttggcagc gaccaacggc gggatgtaaa gctcgattta
    39181 cgtcactggg cgtttgagcc tgataaagaa gatccggtat cttcgctggg gattcgtcct
    39241 cgtgggccgc aaattgaacc tgtactggaa aatgtgcagc caaactcggc ggcaagcaag
    39301 gcaggtttgc aagcaggcga caggatcgtt aaagtcgatg gtcagccctt aacgcagtgg
    39361 gtgacctttg tgatgcttgt ccgggataac ccgggtaaat ccttagcgtt agaaatcgaa
    39421 aggcagggga gtcccttgtc tttgacatta atcccggaga gtaaaccggg taatggtaaa
    39481 gcgattggtt ttgtcggtat tgagccgaaa gtcattcctt tgccagatga gtataaagtt
    39541 gtacgccagt atgggccgtt caacgccatc gtcgaagcca cggacaaaac gtggcagctg
    39601 atgaagctga cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa actgaacaac
    39661 ctcagtgggc cgatttctat cgccaagggg gctgggatga cagcggaact cggggtagtt
    39721 tattacctgc cgtttcttgc gcttattagc gtgaacttag ggataattaa cctgtttccg
    39781 ttgcccgtac ttgacggggg gcatctgctg ttccttgcga tcgaaaagat caagggcgga
    39841 ccggtatccg agcgggttca agacttttgt tatcgcattg gctcgattct gctggtgctg
    39901 ttaatggggc ttgcactttt caatgatttc tctcggttat gagagttagt taggaagaac
    39961 gcataataac gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt agcagcgcca
    40021 ccgtatacgg tgctgaaggg ttcgtagtga aagatattca tttcgaaggc cttcagcgtg
    40081 tcgccgttgg tgcggccctc ctcagtatgc cggtgcgtac aggcgacacg gttaatgatg
    40141 aagatatcag taataccatt cgcgctctgt ttgctaccgg caactttgag gatgttcgcg
    40201 tccttcgtga tggtgatacc cttctggttc aggtaaaaga acgtccgacc attgccagca
    40261 ttactttctc cggtaacaaa tcggtgaaag atgacatgct aaagcaaaac ctcgaggctt
    40321 ctggtgtgcg tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc gagaaaggtc
    40381 tggaagactt ctactacagc gtcggtaaat atagcgccag cgttaaagct gtcgtgaccc
    40441 cgctgccgcg caaccgtgtt gacctaaaac tggtgttccg ggaaggtgtg tcagctgaaa
    40501 tccagcaaat taacattgtt ggtaaccatg ctttcaccac cgacgaactg atctctcatt
    40561 tccaactgcg tgacgaagtg ccgtggtgga atgtggtagg cgatcgtaaa taccagaaac
    40621 agaaactggc gggcgacctt gaaaccctgc gcagctacta tctggaccgc ggttatgccc
    40681 gtttcaacat cgactctacc caggtcagtc tgacgccaga taaaaaaggt atttacgtca
    40741 cggtgaacat caccgaaggc gatcagtaca agctttctgg cgttgaagtg agcggcaacc
    40801 ttgccgggca ctccgctgaa attgagcagc tgactaagat cgagccgggc gagctgtata
    40861 acggcactaa agtgaccaaa atggaagatg acattaaaaa gcttctcggt cgctatggtt
    40921 atgcctatcc gcgcgtacag tcgatgcctg aaattaacga tgccgacaaa accgttaaat
    40981 tacgcgtgaa cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt tttgaaggta
    41041 acgatacctc gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa ggtgcatggt
    41101 tggggagcga tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc ttctttgaaa
    41161 ctgtcgatac tgatacccaa cgtgttccgg gtagcccgga ccaggttgat gtagtctaca
    41221 aggtgaaaga acgtaacact ggtagcttca acttcgggat tggttacggt actgaaagtg
    41281 gcgtgagctt ccaggctggc gtgcaacagg ataactggtt aggtacaggt tatgctgttg
    41341 gtatcaacgg gaccaaaaac gattaccaga cctatgctga actgtcggta accaacccgt
    41401 acttcaccgt agatggcgta agcctcggtg gtcgtctctt ctataatgac ttccaggcag
    41461 atgacgccga cctgtccgac tataccaaca agagttatgg tacagacgtg acgttgggct
    41521 tcccgattaa cgaatataac tcgctgcgtg caggtctggg ttatgtacat aactccctgt
    41581 ccaacatgca gcctcaggtt gcgatgtggc gttatctgta ctctatgggt gaacatccga
    41641 gcacttctga tcaggataac agcttcaaaa cggacgactt cacgttcaac tatggttgga
    41701 cctataacaa gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc aacctgaccg
    41761 gtaaagtgac cattcctgga tcggataacg aatactacaa agtgacgtta gacacggcga
    41821 cttatgtgcc gatcgatgac gatcacaaat gggttgttct gggtcgtacc cgctggggtt
    41881 atggtgatgg tttaggcggc aaagagatgc cgttctacga gaacttctat gccggtggtt
    41941 ccagcaccgt gcgtggcttc cagtccaata ccattggtcc gaaagcagtt tacttcccgc
    42001 atcaggccag taattatgat ccggactatg attacgaatg tgcgactcag gacggcgcga
    42061 aagacctgtg taaatcggat gatgctgtag gcggtaacgc catggcggtt gccagcctcg
    42121 agttcatcac cccgacgccg tttattagcg ataagtatgc taactcggtt cgtacttcat
    42181 tcttctggga tatgggtacc gtttgggata caaactggga ttccagccaa tattctggat
    42241 atccggacta tagtgatcca agcaatatcc gtatgtctgc gggtatcgca ttacaatgga
    42301 tgtccccatt ggggccgttg gtgttctcct acgcccagcc gttcaaaaag tacgatggag
    42361 acaaggcaga acagttccag tttaacatcg gtaaaacctg gtaagtgttc tccacaaagg
    42421 aatgtagtgg tagtgtagcg atgactttag gcgatcaata taagatcgcc gggccacgca
    42481 aagaactgca ccctccggtg caaatgggat ggtaaggagt ttattgtgaa aaagtggtta
    42541 ttagctgcag gtcttggttt agcactggca acttctgctc aggcggctga caaaattgca
    42601 atcgtcaata tgggcagcct gttccagcag gtagcgcaga aaaccggtgt ttctaacacg
    42661 ctggaaaatg agttcaaagg ccgtgccagc gaactgcagc gtatggaaac cgatctgcag
    42721 gctaaaatga aaaaactgca gtccatgaaa gcgggcagcg atcgcaccaa actggaaaaa
    42781 gacgtgatgg ctcagcgcca gacttttgct cagaaagcgc aggcttttga gcaggatcgc
    42841 gcacgtcgtt ccaacgaaga acgcggcaaa ctggttactc gtatccagac tgctgtgaaa
    42901 tccgttgcca acagccagga tatcgatctg gttgttgatg caaacgctgt tgcttacaac
    42961 agcagcgatg taaaagacat cactgccgac gtactgaaac aggttaaata agtaatgcct
    43021 tcaattcgac tggctgattt agcgcagcag ttggatgcag aactacacgg tgatggcgat
    43081 atcgtcatca ccggcgttgc gtccatgcaa tctgcacaaa caggtcacat tacgttcatg
    43141 gttaacccaa aataccgtga gcatttaggc ttgtgccaag cgtccgcggt tgtcatgacc
    43201 caggacgatc ttcctttcgc gaaaagtgcc gcgctggtag tgaagaatcc ctacctgact
    43261 tacgcgcgca tggcgcaaat tttagatacc acgccgcagc ccgcgcagaa cattgcaccc
    43321 agtgcggtga tcgacgcgac ggcgaagctg ggtaacaacg tatcgattgg cgctaacgcg
    43381 gtgattgagt ccggcgttga actgggcgat aacgtgatta tcggtgccgg ttgcttcgta
    43441 ggtaaaaaca gcaaaatcgg tgcaggttcg cgtctctggg cgaacgtaac catttaccat
    43501 gagatccaga tcggtcagaa ttgcctgatc cagtccggaa cagtggtagg cgcagacggc
    43561 tttggttatg ccaacgatcg tggtaactgg gtgaagatcc cacagattgg tcgcgtaatt
    43621 attggcgatc gagtggagat cggtgcctgc acaaccatcg atcgcggcgc gctggatgac
    43681 actgttattg gcaatggcgt gatcattgat aaccagtgcc agattgcaca taacgtcgtg
    43741 attggcgaca atacggcggt tgccggtggc gtcattatgg cgggcagcct gaaaattggt
    43801 cgttactgca tgatcggcgg agccagcgta atcaacgggc atatggaaat atgcgacaaa
    43861 gtgacggtta cgggcatggg tatggtgatg cgtcccatca ctgaaccagg cgtctattcc
    43921 tcaggcattc cgctgcaacc caacaaagtc tggcgcaaaa ccgctgcact ggtgatgaac
    43981 attgatgaca tgagcaagcg tctgaaatcg cttgagcgca aggttaatca acaagactaa
    44041 cgttccatct tttgttcgcc aaactttacg gcctgtctca ttcttacgat tgcggcaggc
    44101 cgtgttatta ttgtcgtttc ttatattttg acaggaagag tatcttgact actaacactc
    44161 atactctgca gattgaagag attttagaac ttctgccgca ccgtttcccg ttcttactgg
    44221 tggatcgcgt gctggatttt gaagaaggtc gttttctgcg cgcagtaaaa aatgtctctg
    44281 tcaatgagcc attcttccag ggccatttcc ctggaaaacc gattttcccg ggtgtgctga
    44341 ttctggaagc aatggcacag gcaacaggta ttctggcgtt taaaagcgta ggaaaactgg
    44401 aaccgggtga gctgtactac ttcgctggta ttgacgaagc gcgcttcaag cgcccggtcg
    44461 tgcctggcga tcaaatgatc atggaagtca ctttcgaaaa aacgcgccgc ggcctgaccc
    44521 gttttaaagg ggttgctctg gtcgatggta aagtagtttg cgaagcaacg atgatgtgtg
    44581 ctcgtagccg ggaggcctga tacgtgattg ataaatccgc ctttgtgcat ccaaccgcca
    44641 ttgtggaaga gggcgcgtca attggcgcga acgcacacat tggtcctttt tgtatcgttg
    44701 gaccccatgt cgaaattggt gagggtaccg tactgaaatc tcacgttgtc gtgaatggtc
    44761 atactaaaat tggccgcgat aatgagattt atcagtttgc ctccatcggc gaagttaacc
    44821 aggatttgaa atatgctggc gaaccgaccc gtgtggaaat cggcgatcgt aaccgcattc
    44881 gcgaaagcgt caccattcat cgtggcacag tccagggcgg tggattgacg aaggtgggca
    44941 gcgacaactt actgatgatc aacgcgcaca ttgcgcacga ttgtacggta ggtaaccgct
    45001 gtattctcgc caacaacgca acgctggcgg gtcacgtatc ggttgacgac ttcgcgatca
    45061 tcggcggcat gaccgcagtc catcagttct gcatcattgg tgcgcatgtg atggttggcg
    45121 gctgctccgg tgtggcgcag gacgtccctc cttatgtcat tgcgcagggt aaccacgcaa
    45181 cgccgttcgg tgtcaatatc gaagggctga agcgccgcgg attcagccgt gaggcgatta
    45241 ccgctatccg caatgcgtat aagctaattt atcgtagcgg taaaacgctc gatgaagtga
    45301 aaccggaaat tgctgaactg gcggaaacat atccggaagt gaaagccttt accgatttct
    45361 ttgcacgctc aacgcgcggt ctgattcgtt aatgactgac cagcgtccat taacgattgc
    45421 cctggtcgcc ggagaaatct ccggcgatat cctgggggcc ggtttaatcc gcgctctgaa
    45481 agaacgtgtg cccaacgccc gctttgttgg tgttgccggg ccacgaatgc aggctgaagg
    45541 ctgcgaagcc tggtacgaaa tggaagaact ggcggtgatg ggcattgttg aagtgctcgg
    45601 tcgtctgcgt cgcttactgc atattcgtgc cgatctgaca aagcgttttg gcgaactgaa
    45661 gccagatgtt tttgttggta ttgatgcgcc cgacttcaat attactcttg aaggtaacct
    45721 caaaaagcag ggtatcaaaa ccattcatta cgtcagtcca tccgtctggg cgtggcgaca
    45781 gaaacgcgtt ttcaaaatag gcagagccac cgatctggtg ctcgcatttc tgcctttcga
    45841 aaaagcgttt tatgacaaat acaatgtacc gtgccgcttt atcggtcata ccatggctga
    45901 tgccatgccg ttagatccag ataaaaatgg tgcccgtgat gtgctgggga tcccttacga
    45961 tgcccactgt ctggcattgt tgccgggcag ccgtggtgca gaagtcgaaa tgcttagtgc
    46021 cgatttcttg aaaactgccc agcttttgcg ccagacatat ccggatctcg aaatcgtggt
    46081 gccgctggtg aatgccaaac gccgcgagca gtttgaacgc atcaaagctg aagtcgcgcc
    46141 agacctgtcg gttcatttgc tggatggaat gggccgtgag gcgatggtcg ccagcgatgc
    46201 ggcactactg gcgtcgggga cggcagccct ggagtgtatg ctggcgaaat gcccgatggt
    46261 ggtgggatat cgcatgaagc cttttacctt ctggttggcg aagcggctgg tgaaaactga
    46321 ttatgtctcg ctgccaaatc tgctggcggg cagagagtta gtcaaagagt tattgcagga
    46381 agagtgtgag ccgcaaaaac tggctgcggc gctgttaccg ctgttggcga acgggaaaac
    46441 cagccacgcg atgcacgata ccttccgtga actgcatcag cagatccgct gcaatgccga
    46501 tgagcaggcg gcacaagccg ttctggagtt agcacaatga tcgaatttgt ttatccgcac
    46561 acgcagctgg ttgcgggtgt ggatgaagtc ggacgcgggc cgttagttgg cgcggtcgtc
    46621 accgctgcgg tgatccttga cccggcgcgc ccgattgccg ggctgaatga ttccaaaaag
    46681 ctgagcgaaa aacgccgtct ggcgctctat gaagagatca aagagaaagc gttgagctgg
    46741 agtctgggcc gcgcggaacc ccacgaaatc gacgagctga acattcttca tgcgaccatg
    46801 ctggcgatgc agcgtgccgt cgctgggctg catattgcgc cggaatatgt gttgattgat
    46861 ggtaaccgct gcccgaaatt accgatgcct gcgatggctg tggtgaaagg ggatagccgc
    46921 gtaccagaaa tcagtgccgc gtctatccta gcgaaagtga cgcgtgacgc cgaaatggcg
    46981 gcgctggata ttgttttccc gcaatatggt tttgcccaac ataaagggta tccaaccgct
    47041 tttcatctgg aaaaactggc tgaatatggc gcgactgaac atcatcgtcg cagctttggg
    47101 cctgtcaaac gcgcactggg acttgcgtcc tgattcttgt gtcgagatta agtaaaccgg
    47161 aatctgaaga tgtctgaacc acgtttcgta cacctgcggg tgcacagcga ctactcgatg
    47221 atcgatggct tggccaaaac cgcaccgctg gtaaaaaagg cggcggcgtt gggtatgcca
    47281 gcactggcga tcaccgattt caccaacctt tgcggtctgg tgaagttcta cggagcggga
    47341 catggcgcag ggattaagcc tatcgtcggg gcagatttta atgtccagtg cgacctgctg
    47401 ggtgatgagt taacccacct gacggtactg gcggcgaaca ataccggcta tcagaatctg
    47461 gcgttgctga tctcaaaagc gtatcagcgc gggtacggtg ccgccgggcc gatcatcgat
    47521 cgcgactggc ttatcgaatt aaacgaaggg ttgatccttc tttccggcgg gcgcatgggc
    47581 gacgtcggac gcagtctttt gcgtggtaac agcgcgctgg tagatgagtg tgtcgcgttt
    47641 tatgaagaac acttcccgga tcgctatttt ctagagctga tccgcaccgg caggccggat
    47701 gaagaaagct atctgcacgc ggcggtggaa ctggcggaag cgcgcggttt gcccgtcgtg
    47761 gcgactaacg atgtgcgctt tatcgacagc agcgactttg acgcacacga aatccgcgtc
    47821 gcgatccacg acggctttac cctcgacgat cctaaacgcc cgcgtaacta ttcgccgcag
    47881 caatatatgc gtagcgaaga ggagatgtgt gagctgtttg ccgacatccc agaagccctt
    47941 gccaacaccg ttgagatcgc caaacgctgt aacgtaactg tgcgtcttgg tgaatacttc
    48001 ctgccgcagt tcccgaccgg ggacatgagc accgaagatt atctggtcaa gcgtgcaaaa
    48061 gaaggcctgg aagagcgtct ggcattttta ttccctgacg aggaagaacg tcttaaacgc
    48121 cgcccggaat atgacgaacg tctggagact gaacttcagg ttatcaacca gatgggtttc
    48181 ccgggctact tcctcatcgt tatggaattt atccagtggt cgaaagataa cggcgtaccg
    48241 gtaggaccag gccgtggctc cggtgcgggt tcactggtgg cctacgcgct gaaaatcacc
    48301 gacctcgatc cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc ggaacgtgtc
    48361 tccatgcctg acttcgacgt tgacttctgt atggagaaac gcgatcaggt tatcgaacac
    48421 gtggcggaca tgtacggtcg tgatgcggta tcgcagatta tcaccttcgg tacgatggcg
    48481 gcgaaagcgg tgatccgcga cgtaggccgc gtgctggggc atccgtacgg ctttgtcgac
    48541 cgtatctcga aactgatccc gcccgatccg gggatgacgc tggcgaaagc gtttgaagcc
    48601 gagccgcagc tgccggaaat ctacgaagcg gatgaagaag ttaaggcgct gatcgacatg
    48661 gcgcgcaaac tggaaggggt cacccgtaac gccggtaagc acgccggtgg ggtggttatc
    48721 gcgccgacca aaattaccga ttttgcgccg ctttattgcg atgaagaggg caaacatccg
    48781 gtcacccagt ttgataaaag cgacgttgaa tacgccggac tggtgaagtt cgacttcctt
    48841 ggtttgcgta cgctcaccat catcaactgg gcgctggaga tgatcaacaa gcggcgggcg
    48901 aagaatggcg agccaccgct ggatatcgcc gcgatcccgc tggatgacaa gaaaagcttc
    48961 gacatgctgc aacgctcgga aaccacggcg gtattccagc ttgaatcgcg cggcatgaag
    49021 gacctgatca agcgtctgca acctgactgc ttcgaagata tgatcgcact ggtggcactg
    49081 ttccgccctg gtccgttgca gtcagggatg gtggataact ttatcgaccg taaacatggt
    49141 cgtgaagaga tctcctatcc ggacgtgcag tggcagcatg aaagcctgaa atcggtactg
    49201 gagccaacct acggcattat cttgtatcag gaacaggtca tgcagattgc ccaggtgctt
    49261 tctggttata ccctcggtgg cgcggatatg ctgcgccgtg cgatgggtaa gaaaaagccg
    49321 gaagagatgg ctaagcagcg ttctgtattt gctgaaggtg cagaaaagaa cggaatcaac
    49381 gccgaactgg cgatgaaaat cttcgacctg gtggagaaat tcgcgggtta cggatttaac
    49441 aaatcgcact ctgcggccta tgctttggtg tcatatcaaa cgttatggct gaaagcgcac
    49501 tatccggcgg agtttatggc ggcggtaatg accgccgata tggacaacac cgagaaggtg
    49561 gtgggcctgg tggatgagtg ctggcggatg gggctgaaaa tcctgccacc agatataaac
    49621 tccggtcttt accatttcca cgtcaacgac gacggcgaaa tcgtgtatgg tattggcgcc
    49681 atcaaagggg taggtgaagg tccgattgag gccatcatcg aagcccgtaa taaaggcggc
    49741 tacttccgcg aactgtttga tctctgcgcc cgtaccgaca ccaaaaagtt aaaccggcgg
    49801 gtgctggaaa aactgatcat gtccggggcg tttgaccgtc ttgggccaca ccgcgcggcg
    49861 ctgatgaact cgctgggtga tgcgttaaaa gcggcagatc aacacgcgaa agcggaagct
    49921 atcggtcagg ccgatatgtt cggcgtgctg gccgaagagc cggaacaaat tgaacaatcc
    49981 tacgccagct gccaaccgtg gccggagcag gtggtattag atggggaacg tgaaacgtta
    50041 ggcctgtacc tgaccggaca ccctatcaac cagtatttaa aagagattga gcgttatgtc
    50101 ggaggcgtaa ggctgaaaga catgcacccg acagaacgtg gtaaagtcat cacagctgcg
    50161 gggctcgttg ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat cggtatctgc
    50221 acgctggatg accgttccgg gcggctggaa gtgatgttgt ttactgacgc cctggataaa
    50281 taccagcaat tgctggaaaa agaccgcata cttatcgtca gcggacaggt cagctttgat
    50341 gacttcagcg gtgggcttaa aatgaccgct cgcgaagtga tggatattga cgaagcccgg
    50401 gaaaaatatg ctcgcgggct tgctatctcg ctgacggaca ggcaaattga tgaccagctt
    50461 ttaaaccgac tccgtcagtc tctggaaccc caccgctctg ggacaattcc agtacatctc
    50521 tactatcaga gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg gcgtgtctct
    50581 ccgagcgatc gtttattaaa cgatctccgt ggcctcattg gttcggagca ggtggaactg
    50641 gagtttgact aatacaggaa tactatgagt ctgaatttcc ttgattttga acagccgatt
    50701 gcagagctgg aagcgaaaat cgattctctg actgcggtta gccgtcagga tgagaaactg
    50761 gatattaaca tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga actgacgcgt
    50821 aaaatcttcg ccgatctcgg tgcatggcag attgcgcaac tggcacgcca tccgcagcgt
    50881 ccttataccc tagattacgt tcgcctggca tttgatgaat ttgacgaact ggctggcgac
    50941 cgtgcgtatg cagacgataa agctatcgtc ggtggtatcg cccgtctcga tggtcgtccg
    51001 gtgatgatca ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg ccgtaacttt
    51061 ggtatgccag cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat ggctgaacgc
    51121 tttaagatgc ctatcatcac ctttatcgac accccggggg cttatcctgg cgtgggcgca
    51181 gaagagcgtg gtcagtctga agccattgca cgcaacctgc gtgaaatgtc tcgccttggc
    51241 gtaccggtag tttgtacggt tatcggtgaa ggtggttctg gcggtgcgct ggcgattggc
    51301 gtgggcgata aagtgaatat gctgcaatac agcacctatt ccgttatctc gccggaaggt
    51361 tgtgcgtcca ttctgtggaa gagcgccgac aaagcgccgc tggcggctga agcgatgggt
    51421 atcatcgctc cgcgtctgaa agagctgaaa ctgatcgact ccatcatccc ggaaccgctg
    51481 ggcggcgctc accgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca actgctggcg
    51541 gatctggccg atctcgatgt gttaagcact gaagatttaa aaaatcgtcg ttatcagcgc
    51601 ctgatgagct acggttacgc gtaattcgca aaagttctga aaaagggtca cttcggtggc
    51661 ccttttttat cgccacggtt tgagcaggct atgattaagg aaggattttc caggaggaac
    51721 acatgaacat cattgccatt atgggaccgc atggcgtctt ttataaagat gagcccatca
    51781 aagaactgga gtcggcgctg gtggcgcaag gctttcagat tatctggcca caaaacagcg
    51841 ttgatttgct gaaatttatc gaacataacc ctcgaatttg cggcgtgatt tttgactggg
    51901 atgagtacag tctcgattta tgcagcgata tcaatcaact taatgaatat ctcccgcttt
    51961 atgccttcat caacacccac tcgacgatgg atgtcagcgt gcaggatatg cggatggcgc
    52021 tctggttttt tgaatatgcg ctggggcagg cggaagatat cgccattcgt atgcgtcagt
    52081 acaccaacga atatcttgat aacattacgc cgccgttcac gaaagccttg tttacctacg
    52141 tcaaagagcg gaagtacacc ttttgtacgc cggggcatat gggcggcacc gcatatcaaa
    52201 aaagcccggt tggctgtctg ttttatgatt ttttcggcgg gaataccctt aaggctgatg
    52261 tctctatttc ggtcaccgag cttggttcgt tgcttgacca caccgggcca cacctggaag
    52321 cggaagagta catcgcgcgg acttttggcg cggaacagag ttatatcgtt accaacggga
    52381 catcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt acgctgttga
    52441 tcgaccgcaa ctgccataaa tcactggcgc atctgttgat gatgaacgat gtagtgccag
    52501 tctggctgaa accgacgcgt aatgcgttgg ggattctggg ggggatcccg cgccgtgaat
    52561 ttactcgcga cagcatcgaa gagaaagtcg ctgccactac gcaagcacaa tggccggttc
    52621 atgcggtgat caccaactcc acctatgatg gtttgctcta caacaccgac tggatcaaac
    52681 agacgctgga tgtcccgtcg atccacttcg attccgcctg ggtgccgtat actcattttc
    52741 atccaatcta ccagggtaaa agtggtatga gcggcgagcg tgttgcggga aaagttttct
    52801 tcgaaacgca atcgacccac aaaatgctgg cggcgttatc gcaggcttca ctgatccaca
    52861 ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt tatgatgcat accaccacct
    52921 cgcccagtta tcccattgtt gcttcggttg agacggcggc ggcgatgctg cgtggtaatc
    52981 cgggcaaacg gctgattaac cgttcggtgg agcgggcgct gcattttcgc aaagaggtcc
    53041 agcggctgcg ggaagagtct gacggttggt ttttcgatat ctggcaaccg ccgcaggtgg
    53101 atgaagccga atgctggccc gttgcgcctg gcgaacaatg gcacggcttt aacgatgcgg
    53161 atgccgatca tatgtttctc gatccggtta aagtcactat tttgacaccg gggatggacg
    53221 aacagggcaa tatgagtgag gaggggatcc cggcggcgct ggtagcaaaa ttcctcgacg
    53281 aacgtgggat cgtagtagag aaaaccggcc cttataacct gctgtttctc tttagtattg
    53341 gcatcgataa aaccaaagca atgggattat tgcgtgggtt gacggaattt aagcgctctt
    53401 acgatctcaa cctgcggatc aaaaatatgc tgcccgatct ctatgctgaa gatcccgatt
    53461 tctaccgcaa tatgcgtatt caggatctgg cgcaagggat ccataagctg attcgtaaac
    53521 acgatctttc cggtttgatg ttgcgggcat tcgatacttt gccggaaatg atcatgacgc
    53581 cacatcaggc atggcagaga cagattaaag gtgaagtaga aaccattgcg ctggaacaac
    53641 tggtcggtag agtatcggca aatatgatcc tgccttatcc accgggcgta ccgctgttga
    53701 tgccaggaga aatgctgacc aaagagagcc gcacggtact cgattttcta ctgatgcttt
    53761 gttccgtagg gcaacattac cccggttttg aaacggatat tcacggcgcg aaacaggacg
    53821 aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc agagcggctt
    53881 ctgggcgagt aacgtgctgt taacaaataa aggagacgtt atgctgggtt taaaacaggt
    53941 tcaccatatt gcgattattg cgacggatta tgcggtgagc aaagctttct actgcgatat
    54001 tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt ggaaagggga
    54061 tttggcgctt aatgggcaat atgtgattga gcttttctct tttccgttcc cgccggagcg
    54121 ccccagccga ccggaagctt gcggcctgcg tcatctggct tttagcgttg atgacatcga
    54181 tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc gtgtcgatcc
    54241 atacacgcaa aaacgcttca ccttctttaa cgatccggac gggctgccgt tggaactgta
    54301 tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac gtgccgggca ttgctactgt
    54361 aaaatcgcac catcatgaca ctcacgctca atagaccact tctcacctca cgccagattc
    54421 tggtggcctt tagcggcggg cttgactcca ccgttctgct gcatcagttg gtgcagtggc
    54481 ggacggaaaa tccgggcgtc actctgcgcg ctattcatgt gcatcacggt ttaagtgcca
    54541 atgccgatgc ctgggttacg cattgcgaaa acgtctgcca acagtggcag gtgccgctgg
    54601 tggtcgaacg cgtacaactt gcgcaagagg ggctggggat tgaggcccag gcgcggcagg
    54661 cgcgttatca ggcatttgcc cgtaccctat tgcccggcga agtgctggtc accgcgcaac
    54721 atctcgacga tcaatgtgaa acctttctgc tggcgctaaa acgcggtagc ggccctgccg
    54781 ggctttcggc tatggcggaa gtttcggagt ttgctggaac acggcttatc cgcccgttgc
    54841 ttgcccgcac gcggggggaa ctggagcagt gggcattggc gcatggttta cgctggattg
    54901 aagacgaaag taatcaggac gactcatacg atcgtaactt tctgcgcctg cgcgtagtgc
    54961 cgttattgca gcagcgttgg ccgcattttg cggaagcaac ggcccgcagc gccacacttt
    55021 gtgctgaaca agagagtctg ttggatgaac tgctggcgga tgatttagca cactgccaaa
    55081 cgtcgcaggg gacgctgcag attgcgccaa tgctggcgat gagtgatgcc cgtcgcgcgg
    55141 cgattattcg ccgctggctg gcagggcaga atgcaccgat gccttcccgc gacgcgctgg
    55201 tgcgcatctg gcaggaagtg gcgctggcgc gggaagatgc ttccccctgt ttacgtttgg
    55261 gcgcctttga aatccgccgc tatcagtcgc aactgtggtg gattaaatcc gtcaccgggc
    55321 aaagcgaaac cattgtgctg tggcagacgt ggctacaacc gctggaatta ccggcggggc
    55381 tgggaaccgt acagcttact gcgggaggcg atattcgccc tccgcgtgca gacgaagcgg
    55441 tcagcgtgcg tttcaaagcg ccaggattgc tgcatattgt cgggcgcaac ggcggacgta
    55501 agctgaagaa aatctggcaa gagttaggcg tgccgccgtg gctacgtgac accacgccac
    55561 tgctgtttta tggagaaacg ctgattgcgg cggcaggggt atttgtgacg caagaaggtg
    55621 tggctgaagg tgagaatggc gtaagttttg tctggcagaa aacgcttagt taagtgaaag
    55681 ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca atcaggattc gcttaccacc
    55741 accgtaccga tttcagggtg gctaaagctg gtaattttat ccagacgcag ctcgcgggtt
    55801 tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa atcactggct
    55861 tttgcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg atgctggcag
    55921 gcgagctcaa gattatcgta atcatcacaa ttaattggtt gatacgtatc attcattgac
    55981 ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga cagcctcaga
    56041 aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca gcgcgtccgg
    56101 aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta actcacaata
    56161 tttttccaca tgccctcctg tcagcactct gacttaaccg tggatgcaag tctaagccta
    56221 cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca atatcagcgc
    56281 cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt tcttatggcg
    56341 cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg caaccagttt
    56401 ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg tatgatgctg
    56461 cgcttgctgg cgtggctgaa atatgctgat gaacgtctgc aatttacccg tggtttgtgt
    56521 gccgatgatg agccggaagc gtggctgcgt aacgatcacc tgggcattga tttgtggatt
    56581 gagctggggc tgccggatga gcgacgaatt aagaaagcct gcacccaggc cgcagaagtg
    56641 gcgctgttta cctataatag tcgggcggcg caaatctggt ggcagcaaaa tcagagcaaa
    56701 tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg atgaacaact ggcgaaagta
    56761 agcgcctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg tgttatctgg
    56821 ttatcggatg ataagaataa tctggaagtt aacttaaccg cctggtaaca accttcatga
    56881 ttgtgatttc ccgacatgtt gctattcccg atggtgagct tgagatcacc gccattcgtg
    56941 cgcagggcgc tggcgggcag catgttaata agacctcaac ggctattcat ctgcgttttg
    57001 acattcgggc gcccagcctg ccagagtatt acaaagagcg tctgctcgcc gccagccatc
    57061 atttgatcag cagtgatggc gtgattgtca ttaaggcaca ggaataccgc agtcaggaac
    57121 tgaaccgcga agccgctctg gcccggttgg tggcagtgat taaagattta acaaaagaac
    57181 aaaaagcccg acgacccacg cggcccaccc gtgcatcgaa agagcgcagg ctggcatcga
    57241 aagcacaaaa atcaagcgtg aaggcgatgc gcggcaaagt acgcagcggt cgggaataaa
    57301 aagaaggaat ggatggtgaa aaaagcgata gtgacagcga tggctgtaat cagcctcttt
    57361 actctgatgg gatgtaataa tcgggccgaa gtcgatacgc tttctccggc gcaggctgcc
    57421 gaactgaaac cgatgccgca aagttggcgc ggcgtgctac cgtgtgccga ttgcgaagga
    57481 atcgaaatct ctctgttcct cgaaaaagac ggaacatggg tgatgaatga gcgttatctc
    57541 ggtgctcgtg aagaaccttc ctccttcgct tcctacggta catgggcgcg aaccgctgac
    57601 aagctggtat taaccgacag caaaggtgaa aagtcatatt atcgggcgaa aggcgatgcg
    57661 ctggagatgc tcgatcgtga aggcaatcca attgaatcgc agttcaacta tacgctggaa
    57721 ccggcacaat ccagtttacc tatgacgccg atgaccctgc gaggcatgta tttttatatg
    57781 gctgatgcgg cgaccttcac tgattgcgcg accggaaaac gtttcatggt agcgaataac
    57841 gcagagctgg agcgtagcta cctggctgcg cgcggtcaca gtgaaaaacc ggtgttactg
    57901 tcagtagaag gtcactttac gcttgaggct aatccggata ctggtgcgcc aaccaaagta
    57961 ttagcgcccg atacggcggg taaattttac ccgaataagg attgcagtag tttggggcag
    58021 taaacctgct taaggcagaa acaaacgcaa aattgcctga tgcgctgcgc ttatcaggcc
    58081 tacagcatat aatgcaacat tttgaattag cacgattttg taggccggat aaggcgttta
    58141 cgccgcatcc ggcattttgt gcacgaggct ggctttatca ctgagtcagc ccaacaaagc
    58201 gacccgcctt gatataaatc ccaggcttca ggtgcccaac atactgtaac tgcgtttctg
    58261 gcttaaacgc agagacatca ccaatacgca tatgcatcag atcggcaggg ctaatccagc
    58321 ccgattgcgc cagtgtaagt ggatgcccgg ctttggcaaa tgcatcagtg acaaattccg
    58381 aacaaaacca cgactttttg tctccttcgc acacactgct taactgcgct ttcgccaggt
    58441 cgctgacgca ctgttggcga aaatcctcgg agaacggatt cagtgagcac atctggcgag
    58501 tcaccataaa gggaataaat tcgacaatgt tgcgatagtt ataaccgcta tctttgattt
    58561 tattggcaaa cgcggtgata tctgtggctt gttgcggggt aagatccggg actcgtaaga
    58621 cgaaaagctt atcactatgc ttcatcgctt ttttaaggga aacaatctgg acgccagcgc
    58681 ctgtcgcttc tgcaacgtta ttatcaccca gaaagattgc aacgtgactc acagaggaag
    58741 tgctgaagac gcggattcca aatgaggtta ccccaaggct tgaggagaac agcaaatcgc
    58801 cgggtttgag atcaggtgcc gttatttctt ttattgattg ttcggtgaaa gagctttgat
    58861 gctggaattt gacagcccat gtttttgcct cggcatctac tgctgtggct gatgggtctg
    58921 gctggctgat atcaacggta caagccgaaa gtaaaagaaa gctggggaga agcaggcggc
    58981 agtacgcctt tggtttatcc attttataca atccatgtaa aaaagggccc tgaaattcag
    59041 gaccctttct ggcatcagcc tttaatctgt ttcaccagat attcgacgat gtcaccagtc
    59101 ttaattaact gtttctcgcc gttgcgacga tatttatatt cgatatcgtc gttgtcgagg
    59161 ttacggtcgc ccagcacaat agtgtgcgga ataccgatca gttccatatc agcaaacatc
    59221 acgcccggac gctctttgcg gtcatccagc agcacttcga taccttgtgc acgcagttcg
    59281 ctgtacagtt tctcagcaag ctcttgtacg cggaaggatt tgtgcatgtt catcggcaga
    59341 atcgccacct ggaacggcgc gatagcgtca ggccatacga tgccgcgttc gtcgtagttc
    59401 tgctcaatcg ccgcagctac cacacgcgtt accccgatac cgtagcaacc catcgtcagg
    59461 atttggttac ggccatcttc accctgtacg gaggctttca gtgcttcgga gtacttggta
    59521 cccagctgga agatgtgacc aacttcgata ccacgtttga tcagcagcgt accctggcca
    59581 tccgggcttg gatcgccagc caccacgtta cggatatctg caacttccgg ggtagcgaca
    59641 tcgcgatccc agttgatacc gaagtagtgt ttaccatcga tgttagcacc agcagcgaaa
    59701 tcactcatcg ccgcaacggt acggtcaatc accaccggaa tcggcatgtt taccggaccc
    59761 agtgaacccg gaccggcttt aaccacagca cgaatttctt cttcggtcgc gaaagtcagc
    59821 gggcttgcaa cctgcggcag tttttctgct ttaacttcgt tcagctcgtg atcaccgcgc
    59881 accagcagcg caaccagcgg gaagctactg ccttcaaccg ctttaaccag cagagtctta
    59941 accgttttct caatcggcag attgaactgt tcaaccagtt ccgcgatggt tttcgcgttc
    60001 ggcgtatcaa ccagcgtcat ttcctgggta gcagcagcgc gcggttcttt cggcgcgata
    60061 gcttctgcca gttcaatgtt cgctgcatag tcagaggtgt cggagaagac cacatcgtct
    60121 tcaccgctct gcgccagcac ctggaattcg tgagaggcgc tgccgccgat agaaccggtg
    60181 tcggcctgta cggcgcggaa atccagcccc atgcggctga agattttgct gtaggccgca
    60241 tacattgcat cgtaggtttc ctgcagggat tcctgagaag tatggaaaga gtaagcatct
    60301 ttcatcagga attcgcggga acgcatgacg ccgaaacgcg gacgcacttc gtcgcggaac
    60361 ttggtctgga tctgatagaa gttcagcggc agctgtttgt aagagctaag ctcgttacga
    60421 atcaggtcag tgataacttc ttcatgagtt gggccgagta cgaacggacg ctcgccacgg
    60481 tcaacaaaac gcagcagttc cggaccgtac tgttcccaac gaccactctc ttgccacaaa
    60541 tcggctggct gaaccaccgg catcgacacc tcgatcgcac cggcgttgtt catctcttca
    60601 cgcacgatgt tttcgacttt tttcagaacg cgcacgccgg tcggcagcca ggtatataac
    60661 ccggaggcca gcttgcggat catcccggcg cgcagcatca gctgatggct gatcacctcg
    60721 gcgtcggcag gtgtctcctt gagagtggag agcaggtatt ggctagtacg catgttgtta
    60781 cggttccagt tggaaggtag aacaggctca aggcgagcct gggacaaaaa aagtgattta
    60841 gtttaccagt gcaaaagaaa tgtcaaaaga gaagggcgtg aatttaacgc ggttccagcg
    60901 caaagacttc aaaacctgcg tcggtgacgc gccagcgaac gttaaaatca tgtagccaga
    60961 cggcataggt tttgcccgtt tcctcacctt tacgataggc cgggcgcggg tcctgcgcca
    61021 gtacttcgcg gataaacagc gttaactgcg gataacgctt ctccagcgtc aaaagctgct
    61081 tttcgacctc tgcggtaaaa ctcaccgcca tctctgcagc tggcgcactt tgcgcatagc
    61141 tggcactggc atcgggaagc gattcggcaa aggggagata cggtttgata tccactaccg
    61201 gcgtaccatc gaccagatcc agactgccga gcttcagaat cacgctgtct ttatggcaaa
    61261 caacctcttt cagctctacc agcgacatgc caattgggtt agggcggaaa gtagagcgtg
    61321 ttgcgaaaac ccccattctg gcgttaccgc cgaggcgcgg tggacgcaca gtcggacgcc
    61381 agccgccttc catcgtttga tgaaagacga aaaggatcca taaatggctg aatgcttcca
    61441 ggccgcgaac ggcgtcggcc tggttgtagg aagcaatgag atgcagttcg ccgttggcgc
    61501 tttttaccag acctggctgg cgcggaacgg cgaatttttc tttatagggc gagcgaataa
    61561 cgcctatttg ctcgaactgg aaactgctca tttcgccgta atgttaagcg cagagccgat
    61621 acatacagcc tgacgatagc agcccggcgt accgctggtg acttcgcagc tatgcagtaa
    61681 tacagcattg gctttcattt tagaggcatt gatttgcatc tgcttacgtg cggttggaat
    61741 gctcggcgga gagtcctgat tagaggcctg gcaagagtcg ccactgactt caccgagatc
    61801 gcggaacggt ttgccgacta attcttctgc attggtataa attcggaccg gcgtggcgcg
    61861 cggcgctttc ggttttgcag gctccgcttt cggctggggt gcagtgcttt gaacgggttc
    61921 gacaggggat ctgcttaaca tggaacagcc gcttagcatg agtgctacta aacagatcgg
    61981 taaagcacgc atagtatttc ctcaatgtat gatcaaaacg tcaatattga atcaggagct
    62041 tgtaaaaatg acaagacggg caagcgcccg tcctgaatga tattacaaat tgtggaaaca
    62101 gcctaaaaat taccagcctt taacagcgcc gccgttaaac actttgtttg ctgcttcgta
    62161 aacttcatca gactgataag cctggacgaa tttcttcacg ttctcggcgt ctttgttatc
    62221 ttcacgcgtt acgatcaggt ttacgtatgg ggagtcttta tcttcgacaa agataccgtc
    62281 tttcgccgga gtcaggccaa tctggctggc ataggtggta ttgataactg ccagagcgat
    62341 ttgcgcgtcg tccagagaac gcggcagttg cggtgcttcc agttcaacaa ttttcagatt
    62401 ttttgggttc tcaacaacat caagaactgt cggcagcagg ccaacgccat ctttcagttt
    62461 gatcaagccc actttttgca gcagcagcag tgaacgacca agattagttg gatcgtttgg
    62521 cacggcaacc tgcgaaccat cctgcagttc atccagtgat ttgattttct tggagtaacc
    62581 agcaatcgga taaacaaaag tgttgcctac tgcgaccagt ttgtagccac gatctttcag
    62641 ttgctgatca aggtacggtt tatgctggaa ggcgttggcg tcgatatcgc ctttgctcaa
    62701 tgcttcgttt ggcagaacat agtcgttgaa ggttaccagc tcaacgtcca ggccatattt
    62761 gtctttcgca actttctgcg cgacttctgc tacctgctgt tcggcaccaa caatcacgcc
    62821 gactttaatg tggtttggat ctttttcatc ctgaccgcag cctaccagtg ccagtgatcc
    62881 aatcagggct cccactgccg caaaggtttt gaatttgaac gccatacctt attccttctt
    62941 caattattta tgttgtgttg aacgttactt gcgagtgaca gcccggacga tgcggtcgcc
    63001 tgcgaactga attaaataaa ccagaatgac cagcaatacc agtaccgtat tcatcaccgt
    63061 cgcgttgtag ccgatgtagc catactgata gccaatctga cctaaaccac cggcaccgac
    63121 tgcaccaccc atcgcggaat aaccgaccag ggtaatcagg gtgatagttg ccgcattcac
    63181 cagacccggc aacgcttccg gtaacagcac tttacggacg atctgcattg gcgtggcacc
    63241 cattgcgcgg gaagcttcaa ttaacccggt tgggatctcc agcagagcgt tctcgaccat
    63301 acgggcaata aacggtgctg caccaacggt taacggaaca attgctgcct gcaaaccaat
    63361 cgatgtaccg acaataacgc gggtaaacgg aatcatccat accagcaaga taatgaacgg
    63421 gatggaacgg aaaatgttca caatcgcaga aacggtacga tacagcttcg cgttagcaat
    63481 aatttgcccc ggacgcgtga cataaagcag aacgccaacc ggcagaccaa tcacaaaccc
    63541 aaaaaatccg gatacgaagg tcattgccag cgtttcccat acgccacgaa ccagcagcca
    63601 catcatcggc tcagacataa cccagtacct ctacttttac atggtgttcc tgcagccagg
    63661 caatggcggc ttgcgtatct tgttgtgtgc cgtgcatttc agtcagcatg atgccgaatt
    63721 taacgccacc ggcgtagtcc atctgcgcgc taataatgtt gttgttgacg ttgaaacgac
    63781 gcgcggtttc agaaagcagt ggggcatcga ccgattgacc ggtaaactcc agacgcagca
    63841 tcggcacgca gtcagtaaat ggctccgttt gcagacgttc ctggtaatct tccgggatat
    63901 ccagatgcag ggtcgactga ataaacttct gcgccagcgg cgttttcgga tgcgagaaca
    63961 cttcacttac cgtgtcctgc tcgatcagtt cgccattgct gatgaccgcc acgcaatcac
    64021 aaatacgctt cacaacgtcc atttcgtggg tgatcaacag aatcgtcaaa cccagacggc
    64081 ggttaatgtc tttcagcagt tcgagaatag aacgtgtcgt tgccgggtcc agcgcgctgg
    64141 tggcttcatc acacagcaat actttgggat tgctggctaa tgcacgggca atcgccacac
    64201 gttgtttctg cccaccggaa agattcgacg ggtagctatc atgcttatcg ccaagaccaa
    64261 ccaatgacag caattccgtc acgcgacgtt tgatctcgtc tttcggtgtg ttgtccagct
    64321 ccagcggcag agccacgttg ccaaaaacag tacgcgaaga gagcaggtta aaatgctgga
    64381 aaatcatacc aatctggcgg cgagctttgg tcaactcgga ttctgacagc gtggtcaatt
    64441 cctggccatc gaccagcacg ctaccctcgg ttgggcgctc cagcaggttt acacaacgta
    64501 taagcgtact cttacccgcg cctgaggcac cgataacgcc ataaatttgt ccagctggca
    64561 catgcaggct gacgttgttc aacgcctgga tggtgcgggt gccctggtgg aacactttgg
    64621 tgatattcga aagttttatc attgattatt tattatcgtc attaagttag tcgtagcatc
    64681 tcgaatgcct gaaacgggca acgccgtcaa tgaaatggat gttaaggcat ccagacgtct
    64741 aaatcaatca ggtttatgcg aagagcactt tcttgcaggt cgaaacatgc gatactagcg
    64801 tcccatgcct tattaaggag ctataaaagg tggcgaagag cgtacccgca atttttcttg
    64861 accgtgatgg caccattaat gtcgatcacg gctatgtcca tgagatcgac aactttgaat
    64921 ttatcgacgg tgttattgac gccatgcgcg agctaaaaaa aatgggcttt gcgctggtgg
    64981 tagtaaccaa ccagtctggc attgctcgcg gtaaatttac cgaagcacag tttgaaacgc
    65041 tgaccgagtg gatggactgg tcgctggcgg accgagatgt cgatctggat ggtatctatt
    65101 attgcccgca tcatccgcag gggagtgttg aagagtttcg ccaggtctgc gattgccgca
    65161 aaccacatcc ggggatgctt ttgtcagcac gcgattattt gcatattgat atggccgctt
    65221 cttatatggt gggcgataaa ttagaagata tgcaggcagc ggttgcggcg aacgtgggaa
    65281 caaaagtgct ggtgcgtacg ggtaaaccta ttacacctga agcagaaaac gcggcagatt
    65341 gggtgttaaa tagcctggca gacctgccgc aagcgataaa aaagcagcaa aaaccggcgc
    65401 aatgattaaa agatgaacgg ttgaaataaa aatgcatttt tccgcttgtc ttcctgagcc
    65461 gactccctat aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc
    65521 ggttcggttg aagagaaaaa atcctgaaat tcaggggtga ctctgaaaga ggaaagtgtg
    65581 atatacgcca cctcgcgaca gtgagctgaa agc
//
LOCUS       DAJVQH010000008        65498 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJVQH010000008 DAJVQH010000000
VERSION     DAJVQH010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.008"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            132..575
                     /locus_tag="OQM87_000599"
     CDS             132..575
                     /locus_tag="OQM87_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1506857.1"
                     /translation="MDLALVTGQREGDLAAMRWVDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1630
                     /gene="ybhA"
                     /locus_tag="OQM87_000600"
                     /pseudo
     CDS             811..1630
                     /gene="ybhA"
                     /locus_tag="OQM87_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="OQM87_000601"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="OQM87_000601"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCS1506858.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTCIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="OQM87_000602"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="OQM87_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCS1506859.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="OQM87_000603"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="OQM87_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1506860.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="OQM87_000604"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="OQM87_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCS1506861.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="OQM87_000605"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="OQM87_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCS1506862.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="OQM87_000606"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="OQM87_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCS1506863.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="OQM87_000607"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="OQM87_000607"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCS1506864.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="OQM87_000608"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="OQM87_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCS1506865.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="OQM87_000609"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="OQM87_000609"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCS1506866.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="OQM87_000610"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="OQM87_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCS1506867.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="OQM87_000611"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="OQM87_000611"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCS1506868.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="OQM87_000612"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="OQM87_000612"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCS1506869.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13936..14316
                     /locus_tag="OQM87_000613"
     CDS             13936..14316
                     /locus_tag="OQM87_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCS1506870.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="OQM87_000614"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="OQM87_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCS1506871.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="OQM87_000615"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="OQM87_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCS1506872.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="OQM87_000616"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="OQM87_000616"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS1506873.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
     gene            complement(17417..17492)
                     /locus_tag="OQM87_000617"
     tRNA            complement(17417..17492)
                     /locus_tag="OQM87_000617"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="OQM87_000618"
     tRNA            complement(17496..17571)
                     /locus_tag="OQM87_000618"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="OQM87_000619"
     tRNA            complement(17623..17698)
                     /locus_tag="OQM87_000619"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="OQM87_000620"
     tRNA            complement(17701..17776)
                     /locus_tag="OQM87_000620"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17913..17988)
                     /locus_tag="OQM87_000621"
     tRNA            complement(17913..17988)
                     /locus_tag="OQM87_000621"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17953..17955),aa:Lys,seq:ttt)
     gene            complement(18153..18944)
                     /gene="cpoB"
                     /locus_tag="OQM87_000622"
     CDS             complement(18153..18944)
                     /gene="cpoB"
                     /locus_tag="OQM87_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCS1506874.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18954..19475)
                     /gene="pal"
                     /locus_tag="OQM87_000623"
     CDS             complement(18954..19475)
                     /gene="pal"
                     /locus_tag="OQM87_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCS1506875.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19510..20802)
                     /gene="tolB"
                     /locus_tag="OQM87_000624"
     CDS             complement(19510..20802)
                     /gene="tolB"
                     /locus_tag="OQM87_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCS1506876.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20935..22191)
                     /gene="tolA"
                     /locus_tag="OQM87_000625"
     CDS             complement(20935..22191)
                     /gene="tolA"
                     /locus_tag="OQM87_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCS1506877.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAK
                     AAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADI
                     NNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAA
                     KLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22256..22684)
                     /gene="tolR"
                     /locus_tag="OQM87_000626"
     CDS             complement(22256..22684)
                     /gene="tolR"
                     /locus_tag="OQM87_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCS1506878.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22688..23380)
                     /gene="tolQ"
                     /locus_tag="OQM87_000627"
     CDS             complement(22688..23380)
                     /gene="tolQ"
                     /locus_tag="OQM87_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCS1506879.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23377..23781)
                     /gene="ybgC"
                     /locus_tag="OQM87_000628"
     CDS             complement(23377..23781)
                     /gene="ybgC"
                     /locus_tag="OQM87_000628"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1506880.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23931..24224)
                     /gene="ybgE"
                     /locus_tag="OQM87_000629"
     CDS             complement(23931..24224)
                     /gene="ybgE"
                     /locus_tag="OQM87_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCS1506881.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24224..24337)
                     /gene="cydX"
                     /locus_tag="OQM87_000630"
     CDS             complement(24224..24337)
                     /gene="cydX"
                     /locus_tag="OQM87_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCS1506882.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24352..25491)
                     /gene="cydB"
                     /locus_tag="OQM87_000631"
     CDS             complement(24352..25491)
                     /gene="cydB"
                     /locus_tag="OQM87_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1506883.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25507..27075)
                     /gene="cydA"
                     /locus_tag="OQM87_000632"
     CDS             complement(25507..27075)
                     /gene="cydA"
                     /locus_tag="OQM87_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCS1506884.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27924..30557)
                     /gene="mngB"
                     /locus_tag="OQM87_000633"
     CDS             complement(27924..30557)
                     /gene="mngB"
                     /locus_tag="OQM87_000633"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCS1506885.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKIWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30575..32491)
                     /gene="mngA"
                     /locus_tag="OQM87_000634"
     CDS             complement(30575..32491)
                     /gene="mngA"
                     /locus_tag="OQM87_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCS1506886.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWESVDGPEAIDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTVQQDT
                     QPVKSVKTELKQALLSGISFSVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMQ"
     gene            32661..33383
                     /gene="mngR"
                     /locus_tag="OQM87_000635"
     CDS             32661..33383
                     /gene="mngR"
                     /locus_tag="OQM87_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCS1506887.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33487..34356)
                     /gene="sucD"
                     /locus_tag="OQM87_000636"
     CDS             complement(33487..34356)
                     /gene="sucD"
                     /locus_tag="OQM87_000636"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCS1506888.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34356..35522)
                     /gene="sucC"
                     /locus_tag="OQM87_000637"
     CDS             complement(34356..35522)
                     /gene="sucC"
                     /locus_tag="OQM87_000637"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCS1506889.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35616..36833)
                     /gene="odhB"
                     /locus_tag="OQM87_000638"
     CDS             complement(35616..36833)
                     /gene="odhB"
                     /locus_tag="OQM87_000638"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCS1506890.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36848..39649)
                     /gene="sucA"
                     /locus_tag="OQM87_000639"
     CDS             complement(36848..39649)
                     /gene="sucA"
                     /locus_tag="OQM87_000639"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCS1506891.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39950..40666)
                     /gene="sdhB"
                     /locus_tag="OQM87_000640"
     CDS             complement(39950..40666)
                     /gene="sdhB"
                     /locus_tag="OQM87_000640"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCS1506892.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40682..42448)
                     /gene="sdhA"
                     /locus_tag="OQM87_000641"
     CDS             complement(40682..42448)
                     /gene="sdhA"
                     /locus_tag="OQM87_000641"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCS1506893.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42448..42795)
                     /gene="sdhD"
                     /locus_tag="OQM87_000642"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42448..42795)
                     /gene="sdhD"
                     /locus_tag="OQM87_000642"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCS1506894.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42789..43193)
                     /gene="sdhC"
                     /locus_tag="OQM87_000643"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42789..43193)
                     /gene="sdhC"
                     /locus_tag="OQM87_000643"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1506895.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43574..43681
                     /locus_tag="OQM87_000644"
     CDS             43574..43681
                     /locus_tag="OQM87_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506896.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43887..45170
                     /gene="gltA"
                     /locus_tag="OQM87_000645"
     CDS             43887..45170
                     /gene="gltA"
                     /locus_tag="OQM87_000645"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCS1506897.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45561..46127
                     /locus_tag="OQM87_000646"
     CDS             45561..46127
                     /locus_tag="OQM87_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1506898.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATCVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46178..48637
                     /locus_tag="OQM87_000647"
     CDS             46178..48637
                     /locus_tag="OQM87_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1506899.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALALEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGQVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48652..49383
                     /locus_tag="OQM87_000648"
     CDS             48652..49383
                     /locus_tag="OQM87_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1506900.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49380..50442
                     /locus_tag="OQM87_000649"
                     /pseudo
     CDS             49380..50442
                     /locus_tag="OQM87_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50594..51640
                     /locus_tag="OQM87_000650"
     CDS             50594..51640
                     /locus_tag="OQM87_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCS1506901.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51637..52428)
                     /gene="nei"
                     /locus_tag="OQM87_000651"
     CDS             complement(51637..52428)
                     /gene="nei"
                     /locus_tag="OQM87_000651"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCS1506902.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52464..53198)
                     /gene="pxpA"
                     /locus_tag="OQM87_000652"
     CDS             complement(52464..53198)
                     /gene="pxpA"
                     /locus_tag="OQM87_000652"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCS1506903.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53188..54120)
                     /gene="pxpC"
                     /locus_tag="OQM87_000653"
     CDS             complement(53188..54120)
                     /gene="pxpC"
                     /locus_tag="OQM87_000653"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCS1506904.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(54114..54770)
                     /gene="pxpB"
                     /locus_tag="OQM87_000654"
     CDS             complement(54114..54770)
                     /gene="pxpB"
                     /locus_tag="OQM87_000654"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCS1506905.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQQHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54793..55536)
                     /gene="ybgI"
                     /locus_tag="OQM87_000655"
     CDS             complement(54793..55536)
                     /gene="ybgI"
                     /locus_tag="OQM87_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCS1506906.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55807..57288
                     /gene="dtpD"
                     /locus_tag="OQM87_000656"
     CDS             55807..57288
                     /gene="dtpD"
                     /locus_tag="OQM87_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCS1506907.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57438..58856)
                     /gene="phrB"
                     /locus_tag="OQM87_000657"
     CDS             complement(57438..58856)
                     /gene="phrB"
                     /locus_tag="OQM87_000657"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCS1506908.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYETARKGK"
     gene            complement(58853..59362)
                     /locus_tag="OQM87_000658"
     CDS             complement(58853..59362)
                     /locus_tag="OQM87_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCS1506909.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59491..59808
                     /locus_tag="OQM87_000659"
     CDS             59491..59808
                     /locus_tag="OQM87_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCS1506910.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(59863..60243)
                     /locus_tag="OQM87_000660"
     CDS             complement(59863..60243)
                     /locus_tag="OQM87_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506911.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     EEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYTL"
     gene            complement(60246..64910)
                     /locus_tag="OQM87_000661"
     CDS             complement(60246..64910)
                     /locus_tag="OQM87_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1506912.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGGTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNTWRCVYDERGNLLAVTDPLQQNTRYQYDHHGQVVQ
                     ITDARGGNKYLQWNEDGQLMRHTDCSGSQTAWFYDERTRLIRMTDAQSHSTRYGYDDS
                     GHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQRDGQGRVRSRTDAMGRRTAF
                     GYDAYGRLVTLTNENGERYWFRHDVLDRLAEQINPDGCCQSYRYNALNAVTEVVFTGD
                     RGGEIRHRLARDAAGRLTAKETADGRTEYVHDAADQLLEIRRQRHETDAPEVIRFSYD
                     RLGRMLSEETAQGILTHQYDELGNRTATTFPDGRTQRHLYYGSGHLQQINLDREVISE
                     FTRDHLHREVLRSQGRLSTRQLYDPGGRLKRRETYSGMRGVVPETFTDRQYSYSGEDE
                     LLKTRHSRRGETDYFYDPTGSITACRSEDEGYLASWQYDAAGNLLGRRAGERATAENS
                     VVPFNRLMSYRGVHYRYDEFGRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYD
                     ALGRRTEKQHISPDGKPYNRTKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVD
                     KAGKEGPNRILYFHTDVNGAPEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRY
                     QGQYLDRETGLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTP
                     CAVSNQKANRLLDSSETKVTVRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYL
                     TGNKTKAGSYHWDDIKDPSVTKPPYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPR
                     ET"
     gene            complement(64929..65132)
                     /locus_tag="OQM87_000662"
                     /pseudo
     CDS             complement(64929..65132)
                     /locus_tag="OQM87_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15866 a        17744 c        15994 g        15894 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg cgaaggagat ctggcggcta
      181 tgagatgggt ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaatcgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg caccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 accacttgta atcaattgat tataaaggat taattcataa agagcattta cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcaccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgcag gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagtaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattaccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagct gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gatgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag atgacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggcc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgtct ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgccctct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagcgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggcgga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tataccaacc gcatcgccaa tagtcgttat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattacagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagtatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcgccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatga gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgtcgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctaga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctttcctaca atttctaact gtaattcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca cgccaccatt cagatggaat
    15301 atcaaccttg tcatgggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 accgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggcca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgtttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgcaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcgc attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttacattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtggtgggtc
    17821 gtgcaggatg actcgacttc gtctcgccct tcggcgccgt tgctgatgca acgttatcct
    17881 tcacatttta ctaccgcttc caccttagcg attggtgggt cgtgcaggat tcgaacctgc
    17941 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccac tttttcgttg
    18001 ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt tcagacttga
    18061 gcgcaacaaa aatttcgatg cagatcactc aactgcttat gattcgcacg acacgaccag
    18121 aaataatgcg acttctggtc gtgttttatg cattacatcg cgttcagacg tttttgtgcc
    18181 tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac ggctttcgct
    18241 tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc atcagcagcc
    18301 tttggtgact tcggatagtt tttcactacc gaagcaaaat agtacgccgc atcatctttt
    18361 ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag gtaagttgaa
    18421 tcagggtaat ttttgatgaa attctgaaat gccaccattg cgtcatcctg gcgggattta
    18481 tcctgcacca gcgcaatagc tgcattgtaa tccgtgtttg catcgccgct tttcaccggc
    18541 gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc cgcaccgctt
    18601 tgatcgctgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac
    18661 aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc ctgaatctga
    18721 ccacgcaggg aatcaatatc ggattgatta tcagaaagtt gttgctggag ttgggttaaa
    18781 agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc gaccgagcct
    18841 gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc tataccaacc
    18901 agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa
    18961 accagtaccg cacgacggtt tttggagtat gctgcttcgt catgacccag tactgcaggt
    19021 ttttctttac cgtaagaaac gatggagatc tggtctgcag aaacgccttt accctgcagg
    19081 tacatcttaa cggcgttcgc acgacgttca cccagggaga tgttgtattc cggagtacca
    19141 cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg caggaagttt
    19201 gcatgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc cagatcgaag
    19261 taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc ttcggaagac
    19321 atgttgccgt tgccgccgtt cgcatccata ccagtgccag cacccagcat gccttcgctg
    19381 ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac aggcagagca
    19441 atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt actattcaat
    19501 taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat cagttgccgg
    19561 aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc ccatcccctg
    19621 agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt catccaggaa
    19681 cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg
    19741 cccaccattg gagctgacca ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg
    19801 gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat tcactttata
    19861 aacctgcgga cgaccggcct ggtcagaagt aaatgccagg ttctggctat ccgggaacca
    19921 ggtcggttcg gtattgttac tgcgaccatc agtcacctgg cggatctgac cagaagccaa
    19981 atccattacg tacaggttca gactaccggt tttcgacaag gcgaatgcca gtttgctgcc
    20041 gtctggcgag aatgcaggtg caccgttgtg acgcgggaat gaagccacct gacgtacagc
    20101 gccatttgcc agcgtctgaa taaccagcgc ggaacgaccg ctttcgaagg tcacataagc
    20161 cagtttagaa ccgtctggtg accacgccgg agacatcagc ggctgcggtg aacggtgaac
    20221 gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga actgaccgcc
    20281 gttggtctga acaacgtagg caatacgggt acggaacgca cctttaatgc cggtcagttt
    20341 ttcaaacact tcatcactgg cggtatgacc agcataacgc agccactgct tgttcacttt
    20401 gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc
    20461 aacattgtaa gaaccatccg gattcggagt gacctgaccg acaactacag cgtcaatgcc
    20521 cagtgcggac catgcagctg gttgtacttc ctgcgcacta cccggctgct gtggcagacg
    20581 agcgcgatct aacggattaa atttaccgct gttacgcaag tcagcagcaa cgatgccgcc
    20641 aatatcttca ggtgccgcac caggccccgc ccactggaaa ggaacaacac caataggacg
    20701 accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa ctgatgccca
    20761 cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc ccttatctgg
    20821 accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca aaactaccag
    20881 aaccccgtgg caaccggtgc ctgatgttga ccgtccgaac agtcaacatc gcgattacgg
    20941 tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg gtggtttcgg
    21001 gatcttcgca agtttagctg ctgccaacgc agcctgacaa agtgcgggat cgccaccttc
    21061 aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg tacaggtttt
    21121 gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa tctgcccggc
    21181 atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac tcccggcagg
    21241 cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac cagagcttag
    21301 ctcaccgaaa atatcatctg cctctgcggc agcttttgct gcagcggctt tttcagctgc
    21361 tgcttttgcc gctgccgctt ttttgtctgc ggcggctttt tctgccgctg ctttcttatc
    21421 agctgctgcc ttatcagcag ccgctttttc tgctgctttt ttgtcggctg cagctttctc
    21481 tgctgccgct ttcttatcag ctgcagcctt atcagctgca gccttttcag cagccgcttt
    21541 cttctcagct tctgccttgg ctttttcagc agcttcagtt gccgctttct ttcttgcttc
    21601 agctgcagct gcttctgccg cttccgcttt cttcttcagt gccgcagcgg ctacctcggc
    21661 ttttttctgc gcttcggctg cggctttggc ggcttctgct tctgcttttt tcttcgcgtc
    21721 tgcagccgct ttcttcgcag cttcttccgc agctttagca tctgccgccg ctttcgctgc
    21781 cgccacttca gcttgcttct gctttaactc ggcctgtttt gcggcttctt cagcctgctt
    21841 tttctgctcc tgagccgcta accgctcttt ctcaagttgc ttcaggcgtt cctgttcagc
    21901 cgcttgtttc tcacgcagtt cttcagcagc ctgctgttcc ttcatcttgc gctgttcatc
    21961 agaacgcttc gcgcttgatt cctggctttg catgcgtttg tactgctcaa ctaccgcacc
    22021 tgaatcaacc atgacagcgt cgatggacga accaccgccg cctccggctg aagcttctat
    22081 attctcatcg aacgaactcc agatcagcgc cgcaaataag atgacatgca gcactgctga
    22141 aataattatc gcccgcttga gcttgtcgtt ttgttcggtt gcctttgaca ctctcggttt
    22201 ccaaaaactg ttcgcctgtt acccgctctc tttcaagcaa gggaaacgca gatgtttaga
    22261 taggctgcgt cattaaacca accgatttca cacccgcact atgtaacaag ttcagtgctt
    22321 taattatttc atcgtaaggc acatcttttg cgccaccgat cagaaagacc gttttcgggt
    22381 tggccttgaa acggctggac acttccgcca ccacctgctc tggtggtaaa cgctccaggc
    22441 gatctttctc aaccaccacg gtgtactgac caataccaga cacttcaaca atcactggcg
    22501 gattatcgtt actgctcacc gcctgtgatt cagtagcgtc tggcagatcg acctccacgc
    22561 tctgggtgat gatgggcgct gtcgccataa agatcagcaa cagcaccagc agtacgtcca
    22621 gcaacggtac aatgttgatt tcggacttga gatcgcgacg acctcgtcca cgcgctctgg
    22681 ccatggctta ccccttgttg ctctcgctaa cggtaaacgc ctggcggtgc agaatcgcgg
    22741 taaactcttc cataaagttg tcgtaattca gttccagttt gtttacgcgc tggttgagac
    22801 ggttataagc cattaccgcc ggaattgcgg cgaacagacc aattgccgtc gcaatcaacg
    22861 cttctgcgat accgggcgca accatttgca gtgttgcttg ttttaccgcc ccgagggcga
    22921 taaaggcgtg catgatcccc cagaccgtac caaacagacc aatatacggg ctgatggagc
    22981 caaccgtacc gaggaacgga atgtgcgttt ccagattttc aagttcacgg ttcatggaga
    23041 tacgcatagc acgcgacgcc ccttccacta cggcttccgg cgcatggcta ttggcacgat
    23101 gcaggcgcac aaactctttg aacccgctgt aaaagatttg ttccgaacca gtcagattat
    23161 cccgtttccc ctgactctct tgatagaggc gagagagttc gattccagac cagaatttat
    23221 cttcaaacgc ttcggcttca cgcgccgctg cgttaagaat acgggttcgc tggataataa
    23281 tggcccagga tgcgattgaa aaaccaatca aaatcaacat gataagttta accagaaggc
    23341 tagccttcag gaacaaatca aggatattca tgtcagtcac tgcttaaact ccgcgacaat
    23401 agacttggga agcgcacgag gcttcatttt gagtgggtca acgcaaacaa ccagaacctc
    23461 tgcttcattc agcaaagtat tctcggcgtt gacaatatgt tgcgtgaaaa ccaaagaggt
    23521 gccacgcatt gatgttattt cagtctgtat ttcgagcata tcgtcgagcc gcgcaggtgc
    23581 ataatattcc accgtcattt tacgtaccac aaaggcaacg cgttcagcca tcagcgcctg
    23641 ctgactgaag tgatgatgac gcagcatctc tgtgcgtgct ctttcataaa aagcgacgta
    23701 actggcgtgg tacaccacac caccggcatc ggtatcttca tagtagacgc gaaccggcca
    23761 tcgaaacagc gttgtattca ctttacatcc cggtaatgca acaaaagtta gagcttttaa
    23821 acgctgctac tatacgcgcg cgattgaggt ttgggaatga gggcaagtta agggagcgta
    23881 aaaatttatg ggcctagagc ggcccataat tttttaaaga gaattctgat ttaaaagaag
    23941 aagaaaatca gcccgacaat gagaacaata tcggcaagca atgggcaaaa aatcccttgc
    24001 caaagaacct tctgcggacg aaagcccacg ccgtgaatca caccagcaca gacggcccac
    24061 atcagcaata aaccatgcca gatttccagt tcactggtct tcgcggcaaa acgagatggg
    24121 tcccaaaaca tacatcctgc taacagaagc gccatcacga aggaaagcgc ccgcaggggg
    24181 cgcttgtcca ttaccgcata caaggtcgcg ataatcttac tcatcagatg tcttcttgac
    24241 cggctttgcc tgattcgacg tgttcaagcg ccagcgcggt gattacccca aacgaacagg
    24301 caagaagcgt tcccagaatc catgcgaaat accacatttt tagctcctta cttagtacag
    24361 agagtgggtg ttacgttcaa tatcttcttt ggtgatacga ccgaacattt tccagtaaca
    24421 ccaggcggtg tagagcagaa tgatcggtac cagaaccacc gcaacccagg tcatgacgtt
    24481 aagcgtcagc tggctggaag ttgcatccca cattgtcaga cttgcgttca tcatggtgct
    24541 ggacggcatc acaaacggga acattgcgat accggctgtc aggatgatgc aggccagcgt
    24601 cagggaggag aacacaaacg cccacgcggc tttatccata cgtgcagtca ggatggtcag
    24661 cagcggcaga accacaccca gtgccggaat agcccacaga attggcgtgt tgttgaagtt
    24721 aaccagccat gcgccagctt cacgaaccac ttctttattc agtgggttag aggctgcgta
    24781 atggtccatt gtcgatttca cgacataacc atcgataccg tacatcaccc atacgccagc
    24841 cagtgcgaaa cagaccagtg tcaccagcgc agccacctga gccgttgcac gggtacgcag
    24901 gtgcagttcg cccccggtac gcatttgcag ataggttgcg ccctgagtaa tgatcatccc
    24961 tacgctcacc acgcctgcca gcaggccgaa cgggttaagc aactggaaga agttaccggt
    25021 gtagtacaga cgcagatatt catcaacgtt gaacggtacg ccctgcaaca ggttaccgaa
    25081 cgctacacca attaccagcg gcggaacgaa gctaccaatg aagatgcccc agtcccacat
    25141 gttacgccag cgggtttctt caatcttgga gcggtagtca aaaccgaccg gacggaagaa
    25201 caaagacgcc agcacgagga tcatcgccac atagaagccg gagaacgcag cggcatagac
    25261 catcggccag gcagcaaaga gtgcgccgcc cgcggtgatc agccaaacct ggttaccgtc
    25321 ccagtgtggt gcaatggagt taatcataat tcgacgctcg gtgtcgttac gaccgaggaa
    25381 acgggtgagc atgcccaccc ccatgtcgaa accgtcagtg actgcaaaac caatcagcag
    25441 aacgccaacc agcagccacc agataaaacg caatacttca taatcgatca tttgacgact
    25501 cctgtcttag cgtgccggct gagtagtcgt ggaagactgc tcaaagtgat agcgaccggt
    25561 tttcaggctg cttgggccga ggcgtgcaaa cttgaacatt aagaacaatt ctgccaccag
    25621 gaacagggta tacaggccgc aaatcagcac cattgagaag atgagatcgc ctgcggtcag
    25681 tgacgaattc gccacagctg tcggcagcac ttcaccgata gcccacggtt ggcggccata
    25741 ttcagccacg aaccagcccg cttctacagc aatccacggc agcggaatac cgtacagcgc
    25801 ggcgcgcaga agccattttt tctcgccaat gcggttgcgg atgacactcc agaaagagag
    25861 cgcgatgatt gccagcagca ggaagccaca cgccaccatg atacggaacg caaagtacag
    25921 cggcgctaca cgcgggatgg agtctttggt tgcctgttga atctgcgctt cagtcgcatc
    25981 agccacgttt ggcgtatagc gtttcagcag cagaccgtaa ccgaggtctt tcttcatgct
    26041 attgaactgg tcacgaaccg cctggtcggt agaaccagaa cgcagttgtt cgagcagaga
    26101 gtacgccttc atcccgttac gaatgcgttc ttcatgctgc accatcagct ctttcaggcc
    26161 gataaccggg gtatccacgg aacgcgttgc aatgatgccc agtgcgtaag ggatctgaat
    26221 cgcaaatttg ttcgtctcct cttcctgatc aggaatgccg aacaaagtaa aggcagcagg
    26281 cgcaggttgc gtttcccact cggcttcaat agcagccagt ttggttttct gcacgtcgcc
    26341 catttcgtag ccggattcat cacccagaac aataacagac agaacagcag ccataccgaa
    26401 gctggcagcg atagcaaagg agcgtttagc gaaggcgaag tcacgacctt tcagcatata
    26461 ccatgcgctg ataccgagga tgaacatcgc gccagtcaca taaccagacg ctacagtgtg
    26521 aacgaatttc acctgagcaa ccgggttaag caccagctcg gagaagctca ccatctccat
    26581 acgcatagtt tcaaagttga aatcggacgc gattgggttt tgcatccagc cgttcgcaat
    26641 cagaatccac agtgcggaca ggtttgaacc gagcgccacc agccaggtga cacacatatg
    26701 ctgaacttta cccagacgat cccaaccgaa gaagaacaga cctacaaagg tggattcgag
    26761 gaagaaggcc atcagacctt cgattgccag cggcgcaccg aagatatccc ctacatagtg
    26821 ggaatagtaa gaccagttag tcccgaactg gaactccatg gtcagaccgg tagccacacc
    26881 cagagcgaag ttgataccaa acaacttgcc ccagaacttg gtcatatctt tataaatctg
    26941 tttgccggag aggacgtaga ccgtttccat aatggccagg aggaacgcca taccgagcgt
    27001 cagtggcaca aaaaggaagt ggtacatcgc ggtcaaggca aactgtaagc gcgacagttc
    27061 gactatatct aacatcatga ctccttgctc atcgcatgaa gactccgaga gtgaaccccg
    27121 ttagaaaggg tcacacgcat gccccaatag aaatttgttt gaacactttc atcataccat
    27181 gtgaatgcac acaaaaacgg cgtaaagaaa ggttgcaaac atgttaataa aaactcaaat
    27241 tgatcccacg tatatattac gccgcaaaat ccttacaata aacaggtttt tgttgaagca
    27301 aatttgcatt tttcgacggt gatcaattta tagctaaatt gccgcctttc agccaatttg
    27361 atcgagaaca atttatctct ttttgatgcc catttccaag acttatacat tgataaatat
    27421 caatttctac aaattatgtt aatcatgttt caacaaggta actttagtga catttatgtt
    27481 taaaatgtgt gaattataag actgaaatta gtttcggttg gcaattaaag ataaaaagag
    27541 acgaagcaaa aacagcacgg ttgaccttaa ttcttttttt ggtcactggc taatacgacg
    27601 cagagtttca ggattattaa taaaggcata aatctcctcc ccgccgatat taacatttaa
    27661 ttaacttata attatattat aattaactca cccggggtta ttattttgtt aaaaataaag
    27721 ccacatatac gtagcttgag agtgaagata aacgcaaaat tacctgatgc gctacgcttg
    27781 ccagtgtcta catcatctct gcaatatatt gaatttgcct gcttttgtag accgaataaa
    27841 gcgttcatgc cgtatccggc atgaacaaag cgccctttgt caacaatctg gccgcgcata
    27901 tgtggcttcg gtttgattgc tattcaggca agccggtaac tgaacgtccg tgactggccg
    27961 ggtaaaaaag gccccgatag atttgaacct tgattttcct cggtggtaat gtgttcatcc
    28021 atcatcgttt ctgagcaaga aatcacctcg cgactgaaag caacagtcgc atcacaggtt
    28081 gctgattcag ccggattaaa cagccgtaaa atcacttctt gtcggtcttc agctttctta
    28141 agtgcgctta tcaggcatcc cactgggggc attttcaaca aactataact ttccggcacg
    28201 ttgaatccgg ctttgttgag cttcatcgca tcccatggga ttttgttgta acactgtact
    28261 ggagtcagcc atgctcgcgc ctgctgagct acaccagcgg cggttggcgt accggtataa
    28321 ctcaataaac ttaggcgaca agaaagcaga ccacgtagtt gtgagtccgg gactggcatt
    28381 ttaattcccg aaggccgcct aggccttaaa agcagatctt ctttccccag taagcccacg
    28441 ccgcgcagca acgtaatggc aaaggttttc ttctcttcac cgatgacttc aaattcacgt
    28501 agcccttcgc taaagacagc catgccgtta cgcccttcct gtaaggctgc atagttgagc
    28561 atattccata ccggaaccgg cgcttctttc cagccttctt gctgccagtt gttcattgca
    28621 ctgtcgttca cggggcgcgt tagcgaacca aactgggtat ctgccagaac actatcggtg
    28681 ttaaaagatg tagggatcag gacgcgaagg cgatgatcgt cagcctggtt atcaaggttg
    28741 atatccacat caatgcgcct gttgttatga ctaagagtga caaccaactc gacacctact
    28801 ctgccagtgg attgtcttgc gctgcgttgt tgcagattac gcggcaccgc catttcatag
    28861 cggataacag ccctgctctg ccaggcctca tgaataatct cgcattgcgg tttcgcattc
    28921 gccgaagtaa ttacccactc ttcttttgcg ggtgaatagt catattcatc accatcatca
    28981 gagctttctt caatttgcaa cacccggtca tagcgcacac cgctatcttt atctaccagt
    29041 tgtagcgttc catcctcatt gagcgcaatt tgccagaaag cattttccag tatcccttca
    29101 gcgtcacttt ttgccgcaat cacgttgcca ggctgattcg cttcgatata aagcgtgcga
    29161 tagcccatag aagggacaat ctggttgatc tgtatatcaa actccataaa gggatcgtaa
    29221 ttaccgtaat gaactatttg ccgatcgatt aggcctggat cgatctcacg ggcatggcga
    29281 ataaaatacg gtacaggctg accgcgatcg tctcgtagat taaactggct ggcgcgcagc
    29341 cgcacagtgg tgttgatcac ttcttcccgc ggccagggca tcaggttaaa caggacgagt
    29401 ttgtcggcgt cgctctgcgg catgttgtcg gcgattttac gcatatagaa acgcagcaga
    29461 ttatccgcca tgtcttcagc cagttcgaag cgggcgacga tttcgcgatg aactttgtca
    29521 ctacagcagc agccgatgct gtcgtgggca tgatttttta agatctcttt ccatattttc
    29581 tccagcaagc cgtggtggta ttcaaaaccc aacgtccagg ccagcgttgc cagcggttcc
    29641 agcagattaa caatcttatt ttcaatacgt gcgtgggcaa ctttgatatc catgcgcgtg
    29701 gaaccgatgg tgcgatgcac gcgcatatat ttgccatcaa taaattcccc tttcagggtt
    29761 gccagattat ctcgctgcgc ttcgatcttc tcaaatacct cttcaaagcg gctcatcaca
    29821 aactttcgtt gaggataaat ttcgcgcagt ttctccatca cttcgaagat attttgctgc
    29881 aatggcatct gatcatgccc attcggcagc aaaatctctt tggttacaga cgctttttcc
    29941 agcacgtcaa aataactgtc gaggcgtttg cggaatccgt tttcgtccgc aggtaagtac
    30001 ttaccgatgg cataacccaa cggcatcacc tgcgccgtca cttcgctacc gtcactgctt
    30061 tgccacaaaa actcggtttt atccgtaccg tggcgctcag aacatccacg ccagaacatg
    30121 gtgcaggtaa tgccaaatcc attgtagata tgcggcagtt gcccggacat gccaaaggaa
    30181 tcgggtaaat aacctatttt catcggctcg ccaaacgcga ggcagtcacg cattccgtac
    30241 atcagattac ggacgatgga ttccgcagaa acaatcgtgg tatcggtctg ggtataccag
    30301 gggccgataa tcaacttgcc ggcttctacc agtttcttca cgcggtcttt gttttccggt
    30361 ttcaccgcga aataatcttc gaggatcgcc gtttgcccgt cgagtacgta atatttgtat
    30421 tcgttgtcct gttccagtcg gcacaggatc tcttccatat tattgaccag cagaatgcgt
    30481 gactcttcgg tggtgaaata ccactcccga tcccagtgca tatgcggggt gatgtgaacg
    30541 cgagataccg ctttcatcgt cgtttccttt ttgtttattg cattacgcca tcagtcagat
    30601 agttgccatg cttaaccgcg tgacgccgcc agatcaggag aattgcagtc gagattgcag
    30661 cccccaccaa tgccgcacca aaccagccaa ttgccgccat aacaccgccc gcgccattat
    30721 catgaagtaa aaagagcgag aaaatgccag caccgggtgt cgaaaggccg atattcatcg
    30781 caccgacaat agcgcccgtt accatagagc ccagcacaaa cgaaccaata acccgcagcg
    30841 gatcttcaat cgccatcggg atcgcccctt cggtaatacc tgccagccct aacagccagg
    30901 tggatttccc ggtctcaatt tcaaactctt taaacaggcg cggtgcgagc atcgtggaag
    30961 cggttacggt aaatgccgaa accattttga cggaggcgaa aatggcatac gggccgtaaa
    31021 cgccgttcgc cattgcgccc aggcagaatg cataagcggc tttattcact ggccctccaa
    31081 ggtcaaagga acacataaaa ccgagaatgg cacccagcaa cagcgcgtta cttcctgaca
    31141 gaccgttcag ccaggcggta agcgagttat tgatccaggc gacaggttcc cccaccacaa
    31201 acagcatcag actgcccgct cccaatgtac cgagcaccag gtagagataa aaagtcagga
    31261 agccattgaa tttactgcta agacgcaagt gatttttcat ccagcgtatc aagtaaccgg
    31321 ctatcaatcc gccaacgacc gcgccgagaa acccggagcc gatcatgttg gcggcaagtc
    31381 cagccgcaaa gcctggcgct aacgccggtt tatctgccag cgaataggcg gtataggctg
    31441 ccagcaccgg taccatcaaa attccgagca gcccgccgcc cagtttgcgg tacatccaca
    31501 gccaggagtt ttcttcatta aacagatctt gtagcccgaa gatttgcgac agtaataccg
    31561 cgaccgccag caccgtcccc cccgcgacaa tcaacggtac ggaaaaagag atcccgctca
    31621 acagtgcctg tttcagctcc gttttgacgc ttttcaccgg ttgcgtatct tgctgtacgg
    31681 tacgcgtctc atcgccacgc tcgagggtaa gcgattgttg gatcaacgct tctgcatggc
    31741 gaatcggctc ggcaacaggc acagaaagcg caggaatgcc gttaaaacgc tcactctcct
    31801 tgatggcgac ttcagccgca aaaatacagg cagttgcact attgagttga tccgccgtta
    31861 aacgcccttc aataccgtta gcaccttgct tttcaacgta gacattcacg ccgagtttgc
    31921 gtccggcttt ttccagatac tccgcagcca tgtaggtgtg agcaataccc gccgggcagg
    31981 ccgttacgca aacgatagtt ggcgcgttgg aaaaagaggc agaaggttgc gtgcctccct
    32041 tgtcatccag cgccgagagc aattcatcag gcgtcgtcgc cgactgtata cgcgcccgaa
    32101 tctcatcatc cgccaggcgc gtggtcagcg ctgtcagcag ttgcatgtgc gtagtacccg
    32161 cttcattggg gggaatcgcc agcaacacca ctaaatcaat cgcttccggg ccatccacgc
    32221 tttcccactg aagcggctcg ctgagtgtgg cgaccgcaaa cgccgcttct tttaccgcag
    32281 cagttttgcc atgcggcaca gccaaccctt cacctaaggc ggtcgggcca aggctttcac
    32341 ggcgatacac ttctttcaga aattgctcag tactggaaat tttccccaga gcagcaagac
    32401 gttgagtcaa cgcgtgaatg gcctcttcac ggctggtaaa gcgcgcattc agacacaacg
    32461 catcgcggtg ggtcagagtc gtcaggttca tcatgatcct cacttgatcg tttttatagt
    32521 cgcgccagtg tgcccgataa aacaatacca ttgcgtaatc aatttcactt gattgaaatt
    32581 atttgcattg tatttgtatt atttacagga tgaatttatc gggcataatg gctaaattcg
    32641 tattaatgag atgctaaata atgggacaca aacccttata ccgacagatt gctgatcgca
    32701 ttcgtgagca aattgcgcgt ggcgagctta aacctggcga tgcgttacct accgaatcgg
    32761 cattacaaac agagtttggc gtcagccggg ttacggtgcg tcaggctttg cgccagttag
    32821 tcgagcaaca gatcctcgaa agcattcagg gcagcgggac ttacgtcaaa gaagagcggg
    32881 tcaattacga tatttttcag ttaaccagtt ttgatgaaaa actgtcggac cgtcacgtcg
    32941 atacacacag tgaagttctg atatttgaag tgattccggc tgacgatttt cttcagcaac
    33001 agctacaaat caccgtgcag gatcgtgtgt ggcatgtgaa gcgcgtgcgc tatcgcaagc
    33061 aaaagccaat ggcgctggaa gaaacctgga tgccgcttgc gttgttcccg gatctcacct
    33121 ggcaggtcat ggaaaattcg aaatatcact ttattgaaga agtgaagaag atggttatcg
    33181 atcgtagcga acaggaaatc attcctctga tgccaaccga agagatgagc cgtctgctta
    33241 atatcagcca gacaaagccg attcttgaaa aagtatcgcg tggatattta gttgatggtc
    33301 gggtgtttga atatagccgc aacgctttta ataccgatga ctataaattt accctgatag
    33361 cgcagcgaaa atcatcgcga taagcacaaa aaaggccatc atacgatggc caaccatgtc
    33421 gaaaccgaca gcccttcgcc attcctgtag agggaagcgg cgagggctat ttcttattac
    33481 agatatttat ttcagaacag ttttcagtgc ttcaccgata tccgccaggc tgcgaacggt
    33541 tttcacgcct gcggcttcca gagcagcgaa tttctcatcc gcagtccctt tcccaccggc
    33601 aatgatcgca cccgcgtggc ccatacgttt gcctttcggc gcagtcacac cagcgatgta
    33661 acccacaact ggcttggtaa catgctcttt gatgtacgct gccgcttctt cttcagcgct
    33721 accgccgatc tcaccgatca tcacgatcgc ttcggtctgc ggatcttttt cgaacatttc
    33781 gagaatgtcg ataaagttag agccagggat cgggtcaccg ccgataccga cacaggtcga
    33841 ctgaccgaaa ccgtaatccg tggtctgttt aaccgcttca taggtcagtg taccggaacg
    33901 ggaaacgata cccactttac ccggtttgtg aatgtgaccc ggctggatac cgattttgca
    33961 ttcacccggg gtgataacgc ctgggcagtt cgggccgatc atacgaacgc ctgcttcgtc
    34021 cagtttcact ttcacggtca gcatatccag cgtcgggatg ccttcagtga tggtgataat
    34081 cagtttgatg cctgcatcga tagcttccag aatggagtct ttgcagaacg gtgctggtac
    34141 gtaaataaca gacgcggaag cgccagtagc agcaacagct tcacgcacgg tgttgaacac
    34201 cggcaggccg aggtgggtag tgccgccttt acctggagtt acgccaccaa ccattttagt
    34261 gccgtatgca atggcctgtt ctgagtggaa agtcccctgg ctaccggtaa agccctggca
    34321 gataaccttg gtgtttttat caattaaaat ggacattatt tcccctccac tgcggcaaca
    34381 acctgctgag ctgcatccgt cagacctttt gctgcaataa tattcaggcc gctgtcagcc
    34441 agtttcttcg cgccgagttc ggcgttgtta ccttccagac gtaccacgac cggtacgtta
    34501 acacccactt ccgctaccgc gccgatgata ccgtcagcga tcaggtcgca acgaacgata
    34561 ccgccgaaga tgttaaccag aacggctttc actttgtcgt cagagaggat gattttgaac
    34621 gcttcggtta cacgttcttt ggttgcgccg ccgccaacgt ccaggaagtt agccggttcg
    34681 ccgccgtgca gtttaactat gtccatcgta cccatcgcca gacctgcgcc gttaaccata
    34741 caaccgatgt taccgtccag cgcaacgtag ttcagttccc actgtgcagc ctgtgcttca
    34801 cgcggatctt cctgcgactg gtcacgcatt tcgcgcagat caggctggcg gaacagggcg
    34861 ttgccatcag cgcccagttt gccgtcgagg caaatcagat cgccctgttt ggtgatgacc
    34921 agcgggttga tttcgatcag cgccaggtcg cgctccagga aaatggtcgc caggcccatg
    34981 aagattttgg tgaactgctg aaccagttta ccttccagac ccagtttgaa cgccagctcg
    35041 cgtccctgat acggcatcgg gccagtcagc ggatcaagcg caactttatg gatcaggtgc
    35101 ggagtttctt ccgccacttt ttcgatttcc acgccgcctt cggtggaggc cataaagacc
    35161 acgcgacggg aactacggtc tacaacggca ccgagataca gctctttagc gatatcggtc
    35221 gccgcttcaa ccagaatctg gttaaccggt tggccattgg catctgtttg atacgttacc
    35281 agacgcttgc ccagccagtt ttctgcaaaa gcacggatgt cttctttgct gtttacaact
    35341 ttcacaccgc ccgctttacc gcggccacca gcgtgaacct gacatttcac tacccacgga
    35401 ccggcaccga tttttgaagc ggcttcttct gcttcgcgcg gagtagtaca ggcataaccc
    35461 accggtgctg gtaagccata gcgggcaaac agttgttttg cctgatattc atgtaagttc
    35521 atgtgttctg tccatccttc agtaatcgtt atcttttaaa ccgtagaccg acaccctatc
    35581 cacgtcggtc tacagtgcag gtgaagctta aactactaca cgtccagcag cagacgcgtc
    35641 ggctcttcca gcaactcttt gatcgttacc aggaagccca cggattcgcg accatcgatc
    35701 agacggtgat cgtaggacag cgccaggtac atcatcggca ggatctcaac ctgaccattc
    35761 accgccatcg gacgatcttt gatagcgtgc atacccagaa ttgcgctctg cggcgggttg
    35821 atgatcggcg tagacatcag ggaaccgaac acaccaccgt tggtgatggt gaagttacca
    35881 ccggtcagat cttcaaccgt cagcttgcca tcacggcctt tgactgccag ctctttgatt
    35941 ttcttctcga tgtctgccat gccgagggta tcgacatcac gcagaaccgg cgtcaccagg
    36001 ccgcgcggcg tagaaaccgc catgctgacg tcgaaatagt tgtggtaaac cacgtcatcg
    36061 ccgtcgatag acgcgttcac ttccgggtaa cgtttcaggg cttcaaccac cgctttcacg
    36121 tagaaggaca taaagcccag acggatgccg tggcgttttt caaacgcttc accgtactgc
    36181 ttacgcagat ccataatcgg cttcatgttg acttcgttga acgtggtcag catggcggtg
    36241 gagtttttcg cttccagcag acgctctgcc acacgcttac gcaggcgagt catcgggaca
    36301 cgtttttcac tacgtgcagc cagagccggt tgcgccgccg gagcagccgc tgccggagca
    36361 gactctttcg ccggggcttt cgccagatgt ttttccacat cttcacgagt cagacgacca
    36421 cccacaccgg tgcctttaat ggcgctggcg tcgagattgt gttcagccag caggcgacgg
    36481 atcgccgggc ttaacgcatc gttgttttgc tcttccagag acgcctgctg gcgttgcgcc
    36541 ggagtggacg ctttctcttc agatttggcg ctggtttctt taccggcgct gttgccttca
    36601 cgcaggcgac caaggatctg acgagacgtt accgttgtac cttcatcttc cagaaccgca
    36661 tccagaatgc cgtctgctga tgccggtact tccagtacca ctttgtcagt ttcgatttct
    36721 accagcactt catcacgtac gactgcgtcg ccgggttttt tatgccaggt tgcgacggtg
    36781 gcatcggcta cggattcagg caggtcaggg accagaatat ctacgctact cattgtgtat
    36841 cctttattta ttcgacgttc agcgcgtcat taaccagatc ttgttgctgt ttctggtgaa
    36901 cggacatata ccctaccgcc ggagaggcgg aggccgggcg gcctgcataa cgcagagaag
    36961 ccccaaacgg aatcacttca cggaaatgat gctggctgca gtaccatgcg ccctggttga
    37021 gcggctcttc ctggcaccag acaaaatcct tgacgtgagc aaactgctgc aacacttcct
    37081 gcatcgcttt atgcgggaac gggtagagtt gctcgatacg cacaatggcg acatcgtgtt
    37141 gattgttctt acgacgctgt tccagcaggt cgtaataaac cttaccagaa cacatcacta
    37201 cgcgcttcac gcccttcgga tcaagttcgt cgatttcacc gatggctggc aggaaggtgc
    37261 cgttcgccag ttcttcgagg ctggaaaccg ccagcggatg acgcagcagg gatttcggcg
    37321 acatcacgac cagcggacga cgcatcccgc gcagcgcctg acgacgcagc atgtggtaaa
    37381 cctgtgccgg ggtagacggt acgcaaacct gcatgttttg ctcagcacaa agttgcagat
    37441 aacgttccag acgcgcggag gagtgctccg gcccctgccc ttcgtaaccg tgcggcagca
    37501 acatcaccag accacacatc cggccccatt tctgctcgcc agaggagatg aactggtcga
    37561 taaccacctg cgcaccgttg gcgaagtcac cgaactgcgc ttcccagatg gtcagggtgc
    37621 gtggttctgc ggtggcataa ccatattcaa acgccagcac ggcttcttct gacagtacgg
    37681 agtcccagac acggaacgcg ccctgaccgt tatggatgtg ttgcagcggc gtgtaagtgg
    37741 aaccgttaga ctggttgtgg atcaccgcgt ggcggtggaa gaaggtaccg cgaccggagt
    37801 cttcacccga caggcgaacc ggaatgcctt catcaaccag cgtggcgtaa gcgaggtttt
    37861 ccgcaccgcc ccagtcgaac agtttctcac cggctgccat cgcctggcga tcgccataaa
    37921 tcttggcaac gcgagactgc atttcaactg cttctggtac cgtactgata cgtttagcca
    37981 gttcctgcag gcgcttcatc tcaactttgt tcgggtactc ttcgtcccat tcgtggttga
    38041 ggtacggcga ccaggtgaaa gagtgcatgt tcatcggacg ccactctgcc actacgcaat
    38101 cgccagcatc cagcgcatcg cggtacaggt taaccatctc ggtggcatct tccagcgtcg
    38161 ccactttttc ctgctccagc ttgtcagcgt agattttgcg cggtgtcgga tgttttttga
    38221 ttttctgata catcagtggc tgggttgcgc tcggctcgtc ggcttcgttg tggccgtgac
    38281 ggcggtagca taccaggtcg atgaagacat cacgtttaaa ggtgttacgg aaatcgagcg
    38341 ccagacgggt cacaaaggca acggcttccg gatcatccgc gttaacgtgg aaaatcggtg
    38401 cctgaaccat cttaccgata tcagtacagt acggtgtaga acgggcatcc agcgggttag
    38461 aggtggtgaa accaacctgg ttgttgataa cgatacgtac cgtaccgcca acttcataac
    38521 cacgcgcttt cgacatgttc agagtttcct gaaccacgcc ctgcccggtc actgcggcgt
    38581 caccgtggat ggtgattggc agcactttgt tgctgctcgg ctcatcaagt ctgtccagac
    38641 gggcacgaac agagccgata acgaccgggc ttacaatctc aaggtgagac gggttaaacg
    38701 ccagcgccag gtgcaccagg ccgccatcgg tctggaagtc agacgagaag cccatgtggt
    38761 atttcacgtc gcctgtgccg aggtgttctt tatgtttacc ggcgaactcg tcgaacaagt
    38821 cttgcggttt tttacccagc acgttcacca gcacgttcag acgaccacgg tgcgccatcc
    38881 cgagaaccac ttcgcgggtg ccgctgttgc cagcgtggcg gatcatctct ttaagcatcg
    38941 ggattaacgc gtcaccgcct tccagcgaga agcgttttgc gccagggaat tttgcgccga
    39001 ggtaacgctc aaggccttca gcggcggtca gttcgcttaa gaagcgtttt ttctcttcgc
    39061 tattgaaagt cgcgcgacca gactcgatac gctgttggat ccagcgtttt tcttcggtgc
    39121 tggtgatgtg catatactcg gcaccaatcg ggccgcagta ggtttgcttg agggcggaaa
    39181 taagctcgcc gagtttcatg gtttctttgc cgctggcaaa tgaaccgacg ttgaaggttt
    39241 cctggaagtc tgcttcggtc agatcgtgga aagacggatc cagatcggcc actttatctt
    39301 gctgccacag tcccagcgga tctagattcg catgctggtg accacggaag cggtatgcgt
    39361 taatgagctg caggacttta acctgcttca cattggtgtc agggtcggag atcgttgaag
    39421 agtaacgtga agcgtctttc gccaggcggc ggaaatattc acgcgtttga gagtggaatt
    39481 gatccggttt gactcccgta ccaggtaact gctggaacgt cgaacgccag ttagcgtcaa
    39541 ccgagtcagg atcggttaag aagtcttcat agagctgttc tatccagctc tggtttgcgc
    39601 cagagaggta agaagagtcc aaccaggctt tcaaagcgct gttctgcatc gtgatccctt
    39661 aagcatcttt tttatgctta cttcgccgtg gatactacca cgcacagtga tgtgcgtgcc
    39721 ggggttcact tgcgagctct tgtctacgta gtggctcgcg aaggaacctt taaaaactgt
    39781 cttagtgtca ggggcagttt ttaaagattt cctgcatttc tttgttacgc ctgatgcgct
    39841 gcgcttatca ggcctgcggg taatgacttg taggccggat aaggcgttca cgccgcatcc
    39901 ggcactggtt gcctgatgca acgcttgcgc gtcttatcag gcctacagtt tacgcattac
    39961 gttgcaacaa catcgacttg atatggccga tggcgcgcgt cgggttcagc cccttcggac
    40021 atacactgac gcagttcatg atgctgtgac agcggaatac gctgaatgca tcgctcaaac
    40081 cgtcgaggcg gctgtcagtc tcggtatcac ggctatcgat caggaaacga tatgccgcta
    40141 acaagcctgc cgggccgata aacttatcgg gattccacca gaaagacgga caagaggttg
    40201 aacaacatgc gcagagaata cattcataca gcccgtcgag tttttcgcgc tgctctggca
    40261 tctgtaaatg ctcgcgagct ggcggatttt gtccattatt caacaggtaa ggcttaattt
    40321 tctcatattg cgcatagaat tgtcccatgt ctaccaccaa atcgcggatc accggtaaac
    40381 ctggcagcgg gcgaatcaca atcttcttgc ccggctggtt gagtgccgaa atcggggtaa
    40441 tacaggccag accattcttg ccgttcatgt tcagaccgtc ggaaccgcac acaccttcac
    40501 ggcaggagcg gcggaacgac aggctgggat ctttctcttt cagctggata agcgcatcca
    40561 gcagcatcat gtcgcgacct tcttccgctt ccagggtgta atcctgcata cgcggagcat
    40621 catcaacatc cgggttatag cgataaattg aaaactcgag tctcattttc ctgtctccgc
    40681 attagtaagt acgaatcttc ggcgggaatg ccgggcgcag tttcggttcc atgttgacgc
    40741 ttcggcgcgt catggattcc gactcaggca gatacaggga gtggcacagc cagttttcat
    40801 catcacgatc cgggaagtcg aagcggctat gtgcgccacg gctttcggta cggaagttgg
    40861 cagaaacagc cgttgcatac gccgtttcca tcaggttatc cagttccagg cactcaacgc
    40921 gctgggtgtt gaactcgctg gaagtgtcat ccagacgggc atttttcaga cgctcgcgga
    40981 tcactttcaa ctgctcaagc cctttcgcca tcgcatcacc ttcacggaag accgagaagt
    41041 tatgctgcat acattcttgc agcgctttac ggatcgccac cggatcttca ccgttacgat
    41101 tattgttcca ccggttcagg cgatccagag acgcttcaac atcagactcg ctggcatcgc
    41161 gcagtgcgcc ctgctcggcg atagactctt gcagatgcag acctgccgcg cgaccaaaga
    41221 ccaccaggtc cagcagcgag ttgccgccca gacggttagc gccgtgtacc gatacacaag
    41281 cgatttcacc aacggcaaac agtcccggaa caaccacatc ttcgcctttc tcattcacag
    41341 tcagcgcctg accggtaact ttggtcggaa taccgcccat catgtagtga caggttggga
    41401 taaccggaat cggctctttc accggatcga cgtgagcgaa ggtacgggaa agctccagga
    41461 tacccggcag acgggattcg agaacttctt tacccaggtg atcgagtttc agtttcgcgt
    41521 gtggccccca cggaccatca cagccgcgac cttcacggat ttcaatcatg atggaacgcg
    41581 ccaccacatc acggcccgcc aggtctttgg cgttcggcgc ataacgctcc ataaagcgtt
    41641 cgccatgttt gttcagcaga taaccgcctt caccacggca accttcggtg accagtacgc
    41701 ccgcaccggc aatgccggtc gggtggaatt gccacatttc catatcctgc accggtacgc
    41761 ctgcgcggat agccatgccg acaccgtcgc cagtgttaat gtgggcgtta gtggtggact
    41821 gataaatacg ccctgccccg ccagtcgcca gcacggtagc gcgggctttg aaataaacca
    41881 cttcaccggt ttcgatgcac agtgcggtac aacccaccac cgcgccatcc tggtttttca
    41941 ccagatccag cgcataccac tcggagaaaa tggtggtgtg gtttttcagg ttctgctgat
    42001 aaagcgtgtg caacagtgcg tgaccggtac ggtcagcagc cgccgcagtg cgtgccgcct
    42061 gctcgccgcc gaagtttttc gactgaccgc caaacggacg ttgatagata cgaccatcat
    42121 cgagacgcga gaacggcagg cccatgtgtt cgagttccag aatcgcttcc ggcccggttt
    42181 tacacatata ttcaatcgcg tcctggtcac cgatatagtc cgaccctttt acggtgtcgt
    42241 acatatgcca ttcccagtta tcttcatggg tattacccag cgcaacggta atgccgcctt
    42301 gcgcagaaac ggtatgggaa cgggtcggga agactttaga gagcagcgca caggtctggc
    42361 cgctctggga aatttgcagc gccgcgcgca tacctgcgcc accggcacca atcacaactg
    42421 catcaaattc tctgactggc aatttcatca caccccccac accacaacga atccataaat
    42481 cacgtaaacc accagtgcaa cgacaatcac cagttgcagc atcaggcgca aggccagcgg
    42541 tttaacgtag tcggtcaaca cctgccacat gccgatccag gcatgaatca agatagaaaa
    42601 cagcgccagc agggtgaaca ctttggtgaa cgcagaggcg aagaaaccga tccagacttc
    42661 atatgtcagt tcgccactgg tggcgaaaaa accgaccata taaatgatgt agagcgtcag
    42721 gacgatagcg gtagcgcgaa cgaggatgaa atcatgtacg ccattgcgtc ctaatgcgga
    42781 ggcgttgctt accatacgag gactcctgcg agaagtgaaa gcacgacagt aataacaaag
    42841 gagattttgg cggagcgttt acccgcttcg aatgtttctt ccagatagcc aaaatccatc
    42901 atcatgtggc gaatacctac gacgacgtga tacgccagag cggtaaggat gccccacatg
    42961 ataaatttga cgaagaagct gcccataatc gcggaagctt gctcgaaacc ttcaggggaa
    43021 gagaggctgg tacccagaag ccacagcagg atgcccactg caacaaaggt gatcacaccg
    43081 gaaacgcgat ggagaatgga cgctatcgcc gtgatgggga accggatggt ctgtaggtcc
    43141 agattaacag gtctttgttt tttcacattt cttatcatga ataacgccca catgctgttc
    43201 ttattattcc ctggggacta cgggcacaga ggttaacttt ctgttacctg gagacgtcgg
    43261 gatttccttc ctccggtctg cttgcgggtc agacagcgtc ctttccataa ctgcgcgtca
    43321 tgcaaaacac tgcttccaga tgcgaaaacg acacgttaca acgctgggtg gctcgggatt
    43381 gcagggtgtt ccggagacct ggcggcagta taggctgttc acaaaatcat tacaattaac
    43441 ctacatatag tttgtcgggt tttatcctga acagtgatcc aggtcacgat aacaacattt
    43501 atttaatttt taatcatcta atttgacaat cattcaacaa agttgttaca aacattacca
    43561 ggaaaagcat ataatgcgta aaagttatga agtcggtatt tcacctaaga ttaacttatg
    43621 taacagtgtg gaagtattga ccaattcatt cgggacagtt attagtggta gacaagttta
    43681 ataattcgga ttgctaagta cttgattcgc catttattcg tcatcaatgg attctttacc
    43741 tgcaagcgcc cagagctctg tacccaggtt ttcccctctt tcacagagcg gcgagccaaa
    43801 taacaaacgg gtaaagccag gttgatgtgc gaaggcaaat ttaagttccg gcagtcttac
    43861 gcaataaggc gctaaggaga ccttaaatgg ctgatacaaa agcaaaactc accctcaacg
    43921 gggacacagc tgttgaactg gatgtgctga aaggcacgct gggtcaagat gttattgata
    43981 tccgtactct cggttcaaaa ggtgtgttca cctttgaccc aggcttcact tcaaccgcat
    44041 cctgcgaatc taaaattact tttattgatg gtgatgaagg tattttgctg caccgcggtt
    44101 tcccgatcga tcagctggcg accgattcta actacctaga agtttgttac atcctgctga
    44161 atggtgaaaa accgactcag gaacagtatg acgaatttaa aactacggtg acccgtcata
    44221 ccatgatcca cgagcagatt acccgtctgt tccacgcttt ccgtcgcgac tcacatccaa
    44281 tggcagtcat gtgtggtatt accggcgcgc tggcggcgtt ctatcacgac tcgctggatg
    44341 ttaacaatcc tcgtcatcgt gaaattgccg cgttccgcct gctgtcgaaa atgccgacca
    44401 tggccgcgat gtgttacaag tattccattg gtcagccatt tgtttatccg cgcaacgatc
    44461 tctcctatgc cggtaacttc ctgaatatga tgttctccac gccgtgcgaa ccgtatgaag
    44521 ttaatccgat tctggaacgt gctatggacc gtattctgat cctgcacgct gaccatgaac
    44581 agaacgcctc tacctccacc gtgcgtaccg ctggctcttc gggtgcgaac ccgtttgcct
    44641 gtatcgcagc aggtattgct tcactgtggg gacctgcgca cggtggtgct aacgaagcgg
    44701 cgctgaaaat gctggaagaa attagctccg ttaaacacat tccggaattt gttcgtcgtg
    44761 cgaaagataa aaatgattct ttccgcctga tgggcttcgg tcaccgcgtg tacaaaaatt
    44821 acgacccgcg cgccaccgta atgcgtgaaa cctgccatga agttctgaaa gagctgggca
    44881 ccaaagatga cctgctggaa gtggctatgg agctggaaaa catcgcgctg aacgacccgt
    44941 actttatcga gaagaaactg tacccgaacg tcgatttcta ctctggtatc atcctgaaag
    45001 cgatgggtat tccgtcttcc atgttcaccg tcattttcgc aatggcacgt accgttggct
    45061 ggatcgccca ctggagcgaa atgcacagtt acggtatgaa gattgcccgt ccgcgtcagc
    45121 tgtatacagg atatgaaaaa cgcgacttta aaagcgatat caagcgttaa tggttgattg
    45181 ctaagttgta aatattttaa cccgccgttc aaatggcggg ttaattttta tatgcctaaa
    45241 cacaaaaaat tgtaaaaata aaatccacta tcggacattc atatattcta aaaaaaaata
    45301 tatcggtata aattataaac aaatcgatga tttttaatca tctatcttcc tctcagttac
    45361 atgaataaac caccaaatat agcgcacccc tataatttcc aatactttat tcatcaaaca
    45421 tttaattcat accatcaatt aattactttt acatttaata aaattaataa tatttttaag
    45481 aaaaactaaa aacattcgac aatcaaataa gatattcttg caccgatata cttttttctt
    45541 ttctgttaaa ggactcagtt atgtttaaag gacaaaaaac attggccgca ctggccgtat
    45601 ctctgctgtt cacagcacct gtttatgcgg ctgatgaagg atccggtgaa attcacttta
    45661 aaggtgaagt tattgaagca ccgtgtgaaa tacatcagga tgatattgat aaagaggttg
    45721 aactcggtca ggtgaccacc agccacatta atcagtcaca tcacagcgat gccgttgctg
    45781 tcgacctgct cttagtcaac tgtgatctgg aaaactccag caacggttcc ggtggcaaga
    45841 tttctaaagt tgcagtcacc tttgatagct cagcgaaaac caccggcgca gatccgattc
    45901 tcaacaacac cagcacgggt gaagccacct gcgtcggcgt acgtttaatg aataaagatc
    45961 aaagcaatat cgttttaggc accgctactc cagatatcga cctggctccg acctccagcg
    46021 aacaaacgct gaatttcttt gcctggatgg aacaaattga tcaggctaca cctgtaacgc
    46081 caggcgcagt tacggcgaat gcaacctacg tgctggatta taaataagta ttactttggc
    46141 ggcgctatcg ccgcctttca taaaatattt atcaggtatg gacaccgtga atatttatcg
    46201 actctctttt gtatcctgcc tggtcgtggc gatgccttgc gcattggcgc tcgaattcaa
    46261 tctgaatgtt ctcgataaat caatgcgcga ccgcattgat atttcattat taaaggaaaa
    46321 aggagtcatt gctcccggtg agtattttgt tagcgttgcg gtaaataata accaaatcag
    46381 taacgagcaa aagattaact ggcacaaaaa tgacgataaa accattccgt gcatcaatga
    46441 tttactggtc gataaatttg gcttaaaacc tgaagtccgt cagtcattac cattgataaa
    46501 tcagtgcgtc gattttagct cccgacctga aatgctcttc aatttcgatc aagccaatca
    46561 gcaactgaat attagtattc cgcaagcctg gctggcgtgg tactcagaaa actggacccc
    46621 accctccaca tggaaagaag gtgtcgccgg tgtcctgatg gattacaact tgtttgccag
    46681 cagctaccgc ccacaggatg gcagcagcag cactaatctt aacgcctatg gaacagccgg
    46741 aatcaacacg ggggcatggc gcttacgcag tgattatcag ttgaatcata ctgatagcga
    46801 tgataaccat gaacagtcag gcgaaatatc acgtacctat ctttttcgcc cgttaccgca
    46861 attaggttca aaattaaccc tcggcgaaac ggattttagt tccaatattt tcgacggttt
    46921 ttcttatacc ggcgcggcac tggcaagtga cgatcgaatg ttgccatggg aactacgcgg
    46981 ctacgcccca caaattagcg gtattgcaca gaccaatgcc acggtgacga tcagtcaatc
    47041 aggccgcgtc atttaccaga aaaaagtccc accaggccca tttatcattg acgaccttaa
    47101 tcagtctgtt cagggcacac tggatgtcaa agtgacggaa gaagatggtc gggtgaacaa
    47161 tttccaggtt tcagcagcat cgacgccctt cctgactcgt cagggacagg ttcgctataa
    47221 actggccgcg ggtcagccac ggccctccat gtcacatcaa actgaaaatg aaaccttttt
    47281 tagcaatgaa gtttcctggg ggatgctgtc aaacacctcg ctgtacagcg gcctgctgct
    47341 ttctggtgat gactaccatt ctgccgcaat gggtattggg caaaatatgc tgtggcttgg
    47401 tgcgctgtcg tttgatgtca cgtgggccag tagccatttt gatactcagc aggacgagcg
    47461 gggcttaagc taccgtttta attacagcaa acaagtggat gccactaaca gcacgatttc
    47521 cctcgccgct tatcgtttct ccgatcgtca ttttcacagc tacgccaact atctggatca
    47581 caaatacaac gacagcgatg cgcaggacga aaaacagacg atcagcttat ctgtgggcca
    47641 accgattacc ccactaaacc tcaatcttta cgccaacctg ctacatcaaa cctggtggaa
    47701 tgcagacgcc tccacgaccg ccaacatcac ggcaggtttt aatgttgata ttggtgactg
    47761 gagagatatc tctatttcta cgtcattcaa tacaacccat tacgaagata aagatcgcga
    47821 caaccagatt tacctgtcga tttcgctccc cttcggtaac ggtggtcagg ttggctatga
    47881 catgcaaaac agtagccaca gcaccacaca ccgcatgtcg tggaacgata cgctggatga
    47941 acgtaatagc tggggcatgt ctgccggact gcaatccgac cgtcctgaca atggagccca
    48001 ggtgagcggt aactatcagc acctgagttc agcgggtgag tgggatattt ctggtaccta
    48061 tgccgccaat gattacagtt ccgtcagcag cagctggagc ggttctttca ccgcaaccca
    48121 atatggtgca gcgtttcatc gccgcagctc caccaatgaa ccacgcctga tggtcagcac
    48181 cgatggcgtg gcagatattc cggttcaggg caatctcgac tacaccaacc attttggcat
    48241 tgcggtggtg ccgttgattt ccagttacca gccttccacc gtggcggtga acatgaatga
    48301 cttacccgac ggcgtaacag ttgcagaaaa cgttatcaaa gaaacgtgga ttgaaggcgc
    48361 gataggttac aaatcactgg cttcccgttc cggtaaagac gttaacgtca tcattcgcaa
    48421 cgccagcggt cagtttcctc ccctcggagc ggatatccgc caggatgaca gcggcattag
    48481 cgtggggatg gttggcgagg aaggacatgc ctggttaagc ggagtcgctg aaaatcaaaa
    48541 gtttaccgtg gtctggggtg atagccagca ttgctcgctc catcttcctg aacatatgga
    48601 agacaccgca aatcgcctga ttttaccttg tcattaatta aggaaatgaa aatgacattt
    48661 atgaaaggac tacccctttt gttgttggtt gccagtctgt gcagccacgc tgcactacaa
    48721 cccgatcgca ctcgtattgt gtttaacgct aatgataaag ctaccagtct gcgggtggat
    48781 aatcgtagcg ataaactccc ctacctcgct tattcctggc tcgaaaatga aaaaggggag
    48841 aaaagcgatg atcttctggt cgccctgccg ccgatacaac gtctggaacc gaaagcaaca
    48901 acgcaggtac ggatagtaaa gcaagcctcg accacaaaat taccgggcga tcgcgaaacg
    48961 ctatttttct acaatatgcg tgaaattcct cccgccccgg aaaaaaacag cgaccatgcc
    49021 gttctgcaag tcgccattca gagtcgtatc aaggtgttct ggcgaccggc tgcattacgc
    49081 aagaaagcag gagaaaaggt cgaactgcag ttacaggtca gccagcaggg caaccaactg
    49141 acactgaaaa atcctaccgc gtattatctg accattgcct acctgggacg aaatgaaaaa
    49201 ggcgttctcc ccggcttcaa aaccgtgatg gttgcgcctt ttagtacggt caacacaaac
    49261 accggaaatt atagcggcag ccagttttat ctcggttata tggatgatta cggcgcgtta
    49321 cgcatgacga cgctcaactg cagcggacaa tgccatttac aagccgtgga ggcgaagaaa
    49381 tgagtgctgg caagggagtg ttgctcgtca tctgtctgtt atttctgccg ttgaagtctg
    49441 cgcttgccct gaactgctac tttggttcat ccggtggctc ggtagaaaaa tcagaagcga
    49501 ttcaaccttt tgctgtacca ggcaatgcca aactcggcga taagatctgg gaatctgacg
    49561 atattaaaat tcccgtctat tgtgacaaca ataccaacgg caactttgaa agcgaacatg
    49621 tctacgcatg ggtaaacccc tacccggggg tgcaggatcg ttattatcaa ctcggagtaa
    49681 cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac actaaccagt
    49741 gtatcgacag taagaacatt gacatttata cccttgagca gatcatcgcg atgggatggc
    49801 agaacaaaat ttgctcgggt gatcccgcta atattcacat gtcgcgtacc tttctcgctc
    49861 gcatgcggtt atacgtcaaa atacgagaaa tgccgccgca tgattatcaa agtacgctta
    49921 gcgactatat cgtcgtgcaa tttgacggtg ccggtagcgt taacgaagac cctactgccc
    49981 aaaacctgaa atatcatatt actggtctgg aaaacattcg cgtgctggat tgcagcgtca
    50041 atttttccat ttcaccggaa acacaggtga ttgattttgg taaatttaat ttgctggata
    50101 ttcgtcgcca cacaatgtcg aaaacattca gtattaagac gacaaaatca caaaatgacc
    50161 aatgcaccga cggatttaaa gtcagttcct ccttttatac cgaagaaacg ttggttgaag
    50221 aagataaagc attgttgatt ggtaatggtc tgaagctacg tttgctggat gaaaacgcct
    50281 cgccttatac cttcaataaa tatgctgaat acgccgattt caccagcgac atgttggtct
    50341 acgaaaaaac ctatacagct gaactttcgt ccattgcagg tacccccatc gaggctgggg
    50401 cccttcgata cagtggtgct ttttaaaatt aactataact gatttgccat gatgcccttt
    50461 gcagccggaa agggcatctc tttcagaaaa ttgctgattt tccccttttc cttgattgct
    50521 gttatttttt cctagcccgg ctttctttat gctgtatcga tacaccaaag agaaaataat
    50581 gagggagcga aggatgccag ttttgcagtg gggaatgtta tgtgtgctat cacttctcct
    50641 ttctattggt ttcctcgcgg tacatctccc ggcggcgcta ttacttgggc cgatgatcgc
    50701 cgggatcatc ttcagtatgc gcggaattac tctgcaactt ccccgctccg cttttctcgc
    50761 cgcgcaggcc attcttggct gcatgattgc gcaaaacctc actggttcga ttctcaccac
    50821 gctggccgcc aactggccga tcgtgcttgc tattttactg gtgacgctgc tttccagcgc
    50881 catcgtgggt tggttattgg tgcgctatag ctcactgcct ggaaataccg gtgcctgggg
    50941 ttcttctcca ggcggcgcag cggcaatggt cgctatggcg caagattacg gcgcagatat
    51001 tcgcctggtg gcgtttatgc aatatctgcg agtgctgttc gtcgcgggtg ccgctgttct
    51061 ggtcacccgc atgatgctgg gtgataacgc tgaagcggtt aaccagcaaa tcgtctggtt
    51121 cccgccagtg agcattaatc tcctgcttac gattttgctc gcagtcgttg ccggtacggc
    51181 gggatgcatg ctgcgtcttc cttccggcac gatgctcatc ccgatgctgg cgggcgcagt
    51241 gctccagtct ggtcagctca tcaccatcga actaccggaa tggctgctgg cgatggcgta
    51301 tatggcaatt ggctggcgga ttggtcttgg tttcgataaa caaatcttac tgcgggcact
    51361 acgcccgcta ccgcaaatcc tgctgtcgat ttttgctctg ctggctattt gtgcaggtat
    51421 ggcgtgggga ctgacccggt ttatgcatat tgattttatg actgcctacc tcgccaccag
    51481 tcccggcggg cttgataccg tggcggtcat cgccgcaggg agcaatgccg atatggcgct
    51541 tattatggcg atgcaaaccc tgcgcctgtt cagtattttg ctgacagggc cagccattgc
    51601 gcggtttatt tcaacctatg cgccgaagcg gtcggcctag tgctggcagc ctggacacca
    51661 gtaaaacggg cgcgaggaca acgtggtttt ctcaatgatg ccgccacagc gttcgcacag
    51721 ttcgccatca cgatgaaaaa ccttaaagcg aaacagcgcc ccatgatgct tattctcatc
    51781 cacctgcccc cgcgtagcgt aagaaaaccg aggaatctcc agtaacgcgt gtgcgagtgc
    51841 atccagttgc gccgcattga gatctttcgc tttatgattt ccagtcaacc caacctgcca
    51901 gaggatctcc acccgcaaat aattgccaag cccagccaga aacgcctgat cgagcagtaa
    51961 tcctgcaaac tgacggttac gaaagcgcgg cgacaataac cgttccttta ccacctccgg
    52021 cgtcagattc ggatccagca catcgggacc aacgcgttgt aaaaacggat gcgtggtcag
    52081 ttgttccggc ctcaacatct caatatccga ggcgctataa agcagaatgg ttttgtcagc
    52141 cgtttgcagt tttacccgca atactcgcgt ggtctgcggc tcttcgccgg tatcaaccac
    52201 gcgccagacg ccgtaaagct gattatggct gtagagcgtt aaaccgttgg gaaaatgagt
    52261 taacaacgcc ttaccacgcg tttccacatg ggtaacgtgt tgaccgataa gttgtgattg
    52321 ataagttttt aactgcggga aggcaaacca gacatcagtt agtggtttgc ctttgatcgc
    52381 cgcctccagg ttatccgctg cacggcggat ctccgggcct tcaggcatgg tgatatcctt
    52441 ttttcattct ttattaagcg gggttatgct gcgacaacaa tccccttttt ggcaaatgta
    52501 gctcgtaatc ggcgggcgaa cgccagagcg tgctcgccgt cgccatgcag gcagaccgtt
    52561 tgcgccgcga ccgttgccca ttcgccggtg atacttttga ctctgccgtg ttgcaccatc
    52621 tccagcgttt gcgccaacgc ctgttcttcg ttttcaatca acgcgcctgg ctgacttcgc
    52681 ggcaccagcg agccgtcagc ctgataaccg cgatcggcaa acacttcctc gcgcgttgtc
    52741 aggccatatt gcttgcctgc acgaatcagc tcgcttcctg ccagcccaac gagaaccaat
    52801 gccggatcgc aagcgtatac cgctctggcg atggcgtctg ccagttgtgc ttctttcgcc
    52861 gcctggttgt acaacatgcc gtgcggtttg acatgacgca ttacgccgcc ttgcgcacgg
    52921 gcaatcgttg ccagcgcgcc aatttgatac agcgtctggg cgtaaacggt ttccggcggc
    52981 agctgcatgg cgctgcgacc aaaattttcc ctgtcgggaa aactcgggtg agcgccaatc
    53041 gcgacaccat tttttattgc ttcacgcacg caagcctgca tggtttgcgc atcgcctgca
    53101 tgaaatccac aagcaatatt ggcagaggaa accagcgtta atagctctgc gtcgctggcg
    53161 cagccttcgc ccagatcggc gttcaggtca attttcattg tgcagccgcc acgctaattg
    53221 ttcaaaataa cgttgctgat cttgccgcgc tttcagtgcc tcttccagtg aacactggac
    53281 aaaatgaatc ggctgaccga gcggaatttg cgccagatgg tacatatcag cctcaatgat
    53341 acaggcaata cgtggataac caccggtggt ctgtgcgtcg ttcatcaaca caatcggctg
    53401 cccgttatgc ggcacctgca ccacgcccgg taacaaaccg tgagataaca gttcgcgatc
    53461 ggtgatgcgt tttaaaattt gcccctgtaa acgatagccc attcggttac tttgcgagct
    53521 aagctgccag ggcgaacgcc agaatgcatc ctgcgaggcg cgatcgaact catgatattc
    53581 cggccccggc aaggcgcgaa tgcggttgcc ccacagcagc tgtttaacgc cctgcgcttc
    53641 cataaaatca cgcttcgatt tgccaatcgg gagtcggtca ccatccttca gtaaacggcc
    53701 ttccagcccg ccaatcccca ctttgagatc ggtgctgcat gaccccatta ccggcggaac
    53761 atcaatacca cccgcgaccg ccagataact gcgcatcccg tgctgcgggc gtttaagcgt
    53821 taaacgctgg cctgctttca tcggcaatcg ccagccggtc cagacggcgt tatcatccaa
    53881 ccgcgcttcg caacctgcac ccgtcagggc aaaccaccca tcagtttcga actcaactgt
    53941 taactgaccg agcgtgatct ccagcgcggg ggcattggcg tcattaccca ccagcaggtt
    54001 agcaatgcgc agcgcaggca tatccagtgc gccacagtgg ctgatacccg actggcgaaa
    54061 accgtgacga ccgccatcct gcacagtggt gtacatgccc gcacgaataa tcttcagcat
    54121 attccctcct tctgcggtac aaatcgcacg ctgtctcccg gacgtaataa gatgggttcg
    54181 tcacgcgccg gatcaaacag gctgagtgag gtatgaccaa tcaactgcca gccacccggc
    54241 gttgccagcg gataaacacc agtctgcggc ccgccgatcc cgacagaacc tgccggaacg
    54301 agcaagcgcg gttcagcgcg ccgtggcgtg tgttgttgtt ccggcaaact cccgagatac
    54361 gggaagcccg gttgaaaacc taaaaaccag accacgtatt ccacggagga gtgcaattca
    54421 acaacctgtt tttcgctcaa cccgcaatac gccgcgacca ccgccaaatc cggtccgcct
    54481 gcaccaccgt aaaccaccgg aatttcaata aagcgagact caggttccag ggcctcgctc
    54541 tcctcccacc agcgttgcaa acgctctatg gcatccagcg ccagcgactc aggattacgc
    54601 aaaatcaccg tgatattgtt catgccggga atggcttcaa ccacattcgg catatccacc
    54661 agacgctgcg ccagtcgcca gatccgcttc tggctagcca gcgtcaccgg cggttccagt
    54721 tccagcacta ccgccgtttc acctatcaga taacaacgcg ctcgttgcac tttcgatccc
    54781 tctgattatt cgttatgcag gattaggaat atcaataaag gtcacatcaa gatcggtatt
    54841 ttcattcagc cactcgctca atgcgcgaat accaccgcgt tcagtggcat ggtgacctgc
    54901 agcataaaaa tgcaatccct gctcgcgggc tgaatgaatg gtctgttcag aaacttcgcc
    54961 agtaataaaa gcatccacgc caaaacgcgc ggcgctatcg ataaaacttt gcccgccgcc
    55021 cgtgcaccag gcgacgcgct ggaccacctc aggtccggta tcgccacacc ataacggctt
    55081 acgtcccaga cgcgcttcaa tccaggaggc tagttccatt cccggcacag gcatggtcag
    55141 ctctccccac ggcaccaacg gctcaatttc gcccatgacc gtgatcccca gtaacgccgc
    55201 cagctgtgcg ttattgccca gctcaggatg tgcgtcaagc ggcaaatgcc agccatacag
    55261 gttgatatca ttcgccagca acgtttttaa acggttacgc ttcattccgc gaatgaccgg
    55321 agactctcct ttccagaagt agccatgatg cacaatgacc gcatccgcgc ccagacgcac
    55381 tgcctcatcg agcaaagcct ggctggcggt gacaccggta acaatttttt gcaccgtctc
    55441 tttgccttcc acctgcaaac cattcggcgc gtaatcacta atcgccgcgc tgttcagttt
    55501 ttcgttaatc agttgttcca gttcggtgtt tttcatcttc gcgccttccg tattctgata
    55561 gctacaccgt taacatagcg ttacctcgcc tgttcgtcga tagccaatct tttgacatat
    55621 taagaaacaa acccgacgcc aaaagacgca aattgtttat atcatcacgc ttcagctaat
    55681 gtatatttat gcaatcagac agcatattgc ataaataatc agttctgtat caaagtttta
    55741 actttaagga aacacaagca cgccgcgcaa ctattacgtg acgttgccgt caattgtgga
    55801 attattatga ataaacacgc atcacaaccg cgcgctattt actatgtcgt tgcgctgcaa
    55861 atctgggaat attttagctt ttacggcatg cgtgccctgc tgattctcta tctcaccaat
    55921 caactaaaat acaacgatac tcacgcctac gagttattta gcgcctactg ttcgctggtg
    55981 tatgtcacgc caatcctcgg tggctttttg gcggataaag ttctcggcaa tcgcatggcg
    56041 gtgatgctgg gggcgttgtt gatggcgatc ggtcatgtgg tgctgggtgc cagtgagatc
    56101 catccgtcat tcctctatct gtccctggcg attatcgtct gcggctatgg cctgtttaaa
    56161 tctaacgtaa gctgtctgct cggcgagctg tatgagccaa ccgatccgcg tcgtgatggc
    56221 ggtttctcgc tgatgtatgc ggcgggtaac gtggggtcta ttatcgcacc tatcgcctgt
    56281 ggttacgccc aggaagagta cagctgggcg atgggctttg gcctggcggc ggttggcatg
    56341 atcgcgggtc tggtcatttt cttatgtggc aatcgtcatt tcactcatac ccgcggcgtt
    56401 aacaaaaaag tactgcgtgc gacaaacttt ctcctgccga actggggatg gctgctggtt
    56461 ctgctggtgg caacgcctgc actgattacc gtgctgttct ggaaagagtg gtcggtatac
    56521 gccttaattg tcgcgactat tattggcctg ggtgtactgg caaaaattta tcgcaaagca
    56581 gaaaaccaga aacagcggaa ggagctgggg ctgattgtga cgctcacctt attcagtatg
    56641 ttgttctggg ccttcgcaca acagggcggt agctcgatta gcctttatat cgaccgcttc
    56701 gttaaccgcg atatgtttgg ttataccgtt ccgaccgcga tgttccagtc gattaatgcc
    56761 ttcgcagtta tgctgtgcgg tgtgttcctg gcgtgggtgg ttaaagaaag cgtcgcaggt
    56821 aatcgtaccg tgcgcatctg gggaaaattt gctcttggcc ttggcctgat gagcgccgga
    56881 ttctgcattc tgaccttaag cgcccgctgg tccgcaatgt atggtcactc ttctctgcca
    56941 ctgatggtgt taggcctcgc ggtgatgggc tttgcggaac tgtttatcga cccggttgcc
    57001 atgtcgcaaa ttacgcgcat tgaaattccg ggcgtgaccg gcgtattaac cggcatctac
    57061 atgctgcttt ctggcgcgat cgcgaactat ctggcgggcg tgattgccga tcagacatcg
    57121 caggcttcgt ttgatgcttc cggggcgatc aactactcca tcaatgcata tattgaagta
    57181 tttgatcaaa ttacctgggg cgcactggcg tgtgtaggac tggtactgat gatttggctg
    57241 tatcaggcgc tgaaattcag aaaccgcgcg ctggcgctgg agtcataata ggccagcacc
    57301 gatgcctgat gcgacgcttg acgcgtctta tcaggcctac gtatttcctg caatttattg
    57361 aatttgcaca aatttgtagg ctggattagg cgttcacgcc gcatccggca tctgggctcg
    57421 attgcctgac gcgtctgtta tttccccttc cgcgccgtct catacgctgc caacgtttgt
    57481 actctcgcct ctttgtgctc gactatcggt tgcggataat ccagcttcac acctgctttc
    57541 tgcgcccact tccacggctc atgcaccgct ttccctggta catcgcgcag ctccggtaac
    57601 catcgacgaa taaactcgcc ctcacggtca aatttctcgc cctgagttat aggattgaaa
    57661 atacgaaaat acggcgcagc atcagtaccc gtagaagccg cccactgcca gccaccgtta
    57721 ttagctgcca aatcaccatc aatcagctgc gacataaaat atcgctcgcc ttcgcgccag
    57781 tcgatcaaca aatctttcac cagaaaactg gctgtaatca tccgtagccg gttatgcatc
    57841 cagccagtgc tgttaagctg acgcatagcg gcatcaataa tcgggtatcc cgttttgcct
    57901 ttctgccagg cctgtaaatg tgcgggattg ctttgccact gcacacgatc cgtccaggca
    57961 ataaatggac gatgtttaca caacgagggg taatacgtca tcagatgacg gtagaactcg
    58021 cgccagatca gctcactaag ccagacacta ccggccccac cgtccagcgc ctgcggctgt
    58081 tccgccaaca agcgatgcaa acactggcga ggcgataacc cgcccgttgc caagctggcg
    58141 gacaaacggc tggtgccttc cactgccgga aaatctcgtt gttgctcata ctctccagca
    58201 ccgttctcgc aaaactggcg taattgcgca atcgccgctt tttcttccac cgggaaatgc
    58261 gcagtatcga aagactgacg aggataattc agcgtaatgg atggcgcggg ctttatcgat
    58321 ccgctactac gaacttttgg tgcagcgacg cactccggca tcccttcccg cagccgtttc
    58381 agccaggcat tcttaaaagg cgtaaagact ttgtacatct cgtgattgcc ggtcatcacc
    58441 gcccccggcg gcaggatcac gctgtcatca aatccttcac acaccacgtt acgcagcgct
    58501 ctttcaacgt ccacatcccg cgctcgctca ttcacctcat actgatagtt ataaaaaaga
    58561 tgagtaacgc tgttttccgc gcacacctgt ttaactattt cgacactggc gacaaagtca
    58621 tccacttcac ggaacaataa aggaatacct ttttccgcca gcgcgatttg tagcccattc
    58681 agttgagcat tgataagttc agcctgacgc ggcgacatgt tatgcgtctc ccactggcgt
    58741 ggtgtagcga tatacagcgc cagcacgcgt gcagacgaat tgcggcaggc ggcagccagt
    58801 gcgagattat cgtgcagacg taaatcctgg cgaaaccagg ccagatgggt agtcataaaa
    58861 ctcctgaatg gcgcaagtta atcacacgcg gccaaagttc gaaataacgc tgccctgaaa
    58921 gccaggcgtc aggataaagc gccatatagt gtttgatacg catcagcggc gcaagaagtg
    58981 gttgctcgcc acggcgataa ctgtcgataa gcgcagccag ctgctggcgt tctgtggaat
    59041 caatatgcgg gcgaaaataa ccctgaacgt gcatcaaaac gttggtgtga tcgcgcgggt
    59101 tggcgggatg agaaagcaaa acaattacgc gttggcggta ctggttgtaa aaatcatcca
    59161 gattttgcca ctgatgaata tcagccacaa acgggccaag tttgcggtac tcgggctgag
    59221 aatgggccag aaagacgagt ttatagcggc tgtgataatc gagaagcgcc ccgcgcgtta
    59281 agccatgttc tttcagtcga tgtaactcat gaagggcgaa gatacggatt agggtgctgt
    59341 catcaaatcg ttgtagattc attactgctc ctgaacggta tacaggagta agtgtagacg
    59401 ttggtaaatg atgtttgtga agttaatccg ctagcagggc aagattacga aagcgcgctc
    59461 cccgcaagca actaatcaag gtagttaatc atggctgaac acgatattac gccagaagtc
    59521 accatttcca aaacacagcg ccgctataaa gtcggctatg tcagagcccg ccaccaggac
    59581 cgcaaaaccg gtatagcccg gtattatagc ctgcacctta agggcgactg gctgaaagag
    59641 gccggatttg ataccggacg gggtgtcacg gtgaagattt cggagggggg cctgaccatc
    59701 atcgctgaca gtgacgaggt gcaggaactg cgggaggaac tttatcaggt taaacaggta
    59761 gttaagggga tgcgggatgg gatgtttagt gtgctgaacg aaagttaaat attaaaaaag
    59821 ctgggaagat ttaaaacctt cctagctttt tattctgtct tattataatg tatactcatc
    59881 atcttccgat gaatttataa attgctccaa gtttacatga agagtactta acctagtttt
    59941 taactcatta agtgaagcga taatttcata ttttccaaca taatcatatg atggatcgta
    60001 ttcattatct atgtcagaag ctaaaaatga tgatattcgt agtttaaatt tagcttcatc
    60061 tttattacat tcattggcat aaggtgaatg ccctatcata gcatattcct ctaatctaaa
    60121 aggataagat ggttttaatt catcgatcaa tgaaatttca atccatgttc tatatccagg
    60181 cattaatgga ctaatttctt tgagcaaaac ctttccactt aataatgtat tgagattgtt
    60241 cattattatg tctccctcgg gaaaaatata tgtctaacac ttccaccatg ttgatgaact
    60301 tgtaaatgtg gtaaatcacc atcaggattt cctggtccat gacctgacac tctatatgga
    60361 ggttttgtta cagaaggatc ttttatatca tcccaatgat atgaacctgc ttttgtttta
    60421 tttccagtaa gatattccat taagttctta gtgctggggc ctgactcacc tgtcatattt
    60481 ttataattat gacctaaata tctatccata aacaattgtt cagcgtcact tctagaccta
    60541 acagtaactt tggtttcact actatctaat aaacgattcg ctttttggtt actaacagca
    60601 catggagtta atccaagtgg atcagcatat tttagaggat taggtgcata actgtagaga
    60661 tttaaaccgc ctctcaaccc tatcgggtca gttaccatga accgcccgac atccggatca
    60721 tagtacctgt gcagattgta gtgcagcccc gtttccctgt cgaggtactg tccctggtag
    60781 cgcaggttct gctccacccc gccgtggtcc ttctcctgta tcgtgttacc ccagacctga
    60841 taccccgtct cccacacgat tttcccgtcg ctgtccgtca tctcttccgg cgcgccgttc
    60901 acatccgtgt gaaaatacag tatccggttc gggccctctt tgcccgcctt atccacccgc
    60961 gccagcggtt cataactccc ctggtccctg tagatgtaca ggctgcctgt tccctccggc
    61021 ctgctctcct gcgccagccg cattccgtcc cataaaaatt ttgtccggtt gtacggctta
    61081 ccgtcagggc tgatgtgctg tttttccgtc cgcctcccca gtgcatcgta cacataccgg
    61141 tatgtccccc gcgctgtcgt cacttccacc atccggtgtt ccgcatcata acggtaactc
    61201 tgcgttccgc tccggccctc cttttccacc gcacggccaa attcgtcata acggtagtgc
    61261 acaccacggt aagacatcag ccggttgaac ggcaccacgc tgttttctgc cgtggccctt
    61321 tcccccgcgc gcctccccag caggtttccc gccgcgtcgt actgccagct cgccagatac
    61381 ccctcatcct ccgaacgaca cgccgtgatg ctccccgtgg ggtcgtagaa gtaatccgtt
    61441 tccccccgcc ggctgtgccg cgtcttcagc agttcatcct cgccactgta gctgtactgc
    61501 cggtccgtga acgtctccgg taccaccccg cgcatcccgc tgtatgtctc cctgcgcttc
    61561 agccgcccgc cggggtcgta aagctgccgc gtgctcagtc gcccctggct tctgagcact
    61621 tcacggtgca ggtggtcgcg cgtgaactcg ctgatgacct cccggtccag atttatctgc
    61681 tggagatggc cgctgccgta gtacagatgg cgctgcgttc tgccgtccgg aaaggtggtg
    61741 gcagtccggt tgcccagttc atcgtactgg tgggtcagga tgccctgcgc cgtctcctcg
    61801 ctcagcatcc gccccagtct gtcgtagctg aagcggataa cttccggcgc gtcagtttcg
    61861 tgccgctggc gacggatttc cagtagctgg tctgccgcat catggacgta ttccgtacgg
    61921 ccatcggctg tctcttttgc cgtgagccgg cccgccgcat cacgcgccag ccggtggcgg
    61981 atttcaccgc cccggtcccc ggtgaacacc acttccgtga ccgcgttcag cgcgttgtag
    62041 cggtacgact ggcagcagcc gtccgggttt atctgctccg ccagacggtc cagcacatca
    62101 tggcggaacc agtagcgttc tccgttctcg ttcgtgagcg tgaccagacg cccgtaagcg
    62161 tcgtacccga atgccgtcct gcgccccatc gcgtccgtcc ggctgcgcac ccggccctgc
    62221 ccgtcccgct gccagcgcgt tatcctcccc atcgggctgg tgtactttac cagcctgccc
    62281 gccgcgtctg actggtaatt caccgtgcgg ccgtccgcca gtatcacctc cgtcagatga
    62341 ccgctgtcgt catacccgta gcgcgtgctg tggctctgcg catccgtcat tctgataagc
    62401 ctcgtgcgtt cgtcgtagaa ccacgccgtc tgcgagccgg aacagtccgt gtgccgcatg
    62461 agctgcccgt cttcattcca ctggaggtat ttattccccc cgcgcgcatc tgttatctgc
    62521 accacctggc cgtggtggtc atactgatac cgggtgttct gctgcagcgg gtcggtcacg
    62581 gcaagaaggt tgccgcgttc atcgtacaca cagcgccagg tgtttcccgc cgcgtccacc
    62641 tccgtttccg gctggtgcca caccgggtgc cactgcgcct gctccacccg ccccagcggg
    62701 tcgtgcgtct ccgtgatatg cccctgcagg tcatacacat agcgccattt gccgccctga
    62761 gggtcggtca tccccagcaa cagtcgttct tcgtcgctcc agcggaatgt cgtcacctgt
    62821 ccggcttcgt cctggtacgc cgtcacctga ttctgcgcat cccactggtg ataacgtgtg
    62881 cgccccagtc cgtccgtcac gcacagcctg cgctgcgcca ggtcccacgc cagccggtac
    62941 tgttcaccgc tgtccgtgtg gtgccccgtc acccgccatg ccgcgtcatc cggaccttca
    63001 taccagcccc actggtaata atacccttcg ccgcccggct ggcggtgcag cgtcatgcag
    63061 tggttttcgt cgtaagcaaa ctcacgcagc aggtgccccg tgctgtcgta cacgccgttc
    63121 aggtggccgg cctcgtcata gctgtaacga cgcagcagta aaggtgcagt ctcagggaaa
    63181 tgctgataaa tctgtgtcac ccgccgggga tgcgccgcca gttcgtaata cagacggatg
    63241 cacggacgct gctgttcgcc gctgatttcc acaatgcggc cccggtcgtc gtagtacaga
    63301 ttcagacagt tgctgttacg gtcgcccagc attttcagcc gctggcgctg cgggtggtgc
    63361 gggtcagcct caaacagaaa acactgcccg tcatcgctgc tgataagcca gtgcccctgc
    63421 tcaccacgcc ttaccgccag cccttcaccg gggctgtaaa acccgccccc cggcagtacg
    63481 gcttccatat ccaggcgtcg ccccatgccc gacacaaagg tcatgcagtt ttcgtctgcg
    63541 ctacccggtg tgcacacgag gctgacttca tacattacac tccagccctg accaaacatt
    63601 ccttccgtac gttcgtcacg actgctgtac acgcgctgcc agtccagcgg aaagtggccc
    63661 ggcagactga aatcgaagtc atcgtcatcg gccaggtatt tggccccggt ggcggcattg
    63721 accggatgcg gaagggcact ggtggccttc tgcgtcagca tgctcatccc gacactggca
    63781 gcaaaacagg cgattttaga aagcattttg cccgggcgaa gaaacatcag cgccacggtt
    63841 gccaccaggg ttatctgcga cctgccgctt ctgatatccc tgaccaccag taacggaccg
    63901 ccaatgcgca catccggtga cacaaacgcc ccgttccccg gctcattcac caccctggcc
    63961 tcgcaggtac agcgtgcccc gctgcgtgtc gccggctggc tgttgatacg cacatcccgc
    64021 gcgccttccg cgatatattc cgctgcgggc ggctcgtcat cgcctttgaa cagattactg
    64081 atgctgccaa taaccgcatt cactccctga aaggcggttt ccagcatacc gcccggattc
    64141 agcaggttaa gggccgttcc cagggggtca tccagaaagg cggctttttt ttgtgcgaga
    64201 aactgcgtca gtgaggaagg atgtttatgg cagtcgattt tatcgtcagg ggtcggggtg
    64261 gagcccgggg ccgcactggc cacagtgggc tgccatagtt ccgacacaaa atgtgtcacg
    64321 ccatccacca tttcagaacg cccttcttcc gtactgagct ggtcgtgcag agcccgggac
    64381 atcagcacag gccctgtcac cgggttaacc gccagggcta tggcccccgc catttccaga
    64441 tattccccgg ccacatcccg gaacgttctg gcatcctcct gacgctgcgc ttcttcctgt
    64501 tctgccttcg cctgcgcttc ctgcgcctct atgtcctgcc gcgatgccgc agtcgccgct
    64561 gcccgcgcgg caggtttgct gttgatccat gtgtcaccag agccggtgag gatgtaacct
    64621 tcaatctgag gcgggaaaag catatttgcg agggctgaac aggcttcgct gaccttttct
    64681 gtcacgccgg tgtacaccat cgccacaccc gccacaaaac cggtcagcac gacggccgtg
    64741 ccccccatcg tgatccccac tgacgtcgcc agtaacgagg ttgtgccgac aatgagggca
    64801 tcaataacga catttgcagc cacctccagc accgccccca gcacatctgc cataaacgac
    64861 gtatgcagca gggcatcccc ctgtcttgcc gcccagaatg cctcttccat gtcccgttcc
    64921 ctgtatgatt aattcgtcct gacttcctgc cgcatcctga agcgcagatt ctttttaatt
    64981 tcagcccagc gcgtcatatc tgccggggtc agcggtgtgg ttctggcgta ggtgaaggcc
    65041 atcatgaccc gctcacccgg caatacaaat gccacctgac actggtacac ctgctcgtta
    65101 ccccggagaa actggctctg tatttccaaa ccgcctcgtt gagataatat tccgtcataa
    65161 tgtaaatagt ccgttttaat aaaggaatta tttattgaca ggttctgttt ttttaatcat
    65221 gaatgtaata cccatcaccg ctttacagca cactttatct ttccagccat aatcactaat
    65281 aatattaagc aattagcaat atttacaacg accagagcgg atacatagtt atgtatttaa
    65341 caatcccaat atattcacta tgcaaataaa accactagta acagacacgc taccgcctcc
    65401 atataaaaat ggacacctta taaaattgta tcttgcatag aaaaagctga aatatcggat
    65461 gcgttttaat tatcaactat aaatctataa gcttttta
//
LOCUS       DAJVQH010000009        63962 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJVQH010000009 DAJVQH010000000
VERSION     DAJVQH010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.009"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(25..1065)
                     /locus_tag="OQM87_000663"
     CDS             complement(25..1065)
                     /locus_tag="OQM87_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1506913.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLPGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(1138..1773)
                     /locus_tag="OQM87_000664"
     CDS             complement(1138..1773)
                     /locus_tag="OQM87_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS1506914.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            1901..2419
                     /gene="yhbO"
                     /locus_tag="OQM87_000665"
     CDS             1901..2419
                     /gene="yhbO"
                     /locus_tag="OQM87_000665"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCS1506915.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(2399..2842)
                     /locus_tag="OQM87_000666"
     CDS             complement(2399..2842)
                     /locus_tag="OQM87_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCS1506916.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            2893..3195
                     /gene="yhbQ"
                     /locus_tag="OQM87_000667"
     CDS             2893..3195
                     /gene="yhbQ"
                     /locus_tag="OQM87_000667"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCS1506917.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(3182..3685)
                     /locus_tag="OQM87_000668"
     CDS             complement(3182..3685)
                     /locus_tag="OQM87_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1506918.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(3679..4203)
                     /locus_tag="OQM87_000669"
     CDS             complement(3679..4203)
                     /locus_tag="OQM87_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCS1506919.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVCDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            4412..5407
                     /locus_tag="OQM87_000670"
     CDS             4412..5407
                     /locus_tag="OQM87_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1506920.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            5416..6294
                     /locus_tag="OQM87_000671"
     CDS             5416..6294
                     /locus_tag="OQM87_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1506921.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            6375..7382
                     /locus_tag="OQM87_000672"
     CDS             6375..7382
                     /locus_tag="OQM87_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCS1506922.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(7500..8744)
                     /gene="mtr"
                     /locus_tag="OQM87_000673"
     CDS             complement(7500..8744)
                     /gene="mtr"
                     /locus_tag="OQM87_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCS1506923.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(8898..10787)
                     /gene="deaD"
                     /locus_tag="OQM87_000674"
     CDS             complement(8898..10787)
                     /gene="deaD"
                     /locus_tag="OQM87_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCS1506924.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(10780..10860)
                     /gene="yrbN"
                     /locus_tag="OQM87_000675"
     CDS             complement(10780..10860)
                     /gene="yrbN"
                     /locus_tag="OQM87_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCS1506925.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(10967..11851)
                     /gene="nlpI"
                     /locus_tag="OQM87_000676"
     CDS             complement(10967..11851)
                     /gene="nlpI"
                     /locus_tag="OQM87_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCS1506926.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEHTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(11960..14095)
                     /gene="pnp"
                     /locus_tag="OQM87_000677"
     CDS             complement(11960..14095)
                     /gene="pnp"
                     /locus_tag="OQM87_000677"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCS1506927.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(14342..14611)
                     /gene="rpsO"
                     /locus_tag="OQM87_000678"
     CDS             complement(14342..14611)
                     /gene="rpsO"
                     /locus_tag="OQM87_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCS1506928.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(14760..15704)
                     /gene="truB"
                     /locus_tag="OQM87_000679"
     CDS             complement(14760..15704)
                     /gene="truB"
                     /locus_tag="OQM87_000679"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCS1506929.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(15704..16105)
                     /gene="rbfA"
                     /locus_tag="OQM87_000680"
     CDS             complement(15704..16105)
                     /gene="rbfA"
                     /locus_tag="OQM87_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCS1506930.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(16269..18917)
                     /gene="infB"
                     /locus_tag="OQM87_000681"
     CDS             complement(16269..18917)
                     /gene="infB"
                     /locus_tag="OQM87_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCS1506931.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(18942..20429)
                     /gene="nusA"
                     /locus_tag="OQM87_000682"
     CDS             complement(18942..20429)
                     /gene="nusA"
                     /locus_tag="OQM87_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCS1506932.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(20457..20909)
                     /gene="rimP"
                     /locus_tag="OQM87_000683"
     CDS             complement(20457..20909)
                     /gene="rimP"
                     /locus_tag="OQM87_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCS1506933.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(21116..21192)
                     /locus_tag="OQM87_000684"
     tRNA            complement(21116..21192)
                     /locus_tag="OQM87_000684"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21156..21158),aa:Met,seq:cat)
     gene            21540..22883
                     /gene="argG"
                     /locus_tag="OQM87_000685"
     CDS             21540..22883
                     /gene="argG"
                     /locus_tag="OQM87_000685"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCS1506934.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(22891..24516)
                     /locus_tag="OQM87_000686"
                     /pseudo
     CDS             complement(22891..24516)
                     /locus_tag="OQM87_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(24975..25061)
                     /locus_tag="OQM87_000687"
     tRNA            complement(24975..25061)
                     /locus_tag="OQM87_000687"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25025..25027),aa:Leu,seq:gag)
     gene            complement(25076..25408)
                     /gene="secG"
                     /locus_tag="OQM87_000688"
     CDS             complement(25076..25408)
                     /gene="secG"
                     /locus_tag="OQM87_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCS1506935.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(25636..26973)
                     /gene="glmM"
                     /locus_tag="OQM87_000689"
     CDS             complement(25636..26973)
                     /gene="glmM"
                     /locus_tag="OQM87_000689"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCS1506936.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRVLQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(26966..27814)
                     /gene="folP"
                     /locus_tag="OQM87_000690"
     CDS             complement(26966..27814)
                     /gene="folP"
                     /locus_tag="OQM87_000690"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCS1506937.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(27904..29838)
                     /gene="ftsH"
                     /locus_tag="OQM87_000691"
     CDS             complement(27904..29838)
                     /gene="ftsH"
                     /locus_tag="OQM87_000691"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCS1506938.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(29938..30567)
                     /gene="rlmE"
                     /locus_tag="OQM87_000692"
     CDS             complement(29938..30567)
                     /gene="rlmE"
                     /locus_tag="OQM87_000692"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCS1506939.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            30693..30986
                     /gene="yhbY"
                     /locus_tag="OQM87_000693"
     CDS             30693..30986
                     /gene="yhbY"
                     /locus_tag="OQM87_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCS1506940.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKKRKISLPR"
     gene            complement(31142..31618)
                     /gene="greA"
                     /locus_tag="OQM87_000694"
     CDS             complement(31142..31618)
                     /gene="greA"
                     /locus_tag="OQM87_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS1506941.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            31866..33299
                     /gene="dacB"
                     /locus_tag="OQM87_000695"
     CDS             31866..33299
                     /gene="dacB"
                     /locus_tag="OQM87_000695"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1506942.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(33485..34657)
                     /gene="cgtA"
                     /locus_tag="OQM87_000696"
     CDS             complement(33485..34657)
                     /gene="cgtA"
                     /locus_tag="OQM87_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCS1506943.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(34673..35638)
                     /locus_tag="OQM87_000697"
     CDS             complement(34673..35638)
                     /locus_tag="OQM87_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1506944.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(35765..36022)
                     /gene="rpmA"
                     /locus_tag="OQM87_000698"
     CDS             complement(35765..36022)
                     /gene="rpmA"
                     /locus_tag="OQM87_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCS1506945.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(36043..36354)
                     /gene="rplU"
                     /locus_tag="OQM87_000699"
     CDS             complement(36043..36354)
                     /gene="rplU"
                     /locus_tag="OQM87_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCS1506946.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            36613..37584
                     /gene="ispB"
                     /locus_tag="OQM87_000700"
     CDS             36613..37584
                     /gene="ispB"
                     /locus_tag="OQM87_000700"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCS1506947.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            37812..38090
                     /gene="sfsB"
                     /locus_tag="OQM87_000701"
     CDS             37812..38090
                     /gene="sfsB"
                     /locus_tag="OQM87_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCS1506948.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(38138..39397)
                     /gene="murA"
                     /locus_tag="OQM87_000702"
     CDS             complement(38138..39397)
                     /gene="murA"
                     /locus_tag="OQM87_000702"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCS1506949.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(39452..39706)
                     /gene="ibaG"
                     /locus_tag="OQM87_000703"
     CDS             complement(39452..39706)
                     /gene="ibaG"
                     /locus_tag="OQM87_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCS1506950.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(39866..40159)
                     /gene="mlaB"
                     /locus_tag="OQM87_000704"
     CDS             complement(39866..40159)
                     /gene="mlaB"
                     /locus_tag="OQM87_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCS1506951.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(40159..40794)
                     /gene="mlaC"
                     /locus_tag="OQM87_000705"
     CDS             complement(40159..40794)
                     /gene="mlaC"
                     /locus_tag="OQM87_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCS1506952.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(40813..41364)
                     /gene="mlaD"
                     /locus_tag="OQM87_000706"
     CDS             complement(40813..41364)
                     /gene="mlaD"
                     /locus_tag="OQM87_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCS1506953.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(41369..42151)
                     /gene="mlaE"
                     /locus_tag="OQM87_000707"
     CDS             complement(41369..42151)
                     /gene="mlaE"
                     /locus_tag="OQM87_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCS1506954.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(42159..42968)
                     /gene="mlaF"
                     /locus_tag="OQM87_000708"
     CDS             complement(42159..42968)
                     /gene="mlaF"
                     /locus_tag="OQM87_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCS1506955.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            43178..44143
                     /locus_tag="OQM87_000709"
     CDS             43178..44143
                     /locus_tag="OQM87_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCS1506956.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            44157..45143
                     /gene="kdsD"
                     /locus_tag="OQM87_000710"
     CDS             44157..45143
                     /gene="kdsD"
                     /locus_tag="OQM87_000710"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCS1506957.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            45164..45730
                     /gene="kdsC"
                     /locus_tag="OQM87_000711"
     CDS             45164..45730
                     /gene="kdsC"
                     /locus_tag="OQM87_000711"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCS1506958.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            45727..46302
                     /gene="lptC"
                     /locus_tag="OQM87_000712"
     CDS             45727..46302
                     /gene="lptC"
                     /locus_tag="OQM87_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCS1506959.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            46271..46828
                     /gene="lptA"
                     /locus_tag="OQM87_000713"
     CDS             46271..46828
                     /gene="lptA"
                     /locus_tag="OQM87_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCS1506960.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            46835..47560
                     /gene="lptB"
                     /locus_tag="OQM87_000714"
     CDS             46835..47560
                     /gene="lptB"
                     /locus_tag="OQM87_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCS1506961.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            47608..49041
                     /gene="rpoN"
                     /locus_tag="OQM87_000715"
     CDS             47608..49041
                     /gene="rpoN"
                     /locus_tag="OQM87_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCS1506962.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            49064..49351
                     /gene="hpf"
                     /locus_tag="OQM87_000716"
     CDS             49064..49351
                     /gene="hpf"
                     /locus_tag="OQM87_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCS1506963.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            49469..49960
                     /gene="ptsN"
                     /locus_tag="OQM87_000717"
     CDS             49469..49960
                     /gene="ptsN"
                     /locus_tag="OQM87_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCS1506964.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            50006..50860
                     /gene="rapZ"
                     /locus_tag="OQM87_000718"
     CDS             50006..50860
                     /gene="rapZ"
                     /locus_tag="OQM87_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCS1506965.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            50857..51129
                     /gene="npr"
                     /locus_tag="OQM87_000719"
     CDS             50857..51129
                     /gene="npr"
                     /locus_tag="OQM87_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCS1506966.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            51342..51974
                     /gene="yrbL"
                     /locus_tag="OQM87_000720"
     CDS             51342..51974
                     /gene="yrbL"
                     /locus_tag="OQM87_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCS1506967.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(51971..52699)
                     /gene="mtgA"
                     /locus_tag="OQM87_000721"
     CDS             complement(51971..52699)
                     /gene="mtgA"
                     /locus_tag="OQM87_000721"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCS1506968.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(52696..53349)
                     /gene="elbB"
                     /locus_tag="OQM87_000722"
     CDS             complement(52696..53349)
                     /gene="elbB"
                     /locus_tag="OQM87_000722"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCS1506969.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(53579..55915)
                     /gene="arcB"
                     /locus_tag="OQM87_000723"
     CDS             complement(53579..55915)
                     /gene="arcB"
                     /locus_tag="OQM87_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCS1506970.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEKSTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(56011..56940)
                     /locus_tag="OQM87_000724"
     CDS             complement(56011..56940)
                     /locus_tag="OQM87_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCS1506971.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            57615..62075
                     /gene="gltB"
                     /locus_tag="OQM87_000725"
     CDS             57615..62075
                     /gene="gltB"
                     /locus_tag="OQM87_000725"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCS1506972.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPTTTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            62088..63506
                     /gene="gltD"
                     /locus_tag="OQM87_000726"
     CDS             62088..63506
                     /gene="gltD"
                     /locus_tag="OQM87_000726"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCS1506973.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            63690..>63962
                     /locus_tag="OQM87_000727"
     CDS             63690..>63962
                     /locus_tag="OQM87_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1506974.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMR"
BASE COUNT        15629 a        17437 c        15798 g        15098 t
ORIGIN      
        1 gcatccgaca tgttttccct caaatcagaa caacagcgcc agcccgccga caatcacacc
       61 agacactgcc accatcgccc ctgtcagcca taaggctttc gccgggaacg ctttgcgcag
      121 cataatcagt gacggcaaac tcactgccgg gagcgtcatc aacaatgcca gcgccggagc
      181 ggttcccata cctgccagca tcatcgtttg tacaatcgga atttctgctg ccgtgggaat
      241 gacaaacaag catcctgcta ccgccatcgc caccacccac atcaggctgt tatcgacagc
      301 accatcggca tgggggaata accagacgcg tgcggcaccc aacaccagta ctgcaaggat
      361 gtaaaccggg atcgtactcc agaaaagtgt ccatagcgcc ctgccccagc ggctaaaaaa
      421 accgctctgt gcttccggta tgtcaatttc gaccggtgcc tgcgtttgcg gtgtttcacg
      481 cacccatttt tgcaccagcg tcgcaatcag caacaccatc accagcccgg ccaccagacg
      541 aatcgccgca aaaccccagc cgaggacaaa ccccataaac accagcgtcg ccgggtttaa
      601 caccggattg cccatccaga atgccagcgc accgcccatc gatacctgtt ggcgacgcat
      661 tcccgccgcg accggagccg cacagcaggt acacatcatg cccggcagcg aaaacagcgt
      721 tcccggcagc gtgccgcgaa agcgcgattg cccaagcgta cgcaacaacc agtcacgcgg
      781 gatcagcacc tgaatcaacg aaccgaggat cacccccagc accgccgctt tccatactgc
      841 gaggaaatag atcatcgcgt aatccaacgc cgcttgccac ggattagcat ccgcctgcgc
      901 aaggatagat ttaccgatac tgtgggtttc ggctgcagta aacgctttgc cgtagtaagg
      961 ttgccatttc acataccaga gaccggcaat gacaacgaga aagaaaagcg cgggtttcca
     1021 ccactgaatg ggcgttgccg cctgagatga agactgacca gtcatagcat tccccaggaa
     1081 taagttatga taattgagcg cgtgaatatt acgctcacta tcaattcttg ggaataatta
     1141 ttcagctctt ttgcgtaatt ctgaagagct taaaatcgtc acgccttcat gctcaggccg
     1201 catcgcctct gccagcatga cccgcgccac gtctctcgca tcaatggatt tccagttacc
     1261 tggtaacaaa cggaacagcg gcgcaaaaag cgtttcgttc atccgctgtt tgctacgatc
     1321 gcccagtaac atcgacgggc gagcaatggt caatttcggc cagttctggg caattaatgc
     1381 ttcttccatc tcccctttga cgcggttata gaaaaacggc gagtgggcat tggcacccat
     1441 agcactgacc accagcatat gctgcgcgcc cagtcgccgc ccggttaatg cggtatccac
     1501 taccagcgtg taatcggcat gaataaacgc ctctttgctc cccgcttctc gccgcgtggt
     1561 gccgagacaa caaaatacaa tgtcgatggg ttcggtgacc tgcgccaacg cgtcactcag
     1621 ttgcggatca tggggattaa acaccccggg catatcgccc aacggacgtc gcgtcggcgc
     1681 agcaatggcg ttaactttcg gttcattaat caacatccgc agcaggtgac cgcccaccag
     1741 ccccgttgcg cctgtaatca gtacctgact catcttcgct cctttacaga attgtctgcc
     1801 ttgcgctccc cggctcatgc accagtctta atagaccatg aggtaattat ccccgattgt
     1861 ggaaaattcg cctcatccaa tgcaacaacg cggaggaagc atgagtaaga aaattgccgt
     1921 tttaatcact gatgaatttg aagattcaga atttacttca cccgcagacg agttccgtaa
     1981 agccggacac gaagtgatta ctattgaaaa acaagcgggt aaaacggtga aaggcaaaaa
     2041 aggagaagcc agcgtgacca tcgataaatc catcgatgaa gtgacgccag cggagtttga
     2101 tgccctgttg ctaccgggtg gtcattcacc ggattatctg cgtggcgaca accgttttgt
     2161 cacctttacc cgtgattttg tgaatagcgg caaacctgtt ttcgccatct gtcacggtcc
     2221 gcagctgctg atcagcgccg atgtgattcg ggggcgcaaa ctgaccgcag ttaaaccgat
     2281 cattattgat gttaaaaatg cgggcgtgga attttacgat caggaagtcg tggttgataa
     2341 agatcagctg gttaccagcc ggacaccgga cgatctgcca gcgtttaacc gcgaggcgtt
     2401 acgcctgctc ggtgcctgag tcgcgtagcc aaatcatttt tttaccaaag cccagcgtgt
     2461 tgtcggtgaa tttgatttca tcgagccgga tttcccacac cggggccgac agcattctgg
     2521 caaccggaaa gcgacgattg tacgccttgc gcgcgaggtc gctttcctca ccttccagcc
     2581 tgcggatctc acctttaaac tgcacaccgc gaattaacgc taccgttttc ggctgaccgt
     2641 ttaccgttcc ggcaaccgca gcctgcggcc cactcatctg cgcgtggcgc gttttttctt
     2701 ccgtcaaaat gtagaaggcg actttctgcg catcaaaaag ataaaaggca ttagcgcacc
     2761 agagttcccc ttcctgctgc acacaccagg tgacaacatg ttgttttgcc agccaacggc
     2821 tgatggcgat gagtgtttcc attgctgttc tcttttatac tgtgggcctg accttaacat
     2881 gcgatgcaga cgatgacccc ctggtttctt tacttgatcc gtaccgccga caataagctt
     2941 tataccggga tcaccacgga tgtcgaacgc cgctatcagc agcaccaaag cggcaaaggg
     3001 gcgaaagcac tgcgcgggaa aggagaactg acgctggcgt tttctgcgcc agtgggcgac
     3061 cgttcgctgg cactacgggc ggaatatcgc gttaagcaac tgaccaaacg gcaaaaagag
     3121 cgtctggtag cggaaggcgc agggtttgca gaactgttga gcagcctgca aaccccggag
     3181 attaaaagcg attgaaatgc tcgtgatact caaccagacc ggttacgcca ttcagcgcgt
     3241 catccgctaa acgatgtacc tggaaggcgc tttcagtgcc cggccagcgg caacgcagat
     3301 cgtgatgcgc cgccagttca aagccgaaac gactgtacag cgccggatcg cccagcgtca
     3361 ccactgcggc atagccgaac tcattaagcg aatcgagtcc ttcatagacc agttggcgtg
     3421 ccagcccctg tccacggtat ttttcatcaa ccgccaacgg tgccatgccg acccattgca
     3481 ggtcttcgcc ctgcacatca accggactaa atgccacata gccaatgacc tgaccttcgt
     3541 catctgtcgc caccagcccc agcgtcagaa agccatcttc acgcagatcg tgaaccagct
     3601 tcgcttccgc atcactttcg aatgagcgac gcagcagggc atcaataccc ggcgcatcaa
     3661 tgggaatttc tactcgaatt agcatggttc acctaccgat gtctgtttgg tttcaggcgc
     3721 ggttttcatt cccgcctcaa caaaatccgc cagttgcagc agcatcatgc gtaaggcttt
     3781 cggcatttgc tccagttcaa tggcgtccat caggtttttc acatacaccc ccagctccgt
     3841 atcgccttca atcaccagcc gacgctggaa gaagagcgta tccggatctt gtttacgcgc
     3901 cgcaatcatc agcagatcgc tggcgtcggc actaaaactc acatcagctt gcgcgttctg
     3961 gctgaccacc agtttgccat tcaccaccga ggtaaaccat tgcaggtcaa tatcacacac
     4021 atgaatactt aaccagcggc cttcaagaaa ctccagctcg ccatcatcca gcgcctgacg
     4081 gaattgccag cttaagacct gctcaagaac ctggcgtttt agcgcaaatg gcgtcagttt
     4141 taccggtaca ctcaacagag atggccccaa atgcacaata cgggaacgca gtttatccaa
     4201 cacgagtttt actccctgtt tcaacaatca tcctattttg ccatatcaga aaaataacat
     4261 agcggtataa atcaacaatt ccatatgaaa ttgctgctac caccaataca actttaactg
     4321 ccttaaatca aaaattgtcg cagcaaggtt aactaaaatc ccagttcgtt aacatttttg
     4381 cgttttgata gcgcaacctt caggaaaaat tatggagctg ctctgccctg ccggaaatct
     4441 cccggcgctt aaggcggcca tcgaaaacgg cgcagatgct gtttatatcg ggctaaaaga
     4501 tgataccaat gcccgtcact tcgccggcct taactttacc gagaaaaaat tgcaggaagc
     4561 ggtgagtttt gtccatcaac atcgccgcaa acttcacatc tcgattaaca cttttgcgca
     4621 tccggacggt tacgcccgtt ggcagcgtgc cgtggatatg gcggcgcagc tgggtgccga
     4681 cgcgctgatc ctcgccgacc tcgccatgct ggagtacgcc gccgagcgtt acccgcatat
     4741 tgaacgtcat gtgtcagtgc aggcttcggc gaccaatgaa gaggcgatta acttttatca
     4801 tcgccatttt gacgttgccc gcgtggtgct gccgcgcgtg ttgtcgattc atcaggtgaa
     4861 acagctggca cgggttactc ctgtcccgct ggaagtgttt gctttcggca gcctgtgcat
     4921 tatgtcggaa ggccgttgct atctgtcgtc gtatctgacg ggtgagtcgc ccaacactgt
     4981 gggtgcgtgt tctccggccc gtttcgtgcg ctggcaacaa acgccgcagg ggctggaatc
     5041 ccgcctgaac gaagtgctga tcgaccgtta tcaggacggc gaaaacgcag gttatccgac
     5101 gctgtgtaaa gggcgttatc tggtggacgg cgagcgctat cacgcgctgg aagaaccaac
     5161 cagtctcaat accctggaac tgctgccgga gttaatggct gcgaatattg cttcggtgaa
     5221 aattgaaggc cgccagcgta gcccggcgta tgtcagccag gtggcgaaag tctggcgtca
     5281 ggctatcgac cgttgtaagg ccgatccgca aaacttcgta ccgcaaagcg cgtggatgga
     5341 gacgctcggg tcgatgtccg aaggcacgca aaccaccctt ggcgcgtatc accgtaaatg
     5401 gcagtgagaa gagcaatgaa atattcctta gggccagtgc tgtggtactg gccaaaagag
     5461 acgctggaag atttttatca gcaagccgcc gccagcagcg ccgacgtgat ttatcttggt
     5521 gaagcggtat gcagcaagcg ccgggcaacc aaagttggcg actgtctgga gatggcaaaa
     5581 tcgctcgccg gaagtggcaa gcagattgtg ctctccacgc tggcgctggt gcaggcatcc
     5641 tctgaactgg gcgaactgaa acgctatgtt gagaacggtg agtttctgat tgaagccagc
     5701 gatctcggcg tggtgaatat gtgcgctgaa cgcaaactgc cgttcgtcgc cgggcacgcg
     5761 ctgaactgct acaacgcggt gacgctgaaa atattgctca aacagggcat gatgcgctgg
     5821 tgtatgccgg tggagctttc ccgcgactgg ctggtgaatc tgcttaatca gtgcgatgag
     5881 ctgggcattc gcaaccagtt tgaagtggaa gtcctgagct acggtcatct accgctggcc
     5941 tactccgccc gctgctttac cgcgcgttcg gaagaccgcc cgaaagatga gtgtgaaacc
     6001 tgctgcatta agtatccgaa cgggcgcaac gtgctgtcgc aggaaaacca acaagtgttt
     6061 gtactcaatg gcattcagac catgagcggc tacgtttaca acctcggtaa cgagctggca
     6121 tccatgcagg gattggttga tgtagtccgc ctgtcaccgc agggcactga cactttcgcg
     6181 atgctcgacg ccttccgcgc taatgaaaat ggcgctgtgc cactgccgtt gacggcaaac
     6241 agcgactgta acggctactg gcggcggctg gcaggactgg agctgcaagc ctaagtaaat
     6301 agctcacttt gttaacaact ttaactattc tttaatgcag tattaaagat taatcggtaa
     6361 caaagtgagc tgttatgact gataaaacca ttgcgttttc gctactcgat ctggccccca
     6421 tacccgaagg ctcttcagcg cgggaagcgt tctcccactc tctcgatctc gcccgtctgg
     6481 ctgaaaagcg cggctatcat cgctactggc tggcagaaca ccacaatatg accggcattg
     6541 ccagtgctgc aacatcggta ttgattggct atctggcggc gaataccacc acgctgcatc
     6601 tggggtctgg cggcgtgatg ttgcctaacc actcaccgtt ggtcattgcc gaacagttcg
     6661 gcacactcaa tacgctctat ccggggcgaa tcgatttggg gctgggtcgt gctccgggta
     6721 gtgaccaacg gacaatgatg gcgctacgtc gtcatatgag cggcgatatt gataatttcc
     6781 cccgcgatgt cgcggagctg gtggactggt ttgacgcccg cgatcccaat ccgcatgtgc
     6841 gcccggtacc aggctatggc gagaaaatcc ccgtgtggtt gttaggctcc agcctttaca
     6901 gtgcacaact ggcggcgcag cttggtctgc catttgcgtt tgcctcacac ttcgcgccgg
     6961 atatgttgtt ccaggcgctg catctttatc gcagtaactt caaaccgtcg gctcggctgg
     7021 aaaaaccata cgcgatggtg tgcatcaata ttatcgccgc cgacagcaac cgcgacgctg
     7081 aatttctgtt tacctcaatg cagcaagcct ttgtgaagct gcgccgtggc gaaaccgggc
     7141 aactgccgcc gccgattcaa aatatggatc agttctggtc accgtctgag cagtatgacg
     7201 tgcagcaggc gctgagtatg tcgttggtag gcgataaagc gaaagtgcgt catggtttgc
     7261 agtcgatcct gcgcgaaacc gacgctgatg agattatggt taacgggcag attttcgacc
     7321 accaggcgcg cctgcattcg tttgagctgg cgatggatgt taaggaagag ttgttgggat
     7381 agtgtgtctt aacgcgggaa gccttatccg agctggcaac gctgtcctac gtaggcctga
     7441 taagcgaagc gcatcaggca ttgtgtaggc agcagaaatg tcggataagg caccgctgat
     7501 tactgataca ccggcagtaa attaaagctc gataaaatat gcaccagtgc gttgccgacg
     7561 ccaaacacca gaatcagcgc aatcatcggc ttgccacccc agacgcggaa tttcgggctg
     7621 ccaaagcgtt tacgcgatgc gcgggccagc agtgccggaa caattgccgc ccagatggta
     7681 gccgctaaac cagcataacc aatggcatac aggaatccgt tcgggaacag cagaccaccc
     7741 acaacaggcg gggcaaaggt cagcaatgcc gttttcaagc ggcccacagc cgagtcgtcg
     7801 aaaccaaaca gatctgccag atagtcaaac aaacccagcg ttacgccaag gaacgaactc
     7861 gctaccgcaa agtttgagaa cacgaccagc agaaggtcca ggctacggct gttcagcacg
     7921 ccgcttaatg cttgtaccag cacatcaata ttaccgccct tctcggcgat gccgataaac
     7981 tccggacgag ggatgttgcc cattgtcgcc agcaaccaga tggtatacag cgccagtgcc
     8041 atcagcgtac cgtacaccag gcatttcacg atggttttcg gatctttgcc gtaatacttc
     8101 atcaggcttg gcacgttacc gtgataacca aacgacgcca agcagaacgg cagggtcatc
     8161 agcaaatacg gcgcataaga tgcattgctt tcggcaacgt tgaacaatgt cgcaggctgc
     8221 acatgcccca gcaggctacc gaaggtgagg aagaaggtaa tgactttcgc ccccagcaca
     8281 atcgccgtca tgcggctgac ggctttagta ctcaaccaca ccacaaacgc taccagcagt
     8341 gcaaaaccaa aacccgccgc ccgtgccggg acgtttagcg acatctctgc gaaggtgtga
     8401 tgcagaatcg aaccactggc agaaatatag gcataggtca ggatatagag cacaaaggca
     8461 atggaaatgc cgttgaccac gttccagcct ttgcccagca aatctttggt gatggtgtca
     8521 aaactcgaac cgattcggta attcaggtta gcttccagaa tcatcaagcc ggaatgcagc
     8581 atacagaacc aggtaaagat cagcgctgcc attgaccaga aaaaccacgc cccggacatg
     8641 accactggca gagaaaacat ccctgcgcca ataatggtgc cgccgataat caccacgccg
     8701 ccaagcagcg acggtgacgt ttgggtggtg gttagtgttg ccatgagggc ttctctccag
     8761 tgaaaaatag tgcgactgcg ttgttatgca ttgcactgta ccagtacatg agtacaaaag
     8821 acagaaaaaa agccccgatg gttaaaatcg gggctgtata tattatttta cagattgtgt
     8881 tcgctgttca gcgatgatta cgcatcacca ccgaaacgac gacgaccggt agagtcatca
     8941 cgacgcggtg cgcggccttc acgacgttcg ccgctaaaac gacgaccatc accacgacca
     9001 ccttcacggc gttcaccgct gaagttacga ccgccttcac gacgctcgcc accgaaacca
     9061 cgaccaccgc cacgaagctc accgccagta tgcggctgtg catcgcccag taactgcatg
     9121 ttcatcggct tgttgagaat gcgagtgcgc gtaaagtgtt gcagcacttc acccggcata
     9181 cctttcggca gttcgatggt ggagtgagaa gcaaacagct tgatgttacc gatgtaacgg
     9241 ctgctgatgt cgccttcgtt agcaatcgcg ccaacgatat gacgaacttc aacaccatca
     9301 tcgcggccca cttcaatgcg gtacagctgc atatcgccaa catcacgacg ttcacgacgc
     9361 ggacgatctt cacggtcgcc acgcgggcca cggtcgttac gatcgcgcgg accacggtca
     9421 tcacggtcac ggaattcacg tttcggacgc atcggcgcat ctggcggaac aatcagagta
     9481 cgttcaccct gtgccatttt cagcagtgcc gcagccagag tttcgagatc cagctcttca
     9541 ccttctgcag tcggctgaat tttgctcagc agtgcgcggt attgatccag atcgctgctt
     9601 tccagctgct gctgtacttt agcggcgaat ttttccagac ggcgtttgcc cagcagttct
     9661 gcgttcggca gttctacttc cggaatagtc agcttcatag tacgttcaat gttgcgcagc
     9721 agacgacgct cgcggttctc aacgaacagc agcgcgcggc cagcacgacc cgcacgaccg
     9781 gtacgaccga tacggtgaac gtaagactca gaatccatcg ggatatcgta gttaaccacc
     9841 aggctgatac gctcaacgtc caggccacgg gccgcaacgt cggtcgcaat caggatgtcc
     9901 agacgaccgt ctttaaggcg ttccagtgtc tgctcacgca gcgcctggtt catgtcaccg
     9961 ttcagcgcgg cgctattgta gccgttacgc tcaagagctt cagccacttc cagagtcgcg
    10021 tttttggtac gaacgaagat aatcgccgca tcaaaatctt ccgcttccag gaaacgtacc
    10081 agtgcttcgt ttttacgcat accccacaca gtccagtagc tctggctgat gtcaggacgg
    10141 gtagtcacgc tggactgaat acgcacttcc tgcggctctt tcataaagcg gcgggtaatg
    10201 cgacgaatcg cttccggcat ggttgcagag aacagagcgg tctgatgacc ttccgggatc
    10261 tgcgccataa tggtttcaac gtcttcgatg aagcccatgc gcagcatttc gtcagcttca
    10321 tccagaacca gaccgctcag tttagagagg tccagagtgc cacgtttcag gtggtccagc
    10381 agacggcccg gagtaccgac aacgatctgc ggcccctgac gcagggcgcg taattgcacg
    10441 tcataacgct ggccgccgta cagggcaacc acatttacgc cgcgcatgtg tttagagaaa
    10501 tccgtcattg cttcagcaac ctgtaccgcc agttcgcggg tcggtgccag caccagaatc
    10561 tgtggtgctt tcagctcagg atcaagattc tgcagcagag gtaaagagaa tgctgcagtt
    10621 tttccgctcc ccgtctgggc catacccaga acgtcgcggc cattcagcag atgtggaata
    10681 cactctgcct gaattggaga tggtttttcg taacccagat cgttaagggc ttcaaggata
    10741 ggagccttca ggcccagatc tgcaaaagtg gtttcgaatt cagccatgta gtacgtgtgc
    10801 ctcaaaatta atggcggcca gtctacataa ctcatcatga aattgatcag caattttcat
    10861 tgaaaagtgt gaaccggctc aaagtaggtg tattaacgaa caacaacgcc ctcacccgtt
    10921 aaggtgatgg caatcaaaaa agattacggg ctgatgtgta cgtcagctat tgctggtccg
    10981 attctgccag gtcatcttgg tcctggccca ggagcgataa ttccaacaat gcgtatcggt
    11041 gctcaacaaa gttatgaacg ttgttggcaa ccgccagttt gaacagtgcc gtggcgctgt
    11101 ccaaatcccc cagacttagg tagtacttac ctaaatagaa gttggtttca ctgagatgct
    11161 cagcgagcga ggtgttatcg gttgcgtccg ccttgagcct ttccattaac gtgtgttcgc
    11221 taatgttgcc caggtagaac tcgacaatgt tccatcccca ctgttcctta tccgattttt
    11281 cgaagtgctg tttcaacact tctttagcct gcttctcatc gagcttctgc tcggcgagat
    11341 aaagccacag actacggaaa ggatcattgg gatcgtcttg ataaaacgcc agcagatcat
    11401 cttgcgctaa cttgtcacga ccgccgtaat ataatgcaat cccgcgattc aagtgcgcgt
    11461 agttgtaagt tggatcaagc tcaagtacag aatcaaacgc ttcataggca gcatcaaaat
    11521 tgcctgcctg cgttaaatat atgcctaagt aattgaatac ttcaggcata tccggtcgga
    11581 ttgccagcgc ttgcgaaaaa tcgttacgcg ctaatgccct cagaccgaga ctatcataca
    11641 acactccgcg ctcatataaa agctgtgcgc gttcgtcatc ggttaaagcc cgactggcaa
    11701 ggatttgttc catacgtgcc agaatcactt cctgctgtaa agtcggttgc aatggtaccg
    11761 cgaggacttc acttttacgc caggaagtat tactgcatcc tgcaagcgta agtgctgtcg
    11821 caacgaaaca ccagcgcaaa aaaggcttca tttcccactc ccgaagacca cggttgaatg
    11881 aacgtcctgt tcccggttgc taacaaggcg tcctgcccgg ttaaaagccc cccgccgcag
    11941 cggagggcaa atggcaacct tactcgccct gttcagcagc cggagcttcc ggtgctgcag
    12001 caggttgaga ctgctcagtc gcttctttaa tgctcagacg gatacggccc tggcgatcaa
    12061 cttccagaac tttcaccggt acttcctgac ccatctgcag gtaatcggtc actttctcaa
    12121 cgcgtttgtc agcgatttga gagatgtgta ccagaccttc tttaccgccg ccgatggcaa
    12181 caaatgcgcc aaagtcaacg atacgggtca ctttaccagt gtagacgcgg cccacttcga
    12241 tttctgcagt gatctcttcg atacgacgaa tagcatgttt cgctttctcg ccgtcggtcg
    12301 ctgcaatctt cacagtaccg tcatcttcga tttcgatggt ggtgccagtt tcttcggtca
    12361 gggcacggat aacagagccg cctttaccga taacgtcttt gatcttgtcc gggttgatct
    12421 tgatggtatg gatacgcggt gcgaactcag agatatcgcc acgcggcgcg ttgatcgcct
    12481 gttccattac gcccaggata tgcagacgcg cacctttagc ctggttcagc gcaacctgca
    12541 tgatctcttt ggtgatacct tcaattttga tatccatctg caatgcagag ataccctcgc
    12601 gggaacccgc aactttgaag tccatatcgc ccaggtgatc ttcgtcgccc aaaatgtcag
    12661 acagtacaac gtagttgtcg ccttctttca ccagacccat tgcgataccc gcaacggcag
    12721 ctttgatcgg cacacctgcg tccatcagcg ccagagacgc gccgcacacg gaagccatag
    12781 aagaggaacc gttggattca gtgatttcag acacaacacg tacggtgtac gggaatttgt
    12841 ccatatccgg catgactgcc agcacgccgc gcttcgccag acgaccgtga ccaatttcac
    12901 gacgcttcgg agaaccgacc atgccggttt cgcctacgga gtacggaggg aagttgtagt
    12961 ggaacaggaa ggtatcggta cgttcgccca tcagttcatc aagaacctgc gcgtcacgag
    13021 cagtacccag cgttgcggta accagcgcct gcgtttcacc acgggtgaac agcgcagaac
    13081 cgtgagtacg cggcagcacg ccagtacgca catccagacc acggatcata tctttttcac
    13141 gaccgtcgat acgcggttcg cctgccagta cgcggctacg aacaacgttt ttctcgatcg
    13201 cgtgcagaat ttcacccagt tcgttttcgt ccagggtttc gtcttcagca agcagcgtcg
    13261 cgatggtttc agatttgatg acatcaacct gcgcataacg ctcttgtttg tcggtgatgc
    13321 ggtaagcatc gctcaggcga gcttcagcca gtgcagcaac gcgcgcgttc agcgcttcgt
    13381 ttaccggctc cggctgccag tcccaacgtg gtttaccggc ttctttcacc agttcattga
    13441 tgttctgaat aacaacctgc tgttgttcat gaccgaacac tactgcgccc agcatctggt
    13501 cttcgctcag cagttcagct tcagattcaa ccatcagtac agcggcttca gtaccggcaa
    13561 caaccagatc cagtttgctc tctttcaact cgtcctgagt cgggttcagt acgtactggt
    13621 cattgatgta acctacgcga gcagcaccaa tcggaccatt gaacggaata ccagacagag
    13681 acagtgctgc ggaagcacca atcatcgcga cgatatccgg gttaacttgc gggttaacag
    13741 aaaccacggt ggcgataacc tgaacttcgt tgacgaagcc ttccgggaac agcgggcgaa
    13801 tcgggcggtc aatcagacgc gcgatcaggg tttcgccttc gcttgggcgg ccttcacgac
    13861 ggaagaagct acccgggata cgaccagcag cgtaggtacg ctcctgatag ttaacggtca
    13921 gtgggaagaa gtcctgacct ggtttggctt ttttctggcc aacaacggta acaaataccg
    13981 cggtgtcatc catgctaacc ataacagcgg cagtagcctg acgagccatc atgccggttt
    14041 ccagagtcac ggtgtgttgg ccgtactgga atttacgaac gatcggatta agcaatgtaa
    14101 tatcctttct ctttcttaga cagtacctta cggcactggt gttaataccc gatcttctgc
    14161 gcatcctcgc gactaatgac aaccctaacc cagctctatg tgggtaaagc ctctcattag
    14221 ccgcgcgaac ctctgcaacg gaagatcatt catagcaaca atacattagt ttccagtgaa
    14281 ttgctgccgt cagcttgaaa aaaggggcca ctcaggcccc tttttctgaa actcgcaaga
    14341 attagcgacg cagacccagg cgctcgatga gctgggtgta acgtgctacg tctttacgtt
    14401 tcaggtagtc gagcagttta cgacgctgag aaaccatgcg cagcagacca cgacggctgt
    14461 ggtgatcttt tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt gcagtcagca
    14521 gtgctacctg aacttcagta gaaccggtgt cgtttgcgtc acgaccaaac tcagaaacga
    14581 ttttagctgt tgcttcagta cttagagaca ttttaaaact ccaaagtata tagaatgaaa
    14641 ggacgccgat ctctaattca gcgatcccag tgtacgttac gcaaagtgtt aaacaattta
    14701 cgcgacgtta agcggcagta ttctactcgt agcgacctgt tatcgcaaga cggttaacat
    14761 tacgccgggt attcaaccac caggcgacga ggcgcaacgc ggccttcatc gtcaatttca
    14821 cccataccga taaatttgcc gttctcacct tctgtaacgc gaaccagtcc ttccagcggc
    14881 gcaccggatg tacgaactgg gttaccattt ttgaagtaaa cagaagacgt taacggaaga
    14941 ttcaccaccg ggtagtccga agctggactg tccattggca tcagtaatgg atcaagtaac
    15001 tccgcagccg gaatatcctg ctgttcggct tgttcgacca gttcacgcag atgttccagg
    15061 gtcacaatcc gttcaaccgg atatttactt accgccagac gacgcaggta aataacatgc
    15121 gcgccacaac cgagtttttc acccaggtca tcaatgatgg tgcggatata agtgcctttt
    15181 gagcagtgaa tttccagctc cagctcattg ccttcgtggc gaataaacag caattcataa
    15241 acggtaatcg ggcgcgcttc acgcggtact tcaatgccct gacgcgcata ttcgtacagt
    15301 tttttaccct gatatttcag tgctgaatac atcgaaggga tctgttcgat atcgccacgg
    15361 aaagtatcca gcgccgccgc cagttgctct gcgctaaagg ttaccggacg ttcttcaacg
    15421 atttgtccgt cggcatcaga agtatcggta cgctgtccaa gacgcgcaat gacccgatag
    15481 cgtttgtcgg agtccagcag atactgggaa aacttcgtcg cttccccgag gcaaattggc
    15541 aacatgccag ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt ggcgttataa
    15601 atccgtttca ctttttgcag cgcatcattg ctggacatac cctgcggttt atccagcaac
    15661 aaaacgccgt taatgtcgcg accgcgacga cgaggacgac tcattagtcc tccttgctgt
    15721 cgtccgggtt aacacgacgt tcttcgtcat gtttgaccac gctggtcacc aggtttgaca
    15781 tgcgcatccc ttcaaccaga gagttgtcgt agaagaaggt cagttccggc acgatacgca
    15841 ggcgcatcgc tttccccagc aggctgcgga tgaaaccaga agcttcttgc aacgctttga
    15901 tgcccgcttt aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat actttggcat
    15961 acgccaggtc gcgagacatt tcgacaccgg aaacggtggt catcatgccc aggcgaggat
    16021 ctttaatttc acgctgcagg atgagagcga tctctttttg catttcctgc gctacgcgct
    16081 gcgggcgacc aaattctttc gccataataa attctcctga caaaaaaggg gctgttagcc
    16141 cctttttaaa attaatttca ggtggaaggg ctgttcacgt tggcctgata agacgcgcca
    16201 gcgtcacatc aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg catggaaccc
    16261 taaaaacctt aagcgatggt acgttggatc tcgatgattt cgaatacttc gatcacatcg
    16321 ccagtgcgga cgtcgttgta gttcttaacg ccgataccac attccatacc gttacggact
    16381 tcgttaacgt catctttgaa gcggcgcagg gactccagct cgccttcgta gataaccacg
    16441 ttgtcacgca gaacgcggat cgggttgtga cgtttaacca caccttcggt aaccatacag
    16501 cctgcgatgg caccaaattt cggcgatttg aacacgtcac gaacttccgc cagaccgata
    16561 atctgctgtt tcagttccgg agacagcata ccgctcatcg ccgctttcac ttcgtcaatc
    16621 aggttataga tgacggagta gtaacgcaga tccaggcttt ccgcttcaat cactttacgt
    16681 gcagaggcat cagcacgtac gttgaagcca accaggatgg cgttggacgc cgcagccagg
    16741 gtggcgtcgg tttcggtgat accacctacg ccagaaccga tgatcttcac tttaacttcg
    16801 tcagtagaca gtttcagcaa ggagtcggag atcgcttcca cagaaccctg tacgtctgcc
    16861 ttcaggacga tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa catgttctcg
    16921 agtttagatt tctgctgacg cgccagttta acttcgcgga atttaccctg acgatacagt
    16981 gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc cgcagccggt
    17041 acgccggaca ggccgaggat ttccaccgga atggacggac ccgcttccag cacttcctga
    17101 cccagttcgt tacgcatcgc acgaacacga ccgtattcga agccacacag aacgatatcg
    17161 cccttgtgca gagtaccttc acgtaccaga acggtagcaa ccggaccacg acctttatcg
    17221 aggaaggatt cgataaccgc accgctcgcc atacctttac gtaccgcttt cagctccaga
    17281 acttccgcct gcagcaggat agcgtccagc agttcatcga taccagtacc cgctttcgca
    17341 gacacatgta cgaactggct ttcaccgccc cactcttccg gcaggatgcc gtactgggag
    17401 agttcgttct taacgcgatc cggatcggct tctggtttat cgatcttgtt cactgcaacg
    17461 accaccggaa cctgcgccgc tttcgcgtgc tggattgctt cgatcgtctg cggcattacg
    17521 ccgtcgtcgg cagcaacaac caggactacg atgtccgttg cctgcgcacc acgagcacgc
    17581 attgaagtaa acgcggcgtg ccccggggtg tccaggaagg tgatcatgcc gttttcagtt
    17641 tcaacgtggt atgcaccaat gtgctgagta atgccgcccg cttcgccaga ggccactttc
    17701 gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt caacgtgacc catgatggtc
    17761 acaaccggcg cgcgcggttc agccgcagca cccgtgtcac ggtcgctcat taccgcctct
    17821 tccagctcgt tttcacgacg caggataact ttatggccca tctcttcagc aaccagctgt
    17881 gcggtttcct gatcgataac ctggttgatg gttgccattg cgcccagttt catcatcgct
    17941 ttgatgacct gagagccttt aaccgccatc ttgttcgcca gttcgccaac ggtgatagtt
    18001 tcgccgatca caacgtcacg gttaacggcc tgagcaggct tctggaagcc ttgctgcagc
    18061 gaagaacctt tacgttttcc gcctttaccg ccacgtactg ctgcgcgtgc ttcttcacga
    18121 tcagcttttg attcagcgtg tttgttgcct ttcttcggac gcgctgcttt cgcgttacga
    18181 ccacggccac ggccgccttc gacttcacga tcgctttcgt cttctgcctg gcgagcatgt
    18241 tgagaagtag tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt atcagtccat
    18301 ttgttttctt ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc ttcttcgagt
    18361 ttacgacgcg cttcttcttc agctttacgc ttgagctctg cagcttcctg ctcacggcgg
    18421 gctttttcag cctgggcgtt tttagtcata tcgtcttgtt gattgctcac tttgtctttt
    18481 tccgcagctt cacgtttcgc ttgttcagca gcttcacgct tagcttgttc tgcggcctca
    18541 cgttcagctt tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg acgggcttgc
    18601 tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt gcgtttcttg
    18661 cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt aagggtgctg
    18721 cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt caggtggtca
    18781 atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg gatacctgca
    18841 tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc cagcgtttta
    18901 atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc gtcaccgaac
    18961 cagcaaatat tacgggcagc cataatcagt gctccggctt tttcgtcggt caacccttcg
    19021 atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca aacgccacgg
    19081 gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag atcgtcagcc
    19141 ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag tgcattttta
    19201 gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc caacagctct
    19261 ttcatcggca cataggccag ttcttccagc gtcgagaaac cttcttctac cagaacagtc
    19321 gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc gtgcgcttcc
    19381 gcctgatgct tagcctgcag gtcgtcaacg gtcattacgt tgagttccca accgctcagc
    19441 tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag gttaccggct
    19501 tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc aacgtctgcc
    19561 ggtgccattg cgttaatcac aaactgcgcc gggttatcat cccacaggac gatatcgata
    19621 cgctcgccgc ccagttcagt agaaaccgcc tgaacacgcg cgccacgcat acctacgcaa
    19681 gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc acgagaaccc
    19741 gggtcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttccgg cacttcaata
    19801 cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg cgcgccacgc
    19861 gcttccgggc gaacggaata aagcacgcca cgaacgcggt cgccagggcg gaagttttca
    19921 cgcggcagca tatcttcgcg caggatcacg gcttcagcat tgttgcccag atccagagag
    19981 atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc gtgttcacgg
    20041 aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat aacctgtttt
    20101 gccgtctggg tggtgatacg gtcaaaggta acagactcaa tctgatcttc aacgtaatcg
    20161 cccaagttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc cttggtcggc
    20221 tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc gcttttgcga
    20281 tcgatctgta cacggacgtc gatctcttgc tcatattttt tctttgttgc tgtcgccagc
    20341 gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt ggatacggct
    20401 tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc agactattaa
    20461 aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc atctttacct
    20521 tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg ccatttacga
    20581 cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa acgggcgtag
    20641 tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag gttataagca
    20701 acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac atcagcacaa
    20761 tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga tgtgcgaccg
    20821 cgaataaatt cgatgcctac cagttcaaaa cccagggcct caactggcgc agtaatcatc
    20881 tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa aaagggccta
    20941 aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg ctttatataa
    21001 ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt tttcaaatcc
    21061 cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg gaaagtggtt
    21121 gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc taccagactg
    21181 ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa atgcaaatac
    21241 tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata gtgatccatg
    21301 ccacattttg tcaacgttta ttgctaatca cgtgaatgaa tatccagttc actttcattt
    21361 gttgaatact tttgccttcc cctgctctcc cttaagcgca ttattttaca aaaaacacac
    21421 taaactcttc ctgtctccga taaaagatga ttaaatgaaa actcatttat tttgcataaa
    21481 aattcagtga gagcggaaat ccaggctcat catcagttaa ttaagcaggg tgttatttta
    21541 tgacgacgat tctcaagcat ctcccggtag gtcaacgtat tggtatcgct ttttccggcg
    21601 gtctggacac cagtgccgca ctgctgtgga tgcgacaaaa gggagcggtt ccttatgcat
    21661 atactgcaaa cctaggccag ccagacgaag aggattatga tgcgatccct cgtcgtgcca
    21721 tggaatacgg cgcggagaac gcacgtctga tcgactgccg caaacaactg gtggccgaag
    21781 gtattgccgc tattcagtgt ggcgcatttc ataacaccac cggcggcctg acctatttca
    21841 acacgacgcc gctgggccgc gccgtgactg gtaccatgct ggttgctgcg atgaaagaag
    21901 atggcgtgaa tatctggggt gacggtagca cctataaagg aaacgatatc gaacgtttct
    21961 atcgttatgg tctgctgacc aatgctgaac tgcagattta caaaccgtgg cttgatactg
    22021 actttattga tgaactgggc ggccgtcatg agatgtctga atttatgatt gcctgcggtt
    22081 tcgactacaa aatgtctgtc gaaaaagcct actccacaga ctccaacatg cttggtgcaa
    22141 cgcatgaagc gaaggatctg gaatacctca actccagcgt caaaatcgtc aacccgatta
    22201 tgggcgtgaa attctgggat gagagcgtga agatcccggc agaagaagtc acagtacgct
    22261 ttgagcaagg tcatccggtt gcgctgaacg gtaaaacctt tagcgacgac gtagaaatga
    22321 tgctggaagc taaccgcatc ggcggtcgtc acggcctggg catgagcgac cagattgaga
    22381 accgtatcat cgaagcgaaa agccgtggta tttacgaagc tccggggatg gcactgctgc
    22441 acattgcgta tgaacgtctg ttgaccggta ttcacaacga agacaccatt gagcagtatc
    22501 acgcgcatgg tcgtcagttg ggccgtctgc tgtaccaggg gcgttggttt gactcccagg
    22561 cgctgatgct gcgtgactct ctgcaacgct gggttgccag ccagatcact ggtgaagtta
    22621 ccctggagct gcgccgtggg aacgattatt caatcctgaa taccgtctca gagaacctga
    22681 cctacaagcc agagcgtctg acgatggaaa aaggcgactc ggtgttctcg ccagatgatc
    22741 gtattggtca attgaccatg cgtaatctgg atatcactga tacccgagag aaactttttg
    22801 gttatgccaa aactggcctg ctttcctcct ctgccacttc aggcctgccg caggtggaga
    22861 atatggaaaa caaaggccag taattcgaca tcaaccctgc ccttcagggg tggggttacc
    22921 ttgcttatcc tctgtaaact cctttctgag catcacataa tcgaaaacat catggtttgc
    22981 atccacaacc cgtgagtctc ctttatagtg tgcaatcact tcctcaagcg tttgcggctg
    23041 ttccggcttt gttaccccca tccacgcatt aataaggtaa ttattgtaag ctgtcgaaaa
    23101 gatattgttt tccgttgtgc gatccacgcc atccccaaga acggggctat aacagataaa
    23161 catcgggaca tgatatgcct gctggctagc ctccctgcct ccatgaaaat agacgttctt
    23221 cttcgtaggg tcacgttcca ggccgtggtc ggcaaaatac atgaccgagg cgcggcgatc
    23281 ttttaataat tcaaaaaact gacctagcaa actatctgtg taatgaatgg agttgtcata
    23341 gcaggcatcc tgatcgtcct gcggttgaaa cacgcgctgg attgcggata ggcgctacaa
    23401 gccggttcat ggcttccatt taaatgaaga acaatcagtt tcttttgctg cgtattttgc
    23461 tgtaatgctt gcgataaatg cggcaacaac aattcatcaa atcctctgac ataaactgtt
    23521 tccatggcgc gcatggcgat actggtaact gctgtaccat tctgccgaaa agcggattgc
    23581 gagcttagcc agaaagtctg aaatcctgct tgattagcca tattaataat gttgtcgggg
    23641 taattatgaa tgtcatgact caaaacagaa tcagcagtta aagaaagggg aaccgacagc
    23701 gcagtgtaag gtgcgccgct tattgcttga ttaaacagtt tgatctgttt tctttgtgct
    23761 tcaacttgcg gtgtcgtaga gcgtgtatat ccatacaaag acatattgtc gacacgtaca
    23821 gactccccca caatcaacac gtaggtatca atacttgtat ccctgactga taattgaaaa
    23881 tacggcaccg tgtttgcaat tgagagtaat ctttgatgct ctttcgctgc taaagcaaaa
    23941 tagttgagat tgaaaaacgg cgtataggta gcaaatcacg acgctagtat atatggactg
    24001 aacgcttttt tattttttgc atctttataa gcaaattgac aagcggaaaa taaactgccc
    24061 gaaatgacaa tcagcaataa tattcctgtc actttttttg tcggcaagga aacatcatat
    24121 tttattatta ctgccaaaaa aaaaagggat aaaaaagcaa aagcacatag ataagggata
    24181 tacatcccca gcattttcac aacttcatcc ggatcactct gcagcacact aatcgcaaat
    24241 ccatcattga aagttgtacc aaaggtacac catgaataga ggctaatact catgtctgcc
    24301 gcagtcagta acacaaatgg tgcggcaaca agcaggcgaa gcggaaggga tgatatacgt
    24361 ttaacaacca ggatggtcag gacgaagaac agcaatcgct gaaccatatg cgctccggaa
    24421 gaggcgacta agttcgtaat accaaaaaca ataacacaaa gatacaacaa ccaatgagat
    24481 ttaaaatctt ctagcaaatt tgttgaatac tgtcatccag aaatccaagt tcacaaccaa
    24541 tataaacaat tatcaacaat gttcaatttt atatcagaca tgtttttcaa ctctgaacca
    24601 tatcagaccg aaacactgtt agttttttct ttttaaatga tgcgaccatt gcgttctcag
    24661 taattaaata acattatcaa tgcgtctcta aacattacgc agaacgtagc catcagtagt
    24721 tatactgaca ggagcaggat ggtttatcga gaagtaaaca tatccgtatt cgtaccggaa
    24781 ctggagttga cacaataaag tgccaattat gtcagcagta gggaaaagtt tataactaag
    24841 gcgtaccggc accatcgttt caaggtacca gctacgagta aagcaactgg acgagataca
    24901 gatacctgac aaccattcat cagaccagga ccaaaacgaa aagagacgct tttcagcgtc
    24961 tcttttctgg aatttggtac cgaggacggg acttgaaccc gtaagcccta ttgggcacta
    25021 ccacctcaag gtagcgtgtc taccaattcc accacctcgg cacggatact actttttagt
    25081 tcgggatatc gctggtcggc ttagccggag cagctggctg agtttgttcg gttttcgccg
    25141 gtgcactcag attttcccat tcgctacctc tattggtttt gttgctattg atgttaccca
    25201 gcaccagact gatgatgaag aataacgttg ccagcagcgc cgtcatgcgg gtcatgaagt
    25261 taccagaacc acttgaacca aacagcgtag cggaagcgcc tgctccgaag gaggctccca
    25321 tatcagcgcc tttaccttgc tgcagcatga tcagaccaac aaggccaatt gccacaataa
    25381 ggaaaactac taaaagagct tcgtacataa tcaacctgtt ccttgcggag ttgccgcgta
    25441 ccaatgcttc aaccaataaa gcgggagttt ttaatctttc ccactgaagc gggtgtgaat
    25501 actaaccaaa gcgaatgacc ttcgcaaggg caattttatc gcattgtatc aactgcggaa
    25561 aaaaacagca aaaccgatgt gttcgccgag aaaaaaggcg acaatcgccg cctttttagc
    25621 cagttatcta acgctttaaa cggcttttac tgcatcggcg atgcggtgtg caaattcagt
    25681 cacctgcgct tcgtcttcgc cttccaccat cacgcgaatt aacggttcgg tgccggattt
    25741 acgcagcaac acgcgtccac ggttgcccag cgcagcttca acctctgcgg tcacggcttt
    25801 aactgactca tgctcaagcg gatcgccgct acctgcggtg taacgtacgt taaccagaat
    25861 ctgcgggaac attttcatgc cgctgcaaag gtcgtgcagg ctcatatggt tacgtgccat
    25921 cgccgccagc acctgcaagc cagcaacgat gccgtcaccg gtagtggttt tatccagcag
    25981 gatcacatga ccggaattct ctgcaccgat acgccagcct ttctcctgca ttttttccag
    26041 tacgtagcgg tcacccactt tcgcgcgcgc aaatggaatt cctaactgtt tcagcgccag
    26101 ttcaagcccc atgttgctca tcaatgtacc cacagcgcca ccacgcagct ggccctgacg
    26161 aagaccttca cgcgcgatga tatacatgat ctgatcgcca tcgactttat tgccttcatg
    26221 gtcaaccata atcacgcgat cgccgtcgcc gtcgaaggca ataccgagat ccgctttttc
    26281 agccagcaca cgagcctgga gcacgcgaac gtcggtagcc cccacttcgg cattgatgtt
    26341 tacaccgttt ggctcacaac cgatagcgat aacgttcgcc cccagttcgc gcagcacgtt
    26401 cggcgcgatg tgataagtcg caccgtttgc acaatccacc acaatcttca gttcactgag
    26461 gctaagttcg ttcgggaacg tggctttgca aaactcgata tagcgacccg cggcatcaac
    26521 gatacggctg gctttaccca gttctgccga atcaacgcag ctgatctcct tttccatttc
    26581 cgcttcgatg gcctcttcta ccgcatccgg cagtttagtg ccgtcgatag agaagaattt
    26641 aatcccatta tcgtagaacg ggttatgcga tgcagatatc acaattccgg cctctgcgcg
    26701 gaaggtacgc gtcagataag ccacggccgg tgttggcatc gggccagtga agagtgcgga
    26761 aaggcccgct gccgccagac ccgcttccag tgctgactcc agcatatagc cagaaatacg
    26821 cgtgtcttta ccaataataa tcttacggga gccgtggcgc gccagcactt tacccgcggc
    26881 ccaacccagc ttaagcacaa aatcaggtgt gatcggcgca tcccctacac gaccacgaat
    26941 cccatcggta ccgaaatatt tacgattact catagcgttt gttttccttt gcagacagag
    27001 tggcttccac cacccgcatc gcttctacgg tttctttgac gtcatgaaca cgaatgatgt
    27061 gcgcgccttg cattgcggca atgaccgcac aggccagact accgctcagg cgctcggacg
    27121 gccccacgtt cagcagctgc ccaatcatcg attttcgtga catacccacc aacagcggca
    27181 ggttgaaatg gtgaaattca gccaggcgcg ccagtaatga atagttatgg gagagatttt
    27241 taccgaaacc gaatccgggg tcgagcaaca atttctcttt tgcgataccc gcctgctcgc
    27301 aacgtgctat ttgctcaata aagtagcgat tcacttctgc aaagacatcg tcatacttcg
    27361 gagcttcctg catggttttt ggatttccct gcatatgcat cagacaaacc ggtaaaccgg
    27421 tttctgcagc cgcctccaga gcgccaggtt cggaaaggga gcggatatca ttaataatgt
    27481 gagcgccaac tttcgctgac tcacggatga cttctggttt ggatgtatcg accgagatcc
    27541 agacttcgaa gcgttgagca attgcctcaa ccacaggaat aacacgttgc aactcttctt
    27601 caacgctaac ttccgccgcc cctgggcgcg tggactcgcc accaacgtca atgatcgtcg
    27661 cgccagcatt gatcatcaga ttcgcatgtt tcaccgcatc tatcagcgag ttatgcgtgc
    27721 caccatccga aaaggaatca ggcgtgacgt tgaggatccc cattacgtga ggatggctaa
    27781 ggtccagtga agtaccctgg gcaaagagtt tcatgatgtt atccctggta tgaattgata
    27841 agaaaaaacc ccggagcacg ccccggggtt ttcggtacaa atacagtcat ctgatgcggg
    27901 aacttacttg tcgcctaact gctctgacat ggtgttaccc gggttcggcg tacgcggttc
    27961 atcaaccgga cgaggagcct ttggactacc attgtcgcca gaattgttag aagcgcctgg
    28021 ttcttcccag cccgctggcg gacgtacatc gcgacgtgcc atcaggtcat caatctgcgg
    28081 tgcgtcgata gtctcatatt tcatgagagc atctttcatc gcatgcagaa tatccatatt
    28141 gtcggtcaga agctgacgcg cacgattata gttacgctca atcagtgctt tcacttcctg
    28201 gtcgatgata cgtgcagttt catcggacat atgtttcgct ttcgctacgc tacggccgag
    28261 gaatacttca ccttcttctt ccgcgtacag cagtggaccc aatttctcag agaagcccca
    28321 ctgagtcacc atgttacgtg ccaggttggt cgcaacttta atatcgttgg acgcaccggt
    28381 agaaacatgt tccggcccgt agatgatctc ttctgccaga cgaccaccgt acagcgtaga
    28441 aatctggctt tccagtttct gacggctggc gctgattgcg tcgccctcag gcaagaagaa
    28501 agtcacaccc agcgcacgac cgcgtgggat aatcgtcact ttatgcaccg gatcgtgttc
    28561 cggcaccagg cgaccgataa tcgcatgacc cgcttcgtgg taagccgttg attctttctg
    28621 cgcttccgtc atcaccatgg agcgacgttc cgcacccatc atgattttgt ctttcgcttt
    28681 ctcgaactca accatcgaca caacgcgttt gttgccacga gcagcgaaca gtgccgcttc
    28741 gttcaccagg ttcgccaggt cagcaccgga gaaaccagga gtaccacggg caatgattgc
    28801 cgcgtcgata tcgggtgcca atggtacgcg acgcatgtga actttcagga tctgctcacg
    28861 accgcgaaca tctggcaagc cgaccacaac ctggcggtcg aaacggccag gacgcaacag
    28921 ggccgggtcg agaacgtccg gacggttagt cgcggcgata acgatgatac cttcgttacc
    28981 ttcgaagcca tccatctcaa ccagcatctg gttcagagtc tgttcacgtt catcgtgacc
    29041 accgcccaga ccagcgccac gctggcggcc tacggcgtcg atttcatcga taaagatgat
    29101 gcacggtgcc gctttcttcg cctgttcgaa catgtcacga acacgggatg cacccacacc
    29161 gacgaacatt tctacgaagt cagaaccgga gatagtaaag aacggaactt tcgcttcgcc
    29221 tgcaatcgct ttcgccagca acgttttacc ggtacccgga ggaccgacca tcaggacgcc
    29281 tttcgggatc ttaccgccga gtttctggaa gcggctcggc tcgcgcagat actctaccag
    29341 ttcagcaact tcttcttttg cttcgtcgca gcccgcaacg tcagcaaagg tcgttttgat
    29401 ctgatcttcc gtcagcatgc gcgctttgct cttaccaaac gacatggcac ctttgccacc
    29461 gccgccctgc atttgacgca tgaagaagat ccagacacca atcagcaaca gcatcgggaa
    29521 ccaggagatg aagatagaag ccagcaggct tggttcttca ggcggttcac cgacaacctt
    29581 gacgttcttg gtcaacaggt tatccagtaa tttcggatcc tgaaccggaa tgtaagtggt
    29641 ataacggtta ctatctttct tggtaacgtt gatttcacgt ccgttgatac gcgcttcacg
    29701 aacctggtcg ttattgacct cttgtaggaa ggtagagtaa tccaccttac ggccattaga
    29761 ctcgctgggc ccaaagctct ggaatactga catcagcaca acggcaatga ccagccagag
    29821 tattaggttt ttcgccatgt cactcaaggg attaacctct tattacaact gtgttaaaaa
    29881 cagcgtcagg atactctata tccagcatct ttcaaacttt cgtctgaaat ctcccggtta
    29941 gggtttacgc ccggtcgcta caatatacac ttctcgcgaa cgtgcacgag aagagtccgg
    30001 cttacgaact ttgaccttcg taaacaggga gcgaatttcc cttagatact catcgaaacc
    30061 ttcgccctgg aacaccttca ctacaaaact gccacctggc gctaatacat cacgacacat
    30121 ttctagcgcc agttccacca gatacatggc acgggggata tccaccgccg gtgttccgct
    30181 catgtttggt gccatatcgg acatgacaac ctggactttg ctgtcgccaa cgcgctccag
    30241 cagtgctttc atcaccagtt catcacgaaa atcgccctga agaaagtcca caccaacgat
    30301 aggatccata ggtaaaagat cgcaagcgat gatgcggcct ttgccgccaa tttgggtgac
    30361 cacatattgt gaccaaccac ccggagcagc accaaggtcg acaaccgtca ttcccggttt
    30421 aaagagtttg tcactttgct gtatttcatc aagtttaaac caggcacggg aacgtaatcc
    30481 ctttttctgt gcctgttgaa catatttatc gctaaagtgt tcctgaagcc agcggctgga
    30541 gctggcagaa cgcttcttac ctgtcattta actttcccat ggggataact catcgtaacc
    30601 aattgcgtaa atttttacgc gcctatttgg tgatatatgg gagatggcgg tagaatgacc
    30661 cgttttcaat cccaacgtaa gcaaaaaata cgatgaatct gagtactaaa caaaaacagc
    30721 acctgaaagg tctggcacat ccgctcaagc cagttgttct gcttggcagt aatggtttga
    30781 ccgaaggggt gctggccgag attgaacaag cgttagagca ccatgaactc atcaaggtga
    30841 aaatcgccac cgaagatcgc gaaacaaaaa ccttgatcgt ggaagctatc gtgcgcgaaa
    30901 ccggcgcctg taatgtacag gtcatcggta aaacgctggt gctttatcgc ccaactaaaa
    30961 aacgtaaaat ctcgctgcca cgctaagatt atcctaaagt tacacacatt tgctgtgtaa
    31021 aacgaggggt tttccgcagg caggagagca aaatgccacg ctctgttcgt tgataaaagg
    31081 ccgcagagcg gcctttttcc tttctttaca atacatcaac atcttgagta ttgggtaatt
    31141 cttacaggta ttccacctta attacttcaa attctacttc gccgcccggc gttttgatga
    31201 ccacaacatc atcttcttct ttgccgatca ggccacgagc aataggcgag tttacagaaa
    31261 tcaggttttg tttaaagtca gcttcgtcat cgccaacgat gcgataagtc tgttcttcgt
    31321 cagaatccag attcagcacc gttacggtag caccaaaaat aacgcgccca ttgttgggca
    31381 ttttggtgac atcaatcacc tgcgcgtttg acagcttggc ttcgatgtct ttgatacggc
    31441 cttcgcagaa gccctgctgt tcacgagctg cgtggtattc ggcgttttct ttcaggtcgc
    31501 catgctcacg cgcttccgcg atagcagcaa tgatttcagg acggcgcaca gatttcagaa
    31561 aatccagctc ttcgcgtaat ttttcagcgc cgcgtaaggt catcggaata gcttgcattt
    31621 gttatacctc ttgaatattc ctgatggggc aagtcttcac ccaccccggc tgttcggcct
    31681 gcccggcata actcctcacc gggaccagaa gcaaaaaaat accgacccgg gtacaagtcc
    31741 caggtcagct acaattcaca ttttgatggt cattttaccc tgaagttccc gaagggtcat
    31801 cgtttacttt atagggcgtt gcgccgtagt atgacggctc gattccaggt tgttagcgcg
    31861 agattatgcg attttccaga tttatcatcg gattgaccag ctgtatagcg ttcagtgttc
    31921 aggccgcgaa tgttgatgag tacgttactc aactccccgc aggtgccaac cttgccctga
    31981 tggtgcaaaa agtcggtgcg tcggcccccg ctattgatta ccacagtcag cagatggcgc
    32041 tgcccgccag tacccagaaa gtgattactg cgctggcggc gttgattcaa ctcggccccg
    32101 attttcgttt caccacgacg cttgaaacca aaggcaatgt ggaaaacggc gtacttaagg
    32161 gtgacttagt ggcgcgattt ggtgccgatc cgacgttaaa acgtcaggat attcgcaata
    32221 tggtcgcgac tttgaaaaaa tctggcgtca accaaatcga tggcaatgtg ttgatagata
    32281 cctccatttt cgccagccac gataaagccc ccggctggcc atggaatgac atgacacaat
    32341 gctttagcgc tccgcctgct gccgccatag ttgaccgcaa ctgtttctcc gtctcgctct
    32401 acagtgcccc aaagcctggt gatatggctt ttatacgcgt ggcatcttat taccccgtta
    32461 cgatgttcag ccaggtacgc accctccccc gtggttctgc cgaagcgcaa tactgcgaac
    32521 tggatgtggt gccaggcgac ctgaaccgct ttacgctgac gggatgcctg ccacaacgtt
    32581 ctgagccgct cccgttggct tttggcgtgc aggatggagc cagctatgcc ggtgcaattc
    32641 tgaaagatga gttaaaacag gcgggtatca cctggagcgg aacactgctg cgccagactc
    32701 aggttaacga acctggaacg gtagttgcca gtaaacagtc ggccccgctg cacgatctgc
    32761 ttaagattat gctgaaaaag tcggacaaca tgatcgccga tacggttttc cgcatgatcg
    32821 gccatgcgcg cttcaatgtg cctggaacat ggcgggctgg gtcggacgcc gtgcgtcaga
    32881 tcctgcgcca gcaagccggt gtcgatattg gtaacaccat tattgccgat ggttcagggc
    32941 tttcgcggca taacctgatt gcccccgcca ccatgatgca ggtgctgcaa tacattgccc
    33001 aacacgacaa tgaacttaac tttatctcca tgctgccgct tgcgggctat gacggctctt
    33061 tgcagtaccg tgcaggtctg catcaggcgg gcgtggatgg caaagtctca gcgaaaaccg
    33121 gttcgttgca gggggtatat aacctggcgg gattcattac cacagcgagc gggcaacgaa
    33181 tggcatttgt acaatatctt tctggctatg cagtaaaacc cgcggatcag cgtaatcgcc
    33241 gtattccgtt agtgcgtttt gaaagccgtt tgtacaaaga tatttatcag aacaattagt
    33301 caaaaagaaa ccccggcgca tggccggggt ttcagattat tgacaaagtg cgctttgttt
    33361 atgccggatg cggcgtgaac gccttatccg gcctacaaaa tcgtgcaaat tcaatatatt
    33421 gcaatcatct tgtaggcctg ataagtgtag cgcatcaggc ttatttggca ttggtcatta
    33481 gtgattaacg cttgtaaatg aactcaacgc cttcttcgtc gtcttcgtcc cagtcgtcat
    33541 cccagtcttc atcatcctct tcagcaatct cttcaagctg ctgacggtga taatcatccc
    33601 acatgaattc gactttctct ggctgtttcg cttcttcagc ctgcacgacc gggttttcaa
    33661 tgataaaggt catcacatcc cagcagagat ctttcacgcc cagtccgctc gccgcagaga
    33721 tcagataata tttatcttcc cagcccagcg cttcagcgat cgctttcgct ttctcttcgg
    33781 cttctgcctt atccagcaga tcgatcttgt tgaagactaa ccaacgcggt ttcgccgcca
    33841 gatcctggct gtatttttcc agctcgctga taataatacg cgcgttttca accggatcgg
    33901 tgccgtcaat cggatcgata tcgatgaggt gcaacaggac gcggcaacgt tccaggtgct
    33961 tcaggaagcg aatgcccaga cctgcgcctt ccgcagcgcc ttcaatcagt cctggaatat
    34021 cggcaacaac gaagctcttt tcgttgtcca ttcgtaccac acccagactt ggcaccagag
    34081 tggtaaacgg ataatccgcc actttcggtt tagccgccga taccgcacga ataaaggtcg
    34141 atttacccgc gtttggcatc cccaacatac cgacgtcagc cagcagcatc agctccagca
    34201 gcagctcacg cttatcgccc ggcgtaccgt tagttttctg ccgtggtgta cggttaactg
    34261 acgatttgaa acgggtattg cccagaccgt gccagccgcc cttagcaacc agcagacgct
    34321 gaccgtgttt agtcatatca cccatggttt caccagtacc ctggtcgatt acacgcgtac
    34381 ctaccggcac tttaatcgtc acgtctttac cgcgcttacc ggtacaatcg cggcttgcgc
    34441 cgttctgacc gcgctctgca cggaaagatt tttcaaaacg ataatcgata agcgtgttca
    34501 ggttctcgtc ggcttccatc catacgtcac caccatcacc gccgtcgccg ccatccgggc
    34561 cgcctttcgg aatatacttt tcgcggcgga agctcacgca accattaccg ccatcacctg
    34621 caacgaccag aatcgatgct tcatcaacaa acttcatttt attctccgta aatcattcgc
    34681 ctgcgcgggg ttgcgatacc accgttgtat gcttacgtaa tccgccccaa atacgatgac
    34741 caatggcgga atacatcgcg cccgcaacca cgacaaacgc accgagataa cctaaaaggt
    34801 ttaacatcgg tctggcgaag aaatcgggcc aggccagtga taaaagatct gaaaaaaaca
    34861 gcgtaaacag tggggtgagc gtgatgatcg cgctcacctg agctgcctgc cagcgagcca
    34921 tcgcttccgc caaggcgcca tatcctacca aggtattcag tccgcaaaaa attaaacatg
    34981 cgagctgcca gtggctaagc tgcgctatca ctccaggctt tgccagaggg aagagcgcga
    35041 ttgtacataa agtgtacagt aaaaacagga tctgcggtga ggccagccga cgcaataaaa
    35101 ccttttgcgc cacgccataa ctcacccaaa ccgtcgccgc accgacccca aagataactc
    35161 cccaggtgta atcggtgagc tttgtaaata tctcgaccag actggtgtta aaaaacatca
    35221 ccagaccgct caggagcatc aatgccccta caacctgagt gctgcgcatt ttctctttca
    35281 ggataaatac gctggcaacc atcatgccaa ctggcgagag ttgcccaatc acctgcgaag
    35341 cggtcggact caggtattgc aaggatgagc tgaacaggat gaagttccca aacagcccgg
    35401 cggtcgccac tgccaacaaa atcaaccagc gtggcttacg aaacacgcgt aatggcggca
    35461 acctcttctt caccgcaaga atggcaccaa ggccaatact cgccatcaag aaacggtaaa
    35521 acacgattgt cggaggttcc atcacctcca gcacctgctt cattgcgatt ggcaacgccc
    35581 cccagcaaat tgctgtggta agcgccaaaa gaatgccaat gcctgcctgc tgcttcatac
    35641 cgtattccct gcaaagcgca tttgcggatt aacgtcgcac catgcgcatt tttaccggtt
    35701 atcgaatgta aaaagccccg caacgtgttg cggggctttc atccgttacc gggacgcgaa
    35761 aaacttattc agcttcgatg ctgataaatt tacggttttt cgggccttta acttcgaatt
    35821 tcactttacc gtctgcttta gcaaacagag tgtggtcacg accgcaacct acgttagcgc
    35881 cagcgtggaa tttggtacca cgttgacgaa cgatgatgct acccgccaga acggattcgc
    35941 caccgaaacg cttaacgccc aggcgtttag cttctgaatc gcgaccgtta cgtgtggagc
    36001 cgccagcctt tttatgtgcc atttgaaatc tctcctcagg tcttaggcgc tgatgccagt
    36061 aattttcaca tcagtgaacc actgacgatg gccctgctgc ttacgatagt gtttacgacg
    36121 acgaaactta acgattttaa ctttctcgcc acgaccgtga gcaacaactt cagctttgat
    36181 tacgccgcca tcaacgaaag gaacgccgat tttgacttct tcaccgtttg cgatcatcag
    36241 cacttcagcg aactcaacag tttcgccagt tgcgatgtcc agcttttcca ggcgaacggt
    36301 ctgaccttcg cttactcggt gttgtttacc accactttgg aaaaccgcgt acatataaaa
    36361 ctccgcttcc gcgcacacct tttcaatgat tcaaagtgcg ctataaatat tcacaatagg
    36421 gcgcgaatat tacgcaaaac gcacgccttt gacaagtgct acagtcaata cacgaagaaa
    36481 aaaaacacaa cttgtacggt aacgtttatc tgtgccattt tttcagtaca attaccctat
    36541 attcctaacc ataaacccta agttgccttt gttcacagta aggtaatcgg ggcgaaaagc
    36601 ccggcttttg cgatgaattt agaaaaaatc aatgagttaa ccgcgcaaga tatggcgggt
    36661 gttaatgcgg caatccttga gcagcttaat tccgacgtcc aactgatcaa tcagttaggc
    36721 tattacatcg tcagcggcgg cggtaaacgt attcgtccga tgattgctgt actggctgca
    36781 cgagctgttg gctatgaggg aaatgcgcat gtcaccattg ctgccctgat cgagtttatc
    36841 cacacggcga ctctgctaca cgacgacgtt gtggatgaat cagatatgcg caggggtaaa
    36901 gctaccgcca acgccgcatt tggcaatgcc gccagcgtgc tggtaggcga ttttatttat
    36961 acccgcgcgt tccagatgat gaccagcctc ggttcactca aagtgctgga agtcatgtca
    37021 gaagctgtaa acgtcatcgc agaaggtgaa gttctgcaac tgatgaacgt taacgatccg
    37081 gacatcactg aagaaaacta catgcgcgtt atctatagca aaaccgcgcg tctgtttgag
    37141 gctgccgcgc agtgttccgg gattctggct ggctgtacgc cggaggagga gaaaggcctg
    37201 caggattatg ggcgctatct cggcactgct ttccagttga tcgacgattt actcgattac
    37261 aatgccgatg gcgaacagtt aggtaaaaac gtcggcgacg atctgaacga aggtaaaccg
    37321 acgctgccgc tgctgcatgc gatgcatcat ggcacaccag aacaggcaca gatgatccgt
    37381 accgccatcg aacaaggtaa cggtcgccat cttctggaac cggttctgga agcaatgaac
    37441 gcttgtggat ctcttgaatg gacgcgtcag cgcgccgagg aagaagcaga caaagccatc
    37501 gcagcgttac aggtgctccc ggacacccct tggcgagaag cactcatcgg cctcgcgcac
    37561 atcgctgttc aacgcgatcg ttaatcccct cccttcatcc cgcgcagtgc gcgggatgat
    37621 tccagtaaat tccataaaaa cactaattca gcgctataac acctgaaaaa aagggcgatg
    37681 aatctttata tattctgaat attcacactc tttacaggaa ctttttagcg caataggcca
    37741 tcaggagtat agtgatgccc gacagaagaa gtgttctgaa tgaaagcgac taacttaagg
    37801 agtgaggaaa gatggaaagt aatttcattg actggcatcc cgctgacatc attgcaggtt
    37861 tgcgcaagaa aggaacgtca atggcggcgg aatctcgcag aaatggtttg agttcctcaa
    37921 cgctggcaaa cgcattatcg cgcccatggc cgaaaggaga gatgattatt gcgaaagccc
    37981 tgggaactga cccctgggtt atctggccat cacgctacca tgatccgcag acccatgagt
    38041 ttatcgacag aacgcagttg atgcgtagct acactaaacc gaaaaaatga gtggtctggc
    38101 ggtagccccg cgaacggggc tgccagctct cagacgatta ctcgcctttc acacgctcaa
    38161 tatttgcacc taaagcgcgc agtttgtctt caatgcgttc gtagccacga tcgatgtgat
    38221 aaatacgatc aaccaccgtc gtcccttccg caatacagcc agccagcacc aggcttgctg
    38281 atgcacgcag atcggttgcc ataacctgtg caccagaaag tttttcaaca ccatgacaaa
    38341 taacggtatt gctttcgatt tcggcgtgcg cgcccatacg gctcagctct ggcacatgca
    38401 taaagcggtt ttcaaagacc gtttcggtga tgaacccggt cccttcagcc accaggttca
    38461 acagcgtgaa ctgggcctgc atatcggtcg ggaatgccgg atgcggcgcg gtacgtacgt
    38521 taacagcctt cggacgtttg ccatgcatat ccaggctaat ccagtcttcg ccgacttcga
    38581 tgtccgctcc agcatcacgc agtttcgcca gcacggcgtc cagagtatct ggctgcgcgt
    38641 tacggcagat aattttgcca cgagagatcg ccgccgccac caggaaagta ccggtttcga
    38701 tacgatccgg cagcacgcga tagacaccgc cgcctaaacg ttccacacct tcgatgacga
    38761 tacgatcggt gccctgaccg ctaattttcg cacccagcgt aatcaggaag ttcgcggtat
    38821 cgacgatttc cggttcacgc gctgcgtttt caataatcgt ggtgccttcc gccagggttg
    38881 cagcacacat gatggtcacc gttgcgccaa cgctgacttt atccatcacg atatgtgcgc
    38941 ctttcagacg accatcgacg gaagctttaa cgtaaccttc ttccagtttg atggtcgcgc
    39001 ctaattgttc gaggccagaa atgtgtagat caaccggacg agcaccgatc gtacaaccgc
    39061 caggtagtga aacttgcccc tgaccaaagc gcgctaccag cggccccagc gcccagatag
    39121 aagcacgcat ggttttaacc agatcgtaag gtgcgcagaa tacattaacg tcgcgggcat
    39181 caatatgcac agaaccatta cgttctactt tcgcacccag ctggcttagc agtttcattg
    39241 atgtatcgac gtctttcagt ttcgggacgt tctggatctc taccggttct tccgccagta
    39301 gtgcggcaaa aagaataggc agagcagcat ttttagcgcc ggaaattgtg acttcgccct
    39361 ggagcttcgt tggcccctga acacgaaatt tatccattta gtttgttctc agttaacaat
    39421 tcatatccgc taccggcgaa tcgcccatag ctcaaaagcc gttcagtttg cgatcgcgcg
    39481 cccactccgc aggggtatac gctttgatcg acacagcatg aatgcggtta tccgcaatat
    39541 attccatcag cgggccatag accgtctgct gttttttaac ccgactcata ccgtcaaaca
    39601 actcacccac ggcaataacc tgaaagtgac tgccatcgcc ggaaacgtgg acttcctgga
    39661 gggagagagc gttcatcaac acgctctgaa tttcattatt ttccatggga tcttcaatca
    39721 tcagttaata aaccagcgaa acatcttaga gcaaagttgc gctggcataa ataagcaaaa
    39781 agcctcgctg ataaatcaga caaggctcga cttgcaggca ggtttgccgg acaggcggtt
    39841 aacgccatat ccggcctgaa aaaatttaac gaggcagaac atcagcaggc aaattataca
    39901 atttcgcgag ggtatacact ttgtcgttta ccccctgaag cgtcacgttg ttgccctgct
    39961 ttttcgccag atcgataaga tggagcagca gtgccagtcc ccccgtatcc acgcgggaca
    40021 cacggctaag atcgatgcag gtaatcccct tcaccgcttc ctcacgcatt tcccaaagcg
    40081 gtagcagaac gtcctgatcc agctctccgg ataacgccag cgtgtcaccc gtctgcatcc
    40141 agctcagtga ctcgctcatt attttttctc ttccagagtg attttctgtt gagaaatcga
    40201 tttcagttgc gcagtcaggc cgtcgatacc tttggtacgc agcagcgttc cccactcgtt
    40261 ttgtttggtg gtgatcatac tgacgccttc agcaatcatg tcgtaagctt gccagtttcc
    40321 cgtctgggag tttttacgcc actggaagtc cagacgcacc ggcggacggc cattcgggtc
    40381 aataatggta acgcgaatag gcacaatggt tttatcgccc agcggctgtt ctggcgcaat
    40441 ctgataggtt tgaccgtgat acatcgccag cgcctgaccg taagcctgct tcaggtactc
    40501 acggaaagcg gcaaagtagg cttcacgttg agcaggggtc gcactcttgt aatactggcc
    40561 cagcaccagc gcaccggcgt atttcacctg tacgtatggc agcagttcct gatcaacaat
    40621 ggtacgcaga taatccgggt tggcccgaat ttgcggttgc tcattcttca ggcgatcgaa
    40681 cgttttctgc gccgcctcgt ccatcagctt ataaggattg gtctggtctg ccgcggttgc
    40741 cgcactcaga ggtgcaatca ccagcaaagc gaccatcatt aaacgtttaa acatgcgtcg
    40801 gttctcctga aattatttcg ttgtacccac aggttcagtg gtttcattat tacctggcgc
    40861 agcagctggc gcatcgccac tattcttatt gtcatcgcct ttactaccgt aaaggaactg
    40921 accaatgaga tcttccagca ccatcgcaga cttggtgtcc tgaattgtat cgccatcctt
    40981 caggatagca gtccccagtt ccgggtcttc aaaaccgacg tttaatgcca gatattgttc
    41041 tcccagcagg ccggaagtac gaatgctcag cgaactggta tcaggaatgt ggttataacg
    41101 ttgttcaatt tccagcgtta cgcgcggcag ataggttttc gggtccagcg taatatccgc
    41161 cacccggccc acaacaacac caccaatgct gaccggagag cgcgctttca ggccgccaat
    41221 gttatcgaac gtcgcataaa gcgtgtaggt cgattctgta cgtatggacg tcacgttcgc
    41281 cgccttcagg caaacaaaca gcgccgccag cagtgctgct aataaaaaga tacccaccca
    41341 aatttcattt tttttcgttt gcatgaactc aattcccaaa catcaatgcg gtcagcacaa
    41401 aatccagccc cagaacagcc agagacgagt ggacaacggt gcgagtggtt gcccggctaa
    41461 tcccggcaga cgtcgggatg gcgtcgtagc cgttaaacaa cgaaatccac gtcaccgtga
    41521 tggcgaacac cacgctctta atcagacagt tgaccagatc catacgccag tcgacggcat
    41581 tttgcattgc cgaccagaag aacccgctat caatgccttt ccaactgacg ccgaccagcg
    41641 atccgcccca gatccccacg gcgacgaaga taaccgtcaa cagtggtaat gaaataaccc
    41701 cagcccagaa acggggagaa ataacccgac gcagcggatc caccgccatc atctccatac
    41761 tggagagttg ctctgtagcg cgcatcaggc cgatttctgc ggttaacgcc gaaccagcac
    41821 gcccggcaaa caacagcgcg gcaaccaccg gccccagttc acgcagtagc gataacgcca
    41881 ccagcatacc cagactggtt tccgcgctat aagtggtcag aaccagataa ccttgcagcc
    41941 ccaacaccat tccgatgaac acgccagaaa ctacaataat cagcatcgac aggacgccga
    42001 cattatagag ctggcgcacc agcagcggcg catgtttgcg aaattccggt ttgccgacca
    42061 gcgcattgaa taacattaac ccggcccgcc cgaacgttct cagggtttta atccctttat
    42121 gtccgagcga cgccagcgca tttaacagca tgagtggctt aactccctgg taaaagatca
    42181 gcgtgataat cgccggcagg atagcggaac ggaacaggcc cgtcagctat cccgtccaga
    42241 aactgacgta cgcgcggatc aggattcgtc tgcaacgcct gggcactgcc atgagcgaca
    42301 atttttttgt ccgccaggat ccaggcgtga tccgcaatac ttaacacttc cggcacatcg
    42361 tgagaaacca ccacacaagt cacgcccagc gcgctgttca gctcagaaat cagcttcacc
    42421 agtacgccca tggtgatggg atcttgccca acaaaaggtt catcaaacat gatgagatcc
    42481 ggctccagcg caatcgcacg cgccagcgct gcacgccgcg ccatcccacc ggaaagttca
    42541 gaaggcatta gtttagccgc tccacgcagc cccacggcct ccagcttcat catcaccgta
    42601 ctatgcaaca atggcgcggg aagttgggta tgttcgcgca gtggataggc gacgttgtca
    42661 aatacgttca tatcagtgaa caacgccccg gactgaaata acatgctcat ccgtttacgc
    42721 actgtataca ggcgcgaacg agacatcgcc ggaatattct caccatcgaa aaggatctca
    42781 ccatgatctg gtgcgatttg cccgccaatc agacggagta gcgtcgtttt accgatgccc
    42841 gatggcccca tgatcgccgt gatcttccct cgcggcacgg tcagggaaat attatcgaag
    42901 atgcagcgat tgccacgcgt aaaactgaca tcgcgcatat cgactaaatt cgccacagac
    42961 tgctccataa ttcacccttc gtcttgcgtt gattttctaa gcatggcgct caatttaacc
    43021 ttgaacccaa catatttaca gaatattacc cgccgtggtt agcgaaagct ggcatttgtt
    43081 ttacttttta gccgcataaa gtcaaaatta agaatccgtt acggctttct gaaaatcttc
    43141 agcggaccgg cgagtatacc tgaagaaagg acgttagatg cttttagcta cggcactgtt
    43201 aattgttggt ttacttttgg tcgtttacag tgccgaccgc ctggtttttg ccgcgtctat
    43261 tctttgccga acctttggca tcccgccgct gatcatcggc atgacggtgg tcagtattgg
    43321 tacatcgtta ccagaaatca tcgtctcgct tgccgcgtct ctgcacgaac aacgcgattt
    43381 agccgttggt acagccctcg gctcaaacat tatcaatata ttgctgatcc tcggtctggc
    43441 tgcgctggtt cgtcctttta ccgtccattc cgatgttctg cgccgtgaat tacccttaat
    43501 gttgttggtc agcgtggtgg ccggttccgt actctatgac ggacaactta gtcgaagcga
    43561 tggtatcttt ctcctctttc tggctgtgct atggctgctg ttcattgtta aacttgcacg
    43621 tcaggggact gacagcctga ccagagagca gcttgcagag ctgccgcgtg acggcggatt
    43681 gcccgtcgcg tttttatggc tcggcattgc gcttatcatc atgccagtgg ccacgcggat
    43741 ggtggttgat aacgccacgg tgctggcgaa ttactttgcc atcagcgagt tgacgatggg
    43801 tctgacggca attgctatcg gaaccagtct gccggaactg gcaaccgcaa tagcgggggt
    43861 tcgcaaaggt gaaaacgaca ttgctgtcgg aaatatcatt ggcgcaaaca tttttaatat
    43921 tgtcatcgtg ttgggtttac ccgcgctgat aacgccagga gagattgatc cactggcgta
    43981 cagtcgtgac tacagcgtga tgttgctggt gagcattatt tttgcgttgc tgtgctggcg
    44041 gcgctccccg caaccgggcc gtggtgtagg ggtattatta actggcggat ttatcatatg
    44101 gctggcgatg ttgtactggt tatcgccaat actcgttgaa taactggaaa cgcattatgt
    44161 cgcacgtaga gttacaaccg ggttttgact ttcagcaagc aggtaaagaa gtcctggcga
    44221 ttgaacgtga atgcctggcg gagcttgatc aatacatcaa tcagaatttc acgcttgcct
    44281 gtgaaaagat gttctggtgt aaagggaaag ttgtcgtcat ggggatgggg aaatcggggc
    44341 acatcgggcg caaaatggcc gcaacgtttg ccagcaccgg tacaccttca tttttcgtcc
    44401 atcctagtga agccgcgcat ggtgatttag gcatggtcac cccacaggat gtggtgattg
    44461 ctatctctaa ctcaggtgaa tccagcgaaa tcacggcctt aattccagtg cttaagcgtc
    44521 ttcacgtacc gttaatctgc atcaccggtc gcccggagag cagcatggcg cgcgccgcag
    44581 atgtgcatct gtgtgttaaa gtagcgaaag aagcctgtcc gttagggctg gcaccgacca
    44641 gcagcaccac cgccacgctg gttatgggcg atgccctcgc tgtcgcgctg ttaaaagcac
    44701 gcggctttac tgctgaagat tttgcgctct cacacccagg cggcgcactg ggtcgtaaac
    44761 ttctgctgcg cgtaaacgat attatgcata cgggcgatga gatcccgcat gttaagaaaa
    44821 cggccagtct gcgtgacgca ttgctggaag ttacccgcaa aaatcttggt atgactgtca
    44881 tttgcgatga caatatgatg attgaaggca tctttaccga cggtgattta cgccgtgtct
    44941 tcgatatggg cgtggatgtt cgtcagttaa gtattgccga tgtgatgacg ccggggggaa
    45001 tacgtgtgcg ccctggcatt ctggccgttg aggcactgaa cttaatgcag tcccgccata
    45061 tcacctccgt gatggttgcc gatggcgacc atttactcgg tgtgttacat atgcatgatt
    45121 tactgcgtgc aggcgtagtg taaagattca aggataaaca acaatgagca aagcaggtgc
    45181 gtcgcttgcg acctgttacg gccctgtcag cgccgacgtt atggcaaaag cagagaacat
    45241 tcgtctgctg atcctcgacg ttgatggcgt actgtcagat ggcctgattt atatgggcaa
    45301 taacggcgaa gagctgaaag cgttcaatgt tcgtgacggt tatggcattc gttgtgcgct
    45361 cacctctgat attgaagtcg ctatcattac cgggcgaaag gctaaactgg tagaagatcg
    45421 ttgtgccaca ttggggatca ctcacttgta tcaggggcag tcaaacaaac tgatcgcctt
    45481 tagcgatttg ctggaaaaat tggcgattgc cccggaaaat gtggcttatg ttggcgatga
    45541 tctcatcgac tgcccggtaa tggaaaaagt gggtttaagc gtcgccgtgg ccgatgccca
    45601 tccactgttg atcccgcgcg ccgattacgt gacgcgcatt gctggcggtc gtggcgcagt
    45661 gcgcgaagtt tgcgacttat tactcctggc gcagggcaaa ctggatgaag ccaaagggca
    45721 atcgatatga gtaaagccag acgttgggtt atcattgtgc tatcactggc ggttctggtg
    45781 atgatcggca ttaatatggc cgaaaaagac gataccgccc aggtggtcgt caacaacaat
    45841 gatcccacct ataaaagcga gcatacggac acgctcgtct ataacccaga aggggcacta
    45901 agctatcgat tgattgctca acacgttgaa tattattccg atcaggccgt ttcgtggttt
    45961 acgcagccgg tacttaccac gtttgataag gataaaatcc cgacatggtc cgtaaaagca
    46021 gataaagcca agctgaccaa tgaccggatg ctctatttat atggacacgt tgaagtcaac
    46081 gcacttgtgc cagactctca acttcgcaga atcacgacgg ataacgcgca gatcaatctg
    46141 gtgacgcagg atgttacctc tgaagacctc gtcacgttat acggaacaac atttaactcc
    46201 agcggtctga aaatgcgcgg caacttacgc agcaagaacg ccgagctgat tgaaaaggtt
    46261 agaacatcct atgaaattca aaacaaacaa actcagcctt aatcttgtgc ttgccagctc
    46321 acttctggcc gccagcattc cggcatttgc cgtaaccgga gacactgatc agccgatcca
    46381 cattgaatcg gaccagcaat ctcttgatat gcaaggcaac gtggttacct ttaccggtaa
    46441 tgtcatcgtc acccagggca ccatcaaaat taatgccgac aaagtggtcg ttacccgtcc
    46501 gggcggcgaa caaggtaaag aagtgattga cggatacggt aaaccggcaa cgttctacca
    46561 gatgcaggac aacggtaaac ccgttgaagg tcacgcttcc cagatgcact acgaactggc
    46621 aaaagatttt gtcgttctga cgggtaatgc ttatctgcag caggtcgata gcaacattaa
    46681 gggcgataag atcacttacc tggtgaaaga gcagaaaatg caggctttca gcgacaaagg
    46741 caagcgcgta accaccgttc tggtgccgtc gcagctgcag gacaaaaaca acaaaggcca
    46801 gaccccggca cagaagaagg gtaattaatt cgttatggca acattaactg caaagaacct
    46861 tgcaaaagcc tataaaggcc gtcgcgtggt agaagacgtc agcctgaccg tcaactccgg
    46921 ggaaattgtc ggtctgctgg ggccaaacgg tgccggtaag accaccactt tctacatggt
    46981 tgtaggcatt gtgccgcgcg atgcgggcaa catcattatt gatgatgacg atatcagtct
    47041 gctgcctctg catgcacgcg cgcgccgcgg tatcggctat ctgccacagg aagcctccat
    47101 tttccgtcgc ctcagcgttt acgataacct gatggcggta ctgcaaattc gtgacgactt
    47161 gtctgctgaa caacgtgaag accgcgcgaa cgagctgatg gaagagtttc acattgagca
    47221 cctgcgtgac agcatggggc agtcactctc cgggggtgaa cgtcgccgtg tagaaattgc
    47281 ccgcgcactg gctgcgaatc cgaaatttat tctgctcgac gaaccgtttg ccggggttga
    47341 cccgatctcg gttatcgata ttaaacgcat cattgaacac ctgcgcgaca gcggcctggg
    47401 cgtgctgatc actgaccaca acgtgcgtga aacactggcg gtttgtgaac gcgcttatat
    47461 cgtcagtcag gggcatttga tcgcccacgg cacgcctaca gaaatcttac aagacgaaca
    47521 cgttaagcgt gtataccttg gggaagactt cagactctga tagggtagaa gtttgcgacg
    47581 ttttagcagg agagtacgat tctgaacatg aagcaaggtt tgcaactcag gcttagccaa
    47641 caactggcga tgacgccaca gctccaacag gcaattcgtc tgttgcagtt gtcgacgctg
    47701 gaacttcagc aggagctaca gcaggcgctg gagagtaatc cgctgcttga gcaaatcgac
    47761 actcatgaag aaatcgacac ccgcgaaacg caagacagtg aaacgctgga caccgccgac
    47821 gcgctcgaac aaaaagagat gccggaagag ctgccgctcg atgccagttg ggacaccatt
    47881 tacaccgctg gtacaccatc cggcaccagc ggtgactaca ttgacgacga gctgccggtc
    47941 taccagggcg aaacgacgca gaccttgcag gattacctga tgtggcaggt cgagctgaca
    48001 ccgttttccg acactgaccg cgctattgct acctctatcg tcgatgccgt tgatgaaacc
    48061 ggttatctga ctgtcccgct ggaagatatt ctcgaaagta tgggcgatga agagatcgac
    48121 atcgacgagg ttgaagccgt ccttaagcgg atccaacggt ttgatccggt cggtgtagcg
    48181 gcaaaagatc tgcgtgactg cctgctaatc caactctccc aattcgataa gaccacgccg
    48241 tggctggaag aggccagatt gatcattagc gatcatctcg atctgttagc caatcacgac
    48301 ttccgcactt taatgcgcgt cacgcgtctg aaagaagatg tgctgaaaga agccgtcaat
    48361 ctgatccagt cgctcgatcc gcgccccggg cagtcgatcc agactggcga acctgagtat
    48421 gtcattccag atgtgctggt gcgtaagcat aacggtcact ggacggtaga actcaacagt
    48481 gacagcattc cgcgtctgca aatcaaccag cactacgcct cgatgtgcaa taacgcgcgc
    48541 aacgatggtg acagccagtt tatccgcagc aatctgcagg atgccaaatg gttgatcaag
    48601 agtctggaaa gccgtaacga tacgctactg cgcgtgagtc gctgtatcgt tgaacagcag
    48661 caagccttct ttgagcaagg tgaagaatat atgaaaccga tggtactggc cgatatcgcc
    48721 caggctgtcg aaatgcatga atcgacgata tctcgcgtga ccacgcaaaa atacctgcat
    48781 agtccacgag gcatttttga actgaagtat ttcttttcca gtcacgtcaa taccgagggc
    48841 ggcggcgaag cttcctccac ggcgattcgt gcactggtga agaaattaat cgcggcggaa
    48901 aacccagcga aaccgttgag cgacagcaag ttaacctctt tgctgtcgga acaaggtatc
    48961 atggtggcac gccgcactgt tgcgaagtac cgagagtctt tatccattcc gccgtcaaac
    49021 cagcgtaaac agctcgtttg acccaaccga taaggaagac actatgcagc tcaacattac
    49081 cggaaataac gtcgagatca ccgaggcact gcgcgaattt gttacagcta aatttgccaa
    49141 acttgagcaa tattttgacc gaatcaacca ggtctatgtt gttctgaaag tggagaaagt
    49201 cacccacacc tcagatgcaa cactgcatgt aaacggtggt gaaattcatg ccagcgcgga
    49261 aggtcaggac atgtacgctg ccattgatgg cttaattgat aagctggcac gccagttgac
    49321 caaacataaa gataaactga aacaacacta attgtccggg caattagcat gtgcatggcg
    49381 gcctgttgtg cggcacaacg ggccatttgt acggttaatg ctccgagcct gttccactgt
    49441 ttgagtgggc aggttcttag gtgaaattat gacaaataat gatacaactc tacagcttag
    49501 cagtgttctt aacagggaat gtacgcgaag ccgcgtccac tgtcagagca aaaaacgcgc
    49561 cctggaaatc atcagcgagc tggcggcgaa acaacttagc ctaccgcctc aggtggtttt
    49621 tgaagctatc ctgacgcggg aaaaaatggg cagtaccggt atcggcaatg gtattgccat
    49681 tccgcatggc aaacttgaag aagatactct gcgtgccgtt ggcgttttcg ttcagctcga
    49741 aacgcctata gctttcgatg ccatcgacaa ccaaccggtg gacttacttt ttgccctgct
    49801 ggtgccggca gaccaaacta aaacgcacct gcatactctg tcgctggtgg cgaaacgtct
    49861 ggcggacaaa accatctgcc gccgtttacg cgcagcccag agcgatgaag agctgtatca
    49921 aatcattacg gataccgaag gtactccgga tgaagcgtag ttattcggta atgtctcttt
    49981 tagacgttgt gaggagaaac agtacatggt actgatgatc gtcagcggac gttcaggttc
    50041 aggtaaatct gtcgccctgc gtgcgctgga agatatgggt ttttactgcg tggataacct
    50101 tcccgtagtg ttgttacccg atctggctcg aactctggcc gatcgcgaga tttctgccgc
    50161 cgtcagcatt gatgttcgta atatgccgga gtcaccagaa atattcgaac aggcgatgag
    50221 taacctgcct gacgctttct caccgcaact actgttcctg gatgccgacc gtaatacctt
    50281 aattcgtcgt tacagtgaca cgcgccgact gcatccgctt tccagcaaaa acctgtcgct
    50341 ggaaagtgct atcgacaaag aaagcgattt gctggagcct ctgcgttcgc gagcggatct
    50401 gattgtcgat acctcagaaa tgtccgttca cgagctggct gaaatgctgc gtacccgtct
    50461 gctgggtaaa cgtgaacgcg aactgaccat ggtctttgag tctttcggct tcaaacacgg
    50521 tatccctatc gatgcagatt acgtctttga cgtgcgcttc ttgccgaacc cgcactggga
    50581 tccgaaactg cgtccaatga caggtcttga taaacctgtc gccgcgttcc tcgaccgcca
    50641 cacagaagta cacaatttta tctaccagac gcgaagctat cttgagctat ggttacctat
    50701 gctggaaacc aacaaccgta gctacctgac ggtcgccatt ggttgtaccg gcgggaagca
    50761 ccgttcggtg tatattgcag agcaactggc agactacttc cgctcgcgcg gtaaaaacgt
    50821 ccagtcacgc catcgtacgc tggaaaaacg taaaccatga ccgtcaagca aactgttgaa
    50881 atcacaaaca agctgggcat gcatgcccgg cctgcaatga agctgtttga attaatgcag
    50941 ggttttgacg ctgaagtgct gttacgtaat gacgaaggca ccgaggctga agccaacagc
    51001 gttattgcgc tgctgatgtt ggattctgcc aaaggacggc agattgaagt tgaagcgacc
    51061 ggtccacagg aagaggaagc actggccgcc gttatcgccc tctttaattc tggttttgat
    51121 gaagattaat cttcatcact ttgacataca actcccttca agctcccccg acaataagaa
    51181 aatcacgtac tgaaatcgtt ctcaatcaac gtcatttgta cattttgtgc gcttttcact
    51241 ttcagaagaa ccttaagaaa accttaagag gcattgttta ggttttgttt aagttaatcg
    51301 accatactgg agatcgtcag aaaatatttc caggagatgg catgattcgc ttatctgaac
    51361 aaagtcccct gggcaccgga agacatcgga aatgttatgc gcatccggaa gatgcccaac
    51421 gctgtatcaa gattgtctac catcgtggcg atggtggcga taaagagatc cgccgtgagt
    51481 taaagtacta cgcgcatctt ggtcgccggt taaaagactg gagtggaata ccgcgctatc
    51541 acggtaccgt tgagacggat tgcggaaccg gttatgtcta cgatgtgatt gccgattttg
    51601 acggcaagcc ttccattacg ctgaccgaat ttgctgaaca atgccgttac gaagaagaca
    51661 ttgcacaact gcgccagcta ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg
    51721 tgacgatgtc gctgaagccg cagaatatcc tctgtcaccg catcagtgag tcagaggtta
    51781 tcccggtcgt ctgcgacaac attggtgaaa gcacattgat tccactggcg acctggtcga
    51841 agtggtgttg tctgcgtaag caagagcggt tatggaaacg atttatcgcc caaccggcgc
    51901 tggctattgc cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg gccctcactt
    51961 cgcgcgaagc ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct aactgataca
    52021 tctgccgtaa aatccacgcc tgacggctac gcacgtagcc gtcaggcgtg gagactttga
    52081 aacgaagtgg attaggtaat acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc
    52141 tcgcgggttt gtggaaataa cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa
    52201 attcggcgat attcaggtaa accgtcagga tacgcttttt gctccagact gtttctatcc
    52261 ccagcgttaa tccagcttcc agcccttttc gtacccagct acgcccatcc cataaaaaga
    52321 gatttttggc cgtctgctga gaaatcgttg aagcaccgcg aatacggttt tcattgcgct
    52381 cgttgtgcgc cagggctttc tcaatggaag cgacatcaaa gccccagtgc tcaggaaatt
    52441 tctgatcttc tgcggcaatc accgccagtc ccatccacgg cgagatttga tccatactga
    52501 cccagtcaga atgtgccacg taacgaaaat tgccatgcag ccaggcgctg acctgtcgct
    52561 cgaccattac cgctgagaag ggaacaggcg caacgctaaa caacgcgatg cccccgcccc
    52621 agaaaacggc gaggacaacc attaaccgca aaagaaaacg gcgaacgaaa ctaaacaccg
    52681 ttaagcggct tttactcatt cagccagaac cagcacgcgg gaaaccagct tatcaatgcc
    52741 gctcgccgct tctgcaatgt tctgcgccag catatatgct ggggtggtga caatcttatt
    52801 gtcttcatca accacgatat catcgacagg acacggcaca tgctccgcgc ccatctcttc
    52861 cagtacttct gcggtatcga tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat
    52921 tttcggcagc atcgccgggg caatacacat aaaaccaagc ggttttccgg cctgatgcat
    52981 cgcttgtgcc agcgccttta attcacggtc aacggtgcat tcgctaccaa gactggcaaa
    53041 attgcttaaa ttcttcgccg cgccaacccc ccccggcaca atcaacgcat ccagttcagc
    53101 ggcatcggcc tgggccagag gacggatttc accgcgcgtt attcgtgccg cttcaatcag
    53161 cacattgcgc gtttccgtca tcgcttcgcc agttaaatgg ttgataacat caacctgctg
    53221 cttatccggt gcaaagcaga ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa
    53281 caccgcttca tgaatttcag aaccgtcata gacgccgcat ccgctcagaa ttacgccaat
    53341 tttcttcatt gtgatcatcc tttcgcaatt gactgaaaca cattaatttt tttaataaaa
    53401 atggtacgca tcacacattt aactgattca tgtaacaaat catttaagtt ttgctatctt
    53461 aactgcgtgc ggcctgaaaa acagtgctgt gcccttgtaa ctcatcataa taatttacgg
    53521 cgcagccaag atttccctgg tgttggcgca gtattcgcgc accccggtct agccggggtc
    53581 attttttagt ggcttttgcc acccacgctt tcagcacttc tacgtcgtga cgccactctt
    53641 ctttcatctc ttcaatccat tcaccgacgt tatcttccca ggcgggaagg tcaggagact
    53701 gaatttgctg acccagctgt tgcagatggc gtaaccccac tgaccccgcc gcacctttaa
    53761 ttttatgtcc ttcctcaaca atgccttttt tatcctgcgc cgtcagattc gactccagca
    53821 cgctgacata gcccggcatc atcttctcaa acaccgctaa cccgtcggtg atcagcttcg
    53881 gtcctacaag ttcgagatac tgttccagca tgggaatatc gagcaatgct tctgatttac
    53941 tgttctcttc tgtcgtcacc gtactcttct catcatcctg ggtatcccag aatttcttga
    54001 tcatcgcggt tagcgccgga accgaaagcg gcttactcag cacatcatcc attccagcat
    54061 tgaggtactc ttgtttgtct ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat
    54121 cctcgcgcgg ataacgcttc gtcagttcac gagagatatc cagcccggtc atatctggca
    54181 actgaatatc cagcaacacc aggtcgtatt cgcccggttt aaacatctcc agcgccgcct
    54241 tgccggtcat ggcgacatca acgctgttac ctaatttttc cagcacagaa cgcgcgacaa
    54301 tcacgttcag ttcaatgtct tccaccagca gcacattcag cgccggtaaa ggcatatcgt
    54361 cttcatcaaa cgcatcatcg acctcttctg ccaccgacgg tgcgtggatc gtcaacgtaa
    54421 aggttgaacc tttgccctgt tcgctggtaa ccgtaatatc gccgcccata tttttcgcca
    54481 gacgacgaga aacggccaga ccaataccgg tgccggtggc aggtttaccg ccatgactgt
    54541 ctttcacctg gtaatacatg gcgaagattt tatccagctc atcctgcgga atgccgatac
    54601 cagaatcttc cacttcaaaa tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa
    54661 cctggccttg ctgggtgaat ttgacggcgt tactgatgag gttccacagg atctgccgta
    54721 aacgcgtccc gtcggtaatg acctgatgcg gtaatggcag cgtaggctcc aggttaaagc
    54781 gcaatccttt ttgttgcgcc tgcaatgcgg agagattttc cagatcggca aggaagctgg
    54841 tgaaatcaac cggctgatta tcaagctgga ccttgcgccg ttccatctta tccatgtcga
    54901 taatatcgtt aaagatattc cccagcgtga cggccgaaac atggatagtt ttgagatatt
    54961 tttcctgctc ggcggtgagt tcggtatcca gcagaatgcg gctcaggccg acgataccgt
    55021 tcagcggtgt acgcaattcg tgactgatgg tggaaataaa cgtcgttttg tcgcggctgg
    55081 cgcgttcaag cgcatcctga taccgcttac gctcggtaat gtcgcgacca aagcccatca
    55141 aaccgtgacg tttacccacg cggtcgtagt acggcacttt acggatttca aagcaggctt
    55201 tgcgcccgtc cgggtaatcc agccactgtt cataggtcag tgacacatta tgacggaaca
    55261 ctttttcatc ggtttcaatg acttttgcgg cggcttccgg tgagtaaaca tcagcaggtt
    55321 tcagatgaac cagttgtttt tcgctttttc cggtcagcag ctccatcgcg cggttacagc
    55381 cggaaaactc tttatcttcg ttacgataaa aaaccaggtc gggtgaagca tcaaggaagg
    55441 aacgtaagaa tgaggattgc tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt
    55501 caattttcag ttggccgaag gtttcctgca gttctgcttc cgctttttca cgaacggcaa
    55561 tttcctgatt tagctgggca atattatctt ttaactgaac gttgaggctc aaatcgcgct
    55621 cgcgcatctc ctccagtttt tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt
    55681 gctcgacgac caccgataga aagtagaccg cccacggcgt aatcagcaaa ccaaagaaga
    55741 tagaacgaat aacatcaatg ctttcgacct gaccatgcag caccatggtt accgccattt
    55801 gtaccacaat ggcaagaacg acgagggcca gcgccagcaa cattgagaag cgcaccagac
    55861 ctaacttcat catcaggtca acataatact gcgccagcag acgaatttgc ttcattaggg
    55921 aattccttca cgacaacctg ccccaataat acccaattct gacagttacg ttataaattg
    55981 tgtgagaaat gcggaattcc cctcaacgac ctactccgtt ggaggtagcc acggacgtcc
    56041 cagcgccgat ccctgtacgc catgttcatt cagatagcga tccagctcga ccattcccgt
    56101 ccatcgattt tcgcaccaca gcggagcaag cagcgtcggg cgacgggcgc tggcggaaat
    56161 gcggtggtag atcacttccg gtggcgtatg gcgaatcatt tctccggcag tgagcgtata
    56221 atcctccagt tcaataccgt tcaaacgccc cgcttcccag gctttcgcca taatgctgcc
    56281 tttcacaata tgcagcggat gcagctttat gccgtccacg ctggtttcaa ccactcgctc
    56341 cagcgtttgc aaacattcag cctgcccttc gccaggcagg ccgacaatta agtgggaaca
    56401 caccttcagc ccgcgctgac gtgccagctg ggttgtacgc tgataacagg caaaatcatg
    56461 gccgcggttg atgcgatgca gtgttttgtc gtgggcggtt tgtagcccca gctccagcca
    56521 cacttcgtaa ccctggtcct tatattcgca aagcagatcc agcaccgcat ccggcacgca
    56581 gtccgggcgg gtaccaacac acaaaccgac aatattggcc tggctcaccg cctgctgata
    56641 catagaacgc agcacctgaa cttccgcaaa ggtgctggta tacgcctgaa agtaggccag
    56701 atagcgttta gcgcggttaa ctaaattcgc ctggtgcgcc agttgctcgg caatggaacg
    56761 atgctgctgc gcttcatcgg caaacgaggc aacattacag aatgtgcagc caccacgccc
    56821 gatggtaccg tcacggttag ggcagctaaa accgccgtgc agcgtcagct tatgcacctt
    56881 ttgcccataa cgacgggtga gatcaccacc aaacatattg actaatttct gtaactgcat
    56941 aatctgatag accgcgcctt gaaaagaggc caaagcctgc catttttagc tcaattcggc
    57001 gatgacctag atcaatcgtc ccgccctgct tttatcaact gcataatcaa tcaaaattac
    57061 cgaaatttca tgcataatca cataaatcac ttttgcttat cttgtgtcag atttttttat
    57121 ctccttatgg attttaggca aaaacagtag catgaaacgt cattaccaat taaggcagta
    57181 taaaatgctg gttttctcgt cagttcaagg caggataagg gttaacacgc ctttatgaca
    57241 gtcaggaatt gactgtttct ctaacgactc tccttttagc cttaaagata aaatctattt
    57301 taatttcagc catttaataa agaattttgc gctaaagcac atttctgtac caataagctt
    57361 gccatttgac ctgtatcagc tttcccgata agttggaaat ccgctggaag ctttctggat
    57421 gagcagcctg ctcatcatat ttatgcagta attgagatcc cctcttcacc gtattaaccg
    57481 atgcgaaaag gacaacaagg gggcgaatgc gaggcgcgcg tatgacacgc aaaccccgtc
    57541 gccacgctct ttctgtgccc gtgcgcagcg gttcggaagt ggggttcccg cagagcctgg
    57601 gggaggttca cgatatgttg tacgataaat cccttgagag ggataactgt ggtttcggcc
    57661 tgatcgccca catagaaggc gaacctagcc acaaggtagt gcgtactgca atacacgcac
    57721 tggcccgcat gcagcaccgt ggcgcgattc tcgccgatgg taaaaccggc gacggttgcg
    57781 gcttgctgtt acaaaaaccg gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc
    57841 gtttagcaaa aaactacgct gtcgggatgc tcttcctgaa taaagatcct gaactcgccg
    57901 ctgccgcacg ccgcatcgtt gaagaagaac tgcaacgcga aaccttgtcg attgtgggct
    57961 ggcgtgatgt ccccactaac gaaggcgtgc tgggtgaaat cgccctctcc tctctgccac
    58021 gcattgagca aatttttgtg aacgccccgg caggctggcg tccacgcgat atggagcgcc
    58081 gtctgtttat cgcccgccgc cgcattgaaa agcgtctcga agccgacaaa gacttctacg
    58141 tctgtagcct gtcgaatctg gtgaacatct ataaaggtct gtgtatgccg gcggatctgc
    58201 cgcgctttta tctggatctt gcggacctgc gtctggaatc ggccatttgc ctgttccacc
    58261 agcgcttctc cactaacacc gtaccgcgct ggccgctggc gcaaccgttc cgctatctgg
    58321 cgcataacgg tgaaatcaac accatcaccg gtaaccgcca atgggcgcgt gcgcgtacct
    58381 ataaattcca gacgccgctg atccctgacc tgcacgacgc cgcgccattc gtcaacgaaa
    58441 ccggttctga ctccagttcc atggataaca tgctggaact gctgctggca ggcgggatgg
    58501 atatcatccg cgccatgcgc ctgttagtgc cacccgcctg gcagaacaac ccggatatgg
    58561 acccggaact gcgtgccttc tttgacttta actccatgca tatggagccg tgggatggtc
    58621 cggcgggcat cgtgatgtcc gatggtcgct tcgctgcctg taacctcgac cgtaacggtc
    58681 tgcgtccggc acgctacgtc attaccaaag acaagctcat cacctgcgcc tctgaagtcg
    58741 gtatctggga ttaccagcct gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac
    58801 tgatggtgat cgacactcgc agtgggcgta ttctgcactc ggcagaaacc gatgacgatc
    58861 tgaaaagccg ccacccttat aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt
    58921 ttgaagatct gcccgatgaa gaagtgggta gccgcgaact ggacgacgac acgcttgcca
    58981 gctaccagaa acagtttaac tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg
    59041 gcgaaaacgg tcaggaagcg gtcggttcga tgggcgatga taccccattc gccgtgctct
    59101 ccagtcagcc gcgcattatt tacgactact tccgccagca gtttgcccag gtgactaacc
    59161 cgccaatcga cccgctgcgt gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg
    59221 aaatgaacgt cttttgcgaa gcagagggcc aggcgcaccg tttaagcttt aaatcgccga
    59281 ttctgctcta ctccgatttc aaacagctca cgacgatgaa agaggagcac taccgcgcag
    59341 atacgctgga tatcaccttt gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc
    59401 tatgcgacaa agccgaaaaa atggtacgta gcggcaccgt gttgctggtg ctctccgacc
    59461 ggaatatcgc taaagatcgc ctgccggttc cggctccgat ggcggttggc gcgatccaga
    59521 cccgtctggt cgatcaaagc ctgcgttgcg atgccaacat catcgtcgaa accgccagcg
    59581 cccgcgatcc gcaccacttc gccgtgttgc tgggtttcgg cgcgacggct atttatccgt
    59641 accttgccta tgaaacgctg ggccgcctgg tagacaccca cgcgattgcc aaagattatc
    59701 gtaccgtgat gctcaactac cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca
    59761 aaatgggcat ctccaccatc gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc
    59821 tacacgatga tgtagtgggc ctgtgcttcc agggcgcggt cagccgcatt ggtggggcaa
    59881 gctttgaaga cttccagcag gatctgctga atctgtcgaa acgtgcctgg ctggcgcgca
    59941 agccgattag ccagggcggc ctgctgaaat acgtccacgg cggcgaatac cacgcctaca
    60001 acccggacgt ggtgcgcacg ctgcaacagg cggtacaaag cggcgagtac agcgactatc
    60061 aggaatacgc gaagctggtt aatgagcgtc cgacaaccac gctgcgcgat ctgctggcaa
    60121 ttacgccggg tgaaaacgcg gtcaacattg ctgatgttga accggcaagc gaactgttta
    60181 aacgctttga taccgccgcg atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc
    60241 tggcggaagc gatgaatagc atcggcggta actcgaactc cggtgaaggc ggcgaagacc
    60301 cggcgcgcta tggcactaac aaagtgtcgc gcatcaagca ggtagcttct ggtcgcttcg
    60361 gcgttactcc ggcgtatctg gtcaatgccg acgtcattca gattaaagtc gcccagggcg
    60421 cgaagccggg cgaaggcggt cagttgccgg gcgacaaagt cactccttac atcgccaaac
    60481 tgcgctattc ggtgcccgga gtgacgctga tctccccgcc gccgcaccac gatatctact
    60541 ctatcgaaga cttagcgcag cttattttcg acctcaagca ggttaacccg aaagcgatga
    60601 tctccgttaa gctggtgtcc gaaccaggcg ttggcaccat cgcgactggc gtggcgaaag
    60661 cttatgcgga cttgatcacc atcgcgggct atgacggcgg caccggcgca agcccgctgt
    60721 cgtcagtgaa atatgcaggc tgcccgtggg aactcggtct ggtggaaacc caacaggcgc
    60781 tggttgccaa cggcttgcgt cataaaattc gtttgcaggt tgatggcggc ctgaaaacgg
    60841 gtgtcgatat catcaaagcg gcgattctcg gcgcagaaag cttcggcttc ggcactggcc
    60901 caatggtggc gctcggctgt aaatatctgc gtatttgcca cctgaacaac tgcgccacgg
    60961 gtgtagcaac tcaggatgac aaactgcgta agaatcacta tcacggcctg ccgttcaagg
    61021 tgactaatta ctttgagttt atcgcccgtg aaacccgcga gctgatggca cagctgggag
    61081 taacgcgtct ggtggatctg attggtcgca ccgacctgct gaaagagctg gacggtttca
    61141 ccgccaaaca gcagaaactg gcgctgtcga agctgctgga gactgccgag ccacatgcag
    61201 gtaagtcact ctactgcact gaaaacaacc cgccgtttga taacggccta ctgaacgcgc
    61261 agttgctgca acaggcgaaa ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg
    61321 atatccgcaa caccgaccgt tctgtcggcg cgtcgctttc aggctatatc gcccagacgc
    61381 acggcgatca ggggctggca gccgatccta tcaaagcgta cttcaacggc accgcaggcc
    61441 agagtttcgg tgtgtggaac gcgggcggcg tggaactata cctgaccggg gatgccaacg
    61501 actatgtcgg taaaggcatg gcgggcggct taatcgccat tcatcctccg gttggttccg
    61561 ccttccgcag ccatgaagca agcattatcg gcaacacctg cctgtatggc gcgaccggtg
    61621 gtcgtctgta tgccgcaggc cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta
    61681 tcaccgtggt agaaggcatt ggcgacaacg gctgtgaata tatgacgggc ggtattgtct
    61741 gtattctggg taaaaccggc gttaacttcg gcgcgggcat gaccggcggc ttcgcttacg
    61801 ttctcgatga aagcggtgat ttccgcaaac gcgttaaccc ggaactggtc gaagtcttaa
    61861 gcgtcgacga tctggcgatc catgaagagc atctgcgcgg tcttatcacc gagcatgttc
    61921 agcataccgg ttctcagcgc ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacga
    61981 aattcgcgct ggttaaaccg aagtccagtg atgtaaaagc actgctgggt caccgtagtc
    62041 gtagcgcagc agagttgcgc gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg
    62101 tttatcaatt tatcgacctg cagcgcgttg atccgccaaa gaaaccgctg aagatccgta
    62161 aaattgagtt tgttgaaatt tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg
    62221 accgctgtct gtcgtgcggc aacccatact gcgagtggaa atgcccggta cacaactaca
    62281 tcccaaactg gctgaagctc gccaacgagg ggcgtatttt tgaagcggca gaactgtcgc
    62341 atcagaccaa cacactgccg gaagtatgcg gacgtgtctg cccgcaagac cgtctgtgcg
    62401 aaggttcctg caccctgaac gatgagttcg gtgcggtgac catcggcaac attgagcgtt
    62461 atatcaatga taaagcgttc gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa
    62521 ccggtaaaaa agtggcgatt atcggtgcag gcccggcagg tctggcgtgt gcggatgtcc
    62581 tgacgcgtaa cggcgtaaaa gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc
    62641 tgaccttcgg tattccggcc ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa
    62701 tcttcaccgg catgggtatt gaattcaaac tcaataccga agtgggccgc gacgtacagc
    62761 tggacgatct gctgagtgat tacgatgccg tgttccttgg cgttgggact tatcagtcaa
    62821 tgcgcggcgg gctggaaaac gaagacgccg atggcgtgta cgcagcgctg ccgttcctta
    62881 tcgccaacac caaacagtta atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca
    62941 tggaaggcaa acgcgtggta gtacttggcg gtggcgacac tgcgatggac tgcgtgcgca
    63001 cgtctgtacg ccagggggca aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca
    63061 tgccgggttc ccgccgcgaa gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca
    63121 acgtccagcc actggggatt gaagtgaacg gtaacggcaa agtcagcggc gtaaaaatgg
    63181 tacgtaccga aatgggcgaa ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag
    63241 gttccgaaca tatcgttccg gcagatgcgg tgatcatggc gtttggtttc cgtccacaca
    63301 acatggaatg gctggcaaaa cacagcgtcg agctggattc acaaggccgc atcatcgctc
    63361 cggaaggcaa cgacaacgct ttccagacca gcaacccgaa aatctttgct ggcggcgata
    63421 tcgtccgtgg ttccgatctg gtcgttaccg ccatcgccga aggtcgcaaa gcggcagacg
    63481 gcattatgaa ctggctggaa gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt
    63541 cggcgctgaa attctccttt ctttctggaa atcgcctcgc ataatctgaa gaaaaccaat
    63601 gtaatacgct catctggatt gttaccggta gatgcccggc gtatagctga ttccattatt
    63661 caacgcagcg ccatataaag ggacagataa tggaatcgct ctcagagaga acctcaacag
    63721 gctaccagca aatccacgac ggcattattc atctggtcga tagcgcccga acggaaacgg
    63781 tacgtagcgt taacgcgctg atgaccgcga cgtactggga aattggccga cggattgtcg
    63841 aatttgaaca aggtggtgag gccagagcag cttatggtgc acagttgatt gatcggctat
    63901 cgaaggattt aagtcgacgg tataaacgtg gggtctctgc aaaaaactta cgccaaatga
    63961 gg
//
LOCUS       DAJVQH010000010        60720 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJVQH010000010 DAJVQH010000000
VERSION     DAJVQH010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.010"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..367
                     /locus_tag="OQM87_000728"
     CDS             <1..367
                     /locus_tag="OQM87_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1506975.1"
                     /translation="SPSYTKSVSWQHYPTKVGTENGQLLGNTLTGNDAAKGVGVLIEG
                     LATSKNPLMTLKPNDSNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGT
                     IPIEAGEFKATSTFQVTYP"
     gene            480..1274
                     /gene="panB"
                     /locus_tag="OQM87_000729"
     CDS             480..1274
                     /gene="panB"
                     /locus_tag="OQM87_000729"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCS1506976.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1286..2137
                     /gene="panC"
                     /locus_tag="OQM87_000730"
     CDS             1286..2137
                     /gene="panC"
                     /locus_tag="OQM87_000730"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCS1506977.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2211..3113)
                     /gene="rpnC"
                     /locus_tag="OQM87_000731"
     CDS             complement(2211..3113)
                     /gene="rpnC"
                     /locus_tag="OQM87_000731"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCS1506978.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3387..3767
                     /gene="panD"
                     /locus_tag="OQM87_000732"
     CDS             3387..3767
                     /gene="panD"
                     /locus_tag="OQM87_000732"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCS1506979.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3771..5000)
                     /locus_tag="OQM87_000733"
     CDS             complement(3771..5000)
                     /locus_tag="OQM87_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1506980.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5064..5504)
                     /locus_tag="OQM87_000734"
     CDS             complement(5064..5504)
                     /locus_tag="OQM87_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1506981.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5609..6379)
                     /locus_tag="OQM87_000735"
     CDS             complement(5609..6379)
                     /locus_tag="OQM87_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1506982.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6376..7302)
                     /locus_tag="OQM87_000736"
     CDS             complement(6376..7302)
                     /locus_tag="OQM87_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1506983.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7411..8073
                     /gene="can"
                     /locus_tag="OQM87_000737"
     CDS             7411..8073
                     /gene="can"
                     /locus_tag="OQM87_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCS1506984.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8114..8650)
                     /gene="hpt"
                     /locus_tag="OQM87_000738"
     CDS             complement(8114..8650)
                     /gene="hpt"
                     /locus_tag="OQM87_000738"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCS1506985.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8856..11246
                     /gene="gcd"
                     /locus_tag="OQM87_000739"
     CDS             8856..11246
                     /gene="gcd"
                     /locus_tag="OQM87_000739"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCS1506986.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHAFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11293..12842)
                     /gene="cueO"
                     /locus_tag="OQM87_000740"
                     /pseudo
     CDS             complement(11293..12842)
                     /gene="cueO"
                     /locus_tag="OQM87_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            13008..13355
                     /locus_tag="OQM87_000741"
     CDS             13008..13355
                     /locus_tag="OQM87_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCS1506987.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13461..14327
                     /gene="speE"
                     /locus_tag="OQM87_000742"
     CDS             13461..14327
                     /gene="speE"
                     /locus_tag="OQM87_000742"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCS1506988.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14343..15137
                     /gene="speD"
                     /locus_tag="OQM87_000743"
     CDS             14343..15137
                     /gene="speD"
                     /locus_tag="OQM87_000743"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCS1506989.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15175..15537)
                     /locus_tag="OQM87_000744"
     CDS             complement(15175..15537)
                     /locus_tag="OQM87_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCS1506990.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15713..18310)
                     /gene="acnB"
                     /locus_tag="OQM87_000745"
     CDS             complement(15713..18310)
                     /gene="acnB"
                     /locus_tag="OQM87_000745"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCS1506991.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18665..20518
                     /locus_tag="OQM87_000746"
     CDS             18665..20518
                     /locus_tag="OQM87_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCS1506992.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNSITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQATASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(20589..22019)
                     /gene="lpdA"
                     /locus_tag="OQM87_000747"
     CDS             complement(20589..22019)
                     /gene="lpdA"
                     /locus_tag="OQM87_000747"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCS1506993.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPKAKKK"
     gene            complement(22344..24236)
                     /gene="aceF"
                     /locus_tag="OQM87_000748"
     CDS             complement(22344..24236)
                     /gene="aceF"
                     /locus_tag="OQM87_000748"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCS1506994.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPVAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24251..26914)
                     /gene="aceE"
                     /locus_tag="OQM87_000749"
     CDS             complement(24251..26914)
                     /gene="aceE"
                     /locus_tag="OQM87_000749"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCS1506995.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27075..27839)
                     /gene="pdhR"
                     /locus_tag="OQM87_000750"
     CDS             complement(27075..27839)
                     /gene="pdhR"
                     /locus_tag="OQM87_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCS1506996.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28380..29753
                     /gene="aroP"
                     /locus_tag="OQM87_000751"
     CDS             28380..29753
                     /gene="aroP"
                     /locus_tag="OQM87_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCS1506997.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29796..30650)
                     /gene="ampE"
                     /locus_tag="OQM87_000752"
     CDS             complement(29796..30650)
                     /gene="ampE"
                     /locus_tag="OQM87_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCS1506998.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30647..31198)
                     /gene="ampD"
                     /locus_tag="OQM87_000753"
     CDS             complement(30647..31198)
                     /gene="ampD"
                     /locus_tag="OQM87_000753"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCS1506999.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31286..32179
                     /gene="nadC"
                     /locus_tag="OQM87_000754"
     CDS             31286..32179
                     /gene="nadC"
                     /locus_tag="OQM87_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCS1507000.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNDKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32382..32822
                     /gene="ppdD"
                     /locus_tag="OQM87_000755"
     CDS             32382..32822
                     /gene="ppdD"
                     /locus_tag="OQM87_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCS1507001.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32832..34217
                     /gene="gspE"
                     /locus_tag="OQM87_000756"
     CDS             32832..34217
                     /gene="gspE"
                     /locus_tag="OQM87_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HCS1507002.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAVQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34207..35409
                     /gene="hofC"
                     /locus_tag="OQM87_000757"
     CDS             34207..35409
                     /gene="hofC"
                     /locus_tag="OQM87_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCS1507003.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35444..36487)
                     /gene="guaC"
                     /locus_tag="OQM87_000758"
     CDS             complement(35444..36487)
                     /gene="guaC"
                     /locus_tag="OQM87_000758"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCS1507004.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36649..36687)
                     /locus_tag="OQM87_000759"
     CDS             complement(36649..36687)
                     /locus_tag="OQM87_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507005.1"
                     /translation="MIIKTLKMSARK"
     gene            36712..37332
                     /gene="coaE"
                     /locus_tag="OQM87_000760"
     CDS             36712..37332
                     /gene="coaE"
                     /locus_tag="OQM87_000760"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCS1507006.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLIENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37332..38075
                     /gene="zapD"
                     /locus_tag="OQM87_000761"
     CDS             37332..38075
                     /gene="zapD"
                     /locus_tag="OQM87_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCS1507007.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38085..38282
                     /gene="yacG"
                     /locus_tag="OQM87_000762"
     CDS             38085..38282
                     /gene="yacG"
                     /locus_tag="OQM87_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCS1507008.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38382..38771)
                     /gene="mutT"
                     /locus_tag="OQM87_000763"
     CDS             complement(38382..38771)
                     /gene="mutT"
                     /locus_tag="OQM87_000763"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCS1507009.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38831..41536)
                     /gene="secA"
                     /locus_tag="OQM87_000764"
     CDS             complement(38831..41536)
                     /gene="secA"
                     /locus_tag="OQM87_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCS1507010.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKLGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41598..42110)
                     /gene="secM"
                     /locus_tag="OQM87_000765"
     CDS             complement(41598..42110)
                     /gene="secM"
                     /locus_tag="OQM87_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCS1507011.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42341..43258)
                     /gene="lpxC"
                     /locus_tag="OQM87_000766"
     CDS             complement(42341..43258)
                     /gene="lpxC"
                     /locus_tag="OQM87_000766"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCS1507012.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43359..44510)
                     /gene="ftsZ"
                     /locus_tag="OQM87_000767"
     CDS             complement(43359..44510)
                     /gene="ftsZ"
                     /locus_tag="OQM87_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCS1507013.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44571..45833)
                     /gene="ftsA"
                     /locus_tag="OQM87_000768"
     CDS             complement(44571..45833)
                     /gene="ftsA"
                     /locus_tag="OQM87_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCS1507014.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45830..46660)
                     /gene="ftsQ"
                     /locus_tag="OQM87_000769"
     CDS             complement(45830..46660)
                     /gene="ftsQ"
                     /locus_tag="OQM87_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCS1507015.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46662..47582)
                     /gene="ddlB"
                     /locus_tag="OQM87_000770"
     CDS             complement(46662..47582)
                     /gene="ddlB"
                     /locus_tag="OQM87_000770"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1507016.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47575..49050)
                     /gene="murC"
                     /locus_tag="OQM87_000771"
     CDS             complement(47575..49050)
                     /gene="murC"
                     /locus_tag="OQM87_000771"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCS1507017.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49104..50171)
                     /gene="murG"
                     /locus_tag="OQM87_000772"
     CDS             complement(49104..50171)
                     /gene="murG"
                     /locus_tag="OQM87_000772"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCS1507018.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNGWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50168..51412)
                     /gene="ftsW"
                     /locus_tag="OQM87_000773"
     CDS             complement(50168..51412)
                     /gene="ftsW"
                     /locus_tag="OQM87_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCS1507019.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51412..52728)
                     /gene="murD"
                     /locus_tag="OQM87_000774"
     CDS             complement(51412..52728)
                     /gene="murD"
                     /locus_tag="OQM87_000774"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCS1507020.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52731..53813)
                     /gene="mraY"
                     /locus_tag="OQM87_000775"
     CDS             complement(52731..53813)
                     /gene="mraY"
                     /locus_tag="OQM87_000775"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCS1507021.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53807..55165)
                     /gene="murF"
                     /locus_tag="OQM87_000776"
     CDS             complement(53807..55165)
                     /gene="murF"
                     /locus_tag="OQM87_000776"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCS1507022.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIARTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEDCHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55162..56649)
                     /gene="murE"
                     /locus_tag="OQM87_000777"
     CDS             complement(55162..56649)
                     /gene="murE"
                     /locus_tag="OQM87_000777"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCS1507023.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56636..58402)
                     /gene="ftsI"
                     /locus_tag="OQM87_000778"
     CDS             complement(56636..58402)
                     /gene="ftsI"
                     /locus_tag="OQM87_000778"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCS1507024.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58418..58783)
                     /gene="ftsL"
                     /locus_tag="OQM87_000779"
     CDS             complement(58418..58783)
                     /gene="ftsL"
                     /locus_tag="OQM87_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCS1507025.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58780..59721)
                     /gene="rsmH"
                     /locus_tag="OQM87_000780"
     CDS             complement(58780..59721)
                     /gene="rsmH"
                     /locus_tag="OQM87_000780"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCS1507026.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59723..60181)
                     /gene="mraZ"
                     /locus_tag="OQM87_000781"
     CDS             complement(59723..60181)
                     /gene="mraZ"
                     /locus_tag="OQM87_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCS1507027.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14553 a        16812 c        14923 g        14432 t
ORIGIN      
        1 atcaccatca tacactaaat cagtaagttg gcagcattac ccaacaaagg ttggtactga
       61 aaacgggcaa ctccttggta atactctcac aggtaatgac gcagcaaaag gagtcggtgt
      121 actcatagaa ggtttagcaa ctagtaaaaa tcctctaatg acattgaaac ctaatgattc
      181 aaattctgtt tataaagatt acgacccgag aggcaaagac gatacaacag gaggagttta
      241 cccggatcaa gatacaggta taacataccc tctccatttc caggccacgc tacaacagga
      301 tggaactata ccaatagaag ctggtgagtt taaagccacc agtactttcc aggtaaccta
      361 cccttaataa gtccaccgca ccgccatcat ctggcggtgc ggtaattgat aaatcattgc
      421 ccccaccaat gtgactgata aaatgccagg caacacgaca tcatttatca ggatacgtta
      481 tgaaaccgac caccattgct tcacttcaga aatataagca ggataaaaag cgtttcgcga
      541 ccatcaccgc ttacgactac agcttcgcga aactatttgc tgatgaaggg cttaacgtca
      601 tgctggtagg cgactcgctg ggcatgaccg ttcagggcca cgattccact ctgccagtaa
      661 ccgttgctga tatcgcctac cacactgccg ccgtacgtcg cggcgcacca aactgcctgc
      721 tattggctga cctgccgttt atggcgtatg ccacgccgga acaagccttt gaaaacgccg
      781 caacggttat gcgtgccggt gccaatatgg tcaaaattga gggcggtgag tggctggtcg
      841 aaaccgtaaa aatgctgacc gaacgtgccg ttcctgtatg tggtcactta ggtttaaccc
      901 cacagtcagt gaatattttc ggtggctaca aagtacaggg gcgcggcaat gaagcgagcg
      961 atcgactact cagcgatgca ttagccttag aagccgcggg ggcacagctg ctggtgctgg
     1021 aatgcgtgcc ggttgagctg gcaaaacgta ttaccgaagc actggcgatc ccggttattg
     1081 gtattggtgc aggcaacgtc actgacgggc aaatcctcgt gatgcacgac gcctttggta
     1141 ttaccggcgg tcacattcct aaatttgcta aaaatttcct cgccgaaacg ggcgacatcc
     1201 gcgcggctgt gcggcagtat atggctgaag tggagtccgg cgtttatccg ggcgaagaac
     1261 acagtttcca ttaaggagtc acgttgtgtt aattatcgaa accctgccgc tgctgcgtca
     1321 gcaaattcgc cgcctgcgta tggaaggcaa gcgcgtggcg ctggtgccta ctatgggtaa
     1381 tctgcacaat ggccatatga agctggtcga cgaagccaaa gcccgcgccg atgtggtcgt
     1441 cgtcagtatt ttcgttaacc cgatgcagtt cgaccgcccg gaagatctgg cacgttatcc
     1501 acggaccttg caggaggact gcgagaagct aaacaaacgt aaagtggatt tagttttcgc
     1561 cccttcggta aaagagatct acccgaacgg tactgaaacc cacacttacg ttgacgttcc
     1621 tggcctttca accatgctgg aaggtgccag ccgtccggga cattttcgcg gcgtttcgac
     1681 tattgtcagc acgctgttca acctggtcca gccggacatc gcctgcttcg gtgaaaaaga
     1741 ttttcagcaa ctggcgttga tccgcaaaat ggttgccgat atgggcttcg atattgagat
     1801 tgtcggtgtg ccaattatgc gcgccaaaga cggtctggcg ctaagttccc gtaacggtta
     1861 tctgacggcg gaacaacgca aaattgcgcc tggtctgtac aaagttttaa gttcgattgc
     1921 tgacaaattg caggctgggg aacgggatct cgatgaaatt attaccattg cggggcaaga
     1981 actgaatgaa aaaggcttcc gcgccgatga tattcagatt cgcgatgccg acacattgct
     2041 ggaagtttct gaaaccagca aacgggcagt aattctggta gccgcctggc ttggcgatgc
     2101 tcgcctgatc gacaacaaaa tggtcgagct ggcgtaatac ttaactggcg ctacggctga
     2161 tggcgccagt tattaattta ccccacgtca tctgtcataa cacaggtaac ttaaataagg
     2221 ttaattacct tatcaatctc agcaagaggt aaatttgcca tctctgcaac gtcttcccga
     2281 gacattcctt tacttagaag acgcagggcg aattcttgcc tttcctcctg tcttccctgc
     2341 ggaatcccct tctcaatccc tttctcttca aaccactgcg ccagcgtcat catagacttc
     2401 cctcccgttt ccctgtctct cagcacacca taaaacaaat ccgcttgttc agtatgaccg
     2461 cgttgcagca tatagttttg catggcaact aactgacttc cgctagtgta cccttcgtca
     2521 atcagcgtga ccagttgctc cagcaataac attaagtcgc gctggcgaat atgtttttgc
     2581 agtagttcga gaatcgcaat ccgccgatgt tgcatgattt cgtcatccgg tgtgatggtg
     2641 atatccacca gcgggaaagg actgttatag acgcgtcgcg ccagctccgg cgagtaaaac
     2701 atatcaaacc agcacattga gagcggataa ggtgtggcct cgccctgata aaacagtatc
     2761 ggcaccacca gcggcagctt atcgtggcca gcctccagat gccggtgcat ggcagcaata
     2821 gaataacgca tcatgcgaaa ggccattttc ttatccggct tgctttggtg ttcaatcaca
     2881 acatgcagat aaccgggatt gccctgcatt tgcacggaat agagcacgtc cgtgctgtga
     2941 cctttcaggc tctcttcaat gaaactcccc gactctaaat gaagcgtgtt gagatcacaa
     3001 agttcgcgta attccactgg caaatgtatc tccagaaagt cgcgagccgt ctccgcatgc
     3061 attaaaaatt gtttaaatac cgcgtcatgc ggtgtggtac tcggtgcatc catcttccct
     3121 gtcttccgtg tggccttgcg tgaaggcagt atccagtcaa ggcgtgctat cgtcagccac
     3181 ctctttattg tttaccgagc agcattcata aaaatatcaa ttcgttacag gcgttacatg
     3241 gcacgcttcg gcgcggtacg caaactgctg acgcaaatgc catcagatga gtagacactc
     3301 atcaaaaatc gggcaatact gcgtgagaat ttcctcaaag caggcaccgc ctgcttcgtt
     3361 aacgacaggg tagaaaggta gaagttatga ttcgcacgat gctgcagggc aaactccacc
     3421 gcgtgaaagt gactcatgcg gacctgcact atgaaggttc ttgcgccatt gaccaggatt
     3481 ttcttgacgc agccggtatt ctcgaaaacg aagccatcga tatctggaat gtcaccaacg
     3541 gcaagcgttt ctccacttat gccatcgcgg cagaacgcgg ttcgagaatt atttctgtta
     3601 acggtgcggc ggcccactgc gccagcgtcg gcgatattgt catcatcgcc agcttcgtta
     3661 ccatgccaga tgaagaagct cgcacctggc gacccaatgt cgcctatttt gaaggcgaca
     3721 atgaaatgaa acgtaccgcg aaagcgattc cggtacaggt tgcttgattg ttatccctgc
     3781 ggctggttac tcaccagccg cgacatcttc tccagcgaat ccgttcttaa gatataaagt
     3841 cgttttagta acaacggatt atcccccggt tttactttgc ctttcatggt tgtcaccgcc
     3901 aggtgaaatc cggcatcgtt tgctgccttc acggctttgt cattaaagcc gccaaacgga
     3961 tacgaaagat accagacatg tggattaaat tgcgccagag cgcggcgtga acgtgcaaaa
     4021 tcaaacagaa tattgtgctc actacggctc agtaatatgg gtcggcgata accatctacc
     4081 cgatgcaaaa aatgggtatg tgactggaaa tcaaaaacat cgcgaatttc gttaagctca
     4141 gaaacgctca taaattgcag cgattttggg ttccacttct gcgggtgacg tttgatgcgt
     4201 gaggtaacaa taaacaccgt cgccttcatg ccatattgtt tcaacacagg atacgcatag
     4261 cggctcaccg acttgaggcc atcatcaaag gtaatcacca ccgctcgcgc agggagattg
     4321 atcgtattct tcacgtaacc ttccagctga gccatgctca gtgtcgcgta tcccctgtcg
     4381 cgcagccagg ccatttggtt attgaaagcg cgtaccgagg tggtcgtcga agtatggcga
     4441 aaacgggtgt tttcttcgtc gcgcagaata tggtgataag ttagcaccgg caggccatta
     4501 tcgggttggg catccagcgc gctgatatag gccagtcgat cgccaatacg gatctgatac
     4561 caggtttgat ttaacctgtc tttcagttta tgcaaaatcg ggtagcgcaa attgtccgcc
     4621 agtaccccaa atggcgcact gcccacactc ggcgcgttgt agaccggcgt atctttccag
     4681 gtaattaagt tctgattact cagcggcttg ttaaggtcac ccaaaccgtc ttcaactttt
     4741 tgtcgcccct gaactggctc gagatgccct ttatcgataa accctttacc aaagccaaaa
     4801 ttaaatgcgt aataacttgc ggcagtgggc tctacggcaa tgatttgtcc ggcccgaata
     4861 ttccccacgg tcaccatctt gtcaccaatt tgcgcccaga ccgcagcatt ttcgatggtt
     4921 tgcatataac gggcaggtaa cgcggcactg acacttgcgg taaacagcat cagcaggaga
     4981 ataacagctt gtttatacat gaaaaaataa ccgagggtga aacaacgctt ttattgtagc
     5041 aaaagcgcca tcagaatcgc gggttaatac tcatacaaat tatgtttgag gacgaaaggc
     5101 ggattttttt gttgttggtg ccaaagacta ctcacctccg gcccacccag ttcgacaata
     5161 cactcacgaa actctgaact ggtcatggtt tcacggcaag ccatcatctg ttcaattaac
     5221 ggtaacgtga caccagaaat cacttcgcaa cgggagtgtt tgtggcttaa taatgaagcc
     5281 acgcgatacg gtggcgctcc ggctatatcg gttaagaaga tgacaccctc accagagtcc
     5341 gcttcatgca gagcatcgca catcatgcgg ctgagcatat tagagcttaa tccgcgccag
     5401 aaattcacgg cccggcactg aagaagtgcc ccatgttttt tctccagcgc atccagtatc
     5461 tcttgcgccc ggtcatcgtg acaggtaatt acccaaccta acatcgcctt cctccttagc
     5521 cgaaaatcag cctaccctga ccattgttga taaagggtga tagccgtcaa aactcctctc
     5581 ccccgaatcc agaggagagg aaatagcctt agctacgcaa accacgtcca cgttggatca
     5641 gcgaccaaca gatcaaataa aacgccacaa taaagaccac cagtacgcca aaggtagtga
     5701 ccagcggcac atcatttata ccgaggaagc cgtagcggaa accactgatc atataaacga
     5761 ttgggttcag gtgcgacagc ccttgccaga acggcggcaa caaagtcagt gagtaaaaga
     5821 ccccgcccaa atacgtgagt ggcgttaaca caaaggttgg caccaggctg atgtcatcga
     5881 acgttttggc aaacacgccg ttcagcaaac ccgcaaggga gaatagcacc gccgtgagca
     5941 ccagggttaa ggcaacgaat acccacgaat gcacctgaaa cggcacaaaa aacagtgaaa
     6001 ttgccgtcac cagaatgcca acaaacagac cacgcgccac gccaccgccg acatatccgg
     6061 caataatgac gtgagtcgga accggcgcta ccagcagctc ttcaatatta cgctggaact
     6121 tggcaccaaa aaatgatgac gcgacgttag cgtaggcgtt ggtgatcacc gacatcatga
     6181 tcagccccgg tacgatgaac tgcatatagc tgaagccatg catatcgcca atacgcgaac
     6241 ctatcaggtt accgaagata ataaagtaaa gggtcatggt gatgactggc ggcaccagcg
     6301 tctgcaccca gatacgcata aagcgatgga tctctttcgc ccagatgctt tttagcgcca
     6361 cccagtaaag atgcatcatg cgcgatctcc ttgtttttca ttaaccagtg aaacaaacag
     6421 ctcttccaga cggttagctt tgttacgcat acttaatacc tgaatgccct gctcacttaa
     6481 ctgcgtaaat acgctgttga tcccctgctc acgtagcact tcaacttcca gcgtcgcagt
     6541 atcgaccagt cgatactgat agccatcgag cttcggtaac ggacttttcg gtgcgagatc
     6601 gagaataaag gtttccgatt tcagcttcgc cagcagcgcc ttcatcgagg tattttccac
     6661 cagctcacca tgttgaataa tgccgatatt gagacacagc atttctgctt cttccaggta
     6721 gtgtgtggta agaatgatgg tggtgccttt gtcgtttaaa tccttcaaaa agccccacat
     6781 tgagcggcga agttcaatat ccacgcctgc ggtcggttcg tcgagaatca gtagtttagg
     6841 ttcatgcatt aacgcacggg caatcattaa acggcgcttc atcccgccag ataacatacg
     6901 cgcacgttcg ttgcgttttc cccataaatc gagctgctta agatattttt cgctgcggat
     6961 gtacgcctct ttgcgctcca caccgtagta ccctgcctga ttcaccacaa tttgctgcac
     7021 ggtttcaaac gggttgaagt taaattcctg cggcaccagt cccaactgac gtttagcgtt
     7081 cacgacatct ttctcgagat cgtaaccaaa tacgctgacc cgcccggagg ttttatttac
     7141 cagagagctg ataataccga tagtggtcga tttcccggcc ccgttcggcc cgagaagcgc
     7201 ataaaaatca cccgcttcga cctgcaaatc tattccacga agcgcctgaa cgccgcctgg
     7261 atagtttttt ttaagctgtt gaagttccag tgcaatggtc ataaattttt acttacctta
     7321 cgttcttaca ctttatctgt ggtttaaatc gtcccggagt tgccctatat tagccaaacg
     7381 taattatttg gttacaggtc gttaacctcc atgaaagaca tagatacact catcagcaac
     7441 aatgcactat ggtcaaaaat gctggtggaa gaggatcccg ggttttttga gaaactggca
     7501 caagcgcaaa aaccgcgctt tctatggatt ggatgttccg acagtcgcgt tcctgcagaa
     7561 cgtttaaccg gtcttgagcc gggcgaactc tttgttcacc gtaatgttgc taacctggtc
     7621 attcacaccg acctgaactg cctttccgtg gttcagtatg cagtggatgt actcgaagtt
     7681 gaacacatta ttatctgtgg ccactacggt tgcggcggcg tacaagccgc cgttgaaaac
     7741 ccggaactgg ggcttatcaa caactggctg ctgcatatcc gcgatatctg gttcaaacat
     7801 agctcattgc tcggcgaaat gccgcaagag cgccgtctgg ataccttgtg tgaactgaac
     7861 gtcatggaac aggtgtataa cctgggccac tccaccatta tgcaatcagc gtggaaacgc
     7921 gggcagaaag ttaccattca cggctgggcc tacggcattc acgacggctt gctgcgtgat
     7981 ctggatgtta ccgccaccaa ccgcgaaacc cttgagcaac gttaccgtca cgggatttcc
     8041 aacctcaagc tgaaacacgc caaccacaaa taaaaatgcc atgccggatg caacacatcc
     8101 ggcaacttca cacttactcg tccagcagaa tcactttgcc aatatacggc agatgacggt
     8161 aacgctgtgc gtaatcaatg ccgtaaccca ccacaaactc atccgggatc gagaaaccga
     8221 taaattcaac cgggacgttc acttcacgac gggacggttt atccagcagc gtacaaatcg
     8281 ccaacgactt cggttcgcgc aggcttaaga tctcacgcac tttcgacagt gtattccccg
     8341 agtcgatgat atcttcaaca atcagcacgt ccttgccacg gatatcttca tccagatctt
     8401 tgaggatttt cacatcacgg gtggtggaca tgccgctacc gtagctggag gcggtcataa
     8461 agtcgacttc atgagatacc tgaacttcac ggcacaggtc cgccataaac ataaatgagc
     8521 cacgcagcag acccaccagc accatatcgc tgccgctgtc tttgtaacgc tcagtaatct
     8581 gacgacccag ttcggcgata cgcgctttaa tctccgcttc ggggatcatt acttctacag
     8641 tatgtttcat atctctaacc atatgattta aaaacaaatc acttcgcacg ggcaggtttt
     8701 ggcaccgcaa caaagcgcaa gacacgcagt ataccagtaa aactattcat tcgcggaatc
     8761 tgttaataaa gctcgaattg tgatgacggt cacacatgtt aaaatctata attaattgct
     8821 actaaaatat taatgaattg aaatggtgtc tctttatggc aattaacaat acaggctcgc
     8881 gacgattact cgtcacgcta acagcccttt ttgcagcgct ttgcgggctg tatctactca
     8941 ttggcggagg ctggctggtc gcgattggcg gctcctggta ctaccctatc gctggccttg
     9001 tgatgctcgg cgtcgcctgg atgctgtggc gcagtaaacg cgccgcgctt tggctatacg
     9061 cagccctgct gctcggcacc atgatttggg gcgtctggga agttggtttc gacttctggg
     9121 cgctgactcc gcgcagcgat attctggtct tcttcggcat ctggctgatc ctgccgtttg
     9181 tctggcgtcg cctggtcatt cctgccagcg gcgcagttgc cgcactggtg gtcgcactgc
     9241 tgattagcgg tggtatcctg acctgggccg gatttaacga tccgcaggag atcaacggca
     9301 ccttaagcgc cgatgccaca cctgccgaag ctatctcccc cgttgccgat caggactggc
     9361 ctgcgtatgg ccgcaatcag gaaggtcaac gcttttcacc actgaagcaa attcacgccg
     9421 ataacgtcca caagctgaaa gaagcctggg tgttccgtac tggcgatgtg aagcagccga
     9481 acgatccggg tgaaatcacc aatgaagtga cgccgattaa agtgggcgat accctttacc
     9541 tgtgtaccgc tcaccagcgc ctgtttgcgc ttgatgccgc cagcggcaaa gagaaatggc
     9601 attacgatcc tgagctgaaa accaacgagt ctttccagca cgtaacctgc cgtggcgtct
     9661 cttatcatga agccaaagca gaaacggctt cgccggaagt gatggcggat tgcccgcgtc
     9721 gtatcattct tccagtgaat gatggtcgcc tgattgcgat taacgctgaa aacggcaaac
     9781 tgtgcgaaac cttcgccaat aaaggcgtgc tcaatctgca aagcaatatg ccagacacca
     9841 aaccgggtct gtatgagccg acttctccgc caattatcac cgataaaacc atcgtgatgg
     9901 ccggttcagt caccgataac ttctcaaccc gcgaaacgtc tggcgtgatc cgtggttttg
     9961 atgtcaacac cggggagctg ctgtgggctt ttgatccggg cgcgaaagat ccgaacgcaa
    10021 tcccgtctga cgaacacgcc tttaccttta actcaccaaa ctcgtgggca ccagcggcct
    10081 atgacgcgaa gctggatctg gtctatctgc cgatgggcgt gaccacgccg gatatctggg
    10141 gcggtaaccg cacaccggaa caggaacgtt atgccagctc gattctggcg ctgaatgcca
    10201 ctaccgggaa actggcgtgg agctaccaga ccgttcacca cgacctgtgg gacatggatc
    10261 ttccggcaca gccgacgctg gcggacatca ccgttaatgg tcagaaagtg ccgattattt
    10321 acgctccggc gaaaaccggt aacatttttg tgctcgatcg tcgtaatggc gaactggtgg
    10381 ttccggcccc ggaaaaaccg gttccccaag gtgctgccaa aggcgattac gttaccccta
    10441 ctcaaccatt ctcggaactg agcttccgtc cgacgaaaga tttgagcggt gcggatatgt
    10501 ggggagccac catgtttgac caactggtgt gccgcgtgat gttccaccag atgcgctatg
    10561 aaggcatttt cacgccgcca tctgaacagg gtacgctggt cttcccgggt aacctgggga
    10621 tgttcgaatg gggcgggatt tccgttgatc caaatcgtga agtggcaatt gccaacccaa
    10681 tggcactgcc gtttgtttcg aaactgctcc cgcgtggtcc tggcaacccg atggagcagc
    10741 cgaaagatgc caaaggcacg ggtacggaat ccggcattca gccacagtac ggtgtaccgt
    10801 atggtgtcac gctcaacccg ttcctctcac catttggtct gccatgtaaa cagccagcat
    10861 ggggttatat ctcggcgctg gatctgaaaa ctaatgaagt ggtgtggaag aaacgtattg
    10921 gtacgccgca ggacagtatg ccgttcccga tgccggttcc ggtgccgttc aatatgggta
    10981 tgccgatgct gggcgggcca atctccacgg cgggtaacgt gctgtttatc gccgctacgg
    11041 cagataacta cctgcgcgct tacaacatga gcaacggtga aaaactgtgg cagggtcgtt
    11101 taccggcggg tggtcaggca acaccgatga cctatgaagt gaatggcaag cagtacgtgg
    11161 tgatctccgc aggcggtcac ggttcatttg gtacgaagat gggcgactat attgtggctt
    11221 atgccctgcc ggatgatgtg aaataaccat aatgcccgga gaaatccggg catatttccg
    11281 aatacggtct ttttataccg taaaccctaa catcatcccc gtatcttcat gctccagcag
    11341 atggcagtgc gccatataag catgttcttt cggtgcgttg tgattaaact tcaccagcac
    11401 ttcactgaca ttgccttcta ccttaacggt atctttccag cccgcgcgat gagccgctgg
    11461 cggtttgcca ttttctgaca agatacggaa ctgcgtgccg tgaatatgga acggatgcag
    11521 catcatgtcg cccacgccag agataaccca acgttcgtac tgccctttcg ccgccgcaaa
    11581 catcggctta ttcatatcaa acgcctgacc gttgatttta ttggcatggt ggaaatcgaa
    11641 cttcccgccg tggttcatat gattcatatt gccgtgcccc atatggccca tcatctggct
    11701 gtgatccatt ccggccatcg cctgatcgcc atatttctcc attagcatct gcatccccat
    11761 catatcgagc atcgggtcca tagaaagttg cagcttgcgt accgtcagcc cttccagcga
    11821 aggtaacgca ggcaggctac ttaatgtgtc aggcaaagca ccggaggcac taatagcaat
    11881 cggctgaatc cgcattaccg gatgcggctt gtcaaacggt gcaatcgcca tccccatctg
    11941 gctgaccggc agcgtcacca ggtcaaaggg tttgttgtcg ttaacctcca ccagcacttc
    12001 aaaacgctcg cccatcagca ccggcagctc gctcaccttc accggttcag gtagcagacc
    12061 accgtcgctg gcaatcacat acagcgggcg attgtcgctg gtggcgaaat tgagcgagcg
    12121 ggcattacag ccattgagca aacgcaggcg cagccaacca cgcggggcag cgtgttgtgg
    12181 gtagattgca ccgttggtca gcagcgtatc gccaaaccag cccacggcgg cggtcatcac
    12241 atccagttga taatcaatct gcccgtcggc gctaaatttc ttatcctgaa cgatcaccgg
    12301 aacatcatcg ataccccact gttttggcag cattaatttc aggatctcgt catcttcaat
    12361 caccaccaga ccagccagcc ccatcgccac ctgtcgccgg tcttgccatg ttgatgtgga
    12421 tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg cttgccacct
    12481 ggcggaataa ttccctgtgg gccgccgtcg acgtcacccg gtacttccag cccgtgccag
    12541 tgcaacgtcg tctcttccgc cagttggttg tagatatcaa ccgttaccgc tttgccgcgc
    12601 tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt tgcagttttc
    12661 tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg ggcatcggtc
    12721 gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc acggctccac
    12781 agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc acgacgttgc
    12841 atagttattt ccttattctt aagcaggcaa aacaaaatca aacgttgagc atagtccttc
    12901 cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt cgtcgcctcg
    12961 ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg aagacgtttt
    13021 tcagaacagt gttattcggc agcctgatcg ccgtttgcgc aaacagttac gcgctcagcg
    13081 agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg aagaacgatt
    13141 gtggttacca gaacttacct aacgggcaaa ttcgtcgcgc actggtcttt ttcgcccagc
    13201 aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc ggtgaagaca
    13261 gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa gccctggccc
    13321 gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt tgtagaaata
    13381 ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt tacgggtgtt
    13441 aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg ctacacgacc
    13501 agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc gatcaccagg
    13561 atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat ggcgtagtac
    13621 aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt ccgctactgg
    13681 cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc atgctgcgtg
    13741 aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat gcgggtgtcg
    13801 tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac gatccgcgct
    13861 ttcaactagt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag acctttgatg
    13921 tcattatctc cgactgcacc gatcctatcg gtcccggcga aagcctgttc acctcagcat
    13981 tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca caaaacggcg
    14041 tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc cattacttca
    14101 gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc atgacttttg
    14161 catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt caggcgcgtt
    14221 ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg gcagcttttg
    14281 ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga gaagataaga
    14341 aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg agtttttgta
    14401 tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt gcttatatcg
    14461 atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt tccattatcg
    14521 gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc agcgtcacta
    14581 ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa caccccggcc
    14641 cactgccaga aacggtggtt gctcatctcg ataaaagtca tatttgcgta catacctacc
    14701 cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa gtctctacct
    14761 gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt gagtccgata
    14821 tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga cattaacggt atgaagcact
    14881 ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg aaggcgctgt
    14941 atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag atgttgctta
    15001 aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta accgacagcg
    15061 agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat tacgggcgca
    15121 atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat ttatttactt
    15181 ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa cgccgcatag
    15241 cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc cagcgaattc
    15301 cagttgattg gcgcgaacca tcacctcttc agcgtccatc cacagggtgt attcatgccc
    15361 tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt ccacttcatc
    15421 aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca cttcatgccc
    15481 catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt aatccatagt
    15541 gtgttcctct gctcccggca atgcgccgaa atcgcatcgc gccattgatg taattatcgc
    15601 agccttaccg gagaaaaaca gcggaacggc gatggtttag aaataaaaaa tgcccgggaa
    15661 tctcattcac cgggcattgg tgtcgtttat gcgcagcgcg tgcgcttact tattacaccg
    15721 cagtctggaa aatcacccca tcggcttttt cggtgtactg agaaagctgg ttgaagttca
    15781 gataacgata agtatcaacg gcagttttat ctacttgcgc aacgtaggtc tggtactctt
    15841 ccggcgtcgg cagtttgcca atcagcgccg caacagccgc cagttctgca gaagccagga
    15901 agacattcgc gccagtaccc aaacggttcg ggaagttacg ggtagaggtg gaaaccaccg
    15961 tcgcaccgtc tgctacacgc gcctggttac ccatacacag ggaacagccc gggatctcga
    16021 tacgagcccc gctcttaccg aagacgctgt agtagccttc ttccgtcagt tgcgcggcgt
    16081 ccatacgggt tggcggtgcc acccacaggc gggtcggcaa ctgacctttg tgcgcatcca
    16141 gcagtttacc agcggcacgg aagtgaccga tgttagtcat gcaagaaccg ataaatactt
    16201 cgtcgatctt ctcgccctgt accgcagaca gcggacgtgc gtcatccgga tcgttcggag
    16261 cacacaggat tggctcttta atctccgcca gatcgatgtc gatcactgcc gcgtattccg
    16321 catctgcatc ggcttccagc agctcaggat tcgccagcca tttttccatg ccctgaatac
    16381 ggcgttccaa ggtacgacga tcgccgtaac cttccgcgat catccacttc agcaggacga
    16441 tgttggagct caggtattcg atgatcggtt ctttgttcag cttgatagta caaccagcgg
    16501 cagaacgctc ggcggacgca tcggttagct caaaggcctg ctcaactttc agatccggca
    16561 gaccttcaat ttccagaatg cgaccagaga agatgttttt cttgcctttc ttctcaacgg
    16621 tcagcagacc ttgtttgatc gcatacagcg gaatggcgtg taccagatcg cgcagggtga
    16681 tgcccggctg cattttgcct ttgaagcgca ccagaacgga ttccggcata tccagcggca
    16741 ttacgccagt tgcggcagca aacgccacca gaccagaacc cgccgggaaa gagataccga
    16801 tcgggaaacg ggtatgggag tcaccaccgg taccgacggt atccggcagc agcatacggt
    16861 tcagccagga gtgaatgacg ccgtcacccg gacgcagcga cacaccgcca cggttcataa
    16921 tgaagtccgg cagcgtgtgg tgcgtgttca cgtcaactgg cttcggatac gccgcggtgt
    16981 ggcagaaaga ctgcatcacc aggtcagccg agaagcccag gcacgccagg tctttcagtt
    17041 catcacgggt catcgggccg gtggtgtcct gagagcctac agaagtcatt ttcggttcgc
    17101 agtacgcgcc cggacgaatg cctttcacgc cacaggcacg gcctaccatt ttctgcgcca
    17161 gcgagaagcc gcgatcgctc tcagcgacat ctttcgcctg acggaacaca tcactgtgcg
    17221 gcagaccaag tgcttcacga gctttggtgg tcaggccacg cccgataatc agcggaatac
    17281 ggccgccagc acgcacttca tcaatcagca cgtcggtttt cagttcgaag gtcgccagca
    17341 gttcgcctgt ttcgtggtta cgcacttcac ctttgtacgg gtaaacgtca atcacgtcgc
    17401 ccatgttcag gttagagacg ttgacttcga ttggcagtgc acccgcgtct tccatcgtgt
    17461 taaagaagat gggtgcaatt ttaccgccga ggcacaaacc accgccgcgt ttgttcggca
    17521 catgtggaat atcatcgccc ataaaccaca gaacggagtt cgtggcggat ttacgcgatg
    17581 aacccgtacc cacaacgtca ccgacgtacg ccagcgggaa acctttctgt tgcagagctt
    17641 caatctgttt gatcgggcca acaacacctg gctggtctgg ctcaatacct tcacgggcgt
    17701 ttttcagcat cgccagcgcg tgcagtggga tatccgggcg tgaccacgca tccggtgccg
    17761 gagaaaggtc atcggtgttg gtttcgccag tgactttaaa gacggtaacg gtcagttttt
    17821 cagccagcgc cgggcgattc aggaaccatt cggcatccgc ccaggactgc attacctgct
    17881 tcgcatattc gttgcctgct ttcgctttct cttctacgtc atagaagtta tcgaacatca
    17941 gcagcgtgtg agaaagtgct ttggcagcaa taggtgccag tttggcatca tccagcgcgt
    18001 cgatcagcgg atgaatgttg taaccaccct gcatggtgcc cagcagttcg atagcttttt
    18061 ccggagtcag cagaggggat ttagcttcgc ctttagcgat agcagccagg aagcctgctt
    18121 ttacataggc ggcttcatcg acgcctgggg gaacacggtt ggttaacaga tctaacagga
    18181 attcttcttc gcccgcgggc gggtttttca gcagctctac aagcgcggcc atttggtttg
    18241 catccagggg tttgggcgca atcccctcag cggcacgctc agctacgtgc ttacggtatt
    18301 cttctagcac gacggttctc ctcgctctca ttgtcatagt gcggcaggcg attctcttca
    18361 cgctcctgtg agacagcagt ttgtagggta aatgcccagt accgcaacgg gcagaatagc
    18421 aggattttga cgacgtgtta atctgtttac aaaaaagcaa cataaaaaag tggctgaatc
    18481 gttaaggatg gtctagagat tgtttccctc cgtaaaaact tcgttaattc gcatggtgat
    18541 aatcaccttt ctcaacgaat cccaagatta gtcaaaattt aaactaccgc ctctttatac
    18601 tcggattcac agcacctgcg ggtggcagtt cgccgaccat tgcgatttcc ttgagatccg
    18661 aattatgaaa atgactttgc cgtttaaacc ccatgtgctg gcgctaattt gcagtgccgg
    18721 gctttgtgct gcctctgccg ggctatatat aaaaagccgc acagtggaag cgcctgtaga
    18781 accgcaatcg acacaactgg ctgtgtctga cgctgccgct gttacgcttc ctgcaacggt
    18841 ttccgcacct cccgtaatac ccgcagtcgt caaatccgca ttcagcactg cacaaataga
    18901 tcaatgggtc gcacccgtcg cgctgtatcc cgacgcccta ctttcgcagg tgctgatggc
    18961 atcaacctat ccgacaaacg ttgctcaagc agtgcaatgg tcgcacgata atccacttaa
    19021 acaaggcgat gctgctattc aggcggtatc tgaccagccg tgggacgcca gcgttaaatc
    19081 actggtggcc tttccacaat tgatggcatt gatgggcgaa aacccgcaat gggtgcaaaa
    19141 cctgggcgat gcttttctgg cgcagccgca ggacgtgatg gactcggtac agcgattgcg
    19201 gcaactggcg caacaaaccg gctcgctgaa gtcatcaacc gaacagaaag ttattaccac
    19261 aacgaagaaa gctgtaccgg taaaacagac agtcacggca cccgtcatac catccaatac
    19321 cgttttaact gccaaccccg tcattacaga gccggcaaca accgtcattt ccattgagcc
    19381 cgccaatcct gatgtggtct atattcccaa ctacaaccca accgtggttt acgggaactg
    19441 ggccaatact gcgtatccgc cggtttatct gccaccacca gccggagaac cgtttgttga
    19501 cagctttgtg cgcggattcg gctatagcat gggcgttgct accacgtacg cactattcag
    19561 cagcatcgac tgggatgacg acgatcatga ccatcatcat catgacgatg atgattatca
    19621 tcaccacgat ggcggtcatc gtgacagtaa tggctggcaa cacaacggcg acaacatcaa
    19681 tatcgacgtc aacaatttca acagtatcac cggtgagcat cttactgata agaatatggc
    19741 atggcggcac aatccaaact accgtaatgg tgtgccctat catgatcagg atatggcaaa
    19801 gcggtttcat caaaccgatg tcaacggcgg aatgagtgcc acgcagctac ctgctccaac
    19861 acgcgacagc cagcgtcagg cgacagcaag tcagtttcag caacgaacac acgccgcccc
    19921 cgtcattaca cgagataccc aacgtcaggc agcggcacag cggtttaatg aagctgaaca
    19981 ctatgggagc tatgacgact tccgcgactt cagccgtcgc caaccactga cccagcagca
    20041 aaaggacgcc gctcgtcagc gttatcagtc ggcctctcct gagcagcgcc aggcagtccg
    20101 cgagaaaatg cagactaacc cgcagatcca gcagcgaagg gaggcagcgc gccagcgtat
    20161 tcagtccgcc tcgcctgagc agcgccaggc ggttcgcgag aaaatgcaga ctaacccgca
    20221 gaaccagcag cgaagagagg cagcgcgtga gcgtattcag tctgcaactc ccgaacagcg
    20281 ccaggtgttt aaggaaaaag tacagcagcg cccactgaac caacagcaac gtgataacgc
    20341 ccgccagcgt atacaatcgg catcacctga acaacgtcag gtttttcggg agaaagttca
    20401 ggagagccgc ccacaacgtc taaacgacag taaccatact gtcaggctga ataacgagca
    20461 acggtcaaca gtacgcgaac gtctctctga acgtggagca agaagactgg aaaggtaaat
    20521 tgcagacgta aaaaaagcgg cgtggttagc cgctttttta attgccggat gttccggcaa
    20581 acgaaaaatt acttcttctt cgctttcggg ttcgggttcg gcaggtcggt aatgctacct
    20641 tcgaacactt ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc gtggatggtc
    20701 agtgcgatgt cttcagcatc acaacccatt tcgattgcca gcccgatttc acccagcagc
    20761 tcgccgccgt tggtaccaac aatcgcacca ccgatcacgc ggtgagattc tttgtcgaaa
    20821 atcagcttgg tcataccgtc tgcgcagtca gaagcgatag cacgaccaga agcagcccac
    20881 gggaaggtgg cggtttcata gctgatgcct ttctctttcg cttctttctc agtcagacct
    20941 acccatgcaa cttctggttc ggtataggcg atggacggga taactttcgg atcgaagtag
    21001 tgtttcttac cggcgataac ttcagcggca acgtgacctt cgtgaacacc tttgtgtgcc
    21061 agcatcggct gaccgacgat atcgccgata gcaaagatgt gcggtacgtt ggtacgcagc
    21121 tgtttgtcaa cgcggatgaa accacggtcg tcaacttcca cgccagcttt gcctgcgtcg
    21181 aggtttttac cgttcggcac acgaccaatc gctaccagca cggcgtcgta acgctgcggt
    21241 tcagctggtg cttttttgcc ttccatcgtc acataaatac cgtcttcttt cgcttcaacg
    21301 gcggtaactt tggtttccag catcaggttg aatttcttgc tgatacgctt ggtgaagact
    21361 ttaacgatgt ctttgtcagc tgccgggata acctggtcga acatttcaac cacgtcaatc
    21421 tgtgaaccca gcgcgtggta aacggtgccc atttccagac cgatgatacc gccacccatt
    21481 accagcaggc gttctggcac ttctttcagt tccagcgcgt cagtggagtc ccagatacgc
    21541 ggatcttcat gcggaataaa cggcagttgg atcgggcgag aacccgctgc aatgatcgca
    21601 ttgtcgaagt tgatcacggt tttgccgttc tcaccttcaa cttccagggt gttagccccg
    21661 gtgaatttac ccagaccgtt gaccactttg actttgcggc ctttcgccat accagccaga
    21721 ccaccggtca gctgattgat cactttctct ttccaggtac gaatcttgtc aatatcggtt
    21781 ttcggttcgc cgaagacgat accgtgttca gccagcgctt tggcttcttc gataactttt
    21841 gctacgtgca gcagtgcttt agaagggata cagccgacgt tcaggcaaac accgccaagg
    21901 gtgttgtaac gttctacgat tacggtttcc agacctaaat cagcgcaacg gaaggcagcg
    21961 gagtaacctg cggggcctgc cccaagtacc acgacctgag ttttgatttc agtactcatc
    22021 atgacctcta tatatttatc tccggcggtc atacccgtcg tctttcaggc cgcagcggcg
    22081 ttagcttcac gccctcaccc cagtcactta cttatgtaag ctcctgggga ttctggcgct
    22141 tgctaccttg ctgcacccag aaatccatag ggtatgtgaa gtaataccct aaccaccacc
    22201 gggtcgttct atccgtcggt attttacaaa attgttaaca atttttaaac aacaaacggc
    22261 aaccgatttg tctattcgct aataacctca atacattcat gagattacca gaaaaaagcc
    22321 ggccgttggg ccggctcttt tacttacatc accagacggc gaatgtcaga cagcgtgttg
    22381 ttaatgatgg taatgaaacg ggcaccatca gcaccgtcga tcacgcggtg gtcgaaggag
    22441 agagaaatcg gcagcatcag acgcggcacg aactctttac cattccacac cggctccatc
    22501 gcggacttgg aaacgccgag gatagccact tccggcgcgt tcacaatcgg cgcgaagtgg
    22561 gtagtaccca ggccgccgat gctggagatg gtgaagcaac cgccctgcat ttcgcccgca
    22621 gtcagcttac cgtcacgcgc tttcttagaa atagtcatca gctcgcgaga cagctcgatg
    22681 atgcctttct tgttgacgtc tttgaatacc ggaacaacca gaccgttcgg ggtatccacc
    22741 gccacaccga tgttgatgta tttcttcagg gtcagacgct gaccgtcttc cgacagcgaa
    22801 ctattgaagc gaggcatctg ctcaagagct gcagcaacgg ctttcatgat gaagacaacc
    22861 ggggtgatct tcacatccag cttacgtttc gccgcttctt cgttctgctg tttacggaac
    22921 gcttccagct cggtgatatc ggttttgtcg aagtgggtaa catgcgggat catcacccag
    22981 ttacggctca ggttagcacc agaaattttc tggatacggc ccagttccac ttcttcgatt
    23041 tcgccaaact tgctgaagtc caccttcggc cacggcagca tgccagggat accaccgcca
    23101 gtcgccgccg gagctgcttc tgcacgtttg atagcttctt tcacgtaagc ctgaacgtct
    23161 tcgcgcagga tacgaccttt acggccagtg cccttcactt tcgccaggtt aacgccaaac
    23221 tcgcgtgcca gacggcggat cagcggggtc gcgtgaacgt aagcgtcgtt ttcagcaaat
    23281 tcagatttgc cttccgcttt cgcagccgga gctgctgccg gagcttcagc ttttgctgcc
    23341 ggagccggcg ctgccgcttc ctgtttcgca ggagctgccg caggtgctgc gccttcaact
    23401 tcgaagatca taatcagcga accagttttc actttatcgc caacgttgac tttcagttcc
    23461 ttcacgacgc ctgcaaacgg agccggaact tccatagaag ctttatcgcc ttctacggta
    23521 atcagtgact gttcagcggc aactttgtcg cccactttca ccatcacttc ggtcacttca
    23581 acttcgtcac cgccgatatc cggaacgtta acttctttca cgccagctgc tggtgcgggg
    23641 gtcgctgccg gagtcgcttc ctgtttagcc gccggagctg ccgcgcctgc ttcacccgcg
    23701 acttcgaaga ccataatcag cgagccggta gacactttgt cacccacgtt cactttgatc
    23761 tctttcacgg tacctgcaaa cggagccgga acttccatag aagccttgtc gccttctacg
    23821 gtgatcagcg actgttcagc ttcaacttta tcgccaactt tcaccaggat ttcggtcact
    23881 tcaacttcgt cgctgccgat atccggaacg ttaacgtctt ttgccgccgc agccgcgggt
    23941 gctgctaccg gagctgcttc tttcttctct tctgcctgag caggtgcagc gtctgctgca
    24001 ccgtcggcgg aatcgaaaat cataatcagt gcgccggtct gggttttatc gccaacagag
    24061 actttgatct ctttaacgat acccgcctgc ggagatggaa cttccataga ggctttgtcg
    24121 ccttctacgg tgatcagcga ctgttcggct tcaactttgt cgcccacttt gaccaggatc
    24181 tcggtgattt caacttcatc agccccgatg tccggtactt tgatttcgat agccattatt
    24241 cttttacctc ttacgccaga cgcgggttaa ctttatctgc atcgatgttg aatttggcga
    24301 ttgcgtcagc aaccactttc ttatcgattt cgccacgttt agccagttcg cccagcgccg
    24361 caaccacgac ataagaagca tcaacttcga agtggtgacg caggttctca cggctgtcgg
    24421 aacgaccgaa gccatcagta cccagtacgc gatagtcgtc agccggtacg taagtacgga
    24481 cctgctcagc gaacagtttc atatagtcgg tagatgccac tgccggcgcg tcgttcatca
    24541 cctgagcgat atacggaacg cgcggagttt ccagcggatg cagcatgttc cagcgttcac
    24601 aatcctgacc atcacgcgcc agctcggtga aggaggtcac gctataaacg tcagaaccta
    24661 cgccgtaatc tttcgccagg atctctgccg cttcacggac gtgacgcagg atggaaccgg
    24721 agcccagcag ctgaacttta cctttgctac cttcaatggt ttcgagtttg tagatacctt
    24781 tacggatacc ttcctcagca ccttccggca ttgccggcat gtggtagttt tcgttcagcg
    24841 tagtgatgta gtagtaaacg ttctcttgtt tttcaccgta catacgctcc agaccgtcat
    24901 gcataatgac agcaacttcg taagcgtaag ccgggtcgta agagatacag ttcgggatag
    24961 tcagcgactg aatgtggctg tgaccatctt cgtgctgcag accttcaccg ttcagggtgg
    25021 tacgaccgga agtaccgccg atcaggaagc cacgcgcttg ctggtctcca gccgcccagc
    25081 acagatcgcc gatacgctgg aagccgaaca tcgagtaata gatgtagaat gggatcatcg
    25141 gcagattgtt cgtgctgtaa gaggtcgccg ctgccagcca ggaacaccct gcgcccagct
    25201 cgttgatccc ttcctgcaga atctgacctt tctcgtcttc tttatagtaa gcaacctgct
    25261 cgcggtcctg cggggtgtac tgctgaccgt tcgggctgta aataccaatc tgacggaaca
    25321 gaccttccat accgaaagta cgcgcttcgt cggcgatgat cggtaccaga cgatctttga
    25381 tcgacttgtt cttcagcatc acgttcagag cacgaacgaa agcgatagtg gtagagagct
    25441 ctttgctctg ctcttccagc agcgcgccga agtcttgcag gctcggcagc tcaagcttct
    25501 cggtgaagtt cggctgacgg cttggcagat aaccgtgcag tttctgacgc tgagcgtgca
    25561 gataggtatg ctcttcagaa ccttccggga aggtgatgta cggcagtttt tcgatatctg
    25621 catcagacac cggcacattg aaacggtcgc ggatgtgacg cacgccgtcc atgttcattt
    25681 tcttaacctg gtgcgcgatg tttttacctt cagccgcgtc gcccatgccg taacctttaa
    25741 tggtatgagc aaggattact gtcgctttgc ctttggtttc ctgcgctttc ttgaatgcag
    25801 cgtagatttt cttcggatcg tgaccaccac ggttcagtgc ccagatctgc tcgtcagtcc
    25861 agtctgcaac cagtgctgcg gtttcaggat atttaccgaa gaagtgttca cgaacgtacg
    25921 caccatcttt cgatttgaag gtctggtagt cgccgtcaac ggtttcgttc atcagctgga
    25981 tcagtttacc gctggtatct ttacgcagca gttcatccca acggctaccc cacatcactt
    26041 tgatcacgtt ccagccagca ccttcgaaga tgccttccag ttcgttgatg atcttgccgt
    26101 taccggtgac cgggccgtca agacgctgca ggttacagtt gataacgaaa accaggttat
    26161 ccagtttttc acgggtagcg atggtgatcg cacctttgga ttccggttcg tccatttcac
    26221 cgtcgccgag gaacgcgtaa acggtctgtt tagaggtatc tttcaggcca cggtgttcca
    26281 gatatttcag gaatttagcc tggtaaatag caccaatcgg acccagaccc atagatacgg
    26341 tcgggaactg ccagaattcc ggcatcagtt tcgggtgcgg ataggaagag aggccattgc
    26401 cgtgaacttc ctgacggaag ttatccagct gctcctgagt cagacgacct tccaggaaag
    26461 cacgggcgta aacgcccggg gagatgtggc cctggaagta aaccaggtcg ccgccatcct
    26521 gctcgttgcg tgcacggaag aagtggttaa agcacacatc ataaatggtt gcggaagact
    26581 ggaaggacgc catgtggccg cccagttcga ggtctttttt cgacgcacgc agcaccgtca
    26641 tgatggcgtt ccagcggata gctgaacgaa tacggcgttc cagttccaga ttacccggat
    26701 actccggttg ttcttcaacg gggatggtgt tgatgtagtt gctgatacct gtgcctgcgg
    26761 ctacgtttac accgcctttg cgggcttcag caagcagttg gtcgatcaga tactgagcac
    26821 gctcaacacc ttcttcacgg atgaccgatt cgatcgcctg gagccagtcg cgagtttcga
    26881 tcggatccac gtcatttggg aaacgttctg acatgggtta ttccttatct atctaataac
    26941 gttgagtttt ctggaacctg tcccattgaa ctctcgccag aaagctcaat aagacaggtt
    27001 ctacgtttag ttaccgcgct tttatatgcg cttgatttac aacatcttct ggataatttt
    27061 taccagaaaa atcactaatt ctttcgttgc tccagacgac gcagagaacg ctcacggcgg
    27121 ctctcttcac ggcttctgtc gagcaaaatt tcttcgataa aggccagatg gcgatgcgat
    27181 gcttcgcgcg cttcttccgg cttaccggcc ataatcgctt caaatatgcg ggtgcggtga
    27241 ctactcacca gcggcagcat ctcgcgacgc gaatagagca attcgaagtt ctggcggaca
    27301 ttctgggcca acatcggctc catacacctt agcagatgaa gcagaaccac attgtgggcc
    27361 gcttcggtga cggcaatctg atactggagt acggcgtttg attccgcgtc cagatcgcca
    27421 gactgctgcg ccagctctat ggcgtggtgg agttcacgga tgcgttcctt gtcttcatcg
    27481 gtactacgca gcgcggcgta ataagcggcg ataccttcca gggcgtgtcg tgtttcgagc
    27541 aagtcatact gtgattcagg atggtcggag agcagctcca ccagcggatc gctgaagctt
    27601 tgccataggc tgctctggac aaaagtgccg ccaccctggc gacgaagcaa caagcccttc
    27661 gcttcgagac gttgaatcgc ctcacgcaag gagggacggg agacgtcgaa ctgttttgcc
    27721 agttcgcgtt ccggtgggag tttttcgccc gggcggagag tgccttcgag gatcaaaaac
    27781 tccagttgct gctcaatcac atcggagagt tttggttggc ggattttgct gtaggccatg
    27841 agttcctgtc ttaagccact tgccgaagtc aattggtctt accaatttca tgtctgtgac
    27901 gctaaagtaa caaagtattc accttatgtc catacaggtt ttgattgaaa tcatgaaact
    27961 gtgcacattt taacaacttg acatatataa cgtttcaaag ttgtaactat gcacaaatgt
    28021 agactttacg taggaaaggg attttttaac gattattaaa ctataaaaat ccgaattgaa
    28081 ccgattcact taccaatttt ttgattttta agtcaataaa aacgaattta aattcactct
    28141 gcatattgag aggggttgag gctgagcttt acaaacggtt tctttttaag caactcatct
    28201 tcaaccatgc ataaagcggg tgcattcgct gccgcatacc attattcttg atctgacgga
    28261 agtctttttg taacaattca aacttccttg atgtaaataa atcaatacaa caaacggaat
    28321 tgcaaactta cacacgcatc actgcgtaga tcaaaaaaac aatcaccgca cgaggtttca
    28381 tgatggaagg tcaacagcac ggcgagcagc taaagcgcgg ccttaaaaac cgccatattc
    28441 agcttatcgc gctgggtggc gcgataggga cagggctatt cctgggtagc gcctccgtaa
    28501 tacagtccgc agggccaggg attatcctgg gttacgccat tgctggtttt atcgcctttc
    28561 tgatcatgcg tcagctgggt gaaatggtgg tcgaagaacc tgtcgcaggc tcctttagcc
    28621 actttgctta taaatactgg ggcagttttg ccggtttcgc ctctggctgg aactactggg
    28681 tactgtacgt tttagttgcc atggcagagc tgactgccgt gggtaaatac attcagttct
    28741 ggtatccgga aatcccaacc tgggtttctg ccgccgtatt ctttgtggtg attaacgcca
    28801 tcaacctgac caacgttaaa gtgtttggcg agatggagtt ctggtttgcc attatcaaag
    28861 ttattgcggt agtagcgatg atcatcttcg gcggctggct gctgttcagt ggcaacggcg
    28921 gcccgcaggc gagcgttagc aacctgtggg atcaaggtgg tttcctgccg cacggcttca
    28981 ccggactggt gatgatgatg gcgattatca tgttctcgtt cggtggtctg gaactggtgg
    29041 ggatcaccgc agcagaagct gataacccgg agcaaagtat cccgaaagca actaaccagg
    29101 ttatctaccg catcctgatt ttctatattg gttcgttagc cgttctgctc tcactgatgc
    29161 cgtggacccg cgttaccgcc gataccagtc cgtttgtgct gatcttccac gagttaggcg
    29221 atacctttgt ggcgaatgcg ctgaacatcg tggtactgac tgcggcgctc tccgtgtaca
    29281 acagctgcgt atattgcaac agccgtatgc tgtttggtct ggcacaacag gggaacgcgc
    29341 caaaagcgct ggcgtctgtc gataaacgcg gcgtaccggt taacaccatt ctggtgtctg
    29401 cgctggttac agcattgtgc gtattgatta actatcttgc tccggaatcc gcatttggcc
    29461 tgttaatggc actggtggta tccgcactgg tgatcaactg ggcgatgatc agtctggcgc
    29521 atatgaagtt ccgtcgcgcc aagcaggaac aaggcgtggt aactcgcttc cctgctctgc
    29581 tttatccgct gggtaactgg atctgcctgc tgtttatggc ggcggtactg gtgattatgc
    29641 tgatgacccc aggaatggcg atttcggtat acctgatccc ggtatggctg atcgtgttag
    29701 gtatcggcta tctgtttaaa gagaaaaccg ccaaagccgt aaaagcgcat taatctctct
    29761 acgccctcac ccgtacaggg tgagggcaat aatctctaca ccaacgcccc gtaaatcgtc
    29821 agtagcgcaa tcaccaccac gaccacgaac gaggttttct tcgccattga aaccgctgcc
    29881 ttcggcgtct ccaccttatc gacatgcggt tcacgcgcca gagagaactg cgccagacgc
    29941 gttaatacct gatattgcga agtatggaaa tcacccagcg aagcaaacca ggccggtaac
    30001 gctttctcac catgaccgat caaggcatat accacacccg caagacgaac cggcacccaa
    30061 tccagtacat gaagcactgc atcaatgcca gactgtaaac gatgatgcgg cgtctggtat
    30121 cgcgccagcc agtattgcca tgcacgcaag aacgcatacc ccatcagcgt aacgggtccc
    30181 caggttcccc ccacaatcag ccagaacagc ggtgcaagat aaaaacgaaa gttaatccac
    30241 agcaatgcat tttgcagctc acgcaaatac tcacgttcgt cgcagcctgc cgggacgccg
    30301 tgaatcatgg tgagttcgcc agccatcgtg gcacgggcat ggctatcatt acgtgaagca
    30361 gctgtcagat aagcatgata atgaagacga actttacctg cgccaataca cagcaaacca
    30421 atcagcagcc acaccagcag cgtgggaacg ttgaacaata ctccctgcaa tgcgcgtaac
    30481 agtaaaaaag tcacacccat cgcaataatg gtcatgccta acgtgcgccc gagagaaaaa
    30541 tgtttcaccc gccgaaagaa cgcttcaaga cgatgatcaa gctgccagtg ctcgcccaac
    30601 ttaaacaggc gctcgaaaat taacaccagt aaggttgtaa atagcgtcat gttgtctcct
    30661 tgctgaccag cgcacgaaac cgtgcccaat caaatgcagg accgggatcg gttttccgat
    30721 ccggcgcaat atcacaatgg cccgtcatgt tattagcgat atcgggatag cgatctatca
    30781 gtgcccgcgt aaccgccgca agctgttgat actgcgcatc ggtatacgcc agagtatcgg
    30841 tgccttcaag ctcaatccca atagaaaaat cattgcagcg ttcacgcccc tgatactgag
    30901 agactcccgc atgccaggca cgtttatcga aaggaacata ctggactatt tcaccatcac
    30961 ggcgaatcaa acagtgagcg gagacgcgca aatgggcgat ctcagcaaag aaaggatgtg
    31021 cctgcggatc aatagttcca gtgaataatg cgtcgatcca cggaccgcca aactcgcctg
    31081 gcggcaggct aatattgtgc accaccagca gggtgggtgt ttcgtcatcc aggcggcaat
    31141 cgtaatgtgg tgagggaacg cggcgcgcgc caaccagcca cccctgttct aacaacatgc
    31201 aggatctcct tatatgtggt gctaataccc ggttcagagt agcatgtttc taccttatga
    31261 ttcgttagct atctggagtt ttaacatgcc gcctcgccgc tacaaccctg acacccgacg
    31321 tgacgagctg ctggaacgca ttaatctcga tatccccggc gcggtggccc aggcgctgcg
    31381 ggaagattta ggcggaacag tcgatgccaa caatgacatt acggcaaaac ttttaccgga
    31441 aaattctcgc tctcatgcca cggtgatcac ccgcgagaat ggcgtatttt gtggcaaacg
    31501 ctgggttgaa gaggtgttta ttcaactggc aggcgacgat gtcaccataa tctggcatgt
    31561 ggatgacggc gatgtcatca acgccaatca acccttgttc gaacttgaag gcccatcccg
    31621 cgtgctgtta acgggcgaac gcactgcgct taattttgtg caaacccttt caggagttgc
    31681 cagtaaggta cgccactatg tcgaattact ggaaggcacc aacacgcagt tgttggatac
    31741 gcgcaaaacc ttacctggcc tgcgttcagc tctgaaatac gcggtacttt gcggcggcgg
    31801 agcgaatcac cgtctggggc tttctgatgc cttcctgatc aaagaaaacc atattattgc
    31861 ctccggctca gtgcgccagg cggtcgaaaa agcgtcctgg ctgcatccgg atgcgccagt
    31921 agaagtcgaa gtagagaatc tggaagaact tgatgaggcc ctgaaagccg gagccgatat
    31981 catcatgctg gataacttcg aaacagaaca gatgcgcgaa gccgtcaaac gcaccaacga
    32041 caaggcgcta ctggaagtgt ctggcaacgt cactgacaaa acactgcgcg aatttgccga
    32101 aacgggcgtg gactttatct ccgtcggtgc gctaactaaa cacgtacaag cactcgacct
    32161 ttcaatgcgt tttcgctaat tgctgacaga tttatccgct ccatcgcggg cggataatct
    32221 ccccttcccc aactttcttc gtaacgcctc gcaaatttct tgtctttcgc tgcaacgaaa
    32281 taaaaaacct cggaatctgc tttcccgttt cgttttttgc ccctcgcctg tgctgatggc
    32341 gtttgccaaa gtagcaccaa ccaaatcaag gagcgaaaca gatggacaag caacgcggtt
    32401 ttacacttat cgaactgatg gtggttattg gcatcattgc cattttaagc gccattggta
    32461 ttcccgctta tcaaaactac ctgcgcaaag ccgcactcac cgacatgcta caaacctttg
    32521 tgccttaccg taccgccgta gagttgtgcg cgctggaaca tggtggatta gatacctgcg
    32581 acggtggcag caatggcatt ccctcgccta ccaccacccg ctatgtttca gccatgagtg
    32641 tggcaaaggg cgtggtgtcg ctgaccgggc aagaaagtct caatgggcta agcgtcgtca
    32701 tgacgccagg ttgggataac gcaaacggcg tcaccggctg ggcgcgcaac tgcaatattc
    32761 aaagtgacag cgcattgcag caagcctgcg aagatgtctt ccgctttgat gacgccaact
    32821 aaggagcggc aatgaatatt ccacagctca cggccctgtg cctgcgttat cagggagtct
    32881 tgctggatgc cagcgaagaa gtggttcatg ttgcggtggt cgatgccccc tcacatgagt
    32941 tgctggacgc attgcatttc gctaccacca aacgtattga gatcacctgc tggacgcgcc
    33001 aacaaatgga aggtcacgcc agtcgcacac aacagacatt gcccgtagct gttcaggaga
    33061 agcatcagcc caaagcagag ttgctgactc gaacgttaca atctgcgctg gaacaacgcg
    33121 cgtctgatat tcatatcgaa ccagcggaca atgcctaccg catccgcttg cgtatcgacg
    33181 gcgtattgca tcctttaccg gatgtttcac cggatgccgg agtcgcatta acagccagat
    33241 taaaagtgct gggaaacctg gatattgcgg aacatcgcct gccgcaggac gggcaattca
    33301 ctgtcgaact ggcaggaaac accgtctcat ttcgtattgc gaccttacca tgtcggggtg
    33361 gtgaaaaggt ggtattaagg ttgttacagc aggtgagcca ggcactggat gttaacacgc
    33421 tgggaatgca gccgttacaa ctggcggact ttgctcatgc cttgcaacaa ccacagggac
    33481 tggtgctggt aactggccct acaggcagcg gcaaaacggt cacgctttat agtgccctgc
    33541 aaacgctgaa taccgctgac attaatattt gtagcgtcga agatccggtt gagatcccca
    33601 tagccggact aaaccagacg caaatccatc cgcgtgccgg gctcaccttt cagggtgttt
    33661 tgcgtgcgtt attgcgccag gatcctgacg tcatcatgat cggagagatc cgcgatggcg
    33721 aaacggcaga aattgccatt aaagccgtgc aaaccggtca cctggtgttg tccacgctac
    33781 acaccaattc caccactgaa acgctggtac gtttacagca aatgggggtt gcccgctgga
    33841 tgctatcatc ggcgcttacg ctggtaatag cccagcgtct ggtacgtaaa ctttgcccac
    33901 attgtcgccg acagcaaggg gagcccatcc atattccaga cactgtatgg ccgtcgccgc
    33961 tgccccactg gcaggcaccc ggttgtgtac attgctacca cggtttttat ggtcgtaccg
    34021 ccttgtttga agtcctgccc attacaccag ccatacgcca gcttatttcc gctaatactg
    34081 aagttgaatc gctggaaacg cacgcccgac aggcgggtat gcgtacgctt tttgaaaacg
    34141 gctgcctggc cgtggagcaa ggcttaacca cctttgaaga gttaatccgc gtactgggga
    34201 tgccgcatgg caagtaagca actctggcgc tggcatggca taaccggcga cggcaatgcg
    34261 caagatggga tgctatgggc agagagccgt actttactgc ttatggcact acagcaacag
    34321 atggttaccc cactaagcct gaagcgaatc gccatcaatt ctgcgcagtg gcgaggagat
    34381 aaaagcgcgg aagtcattca tcaactggcg acgctactta aagccgggtt aacgctttct
    34441 gaagggctgg ctctgctggc ggaacagcat cccagtaaac aatggcaagc gttgctgcaa
    34501 tcgctggcgc acgatctcga acagggcatt gctttttcca atgccttatt accctggtca
    34561 gaggcatttc cgccgctcta tcaggcgatg atccgcacgg gtgaactgac cggtaagctg
    34621 gatgaatgct gctttgaact ggcgcgtcag caaaaatccc agcgtcagtt gaccgacaaa
    34681 gtgaaatcag cgttgcgtta tcccatcatc attttagcga tggcaatcat ggtggttgtg
    34741 gcaatgctgc attttgttct gccggagttt gccgctatct ataagacctt caacacccca
    34801 ctaccggcac taacgcaggg gatcatgacg ctggcagact ttagtggcga atggggctgg
    34861 ctgctggtgt tgttcggctt tctgctggcg atagccaata agttgctgat gcgccgcccg
    34921 acctggctta tcgcgcggca gaaattgctg ttacgcatcc cgattatggg ttcactgatg
    34981 aggggacaaa aactcacgca gatctttacg attctggcgc tgacacaaag tgcaggcatt
    35041 acttttttac agggcgtaga gagcgtcaga gaaacaatgc gctgcccgta ctgggtgcaa
    35101 cttctgacac aaatccagca cgatatcagt aacggtcatc ccatctggct ggcgctaaaa
    35161 aatgccggtg agtttagccc gctctgtttg caattagtga gaacaggaga ggcatccggc
    35221 tcgctggacc tcatgttaga caacctcgcc catcatcatc gggataacac aatggcgctg
    35281 gcggataacc tcgcagcctt actggaaccg gcgttgctga tcataacggg aggaattatc
    35341 ggtacgctgg tggtggcaat gtatctgcca attttccatt taggcgatgc gatgagtggg
    35401 atgggataac gctggcgtgt tgctccacgc cagcgttggg agattacagg ttgttgaaga
    35461 tgcggttttc ttgttccagc acacgaataa acgtggtgcg cttggtcagc tctttcaggc
    35521 gtgaagcccc aacgtatgta caagctgaac gcaggccgcc caaaatatca cgcgcggtat
    35581 tttcaaccgg gcctcgcagc ggcagcttga cggttttacc ttctgctgcg cggtattccg
    35641 caacgccgcc aacgtgacgt ttcatcgcag actcggagct catgccgtag aacagcataa
    35701 atttctcgcc gttctcctca acgatgcgac cgccgctctc ttcgtggccc gccagcatgc
    35761 cgccaagcat gacgaaatcg gcaccgccgc caaaggcttt cgccacatcg cccggcgtgg
    35821 tacagccacc gtcgctgacg atcattccgc ccagaccgtg cgccgcatcg gcacattcga
    35881 ttaccgcaga aagctgcgga taaccgacgc ctgttttgac gcgagttgta caaacagaac
    35941 ctgggccaat gccaacttta acgatatcag caccggagag gataagctcc tcacacattt
    36001 caccagtcac tacgttgcca gcacaaatgg ttttggtcgg ccacgcttca cgcgctttcg
    36061 caacgaactg cacgaagtgt tcggaataac cattcgccac atcaatacaa acgaagttta
    36121 atgccgggtt caggtcgaga atctgtttag ttttttcgaa atccgcatca gacgtgccgg
    36181 tagaaaccat cacatgtttc agcacatcag cggaagaatt gttgataaac gcttgccact
    36241 cttcgacaga aaagtgttta tgcacagcag tcagaatatc aaaagaagcc agcgcagagg
    36301 ccatagaaaa tgtgcctacg gtgtccatat ttgcggcgat aatcggcacg ccggaccagc
    36361 tctgacctga atgtttgaag gtgaattgac gttccagttc aacatcggaa cggcttttaa
    36421 gagtggagcg tttagggcgg atgagaacgt ctttaaaacc taacttcaga tcttcttcaa
    36481 tacgcatgtg cggattcctg gggttaatgg ctataagggt aagagcgcaa ctccagtgac
    36541 gctatcatac gcactaatca atgcggcgca agactgcgaa attgtctttt tttacgctac
    36601 aatcccataa atttacctgg taaatagtga gcgaacgttt catcaatttc actttcgcgc
    36661 gctcattttt aacgttttga taatcatgat gaattcccgg gaatataaat tatggggtat
    36721 atagttgcct taacgggagg cattggcagt ggtaagagta ccgttgccaa tgcgtttgct
    36781 gatctcggaa ttaacgtcat tgatgccgat attattgcgc gtcaggtggt tgaaccaggt
    36841 gcacctgcgc tacatgccat tgctgatcac tttggcgaaa acatgattgc tgctgatgga
    36901 acattgcagc gccgggcctt gcgcgagcgg atcttcgcca acccggaaga gaaaaactgg
    36961 cttaacgacc tgctgcatcc gctgattcag caagagacgc aacaccagat ccagcaagca
    37021 acttccccct atgtactgtg ggttgtgcca ttgctgatag aaaactcact gtataaaaaa
    37081 gcgaatcgag tgctggtggt ggatgtcagc ccagaaacgc aacttaagcg caccatgcag
    37141 cgcgatgatg taactcgcga acatgtcgaa caaatccttg ctgctcaggc aacgcgcgaa
    37201 gcccgccttg ccgtggcaga tgacgtcatt gataataacg gcgcaccgga tgctatcgca
    37261 tcggatgttg cccgcctgca cgcacactat ttgcagcttg cgtcgcagtt tgtctcacag
    37321 gaaaaaccgt aatgcagacc caggtccttt ttgaacatcc actaaatgaa aaaatgcgta
    37381 catggctgcg cattgagttt ttgattcagc aactcaccgt taatttaccc atcgttgacc
    37441 acgctggcgc gctgcatttc ttccgtaatg tcagtgaatt actggatgtt ttcgagcgcg
    37501 gcgaagtccg cactgagctg ttgaaagaac ttaaccggca gcaacgtaaa ctccagacct
    37561 ggattggcgt gcctggcgtg gaccagagcc gtattgaagc attaattcag cagttaaaag
    37621 cggcggggag cgtattaatt tccgcgccgc gtatcgggca atttctgcgt gaagatcgtt
    37681 tgattgctct ggtgcgtcag cgactgagca tcccaggcgg ctgttgcagc tttgatttac
    37741 ctacattgca catttggctg catctacccc aggcgcagcg cgacagccag gtagaaacct
    37801 ggattgccag cctgaacccg ctcacccagg cacttaccat ggtgctggat ttaattcgcc
    37861 agtcggcccc cttccgtaaa caaaccagcc tgaatggttt ttatcaggat aacggtggcg
    37921 atgccgactt gctgcgcctg aatctgtcgc tcgattcaca gctttatccg caaatttccg
    37981 gacataagag ccgttttgcc attcgtttta tgccgctgga cactgaaaac ggacaggtac
    38041 cggaacgtct ggatttcgaa ctggcctgtt gctaaggagt aaaaatgtca gaaactatta
    38101 cggtgaattg cccaacctgc gggaaaacgg tggtgtgggg tgaaatcagc ccgtttcggc
    38161 cattttgctc caaacgttgt cagctgatcg acctcggaga atgggctgct gaagaaaaac
    38221 gaatccccag cagtggcgat ctttccgaaa gcgatgactg gagcgaagag ccaaagcagt
    38281 gacatttgtc tgatgcctca cgtaggcctg ataagacgcg gacagcgtcg catcaggcat
    38341 cttgtgcaaa tgtcggatgc ggcgtgaacg ccttatccga cctacagacg cttaagcttc
    38401 gcaattaccg gttcattggc tggcggaaaa tcatcggcat taagaccgac cagcgacatc
    38461 cactcaccgg gttgcccttc tttaccccac ggcacccctt cccaactttc gaccagccag
    38521 aaccacagag ttatatgcct gtccgggaat tcatattcca gtttttcaaa tagcgaaaaa
    38581 tgttgtgggg taatcccgac ctcttcctga agttcacgca ccaccgcctg ttccggcgtt
    38641 tcgcccattt caattttacc gccgggaaac tccagtttat tcgccatgtg cgcatctgct
    38701 gcgcgacgtg ttataaagat ttcattgttc tcgttgcgaa taatacctac cgcaatttgc
    38761 agctttttca ttgtcttaaa cctataaaaa aggcgcagaa tcctgcgcct tttacttcaa
    38821 ctgttagctt ttattgcagg cgaccatggc actgcttgta ttttttacca gaaccgcacg
    38881 ggcaaggatc gttacgtcct actttgcgtt caccggtttg cgccgccagt gcagctgcgg
    38941 ctgcagagtc gtcatcctga tggctaagct gctgcatttg tgctaaacgc tcggcttcca
    39001 tacgacgctg ttgttccagc tcctcaacct cttcaggcat acgtacctga actttgctca
    39061 gcgtactgat aacttcatat ttcaacgact ccagcatcgc tgcaaacatg gagaacgatt
    39121 cacgtttgta ttcctgcttc ggatctttct gtgcatagcc acgcaggtgg ataccctgac
    39181 gcagatagtc catcgctgcc aggtgctctt tccacaggga gtcaagcgtt tgcagcatga
    39241 cgcctttctc gaagtgacgc atcatctcag caccaaccac ttcttcttta cgctgataca
    39301 cttcgatgga ctgcgccaga atgcgctcac gcagcgtctc ttcatgcagt tctggttctt
    39361 tatccagcca ctcggcaatt ggtaaatcga ggtcgaaatc gttcttcaga cgttcctgca
    39421 gccccggaat atcccacatt tcttccagcg actgtggcgg aatgtaggca tcaatggtgg
    39481 ctttgaacac atcttcacga atgctgttga tggtttcgct cacatcgctg acatccagca
    39541 gttcgttacg ctgggagtaa atggcgcgac gctgatcgtt agccacgtca tcatattcca
    39601 gcagttgctt acgaatgtcg aagttacgac tttcaacttt acgctgggcg ttggcaatcg
    39661 ctttagtcac ccacgggtgt tcaatggctt cgcctagctt catacccagt ttacgcatca
    39721 tgccggatac tcggtcggaa gcaaaaatac gcatcagcgc atcttccatc gacaggtaga
    39781 aacgggaaga accagcatcc ccctgacgac cagaacgacc gcgcaactgg ttatcgatac
    39841 gacgggattc gtgacgctcg gtaccgatga tatgcaggcc acctgcttcc agtaccgcat
    39901 cgtgacgtac ctgccagtcg gctttaattt tttcaatttg ctctgcggtc ggattttcca
    39961 gcgcggcaac ttctgcctgc cagctacctc cgagcacaat atctgtacca cgacccgcca
    40021 tgttggtcgc gatagtcacc gcagccggat aacctgcctg agcaacaatc gccgcttcgt
    40081 tggcgtggaa tttggcgttc aggacattgt gcttaatacc ggctttggtc agttcgtttg
    40141 acaccagctc cgatttttcg atggagatag tacccaccag caccggctgg cctttcgcag
    40201 tacgttcttt gatatcttca atgatcgcct gaattttttc cgcttcagtc atgtagacca
    40261 ggtccggcag atctttacga atcattggac ggttggtcgg aacaacgacg gtatccagtt
    40321 tgtagatgga gctaaattcg aaagcttcgg tatcagcagt accggtcatc cccgccagtt
    40381 tttcatacag acggaagtag ttctggaagg tgatcgaagc cagcgtttgg ttttcgttct
    40441 ggatctgcac accttctttc gcttccacag cctggtgcag accatcggac cagcgtcggc
    40501 cctgcatggt acgaccggtg tgttcgtcaa cgatgataac ttcaccatct ttaacgatgt
    40561 agtcgacgtc acgggtaaac agcgcatgag cgcgcagcgc cgccgttacg tggtgcatca
    40621 gcatgatgtt ggccggagag tacagagact ccccttcatc catgatgccc tctttcacca
    40681 gcagttcttc aatcagcacc agaccacgtt cggtcaggtt cacctggcga gatttttcgt
    40741 ccaccgagaa gtggccttcg ccctggaagg tttcggagtc ttctttttcc tgacggatca
    40801 ggtgcggaat aattttattc acgcgtttat acatttccga gctgtcttct gccgggccgg
    40861 aaatgatcag cggtgtacgc gcttcatcga tcaggatgga gtccacttcg tccaccagcg
    40921 catagtgcag tttacgctgt acacgttctt cagggctgaa cgccatgttg tcgcgcaggt
    40981 agtcaaagcc gtattcgttg ttcgtaccgt aagtgatgtc agctgcgtaa gcttcgcgct
    41041 ttgccggtgc tggcatgccc ggcaggttga taccaacagt caggccaagg aattcaaaca
    41101 gcggacggtt gttttcggcg tcacgttgcg ccaggtagtc gttgacggta actacgtgca
    41161 cgcctttacc ggttagtgcg ttcaggtaag caggcagcgt tgcggtcagg gttttacctt
    41221 caccggtacg catttcggcg atgcagcgtt cgttaagaac cataccgccg agtaactgaa
    41281 cgtcgaagtg acgcatacca aagacgcgct tacttgcctc acgcaccacg gcgaaagctt
    41341 ccgggatcag attttccagc acttcgcctt tttccagacg cgcacgaaac tctgcggttt
    41401 tccctttcag ttcttcatcg gagagttttt ccatctccgg ttccatggca ttgatgatgt
    41461 tgaccacttt gcgcatccgg cgcagggtgc gatcgttacg actaccgaaa actttagtta
    41521 acaatttgat tagcataata aaatctcaaa cgccccgcgt tacggagtta ataaaatgaa
    41581 gtaaaggttt attgttgtta ggtgaggcgt tgagggccag cacggatgcc ttgcgcctgg
    41641 cttatccaga cgggcgtgct gaattttgct tgtggggtaa aatgcgcata atcaatgcga
    41701 taaccctttt cagacggcgt gccttcctgg gtcagcagcg cgctgagcgt atccagtaat
    41761 gcaagatgtt gcgcctgaag aggcaaagat tcttcagcaa cgggcagtgt ttgcggtgcc
    41821 attgcgaaag aaagatgacg gattaccgtg cgaatagcat gttgatgcca gtaatcaacg
    41881 gaatagttcg aattcgggcg gcgcgtgttc gcttccagca aggccaattg accaaagtta
    41941 actttggctg aaggctcgtg gttgcgggtt gtcgcttttg cgggcgcgtt tggttcggcg
    42001 gcgttgctga gcgcaggcaa acctaaactc gccgcgacca tccctaataa gagatgcggc
    42061 cagaagtagc gtttaccaaa ctgtcgccag cgcgtcagta ttccactcac gttattgcca
    42121 tcccgatcga attcaaacgt atttagcgca caaatcttat cattaaagcc tgaagtccac
    42181 agcacgaatg aagataagtt gtgcgtaaaa gtgcttaaga aaacagcgtt cgcaccgttt
    42241 tttatagctc ttataaaatt caaaaaaaaa cgactggttc acctggccgg agagagtgcc
    42301 agatttgcca gtcgaatttt atacgacagt ataaatgtcg ttatgccagt acagctgaag
    42361 gcgctttgaa ggccaacggc agttctgcgt cgtcctggaa ggtcacatat tcccaggctt
    42421 cctgtttcgc caggacagcc tgcagcagtt tgttattcag tgcatgaccg gatttataag
    42481 cggtaaatgc accaataata ttgtgaccac acatgaacaa gtcaccgatc gcatcgagca
    42541 ttttgtgacg cacaaattcg tcttcaaaac gcaggccgtc ttcgttcagt acgcgataat
    42601 cgtcaacaac gatggcacaa tcgaagctgc cgcccaggca caaaccacgg gactgcagat
    42661 attcgatatc acgcatgaaa ccgaacgtac gcgcacggct gatctggcgc ataaacgcat
    42721 cagcggagaa gttcatcgca tagcgctggt tgctggaatc aatagccgga tggttaaaat
    42781 cgatggtgaa atccagcgaa aaaccattgt acggcttaaa ttcagcccac ttatcgccat
    42841 cttcgacacg aacagtctct ttgatgcgaa caaatttttt ggcgcagttc aactcgtcga
    42901 taccggcgtc aagcagcagg tatacaaacg gagcggcgct gccgtccatg atcgggattt
    42961 ccggcgcgtt aacttcgata acaatgttat cgatgcccag gcccgcgaga gcagcattga
    43021 ggtgctctac ggttgaaatc cgtacatcat gctcgttgac cagacacgta cagagcatgg
    43081 tatcacgcac agatttggca tcggccggga aatctaccgg tggattcaag tcggtgcgac
    43141 gatagatgac cccggtgttg gccggcgcag ggcgtaacgt cagggtgact ttcttgccgg
    43201 tatgtaaacc gacacccgtc gcctgaacga tacgtttaag tgtcctttgt ttgatcatcg
    43261 tattatctcg ccaaattacc tatccaaccg aagtgtacta tacattcggc gggccagttt
    43321 agcacaaaga gcctcgaaac ccaaattcca gtcaattctt aatcagcttg cttacgtagg
    43381 aatgctggga tatccagata atccggctct ttcgcagttt gcggcgcatt gtcattcact
    43441 actttagcaa ccggcttctg ctcctgggtc agcggagcca tcccatgctg ctggtagcga
    43501 tccatcactg gctgctgaac ctgcttattg gtcaccagag tgatttcagg acgtttgtcc
    43561 atgccgatac ctgtcgcaac aacggttacg cgcagctcgt cattcatatc cgggtcaaga
    43621 gaagtaccga taaccacagt cgcgttgtcg gaagcaaatg cacggatggt gttacctacc
    43681 gtttcgaact catccagacg caggtcgaag cccgccgtga tgttaaccag cacgccgcgc
    43741 gcgccagaca ggtcgatatc ttccagcagc ggagaagaga tagccatttc agcagcttct
    43801 tccgcacggt cttcaccgct cgccacgcca gaacccatca ttgcgtagcc catttcagac
    43861 attacggtgc gtacgtctgc aaagtccacg ttcatcaaac ccggacgagt aatcagttcg
    43921 gcgatacctt gcacagcgcc tttcagtaca tcgttcgctg cgccaaacgc atccagcagg
    43981 gagataccgc ggcccagaac tttcagcagt ttgtcgttcg ggatagtgat cagagagtcc
    44041 acatgcttgg acagttcagt gatcccctgc tcggcgaatg ccatacgctt cttgccttca
    44101 aagttgaaag gcttagtgac gacagcaacg gtcaggatac ccaaatcttt tgccacttca
    44161 gcgacgactg gtgctgcacc tgtaccggta ccaccaccca tacccgcagc aataaagacc
    44221 atgtctgcac cttccagcgc cgcacgcaat gcatcgcgat cctcatcagc cgcattgcgg
    44281 ccaacttctg gattagcgcc agcgcccagt cctttggtga taccgctacc gatttgaatc
    44341 gtctgtccaa ccgctgtttt acgcagcgct tgtgcatcgg tatttaccgc gaagaattca
    44401 acaccttcaa tacgctcgcg caccatgtgt tcaacagcat taccgccgcc gccgccgacg
    44461 ccgatgactt taatcaccgc gtcattggta agttccattg gttcaaacat agtttctctc
    44521 cgatttgtgc ctgtcgcctg aggccgtaat catcgtcggc ctcataaaaa ttaaaactct
    44581 tttcgcagcc aactattgag tcgcttgatc cacgagccaa ctgatgctgt aacacgtttt
    44641 tctacttcag cttcaccgtt aagatgtgac tctttcccat agtgaagcaa tcccaccgcc
    44701 gtcgaataat acggctcctg agcataatcc gttaaaccgg taatgttcag cggcgcgccg
    44761 atacgcactt gcgtatgaaa cacgcgctga gcacaggctg caagaccttc gatctgcgct
    44821 gcgccaccgg ttaatacaat gcctgccgcc aggtgatgtt taaccccttg ttggcgaagc
    44881 ttttcctgca actgcaatat ctcttcgttg accaggttga gcaactcggt atagcgcggc
    44941 tcgatcacct ctgccagggt ctgacgttgc agactccgcg gcggacgacc gcctacgctc
    45001 ggcacttcca cgctctcatc ttttccaacg atggaaccca gcgcacaacc gtggcgaact
    45061 ttaatcgctt cggcgtcgct tggcggcgtg ccaaaggcgt aagcgatatc actggtcacg
    45121 acattgccag cataaggaat taccttagtg tggcgtaatg ccccaccggt ataaacggcg
    45181 atatccattg taccaccacc gatatcgacg acgcagacac ccagttcacg ttcatcttcc
    45241 gtcaataccg aataacttga tgccagtccg gcaaatatca gttggtcaac tttcagccca
    45301 caacgttcaa ccgctttgac gatgtttttc gccatatcgt tgtgacatgt gatcaggtgc
    45361 acttttgcct gcatccgcac acccgaaagt cctaccggat tcttgatccc ttcctgatag
    45421 tcaatcgcat actcttgcgg gatcacatgc agcacacgat gctcatcgcg cacacgcacc
    45481 gatttcgcgg tatggacgac gttttccaca tcttcttgcg tcacttcttc ttcagaaata
    45541 ggcaccatac caatttcatt ctggcagctg atgtgcttac cagaaagcgc cagatatacc
    45601 gaagagatct gacaatctgc catcaattct gcctggtcaa tggcgcgttg tacgcacttg
    45661 accacggatt cgaggtcgtt caccccgcct ttatccatac cacgtgacgg gcagctgccc
    45721 acgccaatga tattgaccat accgtcgggc agaacttccc ctactaaagc ggcaaccttc
    45781 gcggtaccaa tttccagtcc tactaccagt tttctgtccg tcgccttgat cattgttgtt
    45841 ctgcctgtgc ctgattttgt tgctgagtag attcctctgg cggcaagggc gcccagccta
    45901 ctgccgctcc agagtcataa cgcaaatcaa cgtagctaat ccgtttgcca tcggtttgcg
    45961 cctgctgctg taaaaccgga taaagttcta caaagcgagc caaacgtttc atcgtatcgc
    46021 cccggccaag attgagctta atatcgttat tcagcgtcaa ctgccaggaa cgccgcgcgg
    46081 tcatcgccgc ttccttcaga gtaaatctgt cctttgccag catctgcccc atttcgcgat
    46141 agccctgcaa cacttcatta gcgctgcctt ccgggccata cagcattgga agcacctgct
    46201 tgctggtgcg atctggcggc acgctgaagg tgtttccttc cgcgtctacc atgtgttgat
    46261 cattccaccg cgcaatcggc acatattcaa ccagatgaat cttcaattca tcaggccact
    46321 gctttctgac gctcacctgc ttaatccacg gcaggcgttg ttctatttgc gtctggatga
    46381 tgttgacatc ctgggtcata aaggtacccg gctcacccaa tgccaggatc gactgccgga
    46441 tatcgtcatt acgcgtgtaa tggcgttcac cggtcaacac cagctttgag agcggcaggc
    46501 gttgcgcatc ttccatccag ctcaacacga cccagccgct caccaacact gtcgttaaaa
    46561 cggtcagcag gaaaaggatc cccgccagac gcgttccatt attgcggcga gaagaaacct
    46621 cttcttcgct gtttcgcgtg ttcagagcag cctgcgacat attagtccgc cagttccaga
    46681 attcgtacta ccaactgcga gaagctcata cctgcctgac gtgccgccat cggcaccagg
    46741 ctgtggctgg tcatacccgg tgaggtattg gcttccagca gataaaactg tccatcactg
    46801 tccagcataa cgtcaatacg tccccatcct ttgcaaccta acgtcgtcca tgctttcagc
    46861 actaatgcct gcaaattggc ctcttgtgac gctttcagac ctgcggggca gaaatactgt
    46921 gtctcatcag agagatactt cgcctcataa tcatagaagg ttccggacgg ttgaatacgt
    46981 attgacggta aaatttcttc accgagtatc gcaaccgtga actccggccc acttagccat
    47041 ttttcaatca atacttcttc atcgtgctga aatgccaatc ttaatgcatc ttgtagagca
    47101 ttttctgcta ctacttttga cattcccaca ctggaacctt cgcggctcgg cttaacgata
    47161 accggcaaac ccagagcaga aatttctgct aactgcttat cgctcaggcc tttttcaaac
    47221 tctgcgcggg ttaacgctac ccacggcgcg accggtaaac cggcaccttg ccacagaagt
    47281 ttgctgcgta gtttatctat tgaaagcgca gatgccatca ctccgctgcc ggtataaggc
    47341 aagcccatca gctcgagcat gccctgcagc gtaccatctt caccgccgcg accgtgtagc
    47401 gcgataaaca ctttctgaaa acccatcgac ttcagttgcg tcacgtcgac ttctttcggg
    47461 tcgacaggat acgcatcaat accgccttca cgcagcccgg ctaacaccgc tgcgccagaa
    47521 ttcagagaaa cttcccgctc agcggaagtc ccgcccaaca ggaccgcgat tttatcagtc
    47581 atgttgttct tcctccggag tttgcggctt cagtttgatt tcagctaaag aacgggcaat
    47641 ttttccgata ttaccagccc cctgaacgag aatcaggtcg ttaccggtta ataccggtgc
    47701 cagcatctcg gctacctgcg cagggtccgg caccagaatg ggatcaattt tcccacgtcc
    47761 acgaattgtg cgacacagcg aacggctgtc cgctcccgga attggcgctt cgccagccgg
    47821 atacacttcc agcatcaaca gggtatcaac ctgcgtcagc acattggcga aatcatcata
    47881 caggtcgcgc gtacgggtaa aacggtgcgg ctgaaacagc attaccaggt ttttatccgg
    47941 ccagcctgcg cgcgccgctt taatggtggc gtcaacttcc gtcgggtggt ggccgtagtc
    48001 atcgaccagc attgccgtac cgcttttacc attcactggc tccagcggga attcaccgag
    48061 gaaatcaaaa cggcgaccag tcccctggaa gctctccagc gcacgcaaaa tagcctcgtc
    48121 gtcaattccc tcttccgtag caaccgcaac cgcagctgcg gcgttcagcg cgttatgacg
    48181 gcctggcgca ttcagggtga cgcgcatcgg ctctttgtcc tgacgcagca gcgtaaagtg
    48241 cccctgcggg ccaatctgct gataatcttc tacacgcacg tcggcatctt cgctgaagcc
    48301 gtaagttgtg gtctgacgtc ccacacgcgg taacaattcg cggatcaccg gatcatcaac
    48361 acacatcacc gcacgaccgt aaaaaggcag gttgtgcaga aaattaataa aggtctgttt
    48421 taaattctca aagtcgccct ggtaggtatc catgtggtcg gcttcgatat tggtgacaat
    48481 cgccaccatc ggttgcagat gcaggaacga tgcatcactc tcatctgctt cggcaatcag
    48541 gtaccgacca tgccccaaac gcgcatgaac ccccgccgct tttaccagcc cgccgttaac
    48601 gaaggttggg tcgagccccg cttctgcgta gatgctggaa accatcgcgg tagttgtcgt
    48661 tttgccgtgc gttcctgcaa tggcgatgcc atgacgaaaa cgcattaact cagccagcat
    48721 ttcggcacga cggatcaccg gaatacgcgc ttcatgagct gcgacaattt ccgggttgtc
    48781 ggcagaaatg gcgctggaaa caacgaccac gctggcatca cgtacgtttt ccgggcgatg
    48841 gttgaaataa atcgtcgcac ccagattcag taactgctgc gtgaccggat ttggcgctaa
    48901 atcggaacca ctgatctgat aaccttcatt ggccagaact tcggcaatac cgcccatacc
    48961 ggcaccacca atgccgacaa aatgtatgtg ccgaacgcga cgcatttcgg gcacgatgga
    49021 acgcagtttt gccaattgtt gtgtattcat tctttacgcc attaacttct taaattcata
    49081 cgatgcaaaa ggcatcgcta caattacgcc cgggcagccc ggctcacttc atttgccact
    49141 cgctcggtgg catccggaat ggatgcagcg cgggcgcgtt ctgccatggt taataaggtt
    49201 tctcgcgacc acccggccag ggtgttggcg acagcatcca cgctaagctg tggctgctcg
    49261 ataattttgg ctgcgcccgc tttttccagc ggtagcgcat tccagtattg ctggcggtct
    49321 ttatgttgaa acggcacaaa caacgccggt agtcctgccg cggcgatttc actcacagtt
    49381 aaagcaccgg agcggcaaac gacgacatcc gcccacgcat acgccgccgc catatcatca
    49441 ataaattcag tcactttatg ctgcggttgc cccgcttcgg catacgcctg ttcaacggat
    49501 tgttgcgaac ctttgccgct ctgatgccag atagtgactg aatcacccag tttcgcagca
    49561 acctgcggca ttgtctggtt aagaatgcgt gcgccctggg aaccacccac taccaacaca
    49621 cgaaccggac cttcacgtcc agccaaacgt tgctgcggca acggcagcgc cagcacatcg
    49681 gtacgcaccg ggttacccac cacttccgca ttagggaaag cgcctggaaa cgcctgcatc
    49741 actttggtag caatcttcgc cagccattta ttggttaagc ccgcaatacc gttttgttca
    49801 tgaagtacaa ccggaatgcc taacgaccac gcggccagac cacctggacc tgatacatag
    49861 ccgcccatac cgagtaccac gtcaggtttg tacgctttca taatcgcccg cgcctgacgc
    49921 cagccgttga agatacgcag cggcgcagct atcagtgctt ttataccttt tccacgcaaa
    49981 ccagagatac gaatgaaatc aatttcgatg ccatgttttg gcactaagtc cgcttccata
    50041 cggtcggcag tccccagcca gcgaacttgc caaccctgag ccattagata gtgcgcaacc
    50101 gccagtcccg ggaatacatg tccaccggtt ccgcctgcca tcaccattaa tcgctttcct
    50161 tgaccactca tcgtgaacct cgtacaaacg cctgcgcttt ctccagacgc gtttcataat
    50221 caatacgcaa cagcatcatg atggctgtcg acataatcag taagctcgaa ccaccgtaac
    50281 tgattagcgg caatgtcaga cctttggtcg gtaacatccc cgccgccgcg cctacgttaa
    50341 ccagcgcctg gaagctaaac cagatgccaa tagaacaggc gagaaaaccg gaaaaacggt
    50401 ggtcaatttc taatgctttg cggccaatcg acatcgcacg aaaagcgacg aagaatacca
    50461 ttaaaagtgc tagtaccaca ccgacataac ctaattcttc gccgataatg gcgaaaataa
    50521 agtcagtgtg cgcttccggc agatactcca gtttttgtac cgaattacct aaaccttgcc
    50581 cccaaagttc gccgcgacca aacgccatca gcgattgcgt taactgatag ccgctgccaa
    50641 agggatcttc ccacgggttc cagaatgcgg taacacggcg gatacggtac ggttcggcga
    50701 ggatcagcaa cacaaccgct gaaatgccca taccgataat ggcaatgaac tgccacaatt
    50761 tcgctcccgc caggaacaac atcgccagcg tagtcacaaa caacaccacc accgtaccaa
    50821 ggtctggctg tgccagcagt aacactgcca acaccagaat cacgcccatc ggtttcagga
    50881 agccgcgcag gttattacgt acttcgtcgc ctttacgcac cagatagttg gcgatatagc
    50941 aaaacagcga cagttttgtc agctccgcag gctggatacg cagcaaaccg agatcgatcc
    51001 aacgcgatgc ccctttaaca gcgctaccta ctaccagcac gatcatcagc aggatgatag
    51061 atccgagcag catcgtggca ctgtagcgtt gccagaactc catcggcaga cgcagcgtaa
    51121 tgatcgccag aataaacgcc aaaatcagat agacaccatc acgcttcgcg aagaagaacg
    51181 gatcgttggt taagcgttgc cctatgggca ttgacgccga ggtcaccatg ataaagccaa
    51241 tcgccgcgag gccgaaggtc agccacagta aggtgcgatc gtacatgatc aggctgtcgg
    51301 tatctttttc ccgcgagccc atcacccagc cctttagcgc cgtggagatc cagaccaaga
    51361 tactgaatcc tggcaggcgc ggcattttca ggcgagggag agataaacgc atcaacctaa
    51421 ctccttcgcc agacgggcaa actcattgcc tcgttgttca aagttcttga actgatcaag
    51481 gctggcacag gccggggaga gcagaaccat atcgcccggc tgaacacgcg gagccagcaa
    51541 gcgcatagcc tgttccatag tttcggtttg ttctgccact tccgggcgta gcgccgccag
    51601 ctgcgcgccg tcacgaccga aacaatacag acgtacgtta tcgccattca ggtaacgcgc
    51661 cagtgggcta aagtccgccg atttaccatc gccgcccagc aacaaatgca gcgtgccgtc
    51721 tacgtgcagg ccattcagcg ccgcttccgt actgccgacg ttggtcgctt ttgaatcgtt
    51781 aatccagcgc acaccgttat gctccagcac cacttcaaag cgatgcggca gaccggtgaa
    51841 ggtggttaac gctttcaggc tgctggcgcg cggtaaccct gcggcatctg ccagcgccag
    51901 cgccgccagc gcattggtgt agttatgctg cccggaaagt ttcatctctt tcacattcag
    51961 cactttctcg cctttaaccc gcagccaggt ttcgccctgc tgatgattca ggtgatagtc
    52021 acccatgttg acgccaaagc tgacgcagcg ttcatccgca ccgcgaatcg gcattgttaa
    52081 ggcatcatca gcattaacca cgcaaacttt cgcgttttcg taaatgcgca gttttgctgc
    52141 acgatactgt tgtaaaccaa acgggtagcg atccatatga tcttcagtca cgttcagaat
    52201 ggtcgctgct accgcctgta agctggaggt ggtttccagc tggaagctcg acagttccag
    52261 cacgtacagt tcacactcat catccagtag catcaacgca ggcaggccaa tattgccacc
    52321 cacaccaacg ttaacccccg ccgctttcgc catttcaccc actagcgtgg tgaccgtgct
    52381 tttgccgtta gaaccggtaa tcgccacaat cggtgcttgt gcttcgcgac aaaacagctc
    52441 gatatcgcca acgatttcga ttccggcatc agcggcagcg cttaaggatg gatgcgccag
    52501 tgcaataccg ggactggcga caatcagatc tgccgccatc aaccattcat cattcagact
    52561 gcccgtgtgg cgttctacgg cttcgggtaa tttatccagg ctaggcggtg tcatacgcgt
    52621 atccataacg cgcggcgtca caccgcgagc gaggaaaaag tccacgcagg aaagcccggt
    52681 gaggcccagg ccgataatga caacattttt accctggtaa tcagccatga ttaacgtacc
    52741 ttcagcgttg ccagaccaat caggaccagc atcagcgaaa taatccagaa acgcacaatg
    52801 acgcgcggtt ccggccagcc tttcagttca tagtggtgat gaatcggtgc catgcggaaa
    52861 atacgttgtc cgcgcagttt aaaggagccg acctgcagga tgacagaaag cgtttctacc
    52921 acgaacacgc cccccataat caccagcagg aattcctgac gtagcagtac ggcgataatg
    52981 cctaacgcac cacctaacgc cagcgaaccg acatcgccca taaagacctg cgccggatag
    53041 gtgttaaacc acaggaagcc cagtcctgcc ccgactatcg cggtacagac aataaccagt
    53101 tccccggcgt gtcgcagata cggtatgtgt aaatagctgg caaagttcat gttgccggtc
    53161 gcccacgcca ccagcgcaaa accaccggcg acaaatacgg tcggcataat tgccagaccg
    53221 tcaagaccat cggtcaggtt taccgcgttg ccagtaccca caatgacgaa gtaagccagc
    53281 agaatgtaga acagccccag ttgcggcatc acatctttaa agaatgggac caccagctgc
    53341 gttgcgggcg tgtctttgcc ggcaaggtac aaagcgaaag caacacccag cgcaatgacc
    53401 gacatccaga aatatttcca acgagcgatc aaccctttgg tatctttacg caccactttg
    53461 cgataatcat caacaaagcc aataacaccg taacctacca gcaccaccaa cacgcaccag
    53521 acgtacggat tggacgggta agcccacagc agcacggaga tcacaatcgc tgtcaggatc
    53581 ataatcccgc ccatggtcgg cgtaccgcgc ttgctgaagt gtgattcagg accgtcgtta
    53641 cgcactacct gaccaaagga aagtttttgc aaatgagcaa tcatgcgcgg gcccatccac
    53701 aatgagatga acagcgcggt cagcaggctg acgatggcgc gaaacgtcag ataggaaaag
    53761 acgttaaagc cggaataata tttgaccaaa tgttcggcca gccaaactaa catgtcccat
    53821 tctcctgtaa agcgcgtact acctcttcca tggcagcact acgtgaaccc ttaactaaaa
    53881 tcgtaattac ctgttgctca gcaatcagtg atttaagacg cgtaattaac gcagttttat
    53941 cagcaaaatg ttcgccaacg ccgctggcgg tgctgatagc atggctttgt ttacccatgc
    54001 ttaacacgcg gtcaatacca gcagcttttg ccgcctcgcc cacctgtaca tggcagtctt
    54061 cgctttcagc gcccagttcc gccatatcgc ccaccaccag cacgcggtag cccggcattt
    54121 cagccagtac ctgaacagct gcggtcattg aaccgacatt ggcgttgtag gagtcgtcga
    54181 gcagcaactg gttttctgcc agttggatgg ggaacagacg gccaggaaca gctttcagat
    54241 ttgccagccc cgctttgata gcatcaagcg ttgcgcccac ggacatggag agcgcagcgg
    54301 ctgccagcgc attcgcaata ttgtgacgcc ccggcaacgg cagcagaaca tcgacgctac
    54361 cggttggggt ttgtagggta aattccatac cgtgcgaggt cacatggata ttggtggcgg
    54421 tgaaatcgct gttggcggca ttgggtgaga aacgccacac tttgcgtgag ccaattacgc
    54481 tctgccagtt cagccagtcg ttgttgtcgg cgttcataat ggcgataccg ttttccggca
    54541 ggccgctaaa gatttcacct ttcgctttcg cgacacccgc aagcgagcca aaaccttcca
    54601 gatgcgccgc tgccaggttg ttgaccagcg ccgcttccgg gcgagtcaga ctcacagtcc
    54661 tggcaatctc gccctgatgg ttcgcgccaa gttcaataac tgcgtaatcg tattccggcg
    54721 ttaagcgcaa cagcgtcatc ggtacaccga tgtcgttgtt gagattgcct gccgtataaa
    54781 gcgtgttgcc gcactggctt aaaatcgccg ccgtcatctc tttaacggag gttttgccgg
    54841 aggaccccgt cagagcaacc acgcgcgccg gaacttgctg gcgaacccat gcagccagtt
    54901 caccaaacgc cagacgcgta tccttgacga ttaactgcgg caggtcgatg tccagcggac
    54961 ggctaaccag tagtgcgcct gcgccgccag ctttcgcctg gtcggcaaaa tcgtgggcat
    55021 caaaacgttc acctttcagg gcaacaaaca ggcagcccgg cgtcagtttt cgggtatcag
    55081 tggttacagc atcaagggtg atatctgcac cttgcagttc accgttgaga atatcggtaa
    55141 gttggctaag ggttacgcta atcatgcaat cccccccagc agacgcgcca ccgtgacgcg
    55201 atcggagtag tccagacgct gattgccaac aatctggtaa tcctcatggc ctttgcccgc
    55261 gaccagtacc acatcattct ctttagcctg cataacggcg caagtcaccg cttcagcacg
    55321 gccttccatc actttggcat gtccggcatc taacattccc gccagaatat cgttgatgat
    55381 ggcacgcggt tcttcggtac gcgggttatc gtccgtcacc accgccacgt cagcaaactc
    55441 ttcggcaatt gcgcccatca gtggacgctt acctttatcg cgatcgccac cacagccaaa
    55501 gacgcaccac agcttgcccg cacagtgcag gcgcgccgcc tgtaaggctt tttccagtgc
    55561 atccggcgta tgcgcgtaat ccaccaccac cgtcggtttg cctggcgcag tgaacacttc
    55621 catacgtccg caaaccggtt gcagacgcgc ggcggttttc agcagatcag ccagtggata
    55681 gcccagcgcc agcagtgttg ccagcgcgag caacaggttg ctgacgttaa aagcgcccat
    55741 cagatggctt tcaatttcgc catcgcccca gcttgaacta aagcgaatgg tcgcgccgct
    55801 gtcgtgatag ttcacttcgg tcgctttcaa ccagcgtccg tgacagttcg gattgatatg
    55861 atcttccatt gataccgcaa ccgcgtccgg cagttttgcc agccagcggc ggcccacttc
    55921 atcgtcggcg ttaataatcg cctgaccgca atgatgttca gaataaagca gccatttcgc
    55981 agcttcgtag tgttccatat caccatgata atcaaggtga tcgcggctta agttggtaaa
    56041 gaccgacgcc ggaaatttca atgccgccac acggtgctgt accagcccgt gggaggaaac
    56101 ttccattgcg caaaaagtcg cgccctgatc caccagcccc gccagctcat gctgaacatc
    56161 aactgccgaa ccggtagtat tttctgtcgg gatcactttc cccagcagac cgttaccaac
    56221 ggtgcccatt accgcgctgg tttcgccaag cagttggctc cactgcgcca gaagctgggt
    56281 cgtcgtggtt ttgccgttgg tgcccgttac gcctacgaga cgtaaattgt cagagggttc
    56341 atggtaaaag cggcccgcca gtgcagataa acgctcgttg agctggctga gatagatgac
    56401 cggtacgccg tgcatttcac ggatttcacc gtcggtcgcc tcatctttcg cctctgcaat
    56461 aatggcagcc acaccttgcg ctatcgcctg cgggatatat cgacgcccgt ccgcctgatg
    56521 acctactaca gctacaaaga gatcgcccgc cgcagccaca cggctgtcga gtgtcatctc
    56581 tcgcagtgct cgcgaaggtg cgtctggcac ccacggagca agaaggtcgc gcaaattacg
    56641 atctgccacc tgtcccctcg ccttgattaa tcacaaattc atttttatcg cccgttgtca
    56701 gcgcatccgg ctcgatgttc atggtacgca atacgccgcc catgatggca ccaaagaccg
    56761 gcgcggaaac ggcgccgccg tagtatttac ccgcctgcgg atcgttgata acaacaacca
    56821 gcgcgaagcg cggctgactc gcaggcgcaa cgcctgcggt ataagcaata tatttattga
    56881 tgtagcgacc gtccggcccg acctttttcg cggtaccggt tttaatggcg atacgatagc
    56941 ctttaatcgc cgccttcacg ccgccgccgc ctggtagcgc cacgctttcc atcatatgca
    57001 ccacggtgcg aacaatggat tccgggaaga cacgttcacc gggaaccggg gggtcaactt
    57061 tggtaatcga cagtgggcga taaatgccgt agctgccgat agttgcgtag actcgcgcta
    57121 actgtaacgg tgttaccatt agcccgtagc cgaaagagaa ggtggccctc tctatgtcag
    57181 accaccgttg tttttgagga tataagccac tgcgttctcc gaccaacccc aaattggtcg
    57241 cttttcccag tccaaaacgt gagtaagtat ctactaacgc tgaggacggc atcgctaacg
    57301 ccagcttgga aacaccgacg ttactcgact tctgtaatac cccggtcagg gttaattcgc
    57361 tgtagcgtgc cacgtctttg atttcgtggc cgttaattcg ataaggaatg gtattgagta
    57421 ccgagttttc ccgcaccacg ccacgttgca acgcggtcat caccaccatc ggtttaaccg
    57481 ttgagcccgg ttcaaacacg tcggtgatgg tacggttacg catcgcctct ttcggcgtgc
    57541 cgctcagatt gttagggttg tatgacgggc tgttagccat cgccagcact tcacctgtgt
    57601 tgacatccac cagcacggcg ctaccagatt cagccttgtt aaaggccacc gcattgttca
    57661 gttcgcgata aaccagcgcc tgcaggcgtt catcaatact cagcgccagg ttgtgcgctg
    57721 cctggctgtc agtagaagaa atatcttcaa ttacgcgacc atagcggtct ttacgcacaa
    57781 tgcgctcacc cggctgcccg gtaagccatt tatcgaaact cttctcaacg ccctcaatcc
    57841 cttgactatc gacgttagta aagccgatga ggtgagcagt cacttcgccg gacggatagt
    57901 aacggcgaga ctcttcacgc agatgaatcc ccggcagttt cagttttttg atgtagtccg
    57961 ccatgtcaga gtttacctga cgcgccagat aaataaagcg ccctttcggg ttggcgttaa
    58021 tgcgggcaga aagctgatcc agcggaatat tgagcgcgtt agccagcgcc ttccagcggt
    58081 caccgacgct gataccgcca gcgtcatgca cttctttcgg gtcagcccaa atcgctttta
    58141 ccggcacgct caccgctaac gggcgaccag aacggtcagt aatcatgccg cgggaggtgg
    58201 aaacttgctg aacgcgaaga gaacgcatgt cgccctcttt caccagcata tccggggaga
    58261 taacttgtaa ccacgctacg cgtccgagca gaaaagccag cgccaggaga atacagccgc
    58321 ataacaacgc aaaacgccaa ctgataaagt tggcatgttc ttcctgacgt tttggtttct
    58381 gcgttttcgc cgctgctttc atgcgtcgcg tttatcctta tttttgcact acgatatttt
    58441 cttgtgacgg atcaacatgc tgcatttgca gcttttccgt ggcgatcctt tccacccggc
    58501 tatggtcgcc gagcgcattc tcttcaagga tcaggttgcg ccattcaatg tctaaagcat
    58561 ctcgctccag caccagttgt tcgcgctgag cggtcagtaa acgggtatgg tgcgccgtgg
    58621 ttaccacggt caccgccgtc aaaataatgc aaatgaacag gcagagtggc agcttcccaa
    58681 atcgcaaaag atcgtcaccg ataacaccag gcaatgcatg gcgctcgtgg cttcccatcg
    58741 atcctttaac tttgcttaga gcttctgtca ctctgctgat catgcgttcg tcctctctgc
    58801 aatacgcaga actgaactac gggcacgagg gttctcagcc acctcttctt cgcccggcat
    58861 taacttgcct agtgctcgca gctgacggcc acccagtttt ttgagctgct cttcagtcat
    58921 cggtaaccct gccggaactt gcggaccgcg gctgttttca cgcataaaac gtttcacaat
    58981 acggtcttcc agcgagtgga agctgatgat cgaaagccgc ccacccgggg ccagcacgtt
    59041 gagcgagctt tttagcgcct gctctatctc ctccagttca ctgtttaccc aaatgcgcac
    59101 agcctggaag gtacgggtcg cgggatgttt aaacttatct ttcaccggcg ttgcagcagc
    59161 cacgacttcc gccagttctt tggtgcgggt catcggctgt tcgcggttac gctcgacaat
    59221 ggcgcgggca atgcgtttgg caaaacgctc ttcaccatag gttttcaata cccaggcgat
    59281 atcggcttct tctgcggttt gtagccattc agcggctgac tgcccacggg ttgggtccat
    59341 acgcatgtcc agcggaccat cgcgcataaa ggaaaagccg cgttcagcat catcaagttg
    59401 cggtgaagag acgccaagat cgaggagaat gccgtcgatc ttgccaataa gatcgcgctc
    59461 ggcaacgtat tcgcccagcg cggagaaagg tccgtggatg atggagaagc gcggatcatc
    59521 aatagtcttc gcaacggcga tagcctgcgg gtcgcgatcg atcgccagca aacgcccctc
    59581 ttcgccaagc tgcgagagga tcagacgtga gtgaccaccg cgaccaaaag tcccatcaat
    59641 gtagatgcca tcaggacgga tattgaggcc attaacggct tcatccagca gcaccgtagt
    59701 atgtttatag ttttccatca ttttatagag acaagtcctg cagtcgctcc gataagtctc
    59761 cggtagccaa ctgctctgcg tcgatatctt ccttgacctg ttgatgccag gttgtttcat
    59821 cccacagctc aaacttgttg aactgtccaa ccagcatcac ttcttttgtc agcccggcat
    59881 gttgccgcag tactggcgcg attaacaatc gacctgcgcc atccatctga cattcgctgg
    59941 catgacctaa cagtaggcgc tgcacacggc gctcaaccgg gttcatgctc gacagacgcg
    60001 ataatttttg ctcgataatt tcccattcag gcagggggta aagcagcagg cacgggtgat
    60061 gaatgtcaat ggtgcaaacc atttgaccgg cagcgttctc aagcagctgt tcccgataac
    60121 gggtaggcac tgataagcgc cctttgctgt cgagattgac taacgttgct ccccggaaca
    60181 tgccagcctc accccttatt cccactttgc cccacaattt cccactgaaa ggagtttacg
    60241 gagcggagga aaagcttgtc aagccagtca caaggcatac cgaggtaaaa aacccgatgt
    60301 ttacggctaa tatcagcgtt gtttatttta cgtccaacgt gcgaagcaaa ttaacgttat
    60361 gaatagctgt aagaaaaaat ccctcatcgt catcactgtt ttaaaatgac tcactatttt
    60421 ccacagcaaa tataaagtgt cagtttgcga cgcgagcggc attttaggac atatcttccc
    60481 cggttaacag tccttgttac gtctgtgtgg cgcttgatgc cagcgggttt gcgcaaaagc
    60541 aaagaaaagt gtttgttaaa tcggcaattc atcgccgcat gtaacaaaat aatgcaaaaa
    60601 cgcgctgtcg atatcactta attattgtga atttcagctg gtgagttaat taacgggcgg
    60661 gtttaaaacc atttatcaat aatagtcagg aatagtctta tttactttaa ggatattgat
//
LOCUS       DAJVQH010000011        56563 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJVQH010000011 DAJVQH010000000
VERSION     DAJVQH010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.011"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..1007)
                     /gene="setA"
                     /locus_tag="OQM87_000782"
     CDS             complement(<1..1007)
                     /gene="setA"
                     /locus_tag="OQM87_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCS1507028.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     G"
     gene            complement(1109..1240)
                     /gene="sgrT"
                     /locus_tag="OQM87_000783"
     CDS             complement(1109..1240)
                     /gene="sgrT"
                     /locus_tag="OQM87_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCS1507029.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1329..2984
                     /gene="sgrR"
                     /locus_tag="OQM87_000784"
     CDS             1329..2984
                     /gene="sgrR"
                     /locus_tag="OQM87_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCS1507030.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3018..3117
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3148..4131
                     /gene="thiB"
                     /locus_tag="OQM87_000785"
     CDS             3148..4131
                     /gene="thiB"
                     /locus_tag="OQM87_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCS1507031.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPSGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4107..5717
                     /gene="thiP"
                     /locus_tag="OQM87_000786"
     CDS             4107..5717
                     /gene="thiP"
                     /locus_tag="OQM87_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCS1507032.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5701..6399
                     /gene="thiQ"
                     /locus_tag="OQM87_000787"
     CDS             5701..6399
                     /gene="thiQ"
                     /locus_tag="OQM87_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCS1507033.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(6513..7277)
                     /locus_tag="OQM87_000788"
     CDS             complement(6513..7277)
                     /locus_tag="OQM87_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1507034.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7363..8241)
                     /gene="araC"
                     /locus_tag="OQM87_000789"
     CDS             complement(7363..8241)
                     /gene="araC"
                     /locus_tag="OQM87_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCS1507035.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8580..10216
                     /gene="araB"
                     /locus_tag="OQM87_000790"
                     /pseudo
     CDS             8580..10216
                     /gene="araB"
                     /locus_tag="OQM87_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            10227..11729
                     /gene="araA"
                     /locus_tag="OQM87_000791"
     CDS             10227..11729
                     /gene="araA"
                     /locus_tag="OQM87_000791"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCS1507036.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            11929..12624
                     /gene="araD"
                     /locus_tag="OQM87_000792"
     CDS             11929..12624
                     /gene="araD"
                     /locus_tag="OQM87_000792"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCS1507037.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12699..15050
                     /gene="polB"
                     /locus_tag="OQM87_000793"
     CDS             12699..15050
                     /gene="polB"
                     /locus_tag="OQM87_000793"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCS1507038.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHNPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15214..18120
                     /gene="rapA"
                     /locus_tag="OQM87_000794"
     CDS             15214..18120
                     /gene="rapA"
                     /locus_tag="OQM87_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCS1507039.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAILLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18132..18791
                     /gene="rluA"
                     /locus_tag="OQM87_000795"
     CDS             18132..18791
                     /gene="rluA"
                     /locus_tag="OQM87_000795"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCS1507040.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(18908..19723)
                     /gene="djlA"
                     /locus_tag="OQM87_000796"
     CDS             complement(18908..19723)
                     /gene="djlA"
                     /locus_tag="OQM87_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCS1507041.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            19978..22332
                     /gene="lptD"
                     /locus_tag="OQM87_000797"
     CDS             19978..22332
                     /gene="lptD"
                     /locus_tag="OQM87_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCS1507042.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22385..23671
                     /gene="surA"
                     /locus_tag="OQM87_000798"
     CDS             22385..23671
                     /gene="surA"
                     /locus_tag="OQM87_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCS1507043.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRANAYVKILSN"
     gene            23671..24660
                     /gene="pdxA"
                     /locus_tag="OQM87_000799"
     CDS             23671..24660
                     /gene="pdxA"
                     /locus_tag="OQM87_000799"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCS1507044.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24657..25478
                     /gene="rsmA"
                     /locus_tag="OQM87_000800"
     CDS             24657..25478
                     /gene="rsmA"
                     /locus_tag="OQM87_000800"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCS1507045.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25481..25858
                     /gene="apaG"
                     /locus_tag="OQM87_000801"
     CDS             25481..25858
                     /gene="apaG"
                     /locus_tag="OQM87_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCS1507046.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25865..26707
                     /gene="apaH"
                     /locus_tag="OQM87_000802"
     CDS             25865..26707
                     /gene="apaH"
                     /locus_tag="OQM87_000802"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCS1507047.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26785..27264)
                     /gene="folA"
                     /locus_tag="OQM87_000803"
     CDS             complement(26785..27264)
                     /gene="folA"
                     /locus_tag="OQM87_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCS1507048.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27455..29317)
                     /gene="kefC"
                     /locus_tag="OQM87_000804"
     CDS             complement(27455..29317)
                     /gene="kefC"
                     /locus_tag="OQM87_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCS1507049.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29310..29840)
                     /gene="kefF"
                     /locus_tag="OQM87_000805"
     CDS             complement(29310..29840)
                     /gene="kefF"
                     /locus_tag="OQM87_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCS1507050.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(29888..31282)
                     /locus_tag="OQM87_000806"
     CDS             complement(29888..31282)
                     /locus_tag="OQM87_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1507051.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(31341..31628)
                     /gene="fixX"
                     /locus_tag="OQM87_000807"
     CDS             complement(31341..31628)
                     /gene="fixX"
                     /locus_tag="OQM87_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCS1507052.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31625..32911)
                     /locus_tag="OQM87_000808"
     CDS             complement(31625..32911)
                     /locus_tag="OQM87_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1507053.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSLSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESSPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(32962..33903)
                     /locus_tag="OQM87_000809"
     CDS             complement(32962..33903)
                     /locus_tag="OQM87_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS1507054.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(33918..34688)
                     /locus_tag="OQM87_000810"
     CDS             complement(33918..34688)
                     /locus_tag="OQM87_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCS1507055.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVNA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35159..36673
                     /gene="caiT"
                     /locus_tag="OQM87_000811"
     CDS             35159..36673
                     /gene="caiT"
                     /locus_tag="OQM87_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCS1507056.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFDIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36704..37846
                     /gene="caiA"
                     /locus_tag="OQM87_000812"
     CDS             36704..37846
                     /gene="caiA"
                     /locus_tag="OQM87_000812"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCS1507057.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            37975..39192
                     /gene="caiB"
                     /locus_tag="OQM87_000813"
     CDS             37975..39192
                     /gene="caiB"
                     /locus_tag="OQM87_000813"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCS1507058.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCAGGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            39266..40819
                     /gene="caiC"
                     /locus_tag="OQM87_000814"
     CDS             39266..40819
                     /gene="caiC"
                     /locus_tag="OQM87_000814"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCS1507059.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            40928..41713
                     /gene="caiD"
                     /locus_tag="OQM87_000815"
     CDS             40928..41713
                     /gene="caiD"
                     /locus_tag="OQM87_000815"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCS1507060.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            41719..42309
                     /gene="caiE"
                     /locus_tag="OQM87_000816"
     CDS             41719..42309
                     /gene="caiE"
                     /locus_tag="OQM87_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCS1507061.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(42395..42790)
                     /gene="caiF"
                     /locus_tag="OQM87_000817"
     CDS             complement(42395..42790)
                     /gene="caiF"
                     /locus_tag="OQM87_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCS1507062.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(43052..46273)
                     /gene="carB"
                     /locus_tag="OQM87_000818"
     CDS             complement(43052..46273)
                     /gene="carB"
                     /locus_tag="OQM87_000818"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCS1507063.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(46291..47439)
                     /gene="carA"
                     /locus_tag="OQM87_000819"
     CDS             complement(46291..47439)
                     /gene="carA"
                     /locus_tag="OQM87_000819"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCS1507064.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(47894..48715)
                     /gene="dapB"
                     /locus_tag="OQM87_000820"
     CDS             complement(47894..48715)
                     /gene="dapB"
                     /locus_tag="OQM87_000820"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCS1507065.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(48882..49796)
                     /gene="rihC"
                     /locus_tag="OQM87_000821"
     CDS             complement(48882..49796)
                     /gene="rihC"
                     /locus_tag="OQM87_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCS1507066.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(49862..50812)
                     /gene="ispH"
                     /locus_tag="OQM87_000822"
                     /gene_synonym="lytB"
     CDS             complement(49862..50812)
                     /gene="ispH"
                     /locus_tag="OQM87_000822"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCS1507067.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(50814..51263)
                     /gene="fkpB"
                     /locus_tag="OQM87_000823"
     CDS             complement(50814..51263)
                     /gene="fkpB"
                     /locus_tag="OQM87_000823"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1507068.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(51350..51844)
                     /gene="lspA"
                     /locus_tag="OQM87_000824"
     CDS             complement(51350..51844)
                     /gene="lspA"
                     /locus_tag="OQM87_000824"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCS1507069.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(51844..54660)
                     /gene="ileS"
                     /locus_tag="OQM87_000825"
     CDS             complement(51844..54660)
                     /gene="ileS"
                     /locus_tag="OQM87_000825"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCS1507070.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(54703..55644)
                     /gene="ribF"
                     /locus_tag="OQM87_000826"
     CDS             complement(54703..55644)
                     /gene="ribF"
                     /locus_tag="OQM87_000826"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCS1507071.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(55652..55870)
                     /locus_tag="OQM87_000827"
     CDS             complement(55652..55870)
                     /locus_tag="OQM87_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCS1507072.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            55973..56236
                     /gene="rpsT"
                     /locus_tag="OQM87_000828"
     CDS             55973..56236
                     /gene="rpsT"
                     /locus_tag="OQM87_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCS1507073.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        13544 a        15490 c        14680 g        12849 t
ORIGIN      
        1 cttccaggca ttaaatcctg aaaccatagc atcccaatac ccgcaacaat gccgataaat
       61 acagcgttaa aaagttgcag cgtcatcaac gccatacggc tatgaaagaa aatcaatcca
      121 gtgtaaaaca gcactcctgc caccactgca atgaccatca ttcgccgctt accataacgt
      181 ttgacatagt agccagccag aatcattgct ggtatttcca gtccagctgc cgtccccatc
      241 aggaaacccg ccagtttgtc tggcaatcct aaatcgctac tgatccacaa cggcatatca
      301 ataatgtaca tagtgttgca ggtccacatt aacgtcgagg cgacaaataa catccgtacg
      361 ttactatcct gccagtcacc ttgcattgat aaagcatttt ccgacggcag ttctacccgc
      421 gccacagacg gaagcataaa tgcaatcaat accagactga gtgtgaatat cccggcggca
      481 atcgaaaaca tcactgtaaa gccgtaattc aacgccagca taaaggccag cggtggaccg
      541 attacccatg ccagagaaag ctgcgcacgc atcaccgagc taaacatcac cacttctcgc
      601 gccgagttat ccgcgtattc ccgcgccaga gcaaataact gtggcattgc cgtattggcc
      661 agagatgcca gaagcacacc acaggtgata agcgtcagat aatgacgatt aaatgcaaac
      721 agtagcgcat tgccaatagc catcaaacag caaaatataa tcagttttcg ccgatcgccc
      781 tgactgtcag aacgttttgc caaccagagg cttacgccga tcccagcaat agcattcacc
      841 gtataaaaga gtccgatcca gaaaggttgc gcgccaacct cacgactcag aaataagctc
      901 aatgtaggag cctgtagcgc cccggccacc cccatcataa aagcgaccag cataaatgcc
      961 gcgtaaacac cgttcatacg gcgagccatc gtcattatcc agatcatacg ttcccttttt
     1021 agcgcggcga gaataaaaaa aaccagcagg tataatctgc tggcgggtga ttttacacca
     1081 atactcagtc acacatgatg caggcaagtc aactttcaga attgcggtca tcccactgca
     1141 tcagtccttc aagcatggtt aatcgttgtg ggacgcttaa ccaacgcaac cagcacaact
     1201 tcgctgtcgc ggtaaaatag tgctgataaa actgacgcat ggggcatctc cttgcttcat
     1261 cgttaggaat tattgactta atatagggaa aataaaattg ctgtcttttg cgcaggagtt
     1321 ccccttttat gccatctgct cgtctgcaac aacagttcat ccgcctgtgg caatgctgcg
     1381 agggtaaatc gcaggacaca acgctcaacg aactggcagc gttattgagc tgctcgcgtc
     1441 gtcatatgcg taccctgctc aacaccatgc aggatcgcgg ctggctgacg tgggaagcgg
     1501 aagtcgggcg cggtaaacgc tcgcgtctga cattcctcta taccgggctg gcgcttcagc
     1561 aacagcgggc ggaagacctg ctggagcagg atcgtatcga ccaactagtg cagttggttg
     1621 gcgacaaagc gactgtgcgg caaatgctgg tttctcatct gggccgcagc ttccgccagg
     1681 ggcggcacat cctgcgcgtg ctctactatc gtccgttgcg taatctgcta cctggcagcg
     1741 cattgcgccg ttccgaaacc catattgccc ggcaaatctt cagttcgcta acgcgcataa
     1801 atgaggaaaa tggggaactg gaagcagaca tcgcccacca ctggcagcaa atatcaccgc
     1861 ttcactggcg tttctttttg cgtccaggag tccattttca tcatggtcgt gaactggaaa
     1921 tggacgacgt gatcgcctct ttaaaacgaa tcaatacgct gccgctctat tcgcatatta
     1981 cggacattgt gtcgccgacg ccctggacgc tggatatcca tctcacgcag ccggaccgct
     2041 ggttgccgtt actgctgggg caagttccgg cgatgatcct gccgtgcgaa tgggaaaccc
     2101 tcagtaactt tgccagccat cctattggca ccggtccgta tgcggtgatt cgcaacagca
     2161 ccaatcaact gaaaattcag gcattcgatg acttcttcgg ttaccgggca ttaatcgacg
     2221 aagttaacgt ctgggttctg ccggaaattg ccgacgagcc agccggtgat ctgatgctaa
     2281 aaggtccaca gggcgaggaa aaagagattg aaagccgcct ggaggaaggt tgctactatt
     2341 tactgttcga cagccgcacc catcgcgggg cgaatcagca agtcagggac tgggtaagct
     2401 atgtgctttc tccaactaat ctggtctatt tcgctgagga acagtaccag caactgtggt
     2461 tcccggctta tggactgctc ccccgttggc accatgcccg caccataaag agcgaaaaac
     2521 cggctggcct ggaaagcctc accctgacct tttatcagga tcacagtgag catcgggtga
     2581 ttgccgggat catgcagcag attctggcaa gtcaccaggt cacgctggaa atcaaagaga
     2641 tcagctacga tcagtggcat gaaggagaga tcgagagcga tatctggctt aatagcgcca
     2701 actttacgct gccgctggat ttttcgctgt tcgcgcactt gtgcgaggtg ccgctgctcc
     2761 aacactgtat tccgatcgac tggcaagccg acgccgcccg ctggcgcaat ggcgaaatga
     2821 acctggcgaa ctggtgccag caactggtcg ccagtaaagc aatggtgccg cttatccacc
     2881 actggctgat cattcaggga caacgcagta tgcgcggcct gcgtatgaat accctcggct
     2941 ggttcgattt taaatcagcg tggtttgcgc caccggatcc atgattgctg gtagataaca
     3001 aaatcactac actaacgccg ttctcaacgg ggtgccacgc aaacgcgtgc gctgagaaaa
     3061 tacccgtcga acctgatccg gataacgccg gcgaagggat ttgaggctcc ttctcaagtc
     3121 ctttgccact cttttttgag gtgcaaagtg ttaaaaaaat gtcttcccct gctgttgctg
     3181 tgcacagcgc ccgttttcgc taaacccgtt ctgactgttt atacctacga ttccttcgcc
     3241 gccgactggg ggcctggtcc aaagatcaaa aaagcttttg aagccgactg taactgcgaa
     3301 ctgaaactgg tggcgctgga agatggcgtt tcgcttctca accgtctacg gatggaaggc
     3361 aaaaatagta aagccgatgt ggtgctgggg ctggataaca atctgttaga cgccgccagt
     3421 aaaaccggac tatttgccaa aagcggtgtg gcagcggatg ccgttaacgt tcccagcggc
     3481 tggaataatg acactttcgt cccgtttgat tacggctatt tcgccttcgt ctatgacaag
     3541 aacaaactga aaaacccgcc gcaaagcctg aaagaattgg ttgagagcga tcagaactgg
     3601 cgggtgattt atcaggatcc gcgtaccagc acgccggggc tgggtctgct gttgtggatg
     3661 caaaaagtct atggcgatga cgccccacaa gcctggcaaa aactggcgaa gaaaaccgtc
     3721 acggtcacca aaggctggag cgaagcctac ggcctgtttt taaaaggtga aagcgatctg
     3781 gtactgagtt acaccacctc tccggcttat cacattctcg aagagaaaaa agataactac
     3841 gccgccgcga actttagcga aggtcactat ctgcaggtgg aagtcgccgc ccgcaccgct
     3901 gccagcaagc agccggagct ggcgcaaaaa ttcctccagt ttatggtttc tccggctttt
     3961 cagaatgcga tcccaaccgg taactggatg tatccggtgg caaatgtcac gctgcctgcc
     4021 gggtttgaac aattgaccaa accggccacc acgctggagt tcacaccagc tgaagtggcg
     4081 gcacaacgtc aggcatggat tagcgaatgg caacgcgccg tcagccgtta attcccggct
     4141 ggttaattcc aggtgtaagc gccgccacgc tggtggtagc ggttgcgctc gcggcgtttc
     4201 tcgccctgtg gtggaacgcg ccgcagggta actgggtggc agtctggcag gacagctacc
     4261 tgtggcatgt ggtgcgcttc tccttctggc aggcgtttct ctcggcgcta ctctctgtcg
     4321 tacccgcgat attcctcgcc cgcgccctct atcgcaggcg ctttccgggt cggctggcgc
     4381 tgttgcgtct gtgcgcaatg accttgatcc tcccggtgct agttgctgtt ttcggcattc
     4441 ttagcgtcta tggtcgtcag ggctggctgg catcgctctg ccaatcgctc ggtctggagt
     4501 ggaccttttc gccctacggc ctgcaaggca ttttgctggc gcacgtattt tttaatctgc
     4561 cgatggcgag ccgcttatta ctccaggcac tggaaaacat tcccggcgaa caacgtcagc
     4621 ttgccgccca gcttgggatg cgcggctggc attttttccg cttcgtcgaa tggccgtggc
     4681 tacggcgaca aatcccgccg gttgccgcgc ttatctttat gctctgtttc gccagcttcg
     4741 ccaccgtgct atcgctgggc ggcggtccgc aggcaaccac tatcgagctg gctatttatc
     4801 aggcgctgag ttacgactac gatcctgccc gcgcggcgat gctggcgctg ctccagatgg
     4861 tgtgttgcct tgggctggtg ctgttgagtc agcgactaag taaggccatt gcgcccggca
     4921 ccacgctgct gcaaggctgg cgcgatccgg acgatcgtct gcatagccgc atttgcgaca
     4981 cggtgttaat agtgctggcg ctgctgctgt tgctgccacc gttgctggcg gtgatcgtcg
     5041 atggggtaaa tcgccagttg ccggaagtgc tggcacaacc ggtgctgtgg caggcgctgt
     5101 ggacctcgtt gcgtattgcg ctggcggcag gtgtattgtg cgtagtgctg accatgatgc
     5161 tgctatggag cagtcgcgaa ctgcgggcgc ggcagaaaat gctggcgggt caggcgctgg
     5221 agatgagcgg catgttgatc ctcgccatgc cggggattgt gctggctacc ggcttctttt
     5281 tactgctcaa caacactatc ggcctgccac aatctgctga cggcattgtg attttcacca
     5341 atgcgttaat ggcgatccct tatgcgctga aagtactgga aaacccgatg cgcgatatca
     5401 ccgcccgcta cagtatgttg tgtcagtcgc tgggcattga aggctggtcg cgcttaaaag
     5461 tggtcgagct gcgcgccctg aaacgtccac tggcgcaggc gctggctttt gcctgcgtgc
     5521 tgtcgattgg tgattttggc gtagtggcgt tgttcggtaa cgatgatttc cgtaccctgc
     5581 cgttttatct ctaccagcaa attggctcct atcgcagcca ggacggcgcg gtcaccgcgt
     5641 taattctgct actactctgt tttctgctgt ttaccgtgat tgaaaaacta ccggggcgaa
     5701 atgttaaaac tgactgatat cacctggctt tatcaccatt tgccgatgcg ttttagctta
     5761 acggtagaac gcggcgagca ggtggcaatc ctcgggccaa gcggcgcggg taaaagtacc
     5821 ctgctgaatt tgattgccgg ttttctgacg ccagccagcg gttcgctgac tatcgatggc
     5881 gttgatcaca ccacgacgcc gccgtcacgc cgtccggtgt cgatgctgtt tcaggagaac
     5941 aacctgttca gccacctgac ggtcgcgcag aacatcgggc tggggctaaa tccggggttg
     6001 aaactgaacg cggcacagca ggagaaaatg cacgcgattg cccgtcagat ggggattgat
     6061 aatttaatgg cgcggttacc gggcgagctt tccggcggtc agcgacagcg cgtggcgtta
     6121 gcgcgttgtc tggtgcgcga acagccgatt ttattgctcg atgaaccgtt ctctgcgctc
     6181 gatccggcgt tacgtcagga gatgctgacg ctggtgagca cgagctgcca gcagcaaaaa
     6241 atgacgctat tgatggtgtc gcacagtgtg gaagatgcgg cgcggatcgc cacacgctcg
     6301 gtggtggtcg ccgacgggcg catcgcctgg cagggtaaga ctgaagaatt gttgagcggt
     6361 aaggcgagtg cttcggcaat attggggatt acgggttagt atgcactgtc agatgcggcg
     6421 tgaacacctt atccgaccta cggacagtgc gttttgtagg cctgataaga cgcggtcagc
     6481 gtcgcatgag gcaacccgca caagacaatc ccctaaaccc caaccacttt acgcaaaata
     6541 tcgatataca ccggcatcaa cgggtggcga attaacacca ccagcgccac cacgccgatg
     6601 gcagaaatca acggcgtcag ccacaacaaa cggccgcgcg acaaataatg acttaaacga
     6661 tcggttgctt taccgctgcg ccataaccgc cagcacagcc agccaccaac ccacaaaaat
     6721 accgccgtcg ccagcagcaa ccatttaaac tcgccgctct gcattccggc tggaatatcg
     6781 atcgccgcgc ccgccagaat cccaggcagg aagtaaaacg gtggccacag caggcagccg
     6841 ataatattcg gcgtaatgaa ttttgccacc ggcagatcca gcattcccgc caccattggc
     6901 accagcggac gcgtcgggcc aacaaagcga ccgaccagaa tggtgaacat gctgtgttga
     6961 tgcaacgcat gttcagtttt atcgagtaat gctttgtttt tcttcagaaa tgaccagcga
     7021 tgcaacggct ttttaaaacg ccagcccagc cagaaggaga tccagtcgcc cagcagacaa
     7081 ccgacaatcc ctgccagcca agcgtgccag aaacttaact cgccgctgcc aatcagcgct
     7141 cccagccccg ccatcagcac cgtaccgggt agaatcaaac cgaccagcgc cagcgactcc
     7201 agaaaggcca ccaacaccac cgccatcaat gaatacacgg tagattgggt aataaagtgt
     7261 tccagcaatg cttgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct
     7321 atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc
     7381 tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca
     7441 ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt
     7501 ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc
     7561 gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg
     7621 cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc
     7681 gctgatgtac tgacaagcct cgcgtacacg attatccatc ggtggatgga gcgactcgtt
     7741 aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga
     7801 atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatacgg
     7861 ctggtgcgct tcatccgggc gaaagaaacc cgtattggca aatattgacg gccagttaag
     7921 ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc
     7981 ctccggatga caaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg
     8041 tcggcagaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt
     8101 gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc
     8161 ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg
     8221 atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag aaaccaattg
     8281 tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct cgctaaccca
     8341 accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag ccatgacaaa
     8401 aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt atttgcacgg
     8461 cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc ctacctgacg
     8521 ctttttatcg caactctcta ctgtttctcc atacccgttt ttttggatgg agtgaaacga
     8581 tggcgattgc aattggcctc gattttggca gtgattctgt gcgagctttg gcggtggact
     8641 gcgccagcgg tgaagagatc gccaccagcg tagagtggta tccccgttgg caaaaagggc
     8701 aattttgtga tgccccgaat aaccagttcc gtcatcatcc gcgtgactac attgagtcaa
     8761 tggaagcggc actgaaaacc gtgcttgcag agcttagcgt cgaacagcgc gcagctgtgg
     8821 tcgggattgg cgttgacagt accggctcga cgcccgcacc gattgatgcc gacggtaacg
     8881 tgctggcgct gcgcccggag tttgccgaaa acccgaacgc gatgttcgta ttgtggaaag
     8941 accacactgc ggttgaagaa gcggaagaga ttacccgttt gtgccacgcg ccgggcaacg
     9001 ttgactactc ccgctatatt ggcggtattt attccagcga atggttctgg gcaaaaatcc
     9061 tgcatgtgac tcgccaggac agcgccgtgg cgcaatctgc cgcatcgtgg attgagctgt
     9121 gcgactgggt gccagctctg ctttccggta ccacccgccc gcaggatatt cgtcgcggac
     9181 gttgcagcgc cgggcataaa tctctgtggc acgaaagctg gggcggcttg ccgccagcca
     9241 gtttctttga tgagctggac ccgatcctca atcgccattt gccttccccg ctgttcactg
     9301 acacctggac tgccgatatt ccggtgggca ccttatgccc ggaatgggcg cagttggcgc
     9361 aggcgcacag cctaacgcac tggtaaaagt tatcggtact tccacctgcg acattctgat
     9421 tgccgacaaa cagagcgttg gcgagcgggc agttaaaggt atttgcggtc aggttgatgg
     9481 cagcgtggtg cctggattta tcggtctgga agcaggccaa tcggcgtttg gtgatatcta
     9541 cgcctggttt ggtcgcgtac tcggctggcc gctggaacag cttgccgccc agcatgcgga
     9601 actgaaagcg caaatcaacg ccagccagaa acaactgctt ccggcgctga ccgaagcatg
     9661 ggccaaaaat ccgtctctgg atcacctgcc ggtggtgctc gactggttta acggtcgtcg
     9721 cacgccaaac gctaaccaac gcctgaaagg ggtgattacc gatcttaacc tcgctaccga
     9781 cactccgctg ctgttcggcg gtttgattgc tgccaccgcc tttggcgcac gcgcaatcat
     9841 ggagtgcttt accgatcagg ggatcgccgt caataacgtg atggcgctgg gcggcatcgc
     9901 gcggaaaaac caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc cgctgcaaat
     9961 tgttgcctct gaccagtgct gtgcgctcgg tgcggcgatt tttgctgccg tcgccgcgaa
    10021 agtgcacgca gacatcccat cagcccagca aaaaatggcc agtgcggtag agaaaaccct
    10081 gcaaccgcgc agcgaacagg cacaacgctt tgaacagctt tatcgccgct atcagcaatg
    10141 ggcgatgagc gccgaacaac actatcttcc aacttccgcc ccggcacagg ctgcccaggc
    10201 cgttgcgact ctataaggac acgataatga cgatttttga taattatgaa gtgtggtttg
    10261 tcattggcag ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc caacatgccg
    10321 agcacgttgt taatgcgctg aatacggaag cgaaactgcc ctgcaaactg gtgttgaaac
    10381 cgctgggcac cacgccggat gaaatcaccg ctatttgccg cgacgcgaat tacgacgatc
    10441 gttgcgctgg tctggtggtg tggctgcaca ccttctcccc ggccaaaatg tggatcaacg
    10501 gcctgaccat gctcaacaaa ccgttgctgc aattccacac ccagttcaac gcggcgctgc
    10561 cgtgggacag tatcgatatg gactttatga acctgaacca gactgcacat ggcggtcgcg
    10621 agttcggctt cattggcgcg cgtatgcgtc agcaacatgc cgtcgttacc ggtcactggc
    10681 aggataaaca agcccatgag cgtatcggct cctggatgcg tcaggcggtc tctaaacagg
    10741 atacccgtca tctgaaagtc tgccgatttg gcgataacat gcgtgaagtg gcggtcaccg
    10801 atggcgataa agttgccgca cagatcaagt tcggtttctc cgtcaatacc tgggcggttg
    10861 gcgatctggt gcaggtggtg aactccatca gcgacggcga tgttaacgcg ctggtcgatg
    10921 agtacgaaag ctgctacacc atgacgcctg ccacacaaat ccacggcgaa aaacgacaga
    10981 acgtgctgga agcggcgcgt attgagctgg ggatgaagcg tttcctggaa caaggtggct
    11041 tccacgcgtt caccaccacc tttgaagatt tgcacggtct gaaacagctt ccaggtctgc
    11101 ccgtacagcg tctgatgcag cagggttacg gctttgcggg cgaaggcgac tggaaaaccg
    11161 ccgccctgct tcgcatcatg aaggtgatgt caaccggtct gcagggcggc acctccttta
    11221 tggaggacta cacctatcac ttcgagaaag gtaatgacct ggtgctcggc tcccatatgc
    11281 tggaagtctg cccgtcgatt gccgtagaag agaaaccgat cctcgacgtt cagcatctcg
    11341 gtattggtgg taaggacgat cctgcccgac tgatcttcaa tacccaaacc ggtccagcga
    11401 ttgtcgccag cctgattgat ctcggcgatc gttaccgtct gctggttaac tgtatcgaca
    11461 cggtgaaaac accgcactcc ctgccgaaac tgccggtggc taatgcactg tggaaagcgc
    11521 aaccggatct gccaactgct tccgaagcgt ggatcctcgc tggtggcgcg caccataccg
    11581 tcttcagcca tgcgctgaac ctcaacgata tgcgccagtt cgccgagatg cacgacattg
    11641 aaatcacggt gattgataac gacacccgcc tgccagcgtt taaagacgca ctgcgctgga
    11701 acgaagtgta ttacggattt cgtcgctaag tagccacatc aggtatgtaa cgcctgatgc
    11761 gacgctgact cgtcttatct ggcctacacg ctgcgatttt gtaggccgga taagcaaagc
    11821 gcatccggca ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct acgcgctgcg
    11881 attttgtaga ccggataagc gaagcgcatc cggcacgaag gagtcaacat gttagaagat
    11941 ctcaaacgcc aggtattaga agccaacctg gcgctgccaa aacacaacct ggtcacgctc
    12001 acatggggca acgtcagcgc cgttgatcgc gagcgcggcg tctttgtgat caaaccttcc
    12061 ggcgtcgatt acagcgtcat gaccgctgac gatatggtcg tggttagcat cgaaaccggt
    12121 gaagtggttg aaggtacgaa aaagccctcc tccgacacgc caactcaccg gctgctctat
    12181 caggcattcc cctccattgg cggcattgtg catacgcact cgcgccacgc caccatctgg
    12241 gcgcaggcgg gtcagtcgat tccagcaacc ggcaccaccc acgccgacta tttctacggc
    12301 accattccct gcacccgcaa aatgaccgac gcagaaatca acggcgaata tgagtgggaa
    12361 accggtaacg tcatcgtaga aacctttgaa aaacagggta tcgatgcagc gcaaatgccc
    12421 ggcgttctgg tccattccca cggcccgttt gcatggggca aaaatgccga agatgcggtg
    12481 cataacgcca tcgtgctgga agaggtcgct tatatgggga tattctgccg tcagttagcg
    12541 ccgcagttac cggatatgca gcaaacgctg ctggataaac actatctgcg taagcatggc
    12601 gcgaaggcat attacgggca gtaatgactg tataaaacca cagccaatca aacgaaacca
    12661 ggctatactc aagcctggtt ttttgatgga ttttcagcgt ggcgcaggca ggttttatct
    12721 taacccgaca ctggcgggac accccgcaag ggacagaagt ctccttctgg ctggcgacgg
    12781 acaacgggcc gttgcaggtt acgcttgcac cgcaagagtc cgtggcgttt attcacgccg
    12841 atcaggttcc ccgcgctcag catattttgc agggtgaaca aggctttcgt ctgacaccgc
    12901 tggcgttaaa ggattttcac cgccagccgg tgtatggcct ttactgtcgc gcccatcgcc
    12961 aattgatgaa ttacgaaaag cgcctgcgtg aaggtggcgt taccgtctac gaggccgatg
    13021 tgcgcccgcc agaacgctat ctgatggagc ggtttatcac ctcaccggtg tgggtcgagg
    13081 gtgatatgca caatggcact atcgttaatg cccgtctgaa accgcatccc gactatcgtc
    13141 cgccgctcaa gtgggtttct atagatattg aaaccacccg ccacggtgag ctgtactgca
    13201 tcggcctgga aggctgcggg cagcgcatcg tttatatgct ggggccggag aatggcgacg
    13261 cctcctcgct tgatttcgaa ctggaatacg tcgccagccg cccgctgctg ctggaaaaac
    13321 tcaacgcctg gtttgcaaac cacaatcccg atgtgatcat cggctggaac gtggtgcagt
    13381 tcgatctgcg aatgctgcaa aaacatgccg agcgttaccg tcttccgctg cgtcttgggc
    13441 gcgataatag cgagctggag tggcgcgagc acggctttaa aaacggcgtc ttttttgccc
    13501 aggctaaagg tcggctaatt atcgacggta tcgaggcgct gaaatccgcg ttctggaatt
    13561 tctcttcatt ctcgctggaa actgtcgctc aggagctatt aggcgaagga aaatctatcg
    13621 ataacccgtg ggttcgaatg gacgaaattg accgccgttt cgccgaagat aaacctgcgc
    13681 tggcaactta taacctgaaa gattgcgagc tggtgacgca gatcttccac aaaactgaaa
    13741 tcatgccatt tttactcgaa cgagcaacgg tgaacggcct gccggtggat cggcacggcg
    13801 gttcggtggc agcgtttggt catctctatt ttccgcgaat gcatcgcgct ggttatgtcg
    13861 cgcctaatct cggcgaagtg ccgccgcacg ccagccctgg cggctacgtg atggattcac
    13921 ggccagggct ttatgattca gtgctggtgc tggactataa aagcctgtac ccgtcgatca
    13981 tccgcacctt tctgattgat cccgtcgggc tggttgaagg catggcgcaa cctgatccag
    14041 agcacagtac cgaaggtttt ctcgatgcct ggttctcacg ggagaaacat tgcctgccgg
    14101 agattgtgac taacatctgg cacgggcgcg atgaagccaa acgtcagggt aataagccgc
    14161 tttcgcaggc gctgaagatc attatgaatg ccttttatgg cgtgctcggc accaccgcct
    14221 gccgcttctt cgatccgcgc ctggcatcgt cgatcaccat gcgtggtcat cagatcatgc
    14281 ggcagaccaa agcattgatt gaagcgcagg gctacgacgt tatctacgga gataccgact
    14341 cgacttttgt ctggctaaaa ggtgcacatt cggaagaaga ggcggcgaaa atcggtcgtg
    14401 cactggtgca gcacgttaac gcctggtggg cggaaacgct gcaaaaacaa cggctgacca
    14461 gcgcattaga actggagtat gaaactcatt tctgccgttt tcttatgcca accattcgcg
    14521 gagctgatac cggcagtaaa aagcgctacg ccgggctgat tcaggagggc gacaagcagc
    14581 ggatggtgtt taaagggctg gaaaccgtgc gcaccgactg gacgccgctg gcccagcagt
    14641 ttcagcagga gctgtacctg cgtatcttcc gcaacgagcc atatcaggag tacgtccgcc
    14701 aaaccatcga caaactgatg gcgggtgaac tggatgcgcg gctggtttac cgtaaacgcc
    14761 ttcgccgtcc gctgagcgaa tatcagcgta atgttccgcc tcatgtacgc gccgctcgcc
    14821 ttgccgatga agaaaatcaa aagcgcggtc gccccttgca atatcagaat cgcggcacca
    14881 ttaagtacgt atggaccacc aacggcccgg agccgctgga ctaccaacgt tcaccactgg
    14941 attacgaaca ctatctgacc cgccagctac aacccgtggc ggagggaata ctccctttta
    15001 ttgaggataa ttttgctaca cttatgaccg ggcaacttgg gctattttga gcaaaaaaag
    15061 agttcgccag ataccatttt gatgcgtgac gaatgctttg ccatccagta ccatagcgcc
    15121 ctttccattc ctggacctga ataacaccac tacctcataa gcacggtagc gggtggttat
    15181 tgcctgcaat taaagatata gagccgaaca catatgcctt ttacacttgg tcaacgctgg
    15241 atcagcgata cagaaagcga attgggactt ggaaccgttg tcgcggtgga tgcgcgaact
    15301 gtcactttac ttttcccatc tactggtgaa aaccgtctgt acgcacgcag tgattccccc
    15361 gtgacccgcg tgatgttcaa ccctggtgat accattacca gccatgacgg ctggcagatg
    15421 caagtcgaag aagtaaaaga agaaaatggc ttgctgacct atatcggtac tcgcctggat
    15481 actgaagagt ccggcgtagc cctgcgtgaa gttttccttg atagcaaact ggtgttcagc
    15541 aaaccgcagg accgtctgtt tgccgggcag attgaccgta tggaccgctt tgcgctgcgt
    15601 tatcgcgcgc gtaagtattc cagcgaacag ttccgtatgc cgtacagcgg cctgcgcggt
    15661 cagcgtacca gcctgatccc gcatcagctc aacatcgctc atgatgttgg tcgccgccac
    15721 gcgccgcgcg tcctgctggc tgacgaagtg ggtttaggga aaaccattga agccgggatg
    15781 atcctgcatc agcaactgct ctctggcgct gctgaacgtg tgctgattat cgtcccggaa
    15841 accttacagc atcagtggct ggtagaaatg ctgcgccgtt tcaacctgcg ctttgctctg
    15901 tttgatgatg agcgttatgc cgaagctcag cacgatgctt acaacccgtt tgacaccgaa
    15961 cagctggtga tttgctcgct ggattttgcc cgtcgtagca aacagcgcct ggaacatctc
    16021 tgtgaagccg aatgggacct gctggtggtc gatgaagcgc atcacctggt gtggagcgaa
    16081 gatgcgccaa gccgtgaata tcaggccatt gaacaactgg cagagcacgt gccgggcgtt
    16141 ctgctgctga ccgcgacccc ggaacagctg gggatggaaa gccacttcgc ccgtctgcgt
    16201 ctgctggacc cgaaccgttt ccacgatttt gcgcagttcg ttgaagagca gaaaaattat
    16261 cgtccggttg cggacgccgt tgccattctg ctggcaggta acaaactgag caatgacgaa
    16321 ctgaacatgc tcggcgagat gatcggcgag caggatatcg agccgctgtt gcaggcagca
    16381 aacagcgaca gcgaagatgc ccagagcgcc cgtcaggagc tggtttcgat gctgatggat
    16441 cgccacggca ccagccgcgt gctgttccgt aacacgcgta acggtgtgaa aggattcccg
    16501 aaacgcgagc tgcacaccat taagctgccg ctaccgacgc agtatcagac ggctattaaa
    16561 gtctccggca ttatgggcgc acgtaaaagt gcggaagatc gtgctcgcga tatgctctac
    16621 ccggagcgta tttatcagga atttgaaggt gataacgcca cctggtggaa cttcgatccg
    16681 cgcgttgagt ggctgatggg ctacctgacc agccatcgct ctcagaaagt gctggtgatc
    16741 tgcgccaaag ctgccactgc gctgcaactg gagcaggtac tgcgcgaacg tgaaggtatt
    16801 cgcgctgcgg tgttccacga aggtatgtcg attatcgaac gtgaccgcgc tgccgcctgg
    16861 tttgccgaag aagacaccgg cgcacaggta ctgctgtgct cagaaatcgg ttctgaagga
    16921 cgtaactttc agttcgccag ccacatggtg atgtttgacc tgccattcaa cccggatcta
    16981 ctggagcagc gtattggtcg tctggatcgt atcggccagg cgcacgatat tcagatccat
    17041 gtgccttatc tggagaaaac cgctcagtcg gtgctggtgc gctggtatca cgaaggtctg
    17101 gatgcatttg agcacacctg cccgaccgga cgcactattt acgatagcgt atacaacgat
    17161 ctgattaact atctggcttc accggatcaa accgaaggct ttgacgatct gatcaaaagc
    17221 tgccgcgagc aacatgaagc gctgaaagca cagctggaac agggtcgtga ccgcctgctg
    17281 gaaatccact ccaacggtgg cgaaaaagcc caggcactgg cagaaagcat tgaagagcag
    17341 gatgacgata ccaacctgat cgccttcgcc atgaacctgt tcgatattat cggtatcaat
    17401 caggacgatc gcggcgacaa catgatcgtg ctgacgccgt ccgatcatat gctggtgccg
    17461 gacttccctg gcctgtcgga agatggcatc accatcacct ttgatcgtga agtggcgctg
    17521 gcgcgtgaag atgcacagtt tattacctgg gagcatccgc tgatccgcaa cggtctggat
    17581 ctgatccttt ctggcgatac cggtagcagc acgatttcac tgttaaaaaa caaagcgttg
    17641 ccggtaggta cgctgttggt ggaactgatt tatgtggttg aagcccaggc tccgaagcag
    17701 ttgcagctca accgcttcct gccaccgacg ccggtacgta tgctgctgga taaaaacggc
    17761 aacaacctgg cggcgcaggt agagtttgaa acctttaacc gccagcttaa cgcggttaac
    17821 cgtcacaccg gcagcaaact ggttaacacc gtgcagcagg atgttcacgc tatccttcaa
    17881 ctgggtgaag cgcagatcga gaaatctgcc cgtgcattga ttgatgcagc gcgtaacgaa
    17941 gccgacgaaa aactgtctgc cgagctgtct cgtctggaag ctctgcgtgc agtgaacccg
    18001 aacattcgtg acgacgaact gaccgccatt gagagcaacc gtcagcaggt aatggaaagc
    18061 ctggatcagg caggttggcg tctggatgcc ctgcgtttga tcgttgtaac gcatcagtaa
    18121 cggagccgaa aatggggatg gaaaactaca atccaccgca ggaaccctgg ttggttatcc
    18181 tgtatcagga tgaccatatt atggtggtca acaagccgag cggtttgttg tcagtgccgg
    18241 gtcgtctgga agagcacaaa gacagcgtga tgacgcgcat tcagcgtgat tatccgcagg
    18301 cagaatcggt gcatcgtctg gatatggcta ccagcggcgt gattgtagtg gcgctgacca
    18361 aagccgcgga gcgggagtta aaacgccagt tccgcgagcg cgagccgaaa aagcagtatg
    18421 tggcccgcgt ctgggggcat ccatcccccg cagaaggtct ggtggatctg ccgctgattt
    18481 gcgactggcc aaaccgcccg aaacagaaag tctgttacga aacgggtaaa cctgcgcaga
    18541 cggaatatga agtggtggag tatgcggcgg ataacacggc aagagcggtg ttaaaaccga
    18601 ttaccgggcg ttcgcatcaa ttgcgtgtgc atatgctggc gctggggcat ccgattctcg
    18661 gcgatcgttt ttatgcatca ccagaagcga gagcgatggc accgcgtttg ttgctacatg
    18721 cagagatgtt gacgattacc catccggcgt atggcaatag tatgacgttt aaagcgccag
    18781 cggattttta atcgtcacgc tggcgcagtt cggtaggcgg ataaggcgtt cacgccgcat
    18841 ccggcaattg tgcgccgatt gcctgatgcg acgcgggccg cgtcttatca ggcctacagt
    18901 ttaaaactta tttaaaccct ttctgctgct ttatcagctc atatgcctgc tgaatttcct
    18961 gcgctttctg cttcgccatc tccatcatct caggcggcaa acctttcgcc accagcttat
    19021 cgggatggtg ttcactcatc agcttacggt aggcacgttt gatggtggtc gcatcatccg
    19081 tcggcttcac gcccagcaca ttacaggcat cttccagcgt cgggccacgc tgcgcttgct
    19141 gccagttacc accgccagat tgctgctgat aaccgccgcc aaactgtgca ccgccctgca
    19201 tcatgcgcaa aaactggtca aactgggcgc gggagatccc taattcttca gcaatgacat
    19261 acagcaccgc ccgttcattc gggtgcagtg aaccatcagc aaacgccgcc tgaatctgga
    19321 tctccagaaa catacgaatt aagtcaaaac gaccaaagca gacactgcga aactggcgca
    19381 tcttttcgcg cagcgggtaa ttatctgatt ttcccacccg gaacgcgttt tgcgccgcag
    19441 tacgggaagc gccatgaagg ttcattcggt ccatcaactg gctggcgata tgaatatcag
    19501 cctccgtgac gcgacctttg gatttggtta aatgccccat cacttcaaaa gtggtggcaa
    19561 aaaacagcgc ctgacgctca cgctggttgg cgaaccacgc cattttacgg ctacgggctt
    19621 tatcaaacat atggccaatt aacaggccta acactacgcc ccaaaagccg ccgcccatca
    19681 gtaaggccac ggccacgcca atgatttttc cccaatactg catatattcc ccaaatcgac
    19741 acacggatat cagggctatc tcccacaata taaaggtgct tttaccgttt tccggctgcg
    19801 gtcaattgtg ggacatcgcc tataatttgc attatcatac ctgtcattca atgccgtgcc
    19861 taacaccact gacgctattc ggacaggatt aacactagcg tagagatgac aagtacgtta
    19921 gtctctgacc gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa tcaacgtatg
    19981 aaaaaacgta tccccactct cctggccacc atgattgcca ccgcccttta tagtcaacag
    20041 ggactggcag ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta tgaccgtcct
    20101 ctggtacagg gcgataccaa tgacttaccc gtgactatca atgctgacca cgcgaaaggg
    20161 gactacccgg atgacgccgt gtttactggc agcgtggata tcatgcaggg taacagccgt
    20221 ctgcaggccg acgaagtgca gctccatcaa aaagaggcac caggacaacc ggagccggta
    20281 cgtaccgttg atgcgctcgg taatgtccat tacgacgata accaggtgat cctcaaaggg
    20341 ccgaaaggct gggcgaatct gaacaccaaa gataccaacg tctgggaagg tgattaccag
    20401 atggtgggtc gccagggtcg cggtaaagcg gacctgatga aacaacgtgg cgaaaaccgc
    20461 tataccattc tggataacgg tagctttacc tcctgtctgc cgggttctga cacctggagc
    20521 gtggtaggta gcgaaattat tcatgaccgc gaagaacaag ttgcggagat ctggaacgcc
    20581 cgctttaagg tgggtccggt accgatcttt tatagcccct atttgcagtt gccggtgggt
    20641 gacaaacgtc gctctggttt cttgatcccg aacgccaagt acaccaccac caactacttt
    20701 gagttctacc tgccatatta ctggaacatc gcgccaaata tggatgccac catcacgccg
    20761 cattatatgc atcgtcgtgg caacatcatg tgggagaacg aattccgcta cctctcccag
    20821 gcgggcgctg gcttgatgga actggactat ctgccttcag ataaagtcta tgaagatgaa
    20881 cacccgaacg atgacagttc acgtcgttgg ttattctact ggaaccactc cggggtcatg
    20941 gatcaggtgt ggcgtttcaa cgtcgactac accaaggtca gcgatcctag ctacttcaat
    21001 gatttcgata acaagtacgg ttccagtact gacggctacg caacgcaaaa attcagcgtt
    21061 ggctatgcgg tgcaaaactt caatgccacc gtttcaacca agcagttcca ggttttcagc
    21121 gaacagaaca ccagtagcta ctcggcagag ccgcagttag acgttaatta ctaccagaat
    21181 gatgttggtc cgtttgatac gcgtatttac ggccaggcag tgcactttgt taacaccaga
    21241 gacgacatgc ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt gccgctctct
    21301 aataactggg gcagcatcaa taccgaagcg aagtttctgg caacccatta tcagcaaacc
    21361 aatcttgact ggtataactc cagaaacacg accaagctgg acgaatccgt taaccgcgta
    21421 atgccgcaat tcaaagttga cggcaaaatg gtctttgaac gcgatatgga aatgctggct
    21481 ccgggttata cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc gtatcgcgat
    21541 cagagcgaca tctataacta cgactcgtct ctgctgcaat ctgactactc tggcctgttc
    21601 cgggaccgga cttacggcgg tcttgaccgt attgcctccg ctaaccaggt gacgaccggt
    21661 gtcacatctc gcatatatga tgatgctgcc gttgaacgtt ttaatatttc cgttggtcaa
    21721 atctactatt tcacggagtc tcgcactggc gatgacaaca taacatggga gaatgacgac
    21781 aaaacgggct cactggtgtg ggcaggcgac acctactggc gtatctccga gcgttgggga
    21841 ttgcgtggcg ggattcagta cgatacacgt ctggataacg tagcgaccag taactccagc
    21901 attgaatacc gtcgggatga agaccgtctg gtacagctga attaccatta cgccagcccg
    21961 gaatatattc aggctacgct gcctaagtac tattccactg ctgagcaata taagaatggt
    22021 atttcgcagg taggtgctgt cgccagcagg ccaattgccg atcgttggtc cattgttggg
    22081 gcctactact acgacaccaa tgcgaacaag caagccgact ctatgttagg tgtgcaatac
    22141 agctcctgct gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg ttgggataac
    22201 gataaacaac atgcggtata tgacaacgca atcggcttta acatcgaact tcgcggcctg
    22261 agctccaact acggtctggg tacgcaagag atgctgcgtt cgaacattct gccgtatcaa
    22321 aacactttgt gatttgttga tttaccacgt aatccgcagt gcggttaatt gaaatggaaa
    22381 aagtatgaag aactggaaaa cgctgcttct cggtatcgcc atgatcgcga ataccagttt
    22441 cgctgccccc caggtagtcg ataaagtcgc agccgtcgtc aataacggcg tcgtgctgga
    22501 aagcgacgtt gatggattaa tgcagtcggt aaaactgaac gctgcgcagg caaggcagca
    22561 gcttcctgat gacgcgacgc tgcgccacca aatcatggaa cgtttgatca tggatcaaat
    22621 catcctgcag atggggcaga aaatgggagt gaaaatctcc gatgagcagc tggatcaggc
    22681 gattgctaac atcgcgaaac agaacaacat gacgctggat cagatgcgca gccgtctggc
    22741 ttacgatggt ctgaactaca acacttatcg taaccagatc cgcaaagaga tgattatctc
    22801 tgaagtgcgt aacaacgagg tgcgtcgtcg catcaccatc ctgccgcagg aagtcgaatc
    22861 cctggcgcag caggtgggta accaaaacga cgccagcact gagctgaacc tgagccacat
    22921 cctgatcccg ctgccggaaa acccgacctc tgatcaggtg aacgaagcgg aaagccaggc
    22981 gcgcgccatt gtcgatcagg cgcgtaacgg cgctgatttc ggtaagctgg cgattgctca
    23041 ttctgccgac cagcaggcgc tgaacggcgg ccagatgggg tggggccgta ttcaggagtt
    23101 gccggggatc ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg ttggcccgat
    23161 tcgttccggc gttggcttcc atattctgaa agttaacgac ctgcgcggcg aaagcaaaaa
    23221 tatctcggtg accgaagttc atgctcgcca tattctgctg aaaccgtcgc cgatcatgac
    23281 tgacgaacag gcccgcgtga aactggagca aattgctgct gatatcaaga gtggtaaaac
    23341 gacttttgct gccgcagcga aagagttctc gcaggatcca ggttctgcta accagggcgg
    23401 tgatctcggc tgggctacac cagatatttt cgatccagcc ttccgtgacg ccctgacccg
    23461 cctgaacaaa ggtcaaatga gtgcaccggt tcactcttca ttcggctggc atttaatcga
    23521 actgctggat acccgtaatg tcgataaaac cgacgctgcg cagaaagatc gtgcataccg
    23581 catgctgatg aaccgtaagt tctcggaaga agcagcaagc tggatgcagg aacaacgtgc
    23641 caacgcctac gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt tgtgatcact
    23701 cccggcgagc ccgccgggat tggcccggac ttagttgtcc agcttgcaca gcgtgagtgg
    23761 ccggtcgaac tggttgtttg tgccgatgcc actctcctta ccgaccgggc agcgatgctc
    23821 ggtttgccgc tcaccctccg cccttattcc cccaactccc ctgcacaacc gcaaactacg
    23881 ggcacattaa cgctacttcc tgtcgcgcta cgtgaatctg tcactgcggg gcagttagcg
    23941 attgaaaatg ggcattacgt ggtggagacg ctggcgcgag cgtgtgatgg ctgtctgaac
    24001 ggtgaatttg ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa cgacgcaagc
    24061 attccgttta ccggtcatac cgagtttttc gaagagcgtt cgcaggcgaa aaaagtggtg
    24121 atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt accgctgcgc
    24181 gatatcgcag atgctatcac ccctgcactt ttgcacgaag tgattgctat tttgcatcac
    24241 gatttgcgta caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg gctgaatccg
    24301 cacgcgggcg aaggcggtca tatgggtacg gaagagatag acaccattat tccggtgctc
    24361 gacgagctgc gggcgcaggg aatgaaactc aacgggccgc tgcctgccga taccctgttt
    24421 cagccgaaat atctcgataa cgccgacgcc gtgctggcga tgtaccacga tcagggtctt
    24481 cccgtgctaa aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg cctgcccttt
    24541 attcgcacat cagtggacca cggcaccgcg cttgaactgg cgggacgtgg cgaagccgat
    24601 gtcggcagtt ttattacggc gcttaatctc gccatcaaaa tgattgttaa cacccaatga
    24661 ataatcgagt ccaccagggc cacttagccc gtaaacgctt cgggcaaaac tttctcaacg
    24721 atcagttcgt gatcgacagc attgtttctg ccattaaccc gcaaaagggc caggcaatgg
    24781 tcgaaatcgg ccccggtctt gcggcattga ccgagccggt cggcgaacgt ctggaccagc
    24841 tgacggtcat cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat ccattcttag
    24901 gcccgaaact gacgatttat cagcaggatg cgatgacctt taactttggc gaactggccg
    24961 agaaaatggg tcagccgctg cgtgttttcg gcaacctgcc ttataacatc tccacgccgt
    25021 tgatgttcca tctgtttagc tatactgatg ccattgccga catgcacttt atgttgcaaa
    25081 aagaggtggt gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt cgattaagcg
    25141 tcatggcgca atactattgc aacgtgatcc cggtactgga agttccgccg tcagccttta
    25201 caccaccacc caaagtggat tccgccgtcg tgcgcctggt ccctcatgca acgatgcctc
    25261 acccggttaa agatgttcgc gtgttgagcc gcatcaccac cgaagccttt aaccagcgtc
    25321 gtaaaaccat tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta accggaatgg
    25381 ggatcgaccc ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc cagatggcga
    25441 actatctggc ggagaacgcg cctttgcagg agagttaacg atgatcaatt cgccccgagt
    25501 gtgtattcag gttcaaagcg tctacattga ggcacaatct tcacctgata atgaacgtta
    25561 cgtttttgct tataccgtaa ccatacgcaa tctggggcga gcgccagtgc agttgttggg
    25621 gcgttactgg ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg gcgaaggagt
    25681 ggttggcgtc cagccactta tcgcgcctgg cgaagagtac cagtacacca gcggtgcaat
    25741 cattgaaacc ccgctgggca ccatgcaggg tcactacgaa atgatcgatg aaaatggcgt
    25801 ccctttcagc atcgacattc ccgtattccg actcgccgtt cccacactca ttcattaaaa
    25861 gaatatggcg acatacctta ttggcgacgt tcatggttgt tacgatgaac tgatcgcatt
    25921 gctgcataaa gtagaattta cccccgggaa agataccctc tggctgacgg gcgatctggt
    25981 cgcgcgcggc ccgggttcgc tggatgttct gcgctatgtg aaatccttag gcgacagcgt
    26041 acgtctggtg ctgggcaatc acgatctgca tctgctggcg gtatttgccg ggatcagccg
    26101 caataaaccg aaagatcgcc tgacaccgct gctggaagcg ccggatgccg acgagctgct
    26161 taactggctg cgtcgccagc ctctgctgca aatcgacgaa gagaaaaagc tggtgatggc
    26221 ccacgcaggg atcacgccgc agtgggatct gcagaccgcc aaagagtgcg cgcgcgatgt
    26281 agaagcggtg ctgtcgagtg actcctatcc cttctttctt gacgctatgt acggcgatat
    26341 gccaaataac tggtcaccgg aattgcgggg gctgggaaga ctgcgtttta tcaccaacgc
    26401 ctttacccgt atgcgttttt gcttcccgaa cggtcaactg gatatgtaca gcaaagaatc
    26461 gccggaagaa gcccctgccc cactgaaacc gtggtttgcg attcctggcc ctgtcgctga
    26521 agaatacagc atcgcctttg gtcactgggc atcgctggaa ggcaaaggta cgccggaagg
    26581 tatttacgcg ctggataccg gctgctgctg gggtgggtca ttaacctgcc tgcgctggga
    26641 agataaacag tattttgtcc agccgtcgaa ccggcataag gatttgggcg aagcggcggc
    26701 gtcttaaaca cagcctgata caggaaggcc ggataagacg cgaccggcgt cgcatccggc
    26761 actagccgta aattctatac aaacttaccg ccgctccaga atctcaaagc aatagctgtg
    26821 agagttctgc gcatcggcgt cgtggaattc gctgaatacc gattcccagt catccggctc
    26881 gtaatccggg aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca gatacagttt
    26941 ttgcgctttt ggcaggaact gctcataaac gcgaccgccg ccaatcacca tgatttctgg
    27001 tacgtcacca cacgccgcaa tggcttcatc caccgatttc acccacgtta cgcgatcgtc
    27061 cgtacccggt tgactgctga ggataatatt tttgcgtcct ggcaacggac gaccgattga
    27121 ttcccaagta tggcggccca taatcacggg tttatttaag gtgttgcgtt taaaccaggc
    27181 gagatcggca ggcaggttcc acggcatggc gttttccatg ccgataacgc gatctaccgc
    27241 taacgccgca atcagactga tcattgagat ttcccgataa aaaaattgtc gccactatac
    27301 gtaaagcgta aaccgtcgtc gactggtgcg aggatgatgt tgaggaaaat tttatattct
    27361 gctggcgagt ccacgctctc ccccaggact cgccgcatta caatgaaaca aaaacaaaca
    27421 gttagctgta aagtgtgatt tacgtcactc tttattaaga tgagggtttc gtttccggtt
    27481 catccgccat gttgccggta tgtttacctt cttctgttcc ctgccagcca tgtcgttgaa
    27541 ttaatgacag atgttcgcgg tcctcggtga tgatctcact taacatcgcg ctggtgcgtt
    27601 tatagaccgc cgcgcgggct ttggtgtcat tctcaaccat tgccatctct tccaccatct
    27661 gaatattaaa gcggcggaac acatcggcac gttctcgcgc ttcatacggc cccagaccta
    27721 aactttccag cgccagacgc ccggttttca gcgcaccttc aaaggtttca cgctccggct
    27781 tttcaacgcc tgcctgacgc aaacgaatgt agtgatcgac atcgcgggcg cgggcaataa
    27841 tctgcaaatg cgggaaatgt tctttcacca tctccgtcag ttgcaggttg gtttgcggat
    27901 cgtcaatagc gttaatcagc acttccgctt tcgccgctcc ggcagattcc agtaaatcca
    27961 tccgcgtggc atcgccataa aacactttca taccaaattt acgcagcgtt tcgatatggt
    28021 ccggatcgtg atcgagcacc accattttca ccccgctgga gagcagtaaa cgtccggtaa
    28081 tctgtccaaa acgaccgaat ccggcgataa tcacgcgcgg ctgttcttca tcgatctcat
    28141 cggcttcacg cgcctcctcc gtagaagatt gctcaaggcg attgaggatc accagcagaa
    28201 tcggcgttgc tgccatcgac agcgccaccg ccagggtcag cgatttcgcc cactccggct
    28261 ccagcacatt cgccatctgc gccgcaccaa ataccacaaa ggcaaactca ctgccctgcc
    28321 ctaacaacac cgcaaaccag cgacgctgtt tatttggcac ctgcaacggt cgggcaatca
    28381 gccacagcat ggcgattttg atgatgagga aaccgagcag caaaatgaca atgcgcaatg
    28441 ggttttcaat cagcgtgcca aagtctatcg acatgccaac accgatgaaa aacagcccca
    28501 acagcaaacc tttaaacggt tcgatatcgc tttccaacgc atgacggtat tccgagcttg
    28561 ccagcagtac gcccgccaga aacgcgccca tcgccatcga caagccgacc tcttccagca
    28621 gcaaaccaaa gccaaacaca aggaataacg ccacggcact aaacacttcc cgcaagccag
    28681 agcgggcaac aaaacgcagc gccggacgcg taacatagcg ccccagcaat accaccagta
    28741 ccagcgcacc cgccactttt aacgccgaga gagcaaatgc gcccatcgtc gtcgaggcac
    28801 tgctcgccgc cagtagcggg atcatcgcca ccagcgggat tgccgcgata tcctggaaca
    28861 gcagcactgc aaaggcactg cggcccattt gcgtcaccat cagattgcgt tcattcatcg
    28921 cctgcatggc aatcgccgtt gatgagagcg ccagcgtcat accgatcaat tccgcgacct
    28981 gccagcgcag cccaagtaac atacagaaca gccccagcag gccaccgcaa atcaccatct
    29041 gcaatgcgcc accgccgaac actgccgcac gcagcttcca cagcctttgt ggatcgagtt
    29101 cgaggccgat aataaacagc atcagcacca ccccaatctc ggcaaagtgc agaatagatt
    29161 cggcatcggt caccagtcgc agcccccacg ggccaataat gcagccggcg atcaggtagc
    29221 caagtaccga tcccagacca agacgtaccg caatgggtac aatcagcgcc gccgatccga
    29281 ggtagatcag cgcctgaacc agcgtatgac tatccatgat gggcctcctg ccattccagc
    29341 agacgttgct tatagtggcg cgcctgccct tcgagggttt cgtcgtcaca aataaaggtg
    29401 cagtgcatgg caaacggtgg cagccagttc agcccgcagt agattgccgt cgcctgtagc
    29461 ggctgcgaca gcacatcaaa gcccggatgc gcaccaattt caaaatggct ttccccgccg
    29521 ccggtcgtca ccgcccacag caaatgtttg ccatgcagcg ccgtgccgcc gtgaccgtaa
    29581 gcccagccgt gcgacaaaac tttatcgatc caaagtttaa gaagcggagg aatgctgtac
    29641 cactgcatcg ggtgctgcca gacgatcaga tcggcgcgag agagcgcctc ctgctcggcg
    29701 gcaatatcga tattgaagtc aggatagagt tgataaagag agcgaatttc gacgccttcc
    29761 agcgtccttg cctgttcaag catccgttta ttcgcatggg aatgatgcgg atacggatgc
    29821 gcataaatga tgaggatcat agttattagc ccgcgtgttt acgcctttgt tttcccacaa
    29881 agtgtagtca tatattcacg cggcttatag tcaataatat tgattcagaa aagagagaaa
    29941 tctgaacaaa taatcccatt ctctgcccgc ggatcgtcat attgctggtc tgcgccagtg
    30001 acattcctcg agtctccggg gcaaacgcta cggaaatcaa caagccaaac agcgagatac
    30061 ccgcccccat tagcatcgtg ttactgatac cgtaattatt gataaatatc ggcagtgccc
    30121 aggtcgaaac aatggtgcca atacgactta aggacataat cacgcccacg gcagaggcgc
    30181 ggatatccgt cgggaagagt tcattaggat agagccactg caaattaccc ggcccgccag
    30241 agaaaaaggc atacaccgca aaggccatca ctaccagcca gatccccata tccgggatta
    30301 gccccaaaac cgccagcgcc agcgtcatca tggcaaagct gccaatcaac aatggacgcc
    30361 gtccggcagt gtttagccac agcatcggcg gaatacagcc gagcataaag aacaggctaa
    30421 tcaccacatt ccctagtgcc gcgtttttgc caacccccaa tcccaacgaa ccaacgattt
    30481 gcgggccaaa ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag atggcggcaa
    30541 caaacagaac aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg gtttgctgcg
    30601 gctgctcttc atcgaaagcc accggttcgc caaacagttt tatcatcatc tcttcgcact
    30661 cttttactcg ccctttgcgt aataaccagc ggggagattc tggcagttcg aatcgaccaa
    30721 tcaaaatcaa caaacaaggg atcgccgcgc tacccagcat ccagcgccaa ccaccttcca
    30781 catcataaag ccagtagccg accagatcgg cgcaggtcgc gccgacatac cacatcgcgg
    30841 caataaagct gatggaaaac gcccgctgac gggtactgga gaactcggtg atcatcgaag
    30901 tggcgatggg ataatctgca ccgatgacaa tgccgatgag tacccgcatc accaacagtt
    30961 cgacggggga tgaaacacac atcgtcgcca ccgatatcac gccgatggcg atgatatcaa
    31021 tgaggaacat tttgcgccgt ccgactttat cggagatata gccaaacagc gatgtgccaa
    31081 cgaacagccc ggcgagcgtt cccgcgccca gcaagccaat ccagtcagcg tccagtttca
    31141 gcgccggagt cagttgctcc agcgccacgc caatcattac cagtacataa ccatccagaa
    31201 acggaccacc gcttccccac agcaaaatgc ggcggtgaat agaggagaat ttgagatcgt
    31261 caaagtttct ggacggttgc atgggttatt cctgtttttt tgcaatgaga gtcagtacgg
    31321 cgttacgtat caaaccaaca tcagccgtaa cggaactcca caccaaaggt gccgcgcggg
    31381 tattcccact gttccagcgc cgaccccagc cccagaatgc gacaggtgcc gcactccaga
    31441 catccggcgt aatcgaagcg cacactgccg tcatcctgct tcttgtacag acctgcgggg
    31501 cacgctttca ccagcagctc cagcacctgt ttatcagcat cggccttcac aacaatgtgc
    31561 ggatgctctt catcgacatt gaatttattg acgcccagtt tgacgtccac attgacggga
    31621 gaagtcataa aacggtcact cctttcatgc catccttgat cagattgatg aagcccactt
    31681 tcttgccgtg gcggaggatt ttcttgcgca tcagttccgg cgcactgccg tcaatggtga
    31741 acaggtcacg cgccacgccc accgccagtt ccgggtagcc gctaaacatg cgtgggttat
    31801 caaggaacgc cggtagtttc tggtacatgc gcatatcgcg cagcgggctg ctctccagat
    31861 gctgacgata ttccgcgagt ttttgcttac tgaaatcgtc gcttttcatc gctgaaagca
    31921 cggtttttgc tgcagcttcc ccggcggcaa tcgccagatc cataccgcga atggtaaaac
    31981 cgaggttcat acacattccg gcggcatcac cggcaatcaa tacgccgtca ccgaccagct
    32041 ccggcagcat gttgatgcct gcttccggca ctacgtgagc ggaatattcc accagcttgc
    32101 cgcccgcgat cagcggtgca acggccggat gctgtttgaa atcttccagc atttgcggca
    32161 ccgatttttt cgcgtcatgc agatgatgca gaccacaaac cagccccagc gacagggtat
    32221 tttcattggt ataaaggaag ccgccgccca tcaggccatc ggtgggtgat cccgcaaaca
    32281 gacaagccgc gccctgatta ccctgcaact gaaaacggtc ttcgattacc gacttcggta
    32341 actcgatcag ttccttcacg ccaaccgcca catccgtcgg tttaacgcgt tttgccatcc
    32401 ccaacttttc ggcaaggatg gagttcaccc catcagcaag gagcaccgtt ttcgcttcaa
    32461 tcacatcgcc atcggcttct acaccgacga ctttgccatc gcgctgtacg aggttatcga
    32521 cgcggatccc ggtaattaac tgcgcgcccg cttcttcggc ctgctccatc aaccaggcat
    32581 caaatttact gcgcaaaacg gagtaagaac gctgggatag cgaggtttcg tcaccgttgc
    32641 agtagtccat agtcatcgct gacttttccg tcataaacgc gagtttttca tgggtgatca
    32701 ggcgttctac gggggcggaa tcggcgaaac ctggaataat gtgttccaga ctgtgggcat
    32761 agagacgccc gccagtaacg ttcttggcac ccgcggaatt gccacgctcg ataactaaca
    32821 cttgcgcccc ttcgcgggcg agcaccagtg cggcaaccga accggcaagc cctgcaccga
    32881 cgatgatggc gtcaaagata tcttcggaca taagagctcc ctgtcagcgg cagagccgta
    32941 atgcagccct gccagagtgg atcaacgcgc taaagctgcg gtcagtgccg gaaggatctt
    33001 cacggcatcg ccaacaatgc cgtaatccgc gtactggaag atcggcgcat ttttatcttt
    33061 attgatggcg aaaatggttt gcgacgcgtt agcgccaacc atgtgctgga tctgcccgga
    33121 gatccccacc gccaggtaca gttcaggttt cagcatcagg ttggagatac cgacatagcg
    33181 ttcgtgctcc atccattttt cgttttctgc caccggacga gaacaggcca actccgcacc
    33241 tatcgccttg caaagctgtt ctgccagtgc aatgttctct ttgctgccaa tgccgcgacc
    33301 gacgctgacc accagacggg ctttgtcgag atcgacgctg ttgctctggc gcgcctgggt
    33361 tgccgtgcgg gtaatcgcca cagccggagc ctgccactcc acggtgtgcg tttcgccagt
    33421 gcgtgacgcg tctggctgag ccgcatcgaa cgtgccgctg ctgatggtca gtaccgcata
    33481 cggcgtggca atgcgttctt cgccaatcgc cagaccgccg taaaccatgt gtttcactgt
    33541 tgctttaccg tcctgtacgc tgacggtgct ggcatcgtta gacaccgccg ctttaaggcg
    33601 ataacccagt tttgccgcca gtaatttgcc gcgacgggtg tttggcaaca gcaccagacc
    33661 gtcagcgccg tgctggcgaa tagtgtcagc catgacaccg gcgtaatctt cgatcatccg
    33721 atcgtccggt ttgccgttta atttccagac atgattagcg ccgagctgga ttgcctgtgc
    33781 gccgtcggca tcattgagga caaaggcatt gatttgatta gctaaagcct gcgcaccgtt
    33841 catcagttcc ggcagacgag agggggtatc gctgaatacc cagacttgag aaaacgtgtt
    33901 catagcatcc cctgtaatta aatgactttg cgaagatttt cagcaaatgc ggcgatctgt
    33961 tcttcgccgt cgccttcaat cacgatgcgc tgacgctcgc gctgtttcgg cgcggcaacc
    34021 tgttgttcag accaggcgtt cacggcgtta aaaccaatat ccgccgccga ccatacctgg
    34081 actggctttt tcgccgcgcc gagaatggct ttcatcgaag gaatttgtgg ggagttgata
    34141 tcagtggaaa cagcaacaac cgcaggcagc ggaatgctta aggtttcggt ttcatcttcc
    34201 agttcgcgct caacggtgag ggtatctgcc gtcagggaga taattttgct gacgccgtta
    34261 actgccggaa tattgaggat ttcgcccacc agcagaccaa cctgctgggc ataaaggtcg
    34321 gaagaaccat cgccacagag gatcagatca aagcctgctt tctgggcggc tgcagccagt
    34381 gcgctcgccg tttgttgcgg cagtgcctgc tcgaactggt catcaatcac cacaatcagt
    34441 tcatccgggc cgcgcgatag cacatcttta cgccctttgg cgttggtcag ggctttaccg
    34501 cccacactta aggctgtcac ctgcgcctct gctgcctgtt gctttagctg gcaagccgct
    34561 tcaatagcgt tgagatcgta ttggcttatt ttggcatcgg ctttgctgaa gtctaatgaa
    34621 ccatcagcat tattgaccgc aatatcctgt tcatcaggca cgcacttata gcaagtaata
    34681 atcttcattg catctccaga aatcatgaag gaaacgtcgt tcatatcggt agtgttaatt
    34741 acagatatca cgccctttaa tttggaaata aaactttaat caccaatatt gaaaatgtca
    34801 cacgcagata aatccaactt tcaatattgt taagttcctc accaatattg aaaacatggc
    34861 gtagcaaaaa gaaattttca atattgtttt atagatcacc aatattgaaa gtcagatgct
    34921 attttttacc cacaaaagaa cataacaaac tgattaataa ccaaaattaa cgaaaaacgt
    34981 cctctattca ctcgaaacaa aaatgtgaca ccaatcacag aatacagctt attgaatacc
    35041 cattatgagt tagccattaa cgcgtccacg aggttaataa taattatatt aaatgttaac
    35101 aaaaataaaa caaacgggaa cacaaaataa atattcgttt tcacagtgga attaactcat
    35161 gaagaatgaa aagagaaaaa cgggaataga accgaaggtt ttctttccgc cattaataat
    35221 cgtcggcata ctttgttggc ttacagtcag agatctggat gcggcgaatg tcgttattaa
    35281 tgctgtattc agttacgtca ccaatgtatg gggatgggca tttgaatggt atatggtggt
    35341 gatgcttttc ggttggttct ggctggtgtt tggcccgtat gccaaaaagc gtttaggtaa
    35401 cgaaccgcca gaatttagca ccgccagttg gatctttatg atgttcgcct cctgtacgtc
    35461 tgctgccgta ctgttctggg gatcgattga gatctactac tacatctcca ccccgccgtt
    35521 tggcttagaa ccgaactcga caggagcgaa agagttgggg ctggcttaca gcttgttcca
    35581 ctggggacct ctgccgtggg ccacttacag cttcctttca gtcgccttcg cttacttctt
    35641 ctttgtccgc aaaatggaag tgattcgccc cagctcgaca ctggtgccgc tggtaggtga
    35701 aaaacacgcc aaagggttgt tcggtaccat cgtcgacaac ttctatctcg tcgccttgat
    35761 cttcgcgatg ggtaccagtc tgggccttgc cacgccgctg gtgaccgagt gtatgcaatg
    35821 gttgtttgac attccgcata ccctgcaact ggacgctatc atcattacct gctggattat
    35881 cctcaacgcc atttgcgtcg cttgcggtct gcaaaaaggg gtacgtatcg ccagtgacgt
    35941 gcgtagttac ctgagcttcc tgatgctggg ttgggtgttc atcgtcagcg gtgccagctt
    36001 catcatgaac tacttcaccg attcggtggg gatgttgctg atgtatctgc cgcgcatgtt
    36061 gttctatacc gatcccatcg ctaaaggcgg cttcccgcag ggctggaccg tgttctactg
    36121 ggcatggtgg gtgatttacg ccatccagat gagtatcttc ctcgcccgca tctcccgtgg
    36181 tcgtactgtg cgtgaactgt gcttcggcat ggtgatgggg ctgacagcat ccacctggat
    36241 cctgtggacc gtgctcggta gtaacactct gctgttgatg gataaaaaca tcatcaacat
    36301 tccaaatctg atcgaacagt acggtgtggc gcgcgccatc atcgaaacct gggccgctct
    36361 gccactcagc accgccacca tgtggggctt cttcatcctc tgctttattg ccaccgttac
    36421 gctggttaac gcctgctctt ataccctggc gatgtctact tgccgcgaag tacgcgatgg
    36481 tgaagaacca ccgctgctgg tgcgtatcgg ttggtcaatt ctggttggca ttatcggtat
    36541 tgttctgctg gcgctcggcg gcctgaaacc gattcaaacc gccattatcg ccggaggatg
    36601 cccgctgttc ttcgtcaaca ttatggtgac gctctccttt attaaagacg cgaaacagaa
    36661 ctggaaagat taattaaccc ccaaaatatc aagaggttga aagatggatt ttaatttaaa
    36721 tgatgagcag gaactgtttg tcgccggtat ccgcgaactg atggccagcg aaaactggga
    36781 ggcctatttt gccgagtgcg accgtgacag cgtctacccg gaacgttttg tcaaagcact
    36841 ggcggatatg ggtatcgaca gtctgctgat ccctgaagag cacggtggtc tggacgcggg
    36901 gtttgttact ctcgccgccg tgtggatgga gctgggacgt ctgggggcac caacctatgt
    36961 actgtaccag ctgccgggcg ggttcaatac cttcctgcgc gaaggcacac aagagcagat
    37021 cgacaagatt atggctttcc gcggcaccgg taagcagatg tggaactcag cgattaccga
    37081 accgggcgcg ggctccgacg tgggtagcct gaaaacgact tatacccgta gaaatggtaa
    37141 gatttatctt aatggtagta agtgttttat taccagtagc gcctacaccc cgtacatcgt
    37201 ggtgatggcg cgcgacgggg cttctccgga caaacctgtc tacaccgaat ggtttgttga
    37261 tatgagcaaa ccgggcatca aagtgaccaa acttgagaag ctcggtctgc gtatggatag
    37321 ctgctgtgaa atcacctttg acgatgtgga actggacgag aaagacatgt tcggtcggga
    37381 aggtaacggc tttaaccgcg tcaaagaaga gttcgaccat gaacgtttcc tggtagccct
    37441 gaccaactac ggtacggcga tgtgcgcctt tgaagatgcg gcgcgctacg ccaaccagcg
    37501 cgtgcagttt ggcgaggcta ttggtcgttt ccagttgatt caggaaaaat tcgcccacat
    37561 ggcgatcaaa ttaaactcca tgaaaaacat gctgtatgaa gcagcgtgga aagcagacaa
    37621 cggcaccatc acctctggcg atgcagcgat gtgcaaatac ttctgcgcca atgcggcatt
    37681 tgaagtggtg gatagcgcaa tgcaggtgct gggcggtgtc gggattgcgg gcaaccaccg
    37741 catcagccgc ttctggcgtg acctgcgtgt agaccgcgtt tccggaggat ctgacgaaat
    37801 gcagatcctg acgctgggtc gtgcggtgct gaagcaatac cgctaagtcc gtgcgctgcc
    37861 tgatgcgacg ctgacgcgtc ttatcaggcc tacgggagca cgaatgtaag ccggataagg
    37921 cgtttacgcc gcatccggca gtcatgcgct acataaattt ttcaggagct aataatggat
    37981 catctaccca tgccgaaatt cggaccgctg gccggattgc gcgttgtctt ctccggtatc
    38041 gaaatcgccg gaccgtttgc cggacaaatg ttcgcagaat ggggcgcgga agttatttgg
    38101 atcgagaacg tcgcctgggc cgacaccatt cgcgttcaac cgaactaccc gcagctctcc
    38161 cgccgcaatt tgcacgcgct gtcgttaaat attttcaaag atgaaggccg cgaagcgttt
    38221 ctgaaattaa tggaaaccac cgatatcttc atcgaagcca gtaaaggtcc ggcctttgcc
    38281 cgtcgtggca ttaccgatga agtactgtgg cagcacaacc cgaaactggt tatcgctcac
    38341 ctgtccggtt ttggtcagta cggcaccgag gagtacacca atcttccggc ctataacacc
    38401 atcgcccagg ccttcagtgg ttacctgatt cagaacggtg atgttgacca gccaatgcct
    38461 gccttcccgt ataccgccga ttacttttct ggcctgaccg ccaccacggc ggcgctggca
    38521 gcactgcata aagtgcgtga aaccggtaaa ggcgaaagta tcgacatcgc catgtatgaa
    38581 gtgatgctgc gtatgggcca gtacttcatg atggattact tcaacggcgg cgaaatgtgc
    38641 ccgcgcatga gcaaaggtaa agatccctac tacgccggtt gcggtctgta taaatgtgcc
    38701 ggcggctaca tcgtgatgga actggtgggc attacccaaa ttgaagagtg ctttaaagat
    38761 attggcctcg cacatctact cggtacgccg gaaatcccgg aaggcactca gcttatccac
    38821 cgtatcgaat gcccttacgg cccactggta gaagaaaaac tcgatgcctg gctggcggca
    38881 cataccatcg cagaagttaa agaacgcttt gccgaactga atatcgcctg tgccaaagta
    38941 ctgaccgtac cggaactgga aagcaatcca cagtatgttg cccgtgaatc aatcactcag
    39001 tggcaaacga tggatggtcg cacctgcaaa gggccgaaca tcatgccgaa attcaaaaat
    39061 aaccccggac aaatctggcg cggaatgccc tcacatggca tggacacggc tgccattttg
    39121 aaaaatatcg gctacagcga aaacgacatt caggagttgg tcagcaaagg tctggccaaa
    39181 gttgaggact aaacgcgtgg cgtcagtcgg cttgcctgac tagcgcccgc ccaataacaa
    39241 acgaacaaaa atggatagag gtgcaatgga tatcattggc ggacaacatc tacgtcaaat
    39301 gtgggacgat ctggcggacg tttacggtca taaaacggcg ctgatttgtg aatccagcgg
    39361 cggagtcgtt aaccggtata gttatcttga gttaaatcag gagattaacc gcacggcaaa
    39421 cctgttttat acgctgggga ttcgcaaagg caacaaggtt gcactacatc tcgacaactg
    39481 cccggaattt atcttttgct ggttcgggct ggcaaaaatt ggcgcgatta tggtgccgat
    39541 taacgcccgc ctgttacgcg aggaaagcga gtggatcttg caaaatagcc aggcgtgcct
    39601 gctggtgacc agtgcgcaat tttatcctat gtatcaacag attcagcagg aagatgccac
    39661 tcaattgcgg cacatttgcc tgacagatgt ggcacttccc gctgatgatg gagtgagttc
    39721 atttactcaa ctgaaaaatc aacaacctgc caccttgtgc tatgcaccgc cgctatcgac
    39781 tgacgatacg gcggaaattc tcttcacctc cggcaccacc tcccgaccga aaggtgtggt
    39841 gattacccat tacaacctgc gcttcgctgg atattactcc gcctggcagt gtgcactgcg
    39901 tgacgatgac gtctacatga cggtaatgcc tgcgtttcat atcgattgcc agtgtactgc
    39961 ggcgatggcg gcgttttctg ccggggccac ctttgtgctg gtcgagaaat acagcgcccg
    40021 cgccttctgg ggacaggcgc agaagtaccg cgccaccatt accgaatgta ttccgatgat
    40081 gatccgtacg ttgatggtgc agccgccttc agcgaacgat cggcaacacc gcctgcggga
    40141 agtgatgttt tatctcaact tgtcggagca ggaaaaagac gcattttgtg aacgcttcgg
    40201 cgttcgcttg ctgacgtctt atgggatgac ggaaaccatt gtgggcatta tcggcgatcg
    40261 ccctagcgat aaacgacgct ggccgtcgat tggtcgggcg gggttttgct acgaagcgga
    40321 gatccgcgac gatcacaatc gcccgctccc ggcaggtgag atcggtgaaa tctgtattaa
    40381 aggcgtacca gggaaaacca tcttcaaaga gtattttctc aacccgaaag ccactgcgaa
    40441 agtgctggaa gccgacggct ggctacatac cggcgatacc ggataccgcg acgaagaagg
    40501 ctttttttat ttcgtcgatc gccgctgcaa tatgatcaaa cgcggtggcg agaatgtctc
    40561 ctgcgtggag ctggaaaata tcatcgccac ccatccgaaa attcaggaca tcgtggttgt
    40621 gggtattaaa gattcgattc gcgatgaagc catcaaagca tttgtggtgc tgaatgaagg
    40681 tgaaacattg agcgaagagg aattttttcg cttctgcgaa caaaatatgg cgaaatttaa
    40741 agtgccctct tatctggaga tcagaaaaga tctgccacgt aattgctcgg ggaaaataat
    40801 tagaaagaat ctgaaataaa tgaaacgtca gggaactacc ctgccagcta ataatcacgc
    40861 cctaaaacaa tatgcatttt ttgcagggat gttgtcgtcc ctaaaaaagc aaaaatggag
    40921 aaaaggaatg agtgaatcat tacatctgac ccgcaatgga tcaattctgg aaattaccct
    40981 tgatcgccca aaagcgaatg ctattgatgc aaaaaccagc tttgaaatgg gcgaagtatt
    41041 tctaaatttc cgtgacgatc cgcaattacg tgtcgccatt attaccggtg ccggagagaa
    41101 attcttttcc gcgggctggg atttaaaagc ggcagcagaa ggcgaagcac cggatgctga
    41161 ctttggcccg ggtggttttg cgggattaac cgaaattttc aatctcgaca aaccagttat
    41221 cgcagctgtg aacggctacg cctttggcgg cggtttcgaa ctggcgctgg cggcagattt
    41281 tattgtttgt gccgataacg ccagcttcgc cctgccggaa gccaaactgg gcatcgttcc
    41341 tgacagcggc ggtgtgctgc gtctgccgaa gatcctgccg cctaccatcg tcaatgaaat
    41401 ggtgatgacc ggcagacgaa tgggcgcaga agaggcgctg cgttggggga tagtcaaccg
    41461 cgtggttagc caggcggaac tgatggataa cgcccgcgaa ctggctcagc agctggttaa
    41521 cagcgccccg ctggcgattg cggcgctgaa agagatctac cgcaccacca gcgaaatgcc
    41581 ggtagaagaa gcgtatcgct atattcgcag cggcgtgttg aaacactatc catcggttct
    41641 gcattcggaa gatgccattg aagggccgct ggcgtttgcc gagaagcgcg atccggtgtg
    41701 gaaaggacgt taacaaccgt gagctattac gcctttgagg ggttaattcc ggtggttcac
    41761 ccgacggcgt ttgtccatcc cagtgccgtc ttgattggcg atgtgattgt gggagccggt
    41821 gtctacatcg gcccactcgc ctcactgcgt ggtgactacg ggcggttgat cgtgcaagcg
    41881 ggagccaata ttcaggatgg ctgcattatg catggctact gcgacactga caccatcgtt
    41941 ggggaaaacg gccatatcgg gcatggagcg atcctgcatg gttgtgtgat tggtcgcgat
    42001 gcattggtcg ggatgaacag cgtaattatg gatggcgcgg tcattggcga agagagcatt
    42061 gttgccgcaa tgagctttgt caaagcaggc tttagtggcg agaaacgcca gttgttgatg
    42121 ggtacgccag cccgcgctgt acgcagtgtt agtgacgacg agttacactg gaaacggttg
    42181 aataccaaag agtatcagga tcttgtcggg cgctgtcatg catcgttaca tgaaacgcag
    42241 ccactgaggc aaatggagga aaatcgcccc cgtttgcagg ggacgacgga tgtgacgcct
    42301 aaacggtgat cttgccggat gcgacctggt tgctggataa gatgctacaa catcgtatct
    42361 ggcaagcagg acgcccggta tcaacgagat aaaattaacg acgcatactc tttgacaaca
    42421 tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg aacggcagga atacgcggtg
    42481 tctttcttac gccgactaat ttttcccgac tgttattgcg caggcgcgcg taaatttgct
    42541 catcgatatc gaccacttta accagtcgct gacactggca tccccgccct tccagcttat
    42601 tagggatcat cttaacttcg cagcttattt ctgtgacttc cgacagaata taagtcaggg
    42661 tattaaccgc cttgctgtgt tcaatatcga aatgaataga gatctctctt gctatcaccc
    42721 accgattttc agccatcatc cattcggcga ttaacaagta gagtggtttt tcaacatatc
    42781 cttcacacat acgaactcca gtttcacaaa ttaaaagtct ggctatgact cgttttgaag
    42841 caattaaaca agaccatatt aaaattgtgg atatatcgat agtatgcaac ttcaccgtcg
    42901 ataagtgtga gctaaatcct gcttatgtca tcaaaaatat cgattcccca aaagatttac
    42961 ttaatgggca aaaaaaaacg gtcctcatca gagaaccgta ttagttattc gatcaaatta
    43021 gcggatgaaa aatatccgcc acgacacgct attatttgat ctgtgcgtgc atttcctgca
    43081 ccgaaattac tttttcagtc gcatcggcat tcagcgccat cgcggtagcg aaaccaccgt
    43141 tcagggtggt gtcataatgc actttatatt gcagcgcact gcgacggatc acgcgggagt
    43201 cttcaatcgc acgacggcct gaggtggtgt tgatgatgta ggtatattcg ccattcttga
    43261 tacggtcctg aatgtgcgga cggccttcat gcaccttgtt taccagacgc ggattgatac
    43321 ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag ccctgtttca
    43381 gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg gaaagcagcg
    43441 cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggcg aacgcttcag
    43501 cgaaggtgcg gcccacgccc atgacttccc cggtagagcg catttctggc cctaacagcg
    43561 ggtcaacgcc tgggaattta ttgaacggca gcaccacttc tttcaccgag tagtacggcg
    43621 gaataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc acacgcgccg
    43681 ccacttttgc cagcggtacg ccggtggctt tggagacgaa cggaacagta cgcgccgcac
    43741 gcgggtttac ttcaatcagg taaacttcgt tgtttttcac cgcaaactgc acgttcatca
    43801 gaccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc acatcctgaa
    43861 tttcctgact taaggtgtac gctggcagcg aacatgcgga gtcaccggag tgcacgcccg
    43921 cctgctcgat gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg cagatggcat
    43981 ccacgtcaac ttctactgcg tcatcaagga aatgatccag caacactggc gcatcgttag
    44041 acacgctaac cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag acgatttcca
    44101 tcgcccgacc gccgagaacg taagacggac gcaccaccag cgggtagcca atctcttttg
    44161 ccttctcaac tgccatttca atagcagtaa cggtggcgtt cgccggttgt ttcagtttca
    44221 gacggtcaac cgcatgctgg aagcgttcac ggtcttccgc acggtcgata gcatccgggc
    44281 tggtgccgat aaccggtacg ccagcggctt ccagtgcacg cgccagtttc agcggggttt
    44341 gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt tccagcacat
    44401 cttccagagt taccggctcg aagtagaggc gatcggaagt gtcgtagtcg gtagagacgg
    44461 tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcacgcagc gccagcgagg
    44521 cgtgtacgca gcagtagtcg aattcgatac cctgaccgat acggtttgga ccgccgccaa
    44581 gcaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct tcataagtgg
    44641 agtacatgta agcggtgtcg gtggcaaact ctgccgcaca ggtatccacg cgcttgtaga
    44701 ccgggtgcag gtcatattgg tcacgcagct tacggatttc cgcttcgcgt acgcccgcca
    44761 gttttgccaa gcgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg aattcagcgt
    44821 tcaggccagt gatgcccact tccgccactt tctcttccag acgcaccagt tcttcaatct
    44881 gtaccaggaa ccagcggtca atgttagtca ggttgaagac gccgtccaca gacaggcccg
    44941 cgcggaaagc atcggcgatg taccagatac gctctgcgcc tgcgtctttc agttcgcgac
    45001 ggattttggt taacgcttcc gggtcatcca ggctcacttt cgggtcgaag ccagttgcgc
    45061 ccacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga ccaatcgcca
    45121 tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcaccg gcgaattttt
    45181 cgaagttgaa gcgaggaatt ttagtaacca catagtcgat ggacggctcg aaggaggccg
    45241 gagtacgtcc gccagtgatg tcgttcatca gttcgtctag ggtgtaaccc accgccagtt
    45301 tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc gaagaacggg
    45361 acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt tttcgggttc accgcaaact
    45421 ggacattgga accaccggtt tcaacgccga tttcacgcag caccgccatc gaggcgttac
    45481 gcatgatttg atattctttg tcagtcagcg tttgggctgg cgcgacagtg atggagtcac
    45541 cggtgtggat gcccatcgca tcaaagtttt cgatagagca gacgatgatg cagttgtcgt
    45601 ttttatcacg caccacttcc atctcgtact ctttccagcc gatcagcgac tcatcaatca
    45661 gcaactcttt ggtcggagag agatccagac cgcgggcgca aatttcttca aactcttcac
    45721 ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga ataatgcacg
    45781 ggaagcccac gtcagcggca acagccagcg cttcttccat cgtgtgtgcg ataccggaac
    45841 gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg tcttctgctt
    45901 tatcaatcgc atcggcagtg gcaccaatca tggtgacacc gaactcttcc aacacgccct
    45961 gacgttccag ctccagcgcg cagttcagcg ccgtctgacc gcccatggtt ggcagcaccg
    46021 cgtccgggcg ctctttttca ataatcttgc gtaccacttc ccagtgaatc ggctcgatgt
    46081 aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc cgggttggag ttcaccagaa
    46141 tgacgcggta accctcttcg cgcagggctt tacatgcttg cgcgccagag tagtcaaact
    46201 cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt tttatatctg
    46261 tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt actgctcaat
    46321 taacttgata aagtggtcga acaacggcgc ggcgtcgtgt ggaccagggc tggcttcagg
    46381 gtgaccctgg aagctgaacg ccggtttatc gatacgatga atgccctgta acgtaccgtc
    46441 gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat ccaccgcaaa
    46501 accgtggttc tgggcggtga tcatcaccac gtttttctcc acatctttaa ccggatggtt
    46561 gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca gcgccagcag
    46621 ctgatgaccg agacagatgc cgaataccgg aatatcggtt tcgaggaact tctggatggc
    46681 ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga agatgccgtc
    46741 tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg tcaggcgaca
    46801 gcctctatcc accagcatcc gcaggatgtt gcgcttggca ccaaaatcat aagccacgac
    46861 gtggaacggc agctcgtctt cttttttcgc ttctggcagg ccgccggtca acgtccagct
    46921 cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat ccatgccgtt
    46981 cagacctggg aacgcgcggg ctttttctaa cgccagcgcc gcatccgggt tatcgcctgc
    47041 gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct tacgggtatc
    47101 gatatcggca atcgccacga tgttatggcg cttcaggtaa gaagagaggt cttcggtatt
    47161 acggaagttg ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg catgtacctg
    47221 agaagattct tcatcggcgt cattagtgcc gacattgcca atatggggat aagtaagagt
    47281 aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg tcattgaagt
    47341 attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac cgtgaaactg
    47401 ggttccgtct tccagaacca atagcgctga cttaatcaaa acacctccat agaatattca
    47461 ctcactttat ttgcatatta attcacagtg atgacgtaaa tcaatgcaaa tctgcttacc
    47521 gacgaatttc tggcaaacgg cggcattctg gagatatggg cgctaaaagt caacttaatg
    47581 gcaggatttt ttatattatt ttgtggctca tgcatgtttt acaggcatta agcgacaaaa
    47641 acatcatcta acctgttctg gaaaacgctt gcgcagcaag aagattgcat gaaataaaaa
    47701 cgagcaggaa aaagtggacc aaatggtcaa aatttacatt aaaacaacaa aaatagaaga
    47761 cgaaacatgt aatttttggc agttatagaa aatttaaaag cataacacca aaaataaaag
    47821 ggctaaaaat taaagccctt tataattaat aaattaaatg tgttactttt gcatcataat
    47881 aaacattttg tggttacaaa ttattgagat caagcacatc tcgcatatca aaaagaccac
    47941 tttccttacc actcaaccac aaagccgatc ttaccgcacc gttagcaaat gtcatgcggc
    48001 tggacgcctt atgggtgatc tccagacgct cgccaatatc ggcaaacatc gcggtatgtt
    48061 cgccaacgat gtcacctgca cgcacggtgg caaaaccaat ggtaccaggc acacgttcac
    48121 cggtatggcc ttcacgactg tagaccgcgc aatctttcag atctttatca agggcgtggg
    48181 cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc atcaacttta tgtctatgat
    48241 gtgcttcaat aatttcgata tcggtgtagt cacccatcac tttggctgct ttctccagca
    48301 gcttaagcat gacgttaacg ccaacgctaa aattggcggc aaagacaatc gcaatatcgg
    48361 cggcggcgtc acgaattgcc tgtttaccgg cttcgtcaaa ccccgtagtg ccgatcacca
    48421 tccctttgcc atgctgccga caaaaagcga gatggttcag cgtaccttcc ggacgggtaa
    48481 aatcgataaa cacatcaaaa tcatctttta tcgcatcaag actgctttgc acggtaacgc
    48541 ctgttttccc tgcaccagcc agctcaccgg cgtcgctgcc cagtaaagaa gatccttcgc
    48601 gctccagcgc cgcgcccagc tgcacgcctt ctaatgccag tgccgcctga atcaactggc
    48661 ggcccatacg ccccccggct cccgcgatgg caacgcggat gtttgcatca tgcatagcta
    48721 ttctcttttg ttaatttgca tagaccgttt tcagagtaac cagcgcatcg ctacgccgcc
    48781 agccaaaaac gctgataatt agaaattaat gataaacagg gagggatatc agtgaaaggc
    48841 atgagtcgat gaatgactgc atgccgataa catgtgacag gttacggcac cagagccagc
    48901 acctcagcca cccactgttg gaagcctttt acattcagat ccaatgccac ctgtacattg
    48961 gctggtttgc ccaggcaacc gtcgatatca accaccgtcg tgcctgaggt aaattcgcct
    49021 tgagtttcta ccgcgacaaa acacggcttg agggtgaata agtccgggcg caccagccag
    49081 gcgatggcgc agagatcgtg cattcgcaga ccgctttgca tactgccgct acggtagtgg
    49141 ctaaacaggg cgtggagcat ttttccggtg cgattcaact ccggtaaagt agcgagatag
    49201 tcaggggtta atattgcctg attggtgaca tccagaccgc acatgacaat ttcgatgccg
    49261 ctacggaaga cacaggcagc cgcttctgga tcggcagcga tattaaactc ggcatttggt
    49321 gtacagttgc cgcgaccagc agaaccgccc atgatcacca gacgacgaat atacggttta
    49381 cattccgggc attgtaaaag taacagcgca atattggtta acgggccgat ggccaccagg
    49441 gtaacgggct ctggtgcacg catcagggca tcccgaatcg ccagaaacgc cggtatcccg
    49501 agtggctttc ggttgtgctc aacaaagtcg tagccagcca ttcccgattc gccgtgcaca
    49561 gatgctgcat cacgcggtgc gcgtaccagc ggcacagcgg ctccctgggc gagcggaatc
    49621 tccgcattcc agaaatgcag cagttgcagg gcattgcggg tagttttctc aaccgagaca
    49681 ttacccgcga cggtggtcat cagttgcagg tcgagttcgg gggcaaaaat cgcggcggca
    49741 atggcgacgg catcgtcaat gccggggtca gtatcgagga agataggtaa acgcatgttt
    49801 tcttcataaa aaatgccggt aacaagaccg gcattttcgc ataacttagg ctgctaatga
    49861 cttaatcgac ttcacgaata tcgacacgca gctctttcgg cacttcgaaa acaatatttt
    49921 cttcacggcc ttccagcgga atggcttcac caccacccaa ctgctgcaaa cgtgccacca
    49981 cattctgcac cagaatatcc ggagccgatg cgcccgcagt cacgccgacg catttaacct
    50041 ctttcaccca ctcttcctgg atatctttcg catcgtcaat caaaaacgcg tgtttgccca
    50101 tacgctgggc cagctccgcc agacggttgg agttggagga gtttttcgaa ccgaccacca
    50161 acacaacttc cgcctgttct gccagggcgc gtaccgcttc ctgacggtta gtcgtggcgt
    50221 agcagatgtc atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc agcgcgtcga
    50281 tcacatcaga cgtatcatcc accgacagcg tggtttgggt cataaaggag agcttctctt
    50341 cgtttttgac cgtcagtttc cacacatcgt ctggcgattc gaccagatac attccccctt
    50401 cggggttgct gtactgcccc atcgtccctt ccacttccgg gtgcccggcg tggccgatga
    50461 gaatagattc ttcgccacgg cgactggcgc gggcaacttc catatgcact ttggtcacca
    50521 gcggacaggt ggcatcaaac accgtcaaat cgcggctttt cgcttcgtta cgaaccgcct
    50581 gagaaacacc gtgtgcggag aaaatcagga tcgcgccgtc cggcacttcg ctaatctgct
    50641 caataaagat agccccacgc tcgcgcaagc tatcgaccac gtagcggtta tgcaccactt
    50701 cgtgacggac atatatcggt gcgccgtaaa tggccagcgc gttttcaaca atgctgatag
    50761 cgcggtctac cccggcacaa aaaccacgcg ggttggccaa caggatctgc atgttacgcc
    50821 tccagtgccg gatcgatttc cagcacttca atatcaaaat gaacggtctg cccggccagc
    50881 ggatggttga aatcaacggt aatggagtcg ccgttaattt cgcggatcac accaggcatc
    50941 tcactgccat ccattgcggt aaaaagcatg attgcgccaa tttctggctc gcctgcatcc
    51001 ataaattcac ggcgggagaa gtactgaatc aggtccggtg acggcacgcc aaacgccgca
    51061 tctggctcca gcgagaaggt ggttttgtcg cccactttca gccccagcag gtgttgctcc
    51121 agcccttcag aaagagaagc atcgcccagg cggaacagcg ccggtttacc gttgttgcga
    51181 gtagactcgg cggtggtgcc atcgtcgagt tttagcgtga agtgcaccag gacggcgcta
    51241 ttgctctgta cagattcaga catgaaggtt tgctcttatt tttgaggatt tttgccggat
    51301 gcggcctacg atttcgtagc aatctgtagg ccgcatccgg cagggtttat tattgttttt
    51361 tcgctttgga aggtaaaaac ccttccagca cgatcagtgc cgcaccgaca caaatggcag
    51421 tatcggcaag gttgaaggtg gcgaagtgcc agtcgccgac gtagaagtcg atcatatcga
    51481 caacgaagcc gtgccacagg cggtcgaaca ggttgcccag cgcgccgcca ataatcagcg
    51541 cgtaagcgat attgtttagc ttctgcgtgg ccttcgagcg atacatcatc actgccagga
    51601 gcacgctaat accaatcgca ataccggcaa agaaccaacg ctgccagccg ccgctatcgg
    51661 caaggaaact aaacgccgcg ccatagttac gcgcataatg cagattaagc gacgggaaca
    51721 gcgggaccgt atcccccaga gcaaagttct ggaggatcag gtatttgctg cccagatcga
    51781 taatcagcac gactaccacc agccacagcc agcgtagccc tgttgaacag atcgattgac
    51841 tcatcaggca aacttacgtt tttcaccgtc accggcgacg ttgctgacac agcggccgca
    51901 gatttctgcg tgttccgcca ccttgccgac atcctgggtg tagtgccagc agcgtgggca
    51961 cttctcacct tcggctttac tcaacgcgac tttcagccct ttgagtactt cgctctgctg
    52021 agcatcagca ggtgcgtcgt tatagtctgc aacggtagcg ccggaggtca acaggacaaa
    52081 tcgtaattca tcgcccagcg cggtcagttt cgccgccagt tccggttctg catacaaggt
    52141 tactgccgct tccagcgagc cacccacttt cttgtcggca cgcgcttgct caatgacttt
    52201 gttcacttcg ccacgcactt tcaacagctc gtcccagaac gcatcgttca ttgcttcact
    52261 gtctgccaga ccaaacaggc cttcgtacca ctcaccggtg aagacgtatt tttcacgttc
    52321 gcccggcaga tagccccaca cttcatcagc ggtgaaggag aggattggtg ccatccagcg
    52381 caccagcgct tcagcgatgt gatacagcgc agtctggcag ctacgacgcg ccacgctatc
    52441 cgctttggcg gtgtactgac ggtctttgat gatgtcgagg tagaaggaac ccatctcaac
    52501 ggagcagaag cgcatcagac gctgtaccac ttcgtggaaa tcgtatgctt cgtacgcctt
    52561 gaggatgtct tcctgtgccg ctttcgcaca acctacggcc cagcgatcca gtaccaccat
    52621 ctcttccggt ttcaccatat cttttgctgg atcaaaacca ttcaggtttg ccagcaggaa
    52681 gcgcgcggtg ttacggatac gacgatagct atcggcagca cgtttcagga tctcgtcaga
    52741 aacggccatt tcaccggtgt agtcggttga tgccacccac agacgcagaa tatccgcgcc
    52801 cagtttgttc atcacatcct gcggcgaaac ggtgttgccg atggatttag acatcttgcg
    52861 accctgacca tccacggtaa agccgtgggt cagtacctga cgatacggcg ctttgccttt
    52921 catcgcggtg gagatcatta gggaagacat gaaccaacca cggtgctggt cagaaccttc
    52981 cagatacatg tccgctgcgt gaccggcaaa ttccggacgc acgtcaacaa cagaagagtg
    53041 ggtagatccg gagtcaaacc atacgtcgag agtatccggc actttcacat actggtcagc
    53101 ttcgtcgccg aggatctctt tcgcatcgag atcccaccac gcctggatgc cgtcaacttc
    53161 aacgcgttta gccacttctt ccatcagttc gagggtacgc ggatgcagct cttccgtgtc
    53221 tttgtgcacg aacagtgaca tcggtacgcc ccaggtgcgc tgacgggaga tacaccagtc
    53281 aggacggtta gcaaccatcg actcgatacg cgcctggccc cagtccggga tccactgcac
    53341 gcctttgatc tctttcagtg actgcgcacg cagacctttc tgatccatgc tgacgaacca
    53401 ctgcggtgtc gcgcggaaga tgatcggcgt tttgtgacgc cagcagcacg gatagctgtg
    53461 ctgcattttc tcaacgtgca gcagcgcgcc tttttcctgc agcagcgcaa cgacgatgtc
    53521 gttcgctttg aagacgttca cgccatccag cgtcggataa gtgcccggca gataagtgcc
    53581 gtccgggcca accgggttag cggtttccag gccgtatttc tgaccgatca catagtcgtc
    53641 cgggccgtgg ccaggcgcgg tgtgaacggc accggtaccg gcatccaggg taacgtgatc
    53701 gccgaggatt gccggaacgt cgaagcccat aaacggatgg gtaaagcgca gcagctcaag
    53761 ctctgcacct tttaccgtgc cgagaatggt gtaatcggtc acgccgatac gctgcattac
    53821 gctttcaacc agatctttcg ccagaatcgc ggcctgaccg tcgatctgca ccagcgcata
    53881 gtcgaaatca ggtgcaatag agattgcgcg gttcgcaggc aaagtccacg gcgtagtggt
    53941 ccagatcacc agcgagattg ggccgttaac gttgcttacg ccaaatttcg ctttcagcgc
    54001 atcctgatcg accgcctgga aagcgacgtc aatggacgga gaagttttgt cgtaatactc
    54061 aacttccgct tctgccagtg cagaacggca gtctacgcac cagtgcaccg gcttcgcgcc
    54121 tttgtgcagg tgaccattgc cgatgatttt gcccagcgcg cggatgatgt tggcttcagt
    54181 tttgaagtcc atagtcagat acgggtgcga ccagtcgccc agcacgccca gacggataaa
    54241 gtctttgcgt tgaccgtcaa cctgggtcgc cgcgtattcg cggcacttgg cgcggaactc
    54301 ggcggcggtg aatttctcac ccggcttacc gtattcttgc tctactttca gctcgatcgg
    54361 cagaccgtgg cagtcccagc caggcacata cggcgagtca taaccggaaa gccctttgga
    54421 cttcacgata atgtctttca gaatcttgtt aaccgagtga ccaatatgaa tgctgccatt
    54481 cgcataagga gggccatcat gcagaatgaa ggtttttttg ccttttttag ccgcacggat
    54541 gatgccgtac agatcatcat cagtccaacg cgccagcatt cccggttcgc gcttggcgag
    54601 atcgccacgc atcgggaacc ctgtttccgg caaattcagg gttgatttat agtcactcat
    54661 cagattctcg gttccgtgtt tcggtttgat tacataacag gcttaagccg gttttgttag
    54721 cccaaaaaat tcgcgggcgg ttaattcatc acgcgcaatc tgcgctttca gttcgtccag
    54781 cgacgcaaat cgctgctcat tgcgtatttt tttacgcagc actacttgta tatggcgacc
    54841 gtaaaggtcc attgcaacat ctaacaaatg cacttccagc tgctggcgaa taccggcaac
    54901 cgttgggcgt gttccgatgt ttgccacgcc gggtaacggc ttttcaccga ggcccagcac
    54961 ttctaccgca taaacccctt tcaccgggga aacctgacgg cgtagcggta cattcgccgt
    55021 cgggaaacct atagtgcgcc ctaattcatc accgtggact acacgcccgg agatggcaaa
    55081 cgggtgcccc agtaaacttt ctgccagagc cagattgtca tcagccagcg cctgacgcac
    55141 ggcggtgctg ctgatacgca caccaccttc gcaaaaagtt tgcgtactgg tgatatcgaa
    55201 gccgtattcc atgccagctt tctgtaataa caagaaatcg ccttcacgac cagcgccaaa
    55261 gcggaaatca tcacctacgg caagaaattt tacgcgcaaa tgcttcacca aaagatcgct
    55321 gatgaaattt tgcgcggtta acgccgcaaa acgcctgtcg aaacgcacgc acagcacgta
    55381 atcaacgcca cactctgcaa ggtaacgcag tttttcccgc agccgggtca gtcttgccgg
    55441 ggctttatcg gtagcaaaca gttccagtgg ttgaggttca aaaagcatca ccatcaccgg
    55501 taagttgcgc ttgcgccctt cttcctgcaa gccctgtaac agcgcgcgat gaccgcgatg
    55561 cacgccgtcg aaattaccaa tagtcagcac acacccttct tgcggggcct ggctgagatt
    55621 atgtatgccg cgtatcagct tcatgtctgg ctcaaaacag tgaaaatcgt ccgagtatac
    55681 cttgtacagc ggtcaaggtt aaccggcgat tgagtaccga aatcagaaag aagtggcttt
    55741 tcattgccat ggcgcaaatc acgggaagaa actgaccgcc tgctgcaatt tttatcgcgg
    55801 aaaagctgta ttcacacccc gcaagctggt agaatcctgc gccatcacta cgtaacgagt
    55861 gccggcacat taacggcgct tatttgcaca aatccattga caaaagaagg ctaaaagggc
    55921 atattcctcg gcctttgaat tgtccatata gaacacattt gggagttgga ccttggctaa
    55981 tatcaaatca gctaagaagc gcgccattca gtctgaaaag gctcgtaagc acaacgcaag
    56041 ccgtcgctct atgatgcgta ctttcatcaa gaaagtatac gcagctatcg aagctggcga
    56101 caaagctgct gcacagaaag catttaacga aatgcaaccg atcgtggacc gtcaggctgc
    56161 taaaggtctg atccacaaaa acaaagctgc acgtcataag gctaacctga ctgcacagat
    56221 caacaaactg gcttaatcgc caatttgctg aagctttgtg aaaaagcccg cgcaagcggg
    56281 tttttttatg cctgctgctt ttgtgatgcg tgagagaaca cactgccggt tgccagatag
    56341 tcaaaagaga catgccgttg tactatctga actcttcgtg gcttaagaat cgtagaacca
    56401 tcggctgata cattctgaac aagaatatct actcaatctc cagactcccc cacaaagaat
    56461 atggatattg tgatacacat tgaggtatta aacttaagac cactctattt ttgaattaaa
    56521 ttcctatata ctttaaccat tcataaatat ctcctcatta gac
//
LOCUS       DAJVQH010000012        54865 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJVQH010000012 DAJVQH010000000
VERSION     DAJVQH010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.012"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..354
                     /locus_tag="OQM87_000829"
     CDS             <1..354
                     /locus_tag="OQM87_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCS1507074.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OQM87_000830"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OQM87_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCS1507075.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OQM87_000831"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OQM87_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCS1507076.1"
                     /translation="MKHLHQFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3109..3261
                     /gene="mokC"
                     /locus_tag="OQM87_000832"
     CDS             3109..3261
                     /gene="mokC"
                     /locus_tag="OQM87_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCS1507077.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OQM87_000833"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OQM87_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCS1507078.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OQM87_000834"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OQM87_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCS1507079.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="OQM87_000835"
     CDS             6877..7281
                     /locus_tag="OQM87_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCS1507080.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="OQM87_000836"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="OQM87_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS1507081.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="OQM87_000837"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="OQM87_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCS1507082.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OQM87_000838"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OQM87_000838"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCS1507083.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OQM87_000839"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OQM87_000839"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1507084.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12152
                     /locus_tag="OQM87_000840"
                     /pseudo
     CDS             10704..12152
                     /locus_tag="OQM87_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12222..12998
                     /gene="yaaA"
                     /locus_tag="OQM87_000841"
     CDS             12222..12998
                     /gene="yaaA"
                     /locus_tag="OQM87_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCS1507085.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13151..13447)
                     /locus_tag="OQM87_000842"
     CDS             complement(13151..13447)
                     /locus_tag="OQM87_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1507086.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13661..14947)
                     /gene="thrC"
                     /locus_tag="OQM87_000843"
     CDS             complement(13661..14947)
                     /gene="thrC"
                     /locus_tag="OQM87_000843"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCS1507087.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14948..15880)
                     /gene="thrB"
                     /locus_tag="OQM87_000844"
     CDS             complement(14948..15880)
                     /gene="thrB"
                     /locus_tag="OQM87_000844"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCS1507088.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15882..18344)
                     /gene="thrA"
                     /locus_tag="OQM87_000845"
     CDS             complement(15882..18344)
                     /gene="thrA"
                     /locus_tag="OQM87_000845"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCS1507089.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18370..18489)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18425..18490)
                     /gene="thrL"
                     /locus_tag="OQM87_000846"
     CDS             complement(18425..18490)
                     /gene="thrL"
                     /locus_tag="OQM87_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCS1507090.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18704..19390)
                     /locus_tag="OQM87_000847"
     CDS             complement(18704..19390)
                     /locus_tag="OQM87_000847"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1507091.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19790..19930)
                     /locus_tag="OQM87_000848"
     CDS             complement(19790..19930)
                     /locus_tag="OQM87_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507092.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20026..20742
                     /gene="arcA"
                     /locus_tag="OQM87_000849"
     CDS             20026..20742
                     /gene="arcA"
                     /locus_tag="OQM87_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCS1507093.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20801..22153)
                     /gene="creD"
                     /locus_tag="OQM87_000850"
     CDS             complement(20801..22153)
                     /gene="creD"
                     /locus_tag="OQM87_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCS1507094.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22211..23635)
                     /gene="creC"
                     /locus_tag="OQM87_000851"
     CDS             complement(22211..23635)
                     /gene="creC"
                     /locus_tag="OQM87_000851"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCS1507095.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     VAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(23635..24324)
                     /gene="creB"
                     /locus_tag="OQM87_000852"
     CDS             complement(23635..24324)
                     /gene="creB"
                     /locus_tag="OQM87_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCS1507096.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSLGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24337..24810)
                     /gene="creA"
                     /locus_tag="OQM87_000853"
     CDS             complement(24337..24810)
                     /gene="creA"
                     /locus_tag="OQM87_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCS1507097.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25021..25890
                     /gene="robA"
                     /locus_tag="OQM87_000854"
     CDS             25021..25890
                     /gene="robA"
                     /locus_tag="OQM87_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCS1507098.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25887..26534)
                     /gene="gpmB"
                     /locus_tag="OQM87_000855"
     CDS             complement(25887..26534)
                     /gene="gpmB"
                     /locus_tag="OQM87_000855"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCS1507099.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26586..27107
                     /gene="yjjX"
                     /locus_tag="OQM87_000856"
     CDS             26586..27107
                     /gene="yjjX"
                     /locus_tag="OQM87_000856"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCS1507100.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(27192..27518)
                     /gene="trpR"
                     /locus_tag="OQM87_000857"
     CDS             complement(27192..27518)
                     /gene="trpR"
                     /locus_tag="OQM87_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCS1507101.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27608..29545)
                     /gene="sltY"
                     /locus_tag="OQM87_000858"
     CDS             complement(27608..29545)
                     /gene="sltY"
                     /locus_tag="OQM87_000858"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCS1507102.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     EIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29756..31423
                     /gene="ettA"
                     /locus_tag="OQM87_000859"
     CDS             29756..31423
                     /gene="ettA"
                     /locus_tag="OQM87_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCS1507103.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31530..31697)
                     /locus_tag="OQM87_000860"
     CDS             complement(31530..31697)
                     /locus_tag="OQM87_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507104.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31731..32963)
                     /gene="nadR"
                     /locus_tag="OQM87_000861"
     CDS             complement(31731..32963)
                     /gene="nadR"
                     /locus_tag="OQM87_000861"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCS1507105.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32984..34366)
                     /gene="radA"
                     /locus_tag="OQM87_000862"
     CDS             complement(32984..34366)
                     /gene="radA"
                     /locus_tag="OQM87_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCS1507106.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34415..35383)
                     /gene="serB"
                     /locus_tag="OQM87_000863"
     CDS             complement(34415..35383)
                     /gene="serB"
                     /locus_tag="OQM87_000863"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCS1507107.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35489..36133
                     /locus_tag="OQM87_000864"
     CDS             35489..36133
                     /locus_tag="OQM87_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCS1507108.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36161..37177
                     /gene="lplA"
                     /locus_tag="OQM87_000865"
                     /pseudo
     CDS             36161..37177
                     /gene="lplA"
                     /locus_tag="OQM87_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37209..37472
                     /locus_tag="OQM87_000866"
     CDS             37209..37472
                     /locus_tag="OQM87_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1507109.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37633..38352)
                     /gene="deoD"
                     /locus_tag="OQM87_000867"
     CDS             complement(37633..38352)
                     /gene="deoD"
                     /locus_tag="OQM87_000867"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCS1507110.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38409..39632)
                     /gene="deoB"
                     /locus_tag="OQM87_000868"
     CDS             complement(38409..39632)
                     /gene="deoB"
                     /locus_tag="OQM87_000868"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1507111.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39684..41006)
                     /gene="deoA"
                     /locus_tag="OQM87_000869"
     CDS             complement(39684..41006)
                     /gene="deoA"
                     /locus_tag="OQM87_000869"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCS1507112.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLDGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41133..41912)
                     /gene="deoC"
                     /locus_tag="OQM87_000870"
     CDS             complement(41133..41912)
                     /gene="deoC"
                     /locus_tag="OQM87_000870"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCS1507113.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42170..43711
                     /locus_tag="OQM87_000871"
     CDS             42170..43711
                     /locus_tag="OQM87_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCS1507114.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDTRCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43683..44546
                     /locus_tag="OQM87_000872"
     CDS             43683..44546
                     /locus_tag="OQM87_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCS1507115.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44760..45542)
                     /locus_tag="OQM87_000873"
     CDS             complement(44760..45542)
                     /locus_tag="OQM87_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1507116.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45539..46612)
                     /locus_tag="OQM87_000874"
     CDS             complement(45539..46612)
                     /locus_tag="OQM87_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCS1507117.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46734..46895)
                     /locus_tag="OQM87_000875"
     CDS             complement(46734..46895)
                     /locus_tag="OQM87_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCS1507118.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(47022..47627)
                     /gene="osmY"
                     /locus_tag="OQM87_000876"
     CDS             complement(47022..47627)
                     /gene="osmY"
                     /locus_tag="OQM87_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCS1507119.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(48020..49609)
                     /gene="prfC"
                     /locus_tag="OQM87_000877"
     CDS             complement(48020..49609)
                     /gene="prfC"
                     /locus_tag="OQM87_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCS1507120.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49700..50377)
                     /gene="yjjG"
                     /locus_tag="OQM87_000878"
     CDS             complement(49700..50377)
                     /gene="yjjG"
                     /locus_tag="OQM87_000878"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCS1507121.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50392..50838)
                     /gene="rimI"
                     /locus_tag="OQM87_000879"
     CDS             complement(50392..50838)
                     /gene="rimI"
                     /locus_tag="OQM87_000879"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1507122.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50807..51220)
                     /gene="holD"
                     /locus_tag="OQM87_000880"
     CDS             complement(50807..51220)
                     /gene="holD"
                     /locus_tag="OQM87_000880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCS1507123.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51323..52354
                     /gene="rsmC"
                     /locus_tag="OQM87_000881"
     CDS             51323..52354
                     /gene="rsmC"
                     /locus_tag="OQM87_000881"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCS1507124.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVITQTGRFKVYRA
                     IMTRQAKKG"
     gene            52631..52717
                     /locus_tag="OQM87_000882"
     tRNA            52631..52717
                     /locus_tag="OQM87_000882"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52665..52667,aa:Leu,seq:cag)
     gene            52746..52832
                     /locus_tag="OQM87_000883"
     tRNA            52746..52832
                     /locus_tag="OQM87_000883"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52780..52782,aa:Leu,seq:cag)
     gene            52867..52953
                     /locus_tag="OQM87_000884"
     tRNA            52867..52953
                     /locus_tag="OQM87_000884"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52901..52903,aa:Leu,seq:cag)
     gene            complement(52992..53228)
                     /locus_tag="OQM87_000885"
     CDS             complement(52992..53228)
                     /locus_tag="OQM87_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCS1507125.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            53369..54157
                     /gene="fhuF"
                     /locus_tag="OQM87_000886"
     CDS             53369..54157
                     /gene="fhuF"
                     /locus_tag="OQM87_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCS1507126.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(54195..>54865)
                     /gene="bglJ"
                     /locus_tag="OQM87_000887"
     CDS             complement(54195..>54865)
                     /gene="bglJ"
                     /locus_tag="OQM87_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCS1507127.1"
                     /translation="SRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQESF
                     QDAMARISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLIGSL
                     SPSPLDGVLSKASTLEIFHQEFFLSLNGVRQATDRLNNQWYINQSRTLSPTEREILRF
                     MSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEASNV
                     LHPY"
BASE COUNT        13116 a        14825 c        13710 g        13214 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaaatc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagaaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagagaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cgctaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aaggcagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcgt catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaattgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcta ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggtg
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcacc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ttctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataagg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggagctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagccgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcattcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagcggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagcg tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggagcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcgccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc acgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacagt ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc ggtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccgccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgcgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tattgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 cgggaatggg atcgacgcca aacgcggcag cggcagcggc agcggcagcg gcagcggcat
    11581 cctggcctcc gcatccggca gcacaaggga ttgtccagat gattggcatt tttatcgaca
    11641 ccctggtcat ctgtacggca agcgccatgc tgatattact ggcgggtaac ggcacaacct
    11701 acatgccgct ggaaggtatt cagcttatcc agaaggcgat gcgggtgcta atgggttcct
    11761 agggtgctga atttgttacc ctcgtggtta ttctgtttgc cttcagctcc atcgttgcca
    11821 actacattta tgccgaaaac aatctcttct ttttacgcct gaacaaccct aaagcgatct
    11881 ggtgtttgcg gatctgcacc ttcgcaacgg tcatcggcgg caccttgcta agccttccgc
    11941 tgatgtggca actggcagat atcataatgg cctgcatggc tattaccaat ttgaccgcca
    12001 ttttactgct ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac
    12061 tcggcgtgcg cccggtgttt gatccgttgc gttatccgga tatcggtcgc cagctttctc
    12121 cggacgcgtg ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt
    12181 tttttgctaa agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac
    12241 ctgcgaaaac gcttgattac caaagcccgt tgaccaccac gcgctatacg ctgccggagc
    12301 tgttagacaa ttcccagcag ttgatccatg aagcgcggaa actgacgcct ccgcagatta
    12361 gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc tttcatgact
    12421 ggcagccaga tttcacgccg gcgaatgccc gccaggcgat tctggcgttt aaaggtgatg
    12481 tctacaccgg cttgcaggcc gaaaccttca gcgaagacga tttcgatttt gcccaacagc
    12541 atttgcgaat gctttccggc ttgtatggcg tactgcgccc gctcgattta atgcagcctt
    12601 atcgtctgga aatggggatc cgtcttgaga atgcccgagg gaaagatctg tatcaattct
    12661 ggggagatat catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtgg
    12721 tgattaatct ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg
    12781 agattatcaa gccggtgttc ctcgatgaga agaacggcaa atttaagatc atcagcttct
    12841 acgctaagaa agcacgcggt ctgatgagtc gtttcattat tgaaaatcgg ctgaccaaac
    12901 cagagcaact gactggtttt aatagcgaag gttacttctt tgatgaagat tcctccagca
    12961 atggcgaact ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc
    13021 ggcgtgaacg ccttatccgg cctacaaaag catgcagatt caatatattg cagaattaac
    13081 gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg aatgccggat tgttacccgg
    13141 catttgtcat ttagcggtga tgtttgcctg gaccatgacc gccgtgatga tcatgaggag
    13201 ctttcttatg gtggcgcggc ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc
    13261 attcataatg ttgtttccac cagccgtggt cgcgccagtg acctccatcc cagtaatagc
    13321 cacgattatc acgatcgcct atctgtaatt ttactgacgg gactaacgta atttccgcag
    13381 cctgtgctgc catgggagcg accagaacca gggaaagtgc gagtacgata gattgcatct
    13441 ttttcacagg ttactccttt tatcattgcc cgttatgggt cgatatccgg agattacgcg
    13501 atgagaacga cggttgttat tctccgcaat cctaatctct aagttaccgc atttattgat
    13561 aatttctcct ttttccctgt cgtttttctc cataatttct tcataaaaaa gccgggctgc
    13621 ataaaagcaa acccggcctg attgagataa tgaatagatt ttactgatga ttcatcatca
    13681 atttacgcaa cgcagaaaaa tcggcgggca gattatgtga aagcaagggt aaatcagcac
    13741 gttctgccag ctcttttggc agatccaacg tttcaccgag aatcgcttcc acgctctctt
    13801 taaatttcgc cggatgcgcg gtgccgagga acaagccata ttcgcctgga ttcaactggt
    13861 cacgcagcgc acgatacgct acggcagcgt gcggctccga ggtgtagccc agttctttta
    13921 actcacgcat tgtctgttgc gtggtttcat catccacggc tgcataaccc agctctttca
    13981 gttgccagat tttgcggcgg aacaactctt ccacacgcgg ccagttgttc ggctgactca
    14041 cgtccatcgc gttggataac gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca
    14101 ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc accggcagac
    14161 cgaatgactt cgccagcaga cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga
    14221 caaccagctg gttgcgcgcc tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa
    14281 tctgcgccag caaacggctg atgttaatcg agttagccga gtttaacccc agcgccactt
    14341 tcagttcttc atcatcaaac gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt
    14401 cgatggcaac cgtttcgata ttgccgccca acgtacagaa cagtttttct tgcagtggac
    14461 tgattttgcc tcgtggatag aggataacca ctttcacatt cggcaaaccg tagaaagcat
    14521 gagccactgc agctccggta tcaccggagg tcgcggtcag aatggtcact ggcttatcat
    14581 ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta aatgccagcg
    14641 ttggcccgtg gaacaattcc agacaaccga catcgctttc aacattggcg accggagccg
    14701 ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc ctgtgggatt tcatcaccaa
    14761 taaacgccga gaggatcttc gcactgcggg tgacaaaatc cagcttcagc atctcatcaa
    14821 tttcagtcag gctgaattcc ggcaggtcgt gcggaaaaaa cagcccctga tttttgccca
    14881 acccctgggt tacggcttgc gcaaagctga cctgctcgtt gtgatctttc agattgtaga
    14941 gtttcattta gttttccagt actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa
    15001 aaccttcctg attttgcagg tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat
    15061 ccggcttgtc acatagagcg aacaaggtcg ggccggagcc ggagataccg ctcgctaccg
    15121 cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc aggcagtaac cgttcacggt
    15181 agggttcagc gataacatct ttcatcagct tcgcggcaag ctcaggctga cgggaatagc
    15241 aggcgtgaat gaagccagcc agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact
    15301 gcgccggtaa aatagcccgg gcttctgccg tcgagacttt aattcccgga tacgccagca
    15361 cccacagcca ctcatcaaac cctggcactt gctggctgat gatgtcgttt tcttcgatca
    15421 tcaactgcat accaccaaga aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga
    15481 tacgtccttc cagctcgccc atcaaagcca gcaaacgagt gtcattaagc ggcttgccgc
    15541 agtgttcatt catcgccatc agcgccgcga ccaccgaaca ggcgctggag cctaagcccg
    15601 aaccgatcgg catattcttt tccagagtca tcgccactgg aatttgcttg ccaagctcct
    15661 ggcaaaaacg ctcccagcac tgataaacga tattttcccg tggttctgac ggcagcttat
    15721 cggcaaagcg tccgaggttg ttgagactga atgtctctgc cgcctcaacc gtgactacat
    15781 ctccgagcaa tgcaccatca acgggtgcca ccgccgcccc gagcacatca aacccgacgc
    15841 tcatattggc actggaagcc ggggcataaa ctttaaccat gtcagactcc taacttccat
    15901 gagagggtac gtagcagatc ggcaaagaca ccggcagcgg taacgtcatt gcccgcacca
    15961 tatccgcgca gcaccaacgg cagcggctga taatagtggc tataaaaggc cagggcgttt
    16021 tcgccatttt tcactttgaa cagcggatca ttcccatcca cttcggcaat cttcacgcgg
    16081 cagacaccat cttcatcaat attgccaaca tagcgcaaaa cttttccttc atcacgggcc
    16141 ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat aaaagcggca
    16201 acatcaccct cggcgttaaa ctctgcgggc agcacaggtt caatttcaat atccgccagc
    16261 tccagttcac gtcccgtttc acgagcgaga atcaatagtt tacgcgccac atccatacca
    16321 gaaagatcat ctcgcgggtc cggttcggta taacccattt cccgcgccag cgtggtcgcc
    16381 tcggagaaac tcatgccttc gtctaacttg ccgaagatat aagaaagcga acctgaaaga
    16441 atgccggaga acttcaccaa ttcatcacct gcattgagca gattttgcag gttctcaata
    16501 accggtaatc cagccccaac gttggtgtca tagaggaatt tacgccgcga tttttccgcc
    16561 gcataacgca actgatgata gtaatccatc gacgaggtgt tggccttttt gttcggcgtg
    16621 acaacgtgga aaccttcgcg caggaagtcg gcatattgat ccgccactgc ctggctggag
    16681 gtgcagtcaa caatgaccgg gttcagcaga tgatattctt tcacgaggcg aattaagcgc
    16741 ccgagattaa acggctcttt ggcttgtgca agttcttcct gccagttttc cagattaagg
    16801 ccatgcacac tagtgagcag agccttcgag ttggcaacgc cgcagacacg taagtcgata
    16861 tgtttattct tcagccagct ttgctgacgc ttcagttgct ccagcagcgc accgccaacg
    16921 ccaccgacgc caatcacaaa cacttcgata acctgatcgg tattgaacag catctgatga
    16981 gtaacgcgca cgccagtggt cgcatcatcg ttatttacca cgacagagat tgagcgttca
    17041 gaagatccct gagcaatggc gacaatgttg atattggcgc gggccagcgc ggcaaagaat
    17101 ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat aatggccagc
    17161 cgttccgtca ccgccagcgg ctccagtaag ccttctttca gttccaggta gaactcttcc
    17221 tgcattgccc gttcagctcg cacacagtcg ctttgtggaa cgcagaaact gatactgtat
    17281 tcggaagatg attgcgtaat cagcaccacg gaaatacggg cgcgtgacat tgcagcaaag
    17341 acgcgcgccg ccatgccaac catccctttc atccccgggc cggaaacgct gaacattgcc
    17401 atgttattca gattggaaat gcccttgacc ggtaattcgt cttcatcacg gctggcacca
    17461 atgagcgtac ctggtgcttg aggatttccg gtatttttaa tcaggcaagg gatctggaac
    17521 tgggcgatgg gggtaatggt gcgggggtga agaactttag cgccgaagta ggaaagctcc
    17581 atcgcttcct ggtaggacat cgacttcaac aacctcgcat cgggcacctg acgcgggtcg
    17641 caggtataga ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg taaacaggca
    17701 gccagcaccg ccgcggagta gtcggaaccg ttacgtccaa gcaccaccag ttcgcctttt
    17761 tcattaccgg cggtgaaacc tgccatcaac accatgtgat cagccggaat gcggcttgcc
    17821 gcaatacggc gggtggactc agcaatatcg acggtagatt cgaggtaatg ccccactgcc
    17881 agcagttttt cgaccggatc gataacggta acgttgtgac cacgcgcttc taacacgccg
    17941 gccataatgg cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc
    18001 gggcactgcc ccaacaaact aatgccatgc aggacatgtt ttatttgggc aaattcctga
    18061 tcgacgaaag ttttcaattg cgccagcggg aaccccggct gggcggcggc gagtcccgtc
    18121 aaaagttcgg caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta
    18181 atggtttttt caatcatcgc caccaggtgg ttggtgattt tggcgggggc agagaggacg
    18241 gtggccacct gcccctgcct ggcattgctt tccagaatat cggcaacacg cagaaaacgt
    18301 tctgcatttg ccactgatgt accgccgaac ttcaacactc gcatggttgt tacctcgtta
    18361 cctttggtcg aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta
    18421 cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa
    18481 tgcgtttcat ggatgttgtg tactctgtaa tttttatctg tctgtgcgct atgcctatat
    18541 tggttaaagt atttagtgac ctaagtcaat aaaattttaa tttactcacg gcaggtaacc
    18601 agttcagaag ctgctatcag acactctttt tttaatccac acagagacat attgcccgtt
    18661 gcagtcagaa tgaaaagctg aaaaatactt actaaggcgt tttttatttg gtgatatttt
    18721 tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca
    18781 ggcgttgctg taaccagtcg accagtttta tgtcatctgc cactgccaga gtcgtcagca
    18841 atgccatgac tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt
    18901 tcgccggttg ttgtattaat gttgctaatt gatagcaata gaccatcacc gcctgcccca
    18961 gattgagcga aggataatcc gccaccatcg gcacaccagt aagcacgtca gccaacgcta
    19021 actcttcgtt agtcagcccg gaatcttcgc gaccaaacac cagcgcggca tggctcatcc
    19081 atgaagattt ttcctctaac agcggcacca gttctactgg cgtggcgtag taatgatatt
    19141 tcgcccgact acgcgcagtg gtggcgacag tgaaatcgac atcgtgtaac gattcagcca
    19201 gtgtcgggaa aactttaata ttatcaataa tatcactaga tccatgtgcg acccagcggg
    19261 tggctggctc caggtgtgcc tgactatcga caatccgcag ttcgctaaaa cccatcgttt
    19321 tcattgcccg cgccgctgcc ccaatatttt ctgctctggc gggtgcgacc aggataatcg
    19381 ttatacgcat attgccactc ttcttgacca aataaccgcg aacagggtga tcactgtcaa
    19441 cttattacgc ggtgcgaatt tacaaattct taacgtaagc cgcagaaaaa gccctttact
    19501 tagcttaaaa aaggctaaac tattttctga ctgcactaac ggtttaattg ttaaaaaatg
    19561 ctacatatcc ttctgtttac ttaggataat tttataaaaa ataaatctcg acaattggat
    19621 tcaccacgtt tattagttgt atgatgcaac tagttggatt attaaaataa tgtgacgaaa
    19681 gctagcattt agatacgatg atttcatcaa actgttaacg tgctacaatt gaacttgata
    19741 tatgtcaacg aagcgtagtt ttattgggtg tccggcccct cttagcctgt tatgttgctg
    19801 ttaaaatggt taggatgaca gccgtttttg acactgtcgg gtcctgaggg aaagtaccca
    19861 cgaccaagct aatgatgttg ttgacgttga tggaaagtgc atcaagaacg caattacgta
    19921 ctttagtcat gttacgccga tcatgttaat ttgcagcatg catcaggcag gtcagggact
    19981 tttgtacttc ctgtttcgat ttagttggca atttaggtag caaacatgca gaccccgcac
    20041 attcttatcg ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg
    20101 gaaggctatg atgttttcga agcgacagat ggcgcggaaa tgcatcagat cctctctgaa
    20161 tatgacatca acctggtgat catggatatc aatctgccgg gtaagaacgg tcttctgtta
    20221 gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac
    20281 gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg actacatcac caaaccgttc
    20341 aacccgcgtg aactgacgat tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt
    20401 actgtcagcg aagaacgtcg tagcgttgaa agctacaagt tcaatggttg ggaactggac
    20461 atcaacagcc gttcgttgat cggccctgat ggcgagcagt acaagctgcc gcgcagcgag
    20521 ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg
    20581 ctgaagaaaa tgaccggccg tgagctgaaa ccgcacgacc gtactgtaga cgtgacgatc
    20641 cgccgtattc gtaaacattt cgaatctacg ccggatacgc cggaaatcat cgccaccatt
    20701 cacggtgaag gttatcgctt ctgcggtgat ctggaagatt aatcggcttt accaccgtca
    20761 aaaaaacggc gctttttagc gccgttttta tttttcaacc ttatttccag atacgtaatt
    20821 catcgtccgt tgtaacttct ttactggctt tcattttcgg cagtgaaaac gcataccagt
    20881 cgatattacg agtcacaaac atcatgccgg ccagcgccac caccagcaca ctggttccca
    20941 acaacagcgc gctatcggca gagttgagca gtccccacat cactccatcc agcaacaaca
    21001 gcgcgagggt aaacaacatg ctattgcgcc agcctttcaa taccgcctgc aaataaatac
    21061 cgttcattaa cgccccaatc agactggcga ttatccatgc cacggtaaaa ccgatatgtt
    21121 cagaaagcgc cagcaagagc aaataaaaca tcaccaatga aagccccacc agcaaatatt
    21181 gcattgggtg taaacgttgc gcggtgagcg tttcaaaaac aaagaacgcc ataaaagtca
    21241 gtgcaatcag cagaatggcg tacttagtcg cccggtccgt taattggtat tgatcggctg
    21301 gcgtcgtcac tgcgacgcta aacgcgggga agttttccca gccggtatca ttgcctgaag
    21361 caaaacgctc accgagatta ttagcaaacc agctgctttg ccattgcgcc tgaaaacctg
    21421 actcgctagc ttcccgtttg gctggtagaa aatcacctaa aaaactggga tgcggccagt
    21481 tgctggttaa ggtcatttcg ctattacgcc cggcaggcac cacagaaaga tcgccggtac
    21541 cgcttaaatt cagtgccata ttcagctgca ggttctgctt ccgccagtcc ccttcgggta
    21601 aagggatatg cacgccctgc ccgccttgct ctaacccggt accgggttca atggtcagtg
    21661 ccgttccgtt aacttcaggc gctttcacca caccaatacc acgcgcatcc ccgacgctaa
    21721 tcacaataaa gggcttgcct aagatgatat ttggcgcgtt gagctcgcta agacgcgaaa
    21781 catcgaaatc ggcttttagc gttaaatcac tgtgccagac ctgaccggta taaatcccta
    21841 tcttgcgttc ttccacgttc tgattgccat caaccatcaa tgactcaggt aaccaaaaat
    21901 ggataaaact tcgtttccgc tccacggttt tatcctcttc ctgcaccgta taaagctcgg
    21961 tcacaggaat agcgataagc ggcccaacga gtttttgcgg cccgctggta ctttggcgaa
    22021 tcgcatcttc cacatcgcta cggtaatcgg cacgttcgac aatcacttgc cgaatcagca
    22081 ttatcggaat caacaacagc aatactgcac caaacaaggt ggtcattttc cagaacaggg
    22141 gggatttcaa catagtcttc tcctttgcaa tggcagcagg atacgaaggc tatgtgggaa
    22201 gaatttgaag ctatgtgaag ttacggtgaa gtcgtagcga ggccagcacg ccaccttcct
    22261 gcacgttgcg cagcgtgact tcgccgttaa acaaacgggc gacctcactg acgaacgcca
    22321 accccagacc gctgcttttt tgcccatttg cacgcggcaa agagtaaaag cgttcaaaaa
    22381 tacgtgacag cgcgtagtca ggaataccac taccggtatc cagcacctta agcgtgacgt
    22441 gttcctgatc cacttcggcg cttagcgtta tgcaaccgct ctcgggagta aaatcgatgg
    22501 tgttatccag taaattcccc agcgcctgat ccagtaacgc cggttcagca gcaacattaa
    22561 cctcggtggg cataacatgc aaagtgatgt ttttttctgc caactgcacg gtgcgcgctt
    22621 cgctgacgcg gcgaaataat gccgccacat caacagcagt cagaacgact tcctgacgat
    22681 tctccagtct tgcctggcgt agtaacgttt ccaccagcgc ctgcattcgc gcattttgtg
    22741 tcagaatgtt gtcagtaaaa cgagccacta cttcaggcgg cggaccttcg cgtaaaattt
    22801 ccgccacgcc acgaatcgcc gccagtgggc tttttagctc atgggttaac gcataaacat
    22861 actgctcaat atagtttttc ccttccagct tcacgcgcat actttccagc gcctgcgcga
    22921 gtttacgcag ctcgctacta ccgagatcgg gaagaggaac gggcttattg tcagtgacgg
    22981 aatcagcata gcgagtgagc cgggcaatgg agcggttgat ccaccaaacc atgcctgcgc
    23041 caatcaccag tgcaatcccc aacaaaatgg cgctggccca taaaattcgc cgctcgctgc
    23101 gtttaatgac cggagccatc gccgcgttcg gtttgcctac gctcaaaacg ccaataagcc
    23161 gcgagccgcc cataatcggc gcggcaacat acatcaccga actttcggga tcggcaggat
    23221 tttgcaacgt gctgcgcgca ccatactgac cacgcaacgt tagccagacg tcattccagc
    23281 gcgaataatc ctgtccaacg gctttatttg ccgaatcgaa caatactttg ccatgcgcat
    23341 cggtcatata gacatgatat tcattgcgca ctttgttaat gccaccaata ttggcgcgaa
    23401 acgggcgatg ttgtagctga ttaaacgcct gcgccagttg cccatgcgtt gggtccccag
    23461 agagcaaatc gggacgcgcc agctccgcaa gtaacgttgc cgtgtcgatc aacgtcccct
    23521 ccgttgctct tcgcacgccc ggtttaactt ctttgacaaa aatagccagt acgaaccagg
    23581 ccgccaccgc caccagtaaa aaatagccca gcagcaaccg catgccgata cgcattacaa
    23641 gcccctcagg ctatatccca tgccgcgatg agtattaatc ggtgaaagat cggggttgat
    23701 ggcgcgcagc ttggcacgca gcgttttaat gtggatatcg acggtgcgat cgtaggtgtc
    23761 ctgcgcatct tcccacacgc tatccattag ttgctggcgg gaccagacgc ggcctagcga
    23821 cttgagtaac gtcttcagca ataaaaactc ataccgcgtc agcgttaatg gcgtgtcaaa
    23881 ccagctgatc tgcgccgcgg gttcattcaa ttcaaaatga ccaatacgga tgacgggaga
    23941 cggcgtcgag aacttcttca cccgacgcag taaggtgcgc accctggcgc acacttcgcg
    24001 gggtgaaaac ggtttagcca catagtcgtc agcgccaatt tccagcccaa gcaggcgatc
    24061 gacctcttca cttcgggccg tcaggaacag tacaggtaac gccggatgga gcgccagtaa
    24121 ctggcggcac aattcaaagc cactgatatc aggcaaacca acgtcgagaa tcatgacgtc
    24181 gggaacctgc tggcgagctt tatccagcac cggcaaacct cgctcaaaga cctcgacggc
    24241 aaaaccttcc tgctgcaaca tgtagaccag cgtgtcggct atcccttgct catcttccac
    24301 taaccagacc gtttcccgtt gcataaatcg cctctgttat tgccgccacg gcatgacagg
    24361 aaccgcgcta atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt aagccagata
    24421 agcgagcgcg ttgcgtttgg cgtcataaaa gcgcacgacc tgtaacgact taaagaccag
    24481 ggacgtgcgt tttttgaata ctacctcgcc ctgagctttg ccgtttttaa tacgatccga
    24541 cagttcaatc ggcccgactt gctgacaaga aatggccgca tcggaggtat cttctgccag
    24601 gcccaatccc cctttaatac caccggtttt cgcccggctc acataacagg tgacattttt
    24661 cacatcggga tcgtcaaagg cttccacaac aattttgtga tccgggccga tcattttaaa
    24721 tacggtgtcg accgaaccaa tctcttctgc atgagccaat ggccccagca ttatcaggct
    24781 taaagacagg atcaaatgct tgtatttcat attgttacca tttttatcta ttacacgagc
    24841 aattagttcg tcacggtaaa taacatattg attaagaaca aaatctcaat tcttttattt
    24901 ccgtcaggcc ctaaaacata ctctactaag aaaaaaacac tgaatgctaa aacagcaaaa
    24961 aatgctatta tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg aggatatttt
    25021 atggatcagg ccggcattat tcgcgacctt ttaatctggc tggaaggtca tctggatcag
    25081 cccctgtcgc tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca cttacagaga
    25141 atgtttaaag atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg tcgtttgtcg
    25201 aaatcggcgg tcgcactacg cctgactgcg cgtccgattc tggacatcgc gctgcaatac
    25261 cgcttcgact ctcaacagac atttacccgc gcattcaaga agcagtttgc ccagactcct
    25321 gcactttacc gccgttctcc tgaatggagc gcctttggta ttcgcccgcc gctacgtctg
    25381 ggtaaattca caatgccaga gcacaaattt gtcaccctgg aagatacgcc gctgattggt
    25441 gttacccaga gctactcctg ttcgctggag caaatctctg atttccgcca tgaaatgcgt
    25501 tatcagttct ggcacgattt tctcggcaac gcgccgacca ttccgccggt gctctacggc
    25561 ctgaatgaaa cgcgtccgag tcaggataaa gacgacgaac aagaggtatt ctataccacc
    25621 gcgttagccc aggatcaggc agatggctat gtactgacgg ggcatccggt gatgctgcag
    25681 ggcggcgaat atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca ggagtttatc
    25741 ctgacggtat acggaacgtg catgccaatg ctcaatctga cgcgccgtaa aggtcaggat
    25801 attgagcgat actacccggc agaagacgcc aaagcgggag atcgcccaat taatctgcgc
    25861 tgtgaactgc tgattcctat ccgccgttaa cgctgcagct catctaatgc aggggcgtcc
    25921 agatgcgaga tgtcccctgc ggtttcaacg acccagccag acgccagcca caggctttcc
    25981 tgataatcca cgcgcgaaat agaacagtta cgcagacgta agcggcgctc tgcccatgct
    26041 ggtaatccga gaatcgtact caccagacat cccagtgcaa taccatggct caccagcagc
    26101 ggtcggcttc cctgcggtaa gtcccggcaa gattccagtg ccgcattgac gcgatcgctg
    26161 agctcctgca ttgactcgcc ttcaggaata cgcccgtcaa cggtgccatt gaccagctgc
    26221 cgacgccagt tctcttcttc ttcggtcaga gaatcgatat gtcttttttc cagcacaccc
    26281 atgtttaatt cacgcaggcg agaatcaaag atgatgtcac agccgcaggc ctgggcgatg
    26341 atttccgccg tacgccgggt gcgtcctaaa tcgctactga taatatgcgt aatgccaagc
    26401 tctttggcac gggttgccac ctgcatcgcc tgttgctcac ctttggcggt cagcgggctg
    26461 tcagactggc cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg gcggactagg
    26521 tatacctgta acatgctttt tttccgtata ctgctgtaac gttaattttc tgagtaatgc
    26581 tgattatgca ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag gccattctgc
    26641 aggcatttca cgagatcttc ggcgaaggat cctgccatat tgcatccgtt gccgtcgaga
    26701 gcggtgtacc ggaacaaccc tttggcagtg aggaaacgcg cgctggcgca cgaaatcggg
    26761 tagcaaatgc ccgccgttta cttccagagg ctgatttttg ggtggcgatt gaagctggca
    26821 ttgatggcga cagcactttc agttgggtgg tgattgaaaa cgccagccag cgcggcgagg
    26881 cgcgttcggc aactttgccg ttacctgcgg tgattctgga aaaagttcgt gaaggcgagg
    26941 cgcttggtcc cgtgatgtcg cgttataccg gtattgatga gattggccgt aaggaagggg
    27001 cgatcggcgt gtttaccgcc gggaaactca ctcgcgccag cgtctatcac caggcggtga
    27061 tcctggcgct gagtccgttt cacaatgccg tgtatcaggc attgtaggac ggataaggcg
    27121 ttcacgccgc atccgacatt cggtgcacga tgcctgatgc gacgctgccg cgtcttatca
    27181 ggcctacaaa atcaatcgct tttcagcaac acctcttcca gccactgacg cagctccacg
    27241 ggcgcggctt tcaggctgtt agatccacgc gtaatcgtcg cgatgcccgc gccgagttca
    27301 ttttttaact cacgctgact catttcgcca cgcaacagct cttcgacaat acgcacgcga
    27361 gtccccaacg cttcgcgctc atctggcgtc agcatcaggt ttaacaacgg taaatggaga
    27421 tcgttttggt aggcattctt aagcaggtcg acaaaacgta accactcctg gtgacgctgt
    27481 tctgccatcg ctgctgaata gggtgattgt tgggccataa tatgtcgcca ttgttagcgg
    27541 gggaagcaaa atgactcccc cggttgtact cgctaaagag tacgatagca tatcataaac
    27601 gtgcggatca gtaacgacgt ccccattccg tggcgctcat caacgtcggt ttatccccca
    27661 tgaaatagcg gtagtaagca tcataagcca gcacgttctt cacataaccg cgcgtctcgg
    27721 agaacggaat actctcgaca aatgccacag cgtcgatacg cccggcgctg ttgccaagcc
    27781 aggttcgcac ccgtccaggt ccggcgttat aagctgctga ggagaaaata cggttattgc
    27841 caaactgctg ataaacatat tgcaggtaac tggtgccaat gttgatgttc gtttccggat
    27901 ccagcaattg cccagggctg ctataaccag gaatagagaa catcttcacc gtatgggtcg
    27961 ctgtaccagg cataatctgc atcaggccgc tggcccctac cggtgatttc actttcggat
    28021 tccaggcgct ctcctgacgg gcaatcgcca tcgcatagct ttgcgggatc tctttcccac
    28081 tggtgtaacg tttgaaaaga tcgttgtaag ccagcgggaa tcgctcttcc agatgatccc
    28141 aaagcttccc ggcgatcgtt gcctgaacgc taagatccca ccattggtta ttgaaagcat
    28201 accgagccag ttgagcctgc tctgtttttg acttgctctt caccagattg gcccactcgc
    28261 tacgcgcggt attatcgaga ttccagtaca tcaactcgcg cacgcgcgcc atctccggcc
    28321 cctgagtcag ggtgctgtca acattctgcg gcgctttatc aatcttcagc tcatactctt
    28381 caccgatgcg ttgtgctgca accatcgggt agaaaccacg ctgttgcatg agttgatgca
    28441 aaatctcttt cgcttcagct tcacgtccgc gttccagcaa taaatccgcc tgccagtaac
    28501 gccattcatc tttctctttt gcttccatcg gcagacgcgc cagccaggta ttcaggccgc
    28561 gacgatcgcc ggtgccaagc gccattcgca cacggcgttc aatcaacgac gtggattgcg
    28621 agcgcataat ggcgtcatcg cgccatttcg cctgctcatc ggtgacatca ttgcccatca
    28681 aacgccaggc gacgatatcg cgcagctcct gaatctcatc ttcattaagc tgctgcgcct
    28741 gggcaagcga cgggatcatc agccgtgcat tttcggcatc ctgccgcgcc acactggcaa
    28801 acgccaccgc cgccatttga cgggtaaaat cggtcgcgcc ggttgtacgc gcgaaggtca
    28861 gtaccgtatt agggttgttc gccagtgaaa tgattgccga ggcgatagtc tggtaatcgg
    28921 caggcatctg ccctgccagc actgttacca ggcctgtgtt acccgctttc atcgccagac
    28981 ggatacgctc taaatacgcc agcggatctt gtttacctga cgcacgccag acgctaaata
    29041 acttgtcaca ggcgttaggc tggctcttgc cggttagcca cagctctttc gccccttgcc
    29101 aggcttcttc actctgcccg gtgttccatt tcgcatagta gtaattacat tgcgcttcgg
    29161 tagttccggg cttttccggg ctaaaggcta acaagccacg ccagtcttca cgccgcgcca
    29221 gttcattgac gaaacgagat tgcagcgtgc gagcgggagg aagcgtgggg ttagcgcgaa
    29281 caaagttagt gaccgtcacc gccggttgat tcatcaggtc atcggtgatc tggcggtatt
    29341 ccaggtaggg ataaagcgga taatccttca gtccaggcat catttgttcg accacatcca
    29401 tctgtcgatt atcccaggcc tgcttgattt gcgcgtaacg gctacgctgc tcatccagtg
    29461 agtcggctcg cgccacgctg ctgaccgtca gcagacagac accggcagcc aacagccgcc
    29521 aggtaacttg tttggctttt tccacaagcg catcctctaa gtgtaaatac atcaatgcag
    29581 cgtcataccg cgtaatagat ttatgctaac cagtcattgc cgtttacgcc acgttacgga
    29641 cactttttta cttttactgc gaaggtgatc gaccaactgg gattaggcgg ggaaacaggc
    29701 tacacttcgc ctttgaaaac tcatcatcac gataaacata gaggcgaagt ccaacgtggc
    29761 tcaattcgtt tataccatgc atcgtgtcgg caaagttgtt ccgccgaaac gtcatatttt
    29821 gaaaaacatc tctctgagtt tcttccctgg ggcaaaaatt ggtgtcctgg gtctgaacgg
    29881 cgcgggtaag tccaccctgc tgcgcattat ggcgggcatt gataaagaca tcgaaggtga
    29941 agcgcgtccg cagccagaca tcaagattgg ttatctgccg caggaaccgc agctgaaccc
    30001 ggaacacacc gtgcgtgagt ccattgaaga agcggtttca gaagtggtta acgccctgaa
    30061 acgcctggat gaagtgtatg cgctgtacgc cgatccggat gccgattttg acaagctggc
    30121 cgctgaacaa ggccgtctgg aagagatcat tcaggctcac gacggtcata acctgaacgt
    30181 acagctggag cgtgcggcgg atgcgctgcg tctgccagat tgggacgcga aaatcgctaa
    30241 cctctccggt ggtgagcgtc gtcgcgtggc gttgtgccgc ctgctgctgg aaaaaccaga
    30301 catgctgctg ctcgacgaac cgaccaacca cctggatgcc gaatccgtgg cgtggctgga
    30361 acgcttcctg cacgacttcg aaggcaccgt ggtggcgatt actcacgacc gttacttcct
    30421 cgataacgtt gcaggctgga tcctcgaact ggaccgcggt gaaggtattc cgtgggaagg
    30481 taactactcc tcctggctgg agcagaaaga tcagcgtctg gcgcaggaag cttcacaaga
    30541 agcggcgcgt cgtaagtcga tcgagaaaga gctggagtgg gtacgtcagg gaactaaagg
    30601 ccgccagtcg aaaggtaaag cacgtctggc acgctttgaa gagctgaaca gcaccgaata
    30661 ccagaaacgt aacgaaacca acgaactgtt tattccacct ggaccgcgtc tgggcgataa
    30721 agtgctggaa gtcagcaacc tgcgtaaatc ctacggcgat cgcctgctga ttgatgacct
    30781 gagcttctcg atcccgaaag gggcgatcgt cgggatcatc ggtccgaacg gcgcgggtaa
    30841 atcgaccctg ttccgtatga tctccggtca ggaacagccg gacagcggca ccatcacttt
    30901 gggtgaaacg gtgaaactgg catcggttga tcagttccgt gactcaatgg ataacagcaa
    30961 aaccgtttgg gaagaagttt ccggcgggct ggatattatg aagatcggca acaccgagat
    31021 gccaagccgc gcctacgttg gccgctttaa ctttaaaggg gttgatcagg gtaaacgtgt
    31081 tggcgaactt tccggtggtg agcgcggtcg tctgcatctg gcgaagctgc tgcaggttgg
    31141 cggcaacatg ctgctgctcg acgaaccaac caacgacctg gatatcgaaa ccctgcgcgc
    31201 gctggaaaac gccctgctgg agttcccggg ctgtgcgatg gttatctcgc acgaccgttg
    31261 gttcctcgac cgtatcgcca cgcacatcct ggactaccag gatgaaggta aagttgagtt
    31321 cttcgaaggt aactttactg agtacgaaga gtacaaaaaa cgcacgctgg gcgcagacgc
    31381 gctggagccg aagcgtatca agtacaagcg tattgcgaag taatgcgtaa aatgccggat
    31441 gcggcgcgaa cgccttatcc ggcctacaaa accagcaatt tcaatacgtt gtaaaactgt
    31501 aggcctgata agacgcggta agcgtcgcat caggcattga gcaccgaatg ccggatgcgg
    31561 cgtgaacacc ttttccggtc tacaacagcc ttagcatcct caatttcgtg gcgggtcacc
    31621 cgtgcaggtt ttttcaaacc ttgtttaatc tcgacagcct cttccagaga ggttttcaac
    31681 tcatcaaaaa aactcatttt atacctcgcc tttgagcagt ttcgtcacgg ttatccttgc
    31741 tcccccatca tctcccgcac cagctccacg cagcgcagga aacggctgtc gtaatcgtcc
    31801 tcttcaaccc gcacgaattc gatattgttc tcttccagca tctccaccag caagttctgg
    31861 aactctttgc gatccaccga actgccgagg ctgcgtaaac catccgccac ccacggcgtg
    31921 ttgttctcca gcaggatcac cagatcgaaa cggtattcat caatcagcgc ctgcacgaac
    31981 ggatgctcac gcccttcgta ctttttgcag aacgcctggg tagtgacaaa atcggtgtca
    32041 ataaacgcca ctttattggc gtatttcact gcaaaatcaa tgtattgtgc gtggcccaac
    32101 gcgattttat cgtagtcgga atactgcaat gcgatctcat caccgccgag gtgtgaaaag
    32161 acataatcgc ggccatattc ccacgcactg gtggtgttga agatattggc aagtttgttt
    32221 accagggtgg atttaccgct cgactcgccg ccaaggatcg ccacggtacg cacaaaaaac
    32281 ggcttcactt cggtaggaat atattcccag tagcggaacg ggttttcgcg gatctgcgca
    32341 ccgctgatac tcataaaggt acgtttcgga tcgaccagca ccgtctcgat ccccagatgt
    32401 tccatatact gtggcgcatc ggcttcttcc gaggtgtaga tcaggtccgg ctggatcccc
    32461 ttttcagcca taaacttttt gatgccgttg ctccacacat cccagccatg cggatacggc
    32521 tccatgccct cttcgttgaa agcatgaata cggatatttt tctgatattt aaaggtttgc
    32581 aatagccagc gcagacgatc cggcaccgtg ggctgctgcg acatggcgct gtcttcaaac
    32641 aacgcgcggt cgcgggtatc gtcaaaaccc ataatgatat gcagctcgtc aacctggcta
    32701 caggcgcgct ggataaggta gatatgtccg gtatgcagcg ggtagaactt accgaatacg
    32761 acaccaatgg ttttcttctg ccggggaaat tcaagcccca aaaaacggtg caatgcctcc
    32821 agcttttgcg cgctggggct tttaattttg gcattcagta actggcttaa ataccctttg
    32881 gtcataccgc tggcatcagc tacctgctgt agcgtgcagc cctgttgctt gatggcagtt
    32941 ttcaggtaat caaatgacga catatctccc tccgtatctc tcattataag tcgtcgaaca
    33001 cgctaagcgc gtcggagagt tttttaacgc caaaaatctg catcccttcc ggcgcttttt
    33061 tcggtacgtt agccgccgga acaatcgccc ggcgaaaacc gtgtttcgcc gcttcagaaa
    33121 tccgctcctg gccactgggc accgggcgga tctcccctgc cagcccgact tcaccaaaca
    33181 ccaccagatc ctgtggcagc ggtctgtcac gcaggctgga aaccatcgcc agcagtaacg
    33241 ctaagtcggc gctggtttcg gttaccttca cgccgccgac cacgttcaca aacacatcct
    33301 gatcggccat ttgcagacca ccgtgacggt gcaagacagc cagcaggatt gccagacggt
    33361 tttgttccag ccccactgcc acgcggcgcg gattcgccat tatcgagtga tcgaccagcg
    33421 cctgaatctc caccagcaac ggacgcgttc cttcccacac caccatcacg gagctgccgg
    33481 aggtcacttc atctccgcga cttaagaaaa ttgccgaagg gttgctgact tcacgcagcc
    33541 cctgttcggt catcgcgaag acgcccagct cattcaccgc gccgaagcgg tttttatggc
    33601 tgcgtaaggt gcgaaaacga gagtcggcat cgccatccag aagcaccgaa cagtcgatac
    33661 agtgttccag cactttcggg ccagccagcg aaccatcttt ggttacgtgt ccaaccatga
    33721 caatcgccac accgcgcgtt ttggcgaaac gcgtcagata agccgccgtt tcacgcacct
    33781 gcgccacgct accaggcgat gattgtacat ccgccatatg catcacctgg atcgagtcaa
    33841 ttaccatcag cttcggttgc tcttcttcgg caatcaggca gatctgctcg atgctggttt
    33901 ccgacaacat attgagattg tcagtcggca ggccaaggcg atgagcgcgc attgccacct
    33961 gttgcagcga ctcttcgccg gtgacataca gcgttttcat ctgctgggcc agtttgcaca
    34021 gcgtttgcag caacagcgtg gatttccccg caccagggtt accgccaatc agaatggcac
    34081 ttcctggcac cacgccgccg cctagtacgc ggtcgaactc tttaaatccg gtggaaaaac
    34141 gcggcagctc ttcaaggctg atatcggaga gtttctggac ttttgccacc ccggcgctac
    34201 cggcatagcc gctgagacgc tcgttacgcg ccaccgttgg cgacgcagca agacgcacct
    34261 cggtgatggt gttccaggca tgacaggcac tgcactgccc ctgccagcgc ggataatcgg
    34321 ccccgcattc attacaaaca aaggcgcgtt ttggagcttt tgccacggtg tacctcgtta
    34381 atgctgtgcc gcccgcagga tgacgggcag gcaattactt ctgattcagg ctgccagaga
    34441 ggatgcagaa tacccccatc aggtcagcgt gacggatggt gacttccgcc ttttcattca
    34501 cttttggctt ggcatggtag gcaatcccca gccctgctgc tttgatcatc ggcaggtcat
    34561 tagctccatc gccaatcgcc acggtctgcg ccagcgggat ttcatactcc tgcgcgaggc
    34621 gagtcagagt tttcgctttg tactgtgcgt ctacgatgtc gccgatcaca ttgccggtaa
    34681 atttaccgtc catgatctcc agttcattgg ctaccacggc tgtcaggcgc agcttgtcgc
    34741 gcaggtattc agcaaagaaa gtaaagccgc cggaggcaat cgccactttc cagcccagcg
    34801 tttccagctt gagcaccagc tgcgttaagc ctggcatcag cggcagattt tcacgcacct
    34861 gttgcagaat attggcgtca gcgcctttca gcgtcgccac gcggctgcgc aggctggcgg
    34921 taaaatcgag ttcgccgcgc atcgcccgtt cggttacctc cgctaccatc tcgcccgttc
    34981 cggccagctt ggcaatttca tcaatacatt caatctggat ggcggtggaa tccatgtcca
    35041 tcaccagcaa acccggcgtg cgcaggtgcg ggattttccc cagcggggcg acatccagct
    35101 gcgcttcgtg ggccaggcgt gtagcccgtg cggtgagtga acctgccaga cgaatcacct
    35161 gataatcttc cacgcaccag gcggcaacaa tcaccatcgc cgcacccagt ttgctctggt
    35221 attgggtcag acgctgttta tccagcccac gaccatacag cagccagccg ctacgagctg
    35281 cgtggtaatc cagtggcatc acttcatcac cacttaatga aagaggcaga cccggccata
    35341 aagagacatc ttcaggcagg tcgcaccagg taatgttagg cattaaggct cctgtaaaat
    35401 cgttcgaagc agggaaaata acgcatgagg ctaccttgta tccattgctt ctggcaacat
    35461 taagtctcaa attttcaaag ggtggaagat ggctcgcaca aaactgaaat tccggctaca
    35521 tcgggcagtg attgtcctgt tctgtcttgc cttgttagtg gcactgatgc agggagcgtc
    35581 atggtttagt caaaaccacc agcgacagcg taatccacag ctggaagaac tggcccgcac
    35641 cctggcgcgt caggtgacgc tgaacgttgc accgctgatg cgtaccgact caccggatga
    35701 aaaacgcatt caggcgatcc tcgatcagtt aacggatgaa agccgtatcc tcgacgcggg
    35761 tgtgtatgac gaacaaggcg atcttatcgc acgttctggc gaaagcgtcg aagtgcgcga
    35821 tcggctggcg ctcgacggta aaaaagcagg cggctatttt aaccagcaga ttgtcgagcc
    35881 aattgcgggt aaaaacggac cgctcggcta tctgcgcctg acactcgaca cccatacgct
    35941 cgccaccgaa gcccaacagg tggataacac cactaacatt ttacgcctga tgttgctgct
    36001 ctcactggca atcggcgtag tgctgacccg cacgctgcta cagggtaaac gcacccgctg
    36061 gcagcaatcg cccttcctgt taaccgccag caaaccggtg ccggaagagg aagaaagcga
    36121 gaaaaaagag tgacccatta ctacaagaaa ggaaatcgtt atgtccacat tacgcctgct
    36181 catctctgac tcttacgacc cgtggtttaa cctggcggtg gaagagtgta tttttcgcca
    36241 aatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat gccgacacgg tagtaattgg
    36301 tcgcgcgcag aacccgtgga aagagtgtaa tacccggcgg atggaagaag ataacgtccg
    36361 cctggcgcgg cgcagtagcg gtggcggcgc ggtgttccac gatctcggca atacctgctt
    36421 tacctttatg gctggcaagc cggagtacga taaaactatc tccacgtcga ttgtgctcaa
    36481 tgcgctgaac gcgctcggcg tcagcgccga agcgtccgga cgtaacgatc tggtggtgaa
    36541 aaccgtcgaa ggcgaccgca aagtctcagg ctcggcctat cgcgaaacca aagatcgcgg
    36601 cttccaccac ggcaccttgc tactcaatgc cgacctcagc cgcctggcaa actatctcaa
    36661 tccggataaa aagaaactgg cggcgaaagg cattacttca gtgcgttccc gcgtgaccaa
    36721 cctcaccgag ctgctgccgg ggctcaccca tgagcaggtt tgcgaggcca taaccgaggc
    36781 ctttttcgcc cattatggcg agcacgtgga agcggaaatc atctccccgg acaaaacgcc
    36841 agacttgcca aacttcgccg aaacctttgc ccgtcagagt agctgagagt ggaacttcgg
    36901 tcaggctccg gcattctcgc atctgctgga tgaacgcttt agctggggcg gcgtggaact
    36961 gcatttcgac gttgaaaaag gccatatcac ccgcgcccag gtatttaccg acagcctcaa
    37021 ccccgcgccg ctggaagccc tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat
    37081 gctgcaacaa gagtgcgaag cgctgttggt tgacttcccg gaccaggaaa aagagctacg
    37141 ggagttgtcg acgtggatag cgggggcggt aaggtaactc gctgattaaa atatccaagg
    37201 aaggaaatat gatcgatctg gaaaaccagg aacgggagat tatcaattta atgttcagcc
    37261 agcgtatttc ctggctagcc gccgtgcgta tcaggcataa attatcgctt gctgaagtaa
    37321 gtaaaatgtt aggtatctcg attaattctc taaaacagat cgaaaagacg gaacggctta
    37381 gctcaaacat caaaagcaaa atggcagaaa tttatggttg tccaccagag ttgctgatat
    37441 gcccttcctg gatgacggct gaacataaat gaatagattc gtgaatagat acttgaatgt
    37501 attttatttt ctatccatat gaaaaataat agtaaatcta taaatcagtt gctattgatg
    37561 aatagatact aaaaagccgg agcagtcccc cactccggct taacacgaca atcgcctttc
    37621 agcgaaacac aattactctt tatcgcccag cagaacggat tccagtgcga ttttgatcat
    37681 gtcgttgaag gtggtctgac gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc
    37741 agacacggtg cagatggtca gggctttcgc gccaaactct gcagccacgc cgtagatacc
    37801 agccgcttcc atttccacgc cgaggatgcc gtatttttcc atcacgtcga acatttcgcc
    37861 gtccggagag tagaacaggt cagcggagaa caggttaccc acgcgagcat caacgcccag
    37921 tgctttagct gcatctactg cgttacgcac catgtcaaag tcagcgatag cggcaaagtc
    37981 atggtcttta aaacggatgc ggttaacttt ggaatcggtg caggccccca taccgataac
    38041 gacgtcgcgc agttttacgt gcggcagaac tgcgccacag gaaccaacgc ggataatttt
    38101 cttcacgccg aaatcggtga tcagttcttt ggtgtagatg gagcaggacg ggatacccat
    38161 accgtgaccc attacggaaa ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc
    38221 gcgaacgttg ttcacttcac gggcatcttc aaggaaagtt tcagcaatat acttcgcacg
    38281 cagcgggtcg cctggcatca aaactacgtc agcgaaatcg cccatttctg cattaatgtg
    38341 tggggtagcc attgttttat cctttttgtg acataacaaa ggcggagtca acgctccgcc
    38401 caaatccatc agaacatggc tttgccatat tccatatcag aagtaccaaa atattttgcc
    38461 agagtctggc cgatatccgc gaaggtttca cggtgaccca gtgagcccgg ttttactttc
    38521 gggccatata ccagtaccgg aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga
    38581 tcgcaaccgt ggtcagcggt gaggatcagg atgtcgtcat cgcgcagcag agacatcagc
    38641 tccggcagac ggcggtcgaa cagttccaga cccgcagcat aaccggcgac gtcgcgacgg
    38701 tggccccagg aagagtcgaa gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct
    38761 tctttcatct ctttgatggt ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt
    38821 ttggtgatac cgcagttggc gtagatgtct gcaattttac cgacagaaac cacctggccg
    38881 tgtttttcat caaccagttt ctgcagcacg gtcggtgctg gcggctcaac agccaggtcg
    38941 tgacggttac cggtacgctg gaagttacca gctttgtcac cgataaacgg acgagcgata
    39001 acacgaccga tattgtagcc gccgttggtc agctcttcac gggcgatttc gcacagttcg
    39061 tagagtttat ccagaccgaa agtttcttca tggcaggcaa tctggaacac ggagtcagcg
    39121 gaggtataga aaatcggctt gccggttttc atgtgctctt cgcccagttg atccagaatg
    39181 accgtaccgg aagagtggca gttaccgagg taacccggca gattagcgcg ttcgaccagt
    39241 ttatccagca gctcttgcgg gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac
    39301 agaaccggga caccggcgat ttcccagtga ccagacgggg tatctttacc ggatgacatt
    39361 tcgtgcgccc atgcgtacgc gccgataact tcagcgttgc cgtccattcc cgccggaatg
    39421 aaaccggtag aaccttcgtg tgctttcgcc agccccagac ggatcagatt tggcagattg
    39481 agcggccctt tacgaccatt atcagcttcg cctttggcac aagcttctgc gatatgaccc
    39541 agggtgtcag ccccgacgtc accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat
    39601 gagtccagca ccataataaa tgcacgtttc atatgttctc cgtactcagt gcttcaaatt
    39661 taaatgatca gatcagtata ccgttattcg ctgatacggc gatagacagt tggtgtgctt
    39721 tccggtgcgt tatcggcaag tttaattgcc gctttcaccg ctttcgccgc ttcctgccag
    39781 ctgttttcgt ctttcgcgag gataaccgcc agcggacgct gaccgtctac ctggtcgccc
    39841 agacgcgcca tatcagtaaa gccgacgctg taatcgatgg tgtcagatgc ctgacgacga
    39901 ccgccgccca ttgcaaccac tgccatcccc agcgcgcggg tatccatttc actgacaaaa
    39961 ccttcggtat cagcatagac tgctttcgtc agcatcgctg tcggcagata cttcgcgtag
    40021 ttctcaacga agtcggtcgg gcctttttgt gccgctacca tacgaccaaa gacttctgcc
    40081 gctttaccgt tgtccagcac cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc
    40141 agtttgccgg agatcagcat ctccacgcac agcaccatcg tgacatcaaa cagacgcggg
    40201 ttacgatact cacccgtcag gaactgcacc gcttcacgaa cttcaaccgc gttacctgca
    40261 ctggaggcca gtacctgatt catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg
    40321 ttagccacgc caacaatcgc ttcggcaagg gcttcagaga gttcgtaggt cggcataaac
    40381 gcgccgctac ccactttcac gtccatcacc agcgcgtcca gaccttccgc cagtttcttc
    40441 gccaggatcg aggcagtgat cagcgggatg gagtccacgg ttgcggtaat atcacgggtc
    40501 gcgtagaaac gtttatcagc cggagccagt gaactggtct gaccgataat cgccacgccg
    40561 acgtctttaa taatttcgcg gaaacggttg tcatccggga aaatgtcgaa gccagggatg
    40621 gattccagtt tgtcgagcgt accgccagta tgaccgaggc cgcgaccgga gatcatcgga
    40681 atatagccgc cgcaggctgc gaccatcggc cccaacatca gcgaagtgac atcgccgacg
    40741 ccgccggtcg agtgtttatc aacaatcggg ccatccagat gcaggctttt ccagtcgaga
    40801 acggttcctg aatctcgcat cgccatggtc agcgagacac gctcaggcat tgtcatatcg
    40861 tggaagaaaa tggtcatcgc gagggcggca atctgccctt cggaaatagt gttgtcgcga
    40921 ataccgttga taaagaaacg aatttcttca tcgctcagcg catgaccatc acgtttttta
    40981 cgaataattt cttgtgcgag aaacaaggta acctcccgag gaaaagagta tgaggcggct
    41041 tttgccggat gcgtctacgc cttatccggc ctacgggtag atacgtctcg taggcctgat
    41101 aagcgcagcg catcaggcgt aaagcatctt acttagtagc tgctggcgct cttaccgtcg
    41161 ccgtggccca gcgctttcag caggcttgcc agcaggctgg aagcaccaaa gcggtagtga
    41221 cgcgcatcag cccagtcagc accgaacagt tcatctgcga tggcgagata tttctgcgca
    41281 tcttccgcag tacgcacgcc gcccgccggt ttgaaaccaa cggttttttc tacgcccata
    41341 tcacggatca cttccatcat gatgcgagcg ctttccggcg tcgcgttcac agccacttta
    41401 ccggtagagg ttttgatgaa gtccgcaccc gctttgatgg agatttcaga cgctttacgg
    41461 atcagcgctt cgtctttcag ttcgccggtt tcgatgatca ctttcagcag tacattcgct
    41521 gccgcgcaag cctctttaca ggctttcacc aggtcaaaac caacctgctc gtcacccgcc
    41581 atcagcgcgc ggtacgggaa taccacgtca acttcatcag caccgtaggc gattgccgca
    41641 cgggtttctg ccagcgcgat ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta
    41701 gcgatacgga tttccggggt gccctgctct ttcagagttt tgcgagcaat cgggataaaa
    41761 cgaggataga tacagatagc ggcggtattg ccgaccggag ttttggcctg atgacacaga
    41821 gcaattactt tctcgtcggt gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt
    41881 gcacgcaggc tgcttgcttt cagatcagtc atttcattct cctggcttgt cgccaataaa
    41941 attcaccttg cgagtttgtt agtattctaa catctactcc gcaacacact tcgatacaca
    42001 tcacaattaa ggaaatctac ttacaagttt gcatcactgt aatgcgatct ggttcaaata
    42061 attcactttc aaatgaatgc gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca
    42121 cgaaacttct tacaatggcg catcaccttc ggcataaaag gaaaagaaga tgcccacttc
    42181 tcatgaaaat gcactgcaac aacgttgcca gcaaattgtc accagcccag tgcttagccc
    42241 ggagcagaag cgccattttc tggcactgga agcagaaaac aatctgcctt acccacagct
    42301 tcctgccgaa gcccgccgcg cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca
    42361 tgcgccgtac aaaccgcgct atgtcttacc cgattacgct cgttttctgg cgaacggttc
    42421 cgaatggctg gagctggaag gcgcgaaaga tcttgatgac gcactctctc tgctgaccat
    42481 tctttaccac cacgtaccgt cggtcacatc gatgccggtc tacctggggc aactggatgc
    42541 gttgttgcaa ccgtatgtta gaattctaac acaaaacgag atcgatgttc gaataaaacg
    42601 tttctggcgt tacctcgaca gaaccctgcc agatgccttt atgcacgcca atatcggccc
    42661 gtctgattcg cccattaccc gcgcgatctt acgtgcagac gcgcaggttt caccgaacct
    42721 gacctttatc tacgatcctg aaatcacccc tgatgacctg ctgctggaag tggcgaagaa
    42781 catctgtgaa tgtagcaaac cgcacatcgc caacggtccg gtgcatgata aaattttcac
    42841 aaaagggggc tacgggattg tgagctgtta caactcactg ccgctggcgg gtggtggcag
    42901 cacgctggta cgccttaacc tgaaagccat tgccgagcgc agcgaatcgc tggatgactt
    42961 ctttacgcgc actctaccgc actactgcca gcagcagatc gccatcatcg atacgcggtg
    43021 tgaattcctc tatcaacaat cacacttctt tgagaatagc ttcctggtga aagaagggct
    43081 gattaaccct gaacgttttg tgccaatgtt tggcatgtat gggctggcgg aagcggttaa
    43141 cttgctgtgt gagaaagaag ggattgccgc gcgctacggt aaagaagccg ccgcaaatga
    43201 agtgggttat cgcatcagcg cgcaactggc ggagtttgtc gccaataccc ccgtgaaata
    43261 tggctggcaa aaacgcgcca tgttacacgc acagtcgggg atcagttccg atatcggcac
    43321 cacgccgggc gcgcgtttgc cgtatggcga tgagccagat ccgatcaccc atctgcaaac
    43381 tgtcgccccg catcatgctt attattattc cggcatcagc gacattctga cgctcgacga
    43441 aaccatcaaa cgtaacccgc aggcactggt acagctttgc ctcggtgcct ttaaagccgg
    43501 aatgcgtgaa tttaccgcca atgtcagcgg taacgatctg gttcgcgtta ccggttatat
    43561 ggtgcgtttg tcggatttag aaaaatatcg cgccgaaggt tcacgcacca acaccacctg
    43621 gctgggcgaa gaagccgcac gcaacactcg tattctggaa cgccagccgc gcgtgataag
    43681 ccatgaacag cagatgcgct ttagtcagta agattatccc cttctcctgc gttgacgggc
    43741 caggcagtcg tctggctctg tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca
    43801 atccgtggac gatgggacgt tgcaatgact gtggggagtg cgtgccacag tgtccgcatc
    43861 aggcgttgca gattgttgac ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg
    43921 atacctgcct gaagatgtgt ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg
    43981 acgaagtgct tagccatgtg cgcaaagcgg tgctgtttat tgaagggata acggtaagcg
    44041 gcggcgaagt cacgacccaa ctgccgtttg tagtggcgct gtttactgct atcaaaaacg
    44101 atccgcaact gcgccatctc acctgtctgg tggacagtaa cggtatgttg agcgaaaccg
    44161 gctgggaaaa attactcccg gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta
    44221 gcgaatgtca tcaacaatta accggacgcg ataatcaaca gattaagcgc agcatctatt
    44281 tgctggcaga gcgcggcaag ctggcggaac tgcgtttgct gatgatccca ggccaggtgg
    44341 attatttgca acacatcgaa gaactggcgg cgtttatcaa gggacttgga gatgttccgg
    44401 ttcgcctgaa cgcgtttcat gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg
    44461 ccacgccgga agacgttgag ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt
    44521 tgatatttcc ggcgctctat ttgtgacatg ccggatacgg cctacggttc ggcacagact
    44581 tgtaggcctg ataagacgcg tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg
    44641 cggcgtgaac gccttatccg gcctacggtt cggcacaaac ctgtaggcct gataagacgc
    44701 gttaagcgtc gcatcaggca ttatgcgccg actgccggat gctgcctacg aagggcaaac
    44761 tacccacgaa cgttaaacac cgcatacgtg ttattaagca acacttcggc aatctcatcc
    44821 gcgggttccg ggcgtaactc gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg
    44881 ttaggctgcc cctgaaaacc gttgagcggc atatccggcg catcggtttc cagcaacaac
    44941 gatgccagcg gtaattttgc gatgacatcg cgggttttac tggcgcgtgg ataggtgata
    45001 gtaccgccta cgccgatttt gtagcccagc tgtacaaacc gttcagcctg ttgctggctg
    45061 ccagaaaatc cgtggacaac accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc
    45121 agtttgtcgt gcgtgcgccg tgaatgcagg atcaccggca gatcgtagcg tttcgccagt
    45181 ttcagttgtt cgtcgagtaa ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga
    45241 tccagaccga tctcccccac cgccaccacc ttcgccggac gcctttccag cgcctgctgt
    45301 agctgctcaa gagacacatc gctatgtttt tccaacatac caggatgcaa gcccaatgcg
    45361 gcatacagcg gttgataatt ttccgctaac gcctgcacgc gggcaaaatt ttccgcctct
    45421 gtcgccggaa caatgatctt gcctacgccc gcttgtgccg cgcgttgcag gctggcctct
    45481 tcatcgccaa taaaaggcgg gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc
    45541 aagccagatc ctcattgtta aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg
    45601 caaccagcgt atcgttggcg actggcgcag gcggaatgac aatcgattcc ggcgtcacca
    45661 ctggcaccag atggcgggta agcggcgctt tttcagttaa tagcttgcca accgtggcga
    45721 ggaaataacg accgcataaa cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac
    45781 tgccaagcgc gatactatgt aatggcttcg gcggataaat ttcggatatc cgcagcttgc
    45841 ccggtggttt ctcaataaat tgctgaatgt cacgatagct ggtttcatga tgctgcacca
    45901 gattgaccag cggctgcagg ctactgtcac ccagccagcg ttccatgcgt ttgaaccact
    45961 gcggcgtgta gtacatttgt gacggcacag tgcggatgac gaccaacgtt ttagcgccct
    46021 gccttgccgc ctctttaacc ggaatcgcat cactgatccc gccatccagg tagttaatgc
    46081 cttccagcga cactccgcta cgataaaagc caggtatcgc actggaggcg cgaatcacat
    46141 ccagccagtt ttgtttggtt ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg
    46201 cgcacatata aaacgatttg ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg
    46261 gcatctggct tgcggtggcc tccaccagcc agtcgagatc gataagattt cctccacgga
    46321 caaagcgcaa tggatcaaaa aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg
    46381 cgtaaccggg ctgattgcag ataaacgccg agaggttctg cgccccggca gatgtgccga
    46441 gataaagatc gaaaggatta aactgcgcgc gcataaactc atccagcacg ccagccgtga
    46501 aaattccacg ctgtccgccg ccttcgcaca ccagagctat tcgtccaggc tggaacggcc
    46561 ttagcgacaa cggcgcaata ttaccaagcg ttacaggtat tctctgcccc acttctgcct
    46621 tcctgttttt gttgtgaaag aaagacaaag taacgcaatt tattattcgc taaaaccgca
    46681 aaagccagtc cgctggactg gctttggata aaattttctt aatggtctga aatctagggt
    46741 cgttttcggc ccatgaacaa actcactagg aacagaataa tcccgacgac aaagacaatt
    46801 ttagctgcgc ctgcagcggt accggccaga ccaccaaacc caagtgcggc ggcgattaac
    46861 gcgataacca gaaatatgat gccccaacga aacataagcg tctcctttac catagttaat
    46921 gtcaccgctt gatatgagcg tgaaaatcac tcaatgcgat atttttagtg tggtgcacat
    46981 cgcgcctccc gacaaagttc gggaggacga attacgacga attacttagt tttcagatca
    47041 tttttaacgc ttttcacacc atctaccgct ttggcgatac tttcagcacg gtcactttgt
    47101 gcctgagaat ctacggtacc ggagagctga accacgccat cggtggtttc aactttcact
    47161 ttacgtgaag ggacgatatc gtccgccagc agtttggctt tgatttcact ggtggtggcg
    47221 gtgtcacccg cgtagtcctt caccgagcct tctttagcgt cgcgaacgtg cagtttgtcg
    47281 ctgacagagg tcacgccttc aacgcctttc gccactttca ctgcctcttc ggcctgggcc
    47341 tgactttcaa cgaaaccgct cagggtcacg actttttgat cggttttgac agagatatcg
    47401 gtgctcttga tgttgtcatg atccaccagc gccgccttca ctttcgcggt gatggcgctg
    47461 tcatccatga aattaccgac tttattcata gagctatcga ctttttgccc tgcgctttca
    47521 ttggtagtct gcgcgttgtt ttccgcgtag gcagagccgg tcgcgacggc agaggtcaac
    47581 attacagcca gcagagtttt cgaaatcttc agtcttgtca tagtcatcga tttattcctg
    47641 tatgtttgct cgtaatttga gcctagcaac acgaggttgc attgctgaat agggagagac
    47701 ttcaccctct acagaagtca atggtcgcca tcacaaaagc gatgggtgat gaataacgac
    47761 cattacagtc tctgaatcag ttattaatat cggtagaatg acaatcgacg gctttagata
    47821 ctgatatcta cgcattgaac ggtatttaac gccatcagaa atgtcatcac tttgttaaat
    47881 atagatcaca attttgaaac cgctcgggat atcagcgaaa acataagcaa aagtgaatgt
    47941 tttaagaaca ttccgtaagc ggctaataag gaagggaaat tgacagggcg cagcggctac
    48001 cgcgccctgg aggcaagaat taatgctcgc gggtctggtg gaactgaacg tccggataac
    48061 gttcctgtgc caggcgcagg ttgaccatgc tggtagcgat gtaagcgagg ttatcgccgc
    48121 catcaagcgc cagttggctt tcgttcttac gcttgaactc ttcgaatttc ttcgcgtctg
    48181 cacattctac ccagcgggca gtggcaacgt tgactgactc atacactgct tcaacgttgt
    48241 attcgctctt caggcgcgct accaccacat caaactgcag cacaccaacc gcaccaacga
    48301 tcagatcgtt gttggagatt ggacggaaca cctgcaccgc gccctcttcg gaaagctgta
    48361 ccagcccttt gagcagctgt ttttgcttca gcggatcttt caggcggata cgacggaaca
    48421 gttctggtgc gaagttcgga ataccggtga acttcatcat ctcaccctgg gtaaaggtgt
    48481 cgccgatctg aatggtgccg tggttgtgca ggccgaggat atcgcccgga tacgcttctt
    48541 caacgtgcga acggtcaccc gccataaagg tcagcgcgtc ggagatcacc acatctttcg
    48601 cagtgcgcac ctggcgcagt ttcatgcctt tttcatattt accggacacc acacgcataa
    48661 acgccacgcg gtcgcggtgt ttcgggtcca tgttggcctg aattttaaat acgaagccgg
    48721 taaatttatc ttcgctcgct tctacggtac gggtatcggt ctgacgcggc atcggcgcag
    48781 gcgcccactc caccaggcca ttcagcatat gatcaacgcc gaagttaccc agcgcagtac
    48841 cgaagaatac cggagtgatt tcgcccgcaa ggaacagctc tttgtcgaac tcgttagacg
    48901 cgcctttaac cagttccagt tcgtcacgca gctgctgtgc cagatcttca ccaaccgcag
    48961 catcgagatc cgggttattc agccctttaa caatgcggac ttcctgaatg gtgtgccctt
    49021 taccgctctg atagagatag gtttcatctt tataaaggtg gtaaacgcct ttaaacagct
    49081 tgccgcagcc aattggccag gtgatcggtg cgcagccaat tttcagctcg ttctcaactt
    49141 catcgagcag ttccatcggg tcgcggatat cacggtcaag tttgttcata aaggtgagga
    49201 tcggcgtgtc gcgcagacgg gtaacttcca tcagcttacg ggtacgatct tcaacacctt
    49261 ttgcggcgtc gataaccatc aggcagcagt ccaccgccgt cagggtacga taggtatctt
    49321 ccgagaagtc ttcgtgcccc ggggtgtcga gcaggttaac caggcaatcg tgatacggaa
    49381 actgcatcac agacgtagta atggagatcc cacgctgctt ttccatctcc atccagtccg
    49441 acttagcgtg ctggttggaa ccacggcctt ttactgtacc ggcggtctga atggcctgtc
    49501 cgaacagcag caccttctcg gtgatggtag ttttaccggc gtccgggtga gaaataatgg
    49561 caaaagtgcg gcgcttcgcc acctcttgca aataaggaga caacgtcata attctttctt
    49621 cttgagtaag cggcatcgcg ccgcgcttgt tgaaaacgaa aaattgcggc tattttaccc
    49681 atcaacgggg gggaggcaat cagtgtttac acaggagctg ctccagttcg tgcaacgaag
    49741 aaacggtcca ggtgggcgcg atgccttctg gttgctcgcg attgtgcgca ttcagccagc
    49801 aggtcgcaag cccggcgttg atgccaccga gaatatcgga ctcggcagtg tcgccaacca
    49861 tcagcacgcg tgaacggtca ggattgcccg cctgttccag cgcataatcg aaaattttct
    49921 tattcggttt ggcaacgcca acttcttcgg aaatcaccag caaatcgaag taatcacgca
    49981 ggcccgtccg ttccagacgc acttgctgca aggcgctaaa accgttggtg atgatgccga
    50041 ttttagcgtt gccacgaatg gcgttaagca gagaaaccgc gcccggcaac ggcgtacaga
    50101 tttccgccat cgcattaata aaggcatcgt taagcaagcc tgcttcaact ttcagccgtt
    50161 cggcccagct ctcgaaacgc ccgtgctgaa gctgtaatga agtgatcgcg ccgttttgat
    50221 aatccaccca cagtggcttg ttaacggcct gatagtcctg aaaatcttca gcggtaaagg
    50281 tgacgctgta atcaagaaac atccgctgca ggccggtgaa tgagtcaaag gtaaacagcg
    50341 tttcatcggc atcaaagaaa atccagtccc acttcattat tccaccttgt cttacatact
    50401 gattggcaac gccatgatga tggcgtcttc gcgaccgtcc gtggtggggt agtaattgcg
    50461 gcgaatcgtc gcctcgttaa agcctaaact ttcgtacagg gcaatggcgg cagcatttga
    50521 ggcacggact tccagccata gtgtcgccac gccgcgtttt tccagttcgt cgatcagatg
    50581 ttccagcagc gccctaccca gcccctgacg ctgatagtcg ggatcgaccg caatattgaa
    50641 caatgtagct tcatccagca ccacttgcgt aatcgcaaac gccgccattt tgccgttttg
    50701 cgttaactga aagttgagat aacgctcgcc ctggttgctg gcaaaggttt tttcactcca
    50761 cggaaaggcg tgggcgcgtt gttcaatgtg gtaagccgct ggtaaatcag tcgtttcgag
    50821 ggaagaaatc gtgttcatat gtgcaaattt gttgccataa cgcggcgcgt gccgttgggt
    50881 ttgcccgtaa ttcggtgagc gccggtgatg ccacctgagc gccttccaat gatagcggtt
    50941 cgtcagtacc caaccgccag ctgttgcagc gactgccttg cggcagcatc gcgatttttt
    51001 ctggcgtcag ttgcagcact tgatcggggc tgacggttaa tgcgcgcaga acatcgctca
    51061 ctaaaggatc agtcagggcg ggaagatcgt ttgccaccat caccagacgg acgtgtgccg
    51121 ggctcgcaat ggcaatctcc ccctgcaacg cgccagggcg acgcagcgac cactgggtaa
    51181 tgcccagttg ctgtaactgc cagtctcgtc gggatgtcat agcgaatcgc tcctgttgtc
    51241 aggggcgcaa atatagcaaa ttcgtcgata ccgcgccaac atatggctat aatcgccgcc
    51301 agtatcaatt gaggagcatt ccatgtctgc atttaccccg gcaagtgaag tcttgctgcg
    51361 tcacagtgat gatttcgaac aaagccgtat tctgtttgcc ggagacttac aggatgacct
    51421 gcccgcgcgt ttagataccg cggccagccg tgctcatacc cagcaattcc accattggca
    51481 ggtgttaagc tgccagatgg gggataacgc ccgttttagt ctggtcgcca cggcgaatga
    51541 tgtcgcagat tgcgatacgc tgatttacta ctggccgaag aacaaaccgg aagcccagtt
    51601 ccagttgatg aatttacttt ctctgctgcc agtggggaca gatatttttg tcgttggcga
    51661 gaaccgcagc ggcgtgcgca gcgctgagca gatgctggca gattatgcgc cgttgaataa
    51721 agtcgacagc gctcgtcgct gtggcctcta ttttggtcgt ctggaaaaac agccagtatt
    51781 tgatgcggat aaattctggg gcgaatacag cgtcgatggc ctgacggtca aaacgctgcc
    51841 tggcgtgttt agccgcgacg gtctggatgt cggtagccag ttgctgctct cgacgttaac
    51901 tccgcacacg aaaggtaaag tgctggatgt cggctgtggc gcgggtgtac tttcagttgc
    51961 ctttgcgcgc cattcgccga aaattcgtct caccttgtgc gatgtctctg ccccggcggt
    52021 agaagccagc cgcgcaacac ttgcagccaa cggtgttgaa ggtgaagtct ttgccagcaa
    52081 cgtcttttcc gaggtgaaag gttgttttga tatgatcatc tccaacccgc cgttccacga
    52141 tgggatgcaa accagcctgg atgcggcgca aacgctgatt cgtggcgcgg tgcgtcatct
    52201 taatagcggc ggcgagctgc gaattgtagc gaacgccttc ctgccttacc cggacgtgct
    52261 ggatgagaca tttggcttcc atgaagttat cacgcaaacc gggcgcttca aggtatatcg
    52321 cgccattatg acccgccagg cgaagaaagg ttgagtatct gatatctgtc gccgcatacc
    52381 gtgtcggatg cggcgtgaac gccttatccg acctacaaaa cctgcacgtt agcccttcgt
    52441 aggccagata agacgcgcca gcgtcgcatc tggcatctgc ccccgcatgc ctaccaaacc
    52501 tgcacgctag tttcctgatg gacatttttc cagcaattac acatcaggcg ataattaact
    52561 attgacgaaa agctgaaaac cactagaatg cgcctccgtg gtagcaattc tttttaagaa
    52621 ttgatggtat gcgaaggtgg cggaattggt agacgcgcta gcttcaggtg ttagtgtcct
    52681 tacggacgtg ggggttcaag tccccccctt cgcaccaaaa accacgttga tattgctcgc
    52741 actgggcgaa ggtggcggaa ttggtagacg cgctagcttc aggtgttagt gttcttacgg
    52801 acgtgggggt tcaagtcccc cccctcgcac caaacgaggc gatatcaaaa aaagtaagat
    52861 gactgtgcga aggtggcgga attggtagac gcgctagctt caggtgttag tgtccttacg
    52921 gacgtggggg ttcaagtccc ccccctcgca ccaattatct ttacttcctt tcttgtttct
    52981 tccctgtttt gtcatccctg attcaaattc attatcatca gcatgatgcc agcaataagc
    53041 gccaggcacg atggtagcaa aatgtaatgc cgcaactgtt tgtgatacag catcaccggc
    53101 gtcaatacta atcccatcag aatgacaatc cgccactgat ccggcgataa atccgcatac
    53161 atcagccctg caatcagcaa tcccggcagc aacactcccc agccactgcc cagcgtacgt
    53221 tgcaacatgg tttcatctct ttcattgata atgaaaacca atatcatatg ataattttta
    53281 tcatttgcaa gccagagaaa tcccttgcta tcgagtaaac ctatcgatag gattagcaat
    53341 cattatcatt tagattacta tcacgattat ggcctatcgt tccgcaccgc tctatgaaga
    53401 tgtcatctgg cgaacgcatc tccagccgca ggatgctggg cttgcacaag ccgtacgggc
    53461 gatgatcgcc aaacatcgtg aacatttgct ggagtttatc cgcctggatg aacccgctcc
    53521 gcttaacgcc atgacgctgg cgcaatggtc atcaccgaat gcgctaagtt ctctactggc
    53581 ggtctattcc gatcatatct atcgcaacca accaacgatg atccgcgaga acaaaccgct
    53641 gatctcacta tgggcacaat ggtatatcgg cctgatggtg ccaccattaa tgctggcgct
    53701 actgacgcag gaaaaagcat tagatgtgtc gccggaacat ttccatgctg agtttcacga
    53761 aaccggacgc gtcgcctgtt tctgggtcga tgtgtgtgaa gataaaaacg caacaccaca
    53821 ttcgccgcag cagcgaatgg aaacgttaat cagccaggcg ctggttccgg ttgtgcaagc
    53881 actagaagcg accggagaga tcaacggcaa acttatctgg agtaataccg gttatttgat
    53941 caactggtat ctcactgaga tgaagcaact gctcggcgag gctactgttg aatcgctgcg
    54001 ccatgccctc ttttttgaga aaacgctcac gaacggtgaa gataatccac tctggcgtac
    54061 cgtggtactg cgcgacggcc tgctggtgcg ccgcacttgc tgccagcgtt atcgcttacc
    54121 ggatgtgcag caatgtggct attgtacgct gaaatgaaag cactgccggg gaagtaaacc
    54181 cggcatcatg cggattaata gggatgcaac acattacttg cttcgcaatg ccgcatacat
    54241 aacagaatat ctgcggcctc caacaaccct gcatcagaac tgacgcccag tttcgacatc
    54301 acattaaatt tatgcgctcg gatcgttttg atattacgtt taagctgctc ggcaatttgt
    54361 gtcattgagt agccacgcga cataaagcgc aatatttctc tctccgtcgg gcttaacgtc
    54421 cggctttggt taatgtacca ctgattgttc agtcggtcgg tcgcctgacg tacaccattt
    54481 aatgacaaaa agaattcctg atgaaaaatc tccagcgttg acgctttact taatacaccg
    54541 tccagcggtg atggcgacaa tgaaccaatc agccgagctt caatatcatc atccacaata
    54601 actaaacgcc gggtacgcgg aaacttaatc gccagctcag tcaggcaaga taatccctct
    54661 ctccgctcac ttctcatggc agaaaaagaa aaaatgaccg ccgcaaacga gatccgcgcc
    54721 atggcatcct gaaatgactc ctgactggta taggtatgga gcttataagg gttgcccgca
    54781 aactttctga ataaactctc aataccgata ctactcatga cgcatttttc tatgagtgcg
    54841 acatttctct tcttaattcg gctgt
//
LOCUS       DAJVQH010000013        52689 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJVQH010000013 DAJVQH010000000
VERSION     DAJVQH010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.013"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(11..322)
                     /locus_tag="OQM87_000888"
     CDS             complement(11..322)
                     /locus_tag="OQM87_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCS1507128.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OQM87_000889"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OQM87_000889"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OQM87_000890"
     CDS             701..1054
                     /locus_tag="OQM87_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCS1507129.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OQM87_000891"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OQM87_000891"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCS1507130.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLHQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="OQM87_000892"
     CDS             complement(2810..3280)
                     /locus_tag="OQM87_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCS1507131.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OQM87_000893"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OQM87_000893"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCS1507132.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OQM87_000894"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OQM87_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1507133.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OQM87_000895"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OQM87_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCS1507134.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="OQM87_000896"
     CDS             complement(5283..8432)
                     /locus_tag="OQM87_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1507135.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OQM87_000897"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OQM87_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCS1507136.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="OQM87_000898"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="OQM87_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCS1507137.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="OQM87_000899"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="OQM87_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCS1507138.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OQM87_000900"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OQM87_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCS1507139.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OQM87_000901"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OQM87_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCS1507140.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="OQM87_000902"
     CDS             14911..15288
                     /locus_tag="OQM87_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCS1507141.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="OQM87_000903"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="OQM87_000903"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCS1507142.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="OQM87_000904"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="OQM87_000904"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCS1507143.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="OQM87_000904"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="OQM87_000905"
     CDS             18099..18428
                     /locus_tag="OQM87_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCS1507144.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="OQM87_000906"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="OQM87_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCS1507145.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="OQM87_000907"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="OQM87_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCS1507146.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="OQM87_000908"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="OQM87_000908"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCS1507147.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="OQM87_000909"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="OQM87_000909"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCS1507148.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23893)
                     /gene="aes"
                     /locus_tag="OQM87_000910"
                     /pseudo
     CDS             complement(22933..23893)
                     /gene="aes"
                     /locus_tag="OQM87_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24045..25349
                     /gene="gsk"
                     /locus_tag="OQM87_000911"
     CDS             24045..25349
                     /gene="gsk"
                     /locus_tag="OQM87_000911"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCS1507149.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25482..27158)
                     /locus_tag="OQM87_000912"
     CDS             complement(25482..27158)
                     /locus_tag="OQM87_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCS1507150.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGKIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="OQM87_000913"
     CDS             complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="OQM87_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCS1507151.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSQGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28834..30486
                     /gene="ushA"
                     /locus_tag="OQM87_000914"
     CDS             28834..30486
                     /gene="ushA"
                     /locus_tag="OQM87_000914"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1507152.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="OQM87_000915"
     CDS             complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="OQM87_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCS1507153.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31206..32000)
                     /locus_tag="OQM87_000916"
     CDS             complement(31206..32000)
                     /locus_tag="OQM87_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCS1507154.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32138..32479
                     /locus_tag="OQM87_000917"
     CDS             32138..32479
                     /locus_tag="OQM87_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1507155.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32694..35198)
                     /gene="copA"
                     /locus_tag="OQM87_000918"
     CDS             complement(32694..35198)
                     /gene="copA"
                     /locus_tag="OQM87_000918"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCS1507156.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35460..36392
                     /gene="glsA"
                     /locus_tag="OQM87_000919"
     CDS             35460..36392
                     /gene="glsA"
                     /locus_tag="OQM87_000919"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCS1507157.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36395..37687
                     /locus_tag="OQM87_000920"
     CDS             36395..37687
                     /locus_tag="OQM87_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1507158.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37812..38219
                     /gene="cueR"
                     /locus_tag="OQM87_000921"
     CDS             37812..38219
                     /gene="cueR"
                     /locus_tag="OQM87_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCS1507159.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38220..38678)
                     /locus_tag="OQM87_000922"
     CDS             complement(38220..38678)
                     /locus_tag="OQM87_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCS1507160.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38675..39592)
                     /locus_tag="OQM87_000923"
     CDS             complement(38675..39592)
                     /locus_tag="OQM87_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1507161.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39738..40415
                     /gene="fetA"
                     /locus_tag="OQM87_000924"
     CDS             39738..40415
                     /gene="fetA"
                     /locus_tag="OQM87_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCS1507162.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40402..41181
                     /gene="fetB"
                     /locus_tag="OQM87_000925"
     CDS             40402..41181
                     /gene="fetB"
                     /locus_tag="OQM87_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCS1507163.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="OQM87_000926"
     CDS             complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="OQM87_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCS1507164.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="OQM87_000927"
     CDS             complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="OQM87_000927"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1507165.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="OQM87_000928"
                     /pseudo
     CDS             complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="OQM87_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43551..44237
                     /locus_tag="OQM87_000929"
     CDS             43551..44237
                     /locus_tag="OQM87_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1507166.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44234..46648
                     /locus_tag="OQM87_000930"
     CDS             44234..46648
                     /locus_tag="OQM87_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1507167.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OQM87_000931"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OQM87_000931"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCS1507168.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OQM87_000932"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OQM87_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCS1507169.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="OQM87_000933"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="OQM87_000933"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCS1507170.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="OQM87_000934"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="OQM87_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCS1507171.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..>52689
                     /gene="gcl"
                     /locus_tag="OQM87_000935"
     CDS             51144..>52689
                     /gene="gcl"
                     /locus_tag="OQM87_000935"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCS1507172.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALNSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVA"
BASE COUNT        12685 a        13608 c        13875 g        12521 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca acgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggtgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgac tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccggg aagggcagga tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctggc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aatcagcgag aatttgctgg cgtaatgtgt gctcttccgc tagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagct ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcat tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc cagccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaaccg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gtttttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttaaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagccgg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgtgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgcagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgctactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcatca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caatatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacta ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg cggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaagc
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactct gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacgg ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgttcgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggcgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggcgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca ggcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgac atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaacc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagccg gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctc atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc gccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactcctccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatca ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgccttcc atgcaaataa aatagcgtcg
    23641 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    23701 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    23761 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccggct
    23821 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt
    23881 tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    23941 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    24001 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta
    24061 aacgtaaatc caaaaattac ttccctgtaa atgcacgcga tccgctgctt cagcaattcc
    24121 agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg ctggtcgata
    24181 ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac
    24241 tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag aaaaacctga
    24301 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac tcggtgctcg
    24361 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagttatg
    24421 cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta caaggcgtgg
    24481 atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    24541 tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat gtgattgccg
    24601 gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt gaacccatgc
    24661 cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga
    24721 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    24781 accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    24841 atccgttgtt ggcatctgac aaggcgctgg actgggtaga tctggtgctg tgcaccgccg
    24901 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    24961 atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc
    25021 gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg tacatgggcg
    25081 ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc
    25141 atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat
    25201 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    25261 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    25321 gcctggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg
    25381 ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg cgtcttatca
    25441 tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca
    25501 cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc
    25561 actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg
    25621 gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc acccgcttca
    25681 taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc cagttgcata
    25741 atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg cagctcatca
    25801 acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag caatttctcc
    25861 cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca aatatccact
    25921 gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc
    25981 ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc
    26041 gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    26101 cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc
    26161 gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga tttaccaaac
    26221 agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca
    26281 aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcaacgta
    26341 tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc aaagaacgca
    26401 ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc
    26461 gccagcactg atagggtaaa cagttcgcga gaaccggttg ccgcgctgcg tgccataatc
    26521 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    26581 atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg ttccatcatt
    26641 cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc cacaatcaac
    26701 caaccgatgg cgattttccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    26761 agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc ggtcattaac
    26821 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc tatctgggcg
    26881 atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga aaaatgcaaa
    26941 ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc aagcttggta
    27001 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    27061 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    27121 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    27181 tattgtccat ttttggccgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    27241 tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    27301 gcacggatcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac
    27361 gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc
    27421 aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg
    27481 atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc catgccgaaa
    27541 gcaaaaccga aaaagagtcc agaaatcata ccgatacgtc ccggaagtag ctcctgagcg
    27601 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    27661 cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    27721 gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta
    27781 cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    27841 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    27901 ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt
    27961 ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    28021 cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg
    28081 ttgcctttgc cataaggcgc gataatcacc gccgccagca aggggccctg ggaactgcca
    28141 aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata
    28201 cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc
    28261 gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta
    28321 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc
    28381 actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    28441 aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc
    28501 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc
    28561 ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat tgccatagga
    28621 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    28681 aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    28741 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt
    28801 ttaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    28861 ttagcgctgt taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat
    28921 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    28981 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    29041 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    29101 cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat
    29161 gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag
    29221 gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag
    29281 cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg
    29341 ctgacaaccg atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt
    29401 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    29461 gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac
    29521 gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    29581 ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    29641 gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat
    29701 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    29761 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    29821 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    29881 tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa aaccaatggt
    29941 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt
    30001 ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt
    30061 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    30121 ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc
    30181 gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg
    30241 gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa
    30301 acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt
    30361 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    30421 atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    30481 cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat
    30541 atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag
    30601 tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    30661 cgtgggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga
    30721 acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    30781 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    30841 accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    30901 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    30961 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    31021 aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc
    31081 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    31141 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    31201 tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc
    31261 gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc
    31321 tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta
    31381 ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta
    31441 tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac
    31501 atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    31561 gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    31621 gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag tgagggagaa
    31681 atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct
    31741 tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    31801 tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    31861 atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt gccggggagg
    31921 gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    31981 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga
    32041 gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct
    32101 tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc
    32161 gactacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa
    32221 tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga tcaataacaa
    32281 tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga
    32341 ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac
    32401 ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg
    32461 tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt
    32521 cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg tcttatcagg
    32581 cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc
    32641 atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc
    32701 ttcggtttaa accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc
    32761 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    32821 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    32881 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    32941 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    33001 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    33061 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    33121 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    33181 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    33241 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    33301 acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc tttagtatcg
    33361 acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc ttctgcttca
    33421 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatcg
    33481 cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc cagcgccgcc
    33541 gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc gacaacctgc
    33601 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    33661 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    33721 gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat aatcagcacc
    33781 gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt
    33841 gccgcactga caagcgcaat aaccactact accggcacaa atacggctga gattttatcc
    33901 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatcctt
    33961 gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc
    34021 actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    34081 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    34141 tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg cacgcttttt
    34201 tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagt
    34261 gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt gatcagaccg
    34321 ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa ccactgcggc
    34381 cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc caccagcgta
    34441 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg
    34501 gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct gcggttgtcg
    34561 gcggtgacca tcatgttatc gccgatcatc ccccagacca tcaccgggat acccaccgcc
    34621 agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg
    34681 cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc
    34741 gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg
    34801 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    34861 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    34921 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    34981 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    35041 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgct
    35101 tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag
    35161 gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt taagacagtt
    35221 ttgactggct gtgataaagg ttaaaccttc cagaaagggg aaggtcaaga aattaataaa
    35281 ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac
    35341 atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg
    35401 aaaatcggca caggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    35461 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    35521 ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg
    35581 cggcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc
    35641 gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    35701 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    35761 ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcta
    35821 ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag cgaattttac
    35881 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg
    35941 aacaaacaac caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc
    36001 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca
    36061 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga
    36121 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    36181 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    36241 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    36301 caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    36361 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    36421 taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc
    36481 ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctggggcgc
    36541 ctttgctttt ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc
    36601 gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt
    36661 cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca tcgccatggt
    36721 tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca
    36781 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    36841 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    36901 attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc
    36961 ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt
    37021 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    37081 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct
    37141 tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc
    37201 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    37261 ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    37321 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    37381 catcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag actcactcgc
    37441 cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg tggcgatccg
    37501 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    37561 gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg cgttgatatg
    37621 gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc gtaataaaac
    37681 cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    37741 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg
    37801 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca
    37861 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    37921 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    37981 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    38041 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    38101 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    38161 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat
    38221 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatac
    38281 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    38341 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    38401 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    38461 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc
    38521 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac
    38581 aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc gagactgagc
    38641 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    38701 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    38761 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    38821 tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca
    38881 ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg
    38941 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
    39001 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
    39061 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    39121 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    39181 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    39241 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcatagg
    39301 cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    39361 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    39421 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    39481 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    39541 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
    39601 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
    39661 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
    39721 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
    39781 atctggcggg tgatacgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
    39841 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
    39901 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
    39961 aaccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg ctgtttggcg
    40021 atacggtata cgataatctg atctttccct ggcagatccg tgaccagcag cctgacccgg
    40081 ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg acgaagaata
    40141 tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac ctacaattta
    40201 tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt aataaacata
    40261 acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
    40321 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
    40381 gagaaatgca ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg
    40441 gcattaatgc tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag
    40501 aaagatattc tctggagcgt cgggcgagcg ataattcagc tgattattgt cggctatgtg
    40561 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
    40621 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
    40681 tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca
    40741 gggtcgattg aatttatccc gatgcaggtg atccctgtcg ccgggatgat tgccggtaac
    40801 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
    40861 caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc agcgatattg
    40921 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
    40981 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
    41041 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
    41101 attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
    41161 actcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
    41221 ttcatgtgcc gaacaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
    41281 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
    41341 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
    41401 tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct
    41461 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcttatcagc cgcctgcttc
    41521 agcagttcga tttgcgctac cagcccctgg tagcgggtgt cctgatcctg caacggaatg
    41581 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
    41641 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg
    41701 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
    41761 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
    41821 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
    41881 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
    41941 ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg ctggctacgt
    42001 tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg
    42061 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
    42121 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
    42181 atcacgcgcc caggcagcag gcgctcgagc accattactg cccaggtcac cagcgttacc
    42241 ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc gaccaccgct
    42301 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
    42361 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
    42421 ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc cagcgcatat
    42481 ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac cgatgatgtc
    42541 atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg
    42601 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
    42661 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
    42721 agggcgatca cctcgtctgc tgcgcgatta acactttctg gtgaatccag atcgatcaac
    42781 acgccggtaa atcccatgtt gttcatacgc tcaacatcat tcggttttcg gcaacctgcc
    42841 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
    42901 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
    42961 tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg
    43021 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
    43081 ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
    43141 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
    43201 tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
    43261 ctgtggctga aaaccacgca aaccatcatt gcgcccagtt caaccagcac ccaacgcggc
    43321 tgatgctgtt tgagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg
    43381 atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag
    43441 gccgcgctgg cagacattcg atatccggcg ctcaggctat cacccagaat caataacgtg
    43501 tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa atgccagcgg
    43561 aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct
    43621 ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg
    43681 agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca
    43741 gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa
    43801 agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta
    43861 acgcgctgga aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc
    43921 gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc
    43981 cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc
    44041 gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata
    44101 aaattgccga cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga
    44161 cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt
    44221 tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt
    44281 aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag
    44341 cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc
    44401 gttgcgcagt tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct
    44461 gaaagtcggc aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct
    44521 ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc
    44581 ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct
    44641 taacctgaaa acggacgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga
    44701 agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat
    44761 gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg
    44821 ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt tgttacctca
    44881 gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc
    44941 gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt
    45001 ggcagcggtg gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat
    45061 cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt
    45121 gatggtgctg acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt
    45181 gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc
    45241 gtggctgtgg gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata
    45301 ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tcgcgaacgt
    45361 ctggccgctg aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg
    45421 attaatgggt ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt
    45481 gctgtgcggt gtgctgggct ggatgctgct gaatgtactt cgccgcatga cgctgaaatc
    45541 gctgcctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca
    45601 gctttcggca ttttcgctct cctttatgct gctggcactg ctgctggtgc tgcgtggcga
    45661 tctgctcgac cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa
    45721 catcgccaca gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc
    45781 ggaatcattt tatccggtag tgcgggcgcg gctgacggcg attaacgata agccgacaga
    45841 aggtaatgaa gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc
    45901 cgatcataat ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga
    45961 agaggggctg gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga
    46021 tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg
    46081 gcctaatttc tattttattt tccctgaagg ggcattagac gggcaaccgc agagctggct
    46141 taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc
    46201 gaccattagc ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg tgctggagca
    46261 ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt
    46321 gctggcacag gtgcaggtgg gaatgcgcca gcgtcatcag gagctggtgg tatggcgcac
    46381 actcggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgagttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tgtaactatg attttcttca tgtgctagat gaggactgcc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aacaaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggcggc gcgttcatcg ttattcatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtgggttgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgccgctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcg
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggaccaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gcgttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt taaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctcaattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgatgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcactg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgg
//
LOCUS       DAJVQH010000014        50199 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJVQH010000014 DAJVQH010000000
VERSION     DAJVQH010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.014"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..373
                     /locus_tag="OQM87_000936"
     CDS             <1..373
                     /locus_tag="OQM87_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1507173.1"
                     /translation="IAGYYMKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQIL
                     NAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHAL
                     FWLAIGMLGIAMICLLFIKDI"
     gene            484..1407
                     /locus_tag="OQM87_000937"
     CDS             484..1407
                     /locus_tag="OQM87_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1507174.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="OQM87_000938"
     CDS             complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="OQM87_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCS1507175.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2451..2744
                     /locus_tag="OQM87_000939"
     CDS             2451..2744
                     /locus_tag="OQM87_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507176.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="OQM87_000940"
     CDS             complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="OQM87_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCS1507177.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4186..4638)
                     /locus_tag="OQM87_000941"
     CDS             complement(4186..4638)
                     /locus_tag="OQM87_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCS1507178.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4691..6025)
                     /gene="adeQ"
                     /locus_tag="OQM87_000942"
                     /gene_synonym="yicO"
     CDS             complement(4691..6025)
                     /gene="adeQ"
                     /locus_tag="OQM87_000942"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCS1507179.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6200..7966
                     /gene="adeD"
                     /locus_tag="OQM87_000943"
     CDS             6200..7966
                     /gene="adeD"
                     /locus_tag="OQM87_000943"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCS1507180.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPAVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="OQM87_000944"
     CDS             complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="OQM87_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCS1507181.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="OQM87_000945"
     CDS             complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="OQM87_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCS1507182.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="OQM87_000946"
     CDS             complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="OQM87_000946"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCS1507183.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="OQM87_000947"
     CDS             complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="OQM87_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCS1507184.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13037..13327)
                     /gene="ilvN"
                     /locus_tag="OQM87_000948"
     CDS             complement(13037..13327)
                     /gene="ilvN"
                     /locus_tag="OQM87_000948"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1507185.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="OQM87_000949"
     CDS             complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="OQM87_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCS1507186.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="OQM87_000950"
     CDS             complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="OQM87_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCS1507187.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15788..15877
                     /gene="tisB"
                     /locus_tag="OQM87_000951"
     CDS             15788..15877
                     /gene="tisB"
                     /locus_tag="OQM87_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCS1507188.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16157..17341
                     /gene="emrD"
                     /locus_tag="OQM87_000952"
     CDS             16157..17341
                     /gene="emrD"
                     /locus_tag="OQM87_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCS1507189.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17349..17846)
                     /locus_tag="OQM87_000953"
     CDS             complement(17349..17846)
                     /locus_tag="OQM87_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCS1507190.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17843..18205)
                     /locus_tag="OQM87_000954"
     CDS             complement(17843..18205)
                     /locus_tag="OQM87_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCS1507191.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18195..18542)
                     /locus_tag="OQM87_000955"
     CDS             complement(18195..18542)
                     /locus_tag="OQM87_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCS1507192.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLTWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18651..19064
                     /locus_tag="OQM87_000956"
     CDS             18651..19064
                     /locus_tag="OQM87_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507193.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19111..20494)
                     /locus_tag="OQM87_000957"
                     /pseudo
     CDS             complement(19111..20494)
                     /locus_tag="OQM87_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(20529..22244)
                     /locus_tag="OQM87_000958"
                     /pseudo
     CDS             complement(20529..22244)
                     /locus_tag="OQM87_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22411..23304
                     /locus_tag="OQM87_000959"
     CDS             22411..23304
                     /locus_tag="OQM87_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1507194.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23301..24115)
                     /locus_tag="OQM87_000960"
                     /pseudo
     CDS             complement(23301..24115)
                     /locus_tag="OQM87_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24115..25731)
                     /locus_tag="OQM87_000961"
                     /pseudo
     CDS             complement(24115..25731)
                     /locus_tag="OQM87_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            26026..26700
                     /locus_tag="OQM87_000962"
     CDS             26026..26700
                     /locus_tag="OQM87_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1507195.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(26739..28400)
                     /locus_tag="OQM87_000963"
     CDS             complement(26739..28400)
                     /locus_tag="OQM87_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCS1507196.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(28596..29024)
                     /gene="ibpB"
                     /locus_tag="OQM87_000964"
     CDS             complement(28596..29024)
                     /gene="ibpB"
                     /locus_tag="OQM87_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCS1507197.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(29136..29549)
                     /gene="ibpA"
                     /locus_tag="OQM87_000965"
     CDS             complement(29136..29549)
                     /gene="ibpA"
                     /locus_tag="OQM87_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCS1507198.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(29543..29615)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            29854..30186
                     /locus_tag="OQM87_000966"
     CDS             29854..30186
                     /locus_tag="OQM87_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1507199.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGSKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(30188..31402)
                     /locus_tag="OQM87_000967"
     CDS             complement(30188..31402)
                     /locus_tag="OQM87_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCS1507200.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            31503..32521
                     /locus_tag="OQM87_000968"
                     /pseudo
     CDS             31503..32521
                     /locus_tag="OQM87_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(32482..33819)
                     /locus_tag="OQM87_000969"
     CDS             complement(32482..33819)
                     /locus_tag="OQM87_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1507201.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(33894..35042)
                     /gene="dgoD"
                     /locus_tag="OQM87_000970"
                     /pseudo
     CDS             complement(33894..35042)
                     /gene="dgoD"
                     /locus_tag="OQM87_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(35039..35656)
                     /gene="dgoA"
                     /locus_tag="OQM87_000971"
     CDS             complement(35039..35656)
                     /gene="dgoA"
                     /locus_tag="OQM87_000971"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCS1507202.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKVVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(35640..36518)
                     /gene="dgoK"
                     /locus_tag="OQM87_000972"
     CDS             complement(35640..36518)
                     /gene="dgoK"
                     /locus_tag="OQM87_000972"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCS1507203.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLLR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(36515..37204)
                     /gene="dgoR"
                     /locus_tag="OQM87_000973"
     CDS             complement(36515..37204)
                     /gene="dgoR"
                     /locus_tag="OQM87_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCS1507204.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            37482..38138
                     /locus_tag="OQM87_000974"
     CDS             37482..38138
                     /locus_tag="OQM87_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507205.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(38184..38996)
                     /gene="yidA"
                     /locus_tag="OQM87_000975"
     CDS             complement(38184..38996)
                     /gene="yidA"
                     /locus_tag="OQM87_000975"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCS1507206.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(39110..39508)
                     /locus_tag="OQM87_000976"
     CDS             complement(39110..39508)
                     /locus_tag="OQM87_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCS1507207.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(39748..42162)
                     /gene="gyrB"
                     /locus_tag="OQM87_000977"
     CDS             complement(39748..42162)
                     /gene="gyrB"
                     /locus_tag="OQM87_000977"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCS1507208.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(42191..43264)
                     /gene="recF"
                     /locus_tag="OQM87_000978"
     CDS             complement(42191..43264)
                     /gene="recF"
                     /locus_tag="OQM87_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCS1507209.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPRFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(43264..44364)
                     /gene="dnaN"
                     /locus_tag="OQM87_000979"
     CDS             complement(43264..44364)
                     /gene="dnaN"
                     /locus_tag="OQM87_000979"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCS1507210.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(44369..45772)
                     /gene="dnaA"
                     /locus_tag="OQM87_000980"
     CDS             complement(44369..45772)
                     /gene="dnaA"
                     /locus_tag="OQM87_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCS1507211.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            46069..46149
                     /locus_tag="OQM87_000981"
     CDS             46069..46149
                     /locus_tag="OQM87_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507212.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            46379..46519
                     /gene="rpmH"
                     /locus_tag="OQM87_000982"
     CDS             46379..46519
                     /gene="rpmH"
                     /locus_tag="OQM87_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCS1507213.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            46536..46895
                     /gene="rnpA"
                     /locus_tag="OQM87_000983"
     CDS             46536..46895
                     /gene="rnpA"
                     /locus_tag="OQM87_000983"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCS1507214.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCLLARGS"
     gene            46859..47116
                     /gene="yidD"
                     /locus_tag="OQM87_000984"
     CDS             46859..47116
                     /gene="yidD"
                     /locus_tag="OQM87_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCS1507215.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            47119..48765
                     /gene="yidC"
                     /locus_tag="OQM87_000985"
     CDS             47119..48765
                     /gene="yidC"
                     /locus_tag="OQM87_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCS1507216.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            48871..>50199
                     /gene="mnmE"
                     /locus_tag="OQM87_000986"
     CDS             48871..>50199
                     /gene="mnmE"
                     /locus_tag="OQM87_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCS1507217.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLL"
BASE COUNT        12395 a        13502 c        12662 g        11640 t
ORIGIN      
        1 aatcgcaggc tattacatga aacgtattgg caagcgacta ttaatgctca ttgctatcgt
       61 gagtggtatg tgtttttacg ccagcgtact catggcgacg actccggcgg ttgagctgga
      121 attgcaaatt ctaaatgcca tcttccttgg tattctctgt ggtatcggca tgctttattt
      181 tcaggacttg atgcctgaaa aaataggctc tgcgacaacg ttatatgcaa atacttcacg
      241 cgtcggctgg attatcgccg gctctgttga cggaattatg gttgaaatct ggagctacca
      301 tgcgttgttc tggctggcga tagggatgtt gggtattgcg atgatttgcc tgctgtttat
      361 taaagatatt tagctcacca gtctgggcta agaaaaatcg gcgtggcgca aacagagcca
      421 tcctcgccat ttttgctatc atgcctgcat acataaacga caaaacagta tgcagaggga
      481 aaaatgggtt ccaccagaaa ggggatgctg aacgttctga ttgccgccgt gttgtgggga
      541 agttcagggg tctgtgcgca atacatcatg gagcaaagcc agatgtcgtc gcagtttttg
      601 actatgacgc gtttgatatt cgccggtttg attctgctga cgctgtcatt tgttcatggc
      661 gataaaatct tttctattat taacaatcac aaagatgcca ttagtctgct gattttttcc
      721 gtggttggcg cgctaaccgt acagctcact tttttgctaa ccatcgaaaa atcgaacgca
      781 gccacggcaa cggtgctgca attcctctca ccgacgatta tcgtcgcctg gttctcactg
      841 gtacgtaaat cgcgcccggg cattctggtt ttctgcgcta ttttgacatc gctggtcggg
      901 acttttttat tggtgacaca cggtaatccg acgtcattat cgatctctcc tgccgcgtta
      961 ttctggggca ttgcctcggc atttgctgct gcattctata ccacctatcc ctcaacgctg
     1021 attgcccgct atggcacgtt gcctgtcgtc ggctggagta tgctgatcgg cggtctgatt
     1081 ctattgcctt tttatgccag acaagggaca aactttgtcg ttaacggcag tttgattttg
     1141 gcgttttttt atttgatcgt cattggtacg tccctgacat ttagtctgta cctgaaagga
     1201 gcacaattaa ttggcggtcc aaaagccagc attttgagct gtgcagaacc attaagtagc
     1261 gcgctactct ctttgctgtt gctggggatc acgttcacat taccggactg gctgggaacg
     1321 ctgctgattc tgtcatcggt aattttgatt tcaatggatt cccgtcgccg cgccagaaaa
     1381 ataaatcgtc cggcgcggga tgagtgagaa ttaccagcca ggcaccgcgc caccgttaaa
     1441 gatggtttct gccgctttag cgacttcgga tgattgataa gattgcagaa attccttcac
     1501 gttttctgca ttcttattat cttcccgtgc caccaaaata ttcacatacg gcgaattttt
     1561 atcttcaata aatacactgt cgtgcaccgg agaaagcccg gtctgctgaa tgtaagtggt
     1621 gctgataatc gccacatcaa ctttgggatc atccagtacg cgcgggagct gcgccccctc
     1681 gagttccata atctgcaaat gacgaggatt atcggttata tccagcgcgg taggtaataa
     1741 cccttttccc tctttcagag taattagctt ctctttttgc aacagtaaaa gcgcacgacc
     1801 aaggttggtc gggtcgttgg ggatcgccac tgtcgcgcct tcttttattt gcgccacggt
     1861 tttgattttt ttggaataac ccgccatggg gaagacaaac gtatttccca ccgctaccag
     1921 tttataacca tgcgcctgat tatcttgttc aagaaagggt cgatgctgga agacgttcgc
     1981 atcaagttcg ccatgatttg tcgcatcgtt cggtagcaac gaaccgctaa atcctaccag
     2041 ttgaacatcg aggccatatt tctcttttgc caccttttta gcgacttccg cgacatcttg
     2101 ttctgcgcca tttataacgc caactttaat gtgttttgca tcgctgctac tctggtcgca
     2161 acctgccagc agaattccgg ccagcaataa tgcggcccct gtccgtagat gatgtgttgt
     2221 cagtttcatt tttttatcct tttattaatg attacctgac tatagcggta aggtcgctgc
     2281 ggtttaaaaa acgaaacgct atcgataaga ataaaaagga ataaaagtgg aatataaagt
     2341 ctggcacttc ttactgacca cgcaagcgcg tttcgtacag catgatgaga gcgatgaaag
     2401 caaactacac ttatgcttca ttcgatacac atttgtaaaa ggttgaatcc atgaaaccaa
     2461 cgacactact gcttattttc acttttttcg ctatgccagg catcgtatac gccgagtcgc
     2521 cttttagctc actgcagtcc gcaaaagaga aaaccacggt attacaagat ttacgtaaaa
     2581 tttgcacgcc acaggcgtca ttatcagatg aagcgtggga aaagttaatg ttgtcagatg
     2641 aaaacaataa acaacatata cgcgaggcca tcgtggcgat ggaacgtaat aatcagagca
     2701 attattggga agcgttgggt aaggtagaat gcccggatat gtaagaacag caacaactac
     2761 tgaccaacgc ttctttacct gatatcagga tttcttcatt ttcacctttg cagtaaccaa
     2821 caatgcagtc agcaacatca atgtgccgga caacatcaac ggcgaagtca gaccaatatt
     2881 atccagcgca taaccgccga ttgccgcgcc acaggtatta gcaagctgaa taaccgccac
     2941 ctgaatagac ccggcttttt ctgcctgatc ggccagcgag cgggtgatcc acgttgacca
     3001 gccgacggga accaatgcaa aagttagccc ccagataatc gccacgccgg tagcaacgat
     3061 tttatcgctt ccccacaacg tcagtaccaa cgcactcaca gccagtatta acggcgcgcc
     3121 tgctaaggcc agttttaccg aacgtttaag aatgaacgac gaaagcgacg taccaataaa
     3181 gctggcgata ccaaaactca acagcaccag cgttaagcca tccacgccga atcccgccag
     3241 gttcatatac accgggcgaa tatacgtgaa gaaagcaaac tgcccggcga aagacatgaa
     3301 gatggcgatc atccctgcca tcacacccgg acgttgtaat aagcggaaag tattttgttt
     3361 ctgatgcgag ggttcgcctg gcagtgaagg caatgatttg ataatccaga aaatacacag
     3421 cacgcccatc accgccgccg cattaaagac attgcgccaa ccgataagct cgcctaaaaa
     3481 actgcccaac ggcgcggcaa tcaccagcgc aatagaaacc gcgccgaaga tcaccgacag
     3541 cgccttcggc accgtacgcg gcggtaccag acgcatggtc agcgacgccg acatcgccca
     3601 gaacccgccc agcgccagcc ccagacaggc acgaccgatt aaaagcaaac tgaatgagtt
     3661 agcaaaggaa accagcaagc aggagagcgt cagcaaaacg gcaaacaaaa taacaacgta
     3721 gcggcggtca gtggcctgaa ttgtctgggt aataaacaaa ctggcaaaca ttgccacaaa
     3781 ggcggtcacg gtcaccgatt gcccggcaac cccttccgaa atgcctaaat cctgggccat
     3841 tggcgtcaac aaactgacgg gcaaaaactc aacgataatc agacaggcga cacaaaacgc
     3901 caccgagaaa acggctgacc agttcggtcg ggtgatggca tccgcgccgc ggttttcggc
     3961 aataaattca ctcatggtgt tacccgtggc atgtttcagg ggaaaagccg accactttaa
     4021 cattgaaatg tgtgacgcat ttaacgtttt tgcaactttt acggtgttgc ttgagctgga
     4081 gttaagatcg aaacggaaca gggcaaagat taccggatgc ggcgtgaacg tcttatccac
     4141 ccgacaggtc aggcgcggtc tgtaggctga taagacgaaa cggtttcaat gcaaccagtg
     4201 aacgccctcg gcaggctgaa agaacgcgtt atagcgcatc tgaaaagcat taatatcctg
     4261 catttcaggt tcggtttcac gcagaaaacc cagcgccagc ctgacctgag catcggtaat
     4321 cggcgcggaa aggcgggctt tttgtaaaat ccggtgccat tgcagcaggt tctgttcgct
     4381 atcatcgaca atgacaattt gccagataag caacacctgc gcggcgtttt gcaggtgcga
     4441 ctcatccggg cgatgcaaat aacgtaaaaa ttcgtcaccg gctgactttc ccatttgatc
     4501 gaccatcgac tccacgggta cgacatcgat attcagccga tcgctgagac ggcgaatcgc
     4561 ttttctggcc ccggatgtca gcacccgaaa accaaccaca aacactaccg ccagcgtggc
     4621 gagcattatc cagatcatga gttctcctgc gtaatcagaa ctcatgatat ctggaaaccc
     4681 tcgccagtta ctaatccacc agaataatct tcaatgcgaa cagagctgcg accaccacca
     4741 cacacaggtt cagatcgcgc cagcgcccgg tgcacacttt catgatgcag tacgacataa
     4801 agccgagtgc aatcccttcg gtgatcgaga aagtaaaggg catcatcacc gtggtaataa
     4861 acgcaggcac cgattcggta aaatcatccc agttaacgcg cgccaggctc gaagtcatca
     4921 gcacgccaac aaagattaac gctccagcgg ttgcgtaagg aggaaccatc gccaccagcg
     4981 gtgagaagaa cataactaac aggaacataa cgccgaccac gacagccgtt agccccgtgc
     5041 gaccaccgac tgccacacca gaagtacttt caatataggc agtaacagac gaggtaccga
     5101 taaatgcgcc tgccaccgaa ctgacgctat caacatacag cgccttattc atattgggga
     5161 atttaccgtt gctatctatt aagcccgctt tatcagttac accaattaat gttcctgatg
     5221 aatcaaatag gttgatcagc ataaaggaga aaatgatacc ggcgagttca agtgttaacg
     5281 cgccgctcaa atctacttca ccaatgacgc cgctaatatc aggcggaatg gaatagacgc
     5341 cgctaaaatg aacatcaccg aaaaataatc cacagcaaga cgtcaccaca atagaaacca
     5401 gcaccgcggc atgaaaatga cgtgatgaca acacggttat aataaaaaac cctaaaatac
     5461 ctaacaacac gccgtgagaa cttaaatcgc caatcatcac cagcgtgtct ttattggcga
     5521 caataacacc agtgtttttt aatcccatta aggcaataaa taatccgatt ccactggtga
     5581 taccaatacg taaacttaat ggaatgttgg agatcatcca gtaccggata cgaaacagcg
     5641 tgagcaaaaa tagcccaatt gcgccccaga atatcgcgcc catcccggtc tgccaggaga
     5701 tccccatcgc ccccacgacc acgaacgcaa agaaggcgtt cagccccatt gccggagcca
     5761 gcaccacggg taagttagca aatatcccca tcgcaatact gccgataccg gcaatcaaac
     5821 aggtggtaac aaacaccact ttcgggtcca tttgtgccgc gccgaggatt tgcgggttca
     5881 caaaaacaat gtacaccatg gttaaaaaag tggtcatccc cgcaatcaat tctgtgcgga
     5941 cggtggtccc atgctgaggt agtttaaata atcgcgaaag cgtccctgat tcattactca
     6001 cgtaatcggt attgtcatta ttcatttttt tgtccattgg aggagattta atcccttgct
     6061 tttgtaaaac aaatgacaac aacggttcag tgataattat cacatttcaa ttgcacatta
     6121 atagatattc tttaataatc tcgcgacgtt tctttatgat aaataataat caaattgata
     6181 aaatcaaaat gagaaaaata tgaataattc tattaaccat aaatttcatc acattagccg
     6241 ggctgaatac caggaattgt tagccgtttc ccgtggcgac gctgttgccg attatattat
     6301 tgataatgtc tctattctcg acctgatcaa tgccggagaa atttccggcc caattgtgat
     6361 taaaggacgt tacattgccg gtgttggcgc agaatacgct gatactccgg ctttgcagcg
     6421 gattgatgct cgcggcgcaa cggcggtgcc agggtttatt gatgctcacc tgcatattga
     6481 atccagcatg atgacgccgg tcacttttga aaccgctacc ctgccgcgcg gcctgacgac
     6541 cgttatttgc gaccctcatg aaatcgtcaa cgtgatggga gaagccggat tcgcctggtt
     6601 tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtgcc
     6661 cgctctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg
     6721 gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactaccctg ccgtaattaa
     6781 cgggcagaat gcgctgctcg ataaactcga tgcatttcgc tacctgacgc tggacggtca
     6841 ctgtccgggt ttgggtggca aagaacttaa cgcctatatt gctgcgggta ttgaaaactg
     6901 ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggca tgtcgttgat
     6961 gatccgcgaa gggtccgctg cccgcaatct caacgcactg gcaccgttga tcaacgaatt
     7021 taacagcccg caatgcatgc tctgtaccga tgaccgtaac ccgtgggaga tcgcccatga
     7081 agggcatatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt
     7141 ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctcggctt
     7201 actggcaccc ggcaagcagg ccgatatcgt cctgttgagc gatgcgcgta aggtcacggt
     7261 gcagcaggta ctggtgaaag gcgaaccgat tgatgcgcaa accttacagg cggaagagtc
     7321 ggcgagactg gcgctgtctg ccccgccata tggcaatacc attgcccgcc agccagtttc
     7381 cgccagcgac tttgccctgc aattcacgcc cggaaaacgc tatcgggtca ttgacgtcat
     7441 ccataacgaa ttgattacgc actcccgctc cagcgtctac agcgaaaatg gttttgatcg
     7501 cgatgatgtg tgctttattg ccgtacttga gcgttacggg caacggctgg ctccggcctg
     7561 tggtttgctc ggcggctttg gcctgaatga aggcgcgctg gctgcgacag tcagccatga
     7621 cagccataat attgtggtga tcggtcgcag tgccgaagag atggcgctgg cggtcaatca
     7681 ggtgattcag gatggcggcg ggctgtgcgt ggtacgtaac ggccaagtac aaagtcatct
     7741 gccgttaccc attgccgggc tgatgagcac cgacacggcg cagtcgctgg cggaacaaat
     7801 tgacgccttg aaagccgccg cccgtgaatg cggtccgtta cccgatgagc cgtttattca
     7861 gatggcgttt ctttctctgc cagtgatccc cgcgctaaaa ctaaccagtc aggggctatt
     7921 tgatggcgag aagtttgcct tcactacgct ggaagtcacg gaataataaa aaaagcccgg
     7981 cgtcatgccg ggcaaaagtc accagttacg tttatgccac tgtcaactgc tgaatttttt
     8041 tctcgcggcg gattttgcgt tcttccatta ccgccactat cgccatcagg cagatacaac
     8101 caatcgcggc gatatccagc gcggcgaagg tgcctgccca gccggtaagg ccgaataccg
     8161 gcgtcccatc ggcaatcatt cccagaccta acttggcaaa gctgtcacca atcaggtaag
     8221 caaaggtgcc tttaatacca tcggcagcgc caatcgcttt tttaggtaca aagccaacag
     8281 cagccacacc aatcaacaat tgcgggccaa agaccaggaa acccaacgca aagagagaag
     8341 ccagatagat atattcgtta ctggcatgtt gatacacacc gagcgtggcg ataatcagcg
     8401 ccagcgcgat gcaggccacc aggccacggc gaccgttcgc caggtcagag agccagcccc
     8461 acagcagcgt accgaccagc gcaccagctt caaacagcgt aaagccctga atcgccaccg
     8521 ctttagagag tttcagttcc tggaacgcgt atacggttga ccactggtcg ataccaatac
     8581 gtaccacata gaggaaaatg ttggcgaagc acagcagcca gatcactttg tttttcagca
     8641 catactcaac aaagatctgc cacttggtca tatcggtaga ttctgtctct ttgtcctctt
     8701 cgctgacctc ctcgccgaac agttcttcag ctttgccgag gccataagat tccggggagt
     8761 cgctaccgta acgcaggccg ataaaaccga caatcagcgc gataatcgac gggaagataa
     8821 acatgccgat gacatggcca tcgaacaggt aatttgcccc gaacagcgcc acacctgctg
     8881 cgcctgcacc gccaaggttg tgagaaatat tccagaaacc gaggaatgtc ccgcgtttac
     8941 gacgcggcgt ccatttggtg atggtggagt aactgcacga accgccggta ctctggaaaa
     9001 agccgcttaa ggcgtagaag gcaatcatca ggaacaggct aaccgagccg ctgcccatac
     9061 tggcactgaa gcccagcata caaatggcag agaggataag catgaacggc aggaattgtt
     9121 tggtgttttt gccgtcggcg tagtatgaaa ccaacgtttt acccacgcca taagtgatgg
     9181 agaaacccag gccgatcatc cccagctgcg tcatgctcaa cccgtaggtc gaaatcatat
     9241 cgttctgcgc gatgttaaag ttcttgcgaa tcaggtacat cgtcaggtag ccgataaaga
     9301 ccaccaggta ggattgcatg aacggtttga accacatttt gcgccgcact tcgagcggaa
     9361 ggtccagggt cggcttgcga acctggttta ggaaagccag catgggttac tcctgaaatg
     9421 aatacctgcg tgaggcatgc attgtaaaaa tcagcagcaa atcaggcctg agatagcgtc
     9481 caagaaaaac ctggaaaatt tcttagtttt gccggatatg aagtgaaaag gtgagatgca
     9541 tcacgcttca cgcggtgtct gggcgttcaa aaagggcagt aacagcagtg cggaaatccc
     9601 ggcggcgata gcgataacca caaaaaatcc gctccagtgc caggtatcga gcactttcgc
     9661 cagcggccaa ccagcaagcg acgcccccag ataagcaaac aagccgacaa accccgtcgc
     9721 cgcccctgcc gcctctttgt gggaacactc tgccgccgcc ataccaatta acatctgcgg
     9781 gccaaagaca aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt agctggcaaa
     9841 tggcatcagc cacagcgagc cgactgaaag caaaattccg gcggcgaaaa tcaaattcat
     9901 cggcccacgg ttgccgttaa acaatttgtc cgagccccaa ccggctacca gcgcaccgat
     9961 aaatccgccc agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat cgacgcccag
    10021 cgtctcggac atatacaaat tgccccagtc gttgatcgcc gcccggacca catagaccag
    10081 cacatagcaa aacgaaagca gccagatata cggattcagc aacacatatt tggtgaggat
    10141 ctctttacgc gtcaaccctg ccccttcttg ttgttgagca atttccagcg cgtcgtgccg
    10201 ccattcaccg accgccggta aacctaacgc ctgcgggcga tcgcgtagcc gccagcagag
    10261 aaaaatcccc acgactatcg ccatacaacc agcaatcatc atcccggcac gccagccgta
    10321 atgcagcgcg ctcgctgcca tcacaatggg aatgagtgcg ccgccgacgt tatgcgccgt
    10381 gttccataat gcccaccaac cgccgcgctc ggtacgtgaa taccaggccg ttaacagacg
    10441 cgcacacacc ggtgaacccc agccctggaa aaaggcgttc agcacccaga gcacggcaaa
    10501 cgcccatagc gacgtcgaga agccaaacag aatgttcatg atgcccgtgg cgataagccc
    10561 tatccccata aaataacggg catttgagcg atcgctgaca atgccggaga caaacttcga
    10621 cacgccgtaa gtaatgtaaa acagggtcgc taacaggccg atatcgctac ggctgagcac
    10681 gccgttagca aggatttctg gtaccgcggc gttaaaactt ttccgcgtga agtaaaacag
    10741 cgcgtaaccc agccagatgg tcagcagaat atgccgacgc cagtagcgat aacgggcatc
    10801 aatttcatgt ttatcagtca ttaatggcgc atcggcaggc gctttcagaa acggcaacat
    10861 catcgaacct tagacataac gttgaggtag agaaacgctg acacgcgtgc cgtgcagaca
    10921 ggaaatgtgt aatgtgccgc ccagcgccgt tacgcgctcg cgcattccgg tgaggccaaa
    10981 accttgttgc ccggaacccg gcggcaaacc gctgccatcg tcttcaatca ccagcatcaa
    11041 ccgttcatcc tgctgccagc cttgcagcgt aaccgcgctg gcatcggcat gtttcacaat
    11101 gttgttcagc ccttcctggc agacacgaaa cagcgtcacg cgctggtttt cgcttaatgc
    11161 tgattcatcg atacgccatt cgagatggct gacaatgccg cgcccttcca gctccatttc
    11221 ccgcatcagt gagcggatgg cctgctccag ggtgagatca tccagctggc gcggacgtaa
    11281 ccgtcctaac aaacggcgca ccgcgtcgta aacgcccagc gacagttgtt cgatgagctg
    11341 cccgctctgc ttcacactgg cgttatctgc cgccagccgc tgaacaatgc ccgcctgagt
    11401 acgaatagca gtgatggtct gaccgatatc gtcgtgcagc tcacgcgcca cgtcgcggcg
    11461 cacgctctct tcggtttcta acaaacgttc agccagatgc tgattgcgcg ccagttcctt
    11521 ttgtagcgac tggttaagtt cacgcaaccg ctggatgcct gcgcccagta acaaccctgt
    11581 cagactttgc accagcaacg agagtaataa atccaccaga tgatcgcgcc aggtttgact
    11641 ggcgatcagc gcgatggcgt tcatcaacgt cgcaatcagc gctccttgcc agccatagtg
    11701 ccaggccagc gcgataatcg gcagcgccag acagaatggc gtaaagcgcg acagttcgtc
    11761 cggcaatccc aactggagcc agagactgat aacaaacagc agcaagtacc agaccagatg
    11821 ccgcccgcgc cagttgatgg gctgagaaac cagtgacggg ccgagcggca gccaggtgtt
    11881 attggcgaga tagtgccaga acaccagaca tatcggggcc agcgtcagac cgccagttaa
    11941 agtcagcaac agcgcattcc acgactcttt gccgtgccaa agccagggca gcgactgcaa
    12001 caacgccgcc gccgttaacg ccgcaccctg taacagcaag gtgcgccagt cacgctgatg
    12061 gcgatagcgc gagatcagcg ctacgggcag taacgtcagt aaactaccga tcatcaataa
    12121 cggaaaatgg gttaaaccga ccgcctgcgt tagccagtaa atcagcagcc actccgcacc
    12181 cagcaatacg ggccagtatc cgcgcgggca ttgcagcatt agccccagac gcagaccaaa
    12241 cggaaataac agcaccgcca tatcagggcg ctcaaccaga tgcaggctga tactccatag
    12301 gcaaaaccat gcggcagaga agataaaaaa gcaggcaata acggtaatta agcgggagaa
    12361 caacgtcttc atcaccagcc atcaaacatg cggcgcgcca gctctacgtc gttactgacg
    12421 cccagttttt ccatcagatt ggcgcgatgg acgtgtaccg ttttcggtga caagcccagt
    12481 tcggcggcaa tctctttcac cgccattcct tgcgccagtt tttccgccac ctggcgttca
    12541 cgtttggtca gcgggtcctg acgaccggat gccagtttaa tggcaatatc cggcgtcaga
    12601 taacagccgc ccgtggcaac cgtatgcacc gcagcaatga gttcatccgg gctacagcgt
    12661 ttggaaagaa agccgcgtgc ccccgcgtta agcgcctgct caaccagcgc aggactgtcg
    12721 tgaacggaga gcataatcgt cgccatacct ttcggcagct ggcttagcag ctccagaccg
    12781 gagatatcgg gcatggagat atcgcaaata cacacctgca caccgcgccc cggcagcccc
    12841 gccagcgcct cgcgccccga accaaactcg gcaactacct gcaaatcagg ttccagcccc
    12901 agcagctgcg caaagccgga gcggacgatg aggtgatcgt ctataagggc aacggtgatc
    12961 atggtcttgt cctggcgggt aaaaaaaaac gcgcttacct taacgataag cgcgatgttg
    13021 ttcaagcctt gagcggttac tgaaaaaaca ccgcgatctt gttaaacatc gtcggatcgg
    13081 actgattacg ctgcactttc acgacatctt ccagcttatc gatttggctt atcatctgct
    13141 ccagacgctg gtcgtcattg accagtagcc agatatggct tttgtcgctg tcctgaatcg
    13201 gcagacaaag aatgccttca acgttaaaag cgcggcgggc aaaaaggcca caaacgtggg
    13261 tcattacgcc cggatggttg cgaacggtga gctccagaat tacgttgtta tgagttgtgt
    13321 tttgcatggc ttattccccc accatttcag tattcgccgc acctggcggc accatcgggt
    13381 aaactttttc ttcggcatca atgcgcacat ggatcagcgc cgggccaggg cgattgatta
    13441 tttcctgcaa tgcagcctgc ggatcggctt cgttattcaa atcacaggtt tcgaggccga
    13501 atccggcggc aatctgcata aagttgattt tgcccggata ggtggcggca aaaacgcctt
    13561 gctcgtagaa cagactctgt tgctgatgca ccagccccag cgcttcgttg ttcatcagaa
    13621 tgattttgac atccagctga ttttcactgg cggtcgccat ctcctgaata ttcatcatca
    13681 ggctgccgtc gccggagaaa cacaacactt tgcgatccgg gttcgccagc gccgcgccaa
    13741 tcgccgcagg caggccaaaa cccatcgtgc ccagcccacc ggaggtcagc cactggcgtg
    13801 ggcgattgag cggataagct tgcgcggtcc acatctgatg ctgacccaca tcggtggtga
    13861 taatcgcatt gtcatcgaca caggcggcaa cggcgttgat caggccgtaa tggcttaatg
    13921 gatcgcacgc tttcgggatt ggacacggga attcgcgctg caaatccgct accaactggt
    13981 gccactctgc acgcagttgc gcttccacct gcgggatcaa ttgcgccagc acgtcatcaa
    14041 catccgcctg aatcgccaca tgcggctgct tgattttacc cagctctgcg cgatcgatat
    14101 cgacatgaat aattttggca ttcggacaga actgctcggt tttgccaatc gcccggtcat
    14161 caaaacgcgc accgagcaca atcaacaaat ccgcctcttg caaaatatag ttggtactgc
    14221 gcacgccgtg catccccagc atacccagcg acaacggatg cgcttttggc agcatgccca
    14281 gcgccattaa agtcatggtg gtaggcagtt gcgctttctc cgccagttca cgcacccgcg
    14341 caggcgcatt gatcacacca ccacccagat aaagcaccgg gcgtttggca gcgttaatca
    14401 ttgtagctgc gtcacgaatg ctttcttcgc taaaggaggg tgcagcggct ttttctgcca
    14461 ccgcgggctg agcttcaatc tcaaaaaccg ccgtttgtac atccttagga atgtctatcc
    14521 acaccgggct agggcggcct gattgcgcaa tgcggaaggc atcgctcatg acctgtggga
    14581 gctcttcgat atgtctgacc agatagttgt gtttggtgat ggggatagag atgccgtagg
    14641 tgtccacttc ctggaaggcg tcggtgccga tcatcgaggc gggaacctga ccagtgatgc
    14701 aaatcagcgg gatggagtcc agccgcgcat cagcaatggc ggtcaccagg ttagtcgcac
    14761 ccggcccgct acaggccata cagaccgccg gtttaccgtc ggtgcgcgcc attccctgag
    14821 cgataaaccc cgcgccctgt tcatggcgag ccagaatatg gcggatttgc gtgctttggc
    14881 ttaaggcgtc gtaaacaggc aggatagaac cgcccggaat gcccgtcaca atcttaatgc
    14941 cctgctgttc caggaaatga acgataaatt ctgcgccggt aaagcgctta cgcgtcgatg
    15001 ttgtgcccga acttgccatg ctccagtcct tttcttctgg gccgactttc cgggaggtgc
    15061 ttaaacgaaa aaccccgccc ggtttgcgcc ggcggggttt tggaatcgtg tgttgttcca
    15121 gtccctacgg cgcattgccg acgaccacca ccacacgcac gacgaccact gcggcggatg
    15181 gcgcagttgg tagtagtttt gcgttgaaca tggaagtagt cattggggac cttgttggtt
    15241 ttgtgtttaa caatatttat acaagcacag ctttacaggg gagacaatgg aaaatttttc
    15301 agcaagggaa aattgagggg ttgatcacgt tttgtactga attgcagata acaaaaaacc
    15361 ccgccggagc gaggtttcgt cagccgcctg cggctggtaa ccgcaaagca cactgtatta
    15421 tgtcaacact gaaagtatac gtgttccgcg cagaacgcgc aatttcggca cgaattttga
    15481 cgtatttagt gcatagttga gtatcgttca cagtttgcgt tttgtccaaa tattactgtt
    15541 tatttataca gtaaacttct ataatatcac tgttcgcaat gtgttatgcg ggggccgcat
    15601 cgttacccgg cgcactaagt cctggctgaa acgggtggtg ccgtcagcgc cttaaccccg
    15661 cgtgagcaca ctgtgttatg tcaacaagca caacgtttct ccttgagata ccgcgtgcac
    15721 aacagctggc aacaggcagc ggaaaggtac gtcagctggc agtgctcctg aaccacagga
    15781 gacgcgtatg agcctggtgg atatcgccat tcttatcctc aaactcattg ttgcagcact
    15841 gcaactgctt gatgctgttc tgaaatacct gaagtaattc agattcaagt cgcaccaaag
    15901 gggagcgggt aaccgctccc cttttatatt tagcgtgcgg gttggagtcg gatgcgatgc
    15961 tgacgcatct tatccgccct accatccctc ccggcaacat ttattgccgc ttttgtttac
    16021 atattctgcc gctaaacaat tccccattcc tggcgtatat ctggctaaca ttcatcaatg
    16081 tgatagattc ctctcccgca tttatgggaa tgcgtagtga cttattctaa ttatttttat
    16141 aaaagcatcc gtgataatga aaaggcaaag aaacgtcaat ttgttattga tgttggtatt
    16201 actcgtggcc gtcggtcaga tggcgcaaac catttatatt ccagctattg ccgatatggc
    16261 gcgcgatctc aacgtccgtg aaggggcggt gcagagcgta atgggcgctt atctgctgac
    16321 ttacggtgtc tcacagctgt tttatggccc gatatccgac cgtgtcggtc gccgaccagt
    16381 gatcctcgtc ggaatgtcca tttttatgct ggcaacgctg gtcgcggtca cgacctccag
    16441 tttgacggta ttgattgccg ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt
    16501 aatggcgcgt actttgccgc gtgatttata taaacggaca cagttgcgcc atgctaacag
    16561 cctgttaaac atggggattc tcgtcagtcc gttgctcgca ccgctaatcg gcggtctgct
    16621 ggatacgatg tggaactggc gcgcctgtta tctctttttg ttggttcttt gtgctggtgt
    16681 gaccttcagt atggcccgct ggatgccgga aacgcgtccg gtcgatgcac cgcgcacgcg
    16741 cctgcttacc agttataaaa cgcttttcgg taacagcggt tttaactgtt atttgctgat
    16801 gctgattggc ggtctggccg ggattgctgc ctttgaagcc tgctccggcg tgctgatggg
    16861 cgcggtgtta gggctgagca gtatgacggt cagtattttg tttattctgc cgattccggc
    16921 ggcatttttt ggggcatggt ttgccgggcg tcctaataaa cgcttctcaa cgttgatgtg
    16981 gcagtcggtt atctgctgcc tgctggctgg cttgctgatg tggatccccg actggtttgg
    17041 cgtgatgaat gtctggacgc tgctcgttcc cgccgcgctg ttctttttcg gtgccgggat
    17101 gctgtttccg ctggcgacca gcggcgcgat ggagccgttc cccttcctgg cgggcacggc
    17161 tggtgcgctg gtcggcggtc tgcaaaacat tggttccggc gtgctggcgt cgctctctgc
    17221 gatgttgccg caaaccggtc agggcagcct ggggttgttg atgaccttaa tgggattgtt
    17281 gatcgtgctg tgctggctgc cgctggcgac gcggatgtcg catcaggggc agcccgttta
    17341 agcgcacgtc accgcagcat cgtcatcagc tccatgggag aacgatgctg ctttatcaga
    17401 tcacgcatca cccgcatatg cggtgcggag taagaataaa acgcctgata gcccgcacaa
    17461 agcacgctgt ctggcgtctc ctgccgagcg tgcgggcaca ggcgatgcca gtcgcacgcc
    17521 tggcagtcac ggcgcggttg cgggttttcg ctgccacacc atttttgtaa ggtggcgtca
    17581 aaaagctcaa tcgagatacg actgatatct gcccgcaccc aacactcaaa cagggcaatt
    17641 aaaaatttcc cccacagctc tgctgtcacc gaagcggccg tcagatcgcc ccggtcatca
    17701 aattcaagat ggggaatgag ctgtacggag cggatgcccc gctgtttgaa attgtgataa
    17761 aaatcggcag gttgcagagc atccttgtac tctacaacca gcttatgcct gtcctcttca
    17821 gcatcaatga cctgacttcc tgtcatgcga ctctctcaat aaatacgata agttgatgta
    17881 tatgcgttac agcaaacagt attgcgagag ataacaccgc gagggagatc aaaaatttgt
    17941 cacggactac gtgaaattgg gaaaaatcgc tattcgtgac atccattaaa ttgcgattac
    18001 gggtgtagtg ccagaggatc agcgccacga tggcgaggat gccaatcgaa atccagaata
    18061 acatgcccgc ctggtgccag ttgtgtttga tagccaacgc catcaacgcg ccgtagccca
    18121 gcatggtacg aaaccacgcc agtgatgtgc gctccggctg cagccctggg tcggcaatgc
    18181 gtctggcttt acggctatcc ggcatacaac accagtccca taacgatcac cgccacaacc
    18241 atcaatatta agctgatgat taataagctg ttggtgtagg gcaggtcctc tttcagacgc
    18301 atcgcttttt cattgcgtaa ccagcgcagg tagccataca ttgctaaccc gccggaaaac
    18361 agacacaaca acaacgccag cagttcacga atgacgggcg tggcgaaatc tggcgcaagt
    18421 tgatcaagcc caacccccgc cgccagaaat cctagtgcgg tacggatcca cgtcagaaaa
    18481 gtacgctcat tagccagcga gaagcggtaa tcgggcgctt ctccaaggcg ggaaattttc
    18541 attgttgctc ctttgcaatt tcactaacgc tctatcttta gcggtttttt ttaagaattt
    18601 cgtccgcccg tttcctgata tgcttttgcc atccgctaca aggaggtcag atgggtatca
    18661 tagcgcaaaa taaaatcagt tcattgggca tgctgttcgg tgccatcgct ttaatgatgg
    18721 ggattatcca cttttcattc ggcccttttt ccgcaccacc gccaacgctt gaaagcatcg
    18781 tggcggacaa aacggctgaa attaaaagag gtcttctcgc cggaataaaa ggcgaaaaaa
    18841 ttaccactgt ggagaaaaaa gaggacatgg atatcgataa aatcctcgat caaagcggta
    18901 tcgcactggc cattgctgcg ctactttgcg catttattgg cggtatgcgt aaggaaaatc
    18961 gctggggcat tcgcggcgcg ctggtttttg gtatcggcat cgtgtgcagc attttgctga
    19021 tattcctgat tttttccttc ctgaccggcg gttcgctggt ttaagcagcc tgaataatgc
    19081 ccgccgggtg atcatcagcg ggcattatcg tcagaatttc tgttttttct cctccacctt
    19141 cactccttgc gtcggtaggc cggtgtcata gtcgcgtacc actggcgaat agccatcttg
    19201 tgggcgtggg cgaaacgccc ccatccagcg cggtcgtgca tctttacgcc acggacgcag
    19261 actccattgg taactgcgga acggatcgcg aattttgtcc atgtaatcca ataaggcgtc
    19321 atgcattttg ctgcgaacgt ctgcaaaacg gatatcatcg atcaggttat gcatttcatt
    19381 tgggtcatta cggcgatcgt aaagttcatc actggtgaag aggttgagca ccagtttaaa
    19441 gtcatccgtc acccagcaac gcaccggaat aaaaccgcca aagctgtcat gctcaatctc
    19501 gtagcggtta aattccacca tcacgccgcg tggctctttc acggcaagga tattttcccc
    19561 cggcagaatc tctggttttt caatatctgc cagcgccatc attgtcggca gtaaatcaat
    19621 atgactgact ggcgtatcga cctgtcgccg ctcctttact gatcagctta tgtgcgccca
    19681 tcatttcacc gtgatcggag gtataaatga cccacgtatt ttcacgttgc tctggcgtta
    19741 aggcattgat gacacgtccg atttggtcat caacaaagtc attgcaggca aaatagagcg
    19801 gatggtgata aagcccgtca tcaccgactg gcgatggcat cgcctgcgcc cataagcggt
    19861 gatgttccgg tttgttcgcc aggtcatcct gtgatttctc gccaagttcg tagtaaaaat
    19921 cagcgtattt ctctaaatac tccaccggac aggtgaacgg gtgattcggc tcatcatacg
    19981 aaaccaccat caggaagggt tcctcggcgc gcacgggctg ttgcagaaaa tctaccgctc
    20041 gattgctgat gcggtgcgcc caggtgaagg tttcgtcaat atggttcgcc tgtaaatctt
    20101 cgacgctgtt taggccattg cgccacaggc tgatctcttt ttccgtcagt tcgctaagat
    20161 agttcgcccc atcaaaccag taatcagcat cccactccgg cggacactcg ccagtgccga
    20221 aatagtcatg accgtcgaga tgccatttgc cgatgtaaca ggtgtgatag ccggcatcct
    20281 taaagtagcg ccccatagtg gagatgtttt tgcctggcgc gacgttgttg gtccacgggc
    20341 cggactggtt agcgtagata ccggtgaata gtccggcgcg tgcaggcgta caaaccggtg
    20401 aacaagtgta ggcggaatta aagcgaatac cttccgccgc cagactatca atattttgcg
    20461 tattcagcgg tttaccgcta tagcaaccga ccatattggt ggcctgggta tcggtcaatt
    20521 ggggcgtttc ataggctatc cttcgcgtcg ggagtaaagg ttacggctgg gtcgtgacga
    20581 tgccgccagc tgtcaaaaat caggtagaca atcactacgg cagcgatgaa ataactgatc
    20641 atcgctaacc agcgcgtacc gtagccgccg aattcagcca gcccgacata gacgccaatc
    20701 atcgcgaaca ggataccaat tgacgcgatc ttgacgtttt tccacggttt catgtccacc
    20761 gcaaacgcat ctttgaaggt gaacggcgtg gcgcgcggtt tgataaaacc gatcaccagc
    20821 atcacgacca cgttgatgca gaacgtacag gccagcacat agaggaaatg gaagtcgaac
    20881 ttcaccagat agttgatggt gatgtagctg attatgccaa tccccatcgc cacttttgcc
    20941 gccagcgccg ggatgcgcgg gaagaaaaag cccataatga tgatggtaac cagcggcacg
    21001 ttgtaaatgc cgttgagctg tttcatccag ctatacagcc cctgcggcgc gttggcgatc
    21061 cacggcgcta ccaaaaccga aacgatagcg ataaagaaac caaatttgcg cccgacggtg
    21121 accagctgct gcggctcggc attctggtta atgatgcgac ggtaaatacc catactgaat
    21181 aatgtactgg cgctattcag aaagccgttg aaggtactga tcaccgcacc aaataacacc
    21241 gcgccgaaga aacccaccag tggcactggc agaacgttat tgaccagcgt cgggtaggcc
    21301 atgtcggctt tcggtagatc ctgatacaga tgaaacgcaa tcaaccctgg cagcaccagt
    21361 accagcgggt cgagcatttt cagcaccgcc gttaacagcg cccccttttg cccttccgcc
    21421 aggctttttg acgccagcgt gcgttgcacg atgccctgat ttgtacacca gtaaaaggtg
    21481 ttcaccagaa ttaaaccggt aaatgccgcg ccaatcggca agggatcggt cgggccaccg
    21541 actgagttta atttctcggc gtgaacggtg gtaatttgct caatgccctg cataaagctg
    21601 cccttgccca tcgcgatcag gccaaatacc ggcaccatca acccgccgat aaccagccca
    21661 ataccgttga tggagtccgc cactgccatt gcgcgcagtc cgccgatcac cgcatacaaa
    21721 attcccgcca gaccaagcaa aattaccagt agccagatag ccgcaccgtg agaaatctgt
    21781 agcgattccc cgacgtgaaa caggctgttg agcgccaacg cgccggagta gagaacaatc
    21841 ggcagaaagc agacgccggt ggcaatgagg aagcagaagt cgatgataat acgcgtcgtt
    21901 ttatcataac gttcctccag aaaatcgggg atggtggcaa tgccgcgctt cagatagcgc
    21961 ggtaaaaaga ttagcgcgag gaagatcagc gtcaccgctg aagtcacttc ccagcccatc
    22021 accgacatgc cgcttttgta ggcctgcccg gaaagaccaa ccagttgttc cgtggaaagg
    22081 ttggttaaca ttaacgaagc ggcaataacc ggcgctttta gtgaacgtcc ggcaagaaaa
    22141 tagccttgtt gcgatccggt atctgttttg cgaaccttcc actaggtgat caccgccacc
    22201 agcagcgtaa aaccgacaaa actcaagatt tgtaacgaat tcatctcata gcccttattt
    22261 atgtttatgt cccggacgca tgaatatagg gagaggtaaa atgtgtcggg tcaggcagtc
    22321 agattcttaa aatacaaacg tcgtatccct gaacggattg ttttctgtta agttcaggtt
    22381 gtgagcataa ttcgccggag ggatattaaa atgaatggaa aattgcaaag ttcagatgta
    22441 aaaaacgaaa ctccgtacaa tattccctta ttgattaacg aaaatgtgat ctccagcgga
    22501 atttctctga tctcgctctg gcatacctac gccgacgagc attaccgggt gatctggccg
    22561 cgggacaaga agaaaccgct tatcgccaac tcatgggttg cggtgtatac cgtacaagga
    22621 tgtgggaaaa ttcttttaaa gaatggcgaa caaataactc tgcatggcaa ctgtattata
    22681 tttttaaagc caatggatat tcactcttat cactgtgaag gtttagtctt ggaacagtac
    22741 tggatggaat ttacccccac cagtatgatg gatattcccg ttggtcagca aagcgttatt
    22801 tataatggcg aaatttataa tcaggaactc accgaagttg ctgagttaat aacttcacca
    22861 gaagcaataa aaaataatct ggcagtcgct tttctgacga aaattattta tcagtggatt
    22921 tgtcttatgt ctgcagatgg taaaaaagat ccacaacggc ggcaaattga aaaattaatt
    22981 gccactttac atgccagtct gcaacaacgc tggagcgtag ctgatatggc tgccacgatc
    23041 ccctgtagcg aagcctggtt gcgtcgtctg tttttacgct ataccggcaa gacgccgaaa
    23101 gaatattacc tcgatgcgcg tctggatctg gcgctatcgc tattaaagca acaaggaaac
    23161 tcggttggcg aagtcgctga tacgctcaac ttcttcgact cctttcattt cagcaaagcc
    23221 tttaaacata aatttggtta tgcgccgtca gccgtgctaa agaatacgga ccagcaccca
    23281 acggatgcca gtccacacaa ttaacgtaac tctggccagt acgctttgtt ggcctccagc
    23341 aactcatcca gaatagcttt ggcgaccgag gcgctcggta ccgttttcga caacgtaatc
    23401 gcctgccaca ggtgctgata tgaacgctgt tcccaggcgt ccacgaccag tttttccacc
    23461 gccacttgct gactcattaa ccctttctga aactgcggga tatcgccgac caccagtggt
    23521 tcaggccgta gtagcgcacg cgcatctgtt tgcgatcttg cagcccaaca atttgcgcca
    23581 tccggctttc aataccgatt ggcatgtcac agatgttgag gattttcgca ttcgggcgca
    23641 gacgacgcgt ggcttctgcg acaattgctg ccgggttgga gtagttgagc atctaggcgt
    23701 ttggtgaata tttttccata taatccacca gttccaggac gccgccaatg gaacgcatgc
    23761 cgtacgctat tccgccgggt ccgcaagttt cctgaccaac aacaccgtgg cgccgtggga
    23821 ttttttcatc cagttcgcgc atcgggtact tgccgacgcg gatgtgcgcc ataacaaaat
    23881 caacgtcgct gaacgccact tcaggatcgg tggtgtaact aaacgcaatg tccggcgctt
    23941 tttctttaag gatgacttta caggcttcgg caatcacttc ctgccgcgcg ccatcgttat
    24001 cataaaattt cagtgcacga agcgggaaac ggtcctgatt cgccaggagc atcaacacga
    24061 tgcctggcgt aaaggtgctt ccaccgcctg cgacaaccac tgagaatttg gtcattatac
    24121 tgcctccgta atggcaacat tttctgctga ttgatgagaa ttaattaagc tatcgagctg
    24181 ttcacgcaat tgggatacat gcagaccaat gatcacctga atggcatcgc cactacggaa
    24241 gacgccgtgc gctcccagct ttttaaagac ttcgtcatcc agcgtttgtg acatgtcatg
    24301 cagtgcaata cgtagacgcg tcgcgcaatt gttaatgctg gagatattgc cgaccccgcc
    24361 cagggcttgc aggataccgg cagcctgatc cagctctttt tttggctctg cagcggtggt
    24421 ttagcctcgc gaggctttgt attcggcttt tgagtagagt ttcacttccg catcttcacg
    24481 tcccggcgtg cacatgttga actgcagaat cagggggcgg aaaaccacga agtacagcag
    24541 ggtaaagcac aacccaatgg cgatttgggt cagcatcata tccgcgtggt tgctgaacat
    24601 cgggatccag ttttgcggta aaacctggtc aatcagacct ccgcccatgt tgcccaccac
    24661 accaaagaga tacattacgg tcgacattga ggccgccagc acggcgtgta ccgcaaacag
    24721 caacggtgaa atgaacagga aggtaaattc cagcggttcg gtaattccca ccagcatggc
    24781 ggttaaggtt gcgggaatca gcaagcccgc cacttttacc cgattttccg gtgcggcagt
    24841 gaagtacatc gctaaagaaa tgcccacggc accaaagatt tttgagttac cgtgcagggc
    24901 aaaaccgcct tccgggaaca acgatttcag cggctcggcg ctcagactga actcttgcag
    24961 atgctgcgcc cagtacatct gaatgccgcc ttcaacagct gccggaccaa agataaacgg
    25021 tccgtagatg aagtggtgta aaccggttgg gatcagaata cgttcgagga aggtgtaaac
    25081 ccacacccca agcgcacccg ccgaacgcag gaacgcttgc agagattcaa tccccatttg
    25141 tacttttggc cagccgagca atgtcagcca ggcacagggg atcatcacca ggaaggcgat
    25201 aatcaccaca taagacgtcc cctggaaaat gccgagaaaa accggcagtt ttttatcgaa
    25261 cagacggtta tgcagcgccg tcacaatgcc ggaaatgata attgcgccga taatgctggt
    25321 atcgagggtt ttaatcccgg ccatcattgt cagaccgcta cctgccaccg cgtcctgagt
    25381 gaaatcgacg ccgaagtagc ttccccaggt cattcccatc gcattgatga aatagttcca
    25441 ggtcaggaaa ctcaccatca ccgccagaca agcacgtccc tgcgcttgct tagcaaggcc
    25501 aatgggtaaa ccgacagcaa aaatcagcgg catattacgg aataccgtcc aaccgccctc
    25561 ttcaataatg tgtacgattt gcgcgaataa actgttcgga tcggtcagtg attccccgac
    25621 aaacatcggg ttttgcagca agatggcaag acccaccaca atcccggcga agggaaacag
    25681 cagcactggc gtgaacatcg cgccgccaaa gcgttgaatt tgactgagca tttccacatc
    25741 ctcttttctc aattctgtaa gtaggaagcc tgattggtta tgcggcagac ctggagcgag
    25801 gataggaaga tgcgatcggc agaacatgac gacccaatgg gattcgtgat cgctttcatg
    25861 cttttcgttg ggcggtagag atctacgttt tcgcgaaaaa gtagacatct gtgagcacag
    25921 gtacacagca tcgaacacat ctttaaaaaa aagatttttt tcaatcgatt aagcagaact
    25981 tgtgggcgca ttacccgtgc ttgcaggcaa aaaagagagc tagagatgat ctacaaaagc
    26041 attgcggagc ggttaagaat tcgacttaat tccgcggatt tcacgctaaa cggccttctt
    26101 cccggtgaaa aaaagctggc ggaagagttt gcggtatcgc ggatgactat ccgtaaagcc
    26161 attgacctgc tggtagcgtg ggggctggtg gtccgccgcc acggcagcgg cacttacctg
    26221 gtgcgcaaag atgtgctgca tcaaaccgcc agcctgaccg gactggtgga ggtgttaaaa
    26281 cggcagggaa aaacggtcac cagccaggtg ctgatttttg aaatcatgcc tgcgcctccg
    26341 gccattgcca gccagttacg gattcaaatc aacgagcaga tctacttctc ccgtcgcgtt
    26401 cgttttgtgg aagggaaacc gctaatgctg gaagacagct atatgccggt aaaactgttc
    26461 cgtaaccttt cactgcaaca tctggaaggg tcgaagtttg aatatattga acaagagtgc
    26521 gggattttga ttggcggtaa ttatgaaagc ctgatgccgg tgctcgccga tagactgctg
    26581 gcgcggcaaa tgaaggtagc ggaacacacg ccactgctgc ggatcacctc gttgtcatat
    26641 agcgagagcg gggagttttt gaattattca gtgatgttca gaaatgccag cgaatactag
    26701 gtggagtacc atttacggcg actccacccg gaaaagagtt aaccgatact ccagaagagc
    26761 accgccagta attggggggt gataatgcgc aggaacatca ctaacggata gacagtggcg
    26821 taagagagcg ccgccgcacc actggttgga tgaagattat tagcaaacgc cagcgccgga
    26881 ggatcggtca tggaacctgc cagcatcccg cacatggtca ggtaattcat tttggctaac
    26941 atccgcgcca gaatgccaac agtaatcagc ggaacggcgg tgatcagggc accataacca
    27001 atccagctta gcccttcgcc attgaccagg gtattcacaa aatccccacc agatttcaga
    27061 ccaacgaccg agaggaacag cacgatcccc agctcccgca gcgcgaggtt ggcacttggc
    27121 ggcataaacc agtacagctt gccgatactg ccgatacgcc cgaggatcaa cgccataatc
    27181 agcggaccgc ccgccagccc cagtttcaac gcggccggga atcctggcac aaagacggga
    27241 atagaaccta acaatacgcc aagcccgatg ccaataaaca ctggcagcat ctgaacctgt
    27301 tgcagttttt gctgcgcatt ccccagcaca ttggcaacgg catcaattgc ggacggacgc
    27361 cccaccaggt tgaggatatc gccgaactgc aggctgatat cgccactggc gaccagttcg
    27421 accccggcac ggttcaggcg cgagataaca acgtcatagc gttctttaaa gtgcaggtcg
    27481 cgaatacgtt ttccgagcac gttttcattg gtgaccacca cacgctcgac gcgcaaatca
    27541 gtgcctttcg tagacagcga ggtatcgacc tcctgaccaa tcaccagttg cgcattatgt
    27601 aaatccgctg gctgccccac cagatgcagc aaatcgccca gttggataat ggtatctggc
    27661 gaaggtactt ttagggtttc ttctcgtttc agacgcgagc agataatttt gtcgccgttg
    27721 agaatcggta catctttaat ggctaaatca tgcaggttag ggttctcaac gcgaatattg
    27781 atagtcttga tcagcgcgcc gccattggtg cgtgaagact cgtgctgctg agcttctgtc
    27841 tcgacattga cgcggaaaat aacccgcaac atccacatgg tgaacaaaat cccgcaaatg
    27901 ccgaatggat acgccatcgc gtaactcatc cccatctgat cgaccatttc cattggtgta
    27961 cccaggtcgc gcaagatctg ctgccctgcc cccagcgctg gcgtattggt taccgcaccg
    28021 gagaaaatcc ccagcactac cggcagtgga atatcaaaca gtttatgcag gatggcggta
    28081 accagaccac cgatgatgac gatcagaaca gcaaacaggt tgaggcgtaa tccggagacg
    28141 cgcaatgagg caaagaagcc cggccctacc tgaatcccga tggtataaac gaacaggatc
    28201 aggccaaatt cctgaataac atgcagcata tcgctactta atgtcatccc cgcctgagaa
    28261 acaaaatggc cgacgatgat cccaccaaac agcacgccgc caatacctaa tcctatgccg
    28321 cgaaatttga cgttgccgat aaacaaaccg acgactgcca ccaaagccag aatactgacc
    28381 gttaatgcta tatcactcat cgtccatttc tcttgagata acattttaat tgctacggat
    28441 tatggctgaa tcggtgaagc agatttgcct tatagcgcac attatcacgg gaaagaacgt
    28501 gccgaaatat cttaaacagt cgaaatttaa cgcattgttg aagaagaaag acaaaaaaag
    28561 ccccaccaga atggcggggc aaagagaata gctagttagc tatttaacgc gggacgttcg
    28621 ctgatagcga tacgctgcgc tgcgatgggt tctggctcat tacgaattaa atcaatatgc
    28681 agtaaaccgt ttacgaaggt tgcgccagag acttccatat tttcagccag cgtaaaactc
    28741 aggctaaatg gctgattcat aagcccttga tgcagccatt ttttctcttc ttttggctgc
    28801 tccggcgtgc cttttacgct caggcgcgta ccttccagtt gaatctctaa atcttcctga
    28861 cggaaacctg ccagcgcaag ggtaatgcgg tagtggttat cgtcgctttt ctcaatgttg
    28921 tacggcggga agctctggct ttcaccggcg ttttgcagtg cgttggccag tttgtcaaaa
    28981 ccgatccatt gacgcatcag tggggataaa tcgaagttac gcatagtcat ttctccttct
    29041 aagaagcgag taagtacctg caaatccgaa gattcgcata tgctccctga cggcgagcat
    29101 ggagatgtca ggccgcgcca ggcggcctta gggaattagt tgatttcgat acggcgcggt
    29161 tttttcgctt ccggaatcac gcgttcgaga tcgatataca gcaaaccatt taccaggtta
    29221 gccccacgaa catgaatgtt ctcagctaac tggaatttgc gttcaaagtt gcgttcagcg
    29281 atgccctggt acagataggt gcgctctttt tgttcgtcgg cgtgagcacc tttcaccacc
    29341 agcagattat cctgggcggt aatttccagt tcgctctcag caaaaccagc cacagcgata
    29401 gcaatgcggt aatggttttc gtctaccagt tcaacgttat acggagggta gccgccatta
    29461 ctctggctct ggttgttttc taagtggtta aacaaacggt caaatccaat agcagaacgg
    29521 taaagcgggg ataaatcaaa gttacgcata atcaatagct cctgaaatca gcgagaatgt
    29581 aagaccttcc acaatggaca ggtcaggtag ccagaacacc catcaggcgc gtcctcatcg
    29641 actacgatgt aaaatgggtc tggaaatgaa cttttcaagc cttatcagcg gacttttttt
    29701 tgcagtttat ggtctattgc atagactgag ggggcagcac gaaacgttaa agtgcgatag
    29761 ccgccacagt gcgacgtaat gatgatgtta ttttcagcaa gggatcgcta taactcatca
    29821 ggccaacacc cacgatcaac agatgaattt attatgataa gaaatgtgtt gttagcgttc
    29881 atgatatgca gcggaatgac attactcgga ggatgctcca gcgtgatgtc ccataccggg
    29941 gggaaagaag gaacgtatcc cggcacccgc gccagcgcca ccatgattgg cgacgatgaa
    30001 accaactggg gctctaagtc gctggcgatt ctcgatatgc cgtttaccgc tgtgatggac
    30061 acgcttttac tgccgtggga tgtgttccgc aaagacagct cggtgcgctc gcgcgttgaa
    30121 aaaagcgaag ccaacgcgca agccaccaat gcggttattc caccggccag aatgcctgat
    30181 aactgattta gcgatcggtt tcggtgatcc acagctggcc accttccatc cacgccagcc
    30241 attgaccatc cggcgagaag accacggcgt ccgcagaagg tggattggcg tggttttcgg
    30301 ttaaatactc aacctcgccg ctttgcgcat gggcacaaac aattcgatta tccagcacaa
    30361 agcccaacca ttctcctgac ggatgccagt taaatgcaga ctgaatatcc gttttgttat
    30421 gggttaactt gcgcggttcg ccgccctgtg gcgagataag ccacagttgc acaatgccgt
    30481 tatcatcacg cattaaaaac gcgatttgcg taccctgcgg attacagcgc acccagtggc
    30541 gcgggacgtt gactaacccc ggataagccc gatgatgggt aaaggttaaa cgtcgctgca
    30601 cgacgccacg cggtggcgcg ggtagggttg tttccgttcc acttaacggc gcatcacctg
    30661 ccgatttcca gctagcttca tcttgcggta actcaacgat aaatagctcc ggcactttct
    30721 cgccctttgg cgaaagtgtg tcgccaataa acgccagcgc gtgatttcct acccatcctt
    30781 cttcataagc acgattgatt tcatcactgc caggctgcgg cgtgggcgtg gttttactca
    30841 ccagcacaca ccagtggcta ccgctgtatt cacgcggatg ttgcttttgt acgttgaccg
    30901 ggccaaacgg tgcagcaacc ccgacgtttc gcaaatccag cgccggatcg agttgatgca
    30961 ttacatggtc gttataggta aagctccccc tttcaccgtt cgggctaaag acatgcacat
    31021 ggctgccacc gcgcagcgct cctggcgtat acggcgcggt aatatccatt gcatcgagat
    31081 tgctcacctt gccgccttca gcaatgactc cgcgtcgatg atggaagtcg taatgccatg
    31141 tttcatcagg attttcaggg ccgtgaataa agacatattt ctctgacttc ggatgaacgg
    31201 tcaccacgcc gacgtgtgcg ccctgtgacg cgcgatagat aacctcgacc tcgcctgtat
    31261 ggatattcac acgctcaatg gtctcgccgg taaacgacgc cccagaagga cgcacgtcaa
    31321 ataccagcca ctggctgtcg ggcgtccagg tattggtatt ggtgagcagg tgattacggg
    31381 gagcaaaggt tatctgtttc atggcgcggt cctgatacaa aacggttccc gccatcatac
    31441 ttcacaagga cttcactttc agacaatagt cttttgccat cgtgatgaat gtgagtgaaa
    31501 agatggaaca tttcgacgtg gcgattattg gcctcggccc ggcagggtcg gcgttggcac
    31561 gaaagttagc cggcaaaatg caggtgatcg cgctggataa aaagcaccag tgcggtactg
    31621 aaggtttcag caaaccttgc agattttcag cgtcaaaact gtcgacgtcg ccgcatcgct
    31681 cacgcgtaac taccagcgaa gctatatcaa tattaatcgc catgctttcg acttgtggat
    31741 gaaatcgctg atccccgcca gcgttgaggt ttatcacgat agcctgtgcc gaaaaatctg
    31801 gcgtgaggat gataaatggc atgtcatttt tcgtgcagac ggctgggagc aacatattac
    31861 cgcccgctat ctggtcggtg ccgatggcgc aaactcgatg gtgcggcgac atctctaccc
    31921 ggatcatcaa atccgtaaat atgtcgctat ccagcagtgg ttcgcagaga aacatccggt
    31981 gccgttctac tcctgcatct ttgataatgc gataactgac tgttactcat ggagtatcag
    32041 caaagatggt tattttatct ttggcggtgc ctatccaatg aaagacggtc agacgcgttt
    32101 cacgacgctg aaagagaaaa tgagcgcctt tcagttccag tttggcaaag cggtgaaaag
    32161 tgagaaatgc acggtgttgt ttccctcacg ctggcaggat tttgtctgtg gtaaggacaa
    32221 cgcctttctg attggtgaag cggcgggatt tatcagcgcc agctcgctgg aggggattag
    32281 ctatgcgctg gatagcgcag agattctgcg tgcggtgttc ctgaaacagc cggagaagag
    32341 caacgccgct tactggcgcg ccacacgcaa actgcgttta aaactcttcg gcaagatagt
    32401 aaaaagccga tgcctgaccg caccggcttt aagaaagtgg attatgcgca gtggtgtggc
    32461 gcatattcca cagttgaaag attatccaac gcgcttcaca tcacccacca gcaggatgta
    32521 agagagcgcg ccaatcaacg cgacggcgga gatataaacc agtgcaggcg cgaaaccgta
    32581 accctgcgcc aggtacccca ccaccagcgg aacggtgatg ccgcccagac caccggcgaa
    32641 gttaaacacg ccgccggtta aaccaatcag gcgcatcggt gccagagaag agaccagcga
    32701 ccaggtaatc gaagcaaaac cgttgccgaa gaatgccagc gccatcaggc acataatcat
    32761 catcggatcg ttggtgtagt tagcgcccat aatgcaggtg gagatcagca agccgcagat
    32821 aatcggcgtt ttacgcgcaa agcccagtga aaagccctta cgtaccagca gatccgcgac
    32881 ccagccagag agcaggacgc cgacaaacgc cgcgaggaat ggcaccgtgg tcataaagcc
    32941 tgctttcagc gccgtgattc ctttttcctg ggttaaatag ttcgggaacc aggttaagaa
    33001 aaaccacagt gcagaagcca ccgcaaattg cccaagataa acgccgatca gtttacgatg
    33061 gaacaccagt ttccagtctt tggctgttaa cggctgacgc gcctctttct tcaccggcgc
    33121 atcaccatcc accagaccgc cgccatcacg aatgtaatcc agttcagctt tgctgatacc
    33181 tttggtcagg cgcggcggct gataaacctt aaaccaaatc agcgaccaga taatgccgat
    33241 accaccagtg acaatgaaca cccagtgcca gctcaacatc tcctgaatcc agatcagcag
    33301 cggagtcaaa aacgccagac cgacaaactg accagacgta taaaaaccaa cggcggaagc
    33361 gcgttcatgt tccggaaacc agctggtcac catccggtta ttggtcggga aagcgggcgc
    33421 ttcgaaaata ccggttatcg cacgcagacc aattaatgac attaatcccg tggcaaagcc
    33481 ctggaataaa gtcgccactg accaaccaaa tatcgcaata aaataagtca cgcgagaacc
    33541 tacgcgatct aaaaaccaac cgccggggat ctggcatagc gtataaagcc aggcgaaggc
    33601 cgaaaataca tagcccattt ccgctttcgt aatgccgaac tcttcctgaa tatgggcgga
    33661 agccacggcc aggttagcgc ggtcaacata acaaatgacc accgtaataa agatcatcac
    33721 cagcgtcaga taacgccgac gccccggctt tgctgcatta acgggaatat ccatagcgag
    33781 ctttctccag attttgggca tagcgaagcc gctcaccatg ccctgtaatt tacagagggt
    33841 tatatttttg tattgctgtt ttagtgcccg atgaggggct tacgtggcag gaattaccac
    33901 tctgctacgc tgttatcttc atgacgccag agcggattac gccagtccgg ggcattttta
    33961 ctgaactcaa taactttagc ttcgtcgatt tccacaccta agcccggttt cgttaacggt
    34021 ttaaagaagc cgccaaccat gctgaagtct tctttgtttt tcacaaagtc gagtaactcc
    34081 gcgcctttgt tgtaatgaat gcccatactt tgttactgaa gtaccgcgtt ataggaaaca
    34141 aagtcgatat gcaggcaagc cgccagtgca atcggtccga acggacagtg cggcgcaagg
    34201 gtcacatcat aggcttctgc cattccagca attttgtagc attcggtaat accgcctgca
    34261 tgggagagat ccggttgcag aatcgaaata ccgcctgcct ccagcacgcg tttaaaatcg
    34321 aaacgtgaga acatgcgctc acccgccgcc agtggaatat gcgtttgtgc cgccaatttc
    34381 gggtagtatt cggcttgttc cgccagcacc ggctcttcaa taaacagcgg gcgatacggc
    34441 tccagttctt taatcagcac tttcgccatt ggcgcgctga cgcgaccgtg gaaatcaaga
    34501 ccaaactcaa tctgattgcc aaaagcttca cgaatttgtg ccacggtgtt gactgccgca
    34561 tctaccgcgc gggagttatc aattagcccc agttcttcac aaccgttcag tttgaaggta
    34621 tcgaagccga tttcgcgcag ggttttaatg ccgtcgataa catccgccgg acgatcgccg
    34681 ccgacccaac tgtaggcttt aattttgtcg cgaaccaggc cgcccatcag ttgccagact
    34741 ggcgcattca gcactttgcc tttgatatcc cataacgcct ggtcgatccc ggcgatggcg
    34801 ctcatcagga ttggaccgcc acgataaaat ccggcgcgat acatcacttg ccataagtcg
    34861 ttgatgcgcg aaggatcctg accaatcaaa tagtcaccca gctcgtgaac tgccgcttcc
    34921 accgtacggg cgcggccttc aatcacgggc tcgccccaac cgaccacgcc ttcatcggtt
    34981 tcaattttca ggaacatcca gcggggaggt aaacgatacg tggtaatttt ggtgattttc
    35041 attgcactgc ctctcgatac gccttaacaa atgctgctgc ctgctgcgcg gtgcgttcta
    35101 cggactgccc ggcgcgataa agatcgctgc ctaagcccgc cccaacacaa cctgcgtcta
    35161 tccactgcgc caggttttct ggcgtcacgc cgccaacggc aaagactgcg atgtccggtg
    35221 gtaataccac ttttaacgct ttgatgtatt gcggaccaaa agccgatgac ggaaatattt
    35281 tcagcgcctg cgcgcccgct tcgagcgcgg taaaggcttc ggtcgccgtc gcgcagccgg
    35341 ggcaaacggt catgccgtag cccaccgcgc ggcggatcac ttcactatgg atattgggcg
    35401 taacgatgag ctgacagccc atcctggcga gcgcatcgac ctgttcaggt ttcagtaccg
    35461 tacctgcgcc aatcaacgcc ttgtcgccat acgcatcaac gatggcggga atgctctgct
    35521 cccattgtgg ggaattcagc gggatttcaa ccgcgtcgaa cccggcgtca atcaccgcgc
    35581 caacatgcgc cagcgcctcg tcgggcgtaa tgccgcgcaa aatggcgatc agcgggagtt
    35641 tagtttgcca ctgcatgagc gatgctcctt ataccagcct gaaatgccgt gtcgcccgcc
    35701 accgccgcca cgtcgcaacc catcgcctga aaggcttgct ggtagcgcgc ggtcagcgat
    35761 gttccggcga caagggtgat ggcgtgttga tgggtcacat agtcgcgcat actggcaacc
    35821 tctgcgccaa tcaacaaacc agagagaaat tcgctgacct gttcgcggag aagtgttccc
    35881 agcacatgcg aggcgcgaac ttcaaaaagc tgcggcaata tggcgggcgc attaaggccg
    35941 cgctcaaggc cagccgcgaa ggcatcggca gagttttcct gcggcggcaa acctgcgcca
    36001 atcaatgagt gatttaacag taaatgatgt aattcaccgg tcatcacggt gcgaaaatcg
    36061 ttgatttgct ggctatcggc ctgcacccat ttgcaatgcg taccgggcat aacataaaga
    36121 gaggaaggag ccagagctcg cgcgccgatc aattgtgttt cttcgccgcg catcacattg
    36181 tggttatcgt catgagagac gcataatccg ggaataatcc agatattgtc gccaacagac
    36241 gttaattgtt cgccaataga cgaaaaacgg gcaggaacag ataaatacgg agcaactttc
    36301 cagccgacat tgctgccgac cattcctgcc atcaccactg gcgtattttc ttcacgccag
    36361 tcggtcgtga cttctgctaa caccgcagcc ggagattttc cgttcaggcg cgtgacgcct
    36421 gcttctgatt gcctgctctc caggcagtgg tcgccctgat aaagccaggc gcgcagattg
    36481 gtcgatcccc agtcaattgc gatgtagcga gctgtcatgt gatttccttt aaccttcgtg
    36541 tcgagctggc aatcatggta agcgccgcct gctctgccgc atcgccgtcc tgatgccgta
    36601 tcgcatcgaa cagcgcctta tgttcctgga gcgtttgcgg catgttggcc tcatcgccca
    36661 tccaggttcg ttcaaaaacc gcccgctgca gcgaactgat cgcaatgcta agttgctgta
    36721 acaccgggtt atgcaccgac tgcagcaccg cctcgtggta gcgaatatcc gcttcgttaa
    36781 acgcttcgcg gtcctgattg ttggcaatca tctcgttcag cgccgattca atctgcgcca
    36841 gatcgctgga ggtcgcgcgc tctgctgccc aacgggcaat cgccggttcc accagatttc
    36901 gcacttcgct catggcactg ataagccgtg ggtcgtagtc gttttccagc acccattgca
    36961 gtacgtcagt gtcgaggtaa ttccactggt tacgcggtgc cacaaacgcc ccgcgataac
    37021 gtttcatttc aatcagccgc ttcgccatca gcgaacggaa cacctcacgg atgatgttgc
    37081 gcgaggttgc aaactcttca cagagctccg cctcagccgg aagtggcgag ccaggtacgt
    37141 atttgccgtg aacgatctgt ttacccagcg taatgacaat gcgatcggtt ttattgagag
    37201 tcatggagag tccttgtgct ctgtatgttc ttctctactt taccccgatc gatgcataac
    37261 gcggcaactt tgtagtacca gcgtgatgac gttcgcgttt gccgtgcgtg taatgtagta
    37321 caaacttata ttgttgtgct acaatttaga tcaaaaaaag aacaatgtat aaaaaaatga
    37381 catgcgtcgg gcagaaatct gaaaagggat atcaggcgct aaacaggagg gaaagaagag
    37441 tatgctttca acggcttagc tactcgttta aaagattaat catgaagttg aattttaagg
    37501 gattttttaa ggctgccggt ttattcccac tggcgctgat gctttcaggc tgtatctcgt
    37561 atgctctggt ttcccatacc gcaaagggta gttcaggaaa gtatcaatcg cagtcagaca
    37621 ccatcactgg gctatcgcag gcaaaagata gtaatggaac aaaaggctat gtttttgtag
    37681 gggaatcgct ggattacctt atcactgatg gtgccgatga catcgttaag atgctcaatg
    37741 atccagcact taaccggcac aatattcagg ttgccgatga cgcaagattt gttttaaatg
    37801 cggggaaaaa gaaatttacc ggcacaatat cgctttacta ctactggaat aacgaagaag
    37861 aaaaggcact ggcaacgcat tatggttttg cctgtggtgt tcaacactgt accaggtcac
    37921 tggaaaacct aaaaggcaca atccatgaga aaaataaaaa catggattac tcaaaggtga
    37981 tggcgttcta ccatccattt aaagtgcgat tttatgaata ctattcaccc agaggcattc
    38041 cggatggtgt ttccgcagca ttactgccag tgactgttac gctggacatc attactgcac
    38101 cgctgcaatt tctggttgta tatgcagtaa accaataatc agtaagcggg caaacgcgtt
    38161 tatgctgttt gcccgcccac agattaattc agcacatact tctcaatagc aaacgccacg
    38221 ccatcttcaa ggttggattt tgtgacaaag ttcgccattt ctttcaccga aggaatagcg
    38281 ttatccatcg ccacaccgac gcctgcatat tcaatcattg cgatgtcgtt ttcctgatcg
    38341 ccaatcgcca tgatttcttc cggtttaata cctaacacgt cggccagtga tttcacaccc
    38401 gtacctttgt taacgcgttt atcgaggatt tcgaggaagt acggcgcact tttcagcacg
    38461 gtatatttct ctttcacttc ctgcggaata cgcgcgatag cctggtcgag gatggcgggt
    38521 tcatcaatca tcatcacttt caggaactgg gtattggggt ccattttctc cgcttcgcag
    38581 aacaccagcg gaattgtggc aacgaaggat tcatgcaccg tgtagtagct gatatcgcgg
    38641 ttagcggtgt acagcgtggt gcggtccagg gcgtggaaat gagaaccgac ttcgcgagag
    38701 agtttttcca ggaaacgata gtcgtcatag ctgagagcgg tttgcgccac ggtgctacca
    38761 tcagcggcct tctgtaccag cgcgccgtta taagtaatgc agtagtcgcc cggctgttcc
    38821 atatgcagct ctttcaggta gttgtgcaca cctgcatacg gacgacccgt cgttagcacg
    38881 acattcacgc cacgggcgcg agctgcggca atcgcatttt taacggcggg tgaaatggtg
    38941 tgatcgggca gcagaagggt gccatccata tcgatagcaa tgagtttgat agccatgagt
    39001 tcccaaggta gattagttcc tgacccatgc taacgcgatt ccgctcaaaa atcagtacaa
    39061 cacccgaggg aaaaggggat gcaacgcggg ttgcatgctc cccatttgct tagcggaaga
    39121 gtttcccttt cagcagttcc atgcctgcgg aaagcagatc gttattggct tgtgctgaca
    39181 cttcaccttg cggtgagagc gcatcaataa tcttcggcaa ttgctctgcc agtaaactgg
    39241 aagccgtact ggtatccacg ccaagttttt gcccgagatc ggacaccgca tttgtgccga
    39301 gcgccgattc cagttgctcg ccgctaactg attgatttct ctgttgatta ctcaaccaag
    39361 ttgagagaat ggcccctaag ccgccacttt gcagtttttc cagcagcacc tgaatgccac
    39421 cctgctcctc aacccaactt aaaatcgcct gatatttccc cgcatcgcct ttcagaaagg
    39481 caccgacaac ttcatcaaaa agccccatga taatcacctg taaagcgtta cgtgttgacc
    39541 caaaaagtat agatctgcgg gatgataatt gcggattgca gaaataaaaa gggcggagat
    39601 gatctccgcc cttttcttat agcttcttgc cggatgcggc gtgaacgcct tatccggcct
    39661 acaaaatcat gcaaaatcaa tatattgcag gagtctcgta ggactaataa gcgtagcgca
    39721 tcaggcacgc tcgcatggtt aacgccatta aatatcaata ttcgccgctt tcagggcgtt
    39781 ctcttcaata aacgcacgac gcggttctac ggcgtcgccc atcagcgtgg tgaacaactg
    39841 gtcagcggca atcgcatcct taacggtaac gcgcagcata cgacggcttt ccgggtccat
    39901 ggtggtttcc cacagctgtt ccgggttcat ctcgcccaga cctttataac gctggatgga
    39961 gaggccacga cgggactctt tcaccagcca gtccagcgcc tgctcgaagc tggctaccgg
    40021 ctgacgacgc tcgccacgtt cgataaacgc atcttcttcc agcaagccac gcagtttctc
    40081 acccagcgtg cagatacgac gatattcgcc accggtgata aactcgtgat ccagcggata
    40141 gtcagtatcc acaccgtggg tacgcacgcg aacaatcggc tcgaacaggt tttgttcggc
    40201 attggtgtgg acatcaaact tccactggct gccgtgctgt tctttgtcgt tcagttcgct
    40261 gaccagcgcg ttcacccagc gggtaacggt ctgctcatca gaaaggtcag cttccgtcaa
    40321 cgtcggctga tagataagct ctttcagcat tgctttcgga taacgacgct ccatgcggtt
    40381 gatcattttc tgcgtcgcgt tgtactcaga taccagtttc tctaacgctt cgccagccaa
    40441 tgccggtgca ctggcgttgg tgtgcagcgt tgcgccgtcc agcgcgatag agatctggta
    40501 ctgatccatc gcttcgtcgt ctttaatgta ctgttcctgc ttgcctttct tcactttgta
    40561 cagcggcggc tgagcgatgt agacgtgacc gcgttcaacg atttccggca tctgacgata
    40621 gaagaaggtc aacagcagcg tacgaatgtg cgagccgtcg acgtccgcat cggtcatgat
    40681 gatgatgctg tgataacgca gtttgtccgg gttgtactcg tcacgaccga taccacagcc
    40741 aagcgcggtg ataagcgtcg ccacttcctg agaagagagc atcttatcga agcgcgcttt
    40801 ctcgacgtta aggattttac ccttcagcgg cagaatcgcc tggttcttgc ggttacgccc
    40861 ctgcttcgca gagccgcccg cggagtcccc ttccaccagg tacagttcgg aaagcgccgg
    40921 atcgcgttcc tggcagtctg ccagtttgcc cggcaggcca gctaagtcga gcgcaccttt
    40981 acggcgggtc atttcacgcg cgcgacgcgc cgcttcacgg gcacgggcag catcgataat
    41041 tttgccaacc acgattttcg catcagttgg gttttccagc aggtattccg ccagcagttc
    41101 gttcatctgc tgttcaaccg ccgatttcac ctcagaagaa accagtttgt ctttggtctg
    41161 ggaggagaat ttcgggtccg gcacttttac ggaaacgacc gcaatcaggc cttcacgcgc
    41221 atcgtcaccg gtggcgctga ctttggcttt tttgctgtag ccttctttgt ccatgtaggc
    41281 attcagggta cgggtcatcg ccgcacggaa gcctgccagg tgagtaccgc cgtcacgctg
    41341 cggaatgttg ttggtaaagc agtagatgtt ttcctggaag ccatcgttcc actgcagcgc
    41401 cacttcaacg ccaataccgt ctttttcggt ggagaagtag aagatatttg ggtggatcgg
    41461 cgttttgttc ttgttcagat attcaacgaa cgctttgatg ccgccttcat agtggaagtg
    41521 gtcttctttg ccgtcacgct tgtcgcgcag acgaatggaa acgccggagt tgaggaacga
    41581 caactcacgc agacgtttcg ccagaatttc atattcgaac tcggtcacat tggtgaaggt
    41641 ttcgaggctg ggccagaaac gcaccatggt gccggttttt tcagtctcgc cggtaaccgc
    41701 cagcggagcc tgcggtacac cgtgttcgta gatctgacgg tgaattttac cctcgcgctg
    41761 aataaccagc tccagttttt gcgacagggc gtttactacc gaaacaccaa cgccgtgcag
    41821 accgccggac actttatagg agttatcgtc aaatttaccg cctgcgtgca gaacggtcat
    41881 gatcacttcc gccgccgata cgccctcttc cgggtgaata ccggtcggaa tgccgcgccc
    41941 gtcatcctgt acagagacag agttatcggc gtgaatggtg acgataattt ctttacagtg
    42001 acccgcgagc gcttcgtcga tagcgttatc taccacctcg aataccatgt ggtgcagacc
    42061 ggtgccgtca tccgtgtcgc cgatatacat acccgggcgc ttacgcaccg catccagccc
    42121 tttcaggact ttgatactgg aggagtcata agaattcgac atcaacgttt ctcgctcatt
    42181 tatacttggg ttaatccgtt attttaccct tttccacggt aaacatcttc gaattttcgt
    42241 ccgacatgtc tataacgtgt tcagcactga tcgcgctgac aaagacctgt gattgcgtcg
    42301 cttttaagcg gctggcaagc aacccgcgac gctcatcatc aagctcagag gcaaaatcat
    42361 ctatcaggta gagacaccgc cgcccgcttt cacgagtgag gaactctcct tgcgccagac
    42421 gtaaggcgca catcaacagc ttaagctgcc cacgcgataa ggtatcttcc accggcgcac
    42481 cgtcggcgcg aatgcgtaag tccgctttat gcgggccatg cgcggtgtag gttagctggc
    42541 gatcgcgttc aaaattacgt tccagcacct cagcgtactc tgtctctttc tcccagccgc
    42601 gctggaaaga gaaagtcaga gaaaactcag ggagaaattg cttacaggta tcatccatat
    42661 cggccgcgat accggcgcta tactccgcgc gccaggtgct gatttgctcc gccagcggga
    42721 tcagttcttt atcccacggg cgtagctgtt cgtaacgagt cacctggcgc agcgccgcat
    42781 tgcgctgctt gagcaatcgc ttgagattgc tccaggcggt gaaaaatcta ggttcgttgt
    42841 gaaagcatcc ccagtcgagg aatgctcttc tgtatttggg gccgccgttg agtaaagtaa
    42901 acccttctgg cgttatcaac tgcatcggca tcaggtgcgc cagttccgcg actttatgcc
    42961 cgtccgtacc gtcaatacgg actttgctgt cgccctgttt gtctttggtt aagccaatcg
    43021 ccgtctcgcg ctcttcgccc tgtaatcgcc cgtggagaac aaatgcctcc tgctcatggc
    43081 gaatgacgcg accaatctgc aaactgcgaa acgcccgacc atggccgagc gtatagatgg
    43141 cttccagcac gctggttttg ccactgccgt tggcacctac cagaaagtta aagccgggag
    43201 ataaagcgag atccgcggtt tcaatattgc ggaaatcgcg gatcaacaag cgggtgaggg
    43261 acattacagt ctcattggca tgacaacata ggcagccgac tgtgatgcgg catcttcaat
    43321 ctgcacgctg gaaaccgaat cggtcagcat catgcggacg ttttcgcatt tcagcgcgtt
    43381 cagaacatcc agcacatagc tgacgttgaa gccgatttcc atctccgcac cgctataggt
    43441 aacgtcgagg atctcttccg cttcttcctg ttccgggttg ttggcagtaa ttttcagctg
    43501 gttttcgctg acatacaggc gcacaccgcg gaatttctcg ttagagagaa tcgccgcgcg
    43561 agcaaacgcc tgcttgagca gatcgcagcc tgcttccaga tgtttgtccg ggttcttcgg
    43621 cagaacgcga cggtaatccg ggaagcgacc atccaccagt ttcgaggtga aaataaagtc
    43681 gccaacgtgg gcacgaatgt tattgctgcc aatctgcaca cgcagcagat tgtcgccgcc
    43741 gtcgagcata cgcatcagtt caatcacgcc tttacgcggt acgatcaccg aatggcttgg
    43801 caaagattga ccaattggca ttgaacagac cgccagacgg tggccgtcgg ttgccacggt
    43861 gcgcagttct tcaccttcgg tttcaaacag cataccgttt aagtaatagc gaacatcctg
    43921 atgcgccatc gaaaactggg ttgcttcaat cagacgcttc atcgtcgcct gcggcagggt
    43981 aaattcgact tcgctctgcc agtcatcgag gttcgggaaa tccgccgctg gcagggtaga
    44041 cagcgaaaaa cggctacgcc cggagcgcac cagcatccgt tcaccttcca gctgcacggc
    44101 aatttccgcg ccttcaggca gaccacggca gatatcaaag aatttgcgcg ccggaacggt
    44161 cgtcgctcct ggctcgtgtg gctgaaccag cgcaacgcgt gccaccattt ccatctcgag
    44221 atcagtaccg gtcagcgaca acgtaccgtc agcaacctgt aacagcagat taccgagaat
    44281 cggtagcgta ggacgaccac ctaacggacc gctcacctgt tgtagcggtt ttaataaatg
    44341 ctcacgttct acggtaaatt tcataggttt acgatgacaa tgttctgatt aaatttgaaa
    44401 aatcttcttt gatatcgtgg ctctcttcac gcaactgctc gatcttacgg caggcatgaa
    44461 gcaccgtcgt gtggtcacgc ccaccaaacg catcgccaat ctctggcaga ctgtggttag
    44521 tcagctcttt cgctagcgcc atcgccatct ggcgcggacg cgccaccgag cgggatcgac
    44581 gcttggaaag gagatccgcg actttgatct tgtagtactc cgccaccgtc ttctgaatat
    44641 tgtcgatggt gaccagtttt tcctgcaatg ccagcaagtc gcgcagcgcc tcacgcacga
    44701 agtcgatggt gatcgcccgt ccggtaaagt tggcgttagc aataacgcgg ttcagcgccc
    44761 cttccagctc acgtacgtta gatcgtagac gcttggcgat aaagaacgcc acttcacccg
    44821 gcaaacgaat gtcattttcg tcggcctttt tcatcaggat cgccacacgg gtttccagct
    44881 ctggcggttc gatcgccaca gtcagtcccc aaccgaagcg ggatttcaaa cgatcctcaa
    44941 cgccgttgat ctctttcgga tagcgatccg aggtgagaat gatctgttga ttaccttcca
    45001 gcagggcgtt gaaggtgtgg aaaaactctt cctgagatcg ttctttatta gcaaaaaact
    45061 gaatatcgtc gatcagcagt gcatctacgg aacggtagta gcgtttaaac tcttcgatcg
    45121 cgttgttttg cagggcttta accatgtcct gaacaaagcg ctcggagtgc atataaacca
    45181 ctttggcatt cggcttgcgc gccataatgc cgttacccac cgcatgcagc aggtgagttt
    45241 tacccagacc cgtgccgcca taaaggaaca acgggttata ggcaccgcca gggttatccg
    45301 ccacctggcg agccgccgcg cgcgccagtt ggttagattt accttcaacg aagttatcaa
    45361 acgtgtgttt gacgtttacg ttagaacgat aggtcggttc tgccggggcc gggacgttat
    45421 cccaacctga gcgcgtagaa ggcgcagcac gttgcggctg cgtttgcgcc acctgtgcag
    45481 gggccgcgac gttgctcgtc actgccgctt gtggcgtttg cgtcaccggt ttggtgccga
    45541 cttcaaaacg cagctgtggg gcatccgctc cgcagaaact ggttagcagt ccattgatat
    45601 tattaaggta cttgtcccgt acccaatcga ggacaaaacg gtttggcgcg tacagggaca
    45661 gcgtgttatc gctcagttcc gcctgcaatg ggcgtatcca catactgaat tctgtggctg
    45721 gtaactcatc ctgcaatcgg gcaagacact gctgccaaag cgaaagtgac acggcggact
    45781 ccactcgaac aaaagtcgat aatgactaag gctgaaacat tcatgattgt tgacgtacgt
    45841 cgaaaagacc ctgcttgagg gtgacgcacg aaccgctgtc tgcggttata tgcccgatca
    45901 agatcctgca aaacgatcgg gaccgcggat catagcctaa actgcgcaag agatcttctg
    45961 tttctcacag attttcccta tttatccaca ggactttcca gaactcgcta agtgtaaacg
    46021 atcctgccgc gaggcgggca cgatttacgc cgcatattgg aaaatttaat gaccatagac
    46081 aaaaattggc ttaatcgatc taataaagat ccaggacgat ccttgcgctt tacccatcag
    46141 cccgtataat cctccacccg gcgcgccatg ctggtttcca ctggtgtgag gccgtacatt
    46201 ttccctgcga aaaggtgcgg aaaagcgcgg taaataagga aagagaattg actccggtgt
    46261 gtacaattat tacaatccgg cctctttaat cacccatggc ttcggtgtcc atcgtttcat
    46321 ttttcggcgg atatccaata aagccattga atttattcaa gtttaggtag aaatcgccat
    46381 gaaacgcact tttcaaccgt ctgtactgaa gcgcaaccgt tctcacggct tccgtgctcg
    46441 tatggctact aaaaatggtc gtcaggttct ggcacgtcgt cgtgctaaag gccgcgctcg
    46501 tctgaccgtt tctaagtaat aaagctaacc cctgagtggt taagctcgca tttcccaggg
    46561 agttacgctt gttaactccc agtcaattca cattcgtctt ccagcagcca caacgggctg
    46621 gcacgccgca aattaccatt ctcggccgcc tgaattcgct ggggcatccc cgtatcggtc
    46681 ttacagtcgc caagaaaaac gttcgacgcg cccatgaacg caatcggatt aaacgtctga
    46741 cgcgtgaaag cttccgtctg cgccaacatg aactcccggc tatggatttc gtggtggtgg
    46801 cgaaaaaagg ggttgccgac ctcgataacc gtgctctctc ggaagcgttg gaaaaattat
    46861 ggcgccgcca ctgtctcctg gctcgcgggt cctgatagcc ctcattcggg tctatcaacg
    46921 cctgattagt ccgctactcg ggccgcattg tcgtttcact ccaacctgtt caagctacgg
    46981 aattgaggca ttgcgcaggt ttggagtgat aaaaggcagt tggttgacgg tgaaacgcgt
    47041 attaaaatgc caccctttac accctggtgg tgacgatccc gtcccgcccg gaccatttga
    47101 taccagagaa cactaacgat ggattcgcaa cgcaatcttt tagtcatcgc tttgctgttc
    47161 gtgtctttca tgatctggca agcctgggag caggataaaa acccgcaacc tcaggcccaa
    47221 cagaccacgc agacaacgac caccgcagcg ggtagcgccg ccgaccaggg cgtaccggcc
    47281 agtggccagg ggaaactgat ctcggttaag accgacgtgc ttgatctgac catcaacacc
    47341 cgtggtggtg atgttgagca agctctgctg cctgcttacc cgaaagagct gaactctacc
    47401 cagccgttcc agctgttgga aacttcaccg cagtttattt atcaggcaca gagcggtctg
    47461 accggtcgtg atggcccgga taacccggct aacggcccgc gtccgctgta taacgttgaa
    47521 aaagacgctt atgtgctggc tgaaggtcaa aacgaactgc aggtgccgat gacgtatacc
    47581 gacgcggcag gcaacacgtt taccaaaacg tttgtcctga aacgtggtga ttacgctgtc
    47641 aacgtcaact acaacgtgca gaacgctggc gagaaaccgc tggaaatctc ctcgtttggt
    47701 cagttgaagc aatccatcac tctgccaccg catctcgata ccggaagcag caacttcgca
    47761 ctgcacacct tccgtggcgc ggcgtactcc acgcctgacg agaagtatga gaaatacaag
    47821 ttcgatacca ttgccgataa cgaaaacctg aacatctctt cgaaaggtgg ttgggtggcg
    47881 atgctgcaac agtatttcgc gacggcgtgg atcccgcata acgacggtac caacaacttc
    47941 tataccgcta atctgggtaa cggcatcgtc gctatcggct ataaatctca gccggtactg
    48001 gttcagcctg gtcagactgg cgcgatgaac agcaccctgt gggttggccc ggaaatccag
    48061 gacaaaatgg cagctgttgc tccgcacctg gatctgaccg ttgattacgg ttggttgtgg
    48121 ttcatctctc agccgctgtt caaactgctg aaatggatcc atagctttgt gggtaactgg
    48181 ggcttctcca ttatcatcat cacctttatc gttcgtggca tcatgtaccc gctgaccaaa
    48241 gcgcagtaca cctccatggc gaagatgcgt atgctgcagc cgaagattca ggcaatgcgt
    48301 gagcgtctgg gcgatgacaa acagcgtatc agtcaggaaa tgatggcgct gtacaaagct
    48361 gagaaggtta acccgctggg cggctgcttc ccgctgctga tccagatgcc aatcttcctg
    48421 gcgttgtact acatgctgat gggttccgtt gaactgcgtc aggcaccgtt tgcactgtgg
    48481 atccacgacc tgtcggcaca ggacccgtac tacatcctgc cgatcctgat gggcgtaacg
    48541 atgttcttca ttcagaagat gtcgccgacc actgtgaccg acccgatgca gcagaagatc
    48601 atgaccttta tgccggtcat cttcaccgtg ttcttcctgt ggttcccgtc aggtctggta
    48661 ctgtactata tcgtcagcaa cctggtaacc attattcagc agcagctgat ttaccgtggt
    48721 ctggaaaaac gtggcctgca tagccgcgag aagaaaaaat cctgattcgg tgagttttcg
    48781 ctaaaataag ggcggtcagt tgaccgcctt ttttcttttc gtagggcgga taagcaccgc
    48841 gcatccgcca cacaaagcaa caggaacatc atgagcgata atgacactat cgtagcccag
    48901 gccacacctc cgggacgtgg cggcgttggc atcctgcgca tctccggctt caaagcccgt
    48961 gaagttgccg aaaccgtgct gggtaaactg cctaagccgc gctacgccga ttatcttccg
    49021 tttaaagacg ccgacggcag cgtgctcgat caggggattg cgctatggtt ccctggcccg
    49081 aactcgttca ccggcgaaga tgtgctggaa ctgcaaggtc atggcggtcc ggtgatcctc
    49141 gacctgctgt taaaacgcat tctgaccatt cccggcctgc ggattgctcg ccctggtgag
    49201 ttttccgaac gcgcgtttct taacgataaa cttgacttag cccaggccga ggcgattgcc
    49261 gatcttatcg acgccagttc ggaacaggcg gcccgttcgg cacttaactc gctgcaaggc
    49321 gcattctccg cacgggttaa tcatctggta gaagccctca cccacttgcg catttacgtc
    49381 gaagcggcaa ttgatttccc tgatgaagag atcgatttcc tctccgacgg caaaattgaa
    49441 gcccagctta atgacgtgat ggccgatctc gatgcagtgc gtgctgaagc acgtcagggt
    49501 agtttgttgc gcgaagggat gaaagtagtg attgccggac gtcctaatgc cggtaaatcg
    49561 agcctgttaa acgcgctggc gggccgtgaa gcggcaatcg taaccgatat cgccggaacc
    49621 acgcgtgacg tgctgcgtga gcatatccac attgacggaa tgccgctgca tatcatcgac
    49681 actgccgggc tacgtgaggc cagtgacgaa gtggaacgta ttggtatcga gcgcgcgtgg
    49741 caggaaattg aacaggccga ccgcgtgctg tttatggtcg atggcaccac gacagacgcc
    49801 gttgatccgg cagagatctg gccggaattt atcgcccgtc tgcccgcgaa actgccgatc
    49861 accgtggtac gcaataaagc cgatatcacc ggcgaaacgc tgggaatgag tgaagtgaac
    49921 ggtcacgcgt taattcgtct ctcggcaagg actggtgaag gcgtggacgt gctgcgtaac
    49981 catctcaaac agagcatggg ctttgacacc aatatggaag gcggcttcct ggcgcgccgt
    50041 cgccacttac aggcgctgga acaggcagcg gagcatttgc agcaaggtaa agcgcaactg
    50101 ttgggagcct gggcaggtga actgctggcg gaagagttgc gcctggcaca gcagaactta
    50161 agcgaaatca ccggggaatt tacttcagac gacctgctg
//
LOCUS       DAJVQH010000015        43319 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJVQH010000015 DAJVQH010000000
VERSION     DAJVQH010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.015"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..621)
                     /locus_tag="OQM87_000987"
     CDS             complement(<1..621)
                     /locus_tag="OQM87_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1507218.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGT"
     gene            complement(761..1948)
                     /gene="metC"
                     /locus_tag="OQM87_000988"
     CDS             complement(761..1948)
                     /gene="metC"
                     /locus_tag="OQM87_000988"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCS1507219.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            2200..2934
                     /gene="exbB"
                     /locus_tag="OQM87_000989"
     CDS             2200..2934
                     /gene="exbB"
                     /locus_tag="OQM87_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1507220.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            2941..3366
                     /gene="exbD"
                     /locus_tag="OQM87_000990"
     CDS             2941..3366
                     /gene="exbD"
                     /locus_tag="OQM87_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCS1507221.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3638..4523)
                     /gene="yghA"
                     /locus_tag="OQM87_000991"
                     /pseudo
     CDS             complement(3638..4523)
                     /gene="yghA"
                     /locus_tag="OQM87_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
     gene            4714..5208
                     /locus_tag="OQM87_000992"
     CDS             4714..5208
                     /locus_tag="OQM87_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCS1507222.1"
                     /translation="MERFFENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            complement(5248..6288)
                     /gene="gpr"
                     /locus_tag="OQM87_000993"
     CDS             complement(5248..6288)
                     /gene="gpr"
                     /locus_tag="OQM87_000993"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCS1507223.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            6445..7332
                     /locus_tag="OQM87_000994"
     CDS             6445..7332
                     /locus_tag="OQM87_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1507224.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            7451..7738
                     /locus_tag="OQM87_000995"
     CDS             7451..7738
                     /locus_tag="OQM87_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCS1507225.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            7927..9045
                     /gene="hybO"
                     /locus_tag="OQM87_000996"
     CDS             7927..9045
                     /gene="hybO"
                     /locus_tag="OQM87_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCS1507226.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            9048..10034
                     /gene="hybA"
                     /locus_tag="OQM87_000997"
     CDS             9048..10034
                     /gene="hybA"
                     /locus_tag="OQM87_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCS1507227.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            10024..11202
                     /gene="hybB"
                     /locus_tag="OQM87_000998"
     CDS             10024..11202
                     /gene="hybB"
                     /locus_tag="OQM87_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCS1507228.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            11199..12902
                     /gene="hybC"
                     /locus_tag="OQM87_000999"
     CDS             11199..12902
                     /gene="hybC"
                     /locus_tag="OQM87_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCS1507229.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            12902..13396
                     /locus_tag="OQM87_001000"
     CDS             12902..13396
                     /locus_tag="OQM87_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCS1507230.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLSDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            13389..13877
                     /gene="hybE"
                     /locus_tag="OQM87_001001"
     CDS             13389..13877
                     /gene="hybE"
                     /locus_tag="OQM87_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCS1507231.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            13870..14211
                     /gene="hypA"
                     /locus_tag="OQM87_001002"
     CDS             13870..14211
                     /gene="hypA"
                     /locus_tag="OQM87_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1507232.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            14345..14593
                     /gene="hybG"
                     /locus_tag="OQM87_001003"
     CDS             14345..14593
                     /gene="hybG"
                     /locus_tag="OQM87_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCS1507233.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(14716..15582)
                     /gene="yghU"
                     /locus_tag="OQM87_001004"
     CDS             complement(14716..15582)
                     /gene="yghU"
                     /locus_tag="OQM87_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCS1507234.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            15787..17646
                     /gene="gss"
                     /locus_tag="OQM87_001005"
     CDS             15787..17646
                     /gene="gss"
                     /locus_tag="OQM87_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCS1507235.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            17938..19437
                     /gene="pitB"
                     /locus_tag="OQM87_001006"
     CDS             17938..19437
                     /gene="pitB"
                     /locus_tag="OQM87_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCS1507236.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(19486..20178)
                     /locus_tag="OQM87_001007"
     CDS             complement(19486..20178)
                     /locus_tag="OQM87_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS1507237.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            20352..21065
                     /locus_tag="OQM87_001008"
     CDS             20352..21065
                     /locus_tag="OQM87_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCS1507238.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            21097..21855
                     /locus_tag="OQM87_001009"
     CDS             21097..21855
                     /locus_tag="OQM87_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507239.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            21901..23250
                     /locus_tag="OQM87_001010"
     CDS             21901..23250
                     /locus_tag="OQM87_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCS1507240.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            23250..24074
                     /locus_tag="OQM87_001011"
     CDS             23250..24074
                     /locus_tag="OQM87_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507241.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            24086..24646
                     /locus_tag="OQM87_001012"
     CDS             24086..24646
                     /locus_tag="OQM87_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1507242.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLSNE"
     gene            24677..25747
                     /gene="lptF"
                     /locus_tag="OQM87_001013"
     CDS             24677..25747
                     /gene="lptF"
                     /locus_tag="OQM87_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1507243.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRTWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPWQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            25744..26823
                     /gene="lptG"
                     /locus_tag="OQM87_001014"
     CDS             25744..26823
                     /gene="lptG"
                     /locus_tag="OQM87_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1507244.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(26859..28031)
                     /locus_tag="OQM87_001015"
     CDS             complement(26859..28031)
                     /locus_tag="OQM87_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1507245.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(28031..28279)
                     /locus_tag="OQM87_001016"
     CDS             complement(28031..28279)
                     /locus_tag="OQM87_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1507246.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            complement(28312..29226)
                     /locus_tag="OQM87_001017"
     CDS             complement(28312..29226)
                     /locus_tag="OQM87_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCS1507247.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(29223..30911)
                     /locus_tag="OQM87_001018"
                     /pseudo
     CDS             complement(29223..30911)
                     /locus_tag="OQM87_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            31315..32457
                     /locus_tag="OQM87_001019"
                     /pseudo
     CDS             31315..32457
                     /locus_tag="OQM87_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            complement(32581..33351)
                     /gene="glcC"
                     /locus_tag="OQM87_001020"
     CDS             complement(32581..33351)
                     /gene="glcC"
                     /locus_tag="OQM87_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCS1507248.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            33597..35096
                     /gene="glcD"
                     /locus_tag="OQM87_001021"
     CDS             33597..35096
                     /gene="glcD"
                     /locus_tag="OQM87_001021"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HCS1507249.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANELGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            35096..36148
                     /gene="glcE"
                     /locus_tag="OQM87_001022"
     CDS             35096..36148
                     /gene="glcE"
                     /locus_tag="OQM87_001022"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCS1507250.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFCYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            36159..37382
                     /gene="glcF"
                     /locus_tag="OQM87_001023"
     CDS             36159..37382
                     /gene="glcF"
                     /locus_tag="OQM87_001023"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HCS1507251.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            37387..37791
                     /locus_tag="OQM87_001024"
     CDS             37387..37791
                     /locus_tag="OQM87_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HCS1507252.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            37813..39984
                     /gene="glcB"
                     /locus_tag="OQM87_001025"
     CDS             37813..39984
                     /gene="glcB"
                     /locus_tag="OQM87_001025"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCS1507253.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGIVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            40339..42021
                     /gene="glcA"
                     /locus_tag="OQM87_001026"
     CDS             40339..42021
                     /gene="glcA"
                     /locus_tag="OQM87_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HCS1507254.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFAGVIGVITLLQAYVFTGMLVS"
BASE COUNT        10377 a        10647 c        11534 g        10761 t
ORIGIN      
        1 cgtgcctaac aagccagcgg ttaacaaggc aattggcagg atcatcagga acgtcattac
       61 ctgatcttca tggcgtttaa cgaacggaat catacttaag gcatagccaa aactggttac
      121 cacgctgacc cacagcaatc cacttaacca gttgaaaaac tggaagcggc ggtttggcag
      181 accggaaatt cccgccatgg ttggcagcag cgtacggaca aatgcaagaa aacgtccagc
      241 cagcagcgcc agcagaccgt ggcggtcaaa catgcaggtg gcgcgctggt gatatttagc
      301 aggaagctgt gccagccagc ctttcaccgt tttggtattc cctaaccagc gcccctgaat
      361 ataacttagc cagcagccca gacttgctgc ggcggtcaga atcgcaatcg taggcagaaa
      421 atccataacc ccttgggcaa tcaatgcgcc tgccagtatc aacaagctgt cgcctggcaa
      481 aaatgaggcg ggcagcaggc cgttttctaa aaacagcgtg gcaaacatga caaagtaaac
      541 aacgctaaca atatgaggat ccgccagcgc ggcaaagtcg tgttgccaga gcgcagcgat
      601 gatatcttga ataacagcca tggactttcc tgtggaacag cgtttaagct tttatctgcc
      661 tattgtactc ctgaatcgtc cgggacgcct tgatcccgga cgcaacaatt taagactttt
      721 cacaataaaa tgtctgcaaa attgtccaaa agtggcaatg ttatacaatc cgtgcaaaac
      781 cggcgtccag atcggcaatc agatcgtcga catcttccag accaatatgc aggcgaatca
      841 aggtcccgct aaaatcgatc tcgccttgtg ggcgaatggc ggcgatatgt tctggttgat
      901 ttgccaggat caacgattca tacccgcccc acgagtaggc catgctgaat aaactgaagt
      961 tatccagata gttcgccagc tcttcatcat tgagtttttt cttaagcaca aaggaaaata
     1021 gcccgctgct gcctgtaaag tctcgtttcc agaattcgtg tcctttactg ccaggcaggg
     1081 cagggtggtt aactcgcgca acttgcggat gttctgccag ccattcggcc actttcagac
     1141 tgctttcatg atgttgacgc aaacgcacac ctaatgtgcg caggccacgg ctggttatat
     1201 aggcggtatc ggcatcgacc atctggccca tcagataggc attttcccgt agctgctccc
     1261 agcaacgggc attgcacacg gcagtgccaa tcatcgcatc tgaatgccca accagatatt
     1321 tggtggcggc ctgaatagaa acatcgatgc caaaatccag cgccttaaac agcacaccgg
     1381 ctgcccaggt gttgtcgatc ataatgatgg catccggcac cacactgcgc acggcggcaa
     1441 caatcgccgg aacgtcgtgg acttccatag tgatggaacc cggcgattcc agaaacacga
     1501 ttttagtgtt tggctgcaga tgcttaacga tatcggcacc aatcagcgga tcaaaccacg
     1561 atgtcgttac gcccagtttg ctgaggattt tgctacagaa atcctgactc ggttcatagg
     1621 cggtgttggt catcaacaca tgatcgccct gttcgacaaa agcaagaatc gaattagcaa
     1681 ccgctgccgc cccgcaggga aatagcgcgc agcctgcgcc accttccagt tcacacatcg
     1741 cttgttgtaa ggaaaaatgg gttaacgttc cgcgccgtcc atagaacaac tcgccattgg
     1801 cgcgattgcg cgtcgcgtgc tttttggctt ccatactctc aaataccagc gaagaagcgc
     1861 gctgaatcac gctatttacc gcgccaagag tgtatttttt gctgcgtcct gcattcacca
     1921 gttgagtatc aagctttttg tccgccatgt cgggattcct gtttttatac gtctggatgt
     1981 ctaaactagc atgaatattc gcgggcgcat cctgaagaat tggcataaag cagaaaattt
     2041 tcgcaaatgg cgtgctggtg acttttttac tgcgtaaacg caaggaaaag aaagcaaagg
     2101 tacggcagta tgcaaatagt aatgagaacg actatcaatt cgacgtcgtt ttgatattat
     2161 tatgcgcaga ttttgtgact tgcgtcctgg agatacacag tgggtaataa tttaatgcag
     2221 acggaccttt ccgtctgggg tatgtatcag cacgccgata ttgtcgttaa gtgcgtgatg
     2281 attgggctta ttttggcctc cgtagtcacc tgggcaatct tcttcagtaa gagcgtagag
     2341 ttcttcaatc agaagcgtcg ccttaagcgc gagcagcaac tgctggctga agcacgttcc
     2401 ttaaaccagg ccaacgatat cgccgctgat tttggtagca aaagcttaag cctgcatttg
     2461 ctcaatgaag cgcagaatga gctggaactg tcagaaggca gcgacgataa cgaaggtatt
     2521 aaagaacgta ccagcttccg tctggagcgt cgggtcgccg cagtgggtcg tcaaatgggc
     2581 cgcggtaacg gctatctggc aaccatcggc gcgatttctc cgttcgttgg tctgtttggt
     2641 acggtctggg gcatcatgaa cagctttatt ggtatcgcgc aaacgcagac cactaacctg
     2701 gcagtcgttg cgccgggtat cgcagaagct ctgttagcaa cggcaatcgg cctcgtggca
     2761 gcgattcctg cggtcgttat ctataacgta tttgcacgcc agattggcgg ctttaaagcg
     2821 atgctgggtg atgttgcagc gcaggtattg ttgctgcaaa gccgtgacct ggatctggaa
     2881 gccagcgccg ctgcgcatcc ggttcgtgtc gcacaaaaat tacgcgcagg ataatgtccg
     2941 atggcaatgc atcttaacga aaacctcgac gataacggcg aaatgcatga tatcaacgtg
     3001 acgccgttta tcgacgtgat gttggttctg ctgattatct ttatggtggc ggcaccgtta
     3061 gcgacggtag atgtgaaggt gaacttgcct gcttctacca gcacgccgca gccgcgcccg
     3121 gaaaaaccgg tttatctgtc ggtgaaggca gacaactcga tgtttatcgg taacgatccg
     3181 gtcaccgatg aaacaatgat tacggcgttg aatgcgttaa ccgaaggcaa gaaagacacc
     3241 accatcttct tccgagcgga caaaaccgtc gattacgaga cgttgatgaa ggtaatggat
     3301 acgctgcatc aggcgggtta cctgaagata ggtctggtcg gcgaagaaac cgccaaagcg
     3361 aagtaaagta gaattgcctg atacgctacg ctcatcaggc ctacaaaatc tattgcaaca
     3421 tgttgaatct tcgtgcgttt gtaggccgga taaggcgttc acgacgcatc cggcattagg
     3481 tgctcaatgc ctgatgcgct acgcttatca ggcctacaaa atctattgca acatgttgaa
     3541 tcttcatgcg tttgtaggcg gataaggcgt tttcgccaca tcaggtaaga gtgaattcac
     3601 aatgatgccc ggttgctttt cacaaccggg cattttttta acctaaatgc tcgccgccgc
     3661 acacgccgtg cacttctgcg gtgacgtagc tcgactcctg acttgccaga taaacatata
     3721 caggggccag ttccgccggt tgccccgcac gcttcatcgg cgttttctga ccaaactgcg
     3781 ggatcttatc ctgcgtttgt ccgccggaaa tttgcagtgc cgtccagata gggcctggcg
     3841 cgacaatatt cacccgaata cctttctccg cgacctgttt tgccaggcca cggctgtagt
     3901 tcagaatcgc cgccttcgta gccgcatagt ccagtaaatg cggacttggc tggtatgcct
     3961 ggattgacga agtggtgatg atacttgcac ctttcggtag cagggggatc gcttcctggg
     4021 ttagccagaa cagcgcgaaa acgttaatgg caaaggtctt ttgaaactgt tcgctggtga
     4081 ggtctgcaat atccggaatg gcaacctgtt tcccggcgac cagcgccata atatccagcc
     4141 cgcctaacgc cttgtgcgct tcgtgaacca gcgaacgggc gaatttctca tcgcttaaat
     4201 cgcctggcag cagaacggct ttgcgtccgc attcttcaat gatctttttc acatcctgag
     4261 cgtcttcttc ttccacggga agataactga tcgccacgtc agccccttct cgcgcgtaag
     4321 cgatggcggc agcgcgacct attccggaat cgccccctgt caccagtgct ttacgatctt
     4381 tcaggcgagc gctaccaaca taggttttct cgccgcaatc cggtatcggt gtcatcttcg
     4441 cctggatgcc tggcgtcggt tgtttctgtt tgggatattc accagtgtaa tactgcgtgg
     4501 tcgggtcatt ttaaatgaga catcgttttt ctcccttcag gttcaacgtc ctttaagggt
     4561 agacgctctc gatgcgttga taagggaacc aggaagatcc ctaaacctca gaattatgcg
     4621 acaaaggttt aacgtatatg ttgatttgct gttgcgtgct gtttactcaa ttgcgatata
     4681 ctgttgcccg ttttaactac acgacaggaa tgtatggaac gtttttttga aaatgcaatg
     4741 tatgcttctc gctggctgct tgcccccgtg tactttggcc tttcgctggc gttagttgcc
     4801 ctggcgctga agttcttcca ggagattatt cacgtactgc cgaatatctt ctcgatggcg
     4861 gaatcagatt tgatcctcgt gttgctgtcg ctggtggata tgacgctggt tggcggtttg
     4921 ctggtgatgg tgatgttttc cggttatgag aatttcgtct cacagctgga tatctccgag
     4981 aacaaagaga agctgaactg gctggggaaa atggacgcaa cgtcgctgaa aaacaaagta
     5041 gcagcgtcga ttgtggcaat ttcttccatt cacttactgc gcgtctttat ggatgcgaaa
     5101 aatgtcccgg ataacaaact gatgtggtac gtcattatcc atctgacgtt tgtgctttct
     5161 gcatttgtga tgggctatct tgaccgactg actcgtcata atcactgatc tgattcgggc
     5221 gcggttcgcg cgcccgttat taacaggtca tttatcggac gacgcctgcc acagattcag
     5281 ttcgccatcg gcgatatgct gatcaatctg cgccagctcc tcggtgctaa atgtcagatt
     5341 attcagcgcc tgcacgttct cctctagttg ctccgcgcgg ctggcaccaa tcaataccga
     5401 cgtcacgcga tcatctttca gcaaccagct taacgccatt tgcgccattg attgtccacg
     5461 ctgctgtgcc atttcattca ataagcgcag gctgttgagg ttggcttcgg taagcatttt
     5521 cggcgtcaga ccacgaattt tattcccttc acgatgcatc cgtgaatctt gcggaatgcc
     5581 gttgagatat tttccggtca gcaatccctg agccagagga gtaaaggcaa tacagcccac
     5641 gccgttattt tgcagggtat ccagcaggcc gcttttatcc acccagcggt tcagtaaatt
     5701 gtacgaaggt tgatgaatta aaagcggaat tttccactcg tgcagcaact cgaccatttt
     5761 ttgcgtccgc tctggcgagt aagaggagat cccgacatac agcgccttac cgctttgtac
     5821 cgcatgagcc agcgcagagg cggtttcttc catcggcgta ttttcatcga cgcgatgaga
     5881 gtaaaagata tcgacatact caagccccat acgcttcagg ctttggtcga ggctggcgag
     5941 caggtattta cgtgaaccgc cagagccgta cgggccgggc cacatatcgt agccagcctt
     6001 ggtagagata atcagttcat cgcgataagc agcaaaatcc tcgcgcagca ggcgaccaaa
     6061 gttctcttct gcgcttcctg gaggcggccc gtaattgttg gctaaatcaa agtgcgtaat
     6121 gcctaaatca aacgctttac gcaggattgc acgctgtgat tccagcgcgt taacgtgacc
     6181 gaaattgtgc cataaaccga gcgataacgc gggcaggcgt aaaccacttt ttccgcaata
     6241 gcggtactgc atctgcccgt aacgttcggg attcgctaac cagaccatga cctctccttt
     6301 ccaccgttca atttcgaaac aatgtttcta gtttagcgat tcgccagcgc gtatcccgta
     6361 gtctggctca cagagtgacg aaaaactggg caaaaacacg cgcttatgct ttgcttaaaa
     6421 aaacaccagt tgaggaatgc aacgatgccg cgtttaaccg ccaaagattt cccacaagag
     6481 ttgttggatt actacgacta ttacgttcac gggaaaattt cgaaacgtga gttcctcaac
     6541 cttgcggcga agtatgcggt gggcgggatg acggcattag cgttgtttga tttgctcaag
     6601 ccaaattatg cgctggcgac tcaggtagag tttaccgacc cggagattgt tgctgagtac
     6661 atcacgtatc cttcgccaaa tggtcacggc gaggtacggg gttatctggt gaaacccgca
     6721 aaaatgagcg gcaaaacgcc agccgtgggg gtggtgcatg agaatcgtgg actgaatccg
     6781 tatatcgaag atgtggcacg gcgagtggcg aaggcggggt atatcgccct ggcacctgac
     6841 ggattaagtt ccgttggagg ttatccggga aatgatgata aaggtcgtga gctgcaacag
     6901 caggttgatc caaccaaact gatgaatgat ttctttgccg caattgagtt tatgcaacgc
     6961 tatccgcaag cgacaggtaa agtgggtatt accggatttt gctatggcgg tggcgtatcg
     7021 aacgcggcgg ctgtcgcgta tccggaactg gcctgcgcgg tgccgtttta tggtcgtcag
     7081 gcacccactg ccgatgtggc gaagattgaa gcgcctttac tactccactt cgcggaactg
     7141 gacacccgaa tcaacgaggg ctggcctcct tacgaggcgg cgttgaaaga caataataag
     7201 gtttatgagg cttatatcta tccgggggtt aatcacggat tccataatga ttccacgccc
     7261 cgttatgaca aatctgccgc cgatcttgcc tggcaaagga cactgaaatg gttcgataaa
     7321 tatctctcct gataggttta tctcttacgg gattacgtct taaacaagta tgaaaaaata
     7381 gcgtgcgcaa aagtcgttct ttgcctaaaa tatcgctata tatggcgata tataacgaat
     7441 ggggtgaacg atgaataacc attttggtaa aggcttaatg gcgggattaa aagcaacgca
     7501 tgccgacagt gcggttaatg tgacaaaatt ctgtgccgat tataaacgcg gttttgtatt
     7561 aggctactca caccggatgt acgaaaagac cggagatcgc cagcttagcg cctgggaagc
     7621 gggtattctg acgcgccgct atggactgga taaagagatg gtaatggatt tctttcgtga
     7681 gaataattcc tgttctacgt tgcgcttttt tatggccggt tatcgcctcg aaaattgatc
     7741 aaacatacgt attatcttgc tttaattaat tacactaatg cttcttccct tcgttttagc
     7801 gccccgccgc agtatcatga tatcgataac cataataaat gtgtggtaaa tggcgcatcg
     7861 atcgcattat tgattttgcg attgaggcaa aatatatgcc aggtcctcgc aacggaataa
     7921 ctataaatga ctggagataa caccctcatc cattctcacg gcattaaccg tcgtgatttc
     7981 atgaagcttt gtgcagcatt agccgccacc atggggttaa gtagcaaagc cgctgcagag
     8041 atggccgaat cggttactaa cccgcagcgt ccgccagtta tctggattgg cgcgcaggag
     8101 tgcaccggtt gtacggaatc tctgcttcgt gcaacgcatc caacggtaga aaacctcgtg
     8161 ctggagacta tctctctgga atatcacgaa gtgctttccg ccgccttcgg tcatcaggtc
     8221 gaagagaaca aacataacgc tctcgagaag tacaaagggc agtatgtgtt agtggtggat
     8281 ggttccatcc cattaaaaga taacggtatt tattgcatgg ttgccggtga gccgattgtg
     8341 gatcacatcc gcaaagcggc ggaaggcgca gcagccatta tcgctattgg ttcctgctcc
     8401 gcgtggggcg gtgttgccgc agctggagtt aacccaactg gcgcagtcag cttgcaagaa
     8461 gttctgccag gcaaaaccgt tatcaatatt ccgggctgcc cgccgaaccc gcacaacttc
     8521 ctcgcgaccg ttgcgcacat catcacttac ggcaaaccgc cgaaactgga tgacaaaaac
     8581 cgtccgacct tcgcctatgg ccgtctgatt cacgaacact gcgaacgtcg cccgcacttc
     8641 gatgctggtc gttttgccaa agagttcggt gatgaaggcc accgcgaagg ctggtgcctg
     8701 taccacctcg gctgtaaagg gccagaaact tacggcaact gctcaacgct gcaattctgc
     8761 gatgttggcg gtgtgtggcc ggtggcgatt ggtcacccgt gctatggctg taacgaaaaa
     8821 ggtatcggct tccataaagg catccatcag cttgccaacg tcgaaaatca gactccgcgt
     8881 tcacagaaac cggatgttaa cgctaaagag ggcggcaacg tctctgcagg cgctattggt
     8941 ttgctcggcg gtgtggttgg gctggttgcc ggtgtcagcg tgatggcggt gcgtgaactg
     9001 ggtcgtcagc aaaagaaaga taacgctgac tcacggggag aataaccgtg aacagacgta
     9061 attttattaa agcagcctcc tgcggggcat tgctgacggg cgcgctgccg tctgtcagtc
     9121 atgcggctgc tgaaaaccgc ccgccaattc cgggatcgct ggggatgttg tacgactcga
     9181 ccttgtgcgt aggctgccag gcttgcgtca ccaagtgtca ggatatcaac ttccctgaac
     9241 gtaacccgca aggggaacag acctggtcga acaacgacaa actgtcgccg tataccaata
     9301 acatcattca ggtgtggacc agcggcacag gggtcaacaa agaccaggag gagaacggct
     9361 acgcgtacat taagaaacag tgtatgcact gcgtcgatcc gaactgtgtc tctgtgtgcc
     9421 cggtctctgc actgaaaaaa gatccgaaaa ccggcattgt ccattacgac aaagacgtgt
     9481 gcaccggttg ccgttactgc atggtcgcct gtccgtacaa cgtgccgaag tacgactaca
     9541 acaacccgtt tggtgcgctg cataagtgcg agctgtgcaa ccagaaaggt gtggaacgtc
     9601 tcgataaagg cggtctgcct ggctgcgtag aagtgtgccc ggcgggcgcg gtgattttcg
     9661 gtacgcgtga agagctgatg gcggaggcga aaaaacgtct ggcgctgaag cctggcagcg
     9721 aataccacta tccgcgtcag acgctgaaat ctggcgacac ttacctgcat acggtgccga
     9781 aatattatcc gcatctgtac ggcgagaaag agggcggcgg tactcaggtt ctggtactga
     9841 cgggtgtgcc ttatgaaaat ctcgacctgc cgaaactgga cgatctttct accggtgcgc
     9901 gttccgaaaa tattcaacac accctgtata aaggcatgat gctaccactg gctgtgctgg
     9961 cgggcttaac cgtgctggtt cgtcgcaaca ccaaaaacga ccatcacgac ggaggagacg
    10021 atcatgagtc atgatccaca accgctgggt ggcaaaatca tcagtaaacc ggtcatgatt
    10081 tttggaccgt taatcgtcat ctgtatgctc ctgattgtga agcgtctggt gttcggtctg
    10141 ggctctgtct ctgacctgaa cggcggcttc ccgtggggcg tgtggatcgc gtttgacctg
    10201 ctgattggca ccggctttgc ctgtggcggc tgggcgctgg cgtgggcggt atacgtcttt
    10261 aaccgtgggc aataccatcc gctggtgcgt ccggcgctgt tggcgagtct gtttggttac
    10321 tcactgggtg gcttgtcgat cactatcgac gtgggtcgct actggaacct gccgtacttc
    10381 tacattccgg gtcacttcaa cgtgaactcg gtactgttcg agacggcggt ctgtatgacc
    10441 atctacatcg gcgtgatggc tctggagttt gctccggcac tgtttgaacg tctgggctgg
    10501 aaggtgtcgc tacaacgtct gaacaaggtg atgttcttca tcatcgcgct cggtgcgctg
    10561 ctgccgacca tgcaccagtc ttcaatgggg tcgctgatga tctcggcggg ctacaaggtg
    10621 catccgctct ggcagagcta tgaaatgttg ccgctgttct cgctgctgac ggcgttcatc
    10681 atgggcttct cgattgtcat ctttgaaggt tcactggtgc aggcgggtct gcgtggcaac
    10741 ggtccggatg aaaagagcct gttcgtcaag ctgaccaaca ccatcagtgt gttgctggcg
    10801 attttcatcg ggctgcgctt tggcgagctt atctatcgcg acaagctgtc gttagcgttt
    10861 gccggtgact tctactccgt gatgttctgg attgaagtcc tgctgatgct cttcccgctg
    10921 gtcgttctgc gtgtggcgaa gctgcgtaat gattcccgca tgctgttcct gtcagcactg
    10981 agcgcactgt taggttgtgc aacctggcgt ctgacctatt cgttggtggc attcaacccg
    11041 ggcggcggtt acgcctactt cccgacctgg gaagaactgt tgatttctat tggttttgtg
    11101 gctattgaga tttgcgctta catcgtactc attcgtctac tgccgatact tcctccttta
    11161 aaacaaaacg atcataatcg tcatgaggcg agcaaagcat gagccagaga attactattg
    11221 atccggtaac ccgtattgag gggcatttac gcatcgattg cgaaatcgaa aatggcgtcg
    11281 tttcgaaagc atgggcttcc ggtaccatgt ggcgcggcat ggaagagatc gtgaaaaacc
    11341 gcgatccgcg cgatgcatgg atgattgtgc aacgtatctg tggcgtatgt actaccactc
    11401 acgcgctgtc ttccgttcgt gcggcagaaa gtgcgctgaa tatcgacgtt ccggttaacg
    11461 cgcaatacat ccgtaacatc attctggctg cgcacaccac gcatgaccat atcgttcatt
    11521 tctatcagct ttcggcgctg gactgggtgg acatcacttc tgcactgcaa gctgacccaa
    11581 ccaaagcctc cgaaatgctg aaaggcgttt cgacctggca tctgaacagt ccggaagagt
    11641 tcactaaagt tcagaacaag atcaaagatc tggttgccag cggtcagttg ggtattttcg
    11701 ccaatggcta ctggggtcac ccggcgatga aactgccgcc ggaagtgaac ctgattgcgg
    11761 tagcgcacta cctgcaggcg ctggagtgcc agcgtgacgc taaccgcgtc gtggcgctgc
    11821 tgggcggtaa aacgccgcac attcagaacc tggcagtgag tggtgtcgcg aacccaatca
    11881 acctcgacgg gttgggcgtg ttgaaccttg agcgcctgat gtacatcaag tctttcatcg
    11941 acaaactgag cgactttgtt gagcaggttt ataaggttga taccgcggtt atcgccgcgt
    12001 tctacccgga atggctgacg cgcggtaaag gtgcggtgaa ctacctgagc gtgccggaat
    12061 tcccgaccga cagcaaaaac ggcagcttcc tgttcccggg cggctacatt gagaatgcgg
    12121 atctgtcctc gtatcgtccg atcacttctc attccgatga atacctgatc aaaggcattc
    12181 aggaaagcgc gaagcactcc tggtataaag acgaagcgcc acaggcgccg tgggaaggta
    12241 ccaccattcc ggcttatgat ggttggtctg acgacgggaa atattcctgg gtgaaatcac
    12301 cgactttcta cggcaaaacg gtagaagtgg gtccgctggc taatatgctg gtgaaactgg
    12361 cggcaggtcg cgaatctacc cagaacaaac tgaatgaaat cgttgcgatt tatcagaaac
    12421 tgactggcaa cacgctggaa gtggcacagc tgcactccac gctgggccgt attattggtc
    12481 gtaccgttca ctgctgtgaa ttgcaggata tcctgcaaaa ccaatacagt gcactgatca
    12541 ccaatatcgg caaaggcgat cacaccacct ttgtgaagcc gaacattccg gcaacgggtg
    12601 agttcaaagg tgttggcttc ctcgaagcgc cgcgcggtat gctctctcac tggatggtta
    12661 ttaaagacgg tatcatcagc aactaccagg cggttgttcc atcaacctgg aactctggtc
    12721 cgcgtaactt caatgatgac gtcggtcctt acgagcagtc gctggtgggt acaccggttg
    12781 ccgatccgaa taaaccgctg gaagtggtgc gtaccattca ctccttcgac ccgtgcatgg
    12841 cctgtgcggt acacgtagtg gatgccgacg gcaacgaagt ggtttcagtg aaggttctgt
    12901 aatgcgtatt ttagtcttag gggtcggcaa tattttgctg accgatgaag ccatcggcgt
    12961 gcggattgtc gaagcgttag agcaacgata cattctgccg gattatgttg agatcctcga
    13021 tggcggcacg gcgggaatgg agctgcttag cgacatggca aatcgcgatc atctgattat
    13081 tgcggatgcc attgtgtcga aaaagaacac gccgggaacg atgatgatcc tgcgggatga
    13141 agaagttccg gcgttgttta ccaacaaaat ctctccgcat cagcttggcc tggccgacgt
    13201 attgtcggcc ctgcgcttca ccggtgagtt tccgaaaaag ctgaccctgg tcggcgtgat
    13261 cccggaatcg ctggagccac acatcggctt aacgccgacg gttgaagcaa tgattgaacc
    13321 tgcgcttgag ctggttctgg ctgcgctgcg cgaatcaggc gtggaagcca tcccacggga
    13381 ggcgattcat gactgaagag atagcaggtt tccagacctc cccgaaggcg caagtacagg
    13441 cagcgtttga agaaattgcc cggcgttcga tgcacgatct ctcttttctg catccttcaa
    13501 tgccggtgta tgtttctgat tttacgctgt tcgaaggtca gtggacgggg tgtgtgatca
    13561 ccccgtggat gctgagtgca gttatcttcc ccggcccgga tcaactctgg ccgctgcgca
    13621 aagtgagtga aaaaattggt ctgcaactgc cgtatggcac catgaccttt accgttggcg
    13681 aactggacgg tgtttcgcaa tacctctcct gttcgctgat gtcgccgctt tcgcacagca
    13741 tgtcgattga agaaggccaa cgcctgacgg atgactgcgc acgaatgatc ctttcgttgc
    13801 cagtcaccaa tccggatgta ccacacgcag ggcgtcgcgc cctgctgttt ggccgcagga
    13861 ggggcgaaaa tgcatgagtt gtcgctttgc cagagcgccg ttgaaattat ccaacggcag
    13921 gcggagcagc acgatgttaa gcgcgtcacc gccgtgtggc tggaaattgg cgcgctctcc
    13981 tgcgttgagg agagcgccgt ccgttttagt tttgaaattg tctgccacgg aacggtggcg
    14041 caagggtgcg atttacatat cgtctataaa cccgcccagg cctggtgctg ggattgcagc
    14101 caggtggtgg agattcatca gcacgatgcg cagtgtccgc tctgtcacgg cgagcggttg
    14161 cgtgtcgata ccggcgattc gctgatcgtc aaaagtattg aagttgaata agactgagga
    14221 caacgcctaa aaagcgcacg gacgggcccc tcgcccctcc ggggagaggg ttagggtgag
    14281 agggaacacc cgctaaaggt tctttttcgt gtttgtccgg caatcgaaat aaccggagtt
    14341 aacaatgtgt attggcgttc caggccaggt gctggctgtc ggtgaagata ttcaccagct
    14401 tgcgcaggtt gaagtatgtg gtatcaagcg cgacgtgaat atcgccctga tttgcgaagg
    14461 taaccctgcc gatctgctgg gccagtgggt gctggtacac gtcggatttg ccatgagcat
    14521 catcgacgaa gatgaagcca aagccacatt agacgcactg cgccaaatgg attacgacat
    14581 taccagcgcg tgatgattag cttttgtagg cctgataaga cgcggcagcg tcgcatcagg
    14641 cattgtgcac gattgccgga tgcggcgtga acgccttatc cggcctggat ggcttgctgc
    14701 gacgaacacc aacccttacc cctgacgctt atcttctgta tttgtctcaa aatcactggc
    14761 gtcatggcgc tcatgcaact gctcgttcag cggcccgttg gtgcggttaa caatacgccc
    14821 acgtttcact gccggacgtt cgcccacttc tttcgcccag cgttgcacat gtttataact
    14881 tcccgcatcc agaaactctg cggcatcata cacgccgccg agcaccacgt tgccaaacca
    14941 cggccaaatc gccatatccg caatggtgta ctcatcgccc gcaacaaact tatgctgcgc
    15001 cagttgctta tccagcacgt cgagcaggcg cttggcttcc atggtaaagc ggttgatggc
    15061 gtactcaatt ttcaccggcg cgtaatggta aaagtgacca aaaccaccgc cgaggaacgg
    15121 cgcggcaccc tgtaaccaga acagccagtt caacgtttca gtccgttttg ccagatcctg
    15181 cggcaggaag tagccaaatt tctccgccag ataaagcagg atcgaaccag attcaaacac
    15241 gcggatcggc ggattatgcg tatgatcgcg cagcgccggg atcttcgagt ttgggttcac
    15301 ttcgacaaag ccgctggaga attgatcgcc atcgccaata cgaatcagcc aggcgtcgta
    15361 ctctgcacca gtaacgccca gcgccagtag ctcctcaagc ataatcgtta ctttctgacc
    15421 gttcggcgtt cccagcgaat aaagttgcaa tggatgtttg ccaacgggca gcgttttttc
    15481 atgcgtcgga ccagaaaacg ggcgattgat attggcgaac gcgccgccag cggatttatc
    15541 ccacgtccag actttcgcgg gctgataagt attgtctgtc atagtgagtt gccttctgag
    15601 tggtagtgtt gaagcagtgt agcaggtcac ttgctgtaca gaatgtgaat tttttaaccc
    15661 tttgcgccag ccgctgcgat gaggtgtatg attagtctga cctctgtccg gttaatgtcg
    15721 ctgagcaggt aatcagagca caacaattac gccaactgct taggttgtat tgttaaaggt
    15781 aaagtgatga gcaaaggaac gaccagccag gatgccccgt tcgggacatt attgggctac
    15841 gccccaggcg gggtagcaat ctactcttca gattacagtt ctctcgatcc gcaggaatac
    15901 gaagatgacg ccgtattccg tagctatatc gacgacgaat atatgggcca caagtggcaa
    15961 tgcgttgaat ttgctcgccg ttttctcttt ctgaattacg gtgtggtctt tactgacgtg
    16021 ggtatggcgt gggagatttt ctcgctgcgc ttcctgcgag aagtggttaa tgacaacatc
    16081 ctgccattgc aggcatttcc taacggctcg ccgcgtgcgc cggtcgcggg tgcgcttctt
    16141 atctgggata aaggcggtga atttaaagac actggccatg tcgccatcat tacccaattg
    16201 catggcaaca aagtccgtat tgcggaacag aacgtgattc attccccgtt gccgcaaggg
    16261 caacagtgga cgcgcgagct ggagatggtg gtcgaaaacg gctgctatac ccttaaagac
    16321 acttttgatg acaccaccat tctgggctgg atgatccaga cggaagatac tgaatacagc
    16381 ttaccgcagc cggaaattgc aggcgagctg ctgaaaatca gtggagcgcg tctggaaaac
    16441 aaaggccagt ttgacggtaa atggctggat gaaaaagatc cgctacaaaa cgcctatgtg
    16501 caggccaacg gtcaggtgat caatcaggat ccgtatcatt actacaccat taccgagagt
    16561 gccgagcagg agctaattaa agccaccaac gagctgcatc tgatgtatct tcacgcaacc
    16621 gacaaggtgc tgaaagatga caacctgctg gcgctgttcg acattccgaa aatcctctgg
    16681 ccacgtttgc gtctctcctg gcagcgtcgc cgtcaccata tgatcactgg tcgtatggat
    16741 ttctgcatgg atgagcgtgg cctgaaggtt tacgaataca acgccgactc cgcctcctgt
    16801 cataccgaag cgggcttgat cctcgaacgt tgggcggagc agggctataa aggcaacggc
    16861 ttcaatccgg cggaagggct gattaacgaa ctggctggtg cctggaaaca cagtcgtgca
    16921 cgtccgtttg tccatatcat gcaggacaaa gatatcgagg aaaactatca cgcgcagttt
    16981 atggagcagg cgctgcacca ggcgggcttt gaaacgcgta tcttgcgcgg gctggatgaa
    17041 ctgggctggg atgctgccgg gcaactgatt gatggggaag ggcgactggt taactgcgtg
    17101 tggaaaacct gggcgtggga aaccgcgttt gatcagattc gtgaagttag cgaccgtgag
    17161 tttgctgcgg tgccaatccg taccggtcat ccgcaaaacg aagtgcgtct tatcgacgta
    17221 ttgctgcgcc cggaagtgct ggtctttgag ccgctgtgga cggtgatccc cggcaacaaa
    17281 gcgattctgc cgatcctctg gtcgctgttc ccgcaccatc gttacctgct ggataccgat
    17341 ttcactgtta atgatgaatt ggtgaaaaca ggttacgcag tgaaaccgat cgccggtcgc
    17401 tgtggtagca atatcgacct ggtcggccat catgaagagg tgctggacaa aaccagcggt
    17461 aaatttgccg agcagaaaaa catctatcag caactgtggt gtttgccgaa agtggacggt
    17521 aaatacattc aggtatgtac cttcaccgtt ggcggcaact acggtgggac gtgtttgcgc
    17581 ggtgatgaat cactggtcat caaaaaagag agtgatattg aaccgttaat tgtggtgaaa
    17641 aagtaataca tgttgtagca accccgtact gattatgaag aataattagt acggggatta
    17701 atcaaaatat tcaatattta tatattcaaa tatttattca tttcatatgt gaatatatta
    17761 accagtggaa tacctgtgtt ttgattattt ctaaaggttt tgaatgaatg cttattgtct
    17821 aatacacaag aaataacact ctttttatcg ttaaaaaatg atatttcact ttgcccatgc
    17881 cgttaaagtc actgataatg cgtccgttcg taaattcaaa atggcgtaat ctaatatatg
    17941 ctaaatttat ttgttggcct tgatatatat acggggcttt tgttattact tgctctggca
    18001 tttgtgttgt tctacgaagc aatcaatggt tttcatgaca cggcgaatgc ggtggcaacc
    18061 gttatttata ctcgtgccat gcagccacaa cttgctgtgg tgatggcggc attttttaac
    18121 ttttttggcg tgttattggg cggacttagc gttgcctatg ccattgtcca tatgttacca
    18181 accgatttgt tgctgaatat ggggtcaact cacggtctgg cgatggtctt ttccatgctg
    18241 ctggcggcga ttatctggaa cctgggaacg tggttctttg gtttaccggc ctccagttcg
    18301 cacaccttga ttggtgcgat tatcggcatc ggtttaacca acgcgctgtt aaccggctca
    18361 tcggtgatgg atgcgttaaa cctgcgtgaa gtaaccaaaa ttttctcctc gctgattgtt
    18421 tcccctatcg tcggcctggt cattgcgggt ggtctgattt tcctgctgcg ccgtttctgg
    18481 agtgggacga aaaagcgtga ccgtattcac cgcattccgg aagatcgcaa aaagaaaaag
    18541 ggcaaacgta aaccgccatt ctggacgcgt attgcgctga ttgtttccgc tgcaggcgtg
    18601 gcgttttcgc acggcgcgaa cgacgggcag aaagggatcg gcctggtaat gctggtactg
    18661 gtggggattg cccctgctgg cttcgtcgtc aatatgaatg cgtccggcta tgaaattacc
    18721 cgtacccgcg atgccgttac caacttcgaa cactacctgc aacagcatcc tgaactgccg
    18781 cagaagttga ttgccatgga acctccattg cctgcagcat cgactgatgg cacgcaagta
    18841 acagagtttc actgtcatcc ggcaaatacc tttgatgcga ttgcgcgcgt taaaacgatg
    18901 ctgccaggca atatggaaag ttacgagccg ttaagcgtga gtcagcgcag ccagctgcgc
    18961 cgcattatgc tgtgcatctc tgatacctcc gcgaagctgg cgaaactgcc aggcgtcagt
    19021 aaagaagacc agaacctgct gaagaaactg cgcagcgata tgttaagcac cattgagtac
    19081 gctccggtgt ggatcatcat ggcggtagca ctggcgctcg gcattggcac catgattggc
    19141 tggcgtcgtg tagcgatgac catcggtgag aagattggta agcgcggcat gacgtatgcg
    19201 caaggcatgg cggcacaaat gacggcggca gtgtctatcg gccttgccag ttatattggg
    19261 atgcctgtct ccacaacaca cgtcctctcg tctgcggtag cagggacgat ggtggtggac
    19321 ggcggtgggt tacagcgtaa aacggtaacc agcatcctga tggcgtgggt gtttacttta
    19381 ccggcggcaa tttttctttc tggtgggctg tactggatag cattgcagtt gatttaacct
    19441 tcctgaaaat gcccggtcct ggcgatcggg catttccatt tttgactagt gataaccacg
    19501 cgcggtcata aaatccgtaa tcgctttttc tgcatcaacc aacacctgtt ccagaggctg
    19561 attggcatcg atatcaacca gttgtgcacc accaaaggtt aactgtggcg ttatggcaat
    19621 cttcctcgcc agcgattccc gtttatggtc gggtttacgt gcacaggcaa cttcaaggtc
    19681 aacattgagt ttgatgacca gatcaggctt atggctcgcc atccagtgaa acgctttacg
    19741 ttcctggctt gccagccatg agacaaaacg accaccttca acgttaggtg ggaacaccgt
    19801 accatcgtaa gcgccaggaa tttggtcctg aggataacgg tcggttagaa caattaaccc
    19861 gcgacgacga caggcaagca tatgacgaaa gcgcagtaag cgacgggcga caaacgctgt
    19921 aattaccagc gccggaactg gtccaggcaa tttttttgct gttttcacct gatttcgttc
    19981 aattgtttta tgtaaggatt ttcccatcaa aggtaatttt gtcactgcac gaccgacatt
    20041 tccggcctgt tttcctaaat gaactctttc ggcagcacca tatttttcga caacggtaat
    20101 aagatgttca cacaccgttg acttgcctga accatcgcta ccaataacgg caattaatgg
    20161 aggtgttatt gactgcatga atatatcctt taatttaaat atccattaaa aatatttatt
    20221 tggttaatat gtttttatga aagcgtaatt caggtcaatg tcacaattaa ccattgtcac
    20281 aataaggttg aacggatatt ctgagcacgg accacgtaat attcaataca ttatttactg
    20341 ccgggttttt cgtgaatcaa cgtaatatgt caataattaa ttccacgcca gtgcgtgtta
    20401 ttgccattgt aggatgtgat ggttcaggta aatcgaccct cacggcaagc ctggtaaatg
    20461 aactggcagc aagaatgcca acagaacaca tttatctcgg gcaatcgtcc gggcgaattg
    20521 gcgaatggat ttcacagctc cctgttattg gcgcaccttg tgggtgttat ctgcgaagta
    20581 aagcggcaca tgtacacgaa aagccctcaa caccgcctgg caatattact gcgctggtta
    20641 tctatctgct ttcctgctgg cgggcgtaca agtttcgcaa aatgttgtgt aaaagccagc
    20701 aaggctatct gctcatcacc gaccgctacc cgcaggttga agtgccgggt tttcgctttg
    20761 atggcccgca attggcaaaa accacgggcg gtaacggttg gataaaaatg ttaaggcagc
    20821 gcgagttgaa gctgtaccaa tggatggcgt cttatttacc cgtattgttg attcgtcttg
    20881 gcattgatga acaaaccgcg tttgcgcgta aacctgacca ccaactggca gcgttacagg
    20941 aaaaaatcgc cgttacgccg caactgacat tcaatggcgc caagatcctt gaactggatg
    21001 gtcggcaacc agccgatgaa attatgcaag cgtcactacg cgcaattcac gccgcgcttt
    21061 cctgatcacc aaaatggata ggggatgatt taacgaatgg atgcactaca aacacaaact
    21121 gttaatagta caaccgcgcc gcagcccaac tacattccgg ggctgattgc ggtggtcggg
    21181 tgtgatggca ccggtaaatc cacgctgacc accgacctgg tgaaatcgct gcaacaacac
    21241 tggcaaaccg agcgccgcta tctggggctg ctctccggcg aagacggaga caaaatcaaa
    21301 cgattgccgt tggttggcgt ctggctggaa cggcgactgg cggccaaatc ctcgaaaacc
    21361 caaagcatga aaaccaaatc tccggcgcta tgggcggcgg tgattatgta ctgcttctcg
    21421 ctgcgaagaa tggcgaatct acgcaaggtt cagcgactgg cgcaaagtgg cgttctggtg
    21481 gtcagcgatc gcttcccgca ggctgaaatt tcgggctttt attatgacgg accggggatc
    21541 ggcgtcgaac gtgcgaccgg gaaaatcagc atgtttctgg cgcagcgcga acggagttta
    21601 taccaaaaaa tggcgcaata ccgtcctgaa ttaatcattc gtttggggat tgatattgat
    21661 actgccatct cccgcaagcc tgaccatgag tatgccgagc tgcaggacaa aatcggcgtc
    21721 atgtcgacga ttggctataa cggcacaaaa attcttgaga ttgattccag agcgccctac
    21781 agcgaagtgc tggaacaggt acagaaggcg gtttctctgg ttgccatcgt ttctgaccgc
    21841 cgaagtttaa cttaaggctg gcgggatatt ttttactatc aacgataagg tcaggctgaa
    21901 ttggctggtt ttaacatcaa acattggttt gcagatggcg cgtttcgcac cattattcgc
    21961 aatagcgcct ggttaggctc cagtaatgtc gagagcgcct tgctggggct gttggcgctc
    22021 tcgtgtgccg gtaaagggat gacgcccgcc atgtttggtg tactggtgat tgtgcaatcg
    22081 tacgccaagt cgatcagcga ttttattaag tttcagacat ggcagctggt ggttcagtac
    22141 ggaacgccag ccttgaccaa caataatccg cagcaatttc gcaatgtcgt ctcattttcc
    22201 ttctcgctgg atatcgtcag cggcgcggtg gcgattgtcg gcggcattgc cttactgcca
    22261 ttcctttccc attcattagg tctggatgac caaagctttt ggctggcggc gctgtattgc
    22321 acgctcattc cttcaatggc ttcctccacg ccgaccggca ttctgcgtgc ggtagatcgc
    22381 tttgatttga ttgctgtaca gcaggcgacg aaaccttttc tgcgcgcagc ggggagcgtc
    22441 gcagcctggt attttgactt tggttttgcg ggttttgtta ttgcctggta cgtgtcgaat
    22501 ctggttggcg gcaccatgta ctggtggttt gccgcgcgcg aattacgccg tagaaatatc
    22561 cataacgcct tcaaattgaa tctgtttgag tctgcccgac acattaaagg cgcgtggagt
    22621 tttgtctggt caaccaacat tgcccactcc atctggtcgg cgcgtaactc gtgcagcaca
    22681 gtgttggtgg ggatcgtgtt aggacccgct gccgctgggt tatttaaaat cgccatgaca
    22741 ttcttcgacg ccgccggaac gccagcgggt ttgctgggta aaagttttta cccagaggtg
    22801 atgcgtttag atccgcgcac caccagaccg tggttgttag gtgtgaagtc cggtttactg
    22861 gcgggcggaa tcggtattct ggtggcgctc gcggtgttga tcgtcggcaa gccgctcatt
    22921 tcgctggtgt ttggcgttaa gtatctcgaa gcgtatgacc tgattcaggt gatgctgggc
    22981 gcaattgtga tttcgatgct gggcttcccg caggagtcat tgctattgat ggcgggaaaa
    23041 cagcgcgctt ttctcgtggc gcaaaccatc gcctcaatcg gctacatcgt attgctgttc
    23101 atgttctgtc atctgtttgg tgtgctggga gcagcgtttg cttacttcgg tgggcaatgt
    23161 ctggacgtgg cgctctcgtt aattccaacc ttaaaggcat tttttcagcg ccattccttg
    23221 ctttataacg cagccggaga gaaatcctga tgatgagaaa atatttcccg cttgaggcga
    23281 gcgagcgtct atttgttgcg attgaagaag atgatgtcgt tgatgcacag gttagtttgc
    23341 cgccgactat tgcactaagt tgtaccaccg aaatcattca tgataattac gcgttatgcc
    23401 tgcaattctg gctcaatggc gttgatcgtc aggaattact gcgtttagtt cgcaaacaag
    23461 ccaaaggcga tgagctgact gctgatgagc gcaaacaatt taaatacatg cgagcgcgct
    23521 acaagcacct gcgttttgcg caacggctgt atttaaagaa gcatcaggcc ggatttttat
    23581 tcggcaaaac gaccgttttt ctggggcgtt ttcaggacgg ctttcgtaac ggtaagaaaa
    23641 atattgtgtc gtattacggc aacctgttac ggatttattt aagctctccg gtctggtcgt
    23701 tggtgaacta ttcattacgc catagccagt tagaaagcgt aagcagtttt attgcctatc
    23761 ggcaaaagca aatgcatacc ctgaaagaga ttattgccaa accgcgacta accggcaggg
    23821 aatttcatga tgtgcgaaaa atcatcagcc agcaggtttc ttattacgac accttaagat
    23881 cgcttgatcc tgaaaataaa gaggcgttac agatttcccg tttcctcgcc gccattaatg
    23941 gcctgatggg agataaacac gacgacatgg tggcggatga tatggaaaac cgccagtcgt
    24001 attatgcacc gttggcgctg gatagcgata tttgtcagcg cttagagctg ctgatttcgc
    24061 gttttccgct atagccggag caatgatgtc atctcgtttg attattgcgt tgattatcat
    24121 gctcttagcc ccgggtgtgc aggcacacaa tttcgtcact ggcaaaacgg ttacccctgt
    24181 ttatatccag gaaggcggcg agctgcttct gaatagcgac gacgaaattc actaccaaaa
    24241 gtggaacagc acacaattag cgggaaaagt ccgcattatt cagtatatcg ccgggcgtaa
    24301 gtcggcgaag aaaaagaact cactgttaat taaagctgtt gaagcggcaa actttccgca
    24361 ggatcgtttt cagcccacca ccattgtgaa caccgatgat gccatctttg gtactggcta
    24421 ttttgttgtt ggcaaaatcg aaaaaaataa acgccgctat ccgtgggcgc aattcgttat
    24481 tgacggcaat ggacagggac gcgttgcctg gcgattgccg gagcagagtt caaccattct
    24541 ggtgctgaat aaagccgggc aaattcagtg ggcaaaggac ggcagcctga cgccggaaga
    24601 ggtagatcat gtcattgcgt tggcgcagaa actgagcaat gaataaaaaa ctcaaaggat
    24661 tgagggcgtt agtgagatga agctggttga acactacatc atgcgtggta cgcgccgtct
    24721 ggtgctgatt atcgtcggtt ttctgatctt tattttcgcc agctactccg cacagcgtta
    24781 tctgaccgaa gcggcaaatg ggacattagc tcttgatgtt gtgctggata tcgttttcta
    24841 caaagtactg attgcactgg agatgttgtt acctgttggt ctgtatgtgt cagttggcgt
    24901 gacgctaggg cagatgtata ccgactcgga aattaccgct atctctgcgg cggggggcag
    24961 tccgggacgc ttgtacaaag ccgttcttta tctggcgata ccgctaagta tttttgtcac
    25021 ccttctgtcg atgtatggtc gtacgtgggc ttatgcgcag atttatcaac tggagcagca
    25081 gtcgcagtcg gagctggatg ttcgccagtt gcgggcaaag aaatttaaca ctaacgataa
    25141 cggacgaatg atcctttcgc agacggttga tcaggataat aatcgcctga ctgacgcgct
    25201 gatttatact tctactgcca atcgaacccg tattttccgc gcccgttcgg ttgatgtggt
    25261 tgacccatca cccgagaaac cgaccgttat gttgcacaac ggcactgcct atcttctcga
    25321 tcatcagggg cgtgacgaca acgaacagat ctaccgtaat ctgcaattac atctgaatcc
    25381 gctggatcaa agcccgaacg tcaaacgcaa agcgaaatcg gtcacggagc tggcgcgctc
    25441 cgcctttcct gccgatcatg ccgaactgca atggcgacaa agccgtggcc tgaccgcatt
    25501 gttgatggcg ctgctggcca tttcattaag tcgggtaaaa ccgtggcaag ggcgattttc
    25561 aacgttattg ccactgacgt tgctgtttat tgccattttt tatggcggcg acgtctgccg
    25621 tacactggtg gctaacggcg cgattcccct cattcctggt ttgtggttag tgcccggact
    25681 catgctaatg ggcctgctga tactggtcgc acgtgacttc tctttgctgc agaaattttc
    25741 ccgatgaatg ttttcagtcg ctatttaatc cgtcatctct ttctcggttt tgccgccgct
    25801 gcagggctat tgctgccgct ttttaccacc ttcaacctga ttaacgaact ggatggtgtc
    25861 agcccgggcg gttatcgctg gactcaggcg gtgctggtgg tgctaatgac cttaccgcgc
    25921 acactggtcg aactttcgcc gtttatcgcg ttattgggtg gtattgttgg actggggcag
    25981 ttatcgaaaa acagtgagct taccgccatt cgcagcacgg ggttttctat cttccgtatt
    26041 gcactggtgg cgctggttgc cgggatattg tggactgttt cgttaggcgc gatagatgag
    26101 tgggtggcgt cgccattgca gcaacaggcg ttgcaaatca aatcgaccgc caccgcgttg
    26161 ggggaggacg atgacattac cggcaatatg ctgtgggcca ggcgtggtaa tgaatttgtg
    26221 acagtgaaat cgctgaacga gcagggccag ccggtgggcg tggagatatt tcattatcgc
    26281 gacgatctct cgcttgaatc ctacatttat gcacgcagtg ccaccattga agacgacaaa
    26341 acgtgggtcc tgcatggtgt gaatcataaa aaatggctga atggcaaaga aacgctggaa
    26401 acattagata atcttgcctg gcaatcggcc ttcaccagta tgaatcttga agagttatca
    26461 atgccgggga atactttttc tgtccgtcag cttaatcatt acatccatta tttgcaggaa
    26521 accgggcaac ccagcagcga ataccgcctt gctctgtggg aaaaactggg gcaacccatc
    26581 ctgaccctgg cgatgatttt gctggctgtg ccgtttacct ttagtgcccc gcgttcgcct
    26641 gggatgggta gccgtctcgc tgtaggcgtc atcgtgggct tactcacctg gatcagctat
    26701 caaatcatgg ttaatctggg attgctattt gcgttaagcg cacctgttac cgcgctcggt
    26761 ttaccggtag cgtttgtgtt ggtggcgttg agcctggtgt attggtatga cagacaacat
    26821 taaacccgcc gtggcgcaaa caccacggcg ggctcacatt actgcttcag agtagcaaat
    26881 gcctgaatga tggcatcgat ttgctcacga ctgtgggcag catttacgct acagcgcagc
    26941 agcgtgatgc ctgcaggcgc ggcaggcggc aggataagat tcacatacac gcccggcgag
    27001 ataagctcgc gccagatgcg taaaccttcc tctttcgaac cgattattac tggcactacc
    27061 gggctaatat gtgaacttaa ctcataaccc agtttttgca gaccgtcata cagatggttg
    27121 gcgttatcca tcagcttttg ccgcagctct ggttgtgtgg caatagttct taacgacgag
    27181 cgcacggtcg caatgcagga cggagaagga gaggcggtaa aaatatacgg gcggctgccg
    27241 tagcgcagca cttccatcgc ttccgaaccg acagcaaaac cgccaatcga ggccagactt
    27301 ttactgaaag taccaacgat aatatccaca tcatcttcaa cgccgacagc ttcagccagt
    27361 ccgcgccccg ttgcgcctaa cacgacaaac gaatgcgctt catcgacaat caaataacca
    27421 ccgagacgac gtttgatatc aacaatttcc gccagcggcg caacgtcacc gagcatgctg
    27481 tagatgccct caacgatgat aatcgcctct ttcgcccgtt caccgaggcg aaccatgcgc
    27541 cgttccagat ctttagcatc gttatggcgg aaacgaataa tttccgcgcc accgagagaa
    27601 caggcgtcat aaatactggc gtggctgtcg gcatccagta acaccacggc gttatggtcg
    27661 gcaagtgcgc tgatgacgcc taaattagcg gtataaccgg ttgaaaaaac gatagcggta
    27721 gggcgattga aaaactcggc aatctctttc tctaacgcca ggtgcggcgc ataactgccg
    27781 tttgccatac gagaaccggt ggtgccggtg ccttgggccg ctaacgcggc ctgaccttcg
    27841 ctgatggcat catgattaaa ggttaaaccg agataattat tggtcccggc cagaatcact
    27901 tttttattgc cgatccggcc ttcggtggcg gaatacactt catcaataca ggtgccaaac
    27961 ggtgttaacc cgttgtcact aaattgcaga cgctcgccag caaggcgcgc atatttatcg
    28021 tatagcccca ttatttattc tccagccatg gaagtagagt ttccattaat tgcgcagggg
    28081 gtttgacatt cagcagaata ttaataggaa tggaaatatc gaatctgtct tccagcatca
    28141 ttaagagatc catcactttg atcgactcca ggccaagatc gttgacgaga tcgctgtcgg
    28201 gcttaatttc cacaccgttt tcgaccaaat cctgtaaaca ggaaaggatg taatccatta
    28261 ctatttcacg atttaccata gaaacgttaa cacaccttta aaacaagaca atcaaaatag
    28321 cccgttgcgt aatgcgtgtt ccaggctaat cccgggaaac caattgatat cttcagaaat
    28381 acggttatta cttgccgacc agtcggcgtg ggttaattca cgaattttcg agcgggtcag
    28441 cataggttct ttacctgcca ggcgactcaa cgcggtactg atatccgcaa ggcctgtgag
    28501 taccggcaga ggaatgtcaa ccattcgcac ggaaccacaa cgggctttgg cggcaagctg
    28561 tcgtacgcgt tgccagtcat agccaccggc gacgccatcg cataattcat aggtttgcgt
    28621 ctgtacagtt tcggcgctta accactgacc cactgcttgc gcaaaatcgg tgacgtgaag
    28681 aaaagagagt tgtgtatctg gcgcaccaag tcgtggcagc aggccgcgta gcatccagtc
    28741 aaacagcggt tttaactctt tatcgccggg accatacacg gctgtcgggc gaaaaacgcc
    28801 cagcgtaatt tcgtccgcca ttgctgccag tcgttgctcg gcgacgtatt tggattttgc
    28861 ataccaggag agctcgggat ggcgcgccgc cagcgaagag ataaacagaa aacgttggca
    28921 aaagccgctc tcttttgctg cttgcatcag gcgcaggctg ccgtcaacgt tacagcgggt
    28981 gaaaatctct tctttgtgcc cgcgcacttg cccggcgcaa tggaccacta cgctggctcc
    29041 ggcaaccagc tcgctaagtg aatgagtatc ttccagcgaa ccgcgcaccc aggtgagatt
    29101 atcattgacg tgagcgcggg cagtacgcgt caatgcgcga acgtgaaagc cgcgggcgag
    29161 caggttatcg ataatatatt taccgataaa cccggtagcg cccgtcaccg cgacagtttg
    29221 attcatgcca gggattcctg cacattaaga ctggcagcat aagccttctg ataacgtttt
    29281 ttcgcttccg cacgggcagg cttgccggag gacgttcggg gaatactgtg gggcggcaac
    29341 agatcgatag ccgcggtcac gccaaattcg ctttggatcc gtgccgccag cgcgtggata
    29401 agctgcccgc gacgttcttc gtcgctgatc cgacactgga tctgcaaaat gattttttcc
    29461 tgggcggtaa caaaagcaat cgcatcgcca gaatgaattt ccggttcctg ttccgctata
    29521 tattcaatat cctgcggcca gatattacgg ccacgaataa taatcagatc tttaatgcgt
    29581 ccggtgacat acagataacc gtccagcaga taaccgaggt cgccggtgtc taaccagccc
    29641 gtcgcggcaa tctcgtcttg cgaaacctgg tcgccaaagt aaccgctcat cagactggga
    29701 ccggagatgc aaatatggcc taccacacgt tccgcgaccg gcatacctgc ttcattgcgg
    29761 atttcaatac catgttccgg caacgctttg ccgcagttga cgaaagtcga tacggcgcgt
    29821 gtctctgcac ccggcgcgac ggctttaccc tgatattcga ggatgtcgcg atccacttcg
    29881 ttaaccacaa ccccggaggc ttcatcagag aagctgacag ccagcgcatt ttctgccagt
    29941 ccgtagcacg gcatgaaagt tttattgtca aagttaacct gacggaaaca ttcagcgaat
    30001 tgatggagtt gttctgcgga gatcggctca gcaccaatac cagcgacgcg ccagtaggaa
    30061 agatccagtt cagcgagatc tttttcattc acgcggcgtt ggcacaattc atagccaaac
    30121 ggcggcgcaa cggaaacggt gccgcgattt ttactgatca atttaagcca ttgcaaagga
    30181 cgcatggcaa aatcctgagt gcgcaaataa tctactgaaa gttgcgtggc gacggggggc
    30241 aggagaaagc cgaccagtcc catatcatgg tagaaaggct gccaggagac gcagcggtcg
    30301 ctagggcgta atttaatgcc atcgtggctt atagcacgta gattagccat cacttcgcga
    30361 tgggtgataa tgacgccacg gggaaaacgg gtgctgccgg aggtgtactg gaggtaggcg
    30421 atatcgttcg gaactggacg ctggagcgca acatcggctt ccggtaatgc cttaaaccaa
    30481 gcgtggctta aaacatgtaa ttcggggttg ttatgcgtcg cggcattgac cagtggcaac
    30541 tactcatcac cagtgataat ggctgcgggc tggcaacttg ccagtaaacc ctagagtttg
    30601 gcgctccagg aatcccgctg accgacgccc attggaatcg ccaacgggaa ggcgactaag
    30661 ccagcatact gacaggcaaa aaaagcctct acaaacccgc tactcgtttc ggcaatcagc
    30721 gccacgcgtt cgcctttttt caggttcagc gataacaacc gcttcgcacc agcttcggca
    30781 cgcgttttta atgtctgata ttccagttga tcttcaagtt ggcaacgtct gtcataaaaa
    30841 ttcattccgg ctctactcag agcggcgtag tccaaagcat caaccagcgt tggaaaatcg
    30901 gcatagcgca taggtaggga atgcgtaaag attttattag acatatacac cacgaaaaaa
    30961 ttggatatca aaaataaaat gacaataatg cggtggatta tataggcata aaagataaat
    31021 gagatagtat tttcatcggt gtgaaaatga acattgattt aaataatgag tttgagtttt
    31081 ttatttttat tatgaatgat atttaattta tgaatatatt aagaagaagc cttgtccggt
    31141 cgccttaaat cgacaaaagg cattgaggcg tttctgtgta gcctctgacc ttatcataat
    31201 cattaattaa tactatttat tcgtcgtttg tattcattga gttgattatt aagatgccgc
    31261 tcgttgctgt tgcaacctgt ttttcaccat taattatttg ttatccacag agatatggaa
    31321 tgcgatctgc tgatgattaa aattgaaaaa gtaattaata aaaatgacct taaggcattt
    31381 atcgcttttc cgtcatcact ttatcccgac gatccaaact ggataccccc tttattcatt
    31441 gaacgcaacg agcatttgtc tgcgaaaaat ccggggacgg accatatcat ctggcaggcg
    31501 tgggtggcaa aaaaagcggg gcagatagtt gggcgtatta ccgcgcaaat agataccttg
    31561 caccgtgagc gttacggtga agataccggt catttcggca tgattgacgc cattgatgat
    31621 ccgcaggatt ttgccgcgtt gtttggcgca gcggaagcgt ggttgaagtc acaaggtgca
    31681 agtaagatca gcgggccttt cagcctgaat atcaatcagg aaagcggatt actgattgaa
    31741 ggttttgaca caccaccctg tgcgatgatg ccacacggca aaccgtggta tgccgcgcat
    31801 attgaacaac tgggttatca caaaggcatt gatttactgg cgtggtggat gcagcgaacc
    31861 gatctcactt tctctccggc gctaaaaaaa ctgatggatc aggtgcgcaa aaaggtgacc
    31921 attcgctgca tcaatcgtca gcggtttgcc gaagagatgc agatcctgcg tgagattttc
    31981 aactctggct ggcagcacaa ctggggattt gtgccgttta ccgaacatga attcgcgacc
    32041 atgggcgacc aacttaaata tctggtgccg gacgatatga tctacatcgc tgagattgat
    32101 tctgcaccct gcgcgtttat tgtcggcttg cctaacatca acgaggcgat tgccgatctg
    32161 aacggatcgc tctttccctt cggctgggca aaattgctgt ggcgtttgaa agtcagcggt
    32221 gtgcgaactg cgcgagtacc gctgatgggc gtgcgcgacg agtatcagtt cagccgcatc
    32281 ggcccggtga ttgcgctgtt attgattgaa gccttacgcg atccgtttgc ccgccggaag
    32341 atcgatgcgc tggagatgta atggatcctc gaaaccaata caggcatgaa taacatgctg
    32401 gaacgcattg gggcggagcc gtacaagcgt taccgattgt atgaaaagca aatttaatac
    32461 cagatcctgt ttagcgcagg ggcggtcatt cgggagaggg tatagatgaa cagatttaac
    32521 accagatcct gttaagtgca ggggcggtcc cctcgccttg gggatagggt aaaactctgc
    32581 tcacattccc accttgttca tctccagcgg caccgccgag cgaatcaaat ggtggccttc
    32641 cagctcgata tcgtggagat tctttttcac ggtccgtaca tgatcgcgcg ctgcgcgctg
    32701 agcgacgtgc ggcagccgct gcaacaccgc gttgtagatc cgcgcatgct ggcgatcgat
    32761 ctgctttttt tgctgtggtc gatggtagag attatttacg ctggcaaaca ctgaattaaa
    32821 catcagatcg gtcaatgatt gcagcgtaaa caccagcacc tgattgtgag aagcctgaca
    32881 aatggcgaga tggaaagcgt gatccaactg cgcatgttcg atcagcgaaa tctctttgtg
    32941 gttctcactg gcggcgagca ttttttcata acagcgggtt atcacaacaa aatcagcctg
    33001 cgttcccagc gttgccgcca gccttgccga ttcgccctcc agtaatgcgc gaacgtcgag
    33061 cagatcgtac agcgttcgcg gctgcgtact gaacagatgg atcagcgggc tggtgtcctg
    33121 cacccgatta agccgtgcga catgagaatc gcgaccctgc gccgtttcaa taatcccgcg
    33181 cccgcgcagc acggtcagcc cttcacgcag tgcggagcgt gagaagccga gcttttcaca
    33241 cagtcgacgt tccgagggaa gcggctgacc gaccttcagt acgccgtcga taattaaccg
    33301 ttcgatactc tctgcaacca cttcgcaaat agggcgacgt tcatctttca ttcccggacc
    33361 tcgtgcacag gtaggaccaa tttttctgtt cttaactcgc aaaacacgca catcacgcaa
    33421 gtgattatgt tacatcaatt taacattaag ttaaccaaga caaggtcaca gagctggaaa
    33481 aaaaatggtc tgaccggtag ctaaagagat agacgaaaac gaaaagcccg cttaataact
    33541 gttcacagaa gcagcgcgca aaaatcagct gccacacaac aaagcgaagc ctactcatga
    33601 gcatcttgta cgaagagcgt cttgatggcg ctttacccga tgtcgaccgc acatcggtac
    33661 tgatggcact gcgtgagcat gtccctggac ttgagatcct gcataccgat gaggagatca
    33721 ttccttacga gtgtgacggg ttgagcgcgt atcgcacgcg tccattactg gttgttctgc
    33781 ctaagcaaat ggaacaggtg acagcgattc tggctgtctg ccatcgcctg cgtgtaccgg
    33841 tggtgacccg tggtgcaggc accgggcttt ctggtggcgc gctgccgctg gaaaaaggtg
    33901 tgttgttggt gatggcgcgc tttaaagaga gcctcgacat taaccccgtt ggtcgccgcg
    33961 cgcgcgtgca gccgggcgta tgtaacctgg cgatctccca ggccgttgcg ccgcataatc
    34021 tctattacgc gccggatcct tcctcacaaa tcgcctgttc aattggcggc aatgtggcgg
    34081 aaaatgccgg tggcgtccac tgcctgaaat atggtctgac cgtacataac ctgctgaaaa
    34141 ttgaagtgca aacgctggac ggcgaggcgc tgacgctggg atcggacgcg ctggattcac
    34201 ctggttttga cctgctggcg ctgttcaccg gatcggaagg tatgctcggc gtgaccaccg
    34261 aagtgacggt aaaactgctg ccgaagccgc ccgtggcgcg ggttctgtta gccagctttg
    34321 actcggtaga aaaagccgga cttgcggttg gtgacatcat cgccaatggc attatccccg
    34381 gcgggctgga gatgatggat aacctgtcga tccgcgcggc ggaagatttt attcatgccg
    34441 gttatcccgt cgacgccgaa gcgattttgt tatgcgaact ggacggcgtg gagtctgacg
    34501 tacaggaaga ctgcgagcgg gttaactaca tcttgttgaa agcgggcgcg actgacgtcc
    34561 gtttggcaca ggacgaagca gagcgcgtac gtttctgggc cggtcgcaaa aatgcgttcc
    34621 cggcggtagg acgtatctcc ccggattact actgcatgga tggcaccatc ccgcgtcgcg
    34681 ccctgcctgg cgtactggaa ggcattgccc gtttatcgca gcaatatgat ttacgtgttg
    34741 ccaacgtctt tcatgccgga gatggcaaca tgcacccgtt aatccttttc gatgccaacg
    34801 aacttggtga atttgcccgc gcggaagagc tgggcgggaa gatcctcgaa ctctgcgttg
    34861 aagttggcgg cagcatcagt ggggaacatg gcatcgggcg agaaaaaatc aatcaaatgt
    34921 gcgcccagtt caacagcgat gaaatcacga ccttccatgc ggtcaaggcg gcgtttgacc
    34981 ccgatggttt gctgaaccct gggaaaaaca ttcccacgct acaccgctgt gctgaatttg
    35041 gtgccatgca tgtgcatcac ggtcatttac cttttcctga actggagcgc ttttgatgct
    35101 acgcgagtgt gattacagtc aggcgctgct ggagcaggtg aatcaggcaa ttagcgataa
    35161 aacgccgctg gtgattcagg gcagcaatag caaagccttt ttaggtcgcc cagtcaccgg
    35221 gcaaacgctg gatgtgcgtt gtcatcgcgg cattgttaat tacgacccga ccgagctggt
    35281 gataaccgct cgtgccggaa cgccgctggt ggcgattgaa gcggcgctgg agagcgccgg
    35341 gcaaatgctt ccctgtgagc cgccgcatta tggtgaagaa gccacctggg gcgggatggt
    35401 cgcctgcggg ctggcggggc cgcgtcgccc gtggagcggt tcggtccgcg attttgtcct
    35461 cggcacgcgc atcattaccg gcactggaaa acatctgcgt tttggtggcg aagtgatgaa
    35521 aaacgttgcc ggatacgatc tctcacggtt aatggccgga agctacggtt gtcttggcgt
    35581 gctcactgaa atctcaatga aagtgttacc gcgaccgcgt gcctctctga gcctgcgtcg
    35641 ggaaatcagc ctgcaagaag ccatgagtga aatcgccgag tggcaactcc agccattacc
    35701 cattagtggc ttatgttact tcgacaatgc gttgtggatc cgccttgagg gcggcgaagg
    35761 atcggtaaaa gcagcgcgtg aactgctggg tggcgaagag gttgcgggtc agttctggca
    35821 gcaattgcgt gaacaacaac tgccgttctt ctcgttacca ggtaccttat ggcgcatttc
    35881 attacccagc gatgcgccga tgatggattt acccggcgag caactgatcg actggggcgg
    35941 ggcgttacgc tggctgaaat cgacagccga tgacaatcaa atccatcgca tcgcccgcaa
    36001 cgctggcggt catgcgaccc gctttagtgc cggagatggt ggctttgccc cgctatcggc
    36061 tcctttattc tgctatcacc agcagcttaa acagcagctc gacccttgcg gcgtgtttaa
    36121 ccccggtcgc atgtacgcgg aactttgagg agcaggctat gcaaacccga ttaaccgaag
    36181 agatgcggca gaacgcgcgc gcgctggaag ccgacagcat cctgcgcgcc tgtgtccact
    36241 gcggattttg taccgcaacc tgcccaacct atcagcttct gggcgatgaa ctggacgggc
    36301 cgcgcgggcg catctatctg attaaacagg tgctggaagg caacgaagtc acgcttaaaa
    36361 cacaggagca tctcgatcgc tgcctcactt gccgcaattg tgaaaccacc tgtccttctg
    36421 gtgtgcgcta tcacaatttg ctggatatcg ggcgtgatat tgtcgagcag aaagtgaaac
    36481 gcccactgcc ggagcgaatg ctgcgcgaag gattgcgcca ggtagtgccg cgtccggcgg
    36541 tcttccgtgc gctgacgcag gtagggctgg tgctgcgacc gtttttaccg gaacaggtca
    36601 gagcaaaact gcctgctgaa acggtgaaag ctaaaccgcg tccgccgctg cgccataagc
    36661 gtcgggtttt aatgctggaa ggctgcgccc agcctacgct ttcgcccaac accaacgcgg
    36721 caactgcgcg agtgctggat cgtctgggga tcagcgtcat gtcagctaac gaagcaggct
    36781 gttgtggcgc ggtggactat catcttaatg cgcaggagaa agggttggca cgggcgcgca
    36841 ataatattga tgcctggtgg cccgcgattg aagcaggtgc cgaggcaatt ttgcaaaccg
    36901 ccagcggttg cggcgcgttc gtcaaagagt atgggcagat gctgaaaaac gatgcgttat
    36961 atgccgataa agcgcgtcag gtcagtgaac tggcggtcga tttagtcgaa cttctgcgcg
    37021 aggaaccgct ggaaaaactg gcaattcgcg gcgataaaaa actggccttc cactgcccgt
    37081 gtaccctaca acatgcgcaa aagctgaacg gcgaagtgga aaacgtgtta cttcgtcttg
    37141 gatttacctt aacggacgtt cccgacagcc atctgtgctg cggttcagcg ggaacatatg
    37201 cgttaacgca tcccgatctg gcacgccagc tgcgggataa caaaatgaat gcgctggaaa
    37261 gcggcaaacc ggaaatgatc gtcaccgcca acattggttg ccagacgcat ctggcgagcg
    37321 ccggtcgtac ctctgtgcgt cactggattg aaattgtaga acaagccctt gaaaaggaat
    37381 aaaaaaatga aaactaaagt cattcttagc cagcaaatgg cgagtgcaat tattgccgca
    37441 ggtcaggaag aggcgcagaa aaataactgg tctgtttcca ttgctgttgc cgatgacggc
    37501 ggtcatctgc tggcgttaag tcgcatggac gattgcgcgc cgattgcggc ttacatctcc
    37561 caggagaaag cgcgtaccgc cgcgctgggg cgtcgtgaaa ctaagggcta tgaagagatg
    37621 gtgaacaacg gacgtaccgc gttcgtgact gcgccgttac tgacgtcgct ggaaggcggc
    37681 gtaccggttg ttgttgatgg gcaaattatt ggtgccgtgg gcgtttctgg tttaaccgga
    37741 gcacaggatg cgcaggtcgc gaaagcggca gcagcggtgt tggcgaaata agcgaaaacg
    37801 aggagagaaa caatgagtca aaccataacc cagggccgtt tacgcattga cgccaatttt
    37861 aaacgttttg tggatggaga agttttgccg ggcgtggaac tggacgctgc tgcattctgg
    37921 cacaatgttg atgagatcgt tcacgatctg gcaccagaaa atcgtcagtt gctggcagaa
    37981 cgcgatcgca ttcaggcggc gcttgatgag tggcatcgca gcaatccggg gccggtaaaa
    38041 gataaagcgg cctataaatc tttccttcgt gaactgggct acctggtgcc gcaaccggag
    38101 cgcgtgacgg tggaaaccac gggcattgac agcgaaatca ccagtcaggc ggggccacag
    38161 ctggtggttc cggcaatgaa cgcccgctac gcgctgaacg cggcgaacgc tcgctggggc
    38221 tcactgtacg atgcgttata cggcagcgac atcatcccgc aggaaggggc gatggtcagt
    38281 ggctacgatc cgcagcgcgg tgagcaggtt atcgcctggg ttcgacgttt cctcgatgaa
    38341 tcactaccgc tggaaaacgg cagctatcag gatgtggtgg cgtttaaggt ggtcgataaa
    38401 caattacgca tccagttgaa aaatggtaaa gaaaccacgt tacgtactcc agcacagttt
    38461 gtcggttacc gtggcgatgc cgctgcgccg acctgcattt tgctgaaaaa taacggcctg
    38521 catattgaac tgcaaattga tgccaacggg cggattggca aagacgatcc ggcgcacatc
    38581 aacgatgtta tcgtcgaagc tgctatcagt accattctcg actgcgaaga ttcggtcgcg
    38641 gcggttgatg cggaagataa aatcctgctg taccgcaatc tgctgggcct gatgcagggg
    38701 actctgcaag agaaaatgga gaaaaacggt cggcaaatcg tacgtaaact gaatgacgat
    38761 cgtcagtaca ccgccgccga tggctctgaa atttctctgc acggacgctc gttgctgttt
    38821 atccgcaacg tgggtcattt gatgaccatt cctgtgattt gggacagcga aggcaatgaa
    38881 atcccggaag gtattcttga tggcgtcatg actggcgcga ttgccctcta tgatttaaaa
    38941 gtgcagaaaa actcgcgcac tggcagcgtc tatattgtga aaccgaaaat gcacggtccg
    39001 caggaagtgg cgttcgccaa caaactgttt acccgcattg agacaatgct cggtatagta
    39061 ccgaataccc tgaaaatggg cattatggat gaagaacgcc ggacctcgct gaacttgcgg
    39121 agctgtatcg ctcaggcgcg caaccgcgtg gcgttcatca ataccggttt cctcgaccgt
    39181 accggcgatg aaatgcattc ggtgatggaa gctggcccga tgctgcgtaa aaaccagatg
    39241 aaatcgacgc cgtggatcaa agcctacgaa cgtaataacg tgctttccgg tctgttctgt
    39301 gggctgcgcg gtaaagcgca aatcggtaaa ggcatgtggg caatgccgga cctgatggca
    39361 gacatgtaca gccagaaggg cgaccaactg cgtgccgggg caaacacagc ctgggttccg
    39421 tcaccaaccg ctgctacgct ccatgcgctg cactaccacc aaaccaacgt acagagcgtg
    39481 caagccaaca ttgcccagac cgagttcaat gctgaatttg aaccgctgct ggacgatctg
    39541 ctgactattc cggtggctga aaacgctaac tggtcggcgc aagagatcca acaagagctg
    39601 gataacaacg tgcaggggat tctggggtac gtggtgcgtt gggtggagca ggggattggt
    39661 tgttcaaaag tgccggatat tcacaacgtg gcgctgatgg aagaccgcgc aacgctgcgt
    39721 atctccagcc agcatatcgc caactggtta cgtcacggta ttctgaccaa agaacaggtg
    39781 caggcgtcgc tggagaatat ggcgaaagtg gttgatcagc aaaatgctgg cgatccggct
    39841 tatcgtccga tggcggggaa tttcgctaac tcgtgtgctt ttaaagctgc cagcgattta
    39901 atcttcctcg gcgtgaaaca gccaaacggt tataccgaac cgttattaca cgcctggcgt
    39961 ttacgcgaaa aagaaagtca ttaatagcgt caaaaaagcc ccggcaagtt ttattgtcgg
    40021 ggctatgata gtaactgcgg tagtacattt atcccggaaa taaccggagc gattatcagg
    40081 gtttaacaat attaaatacc cgtagcgcat tactagcggg cctaaaacaa caatatggac
    40141 atcttaacga tactctgcgc ctgcgtgtgc ggaacagaaa cccttacccg atatacttgt
    40201 tatgccagag gtagcaattg cgttagcggc gtactgttgc tgtaaagtat aacgcctata
    40261 ttttacagtt cagtcctctt ttgcttatta caaccttaag tatatcaagc atataaagat
    40321 aataagagac tgaacaatat ggttacctgg acccaaatgt atatgccgat gggaggactg
    40381 gggctatccg ctctggtcgc cctgatcccg ataatattct tcttcgttgc actcgcggta
    40441 ttacgtctga aaggacatgt cgctggagca ataaccctta tattatctat cctgattgcg
    40501 atattcgcct ttaaaatgcc gattgatatg gcatttgctg ctgcgggcta tggctttatt
    40561 tatggattat ggccaattgc gtggattatt gtcgcggcgg tgttcctgta taaattaacc
    40621 gttgccagcg ggcagttcga tattatccgc agctcggtta tctccatcac cgacgatcag
    40681 cgtttgcagg tgttactgat tggtttctcc tttggtgcgt tgctggaagg agcggctggc
    40741 tttggtgcgc cggtggcgat taccggtgcg ctgctggtgg gcctgggctt caaaccgtta
    40801 tacgcggcgg ggctgtgtct gattgccaat actgcgccgg tggcgtttgg tgcgttgggc
    40861 gtgccgattc tggtcgccgg tcaggtaacg ggaatcgatc cgttccacat tggcgcaatg
    40921 gcgggacgtc agttaccgtt cctgtcggtt cttgtgccgt tctggctggt agcaatgatg
    40981 gacggctgga aaggggtgaa agagacgtgg ccagcggcgc tggttgctgg gggaagcttc
    41041 gctgtcactc agttctttac ctctaactat attggtccgg aactgccgga tattacttcg
    41101 gcgctggtga gtatcgtctc actcgcttta ttccttaaag tctggcggcc gaaaaatacc
    41161 gaaacggcaa tcagcatggg gcaatccgca ggtgcgatgg tggtaaataa gccatcttct
    41221 ggcggtcccg tgccttcaga atatagtctg gggcaaatca ttcgtgcgtg gtcaccgttt
    41281 ttaatcttaa cggtgctggt caccatctgg accatgaagc cgtttaaagc gttatttgct
    41341 ccgggcggcg cgttttattc actggtgatt aatttccaga tccctcattt gcatcaacaa
    41401 gtgttgaaag cggcacccat tgtcgcccaa ccaacgccaa tggatgcggt gtttaaattc
    41461 gaacctctct cggctggcgg caccgctatt tttattgcgg cgattatctc tatcttcatc
    41521 ctcggcgtgg gcatcaagaa agggattggc gtctttgccg aaacgctaat tagtctgaag
    41581 tggccgatat tgtcgattgg catggtgctg gcattcgcct tcgtcaccaa ctattccggc
    41641 atgtccacca cgctggcgct ggtactggca ggtacaggcg tgatgttccc gtttttctca
    41701 ccgtttctcg gctggctggg cgtattcctt accgggtcgg acacttcttc aaacgccctg
    41761 tttggttcac tgcaatcgac cacggcgcag caaatcaacg tctctgacac tctgctggtg
    41821 gcagcaaata ccagcggcgg cgtaactggc aagatgatct ccccgcaatc tatcgccgtg
    41881 gcctgcgccg cgacgggcat ggtgggccga gaatctgaac tgttccgcta caccgtgaag
    41941 cacagtctga tttttgccgg cgttatcggc gttatcaccc tgctgcaggc gtatgtgttt
    42001 accgggatgt tagtctcgta aatcaataaa gccggatgaa ggtaaatatc acccggcttt
    42061 ccatttgtct gccgtttatc tcgccaggga accgttttta cgattggctt ctttatacgt
    42121 agtctccgtt tcacgatgcg aattgtttaa tatttataca acttggcttc tgtgctggtt
    42181 ataaagtacc cactttcatg agatcaattt tagtatgtta ttacattgct tcatctgttg
    42241 ctttcatgac gaatggcgtt ttttctctta tttatatttt tcactagaac aatgagtaga
    42301 tcaaaattcc caaaactccc aaaaacataa gattattcgc aaagctgatt ttgcagaata
    42361 atgagaactt ttaagatttg tcgaggatca attttaagca atttatctcc acttgttaca
    42421 taaaagatgc cgttaggtta ataagttaat cttgctataa cagtgttcgt tactgcctat
    42481 taataaagat atttgggata gggtaacgtg gatgaggatt cgaaatcatc cgtcttgcgg
    42541 aaagtgaaat gttggcagat gttaatatta tgtatgtatt ttaactccaa caacacacca
    42601 atcattttgt tagaaataag aaaatttatg gttaaataat ttggtgatta tgaagcatta
    42661 aatcgtaatg ccaaatattg ttcgctttgg agagtattat tgcctgtttg tgtctgttga
    42721 gcagaaaaat catctgaatt tgaatacttt tattaggcgc gaagaaatga aaaaatcatt
    42781 aaaatcatta atttatatga atatttcaga gttggagttc gaatgttgac acctgagtat
    42841 ttcctgctaa aaatagccct gctttacgga tggttgtatt gcaataacac gaaagtagta
    42901 agagattaaa ggtatctgtc agcgataact ctgcaagtga tgacattttg taaaccaaat
    42961 aatgtaaatc agcaatgtat cttttcactt aattataaca aatgtggtat tcactaagaa
    43021 tctgatacga gcttacatca cgactatttt agtctcatcg cgggatacag ggatgcaccg
    43081 atggatctca gcacaccgta atgcaatacc tgaacaaata gaacagtaaa gtggttcagt
    43141 attaatgaaa gctcttattt tccgttggga aaacttaaaa tagatgagaa ggctttttga
    43201 tttcttatgt gatctcattt cgagtattga gaaatttgtg atttttaaat ttttcaataa
    43261 gccctgtaag ggattggggt tttatgagaa ataaagagac gtattgtctt tgtttaaac
//
LOCUS       DAJVQH010000016        43213 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJVQH010000016 DAJVQH010000000
VERSION     DAJVQH010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.016"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..180
                     /locus_tag="OQM87_001027"
     CDS             <1..180
                     /locus_tag="OQM87_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1507255.1"
                     /translation="PYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            205..2804
                     /locus_tag="OQM87_001028"
                     /pseudo
     CDS             205..2804
                     /locus_tag="OQM87_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            2795..3865
                     /locus_tag="OQM87_001029"
     CDS             2795..3865
                     /locus_tag="OQM87_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1507256.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESSDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQVIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            3877..4419
                     /locus_tag="OQM87_001030"
     CDS             3877..4419
                     /locus_tag="OQM87_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1507257.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGAGVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIQLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4427..4942
                     /locus_tag="OQM87_001031"
     CDS             4427..4942
                     /locus_tag="OQM87_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1507258.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTLDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4908..5645
                     /locus_tag="OQM87_001032"
     CDS             4908..5645
                     /locus_tag="OQM87_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS1507259.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5756..6766
                     /gene="pyrD"
                     /locus_tag="OQM87_001033"
     CDS             5756..6766
                     /gene="pyrD"
                     /locus_tag="OQM87_001033"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCS1507260.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6940..7482
                     /gene="zapC"
                     /locus_tag="OQM87_001034"
     CDS             6940..7482
                     /gene="zapC"
                     /locus_tag="OQM87_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCS1507261.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7479..8588)
                     /gene="ycbX"
                     /locus_tag="OQM87_001035"
     CDS             complement(7479..8588)
                     /gene="ycbX"
                     /locus_tag="OQM87_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCS1507262.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8832..10940
                     /gene="rlmKL"
                     /locus_tag="OQM87_001036"
     CDS             8832..10940
                     /gene="rlmKL"
                     /locus_tag="OQM87_001036"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCS1507263.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10952..12859
                     /locus_tag="OQM87_001037"
                     /pseudo
     CDS             10952..12859
                     /locus_tag="OQM87_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            12989..14242
                     /gene="pqiA"
                     /locus_tag="OQM87_001038"
     CDS             12989..14242
                     /gene="pqiA"
                     /locus_tag="OQM87_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCS1507264.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAVVIFLASIMVPTLKMSAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14247..15887
                     /gene="pqiB"
                     /locus_tag="OQM87_001039"
     CDS             14247..15887
                     /gene="pqiB"
                     /locus_tag="OQM87_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCS1507265.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15884..16447
                     /gene="pqiC"
                     /locus_tag="OQM87_001040"
     CDS             15884..16447
                     /gene="pqiC"
                     /locus_tag="OQM87_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCS1507266.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16703..16870
                     /gene="rmf"
                     /locus_tag="OQM87_001041"
     CDS             16703..16870
                     /gene="rmf"
                     /locus_tag="OQM87_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCS1507267.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16940..17458)
                     /gene="fabA"
                     /locus_tag="OQM87_001042"
     CDS             complement(16940..17458)
                     /gene="fabA"
                     /locus_tag="OQM87_001042"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCS1507268.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKIT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17527..19287)
                     /locus_tag="OQM87_001043"
     CDS             complement(17527..19287)
                     /locus_tag="OQM87_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCS1507269.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19472..19924
                     /gene="matP"
                     /locus_tag="OQM87_001044"
     CDS             19472..19924
                     /gene="matP"
                     /locus_tag="OQM87_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCS1507270.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20000..21046)
                     /gene="ompA"
                     /locus_tag="OQM87_001045"
     CDS             complement(20000..21046)
                     /gene="ompA"
                     /locus_tag="OQM87_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCS1507271.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21402..21911)
                     /gene="sulA"
                     /locus_tag="OQM87_001046"
     CDS             complement(21402..21911)
                     /gene="sulA"
                     /locus_tag="OQM87_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCS1507272.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22130..22759
                     /gene="sxy"
                     /locus_tag="OQM87_001047"
     CDS             22130..22759
                     /gene="sxy"
                     /locus_tag="OQM87_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCS1507273.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22722..24884)
                     /gene="yccS"
                     /locus_tag="OQM87_001048"
     CDS             complement(22722..24884)
                     /gene="yccS"
                     /locus_tag="OQM87_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCS1507274.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(24894..25340)
                     /locus_tag="OQM87_001049"
     CDS             complement(24894..25340)
                     /locus_tag="OQM87_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCS1507275.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25463..27517
                     /gene="helD"
                     /locus_tag="OQM87_001050"
     CDS             25463..27517
                     /gene="helD"
                     /locus_tag="OQM87_001050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCS1507276.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSDVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27549..28007)
                     /gene="mgsA"
                     /locus_tag="OQM87_001051"
     CDS             complement(27549..28007)
                     /gene="mgsA"
                     /locus_tag="OQM87_001051"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCS1507277.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28103..28765)
                     /locus_tag="OQM87_001052"
     CDS             complement(28103..28765)
                     /locus_tag="OQM87_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCS1507278.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            28938..29351
                     /locus_tag="OQM87_001053"
     CDS             28938..29351
                     /locus_tag="OQM87_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCS1507279.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29396..29713)
                     /gene="hspQ"
                     /locus_tag="OQM87_001054"
     CDS             complement(29396..29713)
                     /gene="hspQ"
                     /locus_tag="OQM87_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCS1507280.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(29771..30961)
                     /gene="rlmI"
                     /locus_tag="OQM87_001055"
     CDS             complement(29771..30961)
                     /gene="rlmI"
                     /locus_tag="OQM87_001055"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCS1507281.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31056..31334
                     /gene="yccX"
                     /locus_tag="OQM87_001056"
     CDS             31056..31334
                     /gene="yccX"
                     /locus_tag="OQM87_001056"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCS1507282.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31331..31660)
                     /gene="tusE"
                     /locus_tag="OQM87_001057"
     CDS             complement(31331..31660)
                     /gene="tusE"
                     /locus_tag="OQM87_001057"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCS1507283.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31751..32410)
                     /gene="yccA"
                     /locus_tag="OQM87_001058"
     CDS             complement(31751..32410)
                     /gene="yccA"
                     /locus_tag="OQM87_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCS1507284.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32617..32704)
                     /locus_tag="OQM87_001059"
     tRNA            complement(32617..32704)
                     /locus_tag="OQM87_001059"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32668..32670),aa:Ser,seq:tga)
     gene            33130..34248
                     /gene="hyaA"
                     /locus_tag="OQM87_001060"
     CDS             33130..34248
                     /gene="hyaA"
                     /locus_tag="OQM87_001060"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCS1507285.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLTKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34245..36003
                     /gene="hyaB"
                     /locus_tag="OQM87_001061"
                     /pseudo
     CDS             34245..36003
                     /gene="hyaB"
                     /locus_tag="OQM87_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            36022..36729
                     /gene="hyaC"
                     /locus_tag="OQM87_001062"
     CDS             36022..36729
                     /gene="hyaC"
                     /locus_tag="OQM87_001062"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCS1507286.1"
                     /translation="MQQKSDNIVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36726..37313
                     /locus_tag="OQM87_001063"
     CDS             36726..37313
                     /locus_tag="OQM87_001063"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCS1507287.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37310..37708
                     /gene="hyaE"
                     /locus_tag="OQM87_001064"
     CDS             37310..37708
                     /gene="hyaE"
                     /locus_tag="OQM87_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCS1507288.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37705..38562
                     /locus_tag="OQM87_001065"
     CDS             37705..38562
                     /locus_tag="OQM87_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCS1507289.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38696..40240
                     /gene="appC"
                     /locus_tag="OQM87_001066"
     CDS             38696..40240
                     /gene="appC"
                     /locus_tag="OQM87_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCS1507290.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40252..41388
                     /gene="appB"
                     /locus_tag="OQM87_001067"
     CDS             40252..41388
                     /gene="appB"
                     /locus_tag="OQM87_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1507291.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41400..41492
                     /gene="cbdX"
                     /locus_tag="OQM87_001068"
     CDS             41400..41492
                     /gene="cbdX"
                     /locus_tag="OQM87_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCS1507292.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41572..42870
                     /gene="appA"
                     /locus_tag="OQM87_001069"
     CDS             41572..42870
                     /gene="appA"
                     /locus_tag="OQM87_001069"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCS1507293.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(42985..>43213)
                     /locus_tag="OQM87_001070"
     CDS             complement(42985..>43213)
                     /locus_tag="OQM87_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-tyrosine kinase"
                     /protein_id="HCS1507294.1"
                     /translation="LAVSDAAVVGRSVGTSLLVARFGLNTAKEVSLSMQRLEQAGVNI
                     KGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
BASE COUNT        10745 a        10524 c        11504 g        10440 t
ORIGIN      
        1 ccttatttca ttaccctgac agacctgaaa ataggtggaa aagtagttaa aaatcaaatg
       61 atttccccct ttgataaata ccaattctct ctgccaaagg gggccaaaaa tagcagcgta
      121 acgtatcgaa ccatcaatga ctacggggcg gaaacgccgc aactcaactg taaatcgtaa
      181 gccgtcttca gttaagagag cgagatgtat agaactcatc gacaacacag cctgttaagc
      241 tctggtggag tgccatcgtt tattggtggg ctggtggtgt ttgtgtcggc agcgttcaat
      301 gcacaagctg aaacctggtt cgatcctgct tttttcaaag atgatccctc aatggtggcc
      361 gatttgtctc gtttcgaaaa aggacaaaaa ataacgccag gggtttatcg tgtcgatatt
      421 gttctgaatc agacaattgt agatacgcgc aacgtcaatt ttgttgagat aacgccagag
      481 aaggggattg ccgcctgttt gacgactgaa agcctggatg caatgggtgt taatactgat
      541 gcgtttccgg cttttaaaca actggacaaa caagcgtgtg tgccattggc ggagattatt
      601 ccggatgcca gcgtaacgtt taatgtgaat aaactccgtc tggaaatttc agtaccgcaa
      661 atcgccatca aaagtaatgc ccgtggttat gtcccccctg aacgttggga tgaagggatc
      721 aacgcgctat tactgggata ttcgtttagc ggggctaaca gtattcatag cagcgcagac
      781 agtgattctg gcgacagcta ttttctgaat ttaaacagtg gcgttaattt aggcccatgg
      841 agattgcgca acaattcaac atggagtcgc agtagtggcc aaaccgcaga atggaagaat
      901 ctcagcagct atttgcagcg ggcggttatt ccactaaaag gcgaactgac cgtaggtgat
      961 gattatactg caggcgattt tttcgatagt gtcagctttc gtggtgtgca gctggcgtca
     1021 gatgacaaca tgctgccaga cagcctgaaa gggtttgcgc ctgtggtgcg tggtatcgcc
     1081 aaaagcaatg cccagataac gattaagcaa aatggttaca ccatttacca aacttatgta
     1141 tcgcctggtg cttttgaaat tagtgatctc tattccacgt cgtcgagcgg tgatttgtta
     1201 gttgaaatca aagaagcgga cggcagcgtc aatagctaca gcgtaccgtt ttccagcgtg
     1261 ccattactcc agcgtcaggg gcgaatcaaa tacgcggtga cactggcgaa atacagaacc
     1321 aatagtaatg aacagcagga gagcaaattt gcccaggcca cgttgcagtg gggcggaccg
     1381 tggggaacga catggtatgg tggtggacaa tatgctgaat attaccgtgc cgccatgttt
     1441 ggtctgggat ttaaccttgg tgatttcgga gcaatttcgt tcgatgcgac ccaggcgaag
     1501 agtacgctgg cagagcaaag cgaacataaa ggtcagtcat atcgttttct gtatgccaaa
     1561 acgctcaacc aattgggcac taattttcaa ttgatgggct atcgctattc gacgtcgggt
     1621 ttctacaccc tttccgacac catgtataaa catatggatg gctacgaatt taatgacggt
     1681 gatgatgaag atacgccgat gtggtcgcgt tattacaatt tgttttacac caaacgtggc
     1741 aaactgcagg tcaatatctc ccagcaatta ggcgagtacg gttcgtttta tttaagtggt
     1801 agccagcaaa cttactggca taccgatcaa caggatcggc tattacagtt tggctacaac
     1861 acgcaaatta aagatctctc gctgggggtt tcctggaact acagtaagtc ccgtggtcaa
     1921 cctgatgctg atcaggtgtt tgcactaaat ttttccctgc cgctcaatct gttgctcccc
     1981 agaagtaatg atagctatac caggaaaaaa attacgcctg gatgacctct aacaccagta
     2041 tcgataacga agggcacatt acacaaaacc tgggtttaac ggagacacta ctcgatgacg
     2101 gtaacctgag ctacagcgtg caacagggat ataacagcga ggggaaaacg gctaatggta
     2161 gcgccagcat ggactacaaa ggggcgtttg cagatgcccg agtgggctac aactacagcg
     2221 ataacggcag tcaacaacaa ctgaactacg ctctttcagg cagtttagtt gcccattcac
     2281 agggcattac cctggggcaa tcgctggggg aaactaacgt gctgattgta gcaccaggcg
     2341 cagagaatac tcgtgtggcg aacagcaccg ggctgaaaac tgactggcgc ggatataccg
     2401 ttgtccccta tgccacttct tatcgggaaa atcgaatcgc acttgatgcg gcatcgttaa
     2461 aacgtaacgt ggatcttgaa aatgcagtag tcaacgtggt tcccaccaaa ggggcgttgg
     2521 ttctggcgga gttcaatgcc catgcgggag caagggtatt aatgaaaaca tcaaagcagg
     2581 gtataccgct gcgttttggc gcgatagcga cgctggacgg catacagact aatagcggca
     2641 ttattgatga tgatggctcg ctctatatgt ccggtttgcc cgctcaggga gcgataactg
     2701 tacgctgggg cgaagctccc gatcaaattt gtcatatcag ttaccagctt accgaacaac
     2761 aaattaactc tgcgattacg cgaatggatg ccatatgcag ataatctttg gagaaaaatg
     2821 cgtgttatta ctacgactat tttttgccgc cgtcttaatg ctatggtgcg ctcaaactgc
     2881 tgcttatatc gggcagtgtc ataccactca ggggaatccg tatattggcg tcaattttgg
     2941 cgttaaaact ctggatgaag aagaaaatac ggctggagta gttaaagaca aattttatca
     3001 gtggaacgaa tcgagtgatt attatgtttc ctgtgattgc gataaagaca atgtcagaag
     3061 tggccgatgg gcattcgccg cggattcacc gttagtctat ttaggcgaca actggtacaa
     3121 aattaatgac tatcttgccg ccaaagtttt attgcaggtt aaaggcagtt ctcctactgc
     3181 ggttcctttc gaaaacgtgg gcacaggggc agatacacga tggcatattt gcgatcccgg
     3241 cggtcaacgt ttaggtggcc agggggctag cggtaatagc ggtagctttt ccctgaaaat
     3301 attgcagccg ttcgttggtt cggtcgtcat tcctcctatg gcgctggcgc gattatttga
     3361 atgctacaac atacccgcag gtgattcctg cacgactact ggtacaccgg ttttagtgta
     3421 ttacctgtct ggtacgatca attcacttgg ctcatgttcc gtcaatgccg gagaaacaat
     3481 cgaggttgat ctgggcgacg tatttgcggc taactttcgt gttgtagggc ataagcctct
     3541 tggggccaga acggcagaac ttgcaattcc ggtcaggtgt aacacgggaa acgcagggtt
     3601 agttaacgtc aatctgagtc tgacggcaac ctcagacccc agctatcccc aggtgattaa
     3661 gacgtcacgt cctggcgtgg gcgtggtggt gaccgatagc cagaacaaca ttatttcccc
     3721 tgctggtggg acactaccgc tctctattcc tgatgatgca gacagtatcg cgcgaatgaa
     3781 tgtctatcca gtcagcacga caggcgtacc accagaaacc gggcgatttg aagccacggc
     3841 aacggtgaga ataaattttg attaacgcgt gaacgtatga agaaaaaaac gatatatcag
     3901 tgcgttattt tgttctttag ccttcttaac atccatgtcg ggatggctgg gcctgaacaa
     3961 gtcagtatgc atatttatgg gaatgtggtc gatcagggct gtgatgtcgc caccaaaagt
     4021 gcattacaaa atattcatat tggtgatttt aatatcagtg attttcaggc cgcgaatacc
     4081 gtaagcactg ctgctgattt gaatattgat atcaccggtt gtgccgctgg tattactggc
     4141 gcgggcgtcc tttttagcgg cgaggctgac acccttgcgc cgacactgct caaactaact
     4201 gacacaggcg gaagcggtgg tatggcaacg gggattgccg tgcaaattct tgatgcgcaa
     4261 agtcagcaag aaatccagct caatcaggtc cagcctctta cgcccttaaa agccggggat
     4321 aacacactca aatatcaact tcgttataag tccacaaagg cgggagcaac gggcggtaat
     4381 gcgacggcgg ttctctattt tgatctggtt taccagtgaa ggagtgatgt tgaaacggat
     4441 aatctggatt ctgttcttac tgggattaac ggggggctgt gagctatttg cccatgatgg
     4501 cacggtcaac attagcggat cgtttcgccg taatacatgc gtgctggcac aggatagcaa
     4561 gcaaatcaat gtgcagttag gcgatgtctc gctgacacgt ttttttcatg ggaattatgg
     4621 cccggagaaa tctttcatca ttaatttgca ggattgcgga acagatgtca gcacggttga
     4681 tgtcaccttt tcaggaaccc tcgacggagt gcagagcgaa atgctgtcca ttgaaagtgg
     4741 tacggatgca gccagtgggc tggcaattgc gattctggat gatgcgaaga tattgattcc
     4801 gcttaaccag gcgagtaaag actacagcct acatagcggt aaggtgccat taacttttta
     4861 tgcgcaattg cgacctgtca atagcgatgt gcagtcgggt aaagtgaatg ccagcgcgac
     4921 atttgtactt cattatgact aatacgtgga acagattagc gctcttgatt ttcgccgttt
     4981 tatcgctgct ggtggcgggt gaattgcagg caggagtggt ggtcggcgga acgcgattta
     5041 tctttccggc agacagagaa tcgatatcta ttttactgac taataccagt caggaatctt
     5101 ggcttattaa cagcaaaatc aatcgcccaa cgcgttgggc cgggggggaa gcgtcgacag
     5161 tgccagcact attactggcc gctccgccac ttattctcct gaagcccggt actacaggca
     5221 cgttgcgctt gctgagaacg gaaagcgaca tcttgcctgt ggatcgcgaa acgctatttg
     5281 agttaagcat tgccagcgtg ccatccggca aagttgaaaa tcagagcgta aaagtggcga
     5341 tgcgctcggt atttaaactg ttctggcgac acgaaggttt gccgggcgac ccgctggaag
     5401 cttatcaaca attacgctgg acacggaatt cgcagggtgt acaactcact aacccaacgc
     5461 cttactacat taacctgatt caggtgagcg taaatggtaa agcgttaagt aatgcgggag
     5521 tcgtgccgcc taaaagccag cgtcagacaa actggtgtca ggtaatagca ccgtgtcatg
     5581 tcgcctggcg gacgataaat gattacggcg ggttgagtgc aaagaaggag caaaatctgc
     5641 cctgaaacag gttctgaaaa cgtttgcgtt ttttttgccg caggtcaatt cccttttggt
     5701 ccgaactcgc acataatacg cccccggttt gcacaccggg aatccaggag agttcatgta
     5761 ctaccccttc gttcgtaaag cccttttcca gctcgatcca gagcgcgctc atgagtttac
     5821 ttttcagcaa ttacgccgca ttaccggaac accgtttgaa gcactggtgc ggcagaaagt
     5881 gcctgcgaaa cctgttaact gcatgggcct gacgtttaaa aatccgcttg gtctggcagc
     5941 cggtcttgat aaagacgggg agtgcattga tgcgttaggc gcgatgggat ttggatcgat
     6001 cgagatcggt accgtcacgc cacgtccaca gcctggtaat gacaagccgc gtctctttcg
     6061 tctggtagat gccgaaggtt tgatcaaccg tatgggcttt aataatcttg gcgttgataa
     6121 cctcgtagag aacgtaaaaa aggcccatta tgacggcgtc ttgggtatta atatcggcaa
     6181 aaataaagat acgccagtgg agcagggcaa agatgactat ctgatttgta tggaaaaaat
     6241 ctatgcctat gcgggatata tcgccatcaa tatttcatcg ccgaataccc caggattacg
     6301 cacgctgcaa tatggtgaag cgctggatga tctcttaacc gcgattaaaa ataagcaaaa
     6361 tgatttgcaa gcgatgcacc ataaatatgt gccgatcgca gtgaagatcg cgccggatct
     6421 ttctgaagac gaattgatcc aggttgccga tagtttagtt cgccataata ttgatggcgt
     6481 tattgcaacc aataccactc tcgatcgttc tctggttcag ggaatgaaaa attgcgatca
     6541 aaccggtggc ttaagtggtc gtccgcttca gttaaaaagc acagaaatta ttcgccgctt
     6601 gtcactagaa ttaaacggtc gcttaccgat catcggtgtt ggcggcatcg actcggttat
     6661 cgctgcgcgt gaaaagattg ctgcgggtgc ctcactggtg caaatttatt ctggttttat
     6721 ttttaaaggt ccgccgctga ttaaagaaat cgttacccat atctaattat ttcttcgaat
     6781 tcctctttat aagataatca gggctttatt ttcagccctg gttgttttat attcatcgct
     6841 gttgcttatt tagacatttt gtacttttat tgttgaggtt attaagcgaa gcaacaatgg
     6901 attgtgttgc tgcggtttta tagggtaggg gagaggcaga tgcgaattaa accagacgat
     6961 aactggcgtt ggtattacga tgaagagcac gatcgaatga tgctcgattt agccaatggt
     7021 atgctatttc gctcacgttt tgcgcgcaaa atgttgactc cagatgcctt ttctcccgca
     7081 ggcttttgcg ttgatgatgc cgcgctctat ttctcttttg aagaaaagtg ccgcgatttt
     7141 aatttatcta aagaccaaaa agccgaactg gtgctgaatg cactggtagc aattcgttat
     7201 ctcaaaccgc aaatgccgaa aagctggcat tttgtctccc atggtgaaat gtgggtgccc
     7261 atgccgggcg atgcggcctg cgtttggttg agcgatactc acgagcaggt caatttgctg
     7321 gtcgttgaat ctggcgaaaa tgccgcgcta tgcctgctgg cacaaccctg cgttgttatt
     7381 gcgggtcgcg cgatgcagtt gggcgacgcc attaaaatca tgaacgacag gctgaaaccg
     7441 caggtgaatg ttgacagctt cagcctcgaa caggcagtct aacgcgtcag cttaagggca
     7501 gtcttcggta cacagctaca gcaaagaatg gtgccatcat cgcccattgc tgattttttc
     7561 agcggcgtga cttcgccttc taaaagctga acacggcaac ttccacaaat gcccgcgcgg
     7621 caagaataag ggatacgaat tccctgattt tctaattgct ccagcaacac ttgttggtta
     7681 tttccacgaa atgcctgtcc ctgccagtca atatctacat ttgcgtccgg ttgttgcgtg
     7741 atgttggcgg tatcatcagc ggcagctgcg ccgtaaattt ttgccggagc cgttgccaga
     7801 atttccacct catcgccaac gcgaatcacg ccgctattac gggcaattaa attctgacca
     7861 aaatcgacat cgccgttatc ctgggcagtg cggaaagatt gcaatgtttt taatggttcg
     7921 cctgccggat gtttttgccc tttttctggg ctgacggtgg tgaaaataca gcggctacaa
     7981 ggtttaacca catcaaacac cacatcacca atgcgaatca ctttccagct atcttcttcc
     8041 caggctgacg cgccggaaac caccagattg gggcggaatt gctccatttt tacactggcc
     8101 ggacaacgtt gttggagatc acgtaacgag gcttcgttag caagaaggta aggatagcca
     8161 tcagcaaatg acagaggtac agtgttgtgg cgtttcacgc gccgggtcat ttgtggcccc
     8221 acccagcgta attgcacttc gcgggagaaa aatccactta gccatttgtt gatcgcgtct
     8281 ggcgcaattc gcgcggtaaa atgtgtgccc caaacttcgg ttggtgcgtc ttgtgtggcg
     8341 aaatcagcaa aacgaacata tgcgctactg ccatctggtg cggtgagatg caagccatca
     8401 tgcacaggtg aaggagtaaa ccgtaccatc tggggaaact ggcgagcggt aataaacgta
     8461 ccgtcaggtt ccgtgatcat aaagatgcga tcgaaggcca gaccactgac atcagccaga
     8521 gcatgtgtaa gaccaatgcc gcgcatcgat ttaacaggat gaataaaaag ccgggttaat
     8581 gtcgccacgg tgcggtcctc aaatgaaaat aagccctcaa ctttatgaca tgacgcgctt
     8641 attagctata atgcgcaaca attttcttag cgcctgtccc gttaggctat tttatttgtc
     8701 agtttggccc tgggttgtgc tcgaaatcct cacgtacttg tgtacgctcc ggtttctccg
     8761 cgctctccat gtccagactg actgcaacaa ttacgcctac tgcggtaggc tcttagagta
     8821 aaagtgacga tatgaattct ctgtttgcca gtacggcccg tgggctggaa gagctgttaa
     8881 aaactgaact ggaaaatctg ggggccgttg aatgccaggt ggttcagggt ggggtccatt
     8941 tcaagggcga cacacggctt gtttaccaga gcctgatgtg gagccgcctg gcctcgcgta
     9001 ttatgttgcc gctgggcgag tgtaaggttt acagcgattt agacctctat ctcggtgttc
     9061 aggcgatcaa ctggacagag atgtttaatc ctggcgcgac cttcgctgtc cacttcagtg
     9121 gtttgaatga caccatccgc aacagtcagt acggcgcgat gaaagtgaaa gacgcgatcg
     9181 tcgatgcttt cacgcggaaa aatctgccgc gtccaaatgt tgatcgcgat gcgccggata
     9241 tccgcgttaa cgtctggctg cataaagaaa ccgccagtat cgctcttgat ctgagtggtg
     9301 atggtttaca tctgcgtggc tatcgcgatc gtgctggtat tgcgccgatc aaagaaaccc
     9361 tggcagccgc tattgtgatg cgatccggct ggcagtcagg aacaccgctg ctcgatccga
     9421 tgtgtggttc cggtacgttg ctgattgaag cggcgatgct ggcgaccgat cgcgcaccag
     9481 gcttgcaccg tgggcgttgg ggctttagcg gctgggcgca gcatgatgaa gctatctggc
     9541 aggaagtgaa agcggaagcg caaactcgcg cccgtaaagg cctggctgag tatagctctc
     9601 atttttacgg ttcggacagc gacgcacggg tgattcaacg tgcgcgcact aacgcccgtc
     9661 ttgcggggat tggtgaactg atcacctttg aggtgaaaga tgtcgcgcaa ctgaccaatc
     9721 cgctgccgaa agggccgtac ggtacagtgt tgagcaaccc gccatacggt gaacgtctgg
     9781 acagcgaacc ggcgctgatt gcgctgcata gtctgctggg ccgaatcatg aaaaaccagt
     9841 tcggtggctg gaatctctct ttgtttagtg cctcgccgga tctgctaagc tgcctgcagc
     9901 tgcgtgcaga caaacagtac aaggcgaaaa acggcccgct ggactgcgta cagaaaaatt
     9961 accacgttgc cgaaagcaca ccagacagta aaccggcaat ggtagcggaa gattacgcca
    10021 accgcctgcg taagaacctc aaaaaattcg agaagtgggc acgccaggaa gggattgaat
    10081 gttaccgcct gtatgacgcc gatctgccgg aatataacgt tgccgttgac cgttatgccg
    10141 actgggtggt ggtgcaggag tatgcgccgc caaaaactat tgatgcgcac aaagcgcgtc
    10201 agcgtctgtt cgatattatc gctgcaacca tttcggtact ggggattgcg ccaaacaaac
    10261 tggtgctgaa aacccgtgaa cgccagaagg gcaaaaacca gtaccagaaa ctgggcgaga
    10321 agggcgagtt tcttgaagtt accgaatata acgctcactt gtgggtgaac ctgacggatt
    10381 atctcgatac tggtctgttc ctcgatcacc gtatcgcccg ccgtatgctc ggtcagatga
    10441 gcaaaggcaa agatttcctc aacctgtttt cttataccgg cagcgccacc gtgcatgcgg
    10501 gattaggcgg tgcacgcagc accaccaccg tggatatgtc gcgtacttat ctggaatggg
    10561 cagaacgcaa cctgcgtctg aatggcctga ctaggcgtgc gcatcgcctg attcaggccg
    10621 attgcctggc gtggctgcgt gaggcaaatg aacagttcga tctgatcttt atcgatccgc
    10681 caaccttctc taactcaaaa cgaatggaag atgcgtttga tgttcagcgc gatcatctgg
    10741 cgctgatgaa agatttgaaa cgtctgctgc gtgcaggtgg gacgatcatg ttctcgaaca
    10801 acaaacgtgg cttccgtatg gatctcgacg gcctggcaaa actgggactg aaagcacaag
    10861 aaattacgca aaaaacgctc tcccaggatt tcgcccgtaa ccgccagatc cacaactgct
    10921 ggctgattac cgcagcctga aaggaatagt aatgtcatta atcagtatgc atggcgcatg
    10981 gctgtcgttc agcgacgcgc cgcttctcga taacgcagaa ctgcatatcg aagataacga
    11041 acgtgtttgt ctggtgggcc gcaacggcgc aggaaaatcg acgttaatga aaatcctcaa
    11101 ccgtgaacaa gggctggatg acggtcgcat tatttatgag caagatttga ttgtagctcg
    11161 tctgcaacag gatccgccgc gtaacgttga gggtagcgtt tatgatttcg ttgccgaagg
    11221 cattgaagaa caagcggaat atctgaaacg ctatcacgat atttcgtgcc tggtgatgaa
    11281 cgacccgagc gagaaaaatc tcaacgaact ggcgaaggtt caggaacagc tggatcacca
    11341 caacctgtgg cagctggaaa accgcatcaa cgaagtgctg gcgcaactgg ggttagatcc
    11401 taacgttgcg ctgtcgtcgc tttccggcgg ctggttgcgt aaagcggcat taggacgcgc
    11461 gctggtgagc aatccgcgcg tattgctgct ggacgaaccg actaaccatc tggatatcga
    11521 aaccatcgac tggctggagg ggttccttaa aaccttcaat gggacgatta ttttcatctc
    11581 ccacgaccgt tcgtttatcc gcaatatggc gacgcgcatt gttgatctcg atcgcggcaa
    11641 gctggtgacc tatccaggga attacgacca gtacctgcag gaaaaagaag aagccctgcg
    11701 cgtggaagaa ctacaaaatg ccgagttcga tctcaaactg gcgcaggaag aagtgtggat
    11761 tcgctagggg atcaaagcac gccgtacccg taatgaaggc cgcgtacgcg ccctgaaagc
    11821 gatgcgtcgc gaacgtggtg aacgtcgcga agtgatgggt accgcaaaga tgcaggtgga
    11881 agaggccagc cgctccggta agatcgtttt cgaaatggaa gacgtttgct accaggttaa
    11941 cggtaagcaa ctggtgaaag atttttctgc ccaggttcta cgtggcgaca aaattgccct
    12001 gattggtccg aatgggtgcg gcaaaaccac gctgctgaaa ctgatgctcg gtcagcttca
    12061 agcgggcagc gggcgtattc acgttggcac caaactggaa gtggcttatt tcgatcagca
    12121 ccgcgcggaa ctggatcccg ataaaacggt gatggataac cttgccgaag gtaagcaaga
    12181 ggtgatggtt aatggcaagc cacgccacgt attgggctat ttgcaggact ttctgttcca
    12241 tccgaaacgg gcgatgacgc cagtacgtgc gctttctggc ggtgagcgga accgcttgct
    12301 gctggcgcgt ttgttcctca aaccaagcaa cttattgatt cttgacgaac cgaccaacga
    12361 tcttgatgtc gaaacgctgg aactgctgga agaactgatc gacagctatc agggcacggt
    12421 attgctggtt agccacgatc gtcagtttgt cgataacacc gttacagaat gctggatctt
    12481 cgaaggcggc ggtaaaattg gtcgttatgt tggcggttat catgatgccc gtggtcagca
    12541 agaacaatat gtggcactca aacagcctgc ggtgaaaaaa agcgaagaag ccgccgcgcc
    12601 aaaagcagaa actgtaaaac gcagcagtag caaactaagc tataaattgc agcgcgaact
    12661 ggagcagcta ccgcaattgc tcgaagatct ggaggcgaag ctggaagccc tacagacgca
    12721 agtggcggat gcttccttct tcagtcagcc gcatgagcag acgcaaaaag tgcttgctga
    12781 tatggctgct gcagagcagg agctggagca agcctttgaa cgctgggagt atcttgaagc
    12841 gttaaaaaat ggtggctgat cgcaaaatag tcgatttaaa cagagacagc tgacaacggt
    12901 aaaattgcct gatgaactgg gtttatcagg catgaaaacc aacactttgt tagtcatctg
    12961 taaaatagcg catcattaag gagtaccaat gtgcgaacat catcatgccg cgaagcacat
    13021 cctgtgctcg cagtgtgaca tgctggtggc gttaccgcgc cttgagcatg gtcagaaagc
    13081 ggcatgtccc cggtgtggca caacgttaac cgtggcgtgg gatgcccctc ggcagcgtcc
    13141 caccgcctat gcgttggctg cactgttcat gctgttgctg tccaacttgt ttccttttgt
    13201 gaatatgaac gttgcaggag ttaccagtga aattacatta ctggaaattc ccggcgtgct
    13261 tttttctgag gactacgcca gcctcggcac ctttttcctg ttgtttgtgc aactggttcc
    13321 cgcgttttgt ctgataacca ttctgttact ggtgaatcgc gcggaattac cggtccgttt
    13381 aaaagagcaa ctggcacggg tgctttttca actcaaaacc tggggaatgg cggagatttt
    13441 cctcgcgggt gtgctggtca gtttcgttaa actgatggct tacggcagca ttggggtagg
    13501 cagcagcttt ctcccctggt gtttattttg tgtcctgcaa ctgcgcgctt ttcagtgcgt
    13561 tgatcgtcgc tggttatggg acgacatcgc cccgatgcca gaactgcgcc agccgctaaa
    13621 accaggcgtc acggggatac gtcaggggct gcgttcgtgc tcctgttgta cggcaatcct
    13681 tcctgctgat gaacccgtgt gcccgcgttg tagtaccaaa gggtacgttc ggcgtagaaa
    13741 cagcctgcag tggacactcg cgctgcttgt aacgtccatc atgctgtatc ttccggctaa
    13801 tattttgccc atcatggtga cggatttatt aggctcgaag atgccgtcga cgattctcgc
    13861 tggggtcatt ctgttatgga gcgaaggctc ttatcccgtc gctgtggtga tctttctggc
    13921 cagtattatg gtgccaacgt taaagatgag cgccatcgcg tggctgtgtt gggatgccaa
    13981 agggcatggc aagcgcgaca gtgaaagaat gcatttgatt tatgaagttg ttgagtttgt
    14041 aggccgctgg tcgatgattg acgttttcgt tatcgcggtg ctctcggcgc tggtgcgtat
    14101 gggaggttta atgagtattt atccggcaat gggtgcatta atgtttgctt tagtcgtcat
    14161 aatgacaatg ttttctgcta tgacgtttga cccgcgtttg tcgtgggatc gtcaacctga
    14221 atcagagcat gaggagtcct gacagtatgg aatctaataa tggggaagcc aaaatccaga
    14281 aagtgaagaa ctggtctccc gtgtggatat ttcctatcgt cacggcgctc attggggcct
    14341 gggttctttt ttatcattac agccatcagg gaccggaagt gaccctgatc accgcgaatg
    14401 cggaaggaat tgaaggtggc aaaaccacca ttaaaagccg tagcgttgac gtcggcgtgg
    14461 ttgaaagcgc cacactggct gatgatttga cgcacgttga aatcaaagcg cggctgaatt
    14521 ccggtatgga aaaattgctg cataaagaca ccgtcttttg ggtggtgaaa ccgcagattg
    14581 gtcgcgaagg gattagcggc ctgggaacgc tgctgtctgg agtttatatc gaactgcagc
    14641 caggcgcgaa aggcagcaaa atggataaat acgatttgct ggactcacca ccgttggccc
    14701 cgcctgatgc gaaaggtatc cgtgtgattc tcgatagcaa aaaagccggg cagctctcgc
    14761 caggagatcc ggtgctgttc cgtggctatc gggtaggttc ggttgaaacc agcaccttcg
    14821 atacgcaaaa acgcaatatc agttatcaac tgttcatcaa tgcaccttat gaccgactgg
    14881 tgaccagcaa tgttcgcttc tggaaagata gtggcattgc ggttgatctg acgtcagcgg
    14941 ggatgcgtgt ggagatgggc tcattgacaa cgctgcttag tggcggtgtt agctttgatg
    15001 tgccggaagg tctggattta gggcagccag tggcaccgaa aacagctttc gttttgtatg
    15061 atgatcagaa gagcattcag gattcgttgt acaccgatca cattgattat ctgatgttct
    15121 ttaaagattc ggtacgcggt ctgcaaccgg gagctccggt agaattccgg ggtattcgcc
    15181 tgggtaccgt aagcaaagtg ccattctttg cgccgaatat gcgtcagaca tttaacgatg
    15241 attaccgtat tccggtactg attcgtatcg agccagagcg gctgaaaatg cagcttggcg
    15301 aaaatgcgga tgttgttgag caccttggcg aattgttgaa acgtggttta cgcggatcgc
    15361 tgaaaaccgg aaacctggtc actggtgcac tgtatgttga tctcgatttc tatccaaata
    15421 cgcctgcaat aaccggtatt cgtgaattta atggttatca gattatcccg actgttagcg
    15481 gcggcctggc gcaaatccag caacgactga tggaagcgtt ggataagatc aacaaactgc
    15541 cattgaatcc gatgattgaa caggcaacca gtacgctttc tgaaagtcag cgcacaatga
    15601 aaaacctgca aacgacgctg gatagcatga acaagatcct cgccagccag tcgatgcagc
    15661 agttgccgac ggatatgcag tcaacgttgc gtgaattgaa tcgcagcatg cagggcttcc
    15721 agcctggctc cgcagcctac aacaagatgg tggcggatat gcagcgcctt gatcaggtgt
    15781 tgcgagaact gcaaccggtg ctgaaaacgc tcaatgagaa gagtaacgcg ctggtatttg
    15841 aagcgaagga caaaaaagat ccagagccga agagggcgaa acaatgaaaa agtggctagt
    15901 gacgattgca gcactgtggc tggccggatg cagctccggc gaaattaata aaaactatta
    15961 ccagttacct gtggtgcaga gcggtacaca aagtaccgcc agccagggca atcgtctgtt
    16021 atgggtagag caggtcactg ttcctgacta tctggcgggg aatggtgtgg tttatcaaac
    16081 cagtgatgtg aagtatgtga ttgccaacaa caacttgtgg gccagcccgt tggatcaaca
    16141 gttgcgcaac accctggttg ccaacctgag tacgcaactg cccggctggg tggttgcctc
    16201 ccagcctctg ggaagcgccc aggacacgct caatgttacc gtaacggagt ttaacggtcg
    16261 ctatgatggc aaggtcattg tcagtggtga gtggctgttg aaccaccagg gacaactgat
    16321 caaacgtccg ttccgtctgg aaggagtgca aactcaggat ggttacgatg agatggttaa
    16381 agtgctggcc ggtgtctgga gtcaggaagc cgcttctatt gcacaagaga taaagcgtct
    16441 accttaatta taaagatttg taaatataac cgtctccggt atgccgcctg aggcggtttt
    16501 tttgtttcta acgtgcggaa aaatttgttc ctcttcacat tttttgtaca accgacatgc
    16561 ccgtgtagct cacaaatatg acagtggcgt gaattttgcg cattgacggc agttatgatt
    16621 cgcggtattg cttaactgtg attgcacatt tagtaatcac tgttttcttt tccaccagaa
    16681 accagtatga gggaaacgag gcatgaagag acaaaaacga gatcgcctgg aacgggcaca
    16741 tcaacgtggt tatcaggccg gcatcgccgg acgctcaaaa gaaatgtgtc cctatcagac
    16801 gctgaatcaa aggtcacaat ggctgggagg ctggcgagaa gccatggcgg acagggtagt
    16861 aatggcctga ttctgtctct ttaaaaagaa acctccgcat tgcggaggtt tcgccttttg
    16921 atactctgtc tgatgataat cagaaggcag acgtatcctg gaacagaccg actttcaggt
    16981 cgctggcggt atagatcaga cgacaatcaa ccagcacttc gccatctgcc agacccataa
    17041 tcagacgacg attaacaatg cgtttaaagt gaatacggta ggtcactttt ttcgctgtcg
    17101 gcagtacctg accagtgaat ttcacttcgc caacgcccag cgcgcggcct ttaccttcgc
    17161 cgcccagcca gccgaggtag aaccctacca gctgccacat tgcgtccagg cccaggcatc
    17221 ccggcataac cggatcgcca atgaagtggc atccgaagaa ccacagatcc ggattgatat
    17281 ccagttctgc ttcaacatac cctttgtcga agttaccacc cgtttcggtt attttgacca
    17341 cacggtccat catcagcatg ttcggtgctg gcaattgcgg gcctttagcg ccaaacagtt
    17401 caccgtgacc agaggcaaga aggtcttctt ttgtatagga ttcgcgttta tctaccatgt
    17461 tctctgtaag ccttatttta ttgaagcacg caggatagct aacacgtgta cgctgaacaa
    17521 gtccgatcag ttcggaataa accagttcag ccaacgtaat ggccatggaa aacggtgacg
    17581 tccttcctgt tgcgatgctt gcgcgatacg ttcctggatg gtttgcatca gcgtcgtttg
    17641 gccttcgcca tcccacacca gatttaataa taacggcagt gcgtcagtca catcgtctac
    17701 tgcccagatg gtgaatttgc cttcttctac cgctttcacc agttcactgt gaagacttaa
    17761 atggcgaacg ttagcagtgg ggataatgac accttgtttc cccgttaact cacgttgctg
    17821 gcaaatagca aagaagcctt cgattttctc atttaaacca ccgaccggct ggacgcggcc
    17881 gaactgatcg actgaacctg tgatagcgat actctgattc actggcacat cagcgagggc
    17941 gcttatcagg gcgcagagtt cagccatcga ggcactatcg ccatccactt cactgtatga
    18001 ctgctcaaat gtcagcgatg ctgagaaggg gatctgttgc tcaagctgta gttccgacat
    18061 caggaacgcc tgcatgatca tcatcccttt cgcatggata ttgccgccaa gctccgcttt
    18121 gcgttcgatg tcggtgaatt caccatcgcc aatatgcaca acgcagctaa tgcgagaagg
    18181 ttcgccaaaa gcgcgtggat gacccggaaa ttcaatgacc gaaagggcgt taatttgccc
    18241 gatgcgttcg ccttcggttt caatcaggat ttgctcctga aggatctcat cctgcatacg
    18301 ttcagcgagg aaaccttcgc gccattcacg ctgctgcagc attaaattta gctgctcgcc
    18361 ggagaaggtg tcgccatcac acagggaggc gacctcttta cactggcgga ggatccaatg
    18421 cgggctaagc ggaagtgttt cttgttcacc ggtgtagcgt gctgcttcgc ggataagtac
    18481 cggccaagca tccgctcccg gggcaggtaa gtgattatgt ctggcggtaa atgtcaccca
    18541 gcgacaccac tggcttactg actccgcatc gacaatctgc agagtatctt caaattcgct
    18601 ataaatagcc tgctctgaaa gctctggctc catctcctgg aaatcagcca atgattcgcg
    18661 ttcgcctacc agaatgactt tcagcttcaa tggcatcgaa ggcacagaga cggggagagg
    18721 gcgcgactca tcaaacgcaa cccagtcaaa acgctcgcgg ttaacgatat ttttcagccg
    18781 catccacagc agaggttgcg ccagcagtgt acgcaaagag atgatgagaa taccgccatt
    18841 tgcctgatgc accagaccag gctgcagggt aatgtcgcca ttaaactggc gcaggcagcc
    18901 aaagagttgc tccgcttcta cccagtcggc agcgacaact tgcgttaaag tcgcaaaatt
    18961 atcatctgca ctcactgcgt gacgtaagcg gatggagtgg ccggaaacct catagtgatc
    19021 gcccaccagt tggcctgcat cgctttgtag cgtacgcgcg gcatcggcaa taagattcag
    19081 atactcagac tcttccgggg ccttagccag cataaaagag gaggatgctc gcgtatgcag
    19141 caactgctcc agcgcaaatt gcagtcgcgg ttgagtatca ctgaataaag gatcgttttc
    19201 gtcaatcaaa tgtggctgag caaatatttc ctgatagcta tcggtatcag gaaccaggtc
    19261 acgccatgca agtttcgtaa tggtcaaagt tgatgttttt tagtctgttg tcaaagccgc
    19321 gattataccg taaccggcac tacagcacac gtagaaagca ccgacaatac tcctggctgg
    19381 gcgttaaagc tcacaggatg gagattcttt tcttcactgg cctaaaaagc tgatattctg
    19441 taaagagtta cacggtaaca ttgagatcgc tatgaaatat caacaacttg aaaatcttga
    19501 aagcggttgg aaatggaagt atctggtaaa gaagcatcgc gaaggggagt taattacccg
    19561 ttacatagaa gccagtgccg cccaggaagc cgtcgatgtg ttgctctcgc tcgaaaatga
    19621 acccgtgctg gtaaatggct ggattgacaa gcatatgaat ccggaactgg tcaatcgcat
    19681 gaagcagacc attcgggcca gacggaagcg ccattttaat gcggaacacc agcatacgcg
    19741 caaaaaatcg atcgatctgg aatttattgt ctggcaacgt ctggctggtc ttgcacagcg
    19801 tcgcggtaaa acgctttctg aaacgattgt tcagctgatt gaagatgcgg aaaacaaaga
    19861 gaaatacgcg aataaaatgt cttctctgaa gcaggatctg caggcattgc tgggtaagga
    19921 ataactgacg aaagtcagtt caatttacta aaggcaaaaa aaaccccgca gcagcggggt
    19981 ttttctacca gacgataact taagcctgcg gctgagttac aacgtctttg atacctttaa
    20041 cttcgatctc tacgcgacga tccggagcca agcagtcgat cagtgcagca cgctgtttca
    20101 cgttgtcaca ggtgttgcca gtaaccgggt tggattcgcc cataccacgt gcggagatct
    20161 tgtctgccgg gatacctttg gagatcaggt aatcaacaac agactgagca cggcgctcgg
    20221 acagaccctg gttgtaagcg tcagaaccga tgcggtcagt gtaacccaga acaactacgg
    20281 aaccgtcttt cggatccagg ttgctcagct ggctgtacag ctgatccaga gcagcctgac
    20341 cttccggttt cagggttgct ttgttgaagt tgaacagaac gtcagacttc agagtgaagt
    20401 gcttggtctg tacttccggt gccggagctg gagcaactac cggagctgct tcgccctgac
    20461 cgaaacggta ggaaacaccc aagctcagca gaccgttgtc cggacgagta ccgatggtgt
    20521 ttgcgtcacc gatgttgttg gtccactggt attccagacg ggtagcgatt tcaggagtga
    20581 tcgcgtactc aacaccacct gcgaaaaccg gagaaacgcc agtgtcgtgg tctttaaagg
    20641 atgcgccacc aggtacgtta gccttggtgt ctgcacgcca taccatacca cccagacgag
    20701 tgtagatgtc cagatcgtca gtgattgggt aacccagttt agcggtcagc tgaacgccct
    20761 gagctttgta tgcgccgttg atgttgtcgc ctttgtacgg catacgacct aaccagtcgt
    20821 aacccatttc aaaaccaaca tacgggttaa cctggtaacc accaaaagca cctgcaccca
    20881 gttggttttc gtgggtcgga ccattgttag gaataaaacc agtgtcatgg tactgggacc
    20941 agcccagttt agcaccagtg taccaggtgt tatctttcgg agcggcctgc gctacggtag
    21001 cgaaaccagc cagtgccact gcaatcgcga tagctgtctt tttcattttt tgcgcctcgt
    21061 tatcatccaa aatacgccat gaatatctcc aacgagataa cacggttaaa tccttcaccg
    21121 ggggatctgc tcaatattaa ctctaccgat atcttcggct tatgccgagc acccctggcg
    21181 atgtaaagtc tacaacgtag ttgaaaactt acaagtgtga actccgtcag gcatatgaaa
    21241 aaaaagtctt gtataaggta tgtttaatct ttttgtcagc gacaatttac agaaaaaaaa
    21301 cgcggaaacc gcttcagaca agccttcgca aggaaaatta gtcacgactg aaagcattgg
    21361 ctgggcgaca aaaaaagttc caggattaat cctaaattta cttaatgata caaattagag
    21421 tgaattttta gcccggaaag ttgtctcgtt gtgtgagagg atgcgcttac cggacgcata
    21481 ataaacccca tagcgttacc ttcatttgcc gcatcaacaa gttcagcatg ctcttcttca
    21541 gtcaaatcat ctgtcaacca accgatcacc acactgtaat tgcccgtgcg taaagcgcga
    21601 accattgact ccactgtgtg gcaaggggag agctggctaa tctgcattac tttcgttaag
    21661 ggtagcccag atgcctgaac ccattcccga ctcagttttt gttgcggtgt taaccagagt
    21721 tgccagcgcg attgctgacc gagttgctgt aacaatggca acagtagaag ttgcgtcatc
    21781 atgggctgat cttcgcgata gacaacttca ctgataagcc cggctgtagt gttttccgta
    21841 gagacacgcg caattttact tgctgcggat gagaacgacg aagaacgatg tgcatagcct
    21901 gaagtgtaca taatcaatcc agcccctgtg agttactgta tggatgtgca gtacatccag
    21961 tgacaacaaa gatcaaccct attttcggaa agagcctcgc aaattttgtc gttggtgacg
    22021 ggaaaacata aattaatctt gccccttaag aataagttgc ctattttcgt agttaacgga
    22081 tccgttaatg tgaatcattc ttttatgtta tgattttaaa aggaatttta tgaaaagcct
    22141 ctcctataag cggatctata aatcacaaga atacctggca acgttgggca caattgaata
    22201 ccgatcattg tttggcagtt acagcctgac cgttgacgac acggtgtttg cgatggtttc
    22261 tgatggtgag ttgtatcttc gggcttgtga gcaaagtgca cagtactgtg taaaacatcc
    22321 gcctgtctgg ctgacatata aaaagtgtgg ccgatccgtt accctcaatt actatcgggt
    22381 tgatgaaagt ctatggcgaa atcaactgaa gctggtgcgt ctgtcgaaat attctctcga
    22441 tgcagcgctg aaagagaaaa gcacgcgcaa tacccgggaa agactgaaag atttgcccaa
    22501 tatgtctttt catctggaag cgattctcgg ggaggtgggg attaaggatg tacgggcgtt
    22561 acgtatactt ggggcaaaaa tgtgttggtt gcgactgcgg cagcaaaaca gtctggtgac
    22621 agaaaagatt ctgtttatgc ttgaaggtgc cattatcggc attcatgaag ctgcgctccc
    22681 ggtggcacgc cgccaggagc ttgcagaatg ggctgactct cttacgccga aacaggagtt
    22741 tcctgcggag cttgagtaat ctggcgttgc agacgaccaa tctctggcag taatgcaatc
    22801 aataacccaa cttgttgtac gaccagaggt tctttgctat ctgcccgtgg ttcaagttgc
    22861 tgcatccgct gtttcaggcc agctaatgcc tgattgacgc gttcttcatc agcaggttga
    22921 tgatgtaacg cgtcatcaac atagcaaact gcgtcatcaa gaaacgccag aatttcagga
    22981 ttagttaact gctcccggtg agcaccgagg gctgagatat agctggtaaa cgtatggtta
    23041 aggcacagca agcgaaacgc ggcttcgcga atttgcgggg taacgttcgg ttcgctggac
    23101 atatttgata ccacagacgc cagttcagca tcacggttgt gtgcatcgcg gcgggcaata
    23161 cgatacgcca gacggttatc acgcccctga tggtattgct ccagtatggc atcgagatag
    23221 cgacagttgg cctctgtggc gcgttcgagc attcgcggca gattgcgaaa cttccagtcg
    23281 ggccagatgt agctcactgc cgcccacgca atggcacaac caatcagcgt atcgattacg
    23341 cgaggtaacg ctacttcaaa accttcaccc agtaagttaa aacacagtag caccaaaagt
    23401 gtgatgaaca tcgttgcatg agcgtattgc acgttacgga aggcaaaaaa gagcacgccg
    23461 gtaataacca gcagcaccag ctgcccttcc agtgatggca caaaccacag cacaggaatg
    23521 ccaatcgcga tacctaccag cgtaccaata atccttaact tcaggcggtg gcgcgtggcg
    23581 ttatagtttg gctggcagac aaacaaactt gtcagcaaga tccaataccc gtgatgcatt
    23641 ccggttattt gaatgatggc gtagccgaag cacaacacca gcgacattct taccgcatga
    23701 cggaagaggg cggattccgg cgtgaagtga cggctaagac gcagccagat atcactcaat
    23761 ccgtgcgggc tgtcatcagc gagctcattt tcgtcattat tacggggtag tgcctgggcc
    23821 tgttctgatt caattgttgc cagttgggca tcaatggcgc gtaaattgtt cagcaaaaat
    23881 cccagtgttt tgagtaaatc ggcgggtgcg ccgttatcgc gtatccgctc cagcgcagca
    23941 tcaatatgcg taaaagcgcg ctcaaaatgc ggatcatgtt gataagactg acgcaacaaa
    24001 atacagcgtg acagttgctg gcacgcctgg ccctgcatcg acatcagccg ctgaaaacgg
    24061 aacagcacgt cgctgtggcg aaaatgttca cgcaatgttt gatactgaat atgagaagag
    24121 ctggcacgct cgtgaatatc ctgtgcgaca aagtaataat gcagcgtgcg acgcgttccc
    24181 cgttgaccac gatcgccacg taagcgggtc agcagcgaga gtttcgtctg attcaatgtc
    24241 gccatcagct gaccgttggc gagagccaaa tcgtacagcg gtgcctggct ttcatcttca
    24301 atatcaggat caaacatgcg cgacttgagc tcaagataac gcgccagttg ttcatagcaa
    24361 cgcgccaggt tgtcctgcag cgggcggacc gggaacagca gatgaccaat aagtgtcagg
    24421 acgttgtacc agacggcacc ggccagcaga tacatcggct gctgatacca gtgctcatac
    24481 agtgatgttc ccaacatagt gtaaatggcg atcagcaatg caccgaaggc aattgttgca
    24541 tagcgttgac ccagaccgcc gagcaaaatg aagccgctgg tagagagcgt taagccaatc
    24601 gcaaatagcc agggccaggg aaacagcaat tctactgagg ccgaggcgat aaaaaagcag
    24661 aacagcgtaa tgatgaggtt acgcaaacgt cccgccagtc ggtcatcgag atcggtcagc
    24721 gccgctgcca ccatccccag cgttagcgga atcgtcagtt ttacatcacc cagccaccac
    24781 ggaaacgctg tggttccaca aagcgcaata aaaatacgcg cgtaatacag ccaggcgctg
    24841 ttccaagtat agcgtttgag caaaggactt agcataaagg ccatattagt ggattattca
    24901 aaacgacgac gtgcattggc ttcacgcgca gcttgcgctg tttctaccga taccacgcga
    24961 cgaccaaccg gccatagtgc aatagcggca attttaaagt tcgcaatgcc gacaggaatg
    25021 ccaatgattg aaatacattg tgcgatgccc gttgcaatgt gcatcaggca taaccaccag
    25081 ccaaagaaaa tcagccagaa aatattcaat accgtaccgc cagtattcag caacacattt
    25141 ttgccagccg ggttcagttc atcgacatga atagcttcat tgccataagg caccagagac
    25201 agtttagtga tctcccagca ggatcgtgtc agcggtaagg taaaaatcag cacaatactg
    25261 accagagtcg ccaacagcca gcccagagtg gtggcaaatc cgccaagcac aaagttcaga
    25321 atgttcaaaa cggtacgcat aaaacctcgc tttactgtgg ttttcagtaa tgggcggcaa
    25381 ttgtaacgtt tttttgggct ggagcacgtt ttctctgacg gttacactga taagaaataa
    25441 tttcgtgtgg atctacagag tcatggaact gaaagcgaca acgcttggaa aacgtctggc
    25501 acagcaccct tacgatcggg cggtgatcct caatgccggg attaaagtct ccggcgatcg
    25561 ccacgaatac cttattcctt tcaatcaatt actggcgatt cactgtaagc gcggtctggt
    25621 atggggcgag ctggaatttg tactgccgga cgaaaaagtg gtgcgtctgc acggcacaga
    25681 atggggcgag acgcagcgtt tttaccatca tcttgatgct cactggcggc ggtggagtgg
    25741 cgagatgagc gaaattgcgt ctggtgtttt acgccagcaa ctggatttga ttgccacgcg
    25801 cactggagaa aataaatggc tgacgcgtga gcaaacctct gacgttcagc aacaaatccg
    25861 ccaggctttg tcggcgttgc cgttgccggt taaccgactg gaagaattcg ataactgccg
    25921 tgaggcgtgg cgtaaatgtc aggcttggtt gaaagatatt gaaagcgctc ggttgcagca
    25981 taaccaggcg tataccgaag ccatgcttac cgagtatgcg gattttttcc gccaggtcga
    26041 gtcttcaccg ctgaatccgg cgcaggcccg ggcagtcgtt aatggcgagc attctctgtt
    26101 agtgctggcc ggtgcaggaa gcggaaaaac gtcggtgctg gtggcccgtg caggctggtt
    26161 gctggcgcgt ggtgaagcgt cccctgagca aattttattg ctggcgtttg gtcgcaaagc
    26221 cgctgaagag atggacgagc ggattcgcga acggctacat accgaagaca ttaccgcacg
    26281 cacgtttcat gcgctggcgc tgcatattat tcagcagggc agcaaaaaag ttccgatagt
    26341 cagcaaactg gaaaatgata ccgctgcccg tcatgaactc tttattgctg agtggcgcaa
    26401 gcaatgcagc gaaaagaaag cgcaggcgaa gggctggcgg caatggctga cggaagaaat
    26461 gcagtggtca gtgccagaag gtaacttctg ggatgatgaa aaattacagc gtcgccttgc
    26521 ctctcgcctc gatcgctggg taagtctgat gcggatgcac ggtggtgcac aggcagaaat
    26581 gattgccagt gcacccgaag agattcgcga tctgttcagt aaacgtatca agttgatggc
    26641 cccgttatta aaagcctgga aaggtgcgct gaaggcagaa aacgctgtcg atttttcggg
    26701 ccttattcat caggcgattg tgattctgga gaaaggccgc tttatcagcc cgtggaagca
    26761 tattctggtt gatgaatttc aggatatctc gccgcagcgc gcagcgttgt tagcggcatt
    26821 acgcaagcaa aacagtcaga cgacgttgtt cgccgttggt gatgactggc aggcgattta
    26881 ccgattcagc ggtgcgcaaa tgtcgctcac caccgctttc catgaaaact ttggtgaagg
    26941 cgatcgctgt gatttagaca cgacttaccg ttttaacagt cgtatcggtg aggtggcaaa
    27001 ccggtttatt cagcagaacc caggccagct gaaaaagccg ctaaacagct taaccaatgg
    27061 agacaaaaaa gctgtcacgt tattggatga gagtcaactt gacgctttgc tggataagct
    27121 ctctggttat gccaaaccgg aagagcgcat tctgatcctg gcgcgttacc atcacatgag
    27181 gcctgccagc ctggaaaaag cggcaacacg ctggccgaag ttgcaaatcg actttatgac
    27241 cattcatgcc agcaaagggc aacaggcgga ttacgtcatc atcgttggct tgcaggaggg
    27301 gagtgatggt tttccggctg cggcgcggga gtcgattatg gaagaggcgc tactgccacc
    27361 ggttgaggat ttcccggacg ctgaagaacg gcgtttaatg tacgtggcgc tgacccgggc
    27421 acgccatcgg gtatgggcac tgtttaacaa agagaatccc tctccctttg tggaaatact
    27481 gaaaaatctg gatgtgccgg tggcgagaaa accgtaagaa acaggtggcg tttgccacct
    27541 gtgcaatatt acttcagacg gtcagcgaga taacgctgat aatcggggat aagaatatcg
    27601 accgcgtcgt tgaaatgcgg cgactggata atgaagtctg ccgttgccac gttggtggcg
    27661 accggaatgt tccataccgt cgccagacgc agcaaggctt tcacgtcagg atcgtgcggc
    27721 acggcattta gtggatccca gaagaaaatc aatacatcaa ttttcccttc tgagatcaat
    27781 gcgccaacct gctggtcacc ccccattggg ccactcaaca tcgcgttgac gttcatgccg
    27841 gtcgcgcggg aaatcaagtt accggtagtg cctgttgcat acagtacgtg ttgttccagt
    27901 aacggttgat gccgttccac ccagctcatc agcatttgtt tgcagtgatc gtgtgccacc
    27961 agcgcaatat gtttccgcgc aggtaaagtg cgagtcgtca gttccataat gtacatccgt
    28021 agttaacttt cctacagatt actgtaagca cttatcgctg caagataaag accgaaaaag
    28081 cctgcgcaca ggcacaaaaa tctcaggaag atggttgttt ttccgcccat tgcaggaaag
    28141 tatttcgcgt ttgtgggtca gccagtttaa accaatactt cagccgttgt tctgtgagca
    28201 cctgagactg cggtggaata gcatccagct cggaaacacc cgaaagcagc gtactgtcat
    28261 ctgccatcat cgtggcaaat tgcggtagtg aagcgcgttt cgcggatttg ttatagcgtt
    28321 caacctcgac ctcataatca atcgtttttg ccgttgaggt aatggcgaga atatccagct
    28381 ttaccggaat cggcgtcgca tcgccatcca acaattcaag gcgcggcgcg gcatcaaaat
    28441 ggttagcttc ccgctcattc tccaggcgag gcaggcgaaa attgacctgg ttgatgagct
    28501 gggtattaaa actgaccacc agcggtgggg agatatacag ccgttcttca ctattggaaa
    28561 gatgaatcgt cttctcaacg cgaaacacta actgatgcgg tccattgtcc agttcaatgc
    28621 tgtccgcacc gcgcagcaga gagctggaaa cctttttgcc gtcgagtacc agaagatcga
    28681 catcggttga aagccgcagc gtggtggcaa aaacggatac cggcaaacat aatgcaatca
    28741 aggtggtcac gatgccggtt ttcataggta gctcctgtca aaagaccgcc cgaataataa
    28801 tgtgtcaaaa tttttcttta atcatactta ctaacatata gacatatttc ccgcttttgc
    28861 tctcattcat tcgtattagc tgcatggttg gcatgtcggc tttgtcgtac actttgttaa
    28921 acagtctgga ggaaataatg aaagaaaccg atattgcagg cattttaacg tccactcaca
    28981 ccattgcgct ggtgggggca agcgacaaac ccgatcgccc cagctatcgc gtgatgaaat
    29041 atctactcga ccaggggtat cacgtcattc cggtttcgcc aaaagttgcc ggcaaaacgc
    29101 tgctgggcca gcaggggtat ggcacgctgg cagacgttcc ggaaaaggtc gatatggtcg
    29161 atgttttccg taattcagaa gcggcgtggg gcgtggcaca ggaagctatt gccattggcg
    29221 cgaaaaccct ctggatgcaa ctgggggtga ttaatgagca agcggcagta ctggcacggg
    29281 atgccgggtt aaatgtggtg atggatcgct gcccggctat tgagatccct cgcctgggtc
    29341 tggccaaata aaaaatcccc ggaaggcaaa aaccttccgg ggatttgttc agggattagt
    29401 tacgcagacg cggggcctgg agttgtttgc ggatggtctg cgccagctca tccatggatg
    29461 gctgttcagg atgttcatcc tgcaattcgc tacttagctg ggcttcagcc aggtaggtat
    29521 gaaccggtag gccgttatcg tcctccatca ccacgtgata ccacggagcg gcgcgaagct
    29581 cgtcattcac cgccagctca tcaggcgacg gttcagagag cgaataaacc gggtcgatat
    29641 ccacgaccac tccgagataa cctaacaggg aatggcggac ctgctggccg ataccgaatt
    29701 tgctggcaat catagtcacc tcccgggaaa tctttacctt tgatataagg gtaaaattcc
    29761 acatttcaag ttacatgacg cgacaggcaa accctttcag atatagccct tccgggtagg
    29821 tagcgatcac cggatgatcg gctgcctgac ggaactgctc tataaattgt acatcacgac
    29881 cggcatcaat tgcggcatcc gcgatgattt tctgaaataa atcgctggtc atcagaccgg
    29941 aacaggagaa agtcaggaga ataccgcctt cattcagcaa ctgaatcgcc agcatgttga
    30001 tgtctttata accacgacac gcgcccatca actggctttt attctcaaca aacttcggcg
    30061 ggtccatcac gataacgtca aatttttcac cgcgatcgcg ataagtacgc agcaatttaa
    30121 agacatcatc acggacaaac tcagccttgc tcagatccag tttgttcagc tcaacgttct
    30181 gccgtgcaat atccagcgct tcctgggagg tatcaacgct gacaacctgg ctgcaaccgc
    30241 ccatcagtgc cgatacggcg aaaccaccgg tataggagaa acagttcagc acacgtttat
    30301 tttcaacgta gcggcgggta gccaggcggc tgtctcgctg gtccagatag tagcccgttt
    30361 tgtgtccgtg ctgaatatcc accagcagct tcatgccgtg ttcttcaatc ggcagcaggg
    30421 caggcggcaa ctcgccggtg acgaggccct gggtcagctc catcccttct tttttacgta
    30481 ccgcaacatc gctgcgatcg taaatcgcac attccgggta cagcgtttgc agggcactaa
    30541 ttaatgccgc gcgctgatat tctgcgccag cactcagcag ttgcagcacc agaaaattac
    30601 cgaaacgatc gatagtaata cccggcaggc catcagattc tccggcgatt aaacgatagc
    30661 tgtcgaggcc atctttttgc gccagccagt cacgccattt ttgtgcttgt tgcaaacggc
    30721 gggaaaaaaa agcaatgtcg atagactcag acgggtcaaa cgtccagacg cgcgcccgga
    30781 tttgcgaagc tggcgaataa gcgccgcgtg ctaaccattt tccctgatga tcaacaatat
    30841 cgatggtttc accgaggctg gctttacctt ccatgcgggc aacggccccg gaaaagaccc
    30901 acggatggcg acgaagtaat gatttttcgc gccctttggc taacactaaa cgtacactca
    30961 taatttactt ttctgtcgat gcaaagaaat gggcgtcatt gtccggagtt ttccatggat
    31021 ttgcaacgcg tccattaagg ataaggagag cagcaatgtc gaaagtctgc ataattgcct
    31081 ggatttacgg gcgagttcag ggcgtaggat ttcgctacac cacacagtac gaagcgaaaa
    31141 gactgggctt aaccgggtac gccaaaaatc ttgacgatgg cagcgtggaa gtggttgcct
    31201 gcggtgaaga agggcaggta gaaaaattaa tgcagtggtt aaagagtggc ggcccgcgtt
    31261 ctgcgcgtgt agaacgggtg ctcagcgagc cgcatcatcc ctcgggggaa ttaaccgatt
    31321 ttcgtatccg ttaaatacat tttaccggtt ttggcaggcc agcaattttg gtggcttgct
    31381 tagccgggcc tttcgggaac agtcggtata aatagcggct gttacctttt tcttcaccaa
    31441 atttattcgc catcgctttt accagcatac gaatcgccgg agacgtatta aattccaggt
    31501 agaagtcacg tacaaaacgc accacttccc agtgttctgg cgacagcgaa atcccttcgt
    31561 tctctgcaat caccactgcc agcggttcac tccactggct gctttctttg agatagcctt
    31621 cggtatccgt ttctatctct ttaccttcga agatcagcat aattattact accttaatca
    31681 gactggcggc agtgtaacaa aaaacaaagc cccgcataaa gcgaggctat gaaagtgtta
    31741 gcgggtgaga ttaatcgcgg ctggcgaagc ccagaatgct cagcaggctg acgaagatgt
    31801 tgtacagcga aacatacagg ctaaccgtgg cacgaatata gttcgtttca ccgccatgaa
    31861 tgatgttgct ggtttcaaac aagatagcgc cagaggagat cagaatgaag accgcgctga
    31921 tcgccagatg cagagcaggc agttgcagga agatattagc aaccatacca atcagtacca
    31981 ccacaatacc cgccatcagc ataccgccga ggaacgacat atctttgcgg gtggtcagca
    32041 catatgcaga gcagcagaag aacactaacg ccgttccgcc cagtgccata gcgattacgt
    32101 cacccattcc ggcagacaga taggtgttca gaattggtcc gaggatataa cccagaaaac
    32161 cggtaaaggc gaatgcggag ataatcccgg tcggcttatt cgccgtttta taggtcagga
    32221 acatcaaacc atacataccc accagcgtca gaatcagacc cggagatggc agcatcagca
    32281 ccgtactggc agttgcggta atcgccgaaa aggccagcgt caggctcagc agaaaatagg
    32341 tattacgcag caccttatgg gtgctaagca gtgatgtacg gtcatgtgaa gaactaacaa
    32401 tacgatccat tgagtcactc tctatgacag atgtaattaa ttaagcagca taatgataat
    32461 gcgtaagggc acccagaagt tttacccatc tttacgcatt tgatctggaa caggtttaac
    32521 agcggattat caggtcatta agcaaatata acgccctgag aattccgaca ggcaaaagaa
    32581 aaaggggtta gcatttagct aaccccttat cttatttggc ggaagcgcag agattcgaac
    32641 tctggaaccc tttcgggtcg ccggttttca agaccggtgc cttcaaccgc tcggccacac
    32701 ttccggaatg acgcgcacta taaacatccc gatgcggcgt gtaaacccct aatttgtttg
    32761 tttgcctgaa aaacagccaa aagtgcattg atagcgtgaa ataacagcag attgatcatt
    32821 tcatcaccat gaattccttc tcttttactc gtttagcaac cggctaaaca tccccatcgc
    32881 ccggctaaag aaaaataggt ccatttttat cgctaaaaga taaatccaca cagtttgtgt
    32941 tgttttgtgc aaaagtttca ctacgcttta ttaacaatac tttctggcga cgtgtgccag
    33001 tgcagaagga tgagctttcg ttttcagcat ctcacgtgaa gcgatggttt gccttgctac
    33061 agggacgtcg cttgccgacc ataagcgccc ggtgtcctgc cggtgttgct aggagaagag
    33121 acgtgcgata tgaataacga ggaaacattt taccaggcca tgcggcgtca gggcgttacc
    33181 cggcgcagct ttctcaaata ttgtagtctg gctgccacgt cgctgggatt aggcgcggga
    33241 atggcaccaa agattgcctg ggcgctggag aacaaaccgc gcattccggt ggtatggatc
    33301 cacggtctgg aatgtacctg ctgtaccgaa tcttttatcc gctccgctca cccactgacg
    33361 aaggacgtca tcctttccct gatttccctc tattacgacg ataccctgat ggctgccgcc
    33421 ggaacccagg cggaagaagt ctttgaagac atcatcacgc aatataatgg caaatatatc
    33481 ctcgcagtag aaggtaatcc gccgctgggc gagcagggga tgttctgtat cagcagcggt
    33541 cgaccgttta ttgagaaact caaacgtgcc gctgccggag ccagcgcgat tatcgcctgg
    33601 ggaacctgcg cgtcctgggg ctgcgtgcag gccgcgcgac ctaatccgac acaggcaacg
    33661 cctatcgaca aagtcatcac cgacaaaccc attatcaaag tacctagctg cccgccgatc
    33721 ccggatgtga tgagcgccat cattacttac atggtgacct ttgatcgctt gccagatgtc
    33781 gacagaatgg gtcgtccact gatgttttat ggtcagcgta tccacgataa atgctatcgc
    33841 cgcgctcact tcgacgccgg agagttcgtc cagagttggg atgatgacgc tgcccgcaaa
    33901 ggttattgcc tgtacaaaat gggctgcaaa gggcctacca cctataacgc ctgttcctcc
    33961 acacgctgga atgatggcgt ttctttccca atccagtctg gtcacggctg cctgggctgt
    34021 gcggaaaatg gtttctggga tcgcggttcg ttctacagcc gcgtggtcga tattccgcaa
    34081 atgggtactc attccaccgc cgataccgtc ggtttaaccg cgcttggcgt ggtggcagca
    34141 gctgttggtg tgcacgcagt tgccagcgcc gttgaccaac gcagacgtca taaccagcaa
    34201 cctacagaaa ccgaacatca gccaggcaat gaggataaac aggcatgagc actcagtacg
    34261 aaactcaggg atacaccatc aataatgccg gacgccgcct ggtggtcgac ccgattacgc
    34321 gcatcgaagg ccacatgcgc tgcgaagtga atattaacga tcagaatgtg atcaccaatg
    34381 ccgtctcctg cggcaccatg tttcgcgggc tggagatcat cctgcaaggg cgcgacccgc
    34441 gcgatgcgtg ggcgttcgtt gaacgtatct gcggcgtctg tactggcgta cacgccctgg
    34501 cttcggttta cgccatcgaa gatgccatcg gtattaaagt gccggacaac gccaatatca
    34561 tccgcaacat tatgctgaca acgctctggt gccacgatca tctggtgcac ttctatcagc
    34621 ttgccgggat ggactggatc gacgtgttag atgcgctgaa agccgacccg cgaaaaacct
    34681 ccgaactggc gcaaagtctc tcctcttggc cgaaatcatc ccctagctat ttcttcgacg
    34741 tacaaaatcg cctgaagaaa ttcgttgaag gcgggcagtt ggggatcttc cgcaatggct
    34801 actgggggca cccgcagtac aaattgccgc tagaagccaa cctgatgggc tttgcccact
    34861 atctcgaagc tctcgatttc caacgtgaaa ttgtcaaaat tcacgcggtc tttggcggta
    34921 aaaacccgca tccaaactgg attgtcggcg ggatgccttg cgccattaac attgacgaaa
    34981 gcggcgcggt cggggcggtc aatatggaac gcctgaacct ggtgcagtcg attatcaccc
    35041 gcacggcaga cttcattaac aacgtgatga tccccgacgc cttagccatc ggtcagttca
    35101 acaaaccgtg gagcgaaatc ggcacgggtc tttctgataa atgtgtcctc agctacggcg
    35161 cattcccgga tattgccaac gactttggtg agaaaagtct gctgatgcct ggcggcgcgg
    35221 tgattaacgg cgacttcaac aatgtgctgc cagtggattt ggttgatccg cagcaggtgc
    35281 aggagtttgt cgatcacgcc tggtatcgtt atcccaacga tcaggtcggg cgtcatccat
    35341 tcgatggcat taccgacccg tggtacaacc ccggcgatgt caaaggcagc gataccaaca
    35401 ttcagcagct gaatgaacag gaacgctact cgtggatcaa agcgccacgc tggcgcggta
    35461 acgcgatgga agtggggccg ctggcacgca cgttaatcgc ttatcacaaa ggcgatgctg
    35521 cgaccgttga gtcggtcgat cgcatgatgt cggcgttgaa cctgccgctt tccggtatcc
    35581 agtcaacgtt gggccgcagg taagttgcag tatttcttcg acaagctgat gaccaacctg
    35641 aaaaatggca atctcgccac tgcttccact gaaaaatggg aacctgcaac ctggccgaca
    35701 gagtgccgtg gcgtcggctt taccgaagcg ccgcgcggag cgttaggcca ctgggccgcc
    35761 attcgcgatg gcaagattga tctctaccag tgcgtggtgc cgaccacctg gaacgccagc
    35821 ccgcgcgatc ccaaagggca gattggcgct tatgaagcgg cgctgatgaa caccaaaatg
    35881 gcgatccccg agcaaccgct ggagatcctg cgtactctgc acagctttga tccgtgcctc
    35941 gcctgttcaa ctcatgtgct gggcgacgac ggtagcgagc tgatctccgt gcaggtgcgt
    36001 taacagcgaa ggagaatcat catgcaacag aaaagcgaca acattgtcag ccactatgtc
    36061 tttgaagcac cagttcgtat ctggcactgg ctgacggtgt tatgcatggc ggtgttgatg
    36121 gtcaccggtt actttatcgg caagccgcta ccttccgtca gtggcgaggc gacgtatctg
    36181 ttctatatgg gttacatcag gttaattcac ttcagcgccg ggatgatttt taccgtggtc
    36241 ttgctgatgc ggatctactg ggcttttgtt ggcaatcgtt actcccgcga gctgtttatc
    36301 gtgccggtat ggcgtaaaag ctggtggcag ggcgtgtggt atgaaatccg ctggtatctg
    36361 tttctggcaa aacgtccgag tgccgatata ggccataacc ccatcgccca ggcggcgatg
    36421 ttcggctatt tcctgatgtc ggtctttatg atcatcaccg gttttgcgct gtacagcgaa
    36481 cacagccagt acgctatttt tgcgccgttc cgttatgtgg tggaattttt ctactggacg
    36541 ggtggcaact caatggacat tcacagctgg catcggctgg ggatgtggct gattggggcg
    36601 tttgtgatcg gtcatgtcta catggcgctg cgtgaagaca tcatgtccga cgacacggtg
    36661 atctccacca tggtcaacgg ctaccgtagc cacaaatttg gcaaaataag taacaaggag
    36721 cgttcatgag cgagcaacgc gtggtggtca tggggctggg caacctgctg tgggccgatg
    36781 aaggcttcgg cgtgcgggtg gcggaacggc tgtatgccca ttaccactgg cccgagtatg
    36841 tggagattgt cgatggcggt actcagggac tgaacttgct ggggtatgtc gaaagcgcca
    36901 gccatctgtt gattctcgat gccattgact acgggctgga acctggaagg ctgcgaacct
    36961 atgccggaga acgcattccg gcctatctca gcgcgaagaa aatgagcctg catcagaaca
    37021 gtttctccga agtgctggcg ctggcagata tccgcggaca tctgccagca catattgccc
    37081 tcgtcgggct gcaacccgcg atgctcgacg actacggcgg cagcctgagc gaactggcac
    37141 gggagcaact gcccgctgcg gaacaatcgg cgctggcgca gttggctgcg tggggaagtg
    37201 tgccgcaacc ggctaatgaa tcgcgttgtc tcaattatga ctgtctgtcg atggaaaatt
    37261 acgaaggcgt tcgcttgcgc cagtaccgga tgacacagga ggagcaggga tgagcaacga
    37321 cacgccattt gatgcgttgt ggcaacgaat gctggcgcgc ggctggacgc cggtcagtga
    37381 atcccgtctt gacgactggc ttacgcaagc gccagacggc gtggtgttat taagcagtga
    37441 cccgaaacgc acgccagagg tcagcgataa tccggtaatg attggcgaat tactgcacga
    37501 gtttcccgac tatacatggc aggtggcgat tgctgacctt gagcagagcg aagccatcgg
    37561 cgatcgcttt ggcgcctttc gctttcctgc cactttagtg tttaccggcg gaaactatcg
    37621 cggcgtgctg aatggtattc acccgtgggc ggaactgata aacctgatgc gcgggcttgt
    37681 cgaaccgcag caggagcgtg cctcatgagc gaaacttttt tccatctgct ggggccagga
    37741 acgcaaccga acgatgacag tttcagcatg aatccactgc cgatcacctg tcaggtgaat
    37801 gatgaaccga gtatggcggc cttggagcaa tgtgctcaca gcccgcaggt gattgcgctg
    37861 ttaaacgagt tacaacatca actaagcgaa cgccagccgc cgttgggcga ggtgctggca
    37921 gtcgatctgt taaatctcaa cgccgacgat cgtcacttta tcaatacgct tctcggggaa
    37981 ggggaagtgt cagtgcgcat tcagcaggct gacgacagta aaagtgaaat acaggaggcg
    38041 atcttctgcg gattatggcg ggtgcgcaga cgtcgcggcg aaaagttgct ggaggacaaa
    38101 ctggaggctg gctgcgcgcc gctggcgttg tggcaggcgg caacgcaaaa tctcttgccg
    38161 acagattcgc tgttaccgcc gcccattgat ggcctgatga atggcctacc gttggcgcat
    38221 gagttactgg cacatgtacg taaccccgac gcgcagccgc acagcattaa tctgacgcaa
    38281 ttacccatca gcgaggctga tcggcttttt ctctcacgtc tctgtgggcc gggaaatatt
    38341 cagattcgta ccattggcta tggcgagagc tatatcaact ccacggggtt acgccatgtc
    38401 tggcatttac gctgtacgga caccttaaaa ggcccgttac tggaaagtta tgaaatctgc
    38461 ccaataccgg aagtggtgct ggcagcgcca gaagatttgg tcgactctgc gcagcggctt
    38521 agcgaggtat gtcagtggct ggcggaagct gcaccgacgt aaaaagacgg taagtatcgc
    38581 tttcagtctt atgaatatcg caatcggcga atacctctgg tcgtagagtt tcaggataaa
    38641 gagggagatc taacattatc gggttatttt tctctcttcg cctacaggag tgcgcatgtg
    38701 ggatgtcatt gatttatcgc gctggcagtt tgctctgacc gcgctgtatc actttttatt
    38761 tgtacccctt accctggggc tgattttttt gctggctatc atggaaacca tttacgtggt
    38821 caccggcaaa acaatctacc gcgatatgac gcgcttctgg ggtaagctct tcggtatcaa
    38881 ttttgctctt ggcgtggcta ccggcctgac catggagttt cagtttggta ctaactggtc
    38941 attctattcc aactatgtgg gcgatatttt tggcgcacca ctggcgatgg aagcattaat
    39001 ggccttcttc ctcgaatcca cctttgtcgg gctgttcttc ttcggctggc aacggctgaa
    39061 taaataccag cacctgctgg tgacgtggct ggtggcgttc ggttcaaatc tctctgcatt
    39121 gtggatattg aatgccaacg gttggatgca atacccgacc ggtgcgcatt ttgatatcga
    39181 caccctgcgt atggagatga ccagcttcag cgagctggtc tttaacccgg tcagccaggt
    39241 gaaatttgtg cacaccgtca tggcgggcta cgtgaccggg gccatgttta ttatggcgat
    39301 cagcgcctgg tatttactgc gcggacggga gcgcgatgtc gcattacgct cgtttgccat
    39361 cggttccgtc ttcggtactc tggcgattat cggtaccctg caactcggag acagttctgc
    39421 gtatgaagtc gcgcaagtac aaccggtaaa actggcggcg atggaagggg agtggcaaac
    39481 ggaacctgct cctgcaccgt tccatgtggt tgcctggccg gaacaggatc aagagcgtaa
    39541 cgcctttgcc atcaaaattc ccgcgctgct agggatcctc gccactcact cattagataa
    39601 acccgtgccg ggtctgaaga atttgatggc tgaaacctac ccacgcttgc aacgcggacg
    39661 tatggcctgg ctgttaatgc aggaaatatc tcaaggcaat cgtgagccgc atgtgttgca
    39721 ggcattccgg gagctggaag gcgaccttgg ttacggcatg ttgctctccc gctatgcgcc
    39781 ggatatgaat catgtcacag ccgcacagta ccaggcggcg atgcgtggcg cgatacctca
    39841 ggttgcgccg gtattctgga gtttccgcat catggtgggc tgtggttccc tgctgctact
    39901 ggtgatgctg attgcgcttg tccagacgct gcgtggcaaa atcgaccagc atcgctgggt
    39961 gctgaaaatg gcgctctgga gtttgccgtt gccgtggatt gcgattgaag ccgggtggtt
    40021 tatgaccgag tttggtcgtc agccgtgggc gatacaggac atcttaccga catactccgc
    40081 gcactccgct ttaaccacag gacaactggc tttctcactg atcatgatcg taggtcttta
    40141 caccctgttc ttaatcgccg aagtctacct gatgcagaaa tatgcccgtc tggggccgag
    40201 cgcgatgcag agtgaacaac cgacgcagca acaggggtaa aggagaaaat catgtttgat
    40261 tatgaaacat tgcgcttcat ctggtggctg ctgattggcg tgatcctggt ggtctttatg
    40321 atctccgacg gatttgacat ggggatcggc tgtttgctgc cgctggtggc gcgtaatgat
    40381 gatgaacgcc ggatagtgat taatagcgtt ggtgcgcact gggaaggcaa tcaggtctgg
    40441 ttgatcctcg caggtggggc attatttgcc gcctggccca gagtgtatgc agcggcgttt
    40501 tccggctttt atgtggcgat gatcctggtg ctgtgctcgc tgttcttccg tccgctggcc
    40561 tttgattatc gcggaaaaat tgccgatgca cgctggcgta aaatgtggga tgccggtctg
    40621 gtcatcggca gtctggtgcc gccggtagtc ttcggtatcg ccttcggcaa cttgttgctc
    40681 ggcgtgccgt ttgccttcac accgcaatta cgcgtggagt atctcggcag cttctggcaa
    40741 ctgctgacgc cattcccttt attgtgtggt ttgctcagcc ttgggatggt gattttgcaa
    40801 ggtggcgtct ggttacaact gaaaactgtt ggtgtgattc atctgcgttc acagctggcg
    40861 actaaacgcg ctgcactgtt ggtgatgctg tgctttttgc tggcaggtta ctggctgtgg
    40921 gtcggtattg atggctttgt actgctcgcc caggatgcta acggtccttc caatccgtta
    40981 atgaaactgg tggcagtgct acctggtgcc tggatgaata atttcgtcga atcgcccgtt
    41041 ttgtggatct tcccgctgct ggggttcttc tgcccattgc tgacggtgat ggcgatttat
    41101 cgtggtcgcc cgggttgggg atttttgatg gcatcattga tgcaatttgg cgtgattttc
    41161 acggcaggca tcacgctgtt cccctttgtc atgccgtcaa gtgtgagtcc aatctccagc
    41221 ctgacgttgt gggacagcac ttccagtcag ctgacgctga gcattatgtt ggtaatcgtg
    41281 ctgatatttt tgcccattgt gttgctctac actctctgga gctactacaa aatgtgggga
    41341 cgcatgacaa cagaaactct ccgccgtaac gaaaacgagt tgtactaagg agcaggaaca
    41401 tgtggtattt actttggttc gtcggcattt tgttgatgtg ttcgctctcc acccttgtgt
    41461 tggtatggct ggacctgcgt ctgaaaagtt aacgaacgta ggcctgatgc ggcgcattag
    41521 catcgcatca ggcaatcaat aatgttagat atgaaaagcg gaaacatatc gatgaaagcg
    41581 atcttaatcc catttttatc tcttctgatt ccgttaaccc cgcaatctgc attcgctcag
    41641 agtgagccgg agctgaagct ggaaagtatg gtgattgtca gtcgtcatgg tgtgcgtgct
    41701 ccaaccaagg ccacgcaact gatgcaggat gtcaccccag acgcatggcc aacttggccg
    41761 gtaaaactgg gttggctgac accgcgcggt ggtgagctaa tcgcctatct cggacattac
    41821 caacgccagc gtctggtagc cgacggattg ctggcgaaaa agggctgccc gcagtctgct
    41881 caggtcgcga ttattgctga tgtcgacgag cgtacccgta aaacaggcga agccttcgcc
    41941 gccgggctgg cacctgactg tgcaataacc gtacataccc aggcagatac gtccagtccc
    42001 gatccgttat ttaatcctct aaaaactggc gtttgccaac tggataacgc gaacgtgact
    42061 gacgcgatcc tttgcagggc aggagggtca attgctgact ttaccgggca tcggcaaacg
    42121 gcgtttcgcg aactggaacg ggtgcttaat ttcccgcaat caaacttgtg ccttaaccgt
    42181 gagaaacagg acgaaagctg ttcattaacg caggcattac catcggaact caaggtgagc
    42241 gccgacaatg tctcattaac cggtgcggta agcctcgcat caatgctgac ggagatattt
    42301 ctcctgcaac aggcacaagg aatgccggag ccggggtggg gaaggatcac cgattcacac
    42361 cagtggaaca ccttgctaag tttgcataac gcgcaatttt atttactaca acgcacgccg
    42421 gaggttgccc gcagtcgcgc caccccgtta ttggatttga tcatggcagc gttgacgccc
    42481 catccaccgc aaaaacaggc gtatggtgtg acattaccca cttcagtgct gtttattgcc
    42541 ggacacgata ctaatctggc aaatctcggc ggcgcactgg agctcaactg gacgcttcca
    42601 ggtcagccgg ataacacgcc gccaggtggt gaactggtgt ttgaacgctg gcgtcggcta
    42661 agcgataaca gccagtggat tcaggtttcg ctggtcttcc agactttaca gcagatgcgt
    42721 gataaaacgc cgctgtcatt aaatacgccg cccggagagg tgaaactgac cctggcagga
    42781 tgtgaagagc gaaatgcgca gggcatgtgt tcgttggcag gttttacgca aatcgtgaat
    42841 gaagcacgca taccggcgtg cagtttgtaa tgcataaaaa agagcattca gttacctgaa
    42901 tgctctgagg ctgatgacaa acgaagaact gtctaatgcg tagaccggaa aaggcgttca
    42961 cgccgcatcc ggtcactttc agttttactc tttctcggag taactataac cgtaatagtt
    43021 atagccgtaa ctgtaagcgg tgctggcgcg tttaatcaca ccattgagga ttgcgccttt
    43081 aatattaacg ccagcctgtt ccagacgctg cattgacaag ctcacctctt tggcggtgtt
    43141 caagccaaaa cgcgcaacca gcaggctggt gccgacagag cgtcccacga ccgcagcatc
    43201 gctcaccgcc agc
//
LOCUS       DAJVQH010000017        40717 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJVQH010000017 DAJVQH010000000
VERSION     DAJVQH010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.017"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(1..798)
                     /gene="glpF"
                     /locus_tag="OQM87_001071"
                     /pseudo
     CDS             complement(1..798)
                     /gene="glpF"
                     /locus_tag="OQM87_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            1224..1469
                     /gene="zapB"
                     /locus_tag="OQM87_001072"
     CDS             1224..1469
                     /gene="zapB"
                     /locus_tag="OQM87_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCS1507295.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            complement(1554..2039)
                     /gene="rraA"
                     /locus_tag="OQM87_001073"
     CDS             complement(1554..2039)
                     /gene="rraA"
                     /locus_tag="OQM87_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCS1507296.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(2132..3058)
                     /gene="menA"
                     /locus_tag="OQM87_001074"
     CDS             complement(2132..3058)
                     /gene="menA"
                     /locus_tag="OQM87_001074"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCS1507297.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            complement(3125..4456)
                     /gene="hslU"
                     /locus_tag="OQM87_001075"
     CDS             complement(3125..4456)
                     /gene="hslU"
                     /locus_tag="OQM87_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCS1507298.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            complement(4466..4996)
                     /gene="hslV"
                     /locus_tag="OQM87_001076"
     CDS             complement(4466..4996)
                     /gene="hslV"
                     /locus_tag="OQM87_001076"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCS1507299.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            complement(5089..6048)
                     /gene="ftsN"
                     /locus_tag="OQM87_001077"
     CDS             complement(5089..6048)
                     /gene="ftsN"
                     /locus_tag="OQM87_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCS1507300.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            complement(6140..7165)
                     /gene="cytR"
                     /locus_tag="OQM87_001078"
     CDS             complement(6140..7165)
                     /gene="cytR"
                     /locus_tag="OQM87_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCS1507301.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            complement(7321..9519)
                     /gene="priA"
                     /locus_tag="OQM87_001079"
     CDS             complement(7321..9519)
                     /gene="priA"
                     /locus_tag="OQM87_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCS1507302.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            9722..9934
                     /gene="rpmE"
                     /locus_tag="OQM87_001080"
     CDS             9722..9934
                     /gene="rpmE"
                     /locus_tag="OQM87_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCS1507303.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            complement(9995..10603)
                     /locus_tag="OQM87_001081"
     CDS             complement(9995..10603)
                     /locus_tag="OQM87_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCS1507304.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(10787..11104)
                     /gene="metJ"
                     /locus_tag="OQM87_001082"
     CDS             complement(10787..11104)
                     /gene="metJ"
                     /locus_tag="OQM87_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCS1507305.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            11381..12541
                     /gene="metB"
                     /locus_tag="OQM87_001083"
     CDS             11381..12541
                     /gene="metB"
                     /locus_tag="OQM87_001083"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCS1507306.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            12544..14976
                     /gene="metL"
                     /locus_tag="OQM87_001084"
     CDS             12544..14976
                     /gene="metL"
                     /locus_tag="OQM87_001084"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCS1507307.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            15325..16215
                     /gene="metF"
                     /locus_tag="OQM87_001085"
     CDS             15325..16215
                     /gene="metF"
                     /locus_tag="OQM87_001085"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCS1507308.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            16544..18724
                     /gene="katG"
                     /locus_tag="OQM87_001086"
     CDS             16544..18724
                     /gene="katG"
                     /locus_tag="OQM87_001086"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCS1507309.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALLVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            18818..19723
                     /gene="yijE"
                     /locus_tag="OQM87_001087"
     CDS             18818..19723
                     /gene="yijE"
                     /locus_tag="OQM87_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCS1507310.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(19750..20367)
                     /locus_tag="OQM87_001088"
     CDS             complement(19750..20367)
                     /locus_tag="OQM87_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCS1507311.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(20642..21734)
                     /gene="gldA"
                     /locus_tag="OQM87_001089"
                     /pseudo
     CDS             complement(20642..21734)
                     /gene="gldA"
                     /locus_tag="OQM87_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(21745..22407)
                     /gene="fsa"
                     /locus_tag="OQM87_001090"
     CDS             complement(21745..22407)
                     /gene="fsa"
                     /locus_tag="OQM87_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1507312.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPCQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(22419..24920)
                     /gene="ptsP"
                     /locus_tag="OQM87_001091"
     CDS             complement(22419..24920)
                     /gene="ptsP"
                     /locus_tag="OQM87_001091"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1507313.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRVRTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            25229..26308
                     /locus_tag="OQM87_001092"
     CDS             25229..26308
                     /locus_tag="OQM87_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCS1507314.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            26323..26643
                     /locus_tag="OQM87_001093"
     CDS             26323..26643
                     /locus_tag="OQM87_001093"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1507315.1"
                     /translation="MTKIIAVTACPSGVAHTYMVAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            26694..28991
                     /locus_tag="OQM87_001094"
     CDS             26694..28991
                     /locus_tag="OQM87_001094"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1507316.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            28957..29835
                     /locus_tag="OQM87_001095"
     CDS             28957..29835
                     /locus_tag="OQM87_001095"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCS1507317.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            29837..30178
                     /locus_tag="OQM87_001096"
     CDS             29837..30178
                     /locus_tag="OQM87_001096"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1507318.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(30165..31016)
                     /locus_tag="OQM87_001097"
     CDS             complement(30165..31016)
                     /locus_tag="OQM87_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1507319.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(31165..32898)
                     /locus_tag="OQM87_001098"
     CDS             complement(31165..32898)
                     /locus_tag="OQM87_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCS1507320.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(33081..35732)
                     /gene="ppc"
                     /locus_tag="OQM87_001099"
     CDS             complement(33081..35732)
                     /gene="ppc"
                     /locus_tag="OQM87_001099"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCS1507321.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLDYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(36084..37235)
                     /gene="argE"
                     /locus_tag="OQM87_001100"
     CDS             complement(36084..37235)
                     /gene="argE"
                     /locus_tag="OQM87_001100"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCS1507322.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            37389..38393
                     /gene="argC"
                     /locus_tag="OQM87_001101"
     CDS             37389..38393
                     /gene="argC"
                     /locus_tag="OQM87_001101"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCS1507323.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            38401..39177
                     /gene="argB"
                     /locus_tag="OQM87_001102"
     CDS             38401..39177
                     /gene="argB"
                     /locus_tag="OQM87_001102"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCS1507324.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            39238..40611
                     /gene="argH"
                     /locus_tag="OQM87_001103"
     CDS             39238..40611
                     /gene="argH"
                     /locus_tag="OQM87_001103"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCS1507325.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         9282 a        10459 c        11179 g         9797 t
ORIGIN      
        1 cacaacacag atatcgcaag gcaaatggcg accaatcagt ttgcggtagg caaatgcacc
       61 tacaatcgcg ccaacgatag ggccgaaaag cggcaccagg aagtaaggaa tgtctctgcc
      121 gccggtaaag gcgacattgc cccagcccgc cagctaggca aagactttcg gaccgaagtc
      181 acgcgctggg ttcatggcaa aacctgtcaa tgggcccata gatgcgccaa tgaccgcaat
      241 cagtagacca atcagcaagg gagccaaagg gccgcgtggt acaccgttgc catcgtccgt
      301 taacgccagg ataagcccca tcagaatagc ggtaatcacc atctcaactg cgaaagcctg
      361 cacaaaattg atatgaggat tagggtaagt agagaaagtg ccagccagat caacactttc
      421 aacgctgccg cgaacaatgt gatgagtctg ctcgaagtcg aaaaataaat tgtagtaaag
      481 cccgtaaact aaagccgcag cgcagaaagc gccggcaact tgtgaaacga taaaaggaat
      541 aactttgcgc ttgtcgaaac aggcaaacag ccacaatgca atggtaacag cgggattaag
      601 atgcgcgccg gaaacccctg cggtcaggta gatggccatt gccaccccca gtccccaaat
      661 gacactgatt tcccactgac caaaagacgc accagcgact tttagtgctg caacgcaacc
      721 cacaccgaaa aaaatcaaca acccggtacc gaggaattca gcaatgcact ggcctttcaa
      781 ggttgatgtt tgactcataa tcggatcctg aagagttaat gtttgttgta tgtgtgaaag
      841 tcacggacct ccacgatgct tgtaggcatg ctgttaactt atcgttaacg agcaaaaacg
      901 agaaatatcg aacttaaaat gtgtgtgctt cgtcataaaa tgagcgttat cgcgccattt
      961 attccatctg attgtacatg atgcatgttt gttagatcat tcgcgcaaca aatttattaa
     1021 cattttcgat tgatgtggat tatgtcacta ggagaaggat gaaaagtgtg gcaaaccgta
     1081 atctgcaagg cgtgccgctg gacagcgatg gcgcggcttc atacaatcgg agctaactaa
     1141 agtgcgctcg tatttattaa ggcgtcaccg gtaatcggga cgaggatttt ttatccatca
     1201 acgccttgca attcaggaga ggtatgacaa tgtcattaga agtgtttgag aaactggaag
     1261 caaaagtaca gcaggcgatt gataccatca ctctgttgca gatggaaatc gaagagctga
     1321 aagaaaaaaa caactcactg tcgcaggaag ttcaaaatgc ccagcatcag cgcgaagagc
     1381 tggagcgtga gaacaaccat ctgaaagaac agcagaacgg ctggcaggaa cgtctgcagg
     1441 ccctgctggg tcgcatggaa gaggtctgat tacgcttcat ctttcgcgta atttctttac
     1501 tgtgtaggcc aacaggtaac gcagaaaaaa ggcaccttgc ggtgcctttc ttatcattca
     1561 atatccagcg gatcttctga aagaataatc ccggtattgt cggcataaag atggtcgccg
     1621 gagaagaagg tgacaccgcc aaaattgacg cggacatcgc tttcgccaat gccttcgcca
     1681 gcggcaccaa ccggaattgc cgccatcgcc tggatgccga tatccaactc ttccaggtca
     1741 tctacctgac gcaccgcgcc gtaaatgacc agaccttccc attcattttg tactgccaga
     1801 cgcgccagtt cagcatcgac cagtgcgcga cgaacagaac caccgccatc gacgacaaga
     1861 acacgaccac ggccattctg ttcgagcaga tcgtacagca acccgttgtc ctcgaaacat
     1921 tttaccgtga ttatttgtcc gccaaacgac gcccgtccgc caaagttgga gaacagcggt
     1981 tccacgacgt taacatcttc ttgatagatg tcacaaagct cggaagtatc gtatttcata
     2041 ggcttaacat tcagttgctg cgagaatttt cagtatatcg cgctatgtgg gctgttggca
     2101 aagtcatcaa ttgttaattg atatttgtca gttatgctgc ccactggctt aggaatatcc
     2161 ctaaaacaaa cagcaggtta gtcagtaacg ctcccttgac agtacgttcc agcattggtc
     2221 gcatcgccac cgggtccatt tcccgcatca cataacgggc ttgcttcacc agtaatggtg
     2281 ccgccagcag gaacagccag ccccacaggc tatgcagcga aaagagatta aacagcgcca
     2341 gacacaccag cgagcccatc agcaggcagg catgataacg acgcgcgttc acttcaccta
     2401 agcgcaccac cagcgtgttt ttgccatttt cgcggtcgct attgatatca cgcaggttat
     2461 taatattcag tactgccgtt gccagcaggc cgcatgcggt cgccggaagg atcagtgccg
     2521 gaatcaatgt atgagcctgt aaataccagc tccccatgac actcaaccag ccaaagaaaa
     2581 ccagtacgga aatatcaccc agaccgatat aaccataagg acgattgccc acggtgtagg
     2641 tgatagcggc aatgatcgac aacccgccaa gaatcaggaa accgacaaaa tcggccagcg
     2701 tatggcatgc cactgcaacc agtgccagcc cggagagaca gatgagcacg acggtaataa
     2761 tgagcgcccg tttcatcttt tgctgggtaa tgaccccttt ttgcatgccg cgtagcggcc
     2821 caatgcggtc aggtttatcg ctgcctttta ccgcatcgcc gtaatcattg gcgaggttag
     2881 aaaggatctg taatagcccg gcggtaatta gtgccagcag ggcgaccagc ggatcgaagt
     2941 gaccttgcca ccatgccagc gccgtcccga cgataattgc agcaaaggcg agggggaggg
     3001 ttttaggtcg taaactttcc agccacgcct gagttcggct aatttgttgt tcagtcataa
     3061 tacgcgccaa taaaaatggg gcctttcagc cccatcaaac aatgatgaaa atgattgaac
     3121 gcgattatag gataaaacgg ctcagatctt catctgccac caacgcatcc agatgtttgc
     3181 tcacataatc tgcgtcaata gtgatatttt gaccgcttaa atcgctggcg tcgtaggaaa
     3241 tctcttccat taaacgctcc agaacagtgt gtaaacgacg agcaccgatg ttttcggtag
     3301 attcgttcac ctgccatgcc gcttccgcga tgcgtttaat accggagtcg gtaaactcga
     3361 tatttacgcc ttcagtcgcc atcagtgctt tgtactgcac ggtgatagag gcattcggct
     3421 cggtcagaat acgctcgaag tcgctggtgg tcagtgcctg cagttcaacg cggattggca
     3481 gccgaccttg cagttccggg atcaggtcag acggtttcgc aatctggaac gcgccagaag
     3541 cgataaacag aatgtggtca gttttgacca tcccgtgttt ggtggaaacg gtgcaacctt
     3601 ctaccagcgg cagcaggtca cgctgaacgc cttcacgaga aacatccgga ccggaagact
     3661 cgccgcgctt acagattttg tcgatttcgt cgataaacac gatcccgtgc tgctcaacag
     3721 cgtcgatagc gtcttgcttc agctcttccg ggttcaccag tttcgccgct tcttcttcaa
     3781 tcagcagctt catggcgtct ttgattttca gcttacgcgc tttttgcttc tggccgccca
     3841 ggttctggaa catggactgc agctggctgg tcatctcttc catgcccgga ggagccataa
     3901 tttcaacgcc catcggtgct gcggcaagat cgatctcgat ttctttgtca tcaagctggc
     3961 cttcacgcag ttttttgcgg aatgcctgac gagcagcgga aggttcctgc tgctgttcag
     4021 tctgtcccca gttgttttta gcaggtggga tcagcacgtc gagaatacgt tcttctgcca
     4081 gttcttcagc gcgataacgg tttttctcga tagcctggac gcgtaccatt ttcacggcgg
     4141 catcggtcag atcgcgaata atagaatcaa cttccttacc gacgtagccc acttcggtaa
     4201 atttggtcgc ttcaactttg atgaacggcg cattcgccag cttagccaga cgacgggcga
     4261 tttcagtttt accgacaccg gtcgggccga tcatcaggat atttttcggg gtcacttcat
     4321 ggcgcagctc ttcgttgagc tgcatgcgac gccagcggtt acgcagagca atcgccacag
     4381 aacgcttggc gttgtcctgg ccgatgatgt gcttatccag ttcgctgacg atttcgcgtg
     4441 gggtcatttc agacatggga gatccttacg ctttgtagct taattcttcg atggtgtgga
     4501 aatggttggt atagatgcaa atgtcgcctg caatatccaa cgccttttca gcaatttcac
     4561 gggcgctaag ttcagtgttt tctaacagcg cgcgcgccgc agcctgggcg taagggccgc
     4621 cggagccgat agcaataaga tcgttttctg gctgcaccac gtcaccgtta ccggtgatga
     4681 taagcgatgc agtttcatcc gcgactgcca gcagtgcttc aagtttgcgc agcatgcgat
     4741 cggtacgcca gtcttttgcc agctcaacgg cggctttaac cagatggccc tgatgcattt
     4801 ccagtttacg ttcaaacagt tcgaacagcg taaaagcatc cgcagtaccg cccgcaaagc
     4861 ccgcgatgac tttgtcgttg tacagacggc ggaccttttt cacgttgcct ttcattacgg
     4921 tattgcccaa cgtggcctga ccatcaccag cgatgaccac atggccgtta cggcgtacgc
     4981 ttactatagt tgtcacgagc tgaccccttg gttacgaata cagagtacaa accccgtaca
     5041 aaagtacggg gcataatgca attatagatg ggggggattt tgagggtttc aacccccggc
     5101 ggcgagccga atgcagtttg tatgacccgc catcttcaac cgattgaggg tgctgtctgc
     5161 gttctctttg cctttcaccg ggccaatgac cacacgattc cagccattgt tggtggtgat
     5221 tttcgagtca aatccttcga acgccagctg agcacgtacc gtctctgcct gttccgcgcc
     5281 tctgaacgaa ccgcactgca ccatccagcg gcgttcgtct tttttctccg ccgtcggttt
     5341 tggcgcgtca gcggcacgag caaccggcgc agcttgctgc ggcttcgact gcgcggtcgt
     5401 gtgcgcaggg gtttgcagca gatcctggta cggctgctga gtagaagctg gtttagactg
     5461 gcgcggttgc gcctgtaccg gcgcggcttg cgacgtacgc gtctgctgct gccagctttg
     5521 ttcagtcgta cgcgactgct gagccaggcg ttgctgctcc gctaattgct gcgcctgacg
     5581 ctggcgttgt agcgtttgct gacgctgttc cggcgtctgt tcattccacg gaacctcaac
     5641 cagctgcgtt ggctgctggc gcatatcagc ctgcatttgt tcaagaagct gacgttgttc
     5701 tggtgtcagt tgctccggcg ttttcacttc accaccggca gaaggttctg tgggcgcacg
     5761 cacacccggc tggcgacttt ccagctcttt aatgtagcgc cagcgttctt ctggttttgg
     5821 tggtagtccg tttccggtca ctttctggct ttgcagcgtc tcggactctt ctttcttgtg
     5881 atgcgtaatg aagtacagac caccgataaa ggtcacaaga acggcggcag caatagcgac
     5941 catagcggga gaaaccgcag gcagatttcg ttgctttttc cgtgaggtgc tcttttttcg
     6001 ccgcgaaggt gccggttggc tgcggcgtac ataatctcgt tgtgccacta tcgtttcgct
     6061 gtatttattc gttcgtcagc ccgccatgtt acttaagcgg cgggcctttg accagtcacg
     6121 gcagtcttaa aggtttactt taaggtaacg cgcgtgttga tccccggatg ataagttcgc
     6181 agtccattaa acgagagcca ctgccaacgt gttgcccctg catttgatca agcaataaca
     6241 gcatagcttc ccgaccgatt tcgtaacgcg gctgcgcgat ggttgtcagc ggcggatcac
     6301 aaaattgcgt caggtcgatg ttatcaaaac cgattatgga aaggtcttcc gggactttca
     6361 gcccctggcg ttttgcctga gaaagtgcgc cgagcgccat cacatcgcta tggcagaaga
     6421 ctgcagtagg cggttgtgga agatcaagca gctgttgcat tgctttgctt ccggcttcga
     6481 aggtgaagtc gccacgggcg atgtattgtg gatcaaccat aattccgcag cgacgcagcg
     6541 cctgaacata gccttgcagg cggtagtgac acagtggcat ctcttcggga ccggctatac
     6601 agccaatccg tttatgccct tgctcatata aataatttac tgcatcaaat gcggcggtca
     6661 gattgtcgat atgaacggta ggcagctcca gttccggtgc aaattcgttc gccatcacca
     6721 tcggcggcag attacgctgt tcctcaatgc tggcatcaaa cggcagcctt gaacccagca
     6781 gcaacatgcc atcaatttgc ttggtgatga tcaaatcgat aaaggttttt tcctgctgat
     6841 tttgatgcgc acagtcgcca atcagcacca gatatccgtg atttgccgcc gtaacttcga
     6901 taccgcgaat aatttcgcta aagaagggat cgcagatatc cgggacaatc accagaatgg
     6961 tgcgggattc attacgcttg acgttgcgcc ccataggctg cggtaaataa cccacttccc
     7021 gggccgcttt ttcaacccga ttacgggtgg cctgggagac tttatcggga ttcattaatg
     7081 ctcgggagac agtcgctgta gagacttttg ccttgagggc aacgtctttc atggtcgcgg
     7141 cagtttcctg cttcttcgct ttcacactca ctcctcgcct ggcacgtcag gcgtactaca
     7201 tccatgttta cttcacatcg gcaacatttt tagcagatag cgcgtgaaaa cggttacaga
     7261 attttcatga aaagtgtgat gaatattgaa tttttcgatc cgcctcgcat cgtaagcggt
     7321 ttaaccctca atcggatcaa catccagtac ccatttcacc ttgcgggaat ccggtattgt
     7381 attgatgagc gccagcgtac cactaatgat gtgttgcaag cgcacgcggg aagggtgctg
     7441 caacaatatc tgccagcgcc agcggccgcc acgtttaggt gccagtgccg gaaccggacc
     7501 gagaacccac agtttgtcgt ctgccagcgg actggagagg atcagattac gcagttgttg
     7561 caggaacagc ggcgcatgct gattgttatg atcttccgca cgcacaatca catggctggt
     7621 ccatggcggc agttgcatca tccgtcgctc agccagcgcc tgttcggcaa aggcgtcgta
     7681 gcctttatag agcaacgttt gcaacagagg atgttccgga tggtgcgttt gcagcaccac
     7741 ttcgccttgt ttacccgcac gcccggcacg accagcgacc tgggtgtaaa gctgagcgaa
     7801 acgctctgcc gagcgaaaat cggcagaaaa cagcgcgccg tccacatcca gtaacgccac
     7861 cagcgtcaca tccggaaagt ggtgaccttt cgccagcatt tgtgtgccga tcaaaatcct
     7921 cgcaccgccg cgatgtactt ccgccagttg ctgttccagc gcccctttgc ggctggtggt
     7981 atcgcggtcg atacgagaaa tgggcacatc agggaacagc ggcgcaagcg tctgttcaag
     8041 ctgttcggtg cccagcccca cgggtaccag gtgcgtggaa ccgcaggaag ggcactggcg
     8101 cggcaccgga cgctgactgt cacagtggtg acagcgcaaa tgctgttgcg cctgatgcag
     8161 cgtgtagtag tgatcgcaac gtgggcattc ggcaatccag ccgcagtcgt ggcacagtag
     8221 cgcaggcgca aagccacggc ggttaagaaa gaggataacc tgattgttgg cctgtaaatg
     8281 ctggcgcata cgggtgatta acgccggagc cagacctgcc tgcaccttct gaccttttaa
     8341 atccagcacg tgttgaattg ccggacgcgc attccccgcc cggcgcgtca ggcgcagcag
     8401 gcggtatttt ttctgctgga cgttgcacaa cgtttccagc gcgggcgttg cggagccaag
     8461 aataatcggg atttgctcgc tatgcgcacg atacaccgcc aggtcgcggg catgatagcg
     8521 ccagccttcc tgctgcttgt aggaactgtc gtgctcttcg tcaatgacaa tcacgccgag
     8581 atttttaaac ggcgtaaaca gcgcggagcg ggtgccgatc acaatcgccg cctcgccatt
     8641 tttcgctttc agccacgccg aaagacgctc gctgtcgttc agaccggaat gcagaacttc
     8701 cacgggcgca ttaaaccgtt cacgaaaacg ggcgatagtt tgcggtgtca ggccgatttc
     8761 cggcaccatc accagcgcct gtttgccctg agcgagcacg ttttccagta cactgagata
     8821 aacctccgtt ttaccggaac cggtaacgcc cgccagcagc caggcagaaa aagtatctgc
     8881 cgcgctatga attgcgccaa cggcggtggc ctgttcggta ttcaatcgca accgctcacc
     8941 agaaacggca tagttcgttc gccagtcgct aaactctggt gtttcacttg ctaaatcaca
     9001 cagacctttt ttgcgcagcg cctgcaacgc ggcatcatta aattcgagcg tggcaacctg
     9061 gtcgcgccag attttgcctt gccgtaacgc tgccagcgcc tgttgttgct tgggtgagcg
     9121 tttcaggctg ttcagatcca ccgcctgacc ttgttcagtg gcaaaccagt accacatcgg
     9181 cgcgttcgcc gcaggccgcc cctggcgtag taaaatcggc aaggcatgaa acagcacatc
     9241 gccaatcgga tgatgatagt aatctgccgc ccatagcagc aatcgccaga cggagtgagt
     9301 aaacaccggc tcaccatcca gcacttcgac taccgctttt agctcagtga gcggcagttc
     9361 gctggcatcg ctaactgata ccacaatccc gatgcgctcc tgctgtttgc caaacggcac
     9421 gcgcacgcga cacccagctt taaccgtcat gccttctggc agcagatagt caaaggtacg
     9481 aggaagcgga acgggcaagg caacgtgggc aacgggcata gcatcatcct gacttgaaat
     9541 tcggtgggtt agtatacaca ttgccgtaga agagtgcgga tcagtttgca tacgctggtt
     9601 aatttctgta tgatttcgcg ccttcgtacg aaatgatcgt attgaagcta tacttttaac
     9661 atcgcgtggt gtctggcgtt agggctggaa gagcgacgcg gccttaaacc gaggttttcc
     9721 catgaaaaaa gatattcacc cgaaatacga agaaattact gctagctgct cttgcggtaa
     9781 cgtaatgaaa atccgctcca ccgttggtca tgacctgaac ctcgacgtgt gcagcaagtg
     9841 ccacccgttc ttcactggca aacagcgtga tgttgctacc ggtggccgtg ttgaccgctt
     9901 caacaagcgt ttcaacatcc cgggcagcaa ataagaccgg atttccgaaa aaagcgccgc
     9961 acggcgcttt ttttgtgcct gaattttggt cgtatcacca cgaaaacagc ggccactctt
    10021 tggcaaccgt ggtgagttgt ggcgcgtcga aaacggcttg tggtgaaact accgtttcct
    10081 caagcggaat atttttaccg tagcgttctt tgagcttcgc ctggaccagc gggttgctga
    10141 gatcaaactc tttcaacttc tgctgttcgc ccacgcgcat tcccagcgcg ttctggtaaa
    10201 ccttccacac cacttccgaa cagtactggc gatcgtcgct ccatgagaag ctgaaatcat
    10261 acggtttacc gagataacgt tttgccgttt gcgtcagttt ttgctgttgt tcaacactca
    10321 gtccgccttc aacgcggcga acaacgtagt tgcccttttc accatgggca atccactgct
    10381 tgagcggggt gtatttcacc gggccgaccg cttcaaaaac gtagggcttt ttgttacgca
    10441 tcaccagcat accggtgtgg ctgtaatcgg aatgggttgc cagttggatc gctttacttt
    10501 gcgatgagcg agagatttga aagatgatgt cgccagtttg cggctgccag gcaaaggcag
    10561 gtacagaaac cagcaggctg aggatcagca gcctgttttt catagttaaa cgtccatgta
    10621 taaaaagcgg tgggtcgcag acaacgtgct cgttgtttat gccggatgcg gcgtgaacgc
    10681 cttatccggc ctacaagttc gtgcaaattc aataaattgc aatatgacgt aggcctgata
    10741 agcgtagtgc atcaggcgat tccactccgc gccgctcttt tccgctttag tattcccacg
    10801 tctccgggtt aatccccatc tcacgcatga tctcttttgc cgcttccggg atttcgtcgc
    10861 tgcgctcttt acgcagatcg gcatcatccg gcaaaggttg cccggtaaag gcatgcagaa
    10921 acgcttcgca cagcagctcg ctgttggtag cgtgacgcag gttgttcacc tgacgacgcg
    10981 tgcgttcatc ggtgaggatt tttaacacct taagaggaat ggaaaccgta atctttttga
    11041 cttgttcact cttcttgccg tgttcagcgt atgggctgat atattcgccg ctccattcag
    11101 ccatgagata cttaatcctc ttcgtcaata aattgagacc agaccccaca atttatgtgg
    11161 gtactgaccg taaacccgca tagtttaccg tacaggcgtt accgtgacat cgtgtaatgc
    11221 acctgtcggc gtgataatgc atataatttt aacggctatt tgggatttgc tcaatctata
    11281 cgcaaagaag tttagatgtc cagatgtatt gacgtccatt aacacaatgt ttactctggt
    11341 gcctgacatt tcaccgacaa agcccaggaa acttcatcac atgacgcgta aacaggccac
    11401 catcgcagtg cgtagcgggt taaatgacga cgaacagtat ggttgcgttg tccctcccat
    11461 ccatctttcc agcacctata actttaccgg atttaatgaa ccgcgcgcgc atgattactc
    11521 gcgtcgcggc aacccaacgc gcgatgtggt tcagcgtgcg ctggcagaac tggaaggcgg
    11581 tgctggcgca gtacttacta ataccggcat gtccgcgatt cacctggtaa cgaccgtctt
    11641 tttgaaacct ggcgatctgc tggttgcgcc gcacgactgc tacggcggta gctatcgcct
    11701 gttcgacagt ctggcgaaac gcggttgcta tcgcgtgttg tttgttgatc aaggcgatga
    11761 acaggcatta cgggcagcgc tggcagaaaa acccaaactg gtactggtag aaagcccaag
    11821 taatccattg ttacgcgtcg tggatattgc gaaaatctgc catctggcaa gggaagtcgg
    11881 ggcggtgagc gtggtggata acaccttctt aagcccggca ttacaaaatc cgctggcatt
    11941 gggtgccgat ctggtgttgc attcatgtac gaaatatctg aacggtcact ctgacgtggt
    12001 ggctggcgtg gtgattgcta aagacccgga cgttgtcact gaacttgcct ggtgggcaaa
    12061 caatattggc gtgacgggcg gcgcgtttga cagctatctg ctgctacgcg ggttgcgaac
    12121 gctggtgccg cgtatggagc tggcgcagcg caacgcgcag gcgattgtga aatatctgca
    12181 aacccagccg ttggtgaaaa aactgtatca cccgtcgctg ccggaaaatc aggggcatga
    12241 aattgccgcg cgtcagcaaa aaggctttgg cgcaatgttg agttttgaac tggatggcga
    12301 tgagcagacg ctgtgtcgtt tcctgggcgg gctgtcgttg tttacgctgg cggaatcatt
    12361 agggggagtg gaaagtttaa tctctcacgc cgcaaccatg acacatgcag gcatggcacc
    12421 agaagcgcgt gctgccgccg ggatctccga gacgctgctg cgtatctcca ccggtattga
    12481 agatggcgaa gatttaattg ccgacctgga aaatggcttc cgggctgcaa acaaggggta
    12541 aaaatgagtg tgattgcgca ggcaggggcg aaaggtcgtc agctgcataa atttggtggc
    12601 agtagtctgg ctgatgtgaa gtgttatttg cgtgtcgcgg gcattatggc ggagttctct
    12661 cagcctgacg atatgatggt ggtttccgcc gccggtagca ccactaacca gttgattaac
    12721 tggttgaaac taagccagac cgatcgtctc tctgcgcatc aggttcaaca aacgctgcgt
    12781 cgctatcagt gcgatctgat tagcggtctg ctacccgctg aagaagccga tagcctcatt
    12841 agcgcttttg tcagcgatct tgagcgcctg gcggcgttgc tcgacagtgg tattaacgat
    12901 gcggtgtatg cggaagtggt gggccacggt gaagtgtggt cggcgcgcct gatgtctgcg
    12961 gtacttaatc aacaagggct gccagcggcc tggcttgatg cccgtgagtt tttacgcgct
    13021 gaacgcgctg cgcaaccgca ggttgatgaa gggctttctt acccgttgct gcaacagcta
    13081 ctggtacaac atccgagcaa acgtctggtg gtgaccgggt ttatcagccg taacaacgcc
    13141 ggtgaaacgg tgttgctggg gcgtaacggt tccgactatt ccgcgacaca aatcggtgcg
    13201 ctggcgggtg tttctcgtgt aaccatctgg agcgacgtcg ccggggtata cagtgccgac
    13261 ccgcgtaaag tgaaagatgc ctgcctgctg ccgttgctgc gtctggatga ggccagcgaa
    13321 ctggcgcgtc tggcggctcc cgttcttcac gcccgtactt tacagccagt ttctggcagc
    13381 gaaatcgacc tgcagctgcg ctgtagctac acgccggatc aaggttccac gcgcattgaa
    13441 cgcgtgctgg cctccggtac tggtgcgcgt attgtcacca gccacgatga tgtctgtttg
    13501 attgagtttc aggtgcccgc cagtcaggat ttcaaactgg cgcataaaga gatcgaccag
    13561 atcctgaaac gtgcccaggt gcgtccactg gcggttggcg tgcataacga tcgccagttg
    13621 ctgcaatttt gctacacctc agaagtggcc gacagtgcgc tgaaaatcct cgacgaagcg
    13681 ggattacctg gagaactgcg cctgcgtcag gggctggcgc tggtggcgat ggtcggggcg
    13741 ggtgtcaccc gtaacccgct gcattgccac cgcttctggc agcaactgaa aggccagccg
    13801 gtcgagttta cgtggcagtc cgatgacggc atcagtctgg tggcagtact gcgcaccggc
    13861 ccgaccgaaa gcctgattca ggggctgcat cagtccgtct tccgcgcaga aaaacgcatc
    13921 ggcctggtat tgttcggcaa aggcaatatc ggctctcgct ggctggaact gttcgcccgc
    13981 gagcagagca cgctttcggc gcgtactggc tttgaatttg tgctggcagg cgtggtggac
    14041 agccgccgca gtctgttgag ttatgacggg ctggacgcca gccgcgcgtt agccttcttc
    14101 aacgatgaag cggtcgagca ggatgaagag tcgcttttcc tgtggatgcg cgcccatccg
    14161 tatgatgatt tagtggtgtt ggacgttacc gccagccagc agcttgctga tcagtatctc
    14221 gatttcgcca gccacggttt ccacgtcatc agtgccaaca aactggcggg agcaagcgac
    14281 agcaataaat atcgccagat ccacgacgcc ttcgaaaaaa ccgggcgtca ctggctatac
    14341 aacgccaccg ttggtgcggg cttgccgatc aaccataccg tgcgcgatct gatcgacagc
    14401 ggcgatacca ttttgtcgat cagtgggatc ttctccggca cgctctcctg gctgttcctg
    14461 caattcgacg gtagcgtgcc gtttaccgag ctggtggatc aggcgtggca gcagggctta
    14521 accgagcctg atccgcgtga cgacctctcc ggcaaagacg taatgcgcaa gctggtgatt
    14581 ctggcgcgtg aagcgggtta caacattgaa ccggaccagg tacgtgtgga atcgctggtg
    14641 cctgctcatt gcgaaggtgg cagcatcgac catttctttg aaaatggcga tgaacttaac
    14701 gagcagatgg tgcaacggct ggaagcggcc cgcgaaatgg ggctggtgct gcgctacgtg
    14761 gcgcgtttcg atgccaacgg caaagcgcgt gtgggcgtgg aggcggtgcg tgaagaccat
    14821 ccgctggcat cactgctgcc atgcgataac gtctttgcca tcgaaagccg ctggtaccgc
    14881 gataatccgc tggtgatccg tgggcctggc gctgggcgcg acgtcaccgc cggggcgatt
    14941 cagtcggata tcaaccggct ggcacagttg ttgtaatttc agaaatttaa taatgcccgg
    15001 tactcatgtt ttcgggtcta tggtttctaa tgaaatatat tgaattatca tagaattagg
    15061 ccggatgaag cgtttacaac gaatccggca agaagcaata agtacatggt tagtttatat
    15121 ttgcagtccg gtttgctttg catatcggat tttctttttc ttaccatcct gaagtttttt
    15181 catcttccct gatttttcct caccatcatt ggtcattttt cggttgacgc ccttcggctt
    15241 ttccttcatc tttacatctg gacgtctaaa tggatagatg tgcacaacac aacatataac
    15301 tacaagcgat tgatgaggta aggtatgagc ttttttcacg ccagccagcg ggatgccctg
    15361 aatcagagcc tggcagaagt ccaggggcag attaacgttt cgttcgagtt tttcccgccg
    15421 cgtaccagtg aaatggagca gaccctgtgg aactccatcg atcgccttag cagcctgaaa
    15481 ccgaagtttg tatcggtgac ctatggcgcg aactccggcg agcgcgaccg tacgcacagc
    15541 attattaaag gcattaaaga tcgcactggt ctggaagcgg caccgcatct tacttgcatt
    15601 gatgcgacgc ccgacgagct gcgcaccatt gcacgcgact actggaataa cggtatttgt
    15661 catatcgtgg cactgcgtgg cgatctgccg ccgggaagtg gtaagccaga aatgtatgct
    15721 tctgacctgg tgacgctgtt aaaagaagtg gcagatttcg atatatccgt ggcggcgtat
    15781 ccggaagttc acccggaagc aaaaagcgct caggcggatt tgcttaatct gaaacgcaaa
    15841 gtggatgccg gagccaaccg cgcgattact cagttcttct tcgatgtcga aagctacctg
    15901 cgttttcgtg accgctgtgt atcggcgggc attgatgtgg aaatcatccc gggcattttg
    15961 ccggtatcta actttaaaca ggcgaagaaa tttgccgaca tgaccaacgt gcgtattccg
    16021 gcgtggatgg cgcaaatgtt tgacgggctg gatgacgatg ccgaaacccg caaactggtg
    16081 ggcgcgaata ttgccatgga tatggtgaag attttaagcc gtgaaggagt gaaagatttc
    16141 cacttctata cgcttaaccg cgctgaaatg agttacgcga tttgccatac gctgggtgtt
    16201 cgacctggtt tataaatagt gtggcttttg tgaaaatcac acagtgatca caaattttaa
    16261 acagagcaca aaatgctgcc tcgaaatgag ggcgggaaaa taaggttgtc agccttgttt
    16321 tctccctcat tacttgaagg atatgaagct aaaacccttt tttataaagc atttgtccga
    16381 attcggacat aatcaaaaaa gcttaattac gatcaatttg atctacatct ctttaaccaa
    16441 caatatgtaa gatctcaact atcgcatccg tggattaatt caattataac ttctctctaa
    16501 cgctgtgtat cgtaacggta acactgtaga ggggagcaca ttgatgagca cgtcagacga
    16561 tatccataac accacagcca ctggcaaatg cccgttccat cagggcggtc acgaccagag
    16621 cgcgggggcg ggcacaacca ctcgcgactg gtggccaaat caactccgtg ttgacctgtt
    16681 aaaccaacat tctaatcgtt ctaacccgct gggtgaggac tttgactacc gcaaagaatt
    16741 cagcaaatta gattactacg gcctgaaaaa agatctgaaa gccctgctga cagaatctca
    16801 gccgtggtgg ccagccgact ggggcagcta cgccggtctg tttattcgta tggcttggca
    16861 cggcgcaggt acttaccgtt caatcgatgg acgcggcggc gcgggtcgtg gtcagcaacg
    16921 ttttgcaccg ctgaactcct ggccggataa cgtaagcctc gataaagcgc gtcgcctgtt
    16981 gtggccaatc aaacagaaat atggtcagaa aatctcctgg gccgacctgt ttatcctcgc
    17041 aggtaacgtg gcgctggaaa actccggctt ccgtaccttc ggttttggtg ccggtcgtga
    17101 agacgtctgg gaaccggatc tggatgttaa ctggggtgat gaaaaagcct ggctgactca
    17161 ccgtcatccg gaagcgctgg cgaaagcacc gctgggtgca accgagatgg gcctgattta
    17221 cgttaacccg gaaggcccgg atcacagcgg cgaaccgctt tctgcggcag cagctatccg
    17281 cgcgaccttc ggcaacatgg gcatgaacga cgaagaaacc gtggcgctga ttgcgggtgg
    17341 tcatacgctg ggtaaaaccc acggtgccgg tccgacatca aacgtaggtc ctgatccaga
    17401 agctgcaccg attgaagaac aaggtttagg ctgggcgagc acttacggca gcggcgttgg
    17461 cgcagatgcc attacctctg gtctggaagt agtctggacc cagacgccga cccagtggag
    17521 caactatttc ttcgagaatc tgttcaagta tgagtgggta cagacccgca gtccggctgg
    17581 agcaatccag ttcgaagcgg tagacgcacc ggaaattatc ccggatccgt tcgatccgtc
    17641 gaagaaacgt aaaccgacaa tgctggtgac cgacctgacg ctgcgttttg atcctgagtt
    17701 cgagaagatc tctcgtcgtt tcctcaacga tccgcaggcg ttcaacgaag cctttgcccg
    17761 tgcctggttc aaactgacgc acagggatat ggggccgaaa tctcgctaca tcgggccgga
    17821 agtgccgaaa gaagatctga tctggcaaga tccgctgccg cagccgatct acaacccgac
    17881 cgagcaggac attatcgatc tgaaattcgc gattgcggat tctggtctgt ctgttagtga
    17941 gctggtatcg gtggcctggg catctgcttc taccttccgt ggtggcgaca aacgcggtgg
    18001 tgtcaacggt gcgcgtctgg cattaatgcc gcagcgcgac tgggatgtga acgccgcagc
    18061 cgttcgtgct ctgcttgttc tggagaaaat ccagaaagag tctggtaaag cctcgctggc
    18121 ggatatcatc gtgctggctg gtgtggttgg tgttgagaaa gccgcaagcg ccgcaggttt
    18181 gagcattcat gtaccgtttg cgccgggtcg cgttgatgcg cgtcaggatc agactgacat
    18241 tgagatgttt gagctgctgg agccaattgc tgacggtttc cgtaactatc gcgctcgtct
    18301 ggacgtttcc accaccgagt cactgctgat cgacaaagca cagcaactga cgctgaccgc
    18361 gccggaaatg actgcgctgg tgggcgggat gcgtgtactg ggtgccaact tcgatggcag
    18421 caaaaacggc gtcttcactg accgcgttgg cgtattgagc aatgacttct tcgtgaactt
    18481 gctggatatg cgttacgagt ggaaagcgac tgacgaatcg aaagagctgt tcgaaggacg
    18541 tgaccgcgaa accggcgaag tgaaatacac agccagccgt gcggatctgg tatttggttc
    18601 taactctgtc ctgcgtgcag tagcggaagt ttacgccagc agcgatgccc acgagaagtt
    18661 tgttaaagac ttcgtagcgg catgggtgaa agtgatgaac ctcgaccgtt tcgacctgct
    18721 gtaatctgac cttcttcagc gactgtcttt caggcagtcg ctgaagtttc tttaccggcg
    18781 tatagtgtcc acaggaaaac tacacactgg atctctcatg tctgccgcag gaaagagcaa
    18841 cccactggca atcagtggcc tggttgtgct cacacttatc tggagttata gctggatttt
    18901 catgaagcaa gtcaccagtt acatcggtgc cttcgacttt accgccttac gctgcatttt
    18961 cggcgctctc gttttattca tcgtcctttt attacgtggt cgcggaatgc gcccgacgcc
    19021 gtttaaatac accttagcca ttgccctgtt acaaacctgc gggatggttg gtctggcgca
    19081 gtgggcgttg gtcagcggag gtgcggggaa ggtggcgatc ctgagctata ccatgccgtt
    19141 ctgggtggtg attttcgccg cgttgtttct cggtgaacgc ctgcgacgtg ggcaatattt
    19201 cgcgattctg attgccgctt tcggcttatt tttggtgttg cagccgtggc aactcgattt
    19261 ctcttcgatg aaaagtgcca tgctggcaat cctctccggt gttagttggg gggcgagcgc
    19321 gattgttgct aaacgcctat acgcccgtca tccgcgcgtg gatttattat cgttaacatc
    19381 ctggcagatg ctgtatgcgg cgctggtgat gagtgtggtc gctttactgg tgccgcaacg
    19441 tgaaattgac tggcagccca ccgtgttctg ggcgctggcc tacagtgcga ttctggcgac
    19501 ggcgctagcg tggagcttat ggctgtttgt gctgaaaaac ttgcctgcca gtattgccag
    19561 cttaagcaca ctggccgttc ccgtttgtgg cgtactcttt tcctggtggc tgctcggtga
    19621 gaatccgggg gccgttgaag gtagcggtat tgtgctgatt gtgctggcac tggcgctggt
    19681 gagccgtaag aaaaaagaag ctgtcagtgt aaaaagcatc tgaatttttt cttcatgtgg
    19741 ggcgatctct tatttaacaa aataacgata atgccccacc atccgccagc taaacagcac
    19801 atcttcttcc tgcgcgcctg cgccaatgtt atgtatcacc agcggcgtac cgtcgcgggc
    19861 gaagccatct gacgccaccc caatatgcgc cagcccgtta tctaatcgcc aggagacaat
    19921 atcgcccgct tgatagtcac cggcattttt actgatgggg cgtgttttgt tgtggcggct
    19981 aaaccaggtt tccagattag gcacccgacg gtgatcgatg ttgctgtccg ggcgctttaa
    20041 cttccatttt tgcgggtact cagcaaaatt cttcgccata tcttcgtgaa ccagtttctg
    20101 caaatcgacc ttctggctgc gcaatgcgcg gatcaccaca tcggaacata caccgcgttc
    20161 ttgcggaaca tcaccgccag gataggtaag ctgcatatac gccggatcgt aaaatagcgt
    20221 gctgccaatt tgctgtctgg caccgtctgc gatggcaagg ttggtattgg cctggatttg
    20281 taccacggtt ggtggaacgg cgggagattt taaggagtgg ctggtaaatg ccgttagcag
    20341 gctgagcagc gccagtgaag ctttcatctg gttcgtccgt gaaaataaga gtcacagatt
    20401 cgaccttccg ggctaacgat tgttctgtgg agaaaatgtc cagaagcgga taaaaaactc
    20461 ctgaacaacg tgggagtttc agattactgg cacagagtgc cggatgcgga gtgaacgcct
    20521 tatccggcct acaaaagcac gcaaattcaa tatattgcag agattgcgta ggcctgataa
    20581 gcgtagcgca tcaggcaatt ttgcgttcaa actcccggac aagccgggag tttggagtag
    20641 gttattccca ctcttgcagg aaacgctgac cgtactggtc agctaccagc agtgcggcgt
    20701 aaacctgatc tggcgtcgcg ccgccaggca tgttgtggat ggtttcacct tctgcacaag
    20761 atgcttttgc aacaatgcgc attttcgccg ggacatcttc tttaatatcc agttgagcga
    20821 gagttattgg caaacctacc gcatggctaa gtgcagctac ggtttcgatt tcctccaccg
    20881 gcgcgttttc cagtaccagc tgcgtcagcg taccaaatgc cactttttca ccgtgataat
    20941 agtgatgcgc gtccgggata gcggtcaggc cgttatgcac cgcgtgagcc gccgctttca
    21001 aaaccaacgc cgctcaaata ggtgttcgct tcaatcacgc gctccagcgc cggggttact
    21061 acatgctgtt cggcggcaag catcgctttt tcgccttctt ccagcagggt gttgtagcac
    21121 agttcagcca gtgccagcgc cgcctgggtg cacttgccgc ccgccatggt ggtcgcgccg
    21181 ctacgagagc aagcacgcgc ttcaaaccag gttgccagcg catcgccgat acccgctgct
    21241 aacagacgtg caggtgcgcc cgcgacgatt ttggtgtcga caatgaccat attcgggtta
    21301 tttggcaaca gcagatagcg gtcaaactca ccctcatcgg tgtagataac agacaatgcg
    21361 ctgcacggtg catcggtaga ggcgatagtc ggtgcgatcg ctaccggaac acccatgaaa
    21421 tgtgccagtg ctttggcagt atcgagggtt tttccgccac cgataccgag aattgcgcca
    21481 cactgcgcag tctccgcgat gccacgcaga cggtcgatct cattttgcga acattcaccg
    21541 ccaaacggcg taatttctac taccagtcca gcatctttaa agcttttctc gacagtggat
    21601 tgagcaaaac ctaaaacaaa tttgtcaccc accactaacc agcgttctgc cagcggcttc
    21661 aggtattcgc ccagacgatt aatcacatca gcgccctgga tgtatttacc cggtgattga
    21721 ataatgcggt ccataattgc tcctttagag atgagtagtg ccaaatgcgg cattccagtc
    21781 gtgttcgaac ttctctatag ctgactctac tgcaggggtg ttgagcattt gttgcgctac
    21841 atctaagggc agggtgatgg attcacatcc tgccagtaaa cagtccagcg cctgacacgg
    21901 cgttttaaag ctggctgcca gcaccatgct ttctggcgcg tgcatttcta acagcgtttg
    21961 cagctcctga accgtacgaa tgccgtcccc gccctgggca tctacgcggt taacatacgg
    22021 agcaacgtat tttgcccctg ccagtgcggc taataaccct tctgcggcgc tatatacagc
    22081 ggtgccaagt gtagtaatac cctctttttt cagtatttta attgctgcca gaccttcgga
    22141 agtcaccggg attttcacca caatacccgg aatagcgtcg cgcaggcgct tcgcttcttc
    22201 caccatcccc tgcgcgtcgc ggctcatggt ctgagcaaac agaatgccct catcaccaat
    22261 cgctttttgc agacgcggca gcacttccca gatggactcc ttgctggcag cgataatgct
    22321 cgggttagtt gtcaccccgg caatcgggaa tatgcgtgcc agacgttcga cttctgcgac
    22381 gttagcggtg tccagataca gttccatgat gtttcctttt acagttccag ttcatgttgc
    22441 agcaggctgg cgatagcgtc ggcagaggcg gcgttaacca gcgcgttacg gaattcttcg
    22501 tgcatgatgc ggcgagcgag gcgcgagaaa atgcgcatgt gctgatcgcc cgcagcgtgt
    22561 ttgttcaggg ttaacataat gatgaattgc gcttcatcat cgccccagcg caccggcgct
    22621 tgcagacgcg ccacgctgat ggtggattgc tcaatgtgtt ctgatttgct gtgtggaatg
    22681 gcaaaactaa agcccagacc ggtagagaaa acggcctcgc gcgcccataa gtcggcttcc
    22741 agtttacgcg gatagcggca gcggcccgcc agcagcaggt tatcggtcat gcctttgagc
    22801 acttcttctt tgctgcgcca gtcactttcc agcgtgatgc actcggcggt gaccagcggt
    22861 gcgtcctgtt gggtcatgcg gaattgcgcc agcaggtgtt ccacttccag cgaagtacgg
    22921 caggccattg cctggttgag caacttgcgg cactcacggc tatcaagttg cgccatccgc
    22981 gctttcgccg ccggaattga tggtgcgctc atgctgagtt catccagccc taagccgacc
    23041 agcaacggca gcacggaacc tttcgctccc agctcaccgc acagaccaat ccatttaccc
    23101 tggcgatgca ccgcctgcac ggcgtaatcg agcgcccgca agaatgccgg attcaggctg
    23161 ttgtagtgac gagtaacctt agcgttatcg cgatccactg ccagcagata ttgcgtcagg
    23221 tcattactac caatactaaa gaaatcaatc tcttcgcagc attgatcgat gatgaacatc
    23281 accgacggca cttccagcat aatgccgagc tggatcttct catcaaacgg aatgtgttcg
    23341 ttacgtagtt gctgtttggc ttctgccagt ttttctttca cccataagat ctcttccatt
    23401 gaggagatca tcgggatcat gattttcagg ctgccgtgag cggaggcgcg caggatcgag
    23461 cgtagttgtg tggtaaataa cgacgcgtat tcttcataaa tacgcacggc gcgataaccg
    23521 aggaacgggt ttgcctctgc gggaatgttt agataatcaa cgggtttgtc gccgccaatg
    23581 tccatagtgc gcacaataat gctgcgtccg ttggcagact ccagcgcctg acaaaaaatg
    23641 ttgtacagct cgctttcgcc cggtgcactg gtgcgatcca tatagagcat ttcagtgcgg
    23701 aacaaaccaa cgccttccgc accattgccg aatgcggcct gcgcttccac ggagtgagcg
    23761 atgttagcgg caatttcaat gcggataccg tcagcggtac gggcttgttg ggtcagccag
    23821 acacgctgtt gctcacgcag ggcgtcctgt acgcgggctt cttgctgata ataacgagct
    23881 acggcttctc ctggctcaac cacaatcgcc ccggcgttgc cgtcgatata aatcgtttga
    23941 tgctgccacg gagtgagggc atcaatatcc acaccaacca gcgtcggaat gttgaacgaa
    24001 cgggcaagga tcaccgtatg tgaggtggtg ccgccgcttt tcagtaacaa tcctttgagg
    24061 tgatttttat ccagttcgag gaactggctg ggggtcagtt catcagccat acaaatggcg
    24121 gactgcgtca gtttgcccgg tgccgggaag cgttgctcac cgtagatttg ctggagtaac
    24181 tggaagcaga cgtcgcgtac gtccagggca cgttcttgca ggtatctgct gctggaacgg
    24241 gcgaactctt cgcaaaagtg attcgcactg gcgacaatcg cttcggcgca gcttaatccg
    24301 gcgctgactc ctgccagtaa atgttcgtgc agggaagtat cgccagccag ggatcggtgc
    24361 gcttccagaa tcgcgctggt agcaccgtcg ctatccagca gacgaaactc aatgtttttc
    24421 agtaccagcg tcaggccgtt ttccagtgcg gattgctcgg cgtcaacgtc tttggctgcg
    24481 ggaagattac tcagcgcatt gagatctaaa gaagagatcg gcgtcagaat gccgcctgca
    24541 ctaccgctgc acacggtgcg gacgcggata atttgcggat tcagattggt cagtgaaacc
    24601 ggcagtggtt ccagttcgtc agatttaact tccgccagcg gcgcatcgca gtgggggaat
    24661 tcatcgcgca gccattggct taaacgctgg tgcccttcct gttcgtcggc cccggaaatc
    24721 agtagctggc agttatcacc caccagcgta tcggtgccaa tcagcgccag agcgcttttg
    24781 gcgttgcctt tacggtcagt acgcaggtta tgccactcaa tttgagatga aaaagtatta
    24841 cacagcgttt caacgtggct tgccggacgc gcatgtacgc cgttaggtag ctcacaaata
    24901 aattccacaa tcagggccat tgcctttctc ccggtacggt tttctgttta caggataaga
    24961 gcgcacggta acggcctgcc atgtgacaaa tctgccaaaa gctggacaaa tgtaatgtaa
    25021 ccgtcaattt gcgacgcgtc tcacaagacg ccgttttgcg gcatgcttcc ggattatcgc
    25081 aagttatgag gcggatcgca tttttgtact gatattacaa aagtccagta aatggccttt
    25141 ttatccactg tttgctccgc ctgcgattgc ctattgtttc ctccatcaaa aatatggaca
    25201 tgggcttttg caagcccggg agcagcttat gaatgagttg gtgcagatcc tgaaaaatac
    25261 ccgtcagcat ttaatgacgg gcgtttcaca catgattccc ttcgtggtat cgggcggtat
    25321 tttgctggcg gtttccgtca tgttgtatgg caaaggcgca gtgccggatg ccgtagccga
    25381 tccgaatttg aaaaaactgt ttgatattgg cgttgcaggt ttgacgctga tggtgccttt
    25441 cctcgccgct tacattggtt actccattgc agagcgttct gcgctggctc cgtgcgctat
    25501 cggtgcctgg gttggtaaca gctttggtgc gggcttcttt ggtgcgctga tcgccgggat
    25561 tatcggcggc atcgtggtgc attacctgaa gaaaattccg gtgcataaag ttctgcgctc
    25621 ggtgatgccc atcttcatta ttcctatcat cggcacactg attaccgcag gcatcatgat
    25681 gtggggcttg ggcgagcctg taggggcgtt gaccaacagc ctgactcagt ggcttcaggg
    25741 gatgcagcag ggcagcattg ttatgctggc ggtgatcatg gggctgatgc tggcgttcga
    25801 tatgggcggt ccggttaaca aagtggccta tgccttcatg ctgatttgcg ttgctcaggg
    25861 tgtttatacc gtggtggcca ttgctgccgt gggtatttgt atcccgccgc tggggatggg
    25921 gctggcgacg ctgattggtc gtaaaaattt ctccgcagaa gagcgcgaaa ccggcaaagc
    25981 ggcactggtg atgggctgtg ttggcgttac tgaaggggcg attcctttcg ccgctgccga
    26041 tccgctgcgt gtcattcctt ccattatggt cggttcagtt tgtggtgcag taactgcggc
    26101 gctggtcggt gcgcagtgct atgcaggctg gggtggtctg attgtgctgc cggtggttga
    26161 aggtaagttg ggttatatcg cagcagtggc tgtcggagca gtggtgacgg ctgtttgtgt
    26221 gaacgtgctg aaaagtctgg cgcgtaaaaa tgggtcttcg actgatgaaa aagaagacga
    26281 cctggatttg gattttgaaa ttaattaatt gaggaacgag ccatgacgaa aattattgca
    26341 gtaaccgcat gtccttcagg tgttgcccat acctatatgg tagcagaggc gctggaaagt
    26401 gccgcgaaag cgaaaggctg ggaagtgaaa gtagaaaccc aggggtcaat tggtctggaa
    26461 aatgaactga ctgcggaaga cgtggcgagc gctgatatgg tgattctgac caaagatatc
    26521 ggcatcaagt ttgaagagcg ttttgctggc aagaccatcg tgcgcgtcaa catcagcgat
    26581 gcagtaaaac gtgctgacgc cattatgagc aaaattgaag ctcacctggc gcaaaccgct
    26641 taagttcttc tcccgctcgc aagggcgggt tcgctttctc acaggagttc ctcatgacga
    26701 atcgtatctc tcgcctcaaa actgcactgt ttgccaatac ccgtgaaatc tcgctggagc
    26761 gggcgctgct ttataccgcc agccatcggc aaaccgaagg cgaaccggtg atattgcgcc
    26821 gggcgaaagc aacagcgtat atccttgaac atgttgaaat ttcgattcgt gatgaagaac
    26881 tgattgccgg taaccgcacc gtaaaaccgc gcgccgggat tatgtcgccg gaaatggacc
    26941 cttactggct gctgaaagag ctggatcaat tcccgacgcg tccgcaggac cgctttgcta
    27001 tcagcgaaga agataaacgt atctaccgtg aagagttgtt cccgtactgg gaaaaacgtt
    27061 cgatgaaaga tttcatcaac gggcagatga cagatgaagt aaaagccgcg accaacacgc
    27121 agattttcag catcaaccag acggataaag gccaggggca cattattatt gattacccac
    27181 gcctgttgaa tcacggtctg ggtgagctgg tggcacagat gcagcaacat tgtcagcaac
    27241 agccggagaa tcacttttat caggccgcgt tgttactgct ggaagcctcg cagaaacaca
    27301 ttttgcgtta cgccgaactg gcggaaacga tggcggcaaa ctgcacagat gcccagcgtc
    27361 gcgaagagct gctgactatt gcggagatct cccgccataa cgcgcaacat aagccgcaga
    27421 cgttctggca ggcgtgccag ttattctggt acatgaacat cattctgcaa tacgaatcca
    27481 acgccagttc gctgtcttta gggcgcttcg accagtatat gttgccgttc tatcaggcat
    27541 ctttaaccca gggcgaagat ccggcgttcc tgaaagaact gctcgaatct ttatgggtga
    27601 aatgcaacga catcgtgctg ttgcgctcca ccagtagcgc gcgttatttc gccggtttcc
    27661 cgaccggcta taccgcactg ctcggcgggt taaccgagaa cggacgtagc gcggtgaacg
    27721 tgctttcgtt cctttgcctt gacgcctatc aaagcgtgca attaccgcaa ccgaacctcg
    27781 gcgtgcgcac taacgccttg atcgacacgc cgttcctgat gaaaaccgcc gaaaccattc
    27841 gcctcggcac cggtattccg caaatcttta acgatgaagt ggtggtgcca gcgttcctca
    27901 accgtggcgt ttcgctggaa gatgcgcgcg actattccgt agtgggctgt gtggaattat
    27961 ctattcccgg cagaacctac ggcttgcatg acatcgcgat gtttaatctg ctgaaagtga
    28021 tggaaatctg cctgcatgaa aatgaaggca atgctgcgct gacttatgaa ggtttactgg
    28081 agcagatccg cgccaagatc agccactaca tcaccctgat ggttgagggc agcaatattt
    28141 gtgatatcgg ccatcgcgac tgggcacctg taccgctgct ctcgtctttt atcagcgatt
    28201 gtctggaaaa aggtcgcgat attaccgatg gcggcgcgcg ttataacttc tccggcgtac
    28261 agggaattgg tatcgccaac ctgagcgatt ctctccatgc gttgaaaggg atggtttttg
    28321 agcaacagcg tttaagtttt gacgaattgc tgtcggtatt aaaagccaac tttgcaacgc
    28381 cagaaggcga aaaagtccgc gctcgcttaa ttaaccgctt tgagaaatac ggtaacgata
    28441 tcgacgaggt ggataacatt agcgccgaac tgttgcgcca ctactgcaaa gaagtggaaa
    28501 aataccagaa cccgcgcggc ggttacttca cgccgggatc gtataccgtt tctgctcacg
    28561 ttccgttggg atcggtggtt ggcgcgacgc cagacggtcg ttttgccggg gaacaactgg
    28621 cagacggcgg cttgtcacct atgctgggtc aggacgcaca agggccaacg gcggtactga
    28681 agtcagtcag taagctcgat aatacgctgc tgtctaacgg tacgttgctg aacgtgaaat
    28741 tcactccggc gaccctggaa ggtgaagcgg gattacgcaa actggccgac ttcttacggg
    28801 tgtttaccca gcttaagttg cagcatattc agtttaacgt ggtgaacgcc gacacgttgc
    28861 gggaagcgca acagcgccca caagattatg ccgggctggt ggtgcgcgtt gctggataca
    28921 gcgccttctt tgtcgaactg tcgaaggaga tccaggatga catcatccgc cggacagcgc
    28981 atcagctgta acgttgtgga aacgcgccgc gatgatgtgg cgcgcatttt caacattcag
    29041 cgttattcac tgaatgacgg cgagggcatt cgtacggtgg tcttttttaa aggctgtccg
    29101 catctttgcc cgtggtgtgc taatccggag tcgatctccg gcaaaatcca gacggtacgc
    29161 agagaggcga aatgtctgca ctgtgcgaaa tgtttgcgtg atgcggatga atgcccctcc
    29221 ggggcgtttg aacggattgg tcgcgatatc agccttgacg ctctggaacg ggaagtgatg
    29281 aaagatgaca ttttctttcg cacgtccggc ggcggcgtca cgctttctgg cggcgaagtg
    29341 ttaatgcagg cggagtttgc tacccgtttt ttacagcgac tgcggctgtg gggtgtctca
    29401 tgcgccattg aaactgccgg agacgcgcca gcaagcaagc tgttaccgct ggcgaaattg
    29461 tgcgatgaag tgctgttcga tttaaaaatt atggacgcga ctcaggcgcg ggatgtggtg
    29521 aagatgaacc tgccacgcgt gctggagaat ctgcgtttgc tggtgagtga gggtgtcaac
    29581 gtgatcccgc gtttaccgtt gatccctggt ttcacgctca gccgggagaa tatgcagcag
    29641 gcgctggatg tactgatccc gctgaaaatc aggcagatcc atctgttacc gtttcatcag
    29701 tacggcgaac cgaaataccg cctgctgggg aaaacatggt cgatgaaaga ggtgcctgcg
    29761 ccgtcgtcag ccgatgtgac aacgatgcgc gaaatggcag aacgggccgg atttcaggtt
    29821 accgtgggag gttaaaatgg catacctggt ggcagtaacc gcctgcgtaa gcggcgtggc
    29881 gcatacttat atggcggcgg aacggctgga aaagttgtgc cagttagaga agtggggcgt
    29941 cagcattgaa actcagggcg cgctgggaac ggagaatcgt ttagcggacg aggatatccg
    30001 tcgggcggat gttgctctgt tgattacgga tatcgagctt gccggtgccg agcgatttga
    30061 acattgccgc tatgtgcaat gcagcatcta cgcattcctg cgtgagccgc agcgggtaat
    30121 gagcgcggtg cgcaaagtgc tttctgcgcc gcagcaaacc catcttattc tggagtagtc
    30181 ggtttttctg tcagttggct gtggtactgc cggcgatatt ccgacggcga gtgttcggtg
    30241 tttttacgaa acagacggca gaagtagttg ctgtcgacaa agccgcaggc gtgcgccact
    30301 tcttttacct tcaggtcata gcctttcaat aacgtcttag cgtgctccag tcgcgtgtga
    30361 ttcaggtatt cgttaaaacc aatggccccc gttttttgaa acaggtgcga gagatagttt
    30421 ggtgaaatat agaatgcctg tgcgacagat tcgcgggtaa gcgcggaggc atagcgttcg
    30481 tcgatataat cgcgaatagc ttcaaatagt gcctggctgc gtgaggcggt ctggatttgg
    30541 ctgccgcgca aatcgcggca gtggctaagc aggctggcta cgataagcct tgccgtttgc
    30601 tgctcctgcg gctgcatttg catttcattg agcgtttgta gcagaaaaga acctatgcgt
    30661 gggccgcgtc gggcgacgtg ttgcttcgcc agattttgat attgtttgcc atcccattgc
    30721 acgacgctga agccgagttg ctgtttgcca aacagcacgc taaaggtggt agcgggtgtt
    30781 ttccattgtg ggaaattcca gccaccagcg gcaacgtaca gcacatcgcc gggaactaac
    30841 gttgctccag cgccggtatc aacaaactca ccttccagca caatttccag ccgtggaaaa
    30901 tcgacctgat acgcaagatc gggcaccggg ccgttactgc tggcaaagta aatctggcgc
    30961 agggacagcg ggccgttgat caggcgggag agcaaatagc tgacgtcgtg atacatgtta
    31021 ttcccttagg tatgtagacg ggaaaacagt aactggcatt attaaaaaag gccacctgaa
    31081 aagtgtggcc tgaggggagt tcgatgtaaa cattgcggca gtatcgcatc cggcaaatag
    31141 cgccagacgc tatacaaagg tcatctactg attacccacc tgatcgccat acggcagagt
    31201 gtcgtaatcg atcagcgcgt ttttcttgta cgggttacca atccagcggg tagtttcttt
    31261 gaactgcgga ttcaccactg aacgggttgg atcgtaaccg tcgtaagata agcccgccaa
    31321 atctgaccag gtgtggatca gttccgccag gctgtattta cgatcgacat cctgtgagaa
    31381 atcacgggga tgagtcgctt gccatttttc cgacgtccac agcaggaacg gaatggtgta
    31441 catgtggcgc gtcgggttgt cttcgttacg cccctgagtt ttgtgcggcg gcgtgtcgta
    31501 aacctcttca ccgtggtcag agaagtaaac caggaatccg ttcgggtctg ccgctttaaa
    31561 gtctttaatc aggctggcaa ccacatgatc gttatacagg ttagcgttgt cataatcgtt
    31621 atatgattcc agctcttctg cgcttaatcc tggaggaaca tgatcggtat tgccatcaaa
    31681 cttgccctga ttttccgggt agcggtattt gtatttgata tgcgtaccca gcagatgaac
    31741 gatgatcagt ttcttcggcg cagggtcatt cagcacatcc tggaacggct tcagcacgtt
    31801 ggtgtcgtat tcacgcgcac tctgcgtacg ttgctggttc atgtagtact gcttgtcggt
    31861 ctggcgcgaa aataccgtca gcatggtatt gcgggcggtc atcgtctgct ggttggtgat
    31921 ccagaaggtt ttataacccg cctgtttcat catgttcatc agcgacggct gcgtcagata
    31981 cagatccggg ttcttttcat tggcaaaggt cagcgcctgt tgcaggattt caatggtgta
    32041 cggacgagac gtaactacgt tattaaacac ggtcagattc ggatcggttt tatgcagcgc
    32101 atccagctcc ggcgtggttt cacgcggata accgtacaga ctcatgcgtc cgcgctgggt
    32161 cgactcgcca atcaccagca ctaaagtgcg cggttcgtta cccgattcat ctttgaaatt
    32221 agccagtggc ggcaaggcat tattttcatt cagtaacttt gttagcgagt ttagttgctg
    32281 acgatactga taatagccgg tcaggaattg ccacggtgcg gcaggctcca tacgcgaggc
    32341 taggttatcc aacgttttct caaacggctt gtttttgata aacgtattca tggcgatcgg
    32401 atgcagaatc aagccataaa gcagggcaaa agagacaaca taacgccacg gctttggaat
    32461 atagaccggg cgcaggcgtg tccacagcag aactgccacc gccgtatagg ccagcgcgat
    32521 aagcacaatt ttcaggctga aatactggct taaatactcg ctggcttcgt tggtgttggt
    32581 ttcgaacatc acaaacagaa cgctctgcga gaactcctgc ccgtagatga cgtagtagca
    32641 cagcgccgcc agagaggccg tccatagcac cacgccgatc actgcggcaa taattttaat
    32701 ccgcttcgga aagaggaata ccgggatcaa ccacagcgag ctgaataaca gcgagtcgcg
    32761 aatgccgttg gtgccactat aaccactgat gtaaataatg gcctgtagca gagtagagaa
    32821 aaaccaaaag tagagcagtg cccaacccag ggctttccag ctagaaaggg gtttagcctg
    32881 gacttctgtg gaatgcatag taagaacctg tcttgaaaaa aatatcgccg aatgtaacga
    32941 caattcctta aggatatctg aaggtatatt cagaatttga ataaaatgca gacagaaata
    33001 tattgaaaac gagggtgtta gaacagaagt atttcagaaa accctcgcgc aaaagcacga
    33061 gggtttgcag aagaggaaga ttagccggta ttacgcatac ctgccgcaat cccggcaata
    33121 gtgaccatta acgcttgttc gacgcgagga tccggttcct ggccttcttt ttctgcctgg
    33181 cgggagcggt gcagcaactc ggcctgcaat acgttcagcg ggtcggtgta aatattccgt
    33241 agctgaatag actctgcaat ccacggcaga tcggccatca gatgggaatc gttggcaatc
    33301 gccagcacca ctttgatgtc ttcttcttgc aggttgcgta actctttacc taacggccac
    33361 agtgctttgt ctaccagacg ttgatcatag tattccgcca accacaggtc tgctttggcg
    33421 aagaccatct ccagcatgcc gagacgcgtc gagaagaatg gccaatcgcg gcacatggct
    33481 tccagctcgc tctgtttgcc atcttcgacc actttttgca gcgccgtacc tgcacccagc
    33541 caggcgggga gcatcaggcg gttttgcgtc caggcaaaaa tccacggtat ggcgcgcagc
    33601 gactcgacgc cgccggttgg gcgacgtttc gccggacgtg aacccaacgg cagtttgccc
    33661 aattcttgtt ccggcgtagc ggagcggaag taaggcacaa aatctttgtt ttcacgtacg
    33721 tagccacggt agagatcgca ggagatgact gacagttcat ccataatgcg acgccagctc
    33781 tctttcggct caggtggtgg cagcaggttg gcttccagaa tcgccccggt gtaaagcgac
    33841 aggctgctga cggtgatttc tggcagacca tatttaaagc ggatcatctc gccctgttca
    33901 gtcacgcgca ggccaccttt caggcttcct ggcggttgtg acagcagcgc cgcatgggca
    33961 ggtgcgccgc cgcgaccaat ggaaccgccg cgaccgtgga acaacgtcag ctcaataccc
    34021 gctttttcgc aggttttgat taatgcatcc tgtgcctgat attgcgccca ggaagctgcc
    34081 atcacgcccg catcttttgc tgagtcggaa tagccaatca tcaccatctg tttgccctga
    34141 atcaggccgc gataccagtc gatattgagc agctgggtca tgacatcgtt ggcgttgttc
    34201 aggtcatcga gggtttcaaa cagcggagca accggcatcg caaacccgat acccgcttct
    34261 ttcagcagca ggtggacagc cagtacgtcg gacggcgttt tcgccatcga gatcacgtag
    34321 gcggcaatgg agccttgcgg cgcttcggca atcacctggc aggtatcgag cacttcgcgc
    34381 gtttcggcgc ttggttgcca gttgcgcggc agaagcggac gtttggagtt cagttcgcgg
    34441 atcaggaacg cctgtttgtc ggcctctgac cagctttcgt agtcgccgat accgaggtag
    34501 cgggtcagct cgcccagcgc ttcggtatgg cgggtgctct cctgacggat atcaatacgg
    34561 accagcggta cgccgaaaca tttcacgcgg cgcagggtgt cgagcagatc gccgttggcg
    34621 ataataccca tgccacacgc ctgaagtgac tggtagcaag cgtagagcgg ttcccacagt
    34681 tcttcgtttt gtgtcagcag cccttctggt tttggcagtt cttcgccttt caggcgcgct
    34741 tccagccatg cctgtgtcgc catcaggcga gaacgcaggt ttttcatcag atagcgatac
    34801 ggttctgcgg caccttcttc gccaaccagc gccagcagtt caggggtcgc ttcaaccatc
    34861 gacagttcag aaaccagaac ctgaatatct ttcaggaaca gatcggtggc tttccagcgg
    34921 ctgagcagca ggacgtggcg ggtgatatcg gcagtgacgt tcgggttgcc gtcgcggtca
    34981 ccgcccatcc acgaggtaaa acgtaccgga acaaattcga cgggcagttt gtagtcgaga
    35041 ttctcttcca gttgttcgtt cagttcgcgc aggtaattcg gtacgccttg ccacaggctg
    35101 ttttctacta cggcaaagcc ccatttggct tcatctaccg ggcttggacg cagcttacgg
    35161 atttcatcgg tatgccatga ctgggcgatc aactggtgca ggcgacgcat cagctggttg
    35221 tgttcgtagt cagcgatatc tttgttatcg agctgtttta aacaggcgtt cacttccacc
    35281 attttgtgga tcagtgtacg acgggtaatt tcggttgggt gagccgtgag gaccagttcc
    35341 agcgacagcg attccactgc ttttttgatg gtgtcttcgc tcagttccgg ctggtttttc
    35401 agtttacgca gggtgcgggc gatcacttcc gggttgctgg cagcttcgcc tttcggcgaa
    35461 atgctgtggt attgctcggc ggtgttggcc aggttcagga actgactaaa cgcacgcgca
    35521 acgggcagca gctcgtcgtt cgacaaattt tgtaaggtgg tgagcaactc ctggcggtta
    35581 gcatcattgc cagcgcgtga agatttcgac aacttacgga tagtttctac gcgttcaaga
    35641 atgtgttctc ccaacgcatc cttgatggtt tctcccagca ctttgccgag catactgaca
    35701 ttactacgca atgcggaata ttgttcgttc atattacccc agacacccca tcttatcgtt
    35761 tgatagccct gtatccttca cgtcgcattg gcgcgaatat gctcgggctt tgcttttcgt
    35821 cgtcttttat aaagccacgt aaaagcggtg acgtcaaatg ctgcgaaatc gcttcagcaa
    35881 acgaataaat agcaggaatt tacgccatta aattcacgac gctttaaata agcgtaactt
    35941 atggaaatgt taaaaaatcg ccatacataa caccaaaggt gtaggtcgga taagatgcgc
    36001 aagcatcgca tccgacatta ttgcggcacc ggagtttggc aacggtgccg gatgcggcgc
    36061 gagcgcctta tccggcctac gttttaatgc cagcaaaaat ggtgaattac ctggataatc
    36121 aactcacggg tgggtttaat aaaccgcgtc tccagatatt catcaggctg atgagcttga
    36181 ttgatggaac ccggaccgag caccagcgtc gggcataacg tttgaataaa cggcgcttcg
    36241 gtacagtaat tcaccacttc ggtttttgct ccgagcaatt tctcaaccac ttcaaccagt
    36301 tgatgattcg gtgggcattc atagccaggg atcggcggat gcagctcgtc gaccgtcaga
    36361 cgacccggcc agcgttcgct caccggagcc aatgcatcgt tgagcaaacc attaagttca
    36421 ttgagtgtca tgccaggctg cggacgaata tccatatgca actcacagca agcgcaaata
    36481 cggttagaag cgtcgccacc gtgaatatgc ccgaggttga gcgtagggta tggcacggta
    36541 aatgcttcgt agtgataacg ttctttcagg ttatcgcgca attgcaaaat atgcccgatg
    36601 gcgtcgtgca ttagttcgat agcgttaact ccgcgtgctg gatcgctgga gtgccccgac
    36661 tggccctgaa tacggatggc gttagagata tgacctttat gtgcgcgtac cggttgtagt
    36721 gacgtcggtt cgccaatgat ggcgcaatcc gggcgcaggg cggtagtttc ggcaaaataa
    36781 cgcgctccgg ccatactggt ttcttcatca gcagtcgcca gaatgtagag cggttttttc
    36841 agtttcgtga cgtcgacatc gcgtagcgca tcaaggataa acgcaaaaaa gcctttcatg
    36901 tcggcggtgc ctaagccgta aagcttgccg tcatgctccg tcagtgtaaa cggatcgcgc
    36961 gtccagcgac cgtcatcaaa tggcaccgta tcggtatgcc ccgccagcaa caagccgcca
    37021 gccccctgtc cgtaactggc cagcatatta aatttgttgc gagttcctgg aacaggctgc
    37081 acttccacat tgaagcccaa atctttaaac cagtccgcca gcagagtgat taaatctgca
    37141 ttgctttgat cgagtgcctc ttccgtagcg cttattgaag gtgtggcaat cagagcgcgg
    37201 taaatctcga taaatggcgg taatttgttt ttcattgttg acacacctct ggtcatgata
    37261 gtatcaatat tcatgcagta tttatgaata aaaatacact aacgttgagc gtaataaaac
    37321 ccaccagccg taaggtgaat gttttacgtt taacctggca accagacata agaaggtgaa
    37381 tagccccgat gttgaatacg ctgattgtgg gtgccagcgg ctacgctggc gcagagctag
    37441 tgacctatgt aaatcgccat ccgcatatga acataaccgc tttgactgtt tcagcgcaaa
    37501 gcaatgatgc gggaaagtta atctccgatt tgcatccgca gctaaaaggc atcgttgagc
    37561 tgccgttgca gccgatgtcg gatatcagcg agtttagccc aggggtggac gtagtgtttc
    37621 tcgccaccgc ccatgaagtt agccacgatt tagcgccgca gtttcttgaa gcgggctgcg
    37681 tggtgttcga cctttccggc gcgtttcgtg ttaacgacgc caccttctat gaaaaatatt
    37741 acggctttac ccatcaatac ccggaactgc tggaacaggc agcctacggt ctggcggagt
    37801 ggtgcggtaa taaattaaaa gaagcgaatt tgattgcggt gccaggctgt tatccgacgg
    37861 cggcacagct ggcgctgaaa ccgttgattg atgccgatct tcttgacctc aatcagtggc
    37921 cggtgatcaa cgccaccagc ggcgtgagcg gtgcaggacg taaggcagcg atttcaaata
    37981 gtttttgtga agttagcctg caaccgtatg gcgtctttac tcatcgccat caaccagaga
    38041 tcgccacaca cctcggtgct gacgttatct tcaccccaca tctgggcaat ttcccgcgcg
    38101 gcattctcga aaccattacc tgccgcctga aatcgggtgt gacccaggcg caagtcgcgc
    38161 aagcgttaca gcaggcgtat gcccataaac cgctggtgcg gctgtatgac aaaggcgttc
    38221 cggcgctgaa aaatgtcgtt gggctgccat tttgcgatat cgggtttgcc gttcagggcg
    38281 agcatctgat tattgtggcg accgaagaca acttactgaa aggcgcggcg gcacaagcgg
    38341 tacagtgcgc caatattcgt ttcggctatg cggaaacgca gtctcttatt taagggtgca
    38401 atgatgaatc cattaattat caaactgggc ggcgtactgc tggatagtga agaggcgctg
    38461 gaacgtctgt ttagcgcatt ggtgaattat cgtgagtcac atcagcgtcc gctggtgatt
    38521 gtgcacggcg gcggttgcgt ggtggatgag ctgatgaaag ggctgaatct gccggtgaaa
    38581 aagaaaaacg gcctgcgggt gacgcctgct gatcagatag acattatcac cggagcactg
    38641 gcgggaacgg caaataaaac cctgttggca tgggcgaaga aacatcagat tgcggccgta
    38701 ggtttgtttc tcggtgacgg cgacagcgtc aaagtgaccc agcttgatga agagttaggt
    38761 catgttggac tggcgcagcc aggttcgcct aagcttatca actccttgct ggagaacggt
    38821 tatctgccgg tggtcagctc cattggcgta acagacgaag ggcaactgat gaacgtcaat
    38881 gccgaccagg cggcaacggc gctggcggca acgctgggcg cggatctgat tttgctctcc
    38941 gacgtcagcg gcattctcga cggcaaaggg caacgcattg ccgaaatgac cgccgcgaaa
    39001 gcagaacaac tgattgagca gggcattatt actgacggca tgatagtgaa agtgaacgcg
    39061 gcgctggatg cggcccgcac gctgggccgt ccggtagata tcgcttcttg gcgttatgcg
    39121 gagcagcttc cggcactgtt taacggtatg ccgatgggta cgcggatttt agcttaagtt
    39181 ttgttagccg gaggagcagc tttccggcat tgaatttcaa aataaggaaa cagagttatg
    39241 gcactttggg gcgggcgttt tacccaggca gcagatcaac ggttcaaaca attcaacgac
    39301 tcactgcgct ttgattaccg tctggcggag caggatattg ttggctctgt ggcctggtcc
    39361 aaagccttgg taacggtcgg cgtgttaacc gcagaagagc aggcgcaact ggaagaggcg
    39421 ctgaacgtgt tgctggaaga tgttcgcgcc aggccacaac aaatccttga aagcgacgcc
    39481 gaagatatcc atagctgggt ggaaggcaaa ctgatcgaca aagtgggcca gttaggcaaa
    39541 aagctgcata ccgggcgtag ccgtaatgat caggtagcga ctgacctgaa actgtggtgc
    39601 aaagataccg ttagcgagtt actgacggct aaccggcagc tgcaatcggc gctggtggaa
    39661 accgcacaaa acaatcagga cgcggtaatg ccaggttaca ctcacctgca acgcgcccag
    39721 ccggtgacgt tcgcgcactg gtgcctggcc tatgttgaga tgctggcgcg tgatgaaagc
    39781 cgtttgcagg atgcgcttaa gcgtctggat gtcagcccat taggctgtgg cgcgctggcg
    39841 ggaacggcct atgaaatcga ccgtgaacag ttagcaggct ggctgggctt tgcttcggcg
    39901 acccgtaaca gtctggacag tgtttctgac cgtgaccatg tgttggaact gctttctgct
    39961 gccgctatcg gcatggtgca tctgtcgcgt tttgctgaag atctgatttt ctttaacacc
    40021 ggcgaagcgg ggtttgtgga gctttcggac cgcgtgactt ccggttcatc attaatgccg
    40081 cagaagaaaa acccggatgc gctggagctg attcgcggta aatgcggccg ggtgcagggt
    40141 gcgttaaccg gcatgatgat gacgctgaaa ggtttgccgc tggcttacaa caaagatatg
    40201 caggaagaca aagaaggtct gttcgacgcg ctcgatacct ggctggactg cctgcatatg
    40261 gcggcgctgg tactggacgg cattcaggtg aaacgtccgc gttgtcagga agcggcgcag
    40321 caggggtatg cgaacgccac tgaactggcg gattacctgg tggcgaaagg cgtacctttc
    40381 cgcgaggcgc accatattgt gggtgaagcg gtggtggaag ccattcgtca gggcaaaccg
    40441 ctggaagatc tgccactcag tgagttgcag aaattcagtc aggtgattgg cgaagatgtc
    40501 tatccgattc tgtcgctaca atcgtgcctc gaaaagcgtg cggcaaaagg cggcgtctca
    40561 ccgcagcagg tggcgcaggc gattgctttt gcgcaggctc ggttagagta atttgtttat
    40621 attgctcccc tgaaaatggg gagtttacca cgcctacaaa gtgcgacact tcgtaggcgg
    40681 ataaggcgtt tacgccgcat caggcagaaa tttgtca
//
LOCUS       DAJVQH010000018        38754 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJVQH010000018 DAJVQH010000000
VERSION     DAJVQH010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.018"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="OQM87_001104"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="OQM87_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCS1507326.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..579
                     /gene="hisL"
                     /locus_tag="OQM87_001105"
     CDS             529..579
                     /gene="hisL"
                     /locus_tag="OQM87_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCS1507327.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    555..678
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            725..1624
                     /gene="hisG"
                     /locus_tag="OQM87_001106"
     CDS             725..1624
                     /gene="hisG"
                     /locus_tag="OQM87_001106"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCS1507328.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1630..2934
                     /gene="hisD"
                     /locus_tag="OQM87_001107"
     CDS             1630..2934
                     /gene="hisD"
                     /locus_tag="OQM87_001107"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCS1507329.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2931..4001
                     /gene="hisC"
                     /locus_tag="OQM87_001108"
     CDS             2931..4001
                     /gene="hisC"
                     /locus_tag="OQM87_001108"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCS1507330.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            4001..5068
                     /gene="hisB"
                     /locus_tag="OQM87_001109"
     CDS             4001..5068
                     /gene="hisB"
                     /locus_tag="OQM87_001109"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCS1507331.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5068..5658
                     /gene="hisH"
                     /locus_tag="OQM87_001110"
     CDS             5068..5658
                     /gene="hisH"
                     /locus_tag="OQM87_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCS1507332.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5658..6395
                     /gene="hisA"
                     /locus_tag="OQM87_001111"
     CDS             5658..6395
                     /gene="hisA"
                     /locus_tag="OQM87_001111"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCS1507333.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6377..7153
                     /gene="hisF"
                     /locus_tag="OQM87_001112"
     CDS             6377..7153
                     /gene="hisF"
                     /locus_tag="OQM87_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCS1507334.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7147..7758
                     /locus_tag="OQM87_001113"
     CDS             7147..7758
                     /locus_tag="OQM87_001113"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCS1507335.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7944..8921)
                     /gene="wzzB"
                     /locus_tag="OQM87_001114"
     CDS             complement(7944..8921)
                     /gene="wzzB"
                     /locus_tag="OQM87_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCS1507336.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9067..10233)
                     /gene="ugd"
                     /locus_tag="OQM87_001115"
     CDS             complement(9067..10233)
                     /gene="ugd"
                     /locus_tag="OQM87_001115"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCS1507337.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEDYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10483..11889)
                     /gene="gndA"
                     /locus_tag="OQM87_001116"
     CDS             complement(10483..11889)
                     /gene="gndA"
                     /locus_tag="OQM87_001116"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCS1507338.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11998..12357)
                     /locus_tag="OQM87_001117"
     CDS             complement(11998..12357)
                     /locus_tag="OQM87_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507339.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(13007..13570)
                     /locus_tag="OQM87_001118"
     CDS             complement(13007..13570)
                     /locus_tag="OQM87_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507340.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13778..14716)
                     /locus_tag="OQM87_001119"
                     /pseudo
     CDS             complement(13778..14716)
                     /locus_tag="OQM87_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14779..15153)
                     /locus_tag="OQM87_001120"
     CDS             complement(14779..15153)
                     /locus_tag="OQM87_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCS1507341.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFELLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15157..16305)
                     /gene="wzy"
                     /locus_tag="OQM87_001121"
     CDS             complement(15157..16305)
                     /gene="wzy"
                     /locus_tag="OQM87_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCS1507342.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16325..17230)
                     /gene="rfbG"
                     /locus_tag="OQM87_001122"
     CDS             complement(16325..17230)
                     /gene="rfbG"
                     /locus_tag="OQM87_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCS1507343.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17223..18113)
                     /locus_tag="OQM87_001123"
     CDS             complement(17223..18113)
                     /locus_tag="OQM87_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCS1507344.1"
                     /translation="MNSNIYAVIVTYNPELKNLNELITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18143..19399)
                     /gene="wzx"
                     /locus_tag="OQM87_001124"
     CDS             complement(18143..19399)
                     /gene="wzx"
                     /locus_tag="OQM87_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCS1507345.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19415..19960)
                     /gene="rfbC"
                     /locus_tag="OQM87_001125"
     CDS             complement(19415..19960)
                     /gene="rfbC"
                     /locus_tag="OQM87_001125"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCS1507346.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19965..20843)
                     /gene="rfbA"
                     /locus_tag="OQM87_001126"
     CDS             complement(19965..20843)
                     /gene="rfbA"
                     /locus_tag="OQM87_001126"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1507347.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20902..21801)
                     /gene="rfbD"
                     /locus_tag="OQM87_001127"
     CDS             complement(20902..21801)
                     /gene="rfbD"
                     /locus_tag="OQM87_001127"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCS1507348.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21801..22886)
                     /gene="rfbB"
                     /locus_tag="OQM87_001128"
     CDS             complement(21801..22886)
                     /gene="rfbB"
                     /locus_tag="OQM87_001128"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1507349.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23259..24152)
                     /gene="galF"
                     /locus_tag="OQM87_001129"
     CDS             complement(23259..24152)
                     /gene="galF"
                     /locus_tag="OQM87_001129"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCS1507350.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24327..25721)
                     /gene="wcaM"
                     /locus_tag="OQM87_001130"
     CDS             complement(24327..25721)
                     /gene="wcaM"
                     /locus_tag="OQM87_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCS1507351.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25732..26952)
                     /gene="wcaL"
                     /locus_tag="OQM87_001131"
     CDS             complement(25732..26952)
                     /gene="wcaL"
                     /locus_tag="OQM87_001131"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCS1507352.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSYEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26949..28229)
                     /gene="wcaK"
                     /locus_tag="OQM87_001132"
     CDS             complement(26949..28229)
                     /gene="wcaK"
                     /locus_tag="OQM87_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCS1507353.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRSYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMIADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28409..29887)
                     /gene="wzxC"
                     /locus_tag="OQM87_001133"
     CDS             complement(28409..29887)
                     /gene="wzxC"
                     /locus_tag="OQM87_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCS1507354.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVSVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29889..31283)
                     /gene="wcaJ"
                     /locus_tag="OQM87_001134"
     CDS             complement(29889..31283)
                     /gene="wcaJ"
                     /locus_tag="OQM87_001134"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCS1507355.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDTKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31338..32708)
                     /gene="cpsG"
                     /locus_tag="OQM87_001135"
                     /gene_synonym="manB"
     CDS             complement(31338..32708)
                     /gene="cpsG"
                     /locus_tag="OQM87_001135"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCS1507356.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32813..34249)
                     /gene="cpsB"
                     /locus_tag="OQM87_001136"
     CDS             complement(32813..34249)
                     /gene="cpsB"
                     /locus_tag="OQM87_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCS1507357.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASSEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGHHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34252..35475)
                     /gene="wcaI"
                     /locus_tag="OQM87_001137"
     CDS             complement(34252..35475)
                     /gene="wcaI"
                     /locus_tag="OQM87_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCS1507358.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLLFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35472..35951)
                     /gene="gmm"
                     /locus_tag="OQM87_001138"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35472..35951)
                     /gene="gmm"
                     /locus_tag="OQM87_001138"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCS1507359.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35951..36919)
                     /gene="fcl"
                     /locus_tag="OQM87_001139"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35951..36919)
                     /gene="fcl"
                     /locus_tag="OQM87_001139"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCS1507360.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36922..38043)
                     /gene="gmd"
                     /locus_tag="OQM87_001140"
     CDS             complement(36922..38043)
                     /gene="gmd"
                     /locus_tag="OQM87_001140"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCS1507361.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38069..38617)
                     /gene="wcaF"
                     /locus_tag="OQM87_001141"
     CDS             complement(38069..38617)
                     /gene="wcaF"
                     /locus_tag="OQM87_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCS1507362.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38633..>38754)
                     /locus_tag="OQM87_001142"
     CDS             complement(38633..>38754)
                     /locus_tag="OQM87_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCS1507363.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10349 a         9688 c         8721 g         9996 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgagt tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcaccatcat cctgactagt ctttcaggcg atgtgtgctg
      601 gaagacattc agatcttcca gtggtgcatg aacgcatgag aaagcccccg gaagatcacc
      661 ttccgggggc ttttttattg cgcggttgtt aacggttcag acaggtttaa agaggaataa
      721 gaaaatgaca gacaacactc gtttacgcat agctatgcag aaatccggcc gtttaagtga
      781 tgactcacgc gaattactgg cgcgctgtgg cattaaaatc aatcttcaca cccagcgcct
      841 gatcgcgatg gcagaaaaca tgccgattga tattctgcgc gtgcgtgacg atgacattcc
      901 cggtctggta atggacggcg tggtagacct tgggattatc ggcgaaaacg tgctggaaga
      961 agagctgctt aaccgccgcg cccagggtga agatccgcgc tactttaccc tgcgtcgtct
     1021 ggatttcggc ggctgccgcc tttcgctggc aacgccggtt gatgaagcct gggacggtcc
     1081 gctctcctta aacggtaaac gtatcgccac ctcttatcct cacctgctca agcgttatct
     1141 cgaccagaaa ggtatctctt ttaaatcctg cttactgaac ggttctgtcg aagttgcgcc
     1201 gcgtgccggg ctggcggatg cgatttgcga tctggtttcc accggtgcca cgctggaagc
     1261 taacggcctg cgcgaagtcg aagttatcta ccgctcgaaa gcctgcctga tccagcgcga
     1321 tggcgaaatg gaagaatcta aacagcaact gatcgacaag ctactgaccc gtattcaggg
     1381 tgtgatccag gcgcgcgaat caaaatacat catgatgcac gcaccgaccg aacgcctaga
     1441 tgaagtcatc gccctgctgc caggtgccga acgcccaacc attctgccgc tggcgggtga
     1501 tcaacagcgc gtagcgatgc acatggtgag tagcgaaacc ctgttctggg aaacgatgga
     1561 aaaactgaaa gcactgggtg ccagttcaat cctggtcctg ccgattgaga agatgatgga
     1621 gtgatcgcca tgagctttaa cacaatcatt gactggaata gctgtactgc ggagcaacaa
     1681 cgccagctgt taatgcgccc ggcgatctcc gcttctgaaa gcattacccg caccgttaac
     1741 gatattctcg ataacgtgaa aacgcgtggc gatgaggccc tgcgtgaata cagcgcgaag
     1801 tttgataaaa ccacggttac cgcgctgaag gtgtctgctg atgagatcgc cgccgccagc
     1861 gaacgcctga gcgacgagct aaaacaggcg atggcggtgg cagtaaagag tattgaaacc
     1921 ttccacactg cgcaaaaact gccgccggta gatgtagaaa cgcagccagg cgtgcgttgc
     1981 cagcaggtca cgcgcccggt ggcttcagtg ggtttgtata ttccgggcgg ctccgccccg
     2041 ctcttctcaa cggtattaat gctggcaact ccggcgcgta ttgcgggctg taaaaaagtg
     2101 gtgctgtgct caccgccgcc gattgccgat gaaattcttt atgcggcgca gctgtgcgat
     2161 gtgcaggacg tgtttaacgt cggcggcgca caggccattg ccgcgctggc gtttggtacg
     2221 gaatctgtgc cgaaagtgga caaaatcttc gggccgggta acgcctttgt caccgaagca
     2281 aaacgccagg taagccagcg tctggacggt gcggcgatcg atatgcccgc aggcccgtcg
     2341 gaagtgctgg tgattgctga cagcggcgct acgccggatt ttgtggcttc tgatttgctt
     2401 tctcaggctg aacacggccc tgactcacag gtaattttac tgacccccga tgctgatatg
     2461 gcgcgtcgcg ttgccgaggc cgtcgaacgc caactggcag aactgccgcg tgccgaaacc
     2521 acccgccagg cactgaacgc cagccgcctg atcgtgacta aagatttagc gcagtgcgtg
     2581 gagatctcca accagtacgg cccggagcac ctgatcattc agacccgcaa cgcccgcgaa
     2641 ctggtcgatg gcatcaccag cgccggttcg gtatttcttg gtgactggtc accggaatcg
     2701 gcaggcgact acgcctccgg caccaaccac gtgctgccga cttacggtta caccgccacc
     2761 tgttccagcc tcgggctggc agatttccag aaacgcatga ccgtacagga actgtcgaaa
     2821 gaaggtttct ccgcgctggc ttcgaccatt gaaacactgg ccgccgccga gcgcctgacc
     2881 gcccacaaaa atgccgttac tttgcgtgtt aacgtcctta aggagcaagc atgagcaccg
     2941 tgactattac tgatttagcg cgtgaaaacg tccgcaacct gacgccgtat cagtcggcgc
     3001 gtcgtctggg cggtaacggc gacgtctggc tgaacgccaa cgaatatccc acagccgtgg
     3061 agtttaagct tactcagcaa acgctcaacc gctacccgga atgccagcca aaagcggtga
     3121 ttgaaaatta cgcgcaatat gcaggcgtaa aaccggaaca ggtgctggtc agccgtggtg
     3181 cggacgaagg tattgaactg ctgatccgcg ctttttgcga accgggtaaa gacgccatcc
     3241 tctactgccc gccaacgtac ggcatgtaca gcgtcagcgc tgaaaccatt ggtgtcgaat
     3301 gccgcacagt gccgacgctg gaaaactggc aactggactt acagggcatt tccgacaagc
     3361 tggacggcgt aaaagtggtc tatgtttgca gccccaacaa ccccaccggg caactgatca
     3421 atccgcagga ttttcgcacc ctgctggagc taacccgcgg taaggcgatt gtggttgccg
     3481 atgaagctta tatcgagttt tgcccgcagg catcgttagc tggctggctg gcggaatatc
     3541 cgcacctggc gattttgcgt accctttcga aagccttcgc gttggcgggg cttcgttgcg
     3601 gatttacgct ggcaaacgaa gaagtcatca acctgctgat gaaagtgatc cccccctacc
     3661 cgctctcgac gccggttgcc gacattgccg cacaggcgtt aagcccgcag ggaatcgtcg
     3721 ccatgcgcga acgggtggcg caaattatta cagaacgcga atacctgatt gccgcactga
     3781 aagagatccc ctgcgtggag caggttttcg actctgaaac caactacatt ctggcgcgct
     3841 ttaaagcctc cagtgcagtg tttaaatctt tgtgggatca gggcattatc ttacgtgatc
     3901 agaataaaca accctcttta agcggctgcc tgcgaattac cgtcggaacc cgtgaagaaa
     3961 gccagcgcgt cattgacgcc ttacgtgcgg agcaagtttg atgagtcaga agtatctttt
     4021 tatcgatcgc gatggaaccc tgattagcga accgccgagt gattttcagg tggaccgttt
     4081 tgacaaactc gcctttgaac cgggcgtgat cccggaattg ctgaagctgc aaaaagaggg
     4141 ctacaagctg gtgatgatca ctaatcagga tggtctggga acacaaagtt tcccgcaagc
     4201 ggattttgat ggcccgcaca acctgatgat gcagatcttc acctcgcaag gcgtgcagtt
     4261 tgatgaagtg ctgatttgtc cgcacctgcc cgccgatgag tgcgactgcc gtaagccgaa
     4321 agtaaaactg gtggagggtt atctggctga gcaagcgatg gatcgtgcca acagttatgt
     4381 aattggcgat cgcgcgaccg acattcaact ggcggaaaac atgggcatta atggtttacg
     4441 ctacgaccgc gaaaccctga actggccaat gattggcgag caactcacta aacgagaccg
     4501 ttacgcccat gtagtgcgca acaccaaaga gacgcagatt gacgtccagg tgtggctgga
     4561 tcgcgaaggt ggcagcaaga ttaacaccgg cgttggcttc tttgatcaca tgctggatca
     4621 gatcgccacc cacggcggtt tccgcatgga aatcaatgtc aaaggcgacc tctatatcga
     4681 cgatcaccac accgtcgaag ataccggcct ggcgctgggc gaagcgttaa aaattgccct
     4741 cggcgacaaa cgcggtattt gccgctttgg ttttgtgctg ccgatggacg aatgccttgc
     4801 ccgctgcgca ctggatatct ctggtcgccc gcacctggaa tataaagccg agtttaccta
     4861 ccagcgtgtg ggcgatctca gcactgaaat gatcgaacac ttcttccgtt cgctctctta
     4921 caccatgggc gtgacactac acctgaaaac caaaggtaaa aacgatcatc accgtgtaga
     4981 aagcctgttc aaagcctttg gtcgtaccct gcgccaggcc atccgcgtcg aaggcgacac
     5041 cctgccctcg tcgaaaggag tgctgtaatg aacgtggtga tccttgatac tggctgcgcc
     5101 aacctgaact ctgtgaagtc tgccattgcg cgccacggtt atgaacccaa agtcagccgt
     5161 gacccggaca ttgtgttgct ggccgataaa ctgtttttac ccggcgtcgg caccgcacaa
     5221 gcagcgatgg atcaggtgcg tgagcgcgag ctgtttgatc tcatcaaagc ctgtacccaa
     5281 ccggtgctgg gtatctgctt agggatgcaa ctcctgggac gacgcagcga agagagcaat
     5341 ggcgtcgact tgctgggcat catcgacgaa gacgtgccga aaatgaccga ctttggtctg
     5401 ccactgccac atatgggctg gaaccgcgtt tatccgcagg caggcaaccg cctgtttcag
     5461 gggattgaag acggcgcgta cttttacttt gttcacagct acgcaatgcc agtcaatccg
     5521 tggaccatcg cccagtgtaa ttacggcgaa ccgttcaccg cggcggtaca aaaagataac
     5581 ttctacggcg tgcagttcca cccggagcgt tctggtgccg ctggcgctaa gttgctgaaa
     5641 aacttcctgg agatgtgatg attattccgg cattagattt aatcgacggc accgtggtgc
     5701 gtctccatca gggcgattac ggcaaacaac gcgattacgg taacgacccg ctgccgcgct
     5761 tacaggatta cgctgcgcaa ggtgccgaag tgttgcacct ggtggatctg accggggcaa
     5821 aagatccggc taagcgtcaa atcccgctga ttaaaaccct ggtcgcgggc gttaacgtcc
     5881 cggtgcaggt tggcggcggt gttcgtagtg aagaagacgt ggcggcgtta ctggaagctg
     5941 gcgttgcgcg cgtggtggtc ggttccaccg cagtgaaatc accggacgta gtgaaaggct
     6001 ggtttgaacg tttcggtgcc gatgcgctgg tgctggcact ggatgtccgt attgacgagc
     6061 aaggcaacaa gcaagtggca gtcagcggct ggcaagagaa ctcgggcatt tcactggaac
     6121 aactggtgga aacctatctg cccgtcggcc tgaaacatgt gttgtgtacc gatatctcgc
     6181 gcgacggcac gctggcggga tctaacgtct ctttatatga agaagtgtgt gccagatatc
     6241 cgcaagtggc gtttcagtcc tccggcggca ttggcgacat taacgatgtt gctgccctac
     6301 gtggcactgg cgtacgcggc gtaatagttg gtcgtgcatt actggaaggt aaattcaccg
     6361 tgaaggaggc catcgcatgc tggcaaaacg cataatcccg tgtctcgacg ttcgtgatgg
     6421 tcaggtggtg aaaggcgtac agtttcgcaa ccatgaaatc attggcgata tcgtgccact
     6481 ggcaaaacgc tacgccgaag aaggtgcaga tgaactggtg ttctacgata tcaccgcttc
     6541 cagcgatggc cgtgtggtag ataaaagctg ggtatctcgc gtggcggaag tgatcgacat
     6601 tccgttttgt gtggcgggtg ggattaagtc tctggaagat gccgcgaaaa ttctttcctt
     6661 tggcgcggat aaaatttcca tcaactcccc tgcgctggca gacccaacat taattactcg
     6721 cctggccgat cgctttggcg tgcagtgtat tgtggttggt attgatacct ggtacgacgc
     6781 cgaaaccggt aaatatcatg taaatcaata taccggcgat gaaagccgca cccgcgtcac
     6841 tcaatgggaa acgctcgact gggtagagga ggtgcaaaaa cgcggcgccg gagaaatcgt
     6901 cctcaacatg atgaatcagg acggcgtgtg taacggttac gacctcaagc agctgaaaaa
     6961 agtgcgtgaa gtttgccacg tcccgctgat tgcctccggt ggcgcgggca ccatggaaca
     7021 cttcctggaa gccttccgcg atgccgacgt tgacggtgcg ctggcagctt ccgtattcca
     7081 caaacaaata atcaatattg gcgaattaaa agcgtacctg gcaacacagg gcgtggagat
     7141 caggatatgt taacagaaca acaacgtcgc gaactggact gggaaaaaac cgacggtctg
     7201 atgccggtga ttgtgcaaca cgcggtatct ggcgaagtgt taatgctggg ctacatgaac
     7261 ccggaagcct tagacaaaac catcgaaagc ggtaaagtca ccttcttctc gcgcactaaa
     7321 cagcgactgt ggaccaaagg cgaaacctcg ggcaattttc tcaatgtggt aagtattgcc
     7381 ccggactgcg acaacgacac attactggtg ctggcgaatc ccatcgggcc aacctgccac
     7441 aaaggcacca gtagctgctt cggcaacacc gctcaccagt ggctgttcct gtatcaactg
     7501 gaacaactgc tcgccgaacg taaatatgcc gacccggaaa cctcctacac cgccaaactg
     7561 tatgccagcg gcactaaacg cattgcgcag aaagtgggcg aagaaggcgt ggaaaccgca
     7621 ctggcagcaa cggtacatga tcgctttgag ctgaccaacg aggcatctga cttgatgtat
     7681 cacctgctgg tgctgttgca ggatcaggat ctggatttaa cgacagtaat tgagaacctg
     7741 cataaacggc atcagtgagt tacgggctga gcaggtgtga tgttgttgtc acacccggag
     7801 ccattgcccg atgcgcaaac ttatcgtgcc tacacattat ctcaacacac gtaggccgga
     7861 taagatgcgc cagcatcgca tccggcaaaa aaaacgggca tggtgtcacc accctgcccg
     7921 tttctcttaa atgcacaata atattacttc gcgttgtaat tacgcagagc gttacgcccc
     7981 aacacaatcc ccgcgccaac catgccgccc agaagcactg ccagaatcaa ggtgattgcc
     8041 tttttcggac tatcgcgacg aataggtaac gtcggtttca tcacatagcg gtaagcatga
     8101 atatcaagat catcaaactt taatttttca atatccaaca ggttttgacg tgtctgatag
     8161 tagtttggtg agaacaccaa cggacgagtc gcttcatgct taatcatcga ctccagcgct
     8221 tcgctcccta gaaggaacaa cgtatcttgc gtcacatctt cagtctgctg aacctgtggc
     8281 tttgtcacct gcgcctgatt cgcatactgc aacgcttcct gaatctgacg gatacgcaga
     8341 tctttctgct cctgcgcgac cacttcctgg gttcttaaag agtcctgcaa gtttttccgt
     8401 cccagagcaa tgttgtcctt gagatccttt tctagctctt gattcacttt atcatcaact
     8461 tgctgaatgt attgggccaa cttcatttgt gcgccctctg cagtttgccc aacataagaa
     8521 acagtcaatg gtaattgttg gttcttaaca gaaggttcga tggtaagttt ttctggctct
     8581 tcctgattat ccagcgtttc tgctaatgca gagaaggcag aactgaagcg accaattaac
     8641 gtctcctgca aatccgatac tttcggtgca gcctgaccat agataacatt catggcattg
     8701 ttatagccag caatttgccc cacgtcgggc tgagtgataa ttgctgttga cgtccatttc
     8761 tccttcgcta ctgccaaata accaatagcc agggcaatag ccacaatgac ggaaatgata
     8821 attgtcatct tgccacgcca caactgcact agtaaatcaa tcaaatcaat ctgttccggg
     8881 tcatggtttt gcccagaaac attattattt tctactctca tcattaccct aactgacgaa
     8941 aatttccaag tccgcatagt ttagcgataa tccccgtgaa tgctatagga taagtgataa
     9001 aaataatgaa ttattgcgga ggtaacaaaa gacaatcagg gcgtaaatag ccctgataac
     9061 aagatgttag tcgctgccaa agagatcgcg ggtgtagacc ttatctgcca catccttaag
     9121 ctcttctgcc atacggttgg agataatgac gtcggcttgt tgtttgaagg tggcgagatc
     9181 acgttccagg cgagagttga agaatgagtc ttctttcatc accggctcgt agatgatcac
     9241 ttcaacgcct ttcgccttga tacgcttcat aatcccctga atagaagacg tacggaagtt
     9301 atctgacccg ctcttcataa tcagacgata aatacccacc acttgcggtt tgcgtgacaa
     9361 aatggcatcg gcaataaaat ctttacgcgt gcggtttgca tcgacaattg ccgagatcag
     9421 gttattcggc acagactggt agttcgccag taactgcttg gtatctttcg gcagacaata
     9481 accaccataa ccaaacgacg gattgttgta atggttgcca atacgcggat cgagacaaac
     9541 gccttcgata atctggcgag tattcagacc taaactttct gcataactat ccagttcatt
     9601 aaagtatgct acgcgcatcg ccagataggt attagcgaaa agtttaatcg cttctgcttc
     9661 agtggagtcg gtaaacaggg tcgggatatt ttgcttaatc gcgccttcct gtaacaacgc
     9721 agcaaaacgt tctgcgcgtt ctgaacgctc accgatgaca atacgtgacg gatggagatt
     9781 atcgtaaagg gctttacctt cgcggaggaa ttccggagag aaaataatat tttcagtacg
     9841 atatttctta tgcatcgctg cggtaaaacc aacgggaacc gttgatttga tgaccataac
     9901 cgcataagga tttatctcaa ctacgtcttt aattactgat tctacactgg atgtattgaa
     9961 ataattagtt ttaggatcat agtcggttgg agtggcgatg atgacataat cagcatcccg
    10021 gtagtcttca tttttatcta atgtggcatt aaagtgtatt ttatctgatt gcaaaaactg
    10081 ctgaatttcc ttatcaacaa taggagatat ccgatcattc agcatagcaa cgcgtgacgg
    10141 taaaatatct aatgccacaa cctcatgatt ttgtgcgatt agaagcccgt ttgacaagcc
    10201 tacatagcca gtaccggaaa tggtgatttt catttcatgc tctcagaatt aacttaactg
    10261 tgaatcatga tgtttttagc atcgtgataa gatgctaaaa gttttaacgc tacgttgtca
    10321 gatggtcaac gcaacgaaaa acaattcaga ttttctttat aagaatagct gatgtttatt
    10381 aagttaatat taagcagtga aatttagtct ataagatatt tggcaaaaaa aagcccggtg
    10441 caatatacgc cgggcctcaa ttttattgtt ggttaaatca gattaatcca gccattcagt
    10501 atggaacaca ccttctttat caatgcgctt ataagtatgc gccccgaaat agtcacgctg
    10561 tgcctggatc aggttcgcag gcagaaccgc ggcacggtag ctatcgtaat aggcaaccgc
    10621 agcggcgaag gtcggaaccg ggataccgtt ctgtactgca taagcgacga catcgcgcag
    10681 cgcctgctgg tagtcatcgg caatttgctt gaagtacggt gccagcagca ggttagcgat
    10741 ctgcggattt tcggcataag catcggtgat tttctgcagg aactgcgcac ggatgatgca
    10801 gccagcacgg aaaatcttcg cgatttcgcc gtagttcaga tcccagttgt attcttcaga
    10861 cgcagcacgc agctgagaga agccctgagc gtaagaaacg attttgccca gatacagcgc
    10921 actgcgaact ttttcgatga actcagcctt gtcgcctgct gactgagctt gcggaccaga
    10981 gagaacttta gatgcggcaa cgcgctgatc tttcagagaa gagatataac gtgcaaacac
    11041 agactcggta atcagcgaca gcggttcgcc gagatccagc gcgctctggc tggtccattt
    11101 accggtgcct ttgtttgctg cttcatccag aatcacatca accaggtagt taccgtcttc
    11161 atcttttttg gtgaagatat ctttagtaat gtcgatcagg tagctgctaa gttcaccgtt
    11221 attccactcg gtgaaagtct gggccagttc ttcattagat agattcaggc cacctttgag
    11281 cagagaatac gcttcagcaa tgagctgcat atctccgtat tcaataccat tgtgaaccat
    11341 cttcacataa tggcctgcgc cgtcggcacc aatataagta acgcatggct cgccgtcttc
    11401 tgccacggca gcgattttag tcaggatagg cgcaaccagc tcataagcct ctttctgccc
    11461 gccaggcata atagaaggac ctttcagcgc gccctcttca ccgccggaaa caccggtacc
    11521 gatgaagtta aagccctctg ctgaaagctc acgattacga cgaatggtgt cctggaagaa
    11581 ggtattacca ccatcaatga tgatgtcacc tttatcgagg tatggtttca gggaatcaat
    11641 cgcagcatcc gtgcctgcac ctgctttcac cattaacagg atgcgacgag gcgtttccag
    11701 agattcaaca aactctttca ccgtatagta aggagccagt ttcttgcctg gattttcagc
    11761 aatcacttct tccgtctttt cgcgggagcg gttgaaaata gagacggtat aaccacgact
    11821 ttcgatgttg agcgcaaggt tgcgccccat cactgccata ccgactacgc cgatctgttg
    11881 ctttgacatt gtttactcct gtcttctata ttattaaccg tctattaacc gttatattat
    11941 taaccgtcgt gattttcatc ttctaatcaa ggaatacata tggttacagc atgcttctta
    12001 ctttatagcg ataagtatgc cattgggtgt atccataaga actttgaatt ttggatactt
    12061 ttctggatta aaattagcat agcttcgttt agatataaca ataaacgaac tattaaaaga
    12121 tttcatcttc tctattaaat catcctgtgg gattacctca cactcgggat tactaatagt
    12181 ccaatcacta tatcttgcta atccaaagac gtcaccaatt gccatccctt tatagaagta
    12241 aataccatcc tccaagccta attggataag attatctcca tataactcac tatattcact
    12301 tgctttcttc atcagttcat agctatagcg actttctaag cgtgaattaa aattcaagta
    12361 ttcagaataa aaatatttga attcaaaatg caacgatcgc cccgaaaaaa aaataataca
    12421 cacaaggata aaatatcgtc ccaacttgta atttgtaatg ttgatatctt ttattaaact
    12481 tgcaattgag atcgcaacca gcaatactga caatgggtaa atgggtgata aatacctgtc
    12541 accttgcgta acagaaaacc aaaataagac atatgttaaa acaatcgaga aaaataccca
    12601 aagtatttta ttattcctaa gtgtaaagac agacagtata gcataaaaaa caacatcact
    12661 gccaacttta ttaattgctt gttgcaaatc taatgtggca ggacttatac catgtcgagc
    12721 ttgttctgct gtttgaactt gtagatcaat ttcattccat aggtagtgac caaatatatt
    12781 tcctccagca ggactaaaag gatcaccaga gatccaaaaa ctacggacat accaataaat
    12841 gccaccaaga attgttgcca agctaaaaat aagaaatgtc tgtatgtttc tctttttcgc
    12901 tatcaatata acaaatatga tggctccata tatggcacca aatagtttta cgccacaagc
    12961 aattccaata aaaaaaccac ataaaaaaat taattcattt tgtgttttat tgttttctga
    13021 gcgaatatat ataagaatac tggctgcagc aaaacaaaga tagcgttaaa tcaatatatg
    13081 cataaccaag atgatcttta agtggattga tagtaaatag taggattgta gcaataatgc
    13141 taggataata agttttcgta atttcccatg aacagccaac aagtccacag gctattacaa
    13201 aaaaacagat atttgctaaa aactgagcta accttacatc tcctaactgt aaacctaaag
    13261 ccattaaaag atctgcattt tgcgggaaca gaggaaaacg caaatattgt tcaacaacaa
    13321 ttgcatggtg ctcaactaat gaacgagcca aaggtaagtg atacatagta tcatcccaat
    13381 caccgggagc tcgcagggac gataacaatg taaccacaaa aatcaaaaca agaaataaaa
    13441 taacaaacag cgtactgaac tcaatattat ttattgccag ccatctaaat tgctgacgtt
    13501 taataatgct taaaacccaa cacaccgtaa aaattaaaaa gatagcaatc ttattaaatt
    13561 gcccgaccat gcccagaaag taaattatca aaatatttaa agacatccct atgcataggc
    13621 aataaaaaaa taaagaaaat ttgttaagtc ctgccacccc ctccttaaaa aaagatgaca
    13681 atacaatatt accaatgaca aaaaatgtca ttccaaaagc aaaacaatac acaacataca
    13741 aatacaacgg caattcaatc atagtaaaat actctattta cattatcagt ttatagtttt
    13801 tttcagaaag ataaaataat gccttcattt cacgtctagt tgttgtattt gaatgcaaaa
    13861 taatgccaca tacaaatgcc attattgacg ataacatgat ggaggcagtt aaaaaggcag
    13921 tcggaaatct tctcaccaaa cctgtatcaa tataatcaac aatgatgggc aatcctaaaa
    13981 ttatagacaa agcagcaagt aaaacgccga taatactaaa aaagtatagt ggccgttcaa
    14041 gcgagtaaag tttaattatt gttttcagaa tcttgaaccc atcactccaa gttgatagtt
    14101 tgctcacact cccttcagat cgctcgccat attttgtatt aacctcacca tacttcatac
    14161 gtaattctaa agcatgaatt gtaagctcag tttcgatttc aaatcccctt gatagacatg
    14221 gaaaagattt aacatagcgt ctggaaaata cacgataccc cgacaacata tcagtaaaat
    14281 tccctttaaa gatagtctga acagtaccag tcaacagttt atttcctcag cgatgccctt
    14341 ttctgtatgt attttgatct ccattctctt gacgacatcc tacaaccata tccaattgat
    14401 tttttatgag atggttaatc atttctggag cagaacttgc atcataagtg ccatctccat
    14461 caaccattag ataaatatcc gcatctacat ctgagaacat acgtcttacg acattcccct
    14521 tgcctttcag aggaacagag tgaactgttg ctccagcatc ctctgccact tgtgctgtac
    14581 tgtcagtcga attattatca aatacatata tagaagcagt tggaatattc gttttgaaat
    14641 catttatgac ttttgctatt gccttctgct cattataaca aggaataatt acagcgatat
    14701 taaaagagtg atacataagc ccctcatata aactatttag ctaatgtgca agtaattgtg
    14761 tttttaacaa atcgtttact aacgaaaaat gaaatatttt gatgcgcaat atcctaaaac
    14821 caaactaaaa agagaaaagg tgaacaatgt aaatattgga gataaagcta agagttcaaa
    14881 taaaacgccg aataaataac tcatcgcccc cataaaaaat ataaatataa aatatctagc
    14941 cttggttgca gatactttaa atgagcatct tgcatttgca aaaaaagaaa aggttgctgc
    15001 acatataaaa ccaacaatgt tacttcggcc ttgaccaaaa gctaaattat tataacacgc
    15061 ataaaatacc ccccaatgta aagcagtatt gacaattcca attaaaaaat aactaaaaaa
    15121 agaactggtc aataacttcc ctatttttaa catcctttat tttgctccag aagtgaggtt
    15181 attactaatt tggatatttt ctatagaaaa tacccataaa actacaacgc ctataataaa
    15241 aattcccatg ggtgtaaaaa gaaatggatt agttatagaa acggtaatac tgaaaaaaag
    15301 tattaacgac atcattaatg atgtttttgt actatttaaa aaatagcctt tcaaaaagat
    15361 aagcaacata ggaactaatg atataaatac accaataacg cctgttttct gaagtatctc
    15421 aagaggtact atttcaatcc ttaatcgatc taatataaat gaaccaaatc ctcttccaaa
    15481 caagaacgtc gctaaatcaa catttttata ataaaaatat aaatcattaa atctaacaga
    15541 gtcagaatct tcactgcgtg atccgaccat gtaaagcata attataaata gaacgctgcc
    15601 caatattatt ataatagtat ttaaatatag cttatgtaat ttaaaactgg ctaataacag
    15661 tgataatgtt gtaaaaagta atacacctct tgattctgtc aataatatcg caagcactat
    15721 tatgagacat tttaaatatt ttttgtcgac aaaactgata atcgcaccga caccaaaaaa
    15781 gtagaaggat ttggaaaaaa aagccccatc gggacgaaaa aagaactcgc tagtcaatga
    15841 taagtgttca taaattaaat taaaatttaa caaaccgaaa ttcaacaaaa ttatgtatga
    15901 aatatataac agtgatccat ataatgcaaa tgcattaact attttaataa aaatctcaca
    15961 taaaaatact tttaaagtat atcttagtgt aaatagatat ataagtgaca gcataaaaag
    16021 gtatggttgc acatcaaaca gtatatcatt tattttatta ccagctaata agccgacaaa
    16081 gaagccataa aaaggaaata aaagcaacca tataaaaaga ctgacacatt tttgggtgat
    16141 tgaaaaattg aatgtaacta aattaaaaat taaaagtata aaactaaata caaataataa
    16201 atatcgcaaa gggaatattc ccaatggctc cagtaatctt ccaccaccac caataatcag
    16261 ttcaatacat aaaaatgtta taaaaatttt atttatatta ttcatattcg taaggtgatg
    16321 ttttttaaaa tatgtttaaa atcaacatta ataaaatata aattttatca gcctttttcc
    16381 gccttaactt ccaaaaactt aaagccaaga tcaaattgcc tatataacct ttacccaatt
    16441 gattattcac aaagctatca tcagctaagg cattagctat tttagttact tcttttcgat
    16501 accacttgtt tctcaccaaa ccaagccgtt tgtattttgc tttaaaacta atgtttgctc
    16561 ccacttggtt gttttcatgc tgtctgtaca gcattgttgg ttttctatca atataccacg
    16621 aataatttct cgtccgcgca aatgaatata aaaaccaatc atgcagacag atatcttgtg
    16681 cagcattttt attatttatg atgaacttct taaattcaat aagcgtttct ttttttataa
    16741 catatgtaca ccccggacct gctgcttcaa aaaaataatc gaattgagtt tgcgactgag
    16801 ccttatcaac taaatttttt tgccctgatg gataatatgc aattacattg ctggaataac
    16861 aattcgcatt attaaatacc aagcagtcaa ttgcattttt tattttatct ttataccaaa
    16921 tatcgtcctg atcactaaaa cagatataat catagttttc aagatcgaca tctttaatta
    16981 atctataaaa atttttacct gcccccccaa acttattgac gcttggaagt atatttataa
    17041 ttgagggata tgataattga aattcttcac aaatatttaa tgtattatca gttgataaat
    17101 catctgatat aaaaattgag atgtctacat ctttttgttc aataatactt tttaattgtt
    17161 cctcaatcca gcattcgcca ttatacgtag ccataagtac agctactctt ttttgatcag
    17221 aactatgcat tataacgacc gcccccagta attcctctta ttccatccag cgtagccaaa
    17281 atatgttttt tcatatattc aattttattt ttttgagttg cgataattaa agtcgtatga
    17341 attaaagaaa aaacaacttc cctaatagag agtaaaagag gcacatgatt ttttctaagc
    17401 aaaagaaatg catttcttac tcgataataa cgacgtactg gcgagtgaat tggtatgtta
    17461 atcccacaga tttttactga cttatcccca atagcatgaa ccatttctat atcaggaatg
    17521 atatgaacta atatgccata gtttaagcat ctaagacacc attctgtatc gacataatca
    17581 ataaaaagtt cctcatccat cattccaaca atctcaaaaa cttcttttga aaccatggtt
    17641 ccggatgaga ttgttactga acttttaaac ggtgtctgtc cttcagtgac atgaattttc
    17701 tctcgaagac catttttttt gatattacaa ataggataat aatgtgactt atctctatca
    17761 ataaaaactg ggccagctat ttttgcattt tcattatcca tatagcagga aagttttttg
    17821 ataaactcat tgcgaatacg gctatcttga tcaaaaaaaa ttatcttctc tgcccctttt
    17881 tcaagagaat atcgaatccc aatattttgt gcttttgcta taccttcatt cctgccaaga
    17941 caaattaaat gaactttttc aatgtctgct aatttatctt tcaaggtgaa attagtccta
    18001 ttatcgacta ctacaacgta acaattttgc tcctttagtt cagtgatcag ttcattcaga
    18061 tttttaagtt cgggattata tgtcacaatg acagcgtaaa tattactatt cattatccaa
    18121 gtgactcagt aattggttaa ttttagtttt catatacaga acacattcta gtataaagcc
    18181 attgtaataa cagagcatct aaaattacac gtatgctcca ggatattgcg gcacccagta
    18241 acccatattt ttcgaccata aaatacaaaa gggctaaata aggaaccaat tcagcacaat
    18301 gaatcaaagc agtaatttta gattttccta atgcctggat tgcagagaat ggtatcattg
    18361 ccagcgcatt aaagaaaaaa ccgatcaata acacatttaa tatattaaag gaattaattt
    18421 ggtaattctc accaaaccat attttcaata caagccctga atataacaag cctatgatta
    18481 tcaccggcaa acagattaga aacattaaaa gcaatgattt atttacatta cgtttaaatt
    18541 ctttaaagtc tttgatgtta cttaaccttg gaaacactgc cctcccgatt gctgcaggta
    18601 ttattcctaa ttttagaata acctccgctg gtgcagaata aaatgcaact ttatctgccc
    18661 ccataatatt tgacactata aatcgatcaa aatacaccat gaccggactt atgatattac
    18721 ttactgtcat ccaaccgcca aagaaaaaga gtcgtttaaa ggttttataa caaaaatgaa
    18781 cccccgaaat tttaatatca ttcctgacat aataggcaga gactaaaatc gaaataactc
    18841 ttgcaaatat taaaccagca accgctgccg acaatgtagc actataaaaa acaaatatgg
    18901 ccggaattcc cgcgatgcaa gagcttgata tagatttttg aatatttaca atgccaaatt
    18961 tttcatcccc ctcaagaatg gctgaccata attgatttag aataaataat ggaatgcaaa
    19021 tagccaacag tttaaatgct agttgtatat cactatgttc aacaccagaa atttttagat
    19081 aattaacaat tccatcagag aaaatcaata ataaaaaagc accaaagcat gaaaatagca
    19141 ccaaaaaaga tgtacttgtt gaaattacct tggttctttc atgatgatta tcacgatgaa
    19201 ttgcaatttc tctaataaca gagcgagtca ggcctacatc aaaaattcca gcatacccaa
    19261 caagtgcaat tgcaattgta taaacaccga aaccttcagg ccccaaacct cgagcgagaa
    19321 atcctagcga ggggatagca attaaagttg gtattgcata gccaaaaagg ttccagacac
    19381 tattttttat tatgctcatt attacatttc tttatcatat aaaataatca gcatctacaa
    19441 atttttgacc attaatatct ttatcagaaa gcaatggatt ttgaaccggc cattctatat
    19501 ttatactttt gtcgctccat aaaatcccac gctcttgcat ttttgaatag aaattatttg
    19561 ttttgtagac aaattctgct tcatcactaa gaacgcaaaa cccatgagcg aatccttccg
    19621 gtatccataa ttgacgctta ttctccgccg acaaatttac accaacccac ttgccaaatg
    19681 ttggtgatga tttccgaata tcaacagcta cgtcaaaaac ttcaccgaca acacagcgta
    19741 ccagttttcc ttgagcataa ggttccagct gatagtgcag tccgcgtaac acacccttag
    19801 ttgatttaga atggttatcc tgaacaaact caacctttcg tcctacagcc tcttcgaaaa
    19861 ctttctgatt gaagctttcc ataaaaaagc cccgctcatc accaaatact tttggttcaa
    19921 aaattagcac atcaggaatt tcagttttaa ttacgttcat tttattaata acctttaatc
    19981 attttcagca gatactgacc ataagcattt ttcttcagcg gctcggctaa taccttcact
    20041 tgctcagcat caataaagcc tttacggtaa gcaatttctt ccgggcagga aactttcagc
    20101 ccctgacgct cttcaattgt tgcaatgaag ttgcttgctt caataagact ttgatgtgtc
    20161 cccgtgtcca gccatgcata accacgcccc atcatagcga cagacaaacg tccttggtcc
    20221 atataaatac ggttaatatc ggtaatttcc agttcgccac gtgcagatgg tttaaggttt
    20281 ttcgccattt ctacaacgtc attgtcatag aaataaagcc cagtaaccgc atagttactt
    20341 tttggttcca gcggtttttc ttccagacta attgccgtac cgttatcatc aaattccacc
    20401 acaccgtagc gctcaggatc atttacgtga taggcaaaaa ccgttgcgcc actctctctg
    20461 ttaacagcgg tatccattaa tttcggcagg tcgtgaccat agaagatatt atcaccaagt
    20521 accagcgcac aatcatcacc acctataaat tcttcaccaa tgataaatgc ttgcgccaga
    20581 ccatccggac tcggttgtac tttatattgc agattaagcc cccactggct tccatcacca
    20641 agcaattgtt gaaaacgtgg tgtatcctgc ggcgtactga taatcagaat atctcgaata
    20701 cccgccaaca taagcgttga aagcggataa taaatcatcg gtttatcata aatcggtagc
    20761 agttgtttgc tcacagccat tgtcacagga taaagacgag tgccggaacc accagccaga
    20821 ataatacctt tacgcgtttt catttcacta ttccttttaa ttcatctcgc tctggcatca
    20881 tgagcgagat gcaataattt gttaaattgc cgtggtcgta aataattcgt tgagcatacg
    20941 tttcacgccc acctgccagt caggcaagac aagcgcaaag ttctgctgaa atttttcggt
    21001 attgaggcga gaattatggg gacgacgtgc tggtgtggga taggccgttg tcggcacggc
    21061 attaagtttg ttaagagcaa ggttgatccc cgctctgcgc gcttcttcaa ataccagagc
    21121 ggcataatcg tgccaggttg ttgtaccacc agcaaccaga tggtacaaac cagcaacttc
    21181 tggtttattt gcggccacgc gaatggcatg agcggtgcaa tcagccagta attcagcccc
    21241 cgttggtgcg ccgaattgat cgtttattac agccaactct tcgcgttctt ttgccaggcg
    21301 caacatcgtt ttggcaaagt tatttccttt accagcgtat acccagctgg tacggaaaat
    21361 aagatgcttt gcgcaatgtt cttgtaacgc tttttcccca gctagcttgg tttcaccgta
    21421 gacatttagc ggtgcggttg catccgtttc cagccatggc gtgtcaccgt tgcctgggaa
    21481 aacataatca gtggagtagt gtataaccca ggctccgact tcattagctg cttttgcaat
    21541 cgcttcgacg catgtcgcgt taagtaattg cgcgaaattc ggttctgatt ccgctttgtc
    21601 tactgcagtg tgagcagcag cattaacaat aacatcgggg cgaatttttt tgacggtttc
    21661 agccacacct tcggggttgc tgaaatctcc acaataatca gtggagtgaa catcgagcgc
    21721 aatcaagtta cccagaggag ccagagcacg ctgcagttcc caacccacct gccctgtttt
    21781 gccgaaaagc aggatattca ttactgacgg ccctcatagt tttgttcaat ccacgactga
    21841 taagcaccgc ttttcacatt ctcaacccaa tttgtattag ccagatacca ttccaccgtt
    21901 ttacgaatcc cgctctcaaa cgtttcttgc ggtttccagc ccaattcgcg gctaatttta
    21961 tcggcatcaa tggcgtaacg gcggtcatgc cccgggcgat cggcaacata agtaatttgc
    22021 tcacgataag atttctcttt cggtacaatc tcatccaaca aatcacaaat agtgagcact
    22081 acgtcgatgt ttttcttttc gttatgtcca ccaatgttat aagtttcacc cgctttacct
    22141 tcggttacga cggtatataa cgcacgagca tgatcctcta catacaacca gtcgcgaatt
    22201 tgatcccctt taccataaat aggtaatgcc ttaccttcca gagcattcag aataaccaat
    22261 ggaataagtt tttccgggaa gtgataagga ccataattgt tcgagcaatt agtcacaatg
    22321 gttggtaaac cataagtccg cttccaggca cgtaccaaat gatcgctgga agctttagac
    22381 gcagaatacg ggctacttgg cgcgtaagct gtcgtttccg taaatagcgg taacgcttcg
    22441 ttattatttg cttcatccgg atggggtaag tcaccataaa cttcatcagt agaaatatga
    22501 tgaaagcgga aacttttttt cttttcatca ttcagagcag accagtaatt gcgcgccgct
    22561 tctaaaagaa cataagtccc cacaatattg gtttcaataa atgccgcagg gcccgttatt
    22621 gaacggtcaa cgtggctctc tgccgccagg tgcatcaccg cgtctggctg gtgctgtgcg
    22681 aaaatacgcg acatggctac agcatcgcag atatctgcat gctcaaaagc ataacgttca
    22741 gaatcagaaa catcagcgag tgattctagg ttcccggcgt atgttaattt atcgacatta
    22801 acaacactat cttgcgtatt atttattatg tgacgaacaa cagcagaacc aataaaccct
    22861 gcgccaccag taacaagtat cttcactttt ttattccata tagctataga gcatgctgtg
    22921 aaatagactg ctccagattt gattaataga ggtattaata cacgctaccg cccctggctt
    22981 aacagctacc agtgcactgc atgcatctca acgatgtggc gagcgttagc cactcgcgcc
    23041 aaatccgaaa aattcaaacg ttaattgtct taccaaacag ccgtagaaac aaggaaaatc
    23101 ctggaaaaat ttgaataata tgagctatgt aacttcttgt ttttataggc tttataaaaa
    23161 cggcaacggc aggaaaattc aatgagcaaa acttatgcca cgaatcttca ctgccgttat
    23221 aattttctta tcaaccgtta catccggtca gattttcatt attcgcttaa cagcttctca
    23281 atacctttac ggaacttcgc cccttctttc aggttgcgta ggccatactt cacaaacgcc
    23341 tgcatatagc ccattttttt gccgcagtcg taactgtcgc cggtcatcag cattgcatca
    23401 acggattgtt ttttcgccag ctcggcaata gcatcagtca gctgaatacg tccccatgca
    23461 ccaggctgag tacgttccag ttccggccaa atatcggcag aaagcacata gcgacctacg
    23521 gccatgatgt ctgagtccag cgtctgcggc tgatccggtt tttcgataaa ttcaacaata
    23581 cggctgactt taccctcacg gtccagcggc tctttagtct ggatgacgga gtattcagag
    23641 aggtcacccg gcatacgttt tgccagcacc tggctgcggc ccgtttcgtt gaaacgtgca
    23701 atcatggcag caaggttgta acgtagcggg tcggcgctgg catcgtcgat cacaacgtct
    23761 ggcagtacca cgacaaatgg gttgtcacca atggcaggtc gcgcacacaa aatggagtgg
    23821 cctaaaccta aaggttcgcc ctgacgcacg ttcataatgg tcacgcccgg cggacagatg
    23881 gactgtactt ccgccagcag ttgacgcttc acgcgctgct caaggagtga ttctaactca
    23941 taagaggtgt cgaagtggtt ttcgaccgcg ttcttggacg cgtgagttac caggaggatt
    24001 tctttgatcc ctgcagccac aatctcgtca acaatgtact gaatcattgg tttgtcgacg
    24061 attggtagca tctctttggg tatcgcctta gtggcaggca acatatgcat cccgagaccc
    24121 gctacaggaa taactgcttt taaattcgtc attatttcat ccacctgtaa aatggttgct
    24181 gaattatagc ttgttcgatt tttttcgcca gcatcaatta ccctgaattg attactgaat
    24241 tacttgtgat gttacgccgc ttcgttgtgg attgcagtag cattgttcct aagtatggct
    24301 ccatttttcc aggaatggtc gcaaatctac tccctccgtt ccggcaatct aaagttaatc
    24361 ttttccacat taacaatatg gtgattaatc ctgtcgatat cgacggagct ttgccctctt
    24421 tcattcaccg catgaacatt tgcaagagac agcagtgttt cttttttcgc cataaagacg
    24481 ccgcgaacgt ctttgcgcat gtcgaagttc atgctcaatg ctggtccaac tgaggattcc
    24541 tgcatcacat tgatatttcg tataaaaaga tgttgcggct tgttgtgtaa ctccagcgag
    24601 gcacgcttca tctcaatgtt agttagcgac acaaaggaga cggcattccc ggcggagatt
    24661 tggatgccgc gcaatttata agcaagatgg gtattatcca gttgaatatt attcactcgg
    24721 aaattttgcg gtatcgagag atatttgcct ttaattaccc catagccgat taacatcccg
    24781 gcgctattaa tcatttcaat attatcaatc acgaaattgt cacaaccgta aatagcgact
    24841 gtcgcgttat caatgcccgc tttcttactg aaatccggcg tgatattgcg ggcattgata
    24901 ttacgaataa caaaatgttt accattttca acatggttca actgccgaca atccgatccc
    24961 gtgatattcg ccacgacaaa gtttttcact gcctggtctt ccgggtagtt gttatcataa
    25021 gtgcttcccg caaggcctat gccgatgccc cagttgattt tgccgttggt acagttgata
    25081 cgctcgatga catggtcaga tatcaaaata tcactgtcgt taattgccac gttccattca
    25141 atggcgtcgc cctgtaagtc gctgaactta cagttggtaa tgttggcacc gataatctgg
    25201 ttatgaaatc cctggcgtaa gatggcgtaa ttagcgtggc tgacagtgag gttatcgatg
    25261 gtcaggttgc gcatgacccg tttgtttttg ccgccaatat aaatctgcat taccgggcca
    25321 aagccgctca tcgccagccc tttgatggtg cagtcagaac cacgcacatc cagggtaatg
    25381 ttatgcatac cgccgccctc tccccctgtc acctggctgc cgtcctgtaa gacaaatcgc
    25441 cctctgccgt tgccgcgcag gcttccaagg atgtgtaacg ttttgccggg agggatgaag
    25501 atgccggtgt tgatattgtc acaaacgaat ccggcaggca cgacgaccgt ttgcccttcg
    25561 ctgaaggctt gtttaaatga ggcgatccag tcatgtgggt tgtagtcgtt aatgttaacg
    25621 ctttgtcggg cgggaagtgc gcgagcgaaa ggggtatgga ggaaggcaag cgccgagctt
    25681 gccgtcagga aggtgcgtcg ggagagtgtt ttaaatggca tacgttctcc tctataaagc
    25741 ctgcagcaag ctggcgagtt ctcgattaat cacctgctgg ttaaaatcgt gttcgacttt
    25801 ttcgcgcgca cgtttgacga ccggagccag ttcgtcggta tccagttggc taaacgccgc
    25861 cagacgttgt gccagtgcgc gagcatcgtt ctcaggcacc agccagccgg atttatcggc
    25921 ctccaccagt tcaggaattc cgctatgcag agtagaaacc accggaatac cgaccgccat
    25981 cgcttccatt agcgccaccg gaatgccttc catatcgcca tccgcgcccg ttaccgatgg
    26041 caacaggaag acatccgcgt cgtcgagcat ctccttcact tcatagctcg gtttaaagcc
    26101 cggcatctct accacatctt ccagttgata ttgttcgatg agggtgcgca ggcgtcgttc
    26161 ccacaggcca atgccgagga tgcgatagcg aaatgccacg ccctgctctt tcaactgacg
    26221 gcaggcttca atcgccacat gcaggccttt tttctcggtt aagcgtgcga cagatatgat
    26281 ttccagcggc gttgcgggcg ctttcaccgg acgcgggcta aagcgcgtca tgtccacgcc
    26341 catacgcgat acggcgattt tttctctggg gcagcccatt ttttgcagcc ttccggccca
    26401 cagatcgctt atcggtaaca tcaggtcgcc acggcgaaac agttgttgat attcgggagt
    26461 gtagtggttg agcacttccc gactggagat atcaataccg tggaagatgg tggcaatttt
    26521 gccgcgaatg atacccagtt cgcgtagttt tgctgcggtt acccccgccg ggccaaaatg
    26581 agcgatgaac acatcggcgc gaaacggtgt tgcgacctga ccacaaatgg cagacaaaat
    26641 caggttccgc gactcggcac catagcgttt gaggttgagc gcatgccagg tatttttacg
    26701 atgaatgcca cgtaaggtct ggctggcgcg gtggcgcagt ttcgctactt tgcctgcagg
    26761 ttcgtcctgt aaccagcggg ttctggcgac aaggttgtat ttcgtccatg ccgcgtgggt
    26821 gttctctgta tcgccttttt gcaaagcgac aatctccact tcaaagccca tatcaataaa
    26881 cgcggtaatt tgattgagga cgaaggtttc cgacgacagc gggaatttca ataagaagaa
    26941 gccgaccttc atttcacctc cccgatgcgc tcaagcacag actgcaccat ctgcattccc
    27001 gtctgacgtt cgcgactgac ggcttcgtta aatcgagcgt tcagcaccgg aagctggcct
    27061 aaggtatccg caatcatcgc ttgcaggctg ccgtccagta aatgacggat atcaatcgcc
    27121 atctctggca gtcccagctg ctgcataatc ccggcggatt tatgttcata gttgatggca
    27181 atcgccggag tagcaaagtt cattgagata atggcggagt gcaggcgcgt accgacggtg
    27241 agttcacagg ctccgagaat tttgcccatt tccagatcgt tgagttcatc catcactacg
    27301 tggtaacggg caggatcgct gatgtgctgg cgcagattga gcgccaccat gcggtcgtct
    27361 ttgttatagc tgtcgatacc cgtacaggtg gagagcgcaa tcacctgata gccttcatcg
    27421 agaatgcgat tgaccacccc ggcaaaggct ttttcatacg cttgttgagt ggtgccgaga
    27481 cgtttgtcga acggtgccag ttcgcgcagg gtaattgcca ctgttttctg ttgtgcggca
    27541 acgtccagcc agtgttgaac ggcatagctg gcggtgaagt cttcggtgtg gtgatcgacc
    27601 agccacgcgg tatcgacgcc atgttccact tttgcggtgg tgatattgct gcgtttcatc
    27661 aaatcaaggc tgaccgattc gcgcaggatc agcgcgtcgc agtgaccaaa gacatagttc
    27721 gccagttggt taaattgctc atcctggaag gggccgacgc tgtgaccaat cataaacagc
    27781 ggctttttcg ccataaatgt gcaaagcgca tgttcaaact gcggcacgcc gtagagatcg
    27841 acaaaaaacg atccgccgac ctgaataatg gcgtcgtaac ctgacagcag gcgcacgaag
    27901 tcggtgaatc cctgggcgat ggcgatattg cgcagcttgc cagtgtcggt gacgcgtgag
    27961 agcaatacct gatgctggta gctgcgacgg aggacttttt taacgcgccc gacaacgccc
    28021 gccgcgctat tgtgttgtgt catctgcagg aacagcggat cgcccattac cgggcggttg
    28081 agcagccagg aagaactgac cggatagcgg ctcatcacgt ccacttcggt atgtggattg
    28141 aggatgttga tggcatcaag taagccgcgc aagatggcgc tgtcgccacg attgccgcaa
    28201 gtgtggttgc ccagaataag taatttcatt ttttcctcat aaatttgatg ccaggtgagg
    28261 ctgtgtttgt tgtgttcgtt gccgtttttt gcctgatgcg acgctggcgc gtcttatcag
    28321 gcctacaggt cccgagcaca gaaccgtagg acggataagg cgttttacgc cgcatccggc
    28381 aaccgttgtc ggaaccgaaa acagcaattc accccgcccg taaaagcatt ttcatttttt
    28441 cgctgcgaca aaactgacgc ttcacttcca ccaccagcgg atggcgtgac agcacaatca
    28501 tcaccacaaa cgccagcacg cccgcggcta tttgcaccgc cagcagcatc cccagcgcca
    28561 gttgcccttt cagcacaatg cccagcgcat aactgaccac cagcgtcggc aacgagagat
    28621 aaaacggcag ccacaaactc aggatgtact ggcgataact ggaaccaaga accggtttaa
    28681 tcatcacgaa gtaactcaga atggtgttga taatctgcac cagcaggaaa ccgagcgtga
    28741 cgccgattgc gcccgccatc tgcccaccaa taacaatcgc cggaataaac agaaatgttt
    28801 tgaatacatt gaatttaaag ctgatatcga cccgcgcttt cgccatcagc agcgaaccaa
    28861 tcgggttacc cacggagcgc agcagaccca caatacacag caattgcagc accggaataa
    28921 tgctgttcca cttttcgccg aataccagcg gtacgaaatt attcgacacc accatcagcc
    28981 ccagcagcgc cggaaagttg ataatcccca ctatcgacag cagcttgtag aagttaacac
    29041 gcagcttttc ggtgtcgtcc tgaattttgg caaacgccgg aaacaacacg cgggtgatga
    29101 ttgggttcag cttcatcggc ggtacgacgg ccacgttgta cgccaggttg tatccccctg
    29161 ccacgcccgc gccaagaata cgcgccagca ccagcgttga aaggttggtg ttgagatagt
    29221 tgatgatgct gtccgccgtc agccaggcac caaaacgtaa gttcggtgcc acagacgcca
    29281 gcgagaagtg cagaccgggg caataaattt tgcgtccaaa gtagccaaac agcagtgttc
    29341 tcaccgcact attgaccaga taaccgagga tcgcggtcat cgccagcggc cagaaatggg
    29401 cgctaactac cgtaaaagtg aagcccgcca gcaccgcgct ggtttcgatc atgccgattt
    29461 tgttgaactc cagctccttc tgcatcaacg cgcggaactg ttgcccgtgg gggatcacca
    29521 caaacgccag cgacaatgtt ttaatcaacg gtgccaggtc cgggttattc agcacgtcgc
    29581 cgatgacatc actcaacaaa aacaccgaca cacataccac aagccccagc ccgacgttca
    29641 gccagtacag cgtggtgagt tcaaggtgac tgatttcttt tcgctgaata atcgagttag
    29701 cgataccgaa gtcagaaagc gtatctgcca gcgcgataat caccagcgac acagtcagca
    29761 ggccgaactg gtggttgtcg ataatccgcg ccagcacggt catctgcacc agcccgaggc
    29821 cgatgatgat caccgtggca atagccgacc acttcgcgcc gctgatggtt ttttcacgta
    29881 agctcatatc aatatgccgc tttgttaacg aagcccttga ataccgtcag gaaaacgatt
    29941 ttgatatcga accagacgct ccattcgcgg atgtactcaa ggtcgaactc gacgcgtttt
    30001 tccattttct ccagcgtgtc ggtttcgccg cgccagccat taatctgcgc ccagccggta
    30061 atgcccggtt tcactttatg gcgcagcatg tagccttcaa tgagctggcg atactgttcg
    30121 ttatgcgcta ctgcgtgcgg acgtggaccg acaatcgaca tccccccggt cagcacattg
    30181 ataaactgcg gcaattcgtc cagcgaggtg cggcgcagaa agttccccac tttagtgacg
    30241 cgcggatcgt tctgcgtcgc ctgggtcacc actttgtcgt tctccatcac tttcatggaa
    30301 cggaacttcc acactttgat cggcttgcca tccataccat agcgagtctg gcggaaaata
    30361 accggccctg gtgaactgag tttcaccgcc agcgcaatac agcacagcac cggggagatc
    30421 agcagcagaa taagcgtcgc cagcacaatg tcttccgcac gttttagcag gcggttaccc
    30481 cccgaaagcg gcgtgtcata cagcggcacc accggaacgc cgttcatctc ttcgagacgt
    30541 gaatggagaa tgttgaaggt aaagacgtcg gggatcagca gcaccgaaca ggtggtatcc
    30601 gccagttgat gggccagttt tttcactcgt gcgccgtcgc acatttgcat cgcgatatag
    30661 acgttatgaa tcttgcccgc ttttgcatcc tcgaccagct gttgcaggtt acccgcccag
    30721 tcgttagaaa cgccgcccgg tttcgtgtcg tggtaaacgc ccaccacttc aaaccctaac
    30781 cacggctgat tacggaaact ctccatcagc atttgtccgg cggctaaatc ccccgctacg
    30841 gcgaccatcc gcttgttgta gccatgatta cgcagccagc ccgccccaat gcgaatacat
    30901 gaacggcaaa ccaccagtcc gatgctggtc agcccatacc acgccagcca gattttcagt
    30961 tgcgtgtcga aatcattgtt gaacgccacc agtccggcgc tgaaaatcac gcttaaggtc
    31021 cagttttgta gcaacagggc aaattctgtc gctgcccgaa caccgcgcca tgagcgataa
    31081 aaatcggtga tgccgcccag catctggaac accaccagcg taatcagcgc caccaacagg
    31141 tgcatgtaga ggaatgacag tccgctgact tcgcaaacca gccatagccc ggcaaacatg
    31201 atggtgatat ctgaaaagcg ttgcaccata gagattaacg atgcattggt tttcgctcgc
    31261 tcgcgctttt ttagatttgt catcgttgtt cctgttatta gccccttacc cgcagtgggg
    31321 taagggaaga tccgacatta ctcgttcagc aacgtcagca gagttcgcgt tcgcgcttcc
    31381 atcagcggca catcaccgcg cgattccaca ttcaggcgca ccaccggttc ggtattggag
    31441 gtgcgcaggt taaagcgcca gtcggcaaag gtcatgctga tgccatcgct gcgatccacc
    31501 gccagcgcct cacggctaaa atgctgttcc acgcggttaa tcgcctcaac gggttgcgcc
    31561 agtttgctgt tgatctcacc gcttgccgga aacgccgcca tccggtcgcg caccagttcg
    31621 cccagcgttt ttcctttcag gcacaccagt tcggcgacca gcagccacgg gatcatgccg
    31681 ctgtcgcagt aagcgaaatc acggaagtaa tggtgggcgc tcatttcgcc accgtagatg
    31741 gcgtcttcct tgcgcatccg ttctttaata aaggcgtgtc cggttttcga cattaccggc
    31801 gtgccgcctg cagcagtcac cacatcaata gtgttccagg agagacgcgg atcatggatg
    31861 atcttcgcgc cgggattttt ttcgaggaac gcttctgcca gcaggccgac aatgtagtag
    31921 ccctcgataa actgcccttt ttcgtcaaac agaaaacagc ggtcaaaatc gccatcaaag
    31981 gcaatgccca tatccgcgcc gtgtttgatg accgcattac gggtgtcgtc gcgacattcc
    32041 ggcagcagcg ggttaggaat accgttgggg aaattgccgt ccggtgtgtt gtgtactttg
    32101 attaattcca cgggcgcgcc gagggcttta aagcgggctt caatggcgtc caccaccgga
    32161 cccgctgcgc cattcccgga gttgatcacc agcttgagcg gcgtgagatt tttgacattg
    32221 atataaccga acaggtgatc aacgtaagcg tcacgcaggt tgatttgctg atagcgaccg
    32281 cgtttggttt catcgacggg aggaaagtcg ttggcttcag ccagacgctg gacgtcgcgc
    32341 agtccggtat caccgctgat cgggcgagcc ccctcgcgca ccagcttcat gccgttgtaa
    32401 tccatcggat tatggctggc agtaacttca atgccgccat ccacgccgag atggaacgtg
    32461 gcgaaataga tctcttcggt gccggacata ccgatatcca gcacatcgac gcccgcatcc
    32521 tgtaaacctt ttgccagcgc cagttttaag gtttcgctgg tgagacggac atcaccgcct
    32581 aacacaatgg ttttcggttt gagaaattcg ccataggcgc gaccaatgcg ccaggcgata
    32641 tcttcattca gttcttcgcc taatttcccg cgaatatcat aggctttaaa gcaggttaat
    32701 tttttcatgt cgttaccctt tttcaggcaa tagttggccc tgaccgaaac ggccaatttt
    32761 atttttgtcg ttatttacac cgcggtttcg cattcattgc ctgatgcgac gtttacaccc
    32821 gtccgtagcg atccgcgaag cgcaccacat catcctcttc gagataagag ccggagcgca
    32881 cttcaattaa atcgagcgga attttccccg ggttttccag gcagtgcgtc gcccccagcg
    32941 gaatataaat ggactcgttt tcaccaagca gtttgatatc accatcaatg gtgacttttg
    33001 ccgttcccgc gaccactacc cagtgttccg cgcggtgatg gtgcatctgt accgacaaac
    33061 cctcgcccgg tttcacggtg atgcgtttca cctggtagcg gtcgcccgcg tcgatagagt
    33121 catatttgcc ccacggacga tacacttcgc gatgcacccg atgctcatgg tgaccatcgg
    33181 ctttgatctg ctcgaccact tttttcacat cctgtaccgc gttacggtcg gcaatcagca
    33241 ccgcatcttt ggtctgcacc actaccagat ctttcacccc gacggtggtg actaggcctg
    33301 attcggcata cacatagctg ttttcagttt tgtgattaat cacatcgccg tggcaaacgt
    33361 tgccctcggc ggtgtgggcg ctgatctccc ataatgaaga ccaggaacca acatcgctcc
    33421 agcccgcatc catcggcacc acaacagcat ctgccgtacg ttccatgacc gcgtaatcca
    33481 ccgactcttc cgggcaggcg agaaacgctt cttcatccac acgaataaaa tcgagatccg
    33541 gatcgacggc gctcatcgct ttttcgcagg catcgaggat atccgggcga tattttttca
    33601 gttcttcgag atagcgtccg gcgcggaaca ggaacatgcc gctgttccag taatattcgc
    33661 tgcttgctac ataggcctgg gcggtttcca gattcggttt ttcgacaaac tgcgccactt
    33721 caaaggccac cgtatcctgc tcacccgcag acacttcacc gcgacgaata tagccataac
    33781 cggtttctgg tagatccggc acaatgccaa aggtcaccag cttgcccgct tcggcataag
    33841 gcatggcatt acgcacggcg gcacggaacg cgtctttatc ggcaatcaca tgatccgccg
    33901 ccaataccag cattaacggg tcgctctccg ggctatgacg ttttgccgcc agcgccgcca
    33961 gtgcaatggc aggtgcagtg ttacgccctg ccggttcgag aataatgttc tcggtgagtt
    34021 tgttcagttg acgcagctgt tccgcgacaa taaagcggtg ctgctcattg caaatcacca
    34081 ccgggctttc gcactccacg ccgttcaggc ggcagatggt ggtttgcagc atggtgagat
    34141 cgcctttcag gcataaaaac tgcttggggt aaagtacgcg ggaaagcggc cataaacggc
    34201 taccggagcc acctgccatc acaactggat agagtttcga ctgcgccata attatccccg
    34261 aatatcattt ataaattgac gtaacacgtt ctctttatcg agcgtgcgtt cggcatattc
    34321 acgtgccacc gtgttatgtt tgggcagcag gagcgcctga cgaatccccg ccaccagcgc
    34381 ctcgaccgat tccggttcta cgcaaaccgc aatacccgga aaggtttcgc aaagctgccc
    34441 cagttctgtg tgggcctctg cggtaatcac cgcgttaccg cctaccgcca gaatattggt
    34501 cagtttcgac ggcaatacgg catctgccgc gccgcgtttt tgcaccacca gatggcaatc
    34561 gcccatcttc agcagtgcgg gtaaagcgtc atacgattgc agcgggaaaa attgcatgtt
    34621 gcgcagtcca cgctgctgcg ccattttttc cagccgcgct ttgccgccgc cctgcccgac
    34681 aatggcaaaa agcagcggtt catcgcgcag gagatcggca gcttcaataa cgttttccag
    34741 cccctgcttt tcaccaatat tgccggagta aagaatgatt tttttgttat ccggcaggcc
    34801 aagctggtta cgaagggcat caacatcggc gtctgcaaca tgctgaaaac gggcaatttc
    34861 cgaccagttg gggaagaaga tgacgttatc cgccgccacg cctttttcga tggctttatt
    34921 catcatcgaa cgcgaaatcg tggaaacgtt atcgacgtta tgcagtccgc tacgttcgaa
    34981 cgccgttgcc agctgtgcca ctttgccgcc tttgcctttt ccggcaaggc ccagccccag
    35041 catggcgtcc acttcgtaat cctgaatatg cagcacggta cgcgcaccag agagcttcgc
    35101 cagcaggcgc attcccggcg tgcaaaacag cgttggcact acgccaataa tgcgatccgg
    35161 cttccagcga cgttgcgcca tcagtggaaa gaaactgctg acggcaaaac tgccgagatg
    35221 caacaagcgt ttcagggtgc tcggctgttt tggcacgtac agcgggcagc gccacaccgt
    35281 ggcggcccct tcttctcgtt tgtagcgcca ggcggaatag ttctcaccca cctgccattg
    35341 cgggtagtaa ggcggtgcgg taatgacccg cacctcatga ccttgtgccg ccaaccattc
    35401 caccatctcg ccggtgtatt tgccgatgcc ggttaactcc ggcgagtagt taatgccgta
    35461 gaccagtatt ttcataatcc gggtactccg gcacgcttct cagcgagaaa ataggcgcgg
    35521 ctgttagcgt gaacatcgtt actggcgagc agcgcgtccg gcgtcagcca gcggtaatcg
    35581 tcatgctgct catccggcag taacagctct tcttccgcta ctctgaagcg aaaaccgagc
    35641 accacatagt gagtggtgaa atccgtgccg gagaagttat cgtcataaaa atgctgccag
    35701 acaccgtaaa actggcctgc tgttatcggc agacgcagcc ccagttccgc catcgtcagc
    35761 cgctcaaatg cggcttccag cgtttcgtct ttctgcacgc gccctcccgg cacaaaccag
    35821 taaccctgcg ccgggcggtt ggttcttttg ccaagcagaa actcgccgcg actgttctcg
    35881 acaataaagt cgagagagac aagcggtgtg gagcgcacta cagtggcaaa gtcttcctga
    35941 cgtaaaaaca tcattccccc cgaaagcggt cttgattctc aaggaaccac tggtaagtgc
    36001 tggcaagccc cgcttccagt gagatttcgt gataccagcc aagctgatgc aggcgcgtca
    36061 catccagcag tttgcgcggc gtaccatccg gtttgctggc atcaaaaacc acccgacctt
    36121 tgtaacccac cactttggcg atggtttgcg ccagctcgcg gatagtgcag tcaacgcccg
    36181 tgccgacgtt aatgtgcgac agcatcggtt gggtattctc cagccagact tcatgcgcca
    36241 gctccatgac atgaatgctc gccgccgcca tatcatcgac gtgcaggaat tcacgcatcg
    36301 gtgtaccgct gccccatacc accacgtccg gcgcattctg tgccgtcgcc tcgtggaagc
    36361 gacgcagcaa tgctggtatc acatgggagt tactcgggtg gaagttgtca tgcggcccgt
    36421 acaggttggt cggcatgaca gagcggtaat cgcgtccata ctggcggttg taagattcgc
    36481 acagtttgat cccggcgatt ttggcaatgg cgtaaggctc gttggtcggc tccagcgtgc
    36541 cctgcaataa ctcgctttct gccatcggct gttttgccag tttcgggtag atgcaggacg
    36601 atccgagaaa catcagtttg ttcacgtcgt tctgatgcgc ggcgtgaatg atgttgctct
    36661 caatcatcat gttctggtag atgaaatccg ccggataggt gttgttggca acaatgccgc
    36721 ccactttcgc cgccgccaga tagacctggt caatgcgttc gctggcaaag aaatcatgca
    36781 cggcgcggct gtccagcagg ttcagttcat cgcgggtacg taataccagc tccacatcac
    36841 cgcgctgttc gagctgccgc ttgatggcag aaccgaccat cccacaatgg ccagcgataa
    36901 aaatgcgttg tttacttatg cttatgactc cagcgcgatc gccacgtcgt agccgtgaga
    36961 tttcagcaga gagtgttttt tcgccgcttc gaggtcatta gccaccattt cagacaccat
    37021 ctctctgagg gtgatttccg gtttccagcc cagtttttcg tgcgctttgg tcgggtcgcc
    37081 gagcagcgtt tcaacttctg ccggacggaa gtaacgcgga tcaacggcga taatcacatc
    37141 acccggttta acgcccggcg cgtcatgccc ggtgacggaa accacaatgc ccttctcttc
    37201 aacacccgtg ccttcaaagc gcagtttgat gcccagctgt gctgccgcca tttccacaaa
    37261 ctgacgtacg gagtactgaa cgccggtcgc gataacgaaa tcttccggct gttcctgttg
    37321 cagcatcatc cactgcattt ttacgtagtc tttggcgtgg ccccagtcac gcagggaatc
    37381 catattgccg aggtacaggc acgactccag cccctgggcg atgttggcga ttgcgcgggt
    37441 gattttgcgg gtaacgaagg tttcgccgcg gcgcggggat tcatggttga agagaattcc
    37501 gttacaagcg tacatgccgt aggattcgcg gtagttaacg gtgatccagt aggcgtacag
    37561 tttggcgacc gcatacggag atcgcgggta gaacggcgtg gtttctttct gcggaatttc
    37621 ctgcaccaga ccatacagtt cagaggtgga agcctgatag aaacgagttt tcttttccag
    37681 accgaggaag cggatcgcct ccagcaggcg cagcgtaccc atcgcgtcga cgtcagcggt
    37741 atattctggt gactcaaaag agaccgcaac gtggctcatt gcgcccaggt tgtacacttc
    37801 atccggctgt acttcacgca aaatgcgcgt caggttagag gtatcactca ggtcgccata
    37861 atgcagatgg aatttcgggt tgcaggtgtg cggatcctga taaatgtgat ccacgcgctc
    37921 ggtgttgaat gacgatgcgc gacgcttaat accatgcacc tcgtaacctt tttccagcag
    37981 aaactctgcc aggtaagaac cgtcttgtcc ggttacaccg gtgatgagag cgacttttga
    38041 catgtcttat tcctctgtat ttttgaattt attcagtttc aacgcgttcg cgtatcacca
    38101 ctgcgggatt cccccggcaa accacatttg ccggaagcga tttaaaaaca ctgcttcgtg
    38161 cacccacgac ggtgccgtcg ccgattgtga cgcctggggc aacaaagaca tcggttgcca
    38221 gccagcattt ctcgccaatc acaataggcg tggcgttaat ggtgaaatgt tgacttgcat
    38281 ggtcgtggct accggtgcat aaataacttt tttgcgatat caccgaatgt gcgccaatgg
    38341 ttatttcacc gagggtatat aaattgacgt catcgccaac ccacgcgtaa tcacctaagg
    38401 ttaatttcca cggataggta atttttactg acggacgaat aactacgttt tttcctattt
    38461 ttgctccgaa taaacgtaat aaaaaagccc gccagcgata caatacttgt ggcgaccagg
    38521 caaatattgt tgcctgtact gcccaccata attgcacttt aatagcgttg ccgccccgga
    38581 accctttcgg caccgagaaa ccgcttaaat cttgcattgg ttttccttat attcagactt
    38641 tgttatataa ggctttagtc ttacctgtcg tacgttggcg taaatgccag gataattctg
    38701 cccagaagcc aggtacatgt aatatctgcc gttggacttt tttggcgtca gcac
//
LOCUS       DAJVQH010000019        37715 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJVQH010000019 DAJVQH010000000
VERSION     DAJVQH010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.019"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..1995
                     /gene="speF"
                     /locus_tag="OQM87_001143"
     CDS             <1..1995
                     /gene="speF"
                     /locus_tag="OQM87_001143"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCS1507364.1"
                     /translation="RVPAEYLPRISGVFEHCESRKEFYGRQLETAASHYETQLRPPFF
                     RALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLCNADVAMGDLL
                     IHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHKSNH
                     HGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQRAKEARPFRLA
                     VIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLNEND
                     PGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYPLF
                     AALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYET
                     AQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPYKLLLTTPGIDARNGEYEAFGVPAT
                     ILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLAEVL
                     PSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHVSMNPQEANYA
                     YLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGCAVLRYFSALEEGINLLP
                     GFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            1992..3311
                     /gene="potE"
                     /locus_tag="OQM87_001144"
     CDS             1992..3311
                     /gene="potE"
                     /locus_tag="OQM87_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCS1507365.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSITMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3367..5007)
                     /gene="pgm"
                     /locus_tag="OQM87_001145"
     CDS             complement(3367..5007)
                     /gene="pgm"
                     /locus_tag="OQM87_001145"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCS1507366.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5033..5578)
                     /gene="seqA"
                     /locus_tag="OQM87_001146"
     CDS             complement(5033..5578)
                     /gene="seqA"
                     /locus_tag="OQM87_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCS1507367.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5763..6527
                     /gene="ybfF"
                     /locus_tag="OQM87_001147"
     CDS             5763..6527
                     /gene="ybfF"
                     /locus_tag="OQM87_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1507368.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6667..6960
                     /gene="ybfE"
                     /locus_tag="OQM87_001148"
     CDS             6667..6960
                     /gene="ybfE"
                     /locus_tag="OQM87_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCS1507369.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7100..7630
                     /gene="fldA"
                     /locus_tag="OQM87_001149"
     CDS             7100..7630
                     /gene="fldA"
                     /locus_tag="OQM87_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCS1507370.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7840..7926
                     /gene="uof"
                     /locus_tag="OQM87_001150"
     CDS             7840..7926
                     /gene="uof"
                     /locus_tag="OQM87_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCS1507371.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7919..8365
                     /gene="fur"
                     /locus_tag="OQM87_001151"
     CDS             7919..8365
                     /gene="fur"
                     /locus_tag="OQM87_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCS1507372.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8449..8775)
                     /locus_tag="OQM87_001152"
     CDS             complement(8449..8775)
                     /locus_tag="OQM87_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507373.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8812..10218)
                     /gene="chiP"
                     /locus_tag="OQM87_001153"
     CDS             complement(8812..10218)
                     /gene="chiP"
                     /locus_tag="OQM87_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCS1507374.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLFHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10797..12461)
                     /gene="glnS"
                     /locus_tag="OQM87_001154"
     CDS             complement(10797..12461)
                     /gene="glnS"
                     /locus_tag="OQM87_001154"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCS1507375.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12664..14610)
                     /gene="nagE"
                     /locus_tag="OQM87_001155"
     CDS             complement(12664..14610)
                     /gene="nagE"
                     /locus_tag="OQM87_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCS1507376.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14943..15743
                     /gene="nagB"
                     /locus_tag="OQM87_001156"
     CDS             14943..15743
                     /gene="nagB"
                     /locus_tag="OQM87_001156"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1507377.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15803..16951
                     /gene="nagA"
                     /locus_tag="OQM87_001157"
     CDS             15803..16951
                     /gene="nagA"
                     /locus_tag="OQM87_001157"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1507378.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            16960..18180
                     /gene="nagC"
                     /locus_tag="OQM87_001158"
                     /gene_synonym="nagR"
     CDS             16960..18180
                     /gene="nagC"
                     /locus_tag="OQM87_001158"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCS1507379.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18228..18970
                     /gene="nagD"
                     /locus_tag="OQM87_001159"
                     /pseudo
     CDS             18228..18970
                     /gene="nagD"
                     /locus_tag="OQM87_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            19367..21031
                     /gene="asnB"
                     /locus_tag="OQM87_001160"
     CDS             19367..21031
                     /gene="asnB"
                     /locus_tag="OQM87_001160"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCS1507380.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21411..21487
                     /locus_tag="OQM87_001161"
     tRNA            21411..21487
                     /locus_tag="OQM87_001161"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21445..21447,aa:Met,seq:cat)
     gene            21496..21580
                     /locus_tag="OQM87_001162"
     tRNA            21496..21580
                     /locus_tag="OQM87_001162"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21530..21532,aa:Leu,seq:tag)
     gene            21604..21678
                     /locus_tag="OQM87_001163"
     tRNA            21604..21678
                     /locus_tag="OQM87_001163"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21636..21638,aa:Gln,seq:ttg)
     gene            21713..21787
                     /locus_tag="OQM87_001164"
     tRNA            21713..21787
                     /locus_tag="OQM87_001164"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21745..21747,aa:Gln,seq:ttg)
     gene            21803..21879
                     /locus_tag="OQM87_001165"
     tRNA            21803..21879
                     /locus_tag="OQM87_001165"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21837..21839,aa:Met,seq:cat)
     gene            21928..22002
                     /locus_tag="OQM87_001166"
     tRNA            21928..22002
                     /locus_tag="OQM87_001166"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21960..21962,aa:Gln,seq:ctg)
     gene            22040..22114
                     /locus_tag="OQM87_001167"
     tRNA            22040..22114
                     /locus_tag="OQM87_001167"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22072..22074,aa:Gln,seq:ctg)
     gene            complement(22268..23443)
                     /gene="ubiF"
                     /locus_tag="OQM87_001168"
     CDS             complement(22268..23443)
                     /gene="ubiF"
                     /locus_tag="OQM87_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCS1507381.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23589..25013
                     /gene="miaB"
                     /locus_tag="OQM87_001169"
     CDS             23589..25013
                     /gene="miaB"
                     /locus_tag="OQM87_001169"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCS1507382.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25166..26206
                     /locus_tag="OQM87_001170"
     CDS             25166..26206
                     /locus_tag="OQM87_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCS1507383.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAREEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26203..26670
                     /gene="ybeY"
                     /locus_tag="OQM87_001171"
     CDS             26203..26670
                     /gene="ybeY"
                     /locus_tag="OQM87_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCS1507384.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            26760..27638
                     /gene="corC"
                     /locus_tag="OQM87_001172"
     CDS             26760..27638
                     /gene="corC"
                     /locus_tag="OQM87_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCS1507385.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27663..29201
                     /gene="lnt"
                     /locus_tag="OQM87_001173"
     CDS             27663..29201
                     /gene="lnt"
                     /locus_tag="OQM87_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCS1507386.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            29598..30505
                     /gene="gltI"
                     /locus_tag="OQM87_001174"
                     /pseudo
     CDS             29598..30505
                     /gene="gltI"
                     /locus_tag="OQM87_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            30675..31415
                     /gene="gltJ"
                     /locus_tag="OQM87_001175"
                     /pseudo
     CDS             30675..31415
                     /gene="gltJ"
                     /locus_tag="OQM87_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015698156.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
     gene            31415..32089
                     /gene="gltK"
                     /locus_tag="OQM87_001176"
     CDS             31415..32089
                     /gene="gltK"
                     /locus_tag="OQM87_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCS1507387.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFMQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            32089..32814
                     /gene="gltL"
                     /locus_tag="OQM87_001177"
     CDS             32089..32814
                     /gene="gltL"
                     /locus_tag="OQM87_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCS1507388.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            32932..33867
                     /gene="rihA"
                     /locus_tag="OQM87_001178"
     CDS             32932..33867
                     /gene="rihA"
                     /locus_tag="OQM87_001178"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCS1507389.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            33951..35621
                     /gene="hscC"
                     /locus_tag="OQM87_001179"
     CDS             33951..35621
                     /gene="hscC"
                     /locus_tag="OQM87_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCS1507390.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(35681..37132)
                     /gene="djlC"
                     /locus_tag="OQM87_001180"
     CDS             complement(35681..37132)
                     /gene="djlC"
                     /locus_tag="OQM87_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCS1507391.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(37129..>37715)
                     /locus_tag="OQM87_001181"
     CDS             complement(37129..>37715)
                     /locus_tag="OQM87_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1507392.1"
                     /translation="SSSWGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWK
                     ALIAGGSERGLIYTQFVAQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWL
                     QSRVYARAHHYYHSWMHYFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFE
                     ELAAGSDRFYATLPWQPLTVQPECPVTLKDVSDL"
BASE COUNT         9194 a         9675 c         9575 g         9271 t
ORIGIN      
        1 cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg cgaatcacgg
       61 aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac gcaactgcgc
      121 ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc ttttgactgc
      181 ccgggtcatc agggaggtga gtttttccgt cgtcatccgg cagggaatca gttcgtggag
      241 tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc aatgggcgat
      301 ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa agtgtttaat
      361 gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt ggttttaaac
      421 gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa atctaaccac
      481 cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc acgtaacccg
      541 tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct gcgtgagctg
      601 atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct cgctgtgatt
      661 cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga taaaattggt
      721 catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca atttatcccg
      781 atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc gggtattcta
      841 gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca aatccataaa
      901 aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat gaacaacgcc
      961 tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact ggatatcaac
     1021 gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt ggtaaatggc
     1081 attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt cgtacctgaa
     1141 ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt tgatctgcgc
     1201 ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc agagaatcaa
     1261 tactttgtcg atccttacaa actgttgctg acaaccccag gtattgatgc acgtaacggc
     1321 gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg cgaaaatggc
     1381 gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc ggcagaagat
     1441 atggccaaac ttcagcaact tgttgccctg ctggtacgct tcgaaaaact gcttgagtcc
     1501 gacgcgccat tagcagaagt gctgccttcc atctacaaac agcatgaaga gcgctacgcc
     1561 ggttataccc tgcgtcagtt gtgccaggaa atgcatgatt tgtatgcccg ccacaacgtg
     1621 aaacaactgc aaaaagagat gttccgcaaa gcgcacttcc cacacgtcag catgaatccg
     1681 caagaagcca actacgccta tttacgcggt gaagtggagc tggttcgtct gccggatgcc
     1741 gaaggtcgta tcgctgccga aggggcgctt ccttatcctc cgggtgtgct gtgtgttgtt
     1801 ccgggtgaaa tctggggttg tgctgttttg cgttacttca gcgctctgga agaagggatc
     1861 aacctgctgc caggttttgc accggagctg cagggtgtct atatcgaaga acatgatggt
     1921 cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac cctgttgaaa
     1981 ggggaaaaat tatgagtcag gctaaatcga acaaaatggg cgtcgttcag ttaaccatac
     2041 tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag cttgccgaag
     2101 ttgggacaat ttcaattatc tcctggctgg tgacagccgt aggctcaatg gcactggcat
     2161 gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg tggtatgggc ggttatgccg
     2221 agtatgcttt cggtaaatcc ggtaacttta tggcgaacta tacctacggc gtctcactgc
     2281 tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa ctgctcggcg
     2341 cgagtttgtc gccagtgcag attggtcttg cgaccatcgg ggtgcagtgg atttgtaccg
     2401 tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattacc gtgtgggggg
     2461 tcattattcc ggtggtaggc ctgtgcatta tcggctggtt ctggtttagc ccgacgcttt
     2521 acgttgattc ctggaatccg catcatgcac cgttcttcag tgcagtaggt tcttccatca
     2581 ctatgacgct gtgggctttt cttggtctgg agtctgcgtg tgcgaatact gatgtagtgg
     2641 aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg tacgttaggt gcggcggtga
     2701 tttatatcgt ctccaccaac gtgattgcag ggattgtgcc taatatggag ctggcaaatt
     2761 caacggcacc atttggcctg gccttcgcac agatgttcac accagaagtg ggtaaagtca
     2821 ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag ttcaccattg
     2881 cccaggtgtt taaatcttca tctgatgaag gctacttccc taaaattttc tcccgtgtaa
     2941 ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt gattatccag agcgggttgc
     3001 cgctgatgac gattagcccg tcgctaaaca gtcagttcaa cgtgctggtt aacctggccg
     3061 tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt attcagaagg
     3121 tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt gttggcgcga
     3181 tgtatagctt ttatgcgctg tactcatccg gggaagaagc catgctgtac ggttccatcg
     3241 tgaccttcct tggttggaca ctgtatggtc tggtatcacc acgctttgaa ctgaaaaata
     3301 aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc gaccgccctt tttttattaa
     3361 atgtgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt tctcaatctg
     3421 cttgcgatgt tcttcaccga ggaagctttc gcagtagatc ttatatgcgt cttccgtgcc
     3481 tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc caatagaagc
     3541 accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg tcacctgcca gggtgctggc
     3601 gctcaccatt tccggagaca gcttagacag cgccgctttt tgtgcggaag tcgcagctgc
     3661 ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt agtgttcctg
     3721 cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa tgatgccgtc
     3781 cttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg cgctctcttc
     3841 gccgccaaag ccgaagctgc cgtcgaacag accatcaaca aaccatttga aacctaccgg
     3901 tacttctacc agcttacggc ccaagtcatt gaccacacgg tcgatcatcg cagaagaaac
     3961 cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga acaggtagtt
     4021 gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga cgataccgtg
     4081 acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca gtgccagcag
     4141 acccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt tatcgaggtg
     4201 cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga ggttgtaata
     4261 ctcgccaatg cgcttccagt attcgatacc ggaaccgccc aacggatcaa cgcccagcgt
     4321 caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt ccacgaacgg
     4381 ctgcaccaga tcctgctctt tcacatagcc ggatgccatc gcttcgtcga gggagatacg
     4441 cttcacgcct ttcaggccat cggccatcag tgcgttggcc ctgtcttcca ccactttggt
     4501 gacgttagta tcagccgggc caccatttgg cggattgtac ttgataccac catcttccgg
     4561 cgggttatgg gacggtgtaa tcacgatacc gtctgccagc gggccacctt ttttattgtg
     4621 aaccaggatg gcattggaaa cggcaggcgt cggagtgaag ccattgtttt cctgcacaat
     4681 gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag gctcggacag
     4741 ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg cacgttcttc
     4801 agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc gcgctgcact
     4861 gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg cttctggttt
     4921 cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct gttgtgcagg
     4981 ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg ttttagatag
     5041 ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg atgtgttcga
     5101 tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc gtgcctggca
     5161 catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct gccgcaaagt
     5221 acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg gcgtcaagag
     5281 aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc tctgcgtatt
     5341 cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt ttgaccggct
     5401 tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga gcagcaggct
     5461 gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc tcgccgatat
     5521 gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc gttttcatct
     5581 taatccagtg cagtgtctta gctgaataca agtcgccaag agtatatgaa taaatcccgg
     5641 cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta tttgttgcag
     5701 ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa aagaactttg
     5761 ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca gcacaataat tctcccatcg
     5821 ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg cgtactggct cgcgatctgg
     5881 taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca ccaagagagc
     5941 cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat gcactgcaga
     6001 tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg gcacttactg
     6061 cactggcccc cgatcgcatc gataaactgg tggcgatcga tatcgcaccg gtcgactatc
     6121 acgtacgccg tcatgatgag attttcgcag ctatcaacgc ggtcagtgaa tcggacgcac
     6181 aaactcgcca gcaagcagca gcaataatgc gccagcatct taatgaagaa ggggtgattc
     6241 agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtaccg gtattgtggg
     6301 atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcac cctgccctgt
     6361 ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat ttactggcac
     6421 aatttccaca ggcacgggcg catgtgattg caggcgcggg tcactgggtc catgctgaaa
     6481 aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc aactgattaa
     6541 aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg cgtgagtcat cgcgctgatg
     6601 tattatggcg cgctatccat ccgggcagag gcctggctga ttgtttcccc cgaagtcacc
     6661 aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc gcacgagcgt
     6721 cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg aacagctgcg tattaataaa
     6781 cgcaaccagc taaaacgcga caaagtacgt ggccttaagc gtgtcgaact gaagctgaac
     6841 gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc gtaatatgag ccgtagcgaa
     6901 ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg catcgtttaa
     6961 atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc cgcaagactg
     7021 cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt tcaataagtt
     7081 tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg acaccggtaa
     7141 taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg ccgatgtcca
     7201 tgacattgca aaaagcagca aagaagatct ggaagcttac gacattctgc tgctgggcat
     7261 cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc cgactctcga
     7321 agagattgat ttcaatggta aactggttgc gctgtttggt tgtggtgacc aggaagatta
     7381 cgccgaatat ttctgcgacg cattgggcac catccgcgac atcattgaac cgcgcggtgc
     7441 aaccatcgtt ggtcactggc caactgcggg ctatcatttc gaagcatcaa aaggtctggc
     7501 agatgacgac cactttgtcg gtctggctat cgacgaagac cgtcagccgg aactgaccgc
     7561 tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg acgaaattct
     7621 taatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag ataatgccaa
     7681 tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat gtcccagtgt
     7741 tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac aatatttgcc
     7801 agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat tatctcaaga
     7861 gcaaattctg tcacttcttc taatgaagtg aaccgcttag taacaggaca gattccgcat
     7921 gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc ctcgtttaaa
     7981 aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag atttatacaa
     8041 acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg tactgaacca
     8101 gtttgacgac gctggtatcg tcacccgcca caattttgaa ggcggtaaat ccgtatttga
     8161 gctgacacag caacatcacc acgatcacct gatttgcctc gactgcggca aggttatcga
     8221 atttagtgat gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac atggcattcg
     8281 cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt gccgcgaaga
     8341 tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac ctgcgggttg
     8401 gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt atttgttatt
     8461 actgcgtatt tcctgccaga ccttatcaca atcggctttc accgcctgat catttccggt
     8521 ttgcgttgca cgcagacact gctgataatc cagtacgcgt acactttcct ggtcggcaaa
     8581 ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctggcagg cttcaatttt
     8641 ttctggcgaa ccttgcgcgg tgttgataca ggcgctgtat gcctctttca gacggctgtc
     8701 ttcctgtggt gcggttgatt gcgcacaagc taccagcccc gatgccatca tggcgatgag
     8761 aatcagtttt ttcattgaat gcggcaggca caaaacctgc cgcgtcgggc atcagaagat
     8821 ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga tgttaccgta
     8881 accgccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt gcagtttgaa
     8941 catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc tgtaggcaga
     9001 ctcttcaata gtccggtttt tgtcgttgta cgcatccgga ttgctctgcc aggtcgcagg
     9061 tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa gattccagtt
     9121 tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttagcgt tgaagtagga
     9181 acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg gggtcatacg
     9241 ttgcaggaag tatccctgct gactgtcagc cttaacccag gtgccttcga ggcgcaaatc
     9301 cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaagcgg tgccgtcata
     9361 aagatcgttg acgctgcgat cgtcaacttt atcgcgggta ccatagaact ggtagctggt
     9421 ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat attgatcgat
     9481 atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga agtcgtattt
     9541 cgcaccgatg gagtgcagat aatcaacttt ggtggtttta tcgttctgat aaaactcatc
     9601 catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga aactcaaggc
     9661 accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag tacccggcat
     9721 aaagctccag tgcggcgcta acagcgtttg accggttggc tgaatgtaac ctgccctcgc
     9781 ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac cgcttttgtc
     9841 gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt cgttcgggtg
     9901 ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat caaggccgaa
     9961 catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg agtgaaaaag
    10021 gttggttttg tatttgtcgc cgtcggtaac atctttacgg tcgcgttcac gctgccagta
    10081 atagataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag cctccggcat
    10141 tgccataaag cccgacattg ctgtaacacc ggcaatagcc agcgccagcg tactacgttt
    10201 gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc accaaaggtg
    10261 aacagcgaaa aaaaataaaa attaaaaaag tagcgttgag aaaagtctcg ctgactacag
    10321 gaacagacta tttttcgcga cgcgaattat caatctcttt ctgtaaccag aatgtaagaa
    10381 catgagcaac cgcacatatt ttgatgattt ttgttacaag gcttaagttt gcggttgaaa
    10441 ggcttgcaga agaataaaaa cgtgaaattc cagcaagatg aaagcggtta cattttattt
    10501 aattatgttt tgtgatttta aatttatttt atggataaat tgcgaagcgg aattaattcg
    10561 cgaagaaaag cagaaaaaac gccgcagagc gcggcgtttg ggattgtttg ctatttcttg
    10621 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca cgcaaattca atatattgca
    10681 gagatcatgt aggcctaata agcgaagcgc atcaggcaat ttagcgtttg aaatttattg
    10741 tctatttatt gccggatgcg acgtaaatgc ctcacccggc ttggtagaac ttaaaattac
    10801 tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac cggtttttcg
    10861 gccgtggaat ggcggctgtc gaggcagaag taaccttcac gctcaaactg gaatgcttta
    10921 cccgctaccg catctttcag cgacggttca gcaaagccct gtttgatcac cagcgattcc
    10981 gggttaatca ccgacaggaa atcatccgca gcacctgggt taggcacgct gaacagacga
    11041 tcatacaaac ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg aataacacct
    11101 ttgactttac gaccatctgc cggatctttg cttaaggtgt cggcgtcata agtacagaag
    11161 atggtggtga tattaccttc ggcatctttc tccacgcgtt cagccttaat gacgtaagca
    11221 ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt agcttcttcg
    11281 cggaaatcgg cgcgatcgat ccaaatttca ccgctaaacg gcacctgacg gctccccatt
    11341 tccggtttgt tcggatggtt cggcatggtg accatttcgc cttcgccctg atagttttcg
    11401 ataaccagtt tcaccggatc gataactgcc attgcgcgcg gcgcattttc gttgagatct
    11461 tcacggatgc aggattccag cgacgccatc tcaatggtgt tgtcctgctt ggtcacgccg
    11521 atgcgtttgc agaactcacg aatagaagcc gcagtgtaac cacgacgacg cagaccggaa
    11581 atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac cagcaggttc
    11641 agcttacgct tggacatcac agtgtattcc agattcaggc gcgagaattc gtactggcgc
    11701 ggatgaacag gaatggtgat gttatccagt acccagtcgt acagacgacg gttgtcctgg
    11761 aactcaagcg tacacagaga gtgcgtaata ccttccagcg catcgctgat gcaatgggtg
    11821 aagtcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc agcaaattta
    11881 atgcggtaca gcaccggatc gcgcattacg ataaatggcg acgccatgtc gattttcgca
    11941 cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa cagcgccaag
    12001 ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac ccggctgcgt cagggtaccg
    12061 cggtattcgc ggatctgttc cggtgtcagt tcatcaacgt acgccaggcc tttattgatc
    12121 agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg gacgttacca
    12181 gaccagtgaa aacctaacca ctctacgtcg tttttgatcg aatcgacata ctcgatatct
    12241 tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata gtcctgggcg
    12301 atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc attcggctcc
    12361 ggcgggaaac gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc atcgatgatc
    12421 tgacggataa agttagtcgg gcgggcttct gcctcactca tcgtggattc ctcaaagcgt
    12481 aaacaacgta taacggcgta tgatcttata agcgggacag gctgacaact gttagttttt
    12541 taaaattgat aatctaacaa ggattatggg gatgattgat gaaaaaaaaa gcggcagaga
    12601 agatctccac cgcttgtcat tgttggatgc gacgctgccg tgtcgcatca ggcataaagc
    12661 agattacttt ttgatttcat acagcggtgt ttgacccgcc acaacatggc cctgagcttt
    12721 aatgatcaag ccactgaaat cgtcgatatt gctgcaaacc accgggctaa tcatcgagcg
    12781 ggcgttagcg ttcaggtaat ccagatccat ttccagaatc ggttgccctg cgcttacctg
    12841 cgcaccctct tccaccagac gtttaaagcc tttaccttcc agcgctacgg tgtcgatacc
    12901 catatggacg acgatctccg cgcctttttc ggtttccagg cagaacgcgt ggttggtgtt
    12961 gaagattttc acgattgtcc ctgcggctgg tgatacgacg attttatctg tcggtttcac
    13021 cgccacaccg tcacccaccg ctttgctggc gaatgcttcg tcaggaacct gatccagtgc
    13081 cacgacatca ccggtaatcg gcgataccag ctccgcgata gataccgcgt ttggtacagc
    13141 ctgcggtttt gctacaggcg cggcagttgc cggagttgct tcagctgacg cagcggctac
    13201 cggaccacgg gcaacgactt tcttcatcgc atcgccgatg gattctgctt tcgcgccaac
    13261 aatcacctga atagtctgtt tgttcagttt cactacacca gaagcaccca gacgtttaca
    13321 catcgtatcg ttgacgcggg cagagtcagc cacggtcagg cgcagacggg taatacaggc
    13381 atcaatcgct ttcaggttgt cagtaccgcc aaccgcagca atatagttgg ttgccagttg
    13441 agtcagacct tcttcagtat tgctgttggc ttcttcagta acgatctcgt cttctttatc
    13501 ttcacgaccc ggcgttttca ggttgaacat gcggataacc aaactgaaca ccacgaagta
    13561 gatagcgaag aagataacgc ccatcaccag cagcatccag acgttctggc tggcggccgg
    13621 caggttatac atcaacgcgt agtcgatagc acccgcagag aaagagaagc cggcatggat
    13681 acccagcagc gttgccacaa acaggctgat accggtcagc agtgcgtgca ggaggtacag
    13741 cagcggcgca aggaacatga acaggaattc cagcggctca gtcacaccgg tcaggaacgc
    13801 agtaacagca acagaaagca gcatcccgcc aaccatcgga cgacgctctt tcggtgctgc
    13861 gaagtacatc gccagcgccg cacccggcag accgaacatc atgatcggga agaagccgga
    13921 catgaacatc cccgcggtgc cgtcaccagc gtagaaacgg ttgatgtcgc cgtggaaaac
    13981 cgtacccgcc gcgttggtga attcaccaat ctggaaccag gcgatggtgt tcagcacctg
    14041 atgcagaccg gttgggatca gcagacggtt gatgaaacca aagataccgg aacccagcgc
    14101 gcccgcagaa acgatccact cgccgcctgc atggatagcg tgctgtaccg gcggccagac
    14161 gtaaccaaaa atggccgcca gcaccagaca gaagaagccg gtggcgatcg gcacaaagcg
    14221 tttgccgccg aagaagctca ggaagtccgg cagtttaata tcggaccaac ggttataggc
    14281 tgcgccacca accagaccgg taatgatacc cgccagtaca cccatgttaa tttctgggtt
    14341 gatggtcacc atcgctttgg ttaacacaaa gtaacctacc gcacccgcca gcgccgccgc
    14401 acctgcgctg tctttcgacc agctggatgc cacaccgatg gcgaagatta atgcgaggtt
    14461 atcaaaaatt gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat ctggctgacc
    14521 gaatcgcagc aacagcgccg ccaccggcag caccgcgata gggagctgta acgccctacc
    14581 gagtcgctgg aaaaaaccta aaatattcat cttattcccc ctacgagaac cctatttggc
    14641 tcgtttaaag ccgtattttt attttgctgc aaattttact gccgatgttc tgtaatcaga
    14701 ttgttagatc atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa attaaacgcg
    14761 tgtcttttgt gagttttgtc accaaatatc gttatcatca ctccctttta ctggctaaac
    14821 cagaaaactt attttatcat tcaaaaaatc aagtcggatt gacgcctgtc tgcgcaaatc
    14881 caggttacgc ttaaagatgt ctaatccgcc aacggcttac attttactta ttgaggtgaa
    14941 taatgagact gatccccctg actaccgctg aacaggtcgg caaatgggct gctcgccata
    15001 tcgtcaatcg tatcaatgcg ttcaaaccaa ctgccgatcg tccgtttgta ctgggcctgc
    15061 cgactggcgg cacgccgatg accacctata aagcgttagt cgaaatgcat aaagcaggcc
    15121 aggtcagctt taagcacgtt gtcaccttca acatggacga atatgtcggt ctgccgaaag
    15181 agcatccgga aagctactac agctttatgc accgtaattt cttcgatcac gttgatattc
    15241 cagcagaaaa catcaacctt ctcaacggca atgccccgga tatcgatgcc gagtgccgcc
    15301 agtatgaaga aaaaatccgt tcttacggaa aaattcatct gtttatgggc ggtgtaggta
    15361 acgacggtca tattgcattt aacgaaccgg cgtcttctct ggcttctcgt actcgtatca
    15421 aaaccctgac tcatgacact cgcgtcgcga actctcgttt ctttgataac gatgtcaatc
    15481 aggtgccaaa atatgccctg actgtcggtg ttggtacact gctggatgcc gaagaagtga
    15541 tgattctggt gctgggtagc cagaaagcac tggcgctgca ggccgccgtt gaaggttgcg
    15601 tgaaccatat gtggaccatc agctgtctgc aactgcatcc gaaagcgatc atggtgtgcg
    15661 atgaaccttc caccatggag ctgaaagtta agactttaag atatttcaat gaattagaag
    15721 cagaaaatat caaaggtctg taattgttat ccctgccctg gttccttgct cagggcaata
    15781 ttttttaaaa tcgggggtca gaatgtatgc attaacccag ggccggatct ttaccggcca
    15841 cgaatttctt gatgaccacg cggttgttat cgctgatggc ctgattaaaa gcgtctgtcc
    15901 ggtagcggaa ctgccgccag agatcgaaca acgttcactg aacggggcca ttctctcccc
    15961 cggttttatc gatgtgcagt taaacggctg cggcggcgta cagtttaacg acaccgctga
    16021 agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat caggctgtac
    16081 taactatctg ccgacgctta tcaccaccag cgatgagctg atgaaacagg gcgtgcgcgt
    16141 tatgcgcgag tacctggcaa aacatccgaa tcaggcgtta ggtctgcatc tggaaggtcc
    16201 gtggctgaat ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta agcctgatgc
    16261 cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga ccctggcacc
    16321 ggaaatggtt cctgcggaag tcatcagcaa actggcaaat gccgggattg tggtttctgc
    16381 cggtcactcc aacgcgacgt tgaaagaagc aaaagccggt ttccgcgcgg ggattacctt
    16441 tgccacccat ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg gcctggcggg
    16501 cgcgatcctc gacgaagctg acatttattg cggtattatc gctgatggcc tgcatgttga
    16561 ttacgccaac attcgtaacg ctaaacgcct taaaggcgac aaactgtgtc tggttaccga
    16621 cgccaccgcg ccagcaggtg ccaacattga acagttcatt tttgcgggta aaacaatata
    16681 ctaccgtaac ggactttgtg tggatgagaa cggtacgtta agcggttcat ccttaaccat
    16741 gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg aagtgctgcg
    16801 tatggcgacg ctctatccgg cgcgtgcgat tgacgttgaa aaacgtctcg gcacgctcgc
    16861 cgcaggtaaa gtagccaacc tgaccgcatt cacacctgat tttaaaatca ccaagaccat
    16921 cgttaacggt aacgaggtcg taactcaata agagaaagta tgacaccagg cggacaagct
    16981 cagataggta atgttgatct cgtaaaacag cttaacagcg cggcggttta tcgcctgatt
    17041 gaccagtacg ggccaatctc gcggattcag attgccgagc aaagccagct tgcccccgcc
    17101 agcgtaacca aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga agttgatcag
    17161 caggcctcca ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg caatttccac
    17221 gcaatcggcg tacggcttgg tcgtcatgac gccaccatca ctctgtttga tctcagcagc
    17281 aaagtgctgg cagaagaaca ttacccgctg ccggaacgta cccagcagac gctggaacat
    17341 gccctgctga atgccattgc tcagtttatt gatagctacc agcgcaagct gcgcgagcta
    17401 atcgccattt cggtgatcct gccagggctt gttgacccgg acagcggcaa aattcattac
    17461 atgccgcata ttcaggtaga aaactggggg ctggtagaag cactgaaaga acgttttaaa
    17521 gtgacctgtt tcgttggtca cgatatccgt agtctggcgc tggcggagca ctacttcggt
    17581 gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac cggggccggg
    17641 attatctcta acgggcgcat ttttattggc cgcaacggta acgtcggtga aattggccat
    17701 attcaggtcg aaccgctggg tgaacgctgc cactgcggca actttggctg cctggaaact
    17761 atcgccgcca acgctgccat tgaacaacgg gtgttgaatc tgttaaagca gggctaccag
    17821 agccgtgtgc cactggacga ctgcaccatc aaaactatct gcaaagccgc gaacaaaggc
    17881 gatagcctgg cctcggaagt gattgagtat gtcggtcgtc atctgggtaa aaccatcgcc
    17941 attgctatca acttatttaa tccgcaaaaa attgttattg ctggtgaaat caccgaagcc
    18001 gataaagtgc tgctccctgc tattgaaagc tgcattaata cccaagcgct gaaggcgttt
    18061 cgtactaatc tgccggtggt acgttctgag ctggatcacc gctcggcaat cggcgctttt
    18121 gcgctggtaa aacgcgccat gctcaacggt attttgctcc agcatttgct ggaaaattaa
    18181 tgtgctttta tagtggcgct tattgttgtc aatattctgg gtagtccatg accattaaaa
    18241 atgtaatttg cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta ccgggtgcag
    18301 cggaattttt gcacgggatt atggataaag gcctgccgct ggtgttgctg accaactatc
    18361 cttcgcagac tgggcaagat ctggcgaacc gctttgccac tgccggtgtc gatgtgccgg
    18421 atagcgtgtt ttatacctct gcgatggcga ccgccgattt cctgcgtcgc caggaaggca
    18481 agaaagcgta tgtggtgggc gaaggcgcac tgattcatga actgtacaaa gccggtttca
    18541 ctattaccga tgtgaaccct gatttcgtga ttgttggcga aacacgttcc tacaactggg
    18601 acatgatgca taaagcagcc tatttcgtcg ctaacggtgc acgctttatc gccaccaacc
    18661 cggacacggt ttttatcccg cttgtggcgc gttgtgtgca gggattgaga aaatctccgg
    18721 gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc atccgcgcgg cattaaacaa
    18781 aatgcaggcg cattcggaag aaacagtgat tgtcggcgat aacctgcgta ccgatattct
    18841 ggcgggcttc caggccggtc tggagacgat tctggtgctt tctggtgttt catcgctcga
    18901 cgatatcgac agtatgccgt tccgcccaag ctggatttac ccgtcagtcg ctgaaatcga
    18961 cgttatctga ataccaccca cgtctgcgga cgtggttggc tctgatgaca aacgccaaac
    19021 tgcctgatgc gctacgctta tcaggcctac gcagctcctg caatatattg aattttcatg
    19081 cttttgtagg ccgtataagg cgttcacgcc gcatccggca tgaacaaatc gcactttatc
    19141 agcaatttgg cccccgtaaa atataaccat tcaataaaac ccgctaaaaa ttatcgattc
    19201 atcaataaat acatcctcac accagaccct ttttcaccaa tcgacaatca ccattacgtt
    19261 ttttattttt gcactgccaa atagcttgcg cacagtgctg ctttgcggca tttttttaaa
    19321 caagcaaaca caacaagcaa caaataccag gttaacggag aaggttatgt gttcaatttt
    19381 tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt aagaaagcac tcgagctgtc
    19441 acgcctgatg cgtcatcgtg gcccggactg gtccggtatt tatgccagcg ataacgccat
    19501 tctcgctcac gaacgtctgt caattgttga cgttaacgcg ggggcgcaac ctctctacaa
    19561 ccaacaaaaa acccacgtac tggcggtaaa cggtgaaatc tacaaccacc aggcattgcg
    19621 cgctgaatat ggcgatcgtt atcagttcca gacagggtct gactgtgaag tgatcctcgc
    19681 gctgtatcag gaaaaagggc cggaatttct cgacgacttg cagggcatgt ttgcctttgc
    19741 cctgtacgac agcgaaaaag atgcctacct gattggtcgc gaccatctgg ggatcatccc
    19801 actgtatatg gggtatgacg aacacggtca gctgtatgtg gcctcagaaa tgaaatcgct
    19861 ggtgccagtt tgccgcacga ttaaagagtt cccggcgggg agctatttgt ggagtcagga
    19921 cggcgaaatc cgttcttatt atcatcgcga ctggttcgac tttgatgcag tgaaagataa
    19981 cgtgaccgac aaaaacgagc tgcgtcaggc actggaagat tccgttaaaa gccatctgat
    20041 gtctgatgtg ccttacggtg tgctactttc tggtggtctg gattcctcaa ttatttccgc
    20101 tatcaccaag aaatacgcag cccgtcgcgt ggaagatcag gaacgctctg aagcctggtg
    20161 gccgcagtta cactcctttg ctgtaggtct gccgggttca ccggatctta aagcagccca
    20221 ggaagtggca aaccatctgg gcacggtgca tcacgaaatt cacttcactg tacaggaagg
    20281 tctggatgcc atccgcgacg tgatttacca catcgaaact tatgatgtga ccactattcg
    20341 cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag gcgatgggca ttaaaatggt
    20401 gctgtccggt gaaggttctg atgaagtgtt tggcggttat ctttacttcc ataaagcgcc
    20461 caacgctaaa gaactgcatg aagagacggt gcgtaaactg ctggccctgc atatgtatga
    20521 ctgcgcgcgc gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg ttccgttcct
    20581 cgacaaaaaa ttccttgatg tggcgatgcg cattaacccg caggataaaa tgtgcggtaa
    20641 cggcaaaatg gaaaaacaca tcctgcgtga atgttttgag tcatacctgc ccgcaagcgt
    20701 ggcctggcgg cagaaagagc agttctccga tggcgtcggt tacagttgga tcgacaccct
    20761 gaaagaagtg gcggcgcagc aggtttctga tcagcaactg gaaactgccc gcttccgctt
    20821 cccgtacaac acgccgacct caaaagaagc gtatctgtac cgcgagatct ttgaagaact
    20881 gttcccactt ccgagtgccg ctgagtgcgt gccgggcggt ccttccgtcg cgtgttcttc
    20941 agctaaagcg atcgaatggg atgaagcgtt caaaaaaatg gacgatccgt ctggtcgtgc
    21001 ggttggcgtt caccagtcgg cgtataagta agaaatgcgg tactgccccg gagaaatccg
    21061 gggctgtttt agttattgcg ctgcttgttg attctgatat gcagtaagga aatgttccgg
    21121 gtttgccaca tcactgtttg acaactcatc actgtaggcc agtatttcat agattaattt
    21181 cccaccacta ttgttgaatt ccgaatttca tcctgtacga caaaagaatg gctgtagggt
    21241 ggggaaaaaa cacaaagtaa acaataattg acgaatatag cgccacgctg ttcgcaacct
    21301 aaccaaacag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct gcaaggctct
    21361 atacgcataa tgcgccccgc aacgcagata aggtatcgcg aaaaaaagat ggctacgtag
    21421 ctcagttggt tagagcacat cactcataat gatggggtca caggttcgaa tcccgtcgta
    21481 gccaccatct tttttgcggg agtggcgaaa ttggtagacg caccagattt aggttctggc
    21541 gccgcaaggt gtgcgagttc aagtctcgcc tcccgcacca ttcaccagaa agcgttgtac
    21601 ggatggggta tcgccaagcg gtaaggcacc ggtttttgat accggcattc cctggttcga
    21661 atccaggtac cccagccatc ttcttcgagt aagcggttca ccgcccggtt attggggtat
    21721 cgccaagcgg taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc
    21781 ccagccatcg aagaaataat ctggctacgt agctcagttg gttagagcac atcactcata
    21841 atgatggggt cacaggttcg aatcccgtcg tagccaccaa attctgaatg tgtcgaatac
    21901 gttcggcaaa ttcaaaaacc aatttgttgg ggtatcgcca agcggtaagg caccggattc
    21961 tgattccggc attccgaggt tcgaatcctc gtaccccagc caatttattc aagacgctta
    22021 catcgtaagt gcacccagtt ggggtatcgc caagcggtaa ggcaccggat tctgattccg
    22081 gcattccgag gttcgaatcc tcgtacccca gccacattaa aaaagctcgc ttcggcgagc
    22141 tttttgcttt tctgcgttca ttcaatgtcg aatgcgatgt tgacacgtct tatccttcaa
    22201 tgtcggatgc gacgctgccg cgtcttatcc gacctacggt tggcacgcat ccggcaatgt
    22261 tgtaaggcta caaccctaac gcatatttca gcgcctgacg tttcaacacg ccagcacgct
    22321 ctgccgccat taacccgaga ttacgcacaa aacgcagcgg cggcagatta ttactgaatc
    22381 cggcataaaa cagatccata ccactttgca taatgaagtt atccgccatg cgccgcatct
    22441 ggtaacgctt gaggacagga taactggccc acgcttcacc gtagctgcgg gcattcacca
    22501 gaacatcaat cagggcatcg acatcacgat aaccaagatt caccccctgc cccgccagcg
    22561 gatggatggt atgcgcggca tcgcccacca gcgcaagccc cggctgcaca tactgcaacg
    22621 catgtcggcg cgtcagcgga aacgcaccag cggcaagcgg tgtaacgtaa cccagacgcg
    22681 acgggaaatg cttcgcgatt tccgcctgga gttgcgccat attcatattc tgcaactggc
    22741 gaatacgcgc cggagagtca taccacacca gcgatgccca gttatcaaac aacggcagaa
    22801 acgcacgcgg tccgtccggg gtaaattgct gccaggtgct gtcgccggga tcgttctcac
    22861 actgtacgct aatcaacatg cacgactgcg catactgcca ggcatgaacg ccaattcccg
    22921 ccatctgccg cacctgagaa tttgcgccgt cggcaccaat caccagtttc gcgcggatcg
    22981 tttcaccgcc cttcagttcc agctcctgaa gatcattatc tcgatgtaat gcaatcagcg
    23041 aggttggcac acgtaacgtt actttcggat gcgcttccag cgcctgccac aacgcctgtt
    23101 gcaggactgt gttttccacc atgtagccaa gcaacggcaa ctttaattcg gctgcgtcaa
    23161 ataccacatg tgccgtttcc cactcccagg tttccagtct gcggtaagga tggcaacgca
    23221 tagcctgtac tgcatcccag acccctaccc ctttaagcaa tgataccgaa gccgcgctga
    23281 tcgccgagat ccgcacgtcc ggttggctgt cagcgacaaa cggtgcaggt tctgcgtgct
    23341 cgatcaccgt taccgaaaat ccgtgctgtg ccagccccag cgccagtgcg ccgccgacca
    23401 ttcctccgcc gacaatggca atttccgttg gttgatttgt catggtcaat catcctgtta
    23461 gcaatatgct ttaagtttac aggattttta cccccaaggg ctgataacgc tcatactggt
    23521 cacaacggca gcaaagcatt acactatgcg ccctgcattc ctggctacta tttcgcaaga
    23581 gcaagtcgat gaccaaaaaa ctccatatta aaacctgggg ctgtcagatg aacgagtacg
    23641 attcatcgaa gatggccgat ctgctggatg ccacccacgg ctatcaactg accgacgtgg
    23701 cggaagaagc ggatgtgcta ctgctgaaca cctgctcaat ccgcgagaag gctcaggaaa
    23761 aagtcttcca tcagttgggt cgctggaaac tgttaaaaga gaagaatcca gacctgatta
    23821 tcggcgtcgg tggctgcgtg gcatcgcaag aaggcgagca cattcgccag cgcgcccact
    23881 atgtcgatat tatttttggg ccgcaaacgc tgcaccgtct gccggagatg atcaactccg
    23941 tgcgcggcga ccgcagcccg gttgtagata tcagcttccc ggaaatcgag aagtttgacc
    24001 gtctgccgga accgcgcgcc gaagggccga ccgcgtttgt ctccatcatg gaaggctgca
    24061 ataaatattg cacctactgc gtggtgcctt acacccgtgg tgaagaggta agccgtccgt
    24121 ctgacgatat tctgtttgag attgcccagc ttgcggctca gggcgtgcgt gaagtcaacc
    24181 tgctcggtca gaatgtgaat gcctggcgtg gtgagaacta cgacggcacc accggatcgt
    24241 ttgccgatct gttgcgtctg gttgctgcga tcgacgggat cgatcgtatt cgctttacca
    24301 ccagccatcc gatcgaattc accgacgata tcatcgaagt gtaccgcgac acgcctgagc
    24361 tggtgagctt cctgcatctg ccggtacaga gcggttccga tcgcattctg aacctgatgg
    24421 gacgtaccca tacggcgctg gagtacaaag cgatcatccg taaactgcgt gcggcgcgtc
    24481 cggatattca gatcagttct gacttcattg ttggcttccc tggcgaaacc accgaagact
    24541 tcgagaaaac gatgaagctg attgccgacg tcaatttcga catgagctac agctttatct
    24601 tctctgcacg tccaggaacg cctgccgccg atatggttga tgatgttccg gaagaagaga
    24661 agaagcagcg tctgtatatt ctgcaagaac gtatcaatca gcaagcgatg gcatggagcc
    24721 gtcggatgct cggcaccacc cagcgtattc tggtagaagg gacttcgcgt aagagcatca
    24781 tggagctttc cgggcgtacc gaaaataacc gcgtggtcaa cttcgagggc acaccggata
    24841 tgatcggtaa attcgtcgat gtagaaatta ccgacgtcta cccgaactct ctgcgcggta
    24901 aagtggtgcg aactgaagat gaaatgggtc tgcgcatggc agaaacaccg gagtcggtga
    24961 ttgcccgcac ccgcaaagaa aacgaccttg gcgtgggtta ttatcagccg taattctcag
    25021 gcctgccgtt ccggtgggcc ttgttttttc tctctttatc cccatcttta tcgcaatgct
    25081 tgcgcctttt tcctgtggcg tgaataattt ccttataggc cggtgaatgt tcagcttccg
    25141 gcggcataca gataagagga acggtttgaa catagacact cgcgaaatca ccctggagcc
    25201 agcagacaat gcgcgtctgt tgagcctgtg cggcccgttt gatgacaaca tcaagcagct
    25261 cgaacgccgt ctcggcatcg agatcaatcg ccgcgataac cactttaaac tgaccggccg
    25321 tccgatttgc gtcaccgctg cggcagacat tctgcgtagc ctgtatgtcg atactgcccc
    25381 gatgcgcggt cagattcagg atatcgaacc ggaacagatc caccttgcga ttaaagaagc
    25441 gcgggtactg gagcaaagcg cggagagcgt gccggagtac ggcaaagcgg tcaatatcaa
    25501 aactaaacgc ggcgtaatta agccgcgcac gccaaaccag gcgcagtaca tcgccaatat
    25561 tctcgaccat gacattacct tcggcgttgg cccggcgggt acgggtaaaa cctacctggc
    25621 agtggctgcg gcagttgatg ccctggagcg tcaggaaatt cgccgtattc tgctgactcg
    25681 tccggcggtc gaagccggtg agaaactggg cttcctgcct ggcgatttaa gccagaaagt
    25741 agacccgtat ctgcgcccac tgtacgacgc gctgtttgaa atgctgggct ttgagaaagt
    25801 cgagaaactg attgagcgca acgttattga agtcgcgccg ctggcctata tgcgtggtcg
    25861 tacgctgaac gacgcgttta tcattctcga tgagagccag aacactacca tcgaacagat
    25921 gaagatgttc ctgacccgta tcggttttaa ctcaaaagcg gttatcaccg gcgacgtcac
    25981 acagatcgac ctgccgcgta atactaaatc aggcttacgt catgccattg aagtgctggc
    26041 cgatgtcgaa gagatcagct ttaacttctt ccacagcgaa gacgtggttc gtcacccggt
    26101 ggtggcgcgt atcgttaacg cttatgaagc cagggaagaa gcagaacaaa aacgtaaagc
    26161 ggcgctggca gcagaacgca agcgcgaaga acaggaacaa aaatgagtca ggtgatcctc
    26221 gatttacaac tggcatgtga agataattcc gggttaccgg aagagagcca gtttcaaaca
    26281 tggctgaatg cggtgatccc gcagtttcag gaagagtcgg aagtgactat tcgcgtggtc
    26341 gataccgccg aaagccacag tctgaatctg acctaccgcg gtaaggataa gccgaccaac
    26401 gtgctctcct tcccgtttga agtgccgcct ggcatggaga tgtcgctact gggcgatctg
    26461 gttatctgcc gtcaggtggt tgagaaggaa gctcaggagc aaggcaaacc actggaggcg
    26521 cactgggcgc atatggtggt gcacggcagt ctgcatttgt taggttacga tcacattgaa
    26581 gatgacgaag cagaagaaat ggaagccctc gaaacagaga ttatgcttgc tctgggctat
    26641 gaggatccgt acattgccga gaaagaataa tttgccaggc atttgactgg cagacgtccc
    26701 cgccgttggt gattaacgac ggcgttttga ttaactaaca tgagaaccca tacgacgcca
    26761 tgagcgacga caattcacac agtagtgaca cgataagcaa caagaaggga tttttctccc
    26821 tgttactcag ccaacttttc cacggtgaac cgaaaaaccg tgacgaactg ctggcgctga
    26881 tccgtgattc cgggcagaac gaccttatcg acgaagatac gcgcgatatg ctcgaagggg
    26941 tgatggacat cgcagaccaa cgcgttcgcg acatcatgat cccccgctcc cagatgatta
    27001 ccctgaaacg caaccagacg ctggacgaat gccttgatgt catcatcgag tccgcccact
    27061 cacgtttccc ggtgattagc gaagacaaag atcacattga agggattctg atggcgaaag
    27121 acttgctgcc gtttatgcgc agcgatgctg aagccttcgg catggacaaa gtgttacgtc
    27181 aggcggttgt cgttcctgaa agtaagcgcg tagaccggat gctgaaagag tttcgctctc
    27241 agcgttacca catggcgatc gttattgacg aattcggtgg ggtttccggt ctggtgacca
    27301 ttgaagacat cctggaactg attgttggtg agattgaaga cgagtatgac gaagaagatg
    27361 atatcgactt ccgtcagctg agtcgtcata cctggaccgt gcgcgcactg gcttccattg
    27421 aagacttcaa cgaagcgttc ggcacccact ttagcgatga agaagtcgac actatcggtg
    27481 gtctggtgat gcaggcgttt gggcatcttc cggcgcgtgg cgaaactatc gacatcgacg
    27541 gttaccagtt caaagtggcg atggccgata gtcggcgtat tattcaggtt catgtcaaaa
    27601 tcccggatga ctcaccccag ccaaagctgg atgaataaaa ccgaaactgg atagataact
    27661 acatggcttt tgcctcatta attgaacgcc agcgcattcg cctgctgctg gcgttattat
    27721 tcggtgcctg cggaacgctg gccttctctc cttacgacgt ctggcctgcg gcgattattt
    27781 cgctgatggg gcttcaggcg ttgaccttta accgtcgccc actccagtct gccgctattg
    27841 gcttttgctg gggatttggc ctctttggca gcggtattaa ctgggtctat gtcagcatcg
    27901 cgacctttgg cgggatgcct ggcccggtta acatcttcct agtggtgctg ctggcggcgt
    27961 atttgtcgct gtataccgga ctgtttgctg gcgtgctgtc gcgtctgtgg ccgaaaacca
    28021 cctggctgcg cgtggcgatt gccgcccctg ctctctggca agtgaccgag tttctgcgcg
    28081 gttgggtgct gactggcttc ccgtggttac agttcggcta tagccagatt gatggcccgt
    28141 taaaagggct ggcaccgcta atgggcgtgg aagccattaa cttcctgctg atgatggtta
    28201 gtggcctgct ggcactggcg ttggtcaaac gcaactggcg tccgctggtg gtggccgtcg
    28261 tgctgtttgc cctacccttc ccgctgcgtt acatccaatg gtttaccccg caaccggaga
    28321 aaaccattca ggtttcgatg gttcagggcg atattccgca atcgctgaaa tgggacgaag
    28381 gccagcttct taatacgcta aagatttact acaacgcaac ggcaccgctg atgggcaaat
    28441 catcgttgat tatctggccg gagtcggcga taaccgatct ggaaattaat cagcaaccgt
    28501 tcctcaaagc actggacggt gagttgcgtg ataaaggtag ctcgctggta accgggattg
    28561 tcgacgcgcg tctcaataag cagaaccgct acgataccta caacaccatc atcacgctgg
    28621 gtaaaggtgc accgtacagc tacgaatcag ccgatcgcta taacaaaaac catctggtgc
    28681 cgtttggcga gtttgtcccg ctggagtcga ttctgcgtcc gttagcaccg ttctttgatc
    28741 tgccgatgtc gtcgttcagc cgtgggccat atatccagcc gccgctgtcg gcaaatggta
    28801 ttgagcttac tgcggctatt tgctacgaga tcattctcgg cgagcaagtg cgcgataact
    28861 tccgcccgga taccgactac ctgctgacta tctccaacga tgcgtggttt ggtaaatcta
    28921 ttggtccatg gcaacacttc cagatggcgc gaatgcgtgc gctggagctg gcgcgcccac
    28981 tgttgcgcag caccaacaac ggcattacgg cggtgattgg cccgcagggt gagattcagg
    29041 cgatgatccc gcagttcacc cgcgaggtgt taaccactaa cgtgacgccg accaccggac
    29101 tcacaccata cgcacgtacc ggcaactggc cgctgtgggt gctgacggca ctgtttggtt
    29161 ttgctgctgt gttgatgagt ctgcgtcagc gacgtaaata atcccttctt atcgtgccgg
    29221 aatacatccc atatttcggc acgctcaccg gatgcggcat cagtgcatta tccggataac
    29281 aatattctcc tatccaactt tggcacatct attgctttgt tatacaaggc aaagcttgaa
    29341 ccagcatcag tacaacccgg ttgcaccaca gcaatccacc ggtagcacca aaagagtgca
    29401 ttttaagaat tttgcttcag aatggtgcgg tgcacctcgc aaaaacaaac aataaaacat
    29461 caatatcttt acatttagcc aaactaaatg ttacctaaca ggcacaacac tgcacaataa
    29521 agttgcagac gataacaaca caaacactca caacgggtat ccatgcgttc ttaacgcaga
    29581 agataaagga gttggatatg caattacgta aacctgccac agcaatcctc gccctggcgc
    29641 tttccgcagg actggcacag gcagatgacg ccgccccggc agcaggcagt acgctggaca
    29701 aaatcgccaa aaacggtgtg attgtcgtcg gtcaccgtga atcttcagta cctttctctt
    29761 attacgacaa tcagcaaaaa gtggtgggtt actcgcagga ttactccaac gccattgttg
    29821 aagcagtgaa aaagaaactc aacaaaccgg acttgcaggt aaaactgatt ccgattacct
    29881 cacaaaccgt attccactgc tgcaaaacgg cactttcgat tttgaatgtg gttctaccac
    29941 caacaacgtc gaacgccaaa aataggcggc tttctctgac actattttcg tggtcggtac
    30001 gcgcctgttg accaaaaagg gcggcgatat caaagatttt gccgacctga aaggcaaagc
    30061 cgtagtcgtc acttccggca ctacctctga agttttgctc aacaaactga atgaagagca
    30121 aaaaatgaat atgcgcatca tcagcgccaa agatcacggt gactctttcc gcaccctgga
    30181 aagcggtcgt gccgttgcct ttatgatgga tgacgctctg ctggccggtg aacgtgcgaa
    30241 agcgaagaaa ccagacaact gggatatcgt cggcaagccg cagtctcagg aagcctacgg
    30301 ttgcatgttg cgtaaagacg atccgcagtt caaaaagctg atggatgaca ctatcgctca
    30361 ggtgcagacc tccggtgaag cggaaaaatg gtttgataaa tggttcaaaa atccaattcc
    30421 gccgaaaaac ctgaacatga atttcgaact gtcagacgaa atgaaagcac tgttcaaaga
    30481 accgaatgac aaggcactga actaattaca agaaccagag gcggaaattc cagccctctc
    30541 gattgttacg tagcacggac agactatacg cctgatggtc gttccccatc gggcctgaaa
    30601 accgcaatac gctgggtaac aatcttcgag ggtagcagtt aacgctgcta ccctcttttt
    30661 tctggagtag atttatgtct atagactgga actggggtat ttttttacaa caagccccgt
    30721 tcggcaatac cacctatctt ggttgggtct ggagtggttt tcaggtgacg atcgctttgt
    30781 cgatctgcgc ctgaattatc gctttcctcg tcggttcatt tttcggcatt ttacgtaccg
    30841 ttccaaaccg ttttctctcc ggtctgggta cgttgtatgt cgaactgttc cgcaacgtgc
    30901 cgttgattgt gcaattcttt acctggtatc tggtgatccc ggagctgctg ccggagaaaa
    30961 tcggcatgtg gtttaaggcc gagctggatc ccaatattca gtttttcctt tcatccatgc
    31021 tctgcctggg gctgtttacc gcctcccgtg tttgcgaaca ggtacgcgcc gctattcagt
    31081 cgctaccgcg tgggcagaag aatgccgcgc tggcgatggg cctgacgcta ccgcagactt
    31141 atcgatacgt actgctgcct aatgcttatc gcgttatcgt cccgccgatg acttccgaga
    31201 tgatgaacct ggtgaaaaac tccgccatcg cctcgaccat cggtctggtg gatatggcgg
    31261 cgcaggcggg taaattgctc gactactcgg ctcacgcctg ggagtcgttt accgccatca
    31321 cgcttacgta tgtgttgatt aacgctttta tcatgctggt gatgacgctg gttgaacgta
    31381 aagttcgcct gcccggcaac atggggggca aataatgtac gagtttgact ggagttccat
    31441 tgttccttcc ctgccatatc tgctcgacgg gctggtgatc accctgaaaa tcaccgtcac
    31501 ggcagtcgtg attggtattt tgtggggtac catgctggcg gtgatgcgct tgtccagctt
    31561 cgcgcctgtt tcgtggtttg ccaaagccta cgttaacgta ttccgctcga ttcctttagt
    31621 catggttttg ctgtggtttt acttgattgt gccgggattt atgcaaaatg tgctgggatt
    31681 atcgccaaaa aatgatattc gtttaatttc ggcgatggtg gcgttttcga tgtttgaagc
    31741 ggcctactat tcagagatta tccgtgccgg tattcaaagt atttcccgtg gtcagtcgag
    31801 cgccgcgctg gcgttgggga tgactcactg gcagtcgatg aaactgatta ttctgccgca
    31861 ggctttccgc gcgatggtgc cgctgctgct cactcagggc atcgtactgt tccaggatac
    31921 ctcgctggtg tatgtgttat ctctggccga tttcttccgt accgcctcaa ccattggtga
    31981 gcgtgatggt acgcaggtcg agatgatcct gtttgccgga tttgtttatt tcgttattag
    32041 ccttagcgcg tcgttgttgg tcagctactt gaaaagaagg acagcataat gattaccctg
    32101 aaaaatgttt caaaatggta tggtcacttt caggtgctga ccgactgctc aaccgaagtg
    32161 aaaaaaggcg aagtggtggt ggtttgcggc ccgtctggtt ccggcaaatc aacgctgatt
    32221 aaaaccgtca acggcctcga accagtgcag caaggtgaaa tcaccgtcga tggtatcgtg
    32281 gttaacgaca agaaaaccga tctggcaaag ctgcgctccc gcgtcgggat ggtattccag
    32341 catttcgagc tgttccctca tctgtcgatt atcgaaaacc tgaccctggc gcaggtgaaa
    32401 gtgcttaaac gcgataaagc gcccgctcgt gaaaaagcat tgaaattgct ggagcgcgtg
    32461 ggactttccg cacatgccaa taaatttccg gcgcagcttt ccggcggtca gcagcagcgt
    32521 gtggcaatcg ctcgcgcatt gtgtatggct cctattgcga tgctgttcga cgaaccgaca
    32581 tcggcgctgg atccggagat gatcaacgaa gtgctggacg tgatggtgga actggcgaac
    32641 gaaggaatga ccatgatggt ggtgacccac gaaatgggct ttgcccgcaa agtggcgaat
    32701 cgggtgatct ttatggacga gggtaaaatt gtcgaagact cgccgaaaga cgctttcttc
    32761 gatgatccga aatcggaccg cgcaaaagac ttcctcgcga aaatcctgca ttaatcactc
    32821 tggcgcgcat acccaattgc gcgccatctc acgctttatc gtttcgcccc tcgtatctgt
    32881 tacatcctgg cgttaccctt gtcgcaaaga agcacacaac aaggagcaac aatggcactg
    32941 ccaattctgt tagattgcga cccaggtcat gatgacgcta tcgcaatagt tctcgccctc
    33001 gcctcaccag agcttgatgt caaagcaatt acgtcttccg ccggaaacca gacaccagaa
    33061 aaaaccttac gcaatgttct gcgtatgctg accttgctta atcgcaccga tattccggtg
    33121 gcaggcggcg cggtaaaacc gttaatgcgt gagttgatta tcgcggacaa tgtgcacggc
    33181 gaaagcggtc tcgacggccc ggcattaccg gaaccgacgt tcgcaccgca aaactgtacg
    33241 gcggtggagc tgatggcgaa aacgctgcgc gaaagtgcgg aacctgtcac gattgtttct
    33301 accggaccgc aaactaacgt tgccttgctg ctcaatagcc atccggaact gcatagcaaa
    33361 attgcccgta tcgtgattat gggcggcgca atggggcttg gtaactggac gcctgcggct
    33421 gaatttaata tttacgttga cccggaagcg gcagaaattg tcttccagtc agggatcccg
    33481 gtggtgatgg ccggtctgga tgttactcat aaagcgcaaa tccacgttga agacaccgag
    33541 cgtttccgcg cgattggcaa ccctgtttca accattgttg ccgaactgct ggatttcttc
    33601 ctcgaatatc ataaagacga aaaatggggc tttgtcggcg caccgctgca tgacccatgc
    33661 accatcgcct ggctgttgaa acccgagttg tttaccactg ttgagcgctg ggttggcgtg
    33721 gaaacacagg ggaaatatac cctgggtatg acggttgttg attattatta tctgactggc
    33781 aataaaccga atgccactgt aattgtcgat gttgatcgtc agggctttgt tgatttactg
    33841 gctgatcgtc tgaaatttta cgcttaacac tcgtcattga ttaagatcac cacgcgttac
    33901 ctgggtggtc ttaatctatt tatctctttt tctttcaaga tataaatgca atggataatg
    33961 cagaactcgc cataggtatc gatctcggca ctaccaatag tttaattgcc gtctggaaag
    34021 acggtgccgc gcaattaatt ccaaataagt tcggtgaata tttaacacca tccataatta
    34081 gcatggatga aaataatcat attttagtcg gaaaaccggc tgtatcacgg cgtacttcgc
    34141 atccggataa aacggcagcg ttatttaaac gtgcaatggg cagtaatacc aactggcggt
    34201 taggcagcga cacatttaac gcgccagaac tgtcctcttt ggtattacgc tcattaaaag
    34261 aagatgccga agaatttctg caacgtccga ttaaagatgt ggtgatctcc gttccggctt
    34321 atttcagcga taaacaacgc aagcataccc gtttagcagc ggacttagcc gggttaaatg
    34381 cggtacgctt aattaatgaa cccacagcag ctgcgatggc gtatggcctg catacccaac
    34441 aaaatacccg ttcgctggtg tttgatctcg gtggcggcac gtttgacgtt acggtgcttg
    34501 agtacgccac gccggtgatt gaagttcacg cctccgctgg cgacaacttc ctcggtggcg
    34561 aagattttac ccatatgttg gtcgatgacg ttttaaagcg cgcggatgtc acaaggacca
    34621 cgcttaacga gagtgaactg gcagccttgt acgcctgtgt ggaagcggca aaatgtagca
    34681 atcaatcgcc aatgcacatt cgctggcagt accaggatga aacgcgggaa tgcgaatttt
    34741 acgagaacga actggaagat ttgtggttgc cgctgctcaa tcgcttgcga gtgccgattg
    34801 aacaggcgtt gcgcgatgcg cgtctgaagc cgagtcaaat cgacagtctg gtgctggttg
    34861 gcggcgcgtc acaaatgccg ctggtgcagc gaatcgccgt gcgtctgttt ggcaaattac
    34921 cgtatcaaag ttacgatccg agcaccattg tcgcgctggg cgcagcaatc caggccgcct
    34981 gccgcttacg cagtgaagat attgaagaag tgatcctcac tgatatttgc ccttactcgt
    35041 tgggcgttga ggttaaccgc cagggcgttt ccggcatttt ctcgccgatt attgaacgaa
    35101 acaccactgt gcccgtgtcg cgtgtagaaa cttattcaac catgcacccg gaacaggatt
    35161 caattacggt taacgtctat cagggagaaa accacaaagt taaaaacaac attctggtgg
    35221 aatccttcga tgtgccgttg aagaaaaccg gggcttatca gtcgattgat attcgcttta
    35281 gttatgatat caacgggttg cttgaagttg acgtgcttct ggaagacggc agcgttaagt
    35341 ccagagtgat taaccacagc ccggtaacat tgagcgcgca gcagattgaa gagagtcgga
    35401 cgcggttatc cgcattgaaa atttatccgc gcgatatgct catcaatcgc acctttaaag
    35461 ccaaactgga agagttgtgg gcgcgggcgc tgggtgacga gcgagaagag attggccggg
    35521 tgatcaccga ttttgatgcg gcgctgcagt caaacgatat ggcccgcgtc gatgaggtca
    35581 ggcggcgggc gagcgtttat ttagccattg agacctcata atcacggctg ccggatgcgg
    35641 cgtaaatgct gcatccggca tcggcgcaaa aaacacatcg tcaactaaac aacatcgcga
    35701 ttttgccgag agcataacct ataaccagca ccgttaaact gattttgata atgctggtaa
    35761 tgctctgtgg tttttcaccc tcatcatttt tttcttcatg gttttcaggt gttggctcat
    35821 cgacatcttc atcctccggc tcgagtttta ctttcgtcaa cagcatccgg gtgataatgg
    35881 cctctaatgg atgagcatct ccgccttcca gccttgtatt aagacgggcc agcatctctt
    35941 gtttcggaaa cgcttcaagt actacctgtt ccgctacccg atacaaatcc agtaacggtg
    36001 aatcttcttc tgtctcgcta ccgactctcg atttccacca cgcccatgct ttgcgtttga
    36061 agaataacga ctcaataaac gattgaccta acggcgataa ttttccgcta tgtaaaattt
    36121 ccgcccataa cagccgcgtc cgggcgctct caccaggaac atatttcagc ggttgacctt
    36181 gcgcatctac catgctccgc gcttcgataa ccatcactaa taacgcgaaa taatccgggc
    36241 aatgacgctt gctccactgc aacaacaaat tatcagcttg tgtagatggg taactttccc
    36301 agtacgcgcc gagatcagca agcaggcttt ccccctcgcc gcagtacaaa cgttgtgcac
    36361 actggtaata ccgggcatct tcgctttctg gttcttccgc ttgccactgt ttggctacag
    36421 gaatgagttc caccatacct gtttgcaccg agctgtacca gcgcaacagt ttgcgatgta
    36481 actttgcatc atcaggaatg aaccacggtc cttccatcgc caaaaacgca gtgacatagt
    36541 caggatggta acggcagcaa cgttccagcg cgaagtaata ttcaacagtc tggttctgta
    36601 acgcgacggg atattccaga aggctaagga aatcgaaaca atcaccagcc tgaatggcct
    36661 caagaaaagc ctccctttct tcctcatcca ttccttcgct gtcattaaac gaatgccagt
    36721 tcaaacgctc tgccaacaaa gaagcacaat ttaatgacag atatcgcgct tctatagcga
    36781 ttgcacacag cggccagcgt aattgatcga catcctccat gttccaggta tttaattgct
    36841 gaataaattt ctgccaggcg gagggctgaa aacgatcact ttctgaatcc agtaatgtcc
    36901 tgaatgcacg tagtatttca tgttcagcgg tggcatcttt ttcttcatca tctgcttcct
    36961 cgacaggatt taccgccagt cgcagcgcct cttcataggc ctggcgtaat gctttaaacc
    37021 cctgcggatc ggtttcggga tgacacaacg gtaagcgagc aagataagcc tggcggataa
    37081 tgtctatttg cgtcgtgctt tcaatttcga ggatttgcca acatgttttc ataggtcgct
    37141 cacatcctta agcgttaccg ggcattcagg ctggacagtt aatggctgcc agggaagcgt
    37201 ggcatagaaa cggtcgcttc ccgctgccag ctcttcgaac atccgactcc cggcgctatc
    37261 gtatttgtac agggttagcg cctcgcgtag cgaagcgttg tcttcgcact gagaagattg
    37321 ccagtagaga cgccctagtg aataggcaga aaaatagtgc atccagctgt gatagtaatg
    37381 gtgagctcgt gcatagacgc gcgattgcag ccataaactc tcttcttccg tcagccagtt
    37441 gttgagcaca ccggcccgtg acagaaaccc cattcgaaca taatcccagg ctttgattcc
    37501 cccttctcca cagcacatag cggtttgggc aacgaattgg gtataaatca accctctctc
    37561 cgagccacca gcaattaatg ctttccactc ttcaggggaa gagcgttgcc atgtcagata
    37621 taagccagct aaatcattgg catgaccatc atcagtcatc tgataaatag tctgaattaa
    37681 atcatcacga ttatttattc cccaggatga tgaga
//
LOCUS       DAJVQH010000020        37646 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJVQH010000020 DAJVQH010000000
VERSION     DAJVQH010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.020"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..174)
                     /locus_tag="OQM87_001182"
     CDS             complement(<1..174)
                     /locus_tag="OQM87_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCS1507393.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="OQM87_001183"
     CDS             complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="OQM87_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1507394.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1228..1524)
                     /gene="fis"
                     /locus_tag="OQM87_001184"
     CDS             complement(1228..1524)
                     /gene="fis"
                     /locus_tag="OQM87_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCS1507395.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="OQM87_001185"
     CDS             complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="OQM87_001185"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCS1507396.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="OQM87_001186"
     CDS             complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="OQM87_001186"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCS1507397.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3737..5188)
                     /gene="panF"
                     /locus_tag="OQM87_001187"
     CDS             complement(3737..5188)
                     /gene="panF"
                     /locus_tag="OQM87_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCS1507398.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5178..5420)
                     /locus_tag="OQM87_001188"
     CDS             complement(5178..5420)
                     /locus_tag="OQM87_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCS1507399.1"
                     /translation="MDTSFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5529..6878)
                     /gene="accC"
                     /locus_tag="OQM87_001189"
     CDS             complement(5529..6878)
                     /gene="accC"
                     /locus_tag="OQM87_001189"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCS1507400.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHIEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6889..7359)
                     /gene="accB"
                     /locus_tag="OQM87_001190"
                     /gene_synonym="fabE"
     CDS             complement(6889..7359)
                     /gene="accB"
                     /locus_tag="OQM87_001190"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCS1507401.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8337..9311)
                     /locus_tag="OQM87_001191"
     CDS             complement(8337..9311)
                     /locus_tag="OQM87_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1507402.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9463..11403
                     /gene="csrD"
                     /locus_tag="OQM87_001192"
     CDS             9463..11403
                     /gene="csrD"
                     /locus_tag="OQM87_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCS1507403.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11708..12751
                     /gene="mreB"
                     /locus_tag="OQM87_001193"
     CDS             11708..12751
                     /gene="mreB"
                     /locus_tag="OQM87_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCS1507404.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12817..13920
                     /gene="mreC"
                     /locus_tag="OQM87_001194"
     CDS             12817..13920
                     /gene="mreC"
                     /locus_tag="OQM87_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCS1507405.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13920..14408
                     /gene="mreD"
                     /locus_tag="OQM87_001195"
     CDS             13920..14408
                     /gene="mreD"
                     /locus_tag="OQM87_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCS1507406.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14417..15010
                     /gene="yhdE"
                     /locus_tag="OQM87_001196"
     CDS             14417..15010
                     /gene="yhdE"
                     /locus_tag="OQM87_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCS1507407.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15000..16469
                     /gene="rng"
                     /locus_tag="OQM87_001197"
     CDS             15000..16469
                     /gene="rng"
                     /locus_tag="OQM87_001197"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCS1507408.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16537..20337
                     /gene="yhdP"
                     /locus_tag="OQM87_001198"
     CDS             16537..20337
                     /gene="yhdP"
                     /locus_tag="OQM87_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCS1507409.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVDGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20493..21938
                     /gene="tldD"
                     /locus_tag="OQM87_001199"
     CDS             20493..21938
                     /gene="tldD"
                     /locus_tag="OQM87_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCS1507410.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFSYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="OQM87_001200"
     CDS             complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="OQM87_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCS1507411.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23178..23381
                     /locus_tag="OQM87_001201"
     CDS             23178..23381
                     /locus_tag="OQM87_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCS1507412.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23389..24321
                     /gene="aaeA"
                     /locus_tag="OQM87_001202"
     CDS             23389..24321
                     /gene="aaeA"
                     /locus_tag="OQM87_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCS1507413.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24327..26294
                     /gene="aaeB"
                     /locus_tag="OQM87_001203"
     CDS             24327..26294
                     /gene="aaeB"
                     /locus_tag="OQM87_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCS1507414.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26386..26658
                     /locus_tag="OQM87_001204"
     CDS             26386..26658
                     /locus_tag="OQM87_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCS1507415.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTLGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26714..26977)
                     /gene="yhcN"
                     /locus_tag="OQM87_001205"
     CDS             complement(26714..26977)
                     /gene="yhcN"
                     /locus_tag="OQM87_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCS1507416.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27342..27812)
                     /gene="argR"
                     /locus_tag="OQM87_001206"
     CDS             complement(27342..27812)
                     /gene="argR"
                     /locus_tag="OQM87_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCS1507417.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28247..29185
                     /gene="mdh"
                     /locus_tag="OQM87_001207"
     CDS             28247..29185
                     /gene="mdh"
                     /locus_tag="OQM87_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1507418.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29247..30314)
                     /gene="degS"
                     /locus_tag="OQM87_001208"
     CDS             complement(29247..30314)
                     /gene="degS"
                     /locus_tag="OQM87_001208"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCS1507419.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30404..31771)
                     /gene="degQ"
                     /locus_tag="OQM87_001209"
     CDS             complement(30404..31771)
                     /gene="degQ"
                     /locus_tag="OQM87_001209"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCS1507420.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDEKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTNTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31925..32323)
                     /locus_tag="OQM87_001210"
     CDS             complement(31925..32323)
                     /locus_tag="OQM87_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCS1507421.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32517..33644
                     /gene="zapE"
                     /locus_tag="OQM87_001211"
     CDS             32517..33644
                     /gene="zapE"
                     /locus_tag="OQM87_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCS1507422.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33863..34291
                     /gene="rplM"
                     /locus_tag="OQM87_001212"
     CDS             33863..34291
                     /gene="rplM"
                     /locus_tag="OQM87_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCS1507423.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34307..34699
                     /gene="rpsI"
                     /locus_tag="OQM87_001213"
     CDS             34307..34699
                     /gene="rpsI"
                     /locus_tag="OQM87_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCS1507424.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35094..35732
                     /gene="sspA"
                     /locus_tag="OQM87_001214"
     CDS             35094..35732
                     /gene="sspA"
                     /locus_tag="OQM87_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCS1507425.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35738..36235
                     /gene="sspB"
                     /locus_tag="OQM87_001215"
     CDS             35738..36235
                     /gene="sspB"
                     /locus_tag="OQM87_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCS1507426.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36278..37645)
                     /gene="dcuC"
                     /locus_tag="OQM87_001216"
     CDS             complement(36278..37645)
                     /gene="dcuC"
                     /locus_tag="OQM87_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1507427.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9115 a         9414 c         9904 g         9213 t
ORIGIN      
        1 tctgtcctga aaattcagtt cgtcacagtt aaccagtttt agtggattaa ctgagttcat
       61 attacggcat ttgtctgttt ctgttgtcag cagttcaatc cactgcatca gtaaagtatc
      121 aaacagaaaa acgaagacag cgaaaacgac gagccaccaa tacttgcgaa tcattacctg
      181 agcctgtgca tgtttaacca agtctggcag gaaatataca cgaaatgaat aatctgaaaa
      241 ccaggcttac acttccttta ctttgcaatg agatcggggt caacttcgct taaccggctt
      301 cgttttgaca ggttgccggt ctttcttttt ttcactttcg ccagttcttc cgcggagtaa
      361 tgcgacgcga catccagccg tcgaagcccc attttgatgt actcgctgtt gatctcaata
      421 ccaatgaatt ttcgtccgct ggcgatggct acggcaccgg tagtaaagct accggcaaac
      481 gggtcgagaa cgatatcgcc tgggttggaa gaggcgagaa taatgcgttt cagtaaggct
      541 ttcggttttt gcgtcgggtg gttctcatat tcatccatca aataacgcac gcgtggaaaa
      601 tcccagacgt tacccggtac tttttgatga ttgtatggct gtggaggatt tttgcgataa
      661 tagatcaacg cgcgctgcga tccggttttg gcttctacca gaatagcatc accgttgaat
      721 gtgtagttct ttgcgtcttt caccatcatc aggatgggtt cgtacatgga gccgtagtgt
      781 tttttcgcct gtactccaga actgtcatat gaccagacga tgcgactttt gatggtaaaa
      841 agcttgcggc actggagatc gataaagggc atgttttccg tactgttcat aatgtacatg
      901 ctgccctgct ttttcagaac gcggtggcac tctgcaatca cttcaaacag ccagtcgata
      961 aacagatctt ctttccaggc ttcgatcaaa ccatcaaaat ttttaccgat gttatacggt
     1021 gggtcggcaa agagcagatc gacgctttca gtaggtagct ttttaagttc ggcaagggca
     1081 tcaccgtgaa taatggtctt agcttcatta ccaaaccggg tcggttcaca tcctgttctc
     1141 atggtcactc cctttgtgac acctataaaa aaggcgcttc cctatgccga gtagcgcctt
     1201 tttaatcaag catttagcta acctgaatta gttcatgccg tattttttca attttttacg
     1261 cagcgtacca cggttgatgc ccatcatcag cgcagcacgg gtctggttac cacgggtgta
     1321 ttgcatcacc atgtccaaca ggggctgttc tacttcagcc agtaccagct catagaggtc
     1381 attcacatcc tgaccattca gttgagcaaa atagttcttc agtgcctgtt taaccgagtc
     1441 acgcaggggt ttttgggtta cctgatcctg agagttaacg gtagaaacgg tcagtacgtc
     1501 agaatttacg cgttgttcga acatagttct gtcagctctt tatttctgtt tacgcaaaat
     1561 tttcgaagta tgcctccaac gcctccagct gttcgctggc atcctcaatg gcgttgaatg
     1621 tgcgccgaaa ctggtcattt ggagcgtgtt cctggagata ccaggaaacg tgtttacgtg
     1681 caattcggta cccttttgcc ggaccataaa agtcatgcag ttcccgaacg tgcgcgcaaa
     1741 gcaagcgctt aacctctgcc aaaggcagcg ggggcagcaa ctccccagtg tccagataat
     1801 gctggatttc ccgaaagatc cagggtcttc cctgagctgc gcggcctatc atcagggcat
     1861 ccgcccctgt atagtcgagc acagctctgg ctttaagcgg gtcagtaatg tcgccattcg
     1921 cgataaccgg aatggaaact ttctgcttaa ctgcccgaat actgtcgtac tcagcttctc
     1981 cattgaacaa acaggcgcgt gtacggccat gaatggtcag agcctgaatg ccacagtctt
     2041 cagccagttg ggcaatctct tcgcagttac ggtgttccgg tgcccagccg gtgcgaatct
     2101 tcagggtaac aggaacgtcc actgcattga cgacctcggt aaggatcgat ttaacgacat
     2161 ccgggtactg caagagggct gaacctgcga gcttgcgatt cactttttta gccgggcaac
     2221 ccatattgat atcaataatc tgggcaccgc tttccacgtt aatacgtgct gcatctgcca
     2281 tttctttcgg agcgctacca gcaatttgca cggtgcgaat accgggttca tcaatgtgca
     2341 ccatccgtaa acgagatttg tcgctttccc aaacctgtgg gttagaagac atcatctcgg
     2401 atactgtcaa tccggctccc atctcgtagc acaacgtccg aaaaggtctg tctgtaatgc
     2461 cagccatggg cgctgcgatc aggcgatttc tgagctgata ttgtccgatg cgcatgagtt
     2521 aagaaatgac catactgtga ctgcaaggcg gcgtatatta cgcatttttt gcacgagatg
     2581 aaaggccaaa ctttgaccaa tcctctgaga tggatcaaag aattgcattt aaaatgagcg
     2641 tggtgcgata attactcata aaaatcatca tattagaaaa tagtgactaa aatttacact
     2701 caaagaaatt tgagtaagtt ctcaattttt ctttatgaat gaaaattttg gcacgcaaat
     2761 ttgcgtaaat aatcggcaaa tttacgtgcc ttttgtgagc ttgctcgcac ttcgccccgc
     2821 gtcaccctac ggcgatgcga aggttaattc ttacgaccgg taatacggca ccactcttct
     2881 ttttccacga ccgggtccag tgcgaagcta tcggcataag cttcacaaac gctctctgcc
     2941 tggcttgcca gaataccgga aaggcccagc aaaccgcctg aaaccggcag gacgctgatt
     3001 aacggtgcca gttcacgtaa tgggcctgca aggatgttag cgaccaccac gtcggctttc
     3061 atttcttctg gctgatcttt cggtaagtag agttccagac ggtcagaaac gccattacgt
     3121 tcggcgttat cgcggctggc ctgaatcgcc tgcggatcga tatcaatacc aatggctttt
     3181 gctgcaccca gtttcagcgc cgcgatcgcc agaatgccgg aaccacagcc aaagtcgatg
     3241 actgttttac cggttaaatc gaggctgtcg agccattgca ggcacagaga ggtggttgga
     3301 tgggtacccg taccaaacgc cagccctgga tctaacatca cgttgacggc gttttcgtcc
     3361 ggcacatcac gccagctagg gcagatccac agtcgttcac caaagcgcat cgggtggaaa
     3421 ttatccatcc attcgcgctc ccagtcttta tcttctagtt gttcggtttt atgcgcgaag
     3481 cctgcgccga gcagcggatg gttttccaga atcgccacca cgtcgttcat atcggtttca
     3541 gcgtcgaaca gaccaatcac atcggtgtcg ccccacaggc gcgtttcgcc cggcagcggc
     3601 tcaaatactg gcgtatcgtg ggtatcctga aaagtgatag aaacggcacc cgcttccatc
     3661 agcgcatcgc taagatcttc cgcgttcgcg ccggtggtgt tcagtttcag ttggatccaa
     3721 ggcatggcaa aactctttat ttatcagtag tcaaaacggt agcttgcggg acggatgtac
     3781 cgaaacggtt tccgaccagg aaagccagca aacttagtag taacgagggc acgatagggt
     3841 ggaagcccag gtactgaata ttcagcgtcg cgagtacggc atacagcacg ccgccaacga
     3901 tcatcgcact tagcgcgcct ttggcgttgg cgcgttccca gtaaagaccc agcaccagcg
     3961 gccacaggaa aacggcttcc agcccaccga aggccagcaa attcagccag atgatcattt
     4021 ctggcggctt ccaggcggca agcagcagca acgcgccgag aactaacgta attaccgccg
     4081 acatccgctt cagacgcgtc tcgttttgca tttgatccgg acggatattc agatagagat
     4141 ctttaatgat cgtagcggaa ctttgcagca gttgggcgtt aattgtcgac atgatcgcag
     4201 ccataggtgc agccagaaag atcccggcag caaaaggtgg cagcactttt accattaacg
     4261 tcgggatcac cagatccggt acggtgagat cggggatcac tgcacgacct aacgctccgg
     4321 ctaggtgcat accgaacatc aggattgcga ctacaatcgt accgatgatg atcccccggt
     4381 gtactgcttt gctgtcttta taagagatac agcgcaccgc agtatgcggc aggccaatca
     4441 cgccaaagca caccagtacc cagaacgacg tcataaaggc aggcgacaga atatcgtcag
     4501 cgccttgtgg cgtaaccagt tgcggatcga tggtttgcaa ggtctgtact gcgttgctta
     4561 agccgccagc ggcatgtacc acgccaataa gcagcacaac ggtgccaatc agcatcacaa
     4621 gcccttgcat ggtgtcgttc agcacgctgg cgcgaaagcc accaaaggcg gtatataacg
     4681 caatgctgat accaaaaatc agcagcccgg tttcataagg aatacccgcc gcggtttcca
     4741 gcaggcgcgc accgccgata aactgcacgg tcatcgcacc aacgaacgca accagcaaac
     4801 tcaaactcgc cagccacacc agaagacgac tctggtagcg ggcaaacagc atatcgttca
     4861 gcgtcactgc attgtagcgg cgcgcaagaa tcgcaaactt cttgccgaga ataccgagtg
     4921 aaagccagac agcaggaagc tgaatcatcg ccagcaatac ccagcccagc ccgtatttat
     4981 aagcagctcc tggcccgccg ataaacgaac tggcactgat ataggtcgcg gtgagcgtca
     5041 tcgccagcac aataccgccc atagagcggc tgccgaggaa atactcatta aggaaggtgc
     5101 cggtgctccg tttacgcatc gcataaaccg agataccgaa caccaccacc agataggcga
     5161 ccagcggtag aattacttca agctgcatcg tcatcctcca gtgggatatc gcgatagata
     5221 aatttcacca tcgcccagca cagtccaata aacagtagcg gcgtcaggat gcaggccatc
     5281 tcaaaccagc gcggaaagcc ggtaaaaccg ggggcaacgc caggtaagta agcatctact
     5341 aaccaaactg ccagatacaa aagggtcagc cccagcgccc agcgcgcctc tttatgggcc
     5401 tgaacaaaac tagtgtccat tttttgtccc tgatgggtga agaaagcgag gattgtacct
     5461 tatgggggtt gtcgatcccc agtaataaaa aaggccggaa aatccggcct tttgacgctt
     5521 ttgcagtctt atttttcctg aagaccgagt tttttctcca gatagtggat gttagtgcca
     5581 ccatgctgga agttctcgtc attcatgatg cggatctgca gatcaacgtt ggttttgata
     5641 ccgtcgatga tcagctcctg cagcgcattc ttcatgcggg caatcgccac gtcacggttt
     5701 tcaccgtagc aaatcagctt accgatcatt gagtcatagt acggcggtac ggtgtagccc
     5761 gcgtagatat gagactccca acgtacgcca aaaccgccag gtgcgtggaa acgggtgatt
     5821 ttgcctgggc ttggcaggaa ggtgttcgga tcttcggcgt tgatacgaca ttccaccgca
     5881 tggccgcgaa cgtgaacttc ttcttgcttg atcgacagcg gttgaccggc agcgatacgc
     5941 agctgttctt tgatcaggtc aacgccggtg atcatttctg taaccgggtg ttctacctga
     6001 atacgggtgt tcatttcgat gaaatagaac tcgccgtttt cgaacaggaa ctcgaaagta
     6061 cctgcaccgc gatagccgat atcaacacac gctttagcgc aacgttcgcc gatgtagcga
     6121 cgcagttccg gggtaatgcc cggtgctggt gcttcttcga ccactttctg gtggcggcgc
     6181 tgcatggagc agtcacgttc cgccagatag atagcgttgc cctgaccgtc agccagtacc
     6241 tgaatctcga tgtggcgagg attctccagg tatttctcca tgtaaaccat atcgttgctg
     6301 aaagcagctt tcgcttccgc acgggtcatg gagatggatt gtgccagttc agcgtcgccg
     6361 cgcactacgc gcataccgcg accgccgccg ccgccggagg ctttgataat caccggataa
     6421 ccaatgcgtt tagcaatggc gcggttttta tccatatcgt cgcccagcgg gccgtcagaa
     6481 ccaggtacgc aaggaacgcc cgcttttttc atcgccgcga ttgcggatac tttgtcgccc
     6541 atcaggcgaa tggtttctgc tttcgggccg atgaagataa agccggagcg ttcaacctgc
     6601 tcggcaaagt tggcgttctc ggagaggaag ccgtaacccg gatggattgc tactgcgccg
     6661 gtgatttcag cggcgctgat gattgccggg atgttcagat aactttgtac tgacggagca
     6721 gggccaatac agaccgtttc atctgccagt aatacgtgtt ttagatcgcg atccgcgctg
     6781 gagtgcacag cgacagtctt gatgcccagt tctttacagg cacgaagaat acgcaatgca
     6841 atctcgccgc ggttggcaat aacaatttta tccagcatgt tcgcctcgtt actcgatgac
     6901 gaccagcggc tcgtcaaatt ctaccggttg tccactttcg accagaattg ctttcacggt
     6961 accggatttg tccgcttcga tctggttcat cattttcatg gcttcaacga tgcacagggt
     7021 atcgcccacg ttgactttct gacccacttc gatgaacgct tttgcgtccg ggcttggggt
     7081 gcggtagaaa gtaccaacca tcggggaacg tacgatgtga ccactgattt ccgctgctgc
     7141 tggcgcttcc atggaaggaa cggtcgccgg agcggctgcg ttagattgag ctggctgctg
     7201 catcattggt gcagcgtaag cttgttgcat cacagggaaa cttgcggcag gagctgcacg
     7261 gctaatgcgt actgactctt cgccttcaga aatttccagt tcggagatgc ctgattcttc
     7321 aaccagctcg atcagttttt taatcttacg aatatccatg agtgggttcc gtactctttg
     7381 tttagtgtga ttgtgacagg cgtttcaccg ccgtctgtaa agcgtatgcc ccgacagctg
     7441 tatgcatagc gataaattcc agcaggccgg gacgccggtc tattttgccc cagagtgtca
     7501 atgttagact tgacggacat tgtgcagcga tctgctgcta tcctccggca aaaaacaaaa
     7561 tataccttcg ttgcgccaat gtcacctttt ccgcagcgca aaaactgcgt cagggaggac
     7621 gaggtcgcac attataacga tttcgtagca attggcagct aaatactggt cttatcaggg
     7681 aagataatca acagctaaca tgtaaataac cttcaacacc gtgtaatttg caacaagccg
     7741 cacaattcac gaaattagcg ccatcatcga cggaacttct tataacgtaa ggtgaaaagc
     7801 tgaaaagcca gccctgcgaa gatgaggggc tgcaatgaga taatcttcac agaccacaaa
     7861 taatgtaggg atgccaggat cgcgacaaga tagacgacat tacgcagcgg ttgctgggga
     7921 ttacccaatt ttcactgcat cgtctggata gatgtgaatg ctaaagcacg caaaatcaac
     7981 caactgacaa ttctcagcat taaataggtt cgagtggtta atttgtagtc aaataaaatc
     8041 agattattca ccccatggta ccagaaatca gtaacaggtt aagcgtagtg tcgcccaggc
     8101 aaagcaccat aaacctaaca ggcgacggag gcgtatcaat aatggctgtt tagaggagta
     8161 tcaatgcgca tcaatttgtg gtcagtcgaa atcatgactt tccagtttgg atgatgttgt
     8221 acaacttcct gaccaaggtg ttcgacgaaa gctgtccggc taaccgcttt tcccgttaac
     8281 cgattgaata atgatgtcaa aatgagttcc ctctcttatt attccttgct aaatggttag
     8341 ttaaccttca ccagcgtgcg accttggatc tggttattaa tgatggcctc ggcaaagttc
     8401 ggtgcctctg acagagatat ctcttttgcc gcctgggtat agaatgattc cggtaaatcg
     8461 gcgaccagtc gctgccaggc ttgtgcgcgg cgttctggtg gcgtcattac tgaatccacc
     8521 ccttgcaaac ggacattacg cagaataaat ggcatgaccg tgcttggcag agtaaaacca
     8581 cccgccagac cacaggccgc cacgcagccg ccgtaattca tttgcgccag cacttttgcc
     8641 aggactttgt cgccaacagt gtcaattgcc ccagcccaga cttgtttttc cagaggacgg
     8701 gattcggcaa actcatcacg agggagaata cggctggcac ctaaactttt cagatattca
     8761 tgggtacttt cgcgaccgga aacggcaacg acctgataac ccaacttatg cagcagcgcc
     8821 acggcggtac tgccgacgcc accactggca cccgtcacga caatctcccc gtcctgcggg
     8881 cgaacaccgg catcttccag cgccatcaca cacagcatgg cggtaaaacc ggcagtaccg
     8941 ataatcattg ctttacgcgc gtccagccct tgcggcatgg caaccagcca gtcacctttc
     9001 actcgcgcct gctccgccag cccaccccag tggttttcac caacgcccca gccagtgagt
     9061 aacacctcct gaccggcatg aaaacgcgga tcttcgctgg tgcgtacagt tccggcaaaa
     9121 tcgatcccag gaatcatcgg aaaattacgg atgatttttc ccttaccggt aatcgccagc
     9181 gcatctttat agttcaggct cgaccagtga acatcgaccg tgacatcgcc ctccggcagg
     9241 cgactttcgt ccagagtctg tactgatgcg agagttttgc cgtcctgctg ttctaaaagt
     9301 aacgcctgca taagtggtcc tcatgtgcat gatggattgg aaaataattt ctgaagacta
     9361 tactcgctaa tggaaaagca aagccgatga agcgcaataa attgcgagat aaatctgatt
     9421 tgctagtatg cccgcttcct cactatcgga gttaacacaa ggatgagatt aacgacgaaa
     9481 ttttcggcct ttgttacgct gctcaccggg ttaacaattt ttgtgacttt gctgggctgt
     9541 tcgctaagtt tctacaacgc cattcagtat aagtttagtc atcgcgttca ggcggtggcg
     9601 acggcgattg atacccacct tgtgtcgaat gacttcagcg cattaaggcc acaaattacc
     9661 gaattaatgg tgtcagcaga tatcgttcgt gtagacctgc tgcatggtga taaacaggtt
     9721 tataccctgg ccagaaatgg tagttatcgt ccggttggca ccagcgatct gtttcgtgaa
     9781 ctgagcgttc cgttgataaa gcatccgggg atgtcgttgc gtctggttta tcaggatccg
     9841 atgggcaact atttccattc gttgatgacc accgcgccgc tcacgggggc gattggcttt
     9901 atcattctta tgctctttct ggcggtacgc tggttacaac ggcaacttgc cgggcaagaa
     9961 ttgctggaaa cccgggctac tcgtatctta aacggtgagc gtggctctaa tgtgttggga
    10021 accatctatg aatggccgcc cagaaccagc agtgcgctgg atacgctgct tcgtgaaatt
    10081 cagaacgcac gcgaacaaca cagccgtctt gatacgctga tccgctctta tgccgcacag
    10141 gacgtgaaaa ccggcctcaa taaccgactc tttttcgata atcagttagc aacgttactg
    10201 gaagatcagg agaaagtagg tacccacggg atcgtgatga tgattcgtct gccggatttc
    10261 aatatgttga gcgatacctg ggggcacagc caggttgaag aacagttctt cactctgacg
    10321 aatctgctgt cgacatttat gatgcgctac cctggcgcac tgctggcgcg ttaccaccgc
    10381 agtgattttg ctgcgctgtt accgcaccgg acgttaaaag aggcagagag catagccagt
    10441 cagttaatca aagccgtcga taccttgccg aacaataaaa tgctcgatcg cgacgatatg
    10501 atccacattg gtatctgtgc ctggcgtagt ggtcaggata ccgagcaggt aatggaacat
    10561 gcagagtctg ccacgcgtaa tgcgggattg cagggtggca atagctgggc tatttatgat
    10621 gactcgttgc ctgaaaaagg acgcggtaat gttcgctggc gtacgcttat cgagcaaatg
    10681 ctcagtcgcg gcggcccgcg cctttatcaa aaaccggcgg ttactcgcga aggtcaggtt
    10741 catcatcgcg aactcatgtg ccgcatcttc gatggtaatg aagaggttag ctcggcggag
    10801 tatatgccga tggtcttgca gtttggctta tcggaagagt atgaccgtct gcaaatcagc
    10861 cgtcttattc cactattgcg ttactggcca gaggaaaatc tggcgattca ggttaccgtt
    10921 gagtcgctga ttcgtccgcg ttttcagcgt tggctgcgcg atacgttaat gcaatgtgaa
    10981 aaatcacaac gaaaacgcat aattattgaa cttgcagagg ccgatgtagg tcaacatatc
    11041 agtcgtttac aacctgttat tcgtttagtg aatgctttag gggtacgggt agccgtcaac
    11101 caggctggtt tgacgctggt aagcaccagt tggatcaaag aacttaatgt tgagttactc
    11161 aagctccatc cggggctggt caggaacatt gagaagcgaa cggagaacca gctgctggtt
    11221 caaagcctgg tggaagcctg ctccgggacc agcacccacg tttacgccac cggcgtgcgt
    11281 tcgcgaagcg agtggcagac cctgattcag cgcggtgtta caggcgggca aggggatttt
    11341 ttcgcgtcct cacagccact tgatactaac gtgaaaaaat attcacaaag atactcggtt
    11401 taacctgccg tttaatccgt tttcacgtag aataacgcgc gctgcgtctc atgggagtgt
    11461 gcttgtctgc tcgccagatt gttgcagcac atatgcagat gaatgacctt acgcggttgc
    11521 aaacaggcga ggaatgctgc tgatgcatta agcctttctg gactcaggca gagatttgta
    11581 acaaaggaaa cgaactgcac taattttcac cgtagcagat gatttttgcg ccttgtcgct
    11641 gctgcgtgtg gttggtaaag taagcggatt ttcttttccg ccccagcttt caggattatc
    11701 ccttagtatg ttgaaaaaat ttcgtggcat gttttccaat gacttgtcca ttgacctggg
    11761 tactgcgaat accctcattt atgtaaaagg acaaggcatc gtattgaatg agccttccgt
    11821 ggtggccatt cgtcaggatc gtgccggttc accgaaaagc gtagctgcag taggtcatga
    11881 cgcgaagcag atgctgggcc gtacgccggg caatattgct gccattcgcc caatgaaaga
    11941 cggcgttatc gccgacttct tcgtgactga aaaaatgctc cagcacttca tcaaacaagt
    12001 gcacagcaac agctttatgc gtccaagccc gcgcgttctg gtttgtgtac cggttggcgc
    12061 gacccaggtt gaacgccgcg caattcgtga atccgcgcag ggcgctggtg cccgtgaagt
    12121 cttcctgatt gaagaaccga tggctgccgc aattggtgct ggcctgccgg tttctgaagc
    12181 gaccggttct atggtggttg atatcggtgg tggtaccact gaagttgctg ttatctcctt
    12241 gaacggcgtg gtttactcct cttccgtgcg cattggtggt gatcgtttcg acgaagctat
    12301 catcaactat gtgcgtcgta attacggttc tctgatcggt gaagccaccg cagaacgtat
    12361 caagcacgaa atcggttcgg cttacccggg cgatgaagtc cgtgaaatcg aagttcgtgg
    12421 ccgtaacctg gcagaaggtg ttccacgcgg ttttaccctg aactccaacg aaatcctcga
    12481 agcactgcag gaaccgctga ccggtattgt gagcgcggta atggttgcac tggaacagtg
    12541 cccgccggaa ctggcttccg acatctccga gcgcggcatg gtgctcaccg gtggtggcgc
    12601 actgctgcgt aaccttgacc gtttgttaat ggaagaaacc ggcattccag tcgttgttgc
    12661 tgaagacccg ctgacctgtg tggcgcgcgg tggcggcaaa gcgctggaaa tgatcgacat
    12721 gcacggcggc gacctgttca gcgaagagta atcggatgca ggcaggggaa gcgtctgttt
    12781 accctgcctg gtctgatacg agaatacgca taacttatga agccaatttt tagccgtggc
    12841 ccgtcgctac agattcgcct tattctggcg gtgctggtgg cgctcggcat tattattgcc
    12901 gacagccgcc tggggacgtt cagtcaaatc cgtacttata tggataccgc cgtcagtcct
    12961 ttctactttg tttccaatgc tcctcgtgaa ttgctggatg gcatatcgca gacgctggcc
    13021 tcgcgtgacc aactagaact tgaaaaccgg gcgttacgtc aggaactgtt gctgaaaaac
    13081 agtgaactgc tgatgcttgg acaatacaaa caggagaacg cgcgtctgcg cgagctgctg
    13141 ggttccccgc tgcgtcagga tgagcagaaa atggtgactc aggttatctc cacggttaac
    13201 gatccttata gcgatcaagt tgttatcgat aaaggtagcg ttaatggcgt ttatgaaggc
    13261 cagccggtca tcagcgacaa aggtgttgtt ggtcaggtgg tggccgtcgc taaactgacc
    13321 agtcgcgtgc tgctgatttg tgatgcgacc cacgcgctgc caatccaggt gctgcgcaac
    13381 gatatccgcg taattgcagc cggtaacggt tgtacggatg atttgcagct tgagcatctg
    13441 ccagcgaata cggatattcg tgttggtgat gtgctggtga cctccggtct gggcggtcgt
    13501 ttcccggaag gctatccggt cgcggttgtc tcttccgtaa aactcgatac ccagcgcgct
    13561 tatactgtga tccaggcgcg tccgactgca ggactgcaac gtttgcgtta tctgctgctg
    13621 ctgtggggcg cggatcgtaa cggcgctaac ccgatgacgc cggaagaggt gcatcgtgtt
    13681 gctaatgaac gtctgatgca gatgatgccg caggtattgc cttcgccaga cgcgatgggg
    13741 ccaaagttac ctgaaccggc aacggggatc gctcagccga ctccgcagca accggcgaca
    13801 ggaaatgcag ctactgcgcc tgttgcgccg acacagcccg ctgctaatcg ctctccacaa
    13861 agggctacgc cgccgcaaag tggtgctcaa ccgcctgcgc gtgcgccggg agggcaatag
    13921 tggcgagcta tcgtagccag ggacgctggg taatctggct ctctttcctc attgcgctgt
    13981 tgctgcaaat catgccctgg ccggataacc tgattgtttt ccggccaaac tgggtgttac
    14041 ttatcttgtt gtattggatc ctggccttgc cccatcgcgt aaatgtgggc acaggttttg
    14101 tgatgggtgc catactggat ctgatcagcg gctcgatgct tggcgtacgc gtattggcga
    14161 tgagcatcat tgcttacctg gtggcgctga aataccagct tttccgcaac ctcgcattat
    14221 ggcagcaggc gctggtcgtc atgttgcttt cgctggtggt ggatattatt gttttctggg
    14281 cagagttttt agtgattaac gtctctttca gaccggaagt gttctggagt agtgtagtca
    14341 atggggtgct ctggccgtgg attttcttgc tgatgcgtaa agtccgtcag cagtttgccg
    14401 tgcaataaag gtttctatga cttctctgta tttagcttcc ggttctccgc gtcgtcagga
    14461 gttacttgcg caacttggcg tgacctttga acgtattgtt acgggcattg aggagcagcg
    14521 tcagccgcag gagagcgcgc agcagtatgt tgtgcgtctg gcacgcgaga aagcacaggc
    14581 gggtgtcgcg caaacggcac aggatctccc ggtgctgggt gcggatatta tcgttatcct
    14641 gaacggagaa gtgctcgaga aaccgcgcga cgcagagcat gcggcgcaga tgttgcgcaa
    14701 attatcgggt cagacccatc aggtgatgac ggcagtggcg ttggccgaca gccagcatat
    14761 tctcgattgc ctggtggtca ccgatgtgac tttcagaacg ttaacagacg aagacatcgc
    14821 gggctatgtc gccagcggtg aaccgttaga taaagcaggt gcatacggta ttcaggggct
    14881 gggtggctgt tttgtcagga agataaatgg cagctatcac gccgtagtcg gcttaccgct
    14941 ggttgaaacg tatgaattat taagtaattt taacgcactg cgtgagaaaa gggataaaca
    15001 tgacggctga attgttagta aacgtaacgc cttcggaaac gcgagtggcg tatattgatg
    15061 gcggtattct gcaggaaatt catattgaac gtgaggcgcg acgcggaata gtaggcaata
    15121 tctacaaggg tcgtgtaagt cgtgtacttc cgggtatgca ggcggctttt gtagatattg
    15181 ggctggataa agccgcgttt cttcatgcat ccgacatcat gccgcatacc gaatgtgtgg
    15241 cgggtgaaga acaaaagcaa ttcacggtgc gcgacatctc ggaactggtt cgtcaggggc
    15301 aagatctgat ggtgcaggtg gtgaaagatc cgcttggcac taaaggtgcg cgcctgacca
    15361 ccgatatcac gctgccttct cgctatctgg tatttatgcc aggggcttct cacgttgggg
    15421 tttcccaacg aattgaaagc gaatcagaac gtgaacgcct gaaaaaagtg gtcgcagagt
    15481 attgcgacga gcagggcggg tttatcatcc gcactgcagc ggaaggggtt ggcgaggctg
    15541 aactggcctc cgatgccgct tatctgaaac gcgtctggac caaagttatg gagcgtaaaa
    15601 agcgcccgca gacccgttat cagctgtacg gcgaactggc gctggcgcag cgtgttctgc
    15661 gtgattttgc cgacgccgaa ctggaccgca ttcgcgttga ctcacgcctg acttacgaag
    15721 cattgctgga gtttacctcg gagtacattc ccgagatgac cagcaagctg gagcattaca
    15781 ccggacgcca gccgattttc gatctctttg atgtcgaaaa cgaaatccag cgagcgttgg
    15841 aacgcaaagt agaactgaaa tccggtggtt atctgattat cgaccagacc gaagcgatga
    15901 ccaccgtgga catcaatacc ggagcgtttg tcggtcatcg caatctggac gacactattt
    15961 tcaataccaa tattgaagcg acgcaggcta tcgctcgcca gttacggttg cgtaatctgg
    16021 gcgggattat cattattgat ttcatcgata tgaataatga agatcaccgc cgccgcgtgc
    16081 tgcattcgct ggagcaggcg ttgagcaaag accgggtgaa aaccagcgtt aatggttttt
    16141 cggcgctggg gctggtggag atgacgcgta aacgcacccg cgaaagcatt gagcacgtac
    16201 tgtgtaacga atgcccaacc tgccacggtc gcggaacggt gaaaaccgtg gaaacggtat
    16261 gctatgaaat catgcgcgag attgttcgtg tccaccatgc ttacgactcc gaccgtttcc
    16321 tggtctatgc ttctccggca gtagccgaag ccttgaaagg cgaagagtca cactcgctgg
    16381 cggaagtgga aattttcgtt ggcaaacagg ttaaagtaca aattgaaccg ctctataacc
    16441 aggagcagtt tgacgtcgta atgatgtaaa cagatgctgg gccgccatcc ggcaaagggt
    16501 ttttgagtca catttttagc agacaaggag tgacgggtga ggcgattgcc ggggatttta
    16561 ctgcttactg gagccgcgct cgttgtgatc gctgccctgc tggttagcgg cctgcgtatt
    16621 gctttaccgc atcttgacgc ctggcgtccg gaaatcctca acaaaataga atccgcgact
    16681 ggcatgccgg tagaagccag tcagctctca gccagctggc agaattttgg cccgacgctt
    16741 gaagcacacg atatccgtgc agaactaaaa gatggcggcg aattttcggt taaacgcgtt
    16801 actctggcgc tggatgtctg gcagagcctg ttacatatgc gctggcagtt tcgcgacctc
    16861 actttctggc agctgcgctt tcgcaccaac actcctatca ccagcggtgg tagcgatgac
    16921 agtctggaag ccagtcacat cagcgatctg tttcttcgtc aatttgacca tttcgatctc
    16981 cgcgacagtg aagtcagttt tctgacgcca tccggtcaga gcgccgagct ggcgatccca
    17041 caactcacct ggctgaacga tccacgtcga caccgtgcgg aaggcctggt aagcctctcc
    17101 agccttaccg ggcagcacgg cgtgatgcag gtgcgcatgg atttgcgcga tgatgagggg
    17161 ttgttaagca atggtcgcgt ctggctacag gcggatgaca tcgacctgaa gccgtggctc
    17221 ggtaaatgga tgcaggacaa tattgctctg gaaacggcac agttctccct tgaaggctgg
    17281 atgacgatcg acaaaggcga tgtaaccggc ggtgacgtct ggctgaaaca gggcggtgcc
    17341 agctggttgg gcgagaagca aacgcatacg ctgtcggtgg ataatctgac cgcgcatatt
    17401 acgcgtgaaa atccgggctg gcagttctct attcccgata cacggatcac gatggacggc
    17461 aaaccctggc cgagcggagc attgacgctg gcctggatac cggaacagga cgttgacggc
    17521 aaagacaata aacgcagtga cgaactccgg attcgcgcta gtaatctgga gctggcaggc
    17581 ctggagggca tacgcccgct ggccgcgaaa ctttcacctg cactgggtga tgtttggcgc
    17641 tccacacaac cgagcggcaa gattaacact ctggcgctgg atatcccgct tcaggcggca
    17701 gacaagaccc gttttcaggc atcgtggagc gatctggcct ggaagcaatg gaaattatta
    17761 ccgggtgcgg aacacttctc cgggacgctt tccggcagcg ttgaaaatgg tttgcttacc
    17821 gcgtcgatga agcaggcaaa gatgccttac gaaacggtat tccgtgcgcc actggaaatc
    17881 gccgacggcc aggcaactat aagctggctg aacaatgaca aaggtttcca gctggatggg
    17941 cgtaatattg acgttaaagc caaagccgtc catgcgcgcg gcggttttcg ttacctgcaa
    18001 cctgctaacg atgaaccctg gctgggtatt ctggctggca tcagtaccga tgatggttca
    18061 caagcctggc gctatttccc ggaaaacttg atgggtaaag acctggttga ttatttaagt
    18121 ggtgcgattc agggcggtga agcggataac gcgacgctgg tttatggtgg caatccgcaa
    18181 ctcttcccct ataaacacaa cgaaggtcag tttgaagtgc tggtgccgct gcgcaacgcg
    18241 aagtttgcct tccagccgga ctggcctgca ttaactaacc ttgatattga actggacttt
    18301 attaacgacg gtttatggat gaaaaccgat ggcgttaatc tgggcggcgt gcgcgcgagt
    18361 aatctcaccg cagtgatccc tgactactca aaagaaaaac tgctgattga cgctgacatt
    18421 aaaggtccgg gtaaagccgt tggcccttac tttgatgaga caccgctgaa agattctctg
    18481 ggtgcgaccc tgcaagaact ccagctcgac ggcgatgtga atgctcgctt acatcttgat
    18541 atcccgctga acggcgaact ggtaaccgcg aaaggtgaag tgacgctgcg taataacagt
    18601 ctgtttatca aaccactcga cagcatcctg aaaaatttga gcggtaaatt cagctttatc
    18661 aatggcgatc tgcaaagtga accactgaca gcaagctggt ttaatcagcc gttgaacgtg
    18721 gatttttcca ccaaagaagg ggcaaaagcc taccaggttg cggtgaatct caacggtaac
    18781 tggcaaccgg cgaaaaccgg cgttctgcct gaagcggtga acgaagcatt gagtggcagc
    18841 gtggcgtggg atggtaaagt gggcattgat ctgccttatc atgctggcgc gacctataac
    18901 gtagagctga acggcgatct gaataatgtg agtagtcact taccttcacc gttagccaaa
    18961 cctgcgggtg aaccactagc ggtaaacgtt aaggttgatg gcaatctcaa cagctttgaa
    19021 ttaaccggac aggctggtgc ggataaccat ttcaatagcc gctggttgct tggtcaaaag
    19081 ctgacgctcg atcgtgctat ttgggcggca gacagtaaaa cgctcccgcc gttgccggaa
    19141 caaagtggcg ttgaacttaa tatgccgccg atgaatggtg ccgagtggct ggccctgttc
    19201 cagaaaggcg ctgcggagag tgtcggtggt gcagcgagtt tcccacaaca cataacatta
    19261 cgtacgccta tgttgtcact gggaaatcag caatggaata acctgagtat tgtttcgcaa
    19321 ccgacggcaa atggcaccct ggttgaggcg caagggcgtg aaatcaacgc cacgctagcg
    19381 atgcgtaata acgcgccgtg gctggcgaat atcaaatatc tttattacaa cccgagcgtg
    19441 gcgaaagctc gtggtgattc aacgccgtca tcacctttcc cgacaacgga gcgcattaac
    19501 ttccgtggct ggccggacgc acaaatacga tgcacagagt gctggttctg ggggcaaaaa
    19561 ttcggtcgca ttgacagtga tatcaccatt tctggcgata cgttaacgct gaccaatgga
    19621 ctgattgata ctggtttctc gcggcttact gccgatggtg aatgggttaa taatccgggg
    19681 aatgaacgta cctcgctgaa aggaaaactg cgtgggcaga aaattgatgc cgccgcagaa
    19741 ttttttggtg tcacgacgcc catacgccag tcgtcattta atgtggatta cgatttacac
    19801 tggcgcaaag caccctggca gccagatgaa gcgacgttga atggcatcat tcatactcaa
    19861 ctgggtaaag gcgaaattac cgaaatcaat accggacatg ccgggcaatt gctgcgctta
    19921 ttgagcgtag atgccctgat gcgtaagctg cgttttgatt tcagagacac ttttggcgaa
    19981 gggttctatt ttgactccat tcgcagcacc gcgtggatta aagacggcgt tatgcacacc
    20041 gacgacacgc tggtggatgg cctggaggcg gatatcgcca tgaaagggtc ggtaaatctg
    20101 gtacgtcgag acctgaatat ggaagcggtt gtcgcaccag agatttctgc gacggtgggc
    20161 gtggctgcgg cttttgcggt taaccccatt gttggcgcgg cagtgtttgc cgccagtaaa
    20221 gtgctggggc cgctgtggag caaagtctcc attttgcgct atcacatttc gggtccgctg
    20281 gatgatccgc aaatcaacga agtgttgcgc caaccgcgta aagaaaaagc gcaatgattt
    20341 gacgagggcg cgtaattgcc ccaatctcat atgataatcg ttgccaaagg ccaacgagcc
    20401 ggaacataac tgtaggccgg atagggcgtt cacgccgcat ccggcagccg taaaaaatcc
    20461 tctactgcag taactaacga gtagcaaaaa cgatgagtct gaacctggta agtgaacaat
    20521 tgctagcggc gaacggcctg aaacatcagg acttgttcgc gatcctcggt caactggccg
    20581 aacgtcgcct tgattatggc gatctctatt ttcagtcgag ctatcacgaa tcctgggttt
    20641 tagaagaccg cattattaaa gatggttctt acaacatcga tcagggcgtt ggcgtgcgtg
    20701 caatcagcgg tgaaaaaacc ggatttgctt acgctgacca aatcagcctg ctggcgctgg
    20761 aacagagtgc gcaagcggcg cgcaccatcg tccgtgatag tggtgatggc aaagtacaga
    20821 cgctgggcgc ggtagagcat agcccgttgt atacctcggt agatccgctg caaagcatga
    20881 gccgtgaaga gaagctggat atcctgcgtc gcgtcgataa ggttgctcgc gaagcggaca
    20941 agcgcgtaca ggaagtgact gccagcctca gtggtgtcta tgaattaatt ctggttgcgg
    21001 ccaccgacgg cacgctagcg gcggatgtcc gtccgctggt gcgtctttcc gtgagcgttc
    21061 tggtcgaaga agatggcaaa cgcgaacgcg gtgccagtgg cggcggcggt cgttttagtt
    21121 atgagttctt ccttgccgat cttgacggcg aagtccgtgc ggatgcatgg gcaaaagaag
    21181 cagtgcgtat ggcgctggtc aatctttctg ccgttgctgc accagcgggc accatgccgg
    21241 tagtacttgg cgcaggttgg ccgggcgtgc tgttgcatga agcggtaggt cacggtctgg
    21301 aaggcgactt caaccgccgt ggcacttcag tatttagtgg acaggtcggg gagctggtgg
    21361 cttcagaact gtgtaccgtg gttgatgacg gcacgatggt cgatcgccgt ggttcggtgg
    21421 cgattgatga cgaaggtacg ccaggccagt acaacgtgct gattgagaac ggcattctga
    21481 aaggctacat gcaggataaa ctcaacgcgc gtttgatggg gatgacgccg actggcaacg
    21541 gtcgccgtga atcctacgcc catctgccca tgccgcgtat gaccaacacc tatatgctgc
    21601 cgggtaaatc gaccccgcaa gaaattattg aatccgttga gtacggtatc tatgcaccga
    21661 actttggtgg cggtcaggtg gatatcacct ccggcaaatt cgttttctcc acttcagaag
    21721 catatctgat tgaaaacggt aaagtaacga agccggtgaa aggcgcaacg ttgattggtt
    21781 ccggtatcga aaccatgcag cagatttcga tggttggcaa cgacctgaaa ctggataacg
    21841 gcgtgggtgt ctgcggtaaa gaagggcaaa gtttgccggt tggcgtgggc cagccaacgt
    21901 tgaaagtcga taacctgact gttggcggta ctgcgtaata atttcattat tttcagaagg
    21961 ataattaatc tttctacgtg cacgaacggt cccctcgctc ctctggggtt agggtgaggg
    22021 gaacccgttg gcacaggttt gtagaacgta acagtacaat atgaattact tctctttccc
    22081 gcgcccgtgc atctcctgaa acaatttacc gacctcaaca aaataatccg tcagcgagtt
    22141 gatcacgacc tgtaccttca gcggcagctt atctttttcg gtatataacg cataaaccgg
    22201 gcgtggatct gactgataac gcggcagcag gatctccagc tccccacgat tgatctcgtt
    22261 gatcacccac atcagcggca cgtaggcgat cccggcaccc gccgttagcc agcgcaccag
    22321 cgtcatcgga tcattagtca caaatctccc ttgcgggatc aggcgagtcg agatcccttc
    22381 cggtgcaatc agttcaaatt cattgtcggg ccgcacgctg tattcaagcc atgaatggct
    22441 acttaaatcg gcgggttttt ccggtatgcc gtattgtgtg agatagcttt tcgcggcgca
    22501 caccaccatt ggcatcgcgc caagacggcg ggaaaacagg ctggaatcct gcaacgcgcc
    22561 gacgcggatc accacatcca gaccgtcggc aatcaggtcg ggggctggaa ttccggtaac
    22621 cagattggcg ctcaaacctg ggtattcttt cagcattttg gctgtcagcc cggcgagaac
    22681 attttgtgcc atagttgaag aacagccaat gcgtagcgtc ccgatggggg tgttattgaa
    22741 ggcatacagt tgctcatgaa catcctgcac ttcatgaagc atacgacggc agccctggta
    22801 gtaaattcta ccggcttcgg tcaggccaat gctgcgtgtg ctacggttta acagctttac
    22861 ctgcaactca tcttccagtt ttgacaccgt ctgactgatg gacgaaacgc tcatctgtag
    22921 ctgtctggcg gcggcggtaa aagagccaaa ttcaactact ttggcaaaca ccgacatgcg
    22981 ttttagtcgt tccattattc actctgactt aaaagtgatt tagatcacat aatatagata
    23041 acagcataac agttacgcta atatattaaa tatcaatcta cagcaatgtt gctctcgccc
    23101 ggctttccat gcccttctct gcggcgacag atgctgaaaa taataacgcc tgctctctct
    23161 atacaaccaa ggtcaacatg agtctgtttc ccgttatcgt ggtgtttggg ctgtccttcc
    23221 caccgatatt ttttgaattg cttttatcac tggcgatttt ctggctggtg cgccgggtac
    23281 ttgtgccaac aggtatctac gactttgtct ggcatccggc gttgttcaac accgcgctct
    23341 attgctgctt gttttatttg atatcgcgac tgttcgtttg aggttgaagt gaaaacacta
    23401 ataagaaaat tctcccgtac ggccatcacg gtcgtattag tcattctggc cttcatcgca
    23461 atttttaatg cctgggtcta ttacaccgaa tccccctgga cgcgtgacgc gcgctttagc
    23521 gctgacgtcg ttgcgatcgc gccggacgtt tctggactca ttacccaggt gaatgttcat
    23581 gataaccagc tggtgaaaaa aggacagata ctgttcacca tcgaccagcc gcgctatcaa
    23641 aaggcgcttg aggaagcgca agccgatgtt gcttattatc aggtactggc acaggagaaa
    23701 cgccaggagg ccggacgtcg taaccgtctc ggtgtgcagg cgatgtctcg cgaagagatc
    23761 gaccaggcta acaacgtact acaaacggtt ctgcatcagt tagcgaaagc gcaggcgacc
    23821 cgcgatctgg caaaactgga tcttgaacgc acggtgatcc gagcgccagc agatggctgg
    23881 gtgaccaacc tcaacgtcta taccggtgag tttattactc gaggatcaac ggcggttgcg
    23941 ctggtgaaac agaactcctt ctatgtactg gcctatatgg aagaaactaa gctggaaggg
    24001 gtgcgtccgg ggtatcgtgc agagatcacg ccgcttggca gtaacaaagt gctgaaaggg
    24061 actgttgata gtgttgccgc tggggtcacc aacgccagca gcacgcgtga cgacaaaggg
    24121 atggcgacca tcgactctaa ccttgaatgg gtgcgtttgg cgcaacgtgt tccggttcgt
    24181 attcgtctcg acaaccagca agagaacatc tggcctgcgg gcaccactgc tacagtggtg
    24241 gtcactggca aacaagatcg cgacgaaagc caggattcgt tcttccgtaa aatggcccat
    24301 cgcctgcgtg agtttggtta atcacgatgg gtattttctc cattgctaac caacatattc
    24361 gctttgcggt aaaactggcg accgccattg tactggcgct gtttgttggc tttcacttcc
    24421 agctggaaac gccacgctgg gcggtactga cagcggcgat tgttgccgcc ggtccggcct
    24481 ttgctgcggg aggtgaaccg tattctggcg ctattcgcta tcgtggcttt ttgcgcatca
    24541 tcggcacatt tattggctgt attgccggac tggtgatcat cattgcgatg atccgcgcac
    24601 cattattgat gattctggtg tgctgtatct gggccggttt ttgtacctgg atatcctcgc
    24661 tggtacgaat agaaaactcg tacgcgtggg ggctggccgg ttataccgcg ctgatcattg
    24721 tgatcaccat tcagccggaa ccattgctta cgccgcagtt tgccgtcgaa cgttgtagcg
    24781 agatcgttat cggtattgtg tgtgcgatta tggcggattt gctcttttct ccgcgatcga
    24841 tcaaacaaga agtagatcga gagctggaaa gtttgctggt cgcgcaatat caattaatgc
    24901 aactctgtat caagcatggc gatggtgaag ttgtcgataa agcctggggc gacctggtgc
    24961 gacgcaccac ggcactacaa ggcatgcgca gcaacctgaa tatggaatct tcccgctggg
    25021 cgcgggccaa tcgacgttta aaagcgatca atacgctatc gctgacgctg attacccaat
    25081 cctgcgaaac ttatcttatt cagaatacgc gcccggaatt gatcactgat actttccgcg
    25141 aattttttga cacgccggta gaaaccgcgc aggacgtcca caagcagctc aaacgcctgc
    25201 ggagagttat cgcctggacc ggggaacggg aaacgcctgt caccatttat agctgggtcg
    25261 cggcggcaac gcgttatcag cttctcaagc gcggcgttat cagtaacaca aaaatcaacg
    25321 ccaccgaaga agagatcctg caaggcgaac cggaagtaaa agtagagtca gccgaacgtc
    25381 atcatgcaat ggttaacttc tggcgaacca cactttcctg cattctgggc acgcttttct
    25441 ggctgtggac gggctggact tccggcagtg gtgcaatggt gatgattgcg gtcgtgacgt
    25501 cactggcaat gcgtttgccg aatccacgca tggtggcgat cgactttatc tatgggacgc
    25561 tggccgcgct gccgttaggg ctgctctact ttttggtgat tatccctaat acccaacaga
    25621 gcatgttgct gctgtgtatt agcctggcag tgctgggatt cttcctcggt atagaagtac
    25681 agaaacggcg actgggctcg atgggggcac tggccagcac cataaatatt atcgtgctgg
    25741 ataacccgat gactttccat ttcagtcagt ttctcgacag cgcattaggg caaatcgtcg
    25801 gctgtgtgct cgcgttcacc gttattttgc tggtgcggga taaatcgcgc gacaggactg
    25861 gacgtgtact gcttaatcag tttgtttctg ccgctgtttc cgcgatgact accaatgtgg
    25921 cacgtcgtaa agagaaccac ctcccggcac tttatcagca gctgtttttg ctgatgaata
    25981 agttcccagg ggatttgccg aaatttcgcc tggcgctgac gatgattatc gcgcaccagc
    26041 gcctgcgtga tgcgccgatc ccggttaacg aggatttatc ggcgtttcac cgacaaatgc
    26101 gccgcacagc agaccatgtg atatctgccc gtagcgatga taaacgtcgt cggtactttg
    26161 gccagttgct ggaagaactg gaaatctacc aggaaaagct acgcatctgg caagcgccac
    26221 cgcaggtgac ggaaccggtt catcggctgg cggggatgct ccataagtat caacatgcgt
    26281 tgaccgatag ttaagtcaaa accgacgcca aaagcgtcgg ttttttcatg gctatactta
    26341 gcgatgcacg gcagaactcg ccgcgaaacg tgacggtggc aacagatgaa tatttatgcc
    26401 tttgatttta atgagattga gagtcaggag gatttttatc gtgactttag ccaaaccctt
    26461 ggtctggcta aagataaggt acgcgatctt gactcactat gggatgtgtt aatgaacgat
    26521 gtcctgccgc taccacttga gattgaattt gttcatctgg gagagaaaac gcgtcgccgt
    26581 tttggcgcgt taatattgct gtttgatgag gcagaggaag agctggaagg gcatttgcgt
    26641 tttaatgttc gtcattagcg caaaaaaaag cccccgaacc gggggcaata tcgtcggaca
    26701 aaacgatgag ggtttatttg tacagttcag ccgtagcgtg ccaggtgtca ccgctacgag
    26761 cttcagtaat ctggtaggcc gttgcgcctt tctcttccgc ttttttgttc agcatttcac
    26821 gtatatccat tggcgaagac gccacaccac ttacggatac ggtcccgatt gcttcacgat
    26881 tttgtgcttg tgcagcatca atggagtcgg cagcgaatgc accgaaagag agaacagaaa
    26941 gtacgcttaa tgcagctaca gtggttttga ttttcatgat ttttacctcg acataatctt
    27001 ttagctgggc ctttgtttcg tgacccatat cacaaaatca agcatacact aattattgaa
    27061 ctaattaata ccacgctaac tgtttcagtt gatactaagt gttaaaaatg tgaattttaa
    27121 taacaaaaaa gtataaaaaa cacggttatt tattattaat tatagtattt tcaattagat
    27181 aatgaatttt gcataatgtg ctttttttac cgtatgtatt cattttgtga atgatgtgtc
    27241 gcggtaaaac gcactattcg ttaaatgtat cgcaggagaa agaaggggaa tgagagggat
    27301 aagcaacatt ttccccgccg ccagaagcga cggggcagag attaaagctc ctggtcgaac
    27361 agctctaaaa tcgcttcgta caggtctttg acggtgaaac cgttagcagg ggtggtaaag
    27421 atggtgtcat cgccagcgat ggtgcccaga ataccttctg ctttgcccag tgagtccagc
    27481 aggcgagcaa ttaactgcgc cgcgccaggg ctggtatgaa tcacgacaac tgcatcgttg
    27541 tagtcgatat ccagcaccag attcttcaat ggactggagg tggttggtac acccagttca
    27601 gctggcaggc agtaaaccat ttccattttg gcattgcgtg tacgtacagc accaaacttg
    27661 gtcaacatcc gcgagacttt agactgatta atattgtcaa agccttgctc ctgcaacgcg
    27721 gcgacgattt cgccctggga gctaaatttc tcttctttaa gtaatgcttt aaatgcttta
    27781 actagttctt cttgcttagc cgagcttcgc ataagtcacc cgatatggtg gttgatacaa
    27841 cattattgtg catacagatg aatttttatg caaacagtca gccctgaaga aggctgaaat
    27901 aatgttatga aagagcggga ttttatcaaa tttcgttatt gagaaacatg cctgcgtcac
    27961 ggcatgcaaa ttctgtttaa aagtaaatta attgttatca aattgatgtt gttttggctg
    28021 aacggtaggg tatattgtca ccacctgttg gaatgttgcg ctaatgcata agcgactgtt
    28081 aattacgtaa gttaggttcc tgattacggc aattaaatgc ataaacgcta aacttgcgtg
    28141 actacacatt cttgagatgt ggtcattgta aacggcaatt ttgtggatta aggtcgcggc
    28201 agcggagcaa catatcttag tttatcaata taataaggag tttaggatga aagtcgcagt
    28261 cctcggcgct gctggcggta ttggccaggc gcttgcacta ctgttaaaaa cccaactgcc
    28321 ttcaggttca gaactctctc tgtatgatat cgctccagtg actcccggtg tggccgtcga
    28381 tctgagccat atccctactg ctgtgaaaat caaaggtttt tctggtgaag atgcgactcc
    28441 ggcgctggaa ggcgcagatg tcgttcttat ctctgcaggc gtagcgcgta aaccgggtat
    28501 ggatcgttcc gacctgttta acgttaacgc cggcatcgtg aaaaacctgg tacagcaagt
    28561 tgcgaaaaac tgcccgaaag cgtgcattgg tattatcact aacccggtta acaccacagt
    28621 tgcaattgct gctgaagtgc tgaaaaaagc cggtgtttat gacaaaaaca aactgttcgg
    28681 cgttaccacg ctggatatca ttcgttccaa cacctttgtt gcggaactga aaggcaaaca
    28741 gccaggcgaa gttgaagtgc cggttattgg cggtcactct ggtgttacca ttctgccgct
    28801 gctgtcacag gttcctggcg ttagttttac cgagcaggaa gtggctgatc tgaccaaacg
    28861 tatccagaac gcgggtactg aggtggttga agcgaaagcc ggtggcgggt ctgcaaccct
    28921 gtctatgggc caggcagctg cacgttttgg tctgtctctg gttcgtgcac tgcagggcga
    28981 acaaggcgtt gtcgaatgtg cctacgttga aggcgacggt cagtacgccc gtttcttctc
    29041 tcaaccgctg ctgctgggta aaaacggcgt ggaagagcgt aaatctatcg gtaccctgag
    29101 cgcatttgaa cagaacgcgc tggaaggtat gctggatacg ctcaagaaag atatcgccct
    29161 gggcgaagag ttcgttaata agtaattgat tagcgaataa taaaaaaccg gagcacagac
    29221 tccggttttt gttttgagca ctcgacttaa ttggttgccg gatattcctg aatggtgacc
    29281 tgcagcgtta actgcttatc atcacgcatc actacaaccg ggatcaccga accagggcga
    29341 atttccgcca cctgatccat cgtctccaga gcagagatgg ccggtttgtt atccaccgaa
    29401 ataatcagat cgttgacctg aatacccgca ttcgccgctg ggccgtcagg tgacacttca
    29461 ttaaccacga tcccttgcag ttgatctata ccaccgctct gcgcgtgcag tggtgcgatc
    29521 tcgcgtccgc cgataccaat gtagccgcgg atcacgcgac catcgcggat cagcttatcc
    29581 ataattttgg ttgctaactg gaaaggaatc gcaaagccga taccttccgg cgtttcgcca
    29641 tcgttactct tatcaaacga cagcgtgtta atgcccatca gttctcccag cgagttcacc
    29701 agcgcgccgc cagagttacc gtggttaatg gaagcatcgg tttgtaggaa gttttgccgc
    29761 ccggtcgggt tcagaccgat tcgacccgtg gcactaataa tcccttgggt aatggtctgc
    29821 ccgaggttgt acgggttacc gatcgccagt actacgtcgc caatgtgcgg tacgcgacgt
    29881 gcattaattg gaatggtagg taaaccgcca gtggcattaa ttttaagtac cgccagatcg
    29941 gttagagagt cagatcccac cagcaatgct tcaaatacac gtccatcctg taaggcgacg
    30001 atgatctgat cggcgtcgtt gatgacgtgt ttattggtga tgatataacc gcgttgatcc
    30061 atgattacac cggatcccag ggtgcggatc tcaagctggt tgtgagagtt ggtgttcaaa
    30121 ccacggttgt aaacgttaac caccgctggc gcggcgcggc gaaccgccag attatagctg
    30181 gcaggcgtct catcggtact gtcaaattgc ggagtggaaa gcgggttaag gctgcgcagc
    30241 gaaggcatgg caaccagcag aatagcgccg acaattaatc caatcgcaac ggaacgtaag
    30301 agcttcacaa acatgatgga ggcgtcatta aaaaagggaa cggcagcagc ataccacgag
    30361 ttaaccggac atcacacgtc agcctgatgc ccggtttacg acattaacgc atcagcagat
    30421 agatgctttc attgccgcgt acaatttgca gggcgatgat ggccggtttt gccgccagca
    30481 ctttacgcat ttcagcaatc gagttcaccc gatcgcggtt gacgccaatg atcacatcgt
    30541 ctttttgcaa gccagcctga gcagctgggc ttcctttgac aacttcatct atcttaatac
    30601 ctttgccgcc atcttttagc tgaccatcgc tcaacgttgc accttccagc gctggcgtga
    30661 tcatttcagc gctggccgac gaagaggtgt tggtatcaag cgtcacttct acttccagtg
    30721 gtttgccgtt acgcagcagg ccaagcttca ctttcgtgcc cggctctgta gtcgcgatac
    30781 gagagcgcaa ctcagcaaag ctattcagcg gtttgccgtt gaggctggta ataatatcgc
    30841 ccgctttgac gcccgctttc gctgagccag aacttggcaa cacttcgctg acaaacgcgc
    30901 cacgctgcac gtcaaggttg aaggctttgg cgatatcggc actcatctcg gtgcctttga
    30961 tgcctaacaa accgcgtttg atttcaccaa agtcgataag ctgctgcgcc agtgttcgcg
    31021 ccatattact ggggatggca aatccaatcc cgacgctccc gccgccaggc gcaaggattg
    31081 cagtgttgat gccaattaac tcaccgttaa ggtttaatag tgcaccgccg gagttaccgc
    31141 ggttaatgga agcatctgtc tggataaagt tttccagacc ttcaagattc aacccgctgc
    31201 ggcctaatgc ggaaacaatg ccagaggtgg cggtttgccc aaggccaaat gggttaccga
    31261 ccgctacggc aaaatcaccg acgcgcaatt tatcggagtc ggcaatagcg atttgcgtta
    31321 atttgctcgg gttttgaatt tgtaacaggg cgatatcgct ctggtcatcg ctaccaatca
    31381 gtttttcatc aaactcgcgc ccatcattga gctgaatact gattttctgt gcctgattaa
    31441 tcacatggtt gttggtcagc acatagcctt tactggcgtt gatgatgaca ccggaaccta
    31501 aaccttcgaa gggttgtgca ggttgatccg gtaaatcatc accaaaaaac tttttgaatt
    31561 cttccgggat tttctgtccc tgactggccg ttccttccac ccgtacgctc accactgccg
    31621 gaagcacttt ttccagcatt ggagccagac tggggagagg ggcctgatcg gcaacctggc
    31681 ctggaatcga cgcgacggcc tgaaatgacg ccgagagagt taacccggca cttaacgcta
    31741 atgcactcaa cagctgggtt tgttttttca ttattcctgc tctcgtacct gaatgataag
    31801 aaaagagatt caaaacagtt tgatgttatt gaattttcag acggctaaca atgagacacc
    31861 agattaaatg atagtcgtgg agggagagaa gaggagggcg caatggctgc gcccgaaaaa
    31921 taaattagtc gcgcttcgcg ccagtacgca gcaggccgga tgcgccttca gaatagtcgc
    31981 gaggcatctg caccggtgcc tgatcgttgc tggcttcaga ctctgccaga cgattacgga
    32041 acgggtttgc ttcagcagac agttccggca gcaggctgct ggagcttttt gccatgtgct
    32101 gatacagctg gcgatagtcg tgcgccatgg tatccagtaa ttccgcgctg cgggcaaagt
    32161 ggctaaccag ctcttcgcga tactcgtcca gttcagcttt attcttttcc agttcgtact
    32221 gcaacgcctg ttgctggcgt agtttacgat taccaaaacg catggccaca gcaccaataa
    32281 tgatgccgac gactaaccca attagcgcat attcccaggt catgaacatc tcccgttgtc
    32341 ttttgtttcc gtagggtgtt ggcttcaggc tccatgcctg cggctgatta tgccactata
    32401 accgttaatt ccacagaagt ggaatcccga ctgcatatcg cgtagtgtag aacggccttt
    32461 ttttcgtcaa ccgtgaacaa cggcgcaccg attattcaag gaataacaat aagatcatgc
    32521 aaagcgttac cccaacatcg caatacctga aggcgctcaa tgaaggcagc catcaacccg
    32581 acgacgttca aaaagaggcc gtcagccgcc tggaaattat ttatcaagaa ctcatcaata
    32641 gcaggccacc agcccccagg acgagtgggc taatggcgcg ggtcggtaag ctgtggggta
    32701 aacgcgaaga cacaaagcat acgccagtgc gtggcttata tatgtggggc ggtgtaggac
    32761 gcgggaaaac ctggctgatg gaccttttct atcaaagcct gccgggagag cggaaacagc
    32821 gcctgcactt tcaccgtttt atgctgcggg tgcacgaaga gctaactgcc ttacaggggc
    32881 agaccgatcc gctggaaatt attgccgatc gctttaaagc cgaaactgac gtgctctgtt
    32941 ttgacgaatt ttttgtttct gatattaccg acgccatgct acttggcggt ctgatgaaag
    33001 ccctgttcac ccgcggtatt accctggtag cgacgtcaaa tattccgccg gacgaacttt
    33061 atcgaaatgg cctgcaacgt gcgcgttttc tgcctgcaat cgatgccatt aaacagcatt
    33121 gtgatgtaat gaacgtggac gctggtgttg attatcgact gcgtacactc actcaggcgc
    33181 atctgtggct ttcgccactc aacgatgaaa cccgggcgca gatggataaa ctatggttgg
    33241 cgctggcggg ggtgaaacga gaaaattcac cgacgttaga aatcaaccat cggccattgg
    33301 cgacaatggg cgtcgagaac cagacgctgg cggtttcttt tactacgctg tgcgtcgacg
    33361 cccgcagtca gcatgactat attgcgctct cacgcctgtt tcacacagta atgttgtttg
    33421 atgtgccagt catgacgcgg ttgatggaga gcgaagcgcg gcgctttatt gcgctggtgg
    33481 atgagtttta cgagcgccat gtcaaattag tggtgagtgc agaagtgccg ctgtatgaaa
    33541 tttatcaggg tgagcggctg aagtttgagt tccagcgttg cctgtcacgt ctgcaagaga
    33601 tgcaaagcga agagtatctg aagcgcgagc atttggcggg ttaaaacctg ccacaaatca
    33661 caaaaagggg tcgatctttg accccgactt ctctataatc ctgcgacccc acgttacaag
    33721 aaagtttttt tcccaaaact ttttgtgtgc tggcataggc tattcgaagg ggtaggtttg
    33781 ccggactttg tcgtgtgaac ctcaacaatt gaagacgttt gggtgttcac caacgtgtaa
    33841 ctatttattg ggtaagcttt taatgaaaac ttttacagct aaaccagaaa ccgtaaaacg
    33901 cgactggtat gttgttgacg cgaccggtaa aactctgggc cgtctggcta ctgaactggc
    33961 tcgtcgcctg cgtggtaagc acaaagcgga atatactccg cacgtagata ccggtgatta
    34021 catcatcgtt ctgaacgctg acaaagttgc tgtaaccggc aacaagcgta ctgacaaagt
    34081 gtactatcac cacaccggcc acatcggtgg tatcaaacaa gcgacctttg aagagatgat
    34141 tgctcgccgt cctgagcgtg tgattgaaat cgcggttaaa ggcatgttgc caaaaggccc
    34201 gctgggtcgt gctatgttcc gtaaactgaa agtttacgcg ggtaacgagc acaaccacgc
    34261 ggcacagcaa ccgcaagttc ttgacatcta atcgggatta taggcaatgg ctgaaaatca
    34321 atactacggc actggtcgcc gcaaaagttc cgcagctcgc gttttcatca aaccgggcaa
    34381 cggtaaaatc gtaatcaacc aacgttctct ggaacagtac ttcggtcgtg aaactgcccg
    34441 catggtagtt cgtcagccgc tggaactggt cgacatggtt gagaaactgg acctgtacat
    34501 caccgttaaa ggtggtggta tctctggtca ggctggtgcg atccgtcacg gtatcacccg
    34561 cgctctgatg gaatacgacg agtccctgcg ttctgaactg cgtaaagctg gcttcgttac
    34621 tcgtgacgct cgtcaggttg aacgtaagaa agtcggtctg cgtaaagcac gtcgtcgtcc
    34681 gcagttctcc aaacgttaat tggcttctgc tccggcagaa aacaatttgc gaaaaaaccc
    34741 gcttcggcgg gtttttttat ggctaaattc tgaatcagcg taaaaaatgg gaagttgctt
    34801 tttgctgccg tctgaaagac aggtaaaaca aaaaccacat cgccaataag ggtctaagtc
    34861 aactatttca gactaaagcg catctctttt tccccatttc cggcatccac tcaccacaaa
    34921 ggtcgcaaaa tctggtaaac tatcatccaa ttttctgccc aaatgtcggg tattgctcat
    34981 tttttgtttg attttcgaac aaagagagtc gttccttatt gggtaacaca acttctgact
    35041 ggccacctgg tggctggtag cagtaaaaat tctgactata cctggaggtt ttcatggctg
    35101 tcgctgccaa caaacgttcg gtaatgacgc tgttttccgg tcctactgac atctatagcc
    35161 atcaggtccg cattgtgctg gctgagaaag gtgtaagttt cgagatcgaa cacgtggaaa
    35221 aggacaatcc gcctcaggat ctgattgacc ttaacccgaa tcagagcgtt ccgaccctgg
    35281 tggatcgtga gctgaccctg tgggaatctc gcatcattat ggaatatctg gatgagcgtt
    35341 tcccgcatcc gccactgatg cctgtttacc cggtagctcg cggtgaaagc cgtctgtaca
    35401 tgcatcgcat cgaaaaagac tggtacacgc tgataaacac tatcatcaac ggttcagctt
    35461 ctgaagcaga tgccgcacgt aagcaactgc gcgaagaact gctggcgatt gcgccggtct
    35521 tcggtcagaa gccgtacttc ctgagcgatg agttcagcct ggtcgattgc taccttgctc
    35581 cgctgctgtg gcgtctgccg caactgggca tcgagttcag cggcccgggt gcgaaagagc
    35641 tgaaaggcta tatgacccgc gtctttgagc gtgactcttt ccttgcttct ttaactgaag
    35701 cagaacgtga aatgcgtctg ggccggagtt aatctgtatg gatttgtcac agctaacacc
    35761 acgtcgtccc tatctgctgc gtgcattcta tgagtggttg ctggataacc agctcacgcc
    35821 gcacttggtg gtggatgtga cgctccctgg cgtgcaggtt cctatggaat atgcgcgtga
    35881 cgggcaaatc gtactcaaca ttgcgccgcg tgctgtcggc aatctggaac tggcgaatga
    35941 tgaggtgcgc tttaacgcgc gctttggtgg cattccgcgt caggtttctg tgccgctggc
    36001 tgccgtgctg gctatctacg cccgtgaaaa tggcgcaggc acgatgtttg agcctgaagc
    36061 tgcctacgat gaagatacca gcatcatgaa tgatgaagag gcatcggcag acaacgaaac
    36121 cgttatgtcg gttattgatg gcgacaagcc agatcacgat gatgacactc atcctgacga
    36181 tgaacctccg cagccaccac gcggtggtcg accggcatta cgcgttgtga agtaatacaa
    36241 aacaggccca ggcggcctgt tttgtctttt taatgactta ataaaatagc gtgattgtgg
    36301 caatcatatt caccacgaaa ccgactgcca tggggatcgc tgtccgcttc accaccgcaa
    36361 aaggcgaaac gcctgcaatt cctgacgtaa cgaccaccac cgcagtaatc ggcgaaaccg
    36421 cgcgcgctag cgtcgtggca aaatgcatcg gcataatcat tacaaccgct ggtacatgta
    36481 gtccggctgc gatattcgga ataagactgg caaatgacat aaacggcgca ttgccagagc
    36541 ccatcacaat ggcacaaatg gcaatgacta gcgccataat aatcatcacg ccaataccgc
    36601 ccagaccaga atgctccgca cccctgataa ccgcatcaac agtgccaatc gtcgttaagc
    36661 ctttcgcaaa tatttcaccc gcgaccacca gcgttaccac gttgacaaac tgcgtaccca
    36721 tgccgtcaaa aaacgcctgc acatcgtcca ttgtttcgcg caagttatgc ttgcggaaga
    36781 actcaacaaa catcgtcacc gtcaaactta gtaacatcac caccaccaga tgcagttctg
    36841 acttcatcag cccgacgtgg gcgaggaaca gcgagccgag catcaggatt aacggcatca
    36901 caggtaaaat ggcgtaatag agcggcggga cattatcgag agttttttgc tctgcctgtt
    36961 cgtgattgat atttttatct tttttgtcaa aagcgcgttg cacgaaaaag tgggagatcg
    37021 ccaccgagat aatgacgcaa gaggcgaccg gaagctggta gtggaagaag tatgtggcaa
    37081 ttttcattcc cgcgacctgg gctgcaaaaa tggagttcgt ttccagaatc ccccactcaa
    37141 tggacatcgt ggttgcgata accgccactg cagagaggcg acttactccc agactcacca
    37201 gcgtcggaaa taaggtgacc atcagcaaca tacccagacc ggaggcgctg gtaataaact
    37261 gcgccatgat ttggccgatg acgtaagttg ccgacagaat aatatacggc gagcgaatga
    37321 gttttaacgg gcggcttagc aagctcacca tcgcgcgact ggctcctatg cgctccatgt
    37381 agcgggcata accgcccacc gccataatcg acagccccaa tccggcaatg cggttggaca
    37441 gcatgcgcag gatttcatta taaatgtcga cgatcaagta gccgctgctt ttggcgggat
    37501 cgagtacacc accgaaccct aaccagactc cgcacatcat caggaagaca cctgcggcag
    37561 ccagcaccac ctgaggtttg tagtttttca ggatcaaata gcccatcgta attaatacga
    37621 tgacagatat aattatgccg aacatt
//
LOCUS       DAJVQH010000021        36607 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJVQH010000021 DAJVQH010000000
VERSION     DAJVQH010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.021"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            27..2294
                     /gene="adiA"
                     /locus_tag="OQM87_001217"
     CDS             27..2294
                     /gene="adiA"
                     /locus_tag="OQM87_001217"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCS1507428.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            2619..3380
                     /gene="adiY"
                     /locus_tag="OQM87_001218"
     CDS             2619..3380
                     /gene="adiY"
                     /locus_tag="OQM87_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCS1507429.1"
                     /translation="MRICSDQPCIVLLTEKDVRIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            3517..4854
                     /gene="adiC"
                     /locus_tag="OQM87_001219"
     CDS             3517..4854
                     /gene="adiC"
                     /locus_tag="OQM87_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCS1507430.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            4958..6601
                     /gene="eptA"
                     /locus_tag="OQM87_001220"
     CDS             4958..6601
                     /gene="eptA"
                     /locus_tag="OQM87_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCS1507431.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            6598..7266
                     /gene="basR"
                     /locus_tag="OQM87_001221"
     CDS             6598..7266
                     /gene="basR"
                     /locus_tag="OQM87_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCS1507432.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            7267..8367
                     /gene="pmrB"
                     /locus_tag="OQM87_001222"
     CDS             7267..8367
                     /gene="pmrB"
                     /locus_tag="OQM87_001222"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCS1507433.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="OQM87_001223"
     CDS             complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="OQM87_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCS1507434.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8544..10046)
                     /gene="proP"
                     /locus_tag="OQM87_001224"
     CDS             complement(8544..10046)
                     /gene="proP"
                     /locus_tag="OQM87_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCS1507435.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(10310..11188)
                     /locus_tag="OQM87_001225"
     CDS             complement(10310..11188)
                     /locus_tag="OQM87_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCS1507436.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNSGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="OQM87_001226"
     CDS             complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="OQM87_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCS1507437.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            13815..14150
                     /locus_tag="OQM87_001227"
     CDS             13815..14150
                     /locus_tag="OQM87_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCS1507438.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            14308..14751
                     /gene="yjdN"
                     /locus_tag="OQM87_001228"
     CDS             14308..14751
                     /gene="yjdN"
                     /locus_tag="OQM87_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCS1507439.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            14884..15672
                     /gene="phnC"
                     /locus_tag="OQM87_001229"
     CDS             14884..15672
                     /gene="phnC"
                     /locus_tag="OQM87_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCS1507440.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            15697..16713
                     /gene="phnD"
                     /locus_tag="OQM87_001230"
     CDS             15697..16713
                     /gene="phnD"
                     /locus_tag="OQM87_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1507441.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSSGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            16819..17598
                     /gene="phnE"
                     /locus_tag="OQM87_001231"
     CDS             16819..17598
                     /gene="phnE"
                     /locus_tag="OQM87_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCS1507442.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            17619..18344
                     /gene="phnF"
                     /locus_tag="OQM87_001232"
     CDS             17619..18344
                     /gene="phnF"
                     /locus_tag="OQM87_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCS1507443.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            18345..18797
                     /gene="phnG"
                     /locus_tag="OQM87_001233"
     CDS             18345..18797
                     /gene="phnG"
                     /locus_tag="OQM87_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCS1507444.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMIRGDNA"
     gene            18794..19378
                     /gene="phnH"
                     /locus_tag="OQM87_001234"
     CDS             18794..19378
                     /gene="phnH"
                     /locus_tag="OQM87_001234"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCS1507445.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            19378..20442
                     /gene="phnI"
                     /locus_tag="OQM87_001235"
     CDS             19378..20442
                     /gene="phnI"
                     /locus_tag="OQM87_001235"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCS1507446.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            20435..21280
                     /gene="phnJ"
                     /locus_tag="OQM87_001236"
     CDS             20435..21280
                     /gene="phnJ"
                     /locus_tag="OQM87_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCS1507447.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            21277..22035
                     /gene="phnK"
                     /locus_tag="OQM87_001237"
     CDS             21277..22035
                     /gene="phnK"
                     /locus_tag="OQM87_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCS1507448.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            22146..22826
                     /gene="phnL"
                     /locus_tag="OQM87_001238"
     CDS             22146..22826
                     /gene="phnL"
                     /locus_tag="OQM87_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCS1507449.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            22823..23959
                     /gene="phnM"
                     /locus_tag="OQM87_001239"
     CDS             22823..23959
                     /gene="phnM"
                     /locus_tag="OQM87_001239"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCS1507450.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAQLGLLDILSSDYYPTSLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            23959..24516
                     /gene="phnN"
                     /locus_tag="OQM87_001240"
     CDS             23959..24516
                     /gene="phnN"
                     /locus_tag="OQM87_001240"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCS1507451.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLLVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            24503..24937
                     /gene="phnO"
                     /locus_tag="OQM87_001241"
     CDS             24503..24937
                     /gene="phnO"
                     /locus_tag="OQM87_001241"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCS1507452.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            24939..25697
                     /gene="phnP"
                     /locus_tag="OQM87_001242"
     CDS             24939..25697
                     /gene="phnP"
                     /locus_tag="OQM87_001242"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCS1507453.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRAQRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHAQGLFPLRWGVGDPIPIYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLIENALPSGFEAGFDGMEIGVA"
     gene            complement(25844..26173)
                     /locus_tag="OQM87_001243"
     CDS             complement(25844..26173)
                     /locus_tag="OQM87_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507454.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(26242..26874)
                     /locus_tag="OQM87_001244"
     CDS             complement(26242..26874)
                     /locus_tag="OQM87_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1507455.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRAAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGCCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(26955..29170)
                     /locus_tag="OQM87_001245"
                     /pseudo
     CDS             complement(26955..29170)
                     /locus_tag="OQM87_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            29284..30804
                     /locus_tag="OQM87_001246"
     CDS             29284..30804
                     /locus_tag="OQM87_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1507456.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            30801..31775
                     /locus_tag="OQM87_001247"
     CDS             30801..31775
                     /locus_tag="OQM87_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCS1507457.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            31806..32747
                     /locus_tag="OQM87_001248"
     CDS             31806..32747
                     /locus_tag="OQM87_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1507458.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            32802..33485
                     /locus_tag="OQM87_001249"
     CDS             32802..33485
                     /locus_tag="OQM87_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HCS1507459.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQRLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            33500..34360
                     /locus_tag="OQM87_001250"
     CDS             33500..34360
                     /locus_tag="OQM87_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HCS1507460.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            34357..35358
                     /locus_tag="OQM87_001251"
     CDS             34357..35358
                     /locus_tag="OQM87_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCS1507461.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(35355..36061)
                     /locus_tag="OQM87_001252"
                     /pseudo
     CDS             complement(35355..36061)
                     /locus_tag="OQM87_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            36352..>36607
                     /locus_tag="OQM87_001253"
     CDS             36352..>36607
                     /locus_tag="OQM87_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507462.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYY"
BASE COUNT         8529 a        10227 c         9717 g         8134 t
ORIGIN      
        1 attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc gagtttctcc
       61 atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta agccagcaaa
      121 atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc tcttcaaacg
      181 aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa catcaaaacg
      241 tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc ttcctgttgg
      301 gcgatcggga aaaagccctc gccgcaatgg accgggatct gctggagctt gtcgatgaat
      361 tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgccgtt gccgcgatga
      421 cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa tatagtgaca
      481 tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt accaaaacac
      541 ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc gacatgggca
      601 tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc gaaagcgaaa
      661 aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc ggtacttccg
      721 gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg gtcgttgacc
      781 gtaactgcca taaatccatc gaacaaggtt tgatgctgac aggcgcgaaa ccggtctata
      841 tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag gaaatgcaac
      901 ctgaaacctt gcaaaagaaa atcagtgaaa gcccgctgac caaagacaaa gccgggcaaa
      961 aaccgtctta ctgcgtggta accaactgca cctatgacgg cgtgtgttat aacgctaaag
     1021 aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa gcctggtacg
     1081 gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc gaacctggcg
     1141 atcacaacgg tcctaccgtt ttcgccaccc actccatcca caaactgctg aatgcgctgt
     1201 cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc tcccgcttca
     1261 accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc gcatccaacg
     1321 acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacacag gaagtgatta
     1381 acgaagcggt tgatttccgt caggcgatgg cgcggctata taaagagttc accgctgacg
     1441 gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa accggcaaaa
     1501 cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac tgctgggtaa
     1561 tacatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg agtatgctcg
     1621 acccaattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa ctggaagaaa
     1681 ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt gtgcctaccc
     1741 gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt gggaaatggg
     1801 ggactctggt taacaccctt tgctccttca aacgccacta tgacgccaac acaccgctgg
     1861 cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac atggggattc
     1921 acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc gcacggttga
     1981 acgaagccta ttccggcctg ccggtggcgg aaatcacccc gcgtgaagcg tacaacgcga
     2041 ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc atcgcggcaa
     2101 actcagttat cccgtatccg ccaggaatcc cgatgctgct gtctggtgaa aacttcggcg
     2161 ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac caccatttcc
     2221 ctggatttga acacgaaact gaagggactg aaattattga cggtatttac cacgttatgt
     2281 gcgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac attacgccgc
     2341 ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca aacaaaaatg
     2401 tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta tcctgtaaaa
     2461 aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact cgttattttt
     2521 atgcatgttt atattcatca tataattata taaccatttc ccggtatcgc tttgctttag
     2581 cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc agcgaccaac
     2641 cttgtattgt tttattgact gaaaaagatg tccggataag ggtgaatggg aaagaaccta
     2701 ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt atcgacgtct
     2761 cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa gactacctcc
     2821 ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct acgcccatac
     2881 tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac agcatgatgc
     2941 ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg gtgctatccc
     3001 gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt aattgtgtaa
     3061 gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat cttagtatgg
     3121 tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagttgaaa agcgaaaaca
     3181 ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat gaattaatga
     3241 tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt acgtcgtact
     3301 ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt agtcagcaca
     3361 gagaacgcac tgtcgcctga tttttaacct taacgaagag ctatattaat aacggcatca
     3421 gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata tgacgtaatt
     3481 tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc tgatgctcac
     3541 aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg gtcaggtgtt
     3601 tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg atggttggtg
     3661 acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt cctcgaccca
     3721 agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct cggttatcaa
     3781 accaacgtcc tctactggct ggcctgctgg atcggcaata tcgccatggt ggtcattggc
     3841 gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt aaccatcacc
     3901 tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa aatgatcacc
     3961 cgcgtgcagg ccgttgccac tgtgctggcg ctgatcccca tcgtcgggat tgccgtattt
     4021 ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag cggcctgggc
     4081 accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat cggtgtggaa
     4141 agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc tatcgccacc
     4201 attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc gattatgggg
     4261 atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc cgcacggatg
     4321 gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg ttgcttaggt
     4381 tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc cgatgacgga
     4441 ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc ggggctgatt
     4501 atcgtcggta ttttgatgac catcttccag ctcagcagca tttcaccaaa cgcgaccaaa
     4561 gagttcggtc tggtttcttc cgtctcggtc atctttacac tggtgccata tctttacacc
     4621 tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc ggcatatctg
     4681 gctgttacta ccattgcctt cctctactgc atttgggccg tggtggggtc cggggcgaaa
     4741 gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc gctgaattac
     4801 aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga ttaattcccc
     4861 ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa ttttgctttg
     4921 cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc tactaaaaag
     4981 accctctttg aatttactcg cctggctatt gttggccgct ttttatatct ctatctgcct
     5041 gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc tgcataacgt
     5101 actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg tcctgacact
     5161 aagctctttc ttatggctta atcgaacact ggcctgcctg tttattctgg ttggcgcggc
     5221 tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga ttgccaatat
     5281 tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt tattaacgct
     5341 gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca aacctgccac
     5401 ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg tactgctgat
     5461 tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca ataacaaaga
     5521 gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat ggtactccca
     5581 tcagcgactg gcaaatctgc cgctggtgcg aattggtgaa gacgcgcacc gcaacccgtt
     5641 aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa cctcgcgggc
     5701 ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg cgaaagataa
     5761 cgtggtctat ttccctaata ccgcatcttg cggtacggca acggcagttt cagtaccgtg
     5821 catgttctcg gatatgccgc gtgagcacta caaagaagag ctggcacagc accaggaagg
     5881 cgtgctggat atcattcagc gagcgggcat caacgtgctg tggaatgaca acgatggcgg
     5941 ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga atctgcctga
     6001 tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg aagagtacat
     6061 caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc acggtccgac
     6121 ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg acaccaatga
     6181 gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc tggtttacgt
     6241 cgactatatt gttgataaag cgattaatct gcttaaagaa catcaggata aatttaccac
     6301 cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca tctatctgca
     6361 cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccaatgc tgctgtggct
     6421 gtcggaggat tatcaaaagc ggtatcaggt tgaccagaac tgcctgcaaa aacaggcgca
     6481 aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa ctggcgttga
     6541 gacgaagtat taccaggccg cggatgatat tctgcaaact tgcaggagag tgagtaaatg
     6601 aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct ggcggcgcaa
     6661 accgaaggct acgcgtgcga tggcgtgaca accgcgcgga tggcggaaca aagccttgag
     6721 gacggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga tggactgcat
     6781 tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct caccgctcgc
     6841 gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta tctggtgaag
     6901 ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg ccataataat
     6961 cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg ccgtcaggta
     7021 tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct gtcacggtta
     7081 atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat ctataactgg
     7141 gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg cgacaaagtg
     7201 ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc gaatgaggaa
     7261 aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca acggctgata
     7321 ttgaccatcg gggccatttt gttggtgttt gagctgatca gcgtcttctg gctatggcat
     7381 gaaagtaccg agcagattca actgtttgag caggcgctac gcgacaatcg caacaacgat
     7441 cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc cggcgtcttt
     7501 atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac ccgcccgctg
     7561 gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc cattgccatt
     7621 cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct ggtcagtcgc
     7681 ctgaccagca cgctggataa cgaaaggttg tttaccgctg acgtcgcgca cgaactgcga
     7741 acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca tcacattgat
     7801 gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca gctgctgcaa
     7861 ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa actgctggaa
     7921 gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg acagcaaacc
     7981 ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac cctgctgcgg
     8041 atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccgcaagg cagcaacatt
     8101 atgattaagc tgcaagaaga tggcggagcg gtcatggcag ttgaagatga aggaccaggt
     8161 attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga cagccgttat
     8221 ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca tcacgggcag
     8281 tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg gctgaagaaa
     8341 gatcagaacg tggcaaacca gatataaaga aagctgctga ccaccagcac gctgaacacg
     8401 gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa cctggaggag
     8461 agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat aagacagcgt
     8521 cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg caccaggcgg
     8581 gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat tatcgatttt ctgctcgata
     8641 ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc cgccggtgtc
     8701 gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat taaaccaatc
     8761 accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct ttcgaccagc
     8821 caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa tgccgccgcc
     8881 agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc cataacgccc
     8941 ataaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag gccgatgacg
     9001 ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagtgcaac actaccaagt
     9061 agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg ctggacaaac
     9121 agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga gtaatgcagg
     9181 ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac gttggtggca
     9241 attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat ctctttaaac
     9301 gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt atcaacatgc
     9361 tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat aatccctaac
     9421 ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc ttcgccgaca
     9481 atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat agaaccgaag
     9541 tccagccagc tgcccataaa gccacgttta cggtcggggg agtattccgc aacaaatatc
     9601 gacgccccgg tatattcacc accgaccgag aaaccttgtg ccatcttaca gatcaacagc
     9661 agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat acagaacgta
     9721 ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata tttatcgccc
     9781 aacataccaa agaagagtcc gccaagcggt cgaatcagaa agggaacgga gaaagtggca
     9841 agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt acctaatgcg
     9901 taagcaacaa aaccataaac accaaaatca aaccattcca ttgcattacc cagtgatgct
     9961 gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg aagggtaatc
    10021 ggttttactt ttttcctttt cagcatagct ttcctcgcag agttgaccca tttccgcaaa
    10081 cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg atgcaatctc
    10141 tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca atcaaaccag
    10201 ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta aatttgtgat
    10261 caacttcaca catggatttc gtttaggact cattgatgta actgattatt tacgccaaat
    10321 ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa cagagtcgtg
    10381 gcatccccag cgcgacagtt tgccagcgtt gggtcactga ccagcaaacg ggttgtttat
    10441 ctgccgtggt ccactttccc agccaactta aaaaatcagc atgatccccg cccgtcggaa
    10501 cggtcgaaga ggcaaaccac tgatggtaat agtgacgcgt ggtcggtgcc gcattgatac
    10561 tctctgccag atgttgtgtc gctttctcgc gcaaatcacc cagcaacaac accaccgctt
    10621 gcgggcttcc cagacggcag gcatcgccaa atgcacccca gcgcaactcc tccagaacga
    10681 taaaacagcg cccaggcaac gaccaggcgt tatacgcccc agcacgccag cgggtaaaaa
    10741 tttgctcgct ctgatcacct gcctgcacca gtaggcctcg ttgcgttaac gccttttcct
    10801 tccagcgaca acgggctgaa aaccactcat acaaggcggc aatttgctgg gggagctccg
    10861 ggaaagcaga gtgctggcgg tgatccattt tttgcagcag cgccagatgc tgcgcgatct
    10921 gcgtcagcgc cagatgaccg ggatccagca tcgacgccag tttttccacc attgtcagtc
    10981 gcagactaag cggcgcattt aacatgccgc tgttcgtcca tgtctgaagc tgcacacgcg
    11041 aaagaagttc ctgcatcagt cgttcacgaa actgctgctg atgggcttga ggatggcggg
    11101 catcatcgcc ctgcgccaga tccactaaaa agcgcggatg aaccgactcc agcacgcgac
    11161 cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc
    11221 atcgcgtaac agctgagcgt cggtgtaaat ccagctgtga gtcatcacag tctgctgcaa
    11281 ttgctgctgc atcagcctga ccaccgattc attttgttga cgcagagcca gactttcgcg
    11341 taaacgcgtc tgtaattctg tcaaacatga agcgaactca gcgaaaaaag tattcatgcc
    11401 tgccgtaaca gattcatcga cctgctctgc cagaacttta gccatttgtt ggcaatagag
    11461 atcgacttct gcgcttaatg ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga
    11521 tttctcattt ccccgtcccc agtcgggctg attcaaccag cgcgaaaaag tctcacgcac
    11581 aacgcctaaa cgcgtgctct gctcgtccgt tgcttcctgg cgcgaaatga cttcactgaa
    11641 cagctggcga gtgttgaagt ggggaactac gccgtgaaaa acaggaaaat gaaacccagg
    11701 acgaaaccct gactcgctca attccatttt gacttgttgc tcaatggggc gaataacatc
    11761 ggttaacacc cggcaaaggg tggattccag ctcggcaaaa cgcagcgtaa agtcgcgact
    11821 gatggtgttc tgcgccgtct gtaacagtgt ctcacagcgg gtacgaatct cgcttaacgg
    11881 ctccgaatca tcctgaaaca aggcggctaa ctgcgcattc accgcatctt gctgttgacg
    11941 cagaaagtgg ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac
    12001 ctgcgcctga ttgagttgca atagctgcaa actttcttcg acctgatgga tattttgccg
    12061 caattgttca caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg
    12121 ctgcgcgtaa ttcaacagtt tatgcgcagc agatcgcaga gcatacaatg aggcgttagc
    12181 gtaagcggca tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg
    12241 atcggcagca tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc
    12301 gagcgcggca tgggcaaaat cttccaccca gcgttgttgc tctggcgctg gtaacttacc
    12361 gttgttggct aactcatagc gcgcccgatt cgccaggtag ccccacatcg acgacaccgg
    12421 gaatatctgc tgtggcgtaa tacagccttt catcagcgtc ccggaaatca gtgcccgcac
    12481 ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacag
    12541 cggcaccgat tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt
    12601 cagttgcgta taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag
    12661 cattttttgc agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa
    12721 ctgaccggga taactttcca gccccgccag atggacaaac tccacttcaa tcacgggaat
    12781 atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg
    12841 cactaaatca ttcaaacttt tcagacaatg aaaaataggc tgggcaccca gataatattt
    12901 ttcgaaagcg acgccatttt cgatccgctg cataagcgca cgcatatctt tatctatttc
    12961 cagcacatcg gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat
    13021 taaacaatcg attggcgcga catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt
    13081 atggcgaata agcgtcggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt
    13141 accaacaatg gcattaatgg tggttgattt ccctgctttc atggtaccga caattgccag
    13201 caccatttcc agtcgggaaa ttttacgcaa ctcattattc agcatcgcgt gacgttcggc
    13261 gatattaggc tgactccagg gtaaagccag ttgtggcgcg tcgtctccgg gtacagagag
    13321 aggcattttt tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc
    13381 ctgacttaac tcatacaggg tctgtgtgta catagaaaat tcttccttaa agcaaatttt
    13441 gttactttta tttagccaga ttgtttttga gttctgtttt cggcttttat aattactgca
    13501 agaaataatt ttatatttag tgtgttgttt tttatcagaa taaataacgt cttctgatgc
    13561 gtttaaaacg tcagaaagat aaaaatatca tgtaaataaa aaaaagaaca agtagagcat
    13621 taacattatc ttaaataata aatagaggca aaaagattat tttctttttg cgtttccttt
    13681 caaatgaaaa cgatcgtcgt ctaaaatcag cagtaccccc gacaaactca gggattttgt
    13741 gtataattgc ggcctttttc ggcaatctgc cgttttttgg cgcttttgcc ctgctgactt
    13801 ttgaggaaat ccacatgtca ttaccacact gcccaaaatg caactccgaa tacacttacg
    13861 aagataacgg catgtacatc tgcccggaat gtgcctacga atggaacgac gcagaacctg
    13921 cacaggaaag cgacgagctg atcgttaaag atgctaacgg caatctgctg gctgacggcg
    13981 acagcgttac catcattaaa gatctgaagg tgaaaggtag ctcttcgatg ctgaaaattg
    14041 gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca taacatcgat tgcaaaatcg
    14101 acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa aaagaactga ttgtattgtg
    14161 atcggtaagc cgaataaggc gctcgcgccg catccggcaa cgatgccgac tgcctgatgc
    14221 gacgcttgcg cgtcttatca ggcagcctct catctgtata aatttcgaac tacacttaac
    14281 tggcttctct taactgaggt caccatcatg ccgttaagtc cctacctctc ttttgccggt
    14341 aactgttccg acgcgattgc ctattatcaa cgtacgttgg gcgcggaact gctctataaa
    14401 atcagcttcg gcgaaatgcc gaaatcagcg caggacagcg ccgagaactg cccttccgga
    14461 atgcaatttc ccgataccgc catcgctcat gccaacgtgc gcattgccgg aagcgacatc
    14521 atgatgagcg atgctatacc gtcaggaaaa gccagctact ccggctttac gctggtgctc
    14581 gattcgcaac aggtcgaaga aggaaaacgc tggtttgata accttgccgc taacggaaaa
    14641 atcgaaatgg cctggcagga aactttctgg gcgcatggct ttggcaaagt caccgataaa
    14701 tttggcgtac cgtggatgat taatgtcgtc aaacaacaac caacgcaata acccgccggg
    14761 aggcccgccc tcccgcactg tcatcgaatt cccgttaact cttcatctgt tagtcacttt
    14821 taattaacca aatcgtcaca ataatccgcc acgatggagc cactttttta gggaggctgc
    14881 atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa ccttcaatca gcatcaagcg
    14941 ctgcatgcgg ttgatctgaa cattcatcac agtgaaatgg tggctctgct tgggccgtcg
    15001 ggttccggca aatccaccct tttacgtcat ttaagcggtt tgattaccgg cgataaatcc
    15061 gccggtagcc atatcgagct actgggccgc actgtccagc gcgaaggccg cctggcccgc
    15121 gatatccgca aaagccgcgc caacaccggc tacatctttc aacaattcaa cctggtgaac
    15181 cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg gcagcacgcc gttctggcgc
    15241 acctgtttta gctggttcac cggcgagcag aaacagcgcg cgttacaggc gctgacccgc
    15301 gttggcatgg tgcattttgc ccatcagcgc gtttccaccc tctccggcgg ccagcagcaa
    15361 cgtgtggcga ttgcccgtgc gctgatgcag caggcgaaag tgattctggc cgatgaaccc
    15421 atcgcctcgc tggacccgga atcagcgcgc atcgtgatgg acaccctgcg cgacatcaac
    15481 cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg tggattacgc cctgcgctac
    15541 tgcgaacgca tcgtcgccct gcgtcagggg cacgtcttct acgacggcag cagccaactg
    15601 tttgataacg aacgttttga ccatctctac cgcagcatta accgcatcga agagaacgcg
    15661 aaagctgcct gatatcccca tcattgagga aaacgaatga acgctaagat aattgcctcg
    15721 ctggccttca ccagcatgtt cagcctcagc accctgttaa ccccggcaca cgccgaagag
    15781 caggaaaagg cgctgaattt cggcattatt tcaacggaat cacagcaaaa cctgaaaccg
    15841 caatggacgc cgtttttaca ggatatggag aagaaactgg gcgtgaaggt caacgccttc
    15901 tttgccccgg actacgcggg cattatccag gggatgcgct tcaataaagt ggatattgcc
    15961 tggtacggca acctgtcggc gatggaagcg gtggatcgcg ccaatggcca ggtcttcgcc
    16021 cagacggtcg cggcggatgg atcgtcgggt tactggagcg tgttgatcgt caacaaagac
    16081 agcccgatca acaacctgaa cgatctgctg gcgaagcgga aagatctcac cttcggcaat
    16141 ggcgatccta actccacctc tggcttcctc gtccccggtt actacgtctt cgctaaaaac
    16201 aatatctccg ccagcgactt caagcgcacc gtcaacgccg ggcatgaaac caacgcgctg
    16261 gccgtcgcca acaagcaggt ggatgttgcc accaacaaca ccgaaaacct cgacaagctg
    16321 aaaacctccg cgccggagaa gcttaaagag ctgaaggtga tctggaaatc gccgctgatc
    16381 ctaggcgatc cgatcgtctg gcgcaagaat ctgtccgaaa ctaccaaaga caagatctac
    16441 gacttcttta tgaactacgg aaaaacgccg gaagagaaag cggtgctgga acgcctaggc
    16501 tgggcgccgt tccgcgcttc cagcgacctg caactggtgc cgattcgcca gctcgcactg
    16561 tttaaagaga tacaaagcgt gaaaggcaat aaagggctga atgagcagga caagctggcg
    16621 aaaaccaccg agattcaggc gcagctggat gacctggacc gcctgaacaa cgcgttaagc
    16681 gcgatgagtt cggtgagtaa agcggtgcag taaatcgtag gtcggataag acgccccggc
    16741 gtcgcatccg acaatgtgca ggcgttgatg ccggatgcgg tgcaagcacc ttatccggcc
    16801 tacagaccgg agccaaacat gcaaaccatc accatcgccc cacccaagcg tagctggttc
    16861 tcgcttctga gctgggccgt tgttctcgcc gtgctggtcg tctcgtggca gggcgcggaa
    16921 atggccccgc ttacgctgat taaagacggc ggcaacatgg caaccttcgc tgccgacttc
    16981 ttcccacccg atttcagcca gtggcaggat tacctcaccg aaatggccgt cacgctgcaa
    17041 atcgccgtct ggggcaccgc gctggcagtg gttctctcca tccccttcgg tctgatgagc
    17101 gccgaaaact tggtgccgtg gtgggtttac cagcccgttc gccgcctgat ggacgcctgc
    17161 cgcgccatta acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg cctcggcccg
    17221 ttcgctggcg tgctggcgct gtttatccac accaccggcg tgctctccaa gctgctttcc
    17281 gaagcggagg aagcgatcga acccggcccg gtggaaggca ttcgcgccac cggcgccaac
    17341 aaactcgaag agatccttta cggcgtgctg ccgcaggtga tgccgctgct gatctcctac
    17401 tccctctatc gcttcgaatc caacgtccgc tcggcgaccg tcgtcggcat ggtcggcgcg
    17461 ggcgggatcg gcgtcaccct gtgggaagcg attcgcggtt tccagttcca gcaaacctgc
    17521 gccctgatgg tgcttatcat cgtcacggtc agcctgctgg atttcctctc tcaacggttg
    17581 cgtaagcact ttatctgata agcgaggcat tgatatctat gcacttgtct acacatccga
    17641 ccagctaccc aacacgctat caggagatag ccgcaaaact tgagcaggag cttcgtcaac
    17701 actaccgctg cggcgactat cttcccgccg agcagcaact ggcggcgcgc tttgaggtga
    17761 accgccacac cctgcgccgc gccatcgacc aactggtgga aaaaggctgg gtacagcgcc
    17821 gtcagggcgt cggcgtgctg gtgctgatgc gcccgttcga ttacccgctc aacgcccagg
    17881 cgcgttttag ccagaacctg ctggaccagg gcagccatcc caccagcgaa aaactgctgt
    17941 cggtattgcg ccccgcgtcc ggtcacgttg ctgacgcgct ggggattacc gagggcgaga
    18001 acgttatcca tttgcgcacc ctgcgccggg tcaacggcgt cgcgctctgt ttaatcgacc
    18061 actactttgc ggacctcacc ctttggccga cgctgcaacg cttcgacagc ggttcgctgc
    18121 acgattttct gcgcgagcaa accggaattg cgctgcgccg cagccagacg cggatcagcg
    18181 cccgccgcgc ccaggccaaa gagtgccagc gccttgaaat cccgaatatg tcgccgctgc
    18241 tgtgcgtgcg cacccttaac caccgtgacg gtgaaagcag cccggcggag tactccgtca
    18301 gcctgacgcg cgccgacatg attgaattca ctatggagca ctgaatgcac gcagataccg
    18361 cgacccgcca gcactggatg tccgtactgg cgcacagcca accggctgaa ctggcagccc
    18421 gcctgaacgc gctgaacatc accgccgact atgaagtgat ccgcaccgcc gaaaccggcc
    18481 tggtacagat tcaggcgcga atgggcggca ccggcgaacg cttttttgcc ggcgacgcca
    18541 cgctgacccg cgccgccgta cgcctgactg acggcaccct cggctacagc tgggtgctgg
    18601 ggcgtgataa acagcacacc gaacgctgcg cgctgattga tgcgctgatg cagcaatctc
    18661 gccactttca aaacttatca gaaaccctta ttgccccgct ggacgctgac cgtatggcac
    18721 gcattgccgc acgccaggcc gaagtgaacg ccagccgggt cgacttcttt acgatgattc
    18781 gcggagacaa cgcatgaccc tggaaaccgc ttttatgctt cccgtgcagg atgcccagca
    18841 cagttttcgt cgcctgttaa aggccataag cgagccgggc gtgattgtcg ccctgcatca
    18901 gctcaagcgc ggctggcagc cgctgaatat cgccaccacc agcgtgctgc tgacgctggc
    18961 tgataacgac acgccggtgt ggcttgccgc gccattgagt aacgatatcg tcaatcagag
    19021 cctgcgtttt cataccaacg cgccgctggt cagccagccg gaacaggcga ccttcgcggt
    19081 gacggatgac ggaatttcca gcaaacagct caacgccctt tccaccggca ccgccgttgc
    19141 gccggaagcg ggcgcgacgc tgattttaca ggtcgccagc ctgagcggcg ggcgcatgtt
    19201 gcgtctcacc ggcgcgggta ttgccgaaga acgaatgatc gctccgcagc tgccggagtg
    19261 cattctgcac gaactcaccg agcgcccgca cccgttcccg ctcggcatcg acctgatcct
    19321 gacctgcggc gaacgcctgc tggctattcc gcgaaccact catgtggagg tgtgctgatg
    19381 tacgttgccg tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct gcaagagagc
    19441 cgacgccggg gcgataccga tttgcccgaa ctgagcgtcg cccaaattga acagcagctt
    19501 aatctcgcgg tggatcgcgt gatgaccgaa ggcggcgttg ccgaccgcga actggcggcg
    19561 ctggcgctga aacaggccag cggcgataac gttgaagcaa ttttcctgct gcgcgcctac
    19621 cgcaccacgc tggcgaaact ggcggtaagc gagccgctcg acaccaccga gatgcgtctc
    19681 gaacggcgta tttcggcggt ttataaagac attcccggcg gccagctgct tggcccaacc
    19741 tacgactaca cccatcgcct gctcgatttc accctgctgg caaacggtga agcgccgacg
    19801 ctgaccaccg ccgacagcga acaacagccg tcgccgcacg ttttcagcct gctggcgcgt
    19861 caggggctgg cgaagtttga agaagatagc ggcgcacagc cggatgacat cacccgcacg
    19921 ccgccggttt acccctgctc acgctcctcc cgcctgcaac aattaatgcg cggggacgaa
    19981 ggctatttgc tggcgctggc ctactccacc cagcgtggtt acggacgcaa tcacccgttc
    20041 gcgggcgaga tccgcagtgg ttacatcgac gtgtcgattg tgccggaaga gctgggattt
    20101 gcggtaaacg tcggcgaact gctgatgacc gagtgtgaaa tggtcaacgg ttttatcgac
    20161 ccgccggatg agccgccgca cttcacgcgc ggctacgggc tggtattcgg catgagcgag
    20221 cgcaaagcga tggcaatggc gctggttgac cgtgcgttgc aagccccgga atacggcgag
    20281 cacgcgacag gcccggcgca ggatgaagag ttcgtgctgg cgcatgccga caacgtcgaa
    20341 gccgcaggct ttgtctcgca cctcaaactc ccccactacg tcgatttcca agccgaactg
    20401 gagctactca aacgtctgca acaggagcag aaccatggct aatctgagcg gctacaactt
    20461 tgcctacctc gacgagcaga ccaaacgcat gatccgccgc gccatcttaa aagcggtggc
    20521 tatccccggt tatcaggtgc cgtttggcgg gcgcgagatg ccgatgccct acggctgggg
    20581 aaccggcggc attcagctca ccgccagcgt gattggcgaa agcgacgtgt tgaaggtgat
    20641 tgaccagggc gcagacgaca ccactaacgc cgtgtcgatt cgcaacttct tcaagcgcgt
    20701 gaccggggtg aacaccactg aacgtacgga cgatgcgacg cttatccaga cgcgccatcg
    20761 catccccgaa acgccgctga ccgaagatca gatagttatt ttccaggtgc cgatccccga
    20821 accgctgcgc tttatcgagc cgcgcgaaac ggaaacccgc actatgcacg cgctggagga
    20881 gtacggcgtg atgcaggtga aactgtatga agatatcgcc cgcttcggtc atatcgccac
    20941 cacctatgct tatccggtga aggtgaacgg gcgctacgtg atggacccgt cgccgatccc
    21001 gaaattcgat aacccgaaaa tggacatgat gcccgccctg cagctgttcg gcgcagggcg
    21061 cgagaagcgc atctatgcgg tgccgccgtt tacccgcgtg gaaagtctcg atttcgacga
    21121 tcacccgttc accgttcagc agtgggatga gccatgcgcc atctgcggat cgacccacag
    21181 ctatctcgac gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct gctccgatac
    21241 cgattattgc cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt actttccgtc
    21301 aataacctga cccaccttta cgcgccgggc aaaggcttta gcgatgtctc ttttgattta
    21361 tggccggggg aagtgctggg catcgtcggg gaatccggct ccgggaagac cacgctgctg
    21421 aaatcaatct ccgcgcgcct gacgccgcag cagggggaga ttcgctacga gaatcgttcg
    21481 ttgtatgcga tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg gggcgtggtg
    21541 catcagcatc cactcgacgg cctgcgtcgc caggtctcgg caggcggcaa tatcggcgag
    21601 cggctgatgg cgaccggggc ccgtcattac ggcgacatcc gcgccaccgc gcagaagtgg
    21661 ctggaagagg tggagattcc cgccaaccgg atcgacgacc tgccgaccac cttttccggc
    21721 ggcatgcagc aacgtttgca gattgcccgc aacctggtga cgcatccgaa gctggtgttt
    21781 atggacgagc caaccggtgg gctggatgtg tcggtgcagg cacgcctgct cgatctactg
    21841 cgcggcctgg tggtggagct gaacctggcg gtggtgattg tcacccatga tttaggcgtc
    21901 gcccgcctgc tagcggaccg tttgctggtg atgaagcagg ggcaagtagt ggagagtggg
    21961 ttaaccgacc gcgtgctcga cgacccgcac catccgtata cccagctgct ggtgtcatcg
    22021 gttttgcaga attgagccgg tgccggatgc ggcgtaaacg ccttatctgg cctacaaatg
    22081 cgctccccgt aggtcggata agacgcatca gcgtcgcatc cgacacccga accacgaggc
    22141 gaaaaatgat taacgtacaa aacgtcagta aaaccttcat cctgcaccag caaaacggcg
    22201 tgcgcctgcc cgtcctcaac cgcgcctcgc tcaccgtcaa cgcgggcgaa tgcgtggtgc
    22261 tccacggcca ctccggcagc ggcaaatcaa ccctgctgcg ctcgctgtac gccaactatc
    22321 tgcccgatga agggcaaatc cagatcaaac acggtgacga gtgggtagac ctggtcaccg
    22381 cgccagcgcg caaagtggtg gagatccgca aaaccaccgt cggctgggtg agccagtttc
    22441 tgcgcgtcat cccgcgtatt tcagcactgg aagtggtgat gcagccgctg ctcgataccg
    22501 gcgtaccgcg tgaagcctgc gccgctaaag ccgcgcgtct gctcacccgc ctgaacgtgc
    22561 cggaacgcct gtggcacctg gcgccatcaa ccttctccgg cggcgaacag cagcgcgtca
    22621 acatcgcccg cggctttatc gtcgactacc cgattctgct gctcgacgaa cctaccgcct
    22681 cgctggacgc caaaaacagc gccgcggtgg tggaactgat ccgcgaagcc aaagctcgtg
    22741 gcgcagccat tgtcggcatc ttccatgacg aagccgtacg taatgacgtt gccgaccgcc
    22801 tgcacccaat ggaagcctct tcatgattat caataacgtc aaattggtgc tggaaaacga
    22861 agtggtacac ggttcactgg aggtgcagga cggcgaaatc cgcgcctttg ccgaaagcca
    22921 gagccgcctg ccggaggcga tggacggcga aggcggctgg ctgctgccgg ggctgattga
    22981 gctgcatacc gataatctgg ataaattctt caccccgcgc ccgaaagtcg actggcccgc
    23041 ccattcggcg atgagcagcc acgacgcgct gatggtggca agcggcatca ccaccgtact
    23101 ggacgccgtg gcgattggtg atgtgcgcga cggcggcgat cggttggaga atctggagaa
    23161 gatgatcaac gccatcgaag agacgcagaa acgcggcgtc aaccgcgccg agcaccgtct
    23221 gctcctgcgc tgcgaactgc cgcatcacac cacgctgccg ctgtttgaaa aactggtgca
    23281 gcgcgagccg gtgacgctgg tgtcgctgat ggaccactcg ccgggccagc gccagttcgc
    23341 caaccgcgag aagtatcgcg aatattatca gggcaaatac tcgctcaccg atgcgcagat
    23401 gcagcagtac gaagaagagc aactggcgct tgccgcacgc tggtcgcagc cgaatcgcga
    23461 atccatcgcc gccctgtgcc gcgcgcgaca aattgcgctc gctagccatg atgacgccac
    23521 ccacgcccac gttgccgaat cccacctgct tggcagcgtg atcgccgaat ttcccaccac
    23581 gtttgaagcg gcggaagcct cgcgcaagca tggcatgaac gtactgatgg gcgcgccgaa
    23641 tatcgtgcgc agcggttcgc actccggcaa cgtggcggcc agtgaactgg cgcagcttgg
    23701 cctgctggat atcctctctt ccgactacta ccccaccagc ctgctggatg cggcatttcg
    23761 cgtcgccgat gaccagagca accgctttac gctgccgcag gcggtgaagt tagtgaccca
    23821 aaatccggcg caggcgctta atcttcagga tcgcggggtg attggcgagg gcaaacgcgc
    23881 ggacctggtg ctggcacatc gcaaggacaa tcatattcat atcgaccacg tctggcgtca
    23941 gggtaaaagg gtgttctgat gatgggaaaa ctgatttggt taatggggcc gtccggctcc
    24001 gggaaagaca gcctgctggc ggaaatccgc ctgcgggaac aaactcagtt actggtggcg
    24061 catcgctaca ttacgcgcga cgccagcgcc ggaagtgaaa accatatcgc cctgagcgag
    24121 caggagtttt ttacccgcgc ggggcaaaat ctgttggcct taagctggca cgctaacagt
    24181 ctgtattatg gcgtcggcgt tgagattgat ctctggctgc acgccggatt cgacgtgctg
    24241 gtcaacggct cacgcgccca tctgccgcag gcgctggcgc gctatcaatc ggcgctgctg
    24301 cccatttgct tactggtttc gccggagatc ctgcgccagc gcctcgaaaa ccgtggtcgt
    24361 gaaaatgcca gtgaaattaa cgcccgacta gcacgcgctg cccgctatac tcctcaggat
    24421 tgtcttacgc tcaataatga cggcagcctg cgccagtcgg tcgacaagct gctgacgctg
    24481 attcatcaga aggagaaaca ccatgcctgc ttgtgagctt cgcccggcta cgcagtacga
    24541 caccgacgca gtttacgcgc tgatttgtga gctaaaacag gcggagtttg accaccatgc
    24601 gtttcgcgtg ggctttaacg ccaacctgcg cgacccaaac atgcgctacc atctggcgct
    24661 gctggatggc gaagttgtca gcatgatcgg cctgcatttg cagtttcatc tgcatcatgt
    24721 caactggatc ggcgaaattc aggaactggt ggtgatgccg caggcgcgcg gtctgaacgt
    24781 cggcagtaaa ttactggcat gggcagaaga agaagcccgc caggccgggg ccgaaatgac
    24841 cgcgctttcg accaacgtga aacgccacga cgcgcaccgt ttctatctgc gagaaggcta
    24901 cgagcagagt cacttccgct tcaccaaggt gctgtaacat gagcctgacc ctcacgctca
    24961 ccggcactgg cggcgcacag ggcgttccgg catggggctg cgagtgcgcg gcctgcgacc
    25021 gagcgcagcg ctcaccgcag tatcgccgcc agccatgcag cggcgtagtg aagtttaacg
    25081 acgcaatcac cctgatcgac gccgggctgc acgatctcgc cgatcgctgg tcgcccggat
    25141 cgttccagca gtttttgctg acgcattatc atatggatca cgcccagggg ctgtttccgc
    25201 tgcgctgggg cgtgggcgat ccgatcccga tctacggccc gccggatgac cagggttgcg
    25261 acgatctgtt taaacatccg ggcctgcttg atttcagcca cacagtggaa ccgtttgtgg
    25321 tgtttgattt gcaggggttg caggtcacgc cgctgccgct taaccactca aaactgacct
    25381 tcggttatct gctggaaacg gcacatagcc gggtggcgtg gctatctgac accgctggcc
    25441 tgccggaaaa aacgctgaaa tttttacgca ataatcagcc acaggtaatg gtgattgatt
    25501 gcagccaccc gccgcgcgcg gatgcgccgc gtaatcactg tgatttaaat accgtgcttg
    25561 cgctgaatca ggttatccgc tcgccacggg tgattctgac ccatatcagc caccagtttg
    25621 atgcgtggct gatagaaaac gcactgccgt cggggtttga ggcggggttt gatgggatgg
    25681 agattggggt ggcgtgatga gagggaatgt gcgcgctggt cccctcaccc taaccctctc
    25741 cccagagggg cgaggggagt gattgtgctc gatattgaat attgcgctgg ttttctccct
    25801 ctccccattg gggtgagggg cgatgcctgc tccataccca acctcatcgc ccatactcat
    25861 cttccattct ccgctcttca tcttctagct gccgacgctc ctgatcaagc tggcgctggc
    25921 gatcgtccag ctgcctgcgg cgatcttcaa actggcagcg gcggtcgtca tattgtctgc
    25981 gccgatcgtc gctcacttca cgctgccagc cgtcgtcgcg cgaatcttca tagtctcgcc
    26041 cacggtcaga gttataagcg tcattaatcg cctgctgaat attaccaata gtatcgtcga
    26101 taatatcggc ctgggccgga acggtggaca gcgtgagcag ggtgaaaaga aaaagcgtgg
    26161 agtaaggttt cataagccag tctctgcgat ggactggctt aacgttagtt gagtgtgaat
    26221 gaccggagta gcggagtaat ctcaaattcg gtaaggcgca gactcttcca ttagctggtt
    26281 agctcgctgt ttattttccg ccaccaatac aatgccatga catttttgat ccggtgaaat
    26341 actccagata cgctcttccg gaatgcagca gccatcgtag atcactttcg tgccatcgta
    26401 gggccaaaat gccccttgtt cagtgtctaa tacaaacacg gaaatctgat cggactcgcg
    26461 cgcgatcttc tgccagagca tcttcccgcc atcatattga tcaaaatcgc tgattagcgt
    26521 gataccgcat tgcaggatta gcgcttcata cagccgagtt gccagaccct tatcgcgctc
    26581 acctggagag acacaaatac cttccacctg taccagatcc gagtcttccc accagcgttc
    26641 aacggcaatg atatcgctgc cgaactcttt tttacgctgc agatcgagag cggcaagtac
    26701 ccgatgacgc ggcacgcctg caaaaacttc aatgtccgct tcaatgatgg ccgctcttgg
    26761 cgtctggttg tcataaatta cctgtaacag acgtccagaa agctcgatct cttccaccac
    26821 gcgaaaagaa cgagcaaccg tcatagggct ataggtggtt ggcatcacga tcatatgtaa
    26881 atcacttcaa ttcagaacag aattgctcac aatagcagca cgtttgcttc gagcctcttg
    26941 ataaattctg aaaatcatcc ctcaatttgc cccagcacct gcgccagctg cgcgcgggta
    27001 aagggtttgc gcagcagcgc cacgtccggc agcgcggggt tatggctggg gcgtaaatcc
    27061 tgaccgctga tgagtagcag cgtcaggtgc gggtaaagtt tgcgggccgc attgaccact
    27121 tctgcgccgc tcatgccgcc agcaggtcca gcgcctgttc gccgctgctc gcttccagtg
    27181 tcaaatagcc cagcaggtgc aactgctcgc agatggtctg gcgcaccgcg gcttcatctt
    27241 ccagcactaa caccagttta tcgccattaa cagctgtctg ttctaccgcc gctggcgata
    27301 ggtttttcgc cgccgtcagc gcgcggggaa gctgtaatct gaccgttgtc ccctgccccg
    27361 gcgcgctttc aatttccacg cgcccgccgg actggcgcac gaaaccatag accatcgaca
    27421 agcccagccc gctgccgctc cccgtctgtt tggtggtaaa gaacggctcg aacacctggc
    27481 ttttcaccgc ctgcgacata ccgctgccat gatcgatgac ctccagtgcc accatgtcct
    27541 gacgctgccc actgctgcgg gtaacacgct gattccaggt acgaattttg ataattcccg
    27601 tctgcccttc catcgcgtcg cgggcgttca tcaccaggtt gatgatggcg ttttccagtt
    27661 ggctgacgtc aatccacgcg gaccacgccg gagactgcgc ttcaatctca atcgtcagcg
    27721 tcgccggtaa ggagtggcgc attaattcgc ttaactcttc cagcagtgtt tttagttcaa
    27781 cggcttgcgg atgtaaggac tgcttgcggg aaaatgccaa caatcgctgg gtcagtagcg
    27841 cgccacgttc ggcggcttgc tgcgcccgct gtattcgcgg cgcgtcgggt gagttcgtgt
    27901 tcaccagttc caggctacca ataattaccg ccagcagatt attaaaatca tgggccagcc
    27961 cgcccgtcag ttgcctgacc gcttttaact tctggctgtg caatagcgcc tcttccagcc
    28021 cctgccgctc ggtttgggca atctccatct gcgtggtttt ctctttcaac agccgtgacg
    28081 ttcgttcaag tgatgcggta ttgcgggcaa aaacgttaaa ggcgcgcgcc agttcaccca
    28141 actcatcgcg ccgctgtaac gccggaaaag tgacgtccgc ttcgccgtgc gccaggcggc
    28201 tcatcgcgcg ggaaatcgcc gtcaggttag agccgagatt gcgatagata taccaacctg
    28261 caaacgcggt gatcaccacc gctaacaggg cgaaaatcat gatcagcctc tcgccggagc
    28321 gcaaatcctg gtaactttgc tccacccgct gaccagagag cgtcgccacc tgttcggcgt
    28381 aacggttgat gtcgccatta aggatcgcca ccagcgcttt gatgtgaaac atataccagc
    28441 tgattgccag atcgctttgc tccagagccg ccgaaagtgg cgcgagttta cgcagttcac
    28501 gattaaaatc agcgagtagc gtattaacca atggcgtatc gctgtgagtt ggcaaggcag
    28561 acatcactcc gaccagttga tgaataatcg cctgcggcgc gggggtggca atggcggcaa
    28621 caatcagtcg gttcatctct ttaagcaaaa tattatcttg cgcgtgagtg actttttgca
    28681 gatgccgcag ataacttaag ttttgataca gcgaacttaa cagcgcgtta cgctccagat
    28741 gccgcctttg cccgcgttcc agcattccgc cgacgctggt ttgcagttcg ttgctacgcg
    28801 tgatgatgcg cgtcaccagc ccggcttcct gctgcgctaa aggcgcgttc gccagttgtg
    28861 caagcgagct ttttaatgcc cgctgtgtct cctgcaaccg ctcggcttcg cctttatatt
    28921 ccagtgctcc caccacctgc gacaatcgca ctgccgccgt tgccacgttt gccgtgtcgc
    28981 gcgccagatt caggctgccg gtcatatcat ctacagtttg ctgcttcacc agctcttgca
    29041 tctggctggc atgacgaaag cccattaccg ccacgccgct gaccatcaac gtcaccgcca
    29101 ccaccagcaa attcaaaagc agcaatcgcc cgcgcgcgct ggcaaaaaag tgacgtccgg
    29161 ggtgcgccat atcacgctcc gtgaagaaaa atcgcaatat gacaaatatg aacggcttct
    29221 gacattttgc atttactttc ctgtgatgga gtgctgatat cgacaacctc gcaggagtcc
    29281 accatgaaca gcacgccggt tcttgagatg cgcaatattg ccaaagcctt cggcaaattt
    29341 tatgcgctca aaggggtgga tttgacggtc taccctggcg agatccatgc cctgatgggg
    29401 gaaaacggcg cgggaaaaag cacgctgatg aaggtgctgg cgggggcgta taccgccacc
    29461 agcggcgaga tcctgataga cggcaagccc tttcacattc gcacgccaaa agatgcctta
    29521 agcgccggta ttacgctgat ttatcaggag atgcagctgg cacccaattt gacggtggca
    29581 gaaaatattt tcctcggcag cgagctttcc cacggcgggc tggtgcagcg aaaagagatg
    29641 ctggtgcagg cgcaaagagt gatcgaccgc ctcggcgcgc agtttaacgc cagcgataag
    29701 gtcatgacgc tgaccattgc cgaacaacag caggtcgaaa tcgcccgcgc gctacatcgc
    29761 aacagccgca ttctggtgat ggacgaaccc accgctgccc tctcctcccg tgaaactcac
    29821 cgtctttttg aactgattat gcggctgcgc gatgaaggga tggcgattat ctacatcagc
    29881 caccgtatgg cggaagtgta tgagctttcc gatcgcgtca gcgtgctgcg cgacgggcaa
    29941 tacgttggca gcctgacccg cgataacctc aatgccgggg agctggtgcg gatgatggtc
    30001 ggcaggccac tgagcgatct gttcaataaa gagcgcgata tcccgctcgg taaagcccgc
    30061 ctgaatgttc accacctgac cgacggcggc aaagtccagc cgagctgcct gcaggtgctt
    30121 tccggcgaaa ttgttggcct cgccgggctg gtgggagccg gacgttccga actggcgcag
    30181 ttaattttcg gcgtgcggaa agcgacaggc ggaatgattg aagtcgatgg cgaaccggtg
    30241 gtgatccact ccccgcgcga agccatcgat cttggcattg gttttctcac cgaaaaccgc
    30301 aaagaacaag gcttattcct tgaaatggcg gcagtcgaaa acatcactat ggcaaccctg
    30361 gagcgcgatg ccagctgggg aatgctcaat cgcaaaaaag cgcaaaccat ttccgatgac
    30421 gccattaagt tgctcaacat tcgcgtgcct catgcccagg tacgcgcggg cgggctttcc
    30481 ggcggcaatc agcaaaaact gttgatctcc cgctgggtgg cgattggccc gcgcatttta
    30541 ctgctcgatg aacccacccg cggcgtggac gttggcgcaa aaagtgagat ctaccggatc
    30601 atgaacgaga tggcgcgcaa gggcgtggcg atcctgatga tctccagcga actgccggag
    30661 atagtgggaa tgagcgatcg cgtccatgtg atgcgcgaag gcagcattgc cggtgagcta
    30721 aatggcaaaa acatcaccca ggaaaacatt atgaccttag cgactggcgt caacgacgcc
    30781 cattcccagg cggtgacctc atgacgacct ctgcaaaaac ctctgccaaa aaaatgctga
    30841 tgagcgatct gatgcaaacg gtcggcattt tgcccatttt gatcctgatc gtagcggtat
    30901 ttggctttat cgcgccaaac ttttttaccg aaagcaacct gctaaatatt acccgccagg
    30961 cgtcgatcaa cattgtgctg gcggcgggga tgaccttcat cattttgacc ggtgggatcg
    31021 atctttctgt aggttcaatc ctcggcacca cggcggtggc ggcaatggtt gtctccctta
    31081 gcccggagtt cgccatgttg tctatcccgg cggcgctgat gctcggcctg ctgctggggc
    31141 tgttcaacgg cgcgctggtg gcctttgccg gattgccgcc gtttatcgtc acgcttggca
    31201 cctatacggc gctgcgcggg gcggcgtatc tgctggctga tggcaccacg gttatcaact
    31261 ccaacattaa tttcgagtgg ataggcaata actatctcgg gccggtcccg tggctggtgg
    31321 tgattgcgct ggcggtgatc gtgctgtgct ggtttattct gcgtcgcacc acgcttggcg
    31381 ttcatatcta cgccgtcggt ggcaatatgc aggcggcgcg tcttaccggc attaaggtct
    31441 ggctggtgct gctgtttgtt tatggcatga gcggcctgct ttcagggctt ggcgggatta
    31501 tgagcacctc gcggctgtac agcgccaacg gcaacttagg tatgggttac gaactggatg
    31561 ccattgcggc ggtgatcctc ggcggcacca gttttgtcgg cggcatcggc accatcaccg
    31621 gcacgctggt gggggcgcta attatcgcca cgctcaacaa tggcatgacc ttaatgggcg
    31681 tctcttactt ctggcagctg gtgatcaaag gggcggtgat catcattgcg gtgcttatcg
    31741 acaaataccg tacccgacat catcaaagtg cataacaaca ataccctaca catgaggaat
    31801 acagcatgag attgaagccc ttagtgaccg cgctctgtgc aggcgcgctg ctggccgcag
    31861 caccgttcgc ccaggcgaaa gatctgaaat cgataggcgt aacggtaggt gacctggcga
    31921 acccgttctt cgtacagatc accaaaggcg cggagctgga agcacgcaaa ctggcgggcg
    31981 ataacgtcaa agtgacgctg gtttccagcg gctacgatct gggtcagcag gtctcgcaga
    32041 tagacaactt tatcgccgct aacgtcgata tgatcatcct taacgctgcc gattcaaaag
    32101 ggatcggccc ggcggtgaaa cgggcaaaag acgccgggat cgtggtggtg gcggttgacg
    32161 tggcggcaga aggtgccgat gccaccatca cttccgataa cacccaagca ggcgaaatgg
    32221 cctgtaaata catcaccgat cgcctgaaag gcaaaggcaa cgtggtgatc atcaacggac
    32281 cgccggtgtc cgccgtgcaa aaccgcgtcg aaggctgcca gaccgaattt aaacgccatc
    32341 ctgacatcaa ggtgctctct gacaacaaga acgccaaagg cagtcgtgag ggcgggctgg
    32401 aggtgatgac cgggctgctg gcggcaaatc cgaagatcga cggcgtattt gcgattaacg
    32461 atccgacagc gatcggtgcc gatctggcag caaaacaggc gcaacgtaac gagttcttta
    32521 ttgtcggcgt ggatggaagc ccggacgccg aagaggcgct caagcgtgag aactcgttgt
    32581 ttgtcgctac tccggcgcaa gatccacagg tgatggcagc gaaagcggtt gagatcagtt
    32641 atgacatttt gcagggcaaa cctgcgccga aagagccggt gctgatcccg gtgacgatga
    32701 tcgataaaaa caacgtcggc aattacaaag gctggacggt gaaataagtc cacacgccgg
    32761 gcggcgatgc tgcccggcca ccgctaagga gaacgctttc gatgaaacgc tccgctataa
    32821 acaacattct cggccataca cggcaattct tttcgcaaca tgacgtacat ctgccgccct
    32881 ttgccagttt ctctcctgct caatggcagc agctcgacac cgccgcgtgg gaggtggttt
    32941 ttgatctcaa actcggctgg gatgttaccg cttttggtgg taacaacttt gcagcacaga
    33001 ggctgacgct gtttaccctg cgtaacggct cagcaaaagg aatgccgtat gtgaaatgtt
    33061 acgccgagaa gatcatgcat gtgcgcgatg cgcaggtcac gccaatgcat tttcactggc
    33121 gtaagcgtga ggacatcatc aatcgcggcg gcggcaattt gattgttgaa ctgtggaatg
    33181 ccgatagcaa cgagcaaaca gttgataccg acgttacggt ggtgatcgac ggctgccgcc
    33241 agactcatgt cgcaggcagt cagttacgcc tgtcccccgg cgaaagtatc tgcctgccgc
    33301 ccggcctgta tcacagcttt tgggcggaaa ccggttttgg cgatgttctg gttggcgaag
    33361 tctcttctgt caatgacgac gaccacgata accacttttt acaaccgctg gaccgctaca
    33421 acctgattga cgaagacgaa ccggcccagc tggtgttgtg taacgaatac cgccagttcc
    33481 tctgaataag gataacgtta tgccgttgat ttctctcgct gaagggctgg cacatgcccg
    33541 cgaacatcac tatgcgttag gtgcgtttaa tgtgctggac tcacactttt tacgcgcctt
    33601 gtttgctgct gccagacaag aacgttcgcc ctttattatc aacattgccg aagtacattt
    33661 taaatatatc tcgctggatt ccctggtcga gacagtgaaa tttgaagcgg cgcgtcaagc
    33721 gattccggtg gtattaaatc tcgatcacgg gctgcatttt gaatcggtgg cgcaagcgtt
    33781 gcggctggga ttcagctcgg taatgtttga tggctccacg ctggggtatg aagagaatat
    33841 ccgccagacg cgggaagtgg tgaaaatgtg ccatgccgtt ggcgtatcgg tagaagctga
    33901 actgggcgcg gtgggtggtg acgaaggcgg tgcgctttac ggtcatgccg atgaatctct
    33961 gtttaccgat ccgcagctgg cgcgtgattt tgtcgaccgt accggcattg acgcgctggc
    34021 cgtggcgatt ggcaatgcgc acggcaaata tcgcggcgaa ccgaaactcg atttcccgcg
    34081 cctcgacgct atccgccagc aggcggctat cccgctggta ttacacggcg gttcagggat
    34141 tagagatgcc gatttccgcc gcgccatcga actgggtatc cacaaaatca atttctacac
    34201 cggcatgtca caagcggcgc tggctgccgt tgaggcccga atggcgcacc gtcagccgat
    34261 gtatgacgag tttgccgagt tactactagc agtggaagcc gccatcagcg atacggttgc
    34321 acaacagatg cgtattttcg gcagtgcggg gcaggcatga tggaacgtaa agggattatc
    34381 gccgcaggca atatgctggt tgaccacgtc caccagatta tccagtggcc agagcgcggc
    34441 tggctggcgg agattaccca cagcgaacgc tccaccggcg gcgcgccgct taacgtcctg
    34501 ctgacactgg cgaaaatgcg caccgggttg ctgttacagg cggtcgggct aatcggtcag
    34561 gacagcgacg gcgattacat tatggcgatg cttgagcaat atcacgtcaa tcgtcagcat
    34621 gtgcagcgca ctacctttgc cccgacgtca atgtcgcagg taatgactga tccaagcggg
    34681 cagcggacgt ttttccattc gccaggtgca aacagacttt tggatcttcc ggcgttcgat
    34741 cagctcgata acaccatgaa gattttccat ctcggctatt tactgttgct cgacagcctg
    34801 gatatgccgg acgatgttta cggcacccgt agcgcacgcc tgctggcgca aatgcgtgat
    34861 actggctttg aaacctcact cgacctggtc tcccgcaagg gcgatccgcg ttatcagccg
    34921 ctggtgttgc ccgcgttgcg ccatctggat tacctggtaa ttaacgaact ggaggccggg
    34981 gagtttagcg ggctggaaat tcggttaccc aacggcgaac cgcatatcgc gcatattgct
    35041 gccgccgcgc gtgaactgtt agatgcgggc gtgcgccagc gtgtggtgat ccactgcccg
    35101 gaaggggcgt ggggagagac cccggaacag tgcggcgtgt ggatcccttc gcaaaagtta
    35161 gaacaacggg aaattatcgg cagcgtcggt gcgggcgatg tattttgtgc cggattttta
    35221 tatggctgcc atgaacgctg gacgcttacc gagagcatca aactggcaca cgcctgcgcg
    35281 cgagcaagcc tgttatgcgc caatgccatc gacggggcga aaacgctgga agagctgcaa
    35341 acagaaatgt cggattaatg cacgtcggca gcgaagacat accccagacc gcgcagagtt
    35401 ttaatcaaca tcggctgatg cgggttgagt tcgattttgc gtcgtaagcg catgattaat
    35461 acgtcgatgg tgcgatcaaa cacctccacg ctgtcgctat gagtgaacgc cagcagctgt
    35521 tcgcggctta atacccgacg ggcattttga gccagggcca gtaacaagcc atattcgccc
    35581 tgagtcagct cgacatgttg ttgtagcgga ctatgcagtt ggcagcgaat ggtatcgaga
    35641 taccagccat tgaatgacag cccggcgttt ttctcctcat taaccgccag cgcgccaacc
    35701 cgtcgcaaca cgctttcagt cgtgcgacca ccacccgtgg ggtgaacggc ttgcagatgt
    35761 aatcatccgc gcccatttcc agcccgacca ccacatcaga ctcactcccc attccggtca
    35821 acatcaccac aggcaatgcc ggacgcagtt tctggatttg ctgcaatacc ctcaggccgt
    35881 tggtatcggg cagcatcata tccagcacca ccagcgcaat gtcaggctcg ccctcaatac
    35941 gcgatatggc ctcctgtccg gtatggctga ctgaaaccgt aaagacatgt tcacttaaca
    36001 cgtcctgcaa cagtgcgcag atagccgtgt catcatccac caccagcacg acgggtttca
    36061 tggttacact ccgcatcatt catctaatta atcattgtgc gtacttcttg tcgaagtgaa
    36121 agcggaaagt aatataaata ataaaaatat aagtcaggtc acattattga tgatagtaat
    36181 aaatatgccg tgcttttccc tcttcagcac aaatgcctaa taaaatggcg cgttccgctc
    36241 acacttccac ttattaaagt cgcaaattcc caattatgat aaccccagct ttaaatgacg
    36301 tattttgcat ggattacata tttacgacac aaatgataaa tggagattta tatgaataac
    36361 tctcggttat tccgtttgag cagaattgtt attgcgttaa ctgccgccag cggcatgatg
    36421 gtaaataccg ctaacgcgaa agaggaagcg aaagccgcca ctcaatatac ccaacaggtt
    36481 aatcagaatt acgccaaatc attaccgttt agcgatcgtc aggattttga cgacgctcag
    36541 cgtggattta tcgctccgct gctggatgaa ggtattctgc gcgatgctaa tggcaaacca
    36601 tattatc
//
LOCUS       DAJVQH010000022        36520 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJVQH010000022 DAJVQH010000000
VERSION     DAJVQH010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.022"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(156..1391)
                     /locus_tag="OQM87_001254"
     CDS             complement(156..1391)
                     /locus_tag="OQM87_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1507463.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="OQM87_001255"
     tRNA            complement(1538..1614)
                     /locus_tag="OQM87_001255"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="OQM87_001256"
     tRNA            complement(1657..1743)
                     /locus_tag="OQM87_001256"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="OQM87_001257"
     tRNA            complement(1764..1839)
                     /locus_tag="OQM87_001257"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="OQM87_001258"
     tRNA            complement(1898..1974)
                     /locus_tag="OQM87_001258"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="OQM87_001259"
     CDS             complement(2077..3462)
                     /locus_tag="OQM87_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1507464.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OQM87_001260"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OQM87_001260"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCS1507465.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OQM87_001261"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OQM87_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCS1507466.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OQM87_001262"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OQM87_001262"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCS1507467.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OQM87_001263"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OQM87_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCS1507468.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRFGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OQM87_001264"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OQM87_001264"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCS1507469.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OQM87_001265"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OQM87_001265"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCS1507470.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OQM87_001266"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OQM87_001266"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1507471.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OQM87_001267"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OQM87_001267"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1507472.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OQM87_001268"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OQM87_001268"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCS1507473.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OQM87_001269"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OQM87_001269"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCS1507474.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAVNYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OQM87_001270"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OQM87_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCS1507475.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OQM87_001271"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OQM87_001271"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCS1507476.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OQM87_001272"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OQM87_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCS1507477.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OQM87_001273"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OQM87_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCS1507478.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OQM87_001274"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OQM87_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCS1507479.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="OQM87_001275"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="OQM87_001275"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCS1507480.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="OQM87_001276"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="OQM87_001276"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCS1507481.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OQM87_001277"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OQM87_001277"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCS1507482.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAVKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="OQM87_001278"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="OQM87_001278"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCS1507483.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OQM87_001279"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OQM87_001279"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCS1507484.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="OQM87_001280"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="OQM87_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCS1507485.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OQM87_001281"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OQM87_001281"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCS1507486.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OQM87_001282"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OQM87_001282"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCS1507487.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OQM87_001283"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OQM87_001283"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCS1507488.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OQM87_001284"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OQM87_001284"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCS1507489.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="OQM87_001285"
     CDS             complement(31267..32913)
                     /locus_tag="OQM87_001285"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCS1507490.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="OQM87_001286"
     CDS             complement(32916..32966)
                     /locus_tag="OQM87_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507491.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OQM87_001287"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OQM87_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCS1507492.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..35022
                     /locus_tag="OQM87_001288"
     CDS             33502..35022
                     /locus_tag="OQM87_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCS1507493.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="OQM87_001289"
     CDS             complement(35047..35385)
                     /gene="maoP"
                     /locus_tag="OQM87_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCS1507494.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35504..36343
                     /gene="hdfR"
                     /locus_tag="OQM87_001290"
     CDS             35504..36343
                     /gene="hdfR"
                     /locus_tag="OQM87_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCS1507495.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36439..36514)
                     /locus_tag="OQM87_001291"
     tRNA            complement(36439..36514)
                     /locus_tag="OQM87_001291"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36479..36481),aa:Trp,seq:cca)
BASE COUNT         8920 a         9993 c         9200 g         8407 t
ORIGIN      
        1 gccaccgcac gcaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gccatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttatgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacgcc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccaacccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cacgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcataacggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgccaaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcaac tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag tcaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 gcgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattca gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaattta ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagatcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctctcga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 gctgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag tcctgtgcaa aaattaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcgttagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttaccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcatgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgggc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatggttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca cgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acgggcgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattct ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtggtgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggagcacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggct attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgct ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccgatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggag tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcata gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgttcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgctat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcaaccg gctctcgggg
    34621 ccctttctcg accgcttcga tctctcactg gagatcccat taccaccccc cggcattttg
    34681 agtaaaacgg tagtgccggg agaaagcagc gccaccgtta aacaacgcgt aatggccgcc
    34741 agagagcgcc aatttaagcg gcagaataaa ctgaatgcct ggctggatag tccggaaata
    34801 cgccaattct gcaaacttga gagcgaagat gcgatgtggt tggaagaaac gctgatccat
    34861 ctggggttat cgattcgtgc ctggcagcgg ttactgaaag ttgcacgaac cattgctgat
    34921 attgatcagt ctgacattat cacacgtcag catttgcagg aggcagttag ctatcgtgcg
    34981 attgaccgtt tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc
    35041 tttttattaa tcgtcagaat cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc
    35101 ggaaagggtg tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg accacacgcg
    35161 ttctgcttct gtcactggct cacgttcgcc acggcatact gctacgaaga gtttctcttc
    35221 ctcggtaacc ggctcgcgtt tgccaagatc caactcattg aaggcataac catgacgctc
    35281 aagcagttgt gcctctttga tggtgaaatc accatgacga gagaatccac gtggataatg
    35341 tttattgtcg aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa
    35401 ttctttgacc gagctagtta tggcgcggag tattagttac gcttgacaga gtgtaaaaca
    35461 aaacatttaa atcataacga caaataattt tgcggagagc actgtggata cggaattgtt
    35521 aaaaactttc ctggaagtta gccgaacgcg tcactttggt cgagcggctg aatcgctcta
    35581 tctgacccag tcagcagtga gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa
    35641 ccttttcacc cgccacagaa acaatatccg tttaaccgct gccggtgaaa aactactgcc
    35701 ttatgcagaa acgctcatga gcacctggca ggccgcccgc aaggaggtgg cgcatacctc
    35761 acgacataac gagttttcta tcggtgccag cgcctcgttg tgggaatgta tgcttaatca
    35821 gtggttggga cgcttgtatc aaaatcagga tgcccataca ggcttacagt tcgaagcgcg
    35881 aattgcccaa cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg atcttcttat
    35941 taccactgaa gcgcccaaaa tggacgaatt tagtagtcag ttgctggggt atttcacttt
    36001 agcgctttat accagtgccc cttctaaact aaagggagat cttaactatc tgcgactgga
    36061 gtgggggcca gattttcaac agcatgaggc aggtttgatc ggtgctgacg aagttcccat
    36121 tctgacaacc agttctgctg aactggcaca gcaacagatt gcgatgctta atggttgcac
    36181 ctggctaccc gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac
    36241 aacactttca cggccgcttt atgccatatg gctgcaaaat agcgataaaa atgcgttgat
    36301 tcgcgatctt ttgaaaatta acgtgctgga tgaagtgtat taatctgaat ggctggcaag
    36361 gatgccggta gaaggattta cttcggagag gattatttca gataaaaaaa atccttagct
    36421 ttcgctaagg atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg
    36481 gagaccggtg ctctaccaat tgaactacgc ccctaattag
//
LOCUS       DAJVQH010000023        36211 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJVQH010000023 DAJVQH010000000
VERSION     DAJVQH010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.023"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(118..831)
                     /locus_tag="OQM87_001292"
     CDS             complement(118..831)
                     /locus_tag="OQM87_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1507496.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            complement(900..1589)
                     /gene="mutH"
                     /locus_tag="OQM87_001293"
     CDS             complement(900..1589)
                     /gene="mutH"
                     /locus_tag="OQM87_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCS1507497.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            2274..2804
                     /gene="rppH"
                     /locus_tag="OQM87_001294"
     CDS             2274..2804
                     /gene="rppH"
                     /locus_tag="OQM87_001294"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCS1507498.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            2817..5063
                     /gene="ptsP"
                     /locus_tag="OQM87_001295"
     CDS             2817..5063
                     /gene="ptsP"
                     /locus_tag="OQM87_001295"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1507499.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            5214..6089
                     /gene="lgt"
                     /locus_tag="OQM87_001296"
     CDS             5214..6089
                     /gene="lgt"
                     /locus_tag="OQM87_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCS1507500.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINSELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            6096..6890
                     /gene="thyA"
                     /locus_tag="OQM87_001297"
     CDS             6096..6890
                     /gene="thyA"
                     /locus_tag="OQM87_001297"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCS1507501.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            7074..7544
                     /locus_tag="OQM87_001298"
                     /pseudo
     CDS             7074..7544
                     /locus_tag="OQM87_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            7535..8098
                     /locus_tag="OQM87_001299"
     CDS             7535..8098
                     /locus_tag="OQM87_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1507502.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            8095..8502
                     /locus_tag="OQM87_001300"
     CDS             8095..8502
                     /locus_tag="OQM87_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCS1507503.1"
                     /translation="MNREKGVSSQALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            8487..8810
                     /locus_tag="OQM87_001301"
     CDS             8487..8810
                     /locus_tag="OQM87_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1507504.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPLANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            8823..12191
                     /gene="recC"
                     /locus_tag="OQM87_001302"
     CDS             8823..12191
                     /gene="recC"
                     /locus_tag="OQM87_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCS1507505.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            12367..15255
                     /gene="ptrA"
                     /locus_tag="OQM87_001303"
     CDS             12367..15255
                     /gene="ptrA"
                     /locus_tag="OQM87_001303"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCS1507506.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRMADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            15248..18790
                     /gene="recB"
                     /locus_tag="OQM87_001304"
     CDS             15248..18790
                     /gene="recB"
                     /locus_tag="OQM87_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCS1507507.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            18790..20616
                     /gene="recD"
                     /locus_tag="OQM87_001305"
     CDS             18790..20616
                     /gene="recD"
                     /locus_tag="OQM87_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCS1507508.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(20678..22009)
                     /gene="argA"
                     /locus_tag="OQM87_001306"
     CDS             complement(20678..22009)
                     /gene="argA"
                     /locus_tag="OQM87_001306"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCS1507509.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            22241..23494
                     /gene="amiC"
                     /locus_tag="OQM87_001307"
     CDS             22241..23494
                     /gene="amiC"
                     /locus_tag="OQM87_001307"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCS1507510.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            complement(23569..23645)
                     /locus_tag="OQM87_001308"
     tRNA            complement(23569..23645)
                     /locus_tag="OQM87_001308"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23609..23611),aa:Met,seq:cat)
     gene            complement(23679..23755)
                     /locus_tag="OQM87_001309"
     tRNA            complement(23679..23755)
                     /locus_tag="OQM87_001309"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23719..23721),aa:Met,seq:cat)
     gene            23964..25061
                     /gene="mltA"
                     /locus_tag="OQM87_001310"
     CDS             23964..25061
                     /gene="mltA"
                     /locus_tag="OQM87_001310"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCS1507511.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            25300..26106
                     /gene="tcdA"
                     /locus_tag="OQM87_001311"
     CDS             25300..26106
                     /gene="tcdA"
                     /locus_tag="OQM87_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCS1507512.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(26157..26600)
                     /gene="csdE"
                     /locus_tag="OQM87_001312"
     CDS             complement(26157..26600)
                     /gene="csdE"
                     /locus_tag="OQM87_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCS1507513.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(26600..27805)
                     /gene="csdA"
                     /locus_tag="OQM87_001313"
     CDS             complement(26600..27805)
                     /gene="csdA"
                     /locus_tag="OQM87_001313"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCS1507514.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            27997..28224
                     /locus_tag="OQM87_001314"
     CDS             27997..28224
                     /locus_tag="OQM87_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCS1507515.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            28575..29492
                     /gene="gcvA"
                     /locus_tag="OQM87_001315"
     CDS             28575..29492
                     /gene="gcvA"
                     /locus_tag="OQM87_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCS1507516.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            29511..29906
                     /locus_tag="OQM87_001316"
     CDS             29511..29906
                     /locus_tag="OQM87_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCS1507517.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            29899..30999
                     /gene="rlmM"
                     /locus_tag="OQM87_001317"
     CDS             29899..30999
                     /gene="rlmM"
                     /locus_tag="OQM87_001317"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCS1507518.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(31043..31774)
                     /gene="fucR"
                     /locus_tag="OQM87_001318"
     CDS             complement(31043..31774)
                     /gene="fucR"
                     /locus_tag="OQM87_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCS1507519.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSSALFHIRLKSHYAHKADIAREALAWIEERMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(31832..32254)
                     /gene="fucU"
                     /locus_tag="OQM87_001319"
     CDS             complement(31832..32254)
                     /gene="fucU"
                     /locus_tag="OQM87_001319"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCS1507520.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(32256..33675)
                     /gene="fucK"
                     /locus_tag="OQM87_001320"
                     /pseudo
     CDS             complement(32256..33675)
                     /gene="fucK"
                     /locus_tag="OQM87_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(33784..35559)
                     /gene="fucI"
                     /locus_tag="OQM87_001321"
     CDS             complement(33784..35559)
                     /gene="fucI"
                     /locus_tag="OQM87_001321"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCS1507521.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHTCGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(35592..>36211)
                     /gene="fucP"
                     /locus_tag="OQM87_001322"
     CDS             complement(35592..>36211)
                     /gene="fucP"
                     /locus_tag="OQM87_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCS1507522.1"
                     /translation="LQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTAC
                     WSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAPHKVLAAYALIA
                     MALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTIIG
                     GGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         8594 a         9036 c         9842 g         8739 t
ORIGIN      
        1 tacctatgat aatagtctgt ttataaatcg acgttatata tcttgaaagc aggaaaatct
       61 taaactgtga gggtaaaagg agtgagtacg ccctcccgca ggagagcgta cggagtatca
      121 gagcggattc tttttgttgc gaatcaagtt gaggctttca accgcaatag agaagaacat
      181 cgcgaagtag atgtaacctt tcggtacgtg gatgtcgaaa ctttccagaa tcagggtaaa
      241 gcccaccaga atcaggaaag agagcgccag catttttacc gaaggatggc gttcgacaaa
      301 atcaccaatc gagcgcgcgg cgaacatcat cacgcctacg gcaatcacca cggctgccat
      361 cataataaac aggtgatctg acagacccac agcggtaatc accgagtcga ggctgaagat
      421 aatatccagc agcataatct gcacgatagc gccgaggaat gatgaaacgc gtgttttcag
      481 cccttcttct tcaccttcaa tggattcgtg gatttccttg ctggctttcc agataaggaa
      541 caagccaccc agaagcagaa tcaaatcacg ggcagaaatt tcctgactga atattgtaaa
      601 aagcggattt gtcaggcgcg taacccaaga gattgatgcc agcagagcca gacgcataac
      661 catggctccc gccaacccca gacggcgcgc atgagcacgt tgtgctgtgg gaagctttgc
      721 caccaccaga gaaaggaaaa taatattgtc gatcccaaga acgatctcca gcagcgtcag
      781 cgtaccgagc gcaagccagg cgttaggatc ggttatccat gcaaataaca tctaaaaagc
      841 cctgccaaaa aaatgaagcg gtaattatat gcccggaaag cggcaggtca aagcgatggc
      901 tactggatca gaaaatgacg ggccagtagt gcactggtga aattcttctt caaataaaag
      961 ccgcgtggca gcgtcagaat ccgttcgccc cgggcaccaa tggcttcggt aagcgctttc
     1021 gcattcgctg ctttcggtct tatctgtaaa tattccccgt gacgagcggt gatccgctca
     1081 acctgaccga gaacaatcat atccattaat tcttcccagt cttcgcgtag ctgtcggtct
     1141 tcctcttcat tcgggctcca cagcaacggt gatcctacgc gacgctgcgc cagcgggatg
     1201 ctgcgctcgc cttcaaccgg tatccacagt acgcgtttga gcttgtggcg cacatggctg
     1261 gtttcccagg tcaccccgct attacccgtt aacggggcaa cacaaacgaa tgttgtttcc
     1321 agcggacgac caagactatc cacagggata gttttaagtt ccacgcccag agcagcaaaa
     1381 tcttgctcag gtttactccc tgcgctggca cctagccaga tctccagtaa cacgccaatc
     1441 cagcccttat cgcgttttaa attctctggc gtaaccagcc cggcaagtgc cgccagttct
     1501 cccaatgtat aaccagaaag ttgctgtgct tgcgctaaca actgttcttc agtttcggga
     1561 ggagagagca gtgggcgagg ttgggacatg tcatgatgcc ttggttaaaa aacaaacagc
     1621 aattatgtgc cgaatattcg cagtgtaact ttttccacat gtgttgttca attctctgat
     1681 ttatattgct ttttaggctg ccggtaatcg agttgcgcaa cactgcaata ggttttccaa
     1741 atctggtcac tgaaaataaa caggatctta caccatgtta tccacagaaa gctgggataa
     1801 ctgtgaaaaa cctccactac tgttttcatt tacagccttg acgtgcgacg aaactccagt
     1861 ttttagtggt tcagtgctta acttagtggc acattctgtg gataaatacg gcattggttg
     1921 atctttcgcc aacccgtaaa tgagaggtgt gacctgggtc acgaattact ctttttcatt
     1981 actcaaatta catgtaatgc attgactaat aatgaatatt ttaaaatgtc tatatcgggc
     2041 ttattcagaa tgctccgggt tttcccgtag taattccgta gttcttcaca actctatcca
     2101 cagaaaaggt gaataaaaac ggctatccac cccttcctct gtttataact ctgcacataa
     2161 ctgtgagtta ttcaattgtt attagtgtgt aatgccctgc agagagtggt ttaccgtctt
     2221 cctggagtat gaaacaatca ttcgtatata aagctttatt ttgaggtagt ccggtgattg
     2281 atgacgatgg ctaccgccca aacgtaggta tcgtgatttg taatcgccag gggcaggtaa
     2341 tgtgggcccg gcgatttggt cagcactcct ggcaatttcc gcaaggcgga atcaaccccg
     2401 gagaatccgc agagcaggcg atgtaccgtg aattgtttga agaagtagga ttaagccgca
     2461 aagacgttcg aatccttgct tcaacgcgta actggttgcg ctacaaatta ccgaaacgtt
     2521 tggtgcgttg ggacacgaag ccggtttgta tcggccaaaa acaaaaatgg tttctcttgc
     2581 agctggtaag cggcgatgca gaaatcaata tgcaaaccag cagtacacca gagtttgacg
     2641 gctggcgatg ggtaagttac tggtatccgg tcagacaggt ggtgtcattt aaacgtgatg
     2701 tctaccgtag ggtaatgaaa gagttcgcga gtgtggtgat gtcactgcag gaaaatacgc
     2761 caaaaccaca aaacgcatct gcttatcgac gtaaaagagg ttaagtcacg ccaattatgc
     2821 tcactcgcct gcgcgaaata gtcgaaaagg tagccagcgc accacgcctg aatgaggcgt
     2881 taaatattct ggttaccgac atctgtcttg cgatggatac cgaggtctgt tcggtctacc
     2941 tggccgatca tgatcgacgt tgttactacc tgatggcgac ccgggggctg aaaaaaccac
     3001 gcggtcgcac tgtaacgctc gcgtttgatg aagggatcgt cggcctggtt ggcaggctgg
     3061 cggaaccgat aaaccttgca gatgcgcaaa agcaccccag cttcaaatac atcccctccg
     3121 taaaagaaga acgtttccgc gcgtttttag gcgtaccaat tattcaacgt cgccagttgc
     3181 ttggtgtact ggtggtacag caacgagagt tgcgccagta tgacgaaagt gaagaatcct
     3241 tcctggtgac gcttgccacc cagatggcag ctattctttc tcagtcgcag ttgactgccc
     3301 tgtttgggca atatcgccag acgcgaatcc gcgcattacc ggcagcacct ggtgtggcga
     3361 ttgccgaagg ctggcaggat gccacgttac ctttaatgga acaggtgtat caggcatcaa
     3421 cgctggatcc ggctctggaa cgcgaacgac tgaccggggc gctggaagaa gcggcaaacg
     3481 agtttcgccg ctacagcaaa cgctttgccg ccggagcaca aaaagaaacg gcggctattt
     3541 tcgatcttta ctcgcacctg ctttcggaca cccggctgcg tcgcgaattg tttgccgagg
     3601 ttgataaagg ctcggtggca gagtgggcgg taaaaacggt cattgaaaaa tttgccgaac
     3661 agtttgccgc gctaagcgat aactatctca aagagcgggc tggcgattta cgtgcgctgg
     3721 gtcagcgatt gctgtttcat cttgatgacg ctaatcaagg gccgaacgcc tggccggaac
     3781 gtttcattct ggtggcagat gaactgtcag cgacaactct tgctgagctg ccccaggatc
     3841 gcttagtcgg tgttgtcgtg cgagatggcg cagccaactc ccatgctgcg atcatggtac
     3901 gtgcgctggg gatccctacc gtgatgggcg cggatattca gccttcggtg ctgcatcgta
     3961 ggacgctgat cgttgatggc tatcgcggtg aattgctggt cgatccggag ccggtactgc
     4021 tgcaagaata tcagcggcta attagtgaag agattgagct tagccgtctg gcggaagatg
     4081 acgtcaattt acccgcgcag ttaaaaagcg gtgagcgtat aaaagtcatg ctcaatgctg
     4141 gtttaagccc ggaacatgaa gaaaaactgg gcagccgtat tgatggcatc ggtctttatc
     4201 gcactgaaat cccattcatg ctgcaaagtg gtttcccgtc ggaagaagaa caggtggcgc
     4261 agtatcaggg gatgctgcaa atgttcaatg ataaacccgt caccttgcgt acgctggatg
     4321 tcggagcaga taagcagctg ccttacatgc cgatcagcga agagaatcca tgcctgggtt
     4381 ggcgtggtat tcgcattacg ctcgatcagc cggagatctt cttgatccag gtgcgggcga
     4441 tgctgcgtgc taatgccgct acgggcaacc tgaatattct gttgccgatg gtcaccagcc
     4501 tcgatgaagt tgacgaagca cgccgcctga ttgaacgtgc cggacgtgaa gtcgaggaga
     4561 tgatcggtta cgaaattccc aaaccacgta tcggcatcat gctggaagtg ccgtcaatgg
     4621 tatttatgct gccgcatctg gcaaagcggg tcgatttcat ctctgttggc accaacgatc
     4681 tgactcaata cattctggcc gttgatcgca acaatacccg ggtggctaac atttacgaca
     4741 gtcttcatcc tgcaatgtta cgagctctgg cgatgatcgc ccgggaagcg gaaatacatg
     4801 gtatcgatct ccgtttgtgc ggtgaaatgg cgggcgatcc catgtgtgtg gcaatcctca
     4861 ttgggcttgg gtatcgccat ctgtctatga acggacgttc tgtagcgcgg gtaaaatacc
     4921 tgctgcggcg cattgatttt gccgaagcag aaaatcttgc gcagcgtagt ctggaagcgc
     4981 aactggcgac cgaagttcgt catcaggttg cagcctttat ggagcgtcgc ggcatgggcg
     5041 ggctgattcg cggagggtta tagcgcggat catatacata tcttttaacg gtatccggca
     5101 accagccagg tccccttgtg ctattattcg cacctttgga gcgcctgaaa cctgcggcgc
     5161 gcatttcaat cgctgttctc tttcagcgaa ataacaagaa cttgtggtga cagatgacca
     5221 gtagctatct gcattttccg gagtttgatc cggtcatttt ctcaatagga cccgtggcgc
     5281 ttcactggta cggcctgatg tatctggtgg gtttcatttt tgcaatgtgg ctggcaacac
     5341 gtcgggcgaa tcgtccgggc agtggctgga ccaaaaatga agttgaaaac ttactctatg
     5401 cgggcttcct cggcgtcttc ctcgggggac gtattggtta tgttctgttc tacaatttcc
     5461 cgcagtttat ggccgatccg ctgtatctgt tccgtgtctg ggacggcggc atgtctttcc
     5521 acggcggcct gattggcgtt atcgtggtga tgattatctt cgcccgccgt actaaacgtt
     5581 ccttcttcca ggtctctgat tttatcgcac cactcattcc gtttggtctt ggtgccgggc
     5641 gtctgggcaa ctttattaac agtgaattgt ggggccgcgt tgacccgaac ttcccgtttg
     5701 ccatgctgtt cccaggctcc cgtacagaag atattttgct gctgcaaacc aacccgcagt
     5761 ggcaatccat tttcgacact tacggtgtgc tgccgcgcca cccatcacag ctttacgaac
     5821 tgctgctgga aggtgtggtg ctgtttatta tcctcaacct gtatattcgt aaaccgcgcc
     5881 caatgggagc tgtctcaggc ttgttcctga ttggctacgg cgcgtttcgc atcattgttg
     5941 agtttttccg ccagcccgac gcgcagttta ccggtgcctg ggtgcagtac atcagcatgg
     6001 ggcaaattct ttccatcccg atgattgtcg cgggtgtgat catgatggtc tgggcatatc
     6061 gtcgcagccc acagcaacac gtttcctgag gaaccatgaa acagtattta gaactgatgc
     6121 aaaaagtgct cgacgaaggc acacagaaaa acgaccgtac cggaaccgga acgctttcca
     6181 tttttggtca tcagatgcgt tttaacctgc aggatggatt cccgctggtg acaaccaaac
     6241 gttgccacct gcgttccatc atccacgaac tgctgtggtt cctgcagggc gatactaaca
     6301 ttgcttatct acacgaaaac aatgtcacca tctgggacga atgggccgat gaaaatggcg
     6361 acctcgggcc agtgtatggt aaacagtggc gcgcctggcc aacgccagat ggtcgtcata
     6421 ttgaccagat tactacggta ctgaaccagc tgaaaaacga cccggattcg cgccgcatta
     6481 ttgtttcagc gtggaacgta ggcgaactgg ataaaatggc gctggcaccg tgccatgcat
     6541 tcttccagtt ctatgtggca gacggcaaac tctcttgcca gctttatcag cgctcctgtg
     6601 acgtcttcct cggcctgccg ttcaacattg ccagctacgc gttactggtg catatgatgg
     6661 cgcagcagtg cgatctggaa gtgggtgatt ttgtctggac cggtggcgat acgcacctgt
     6721 acagcaacca tatggatcaa actcatctgc aattaagccg cgaaccgcgt ccgctgccga
     6781 agttgattat caaacgtaaa cccgaatcca tcttcgacta ccgtttcgaa gactttgaga
     6841 ttgaaggcta cgatccacat ccgggcatta aagcgccggt ggctatctaa ttacgaaaca
     6901 tactgccaga gccgacgcca gtgtgcgtcg gtttttttac cctccgttaa attcttcgag
     6961 acgccttccc gaaattttgc aacgtcctgc aacggcgtaa atagtccgga agatgcgccg
     7021 aagaaataga aactggccct cgtctttttt tctcctcgct ccatactgcc ggcatgaaaa
     7081 cacaacgtgg ttatacgctg attgaaacgc tggtcgcgat gctgattttg gtcatgctaa
     7141 gcgcaagtgg actctatggc tggcaatact ggcagcagtc gcaacggctt tggcaaaccg
     7201 ccagctaggc gcgggactat ttgctctatt tacgtgaaga tgccaactgg cataaccgcg
     7261 accacagtat cagcgttatc agggagggga cgttatggtg ccttgtgagt tccgctgctg
     7321 gggccaatac ctgtcatggc agttcaccat tggtctttgt gccacgctgg cccgaagtcg
     7381 aaatgagcga cctgacacct tcgcttgctt tctttggcct gcgcaatacc gcatgggccg
     7441 ggcatattcg cttcaaaaac tcaacgggcg agtggtggct ggtggtttcg ccgtggggaa
     7501 gactccggct ttatcagcaa ggagaaacag aaggatgcct gtaaaagagc aaggtttttc
     7561 tctgctggaa gtgttgattg ctatggcgat cagtagcgta ttgttgctgg gggctgcacg
     7621 ctttctgcct gcgttacagc gtgaaagttt aacgagcact cgtaagctgg cactggaaga
     7681 tgaaatctgg ctgcgggtat ttaccgtcgc gaagcatctc cagagggcgg gttattgtca
     7741 tggcagctgt accggcgaag ggctggaaat tgtcggacag ggtgactgta tcattgtgca
     7801 gtgggatgcg aacagtaacg gtatctggga tcgcgaaccg gtaaaagagt ccgaccagat
     7861 tggatttcgt ctgaaggagc atgtgctgga aacgctacgc ggtgcgacat cctgtgaagg
     7921 taagggctgg gataaagtca ctaatccgga tgccatcatt atcgacactt ttcaggtcgt
     7981 acgtcaggat gtcagcggct tctcgccggt gttgacggtt aatatgcgtg ctgccagtaa
     8041 gtctgaaccg caaaccgtgg tggatgccag ctatagcgtg acaggattca acctgtgaac
     8101 cgcgaaaaag gtgtttcgtc acaggctctg gtcctgatgc tgctggtttt gggtagcttg
     8161 ctattgcaag gaatgagtca gcaggatcgc agttttgcct ctcgcgtgag catggaaagt
     8221 cagtcattgc gccgccaggc catcgttcag tcggcgctgg cgtggggaaa aatgcactcc
     8281 tggcagacgc agccagcagt tcagtgctcg cagtacgctg gaaccgatgc ccaggtttgt
     8341 ttgcgtttac tggcagataa tgaagcctta ttgattgccg gttatgaagg cgtttcgttg
     8401 tggcgaacag gcgaagtcat tgatggaaaa attgtttttt cgccacgcgg ctggagcgat
     8461 ttttgtccgc tgaaagaggg ggcattatgt cagcttccct gaagaatcaa caaggcttta
     8521 gcctgccgga ggtaatggtt gcgatggtgc tgatggtgat gattgtcact gcgttatcgg
     8581 gtatccagcg aacattaatg aacagtctcg ccagcagaaa ccagtaccaa cagctctggc
     8641 ggcatggttg gcagcaaacg caactgcgcg cgatttcgcc acttgccaac tggcaggtca
     8701 accgaatgca gacatcgcag gcgggatgtg tcagcatcag cgttacgcta gtttcacccg
     8761 ggggcagaga aggcgagatg acccgcctgc attgcccgaa tcgtcagtag tcaggagccg
     8821 ctatgttaag ggtctaccat tccaatcgtc tggacgtgct ggaagcgttg atggagttta
     8881 ttgtcgaacg cgaacggctg gacgatcctt tcgaaccaga gatgattctg gtgcaaagta
     8941 ccggtatggc gcagtggctg caaatgaccc tgtcgcaaaa gtttggtatt gcggcaaaca
     9001 ttgattttcc gctgccagcg agctttatct gggatatgtt cgtccgggtg ttaccggaaa
     9061 tccccaaaga gagcgccttt aacaaacaga gcatgagctg gaaactgatg actctgctgc
     9121 cgcaattgct ggagcgcgaa gactttaccc tgttgcggca ttatctgact gacgatagcg
     9181 acaagcgaaa actgttccag ctttcctcaa aagcggcgga cctgtttgac cagtatctgg
     9241 tctatcgtcc ggactggctg gcacagtggg aaacaggaca tttggttgaa gggctgggag
     9301 aagcacaggc ctggcaagcc ccgttgtgga aggcgctggt ggaatatacc catgaactcg
     9361 gacaaccgcg ctggcaccgc gcgaatctct atcagcgctt tatcgaaacg ctggagtccg
     9421 cgacgacctg cccgccgggg ttaccttcgc gcgtctttat atgcggtatt tccgcgttac
     9481 cgcctgttta tctccaggcg ctccaggcgc tgggtaaaca tattgaaatc catctcctgt
     9541 ttaccaaccc ctgccgttat tactggggcg atattaaaga tcctgcttat ctggcgaaac
     9601 tgctgactcg tcagcgtcga cacagttttg aagatcgcga attgccgcta tttcgcgata
     9661 gcgaaaatgc cggacagctc tttaacagcg atggtgaaca ggatgtcggc aacccgctgc
     9721 tggcctcatg ggggaagctt gggcgcgact acatttatct cctttctgac ctggagagca
     9781 gccaggagct ggacgctttt gtcgatgtga cgccagataa cctgctgcat aacattcagt
     9841 ctgacattct ggaactggaa aaccgcgccg ttgcgggtgt gaacatcgaa gagttttccc
     9901 gtagcgataa caaacgcccg cttgatccac tggatagcag tatcacattc cacgtttgcc
     9961 atagcccgca gcgtgaagtt gaagttttac acgatcgctt gctggcgatg ctggaggaag
    10021 ccccgacact tactccgcgc gacatcatcg tgatggtggc tgatatcgac agctacagtc
    10081 cgtttattca ggcagtgttt ggtagcgcac ctgcggatcg ttacctgcct tacgccattt
    10141 ccgaccgtcg tgcgcggcag tcacatccgg tactggaagc gtttatcagc ctgttatcac
    10201 tgcctgacag tcgctttgtg tcagaggatg tgctggcgct gctggatgtg ccggtgctgg
    10261 cggcgcggtt tgacatcacc gaagaagggc tgcgttattt acgccagtgg gtcaacgaat
    10321 ccggcattcg ttgggggata gatgacgaca acgttcgcga gctggaactc cccgccaccg
    10381 gacaacacac ctggcgattt ggcctgacgc gtatgttgct gggctacgcg atggagagcg
    10441 cgcagggcga gtggcaatcg gttctacctt atgatgaatc gagcggctta attgcagaac
    10501 tggtggggca tctggcttca ctgctaatgc agctaaacat ctggcgtcgc gggctggcac
    10561 aggagcgtcc gctagaagag tggttgccgg tttgtcgcga tatgctcaac gccttcttcc
    10621 tgccggatgc ggaaaccgaa gcggcgatga cgctgatcga acaacaatgg caggcgatta
    10681 tcgccgaagg tttaggtgcg cagtatggcg acgcggtgcc gctgtcacta ttgcgtgatg
    10741 aactggcaca gcgtctggat caagaacgta tcagccagcg ttttctcgcc ggaccggtta
    10801 atatttgtac tctgatgcca atgcgttcaa ttccgttcaa agtggtttgc ctgctgggaa
    10861 tgaacgacgg cgtttatcca cgtcagcttg cgccattggg ctttgacctg atgagccaga
    10921 aaccgaagcg tggcgaccgt agccgtcgcg atgacgaccg ctacctgttc ctggaagcgt
    10981 taatttccgc gcagcaaaaa ctctatatca gctatatcgg gcgttccatt caggataaca
    11041 gtgaacgttt cccgtcggta ctggtgcagg aactgatcga ctacatcggg caaagtcatt
    11101 atctaccggg cgatgaagcg ctcaactgtg atgaaagcga ggcaagggta aaagcgcatc
    11161 ttacttgcct ccatacccgg atgccgtttg atccgcaaaa ctaccagcca ggcgaacgac
    11221 aaagctatgc tcgtgaatgg ctacctgcgg ccagccaggc tggtaaagca cattctgaat
    11281 ttgttcagcc gctgccgttt accttaccgg aaaccgtgcc gctggaaacg ctacaacgat
    11341 tctgggcaca tccggtgcgg gcgttcttcc agatgcgttt gcaggtgaac ttccgtactg
    11401 aagacagcga aatcccagac accgagccat ttattctgga aggacttagc cgttatcaaa
    11461 tcaatcagca gttattgaat gcactggttg agcaggatga tgccgaacgc ttgttccgcc
    11521 gcttccgggc ggcaggggat ttaccgtatg gcgcttttgg tgaaattttc tgggaaacac
    11581 agtgccagga gatgcagcag cttgccgaca gagtcattgc ctgtcgccag ccggggcaga
    11641 gtatggaaat tgatctcgcc tgcaacggtg tgcagataac tggctggttg ccgcaggtgc
    11701 agccggatgg cctgttgcgc tggcgtccct ctttattaag tgtggcgcag ggaatgcaac
    11761 tttggctgga acaccttgtc tactgtgcca gcggtggtaa tggcgaaagt cgcctttttc
    11821 tacgcaaaga cggtgagtgg cgttttccgc cgcttgcagc cgaacaggct ttgcattacc
    11881 tctcacaact gattgagggg tatcgtgaag gaatgtccgc gccattgctg gtgttacctg
    11941 aaagtggcgg cgcgtggcta aaaacctgtt atgacgcgca aaacgatgcc atgctggatg
    12001 acgattctac gttgcaaaaa gcccgtacga aattccttca ggcttacgaa ggcaacatga
    12061 tggtgcgtgg cgaaggtgat gatatctggt atcaacggct ctggcggcaa ttaacaccag
    12121 agacaatgga ggccatcgtt gaacagtcgc aacgtttcct gttaccgctg tttcgcttta
    12181 atcagtcatg agtgttgtat aaaaattgcg caatctatcc gcttacttta tgatgcgcac
    12241 cagtcacgga ctgatggtta tataaacata ggctgactcg tgcagcacaa gattaaattc
    12301 tggcagatga tttgcgttaa cctgttgaat ctgtccagaa aataaagttg attatgaggt
    12361 ccgtgaatgc cccgcagcac ctggttcaaa gcattattgt tgtttgttgc cctctgggca
    12421 ccattaagtc aggcagaaac gggatggcag ccgattcagg aaaccatccg taaaagtgat
    12481 aaagataacc gccagtatca ggctatacgt ctggataacg gtatggtggt cttgctggtt
    12541 tctgatccgc aggcagttaa atcgctctcg gcgctggtag tgcccgttgg gtcgctggaa
    12601 gatcccgagg cgtaccaggg gctggcacat taccttgaac atatgagtct gatggggtcg
    12661 aaaaagtacc cgcaggctga cagtctggcc gaatatctca aaatgcacgg cggtagtcac
    12721 aatgccagca ctgcgccgta tcgcacggct ttctatctgg aagttgagaa cgacgccttg
    12781 cccggtgcgg tagaccgcat ggccgatgct attgcagaac ccttgctcga caagaaatac
    12841 gccgaacgtg agcgtaatgc agtgaacgct gaattaacca tggcgcgtac gcgtgacggg
    12901 atgcgcatgg cacaggtcag cgcagaaacc attaacccgg cacaccccgg ttcaaagttt
    12961 tctggtggta acctcgaaac tttaagcgac aaacctggta atccggtgca gcaggcgctg
    13021 aaagatttcc acgagaagta ctattccgcc aatttgatga aggcggttat ttacagtaat
    13081 aaaccgctgc cggagttggc aaaaatggcg gcggacacct ttggtcgcgt gccgaacaaa
    13141 gagagcaaaa aaacggaaat caccgtgccg gtagtcaccg acgcgcaaaa gggcattatc
    13201 attcattacg tccctgcgct gccgcgtaaa gtgttgcgcg ttgagtttcg catcgataac
    13261 aactcagcga agttccgtag taaaaccgat gaattgatta cctatctgat tggcaatcgc
    13321 agcccaggta cactttctga ctggctgcaa aagcagggat tagttgaggg cattagcgcc
    13381 aactccgatc ctatcgtcaa cggcaacagc ggcgtattag cgatctctgc gtctttaact
    13441 gataaaggcc tggctaatcg cgatcaggtt gtggcggcaa tttttagcta tctcaatctg
    13501 ttacgtgaaa aaggcattga taaacaatac ttcgatgaac tggcgaatgt gctggatatc
    13561 gacttccgtt atccgtcgat cacccgtgat atggattacg tcgaatggct ggcagatacc
    13621 atgattcgcg ttcctgttga gcatacgctg gatgcagtca atattgccga tcggtacgat
    13681 gctaaagcag taaaggaacg tctggcgatg atgacgccgc agaatgcgcg tatctggtat
    13741 atcagcccga aagagccgca caacaaaacg gcttactttg tcgatgcgcc gtatcaggtc
    13801 gataaaatca gcgcacaaac tttcgccgac tggcagaaaa aagccgccga cattgcgctc
    13861 tctttgccag agcttaaccc ttatattcct gatgatttct cgttgattaa gtcagagaag
    13921 aaatacgacc atccagagct gattgttgat gagtcgaatc tgcgcgtggt gtatgcgcca
    13981 agccgttatt ttgccagcga gcccaaagct gatgtcagcc tgattttgcg taatccgaaa
    14041 gccatggaca gcgcccgcaa tcaggtgatg tttgcgctca atgattatct cgcagggctg
    14101 gcgcttgatc agttaagcaa ccaggcgtcg gttggtggca taagtttttc caccaacgct
    14161 aacaacggcc ttatggttaa tgctaatggt tacacccagc gtctgccgca gctgttccag
    14221 gcattgctcg aggggtactt tagctatacc gctacggaag agcagcttga gcaggcgaag
    14281 tcctggtata accagatgat ggattccgca gaaaagggta aagcgtttga gcaggcgatt
    14341 atgcccgcgc agatgctctc gcaagtgccg tacttctcgc gagatgaacg gcgtaaaatt
    14401 ttgccctcca ttacgttgaa agaggtgctg gcctatcgcg acgccttaaa atcaggggct
    14461 cgaccagagt ttatggttat cggcaacatg accgaggccc aggcaacaac gctggcacgc
    14521 gatgtgcaaa aacagttggg cgctgatggt tcagagtggt gtcgaaacaa agatgtagtg
    14581 gtcgataaaa aacaatccgt catctttgaa aaagccggta acagcaccga ctccgcactg
    14641 gcagcggtat ttgtaccgac tggctacgat gaatacacca gctcagccta tagctctctg
    14701 ttggggcaga tcgtacagcc gtggttctac aatcagttgc gtaccgaaga acaattgggc
    14761 tatgccgtgt ttgcgtttcc aatgagcgtg gggcgtcagt ggggcatggg cttccttttg
    14821 caaagcaatg ataaacagcc ttcattcttg tgggagcgtt acaaggcgtt tttcccaacc
    14881 gcagaggcaa aattgcgagc gatgaagcca gatgagtttg cgcaaatcca gcaggcggta
    14941 attacccaga tgctgcaggc accgcaaacg ctcggcgaag aagcatcgaa gttaagtaaa
    15001 gatttcgatc gcggcaatat gcgcttcgat tcgcgtgata aaatcgtggc ccagataaaa
    15061 ctgctgacgc cgcaaaaact tgctgatttc ttccatcagg cggtggtcga gccgcaaggc
    15121 atggctattc tgtcgcagat ttccggcagc cagaacggga aagccgaata tgtgcatcct
    15181 gaaggctgga aagtgtggga gaacgtcagc gcgttgcagc aaacaatgcc cctgatgagt
    15241 gaaaagaatg agtgatgtcg ccgagacact agatcctttg cgcttgccct tacagggcga
    15301 gcgcctgatt gaagcctctg ccggcacagg caaaaccttt acgattgcgg cgctctattt
    15361 gcgcctgtta cttggactag gcggttccgc cgcctttccc cgcccgctga tcgttgaaga
    15421 actgctggtg gtcaccttta ccgaggctgc cacggcagaa ttgcgcggtc gtatccgtag
    15481 caatatccac gagttgcgca tcgcctgtct gcgtgaaacc accgacaatc cactgtacga
    15541 acgcctgctg gaagagatcg acgataaagc gcaagccgcg cagtggttgt tgttagccga
    15601 acggcagatg gatgaagcgg cagtctttac tattcacggc ttttgccagc gcatgctcaa
    15661 cctgaatgcc tttgaatccg gcatgctgtt tgagcagcag ctgattgaag atgagtctct
    15721 gctacgctac caggcctgcg ccgatttctg gcgtcgccac tgctacccgc tgccgcgtga
    15781 aatagcccag gtcgtctttg aaacctggaa agggccgcag gcgttgctgc gcgatattaa
    15841 tcgttatctg caaggcgaag cgccggttat caaagcaccg ccgcccgatg atgaaacgct
    15901 ggcttcccgc cacgcgcaaa ttgtggcgcg tattgatacg gtaaaacagc agtggcgcga
    15961 cgcagtgggt gaactggatg cgctgatcga atcttctggt attgatcgac gcaagtttaa
    16021 ccgtagcaat caggctaaat ggatcgaaaa gatcagcgct tgggcagaag aagagacaaa
    16081 cagttatcag ttgccggagt cgctggaaaa attctcccag cgtttcttag aagatcgcac
    16141 gaaggccggg ggggaaaccc cgcgacatcc actgtttgag gtgatcgatc aactgcttgc
    16201 agaaccattg tcgatccgcg atctggtgat cacccgagca ttggctgaga tccgcgaaac
    16261 agtagcgcgt gaaaaacgcc gccgtggcga attgggtttt gatgacatgt taagtcggct
    16321 cgattccgcg ctgcgtagcg aaagcggcga ggtgttggca gcggcgatcc gtacgcgatt
    16381 cccggtggca atgatcgatg aatttcagga taccgacccc cagcagtacc gaatttttcg
    16441 ccgtatctgg caccatcagc cggaaaccgc attgttgctg attggcgacc cgaagcaggc
    16501 catatatgca ttccggggtg cggatatctt cacttatatg aaggcgcgta gtgaagttca
    16561 cgcccactac actttagaca ccaactggcg ctccgcacct ggaatggtga acagtgtgaa
    16621 caagcttttc agccagactg atgacgcgtt catgtttcgc gaaataccgt ttattccagt
    16681 gaaatcagcc gggaaaaatc aggcgttacg ttttgtattt aaaggtgaaa cgcagcctgc
    16741 gatgaaaatg tggctgatgg aaggcgaaag ctgcggcgtt ggcgactatc aaagtaccat
    16801 ggcgcaggta tgtgctgcgc aaatccgcga ctggctacaa gccggacagc ggggcgaagc
    16861 gttgctgatg aacgacgacg acgcgcgtcc ggtgcgtgct tcggacatca gcgtgctggt
    16921 gcgcagccgc caggaggccg cccaggtgcg cgatgcctta acgttgctgg aaatcccttc
    16981 cgtttacctt tcgaaccgcg acagtgtttt tgaaactctg gaagcgcagg aaatgctttg
    17041 gttgttgcag gcggtgatga cgcccgaacg tgagaacacc ctgcgcagtg cgctggcaac
    17101 gtcaatgatg gggctgaatg cgctggatat tgaaacgctg aataatgacg aacatgcgtg
    17161 ggatgcggta gtcgaagagt tcgatggtta tcggcaaatc tggcgcaaac gtggcgttat
    17221 gccgatgctg cgggcgctga tgtcggcgcg taacattgca gaaaacttgc tggcaacggc
    17281 aggcggtgag cggcgtctta ccgatatctt gcatatcagc gaactgcttc aagaagccgg
    17341 aacgcagctg gaaagtgaac atgcgctggt acgctggtta tcgcaacata tcctcgagcc
    17401 agacagtaat gcctccagcc aacaaatgcg cctcgaaagt gataaacatc tggtgcagat
    17461 tgtcacgatc cataaatcga aagggctgga atatccgctg gtctggttgc catttattac
    17521 caatttccgc gtccaggatc aggcgtttta tcacgatcgc cactcgtttg aggcagtttt
    17581 ggatcttaat gctgcgccag aaagcgtcga cctcgcggag gtcgaacgtc tggcggaaga
    17641 tctgcgtttg ctttacgtgg cgctgacgcg ttcggtttgg cattgcagtc tcggcgttgc
    17701 accgctggtg cgccgtcgtg gcgataaaaa aggtgacacc gacgtccacc aaagtgcgct
    17761 cgggcgtttg ctgcaaaaag gggaaccgca agatgcggca gggcttcgca cctgtattga
    17821 agcgttatgc gatgatgata ttgcctggca aacggcacaa actggtgata accagccctg
    17881 gcaggttaat gatgctttaa ctgcagaact gaatgcgagg acactacaac gattgcccgg
    17941 cgataactgg cgtgtcacca gctactccgg cttgcagcag cgtggtcacg gtatcgctca
    18001 ggatctgatg ccacggctgg atgttgatgc cgcaggcgtg gtcagcgtcg ttgaagaacc
    18061 gacgttaaca ccgcatcagt tcccgcgcgg tgcgtcaccg gggacattct tgcacagttt
    18121 gtttgaagac ctcgatttta cccagccggt tgacccgaac tgggtacagg aaaaactgga
    18181 actcggcggc tttgaatcgc agtgggaacc ggtgttgacc gagtggatca cggctgtcct
    18241 ccaggcacct ctcaatgaaa cgggtgttag cctgagtcag ctttccgacc gcgataaaca
    18301 ggtggagatg gagttttacc tgccgattag cgaaccgctc atcgccagcc agcttgatgc
    18361 actaattcgc cagtttgacc cgctatccgc tggctgcccg ccgctggagt tcatgcaggt
    18421 acgcggcatg ttaaaaggct ttatcgacct ggtattccgc cacgaagggc gttattacct
    18481 gctcgactat aaatccaact ggctgggtga agacagttcg gcttacaccc aacaggctat
    18541 ggcagcagcg atgcaggcac accgctatga tctgcaatat cagctttata ccctggcact
    18601 gcatcgttat ctgcgccatc gcattgctga ttacgactat gatcttcact ttggcggcgt
    18661 tatctatctg ttcctgcgtg gcgttgataa agaacatccg caacaaggga tttacacaac
    18721 ccgacccaac gccgggttga ttgccctgat ggatgagatg tttgccggta tgaccctgga
    18781 ggaggcgtaa tgaaattgca aaagcaatta ctggaagctg tggagcacaa acagctacgc
    18841 ccgctggatg tgcaatttgc cctgaccgtg gcgggagatg aacatcctgc tgtcaccctc
    18901 gcggcggcac tgttaagtca tgatgccgga gagggacacg tctgtttgcc gctttcacga
    18961 ctggaaaata acgaggcgtc gcatccgctg ttggcgacct gtgtcagtga aatcggtgag
    19021 ctacaaaatt gggaagaatg cttgctggct tctcaagcgg tcagcagggg agatgaaccc
    19081 acgccgatga tcctctgtgg cgatcgtctt tatttgaatc gcatgtggtg taacgagcgg
    19141 acggtagcgc gcttcttcaa tgaggtgaat cataccattg aggttgatga agctctactg
    19201 gcgcaaaccc tggacaaact ttttccagta agcgatgaaa ttaactggca aaaagttgca
    19261 gcggcagtgg cgctgacgcg gcggatctcg gtgatttccg gcggcccagg caccggtaaa
    19321 acgaccaccg tggcgaagtt gctggcagcg ttaattcaaa tggccgacgg cgaacgctgc
    19381 cgtatccgtc tggctgcacc aacgggtaaa gctgccgcgc gcttaaccga atctctcggc
    19441 aaggctttgc gacagttacc gctgaccgat gaacaaaaga aacgcattcc ggaagatgcc
    19501 agcactttgc accgattgct gggcgcgcag ccgggtagcc agcgtttacg tcatcatgcc
    19561 ggtaacccgc tgcatcttga tgtgctggtg gtagatgaag cgtcaatgat cgatctgcct
    19621 atgatgtcga gactgatcga cgccttgcca gatcatgcgc gagtgatctt tctcggcgat
    19681 cgtgatcaac tggcctcggt tgaggctggg gctgtgctgg gcgatatctg cgcttatgcc
    19741 aacgcgggct ttaccgccga gcgtgccagg cagctaagcc gcctgacggg gactcacgtt
    19801 ccggcaggaa ccggcacaga agcggcatct ttgcgcgaca gtctctgcct gctgcaaaaa
    19861 agctatcgtt tcggcagcga ttctggcatt ggtcagttag ctgcggcgat caaccgtggt
    19921 gataaaacgg cagtgaaaac cgtttttcag caggatttta ctgatatcga aaaacgactt
    19981 ttacagagcg gcgaagatta tattgcgatg cttgaggaag ctcttgcggg ttacggacgt
    20041 tatctggatc tgctgcaagc gcgtgccgag ccggatttaa tcattcaggc gttcaatgag
    20101 taccagcttt tgtgcgccct gcgggaaggg ccgtttggcg tggctggact gaatgagcga
    20161 attgagcagt ttatgcaaca gaagcgcaaa attcatcgtc atccgcactc tcgttggtac
    20221 gaaggccgac cggtgatgat tgcccgtaat gacagcgcgc ttgggttgtt taatggcgat
    20281 atcggtattg cgctggatcg cgggcagggg acgcgcgtct ggtttgcgat gccggacggc
    20341 aatattaagt ctgtgcaacc gagtcgcttg ccagagcacg aaactacgtg ggcgatgacg
    20401 gtacataaat cgcagggatc ggagttcgac catgcggcgt tgattttacc gagccaacgc
    20461 acgccggtag taacgcgaga gctggtatac accgcggtga cccgcgcgcg tcgtcgtctg
    20521 tcgctgtatg ccgatgagcg tatattaagt gcggcaatcg ccactcgtac tgagcggcgc
    20581 agtggtctgg cggcgttgtt tagttcgcgg gaataaacgt aattgccgga tgcgacgcac
    20641 gagtgttacg cctgccgcat ccggtgattt tcatcgctta ccctaaatcc gccatcaaca
    20701 ctttggattt acgctggtag ttgtacaact gctttttgct ctcgggcagt aaatcaatat
    20761 ccaccggggt aaatccacgt tcctggaacc agtgaatact gcgcgtggtc agcacaaaca
    20821 atttgcttaa gccgctctgc ttcgcctgag cggcaatgcg ttccagcaga acctcgcccc
    20881 gtgatgaact gcggtaatcc gggtgaactg ccacacaggc catttcccca atcttctctt
    20941 ccgggaacgg atagagcgcg gcgcaggcaa tagtcgtgtt atcgcgctga ataatggtga
    21001 atttgtcgat ttccatctcc agctgctcgc gagaacggcg caccagaata ccttgctgct
    21061 ccagcgggcg aatcaactcc agaataccgc caatatcgtt gattgttgcg cgacgaatct
    21121 gctcggcgct ttccatcaca atctgcgtac cgataccgtc gcgtgagaac aactcttgca
    21181 acagcgcgcc atcttcctga taactgatta aatgacagcg gcgcacgccg ctgcggcagg
    21241 ctttcactgc gccacgcaaa aagcgcaccg taccggagtt gtaatcgcct ttctcttcct
    21301 gggcttctac ccgtgcttgc gcttcgttag ggaaaagttc ggagacaata tcaccgtcgt
    21361 cattagtaac gccctgggaa gagcaaaaac caatcatctt ttcagctttc agtttgatgg
    21421 ctagttgagt ggcaatctct tcagaggtca gattaaagct ctcgccagtg actgaaacag
    21481 cgaccggccc cattagcact attgcaccgc tttccagttg acgatggatc gcgtcttcat
    21541 caatccgccg gatacgcccg ctatggcagt aatccacgcc gtcatcgacg cccagcggct
    21601 gggcaataat aaaattgcca ctaacgacgt tgatatgcgc gccctgcagc ggcgtgttat
    21661 tgagactcat tgacaggcga gcagtaatat ccagttgcaa tgttcccgca gcctgcttca
    21721 ccagttccag tgttttggcg tcggtcacac gtatattctt gtgatacagc ggttcgtggt
    21781 gatgcgcagc cagatttgcg tcgatctgcg gacgtgcgcc atagaccacc accagacgga
    21841 tgccgaggct gtgcaacaac ccgatatcat taacgatact ggagaaattc tcatgctcaa
    21901 tggcttcacc gccgagcatg atgacaaacg tttttccccg gtgggtattg atatagggaa
    21961 ccgaatggcg gaatccctcg accagctcgg ttttacgttc ctttaccacg gcacacccct
    22021 ttgcatgatt atttgaaatt attgtatttt tattctgttt tttcgcaggg tgcaagtgta
    22081 aattttatgc gagagcgact tttttatcag taatgccgtg aatataaaaa gaatgtttac
    22141 cgtttattag atgacagatt atgcgtcttt cgctaaagtt tccggtcaaa ttagtcgttt
    22201 acttgttaca cagcttagat ttcttttgct cgggagaggc atgtcaggat ccaacactgc
    22261 aatcagccgt cgtcgtttac tgcaaggcgc gggcgctatg tggctattga gcgtaagtca
    22321 ggtcagcctg gctgcggtta gccaggtcgt ggcggtgcgc gtctggcctg cgtccagcta
    22381 cacccgcgtg acggtagaat caaatcgtca gctgaaatat aagcagttcg cgttgagtaa
    22441 ccctgaacgc gtggtggtgg atatcgaaga tgtaaacctg aactcggtac tcaaggggat
    22501 ggcggcacaa attcgtgcag acgacccgtt catcaagtcg gcgcgcgtcg ggcaatttga
    22561 cccgcaaacc gtacgcatgg tttttgaatt aaagcaaaac gtaaaaccgc agctgtttgc
    22621 ccttgcgccg gtcgccgggt ttaaagagcg tctggtgatg gacctctatc cggccaatgc
    22681 acaggatatg caggacccgc tgctggcgct gctggaggat tacaacaaag gcgacctcga
    22741 aaagcaggtg ccgccagcgc aaagtggtcc acaaccgggt aaagcagggc gcgatcgtcc
    22801 gattgtcatt atgcttgacc ctggccacgg tggcgaagat tccggtgcgg tggggaaata
    22861 caaaacgcgc gaaaaagatg tggtattgca aatagctcgc cgtttgcgct ctctgatcga
    22921 gaaagagggc aatatgaagg tgtacatgac gcgcaatgaa gacatcttca ttccgttgca
    22981 agtgcgcgta gcaaaagccc agaaacagcg tgctgacctg tttgtctcta tccatgccga
    23041 cgcctttacc agtcgtcagc cgagcggttc ctcggtgttt gcgctctcaa ccaaaggcgc
    23101 aaccagtact gcggcaaaat atctggcaca aacccagaac gcctcggact tgattggtgg
    23161 cgtgagcaaa agcggtgacc gctatgtcga ccacaccatg ttcgatatgg tgcagtcgct
    23221 gactattgct gacagcctta agtttggtaa agcggtactg aataagctcg gaaaaatcaa
    23281 caagctgcat aaaaatcaag ttgaacaggc cgggtttgcc gtactaaagg caccagatat
    23341 tccctccatt ctggtcgaaa cggcgtttat cagtaacgtt gaggaagagc gtaaactgaa
    23401 aacggcgact ttccagcagg aagttgcgga gtctattctt gcggggatta aagcgtattt
    23461 tgccgatggg gcaacgctgg cgagaagggg atgataaaaa ggcgctgaat ggcgcttttt
    23521 ttatgggaga tcagaaacaa aaaaacaccc gttagggtgt tcaataattg gttgcggggg
    23581 ccggatttga accgacgacc ttcgggttat gagcccgacg agctaccagg ctgctccacc
    23641 ccgcgtcacc gtactgcttt tacttcatca aatttgattg gttgcggggg ccggatttga
    23701 accgacgacc ttcgggttat gagcccgacg agctaccagg ctgctccacc ccgcgtccgt
    23761 ggatgcgcac tatactccgc tcgcgttttc atgcaacctt ttttttaccc cgatcacgga
    23821 tttagcatga ttttgaacaa aaacaaacca ctaaagctta ttttttgtgc gaaatttgcg
    23881 tcagggagag gctgctcatt tcattaaacg cggcggtttg ttatcttcgt tgcgccttat
    23941 tttttaacct gaagaagaga acaatgaaag gacgttgggt aaagtacctt cttatgggca
    24001 cggttgtggc aatgcttgcc gcctgctctt ccaaaccaac cgatcgcgga cagcaatata
    24061 aagacgggaa atttacccag cctttctctc tggtgaacca gccagatgcc gttggcgcgc
    24121 cgattaacgc cggtgatttt gccgagcaaa ttaaccatat ccgtaattcg tcaccgcgtc
    24181 tgtatggcaa ccagagtaat gtttataacg cggtgcaaga gtggctgcgc gcaggcggtg
    24241 atacccgcaa tatgcgccag ttcggcattg atgcctggca gatggaaggt gccgacaact
    24301 atggtaacgt gcagtttaca ggctattaca cgccggtaat tcaggcgcgc catacccgcc
    24361 agggcgagtt ccagtatcct atttaccgta tgccgccaaa acgtggtcgt ctgccgtctc
    24421 gtgcggagat ctacgccggg gcattgggcg ataaatacat cctcgcttac agcaactctc
    24481 tgatggataa cttcattatg gatgtgcagg gtagtgggta tatcgacttt ggtgatggca
    24541 gtccgcttaa ctttttcagc tatgcaggga aaaacggtca tgcctatcgc agcattggta
    24601 aggtgctgat cgaccgtggc gaagtgaaaa aagaagatat gtcgatgcag gcgattcgtc
    24661 attggggcaa aacacacagt gaagccgagg ttcgcgagct gctggaacag aacccgtctt
    24721 tcgtcttctt taaaccgcaa tcttttgcac cggtgaaagg ggcaagtgcg gtgccgctgg
    24781 ttggccgtgc ttctgttgcc tccgatcgtt caattattcc gccaggtact accttgctgg
    24841 cagaagtgcc gctgctggat aataacggca aatttaacgg tcagtacgaa ttgcgtctga
    24901 tggtggcgtt ggatgtcggg ggcgcaatca aaggccaaca cttcgatatc tatcaaggga
    24961 tcgggccgga agccggacac cgcgcaggtt ggtacaacca ctatggacgg gtttgggtgc
    25021 tgaaaacggc tccgggcgca ggtaacgtct ttagcggctg atgtgatatt ctgtatgccg
    25081 tttttacgga tatgacaggg tgaggggtaa cctcacatcc tgattattga ctaagtgcgc
    25141 gttgttcata ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcat gcaaatccaa
    25201 aatattgcag gagttatgta ggcctgataa gcgtagcgca tcaggcattt atgcgtttag
    25261 ggtgagggat aacctcgccc tttttaattc tgaggtgcta tgtctgtggt aattagtgat
    25321 gcatggcgtc agcgttttgg tggcacagcg cgtctgtatg gtgaaaaagc gttgcaactg
    25381 tttgctgacg cgcatatttg tgtggttggt atcggtggtg tcggttcctg ggcggcggaa
    25441 gcgctggcgc gcacggggat tggcgcaatc acgcttatcg atatggatga tgtgtgcgtc
    25501 accaatacca atcggcaaat tcacgctctg cgcgataacg tcgggctggc aaaagcggaa
    25561 gtcatggcgg agcgcattcg ccagattaac ccggagtgcc gtgtaacggt ggtggatgat
    25621 ttcgtgacgc cggataacgt agcgcaatat atgagtgccg gttattcgta cgtgattgat
    25681 gccattgata gtgtacggcc caaagcggcg ctgattgctt attgtcggcg caataaaatc
    25741 ccgctggtca cgaccggtgg cgcgggtggg cagattgacc cgacgcagat tcaggttacc
    25801 gatctggcga aaacgattca ggatccgctg gcggcgaagt tgcgcgagcg cctgaaaagc
    25861 gattttggcg tagtgaaaaa cagtaaaggt aagctcggcg tggattgcgt gttttctact
    25921 gaagcgctgg tgtacccgca gtcagacggt acggtgtgtg cgatgaaagc cacggcagaa
    25981 gggccgaagc ggatggattg tgcatctgga tttggcgcgg caacgatggt gaccgccacc
    26041 tttgggtttg ttgcggtttc tcatgcgctg aagaagatga tggcgaaagc ggcgcgtcag
    26101 ggttaaacaa tcaggtcgga tgagacgcga acgtcttatc cgacccggtt ctgtgcttaa
    26161 acctgcttcg tcgcagcgat aatcgcctcg cttaacgcat ttaacccctg gctgcgtgag
    26221 gcgctaagct gcgcacgtaa tcccagctca tcaaacaatg ccagcggcga ctgtgcctgt
    26281 aattcggcgg cggttttccc ctcaacggca gtcaacaaca ccgccaacag gccgcgcaca
    26341 atgcgccctt cgctgtcgcc aaagaagtgc attttgccgt tttcagccac ggtatatccc
    26401 agccagacgc ggttttcgca tccggcaatc tctttagcct gcgcttttaa ctcgtctggc
    26461 aaagccggaa gctgtttccc cagcatgatc aactggcgat atttatcttc ccattgcgac
    26521 aacggtgcga aggtattgcg taacgtttct gcggttacgg ttgtgccgaa cggatgtccg
    26581 gcgaattgcg ggtttgtcat taatccacca ataattccag cgcgcggtca acggcattca
    26641 ccagcgcatc cacatcactc tttgtattat atggcgcaaa tgaggcgcgc agtgtgccgg
    26701 ttacgcctaa ttctgccagt agcggctgag cgcaatgttg cccggcccgc aaggcaatac
    26761 cgtactccgc cagtagcgtc accatatcgc tgtgatgaac gccagcaaaa tcaaaggcca
    26821 gcaggctgga atcctggcag cggaatgaac gaaagcctgg acgtttcgcc agcgcttctt
    26881 ctgcaagcgt tgctaagcta cggctccagc tttcggcctg gttgatatcg taatctgcca
    26941 gccattccag cgccgcgctt aatcctatga cgccagcgac atttggcgtt cctgcttcca
    27001 gtttccacgg cgcagattga gtggtgaaac cgtcaaaact cacttcgtga atcattttgc
    27061 cgccacccag ccagggcgac atcgcttcca gcagttctga tttgccatac agcacgccga
    27121 tacccgtcgg gccatacagt ttgtgaccag agaaagcata gaaatcgata tccagttgct
    27181 gaacatccgc ggggaaatgc accgccccct gagcaccatc aaccatcacc accatcccgg
    27241 ctgaatgagc aaaggtaatc gctcgcgcca gatccgggca accgccagtc acgttcgaca
    27301 tctgacccaa cgccagaatc cgactacggg gagtaatcag ttctggcaac aaatcgacat
    27361 ccggcagtcg ctgcgcatta agcggcaatt tcaccacttt ggctctagtt tgttgggcga
    27421 ccatcagcca ggggacgagg ttagcgtggt gttctgccac gctgacaata atctcatcgc
    27481 ccggttgcag acgcggacgc gcatagcatt gtgccaccat gttgatggat tcagtggtgc
    27541 cgcgcgtcca gacgatagtt ttatcatccg gtgcattcag taattgcgcc actttctctc
    27601 gtgcagcttc ataacgcgcg gtcaggcgtt gggcttcggc aaactggctg cgatggacgt
    27661 ttccggcgct caggctgtaa aactgtcgag tggcttcaac cacggcttca ggtttaagcg
    27721 cggtcgcggc gctgtcgaga tagacgcccg catcctgtag tgcgggaaac tgggcgcgaa
    27781 actgcgcggg attaaaaacg ttcatggtac tcctcggctt gatgcaccga tcgtgacgca
    27841 aaattcactt tgatacaagg agagtgctaa ccatcagaat tatctagata tcctggcgaa
    27901 gccgcgtaag taacatatgc tgaatagtgt taggcgttaa ttttagcctg ttaatcaata
    27961 ctattaagat ttattaatcg ctttaaggag aagatgatga aaaagactgc cgcaattatt
    28021 tctgcctgta tgctgacttt tgccctgagc gcctgttccg gttcgaacta tgtgatgcac
    28081 accaatgacg gacgtaccat cgtctctgac ggcaaaccac agactgataa cgataccggt
    28141 atgatttcgt ataaagacgc taatggcaac aaacagcaga tcaaccgtac tgacgtgaaa
    28201 gagatggtcg aactggaaca gtaattcgcg atcgcaaggt aaaaaaaagc accgcaatta
    28261 ggcggtgcta cattaatcac tatggacaga cagggtaaat gtacaggaag tgaaaaaagg
    28321 tagctttgct accatggtct gaatcgcaga ccaattgcaa acacaacaac acaacatcac
    28381 aaccgtaagc caaaagttca ccagaatacg cattccgata aaacttttcg ttccggctca
    28441 ggaagtgccg ccactatagg tatttgctgg tagaagctca acggacaatt tataatggct
    28501 cagattaaaa aaactaatag gttacacagt gtgatctaat tgttaaattc atttaacatc
    28561 aaagtttaaa agccatgtct aaacgattac caccgctaaa tgccttacga gtttttgatg
    28621 ccgcagcacg ccatttaagt tttactcgcg cagcagaaga gctttttgtg acccaggccg
    28681 cagtaagtca tcaaatcaag tctcttgagg attttttggg gctaaaactg ttccgccgcc
    28741 gtaatcgttc actcctgctg accgaggaag ggcaaagcta tttcctcgat atcaaagaga
    28801 tattttcgca attaaccgaa gcgacgcgta aactccaggc ccgtagcgcc aagggggcgt
    28861 tgacggtcag tttactcccc agtttcgcca ttcattggtt ggttccgcga ctttccagct
    28921 ttaattcagc ttatccggga attgacgttc gaatccaggc ggttgatcgt caggaagata
    28981 agctggcgga tgatgttgat gtggcgatat tttatggtcg gggcaactgg ccggggctac
    29041 gggtggaaaa actgtacgcc gaatatttat tgccggtgtg ttcgccgcta ctgctgacag
    29101 gcgaaaaacc cttgaagacc ccggaagatc tggctaaaca tacgttatta catgatgcgt
    29161 cacgccgtga ctggcagaca tatacccgac agttggggtt aaatcatatc aacgttcagc
    29221 aagggccaat ttttagtcat agcgccatgg tgctgcaagc ggctattcac gggcagggag
    29281 tggcgctggc aaataacgtg atggcgcaat ctgaaatcga ggccggacgt cttgtttgcc
    29341 cgtttaatga tgttctggtc agtaaaaatg ctttttatct ggtttgtcat gacagccagg
    29401 cagaactggg taaaatagcc gcctttcgcc aatggatcct ggcgaaagcc gctgctgaac
    29461 aagaaaaatt ccgctttcgt tatgaacaat aatttacgta gggtacgacc atgaccagcc
    29521 gttttatgct gattttcgcc gccattagcg gcttcatttt tgtggcactg ggcgcttttg
    29581 gtgcgcatgt gttaagtaaa accatggggg ccgttgagat gggctggatc caaaccggtc
    29641 tcgaatacca ggcgtttcat acgctggcga tcttaggtct ggcggtggca atgcagcgtc
    29701 gcatcagtat ctggttttac tggagtagcg ttttcctcgc gttaggcacg gtgctgttca
    29761 gcggcagcct ttattgcctg gcgctgtccc atctgcgttt gtgggcgttt gtcacaccgg
    29821 ttggcggcgt gagcttcctt gcgggctggg cgttaatgtt agttggtgct atccgtttaa
    29881 agcgcaaggg cgtaagtcat gaataaggtt gtattgctgt gccgtccggg ctttgaaaaa
    29941 gagtgcgccg cagaaattac cgataaagcc ggccagcggg aaattttcgg ttttgcccgc
    30001 gtgaaagaga atgcgggtta tgtcatttat gaatgttatc aacctgatga tggcgataag
    30061 ttaatccgtg agctgccgtt cagttcatta atttttgccc gccagtggtt tgtggtgggg
    30121 gaactcctgc agcatttgcc gccagaagat cgtattaccc ccattgtcgg catgttacag
    30181 ggtgtagtag agaagggcgg tgaactgcgt gttgaagttg ccgataccaa cgaaagcaaa
    30241 gagttgctga aattctgccg taagttcact gttccgctac gcgctgcctt gcgcgatgct
    30301 ggggtgctgg cgaactatga aacgccgaag cgtccggttg tgcatgtatt ctttattgca
    30361 ccaggatgct gctataccgg ttactcatac agcaacaata attcgccgtt ctatatgggc
    30421 attccgcgcc tgaaatttcc ggcagaggcg ccgagtcgtt ccacgctcaa actggaagag
    30481 gcatttcatg tgtttattcc tgcggatgag tgggatgaac gcctggcgaa cgggatgtgg
    30541 gcggtggatt taggcgcttg ccctggcggc tggacctacc aactggtgaa gcgcaacatg
    30601 tgggtttatt ccgtcgacaa cggcccgatg gcgcaaagtc tgatggatac cgggcaggtg
    30661 acgtggctgc gggaagacgg tttcaaattc cgtccgacac gcagcaatat ctcctggatg
    30721 gtatgcgata tggttgaaaa accggctaaa gttgcggcat tgatggcgca gtggctggtt
    30781 aatggctggt gccgtgaaac cattttcaat ctcaaactgc cgatgaaaaa acgctacgaa
    30841 gaagtatcac acaatctggc gtatattcag gcacagcttg atgaacatgg cataaatgct
    30901 cagattcagg cgcggcagtt gtatcacgat cgcgaagaag tgacggtgca cgtccgccgc
    30961 atctgggctg cggtgggtgg tcgtcgcgac gagcgataac aggcaaggcc ggggcgtcgt
    31021 ctccggcctg ctaccctttc cgtcaggctg ttaccaaaga agttgcaacc tggcgctcat
    31081 cagaaataat gtgcgttacc tcatccagat acccgatgcg ggcttcccct gaacgattaa
    31141 atttactttt atcaatcaat aacaacgatt gcgcggcacg ttttaatagc atcgatttgt
    31201 aatcagcgtt gatcgcattg gagtcccaca gtgcgccgct gctatcgatc ccttcacaag
    31261 aaaaaataaa cagatcgatt tccagcgatt taagttggga aatcagcgag ggattgacgt
    31321 aacagccata tttgcgctca agtgtgccgc cggaactgat cagttgaatg cgttcgcgtt
    31381 taccgagttc atggcaaatc ggatggctat tggtgaagac ctgaatgttg atgtcaggca
    31441 actggcgtgc cagataccag caagttgaac tggcgtctaa ggctatcacc atcctttctt
    31501 caatccacgc gagcgcctcg cgcgcgatat ctgctttatg cgcataatgg cttttcagcc
    31561 tgatgtgaaa gagagcgcta ctgtcttgat tttgacggtg gatatattta gcgcgtccat
    31621 gattgcgcag aattttaccc tgcgtctgta attcattgag atcgcgacga atggtttctt
    31681 tactgacctt tagctgttca gagagagctt ccgtggtcag gctggtatgg ttcagcagca
    31741 ggtcgactat cgcttgctgg cgtgccgctt tcatattttt caccccatca ttcgataacc
    31801 gcccgcgtca tgcgggcgta ccggtatgag attacggtgt tacccctttt tttaaaagaa
    31861 tattcccgta cttcgctcgt tcgcctgtga taacgatcgc aaaggctttt tgcgcccgtt
    31921 cataaaacgc aaaacgattg atgcggatga tgtcaggaca cggggcttgt agtgaaagcg
    31981 cattacggta acgtcgttct acttcaggat cgagagtgtc accctctacc gccgccatca
    32041 tcaccagcgg cggggcataa ctgtccagct caaataacgg gataatcgcc tggagcaagt
    32101 cgctcaccaa caggccatca gcgcggatca cctgcggtcc catcgaatgg gcgggaaagt
    32161 gagcatcgga aaaaataatt tcatctccat gtcccatctc tgccagcact ttcaatagtt
    32221 cgggagaaat taacggcgaa attgttttca gcatttcaca cttcctctat aaattcaggt
    32281 tcagtttgcg ggtagaaata acggtactga taatgaatct gtgcacgggc ttcttccggg
    32341 ctgttaaatt cccctacgcc ataccaaccg aacagcgcag ctcctgcgac ggtcgtttcg
    32401 gcatcgtcga gaacttttac cggaatatca agcatattgg ctttaatctg attccacaat
    32461 gtgttgcgac ttcctccacc gactaacaat aattcatagg ctttaaagtg cccgattttt
    32521 tccagcatct gtagattgcg ctgtaattgc gcagttaacc cttccagcgc cgcgcgatag
    32581 aaatgccccc gcgtggtatt aagcgtcact ccttgccagc cagcgttctg acacgacaat
    32641 aaatcacact gcattttcac accatccgcg ccaggtgcga tcagacgagc ttcttcaatc
    32701 aacatttgcc agggtgtttc agccgtccag aacagttttc tcacccattc cagcacgccg
    32761 gatgccagcc attgcatacc tgggttatac aaccatgcct ggctatctag ttcgcaggtg
    32821 gaaccggcgt actgacttaa cagcgaagta tcaacctagg cgctgcgaac cattaaaatt
    32881 tcccatgtac cggaggagag cacgggttca ttttgttcgg caccagcgcc aaaaagggcg
    32941 aactgggtat cgtgacctgc ggaaatcacc ggtatgccaa cgggtaagcc gagcattgct
    33001 gcggcgctgt tctgtagcgt accaatctgt tcacccgctt ccaccagacg agggaagagt
    33061 cggcgtggaa taccggtggc ttgtaaaatt tgcggactga aatcgcgttg ctggatatcc
    33121 agcatctggc tggttccggc tatcgtgata tcagtagtga attcgccggt taaacggtgg
    33181 ttaatcagcg acgaaatgaa gagccaggcg tgcgcgcgtt ccagcagttg tggatgattt
    33241 tctttcaacc acaccaactt atataacgta ttgaaactaa aggctccgac gccagaaata
    33301 gcctgcaacc gctgtgcgga gattaaccgt tcaatattgt ccataaccgc tgctgttcgc
    33361 ggacatttcc agctaatgat cggatagagc agattgcctt gcttatctac cagagcgcca
    33421 tccacaccaa aggtggtgac ggcgataccg cggatgtggc attcagtcag ttcactattg
    33481 atttgccgac agcaatcagc aaagcgttgc aaaatggcag tctaaagacc actggtgcca
    33541 ggtgttgttt tccatcgcga tatcgctggc attaggcgtt gaggcgcggg caacaatttt
    33601 tccctgccga ttaaccgcga tggccctgac attggtcgcg ccacagtcga gtaccaggat
    33661 aacttcttgt ttcataatcg ctcctgcaat atagccggat aacattgttt atccggctaa
    33721 ccactcttgc ttgttcaggc ccggtagccg gagcgaccgg gcatcacatc agggtgtaat
    33781 gtattaacgc ttgtacaacg gaccgtagtt ctggcaagcg cggtaatcct ggccttcaat
    33841 atccatgccg tgcgcagccc aggcagaagg acgatacact ttggtctctt caacgttgtg
    33901 catacatacc gggatacgca gcatggaggc aagggtgata aagtctgcgc caacgtggcc
    33961 gatggtcaga actccgtggt tggcacccca gttcgccatt accgaataca catccgtaaa
    34021 cggcccttta ccagtgaggc gcggtgcaaa ccaggtggtt ggccaggttg agttggtgcg
    34081 tttgttgagg atgtcatgca catccttcgg caattccacg ctccagcctt ccgcgatttg
    34141 cagtaccggt cccaggcctt tgatgatgtt gacacgagtc atggtgaacg ggacgccgcc
    34201 ttcggtaagg aagcgggaag agtaaccgcc gccacggaag tattcgtgga tcgccgggca
    34261 ccattcggta gcggcgaggc aagcgtcagc ctcttgctga gagatttccc agtgcggctt
    34321 catcgtcggg ttaccttcgc tgtcgcgttg tttacaggaa ccgtccagcg cagcagaacc
    34381 tgagttgatc aaatggatga tgccgtgttc tgccagtccg tccagtttat gccccgttac
    34441 acgctcaatt gcttctggtg accagtaggt acgcacatcg gcaaatacct gagcggtgcc
    34501 ggtgagctgg tgacccatta gcattgccac gccgttaaga ctgtcgtttt cggtcgccac
    34561 gacaaagggt tcgcgcacgc cattccagtc aaatgaactg ttgaggatcg cttcggcggt
    34621 gtcaccattg ggatattgat cggtccagtg acgttgcccc tggaagcccg cagcgatggc
    34681 gttgtagcca agtgattctt ccacgcgacc aatatcggcc agtttgctgt tgccttgcat
    34741 catgtcgcgg atacacatcg ccatcagtaa actttcgcgc agaactgcgc ggctttgctc
    34801 ggcattgcgt tgatactgtt tgttattttc atcttcgcca tagcggaagt ttttatcagc
    34861 ccaggccagt gccatttcca gttcggcttc gtcgtaaatc ttctgatcga tacggcgacg
    34921 cagttcggtc atatccaccg cctggacttt cattcccagc caggattcaa agaagttgtg
    34981 atcaacaata gaaccggcga tacccatcga aacgccgccc agcgacagat agcttttacc
    35041 tttcatgctg gcgacggcca aaccggcgcg ggcaaagcgc agcagttttt cttcaacatc
    35101 ggcaggaatc gatgtgtcat cggcatcctg aacgtcatga ccgtaaatgg agaatgccgg
    35161 gatgcctttc tggctgtgag ctgccagagc cgctgccagg taaacagcgc cggggcgttc
    35221 agtgccgtta aagccccaaa tggctttcgg gcgggttgga tccatgtcga tggtttcact
    35281 gccatagcac cagcaaggcg ttaccgtaat ggtgaggcct acattctggc tgctgaattt
    35341 ttcttcgcaa gcagcggctt cagccatacc cgcgatacag gtatcggaaa tgacacactc
    35401 gacggcagct ccgcaggtat ggcgcagttt ctcggtcagc agtgcggccg tagctttcgc
    35461 catattcatt gtttgttctt caagcgactc acgaacaccc atgcgacgac cgtcaataac
    35521 cgggcggata ccaattttcg gtaagctgat ttttttcatt acagattcct cactttattc
    35581 ggaaaatatg ttcagttagt tgccgtttga gaacggaaac gggcaaagat aaagatgacc
    35641 gcgaagcaga gtgcggggat cagttcagca gtggggatgt tgcccgccgc gtcactgaca
    35701 aaacccatga ccggagtgac aataccgccg ccaataatgg tcataacgat gaaggacgaa
    35761 ccgtatttgg tgtcctggcc gagattctta atgcccagcg agaagattgt tgggtactga
    35821 atcgacataa aggcgctgca taaagtcagg gctattaagc ccacatgacc gccagcgaag
    35881 gctgagatca ggcacagtgc catagcgatt aatgcgtagg cggccaggac tttgtgtggt
    35941 gcgaagcgac tgatgagcca ggtaccggtg atacgaccaa taaagaagca caccatggtt
    36001 ccggttaaat agttagcggc aaagcctgca gtcatacctg gaatttcttc tacagcgtag
    36061 cgaatcaaat agctccagca ggccgtttgt gcgccgacat agcagaattg cgctaatacc
    36121 gcccagcgcc agtgacgaat acgcgccaga cgagaaagcg atgcggagaa cgatccttgt
    36181 ttggcgtcac tgtgattatc actctgcaat g
//
LOCUS       DAJVQH010000024        35637 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJVQH010000024 DAJVQH010000000
VERSION     DAJVQH010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.024"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..218)
                     /locus_tag="OQM87_001323"
     CDS             complement(<1..218)
                     /locus_tag="OQM87_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1507523.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAEVY"
     gene            complement(219..305)
                     /locus_tag="OQM87_001324"
                     /pseudo
     CDS             complement(219..305)
                     /locus_tag="OQM87_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(399..1874)
                     /locus_tag="OQM87_001325"
     CDS             complement(399..1874)
                     /locus_tag="OQM87_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS1507524.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(2021..3305)
                     /locus_tag="OQM87_001326"
                     /pseudo
     CDS             complement(2021..3305)
                     /locus_tag="OQM87_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
     gene            complement(3418..5352)
                     /gene="yeaG"
                     /locus_tag="OQM87_001327"
     CDS             complement(3418..5352)
                     /gene="yeaG"
                     /locus_tag="OQM87_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCS1507525.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGTLCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILPRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5788..6534
                     /gene="mipA"
                     /locus_tag="OQM87_001328"
     CDS             5788..6534
                     /gene="mipA"
                     /locus_tag="OQM87_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCS1507526.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6624..7385
                     /locus_tag="OQM87_001329"
     CDS             6624..7385
                     /locus_tag="OQM87_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCS1507527.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7436..8320)
                     /locus_tag="OQM87_001330"
     CDS             complement(7436..8320)
                     /locus_tag="OQM87_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCS1507528.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8404..9399)
                     /gene="gapA"
                     /locus_tag="OQM87_001331"
     CDS             complement(8404..9399)
                     /gene="gapA"
                     /locus_tag="OQM87_001331"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1507529.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9741..10154
                     /gene="msrB"
                     /locus_tag="OQM87_001332"
     CDS             9741..10154
                     /gene="msrB"
                     /locus_tag="OQM87_001332"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCS1507530.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            10196..10468
                     /locus_tag="OQM87_001333"
     CDS             10196..10468
                     /locus_tag="OQM87_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCS1507531.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10838..11914
                     /locus_tag="OQM87_001334"
     CDS             10838..11914
                     /locus_tag="OQM87_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1507532.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11941..13320
                     /locus_tag="OQM87_001335"
     CDS             11941..13320
                     /locus_tag="OQM87_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1507533.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     SLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            13337..14382
                     /locus_tag="OQM87_001336"
                     /pseudo
     CDS             13337..14382
                     /locus_tag="OQM87_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14403..15239
                     /locus_tag="OQM87_001337"
     CDS             14403..15239
                     /locus_tag="OQM87_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCS1507534.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            15244..16192
                     /locus_tag="OQM87_001338"
                     /pseudo
     CDS             15244..16192
                     /locus_tag="OQM87_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            16202..17182
                     /gene="ydjG"
                     /locus_tag="OQM87_001339"
     CDS             16202..17182
                     /gene="ydjG"
                     /locus_tag="OQM87_001339"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCS1507535.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            17319..18077
                     /locus_tag="OQM87_001340"
     CDS             17319..18077
                     /locus_tag="OQM87_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1507536.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            18195..19553
                     /locus_tag="OQM87_001341"
     CDS             18195..19553
                     /locus_tag="OQM87_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1507537.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19646..20287)
                     /gene="pncA"
                     /locus_tag="OQM87_001342"
     CDS             complement(19646..20287)
                     /gene="pncA"
                     /locus_tag="OQM87_001342"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCS1507538.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(20298..21314)
                     /gene="ansA"
                     /locus_tag="OQM87_001343"
     CDS             complement(20298..21314)
                     /gene="ansA"
                     /locus_tag="OQM87_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCS1507539.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TLDD"
     gene            complement(21481..23337)
                     /gene="sppA"
                     /locus_tag="OQM87_001344"
     CDS             complement(21481..23337)
                     /gene="sppA"
                     /locus_tag="OQM87_001344"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCS1507540.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23498..24049
                     /locus_tag="OQM87_001345"
     CDS             23498..24049
                     /locus_tag="OQM87_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCS1507541.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            24166..25209
                     /gene="selD"
                     /locus_tag="OQM87_001346"
     CDS             24166..25209
                     /gene="selD"
                     /locus_tag="OQM87_001346"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCS1507542.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            25214..27175
                     /gene="topB"
                     /locus_tag="OQM87_001347"
     CDS             25214..27175
                     /gene="topB"
                     /locus_tag="OQM87_001347"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCS1507543.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            27302..28342
                     /locus_tag="OQM87_001348"
     CDS             27302..28342
                     /locus_tag="OQM87_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1507544.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQLGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28459..29802)
                     /gene="gdhA"
                     /locus_tag="OQM87_001349"
     CDS             complement(28459..29802)
                     /gene="gdhA"
                     /locus_tag="OQM87_001349"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCS1507545.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            30038..30310
                     /locus_tag="OQM87_001350"
     CDS             30038..30310
                     /locus_tag="OQM87_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCS1507546.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(30276..30683)
                     /gene="nudG"
                     /locus_tag="OQM87_001351"
     CDS             complement(30276..30683)
                     /gene="nudG"
                     /locus_tag="OQM87_001351"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCS1507547.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            30770..31426
                     /locus_tag="OQM87_001352"
     CDS             30770..31426
                     /locus_tag="OQM87_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1507548.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ
                     "
     gene            complement(31435..32742)
                     /gene="ynjE"
                     /locus_tag="OQM87_001353"
     CDS             complement(31435..32742)
                     /gene="ynjE"
                     /locus_tag="OQM87_001353"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCS1507549.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGGWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(32809..33462)
                     /locus_tag="OQM87_001354"
     CDS             complement(32809..33462)
                     /locus_tag="OQM87_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1507550.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(33462..34996)
                     /locus_tag="OQM87_001355"
                     /pseudo
     CDS             complement(33462..34996)
                     /locus_tag="OQM87_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34969..>35637)
                     /locus_tag="OQM87_001356"
     CDS             complement(34969..>35637)
                     /locus_tag="OQM87_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1507551.1"
                     /translation="QTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAA
                     LKEAPDDATFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFN
                     PAHAQQKIASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQ
                     LRKADPVVWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRR
                     YGTH"
BASE COUNT         9049 a         8428 c         9114 g         9046 t
ORIGIN      
        1 cagtacacct cagcgagagc gcgcatcaca tgcgcggcaa tactctgatt ctgatactta
       61 tcataaagaa aggaggagtc tgctttctgc acgatatagc gcagataggc actcattgct
      121 ctgtcgctgg cgacaagttc aaaaataagg aaggaagatg tcagcacaat gacacttgcg
      181 gcgataaaat gccggagcat tctatggtgc aatttcatga taaatggcac tccttcggtt
      241 agtgaaacga gaaaaccaga taaagttcga ttgcggcctg gcacaggggc gctaattata
      301 ctcatctctg cgccgctggg gaggggaaat ttaggggtaa tagtaataaa ttgtatgcaa
      361 aagccacgca aaaagcatgg ctctgaatat ttttgcactt aaatattatg aataatcact
      421 ttgttgcccc cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt catgacaagc
      481 gagatattac gcgtgtcacc cgttgaaaaa aacgtgccaa tactgatggt catttgttca
      541 ggaacatcat atctgttact gaagccggtc aggagttcga catttttacg catacgttcc
      601 gcaatgattt ttgcctgggc actattgagt tcggtaaata gcaagccaaa cacctcaccc
      661 tccagtcgcg ctaaaatatc atcggggcgg atgctttgct ggataatatc gacaattgaa
      721 accagcactt tatcacccac acgatgtccc cattgggcat tgatgcgttt gaactgatta
      781 atatcaacca gcatgacaca gaagtcctta acaacaggtc gcgacaataa tgactctacg
      841 ctgttgaaaa agtagcgccg attataaata ttggtcagca catcatgaac tgccagtttg
      901 ctggagagtt gcagttcctg aaaaatgtta taaatcagaa aagacacaac aaacagttta
      961 ctggagactt caatcccgcg actgatgtac cagatggtgt aattatactc atccagagtc
     1021 agtagaagta agttgcagca tagcgatgcc agacataata atgctattaa tggccatagt
     1081 tcgctggcta atcgtgtacg catgataata aagaacagta aaaatgccca cagacaaacc
     1141 aatatttttg tatagttgat tccccaggtc gcagtatggt tgtcaggaca gtaatcgacg
     1201 acatacaaag aatagtcagc gttataactg ctcagattgt gtgccagaag gggaaaaact
     1261 aaaaaaggaa tcagcgccag caacagaatg ccagtttttt tcttattgtt gtcaaggatg
     1321 ttgtctttac cataacaaaa gagcgccaac gaggttaaac aaataaaact gagttggcgg
     1381 aaaatataga aaatagaaac atcattcgac tttgtctgga tcagtgtact gtcgctgata
     1441 ggtttatgga taacaacaat agtttcaact aaatagataa ggccacttaa aagtgcaaga
     1501 cttaaaatga gtgttggtag aatttgtttg ttagtgaaat atttcattaa catgaaaaga
     1561 aaaatgattg cgttaaggaa aaacaaagcc acaacgacca tcagataatt catttgtggg
     1621 atgagtttta cattcgtctt ttcgttatat aatgtggtga tgcaaaataa aattaacgtc
     1681 agcaataaac tgaaaataaa aaagctttga gcttttaatt tttgtgtgcc catagaaacc
     1741 ataaaaagga gagcaatgtg agtgctggat tttattgatg gcagagatat atagttttga
     1801 ccaagatcaa ttttcgttaa cgataaaaaa tatttaaaga ataaccccag ggaaatacgt
     1861 gttgactgaa tcatttttcc gaatgattgt tttctggaac taaattgtgt aaaggttatg
     1921 ctgtcatatt taaagcgatt gtaagctaat gtatgtaata aatgagataa tttacagtaa
     1981 gctaaaaatc agccaggcga taatgacccg gctgatagtt ttagtcttta gctgttgcat
     2041 tttgtttatg aaacagttca cggaacaccg gataaatatc atcctggtcg cggatgtgct
     2101 gcatcgcaaa gttgtcgaaa gtagattgca gatgctcata ttctcgcaca caatgtctga
     2161 tgtgcacgac gggtaatttc gatatagctg taataacgaa caacaggtaa taattttttc
     2221 gccaggattt catggcaaag cggagagtca tcggcccagt tatcgccgtc cgatgcttgt
     2281 gcagcgtaaa tattccactg tgccgggtta taacgctctt tcactacctc atccatcagt
     2341 ttcagggcgc tggaaacaat ggtgccgcct gtttcctgcg agtagaaaaa ctcatgttca
     2401 tcgacttctt tcgcctgggt atgatggcgg atgtatacga cttccacgtt cttatacgtt
     2461 ctgctgagga acagatacag cagaatataa aaacgcttag ccatatcttt agtggattga
     2521 tccattgaac cggaaacgtc catcaggcaa aacatcactg cctggctgga gggatcgggc
     2581 cgcttctcgt agttcttgta acgtaaatcg aaggtgtcaa taaaagggac gcgttcaatt
     2641 ttggcacgta attctgcaat ttctttgcgc agacgttcct cttccagcag ctgcgcaggt
     2701 tcactgttgc tgatgatggc caaattctct tccagtgcat gaagttcccg ccgcttgcct
     2761 gccgtcatgg ctgtgcgtcg cgccagtgag ttctgcaagg aacgcacaac gctgatattg
     2821 gctggaacgc cgttagcggt ataacccgcc cgatgcgttt tatattcggt cagctggcgt
     2881 tgttggtttt gtttcagatt cggtaaggcc aaatcttcaa agagcagatc aagatactca
     2941 tctttcgaaa tctgaaagac aaattcatcc tgaccttcac catcctggct ggcctggccc
     3001 tgaccactgc cggaacctcc gccgccaccc tggggacgtt caattcggtc gttctgaacg
     3061 aaatggtcat tgcccggatg cacgcggtgg cgcagaccgc cacgcccctg atgaaacatt
     3121 ggttcgctaa tatcttccgt gggaatggat acggattcgc tgctgtcgac gtcagtcacc
     3181 gaacgcttat taatggcctc ggagatcgac tgtttaattt gcgctttata acggcgtaaa
     3241 aaacgctggc gattcaccat gcttttgttt ttgccgttca gacgccggtc aataaaccag
     3301 gtcatatgcc ccccgtacta catttgccaa ctgtatgcta cgcctgtgtt gccgcttctc
     3361 aggcccacgg ttcgctatcg ttgtaggccc ggtagcatgt gtaccgggcc agggttgtta
     3421 agacgattta cgtacgcgca aataccattc gcacagtaaa cgcacctgtt tacgggtgta
     3481 gcctttttcc atcatacggt cgacaaagtc gtcgtgtttc ttctgctcgt cggttgacgt
     3541 tttggcgtta aacgagataa ccggcaacag ctcctcggta ttggagaaca ttttcttctc
     3601 gataaccgtg cgcagttttt catagctggt ccagttcgga ttgcgtccgc tgttattcgc
     3661 tctggcgcgc agtacgaagt taacaatctc gttgcggaaa tcttttggat tactgatccc
     3721 cgccggtttc tcgattttct ccagctcggc gttaagagac tcgcggtcaa acagctgccc
     3781 ggtatccgga tcgcgatact cctgatcctg aatccagaaa tccgcgtagg taacataacg
     3841 gtcgaaaatg ttttgcccat attcggaata ggattcaagg taggccgtct ggatctcttt
     3901 gccgataaac tcggcatatt tcgggatcag ataacctttc aggaactcca gatagcgttc
     3961 tgcctgctct tgcgggaact gctcgcgctc gatctgctgt tccaggacgt agaacagatg
     4021 gaccgggttt gccgccactt ctacatgatc gaagttgaac acgcggggga ggatcttaaa
     4081 cgcaaaacgc gtcgacagac cgttcatccc ttcatcgaca ccggcgtagt cacgatattc
     4141 ctgatacgac ttggctttcg gatcggtgtc tttcagactt tcgccatcat aaacccgcat
     4201 ctttgaataa atgctggagt tttctggctc tttcaggcgc gaaagaatgg aaaaacgtga
     4261 cagtgtttcg agcgtgccag gggcgcatgg ggcgtgagtc aattcactgt gattaagcaa
     4321 tttctcgtag attttgatct cttcggaaat gcgcaagcaa tacggcacct tcacgatgta
     4381 aacacggtcg aggaaggctt cgttgttttt gttattacgg aaagtgaccc attcggactc
     4441 gttcgagtgg gcgagaataa tcccgttgaa cggcagggcg gagatacctt ccgtcccgtt
     4501 gtagttatct tcctgagtgg cggttaacaa gggatgcagc acttttatcg gtgctttaaa
     4561 catctcaacg aattccatga tcccctgatt ggcgcggcac agcgtaccgg aatagccgta
     4621 ggcgtccggg tcattctgcg cgtagtgttc gagtttacga atatcgactt tcccaaccag
     4681 cgcggagatg tcctggttgt tctcatcacc gggttccgtt ttggcgatag caatttgttg
     4741 cagaattgac ggccagacct tcactacccg gaacttagtg atatcgccac caaattcatg
     4801 caggcgtttt gccgcccatg gcgacatgat ggtgccgaga taacggcgag gaatgccata
     4861 ctctttttcc agaatctgag catcttcctg cggattgaaa agacagaacg gatgatcgtt
     4921 gaccgggcta cgctcaccgt tggcgctcaa tacataaatc ggtacgagct gcattaatga
     4981 tttcagtcgc tcagcaagcg atgatttacc cccacccaca ggccccagca gatacaggat
     5041 ttgtttcttc tcttccagcc cctgagccgc gtgtttcaga taagagacaa tctgttcaat
     5101 cgcgtcttcc atgccgtaaa actcttcaaa tgccggataa cgtgcaatga cccggttaga
     5161 aaagagtcga gaaagtctgg gttcctgggc tgtatcgacc atgacaggct caccgatagc
     5221 catcaatagc cgctcagccg cgttggcata agcactgcga tcttgccgac aagtggtaag
     5281 aaactcctgc agtgtgaact cttcgtcctt ggcagcttca tatcgctggc gatagtgatc
     5341 gaatatattc atggtatgcc gtcctttcgt tttttagcac aggttaagag ccgttcatat
     5401 gaatagtaga ggctcccgga agaggtaaac tgaacgacgt ctcttgctaa aacacagcaa
     5461 ctctcatgcc attttatcga cgatgagaac aacgtgcccg gtgatacacc ttcaaaatta
     5521 agcgtagatg gcaaatggaa aacttgcctg cacggctttc ttaatttcaa tggcatatca
     5581 acaactcatc cataaatttt gcataattaa tgtaaagacc aggctcgcca gtaacgctaa
     5641 atccatttgg ctgtaagcgc ggtgtcatcc gcggcaggaa aattaaacag ttactttaaa
     5701 aaatgaaaac gtaaaaaggt tgggtttcga tgtattgacg ggtaaacttt gtcgcccgct
     5761 aaacatttgt ttaaggaatg attaattgtg accaaactca aacttctggc acttggagtg
     5821 cttatcgcaa cgtctgcagg cgtagcgcac gctgaaggta aattttccct gggcgcaggc
     5881 gtaggtgtcg ttgagcaccc atataaagat tacgataccg atgtttaccc agtaccggta
     5941 atcaactatg aaggcgataa cttctggttc cgtggcttag gtggtggtta ctacctgtgg
     6001 aatgacgcaa cggataaact ttcaattacc gcttactggt cgccgcttta cttcaaagcg
     6061 aaagacagtg gcgatcacca aatgcgtcac ctggatgacc gtaagagcac catgatggct
     6121 ggtctgtctt atgctcactt tacccagtac ggttacctgc gtaccaccct ggctggcgat
     6181 accctggata acagcaacgg cattgtctgg gatatggctt ggttgtatcg ttacaccaac
     6241 ggtggcctga ccgtgactcc gggtattggt gtgcagtgga acagcgaaaa ccagaacgaa
     6301 tactattatg gcgtatcgcg caaagagtcc gctcgcagcg gtctgcgtgg gtataaccca
     6361 aatgacagct ggagccctta ccttgagctg agcgccagct acaacttcct cggcgactgg
     6421 agtgtttacg gtaccgcgcg ctacacccgt ctgtctgatg aagttactga cagcccgatg
     6481 gtggataaat cctggactgg cctgatttct accgggatca cctacaaatt ctgataatgc
     6541 atctttccag ggcattttgt gcataaaaat agggcgtcac gccctgtttt gcattacggt
     6601 ggtgcaatac ctgggaggaa acaatgcaac aaaaaatgat tcaatttagt ggcgatgtct
     6661 cactgccagc catagggcag ggaacatggt atatgggcga agatgccagt cagcgcaaaa
     6721 cagaagttgc tgcactacgc gcaggcattg aactcggttt aaccctcatt gataccgccg
     6781 aaatgtatgc cgatggcggt gccgaaaagg tggttgggga agcattaacc ggtctgcgag
     6841 agaaggtctt tctcgtctct aaagtctatc cgtggaatgc tggcgggcaa aaagcgataa
     6901 atgcatgcga agccagttta cgccgtctca atactgatta tctcgatctc tacttattac
     6961 actggtctgg cagtttcgct tttgaagaga ctgttgctgc gatggaaaaa ttgatcgccc
     7021 agggaaaaat ccgtcgctgg ggcgtttcta accttggtta tgccgatatg caggaactct
     7081 ggcagctgcc ggggggaaat cagtgtgcca ctaatcaggt gctttaccat ctcggttcac
     7141 gagggattga gtacgatcta ctcccctggt gccagcaaca gcagatgccg gtgatggctt
     7201 acagtccatt agtccaggcc gggcggttgc gcaatggact gttaaaaaac gcggtagtca
     7261 acgaaattgc acatgtccaa caaaatgcag ctgcgcttga ggtcgaactt tcgtcagcgg
     7321 aattagctat gctggataag gcatatccgg caccaaaagg aaaaactgcg ctggatatgg
     7381 tgtgatgttg atacgtgccg gatgcatcgc acccggcaca caacattaac gcaaattaac
     7441 gtttggcaac gcgaatggat tgcgccagat gtgcaggttt ctctttggtg actttttgcg
     7501 tttctgaagc gtaagccgtt tctacacaaa caaaggtctt gtagccatcg tccggcatgt
     7561 cgcccatgct aactgaaagc gccggtcccg ggttccagcc gacaacgttc agatgatgct
     7621 ggtggcctac ggcgataata cgattcagcg cttcatcatt aatcacgctg caatcttgcg
     7681 gattcagata cacgcgatcg gtacggtcag ggaaggtctg aataccgtcg gtcagtacat
     7741 cttctttcgc gtcattcact ttatcaatga agcgatcgcc cagcccactg acgcttacct
     7801 tagcgatatc acccacgtta aagtaggtat gcagggcaga ggtggtttca aattcgccat
     7861 gtgattcaag atcgatttca caggttttac ccacgcggaa atgcgctaac agcgtaaagt
     7921 cgtgcggcca gaattttttc gtctcttcgc tttgtgtcaa ttcaaaagtc agcgctacgc
     7981 catcagcatc ttcatgatgt gatttcagcg tccacggcag gttgcgggca aaaccgtgcg
     8041 caggcagacc ttgttgtgcc gccggaccaa accacggcca gcaaaccggt acgctaccgc
     8101 gaatagcgac gccatttttg aacggtgtgt tgttgctcaa ccacagaact tcttcttcac
     8161 ccgcaggttt ccacgagaga aggtgtgcgc cctgtaatgc aaaagaggct tttacctggg
     8221 gatgatcgac cacaatgagg tccagttcat ccagtttacg acgggagagg acaggggaga
     8281 tttgttcgat gaccggaagg gcaaaaattt tcttaatcat gacgcagtcc tttaacttca
     8341 ttttatcagg taaaaaaaag agcgaccgaa gtcgctcttt ttagatcaca gtgtcatctc
     8401 aacttatttg gagatgtgag cgatcaggtc cagaactttg ttggagtaac cggtttcgtt
     8461 gtcgtaccaa gataccagtt tcacgaagtt gtcgttcaga gcgataccag ctttagcatc
     8521 gaacacggaa gtgcaaactt cgccgttgaa atcggtagat actacgtcat cttcggtgta
     8581 gcccagaacg cctttcattt cgccttcagc agcagcttta acggcagctt tgatctgctc
     8641 gtaagttgca gctttttcca gacgaacggt caggtcaact acggatacgt tcggggtcgg
     8701 aacgcggaac gccataccag tcagtttgcc attcagttct ggcagtactt tacctacagc
     8761 tttagcagca ccggtagagg acgggatgat gttctgggaa gcgccgcggc cgccgcgcca
     8821 gtctttgtga gacgggccat caacggtttt ctgagtagcg gtagtagcgt gaacggtggt
     8881 catcagacct tcgatgatgc cgaagttatc gttgataact ttagccagcg gagccaggca
     8941 gttggtggtg caggaagcgt tggaaacgat gtcctggcca gcatatttgt cgaagttagc
     9001 gcctttaacg aacatcggag tgttgtcttt agacggacca gtcataacca ctttcttcgc
     9061 accagcggtg atgtgtttac gagcagtttc gtcagtcagg aacagaccag ttgcttcagc
     9121 gacaacgtca acacccactt cgtcccattt caggttagcc ggatcacgtt cagcggtaac
     9181 acggattttt ttaccgttaa cgatcagatg accgtctttc acttcaacgg taccgtcgaa
     9241 acggccgtga gtggagtcat atttcagcat gtatgccatg taatcagcgt ctaacaggtc
     9301 gttgattgca acgatctcga tgtcagaacg tttctgagca gcacggaaaa caatgcgacc
     9361 gatacggcca aaaccgttga tacctacttt gatagtcata tattccacca gctatttgtt
     9421 agtgaataaa aggttgcctg taaaattaca aaaaccttac gcagcgtcaa gcggaatcgt
     9481 gtcaatcatt gcgacagatc aatcctgtgc ctaagcatta cgcgactgac tcgcctcact
     9541 cttcctttgg gcttgagacc acatggggtc ggcgccccga attttaaagg gcaattacga
     9601 taaaaatgtg attttcatca cgatttaggt gcagccattt cgtcgagatc aagtttagga
     9661 caaaattttg ccagttggtg ttaatgtttt gttagaatca gtcacgtaat gtgagcacga
     9721 ttaaagtgag atgtgagtaa atggcgaata aaccttcggc agaagaactg aaaaaaaatt
     9781 tgtccgagat gcagttttac gtgacgcaga atcatgggac agaaccgcca tttacgggtc
     9841 gtttactgca taacaagcgt gacggcgtat atcactgttt gatctgcgat gccccgctgt
     9901 ttcattccga aaccaagtat gattccggct gtggctggcc cagtttctac gaaccggtga
     9961 gtgaagaatc cattcgttat atcaaagact tgtcacatgg aatgcagcgc atagaaattc
    10021 gttgcggtaa ctgtgatgcc catctggggc atgtctttcc cgacgggccg caaccaacag
    10081 gcgaacgtta ttgtgttaac tctgcttctt tacgcttcac tgatggcgaa aacggcgaag
    10141 aaatcaacgg ttgaaaaaac gattcagcaa cttattccac aggagcggaa aaattatgaa
    10201 tcttgatgac attatcaaca gcatgacgcc tgaggtatac cagcgtttgt cgaccgctgt
    10261 tgaactgggg aaatggcctg atggcgttgc gttgaccgag gaacaaaaag agaattgcct
    10321 gcaactggtg atgctgtggc aagcccgcca taataccgaa gcacagcata tgacaattga
    10381 cacaaatggt cagatggtca tgaagagcaa acagcagtta aaagaagatt ttggtatctc
    10441 agcaaagcct attgccatgt ttaagtaagt ggttcatccc ctttttgccg cggctatgaa
    10501 ggggatagcc aacaatgacc ccacgactcg tctcaattat ctcgacccga cctggctaag
    10561 tcccagtccg atctcttgca gtcgcgttca cgcctgaatg acttctcctg aacttaagtc
    10621 tcagacctat ttggccggta atccctctcg aatttgctct ttttgcgtcc cgccttccac
    10681 caacctgtaa cagacccagc cattttctgc gtaattgtga tctaagttgt aaaatgcagg
    10741 tcattatctg tgttgtgcag atttaatgtt gacctcaaaa atcatgtggg taagtatgat
    10801 ggccatctaa gcaaaacaaa acacagagga tgacgcaatg aaagcactgg ctcggtttgg
    10861 caaggccttt ggcggctaca agatgattca tgtcccacaa cccatttgtg gcccggaaga
    10921 cgtagtgatt gaaattaaag ccgcggcaat ctgcggcgca gacatgaagc actacaatgt
    10981 cgatagcggt tctgatgagt ttaactctat ccgcggccat gagttcgcag gttgtattgc
    11041 gcaggttggt gaaaaagtca aagactggaa agtggggcaa cgcgtcgtgt cggataacag
    11101 cggtcacgtt tgcggcgttt gtccggcctg tgaacagggt gattttctgt gttgtacaga
    11161 aaaagtaaac cttggtctgg ataacaatac ctggggcggt ggtttttcca aatattgtct
    11221 ggttcctggt gaaattctca aaattcatcg tcatgcgttg tgggaaatcc ctgatggtgt
    11281 tgattatgag gacgcagccg tacttgaccc tatctgtaat gcctataaat ccatcgcgca
    11341 gcaatcgaaa ttccttcctg gtcaggatgt tgtcgtcatt ggcactggcc cactcgggct
    11401 gttttccgta caaatggcgc ggattatggg ggcggtaaat atcgtcgtcg ttggtctgca
    11461 agaagatgtg gcggtccgct tcccggttgc aaaagaactg ggggcgacgg cagtagtaaa
    11521 tggttctacc gaagatgtgg tggcgcgctg ccagcaaatt tgtggcaaag ataatctggg
    11581 actggtgatt gaatgctccg gtgccaatat cgcactgaaa caagccatcg aaatgctccg
    11641 cccgaatggg gaagtggtac gcgttggaat gggcttcaaa cctcttgatt tctcgattaa
    11701 tgacattacc gcctggaata aaagcatcat tgggcatatg gcctatgact ccacctcatg
    11761 gcgtaacgct atccggctat tagccagcgg cgctatcaaa gtcaaaccga tgatcacgca
    11821 tcgtatcggc ctgtcgcaat ggcgcgaagg gtttgatgcg atggtcgata aaaccgcaat
    11881 caaagtgatc atgacttacg actttgatga ataactactc tcctgaagga acgttatcgc
    11941 atggaacaga taacaaaacc gcattgtggt gcacggctgg atcgcttacc ggattgccgc
    12001 tggcattcgt caatgtttgc catcgtcgcg tttggcttgc ttgtctgctg gagtaatgcc
    12061 gttggtggct tgatcctcgc gcagctgaaa gcgttgggct ggacagataa ttccaccaca
    12121 gccacattct cagcaatcac gaccgccgga atgtttctcg gtgcgctggt gggcggcatc
    12181 attggcgaca aaaccggtcg cagaaatgcg ttcatcctct atgaggccat tcatattgcc
    12241 tcgatggttg tcggtgcttt ctcaccgaat atggattttc ttattgcctg ccgttttgtg
    12301 atggacgttg ggctgggagc tttactggtt acactgtttg ctggtttcac cgaatatatg
    12361 cccggtagaa atcgcggaac gtggtccagt cgggtttcct ttattggcaa ctggtcatat
    12421 tcgctctgct cactgattgc gatgggactc acgccgctga ttagtgcaga gtggaactgg
    12481 cgggtacaac tgcttattcc tgcaatattg tcgcttatcg ctacggcgct ggcctggcgc
    12541 tactttcctg aatccccgcg ctggctggaa tcgcgcggac ggtatcagga agccgagaaa
    12601 gtcatgcgga gtatagaaga aggcgtcata cgccagacgg gtaaaccttt gccgcctgtg
    12661 gttattgctg atgacggtaa agcgccacaa gcggtgccgt attcagcctt actgacagga
    12721 gtattactga aacgcgtgat attaggctct tgtgtgctga ttaccatgaa cgttgtgcag
    12781 tacacactaa ttaactggtt gccaacaata ttcatgaccc aggggattaa tttaaaagac
    12841 tcgattgttt taaataccat gagtatgttt ggcgcgccat ttggtatttt tattgccatg
    12901 ctggtgatgg ataaaattcc gcgtaaaaca atgggtgtgg ggctattaat cctgattgcg
    12961 gtgctcggat atatctattc actgcaaacc agtatgttgc tcataacgct gattggtttc
    13021 ttcctgatta ctttcgtcta tatgtacgtt tgctatgcct cggcagtgta tgtccctgaa
    13081 atctggccga cagaggccaa actccgtggc tccggtctgg cgaatgcggt agggcgaatc
    13141 agtggtattg ccgcacctta tgccgttgca gtgctgctca gtagttatgg tgtaacggga
    13201 gtctttattc ttctgggggc tgtttcaatt attgtcgcaa ttgctatcgc caccattgga
    13261 attgaaacca aaggtgtctc cgttgaaagt ttaagtattg atgcagtagc caataaataa
    13321 tacgggtgga ttcaaaatga aaaattcaaa agcaatattg caggtgccgg gcacaatgaa
    13381 aattatttca gcagaaatac cagtgcctaa agaagatgaa gttttgatta aagtagaata
    13441 tgtcggtatt tgtggttcag atgtacatgg ttttgaatca ggcccgttta ttccgcctaa
    13501 agacccaaat caagaaattg gcctgggtca tgaatgcgcc gggacggttg tggctgtggg
    13561 aagccgcgtg cgcaaattta aaccggggga tcgggtaaat atcgaacctg gcgttccttg
    13621 cggtcactgt cgttactgtc tggaaggcaa atataacatc tgcccggacg ttgattttat
    13681 ggcgacacaa cccaactacc gcggcgcatt aacgcactat ctgtgtcatc cggagagctt
    13741 tacttacaaa ctgcccgaca atatggacac gatggaaggg gcgctggtgg agcctgccgc
    13801 agtcgggatg catgccgcga tgctggcaga tgttaaaccg ggtaagaaga taattattct
    13861 gggagcaggt tgtattggtt tgatgacgtt gcaagcgtgc aaatgcctgg gcgcaacgga
    13921 aattgccgtc gttgatgtac tggaaaaacg tctggcaatg ggggaacagc ttggtgcgac
    13981 agtggttatt aatggcgcaa aagaggacac tattgcacgc tgtcagcaat ttaccgaaga
    14041 catgggcgca gatattgttt tcgaaacagc gggttctgcg gtcaccgtta aacaggcacc
    14101 tttaatctgg taatgcgcgg cggtaaaatt atgattgttg gtactgtacc cggcgattcg
    14161 gcaatcaatt tcctcaaaat caatcgcgaa gtcactatcc agacggtatt ccgctatgcc
    14221 aatcgttatc cggtcacgat tgaagctatt tcttcagggc gattcgatgt gaaatcgatg
    14281 gtgacgcata tttacgatta tcgggatgta caacaggcat ttgaagagtc agttaacaac
    14341 aagcgcgaca ttattaaagg cgttattaaa attagcgatt aattaatacg ggagaacaga
    14401 acatgctcgc agatatcaga tattgggaaa acgatgccac aaataaacat tatgcaattg
    14461 cccattttaa cgtatggaat gcagaaatgt tgatgggcgt taaagacgca gccgaagaag
    14521 cgaaatcccc ggttattatt tcttttggta caggttttgt cggtaacacc tcatttgaag
    14581 atttctctca catgatggta tcaatggcac aaaaagcaac ggtgccggta ataactcatt
    14641 gggatcatgg tcggagtatg gagattattc ataacgcctg gactcatggt atgaattcat
    14701 taatgcgtga tgcttctgca tttgatttcg aagaaaatat tcgtttaacc aaagaggctg
    14761 ttgatttctt tcatccgctg ggtattccgg tagaggcgga attagggcat gtcggtaatg
    14821 aaaccgttta tgaggaagcg ctggcgggct atcactatac cgatcctgac caggcggctg
    14881 aatttgttga acgtacgggc tgtgactcat tggctgtcgc catcggcaac cagcatgggg
    14941 tttatacgtc agagccgcaa ttgaactttg aggtcgtcaa acgcgtacgc gatgctgttt
    15001 ctgttccgct ggttttgcat ggcgcatcgg ggatcagtga tgccgacatt aaaactgcaa
    15061 tttcgttggg tattgcgaaa atcaacattc atacggagct ctgtcaggcc gcaatggtcg
    15121 cagtgaaaga aaaccaggat cagcctttcc tgcatttaga acgtgaagta cgtaaagctg
    15181 taaaagaacg ggcattggaa aaaattgaac tgttcggttc tgatggcaaa gcggaataat
    15241 gatatggata atctcgacgt tatttgtata ggtgccgcta ttgttgatat tccattacaa
    15301 ccggtcagta aaaatatctt tgatgtagat tcttatcctc ttgaaagaat cgcaatgagc
    15361 accggggggc gatgcaataa atgaagcaac aattatttct cgtctggggc atcgtaccgc
    15421 attaatgagc cgcattggta aagatgcctc agggcaattt attctcggcc attgtcgcaa
    15481 agagaatatt gatattcaaa gcctgaaaca ggacgtcagc atagatacat ctattaacgt
    15541 tggactggtg acggaggatg gcgagcggac gtttgtcacg aaccgtaacg gcagtttgtg
    15601 gaaattaaat attgacgatg ttgattttgc gcggtttcct caggcgaaat tattatcact
    15661 ggccagtatt ttcaacagtc ctctattgga tggtaaagcg ctaacagaaa tttttacgca
    15721 agcgaaagcc cggcagatga tcatttgtgc cgatatgatc aaaccgcggt tgaatgaaac
    15781 gctggatgat atttgcgaag cattgagtta tgtcgattat ctgtttccaa attttgccga
    15841 ggcaaaatta ctcacaggga aagagacact ggatgaaatt gctgactgct ttcttgcgtg
    15901 cggcgtaaaa acggtggtga ttaaaacggg taaagacggc tgctttatca agcgtggtga
    15961 catgacgatg aaggtgccgg cggtcgcagg aataaccgcc atcgacacca ttggcgcggg
    16021 cgataacttt gcttcaggtt ttattgcggc actgttagaa ggcaaaaatc tgcgtgaatg
    16081 cgcacgcttt gccaatgcaa cggcggctat ctcggttcta agcgtcggtg ccaccaccgg
    16141 cgtaaaaaac agaaagctgg tcgaacaatt gctggaagaa tacgaaggat aatgaaggca
    16201 aatgaaaaag atacctttag gcacaacgga tataacgctt tcgcgaatgg ggttggggac
    16261 atgggccatt ggcggcggtc ctgcatggaa tggcgatctc gatcggcaaa tatgtattga
    16321 tacgattctt gaagcccatc gttgtggcat taatctgatt gatactgcgc taggatataa
    16381 ctttggcaat agtgaagtta tcgtcggtca ggcgttaaaa aaactgcccc gtgaacaggt
    16441 tgtagtagaa accaaatgcg gcattgtctg ggaacgaaaa ggaagtttat tcaacaaagt
    16501 tggcgatcgg cagttgtata aaaacctttc cccggaatct atccgcgaag aggtagaagc
    16561 cagcttgcaa cgtctgggta ttgattacat cgatatctat atgacgcact ggcagtcggt
    16621 gccgccattt tttacgccga tagctgaaac tgtcgcagtg cttaatgagt taaaggctga
    16681 agggaaaatt cgcgctatag gcgctgctaa cgtcgatgct gaccatatcc gcgagtatct
    16741 gcaatatggt gaactggata ttattcaggc gaaatacagt attctcgacc gggcaatgga
    16801 aaacgaactg ctgccgctat gtcgtgataa tggcattgtg gttcaggttt attccccgct
    16861 agagcaggga ttgttgaccg gcaccatcac tcgtgattac gttccgggcg gcgctcgggc
    16921 aaataaagtc tggttccagc gtgaaaacat gctgaaagtg attgatatgc ttgaacagtg
    16981 gcagccactt tgtgctcgtt atcagtgcac aattcccact ctggcactgg cgtggatatt
    17041 aaaacagagt gatttaatct ccattcttag tggggctact gcaccggaac aggtacgcga
    17101 aaatgtcgcg gcactgaata tcaacttatc ggatgcagac gcaacattga tgagggaaat
    17161 ggcagaggcc ctggagcgtt aaatactcaa cacgattcac ctctgtttct gtgaaaactg
    17221 tgatgaaaat ctgtgattca aataggttat tttgaaagta aacatcggtt atgcgataat
    17281 cgcgctaatg tgatgtaaaa agatacaggg gtgtaatcgt ggcggcaaaa gacaggattc
    17341 aggcaattaa gcaaatggtt gccaacgata aaaaggtgac agtctcaaat ttgagtggga
    17401 tttttcaggt aaccgaagaa accattcgcc gcgatcttga gaagctggaa gatgaaggct
    17461 ttttgaccag aacctatggt ggtgctgttt taaatacagc ggtgttgacg gagaatatcc
    17521 atttttataa gcgcgcttca tcgttttatg aagagaagca gcttattgca cgcaaggcac
    17581 taccctttat cgacaataaa accaccatgg cagccgattc cagtagtacc gtaatggaat
    17641 tgctcaaatt gttacaggac cgtagtgacc tgacgttgct aaccaactcg gcggaagcga
    17701 ttcatgttct ggctcagtca gaaattaaag tcgtttcaac gggtggggaa ctaaacaaaa
    17761 atacactttc cctgcaagga agaattacta aagagatcat cagccgctac catgtcgata
    17821 tcatggtaat gagttgtaaa ggtcttgata ttaacagcgg cgcgctggac tctaatgaag
    17881 cggaagctga aatcaaaaag acaatgatcc gtcaggcgac agaagttgcg ttattggttg
    17941 atcactctaa gtttgatcgt aaagcttttg tccagttagc tgactttagt catattaatt
    18001 atataataac tgataaatca ccgggtgcag agtggatagc attttgcaaa gacaataata
    18061 tccagctggt gtggtaattt aagcacacgt tgtcaatatt atttgttcct gcgcctggaa
    18121 ataaaatgat gataaagatg cggcaagtga atttgccgca attgctacct tttatagcaa
    18181 atccgaggca cccaatggaa caatatgatc aaattggcgc aagactggac cgcttgcctt
    18241 tggcccggtt tcattatcgt atatttggta taataagctt tagtctgtta ttaacggggt
    18301 ttttgagtta ctcaggtaat gtcgtcttag caaagctggt aagcaatgga tggtcaaata
    18361 atttcctcaa tgccgccttt acctcggcat taatgtttgg ttatttcatc ggctcactta
    18421 ctggtgggtt tattggtgac tactttgggc ggcgcagggc gtttcgcata aatcttctca
    18481 tcgtcggtat tgctgcaaca ggggccgctt ttgtccctga tatgtactgg ctcattttct
    18541 tgcgcttcct gatgggaaca ggaatggggg cgctgattat ggttggctat gcctcattta
    18601 cggagtttat ccccgcgacg gtgcgtggaa aatggtccgc gcggctctca tttgttggta
    18661 actggtcgcc catgctgtct gcggcgatag gcgtggtggt tatcgctttt tttagttggc
    18721 gaataatgtt tcttctgggt ggtattggca tactgttagc ctggtttctc tcaggtaaat
    18781 actttatcga gtcgccacga tggctggcag ggaaagggca aatcgccggt gcagaaagcc
    18841 aacttcgtga agtagagcag caaattgaaa gggagaagag tattcgttta ccccagctta
    18901 ctttgaacca gagcaacagc aaggttaagg taatcaaggg taccttcagg ctcctgttta
    18961 aaggggaaat gttacgacgt acattagttg cgataactgt tttaattgca atgaacattt
    19021 cgctttatac cattaccgta tggataccga ccatatttgt taactccggc attgatgtcg
    19081 ataaatcaat attaatgacc gctgttatta tgattggtgc tccggtagga atatttattg
    19141 cggcattaat tattgatcat tttcctcgtc gattatttgg ctccgcctta cttattatta
    19201 ttgccgtgtt aggctatatc tattcaagtc agactacaga gtgggcgatt ttaatctatg
    19261 gtctggtgat gatcttcttt ttatacatgt atgtttgctt cgcgtcggcg gtttatatcc
    19321 cggagctttg gccaacgcat ttacgcctgc gcggttcggg tttcgttaat gccgtcggac
    19381 ggatcgtcgc agttttcacg ccctatggcg ttgcggcatt attaacacat tatgggtcga
    19441 tcacggtgtt tatggtgctt ggtgtcatgt tagtgctctg tgcgctggtt ctctccattt
    19501 ttggcatcga aacgcggaag gtgtcgctgg aagagatttc tgaggtgaat taaatctttc
    19561 gttgatggca tcaattgcca gatgtaccac tgacgtgttt tatttggcct acaatgccag
    19621 tacagaattg taggccagcg taaaattacc cctgcgtctc ttcccagtct gccagcgtat
    19681 atagcgttgc cccagctgct gacatctcca taaacgcgtg tgcactgtcc tggggctgga
    19741 tattcacgcc acgacagcca tcggtaatca cgtttacctt ataacctaac tgtaacgcgt
    19801 ccagcacggt aaacttcacg caatagtcag tagccaggcc cataacgatc aattcatcga
    19861 tttcatgatc gcgcaaccag tcatcaagcg cggttttctg ccgacgtccg ttatcaaaaa
    19921 aggcactgta actgtcaact aaaggatttt cgcctttatg gaacaccgct gcgatctctt
    19981 tttgattcag taacggatgt aattgtgcgc cttcactgtt ctgcacacag tgttcaggcc
    20041 agaaagtttg ccgcaggccg tcgagttggc ctggcgtata aggctctacg ccgtgctggc
    20101 tggcaaagct gccgtgattc gccggatgcc agtcctggct ggcgataacc gcttctccgc
    20161 gcgactggca ccagtcaata aggcggttag caacctccac cgtactgtca ccttccggta
    20221 cggcgagcgc gccaccagca cagaaatcat tttgtaaatc gaccagtaac agggcgcgat
    20281 ggggcattgc agtctcctta atcatccagc gtcagttcgc cgcgcaggtt ttggctcatg
    20341 gccttgcgaa tggtttcagt atccagttcc tggctcagca ggtaatgcag tttggttagc
    20401 gtggcttcta cagtcatatc tgcaccgcca ataacgccgg catgggcgag gacgttaccg
    20461 gtggcataac cactcatatt cactttaccg gacatacatt gtgtcaggtt gaccaccaca
    20521 ataccgcgat cgctggcttc ttgtaattcc tgcaggaagg ctttgttttg tggcgcatta
    20581 cccacgccat aggagcgcag aatcaatgct ttcaccggtt ggcgcagaaa attgcgcacg
    20641 acgtcagcag aaatccctgg ataaatcgtc actacgccaa ttggttgtgg cgtgattgga
    20701 tgaacgatta attccccttc accgtgcggg gcgggtggcg tattcaaacg acgaatatgg
    20761 ataccagctt ccagtaacgg aggaaggttt ggagaggcaa acgcatcaaa accatcggca
    20821 tgggctttgg tagtgcggtt gccgcgatac aatcggttat tgaaaaagag cgttacttcg
    20881 ttgatcggat aattcgccgc aacgtacaac gcattcagca gattaatttg tccgtcagag
    20941 cgtaactcag ccagcgggat ttgtgaccct gtcacaatga ccggtttacc gagattctcg
    21001 agcatgaacg acagcgcaga ggcggtatac gccatcgtgt cggtaccgtg tagaatgaca
    21061 aaaccatcat agtcgtcata gtgcgcttta atatcttcag caatatgctg ccagtcttcc
    21121 ggcgtcatat cagaagaatc catcaacggc gtatattcat gaatggtgaa atctggcatc
    21181 tccaggcgat ggaattccgg catcagcgcc agttggcgtt gtagatgacc tgacaccgga
    21241 atataaccct gctcggaacg ctgcatcccg atggtcccgc ccgtgtaggc gacgtaaatt
    21301 gatttctttt gcatgatatt gatatgaatg atatcgaaag agcaaaagta tatacgtgag
    21361 gcaggaggag cgcacctgat actatgcgga taaggaaata tggggcgaga cagcgggaag
    21421 aggattcgtc gaggtaaaaa ataatgtggc cgacgatctg tcggccactc agtacaagac
    21481 ttaacgcacg ttggcgcagg tcaggcaaaa cgcataacgg ttttgtgggt cgttaaacgc
    21541 ggccagcttg tcgctttcac ttttaacggt agaggctacc gaggcaagcg gtgcaggtaa
    21601 catggcctgg aacgcatctg gcaacattgc ccggacagaa ccagacatgt tgtccatcac
    21661 tttgtcgaag aaggtcggtt catcaacgta gtattccaga tgccactgtt tcacttttgc
    21721 gagctctgct gctttggcaa ccgcatcatc gaaatccccg agactatcga ccagcccgtt
    21781 agcttttgca tcctgaccgg tccagacgtg gccctgggcg attttatcaa tttgctccgg
    21841 cgtcgaatga cgcgcatcag caaccagcgt gataaagcgt ttatagccat tctcaatgct
    21901 taattgcatc atctgctgcg cttccggcgg cagtgccctg gtgatagaaa catccgccag
    21961 cggtgaagtt gagacaccat cagtatgaac accaatcgaa tccagactat tttctacggt
    22021 ggtgatcacg ccgaagatac cgatagaacc ggtcagggtg ctggggttag ccacaatgta
    22081 attagctggc gtggaaatcc agtaaccacc agatgccgcc atgccgccca tcgatacaac
    22141 cacagactta cccgctgccc gggctgctgc cagttcagcg cgaatcactt cagacgcggt
    22201 aacgctgccg cctgggctat taacacgcag gacaatcgct ttcactttcg ggtcaaggcg
    22261 agcgtcgcgg atttgtgccg cagtggtatc accgccaaca ttcccctgag tttcctcgcc
    22321 atccataatt gcgccattag cgaagacgac accgatgctg tcaccggtat ctgccggcgt
    22381 tttcaatgcg taatcgtaat aactgatggc gcgataattt ttatcagtct tactccagcc
    22441 gaactctttg gtcagagctt tttcgatttc ggcactcgat gccagtgcat cgaccagctt
    22501 gttttccagt gcatatttcg cggtatcgcc accggttttg gttaaaccct caagcaaccc
    22561 ttgcgcgcca gggaatacct gctgagcagg gatctgccgg ttagcggcaa cagtattcag
    22621 atagttttgc cacagctcac caatccagcg gctgtcagct tcgcgggctg ccggtgacat
    22681 atcatcacga ataaacggtt caacggcaga tttatacgta cccacgcgga acacatgggt
    22741 ggaaactttc agcttatcca gcaacgattt gtagtacaga ccgttggtag caaaaccgtg
    22801 cagatcaacc acgccttgcg gtgacagcca aattttattg gcgaaactgg cgagataata
    22861 ttgcccctgg ctgtagttct cgccaacggc ataaaccggt ttcccgctgt cacgaaactc
    22921 tttcagggct ttgccgatgt actgcataga cggctggtcg ccgcctgcga agtttttcag
    22981 atccatcacg ataccggtga tattgcggtc gtccttcgcc tggcgaatgg tgttgacgat
    23041 atcaaacagt gagttttcct gcagacgatc ggaactggca ccaagcagct ggcggcttaa
    23101 tttactaaac cgctgagaac tgtctggttt atcgacgatc acaccagaaa tgtccagcag
    23161 cagtgcgcca cgactggccg tttctttcga atcaccacca ctgacctgca tccagatccc
    23221 cacaccaacc agtacgagga aaataaagaa caggttaagt accatttcac ggacgaaatt
    23281 cagcagacgc cacgtccatt taaaaaatcc ggcaataaat cgccaaaggg ttcgcatgta
    23341 ttctcccaac ttaaggtcac acctgtcaca ggcgcaatag atgtggttat cgtaatgacc
    23401 caaccggcac ttgtcagcag gaattgcgga ctgtgctgta acaaaatcca ctctcgtgtt
    23461 aattttgtga ataaatatca cgacaggagt taatcaaatg gatgcactcg aactattgat
    23521 caatcgccgt agcgcctccc gcttggctga acccgcgcca acgggtgaac aactgcaaaa
    23581 catcctgcgt gcgggtatgc gtgcgccgga ccataagtcc atgcaaccgt ggcatttttt
    23641 tgtgattgaa ggggaagggt gcgagcgttt cagcgccgta ctggaacagg gggcgattgc
    23701 tgccggtagt gatgataaag ctatcgacaa agcccgtaat gcgccgttcc gcgcaccgct
    23761 catcatcacg gtggtggcga aatgcaaaga aaatcataaa gtcccgcgat gggaacagga
    23821 aatgtctgcc ggatgcgcgg tcatggcgat gcaaatggca gccgttgccc agggatttgg
    23881 cggcatctgg cgcagtggcg cattaactga aagtccggta gtgcgtgaag cattcggttg
    23941 ccgtgagcag gataaaattg tcggttttct ctacctcggc acgccgcagt tgaaagcatc
    24001 tacgtcgatt aacgtcccgg acccgacgcc gtttgtaact tatttctgat aattgtcgcg
    24061 aaactgtctg gattgtgagc aaaggcgcag gaattcagac actctcactt atcacttcac
    24121 ggaatgaggg ttaccatacc cggattgcat tgacaggaga tgtccatgag cgagaactcg
    24181 attcgtttga cccaatacag ccacggagct ggttgcggct gtaaaatttc cccaaaagtg
    24241 ttggaaacca tcctgcacag tgagcaggcg aagtttgttg atccgaattt gcttgtgggt
    24301 aatgaaaccc gcgacgatgc ggcggtgtac gatctgggca atggcaccag cgttatcagt
    24361 accaccgact tctttatgcc gatcgttgat aatcctttcg attttggccg cattgcggcg
    24421 actaacgcca tcagcgatat cttcgcgatg gggggcaaac cgattatggc gattgcgatc
    24481 ctcggctggc cgattaacaa actttccccg gaaattgccc gcgaagtaac cgaaggtgga
    24541 cgctatgcat gccgtcaggc gggtattgcg ctggctggcg gtcactccat cgatgcgccg
    24601 gagccgattt ttggtctggc ggtaacgggg atcgtaccga ccgagcgggt gaagaaaaac
    24661 agtaccgcac aagccggatg caaactgttc ctgacgaaac cgctggggat cggcgttctt
    24721 accacggctg agaaaaaatc actgttgaaa ccagaacatc agggactggc gacggaagtg
    24781 atgtgccgga tgaacatcgc aggcgcgtcc tttgccaaca tcgaaggcgt aaaagcgatg
    24841 accgacgtta cgggctttgg tctgctgggc cacttgagcg aaatgtgtca gggggctggt
    24901 gtgcaggcac gcgtcgacta tgaagcgatc ccgaaactcc ccggtgttga agagtacatt
    24961 aagttgggcg cagtacctgg cggcactgaa cgtaactttg ccagctacgg tcatctgatg
    25021 ggtgaaatgc cgcgtgaagt gcgcgatctg ctgtgtgatc cgcaaacttc tggcggtttg
    25081 ctgctggcgg tcatgccgga agcagaaaat gaggttaaaa ctacagccgc cgagtttggc
    25141 attgaactga cggcgattgg cgaactggtg ccagcgcgcg gcggtcgtgc catggttgag
    25201 atccgttaat tcaatgcggt tgtttattgc cgaaaaaccg agtctggcgc gcgccattgc
    25261 tgatgtcctg cccaaaccgc accggaaagg cgatggcttt atcgagtgcg gtaatggtca
    25321 ggtggtgact tggtgtatcg gtcacctgct tgagcaggcg cagccagacg cctacgacag
    25381 ccgctatgcg cgctggaatc ttgcggattt gccgattgtc ccggaaaagt ggcaattaca
    25441 gccacgaccc tccgtgacca aacaacttaa cgtcatcaaa cgcttcctgc atgaagccag
    25501 cgaaatcgtt cacgccgggg acccggatcg tgaagggcaa ttgctggtgg atgaagtgct
    25561 ggactatctg caactggcac cggaaaagcg ccagcaggtg cagcgttgct tgataaacga
    25621 cctgaacccg caggcggttg agcgggcgat cgaccgtctt cgttccaaca gtgagtttgt
    25681 gccattgtgc gtttctgcgc tggcgcgagc gcgtgccgac tggctgtacg gcatcaatat
    25741 gacccgtgcg tataccattc tcggtcgcaa tgccggttat cagggcgtac tttccgtggg
    25801 acgcgtgcag acacccgtgc tcgggctggt ggtgcgccgc gatgaagaga ttgaaaactt
    25861 cgtggcgaaa gacttctttg aagtcaaagc acatatcgtg acacctgccg atgagcggtt
    25921 taccgctatc tggcaaccga gcgaagcgtg tgaaccgtac caggatgaag aagggcgctt
    25981 gttacatcgt ccactggcgg agcatgtggt taaccgcatt agtggtcaac cggctattgt
    26041 caccagctat aacgataaac gggaatcaga atccgcgccg ctgccgtttt cgctttcggc
    26101 gttgcagatt gaagcggcaa aacgcttcgg tctgagcgcg cagaacgtgc ttgatatctg
    26161 ccagaagctg tacgaaacgc acaagctaat cacttatccg cgttccgatt gtcgctattt
    26221 gccagaagaa cattttgccg gacgccacgc ggtgatgaat gccatcagcg ttcatgcacc
    26281 ggatctgttg ccgcagccag tggtagatcc agatatacgc aaccgctgtt gggatgacaa
    26341 aaaggtcgat gcgcaccacg ccatcatccc gaccgcacgg agttctgcga tcaacctgac
    26401 ggagaacgaa gcgaaggtct ataacctgat tgcccgtcaa tacctgatgc agttctgccc
    26461 ggatgcggtg ttccgcaagt gtgttatcga actggacatt gccaaaggca aatttgtcgc
    26521 taaagcccgt tttcttgctg aagcaggctg gcgcacgctg ttaggcagca aagagcgcga
    26581 tgaagaaaac gacggcacgc cattgcctgt ggtggcgaaa ggcgatgagt tgctgtgtga
    26641 aaaaggtgaa gtggtagagc ggcaaaccca gccgccgcgc cattttaccg atgcaacact
    26701 gctttcggcg atgaccggga tcgcgcgctt tgtgcaggac aaagatctga aaaaggtcct
    26761 tcgtgcgacc gatggtctgg ggacagaagc aacccgtgcc gggattattg aactgctgtt
    26821 caagcgtggt tttctgacca aaaaagggcg ctatatccac tccaccgacg ccggaaaagc
    26881 gttattccat tcgctgccag aaatggcgac gcgaccggac atgaccgcgc actgggaatc
    26941 ggtgctgacg caaatcagcg aaaagcagtg tcgctatcag gactttatgc agccgctggt
    27001 ggggacgcta tatcagctta ttgatcaagc caaacgtacg ccggtgcggc agtttcgcgg
    27061 cattgtggct ccgggcagtg gtggcagtgc tgataagaaa aaggctgcac cgcgtaaacg
    27121 tagtgcgaaa aaaagtccgc cagcagatga agccggaagc ggggcgatag cgtaagcgag
    27181 cgaatctttc gtgctattcg agtcatattc tgaaatatcc agcggatcaa gaaaattcgt
    27241 tggatatttt tttgcatgga taaaattatc gcctctaaag tatgtaataa cagggaatgt
    27301 gatgaaaaaa gttttattac aaaaccatcc tgggagcgag aagtattctt ttaatggctg
    27361 gggaatattt aatagtaatt ttgaacgggt gattaaagaa aataaggcca tgctgctttg
    27421 taagtggggg ttttatttaa catgtgttgt cgctgtaatg tttgtattcg cagcgataac
    27481 atccaacggt ttgaatgaaa gaggcctgat taccgcggga tgctcttttc tttatctatt
    27541 aattatgatg gggcttattg ttcgggccgg ttttaaagca aaaaaagaac aactacatta
    27601 ttatcaggct aaaggtattg agccgctcag tatcgagaag ttacaggcgc tacaattgat
    27661 cgcaccttat cgattctatc ataagcaatg gtctgaaacg ctagagttct ggccgcgaaa
    27721 gcctgaacct ggcaaagata ccttccaata tcatgtgctt ccttttgact cgatcgatat
    27781 catcagtaaa agacgcgagt ctttagagaa tcaattgggt atcgaagata gcgaaagtta
    27841 ttgtgcctta atggagcatt ttctttctgg cgaccatgga gccaatacct ttaaagcaaa
    27901 catggaggaa gccccagagc aggttatcgc cgtgttgaat aaatttgctg tttttccctc
    27961 agactatatc tctgattgcg ctaataatcg ctccggtaaa tcctcggcga agctaatatg
    28021 ggcggcggaa ttatcatgga tgatctcgat atcaagcaca gcttttcaaa acgggacaat
    28081 tgaagaagaa ctggcctggc attatataat gcttgcttct cgaaaggcgc acgagttgtt
    28141 cgaaagcgaa gaagattatc aaaaaaatag tctaatggga tttctttact ggcatatctg
    28201 ctgctatcgc agaaagttaa cggatgctga acttgaagcg tgttatcgtt acgacaagca
    28261 gttttgggag cactacagta agaaatgccg ttggcccata agaaatgttc cctggggagc
    28321 atcatccgtt aaatactcat aaacgcctga aattttgccg ggggcgctgc gcttgcccgg
    28381 cctacattag agcgtaactg caatgaattg tgcccatctg taggcctgat aagcgtagcg
    28441 ccatcaggca tttacaactt aaatcacacc ctgcgccagc atcgcatcgg caaccttcac
    28501 aaaaccggca atgttcgcgc cctgcacgta gttggtttgc tcaccttcac caccatgctc
    28561 aacacaggca tggtggatat ccagcatgat gtgatgcaaa cgtgcgtcaa ctttctcggc
    28621 tttccagccc aggcgcgcag cgttttgtgc catttccagg cccgacgttg ctacaccgcc
    28681 agcattagcc gctttacccg gtgcaaacag tacgcctgcc tgctggaaca gttcagtcgc
    28741 ttcgatggtg gtcggcatat ttgccccttc agcgacggct ttaacgccat tggcgataag
    28801 ctgatgcgcg gtgtcaacat ccagttcgtt ctgggtggcg caaggcaggg cgatatcaac
    28861 cggtacagac cacggctgtt ggccttcgag atagaccaga ccaaattctt tggcgtaatc
    28921 tgccactcga ccatcgcggc tggctttgat ttcgataaga cgtgccagtt tctctttcgt
    28981 gaatccgctt tcatcaacta cagtgccgct ggagtctgac gcagtgatca cacgagcacc
    29041 aaattccatc gctttttcga tagcgtactg ggcgacgttg ccggagccag aaacggaaac
    29101 gcgcatccct tcaaaaccca taccgtggcg ttttaacatt gcttctgtga aataaaccag
    29161 accgtagccg gtagcttccg ggcgaataag actaccgcca aatgaaaggc ccttaccggt
    29221 gaagacgcag gcggtattgt tggagagctt tttcatcatc cccgccataa agccgacttc
    29281 acgaccacca acgccgatat cacctgccgg aacgtcggta tccgcgccca ggtggcgata
    29341 cagttcagtc atcagcgcct ggcaaaaacg catcacttta ccttcgcttt ttcctttcgg
    29401 atcgaaatcg ctgccgcctt taccaccgcc catcggcagg gtagtcaggg catttttgaa
    29461 ggtttgttca aagccgagga atttgagaat ggaaaggtta actgacggat ggaagcgcat
    29521 accgcctttg tacgggccga tggcagagct gaactgcaca cgccatgcac ggttgacctg
    29581 tatctggttg cgatcatcaa cccataccac gcgaaactgg atcacgcgct ccggttcaac
    29641 cagacgctcc agtaatgaca tctggcgata ttttggattt tgttcaagaa aaggccagag
    29701 tgtggtcatt acttcacgaa cggcttgcgc gaactcggtt tgattcgggt cgcgcttttg
    29761 gacatggttg aggaatgact ccagagaata tgtctgatcc atagatataa aaaccttata
    29821 tattaatacg attgtgctta tgttgtttat gtcatgtgct tttgcagttt tcgactatac
    29881 catcaagaat gtgacggtaa gcaagaaaaa aatgacccag gaaagcaaat taataacgag
    29941 agtaatctca taaccaaatg cgataactca gacgctaaag ttttgccacc cggctaccgt
    30001 tatcagtagc agagacaata acgttaagga gttccctgtg agtcgagcat tgttcgccgt
    30061 agtattagca tttccattaa ttgcactggc taacccgcat tatcgtccgg acgtggaggt
    30121 aaacgtaccg ccagaagtgt tcagttcagg cgggcagtca gcgcagccct gtactcagtg
    30181 ctgcgtttat caggatcaaa attattctga aggggcggta attaaagccg aaggaatact
    30241 gctgcaatgc cagcgtgatg acaaaaccct tagcactaat ccgctgctct ggcggcgcgt
    30301 aaagccataa acgcctctaa taatggaatg tcagcagggg ccagcggata ttgcagcgcc
    30361 tcttcaggtg agcaccagac cagcgcctga tgttcatgtg cctgtaacgt cccgtggaag
    30421 tcgggtacgt gccaggcatg aagatggata atccggcccg aaacttctcg ctgatggctg
    30481 gcaacatact cacccacagt tgcttcgatg tccaattctt cgtttaactc acgcaccagc
    30541 gcctgctgct ggctttcatc cagctcgact ttacctccgg caaactccca taatcccgcc
    30601 tgatcgctct gggcggggcg ttgcgcgagt aaaattttgc catcacgttc aatgatggcg
    30661 gcaacaactt caatcatttt cataaattac acacgggcag ataagcgaaa aatgccataa
    30721 tatccgcact gttgctcaat cagaaaggct ttatatttaa ggagtgcctg tgctagaccg
    30781 ccatcttcat ccccggatta aaccgttgtt gcatcagtgc gtgcgggttc ttgataaatc
    30841 gggcattacg ccagatggtt taacattagt gggatttgcc atcggcgtgc tggctctgcc
    30901 gtttctggcg ctgggctggt atctggcggc gctagtcgtc attttgttga acaggctgct
    30961 tgatggtctg gacggtgcgc tggcgcggcg cagagggctt accgacgcgg gcggctttct
    31021 tgatatttct ctcgattttc tcttttacgc gctggtgccg tttggcttta ttcttgctgc
    31081 accggagcaa aatgcgctgg caggcggctg gttgttgttt gcgtttatcg gcacgggcag
    31141 cagttttctc gcctttgctg cactggcagc aaaacatcag attgataacc ccggttatgc
    31201 acataagtcg ttttactatc tggggggatt aaccgaaggc acagaaacga tcttactgtt
    31261 tgtgctggga tgtttattcc ctgcatggtt tgcgtggttt gcgtggtttg cgtggtttgc
    31321 gtggtttgcg tggtttgcgt ggatcttcgg tgcgttgtgc tggatgacga cctttacgcg
    31381 ggtgtggagt gggtatctga cgctgaagtc gctccagcgt cagtaattgc aatgttattt
    31441 gctactgccc gggccgcgtt caccggttgc aaccggattt tttggatcgc tgctccattc
    31501 gtaccagccg ccgtcataca cggaaacatt cttccaaccc atggcgcgtg cgtacataaa
    31561 ggtttcggac gcgcgccagc cggtgccgca gtagaatgaa acttgctgat ctggtttgat
    31621 attccatgct ttccacatag cggtaatatc atcggcgcta cgcatggtgc catccgggtt
    31681 atggaaatct tccatatgcg tcgagtcgct accagcgtgt ccccaacgtg ctccggctat
    31741 ttcacctttt ggtttaatat agctgtaacc gctggtcgta ccgataaatt ctggccacga
    31801 acgaatgctc accagcgatg catcctggcg atgcagcagt ccacgggcct gttccatatc
    31861 gagcatcaac tgcggttgtg ccgggatctt cacgccgaaa tctggttccg ctttcacttt
    31921 cggtggcgtt cctcgctcaa caggcagtcc cgcgtcggac caggtttgcc agccgccatc
    31981 cagcagacgc acatctttca cgcctgcgta gagcataatt tgcgccacac gcgctccagc
    32041 gtatacgtca cgcccgtaca gaatgaccgt ggtgtcatgg cgaatgccgt gttttgccag
    32101 catcgctttt agttgttcat cagaaacttt gttccacagc ggttcgcttt ccacttcgtt
    32161 ggtatcgatg tagtcagcgc caggaatatg gctgataagg taaagcttag gagcgcccca
    32221 ggccgcttca atgactttcc agccaccgac aggtttcgcc gtaacctctt taccttgttg
    32281 caggtcatgc agccattgcg gataaaccag ctgctcaaag tgcggcagtt tttgtagacg
    32341 ggaaggttcg cttagcgcgt cactcagaat ggagatatgc gtaaaacctg ctttttgcag
    32401 tcgcgttttg acggcgtcga catctttgcc attaccgtac agcgccaccg gggcatcggc
    32461 tttcaggtta tgttgcttga tccacgcgtt gagctgttcg gtgctcattt tgtcgagcca
    32521 gctagcagag aggtttaagg cggcaggttc atgaccagaa gggccattta aggtttgtgg
    32581 ccagccgtta taaaacgcgc tggggcgagt atctatcgct ttgccatttt gttgctgaag
    32641 ttggtcaagt gtaagaggct tcgccagttc agcggcccac gaagaagcgc aagccagccc
    32701 taaagccatt gccagcgcgg tcatttgaga aacacgtttc atcgaaaatc ccgacattat
    32761 agttttgagt gcgccattct gaactgacgc attgagaaaa actttgcgtt atcgcagttt
    32821 gttgtaattt tctgaccact gcgccatatc cagaacagaa ctatcagcag gaacatcctg
    32881 gagatcgtgc gttacctgaa cgacggggat cgccagggcg cgaacttcgc tgaacaccca
    32941 ctggcgaaaa ttatcgcgca gagccacatc aagacggctg aatggctcat ccaggagtaa
    33001 cgcttttggc tgggcgagaa gggcgcgtag tagagcaacg cgcgctcgct gaccgccaga
    33061 caaagtggca ggatcctgat ggaaagctcc gtcaaggcct gaacgctcaa gtgcatcatt
    33121 cacggcatta cgtcgggcat tccccttaag tgtcgccggt agcgccagca gtaaattttg
    33181 cccgacgctg aactggtcaa ataacagtgc atcctgaaaa agaatgccaa tctgacgctg
    33241 tgcggtgggt agcatgtcaa tccgttgctc attgagccat agctcacctg tacaagaaaa
    33301 ctgtccggcc agtgcaccaa tcatccatga aaacagagtg gattttccac agccagatgg
    33361 ccccattaac gtgacgatgt cacctttatc aaccgtaaag ttaacgtttg tcagcaagcg
    33421 gctttctggt aaacgtagcg aaacattttt cacgcagagc attagcggat tccttgtctg
    33481 acataaccta cccattttgc gactaacgcg gtcagggcaa aaataataag cggtaacaac
    33541 agttgccata aagcctgggc ggcgagaata ccgttgctgc cgccgctgct taatgccacc
    33601 gcctcagtgg tgagcgtcgg aaaacgcccc gcgcccagcc acagcgtcgg catatactgc
    33661 gcaatactga ctgaaaatcc caccgcgaag gcaatcagca cagggcgcaa catgagtggg
    33721 catttcacgt aaaagaagat tttggcccgc gaccagccca gtgtttgcgc aatcaaaatt
    33781 aaccgtgaat caatgcgctg ccaggcgggt tgcaggataa acagcatcca cggcattacc
    33841 cacagcagat gcccccagac caccgccgtc cagcttccat ccaggttcag ccatagcgcc
    33901 agcgtgtact agcccgccac cagtggcaga gcgggcaata gaatgggcaa ccatagccac
    33961 aactggcgac gctgtgcccc ccattccagc cacagcagta acaggagcaa agcgatgaat
    34021 gttgctacca gccccattgt cagactgttg atgagtgctt cactattgat cgtcgactga
    34081 tccgcgagga tcgccagcag aaccacacac agcacaccgg ttaagggtaa aaaactcgcc
    34141 agcgtagtgc ctggtaataa aggtgtggca ggcttgcgaa tgccatctac gcggggaata
    34201 gtgcgccgcc agctgcgcca tagcagatag ctcagcaaaa cgtaggcggc gagtaacagc
    34261 atcagcagca gactggcgag tgcgcctttc gtttgttgat ccgcgtcgcc ctgagttaac
    34321 cactgccagc taattactgc cagcgtcggt ggattacctg gcccaagaat aatcgccaca
    34381 tccacgaccg aaagcgacca ggcaacaatc gccagcatcg ccattgccag cgaaggcgcg
    34441 acggagggca acagcagcca gttcaggcat tgccagcggc tgtagcccag cgaatccagc
    34501 acaatgacct gctgcaacag ccgtttttcg ctcaatactg ccactaagat ccacagcaga
    34561 aatgcgcttt ctttcactgc aagggtgagg cccagaccga tccaaatcgg tccattggcg
    34621 gagtgaaata cgggaaatag tcatatagca gccctccgtc agcaaagagc agcagggcgc
    34681 tggtggcaaa agccacatgg ggaatggcga gcagccacgg cagacgggcg cacatacgct
    34741 gccatttcgg cccaggccat agcgccacaa tcaccagcag ggcaatcaac aatgccccga
    34801 ccgccgcgat ggttgtcgac accagcgttg ccagtaatgc ctgcggtaac tgtggatcgg
    34861 caaataacgc ctgccagcgt gtcaacgaca acgcaggcga tatcagcgtg agagctgccg
    34921 ggatcagcgg tgcataaatc accgccacca tcgcccacag caaaaagatt aatgcgtacc
    34981 gtaacggcgt aaccattctt gttccagtgc attcacccat cctgcgtgcg gttcagccag
    35041 taccggcggc agatcctgcg gcattcttga ttgcaatatt tcgcgctgcc cgtcaggcag
    35101 tttttgcgga tcgagaacag aaggatcgcc ccagacaacg ggatctgctt tacgcagttg
    35161 cgcatcgggt gagagcagga aattggcaac taccttcgcc gcagcactgg cattcgcgtt
    35221 ggctggaatg gtgacgaaat gcacgttgcc aatcatcccc tcgcgaaagc caaaactgta
    35281 actgcttgcc ggcaaatcgc cgctggcgat tttttgctgc gcatgcgcgg ggttaaaggt
    35341 cagcgacagg cgcaatgtgc cggctttcag cagagcatcc atccgcgcgg gcgaaggcgg
    35401 gaaatccttt ccttcgcgcc acaaatacgg atgcagtgca tcaagatatt gccacaaggg
    35461 cgcagtgaca cgggcgaaag tcgcatcgtc cggcgcttct tttaatgcgg cgggatctgg
    35521 cgtcagcata atcagcaact gttcaagaaa cgccgtgccg gtaaagtccg gtgggcgcgg
    35581 ataagtaacc gtgccgggat tggctttagc aaactccagt aaggcttgcg gcgtttg
//
LOCUS       DAJVQH010000025        35304 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJVQH010000025 DAJVQH010000000
VERSION     DAJVQH010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.025"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..1165)
                     /gene="setB"
                     /locus_tag="OQM87_001357"
     CDS             complement(<1..1165)
                     /gene="setB"
                     /locus_tag="OQM87_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCS1507552.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EIHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFWVM"
     gene            1533..2663
                     /gene="fruB"
                     /locus_tag="OQM87_001358"
     CDS             1533..2663
                     /gene="fruB"
                     /locus_tag="OQM87_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCS1507553.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNCIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2663..3601
                     /gene="fruK"
                     /locus_tag="OQM87_001359"
     CDS             2663..3601
                     /gene="fruK"
                     /locus_tag="OQM87_001359"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCS1507554.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3618..5309
                     /gene="fruA"
                     /locus_tag="OQM87_001360"
     CDS             3618..5309
                     /gene="fruA"
                     /locus_tag="OQM87_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCS1507555.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5733..6674
                     /locus_tag="OQM87_001361"
     CDS             5733..6674
                     /locus_tag="OQM87_001361"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCS1507556.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYVDSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSASKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6662..7600
                     /gene="psuG"
                     /locus_tag="OQM87_001362"
     CDS             6662..7600
                     /gene="psuG"
                     /locus_tag="OQM87_001362"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCS1507557.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQIAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7694..8944
                     /locus_tag="OQM87_001363"
     CDS             7694..8944
                     /locus_tag="OQM87_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1507558.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9015..9713)
                     /gene="yeiL"
                     /locus_tag="OQM87_001364"
     CDS             complement(9015..9713)
                     /gene="yeiL"
                     /locus_tag="OQM87_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCS1507559.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            9844..10785
                     /gene="rihB"
                     /locus_tag="OQM87_001365"
     CDS             9844..10785
                     /gene="rihB"
                     /locus_tag="OQM87_001365"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCS1507560.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMAGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10885..12135
                     /locus_tag="OQM87_001366"
     CDS             10885..12135
                     /locus_tag="OQM87_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1507561.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLVLNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12191..13279)
                     /locus_tag="OQM87_001367"
     CDS             complement(12191..13279)
                     /locus_tag="OQM87_001367"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1507562.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLVDCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13282..14138)
                     /gene="nfo"
                     /locus_tag="OQM87_001368"
                     /pseudo
     CDS             complement(13282..14138)
                     /gene="nfo"
                     /locus_tag="OQM87_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
     gene            complement(14212..15261)
                     /locus_tag="OQM87_001369"
     CDS             complement(14212..15261)
                     /locus_tag="OQM87_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCS1507563.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15360..16241
                     /locus_tag="OQM87_001370"
     CDS             15360..16241
                     /locus_tag="OQM87_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1507564.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16447..17916
                     /gene="lysP"
                     /locus_tag="OQM87_001371"
     CDS             16447..17916
                     /gene="lysP"
                     /locus_tag="OQM87_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCS1507565.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18208..20199
                     /gene="cirA"
                     /locus_tag="OQM87_001372"
                     /pseudo
     CDS             18208..20199
                     /gene="cirA"
                     /locus_tag="OQM87_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20231..21067)
                     /gene="yeiG"
                     /locus_tag="OQM87_001373"
     CDS             complement(20231..21067)
                     /gene="yeiG"
                     /locus_tag="OQM87_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCS1507566.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            21325..21993
                     /gene="folE"
                     /locus_tag="OQM87_001374"
     CDS             21325..21993
                     /gene="folE"
                     /locus_tag="OQM87_001374"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCS1507567.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            22010..23167
                     /locus_tag="OQM87_001375"
     CDS             22010..23167
                     /locus_tag="OQM87_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCS1507568.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            23309..24348
                     /gene="galS"
                     /locus_tag="OQM87_001376"
                     /pseudo
     CDS             23309..24348
                     /gene="galS"
                     /locus_tag="OQM87_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            24628..25626
                     /gene="mglB"
                     /locus_tag="OQM87_001377"
     CDS             24628..25626
                     /gene="mglB"
                     /locus_tag="OQM87_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCS1507569.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25687..27207
                     /gene="mglA"
                     /locus_tag="OQM87_001378"
     CDS             25687..27207
                     /gene="mglA"
                     /locus_tag="OQM87_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCS1507570.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27223..28233
                     /gene="mglC"
                     /locus_tag="OQM87_001379"
     CDS             27223..28233
                     /gene="mglC"
                     /locus_tag="OQM87_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCS1507571.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28304..29539)
                     /gene="preA"
                     /locus_tag="OQM87_001380"
     CDS             complement(28304..29539)
                     /gene="preA"
                     /locus_tag="OQM87_001380"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCS1507572.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29533..30773)
                     /gene="preT"
                     /locus_tag="OQM87_001381"
                     /pseudo
     CDS             complement(29533..30773)
                     /gene="preT"
                     /locus_tag="OQM87_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            complement(30967..31206)
                     /locus_tag="OQM87_001382"
     CDS             complement(30967..31206)
                     /locus_tag="OQM87_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCS1507573.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31209..31928)
                     /gene="sanA"
                     /locus_tag="OQM87_001383"
     CDS             complement(31209..31928)
                     /gene="sanA"
                     /locus_tag="OQM87_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCS1507574.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32078..32962)
                     /gene="cdd"
                     /locus_tag="OQM87_001384"
     CDS             complement(32078..32962)
                     /gene="cdd"
                     /locus_tag="OQM87_001384"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCS1507575.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33092..33787)
                     /locus_tag="OQM87_001385"
     CDS             complement(33092..33787)
                     /locus_tag="OQM87_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCS1507576.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33784..34182)
                     /locus_tag="OQM87_001386"
     CDS             complement(33784..34182)
                     /locus_tag="OQM87_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCS1507577.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34421..>35304
                     /gene="dusC"
                     /locus_tag="OQM87_001387"
     CDS             34421..>35304
                     /gene="dusC"
                     /locus_tag="OQM87_001387"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCS1507578.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVR"
BASE COUNT         8670 a         8630 c         9321 g         8683 t
ORIGIN      
        1 tcattaccca aaacagagtg gcgataatca tcaccatcgc aaaccagaac acagcgtgat
       61 aattccagat ctcggcgacg atgcccgcca ctgatcctgc gatgatccag cccacgcgcg
      121 acgtgttggt atagagcgtg gtggctgaac ctgcctgacc gggcatcaaa tcctgaaaat
      181 agagcatccc gatgccgccc agaatgccaa taaaaatagc atttagcagc tgcaagccca
      241 acagaatggc aggtgaatgc gccatcagca ttcctgcgta aaaacagacg ccacccacgg
      301 cagcaacgcg cattaagaaa cgcttaccca gacgtttggc gaaatatccg gcaatcaaca
      361 tcgtcgggat ttccagcccg gcggcggtcc ccatcatcac accggccagt ttctcgggaa
      421 gatgcagttc gttgataata aatagcggca tgttgatgat gtacaggctg ttcgagcccc
      481 acatcaatgt acaaatgaca aacagcagta gcgtatcgcg acggttacgg cgcggcgctt
      541 caaccgtgcc ggtcgccagc ggaagctctt tttgcatcga cggtaaaaac agccacacca
      601 tcacgccgca aacaataaac gctaccgctg cgctcagata cattaccgta aagctgaaac
      661 ccatcgctaa cgcataagcc agcggtgggc caatgaccca tgccagtgaa acctgagcgc
      721 gtaaaaaaga gctgaacatc accgcctcac gtccggtttt gtcggcatgt tcacgggcaa
      781 gggcaaacat ttgcgggtta gcggtcgagc caaagctgct aagaaagacg ccaacgaata
      841 gcaaaacaaa gtagttgcga ttccaggcaa aaagggtgca ggccagcacg cctaacaggc
      901 agcaaaagac aatcagcgat ttgcgatcgc cgcgcttatc agagcgccca gcgagaaact
      961 gacttaccag aatcccaatg acagcgctgc cggtaaagaa gaatcccacc atcgccggac
     1021 gggcatgtat ttcatcggta aggaaaatac tgagtgtcgg ggtttgcaga gcacccgcaa
     1081 taccggtgag aaaggcaacg attaaaaacg ccgtcgaggt caggtcaaac gatttcgcgc
     1141 tggagactgc gggggagtta tgcatgctgg tcgatcactg gaagaggaag acgcggagtt
     1201 tacgctgctt gtcagaaaat aaacagacag cgaaatcaaa agcgccacga ataatcaaaa
     1261 tgccatttca ctgttgctga ttggctgctg acgctgctga aatgaaattg ctgataacgg
     1321 attttcccat cagcaattag gaaaaatgac aaaaaattgt gcagcacatc aaacttttgc
     1381 tcataacttt acggctttcc ttgcgtgctg aaacagaagt attatgcttt cttgaaacgt
     1441 ttcagcgcga tcttgtcttt aaccctaagc caggttggcg ctttttttct catagaggct
     1501 gaatcgtttc aattcagcaa gagaggagaa ctatgttcca gttatccgta caggacatcc
     1561 atccgggcga aaaggccgga gacaaagaag aggcgattcg ccaggtcgct gcggcgctgg
     1621 tgcaggccgg taatgtagca gaaggctacg tcaatggcat gctggcgcgc gaacagcaaa
     1681 cctcaacgtt cctcggcaat tgtattgcta ttccacacgg cactaccgac acccgcgatc
     1741 aggtgctgaa aaccggcgtt caggtatttc agttcccgga aggcgtcacc tggggtgacg
     1801 gtcaggtagc gtacgtggca atcggtattg ctgccagctc ggataaacat ctgggcctgc
     1861 tacgccagct gacccacgta ctgagcgatg attccgttgc tgaacaactg aagtcagcaa
     1921 caacagcaga agaacttcgc gcattactga tgggcgaaaa gcagagtgag cagctgaagc
     1981 tcgacaacga aatgctgacg ctggatatcg tcgccagcga tctgctgact cttcaggcgc
     2041 tgaacgctgc gcgtctgaaa gaggcgaggg cagttgacgc cactttcgtc accaaagcca
     2101 tcaatgaaca accgctgaat ctcggacagg gtatctggct gagcgatagc gccgaaggca
     2161 atctgcgtag tgcgattgcg gtaagccgtg cggcaaatgc ttttgatgtg gacggcgaaa
     2221 cggcagccat gctggtgagt gtggcgatga atgacgatca gcccatcgcg gttcttaagc
     2281 gtctcgctga tttgttgctc gacaataaag ctgaccgctt gctgaaagcg gatgcggcaa
     2341 cgttgctggc gctgctgacc agcgatgatg cgccgaccga cgacgtgtta agcgcggagt
     2401 ttgtggtgcg caatgaacac ggcctgcatg ctcgtccagg taccatgctg gtcaatacca
     2461 ttaaacaatt taacagtgat attaccgtga caaaccttga tggcaccggc aaaccggcaa
     2521 acggacgtag tctgatgaaa gttgtggcac ttggcgttaa gaaaggtcat cgcctacgct
     2581 ttaccgccca gggtgcagat gctgaacagg cgctgaaagc cattggcgac gctatcgctg
     2641 ctggtcttgg ggagggcgca taatgagcag acgtgttgct acgatcaccc ttaatccggc
     2701 ttatgacctt gttggtttct gcccggaaat tgaacgcggc gaagtgaacc tggtgaaaac
     2761 caccggtctg catgcggcgg gtaaaggcat caacgtggcc aaagtattaa aagacctggg
     2821 aattgatgtc accgttggcg gcttcctggg taaagacaat caggatggtt ttcagcaact
     2881 gttcagcgag ctgggcattg ccaaccgttt ccaggttgta caggggcgca cccgaattaa
     2941 cgttaagctg acggaaaaag acggcgaagt gaccgacttc aacttctcgg gttttgaagt
     3001 cacccccgcc gactgggaac gctttgtgac tgattctctg agctggctcg gtcagttcga
     3061 tatggtctgt gtcagcggaa gcttaccgtc aggcgtcagc ccggaagcgt tcaccgactg
     3121 gatgactcgc ctgcgtagtc agtgtccttg cattatcttt gatagtagcc gtgaagcgtt
     3181 agtagcaggt ttgaaagcgg caccgtggct ggtgaaacct aaccgccgcg agttggaaat
     3241 ctgggcaggc cgtaaactgc ctgaaatgaa agatgtgatt gaagctgcac atgcgctacg
     3301 tgaacaaggt attgcgcatg ttgttatttc actgggtgcc gaaggcgcgc tttgggttaa
     3361 tgcctccggc gaatggatcg ctaaaccacc gtcagtcgat gtcgtaagca ccgttggcgc
     3421 aggggattct atggttggtg gcctgattta tggcttgctg atgcgtgaat ccagtgaaca
     3481 cacactgcgt ctggcgacag ctgttgcagc cctggcggta agtcaaagca atgtgggtat
     3541 taccgatcgt ccgcagttgg ccgcaatgat ggcgcgcgtc gacttacaac cttttaactg
     3601 acagcaggag aggcataatg aaaacgctgc tgattattga cgctaatctc ggtcaggcac
     3661 gcgcctatat ggcgaagacc ctgctgggcg cggcggcgcg aaaagcaaaa ctggaaatca
     3721 tcgacaatcc gaacgacgca gaaatggcga ttgttctcgg tgattccatc ccgaatgaca
     3781 gcgcgctgaa cggtaaaaat gtctggctgg gtgatatttc ccgggcagtt gcgcaccctg
     3841 agctgttcct gagtgaagcc aaaggccatg cgaaacctta cactgcgccg gtcactgcga
     3901 cagcaccggt tgccgccagc ggtccgaaac gcgtagttgc ggtgactgct tgcccgactg
     3961 gcgtagcaca cacctttatg gcggctgaag ccattgaaac cgaagcgaaa aaacgtggct
     4021 ggtgggtgaa agttgaaacc cgtggttctg ttggcgcggg taatgcaatc actcccgagg
     4081 aagtagccgc agcggatctg gtgattgtgg cggcagatat cgaagtggat ctggcgaaat
     4141 ttgctggtaa accgatgtat cgtacctcta ccggtctggc gctgaagaaa acctcgcagg
     4201 aactggataa agcggttgct gaagcaacgc cgtatgaacc ggcgggcaaa gctcaaacgg
     4261 cgaccactga aggtaagaaa gagagtgcag gcgcttatcg tcacttgcta acgggcgtct
     4321 cttacatgct gccgatggtc gttgctggtg gtctgtgtat cgcgctttct tttgcttttg
     4381 gtatcgaagc gtttaaagag ccgggtacgt tggcagcggc gctgatgcag attggtggtg
     4441 gttcagcctt tgcgctgatg gtgccggtac tggcaggtta tattgccttc tccattgccg
     4501 atcgtccggg cctcacgccg ggtctgattg gcggtatgct ggcggtcagc accggttctg
     4561 gcttcattgg cggtattatt gcgggcttcc tggctggtta cattgcgaag ttaatcagta
     4621 cgcaactgaa actgccgcag agtatggagg cgctgaaacc gatcctgatc attccgctaa
     4681 tttccagtct ggtggtcggt ctggcgatga tctacctgat cggtaaacca gttgctggca
     4741 ttctcgaagg gctgactcac tggctgcaga ccatggggac tgcgaatgcg gttctgctgg
     4801 gggcgatcct cggtggcatg atgtgtactg acatgggcgg tccggtaaac aaagcagcgt
     4861 acgcattcgg tgtgggtctg ctgagtactc aaacctatgg cccgatggcg gcgattatgg
     4921 cggcaggtat ggtgccaccg ctggcaatgg gcctggcaac aatggtggcg cgtcgcaaat
     4981 tcgacaaagc gcagcaggaa ggtggcaaag ccgctctggt attggggctg tgcttcattt
     5041 cggaaggtgc aattccgttt gctgctcgtg atccgatgcg tgtgctgccg tgctgtatcg
     5101 tgggtggggc gctgactggc gcaatctcaa tggcgattgg tgcgaaactg atggcaccgc
     5161 acggtggtct gtttgttctg ctgatccctg gcgctattac gccggtattg ggttacctgg
     5221 tagcaattat tgccggtacg ctggtggcgg gtttggccta tgccttcctg aaacgtccgg
     5281 aagtggacgc agtagcgaaa gcagcgtaat aaaaggtgtg ttacagggca gaaatttcct
     5341 gccctgtaac atttgatgag cgcaaagaga atgaccagct tgtgaatgta attttatgaa
     5401 tttaaatggt tttatctttc agtagaaact tcaaacgttc atcaaacact aaggttattt
     5461 cctcaagggc agattatttt ctgccctttt ttagtgttcc tgttctgtgt atcccgtcac
     5521 aaattcctcg cttttgtaat tcaggcattt aatcgcattt tgtgagcctg ctcaaatctg
     5581 cccattttgc ctctctggcg gtaatttact ttacgtaaaa catgcatact atgagcacat
     5641 gtttaaatat gaacacatgt ttaaaccgtc tttgcagtat ctatattctt gtggattgac
     5701 agggttggta attttttacc agaggacgtg ctatgcgcga aaaggattat gtcgtaatta
     5761 taggttcggc gaatattgat gtcgccggat attcacatga atcattaaat tatgtggatt
     5821 caaatccagg taaaataaaa tttacgcctg gtggagtagg gcgcaatatt gcacaaaacc
     5881 tggcgttgct gggtaacaaa gcctggctac tgagcgccgt aggcagtgat ttttatggtc
     5941 aatcgctgct aacgcaaacc aatcaatctg gtgtttatgt cgataaatgc ctgattgtgc
     6001 cgggagaaaa tacgtcgagt tatttatcat tactcgataa taccggtgaa atgctggttg
     6061 ctataaatga catgaatatt agcaacgcta ttacagctga atatctcgca cagcaccgtg
     6121 aatttattca gagggcaaag gtcattgtcg cggactgtaa tatcagtgaa gaggcactgg
     6181 catggattct ggataatgcc gccaacgtac ccgtatttgt cgatccggtt tcagcgtcga
     6241 aatgtgtcaa agtgcgcgac cgtctaaatc agatccacac tctgaagcca aaccgccttg
     6301 aagcggaaac cctgagtggg attgcgctgt cagggcgtga cgatgtggca aaagttgctg
     6361 cctggttcca tcaacatggc ctgaaccgac tggtattgag catgggcggc gacggcgttt
     6421 attacagcga tatcagcggt gaaaatggct ggtctgcgcc gatcaaaacc aatgttatta
     6481 atgttaccgg agcgggcgat gccatgatgg cgggacttgc ttcgtgttgg gtagacggaa
     6541 tgccgtttgc cgaatctgtt cgtttcgcac agggatgttc gtcaatggcg ctctcctgtg
     6601 aatacaccaa taaccccgat ttatcgattg ccaacgttat atcgttagtg gagaacgcag
     6661 aatgtctgaa ttaaaaattt cccctgaatt attacaaatt tctccggaag tgcaggacgc
     6721 tttaaaaaac aaaaaaccgg ttgtggcgct ggaatcaacc attatttctc acgggatgcc
     6781 gttcccacaa aatgcccaga tcgcaattga agtagaagaa actattcgta aacagggcgc
     6841 agtacctgcc actatcgcca ttattggcgg cgtgatgaaa gtgggtttaa gcaaagaaaa
     6901 aattgaatta ctgggtcgtg aagggcataa cgtgaccaaa gttagtcgtc gcgatttacc
     6961 ttttgttgtt gccgccggaa aaaatggcgc aaccactgtg gcttcaacga tgattattgc
     7021 ggcgcttgcc ggaattaaag tatttgccac cgggggaatt ggtggtgtgc atcgcggggc
     7081 ggaacatacc ttcgatattt ctgccgattt gcaagaactg gcaaatacta atgtcaccgt
     7141 tgtttgtgcc ggggcgaaat ctattctcga tttaggatta accactgagt atttagaaac
     7201 cttcggtgtg ccgttaattg gctatcagac taaagcgctg cctgcgtttt tctgccgtac
     7261 cagctcgttt gacgtcagca ttcgtctcga cagcgccagt gaaattgccc gtgcaatggc
     7321 ggtgaaatgg caaagcgggc tgaacggtgg cctcgtggta gcgaacccga tcccggaaca
     7381 gtttgcgatg ccggaggaat ctatcaatgc agccatagat caagccgtcg ccgaagccga
     7441 agagcagggc gttattggta aagaaagtac accgttcctg ctggcgcgcg ttgctgagct
     7501 gaccggcggt gacagcctga aatccaatat ccagctggtg ttcaacaacg ccattctggc
     7561 gagtgaaatt gccaaagaat accagcgtct cgcgggttaa tttctgacgg gcagaaagcc
     7621 gtcgcccgta ctcatcaagc ataccaaaac ctggcgctga cgccgggttt actggcatga
     7681 agggaaaatc attatggata taatgagaag tgttgtgggg atggtggtgt tactggcaat
     7741 agcatttctg ttgtcagtga ataaaaagag catcagtttg cgcacggttg gagccgcact
     7801 gctgctgcaa atcgctattg gtggcatcat gctctacttc ccaccgggaa aatgggcagt
     7861 agaacaggcg gcattaggcg ttcataaagt gatgtcttac agtgatgccg gtagcgcctt
     7921 catttttggt tcgctggttg ggccgaaaat ggatgtcctg tttgacggtg cgggttttat
     7981 cttcgccttt cgcgtacttc cggcgattat tttcgttact gcgctcatca gtctgctgta
     8041 ctacattggc gtgatggggc tgctgattcg catccttggc agcattttcc agaaagccct
     8101 caacatcagc aaaatcgaat cttttgttgc ggttaccact attttcctcg ggcaaaatga
     8161 gatcccggcg atcgttaaac cgtttatcga tcgcgtgaat cgcaacgagt tgtttaccgc
     8221 aatttgtagc gggatggcgt ccattgctgg ttcgatgatg attggttatg ccggaatggg
     8281 cgtaccaatt gactacctgt tagcggcatc gctgatggcg atccccggcg gtattttgtt
     8341 tgcacgtatt cttagcccgg caaccgagcc ttcgcaggtc acatttgaaa atctgtcgtt
     8401 cagcgaaacg ccgccaaaaa gctttatcga agcggcggcg agcggtgcga tgaccgggct
     8461 gaaaatcgcc gctggtgtag cgacggtggt aatggcgttt gtcgcaatta ttgcgctgat
     8521 caacggcatt atcggcggaa ttggcggctg gtttggtttc gccaatgcct ctctggaaag
     8581 tatttttggc tatgtgctgg caccgctggc gtggatcatg ggtgtggact ggagtgatgc
     8641 caaccttgct ggtagcctga ttgggcagaa actggcgatt aacgaattca tcgcttacct
     8701 gagtttctcc ccatacctgc aaacgggcgg cacgctggaa gtgaaaaccg tggcgattat
     8761 atcctttgcg ctttgtggtt ttgctaactt tggttctatc ggtgttgtcg ttggcgcatt
     8821 ttcggctatt tcgccaaaac gtgctccaga aatcgcccag cttggtttac gggcgctggc
     8881 agcagcaacg ctttccaacc tgatgagtgc gactattgcc gggttcttta ttggtctggc
     8941 gtaatgctcg atttgtagac cggataaaac gtattaagcg ttgcatccgg caatcgcgct
     9001 attgcaaata atttttactg catcatcccg gagaatttat tctccgggtc catctccagc
     9061 gccagtcctg acaactgctt tctgttttta attagatatc ctttcttgct tttaattaat
     9121 aaaccgtcgt gaatgaattg cgcgagaaca tataaaagat gtcgataaga aacgcctaaa
     9181 tactctgccg cttgcgtatg cttttcgtga taaagatcac cttcttgtga gagtaatata
     9241 aatgctgcca ggcgattcat taacggaaat gattgattct gagttaaaga aacaatatta
     9301 cgataatttt tatgacttaa ggtgacgcaa agttttcgta aaaatagggt gtcgtttaat
     9361 aacagcggac ggtaatgttt catagggagc gcaaggcacc aacactcttc aatagcctgc
     9421 accgcacgcg gttcatggtc tttgtcgatt aactcaatct cgccaataaa acagggagca
     9481 gcaaagaaat cgatcagcga cacgcgacca ttagcaagcg tggcgtaaag cctggcgcgg
     9541 cctcgcgtca gataaaacag ccatgacggc tgttgccctt cctgcacaat gtagtcacgc
     9601 gctaaaaaat gaaacagccg cgtatcggct gaaacatccg ttgaaaagca atccttaaac
     9661 gcagattcac tcatcaattg ctgcttaagg tcattattgt ggatttcttt cacgatgttt
     9721 atccgctatg agatttctca tatttagcat acgtacgctg ttgttaagat tcaatcatca
     9781 gcccggtcga attttctttg ctgtagaaaa aaataacgca actggaaaca gaggaaataa
     9841 acaatggaaa agagaaaaat tattctggat tgtgagccgg gtcatgatga tgctattgca
     9901 atgatgatgg cagcgaaaca tccggcaata gatttattag gcatcactat tgtagcgggt
     9961 aatcagacgc ttgataaaac attaattaat ggcctgaatg tttgccagaa actggagatt
    10021 aatgttccgg tttatgcggg gatgccgcaa cccattatgc gtaaacaaat cgttgccgat
    10081 aatattcacg gtgaaaccgg actggatggc ccggtattcg agccgctgac ccgccaggca
    10141 gaaagtaccc atgcggtgaa atatatcatc gataccctga tggcaagcga tggcgatatc
    10201 actctggtgc cggttggtcc gctttcaaat atcgcggtgg caatgcgtat gcaacccgca
    10261 atcctgccca aaatccgtga aattgtgcta atggccggcg cttacggtac aggcaacttc
    10321 accccatctg ccgagttcaa tatctttgcc gacccggaag ccgcccgcgt ggtgttcacc
    10381 tccggcgttc cattagtgat gatgggcctc gatcttacca accagaccgt ttgcaccccg
    10441 gatgtaattg ctcggatgga aagggtaggc ggccctgccg gagagctgtt cagcgacatc
    10501 atgaacttca ctctcaaaac gcagttcgaa tactacggcc tggctggcgg cccggtgcat
    10561 gacgccacct gcattggtta tctgatcaac cctgatggca ttaaaaccca ggatatgtac
    10621 gtcgaagtgg acgtcaacag cggcccttgc tacgggcgta cggtctgcga cgaactgggc
    10681 gttcttggca agcccgctaa taccaaagtc ggcatcacta ttgataccga ctggttctgg
    10741 ggcttagtcg aagagtgcgt gcgcggctac atcaaaaccc attaagtgtt gataacaagc
    10801 cgggacagta tcgcaagatg caggcccggc tatttctgat gggcagaaag ccgtcgccca
    10861 aaatgacatg aagagataat cactatggat gtcatgagaa gtgttctggg aatggtggta
    10921 ctgctggcaa ttgcgttttt actgtcagta aacaagaaga agatcagcct gcgtaccgtt
    10981 ggcgcggcgt tagtgttaca ggtcgtgatt ggcgggatta tgctttggtt accgccaggg
    11041 cgttgggtcg cagaaaaagt cgcttttggc gtccacaaag tgatggcgta cagcgacgca
    11101 ggtagcgcat ttatcttcgg ttctctggtc ggaccgaaaa tggatacctt atttgatggc
    11161 gcaggattta tctttggttt cagggtatta ccggcaatta tcttcgtcac tgcactggtg
    11221 agtattctct actacatcgg tgtgatgggg atcttaattc gcattctcgg cggtatcttc
    11281 cagaaagctt taaatatcag caagatcgag tcattcgtcg cggtcaccac cattttcctc
    11341 gtgctaaacg aaattccggc gatcgtcaaa ccctttatcg atcgtctgaa tcgcaatgaa
    11401 ttatttacag cgatttgtag tggcatggcc tcgattgctg gttcgacaat gattggttac
    11461 gccgcactgg gcgtacctgt ggaatatttg ctggcggcat cgttaatggc gatccctggc
    11521 gggatcttgt ttgcccgcct gttaagcccg gctacggaat cttcgcaggt ttcttttaat
    11581 aacctctctt tcaccgaaac accgccaaaa agcattattg aagccgatgc gacaggggca
    11641 atgaccgggc tgaaaatcgc cgccggtgta gcgacagttg ttatggcatt tgtcgccatc
    11701 attgcgttaa ttaacggtat tatcggcggc gttggcggct ggtttggttt tgaacatgcc
    11761 tcgctggagt ccattttagg ttacctgctg gccccacttg cgtgggtaat gggtgttgac
    11821 tggagtgatg caaatcttgc cgggagtttg attggacaga aactggcaat aaatgaattt
    11881 gtcgcttatc tcaatttctc accctatctg caaacggctg gcactctgga tgctaaaacc
    11941 gtggcgatta tttccttcgc gttgtgcggt ttcgctaact ttggttctat cggggtggtg
    12001 gtgggggcgt tttctgcggt tgcgccacac cgtgcgccgg aaatcgccca gcttggttta
    12061 cgcgcgctgg cggcggcgac actttctaat ctgatgagtg ccaccattgc cgggttcttt
    12121 attggtttag cgtagtgtgc aatttatgag ccggattaga tgcggtcaag cgtcgaatcc
    12181 ggcaacattg ttacaccatt ggcactaatg acagcgcgtt atcggcagac agggtagggt
    12241 tgtttaagct gccgctggcg cgtgaaattg ccgcgcaggc catcgcaaaa cgggcgctgt
    12301 cacggaaatt gtttccctcc agaaagctat ataccagacc cgccataaaa ccatcgtcag
    12361 caccaaaact gtctaccgtc gtatgcgctg gcacagtcag caaaaattgt tcaccatcct
    12421 tttcgctgca ataaactgac tcatcgggca aataaacaaa cagttgctga acaccttgct
    12481 gatgcaatgc attcactgcg gcattacggt cagcatcgct ggtgatcgcc tgtccccata
    12541 aaatttccag ctccggtaaa gtgggtttca gggtgtgaat atgcgccagc cagtgtttga
    12601 ttttgcccgc tttgaattct gaaacggtat cgacaaacac cgggatctca tcagcgaggg
    12661 taaagaccca ttccagcgcc tcggctgtca ggttgcaatc aaccagtacc acgcccgcat
    12721 gacgaagtaa atcgcgcgac ccgttcagta gttgcggtgt caactgttcc agcagatggg
    12781 tatcgttaat cgccagcacg gtttgatcgt ctcgattggc aattgccaga tacgtcgatg
    12841 tgctttgacc atgcaaacga acgcagccgg agacattcac gccggcacgg cgcgtttctt
    12901 ccaggagcat ttcgccataa aagtcatcgc caatcactga aagcaaatgg acgtcacggc
    12961 ctaacagcgc cagattgtgg gcgatattgc gtccaacgcc gcctgccgaa caatgaattg
    13021 taccgggatg agaagccgct tgcgggtaat ggatatccgc catcccgcga atatccatat
    13081 tgattgtccc caccactacg cagtattcct gctcggtgag aatgtaacct ttgcctttaa
    13141 tccggccttt gcgcattaaa tccataatat gcgccgcaac gcgcgaacga ctgatttgca
    13201 gcatgtccgc aatttcgttc tgctgaatca gcgggttacg ccgtaaaatt gcaaggatct
    13261 ccttttcccg gttattcatc ttcaggctac cgctttttca gtttgttgcg ctttcagcca
    13321 ggcgatctct tctgcccaga tatccgggtt gatggtttca aggatcagcg gaatgccgtc
    13381 gaaacggtcg ttctgcataa tccagcggaa cgcatcatga ccgatattgc cttcaccgag
    13441 gctatgatgg cggtcaacgc ggctgccaaa ggtacttttc gcatcgttaa ggtgcatccc
    13501 gcgcagatac ttaaagccga caatacgggc aaaatcagcg aacgtttttt cgcattcggc
    13561 tggagtacgc aaatcatacc cggcagcgaa agcatggcag gtatcaatgc agacgccgac
    13621 gcgggattta tcttccacgc cgtcgataat cgccgcgaga tgttcgaatt taaaccctaa
    13681 gttactgccc tgaccggcgg tgttttcaat caccgctgtt acaccttgag ttttatccag
    13741 cgcaatgttg atggattcgg caatacgcgc aaggcaatcc tcttctgaaa tctgcatcag
    13801 atggctgcca gggtggaagt tgagcaaaga aagccccagt gttcgcaacg ctgcatttca
    13861 tctataaagg catcgcgcga tttttccaga gcttcagtga ccggatgtcc gaggttgatc
    13921 agataactgt cgtggggaag aatttgcgcc gatgtgtagt gatatttttc acaggcggct
    13981 ttgaattcat cgatggtttg cgtcgtgagc ggtgcggcac gccactgacg ttggtttttg
    14041 gtgaacaagg caaacgcggt tgcgtcgatt tcggcggcgc gaattgcggc atttgccaga
    14101 ccgccagcag cactaacgtg cgctccaatg tatttcatgc gaggactcct gttaaacccg
    14161 ctggaggaaa acggtaatga tagcgggtta acaggagcaa gaagtagtgg tttatgcgat
    14221 gacgctttga atcacatagt taatcgcacc accaccaaca atcagccagg caaacagtac
    14281 cagtgccatc agcagaggtt tcgccccagc ttttttcagc gcgctgacgt gagtggtcag
    14341 acccagcgcc gccatcgcca ttgccagcag gaaggtatcc agcgttacca gcatgttcac
    14401 cacgctctgc ggtaacaggt ggaacgagtt aaagatggca actacgatga acaagatggc
    14461 aaaccacgga atagtgattt tgcttttctc gccgctgttc gccccagaca gctgtttaac
    14521 acgcgccgcc agcaggatga ggaacggagc cagcatcatc acgcgcagca ttttggaaat
    14581 aactgctgcg ttttctgcat ccgggctgat ggcatgacct gccgccacca cctgcgccac
    14641 ttcgtgcaca gtagaaccaa tgtagatacc gaaagtttcc ggactaaacc attgagacat
    14701 cagcggatat atcgccgggt agaggaaaat cgcgacggtc ccgaagataa caacggttgc
    14761 aacagccacg gttactttac tggcttccgc tttcactacc ggctcagtcg ccagtaccgc
    14821 ggcagcacca cagatactgc taccggcacc gatcaaccaa ctggtgtgct tatccagacc
    14881 aaacactttc tgccccagga agcaagccag caggaaggta ctggacagcg tcagcacgtc
    14941 aatgatgatc ccactgatac cgacatcggc aatttgcgag aacgtcagac ggaagccata
    15001 aagaatgata cccagacgta ataaatattg cttggcaaac agcacaccac cgtcacagct
    15061 tttccagatg tgcggataga tggtgttgcc taaaaccatc cccaacaaga ttgcgagggt
    15121 gagggcacta aacccggcac ccgcaaccgc gggaatggaa ccaccccaca gggcgacccc
    15181 ggtgataact gcactcaggg ctaaccccgg aataaaatgc cacagtgtac gatgttgttt
    15241 ctgtaaggtg atattcgtca taaccctctc ctttaaccgg gcataaggtt acgactaact
    15301 ggtttaaaaa taaaattgat tatatattta taattaatct ttataagtgg taagcgacta
    15361 tgcacatcac cctccggcag ttggaagttt ttgcagaagt attgaaaagt ggatcaacca
    15421 cccaggcgtc ggtgatgctg gcgttgtcgc aatcagcagt gagcgcagcc ttgactgacc
    15481 tggaagggca gcttggcgtt caactgtttg atcgcgtggg gaaaagactg gttgttaatg
    15541 aacacgggcg gctgctctat ccgcgagcgt tggcactgct tgaacaggcg gttgaaatcg
    15601 aacaactgtt tcgcgaagac aacggcgcga ttcgtatcta tgccagtagt accatcggta
    15661 actacattct gcctgcagtt atcgcccgtt atcgccatga ttatccgcag ttgccgattg
    15721 aacttagcgt tgggaacagc caggacgtga tgcaagcggt gctggatttc cgcgttgata
    15781 ttggctttat tgaaggaccg tgccacagca ctgaaatcat ttctgaaccg tggctggaag
    15841 acgagctggt ggttttcgcc gcgccgactt cgccgttggc tcgtggtccg gtcaccttag
    15901 aacagctggc cgctgcgccg tggatcctgc gtgaacgcgg ttccggcacg cgggagattg
    15961 tcgattatct gttgctgtca catttaccga agtttgagat ggcgatggaa ttaggtaact
    16021 ccgaggcaat caaacatgcg gtgcgtcatg ggttgggaat tagttgcctg tcgcgacgtg
    16081 tgattgaaga tcaattgcag gcaggcacat taagtgaagt tgcggtccct ctgccgcgcc
    16141 tgatgcgtac gttgtggcgt atacatcatc ggcaaaaaca cctttccaac gcgctacggc
    16201 gctttctgga ctattgcgat cccgcaaatg tgccgcgtta agttgctgta caagaacatg
    16261 ctggtgctgt gtcgatttcg tgacgcagcg ccttcagcat gcattcgcca gaaaagagat
    16321 tggctgcttt acttataatc cctgggcgat catgaaggtg tcttataacc gtgtatttct
    16381 gccggaagga ttgccaatcg tctgctacaa tcgcgccatc atttttaaga tggatagcat
    16441 ttttgtatgg gttccgaaac taaaactaca gaagcgccgg gcttacgccg tgaattaaag
    16501 gcgcgtcacc tgacgatgat tgccattggc ggttccatcg gtacaggtct ttttgttgcc
    16561 tctggcgcaa cgatttctca ggcaggtccg ggcggggcat tgctctcgta tatgctgatt
    16621 ggcctgatgg tttacttcct gatgaccagt ctcggtgaat tggctgcata tatgccggtt
    16681 tccggttcgt ttgccactta cggtcagaac tatgttgaag aaggctttgg cttcgcgctg
    16741 ggctggaact actggtacaa ctgggcggtg actattgccg ttgacctggt tgcagcacag
    16801 ctggtcatga gctggtggtt cccggataca ccgggctgga tctggagtgc gttgttcctc
    16861 ggcgttatct tcctgttgaa ctacatctct gttcgcggct ttggtgaagc ggaatactgg
    16921 ttctcactga tcaaagtcac gacagttatt gtctttatca tcgttggcgt gctgatgatt
    16981 atcggtatct tcaaaggcgc gcagcctgcg ggctggagca actggacaat cggcgaagcg
    17041 ccgtttgctg gtggttttgc ggcgatgatc ggcgtagcta tgattgtcgg cttctctttc
    17101 cagggaaccg agctgatcgg tattgctgca ggcgagtccg aagatccggc gaaaaacatt
    17161 ccacgcgcgg tacgtcaggt attctggcga atcctgttgt tctatgtgtt agcgatcctg
    17221 attatcagcc tgattattcc gtataccgat ccgagcctgc tgcgtaacga tgttaaagac
    17281 atcagcgtca gtccgttcac cctggtgttc cagcacgcgg gtctgctctc tgcggcggcg
    17341 gtgatgaacg cagttattct gacggcggtg ctgtcagcag gtaactccgg tatgtatgcg
    17401 tctactcgta tgctgtacac cctggcgtgt gacggtaaag cgccgcgcat tttcgctaaa
    17461 ttgtcgcgtg gtggcgtgcc gcgtaatgcg ctgtatgcga cgacggtgat tgccggtttg
    17521 tgcttcctga cctccatgtt tggcaaccag acggtatacc tgtggctgct gaacacctcc
    17581 gggatgacgg gttttatcgc ctggctgggg attgccatta gccactatcg cttccgtcgc
    17641 ggttacgtat tgcagggaca cgacattaac gatctgccgt accgttcagg tttcttccca
    17701 ctggggccga tcttcgcatt cattctgtgt ctgattatca ctttgggcca gaactacgaa
    17761 gcgttcctga aagataccat tgactggggc ggcgtagcgg caacgtatat tggtatcccg
    17821 ctgttcctga ttatttggtt cggctacaag ctgattaaag gaactcactt cgtacgctac
    17881 agcgaaatga agttcccgca gaacgataag aaataagttt tctcccttcc ttgctaagcc
    17941 ctctcaaccg agagggcttt ttcaattcca tttccctgac aaatcatgcg gatataaaat
    18001 ttaacatttg gattgataat tgttatcgtt tgcattatcg ttacgacgca atcaaaaaag
    18061 gctgacaaat cagaggctgt tccggctttc tgggatgatc acctgcatac aaataagtcc
    18121 accgcgatgc tgccgtacgc aaggggacgt aaagaagatg tgagcgatac ccattttatt
    18181 ttcgtagtta cctcatggag atatggaatg tttaggttga accctttcgt acgggtcggg
    18241 ctgtgtttgt ccgctatttc ttgtgcatgg cctgtgttag cggtcgatga tgatggcgaa
    18301 acgatggttg tcactgcatc ttccgtggaa caaaatctta aagatgcacc cgccagtatc
    18361 agcgtcatta cccaggaaga cctgcagcga aaaccggtac agaatctgaa ggatgtcctc
    18421 aaagaagtgc ctggcgtaca actgacgaac gaaggggata accgtaaggg cgttagtatt
    18481 cgtggtctgg acagcagcta caccctgatt cttgtcgacg gtaaacgcgt taactcccgc
    18541 aatgccgtct tccgccacaa tgatttcgat ctgaactgga tcccggtcga ttccatcgaa
    18601 cgtattgaag tggttcgtgg cccgatgtcg tcgctatacg gctccgatgc gctcggcggt
    18661 gtagtgaata tcatcaccaa aaaaatcggt cagaaatggt cgggtaccgt taccgtcgat
    18721 accaccattc aggaacatcg cgatcgcggt gatacctata acggtcagtt ctttaccagt
    18781 ggaccattaa ttgatggtgt gctgggaatg aaagcttacg gcagcctggc aaaacgtgaa
    18841 aaggatgacc cgcaaaactc aacgaccacc gataccggag aaacgccgcg tattgaagga
    18901 ttctccagcc gcgacggcaa tgtcgaattt gcctggacac cgaatcaaaa tcacgatttt
    18961 actgccggat acggtttcga ccgtcaggat cgtgattccg actagctgga caaaaaccgc
    19021 ctggaacgcc agaactactg cgtcagccat aatgggcgtt gggattacgg caccagcgaa
    19081 ctgaaatcct acggtgagaa agtcgagaac aaaaaccctg gcaacagcag cccgataact
    19141 tccgaaagca atacggtcga cggcaaatac acgttgccgc tgacggcgat taatcagttt
    19201 ctcacggttg gcggtgaatg gcgtcacgac aaacttagcg atgcggtgaa cctgaccggg
    19261 ggaaccagct ccaaaacgtc tgccagccag tacgcgctgt ttgtggaaga tgaatggcgg
    19321 atcttcgagc cgctggcgct gacgaccggc gtgcgtatgg acgatcacga aacctacggt
    19381 gaacactgga gtccgcgtgc ctacctggtt tataacgcca ccgacaccgt aacggtgaaa
    19441 gggggctggg cgacggcatt taaagcacct tctctgttgc aacttagccc tgactggacg
    19501 agcaattcct gccgtggcgc atgtaagatt gtgggtagcc cggatctgaa accagaaacc
    19561 agcgaaagtt gggagctggg gctttactac atgggtgaag aaggctggct ggaagggggt
    19621 gaatccagcg ttatcgtttt ccgtaacgat gtgaaagatc gtatcagcat cagccgtacg
    19681 tctgacgtca acgctgcacc gggctaccaa aactttgttg gttttgagac gggcgctaac
    19741 ggacggcgca taccggtatt tagctactac agcgttaaca aagctcgtat tcagggcgtg
    19801 gaaaccgaac tgaaaattcc gttcaacgat gaatggaaac tgtcgatcaa ctacacctac
    19861 aacgatggtc gtgatgtcag caacggcgaa aacaaaccgc tatccgatct gccgttccat
    19921 actgctaacg gtacgctgga ctggaaaccg ctggcgctgg aagactggtc attctatgtt
    19981 tctggtcact ataccgggca gaaacgcgcc gacagcgcga cggctaaaac accgggcggt
    20041 tataccatct ggaataccgg cgcggcctgg caggtgacta aagacgtcaa actgcgcgca
    20101 ggcgtgctga accttggcga caaggatctc agtcgtgacg actacagcta taacgaagac
    20161 ggacgtcgtt actttatggc agtggattat cgcttctgat gagaagatgc ccggcgaacc
    20221 gggcggactt tcacttcagt aaatactgcg catggaagcg caggtgatcc tctataaaag
    20281 aggcgatgaa gtagtaactg tgatcatatc ccggctgaat acgcagcgtc atcggccacg
    20341 ctttctggcg cgcggcttct gccaataccg caggttgcaa ctggtcggca agaaactgat
    20401 cattatcgcc ctgatcgata agcgtcggga tcgcatcctg cgcgttactg gcatacatca
    20461 gcgcgcaact atcccattcc agccatgcat ttttgtcctc acctaaatag gtgctaaacg
    20521 ctttgattcc ccacgggacg ctgcacggat tcacaatggg cgcaaaggcc gaaacgctgg
    20581 tgtatttacc cggatttttc agcgccataa tcagcgcacc gtgaccaccc attgagtgac
    20641 cgctaatggc gcagcggtcg ctgacattaa attgcgactg aaccagcgcc ggtaattcat
    20701 cgcgcagata atcatacatc cggtaatgcg tcgcccacgg cggttgcgtg gcattaagat
    20761 aaaagcctgc gccctggcct aaatcgtagc catcgtcgtt ggcaaccttt tcgccgcgcg
    20821 ggctggtgtc tggcatcacc agtacaatcc ccagttccgc cgctacccgc tgggcacccg
    20881 ccttggtggt gaagttctcg tcattgcagg ttaatccgga aagccagtac agcactggtg
    20941 gcggggtgtg atcacgaggg ggtgggagaa agatactgaa cgtcatcggg cagtttaagg
    21001 tactggagtc gtgtcgccag cgttgctgcc agccttcaaa acagcggtgc tcttcgagca
    21061 tttccatgcg tggctccttg ttgtgttgtt tgcaatttct tcatcatacc gtttattcaa
    21121 tgtgctgtga gtaactttca cttccgtatt tgcataacga tgttttaaca tctgctgatg
    21181 aaaggcagcg gcaattacaa taattatcgc tgtgaatact ggattatgtg cgccgcctca
    21241 cgcacaataa tcaagctgta aatcagctta ataactttgc ccccacgcag ggcggaggcg
    21301 tcacacctgc aggagaaatc ataaatgcca tcactcagta aagaagcggc cctggttcat
    21361 gaagcgttag ttgcgagagg actggaaaca ccgctgcgcc cgcccgtgca tgaaatggat
    21421 aacgaaacgc gcaaaagcct tattgctggt catatgaccg aaatcatgca gctgctgaat
    21481 ctcgacctgg ctgatgacag tttgatggaa acgccgcatc gcatcgctaa aatgtatgtc
    21541 gatgaaattt tctccggtct ggattacgcc aatttcccga aaatcaccct cattgaaaac
    21601 aaaatgaagg tcgatgaaat ggtcaccgtg cgcgatatca ctctgaccag tacctgtgaa
    21661 caccattttg ttaccatcga tggcaaagcg acggtggctt atatcccgaa agattcggtg
    21721 atcggtctgt caaaaattaa ccgtatcgtg cagttctttg cccagcgtcc gcaggtgcag
    21781 gaacgtctga cgcagcaaat tcttattgcg ctacaaacgc tgctgggcac caataacgtg
    21841 gctgtctcga tcgacgcggt gcattactgc gtgaaggcgc gtggcatccg cgatgcaacc
    21901 agtgccacga caacgacctc tcttggtgga ttgttcaaat ccagtcagaa tacgcgccac
    21961 gagtttctgc gcgctgtgcg ccatcacaac tgattaaaag gcaggaacca tggagcgcaa
    22021 cgtcacgctc gattttgttc gcggcgtcgc cattctgggg atcctgctat taaacatcag
    22081 cgcctttggg ctaccaaagg cggcttatct caatcccgcc tggtacgatg ctattacgcc
    22141 gcaggatgca tggacctggg catttctcga tctcatcggc caggtgaaat tcctcacgct
    22201 ttttgcgctg ctgtttggtg ctggcctgca aatgttgctg ccccgtggca gacgctggat
    22261 ccagtcgcgg ttaacgctgt tagtcttgtt gggctttatt cacggtttat tgttctggga
    22321 cggcgatatt ctgctggctt acgggctggt gggcttaatc tgctggtggc tggtgcgcga
    22381 tgcgccatcg gtaaaaagcc tgtttaatac cggcgtcatg ctttatctgg tggggcttgg
    22441 cgttttgctg ttattggggc tgatttccga cagccagacc agccgcgcct ggacgccgga
    22501 tgcatcggct attttgtatg aaaaatactg gaagcttcac ggcggcgttg aagcgatcag
    22561 taatcgtgcc gatggtgttg gcaatagttt actggcactg ggcgcacagt atggctggca
    22621 actggcaggg atgatgctca ttggtgcggc gctgatgcgc agcggctggc tgaaagggca
    22681 gttcagctta cgtcactatc gtcgtactgg ttttgtgctg gtggcgattg gggtgaccat
    22741 taaccttcct gccatcgccc tgcaatggca gctggactgg gcgtatcgct ggtgtgcctt
    22801 cttacttcaa atgccgcggg aactgagtgc gccgtttcag gcgattggct atgcgtcgct
    22861 gttttatggc ttctggccgc aattgagccg ctttaagctg gtgcttgcga tcgcctgcgt
    22921 cggacggatg gcgctgacca actatctatt gcaaacgctg atttgtacca cgctttttta
    22981 ccacctcggt ttgtttatgc attttgaccg cctggagctg ctggcgtttg ttattccggt
    23041 atggctggcg aatatcctct tctctgttat ctggctgcgt tacttccgcc aggggccggt
    23101 ggaatggctc tggcgtcagt taactttgcg tgctgccgga ctggcaatat ctaaaacatc
    23161 aagataacga tctggatcac attcgttaac aaaacggctg taaccgtttc cattgctgtg
    23221 actcgattca cgaagtcctg tattcagtgc tgacaaaata gccgccagca agcagtcatt
    23281 tactgcaatc tcataacagg tagtgaatat gatcaccatt cgtgatgtag cgcgtcaggc
    23341 tggcgtctct gtggcaacgg tttcccgggt gctcaataac agcacgctgg tcagtgccga
    23401 cacgcgtgaa gcagtaatga aagccgtgag tgagctggat tatcggccaa acgccaatgc
    23461 ccaggcgctg gcaactcagg ttagcgacac cattggcgtg gtggtgatgg acgtttctga
    23521 tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc gcccagcagc atcagaaata
    23581 cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag cgtcacgcca ttgaggtgtt
    23641 aattcgccag cgttgtaatg cgttgattgt tcactcaaaa gcattgagtg acgatgaact
    23701 ggcacaattt atggataaca ttcccggtat ggtgttaatc aaccgcgttg tgccggggta
    23761 cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc cgaatggcga cgcgcatgtt
    23821 gctgaataac ggtcatcaac gtattggtta tctttcttcc agccacggca ttgaagatga
    23881 cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag caggatatta ttccgccgga
    23941 aagctggatt ggcactggta cgccggacat gccgggcggt gaggcggcga tggttgaact
    24001 gctggggcgc aatctacaac ttaccgctgt atttgcttat aacgacaata tggccgctgg
    24061 cgcactgaca gcattaaaag ataatggcat tgcgattccg ttacatctct caatcatcgg
    24121 tttcgatgat attcccatcg cccgttacac cgacccgcaa ttaacgaccg tgcgttatcc
    24181 cattgcttca atggctaaat tagccaccga actggccttg cagggggcag caggcaatat
    24241 tgatcctcgt gccgccactg ttttatgccg acgttagtgc gtcgccattc tgtcgcaacg
    24301 cgccagaatg cggcggcgat cactaactca acaaatcagg cgatgtaacc gctttcaatc
    24361 tgtgagtgat ttcacagtat cttaacaatg tgatagctat gattgcaccg ttttaatgtt
    24421 gtaacccgta tgtaacagtg aataatcact ttcaccgagg taatagcgtc ataacaacaa
    24481 ttaaagccgt tttctggagc gttaccgggc atggaagaac gaattttaaa aagtgagctt
    24541 cggcgttcag taacacttca ttaactctac tgccccgccg agcatttatc tcaagcacta
    24601 ccctgcataa gaaaaaccgg agataccatg aataagaagg tgttaaccct gtctgctgtg
    24661 atggccagca tgttattcgg tgccgctgca cacgctgctg atactcgcat tggtgtaaca
    24721 atctataagt acgacgataa ctttatgtct gtagtgcgca aggctattga gcaagatgcg
    24781 aaagccgcgc cagatgttca gctgctgatg aatgattctc agaatgacca gtccaagcag
    24841 aacgatcaga tcgacgtatt gctggcgaaa ggggtgaagg cactggcaat caacctggtt
    24901 gacccggcag ctgcgggtac ggtgattgag aaagcgcgtg ggcaaaacgt gccggtggtt
    24961 ttcttcaaca aagaaccgtc tcgtaaggcg ctggatagct acgacaaagc ctactacgtt
    25021 ggcactgact ccaaagagtc cggcattatt cagggcgatt tgattgctaa acactgggcg
    25081 gcgaatcagg gttgggatct gaacaaagac ggtcagattc agttcgtact gctgaaaggt
    25141 gaaccgggcc atccggatgc agaagcacgt accacttacg tgattaaaga attgaacgat
    25201 aaaggcatca aaactgaaca gttacagtta gataccgcaa tgtgggacac cgctcaggcg
    25261 aaagataaga tggacgcctg gctgtctggc ccgaacgcca acaaaatcga agtggttatc
    25321 gccaacaacg atgcgatggc aatgggcgcg gtagaagcac tgaaagcaca caacaagtcc
    25381 agcattccgg tgtttggcgt cgatgcgctg ccagaagcgc tggcgctggt gaaatccggt
    25441 gcactggcgg gcacagtact gaacgatgct aacaaccagg cgaaagcgac ctttgatctg
    25501 gcgaaaaacc tggccgatgg caaaggtgcg gctgatggca ccaactggaa aatcgacaac
    25561 aaagtggtcc gcgtacctta tgttggcgta gataaagaca acttggctga attcagcaag
    25621 aaataagact gattgggcgc agtcgtttac tgcgccctta ttgtatggcc aacaaggtaa
    25681 aattatatgg tcagctcaac gactccgtct tccggggaat acttgttgga aatgagcggt
    25741 atcaacaagt cctttcctgg tgttaaggca cttgataacg ttaatttaaa agtccggcca
    25801 cattctatcc atgcattaat gggggaaaac ggtgcaggaa aatcgacatt attaaaatgc
    25861 ctgtttggta tttatcaaaa agactccggc accattttat tccagggtaa agagatcgat
    25921 ttccattccg ccaaagaggc actggaaaat ggtatttcga tggtacacca ggagttaaac
    25981 ctggtattac aacgttcggt gatggataac atgtggctgg ggcgatatcc caccaaaggc
    26041 atgtttgtcg atcaggacaa aatgtaccgc gaaaccaaag cgatttttga tgaactggat
    26101 attgatatcg atccgcgtgc gcgcgtcggc acattatccg tttcgcaaat gcagatgatc
    26161 gaaatcgcca aagcgttttc ctataacgcg aaaattgtga ttatggatga accgacttct
    26221 tcgttaaccg aaaaagaggt caatcatctg ttcactatta ttcgtaaatt aaaagagcgc
    26281 ggctgcggta ttgtttatat ctcgcataaa atggaagaaa tcttccagtt atgtgatgaa
    26341 gttaccgtat tgcgcgacgg tcagtggatc gccaccgaac cgctggcagg actgacgatg
    26401 gacaagatca tcggcatgat ggttgggcgt tctcttaatc agcgttttcc tgacaaagaa
    26461 aacaagccgg gcgaagtcat cctcgaggtg cgtaacctaa cgtcactgcg ccagccgtcg
    26521 attcgcgatg tctcgtttga tctgcataaa ggggagatcc tcggtattgc cgggctggtg
    26581 ggggcgaaac gtaccgatat tgttgagacg ttatttggta ttcgcgagaa atcggctggc
    26641 accattacgt tgcacggcaa acagatcaat aaccataatg ccaacgaagc cataaaccac
    26701 ggatttgcac tggtaactga ggagcgccgc tcaacgggaa tttatgccta tctggatatt
    26761 ggttttaact cgttaatttc caatattcgc aactacaaaa ataaagttgg tttactggat
    26821 aactcgcgga tgaaaagcga tacccagtgg gtgattgatt cgatgcgggt aaaaacgccg
    26881 ggtcatcgga cgcaaattgg ttcgctctcc ggtggtaatc agcaaaaggt gattattggt
    26941 cgctggctac taacgcaacc agaaatatta atgctcgatg aaccgacgcg cggtattgat
    27001 gtcggggcga agtttgaaat ttatcagtta attgccgaac tggcgaagaa aggcaagggg
    27061 attattatta tctcctctga aatgcctgag ttgttaggga taacagaccg tattctggtc
    27121 atgagcaatg gtctcgtttc cggaattgtc gatacaaaaa caacaacgca aaacgaaatt
    27181 ctgcgtcttg cgtctttgca cctttaagat taggggcttc ccatgagtgc gttaaataag
    27241 aaaagttttc ttacttacct gaaagagggc ggtatttacg tcgttctttt agttttgctg
    27301 gcgattatta ttttccagga cccaacattt ttaagtctgt tgaacttaag taatattctc
    27361 acccagtcat cggtgcgtat tattatcgcg ctcggtgtgg cagggttaat tgtcacccag
    27421 gggaccgatc tttctgctgg tcgtcaggta gggctggcgg cagtggtggc tgcgacatta
    27481 ttgcagtcca tggataacgc caacaaagtg ttcccggaaa tggcgacgat gccgattgcg
    27541 ctggttattc tgattgtctg tgccattggt gcggtgatcg gtttgatcaa cggtctgatt
    27601 atcgcttatc tcaacgtgac gccgttcatt accacgctcg gcacgatgat catcgtctat
    27661 ggcatcaact cgctctatta cgactttgtc ggggcgtcgc caatttctgg ttttgacagt
    27721 ggcttctcta cctttgctca gggctttgtc gcgctgggga gtttccgtct ctcttacatc
    27781 accttctacg cgttgattgc ggtggcgttc gtctgggtgt tgtggaacaa aacccgcttc
    27841 ggtaagaaca tttttgccat tggcggtaac ccggaagcgg caaaagtatc tggtgtcaac
    27901 gtcggcctga acctgctgat gatctacgcg ttgtctggcg tgttctatgc ctttggcggg
    27961 atgttagaag ccggacgtat cggctctgcc accaacaacc tcggctttat gtatgagctg
    28021 gatgctatcg cggcgtgcgt ggtaggcggt gtatcgttca gcggcggtgt ggggacggtg
    28081 attggcgtgg tgaccggggt aattattttt accgtcatca actatggcct gacgtatatc
    28141 ggcgtaaacc catactggca gtacattatc aaaggggcga ttattatctt cgccgtagcg
    28201 ctggattcac tgaaatacgc gcgtaagaag tgatgtgatt gcggatattg ccggatgcgc
    28261 tgcatccggc aatcggtgcc aaatgccgga tgcgtctctg gttttacaac gttaccgggt
    28321 gttctttctc gcctttctta aacttcactt ccccgagctc aatacaaccc accgggcaga
    28381 cgtgaccaca aagcagacaa cccacacatt tctcggtatt acaatgcggt gtgcgggttt
    28441 tctcgctcca ttccatcgcc tggtgaccgc cgtcgtaaca ggaaatataa cagcgtccac
    28501 agccaacaca tttgtcaaga ttgatacggg gatagacaat ataactgcgg tctaaatctt
    28561 ccgccgggac aatattgtta ttcgccagac ctaccatctc ctgcaacgaa tcaaatccct
    28621 gatcggcgag ataatgcgat aacccgctcg ccatatcttc tacgatgcga tagccgtact
    28681 gcataatgcc ggtggtgacc tgtaacgttg ctgcgccgag caataaaaac tccgccgcat
    28741 cttcccaggt ttcaatgccg ccgataccgc tgattgggaa atcgcgcagt tctggatggg
    28801 tgcgcatttg ctgaatgaag cgcagggcga tcggttttac cgctttaccg gaatatccgg
    28861 aaatactcga tttaccgtta acgatcggca taccgatttt ctgattaaga tcgatattgg
    28921 tgatggattt aacggtgtta attgccgcaa tgccatctgc gccgccgcgc ttcgccgcca
    28981 gcgccacttc gcacatatca ccgatattcg gcgtcatctt cgccagcatt ggcagcgtgg
    29041 aaccccgttt cactgcccga caatattttt ttaccagctc cgggctttgc ccgacatcgc
    29101 tacccatcgc atgagaagtc atttgcggac aggagaagtt acactcgatc atatccgcgc
    29161 cagcttcttg caccaggcgc gccagctcct cccattgctg ctcattttcc cccatgatcg
    29221 aagcgatcaa tactttgtcc gggtaatctt ccttcagccg acgcagggcg gtcagattct
    29281 cttccaacgg atgttcagca atctgctcca tatttttgaa gccgataaaa ccggtatctt
    29341 ctttcaccag atgatcaaaa cgcggcgaga cttcgttggc gataaaaaag ccgatcgttt
    29401 taaacaccac accgccccaa cctgtgtcgt aggctttggc acacatctca tagcagttgc
    29461 ctaccggcga agaagagagg cagaacgggt tgggaaagtt cacgccgcaa aaagtaatcg
    29521 aaagatcttt cgttaacatg agcaagctcc ctctaatata gtgatgaatc gcctcggcgg
    29581 cttctttccc ggttttcacg gcatagacca cggttttgtc accctcaaca atatcgccag
    29641 cagcaaagac ttgcggatcg cgggtctgat aattttgtgt tttgatggtg ttacgctgct
    29701 gctctaactc cgcaaaggca tccagtctgg catgctgacc gacggcgaga ataattttat
    29761 ctgccgccat cgtcagttcg cctgataacc gtacatgctt aaacgtgact ttattgcctt
    29821 ccacggctac tggcgtgaat ccatcgatga tcgaaacgcc cagctcccgg gcgctggcaa
    29881 actctttttc gcttgccgga aactcatcta actcttcacg tgctacgcaa gttaccgcct
    29941 gacagccgag aactttcagc gtgctggcta cgtccatcgc gacatcaccg ccgccgataa
    30001 ttaatgcgct ttgtggaatg ctgatatcgc cttgcggctg tcgtacacgt tgcaggaagt
    30061 cgacggcaat ctcaacggtc gctatgctca aacagcggta gcccggaacc gcttgataac
    30121 cccacggtga ccagtaccgc gcggttttcc gctttcagtt gctccagggt gagtgtttta
    30181 ccgatttcgt tattgcactt aatggtcacg cccatttttt caatacgggc gatctctgca
    30241 tccagcactg attgcggtaa ccggaattgc ggaataccgt tacgcaacca gccaccgggg
    30301 tgcgcttctt tctcataaat cgtgacgtca taaccctggt ttgtcagtgt cacactggcc
    30361 tgcaatccgg caggacctgc gccaataatc gcgactttgc cgagcgtttt agtaccgggc
    30421 tgataaattt ccatcccggt ttgttgttca aaatcagtaa caaaacgctg taagcggccg
    30481 atatcaatgg gagcatcaac accggcacgg gtacaaccgc tttggcataa tttctccgtc
    30541 gggcacactc tggcacaaac ggcaccgagg gcattatttt cgcgaattgt ctcggcagcg
    30601 cctttaaaat tacgaaagta gattgagcga ataaatttcc ccggatcggt ctgtgccggg
    30661 caagcctgac tacagggagc gtcgtgacat aataaacagc gagaggcttc tttaatcgcc
    30721 agtaacggcg taaaagccgg agtgagttca tccagataat tttgttgcgg catgtgatct
    30781 tccttaatgc tatgagtaat tatggcgaac gctaaataaa tattgcgtgc tgatattaag
    30841 gctatgcgcg tggggaaatt taaactgtga aaggattcac aaatagctgc ggttcagaat
    30901 gttatatccg cagcttattg agtgaagtaa taattattct caataattaa cagaaaagag
    30961 aaaatattaa cgatagaaaa taagataaac aatcatgccg atagataaaa ttccgaagcc
    31021 aatatatgcc accatataag cgaagaagag tccgggattt tttgctcgcc agacatacac
    31081 cggttccggt gcatttttaa cccgtttatc aatcgcgcct gcgcgccagc ctttccatgc
    31141 ttcgcggaaa caaaatatcg ggataaggaa aaaaacggca acgacaaaaa actgctgtac
    31201 atccataact actttccttg tttcttttgt aattcaagta actgttcggg tgtaacggcg
    31261 ggatacccct gcgcatcttc cggcacctgg tgcatagccg gaatagggac cagcggccct
    31321 aaaaaacgcg gttcacgttt aaaaatataa aggtcagcca gcgcaccgaa acgggcggca
    31381 aattcgcgaa tacgtactga cagcatatct ttcggtgacg gtacggcata acactgagcc
    31441 tgaatcccca tatgcagcgc aataaataat gctcgctcac agtggaaacg ttgggtgata
    31501 ataatgaaat cattagtatc gaaaactttg cgtgtacgca cgatggaatc cagcgtacga
    31561 aagcctgcgt aatcgagaac aatatctgat gggtcgacac cagcagcgat taaatctttg
    31621 cgcatggtca tcggctcgtt ataactttgc aatgcgttat cgccgctcag taatagataa
    31681 tttaccttac cgctgttata ggcattaatc gctccttgaa tgcggtagcg ataatactga
    31741 ttaataacgc cggtacgata atattttgct gtgccgagca ccacgccgac ctggcggtag
    31801 gggagatcct gcaattcgtc gtagatataa ggcgcggttt tccagctcat ccagcgatcg
    31861 aggccgagca cagtcaacag cagcaagccg atcaggacta acaggctgag gaacacgcgc
    31921 tttaacatgc aggagactcg agctaaggaa aatttccatc aggctacttt acccgctggt
    31981 tttgcgcaag aaacggcaaa ctgtttgccg ggaatttcga caccaggaga gggctggcaa
    32041 gccggacaag gcgttcacgc cgcatccggc accaggctta agcgagaagc acgcggtcga
    32101 tactgtgaca gccgagagct ttcagcgttg cggaggtggc atcccactga atcaacggcg
    32161 catcggcttt ttctgccaga accgcgcgct ggatatccgg gtaatcataa cccttgagat
    32221 tcaacagaat taacgccccc tgcaacggcg gcagagtcgg gttgaatgcg gcgttttcag
    32281 cgtagctgcc gctgaaaata cgaccgtctt tacattccag cgcgacaccg cttggtgact
    32341 tactgtaagg catgtgcgaa cggtttgccg ctgcaatcgc cgcctgagaa agcgcatcac
    32401 cagtcagcgc atagccgtga tcctgctcgt ccatcagcag cgttttaatc tccagatctt
    32461 tcggcccaaa ggcatccggc agatagtcac gcagcgcgtg tgcctcgcgg cccggcaaat
    32521 gaatacgcag atccagaccg ctgttcagtt cattcataaa ctgacggcag tgaccacaag
    32581 gcgtatagtt aacggtgatg gctgcaagcg ctttttcacc actcaaccag gcgtggctga
    32641 tcgcgctttg ttcggcatga acggtttgct gcattgttgc accaataaat tccatattgg
    32701 caccaaaata ccaggttccg ctcacaccgc gcgcaattgc gccaacatta aaattcgaca
    32761 atggcgtacg cgcacaggcc gccgccagcg gaagtagtgc gaatgccagc gcgtcttcgt
    32821 ccagccccgt tgcgctcttc agcgatgaga cttgctcccc ggtcaacaaa gcggggaagt
    32881 acttgtctgc cagaataggt tccagtgcag attgcaagtt atccgcaagt tgggcaaaag
    32941 cggtttgaaa acgtggatgc atggcgttgc ctcataatca tgtaatggga taacagttta
    33001 cgaacccgtt atggctttat atgtgatctg aatctcatta attatgcaac acgtgaaact
    33061 tacatcaaaa atgcgcgacg tatcgcaaat tttagcccat tactgccaga ataatcggga
    33121 aaaggaacgg tgcgatcagc gaagtaatta tcccgcataa caccagcgct agcgaactaa
    33181 atgcaccttc ctgataatcc agctcggcgc agcgcgccgt accgagggcg tgcgaggcag
    33241 tccccattgc cagaccgcgc gcagctttgg tacgaatacg catcgcatta agcaatgtat
    33301 ggccaaatac cgcgccgagg atgccgacga aaatcacgca aactgcgctg attgccggaa
    33361 taccgccaat actgccgcca accgccattg caataggcgt ggtgactgat ttcggcagga
    33421 ttgacgcggc gatttccggt gaagcgccca tcaacaatgc cacggaagtc cccgtcacca
    33481 tcgcaaccac gctgccgata aaacagatgg tgatgatcga tttccagcgc gcgcggatct
    33541 ggtgtagctg ctcatataaa ggataggcca gcgcgacgac cgccggttgc agcagatcgt
    33601 ttagcacttc gctaccttta aagtaactgt cgtaagagat gccggtcagc attaaaaaag
    33661 gaatgatcac caccattgct accagcaacg ggttaagcag gggaaactta taccgtgccg
    33721 ccagtttgcg ggcggcaaaa aagacaatca acgtcagcgg taatgaccac cagatattcg
    33781 ccatcattct tctgatcctt tctgacctac gactttacgt tcaccgtgta ccagttgtga
    33841 actccagctc accaccagaa aaaccaccag cgtactgaca gcacaggaaa ccactaccgg
    33901 gccaaactgc gcgcggagca aatcaaaata ttgcatgacg cctacgccaa tcggcacaaa
    33961 caatagcgcc atatagcgaa tcagtacgta gcaccccgga ttgacccatt tcgccggaag
    34021 aatttgcaag gccagcagga caaacaggat cagcatcccg atgatgctgc ccggaatggt
    34081 taccggtagc agggaagcaa tgaaaatgcc tgcatacagg caggcataaa tcaggacgaa
    34141 agcgcgtaaa tattgccaga taatgttcag tgtcttactc ataatcaaca ccccagatga
    34201 gatggattca tcatacaatt aattcgtaaa acgtgctacc gatcacatta agaaatcagc
    34261 gcttattttt tcgctacccg gtattacgca atgagcagcg aactcaggca tacatcaagc
    34321 atagagaggg attgcagttt gcagcaatcc ctctttgcag acctttaatc cgctgaccat
    34381 aggcgctaac atagcgcctc atttttttgc gggtgatgat atacgtgtgt tactggcacc
    34441 gatggaggga gtgcttgact ctctggtgcg tgaattgctg accgaagtta acgactacga
    34501 tctgtgcatc accgagtttg tccgcgtggt ggatcaactg ctgccggtaa aagtctttca
    34561 tcgcatttgc cctgagctac aaaacgccag ccggacacca tctggtacgc tggtgcgcgt
    34621 gcagttgtta ggtcagttcc cacaatggct ggcagagaac gccgcccgtg cggtggagtt
    34681 aggttcctgg ggcgtggatc tcaattgcgg ctgcccgtcg aaaacggtta acggtagcgg
    34741 cggcggggcg acgttactca aagatcctga actcatctac cagggggcaa aagcgatgcg
    34801 tgaagctgta ccggcgcatt tgcctgtcag cgtgaaagtg cgtctgggct gggacagcgg
    34861 tgagaagaaa tttgaaatcg ccgatgcggt tcaacaggct ggcgctacgg agctggtggt
    34921 gcatgggcgg acgaaagagc agggttaccg cgcggagcat attgactggc aggcgattgg
    34981 cgagattcgc cagcggctga atattccggt gattgccaac ggtgaaatct gggactggca
    35041 gagcgcgcaa caatgcatgg cgatcagcgg ctgcgacgca gtgatgattg gtcgcggggc
    35101 gctcaatatt cccaacctga gccgggtggt aaaatataac gaaccgcgaa tgccgtggcc
    35161 ggaggtggtt gctttgctgc aaaaatatac ccgtctggaa aagcagggcg ataccgggtt
    35221 atatcacgtt gcgcggatta aacagtggtt gagttatttg cgtaaagaat acgatgaagc
    35281 aacggaatta tttcagcatg ttcg
//
LOCUS       DAJVQH010000026        34363 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJVQH010000026 DAJVQH010000000
VERSION     DAJVQH010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.026"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..156
                     /locus_tag="OQM87_001388"
     CDS             <1..156
                     /locus_tag="OQM87_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1507579.1"
                     /translation="RCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPT
                     EMIQATA"
     gene            complement(200..4817)
                     /locus_tag="OQM87_001389"
                     /pseudo
     CDS             complement(200..4817)
                     /locus_tag="OQM87_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(4910..5557)
                     /gene="rnt"
                     /locus_tag="OQM87_001390"
     CDS             complement(4910..5557)
                     /gene="rnt"
                     /locus_tag="OQM87_001390"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCS1507580.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5660..6067)
                     /gene="gloA"
                     /locus_tag="OQM87_001391"
     CDS             complement(5660..6067)
                     /gene="gloA"
                     /locus_tag="OQM87_001391"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCS1507581.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6148..7245)
                     /gene="nemA"
                     /locus_tag="OQM87_001392"
     CDS             complement(6148..7245)
                     /gene="nemA"
                     /locus_tag="OQM87_001392"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCS1507582.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7282..7881)
                     /gene="nemR"
                     /locus_tag="OQM87_001393"
     CDS             complement(7282..7881)
                     /gene="nemR"
                     /locus_tag="OQM87_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCS1507583.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            7984..8223
                     /locus_tag="OQM87_001394"
     CDS             7984..8223
                     /locus_tag="OQM87_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCS1507584.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8272..9168
                     /locus_tag="OQM87_001395"
     CDS             8272..9168
                     /locus_tag="OQM87_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCS1507585.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9249..9578
                     /locus_tag="OQM87_001396"
                     /pseudo
     CDS             9249..9578
                     /locus_tag="OQM87_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
     gene            complement(9549..9932)
                     /locus_tag="OQM87_001397"
                     /pseudo
     CDS             complement(9549..9932)
                     /locus_tag="OQM87_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334014.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(9925..10716)
                     /locus_tag="OQM87_001398"
                     /pseudo
     CDS             complement(9925..10716)
                     /locus_tag="OQM87_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(10719..10955)
                     /locus_tag="OQM87_001399"
     CDS             complement(10719..10955)
                     /locus_tag="OQM87_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCS1507586.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            11156..11590
                     /gene="slyA"
                     /locus_tag="OQM87_001400"
     CDS             11156..11590
                     /gene="slyA"
                     /locus_tag="OQM87_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCS1507587.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(11637..12104)
                     /gene="slyB"
                     /locus_tag="OQM87_001401"
     CDS             complement(11637..12104)
                     /gene="slyB"
                     /locus_tag="OQM87_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCS1507588.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            12378..13487
                     /gene="anmK"
                     /locus_tag="OQM87_001402"
     CDS             12378..13487
                     /gene="anmK"
                     /locus_tag="OQM87_001402"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCS1507589.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            13585..13914
                     /gene="mliC"
                     /locus_tag="OQM87_001403"
     CDS             13585..13914
                     /gene="mliC"
                     /locus_tag="OQM87_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCS1507590.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            13973..14629
                     /gene="pdxH"
                     /locus_tag="OQM87_001404"
     CDS             13973..14629
                     /gene="pdxH"
                     /locus_tag="OQM87_001404"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCS1507591.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            14758..16032
                     /gene="tyrS"
                     /locus_tag="OQM87_001405"
     CDS             14758..16032
                     /gene="tyrS"
                     /locus_tag="OQM87_001405"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCS1507592.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            16091..16960
                     /gene="pdxY"
                     /locus_tag="OQM87_001406"
     CDS             16091..16960
                     /gene="pdxY"
                     /locus_tag="OQM87_001406"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCS1507593.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQV
                     KAANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAV
                     NNVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGM
                     RQPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDR
                     IAKPEHYFSATKL"
     gene            complement(17004..17609)
                     /gene="gstA"
                     /locus_tag="OQM87_001407"
     CDS             complement(17004..17609)
                     /gene="gstA"
                     /locus_tag="OQM87_001407"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS1507594.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(17715..19217)
                     /gene="dtpA"
                     /locus_tag="OQM87_001408"
     CDS             complement(17715..19217)
                     /gene="dtpA"
                     /locus_tag="OQM87_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCS1507595.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(19828..20463)
                     /gene="nth"
                     /locus_tag="OQM87_001409"
     CDS             complement(19828..20463)
                     /gene="nth"
                     /locus_tag="OQM87_001409"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCS1507596.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(20463..21158)
                     /gene="rsxE"
                     /locus_tag="OQM87_001410"
     CDS             complement(20463..21158)
                     /gene="rsxE"
                     /locus_tag="OQM87_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCS1507597.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(21162..21782)
                     /gene="rsxG"
                     /locus_tag="OQM87_001411"
     CDS             complement(21162..21782)
                     /gene="rsxG"
                     /locus_tag="OQM87_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCS1507598.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(21786..22844)
                     /gene="rsxD"
                     /locus_tag="OQM87_001412"
     CDS             complement(21786..22844)
                     /gene="rsxD"
                     /locus_tag="OQM87_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCS1507599.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(22845..24875)
                     /gene="rsxC"
                     /locus_tag="OQM87_001413"
     CDS             complement(22845..24875)
                     /gene="rsxC"
                     /locus_tag="OQM87_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCS1507600.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(24868..25446)
                     /gene="rsxB"
                     /locus_tag="OQM87_001414"
     CDS             complement(24868..25446)
                     /gene="rsxB"
                     /locus_tag="OQM87_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCS1507601.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(25446..26027)
                     /gene="rsxA"
                     /locus_tag="OQM87_001415"
     CDS             complement(25446..26027)
                     /gene="rsxA"
                     /locus_tag="OQM87_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCS1507602.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFATIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(26104..26544)
                     /locus_tag="OQM87_001416"
     CDS             complement(26104..26544)
                     /locus_tag="OQM87_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCS1507603.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(26630..26845)
                     /gene="ydgT"
                     /locus_tag="OQM87_001417"
     CDS             complement(26630..26845)
                     /gene="ydgT"
                     /locus_tag="OQM87_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCS1507604.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(27118..27243)
                     /gene="blr"
                     /locus_tag="OQM87_001418"
     CDS             complement(27118..27243)
                     /gene="blr"
                     /locus_tag="OQM87_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCS1507605.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            27486..28526
                     /locus_tag="OQM87_001419"
     CDS             27486..28526
                     /locus_tag="OQM87_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1507606.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(28562..29563)
                     /gene="add"
                     /locus_tag="OQM87_001420"
     CDS             complement(28562..29563)
                     /gene="add"
                     /locus_tag="OQM87_001420"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCS1507607.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(29666..30838)
                     /gene="malY"
                     /locus_tag="OQM87_001421"
     CDS             complement(29666..30838)
                     /gene="malY"
                     /locus_tag="OQM87_001421"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCS1507608.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(30848..32440)
                     /gene="malX"
                     /locus_tag="OQM87_001422"
     CDS             complement(30848..32440)
                     /gene="malX"
                     /locus_tag="OQM87_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCS1507609.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            32615..33643
                     /gene="malI"
                     /locus_tag="OQM87_001423"
     CDS             32615..33643
                     /gene="malI"
                     /locus_tag="OQM87_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCS1507610.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            33755..>34363
                     /gene="hdhA"
                     /locus_tag="OQM87_001424"
     CDS             33755..>34363
                     /gene="hdhA"
                     /locus_tag="OQM87_001424"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCS1507611.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFSWRCDITSEQELSALADFAVSKLGKVDILVNNVGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITP"
BASE COUNT         8570 a         8811 c         8924 g         8058 t
ORIGIN      
        1 agatgccagg tatgtggata tacagcgaac gccgatgtaa atggcgctcg taacatttta
       61 gcggcggggc acgccgttct tgcctgtgga gagatggtgc agtcaggccg ctcgttgaag
      121 caggaaccca ccgaaatgat tcaggcgaca gcttgaacgt agcagggaac cacgtctttc
      181 agggcgtgga ggatgtcaac tatccccaat ccagcccttg tggcgaactt gaaaatcccg
      241 cctcacgcag cagcgtaaac atcggcgttt gccggaccgg aagatcattc acttctgtta
      301 gcgtaaagcg cagccttggt tcgcgacgca gtgcggtggt cagcgcctgg aaaatctctg
      361 gggcgagtaa ttcctccttt tcctgccaga ccagcatttt tttgccgcct tgcggcagat
      421 agagtaacaa tttgccgcca gaaacgacca ccagcgcacc cgcccgacgc gttggtacca
      481 gcgctgccgg atgtgcaggc cagggcagaa gatttcccca cacattagcc ggatcgttgg
      541 cggagagcgc cactggtgta tagtggcgtg tttgcgtggc ttgtgtcgcc agatcgcgca
      601 atcggtcaat agtcagacgt tcagcgaatt gcgcgccacc cagaccttct acaaaacgac
      661 ctcgcataat tcgcccggag tcttccatac ttcgacaaag tgtttgcatc gatggaaacc
      721 cgccagggat attttcggct atcaccgcct gacgactgat gatgccgtag cggtcgagca
      781 tattttccgc cagcgccagc atcctttcgg tatcgtttag tggctccacc tgcaataacg
      841 accagcgtcc agccagattt ggtgtgttgt aagatacccg cggcgagacg ggttgtgcat
      901 agacaggacg cccacgacga gcccggtgac tgcggcgagt tgaggtgcgt gcgttggaac
      961 tgctgcgggt gagggcgcgt aacggtgccc aaatatcgct ggtaatgacg ccttgccaga
     1021 ccagcgccca taacgcctct tgcagtgccg aggaatcaac cgattcgccg attttatcgc
     1081 gtatccgttg gctgatttgt tgtgcaaacc aggctcctcc gtcagccaga acagcgatta
     1141 tcgcttgctg cagcgccgaa cgattcgcct gatccgcttc ggcgggagtg aacgattctg
     1201 cggcatattt ctgtagatgc agtgccacca ggccgtcatc ttcacccagc ttttttttgt
     1261 cccgaccaga taaccgctcc ggttgccagt aattcatcga gcatttctgg cgaatagtcg
     1321 cgtacgcggg caggcaggat ctggctttcc cagagtgagg cgggtaaacc gactccggca
     1381 agctgttcga tcacccgcat cacgccatct acgccctcat aaacgcctgg cgatgttgag
     1441 gcaaagagcg ccgggctacc atcggtggcg ggtaatacgc cctgacgttc cagcagcaat
     1501 cgcgcatagg tcgtggctgc aacgggacgc gtcgcttctc tggcggcttg cagcgagcgc
     1561 aaacgcagac gacgaaatac ttcatcgctc acccagatgt cttgttgcag attcatcacc
     1621 agaccctgtt cacgcagttg ctgaagctgc tcttcgacaa tggcaatacc gagactaaac
     1681 tcatgagcca gttgttcagc cgtgaccaga gcatgagccc ggagatagcg cagaaagagg
     1741 tcgcgaagcg ggtaactgac tctatgtaaa taaatctctg gcaatgactc tggtagtcgt
     1801 acgccgaggg catcacgcag cctggcggca tcatccatac aggcaagacg ctcctgtccg
     1861 ctaatcatcg cagggaagat tcgttttact gcaagaagat tttccagata cgacgcaacc
     1921 tcttcactgc tgcctgtatg ccgttgcgcc aggtcttcaa cggtcattgg ccccagttcg
     1981 cgcagcaggt cgaacaatcc ttcttcacct ttcgctcttc tgccaggagc cagtcgttgc
     2041 aactcttctt ccacctggcg aatgacctgc gggtcgagta attcccccgg atcgacctgt
     2101 ccgagtagat tgcgcagtaa ctcgctgtcc agcgacagta cggatgcccg gcgctctgcc
     2161 agcggggcgt cgctctggta cataaattcc gcgacatagc cgaacaataa acttgtggcg
     2221 aaaggcgagg gcgtagtggt cgttatatcg gatatttgaa tttcgccacc gttcaggcga
     2281 cgcatcaaac gttccagtgc gggaagatca taaacatctt gcagacattc gcgtagggtt
     2341 tcgagaatga ccggaaaatc cggatatccc tgagcgattt ccagcaactg actggcgcgc
     2401 agccgttgtt gccaaagcgg ggtgcgatgg cccggcgtgc ggcccggcat taataatgcc
     2461 cgcgcggcgc attcgcgaaa acgggcggcg aaaagtgccg agctgcctac cgcctcgcgg
     2521 acaatttgca gcaacttttc tggttcaaac aaaaaaatcg cggcgtcggg caatttacca
     2581 tcagtgtcag gaatgcgcgc aacaatgccg tcatcactgg cgaccacaga cgcgtcagcg
     2641 ccccataggg catgtattcg cccggcaatc gccagcgccc agggttcatg cacccgtctt
     2701 ccatagggag agtgcaaaat aatacgccag tcgccaattt catcacggca ccgttcgagg
     2761 accagatggc gactccccgg aacgatgccc gtcgcgttgc gttgctcgtc aatcaacccc
     2821 tgaatattgg cgttcgtatt ttcttctgcc agccacgggg gaatagtccc ggaaaagaac
     2881 gcgccatccg ccagtaaatg aagaaaatcg ccgatcatct cgcctaattc agccggacgt
     2941 ccgttacctt caccacgcca gaaggggagc cgggcagaac gacccggagc aggagtcaca
     3001 atcacctgat cgcgggtgat ttgctggatc cgccatgagg tagcgccgag agtgataatg
     3061 tcgttcaccc gcgactcata taccatctcc tcatccagtt cacccacccg ccgcgaaccg
     3121 gccttttctt caccttcggg taataacacg ctatacatgc cacgatccgg aatggtgccg
     3181 ccgctggtaa ccgccaacaa ttgagcgcca ggtcgggcgg tcaatatccc ggtctccctg
     3241 ttccagacca gtttggggcg aaaagcagaa aaatcgccag agggatagcg cccggaaagc
     3301 atatccagcg tggcgtcaaa aacacgtctt ggcagatctt tccacggtgc ggcacggcgt
     3361 acgcgggagt accattcgtc tacctgtaat gcatccatcg ccgcggcggc aacggtttgc
     3421 tgcgcaagga cgtcgagagg attatgcggt ggtgtcaggt tttccagcct gcctgcgaac
     3481 atacactcta caatgactgc ggaatcgact aaatcacgcc gggtacgggg gaaaaacagc
     3541 cctttagata caccgccaac ctgatgtccg gcgcgaccaa tgcgttgtaa cccactggca
     3601 acagaaagcg gcgttgccac ctgaatcacc agatccaccg cgcccatatc aatccccagt
     3661 tcaagactgg aggttgcgac cacgcagcgt aattcacccg atttcagcgc ctgttcggtg
     3721 attgctcgtt gttctttaga gacggagccg tggtgtgagc gggcaataaa aacgtcgcta
     3781 ctttgtacac ggttagaggt tgcgccggag gtcgactcga aatgggccgc atcaacggcg
     3841 atagacgggg aacgctgtaa gcgtgcggcg taaagctcat ttaatcgtgc cgtcagtttt
     3901 tccgccagcc cgcgtgaatt agtaaagaca atggtcgagc gatggcgcaa cacttcatca
     3961 aggatacccg tttcaatata tggccagatg gagccttccc ggccggcatg gctgtcttcg
     4021 ccggtgccgc tggcgaccga tgagacatca tccatattgg cgaccggtac gacaattcgt
     4081 atctgcggat ggcgcattgc gggcgggttg actaccgtaa ccgggcgatc gccaccaaga
     4141 aatgctgcca catcgctggc tgagcgcaca gtggcagaaa ggccaattcg ctgtgctgag
     4201 gtgtggagca gcgcatcgag ccgctccaga cttaacgcca gatgcgcacc acgtttactg
     4261 cccgctaccg cgtggacttc atcaataatt accgtttcga cgccgtgtag cgtttcgcgc
     4321 gcgcgggagg tcagcatcag atagagtgat tcgggtgtgg tgatcagaat atccggcgga
     4381 ttacgggtga gtttgctgcg ttcctgtgca ggcgtatcgc cagtacggat ccctacgcga
     4441 agattgactt ccgtttcgcc gcgccgccgc cgttcatcgg caataccctt caacaggatc
     4501 tgcaagttgc gctgaacgtc ggtgcccagg gcttttatcg gtgaaatata gaggatgcgt
     4561 gaggttttac gcttatgcgc ctcgcgggta tcttcgccgc cttcgcggaa gagccgatcg
     4621 agggcgtaga gaaatgctgc cagcgttttc ccggagccag tcggtgcaat caccagcgca
     4681 tgttcgcctc gcgccgccac atgccaggtt tgcgactgga cagcggtcgg ctgtttaaag
     4741 gcgcgaagaa accagtcgcg ggtcgccggt gaaaacacgt ccggcaggag cgatgaaggg
     4801 tctggattat ctgccattaa cgctattccc cgtgaatatc atgcttgatg cgtggaatag
     4861 cctcactata gatgcctgaa tcattgcatg tcatcaggca tcgactcgat tacacctctt
     4921 cggcggcagg tagcggccag cctcccagac gtttccagcg gttgacgatt tcacaaaaca
     4981 gcacagcagt gcgttcggtg tcgtacagcg cggagtgcgc ctgggtgctg tcgaagtcca
     5041 tgccagcggt ctggcaagcc tttgacaata cggtttgtcc gagtgccagc ccggccagtg
     5101 cagcagtgtc aaaagtggcg aaagggtgga acgggttacg tttcagcgag gcgcgttctg
     5161 cggcggccat cataaagctg tgatcaaaat tggcgttgtg cgccaccata atggcgcggt
     5221 tacagccgct cgctttaata cctttacgta caactttaaa aatttcgtgc agcgcctcgt
     5281 attcgctgac cgcgccgcga tcgggatcgt tcgggtcaat gccgttgaag gcgagggctt
     5341 ctggttgcaa atttgcgccg acaaatggtt cgacgtggaa atgtaatgtg gtgtccggca
     5401 tcagccagcc ttgttcatcc attttcaggg tgatggcggc aatctcaagc agcgcatcgg
     5461 ttttggcgtt aaatccggct gtttcaacat cgatcacaac aggataaaaa ccacgaaaac
     5521 ggtcgcacag accggtaagt tgagcgttat cggacatctg attctcttaa tacggataaa
     5581 ttgcagcgcg cattatgaca aatattactt gtcgatgcag taaagatgcg ggcgcgatga
     5641 gttcacgccc ggcaggagat tagttgccca gaccgcgacc ggcgtctttc tcttcgatta
     5701 actcaatttt gtaaccgtcc ggatcttcca caaacgcgat aaccgtagta ccgcctttta
     5761 ccggacccgc ctcacgggtc acgttacccc cgttttgacg gattttttcg cacgcttcag
     5821 cggcgttatc tacgctaagc gcgatgtgac cataagcagt gccgagttcg tatttatcca
     5881 cgccccagtt gtaggtcagt tcaatcaccg cttcttcggt ttccgggccg tagccaacaa
     5941 acgccagtga gtatttgtat tccgggtttt cgctggtacg cagcagtttc atgcccagca
     6001 ctttggtata aaaatcgatg gagcgttgca aatcgccaac gcgcagcatg gtatgaagaa
     6061 gacgcatctt tttatcctca aaatggctaa cagattcaaa atgttgtttt agtatagcgg
     6121 cggctttacg ccgctcgcaa tgttggatta caacgtcggg taatcggtat agccttccgc
     6181 gccgccaccg tagaaacttt cggcacgctg tgggttaagc tcagctttga gctgcaagcg
     6241 ggcgaccaga tccgggttcg caatccagtc acgaccaaat gccaccgcat caattaaccc
     6301 tttgccgatc agcgtttcag ctttttctac tgtgtatgca cctgcgccga taatcggacc
     6361 gtggaaacgg gcgcgtactt tttcgcggaa cgcatcagta tacggttcac cccccgccca
     6421 atctggttct gacatatgca gataagcaat gccgcgttta cccagttgtt caatcagata
     6481 cagtgcatcg gcttcttcat tcgggccgtt atctgtgttc tggaaagtac cgattggcga
     6541 aacgcgaatg ccaatgcgat cggcacccca ttcttcaatc ccggcatcga ccacttccag
     6601 taccaaacgt gcgcgatttt ccacgctgcc gccgtactga tcggtacgat ggtttgaaga
     6661 aggggaaagg aactgatgca gcaaataacc gtgagcagag tggagctcta ccagatcaaa
     6721 accggcttca cgcgcgttag caatggcctg acggaaatca ttgacgatac ccggaatctc
     6781 ttccagttca agcgcacgcg gcatggatgt ttcaacgcgg atcgcccgac cattttcatc
     6841 gcgcagagaa gtacgtgttc ccgcgctaag tgctgaaggc gctaccggtg cctgaccgcc
     6901 aggttgcagg ctggcgtgag aaatgcgtcc ggtgtgccac agctgcaagg ccatatgacc
     6961 attttcagca tgaacgccag cggtgatttt tttccatgcg gcaatttgct ccggactatg
     7021 gatgccaggc gcacctgcat atccttttgc ctgggcagaa atttgcgtgg cttcactaat
     7081 aatcaaaccg gcactggcac gttggcgata gtattccgcc atcaacgggg taggaatgtc
     7141 acccggttca atactgcgca gacgcgtcag cggtgccata aaaatacggt ttgccgccgt
     7201 gatcgcgccc actttcagtg gggaatacag tttttcagat gacataaagg ctcccggtcg
     7261 tctggtgata aaaaatgcct gctaaacggc aggcgtcgca ataatgtttt ttacatgggc
     7321 cagcgcgttt tccagtggct cgaaactgcg cgaaattttg gcctgcagat tcgcgccaag
     7381 ccatagtgcg taaagcactt gtgcctgttg tagcggttcg ccacaaaagg ttaaacaatg
     7441 gttctcacgg ccattttcca gagcctgcga gagcagggcg atcacgccgc gagcaccttt
     7501 atccatcgcg ctgcgcatat cttctgacag atcgcacact tcggcagaga gttttactgt
     7561 caggcaacca ctgatggttc catgttggca aaactggttc agtgtttgct ggtaataagc
     7621 cagtatgcgg tcgcggtagt taccttcgcc ggattgcagc aactcagcca gtcgctggtg
     7681 atatgcggcg taatgacgct caagcatggc aacgccaaac gcttctttag agcgaaagta
     7741 gtgatagaag gaccctttcg gcacttcagc ggtttttagt aattcgctta gccccatccc
     7801 ggtgaatcca cgttgcaggc aaagttgctc gcccgtcgcc aagagatgtt cgcgagtatc
     7861 atgttcggtg tgtttgttca tgcgttggag tgtagtagac cagtcggtct agtgcaagca
     7921 aagttgctac tttggacatt aacagcttca ataatggtaa taatcttatc tgaggagggc
     7981 gaggtggcgg agcaattaga gttctttccc gtccagagcc cgtgccgggg aatttgccag
     8041 tctgacgaac gcgggttttg tcgcggctgt tttcgtagtc gggatgagcg ttttaactgg
     8101 aataaaatga gcgatggcga aaaacaagag gtgctgcgcc tttgtcggca gcgattaatg
     8161 cgcaaattac gcgcaaataa gccagcgtca tcagacgaac cggagcaacc atcactcttt
     8221 tgaacctaat cttgcgtata ctcctctcat atctgataag aggaagcgat tatggttcag
     8281 cgtattacta ttgcgccgga aggcccggag ttttcccgtt ttgtgatggg ctactggcga
     8341 ttgatggact ggaatatgtc cgcccgccag ctggtcagtt ttattgaaga gcatctggat
     8401 ctcggcgtga ccaccgtgga ccatgctgat atttatggtg gctatcagtg cgaagcggcg
     8461 tttggcgagg cactgaaact ggcacctcac ctgcgtgaac ggatggaaat cgtcagtaaa
     8521 tgcggtatcg cgacgaccgc gcgtgaagaa aacgtcattg gtcattacat cactgaccgc
     8581 aatcacatca ttaagagcgc cgaacagtcg ctaattaatc tcgcgaccga tcatctggat
     8641 ctgctgttaa tccaccgacc agacccgtta atggatgccg atgaagtggc ggacgcgttc
     8701 aaacatctgc atcagagcgg caaagtgcgt cattttggcg tatcgaactt tacgcctgcg
     8761 caatttgccc tgttgcaatc acgtctgccg tttacccttg ccactaatca ggtggaaata
     8821 tccccggtgc atcagccgtt actgctggat ggcacgctcg accaactaca acaactgcgt
     8881 gttcgtccga tggcgtggtc ctgccttggt ggtggtcgtc tgtttaatga tgattatttc
     8941 cagccgctgc gtgatgaact ggctgtggtg gcggaggagt taaacgcggg ctcgattgaa
     9001 caggtggttt acgcctgggt attacgttta ccgtcgcagc cgctgccaat tatcggctca
     9061 ggtaaaattg agcgcgtacg ggcagctgtc gaagcagaaa cactgaaaat gacccgtcaa
     9121 caatggtttc gtatccgtaa agctgcactg gggtacgacg taccgtaagc acttttagga
     9181 atagccgccg ttcaaaaatg tgtcactggt ttacacttat tcaggaatgc acaatgaacg
     9241 gaggtcctat gaaacgtttt agtctggcca ttctggcgct ggttgttgcg accggcgcac
     9301 aagctgccag tgaaaaagtc gagatgaacc tcgtcacgtc gcaaggggtt gggcagtcaa
     9361 ttggtagcgt caccattact gaaactgata aaggtctgga gttttcaccc gatctgaaag
     9421 cattaccccc cggtgaacat ggcttccata ttcatgccaa aggaagctgc cagccagcca
     9481 ccaaagatgg caaagccagc gccgcggaat ccgcaggcgg gcatcttgat ccacaaaata
     9541 ccggtaaaat taaccatcgg aaaggcgatg atggcagccg tgtagcccgc cagttgaaat
     9601 gcatacgcga cgttattcgt gaagtgcgca caggcctagg tacaaaagcc aagccacgcc
     9661 gacatgctca atagaaaaaa ccacggctca ttgagcgtat gtccggcaag aagcagtgcc
     9721 gcaatggctc ccagtaaact gccagcgatg cgtccgaggc ttttgctgat gacaccgcca
     9781 acggtgggaa agctaaccac tgcagccgag gtcatcgccc aatagggttc atccagattc
     9841 aaataatagg caaccgtcag cgccagacac atggcaatgg tattgcgtaa cgcataacgc
     9901 cattgcgcca gcgtggccct gaaccagcgc aaagcggacg ggaacgcgtt gggcgagacg
     9961 aacccagggg acgttaggtt tcacatccgg aataaggctt gagtcacttt caacgctctg
    10021 atcataaatc gcccgaccga tactggaaac gtgaccctgt aacgttttgt tatcactata
    10081 gagggtaatt tgtgctggtg caccttcgcg gatatggcgc aacttggttt cttcaaaata
    10141 accaatgacg taaaacgagt ggctgtcgac aagggcaaac agcggtttcc cggtgtcagc
    10201 gtaatcgccg atgcgggtag tgaggttagt cacccatcca ctcaccgggg cgcgaatttc
    10261 cgtttgcgcc agttgccatt gtgtctgttt cagcgtcgcc tgtgcggcat taacactggc
    10321 ctgcatcgct ttaacattga ggttagcggt atcgagttct tcggcaaaaa taaaattttg
    10381 tgagagatga cgacgacgat tcgcttcgtt attggctttt gcgaggtcag attgcgcttt
    10441 cgccagctga gcctgtgcgt tcagctcggc gatctgaaag ggcgttttat cgatcgtgag
    10501 cagaagttcc cccgcgttaa ccagttggtt gtcttttata ttcagctcaa caatacgtcc
    10561 cgacacctgt ggggtgatag aaacctgttc tgcgtgtatt ttcccgtcgc gtgtccacgg
    10621 agattgcatg taataattcc acagccacca tccggcaaga accgcaacta tcgctacaat
    10681 gattgttgag aaatacttaa ttgttttcat tgacatagtt accacgcaat cagtattgcc
    10741 agagcaaggc aaacgcaaat cgcaaacagc gataaatcca ttaacaaggg atgccagatg
    10801 tcaccggcgt agatccagcc acgaagcaga cggtgtacga caagccagat gacgaatcca
    10861 aacgcgaatg ctttgaaaaa cggaggaaaa tagacggacg caccgaacac cagatcttgt
    10921 aagggcaatc ctgttgcatt gagcataaac ttcacaaagt agattccttt acgaccgaat
    10981 atattgcgtt agattaataa atattcttta agtgcgaaaa atttacgcgc aatttctgaa
    11041 aaatatgtct gacggtaacc aaatgcagca atacatttgt tttagcaata caattgctgc
    11101 acactattct aaaagccgca taatatctta gcaagctaat tataaggaga tgaaattgga
    11161 atcgccacta ggttctgatc tggcacggtt ggtgcgcata tggcgtgctc tgatagacca
    11221 tcgcctgaaa ccgctggagt taacacaaac ccattgggtt acgttacaca atatccatca
    11281 gttacctcca gaccagtcgc aaattcaact ggcaaaagcg attggcatcg agcagccatc
    11341 actggtccgt actctggacc aactggaaga aaaagggtta atttcgcgtc aaacttgtgc
    11401 cagcgatcgt cgggctaaac gtattaaact gacggaaaag gcagagccac tgatcagcga
    11461 aatggaagct gttattaaca aaacccgcgc ggaaatatta catggcatct ccgcagagga
    11521 actggagcaa ctgattacgc tcatcgcaaa acttgagcat aatatcattg agttacaggc
    11581 caaagggtga aatgaagggg gcgtgtggtc agtaacctga ccacacgcaa acttatttag
    11641 cgcggagaaa cggtcacctg actgccattg ctggccagta cgacacgttg gcccggagag
    11701 aaacgagtgt tgccttgttt ctgtaccacc atgatggtat taccatcgtc tttacgaatt
    11761 tccagctcga caccctgcgt tttgttcatt gcactctgta cgccctgacc agctacgcca
    11821 cctgcaacag cgcctgctgc agtagccaga gaacgcccgg ttccgccacc aacagtattc
    11881 cccaggaaac caccaagaac agcaccgcca attgcaccga taacgttgga atcatcaccg
    11941 ccctgaatct gtaccggacg tacgttaacg atggtgccat agctgacatt ctgtacttgt
    12001 ttcgcttcag aagcggtata aacatcccct gacagggtgt cgttattaac acaaccgaca
    12061 agagacagac ctaccattga aacaaccaat acgcgtttaa tcactgaaaa atctcctgtt
    12121 caccatgaaa cgccacgcaa gcatccctca tggtcaaagt atatggcata tgtgaatcca
    12181 tggttacatg ttctaccaaa tatgagcaaa tcataatgaa agtaggatca accaattata
    12241 aacaaaacat tcatgatgat tattgatttg aagataatgt tttactacca acggtaaaaa
    12301 tcggtcattc cgcatggtat tcacgaaaag tttatgtttg agtggtgcgc aaacgtcaga
    12361 ttgaaaaagg atgaactatg aaatcgggcc gctttattgg cgttatgtca ggcaccagcc
    12421 ttgatggtgt tgatgttgtg ttggcgacaa ttgatgaaca ccgggtcgca cagctggcaa
    12481 gcttgagctg gccgatcccg gtgtctctga aacaggctgt actggatatt tgccagggcc
    12541 agcagcttac actttcgcag tttggacagc ttgatactca actcgggcga ctttttgctg
    12601 atgcggtcaa tgccttgctt aaggaacaaa acctgcaggc gagagatata gttgcgatcg
    12661 gctgtcacgg tcaaaccgtc tggcatgaac cgacgggcgt ggcaccacac actttacaga
    12721 ttggcgataa caatcaaatt gtggcacgca ccggaattac ggttgtcggt gattttcgcc
    12781 gtcgcgatat tgccttggga ggacaaggcg caccgctggt acctgcgttc catcatgcct
    12841 tgctggctca cccaaccgag cgacgaatgg tgctcaatat tggcggcatc gccaatctgt
    12901 cactgctcat tcctgggcag ccggttggcg gctacgatac cggtcctggt aacatgctga
    12961 tggatgcctg gatctggcgt caggcaggta aaccttacga taaagatgcc gagtgggcac
    13021 gggcgggtaa agttattctc ccactgctgc aaaatatgct cagcgacccg tatttctcgc
    13081 aacctgcacc gaaaagcacc ggacgcgaat actttaacta cggctggctg gagcgccatt
    13141 tacgccattt tccgggtgtt gatccccgtg atgttcaggc cacactggca gaactcaccg
    13201 ccgtgaccat ttctgaacaa gttttgttga gcggtggctg cgaacgattg atggtatgtg
    13261 gtggaggtag tcgtaatccg ctactcatgg cgcgtctggc ggtattactg ctaggcacag
    13321 aagtcaccac caccgatgcc gttggcatta gtggcgatga catggaagca ttggctttcg
    13381 cctggcttgc ctggcggacg ctggcgggat taccaggaaa tctgccttcc gtcactggcg
    13441 caagccagga gacggtactg ggggctattt tccccgccaa ctcgtgatat cagaattaac
    13501 tgaatttttt cttatcgtgt ctgccgttac acttggaaga ttaaggaggg tatttgccct
    13561 ccggaccagg aaagtcttcg ggatatgact atgaaaaaac tgttaattat cattttgccg
    13621 gtccttctct ccggttgtag cgcctttaat caactggttg agcgaatgca aaccgatacg
    13681 ctggaatacc agtgtgatga aaaaccgttg acggtcaaac tgaataatcc gcgccaggaa
    13741 gtcagttttg tttacgataa tcaactactg catctcaaac agggcctttc agcctctggc
    13801 gcgcgttact ccgacgggat ctatgttttc tggtcgaaag gtgaggaagc gactgtctat
    13861 aaacgcgacc gcatcgtctt gaataactgt cagttacaaa atccacagcg ttgagatttt
    13921 tccaggggcg gcgcacaata gcgtcaccca ctgacaatcc gtaaagaaaa ccatgtctga
    13981 taacgacgaa ttgcagcaaa tcgcgcatct gcgccgtgaa tacaccaaag gcgggttacg
    14041 ccgccgcgat cttcccgccg atccattaac cctttttgaa cgttggctct ctcaggcttg
    14101 tgaagccaaa ctggcggacc ctaccgcgat ggtggtcgct accgtggatg aacatggtca
    14161 gccttatcag cgcatcgttt tactcaaaca ttacgacgaa aaaggcatgg tgttttacac
    14221 caacctcggt agccgtaaag cacatcaaat tgaaaataat ccgcgcgtta gcctgctgtt
    14281 cccgtggcat acccttgaac gccaggttat ggtgatcggt aaagcagaac gactttcgac
    14341 tctcgaagtg atgaaatatt ttcatagccg cccgcgtgat agccagattg gtgcatgggt
    14401 ttcgaagcag tccagtcgca tttctgcccg cggtatcctt gaaagtaaat tcctggagct
    14461 gaagcagaag tttcaacagg gcgaagtgcc attgccgagc ttttggggcg gttttcgcgt
    14521 cagccttgaa cagattgagt tctggcaggg cggtgagcat cgcctgcatg accgcttttt
    14581 gtaccagcgt gaaaatgatg cgtggaagat tgatcgtctt gcaccctgaa aagatgcaaa
    14641 aatcttgctt taatcgctgg tactcctgat tctggcactt tattctatgt ctctttcgca
    14701 tctggcgaaa agtcgtgtac cggcaaaggt gcagtcgtta tatacatgga gattttgatg
    14761 gcaagcagta acttgattaa acaattgcaa gagcgggggc tggtagccca ggtgacggac
    14821 gaggaagcgt tagcagagcg actggcgcaa ggcccgatcg cgctctattg cggcttcgat
    14881 cctaccgctg acagcttgca tttggggcat cttgttccat tgttatgcct gaaacgcttc
    14941 cagcaggcgg gccacaagcc ggttgcgctg gtaggcggcg cgacgggtct gattggcgac
    15001 ccgagcttca aagctgccga gcgtaagctg aacaccgaag aaactgttca ggagtgggtg
    15061 gacaaaatcc gtaagcaggt tgccccgttc ctcgatttcg actgtggaga aaactctgct
    15121 atcgcggcga acaactatga ctggttcggc aatatgaatg tgctgacctt cctgcgcgat
    15181 attggcaaac acttctccgt taaccagatg atcaacaaag aagcggttaa gcagcgtctc
    15241 aaccgtgaag atcaggggat ttcgttcact gagttttcct acaacctgtt gcagggttat
    15301 gacttcgcct gtctgaacaa acagtacgat gtggtgctgc aaattggtgg ttctgaccag
    15361 tggggtaaca tcacttctgg tatcgacctg acccgtcgtc tgcatcagaa tcaggtgttt
    15421 ggcctgaccg ttccgctgat caccaaagca gacggcacca aatttggtaa aactgaaggc
    15481 ggcgcagtct ggttggatcc gaagaaaacc agcccgtaca aattctacca gttctggatc
    15541 aacactgcgg atgccgacgt ttaccgcttc ctgaagttct tcacctttat gagcattgaa
    15601 gagatcaacg ccctagaaga agaagataaa aacagcggta aagcaccgcg cgcccagtat
    15661 gtactggcgg agcaggtgac tcgtctggtt cacggtgaag aaggtttaca ggcggcaaaa
    15721 cgtattaccg aatgcctgtt cagcggttct ttgagtgcgc tgagtgaagc ggacttcgaa
    15781 cagctggcgc aggacggcgt accgatggtt gagatggaaa agggcgcaga cctgatgcag
    15841 gcgctggtcg attctgaact gcaaccttcc cgtggtcagg cgcgtaaaac tatcgcctcc
    15901 aatgccatca ccattaacgg tgaaaaacag tccgatcctg aatacttctt taaagaagaa
    15961 gatcgtctgt ttggtcgttt taccttactg cgtcgcggca aaaagaatta ctgtctgatt
    16021 tgctggaaat aatgcattaa gtggaaaggg ggagtgagaa atcactcccc ctggttttta
    16081 tacagggaac atgatgaaaa atattctcgc tatccagtct cacgttgttt atggtcatgc
    16141 gggtaacagt gcggcagagt ttccgatgcg ccgcctgggc gcgaacgtct ggccgctgaa
    16201 caccgttcaa ttttctaatc acacccaata cggcaaatgg actggctgcg tgatgccgcc
    16261 cagccattta accgaaattg tgcaaggcat tgccattgcc gccattgata aattacacac
    16321 ctgtgatgcc gtattaagtg gctatctggg atcggcggag cagggtgaac atatcctcgg
    16381 tatcgtccgt caggtgaaag cagcgaatcc gcaggcgaaa tatttttgcg atccggtaat
    16441 gggtcatccg gaaaaaggct gtatcgttgc accgggtgtc gcagagtttc atgtacggca
    16501 cggtttgcct gccagcgata tcattgcgcc gaatctggtt gagctggaaa tactctgtga
    16561 gcatgcggta aataacgtcg aagaagcggt tctggcagcg cgcgaactca ttgagcaagg
    16621 gccacaaatt gtgttggtta aacacctggc gcgagctggc tacagccgtg accgttttga
    16681 aatgctgctg gtcaccgccg atgaagcctg gcatatcagc cgtccactgg tggattttgg
    16741 tatgcgccag ccggtaggtg ttggtgatgt gacgagcggt ttactgctgg tgaaactgct
    16801 tcagggggca acgctgcagg aggccctgga acatgtgacc gctgcagtct acgaaatcat
    16861 ggtgaccacc aaagcaatgc aggaatatga gctgcaagtg gtggctgctc aggatcgtat
    16921 tgccaaacca gaacattact tcagcgcaac aaagctctga aatatctcat ttcggcccac
    16981 aacgacctgt gggccgatgg ctattacttt aagccttccg ctgacagcgc gtcttgtact
    17041 tccggacgtt cagccatacg ttgcataaat gccgcaatgt gctctaaccc ttccagattc
    17101 agtttcaccg cgtatgccca gcgcagaacc gtaaacagat aggcatcagc aattgtaaat
    17161 ctttgcccgc agatccagtg ctcatccttc agtgcctcgt tcacatattg cagcttctta
    17221 tccagctgcg cgcgaactgt cggtttgtac tcttccggtg tatctgggcg aaacagaggt
    17281 gtgaaacctt tatgcagctc ggtggcgatg taattcagcc attcgatggt tttatagcgg
    17341 gaaatactgt ttaccggtgc cagcaactgg cggtcgggga cgctgtcggc aagatactgc
    17401 ataatcgcaa cgccttccgt cagcaaagta ccatcgtcca gcagcaatgc aggcacctgc
    17461 cccttagggt taacggaaaa gtaatcgtca ccgttttcga gacgtttttt cattaaatcc
    17521 acactgacga gcgtaaaatc ctttccgctc tcacgcaggg taatatggga agcgagagag
    17581 caggcgcccg gtttgtagaa caatttcatc ggtaactcct tttgtctgtg gtttaaagta
    17641 tggtagtgcg ttgctgaaca aaaaaaaagc cgctaacgaa aagttagcgg cttgtaaaag
    17701 agtttccctg aattttacgc tacagtagct ttcgccgctt tgtctgcagc gtcatcctgc
    17761 gtcatgcggt gcagtttagg cgcggtcagc agcatcagta ctgcaataac ggcagtagcg
    17821 acaccaatct gcaagaatac gcgaccatag acttccagtg acatcagcgg gtcggtaacg
    17881 ttatccggca cagccatcat acccgcaaca taaccaccaa tcaggtttgc accggcagtg
    17941 gtcaggaacc agctacccat aatgaagccc atcagacgct gcggaacgag ttgtgcaacc
    18001 attgccagac ccagaccaga gatcatcagt tccccgatgc tctgcaggcc atagcttgcg
    18061 accagccagc tgacagacac gatacctgcg tcagacgcga atttcgcacc cagcggcaga
    18121 atcaggaacg caccagaaca catcaccatg ccgattgcaa acttggttgg catcggcagg
    18181 gtatcaccca tcttgttata gatagcggcc agaatcggac taccgatgat gatccagaac
    18241 gggttcagtg cctgatactg ctcaggttct acggccagac ccagaatgga gtgctcaacg
    18301 ttacgaatcg caaagaagtt cagtgacgtt ggcatctggc tgtacagcac gaagaagatg
    18361 attgcttcga gcatcaggat gaaggcaacg atcattttac gacgcgcagc acctttcatc
    18421 gcgaaagctt ctttaccgaa gataaccaca ataccgaagg caacaacgcc cagcgccata
    18481 cgcgcaactt cctgattgtg cagtagccag gtggcaacag cgatcagcgc cacaacacca
    18541 ataatggtca gcaacaggtt acggtagttg actggttcaa agtctggttt tgaaccgtac
    18601 tgtttaaccc agcgttggca gaaggcgaag ttaacgatag tgatcagcag gcctacaacg
    18661 ctcaacgcaa acgcaacact ccagccgtat ttcgcggcca gccacggggt ggctatcata
    18721 gagaagaaag agccgatgtt gacggacatg tagtacatgg tgaatgcacc gtccagacgt
    18781 gggtcgtttt tctcatagca tgtagaaagc agagaagacg ggttagcttt aaacaggccg
    18841 ttaccgaccg caatagccgc catacccata taaacaatac cggcgtcgtg accagaccag
    18901 gcaaccagag cataaccaat cgccagcaca atagcgccga gcataattac gcgtttagta
    18961 cccagtacct tgtcacctaa ccagccgccg atagcgacca gaccataaac cagggcacta
    19021 aaggaagaga aaagggtgat tgaatccgct tcagacatac ccagttgttt aaccaggtaa
    19081 acagccataa ttccttgtag gccgtaataa ccaaaacgtt cccataactc aatcgagaag
    19141 atgagataga acgccttcgg ttgtttgaaa gcgttcaaac tgacgctttc agttggtttt
    19201 tggtttgcag tggacacttt tacctctttt tttacatccc atattaacgg gggtgctttc
    19261 tcgcgttatg tctaaaaaac atacgcgcaa ttgttatagt gggaggggaa acggcaggta
    19321 atgttcacta tcttgcactt tcagacaagt cctttgtaat aatctgttac atataactga
    19381 ggcggggaat tcaaccattc cgggaaatgt tatccagcgt taaatttttc aaaaaacgta
    19441 acggcagatt ttttcactgc attgagaggg ggatgatggc tttacggaga tatgttctgc
    19501 tatttagcgt ttttcctagt gagatagtcc agtttctgaa aaatagccag tgtaatgttt
    19561 tgcaggtcaa tatttgttgc gtgtgtaaat ttaaatcatt ttaacctgat tcttgcaatg
    19621 tttttacatt gccacagcat cgtgatcttg atcacgaatg aatagaaaat ttcgctatta
    19681 aaaaaacaat taacctgcat ttttggttat ccgcaaacgg attacctgat gataacgcta
    19741 agcaatggca tcattgacgt taaaaagatg ttttaaaaga ggataaagaa aggttatcaa
    19801 tggggtaatc ggtgttaccc cttttcttca gatgtcaact ttctctttgt attcacaaag
    19861 atcttcaata atacaagagc cacagcgggg cttgcgggca atgcaggtat aacgcccgtg
    19921 caggatcaac caatggtggc agtcgacttt aaactctgcc ggaaccactt tcagtagctt
    19981 ttcttctacc tgttcgacgt ttttccccgg cgcaaattga gtacgattac aaacgcggaa
    20041 aatgtgcgtg tcgacagcaa tagtcggcca gccgaatgca gtgtttaata cgacgttggc
    20101 tgttttacga cctacgccgg gcagggcttc aagcgcagca cgatcttccg gaacctcgcc
    20161 attatgttgt tccagtaata tacggcaggt tttgatgata ttttctgctt tgctgttata
    20221 aaggccaatc gttttgatat aggttttcac cccttcaacg cccagttcaa gcatcgctgc
    20281 aggcgtattc gccaccgggt agagtttcgc cgtcgcctta ttaacactga tatcggtcgc
    20341 ctgagcggaa agcagtacgg caatcagcaa ttcaaaaggc gaactgaaat taagctcggt
    20401 ggtgggatga ggattgttct cacgcaggcg agtgaggatc tccagacgtt ttgctttgtt
    20461 catcagacat tccctgtttc accgtttggc aatgcacgtt cggcagctgc ttcagcacgg
    20521 cgctttttca ttctttcatc aatcaggtat tttcctgcca gcatcagtcc caagccaata
    20581 aatgcacctg gtggcagcat cgccagcagg aaaggggagt cggtgtggaa aatctccacg
    20641 cgtaatactt ttgcccagct acctaacagc gcatctgcac cgtcaaacaa cgtgccattg
    20701 ccgataattt cgcgtagtga acccagcacg aacatggcgc aggttgcgcc cgtaccaatt
    20761 gaaaagccgt ccagtgccga aagcgccgga ccttttttgg cggcgaaggc ttcagcgcgg
    20821 cccacaacga tacagttagt gacaatcagc ggaataaaaa tccctaatga ttgatacagg
    20881 ccaaaggcgt aggcgttgat cagcatctgt acagcgctga ccaccgaggc gatgatcatc
    20941 acgtaaatgg gaatgcggat ctcggctggc gtccagtgac gcagcgtcga aatggtcagg
    21001 ttggtcagcg tcagtaccag cgtagtcgca agtcctaaac ccagagcgtt agtggcagtg
    21061 gacgtgaccg ccaatagcgg acaaaggccg agcagttgga ccagtgcaga gttgtttttc
    21121 cacaacccct gaacaataac gtctttaatt tcgctcacgc tttattctcc acaggcagga
    21181 agttgagaaa gttgtgccgg taacgtctga gcgtacaatc cggcgcgttt taccgcatta
    21241 accaccgcgc ggggagtaat cgtcgcgccg gtgaactggt cgaaatcacc accatctttc
    21301 ttcaccgccc agtgcgcatc atccgcacca ctgatttttt ttcccgcaaa atgggtgagc
    21361 cagtcagaaa ggcgcaattc gattttatcg ccaagccctg gcgtttcgtg gtgctctgtc
    21421 acgcgcgtgc caagtaccgt gccattaaaa tcggctccca ccagcagctg aatcgcaccg
    21481 gaatagccat ctggcgcggt tgcttccaga acggcggcta ccggtttgtc atcctgtttg
    21541 gcgatgtaaa cccgatgctc acctttacct aactctggcg cagttaccag atagcaactc
    21601 tgtgcgagcg cattgttata gcgttcggct ggcagcacct gaccaaataa cgccttttgt
    21661 tgcagactgg cctgttcagc aattgtcgtt ttggtcatct ggttgatggc cgcagttaac
    21721 cctgtagaac ccgctgcaaa tagtgccagt gtaatgccgt gttttcggat agttttcagc
    21781 atggtttacc ctttgcgatg gccgtagacg cgcggacgcg tgtagtaatc gatcagagga
    21841 accgtgatgt tcgccagcag gacggcaaaa gccacgccgt caggatagcc gccgaaactg
    21901 cggatcaacc agactaataa gcccgccagc gcgccgaaca tcagccgacc acgattggtc
    21961 gtagaagcag taactgggtc agtcaaaata aagaatgcgc cgagcatagt cgcaccagac
    22021 agcagatgaa tttgcggtgc tgccagtgtt tctggtgaga acaaccagcc caacgttgcg
    22081 cataacgcca gcgttactaa gaagctgagg ggaatatgcc agcgaatcgc tttctgccat
    22141 agcaaccaca cgccaccagc cagccaggcg agatttaccc attgccaacc agcgcccgcc
    22201 agaataccgc tgtagatcgg atattgcata atctgttcaa ccgaatgacc tgcgcggaca
    22261 gaggttttga atgtatccag cggtgtcgcc tgactaatgc catcaatacc taagcgtagc
    22321 gtattcatat caccaccact ggcggtatga ccgctgaaaa taacctgtat ggcgtcgata
    22381 aaaccaggga tgttgaccgc aatttcatgt ggcggtaacc agctggtcat ctgcaccggg
    22441 aaggagatca gtaagaccac ataaccaatc attgccggat taaacgggtt ttgccccaga
    22501 ccgccataca actgtttagc gatgatcacc gcaaacaccg tacccagtac gaccatccac
    22561 catggcgcta gggggggaat acttaccgcc agcaataagc ctgtcagcaa tgctgagtta
    22621 tctttcaacg ttgcggctac cgactgcttg cgtagtttga gtacgagagc ttcggctaac
    22681 agagcactga ccgacgccaa caggatctga acgagagtac cccaaccaaa aaaccacagt
    22741 tgcgctgcga ttcctggcac ggctgcgagc aacaccaata gcataatgcg cgatgtctgg
    22801 cgctggttat gggtataagg ggagctagct attctgaata ccatttagtc ctcgtttaca
    22861 accttttgct gggcggcttt tttggcctga acgcgggcaa tagccgcggc aactgcggct
    22921 ttgcgcggat cgacctgttc ttctggttcc gcattagcct gttgctgttc cagcttgcgt
    22981 gctttggcgc gggcaatagc ggcttcgacg gcagctttgc gcggatcaac ctgttcttct
    23041 ggctccgcat tggcctgttg ctgttccagc ttgcgtgctt tggcgcgggc aatagcggct
    23101 tcgacggcgg ctttgcgcgg atcgacctgt tcttctggtt ccgcattagc ctgttgctgt
    23161 tccagcttgc gcgctttgga gcgggcgata gctgcttcaa cggcagtttt acgtggttca
    23221 gcaacggttg ctgcgtcgtt agtttgctcc agttctgcct gtttcgctct ggcttgcgct
    23281 ttacgggctt cccgtgctgc aataattgca ctgttatccg ggcgttcgcc cgctttaatc
    23341 acaataggct gtgtagcctg ggcctgtttc tctttcaccc gcgccagagc ggcagcaatc
    23401 gcatctttat ctttggctgc aggttgaacg gctgcgctct tatgtcgttc aaggcgagcc
    23461 gctttttcgc gctccagacg agcctggcgc gcttcgaaac gcgctttggc ttctgcggcg
    23521 cgcttttctt cctgacgaat agccgcaatt tcagcttttt cctgacggaa atattgcacc
    23581 aagggaatat tgctcgggca aacccacgcg caagccccac attcaatgca gtcggcaatg
    23641 ttatgcgtgg tagctttatc gtgttgctga cctttgctga accagtacaa ctgttgcggc
    23701 aaaagatcca cagggcaggc gtcagcacag gcgctacacc ggatgcagct ttgttcttcc
    23761 tgtggttcgc caagttcatt ggcagaggga gccaacagac agttggtaat ctttacgacc
    23821 gggacatcca gccatggcaa ggtaaagccc attagcgggc cacccataat caccatttga
    23881 tcggcagagg ggcagaatcc ggcatcattc aataaatgac gcactggcgt tcccagccgt
    23941 gcccagacgt tgcccgggcg agcgattgct tcgccagtca gggttacaac acgctcggta
    24001 atcggctcgc catcaataac ggcacgtttc actgcataag cagtgccgac gttttgcatt
    24061 aatacgccga tatcggatga acgcccgcca tgtggaacct gcttcccggt cagaatgtag
    24121 gttaattgtt tagcaccgcc agaaggatat ttggttggaa tcacccgcag agaaatatcg
    24181 ttagagtccg ccagcaccgc gcgcagcatg gaaatcgcct gcggtttgtt atcttcaatg
    24241 ccgataagaa tttcgcgtgg ctgcagaata tgcgcaagaa tgcgaatacc ctctacgacc
    24301 tgagccgcgc aatcctgcat caaacggtca tcggcggtaa tgtacggctc gcactcagca
    24361 gcgttgataa tcaacgtttc aatcttatct ccaccaccct gcaatttaac gcctgtcggg
    24421 aatccggcac cgcccagccc ggcaacacca aactgatgta tgcgctcgat taactcttcg
    24481 cgactgcgag agcgataatc gacccagccg tcgcgcggga tccagcagtc ttcaccatcg
    24541 gcatcaataa tcacgcttaa ttcagctaaa gctgaaggat gagccgtaga gtggggcgca
    24601 atagccgtaa cggtacccga ggtgggcgcg tgaacaggca gcattttgcc gcgaccacgg
    24661 gtaagcggct ggccgcgcaa tactttatcg ccgacgctaa cgcacaactc accttcagcg
    24721 ccaatatgct gtttcagtgg aataacaaaa cgctgcgcca gtggtacctg gcgcaggggg
    24781 gtaccgttgg actgggtttt catctccggt ggatggatgc cgccgttgaa atcccagatt
    24841 ttattttttc tgaatgcaga gaataactta agcatggtgt tccacgggaa tgatacgcac
    24901 gggaatggtg ttcagatccc atttccagga gtcaggtgtt tctgcgaccg gttgcaacga
    24961 gatgcagtgc gtcgggcacg gatcaacaca taaattgcag cccgtacaga gatcactcat
    25021 taccgtatgc atggcacggg tagcgccaac gatggcgtct accggacaag cctgaataca
    25081 tttagtgcag ccaatacagt tattttcatc aataaccgcc accatccgcg caggcgttag
    25141 ctcttgcgct tcgccatcca gcggctgcgg ctcgacatta agcaactcgg caatttttag
    25201 catcacagct tcgccacctg gggcgcaacg gttgattttt tcaccgttac agctgatggc
    25261 ttccgcgtag gggcgacagc cgggataacc gcactgacca cactggctct gcggtaagat
    25321 ttcgtcaatt ttctcaacga ccggatcgtc ttccaccgca aaacggcggg aggcataacc
    25381 cagaatggcg ccaaacgcca ggcccagcag gctcacggcg gcaacggcaa tccagatagc
    25441 attcattaca acttcaccaa accactaaag cccataaagg ccagagacat aagacctgcg
    25501 gtaattaacg caatggcatt accgcgaaaa ggtgccggga catcagccac agcaaggcgt
    25561 tcgcggatgg tggcgaagag caccatcacc agcgagaaac cgacagcggc ggaaaaaccg
    25621 tacagcgccg actgcaagaa attgtgcccg agattgatat tcagcaacgc cacgccgagc
    25681 actgcacagt tggtggtgat aagcggcaaa aaaatcccca gcagacggta aagcaccggg
    25741 ctggttttgc gcaccaccat ctcggtgaac tgcacgacca cagcaatcac cagaataaat
    25801 gccagggtgc gtaggtaaat cagattaagt gggatcaaaa tccacgtatc gataagccag
    25861 gcgcaaatag acgtcagcgt catcacaaac gttgttgcca gccccatgcc catcgcggtt
    25921 tccagttttt tggaaacccc cataaacgga cagagaccga gaaacttgac cagtacaaag
    25981 ttattgacca gtacagttcc gacaaacagt aacaggtagt cagtcatttt tcggcctgaa
    26041 ataaaaaagc cgcctattat ccgttaatcc agagcaggcg acaacaggtt aactgtaagg
    26101 ttattacgga ttcacaaacg tcgcttttac ccgcgacgag cgtttgaaat aggggacgat
    26161 caatgcggtt gccaacaaag taaacagcaa ctggcgaacg gctatgccaa cttccacggg
    26221 tgaaaaagcg aatgctttta ctgccagcaa tacggaaata agcagccaga taatatagtg
    26281 tttaggaaca caacgccgac gtttaaagaa agcgatggtc aaccacagtg tgtaatacca
    26341 catggcgata gcggtaataa aagaaacgcc ccataagatt tgtgtcgtca gtgcttgtcc
    26401 gccgagcgtt tgaaatgttt gcggagaaga taacgcagcg gtatacagca acaacgccag
    26461 cgtcgtactc aacagagcga ctaaaagcca ggccaacggg ccaagcaacc agcctccaat
    26521 acgttgtggc gttgtggtgg tcataggttc tccagaatag cgacgcaaac aggcaaatct
    26581 aattttaggg gcgttattat aaggctttta taaccgatcg ctactttctt tattggacat
    26641 agtgccagac ggacttcggt acctggccga ggtcaaacaa acgcccgcca gaaaccagct
    26701 ctgcgcgacg gtgatcggca gcacgataca tattgatcag ttcctgatcg tccgtcaggg
    26761 tgtaattaag atggtcgtag agtttttcca gactttcgag tgaactgatt ttgcgaaatt
    26821 ttaataagta gtcctgaaca gtcataaaag gtccataaaa taattaatac caaaggggta
    26881 ggtattatta gtgatgataa aatttcacca aaagtgaacg cttgtgttat tacaataata
    26941 agaatgcgct gatggcaaat attcagggat gaaaaactgc tctgaaaaga gtaacgcgct
    27001 ttactatccc tgacaatcac caacaacatc gaacaagata ataaattcct ggtttaatat
    27061 ccgacaagtg aaaacatgca cccggacggg cagcatgtcg ctccacaagt gcagagctta
    27121 cttgtgttgt accgaagcac tctgttcagg tggctgatag ttgtcaatgt gactcgccac
    27181 gccaagaaga atgactgaaa cgacaagaac gatccaacct gttaattcaa taagacgatt
    27241 cattacagcc cacatctctc ttgattgatc cattaacttc agggggtaaa tgttacttag
    27301 caataatagc tccagcaaga tttttactga ggtttttgcg atatttagct tttgtcgttg
    27361 gaaaattcgc tatttttttg acttaagtta aacaacatcc cttattgctg gcaggttatt
    27421 aaactgttga gcgtgggtaa aggatagtgt caaatagcca tcatacttca atgagaggca
    27481 atgacatgag cgacaacatc cgtgttgggt tgattgggta tggttatgcg agcaaaacct
    27541 tccatgcgcc cctgattgcg ggcacgcccg ggctggaact ggcggtaatc tccagcagtg
    27601 atgaaacaaa agtaaaagcc gactggccaa cggttacggt tgtctctgag ccgaagcatc
    27661 tgtttaacga tcccaacata gacctgattg tcattcctac acccaacgat acccatttcc
    27721 cgttagccaa agcggcgctt gaggcgggta aacatgtggt cgttgataaa ccctttaccg
    27781 tgacactgtc acaagcgcga gagctggatg cgctggcaaa aagcctgggg cgtgtgctgt
    27841 ctgtattcca taaccgtcgt tgggatagcg atttcttgac gctaaaaggt ttgctcgcgg
    27901 aaggcgtgct gggtgaagtt gcttactttg agtctcattt tgaccgcttc cgtccgcagg
    27961 tgcgcgatcg ttggcgtgaa cagggcggcc caggcagcgg tatctggtac gatttagcac
    28021 cgcatcttct tgatcaggcc attacgctat ttggtttacc ggtcagcatg acggtagatt
    28081 tggcacagtt acggcccgga gcgcagtcga ccgattattt ccacgccatc ttgtcctatc
    28141 cacagcggcg agtcatttta cacggtacca tgctggcagc cgctgagtca gcacggtata
    28201 tcgtgcatgg atcccgaggc agttatgtga aatatggcct cgatccacag gaagaacgtc
    28261 tgaaaaatgg cgagcgtctg ccgcaggaag actggggcta cgatatgcgt gatggcgtac
    28321 ttacccgcgt ggaaggtgag gaacgtgtcg aagaaacgct gttgacggtg cctgggaatt
    28381 atccggctta ctatgcggcg attcgtgatg cgttaaatgg cgatggtgaa aatccggttc
    28441 cggcaagcca ggcaatccag gtaatggagt tgattgagct gggcatcgaa tccgccaaac
    28501 atcgcgcgac gttgtgcctt gcgtgaaaaa tggatggtgc aatttgcacc atccattttt
    28561 gttacttcgc ggcgactttt tctcgcagtg cgcgtttttc ctctgcgctg aggaaagcca
    28621 tttccagacc attaatctgt gcctggcgga tttgctcacg ggataaccca gcagctggcg
    28681 cggcaacggt atattcgtga atgatatcca ctccctgtac gccgggatcg tcagtgttaa
    28741 tgctggcacg aatgccatgc tcaaggaacg ttttcagcgg atgtgcagcc agctctgcta
    28801 cggtgctggt ctgaatattg gaggtcagac aggattcaat accaatttgt tgctcggcga
    28861 gaaaatccat cagcgcccga tcttcaatgg cttttacgcc atgtccaata cgctccgcac
    28921 ccagttcacg aatcgcctgc cagatgcttt ccggcccggc agcttcgcct gcatggacgg
    28981 taatatgcca gcccgcatca cgcgcgcggt tgaagtgaga aaggaacaga cttcccggga
    29041 atccaagttc atcaccagct aaatcaagtg cggtaatctg gtcacggtgg gctaaaaagg
    29101 cctccagctc ttgctgacag gcggcttcgc cgaaggttcg gctcataatg ccgataagct
    29161 tcgcctgcac accaaaggtg cggcaacctt cacgtacgcc atcgatcacc gcttcgacaa
    29221 cacccgctac aggcagctta tgtgccattg ccatgtagcc tggtgaaaaa cgcagctcga
    29281 catagcgcag gccgttacgg gctgcatctt caatgttttc aaatgccacg cggcggcagg
    29341 catcaagaga ggcgagaact ttaacgcccc agtcaagttt ggtcagaaag ctcaccagat
    29401 cgggttcgtt ggcaatgacc tgaacgtggg gaatcagtgt ttcaagggat tgtgcaggaa
    29461 gcgagatatt atactggcgg ccaagttcaa gaatggtctg gggacgaatg ttgccatcaa
    29521 ggtggcgatg gatatcagtt aatggcaggg tggtatcaat catggtcgca ctctttttct
    29581 ggatataaaa gtgcgcacca ttataaaaac aaagcagggt aaaaatccac gattgcgcaa
    29641 cgatttttac gttgcgcaat tggggttagc gaacagcgcg gatggcgttt attaatccag
    29701 ccacaccttt ttccagtttc gaacgtgggc agccggcatt gagacggaca aaaccacgac
    29761 cttcttcacc ataggtatac cccggcatga tcgcgacttt ttcttgctcg ataagcgctt
    29821 tttgcaacgc gttgtcgtca atattcaacg gacgtagatc aagccaggcc agataagtgg
    29881 attgcgggat ctgccagttg agttcaggaa acgcggcgtt cattttatct gagatatacg
    29941 tcaggttatc tttcagatag acgcgtaagg catccagcca cggtgcacct tgctgatagg
    30001 cggcgatatg ggcagttaac gccagtaccg aaggggaaga aagcccatca cgtcctttca
    30061 gtgccgataa ataggcatca cggctgctgc tattttctat aatcccgtaa gcaccggtca
    30121 gcgcgggaat attgaaactt ttcgatcccg acgtcagcaa cgcccagtct ccccgcgcca
    30181 cattactcca gggaatatgc ggctgctcgc cccagaccat atccatatgg atttcatcgg
    30241 aaataacccg cacatcatga cgctcgcaca ggtcagccat gatctccagc tcatcgcacg
    30301 tccacacttt cccggtagga ttctgtgggt tacacaggag cataatttta cattctggtt
    30361 tcgccaaaac ggcttccagc ttgcccatat cgcaaaacca gccgtcagcc tgcttctcta
    30421 aagcaacggg cattactgtg cgctggttac cttcaatggc cttgtaaaat gcgtcatagg
    30481 cgggcgtgtg gatcaccacg ccttcacctg tttcagacca ctgacgaatc agttctgaaa
    30541 ccatatagat gacagaaggg ccatacacca ccgtctgcgt atcaattgcg gtgtaatgct
    30601 gggtggaaaa ccagtgggca atagccgcga gaaactcatc gtttttccag cggctgtagc
    30661 caaatacgcc gtgcatcagg cgctgattca gcgcctcgat aatgcagggg gcagtggcaa
    30721 aatccatgtc tgaaatcgtg aacggtaaca ggtcagcagt gccgaaacgg tcagcgacat
    30781 aatcccactg tgtacaccat gtgccatgac gatccacgac ctttgaaaaa tcgaacatat
    30841 cttatcctta tgcctggaca gtatgcatca gaccggccat ttcatctttt actgactgaa
    30901 cttgtggccc gataacaacc tgcaggttat gttgattaag ttgtactacg ccaattgccc
    30961 gattgtcctt cagtgcttgc acattaacaa gcgacatatc ttccacagac aaacgcagac
    31021 gggtaatgca gttatcgagg ctgacaatat tgtcggcacc gcctaatgct tcgaggattg
    31081 caggaacgtt gtaacctgat ttacccggcg caccggcaac ggctttttcg attgagctgg
    31141 caacttcgct atcgcgcccc ggggttttca gattgaagcg ggtgatagcg aaacggaaga
    31201 tgacgtagta aacgacaaac cagattgccg ccaccactgg caccatgtac cacttggttg
    31261 acagaccatg caaaataccg aacaccacga agtcgatgat attgccgtcg gtattaccga
    31321 tggtgacgcc gagcacagac atgacggtga agccgaggcc ggttaacagc gcgtggatga
    31381 catacagaac tggcgctacg aacaggaaca ggaattccag cggttcggta gtgccgccaa
    31441 cgacgcaggc gatcaggcca gaaatcagca gacctttaat tttatggcga ttttccgggc
    31501 gcgcgcagtg atacatagct aacgctgcac ctggcagacc gccgagaaac gcaggcattt
    31561 taccttgcga aaggaaacgc gtgacgcttt cagaaaaacc gtgagtggtc gggcaactca
    31621 attgcgcctg gaagatggtc agtgcgccgc tgacggtttg accgcagacc tcctgcgtgc
    31681 cgcctgcgtc ggtaaagcga attaatgcca ccagaatgtg atgcagacca aacggtaaca
    31741 gcagacgttc accggtacca aacagcatcg gtccgaaatc acccgcgctg tttatcatat
    31801 ggcccaagcc gctaataccc atggcgaaaa tcggccagac taatggaatc accaggccga
    31861 caaggcccat caccagcgag gagataattg gtacgaagcg cgtaccgccg aagaatgcca
    31921 gcgcatccgg caggcggata ttatggaaac gctcatgcag catccagacg ataataccgg
    31981 cgatcaccgc accgaggatc ccggtatcga tcgactggat cccaagaatg ctctggatgt
    32041 tattggcttt cagaacggcg gcatctgtgg ttggcagaat gcctttattg gtcaaccaga
    32101 agtttaccgc gaggttcatt accgcataac cgacgaagcc agcgaatgcc gctacgcctt
    32161 tattttcgcg tgccaggccc agcgggatgg cgatacagaa catgacaggc aggaaactaa
    32221 aagcaaacga gccaatctta ctcatccagg taaagatagc ctgcaacacg gggttgccca
    32281 ggaccgggat cagggttatg acatcatggc tgctaagaga actaccaatg ccgagcataa
    32341 tgccgcagaa cgacaataat gccacgggta acatgaaggt tttgcctaac tgctggaaga
    32401 actcccacag cgtgactttc ggtgctgttt tcgccgtcat aaaacgactc ctcgtagaga
    32461 acaatctgga ttcagtaaat tgcgcgagaa gataaaacgt tttatcaaat tttagtgagt
    32521 cataaatcac attacgcaac gataatagcg ggtataagat aaataaaagg taaaacgttt
    32581 tatctgtcac ataatcaggg agtaggtcat ctgcatggct accgccaaaa aaataaccat
    32641 tcatgatgtt gcgctggctg cgggcgtgtc ggtaagtacc gtttcgctgg tgcttagtgg
    32701 caaagggcga atctctaccg ccacaggaga acgcgttaac gccgccattg aagagctggg
    32761 atttgtgcgc aatcgccagg cgtcggcgct gcgcggcggg caaagcggcg tcattggttt
    32821 gatcgtccgt gatttatctg cgccgtttta cgccgaattg acggccggat tgacggaagc
    32881 tctggaagcg cagggacgga tggttttttt gcttcacggc ggtaaagacg gcgagcagct
    32941 ggcacagcgg ttttcactgt tactgaatca gggtgtcgat ggtgtggtaa ttgccggggc
    33001 tgcaggaagc agcgatgacc tgcggcggat ggcagaagaa aaagctatcc cggtgatttt
    33061 cgcttcccgt gccagttatc ttgatgatgt tgatacggtt cgcccggaca acatgcaggc
    33121 tgcacagttg ttgacggagc atctcattcg caatgggcat cagcggatcg cctggctggg
    33181 agggcaaagt tcctcattaa cccgtgcaga acgggtgggg ggctattgtg caactctact
    33241 aaaatttggc ctgccgtttc acagcgattg ggtgttggag tgcacttcca gccagaagca
    33301 agccgcggaa gctatcacgg cgcttttacg tcataacccg accatcagtg ccgtggtttg
    33361 ctataacgaa actattgcga tgggggcatg gtttggtttg ctgaaagcag ggcggcaaag
    33421 cggggaaagc ggagtcgatc gttactttga gcaacaggtt tctctggcgg catttaccga
    33481 tgcgacacca accacacttg atgatatacc tgttacctgg gccagcacgc cagcgcggga
    33541 acttggtatc acacttgcgg atcgcatgat gcaaaaaatc acccatgaag agacgcattc
    33601 acgcaatctt attattcccg cccggctcat tgcggcgaaa taatcctctc tttatctgct
    33661 atacttggta gtgtcctttc ctcaaggtta atgataaaaa atgtggcaca caggcggtgc
    33721 cttttatttt tactgaagtt cacaggaggt ttatgtgttt aattctgaca acctgagact
    33781 cgacggaaaa tgcgccatca tcacaggtgc gggtgcaggt attggtaaag aaatcgccat
    33841 tacattcgcg acagctggcg catctgtggt ggtcagtgat attaacgccg acgcagctaa
    33901 ccatgttgta gacgaaattc aacaactggg tggtcaggca ttttcctggc gttgtgatat
    33961 tacttccgaa caggaactct ctgcactggc agactttgcc gtcagtaaac tgggtaaagt
    34021 tgatatcctg gttaacaacg tcggtggcgg tggtcctaaa ccgtttgata tgccaatggc
    34081 agattttcgc cgtgcttatg aactgaatgt gttttctttt ttccatctgt cacaacttgt
    34141 tgcgccagaa atggaaaaaa atggcggtgg cgttattctg accatcactt ctatggcggc
    34201 agaaaataaa aatataaaca tgacttccta tgcatcatct aaagctgcgg ccagtcatct
    34261 ggtcagaaat atggcatttg acctgggtga aaaaaatatt cgggtgaatg gcattgcgcc
    34321 gggggcaata ttaaccgatg ccctgaaatc cgttattaca cca
//
LOCUS       DAJVQH010000027        34266 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJVQH010000027 DAJVQH010000000
VERSION     DAJVQH010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.027"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(95..2191)
                     /locus_tag="OQM87_001425"
     CDS             complement(95..2191)
                     /locus_tag="OQM87_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1507612.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRLAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
     gene            complement(2193..2444)
                     /locus_tag="OQM87_001426"
     CDS             complement(2193..2444)
                     /locus_tag="OQM87_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1507613.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            2597..3160
                     /locus_tag="OQM87_001427"
     CDS             2597..3160
                     /locus_tag="OQM87_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507614.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(3436..3858)
                     /locus_tag="OQM87_001428"
                     /pseudo
     CDS             complement(3436..3858)
                     /locus_tag="OQM87_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(3858..4451)
                     /locus_tag="OQM87_001429"
     CDS             complement(3858..4451)
                     /locus_tag="OQM87_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCS1507615.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            4598..5005
                     /locus_tag="OQM87_001430"
     CDS             4598..5005
                     /locus_tag="OQM87_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCS1507616.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(5125..6117)
                     /gene="rpoS"
                     /locus_tag="OQM87_001431"
     CDS             complement(5125..6117)
                     /gene="rpoS"
                     /locus_tag="OQM87_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCS1507617.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(6180..7319)
                     /gene="nlpD"
                     /locus_tag="OQM87_001432"
     CDS             complement(6180..7319)
                     /gene="nlpD"
                     /locus_tag="OQM87_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCS1507618.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(7459..8085)
                     /gene="pcm"
                     /locus_tag="OQM87_001433"
     CDS             complement(7459..8085)
                     /gene="pcm"
                     /locus_tag="OQM87_001433"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCS1507619.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(8079..8840)
                     /gene="surE"
                     /locus_tag="OQM87_001434"
     CDS             complement(8079..8840)
                     /gene="surE"
                     /locus_tag="OQM87_001434"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCS1507620.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(8821..9870)
                     /gene="truD"
                     /locus_tag="OQM87_001435"
     CDS             complement(8821..9870)
                     /gene="truD"
                     /locus_tag="OQM87_001435"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCS1507621.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICAKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(9867..10346)
                     /gene="ispF"
                     /locus_tag="OQM87_001436"
     CDS             complement(9867..10346)
                     /gene="ispF"
                     /locus_tag="OQM87_001436"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCS1507622.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(10346..11056)
                     /gene="ispD"
                     /locus_tag="OQM87_001437"
     CDS             complement(10346..11056)
                     /gene="ispD"
                     /locus_tag="OQM87_001437"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCS1507623.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(11075..11386)
                     /gene="ftsB"
                     /locus_tag="OQM87_001438"
     CDS             complement(11075..11386)
                     /gene="ftsB"
                     /locus_tag="OQM87_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCS1507624.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(11580..11903)
                     /locus_tag="OQM87_001439"
     CDS             complement(11580..11903)
                     /locus_tag="OQM87_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCS1507625.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(11953..12558)
                     /gene="cysC"
                     /locus_tag="OQM87_001440"
     CDS             complement(11953..12558)
                     /gene="cysC"
                     /locus_tag="OQM87_001440"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCS1507626.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(12558..13985)
                     /gene="cysN"
                     /locus_tag="OQM87_001441"
     CDS             complement(12558..13985)
                     /gene="cysN"
                     /locus_tag="OQM87_001441"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCS1507627.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(13987..14895)
                     /gene="cysD"
                     /locus_tag="OQM87_001442"
     CDS             complement(13987..14895)
                     /gene="cysD"
                     /locus_tag="OQM87_001442"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCS1507628.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15147..16184
                     /gene="iap"
                     /locus_tag="OQM87_001443"
     CDS             15147..16184
                     /gene="iap"
                     /locus_tag="OQM87_001443"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCS1507629.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(16330..17421)
                     /locus_tag="OQM87_001444"
                     /pseudo
     CDS             complement(16330..17421)
                     /locus_tag="OQM87_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(17615..17767)
                     /locus_tag="OQM87_001445"
     CDS             complement(17615..17767)
                     /locus_tag="OQM87_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCS1507630.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(18031..18765)
                     /gene="cysH"
                     /locus_tag="OQM87_001446"
     CDS             complement(18031..18765)
                     /gene="cysH"
                     /locus_tag="OQM87_001446"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCS1507631.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMVEEETRFFGLKRECGLHEG"
     gene            complement(18839..20551)
                     /gene="cysI"
                     /locus_tag="OQM87_001447"
     CDS             complement(18839..20551)
                     /gene="cysI"
                     /locus_tag="OQM87_001447"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCS1507632.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(20551..22350)
                     /gene="cysJ"
                     /locus_tag="OQM87_001448"
     CDS             complement(20551..22350)
                     /gene="cysJ"
                     /locus_tag="OQM87_001448"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCS1507633.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            22669..23031
                     /gene="queD"
                     /locus_tag="OQM87_001449"
     CDS             22669..23031
                     /gene="queD"
                     /locus_tag="OQM87_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCS1507634.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            23109..24380
                     /locus_tag="OQM87_001450"
     CDS             23109..24380
                     /locus_tag="OQM87_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1507635.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            24371..24631
                     /locus_tag="OQM87_001451"
     CDS             24371..24631
                     /locus_tag="OQM87_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1507636.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            24648..25223
                     /locus_tag="OQM87_001452"
     CDS             24648..25223
                     /locus_tag="OQM87_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCS1507637.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(25371..26231)
                     /locus_tag="OQM87_001453"
     CDS             complement(25371..26231)
                     /locus_tag="OQM87_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS1507638.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIDVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(26228..27007)
                     /locus_tag="OQM87_001454"
     CDS             complement(26228..27007)
                     /locus_tag="OQM87_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCS1507639.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTLVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(26985..28394)
                     /locus_tag="OQM87_001455"
     CDS             complement(26985..28394)
                     /locus_tag="OQM87_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1507640.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMSGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SIFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(28416..29870)
                     /locus_tag="OQM87_001456"
                     /pseudo
     CDS             complement(28416..29870)
                     /locus_tag="OQM87_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(29940..30726)
                     /locus_tag="OQM87_001457"
                     /pseudo
     CDS             complement(29940..30726)
                     /locus_tag="OQM87_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            31045..32322
                     /locus_tag="OQM87_001458"
     CDS             31045..32322
                     /locus_tag="OQM87_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1507641.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            32349..33819
                     /locus_tag="OQM87_001459"
                     /pseudo
     CDS             32349..33819
                     /locus_tag="OQM87_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT         8273 a         9067 c         8427 g         8499 t
ORIGIN      
        1 catcatcaat atcagtcata gtatttcctc ggttatttca aatcagcttt gtttccttta
       61 tttaacagtc cgttcccgcg gactgttttt ttattcaggc cagcggatca tcttcttcct
      121 gcatggcaaa aagtttttgc atccccagcc ggaaatcgcg ttcaaattct tcgtcatgct
      181 cttcttcata ttccggttgc tgaactgcca ccacattacc aaccgggcga taaacattac
      241 tcagccaggg ttcagcctgt ttgtgttcca gaatctgaat accttcatct tcagcatcac
      301 gaattttttc ctcagcgcgt ttgcgcattc ctttaatacg ctgctgttgt ttgtgatatt
      361 cagcactgac agcaaaggct gcgcgtttat tgccgttcca tatcgcctca cagataaagc
      421 tgccgtcctt gcgacgtacc gtaattcgct cggcgtcatg aatgtcataa ctgataagca
      481 ctttgcgacc gtgttcgtca cgcaactccg gcgcgtagta aatattgttc agccactgaa
      541 tttcgcagcg tcttaccggt cgttccacca ttgggcggaa catgtcgcgc agctcaagat
      601 cactcagcca ctcaatttcg gtgtcttctt ctgccaggcg tttttttctg aactctgtcg
      661 ggctgtaatg ccgtccgttc ggtttcatcg gcagctcaga atgcgggcgg ttgttgtacc
      721 attccacgcc ttcacgaata gcctcaataa gctccgccca cgatggcagc tcgcgcattg
      781 ccgctttctg gcggtcgttc agaggcttgt cctgttgcag ggcgttaaac gctgaacgca
      841 gatcacggtt aagtacacgc aacgattccc ggtcagcacc cttgccaaaa taggtgcgat
      901 acctcctggc tatacgcatc ggcagtgtcc ggttcaggcg ttcaataatg ccgcgtccct
      961 gtggattacc ggcaataccg gtagggtgtt taatccccag gcgtggcaga atacccacga
     1021 cctctttgtc cagcatgtca gccgtttcac cggaaccgtt atccgagtaa tacaggaacg
     1081 gttttccgtg gttgcgaatg ccgtgctgta tcgcaccggc aacggcgaac acgttttcgg
     1141 caaggtcaag actccagcca acaacaaacc gtgttccacc atcgatcaca aacgtgactt
     1201 ccggtgagaa tggacgcccg tggatcggat gcgcacattt cagcttcata ccgtgaccgt
     1261 caccaatcca gacataattc accggcatag ctgtccagtc gcggcgggtg aatccttcaa
     1321 gctgacggta ttcactgccg gtaacgcgcc ctttttgttt caccacttcc ggcagttttt
     1381 tcatggcata ccggacaaca tcataggaag gcatcacctc cagcatgtac ggttcgtccg
     1441 catggcgacg ctgccattct gcgacaaaat cctcgtaggc ttctgacatg ggtctgccgt
     1501 tcacctggcg atactgcgcc agaaattcag gcagccagct gatttcttcc ggttttattt
     1561 cgtcgcgttt tcccggtgcc agtaacagca gccgttcggc ggcgttctgc gactggttgt
     1621 atttcagaac ccactggtaa agtggatcgc ggctgatagt acgattagcg ccacgttttg
     1681 cattggcgtt aactgcggcc tgttgcaggc gctccggcag ctcaccttta cgggcttttt
     1741 caacaagaaa gttaatggct ttcagtcgcc cgaggttacc ggcgctttcc agcttcagca
     1801 cttccaccac cagcgcagct cttgcttcag atacccagcg ctggttttcg gtcagcgcat
     1861 tcaggcgttc ttccatcagt tgtggagaac cgcgatatgc ttcgattgcc tggacagcgt
     1921 caggattgcg tgtttttctg gtaacagctt tcacttcaga aacattgttc tctgtagcca
     1981 ccagctgttc cacatagcgt tcacgcagtg cctttcgtgt tatttcaggc agacaatcaa
     2041 tgtggtactc aatggctttt gtgccttccc gacggcgact aacgtcacca agatcacgag
     2101 aaaaacgctg taacgcctgt cggatgcctt ttgtggtggc tggcattcct ggtacgccga
     2161 gcaggtcatt tacggtaacg tacgactgca tatcacgacg ccttcctgat aaatccatcg
     2221 ttacgatacc ggctgggcca gatgatttct ggcggtacac ctatagcgtc agaaattacg
     2281 cgttcgtatt ttttgtttgg tgtgcgaact accgctttca gtgaatcctg agcataacca
     2341 gcatggagtg aaagctcccg gaaggaagac cacccgttat ctttgagcgc cagctgaatc
     2401 ttcagcgaag gccaatcgcg gtcactatct tctctttgtc tcatctttcc tttaacctac
     2461 aaaataaccc gcgcggatat ccgtatggat aaagattaag ataccaaaga ttccctataa
     2521 agggttcttt ggtgctttta tggtttattt tttaatttca ttatttatca atgaattaga
     2581 tagaaaggaa ccaagaatga gaaaagcacc ccaaggttct tttcctgccg atggaaaaga
     2641 accgattatc gaaagaatct ttaggttagt agaacgatac ccttcaagaa atgcggctgc
     2701 tcgggcatgg gggatcaatg aaggcacttt aaaaaattat tacaacaggc gggattacgc
     2761 accgatacca cgcccgcatc agttaaaaaa gatagcagaa agcgaaaatg ttaccttgga
     2821 atggctcatg acgggagagg gtgatgttga actaaaagat tccaaagaac ccaaaaaaga
     2881 gaatgcggat aatgttgatg ctcaaattct aatgttcttg tcatttctta aaccacaaga
     2941 aaaacaacaa gtagctgatg ttcttgggag aaaaggagcg gagcaattac tcattcttct
     3001 ggatgagcac atccaagagt tgcacgctct agttggagtt cgtagagcca tagcgttaag
     3061 ccttggtaat ttgccagacg aaaaggttag agagatttac gcgttgtccg aagctaaggg
     3121 cgatcactta aatctacctc agaaagcagc cagcgcataa cccagtgcaa gtgcctagct
     3181 gtgctaaaat tacattggtt ttggcgcttg cacccatcag cgctataata aacacctgaa
     3241 tcgtttttga acggtcttta aaggctattg taagaacctg tgagttatct taaatgtttt
     3301 attgatatcg aaccgtttaa catttttgga ctcgctttat cttgaaaacc ttgagttcct
     3361 aacttgttga ttccaatcta ttcccgtctt ttttaatctc ttcccggtta tcttgaataa
     3421 attacctagt catacgagat agaaaccgcc atcgccatca ttcaggcgaa acagcggcag
     3481 aatatcgaac aggttgatct cttcgccatc gacggtgttc tgcgcccagg ctggattggc
     3541 gcggcgctcc ggggcaatcg ggaagttatc ccagcggcgg ataaactcat caatctgctt
     3601 tttgaccggg gtgtttggtg gcaggccaag ggaaatcgcg tggttctgcc aggaaccgat
     3661 ggtattcatc gccacgcggg catcggtaaa gccgcgaata ttatcaaacc acagcgcggg
     3721 cgcgccgtcg ccgatacgcc cggtggcgtt agctgctgct gccagatccg gctcagcgtt
     3781 cacttcttcg ctgattttca gtaactggcc gtggtcatca agcgcctgta aaaagctgcg
     3841 taaatcatca aatgccatta ttcattctcc tgagaaaaat tccgggcctg cggcaatcct
     3901 tgccagcgcc tggcgtgggg atgttcgagg ccaaattgat ccagcacgcg ggctaccaca
     3961 tggtggacaa tgtcatctac cgtttcggga tggttataaa aggcaggcat cggcggcacc
     4021 atcgccacgc ccatgcgtga aagtgcgagc atattttcga gatggatggt gctaagcggc
     4081 atttcacgcg gcaccagcac cagtttgcgg ccttctttga gcacgacgtc cgccgcgcgc
     4141 cctaccaggc catcggcgta accagcgcgg ataccggcga gcgttttcat actgcacgga
     4201 ataacgatca tgccgtcagt acgaaaagaa ccggatgaga tgatcgccgc ctgatccgcc
     4261 gggttatggc tgaagtctgc gagggcagca acatcgcgag cgctgtaagg cgtttctagt
     4321 tcaatgatgg ttttcgccca cttcgacatc accagatgag tctcgacatt cggcatctca
     4381 cgcagcgctt gcagtaatgc cacaccaaga ggcgcaccgg tagcccctgt catcccgacg
     4441 atcagtttca ttgcttattc tcttaattgt tcgtatacga acattattat taacatactc
     4501 cttcatttcc acagcggcaa ggattgaaaa gtaacaacat cttcttcgtc actaaaaaac
     4561 cgctatgata gcgacagaga attagcccgg agtttacatg gcgttacgaa ataaagcgtt
     4621 tcatcagtta cgacagcttt ttcagcagca cacggctcgc tggcagcacg agttacctga
     4681 cctgaccaaa ccacagtacg cggtgatgcg cgccattgct gataaacctg gtattgaaca
     4741 ggtggcactg atagaagcag cagtcagcac caaagcaacg ctagcagaaa tgttggcaag
     4801 aatggaaaat cgtggcttag tcagacgtga acatgacgtc gctgacaagc ggcgacgctt
     4861 tgtctggctg actgccgaag gagaaaagct acttgctgcg gcgataccta ttggtgacag
     4921 cgtggatgag gaatttttgg ggcgtttgag tggagaagag caggagctgt ttatgcagtt
     4981 ggtgcgcaag atgatgagca catagaatgc tgattgccgg gtaggacgct gacgtgtctt
     5041 atccaggcga caaaaacaca agcttcccaa acaaaagaaa aaggccagcc tcgcttgaga
     5101 ctggcctttc tgacagatgc ttacttactc gcggaacagc gcttcgatat tcagcccctg
     5161 cgtttgcagg atttcgcgca aacggcgcag gccttcaacc tgaatctggc gaacacgttc
     5221 acgggtgagg ccaatttcac gacctacatc ttccagtgtt gccgcttcgt accccagcaa
     5281 accgaatcga cgtgccagta cttcacgctg tttggcgttc agctcgaaca gccatttgac
     5341 gatgctctgc ttcatatcgt catcttgcgt ggtatcttcc ggaccgttct ctttttcatc
     5401 ggccaggatg tccagcaacg ctttttcgga atcaccaccc agcggggtgt ctaccgaggt
     5461 aatgcgctcg ttaagacgaa gcatacggct gacgtcatca actggcttat ccagttgctc
     5521 tgcgatctct tccgcacttg gttcatggtc cagcttatgg gacaactcac gtgcggttcg
     5581 caggtaaacg ttcagctcct ttacgatgtg aatcggcaaa cgaatagtac gggtttggtt
     5641 cataatcgcc cgttcaatcg tctggcgaat ccaccaggtt gcgtatgttg agaagcggaa
     5701 accacgttcc gggtcaaact tctctaccgc gcggatcagc cccaagttgc cctcttcgat
     5761 aaggtccagc aacgccagac cacgattgcc ataacggcgg gcaattttta ccaccagacg
     5821 caagttactc tcgatcatcc ggcggcgaga ggcgacatct ccacgcagtg cgcgacgcgc
     5881 aaaataaact tcttcttcgg ccgttaacag tggtgaataa ccaatctcac caaggtaaag
     5941 ctgagtcgcg tccaacacac gctgtgtggc tccctgcgat aacagttcct cttcggccaa
     6001 atcgttatca ctgggttcct cttctactaa ggccttttcg tcaaaaacct caactccgtt
     6061 ctcatcaaat tccgcatctt catttaaatc atgaactttc agcgtattct gactcataag
     6121 gtggctccta cccgtgatcc cttgacggaa cattcaagca aaagcctggt tccgccgatt
     6181 tatcgctgcg gcaaataacg cagcgggttt acggatttcc ccttgtaacg aatttcaaaa
     6241 tgcaagcgtg ttgaactggt tccggtgcta cccatggttg ctattttttg ccccgccttc
     6301 acttcttgtt gttcccggac cagcattgtg tcgttatggg cgtaggcact caggtaatca
     6361 tcattatgtt tgatgataat cagattaccg tagccgcgca gcgcgttacc agcataaaca
     6421 acgcggccat ctgcggtcgc gataattgcc tgtcctttgc tgcctgcgat atcaatcccc
     6481 ttgttgcccc cctcagaagc gccaaaggtt tcgatcactt tgccctcagt cggccagcgc
     6541 caggtggaga taggcgtact ggttgatgta ctgctgacag tcggctcggt tgtgcttgct
     6601 gttggtaccg ttacaggcgc tgtgaccgtg gtcgcagttg gcttgttgtt cggcaacatt
     6661 ttgttagcac tctgttcacc cgaagactca gaatacgtaa ttgtcggttg cgacgcaaca
     6721 gcaacggtgg aattttgtgc aggcttgatc acaactcctt gctctgctgc gtcggcctgg
     6781 gtaatagcat ttccgccagt gattggcgta ccggaagcat tacccacctg caaggtctga
     6841 ccaacgttca gcgcgtatgg tgcctgaata ttgttgcgct gagcaaggtc acggaaatcg
     6901 ttgccagtaa tccaggcgat atagaaaagt gtgtcgcctt ttttcacggt ataggtactg
     6961 ccgctataac tgcctttcgg aatgttccca tactgacggt tatagacgat gcgtccgttt
     7021 tccatctgta ccggctgctg agctactggc tgcactggct ggatttgcgg ttgttgagta
     7081 gcctgaattt gtggctgctg taccggctga atttgcggtt gctgcgctgt agacgtcgtc
     7141 cctattttcg gcggcggcgt aatcaacata ccagaattag tatttgcagg cgcattgcca
     7201 ttaacggagc tgaccggtgc cggtggattt gaagtgtcag aacagcctgc cagccatagc
     7261 gaaaccagtg acaaagccgc aatgcggcga acggtgaatt ttgggcttcc cgcgctcatt
     7321 tatcccccag gaaaaattgg ttaataacca gtgacataat taccgtgcaa ggcaccctac
     7381 tgaacactgg aaaagatgtt cacgatacgc tgacctacgg caaaataacc aggaaaaatc
     7441 caggtatttc ctcacgtttt aagccagctc acccttcact aaagggacaa agcgcacggc
     7501 ctccacggta tcgataataa attcgcctcc ccgacgacgc acccgtttca aatactggtg
     7561 ctcctcccct acgggtaaga cgagaatccc gccttcgtcc agctgcgtca ttagcgcagt
     7621 tggaatttcc ggcggtgccg ccgtaacaat gatagcgtca aacggcgcac gtgcctgcca
     7681 accttgccat ccatcaccat gacgggttga aacattatgt aaatcaagat ttttcaggcg
     7741 gcgacgtgcc tgccactgca agcctttaat ccgttcaacc gagcaaacat gctggacaag
     7801 atgcgccagg attgccgttt gatatcccga accagtgcca atttccagca cccgcgactg
     7861 cggcgtcagc tcgagtaatt cggtcattcg cgccaccata tacggctgcg aaattgtctg
     7921 cccctgacct atcggcaagg cgatattgtc ccaggctttt tgttcaaacg cttcatcaac
     7981 gaatttttca cgcggcacgg cggcaagtgc attcagcacc tgctcatcct gaataccttg
     8041 cgcacgtaat tgatccagaa gtgcttgtac gcgtctgctt accattgcgt gccaactccc
     8101 acgctgttta accagtctga aaccacatct tgcgcgctat gcgcagttaa atccacatgc
     8161 agcggcgtga tggagacata gccctcatct accgcagcaa aatcggtccc cggaccggca
     8221 tcacatttac cgcccggcgg gccaatccag tacagcgtat tgccgcgcgg atcttgctgc
     8281 gggatcacct gatctgccgg atgtcgtgta ccgcagcgcg tcacgcgaat acctttgatt
     8341 tgatccaagg gtaaatccgg aacgttaata ttaagaatac gtccggtgcg cagcggctct
     8401 ttacacagtg cgcgcaaaat tgaacaggtt accgccgcgg cagtgtcgta atgtttatgc
     8461 ccgtcaagcg agacggccag cgccggaaaa cctaaatgac ggccttccat cgcggcggct
     8521 accgtaccgg aataaataac atcatccccc agattcggcc cggcgttaat tccggacaca
     8581 acaatgtccg ggcgcggacg catcagagca ttcacgccaa gatagacaca atcggtcggg
     8641 gttcccattt gcacagcaat atcaccattt tcaaaggtaa acgtgcgcag ggaggattcc
     8701 agtgtcagag aatttgaagc gctgctgcgg ttacgatcgg gggcgaccac ctgaacgtca
     8761 gcaaactcac gcaaggcttt cgccagcgtt tgtataccgg gtgcatgtac cccgtcatca
     8821 ttactcagca atatgcgcat aatcacctgt tgtgttgata agttccctga caacgctggt
     8881 tgcaaaacta cccgccggaa gccagaaacg gatctctacg gtgacgtcat cccaccagtt
     8941 ccagcttaat tgttgcggat acagcagcat cgctctgcgc gcggcttcaa ctttttcgcg
     9001 caccagtaaa gcttgtaatt cagtttctgc ggcgacagct gcttgttcga atgccagcgc
     9061 ttcacgctga gttccccatt cgccactgcc cggcaatgcg gcggttatca tcagctcttt
     9121 atcgttgacg cgacgctgta attccgccag ttcttcggtg gttgctacaa accagctacc
     9181 acgtccggct aattgtagcg catcgccgtc aacaacttga ttaacgtctg cttttttgag
     9241 gcgctcagca acaatctgat taaacaacgc actgcgggct gccgacaacc aaaaactccg
     9301 tttattgcga tcgcgcaccg gagtattggt ttgcgcccag cgcagcgcgc cctgcaagtt
     9361 gctaccgcca atcccaaaac gttgggcacc gaagtagttc ggtacacctt ttgcgcaaat
     9421 atcgatcaga cgttgttcaa cgtcatcgcg attgctcact tcgcgcagaa ccagggtaaa
     9481 ggcgttacct ttcagcgccc ctaaacgcag cttgcgcttg tgccgcgcat actccagcac
     9541 ctggcagcct tccagttgaa aggcgctcag atcgggcatt tccttgcccg gcacgcgagc
     9601 acataaccac tgttccgtta cagcatgttt gtctttttgc ccagcgaagc tgacttcacg
     9661 ggaatgaatt ttcaggaatt tcgccagtgc atccgccaca aaacgggtat tgcagccgtt
     9721 tttgaggatt ctaaccagaa tatgctcacc ttcaccatca ggctcaaagc ccaaatcttc
     9781 caccaccaca aagtcttccg gattggcttt cagcagcccg gtgccttgcg gtttaccgtg
     9841 gaggtaagtg agattatcaa actcaatcat tttgttgcct taatgagtag cgccaccgct
     9901 tcacaggcaa tcccttcccc acgtccggta aatccaagtt tttccgtagt agtggctttc
     9961 acgttaacat catccatatg gcagccgaga tcttcggcaa taaacacgcg catttgtgga
    10021 atgtgcggca acatcttcgg tgcctgagcg atgatagtga catcgacgtt gccaagggta
    10081 taacccttcg cctgaatacg acgccaggct tcgcgtagca gctcgcggct gtcggcacct
    10141 ttaaatgccg gatcggtatc cgggaacagt ttgccgatat cccccagcgc cgccgcgcca
    10201 agcaatgcat cggtcaacgc atgaagcgcc acgtcgccat cagaatgcgc cagcaatcct
    10261 ttttcgtaag gaatgcgtac gccaccaatg ataattgggc cttcaccgcc aaaggcgtgt
    10321 acgtcaaaac cgtgtccaat tcgcattatg tattctcctg atggatggtt cgggtgaggt
    10381 aaaactcggc cagtgccaaa tcttccgggc gcgtgacttt aatgttatcc gcgcggcctt
    10441 cgaccaactg cggatgaaat ccgcaatatt ccagcgccga ggcttcgtcg gtaatggtag
    10501 caccttcatt tagagcgcgc gtcagacagt catgtaacag ctcacgaggg aaaaattgcg
    10561 gcgtcagcgc gtgccataag ccgttgcgat caacggtatg agcaatggca tttttgcccg
    10621 gttcggcacg tttcatagta tcgcgcactg gtgcggcgag gatccctccc gtgcggctgg
    10681 tttcgctcaa cgccaacaat cgcgcgaggt catcctgatg caaacaagga cgagcggcgt
    10741 catgcaccaa tacccactgc acatcgccag cggctttcag acctgccagc acggaatcgg
    10801 cacgctcatc accgccatct acaacggtga tttgcggatg attcgccaga ggaagttgtg
    10861 caaaacggct atcgccagga cttatggcaa tgacgacacg tgtcacccgg ggatgcgcca
    10921 gcagcgcatg caccgagtgt tcaagaatgg tttgattacc gattgagaga tattgcttag
    10981 gacattccgt ttgcattcga cggccaaatc cggccgccgg aaccacggcg caaacatcca
    11041 aatgagtggt tgccatgtta attcccgggc tgatttatcg attgttttgc cccgcagact
    11101 gtgcgcgctt cgacgcgtca ggcaccagac gataaaaagt ttcgcccggc ctggtcatgc
    11161 tgagttcatt acgcgcacgc tcttcgagcg cctcctggcc gccattgaga tcgtcaattt
    11221 cggcaaaaag ttgatcgttt cgcgctttaa gtttcgcgtt tgtagcttgc agtgccgcca
    11281 catcatcatt gacgcgggta tagtcatgta taccgttctt accgaaccac agcgaatact
    11341 gtagccagac cagaatagcc agcaacagca gcgttagttt acccatcctg ccccctgaaa
    11401 aactgcatca tcatcccatg catccgaaga cgactctaca tcctctgttg gggataccgc
    11461 gacaacgcgg gcaaatgtac cacatttgtc cattgttacg tatacccagg gcgtgcagaa
    11521 cataatctca ttattagtta cggtttgaat tatgaacaga ggagacaaga aagtacaaat
    11581 tagcccagta gccacataaa cagtgcgcca aacataatgc ctactgtcac cagggtgaaa
    11641 acaatactgt agcgtagctt tccgtccatc aatgaatgca gcgcaatccc caccactacc
    11701 gcgacaggca tcagtgccag aaagaaaggc caggtgtaga taaagaagaa cagcgtgtta
    11761 gagccataaa tcaacatcgg catcgccagc gcaaataacc aggagataaa accgaccacg
    11821 gcaccaggca gtgaccatgt agtttcttca tcctcagtaa ggctgtcgtt atttgttagt
    11881 gtaatgttat ggctattacg catatttgat cctgttactt tgacgaaccg ggcattgaaa
    11941 cccggtggtg tctcaggatc tgataatatc gttctgtctc aacagatcta ataattgctg
    12001 taccaaattt gttactaatt gttcaccatt gagatgaatt tctgccgatt caggctcttc
    12061 gtaaacggaa tctattcccg taaagttgcg cagttcaccg gcacgcgctt tcttatataa
    12121 gccttttgga tcgcgggctt cgcaaatcgc cagcggcgta tcgacaaaca cttcaataaa
    12181 gcgcccttct cctacgcgtt cgcgaaccat ctggcgttcg gcgcggtgtg gcgagataaa
    12241 tgcggtcagc accaccagtc cggctgcaac catcaaattc gccacttcac cgacgcgacg
    12301 gatattctct ttacgatcgg catcgctaaa accgagatcg ctgcataatc cgtggcgaac
    12361 attgtcgcca tccagcagat acgtactgac gccgagttta tgtaacgcct cctccagcgc
    12421 cccggcgacc gttgatttac cggacccgga gaggccggta aaccacagca ctacaccacg
    12481 atgaccgtgg tgtagttcgc gttgttgcac agtgaccgga tgactatgcc agacgacgtt
    12541 ttcgtcatgc agcgccatta tttatccccc agcaaatcgc gcgcgcccca gtgcggaaag
    12601 tggcgacgaa ccagggcatt caattccagt tcgaatgcac tgaattcaga tgacgcagct
    12661 gttgcctggc taactggctc gtgcaccata ccggcaccca cggtcacatt gctcaggcga
    12721 tcgataaaaa tcagcccacc ggtaaccgga ttttgctgat agcgatctaa caccagtggc
    12781 tcgtcaaaag tgagatccac caggccgatg ccgttcagcg gcaggttttc aacttcgcgt
    12841 tgggtaaggt tattaatatc aacctgataa cgaatgccat caacacgagc acgcgtcttc
    12901 ttaccggcaa ttttgatgtc ataactctgg cccggggaaa gcggctgttc cgccatccat
    12961 accacatcca ccgacgcact ctgcaccgcc ggtaacgctt cgtctgccgc cagcagcaga
    13021 tcaccacggc tgatatcgat ctcatccgtc agcaccaggg tgatcgcttc tccggcaaag
    13081 acttcttcgc gatcaccatc aaaagtcacg atccgcgcga cgtttgattc cacaccagag
    13141 ggcagcactt ttacacgttg cccgacttcc acgcgaccgg atgccagcat tccggcgtag
    13201 ccacgaaaat cgagatttgg gcggttaacg tactgcaccg ggaagcgcat tggctgggca
    13261 tccaccactc gctggatctc cacggtttcc agcacttcga gcagtgtcag accgctgtac
    13321 cacggcatac tttcactttg cgaagccacg ttgtcacctt ccagtgcgga gagcggcaca
    13381 aagcggatat ccagattacc cggcagctgc ccggcaaagg tcagataatc ttcacgaata
    13441 cgggtgaacg tctcttcact gtaatccacc agatccattt tgttgatcgc cacgaccaga
    13501 tgtttgatcc ccaacagtgt ggagataaaa ctgtgacgac gggtttgatc gagcacgcct
    13561 ttacgggcat caatcagtaa gatcgccagt tcacatgtcg atgcgccagt cgccatattg
    13621 cgagtgtact gctcgtgccc tggggtgtcg gcgataataa atttacgctt ctcggtagag
    13681 aaatagcggt aggccacatc aatagtgatg ccctgttcgc gctcagcttg caggccgtcc
    13741 accagcagag ccagatccag cttttcgccc tgggtgccgt gacgcttact gtcgttatgc
    13801 agcgatgaga gctgatcttc gtagatttgg cgggtatcgt gcagcagacg accaatcagg
    13861 gtacttttgc cgtcatcgac gctaccacag gtcagaaaac gcagcaggct tttatgttgt
    13921 tgcgcaatca tccaggcttc gacgccgcct tcattggcga tttgttgtgc aagtacggtg
    13981 ttcatcttaa aaatacccct gacgtttttt cagctccatg gatcccgcct ggtcgcggtc
    14041 aatcacgcga ccctgacgtt cactggtggt ggaaaccagc atctcttcga tgatttccgg
    14101 cagtgtttgt gcatttgact ccaccgcacc ggtcagcggc cagcagccca gcgtacggaa
    14161 acgcaccatc cgttttttga tcacttcgcc cggttgcagg tcgatacggt tgtcatcaat
    14221 catcatcaac ataccgtcgc gttccagaac cggacgttcc gcagcgagat acagcggcac
    14281 aatgtcgata ttttccagcc agatgtattg ccagatatcc tgctcggtcc agttagagag
    14341 cgggaagacg cggatgcttt cgcctttgtt aatttgcccg ttgtagttgt gccacagctc
    14401 cgggcgctga tttttcggat cccagcgatg gaagcggtca cggaaagagt agattcgctc
    14461 tttagcgcgg gatttctctt cgtcacggcg cgcaccaccg aaggcggcat caaaaccgta
    14521 tttgttcagc gcctgtttca ggccttcagt tttcataata tcggtatgtt tcgcgctgcc
    14581 gtgcacgaat ggattaatcc ccatcgccac gccttccggg tttttatgca ccagcagctc
    14641 gcagccgtag gctttcgccg tacgatcgcg gaactcatac atctcgcgga atttccagcc
    14701 ggtatcgaca tgcagcaacg ggaaaggcag cgtacctgga taaaacgcct tgcgcgccag
    14761 atgcagcatg acgctggaat ctttaccgat agagtagagc atcaccggat ttgagaattc
    14821 tgccgccacc tcgcgaataa tgtggatgct ttccgcctcc agttgccgca ggtgagtaag
    14881 tcgtttttga tccataaccg ttcctttgca ataccgctat tttcttgcca tcagatgttt
    14941 cgactatagg gagcgtaaga gaacgaatga aattaccaat tagaatgagt agttccttaa
    15001 cggaataacg atttggcaaa gctaatatca aaaagtgctt aaggcaccgg atttcgggcg
    15061 tttaggaaga tttgaaattg ttttagcgca gcggcagttt catactatgg cggtaaaaaa
    15121 atttgcatgg tatttaagga ctcactatgt tttccgcatt gcgccaccgt accgctgccc
    15181 tggcgctcgg cgtatgcttt attctccccg tacacgcctc gtcacctaaa cctggcgatt
    15241 ttgctaatac ccaggcacga catattgcta ctttctttcc gggacgcatg accggaactc
    15301 cagcagaaat gttatctgcc gattatattc gccaacagtt tcagcaaatg ggttatcgca
    15361 gtgatattcg gacatttaat agtcggtata tttataccgc ccgcgataat cgcaagaact
    15421 ggcataacgt gacaggaagt acggtgattg ccgctcatga aggcaaagcg ccgcagcaga
    15481 tcatcattat ggcgcatctg gatacttacg ccccgctgag cgatgctgac gcagatgcca
    15541 atctcggcgg actgacgtta caaggaatag atgataacgc cgcaggttta ggtgtcatgc
    15601 tggaactggc agaacgcctg aaaaatacgc ctaccgagta tggtattcga tttgtggcga
    15661 ccagcggcga agaggaagga aaattaggcg ctgagaattt actcaagcgg atgagtgaca
    15721 ccgaaaagaa aaatacgctg ctggtgatta atctcgataa cttaattgtt ggcgataaat
    15781 tgtatttcaa cagcggtgta aaaacccctg aagcagtaag gaaattaacg cgcgacaggg
    15841 cgctggcaat tgcacgcagt cacggaatag ccgcaacgac caatccgggt ttgaataaaa
    15901 attatccgaa aggcactggg tgttgtaatg acgcagaaat attcgacaaa gcgggcattg
    15961 ctgtactttc ggtggaagcg actaactgga atcttggaaa taaggatggt tatcagcaac
    16021 gcgcaaaaac acctgccttc ccggcgggaa atagctggca tgacgtaaga ctggataatc
    16081 aacaacatat tgataaggca cttccaggaa gaatagaacg tcgctgccgt gacgttatgc
    16141 ggataatgct accgttggtg aaggagctgg cgaaggcgtc ttgatgggtt tgaaaatggg
    16201 agctgggagt tctaccgcag gggcggggaa ttctaagtga tacccatcat cgcatccagt
    16261 gcgtccggtt tacttccggg aaaaccgcac cgtattgcgc cggtttatct ccgctggagc
    16321 ggggaacacc tctggcgaat agaaagcaag ccaggttttg accagtcgat cgtacatggc
    16381 attggccgtt gccattgttg gcaggccaaa aaacagcccc tgagcgttac ccgcagccat
    16441 caacctgtgc gccaggataa gcgccgcttc tgttttacct gcgccggtca catcttccag
    16501 aataaacaac tgtggtcctg gctgattgat atccagatcc agcacctttt gctgtaacgg
    16561 tgtcgtattc tcaataaaag gaaacagcgt attaattccg gtgaaaggcg cggtttctgc
    16621 ttgtggcgga aagacggtta atgcgttttg agcctgaact aaagcctttt gccagtaatc
    16681 ttcaatatct attgcctgtg cgacgcgtgg aaaaaatcgc gttgacgacc ctgtccagtc
    16741 tgcaagtacg acggttgcag agatatacca ggaaagttgt tttataagtt caacgccctc
    16801 gtcatcatcc cagaatgtgg ggatctctat tcgcggaaac agtggcttga tttcaaggag
    16861 aaaatctcgt gctgcagctt tgtcttcagg cagaaaatta tccaattcat ccatacgatc
    16921 agggggccga ccatgatgcc ctgtcgtcac gggcatccac atttctatca ctcgcctaag
    16981 tttgcgcgga gagagcgaag atgaaggaag taactcctga cattcactta aataatgatt
    17041 ccacaaccag tagcccagcg ttgaatgaga gattttttcg taattttttc tggcaccttc
    17101 ctgaatcttg aattcagggg gcaggtaaag ttgttgaaaa gagcgggcaa attttccaat
    17161 atcgtgccag cacagcaacc aggcgaaaaa ttgagctgcc tgttccttgt cagaaatccc
    17221 taattgacga aaataatcag ccagcccgaa gcaatttctt ttaaccatta aatagcccat
    17281 tgcggccaca tccagcaaat gccagcaaag aaggtgatag tcgtcgctac cctctttctc
    17341 gccacgtcgg ttttttcccc agaaatcaaa gaaagtcaca atatttttat ccttcagtaa
    17401 acttaaagga tatttacgca tattttaaaa actatctgtg atatatatca gccaataaaa
    17461 catttacatt atgaataaat taatgatttt catttgaaat tcataataat aaatagagac
    17521 tatatattca cataaaaaat ataaatttta aaaaagggtg caggagacca aaccatcaca
    17581 attcagtacg cggggtttta cccccgcgtg gtggctactt cttcgattcg taagcgagaa
    17641 ctgccttaaa ctccatacca cgttctctga tactcaactc acataacgag tcacctacca
    17701 tcaacgtgaa tcccagtact gtacaacaca gtacgatgat tgccacaagg gcatattttg
    17761 tcagcatgtc ttgcctccct gcaaagaaga gactaacatc cgaattgcta cgttcgaacg
    17821 cgagcctcag gttgatagaa atatcgcctg gggcttttgt ccatctggaa cctcgcgaat
    17881 gcttaacgcc agacagcctc aagcacccaa cgccattcta tacctgttaa ttttccctgc
    17941 gatgttcacc tttaaatgcc ggaatgctgc tcgcaaaata aaaacgcccc catcatttct
    18001 gacagaggcg tttaatttat ccggcaatat ttacccttcg tgtaatccac attccctttt
    18061 taagccaaag aaacgcgttt cttcttccac catgccgggt tcccatttac gggttgtatg
    18121 ggtatcgccc accgataaat atccttcgtc ccataatggg tgatatttca ggccatgttt
    18181 ttgcaggtac tgataaatag ttcggttatc ccagtcgata atcggcagca ctttaaatac
    18241 gccacgctga attgccagca ccggtaaatt ggcacggctg ccggattgtt cacgccgcag
    18301 gccagcaaac cacgtttgcg cattcagttc tttcagcgcc cggttcatcg gttcgacttt
    18361 gttgatgtca ttgtactttt caatgccttc aacgccctgt tcccacagtt tgccgtagcg
    18421 tgcttcctgc caggcggcgc tttcggtagc gcggtacact ttcaggttga gcttgagttt
    18481 gtccgttaac tcgtcaataa agcggtaggt ttccgggaac agatagccgg tgtcggtgag
    18541 gatcaccgga atatccgggt gaatctgatt caccagatgc aggctcaccg ccgcctgaat
    18601 gccaaagctg gaagaaagta catattcacc gggcagatta tccagcgccc aggcaacgcg
    18661 gccttcagcg tccagttttt ccagttcggc gttagtttct gccagcgcca gaatgcgatc
    18721 taccttcggc agttcgttca gggcgtttag atcgagtttg gacatagatt cctcactgtt
    18781 tgccttgcct gatgcgacgc tcacgcgtct tatcaggcct acaagaccgg gctgatggtt
    18841 aatcccacaa atcgcgcgcc ggatcgagca ccgggcgaat gatgcccgca cgcaccgtaa
    18901 agtcgccgaa gccttcaccc gcttcgcgct ctttcgccca gcgccctatc agttcatcaa
    18961 gcgacgccag gatttccggc tcggtgatgt tttctttata catccgtggg atacgtgtcc
    19021 caatgcggtt gccgccaaga tgcaggttgt agcgacccgg tgctttaccc accaggccca
    19081 cttccgccag catcgcgcga ccacaaccgt tcgggcagcc tgttacacgc atcacgatat
    19141 gctcatcgct gacaccatgt ttcgccatta aattatcgat gttgtcgata aaagacggca
    19201 ggaaacgctc cgcttccgcc atcgccagcg ggcaagtcgg gaatgacacg caggccatcg
    19261 agttttcacg ctgcggcgtg acggcattca ttaacccgct ctctttggca attttctcga
    19321 tcttcgcttt ctcgctttcc ggcacaccgg cgatgatcag attctggttc gccgtaatgc
    19381 ggaaatcacc tttgtggatc ttcgcgatct ccagcaggcc agttttcagc ggacgccccg
    19441 gataatcaag gatgcgacca ttttcgataa acagcgtcag gtgccagtta tcatcaatgc
    19501 ccttaaccca gccaatacga tcgccgcgtc ctgtaaattc atacgggcgg atcggttcaa
    19561 atttgatccc cgcgcgacgc tccacttccg ctttaaacgt ctcaaccccc acgcgctcca
    19621 gtgtgtattt tgttttggca tttttacgat cggttcggtt accccagtca cgctgagttg
    19681 tcacgacggc ttccgccacc gctagcgtat gctctagcgg cagatagcca aactcactcg
    19741 ccgtgcgggc gtaggttttc ttgttaccgt gttcgataga aagcccaccg cccaccagca
    19801 ggttgaagcc caccagcttg ccgttttccg cgatcgccac gaagttcatg tcgttggcgt
    19861 gcagatcaat atcgttctgc ggcgggatca ctaccgtggt tttgaactta cgcggcaggt
    19921 aggtctgacc aaggatcggt tcttcatcgg tggtggcgac tttttcctga tcgagccaga
    19981 tctccgcata cgcgcgggta cgcggcagca gatgttcaga aatcttcttc gcccactcgt
    20041 acgcttctgc gtgcagctgc gactcgtaag ggttcgaggt gcagagtacg ttacggttca
    20101 tgtcgttagc agtcgccagc gcatcaagac cgaccgagtg cagcatctgg tgcaccggtt
    20161 tgacgttctt tttcagaatg ccgtggaact gaaacgtctg gcggttggtc aggcgaatgc
    20221 tgccatagat ggtgttttca ccggcaaatt tatcgatcgc ctgccactgt ttggtggtga
    20281 taactccacc cggcagacgg cagcgcaaca gcatcgcgtg gcgcggctcc agcttctgtt
    20341 cagcacgttc ggcgcggatg tcgcggtcat cctgctgata catgccgtgg aagcggatca
    20401 gcaggaagtt atcgccctta aagccgccgg tcagaccgtc gtttaaatct tccgcaatgg
    20461 tgccgcgcag gtagttgctt tcaagcttca tgcgctcggc gtctgtcagt tttccttcga
    20521 ccactaaagg ccctggatgt ttttcgctca ttagtagaca tctcgctgat aacggcgctc
    20581 tacgcgcagc tcacttaaaa attcatccgc cgcttcggtg tccatgccac caaattcggc
    20641 aatcacttcc agaagtgcct gctcaacgtc tttcgccatg cgattagcgt cgccgcagac
    20701 ataaatgtgg gcaccgtcat tgatccagcg ccacagctcc gcgccctgtt cgcgcagttt
    20761 atcttgtacg taaacttttt ctttttggtc gcgcgaccag gcgagatcga tccgcgtcag
    20821 cacaccctct ttgacgtagc gctgccactc cacctgatac aggaagtctt ccgtaaaatg
    20881 cggattgcca aagaacagcc agtttttacc tggcgcttcg tcagcggcgc gttgctgcat
    20941 aaaggcgcgg aacggcgcaa taccggtgcc tgggccaatc ataatcaccg gggtttccag
    21001 gttagcgggc aggcggaagt tatcgttatg ttcgataaat acgcggactt cgccctcttc
    21061 ttccacgcgg tcagcgagga agctggaggc accaccggca cgggcgcggc cttccacgtc
    21121 gtaacgcacc acaccaacgg tgacgtgtac ttcgttctcg acttccgcct gcgaggaggc
    21181 aatggaatag agacgcgggg tcagcgggcg cagcagatta attagcgctt cggcatcaag
    21241 ctgtgccggg gagaaacgca ccatgtcaac aatcagcgtc gtcgcggcgt aatgctgtaa
    21301 cttcgcttta tcgcccacca gcggcagcag tgtttcactg cgggtaagcg tggcgtaatt
    21361 ctcaacaatg ttggcggtgt tgacggtcag ttcgaagtgc cactgtagcg cttcgttcag
    21421 aggcaacgtt ttgccctcga cagtgacagg ttcatcgcct gtcagccaca gcagttcgac
    21481 aagttctttc accagtgccg gatcgttctg ataccagacg cccagcgcgt cacccggctg
    21541 gtaacgcagg cccgagtcgc ctaagtcaat ttcgatatgg cgaacgtctt tttcagagtt
    21601 acgcccagta attttctggt taacagagag gctagccacc agcggcgcgt ctttgctgta
    21661 cgggctggtg tggatttcat ttaccgcgcc agtagcgacg gattgcgaag gtgccgcgac
    21721 aggcgcacgc gatttaagcg catcaaccac gcgggcgcgc cactcgctgg cagcagcctg
    21781 gtattcaaca tcagaatcga cacggtcgag caggcgttca ccacccagtt ccgccagctt
    21841 gctgtcaaaa tctttcccgg actggcagaa aaattcataa gagctatcgc cgaggctaaa
    21901 cacggcaaac gcggtgtttt ccagctttgg cgcttttttg gagaacagga acttatgcag
    21961 cgcgacggct tcttccggcg gttccccttc cccttgcgtt gacgtcacta cgatgagcag
    22021 tttttcgctg gcgatttgtt tgaatttata gtcgcccgcg ttcaccagct taacgttcag
    22081 ttttgctgct aataaatcat cgcgtaatgc ttcagcaacc cggcgcgcat tgccggtttg
    22141 cgaagcagag ataatcgtta tacccggcat ttctgcggcg ggcgctggcg tcactgcaag
    22201 cgcaacaggc tgctggttga gcacgcccca gaaatagcca gaaacccagg caagctgggt
    22261 aggcgttaaa tcggtcgtgg ccgcctgaag gcgtgccagt tgctccgggt tcaacggaag
    22321 caacgcggaa ggtgggacct gtgtcgtcat gcgtcgttat gttccagtaa gcaaagctgt
    22381 ttctgcgccc tgtcagcgcc cataaaacag aagagaaggt aaggttaacg gggtgaacgg
    22441 tgtggattaa agacgggata gcgataacta ataaccaaat cgactaacct gttttagcaa
    22501 tagtctttaa caacaaaata gattaaccaa cctaatgaaa aacaaatgaa tttagccaat
    22561 cattaagata aatcagcgat tttgcgcaac aaagtcgttt tagataatgc gaaaaaacag
    22621 cctttccggt actctacggc ggttttatcg tcctgtagag aaattatgat gtccaccacg
    22681 ttatttaaag atttcacctt cgaagccgct caccgcttac cacatgtccc ggaagggcat
    22741 aaatgtggtc gcctgcacgg gcattccttt atggtgcgac tggaaattac cggggaagtc
    22801 gatccgcata cgggctggat tatcgatttc gctgaactga aagcggcgtt taagccaact
    22861 tatgagcgcc tagatcacca ttatctcaat gatattccag gtctggaaaa cccaaccagc
    22921 gaagttttag caaaatggat ttgggatcag gttaaacccg ttgtgccgct gttaagtgcg
    22981 gtgatggtaa aagaaacctg caccgcaggg tgtatctatc gcggcgaatg ataagagtgt
    23041 gtcggcggtc aatttccctt tagtaacgct atgttagggt gttgtgttct ggatatctgg
    23101 ggcatgacat ggaagacgac tgcgacatta ttattattgg tgccggtatt gcaggcaccg
    23161 cttgcgcgtt acgctgcgcg cgagcggatt tatccgtttt gttactcgaa cgcgctgaaa
    23221 tccccggcag caaaaatctt tccggcaggc ggttatatac ccatgcactc gcggaactcc
    23281 tcccgcaatt tcatctgacc gcgcctcttg aacgacgcat cactcacgaa agcctttccc
    23341 tgttaacgcc ggatggcgca acgacgtttt ccagcttaca gcccggcggt gaatcctgga
    23401 gtgtattacg tgcacggttc gatccgtggc tggttgccga agccgaaaaa gaaggtgtcg
    23461 aatgcatccc cggagcgacg gtggatgcac tgtatgaaga aaacggcaga gtctgtggcg
    23521 ttatttgtgg tgacgatatt ctccgcgccc gttatgtggt gctggcagaa ggtgccaaca
    23581 gcgtcctggc tgaacgtcac gggttagtga ctcgtcctgc tggcgaagcg atggcgttgg
    23641 ggatcaaaga agtgctgtcg ctggaaacat ccgctattga agaacgtttt catctggaga
    23701 ataacgaagg cgcagcgttg ctgttcagcg gcggaatctg tgatgactta cccggcggcg
    23761 catttcttta tactaatcaa caaacgctct cgttagggat tgtttgcccg ctctcttccc
    23821 ttacgcaaag tcgtgttccg gcaagcgagc tgctgactcg ctttaaagcg catccggcag
    23881 tgcgcacgct tatcaaaaac acggaatcac tggagtatgg tgcgcatctg gtgccagaag
    23941 gtggcttgca cagtatgccg gtgcaatacg ccggtaacgg ctggctgctg gtgggcgatg
    24001 cgttgcgcag ttgcgtcaat accggaattt ccgtgcgcgg catggatatg gcgctgactg
    24061 gcgcacaggc ggcggcacaa acgctaataa gcgcctgcca gcaccgcgag ccgcaaaatc
    24121 tgtttgcgct ttatcatcac aacgtagagc gcagcctgct atgggatgtt ctacaacgtt
    24181 atcagcatgt tccggcgctt ttgcaacgcc ctggctggta tcgggcgtgg cctgcgttaa
    24241 tgcaggatat ttcccgcgat ttatgggatc agggtgataa acctgttcca ccgctgcgcc
    24301 agttattctg gcgtcattta cgtcgtcatg gcctgtggca tctggcgggc gatgttatca
    24361 ggagtctgcg atgtctgtag cccgtaatct ctggcgcgtt gctgatgcgc cgcacattgt
    24421 tccggctgac tccgttgagc gtcagatggt acaacggttg attaacgcct gtccggtagg
    24481 tcttttttct ctcacacagg aaggtggctt acgtattgac tatcgcggct gcctggagtg
    24541 tggcacctgc cgtttgctgt gcgacgaatc cacactacaa cagtggcgct atccgccttc
    24601 cggattcggc atcacctacc gctttggata aataataagg ataatttatg cccctcttac
    24661 acctgctccg ccagaatccg gtgattgctg ccgttaaaga taatgccagc ctgcaactgg
    24721 ccatcgattc tgaatgccag tttatttccg tgttgtacgg caatatctgc accatcagta
    24781 atatcgttaa aaagattaag aacgccggga aatatgcttt tattcatgtt gatttgctgg
    24841 aaggcgcgtc aaataaagaa gtggtgattc agtttttgaa actggtgacc gaggcggatg
    24901 gaattatcag caccaaagca tcgatgctaa aagcggcaag agcggaagtt tttttctgta
    24961 ttcatcgcct gtttattgtt gattcgattt cgtttcacaa cattgataag caagttgcgc
    25021 aatcgaatcc ggattgtatt gagatcctgc caggctgcat gcccaaagtg ctgggctggg
    25081 tgacggaaaa aatccgccaa ccgctgattg ctggcgggct ggtatgcgat gaagaagatg
    25141 cgcgtaatgc gattaacgcg ggtgtcgtgg cgctttccac cacgaatacc ggagtctgga
    25201 cgttagcgaa aaaattgctt tgacgggata atcgtaacca attgaatttg ttttgatttg
    25261 ttggccgcac gccacatccg acattcagcg cctgatgcga cgcttaacgc gtcttatcag
    25321 gcctacaggt ccccagtcca gaaccgtagg caggatcatc cggcagcacg ttattggtat
    25381 tctgcgtgaa tgtttgtcac cagtgcctcc agcaccactt tccagtcatc aaccacgtca
    25441 atatctgcct gtgaaaatac cgctgcgctg gcgtcatgat taatcgccac cataaacttg
    25501 ctattgcgta cgcccgccat taacgctgct gcgccggaag cgccaacaac aatgcacacc
    25561 tcaggtgcca gcagatgtcc ggaaatacca atcacttttt cagcatcaac gccaccgttc
    25621 atcactcgcg cccgactgta gcccacttcg gttcccagct tttctgccag cataacgatt
    25681 tcctggttat ctgcctctcc gccctgcccc accaccagca cccgtctggc ctcagctaat
    25741 gggtcgcggc taacattttt cagatcctct gtgctaacca gccagtcggg aagcgcacct
    25801 gggacaatgt tcagttgctg cattccagag ggcaacgtgg cgtttttcgc tgctcccgcc
    25861 tgacgcgcca gggaaaggca taacgggcgt ttttctgttt gcagcgtcgc cgttaacgca
    25921 tttccccagt gggatttgcg catgcttacg gttggaatat ctaacgaagt cacctgacag
    25981 atgcttgcac catgtaaacg ccaggccagc cgggtcgaca gttcatcgcc aaacgttccc
    26041 ggcggaaaga gaacaacgtc cgcaggcgtt cgctgccact gttccaccat cgcatccagc
    26101 acctgttccg ccactacggg ctgaggttct atttgccagt gcgccagcgt acaaccgctg
    26161 aaatcctgcg ccgccagcca gctcgctatt gcggcattct cctgatttat tgtcacaatt
    26221 gcgatattca tggctgcatc ctttggcgta aatagtcctg ccatagcttt tgagcttttt
    26281 ctgctaccgt ttgcccatca atcagcgtcg ctccgcatcg ttgctctgct cttgccagtt
    26341 gcagacactg catcgctggc atttcggcag caacagtttt acggataatt tctgctttcc
    26401 ccgcagccat tcgctggcgc attcccggta ctggcaaggc cacttcaccg cactgacgca
    26461 cggcaatcac cgcaggcaag cgaacccggc aacagcgcaa cccatgttca gtacgctgtt
    26521 cgagggtgat aaacagcgcg tcgagagtga aacgctccac cagggtgaag cagggccagc
    26581 ccagcatttc cgccagcaaa aatggcgttt gcccattctg cccttcgctg ctttggcagc
    26641 cggtaattat cagatccagc ggattctgac gctgccattc ggcaatatgg cgagcgacaa
    26701 attccggcgc aaagcgcaga tctgccgccg tctccagcaa tacagcttcc tcaaacccaa
    26761 gagccatgag atagcgcagc cagtgcaacg cccgttcatc ccccatgctc aacgcggtta
    26821 aagacatcgg cgtaccgttt tttctctgcg ctaacagcag cgcggcagcg gcctgttcat
    26881 cagcaccgag taaacttcgc agtagcgaaa catccggtcc gctattaccc tgagccgccg
    26941 cctgccactc tttttccgcc agcattccgg catccggttc ggctttaaac gctaacagaa
    27001 tgttcattcg cacctcctac atttcccgcc gccaccagcg ggagggcttt agtttctggt
    27061 gcccatagcc aggtcacaac aaaaccaacc aacaacactg tcgccaggag caataaggtg
    27121 acttgcattc cccactgcgc cagcacccac ggcagcaggc cagtacttac cgccgcgcca
    27181 agtcgactca tggcggtggc aaaaccgacg cccagcgagc gaatgtcagt aggaaaactt
    27241 tccgcaggca aaatgcccac cagattactg actgccgaaa tggtggtgct gaagagaaca
    27301 aaaagcagca gcgttaatga actgccggaa ggcaaacagg ccattactac cagcgttgcc
    27361 gccagcagca aaaaacttcc cagcaaaaat ttgcgatgtg ccagcaggtg cgtcagaact
    27421 aatcccagca gcgcgcccac aattaacaac gcattaagca tcaggctggc agtcagcgca
    27481 tcttccagac caatagtctg ggcgatagtt ggcagccagg tataaatcac aaaccatggg
    27541 attacgaggc agacaaagaa gatgctgtta aacgccgtgc gccgccagta acgcgaagag
    27601 aacaaggttt tgatgtgttt atgggtcgcc gttaccactt catcgcccag taaaacatgg
    27661 ggaccaaaat agcgatgcac gatagcgtga gcttctgcaa aacgcccctg gcacagtagc
    27721 caacgtggcg attctggcgt tccccagcgt aataacgtaa tcaacaacgc gggcagagct
    27781 gccgatgcca gcaaccagcg ccaggcctcc gggttttcgg aaataaagtg atgtccggca
    27841 atacttgcca acacatagcc tacggtccac accacgctga atgcgcccag caaaataccg
    27901 cgatggcggc gcggggaaaa ttcagccagc aaggtgtgac ctactgagta atcgcctccc
    27961 agaccaatgc cgatcaaaat gcgcagccca ataagatgct ctggcgtggt ggcaaaaaat
    28021 tgcaagaacg aagcgagcgt aatcagcaaa aagctgaagg tgaagatttt ttgccgacca
    28081 atatggtcgg agatccaccc aagaaccagg ctgccaagaa acaaaccaag gagcgccgag
    28141 ccgccgctca ttcccgccat aaacggcgtc agttgcatgg cgggcgtaag ttgaataatg
    28201 gcgtaaccaa tgacgccgag aacataaccg tcggtcaggt gtgcgccgaa agtgagcgca
    28261 gcaattcgac agtgaaaacg gttaagcggt aaatcatcca ttcgcaccgg tgaagtgttc
    28321 atctttaccc cttcattgtc cggtaataag aacggcttct cgcctgagaa gccgaaaagg
    28381 caacgccccg tcatcgcgga acgctgcctg atacattatt tttcaatcgg atagatagta
    28441 cccgtattca taatgccgtt aggatcgaac tgctttttca gcccttccag caacgcccac
    28501 gcgctgccgt gttccagctt gctccagtga acgcgatgtt taccaatgcc atggtggtgc
    28561 accatcgaac cgccgaggcg aatggtttct tcgcagatga ttttgttgag cggattgtgg
    28621 tacttgtcga tttcctcttc cggcttacag tcaacaacgt tgtagtcata gacgaagtac
    28681 atgttggtgc cgttctgata gctatgagag gagtgaccgc ccagcatggt gatgtcgtcg
    28741 gcgtgcggga actcagtacg aatacggtta ataacgcttt cgtagatttc gtggatgcag
    28801 ctccagcagc cggacacttc ggtggtaaag cccatgttgc cggttttgag gatctgcaca
    28861 cgttcggcag ccactttatc cggtccccag ttcaggttgt taaaccaggt ttcaatcagt
    28921 ttgctgtcca cgcgctggca ctgcgggtag cgggcaacga tttccgcaat cccttcgccc
    28981 gtcgccttcg caatgcgagg gttaccttca gccataaaga tcagcaccca ttttccgtcg
    29041 gcaaaatggg tgaagtgttg ggtgccatct tcagcgtcat acaaacgagc gatcgacgga
    29101 cgataccctt ccaccatgat ttcacgcagg atgttgaagc cggttttcat gtcttccagg
    29161 atatagccgt agaagaggtt gttttccggg gtgtatttaa agattttcac tgttacttca
    29221 gtgatatagc acaatgcacc ttcgttgccg atgatgatgt gacgaatgtc cgggccagcc
    29281 gcgcggcgtg gcacgttttt aatgcgtgtg acggtgccat ctgccaacac tgcctccaga
    29341 ccaacgacca tatcttcgat tgcgccgtag agtgtggaga actgcccgat actgcgggtt
    29401 gctaccaggc cgcccatctg cgccagcggc tttgactgcg gagaatgccc cgtggtgtaa
    29461 cctttttcac gcaacgcgtt ttccagcact tccagcggaa caccacattg cgccgtcgcc
    29521 tgcatattct caatatcaat attaatgatt tgattcatgg cggagccgtc gagcaccacc
    29581 gagttttcta caacagtttc cagcccacct tcggtggcgg aagcaccggt acgcggcaca
    29641 ccgttaattt tgtgcgcatt cataaaattc agcacacggg atacttactc tgtggaaccg
    29701 agttttacga ccgctgccgg aatcggcaaa gtataaatgc catgaatatc cggaaattta
    29761 cgaaaacggt caatactgtt tttctttaat actgtttcat cggtaattac gcgatctgca
    29821 ccaacaattt cctttagctg gtcgacaatc gctgcgcgag ataaagacat agtaattcct
    29881 tcctgataat aaaaatggaa taatcattaa taagagaaaa cagaaaccac cgttatggat
    29941 tagcgcacta aataaccgcc atcgaccact aataaatgcc cgttgacata attcgatgcc
    30001 tgacttgcga gaaacacggc tgcgcccatt aaatcctgag tatcgcccca acggtttgcc
    30061 ggaatatgat caagaacgcg ctgattggtt tctggattac tgcgtgtcgc cagcataata
    30121 tgctgttgca taatagccag gggcgatacc atttacctga atattatatt gacctagttc
    30181 atcacaataa gctttggtga acccggcaag agcatgttta gtggcagaat atgcaggtga
    30241 ccattgtcca cctaagtaag agaacaatga acagatatta ataattttgc cgcttttctg
    30301 cgggatcata atttttgccg cttcatagct taactcgaat gcggcggtca ggttcacatc
    30361 aatcatcgga tcccagtcgg cacgaccgaa gtccagcacc ttattcagct tacaaatacc
    30421 ggcattgtta accagaatat cgacagtacc gaagcgctca cagcaggcag cgataatctt
    30481 ctgcggtgcg ccttctgcgg taatatccac ctgcatgaag tccacttcaa caccctgttt
    30541 ttcaatcatt tcctttgttt cgccgttatc tttgacgaag ctaggaataa aaatatttgc
    30601 gccagctttg gccaacgcca ttgcaaatgc ctgacctaaa ccgctattcc cgccggtaac
    30661 aattgctgtt ttacctttca gggagaaaaa atccattgag aacgcattga gagattcgat
    30721 tgacatagtt agctccaatt tccgtaaggc aaaaaaaaag aaaggtaact tcccccgcga
    30781 gagggaagtt acctttctca ttatctctgg taactggaga taatttttag cataacggca
    30841 taactgctaa tgtgatcgta atcacagtgt gatattcgta ttgtcaaata gttttaaaaa
    30901 gcagaagcgt gattactctc acacaacacc tctgctttcc atgttatttc ttattgcaat
    30961 tactagagac ctgagaaaag taatccacct gaaagggcgg cttacttttc tctttttttt
    31021 gtctattaca aatgggaata cgatatgcaa cacaactcat atcgccgttg gataaccctc
    31081 gcgataatta gttttagcgg cggcgttagt tttgacctgg cttatttacg ttatatttat
    31141 caaattccca tggcgaaatt tatgggattc agcaataccg agataggttt aataatgagt
    31201 acctttggta ttgcggctat tattctttat gcccccagcg gcgttattgc cgataaattt
    31261 tcacaccgca aaatgattac ttccgcgatg atcattaccg gattactggg tctgttaatg
    31321 gcaacgtatc caccgctgtg ggtaatgctc tgtattcagg tcgcctttgc gataacgacg
    31381 attttaatgc tgtggtcggt gtcgattaaa gccgcctcgt tgcttggcga tcatagcgag
    31441 caagggaaaa ttatgggctg gatggaaggg ctgcgcggcg tcggtgtaat gtcgctggcg
    31501 gtgtttacca tgtgggtctt ttctcgcttt gcaccggatg acagcgccag cctgaaaaca
    31561 gtcattatca tctacagtgt ggtttacatc ttgttgggga ttctgtgctg gttttttgtt
    31621 agcgataaca acaacctgcg cagtgccaat aacgaagaaa aacagtcatt ccagcttagc
    31681 gacatccttg ccgttttgcg tatcagcacc acctggtatt gcagcatggt gatttttggt
    31741 gtcttcacaa tctacgccat tctgagttac tccaccaact atctgaccga aatgtacggc
    31801 atgtcgctgg tggcagcgag ctacatgggg attgtgatca acaaaatctt ccgcgcgctg
    31861 tgcggcccac ttggaggcat tatcaccacc tacagtaaag tgaaatcccc tacccgcgtg
    31921 attcaaatcc tttccatcat cggtttgctg gcgttgacag ccctgctcgt cacgaactct
    31981 aacccgcaat cggtcgcgat ggggattggc ctgattttac tgctgggatt cacctgttac
    32041 gcctcacgcg ggctgtactg ggcctgcccc ggcgaagcga gaacaccgtc ttacattatg
    32101 ggcaccacgg taggtatttg ttcggtgatt ggattcctgc cggatgtctt cgtttacccg
    32161 attatcggcc actggcaaga caccctgccc gctgcagaag cctaccgcaa tatgtggctg
    32221 atgggcatgg cggcgcttgc catggtgata gtctttacct ttttgctgtt ccaaaaaatt
    32281 cgtactgctg atagcgcccc cgcaatggct agcagcaagt aagccaggaa aattacagga
    32341 gaaaaatcat gtcgaaaaaa tacatcatag ggattgatgg cggaagtcag agcacaaaag
    32401 tggtgatata cgatctggaa ggtaatgtgg tttgcgaagg gaaaggctta ttgcagccga
    32461 tgcacacgcc agatgccgat actgcagaac atcctgacga cgatttatgg gcatcattat
    32521 gttttgccgg tcacgatttg atgagtcagt ttgccgggaa taaagaagat attgtcggta
    32581 ttggtctggg atccatccgt tgctgccgtg cgttattgaa agccgatggc acggccgttg
    32641 attagctggc aggatgcacg cgtaacgcgt ccctacgaac acactaatcc tgacgtggca
    32701 tatgtcacct ctttttcggg ttatctgacg catcgcttaa ccggcgagtt taaagacaat
    32761 atcgccaact attttggtca gtggccggtg gattataaga gctgggcatg gagcgaagat
    32821 gctgcggtaa tggagaagtt taatatcccc cgtcatatac tgtttgatgt gcaaatgcca
    32881 ggcactgttc ttgggcatat cacaccacaa gccgcgctgg cgacacattt cccagctggg
    32941 ctgccggttg tttgtaccac cagtgataaa ccggtagaag ctctgggggc cggattactg
    33001 gatgatgaaa cggcggcgat ttcactcggt acttatatcg cgctgatgat gaacggcaaa
    33061 gccttgccga aagatccggt ggcgtactgg ccgattatgt cttctattcc gcaaacattg
    33121 ctgtatgaag gttacggtat tcgcaaaggt atgtggacgg tgagctggct gcgcgacatg
    33181 ttaggcgagt cgttaattca ggatgccagg gcgcaggatc tttcaccgga agatttactc
    33241 aacaagaaag cctctagtgt gccacctggc tgtaatgggc tgatgacggt gctggattgg
    33301 ctgaccaatc cgtgggaacc gtacaaacgc gggattatga tcggctttga ttccagcatg
    33361 gattacgcat ggatatatcg ttcgatactg gaaagcgtgg cgctgacgct gaagaacaat
    33421 tacgacaata tgtgtaatga aatgaatcac tttgcgaagc atgtgatcat tactggcggc
    33481 ggttcgaaca gcgatctgtt tatgcagatt tttgccgacg tgttcaacct tccggcacga
    33541 cgtaacgcca ttaacggttg tgcaagcctg ggggcagcga ttaatacagc ggtaggtctg
    33601 gggctatacc cggattacgc aacggctgtc gacaaaatgg ttcgcgtgaa agatatcttt
    33661 atgcctgttg agagcaatgc caaacgctac gacgcgatga ataaaggtat tttcaaagac
    33721 ctaaccaaac atactgatgt gatcctgaaa aaatcgtatg aagtgatgca tggggaattg
    33781 gggaatgcgg attcgatcca gagctggtcg aatgcgtaag tgaggaagac cgggcgggaa
    33841 actgcccggc ctgaatatac ctgaatggtt atccccgctg acgcggggaa ctcgaataac
    33901 tgaccaacgt ctggctctct aaatccggtt tatccccgct ggcgcgggga acactctaaa
    33961 cataacctat tattaattaa tgatttttta agccagtcac aatctaccaa ctttacagga
    34021 tcacacaaac aacacatcca ttatgttaaa gagcacttaa tccattgatt aaaaaggtaa
    34081 atatttaaaa taactctata caaactaaaa tctaccaaac ttcgccgcaa taattttcac
    34141 tccagcgaaa aattaacgcc gcagaatttg tagagaatga taatgattag tccgggatgg
    34201 gcaagatgga tgagttttca ggggcgacaa ataatacaaa tgagtggaat tttttacaag
    34261 ttccac
//
LOCUS       DAJVQH010000028        33594 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJVQH010000028 DAJVQH010000000
VERSION     DAJVQH010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.028"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            535..672
                     /locus_tag="OQM87_001460"
     CDS             535..672
                     /locus_tag="OQM87_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507642.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1272..2051
                     /gene="ipaJ"
                     /locus_tag="OQM87_001461"
     CDS             1272..2051
                     /gene="ipaJ"
                     /locus_tag="OQM87_001461"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCS1507643.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2999..3928)
                     /gene="virB"
                     /locus_tag="OQM87_001462"
     CDS             complement(2999..3928)
                     /gene="virB"
                     /locus_tag="OQM87_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCS1507644.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4145..4381)
                     /locus_tag="OQM87_001463"
     CDS             complement(4145..4381)
                     /locus_tag="OQM87_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1507645.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="OQM87_001464"
     CDS             complement(4385..6286)
                     /gene="ipaA"
                     /locus_tag="OQM87_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCS1507646.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTSPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="OQM87_001465"
     CDS             complement(6295..7293)
                     /gene="ipaD"
                     /locus_tag="OQM87_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCS1507647.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="OQM87_001466"
     CDS             complement(7344..8435)
                     /gene="ipaC"
                     /locus_tag="OQM87_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCS1507648.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="OQM87_001467"
     CDS             complement(8455..10197)
                     /gene="ipaB"
                     /locus_tag="OQM87_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCS1507649.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINKTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="OQM87_001468"
     CDS             complement(10203..10670)
                     /gene="ipgC"
                     /locus_tag="OQM87_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCS1507650.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10727..11353)
                     /gene="ipgB1"
                     /locus_tag="OQM87_001469"
     CDS             complement(10727..11353)
                     /gene="ipgB1"
                     /locus_tag="OQM87_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCS1507651.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="OQM87_001470"
     CDS             complement(11369..11758)
                     /gene="ipgA"
                     /locus_tag="OQM87_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCS1507652.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="OQM87_001471"
     CDS             complement(11771..13255)
                     /gene="icsB"
                     /locus_tag="OQM87_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCS1507653.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13569..15185
                     /locus_tag="OQM87_001472"
     CDS             13569..15185
                     /locus_tag="OQM87_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCS1507654.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLPQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15195..15557
                     /gene="ipgE"
                     /locus_tag="OQM87_001473"
     CDS             15195..15557
                     /gene="ipgE"
                     /locus_tag="OQM87_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCS1507655.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15557..16015
                     /gene="ipgF"
                     /locus_tag="OQM87_001474"
     CDS             15557..16015
                     /gene="ipgF"
                     /locus_tag="OQM87_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCS1507656.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16024..17139
                     /gene="mxiG"
                     /locus_tag="OQM87_001475"
     CDS             16024..17139
                     /gene="mxiG"
                     /locus_tag="OQM87_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCS1507657.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17147..17398
                     /gene="mxiH"
                     /locus_tag="OQM87_001476"
     CDS             17147..17398
                     /gene="mxiH"
                     /locus_tag="OQM87_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCS1507658.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17411..17704
                     /gene="mxiI"
                     /locus_tag="OQM87_001477"
     CDS             17411..17704
                     /gene="mxiI"
                     /locus_tag="OQM87_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCS1507659.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17710..18435
                     /gene="mxiJ"
                     /locus_tag="OQM87_001478"
     CDS             17710..18435
                     /gene="mxiJ"
                     /locus_tag="OQM87_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCS1507660.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18432..18959
                     /locus_tag="OQM87_001479"
     CDS             18432..18959
                     /locus_tag="OQM87_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCS1507661.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18901..19596
                     /gene="mxiN"
                     /locus_tag="OQM87_001480"
     CDS             18901..19596
                     /gene="mxiN"
                     /locus_tag="OQM87_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCS1507662.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKR
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19589..19996
                     /gene="mxiM"
                     /locus_tag="OQM87_001481"
     CDS             19589..19996
                     /gene="mxiM"
                     /locus_tag="OQM87_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCS1507663.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19980..20408
                     /gene="mxiM"
                     /locus_tag="OQM87_001482"
     CDS             19980..20408
                     /gene="mxiM"
                     /locus_tag="OQM87_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCS1507664.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20538..21170
                     /gene="mxiE"
                     /locus_tag="OQM87_001483"
     CDS             20538..21170
                     /gene="mxiE"
                     /locus_tag="OQM87_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCS1507665.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21157..22857
                     /gene="mxiD"
                     /locus_tag="OQM87_001484"
     CDS             21157..22857
                     /gene="mxiD"
                     /locus_tag="OQM87_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCS1507666.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSRLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22876..23943
                     /gene="mxiC"
                     /locus_tag="OQM87_001485"
     CDS             22876..23943
                     /gene="mxiC"
                     /locus_tag="OQM87_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCS1507667.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23956..26016
                     /gene="mxiA"
                     /locus_tag="OQM87_001486"
                     /gene_synonym="virH"
     CDS             23956..26016
                     /gene="mxiA"
                     /locus_tag="OQM87_001486"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCS1507668.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26029..26430
                     /gene="spa15"
                     /locus_tag="OQM87_001487"
                     /gene_synonym="spaK"
     CDS             26029..26430
                     /gene="spa15"
                     /locus_tag="OQM87_001487"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCS1507669.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26434..27726
                     /gene="spaL"
                     /locus_tag="OQM87_001488"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26434..27726
                     /gene="spaL"
                     /locus_tag="OQM87_001488"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCS1507670.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27819..28175
                     /gene="spa13"
                     /locus_tag="OQM87_001489"
                     /gene_synonym="spaM"
     CDS             27819..28175
                     /gene="spa13"
                     /locus_tag="OQM87_001489"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCS1507671.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIRQYKPKF"
     gene            28144..29022
                     /gene="spa32"
                     /locus_tag="OQM87_001490"
     CDS             28144..29022
                     /gene="spa32"
                     /locus_tag="OQM87_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCS1507672.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIAANYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29016..29897
                     /gene="spa33"
                     /locus_tag="OQM87_001491"
                     /gene_synonym="spaO"
     CDS             29016..29897
                     /gene="spa33"
                     /locus_tag="OQM87_001491"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCS1507673.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29902..30552
                     /gene="spa24"
                     /locus_tag="OQM87_001492"
                     /gene_synonym="spaP"
     CDS             29902..30552
                     /gene="spa24"
                     /locus_tag="OQM87_001492"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCS1507674.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30567..30827
                     /gene="spaQ"
                     /locus_tag="OQM87_001493"
     CDS             30567..30827
                     /gene="spaQ"
                     /locus_tag="OQM87_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCS1507675.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30827..31597
                     /gene="spa29"
                     /locus_tag="OQM87_001494"
                     /gene_synonym="spaR"
     CDS             30827..31597
                     /gene="spa29"
                     /locus_tag="OQM87_001494"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCS1507676.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31604..32632
                     /gene="spa40"
                     /locus_tag="OQM87_001495"
                     /gene_synonym="spaS"
     CDS             31604..32632
                     /gene="spa40"
                     /locus_tag="OQM87_001495"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCS1507677.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSVFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32645..32887
                     /locus_tag="OQM87_001496"
     CDS             32645..32887
                     /locus_tag="OQM87_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507678.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            33397..33552
                     /locus_tag="OQM87_001497"
                     /pseudo
     CDS             33397..33552
                     /locus_tag="OQM87_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT        10496 a         4888 c         6724 g        11486 t
ORIGIN      
        1 tgacacttgt tgactacctg tgcggcatct ggtatcaagg tggcgtgaag ccggttcagg
       61 ttctcatgcg gaacgttgga acctatatcc ccccgatacc aaaggcgaaa tgtaagtgac
      121 aaaatgcaat gaacacgttc tccgttaact gaggagcatg gtgataaata aaggtaaatg
      181 cagaaggtgt agagtctcgc agatttctga catcatagag cctaacatta gatgcataac
      241 gctacagatt gtagaatctc tacaggtgaa atatacactg gttggcgttt atgtcgcaaa
      301 acctgtcatt caggttcatc tcaccaataa ggatatgagt gaagtggagg ataagtgagt
      361 ctgctgtcag agttttctgg tgtatgtcag taactgcgaa ccatgtctgc ttgatatggg
      421 agcttatgga ggctctctgt acaaggttag ggagctaaca aagcagggat aaaaagtccg
      481 cctgatgcag gcacggttcg ttaaatcttt tgccattggt aacaaaatgg tgcgatgaat
      541 gttcaggcta tctttatctt gatggtggtt cagctgtttg gtaaagaaat cgctgtaaca
      601 atagaagaac tgcaggcagt actggtccca catcgtttgc tcagtcaact ggtgaagttc
      661 cgtagcgcgt aaattaatgc tttgcatgaa acgttactgg agtggatgaa agaatacctg
      721 catatatcat tactgcgttg gattaccagt acaacagatt gaatgaactg gacatgttga
      781 tagacggcat tgaaaagcag cttataagca tagtgcggca gagttaattg gagagcactt
      841 cgccttctga gtattaagta agcatggtgg tacctgcgga tactatctat tcactgtgct
      901 cggtcggcag ccttactgac caaggagttg ggggagttga taacaaagtt gaaaaaacag
      961 ctctcaacca aggtggttat tgtaacaata gcaaataagc ttgtgtgacc tgtatcacaa
     1021 taaccgatta tggacgtaaa tacgacaaaa atatgtcagt atcaggcggt attaattgct
     1081 gatgctgatg gacattaact cttaataaaa gggtggatgc tgaaaggttg ttatggtgac
     1141 ggacgtgatg acgacggaaa agagtatgag gttttggatt taaagttcgt tcggggaata
     1201 aggacccggt cagctaattc tcatagtagt tcgcattgct ggatgcaata ctgtgctgca
     1261 attactcgtc gatgtcggaa caacggaagc cttgcaaacg gggatgtatt catacaggtg
     1321 ttatgttata tggggtttta ttgcaggggg ctataccaag agaatatatg atatctcatc
     1381 agactgatgt gagggtaaat gagaatagag taaacgaaca aggttgtttt ttggcgagga
     1441 agcagatgta tgacaattct tgtggagctg ccagtttact ctgtgctgca aaggaattag
     1501 gggttgataa aataccacaa tacaaagggt ctatgtccga aatgacaagg aagagttctc
     1561 ttgatttgga taatcgttgt gagcgggatt tatacttaat aactagtggt aattataatc
     1621 cgagaataca caaagataat atcgcggatg ccggttattc tatgcctgat aagatagtca
     1681 tggctacgag gcttcttgga ttgaatgctt atgtcgttga agaaagtaac attttttcgc
     1741 aagtaattag ttttatttat cccgatgcaa gggacttatt aattggcatg ggatgtaata
     1801 tagtacatca acgagatgtg ttatcttcta accaaagagt tttggaagct gttgctgtat
     1861 cctttattgg agttcctgtc ggcctccact gggtgttgtg tagacctgat ggaagctata
     1921 tggatccagc tgtaggagaa aattattctt gtttttcaac tatggaatta ggtgcaagaa
     1981 gaagtaattc taactttata ggatatacta aaataggtat ttccatagtt ataactaatg
     2041 aggctttgta aattgtcgtt ttttatctgt gaaggagctt gaatattttg gggcgggggg
     2101 ggcataacaa atgatattac tgagtacaaa tttagctata ttgggcggat tagttgtgtg
     2161 ggaagacgaa atgtttctct tgaaataagt gaactccctc aaggaatgat tgtgtgctta
     2221 aatatttaag tgctggtaag tttgagaagg ctgacatatt ttgcagagca aacagtgatt
     2281 gacttggatg atatttttat tttagtcact cgttgtaatt ggagtatgct ttattttatt
     2341 tgaaaataga tgtattttat acccgcaatt taaactgtaa gccctatgag gggtatggat
     2401 attataagtt actctttgac taagcataac ttttggggtt agaaaaataa atctcttgtt
     2461 aataattaat atgattttaa aatcaaactt gtttataatt ataaaataat gtaagtcggt
     2521 cattttatgg gaagattagg agaataaaac agtaattata tttgagaaat ctgctaatat
     2581 attcttaaag cttttttgat actgtgaacg aagtctgtta atggtggatt ttttgtttat
     2641 caccatgtaa aaaaattgag tagtaaaagc gttgactgct aatcctcgct gtttttattg
     2701 aaacgattaa aaatacgctg aatattttcg gccagtcact catcttcaca taaaattttg
     2761 accatcatgc cgcatccctt agttgcacaa ttcattttca atgatatgaa tattactaat
     2821 ttcaccagat aattttctcg tggtcagcag ggtatgagtt ttcaccgtaa ttttaaggaa
     2881 acacagtgcg aggggattga ataacagata gccgacggaa ttaccccacc ggctgaaagt
     2941 caagttcaac gaagattttt attatctgaa ttgggcagtt tacatcagtg ttcgatgttt
     3001 atgaagacga tagatggcga gaaattatat cccgaatagc ttcatctatt tctctctgaa
     3061 ctgttttgtt tattttagat aatgtaaata ttagagttct gtttgtttta ttctcttttc
     3121 tttttatgta agtccgtttg tctttagaaa taaataacga gtcaactttt agcgaagggt
     3181 tttgctttct gtttttgctt ttctttatga tgtttaaaat ttctttttta tatatgtccg
     3241 ggggcaaatt tgtatcaagg tcttttattt cttcctttaa tattggtagt gtagaactaa
     3301 gagattcatt agccttttca aggcctttat aataattgaa taatatcttg tagtcattaa
     3361 agttaagttc tgacgcgatt ggaaatagag atattatttc ctgtggaacg cttgctgcct
     3421 gaaaggcacg agtgactttc gcgcgagaca gattctcttt tttggctatg tctttatagg
     3481 acatccctga tactttcaga aaattaagac caataccaag ttctcggatg ctatgctctt
     3541 ttgctgtttg tatatcgttt gctagttttc tggcatcaag agtagatata tattcttttg
     3601 aatatagaac ttcaagatct gctcctgcat atattgcaga tgctcttcta cgagtgccat
     3661 ccagaatttc aattctacca tcaatctccc ttcctattac agggaagaat tgttgtagct
     3721 ttatagtttt tatgatatcg gctaatgatt cttctgttag ggattcttga tcgcgtacat
     3781 tcgttttttg gttgacgaag gttaaatctt ggatcctttt atgaggaatc ttggctttga
     3841 taaaggaaac tttattacca gaatggagtg taaactcttt cttttggcct tcctttatac
     3901 ttaacaagtc gttgcacaaa tccaccatat cacaccctgt ttattcatat tgatttatat
     3961 tgacaaaagt taaatgcagt gtgagcaaga ttgtatgtaa ctgttgatga tagtcaacgg
     4021 tagtcttaaa aattactatt ttaatagctg gattgatgca atttatcttt attggataaa
     4081 agaataatat tcttgtggag ctctgatgtg agagctccat agatgaaaaa atgcttatca
     4141 attactatat tgattttgta ctatttttga ctacagaaat aagatctcca atagtaataa
     4201 tgtgttctaa tgcagattca tctatacgta gattgaactc aaaactaatc atatccagca
     4261 tttcaatcag agaaagagaa tcggcagaaa gatcttcaat aaatttagtt tcttcagata
     4321 gtttgctgta cgctatacca tagcaaaagg ccactattga taatattttt tcttttatca
     4381 tattttaatc cttattgata ttctttaata cttttgatag ggaagtggtt acatcttttg
     4441 ctgctttata tatattattg ttttcagctg taatatcatt gttatttttt aaatcagata
     4501 tatcatctgt aagtaattct gcagaggtat cgtcaatttg tgacaatacc ttgctgaggg
     4561 cgctagatac ttcttttgcc ttttcataga tcgcatgatt tttatcaatt gtatctgcgt
     4621 tggtttctgt tacgtagttt attttatttg catctgtaac ttttaatttg ttgtttattc
     4681 tgtgttctgt tgtggatttt gtaatgtcct ttgatttttc ctgtaactct cgttcttgtg
     4741 ttcctgattt agttccaatg agagatataa gattacttag ggagtttgtt actttttttg
     4801 aagcttcaaa tatcgtttcc cgtgttaaat atgtgtctcc atttcttgat aaaaaatcag
     4861 tctgaagtac ttttgaatca gtgtttgagt tgaatttatt tatttgcgaa gctactctat
     4921 gagaaagaga agcgctactt ttagttgtat ctgatgagtt atcattactg cttgtcactc
     4981 cttccgggtg gagaggggag gtggttgaag agttctgtat gttcagaggc ctgtgtcccc
     5041 gagaaagaga gttatttgca gatctttctt gactactcgt agttaacagg tctgaaccta
     5101 ttccatccga attatctgga gtatggttga aaaccttttc taaagctgaa ttatttgcaa
     5161 atgtctggac agtatctgat atagagtgtg gtagaacgga agttactttt tgaactagtg
     5221 ctctttgtgg atttagtaat gagtttccgt tacgggataa tagatcgttt gtctgagaag
     5281 taggagactg tgcatcattt tctgggttct cgtgatagct attgtcatat actctgttgt
     5341 caaaaactct attatcgtaa gttctattat cattatttat atgatagtga attacaggtt
     5401 gggatgtttt ctcattggct ccgggggttg gtccgccatc tggcatatct tcgggaggtg
     5461 tagggaggcc atcttggata ggatgcgaat cttttatatc ttctaaaata tcattaagga
     5521 cttttctcaa cagctcagga aacacggcta ctgtatttaa gtctcttgac agtttaccta
     5581 taccaaatcc tttactatca atagtatttt tacgttgatt gcgcaaacta tcaaaggcaa
     5641 gttttgcctg tgcgtcaatt aaatcatcta gtttgagaga tgaaacagtt tcattactta
     5701 actttaattc agcaagaata ttattcttga taaatgatga tacttcagta ttgaggagtt
     5761 tttgtcccaa tgataaagct tcaccagatg tgtgttgttg tgctaatagt gcaagtctgt
     5821 gctcaatgtt ttgtcgtaat tgatggagtt tcttattctt tgccgaccat ggtccaaagg
     5881 cagaatcttg ggataattta tcttttataa ttagttccga atactgtgcg acaatttttt
     5941 gaagtccctt ttcgttttca aaattaagac cattttgatg tactaaggag tacaaaacat
     6001 ttgaaaattc gagaatctcg cttttggcat acgcattcat tgagttggaa taaatgcttc
     6061 ttaacacatc ttctttccct gaggaagaaa ataaaaaatc aagacatttc tgattaaaag
     6121 acgttgcaaa gttttctttt tcagacactg atgctggtgt ttttagagaa gtttgcgaac
     6181 tatggatatc ggatattttg ctttttaact cgctgtattc tgttgatgat ggtgaagttg
     6241 ccttatataa gaatgttggc gcttgagtat tatttacatt atgcatggcg cacctcagaa
     6301 atggagaaaa agtttatctg tatctgtaca tgagcttatt gtactactca aaacctttac
     6361 taaattatca aaaatactat tggcattact gtatttttga actaaagttt gaagattatt
     6421 tttcattgtt tcatcttcgg cagagaatcc ggcattccat gcctgatatt ttgcattatc
     6481 tagcacaacc tcgccatttc cacctagatt atctaagctt tttaacatat tgtctattgg
     6541 ggtcatgttt atactgacaa catatccccc gtttttttga gataccttgc cgattgttcc
     6601 acctaattct gtaagccatt tatttgcttg ttcctgacta acagtattat ttgctggata
     6661 tagcggttta tctttatatt tttccttgag ttcttccaat gcctttttaa gcgagttgac
     6721 ttgtaatttc acggagtttc cgtcgttacc tccgggagag atccagccgg caagactgga
     6781 aagaacagcg ctaaaatctt gatacatttg agtatatgaa ctaacggcat gttcatatac
     6841 tttcagatac tgttcattaa tatcattgat ggagtttgca attttagccc acagttctct
     6901 atgagatatc atttgatctc catcaagctc ggcttctttc ggggctgaat gtaataattc
     6961 tcttgcgtct ttattaattg gatattcgtt cctggaaaga atatccaata gttgagcgga
     7021 tttatttaca tccatgctaa tctgagatga atgtaatgct atttcttcta gcgaagtttt
     7081 agtcaacgtt ttttgtgaaa gctctttctt taatgcctta tttgttgttc tgatattatg
     7141 aagggtgtcg ttaagcatag taagggaact tacaggatga gaactggtcg ttgtttttat
     7201 atcagaatta actgtttcgg ttgatgaacc gttggtattg tttggactga atgatgaggt
     7261 ggaaatacta ttagtcagag ttgttatatt catggttatt tccttacagg aagagccata
     7321 tattattatg gctcttggat atattaagct cgaatgttac cagcaatctg actggctgcc
     7381 gaattccttg attgggtgat gctgtcaatt atattcaata atttttgtat taattgattt
     7441 gtcgcttggg atgcttcttt agatacttgg gatgcttcct ctgcttgttt actgctggcc
     7501 tgactgatta gttgttcttc ttcttcaaga gcagatgcat aacgccctcc tgatgtggat
     7561 atattaccag caacggctga tgtttccatt gttgcacggc caatgttttg tttttgctgc
     7621 gcagaaaggg tattgagctc gtaagttctt ctctgggtgt caattttatc ctgcaaagaa
     7681 atatcgggag aactaagaga agttttatgt tcagttgaca gggatatatt atctggcgcc
     7741 agtttatttt tacctaaaaa ttttgtgcta gagttagttt gtggtgaggt gatatttgta
     7801 tctatttgtt tatttaaccc taatttagaa cctgcaagct ctttttcaag agattgagca
     7861 gtggcaaggt tctttcttaa ggctcctttt tggtcgctaa tccctgaatg cgttttttta
     7921 gcaccgatac ccgttatacc tacttgtgtg actgctccag taatgcttga tgatagggct
     7981 gccaggcctt gccgaacaat gttctctgca gctgattttg tagcatcgaa cgcaatcaat
     8041 gacaattgag agcccaattt attttctgca gtgcggagag cagaaagtag aacggctaca
     8101 ctaataatta aagaaacagc attagaagaa agactggaaa tatccagagt gttctctgga
     8161 gtaagggtgt gactcatctc tgaaagatct ttcatctttt tgtcagtaag gcgagcaatg
     8221 atttctgaat catgctgaac ctgctctgat aactttaaaa gttgatcatc atttaatgtg
     8281 gttgttaata cgggattttt accgacatta agtggaatat gcgctgcaat ctgctgataa
     8341 ttttgtgatt tttgtgtttc ggaagaactt tgagtttgtt ttgtggatat atctgtatat
     8401 aaaatctggg ttggttttgt gttttgaatt tccataacat tctccttatt tgtatcaagc
     8461 agtagtttgt tgcaaaattg cttttgcaac atcagttcta ttagcctgag agttggacat
     8521 tgaggctaat agatctgcaa ttacttcctg caattggccg aatttttcta ttgcttcact
     8581 gatatatttt gacagttgtt caacttgata tttcgataat gtcaggtctg ctagatttgt
     8641 cgacgcgctg ttctggaaaa cagcagaagc gacacttcct cccgcttgtg tggcagagtt
     8701 aacactttct cctaataaaa ctgcttggtt taaatgggtg gaaataattt gtttggatat
     8761 tacttcatca cccgctgcac caagaaattt atttaatctg gcaacagcat tagtgattaa
     8821 atcgtccagt tgagaagaaa aattcttgag aaactttggt ataaggtctg tgagggtttt
     8881 acctattatt ttgccaatat tctctgcaag ttttgctgct gcctgtttac caacagtggc
     8941 tacgagaacg actgctgcaa ctaggacaag agcgcctgcg attgccccca gaatagagcc
     9001 aatcattttg gcttttttcg agtcgacgcc caagccttcg agcatttttg taaatgcatc
     9061 tgaaaggagt ttgattaagg gttcaatgac tgctttcatg atcggattca gggcttgttc
     9121 catgaaggaa ttgccggtcg ctgcttgtac tatagcatcc gtaaccataa gagctaaacc
     9181 aacagctgcc agtgctagag aggctcctcc agaaaaagct gctgcaacaa cactaacgat
     9241 agttaaaagt gccccaagta ttttcccaac acaacccatt actctgttga gttcttctgc
     9301 tttacgtact tcagcagcat actcatcaga ttttctctcc atttcagttt ttcttgattc
     9361 ttggagagac tggaatagag ccagatcatt ttttaaagat tcttcattat tttttccaac
     9421 tagttgaata aaggttgcca tcaattgagt aacactggca agtccggtta atgatttctg
     9481 ctgggttgat agctgttcag cagatgctgt gtttgaaaat gcagaaaaag agtctatttc
     9541 tttttcgagt tgcattgatt tatctgtaag tttgctatga attgacaggg ttttctgctc
     9601 aatcaatgtc ctgtctttaa ctgctgcgtc ttttttgata gtgagttgta tttcttcccg
     9661 gcttaatttt ttcttttctg gtgactctgg gtcgagttcg gataatcttg tttgaatttg
     9721 gttaatttta ttttctaggt cttttatttt agaatctgcg ttttttagtt tattaatttg
     9781 tttttcatag tctctggtta gtccttcagt ttcagataga agagtgttaa ttttatcgga
     9841 gaattctagg tttttttgct gtcttgcctg ttgctgggac ttccaagcag taattttatt
     9901 tgttaatgca gttaaagatt tttcaccgag tatttgaata aggtttccaa ttaaaagcgt
     9961 tagttgggaa cttgcattta atgactttgg tgctttaagt tcagggatta atatatttga
    10021 agttgagtgt gcattagttt tattaatatt ttggttagca gtaagatcag caattgtaag
    10081 agaaaataat ttattagctg catcatttgc agcttggata gtattgtctc caagctcagt
    10141 ggaagccaat attttggcaa gaggaaaacc agtggttgtg gtgcttacat tatgcataat
    10201 aattactcct tgatatcctg aattgcgtcc aagtatgatt gtgcttttat ttttaatttt
    10261 tcatcattgc tgtgttgaat tacgagttcg aagcactctt tagcttttaa gggggctttc
    10321 aaccgaagct ggcattgtcc agtatggaat actggtgtat agtcattttt tcctaatgca
    10381 aaagcgacag cataaaggtc tgctgcttgt tggaactgtt cttttatctg ataaatagct
    10441 gcgagtccca taatgtagtc tacattgtaa aagtcgtata tacataaaaa tctgaagaaa
    10501 acttcagctt cctctattct tcctttgttg taaaagtcat aagcatatga ataaatgtca
    10561 tccatcatat catcaggaat tgcattaata tctttcagtg tagcgccaga gttaattgca
    10621 tcaattactg cagtagagat gctttcattt tcagtgatat ttaaagacat aaggtctcct
    10681 ttttgtctgc tttactttaa taagtataag atttaatata aaagatttaa tttgtattgc
    10741 tttgacggta tacagccttt agaacctctg cgcttatttc tatttgtaaa acttttgttg
    10801 attcattaaa tattttatcg tagacggaac tggatactag atttgtaaga tccttcattt
    10861 gtgtgttaaa caaatccatg tcactgctat agcgttttaa aatctttgct ctgaggccag
    10921 atgaggcaat ctcgtttagt gctgtatgac tgatagatgt ttgtgctgat ttttcatcaa
    10981 gttttatatc cgggaatttt ttttcaatag ctgcaaatat gttttttttg ttatctgagg
    11041 tcaaaacaac tccattgtgt atggccattt catctatact tctgtttatc attgaagaga
    11101 cataagaggt tcgctcttgg ctcatccaac aaaatagttg attccccgaa ttactatctt
    11161 ttattttgtc tatgacgctg tccctgattt tatttatatt caccgggcat ccaaaattct
    11221 tctgaggaaa gcgttgtttt aagttaaaaa cagataatat tttctttaca agcttattgc
    11281 gacttaacga atcaaatgat gggcgaggaa tcgcgaattc aacctgtgga agtattttgt
    11341 ttagaatttg catgataccc cctatatgtt agttcacttc tgaagtgatg tttgcaatgg
    11401 ttagtttaat acaaaataaa aattcataca aagattcaat gagttcatca ggtgaaagat
    11461 attttttgta gtgtttcttg gtcaaaacac attgatgagt atcagggaga agacagatag
    11521 aagaaccata atctctactc aaagagtagt taaggatgag tatgtgttct ggattaataa
    11581 tattttctgt aaaaagttca tgttcattaa gaagtccggt caaaaagatg tattcatcat
    11641 tatctgttat acaaacaggt atttgttctt cgagcaatat aaaggcttga tttttatcgt
    11701 taaaatcaag ctttgcacct gttatttcat aaagtttatc atatagtttg cgacacattt
    11761 ctatggcctt ctatatatta gaatgagagt tattcaataa aagcttattg atctgtaata
    11821 agtcgtgaat gtaaagtttg gttccaagat ctggcgattt aagagaattg taataatcaa
    11881 taatttttat tgatttttcg ataatttctt cttttttatc tgttgcttcg gtttttaatt
    11941 cgtttaatac acgagtcata ttgctgggtg ctgattttac taagtatgag tggcaagcgt
    12001 tgatgaccac atcaaagtct tctgaagata cattttcagt aggatgtttg taaagtgaaa
    12061 ttgataaagg cttgaatgtt tttggtgtgt ggataaggtt aattttattg aaacttttaa
    12121 gatagctgcg tcgtaattga gtcaatctgg aaagatcaaa atcagattca atatacttca
    12181 ttatttcgtt ataagactga ttgagacttt caatgcgttg cctaacttta tttgcatacg
    12241 cgtatacatc gttaggtgta gcaaccaatt taacgtcagg gagtggaaag tatatttcag
    12301 aatttcctgc tttaagaaca ttaagtgcca taccagcaca gttttcagat ttactgatta
    12361 atttataatt tgccaattgt ctgaaagcat ctgctttctt tttgcatata aattttgaca
    12421 tgtttgtttc atcaaggcca aaaaggttat ggctgatttt accatccttt gttttattat
    12481 cacctgataa tggaatgtat actttatcag cactgacccc ccaataacca taggggtctt
    12541 ttttttgatt agctctggga aaaaatattt ttttgttgtt attttgatct tgttttgaat
    12601 ttaataagtt tctaacctct tggcctgcat caagtctttc ggctgtttgg tctgaaatca
    12661 tatttagttt gtcttgctta taggtatcga cactgtaacc taagcgtttt tcaaaaaggt
    12721 agtttatttc tgtatctttt gtaacatccc cgccgggata ccagctgaga taggtatgag
    12781 ttggttcctg tagtaaattg ttttttatac tgatactagt atgccctaaa tgggttatgc
    12841 ctctatttcc ccagcaacga aaaactaaat gtctttcaag atttttggct gtaccgatgc
    12901 catgaaaacg ctctgcggta ttcaacatta atttgatgtc tctacctgtg aaagagtgtt
    12961 gaattgagtt tttagctgct tcataggcgg caaacgcact ttctgcagat ttttcgctag
    13021 ctaatgtatt gaggaaaagc aataaggatt tttgattctc tagtcgataa ttttttagtt
    13081 gttcatttaa tatctgtgaa tttatctttg cattaatagt gcttaatgtt ctgaaaaaga
    13141 gatcctttag gttgaatttc tgtgctatca atataggtcc atgctctgta tcttcaacgc
    13201 gtataggccc ttttgtattg cttgcgtcaa tgaaattgct aattttgagg ctcatacttt
    13261 attaactctc cattacttgg tgattgtggg gatgtaaaat gtatttggca tataatatat
    13321 tcaattaata aatttagaaa cttgagcctg ttaacataat caaattttct tttgctgtac
    13381 ataatatgta cctcgtgagc atatgtagtg ctcgtttcat catgaaatcc cacaagataa
    13441 agtgcctgat gtatcaggct cggagtgtta tagaaaaaga gagaaccctg ttgaataagt
    13501 atcttttttg ttgtaaaacg aggtacaaaa ctgcaatcaa tgccccattt atgactgagg
    13561 ataattaaat gcacataact aatttgggat tgcatcaggt ttcatttcaa agcggagatt
    13621 cctataaagg cgcagaagaa accggaaagc acaaaggtgt aagcgtgatt tcatatcaaa
    13681 gagttaaaaa tggagaaaga aataagggga tcgaagcgct taatcgatta tatttacaga
    13741 accaaacaag cttaacagga aaaagccttt tgtttgcgcg tgacagagct gaagtttttt
    13801 acgaggcaat aaagttggca ggtggtgata cgtcaaaaat taaagccatg atggaacgat
    13861 tagataccta taaacttggt gaagttaata aaagacatat taatgagctt aataaagtaa
    13921 taagtgaaga gataagagca cagctaggca ttaaaaataa aaaggaattg caaacgaaaa
    13981 taaaacaaat atttactgat tatttaaata ataagaattg gggaccggtc gacaagaata
    14041 ttagtcatca tgggaaaaat tatggttttc aattaacacc tgcctctcat atgaagatag
    14101 ggaataaaaa tatatttgtc aaagagtata atggaaaagg aatttgttgt gcttctacga
    14161 gagagagtga gcatatcgcg aatatgtggc tttcaaaagt ggtggatgac gaggggaagg
    14221 aaattttttc gggtatcaga catggagtta tatcagccta tggattgaaa aaaaactcat
    14281 ctgaaagagc tgttgctgct cgtaataaag ctgaggagtt agtaagcgca gcattatata
    14341 gcagaccaga gttattatca caggctttgt ctggtaaaac agtagattta aagattgttt
    14401 cgacttctct tctgacgccg accagtttaa ccgggggaga ggagagtatg ttaaaagatc
    14461 aggtgaacgc attaaaaggg ttaaactcta aaagggggaa gccaacgaaa ttattaattc
    14521 ggaatagtga tggtcttctg aaagaagtaa gcgttaatct aaaagtggtg acatttaact
    14581 ttggtgtaaa tgaattagcg ctaaaaatgg gcttaggctg gaggaatgtt gacaagctta
    14641 atgatgaatc aatatgttct ttgttggggg ataatttcct caaaaatggt gtgattggag
    14701 gttgggctgc agaagcaata gaaaaaaatc caccatgtaa gaatgatgtg atatatctgg
    14761 ctaaccagat aaaagagatt ataaacaaga agttacaaaa aaatgataat ggagagccgt
    14821 ataaattgcc acaaagaatg actctattgg cttatactat aggtgctgta ccttgctgga
    14881 attgtaagag tgggaaggac agaacaggta tgcaggatgc tgaaattaaa agagaaataa
    14941 taagaaaaca tgaaacaggt cagttttctc aattaaatag taaattatcc tcagaagaga
    15001 aaagattatt ttctactatt ctaatgaata gtggtaatat ggaaatccaa gagatgaata
    15061 ctggtgttcc cggaaataaa gtcatgaaaa aattgccgct atcttcgtta gagctatctt
    15121 attctgaaag aataggggac ccaaaaatat ggaatatggt gaaagggtat tcgtcatttg
    15181 tataagagga atatatggaa gatttagcag atgttatttg ccgagccttg ggtatccctt
    15241 taattgatat cgatgatcaa gcaatcatgc ttgatgatga tgtgctaata tatattgaaa
    15301 aagaaggaga ttcaataaat ttattatgcc ctttttgtgc attgcctgaa aatataaatg
    15361 atcttatata tgcgttgagc ctaaattact cagaaaagat atgcttagct actgatgatg
    15421 aaggcggaaa tctaattgcg cgattagatc ttaccgggat aaatgaattc gaggatgtat
    15481 atgttaatac agaatattat atttcgcgtg tgcgttggct gaaggatgaa tttgcgcgaa
    15541 gaatgaaggg gtattaatgt cccgtttcgt atttattttg ctgtgtttta tcccatattt
    15601 agggagagct gattgttggg ataaggctgg tgaaaggtac aatattccat caagtttgtt
    15661 aaaagcgatt gcggaaaaag aatccggatt taataaatct gcagtcaatg ttaataacaa
    15721 tggaagtaaa gattatggta taatgcaaat aaatgacttt cattctaaaa gacttagaga
    15781 aatgggatat tctgaggaaa tgctaattag tcatccatgt ctttctgtac attatgcagc
    15841 aaagttatta aatgagttta tgatgatgta tggaagaggg tgggaggctg ttggagccta
    15901 taatgcagga acgtctccga aaaagaaaaa agaaagatta aagtacgcag aggatatcta
    15961 tagacgctat ttgcggatag cagcagagag caagcagaat aatcgaagga tataagagga
    16021 tttatgtctg aggcaaagaa ctcaaatctt gcaccattca ggttattagt gaaactcaca
    16081 aacggtgtcg gggatgagtt tccgttatat tatgggaata acttaattgt tctcggacgg
    16141 acgatagaaa cacttgagtt tggaaacgac aatttcccag aaaatattat tcctgtaaca
    16201 gatagtaaat cggatggtat aatttatttg accataagca aagataatat ctgtcaattt
    16261 agtgatgaaa aaggtgagca aattgatatc aattcacaat ttaattcatt tgaatatgac
    16321 gggatttctt ttcaccttaa aaatatgagg gaggataaat cgagaggcca tatactaaac
    16381 ggaatgtata agaaccactc ggtgtttttt ttctttgctg ttattgttgt gttaattata
    16441 attttttccc tctctttaaa gaaagatgaa gtaaaagaaa ttgctgaaat tatagatgat
    16501 aagagatatg gaatagtcaa cactggacag tgcaattata ttcttgccga aacacaaaat
    16561 gatgcggtat gggcgtctgt agccttaaat aaaaccggat ttaccaaatg cagatatatt
    16621 ctggtgtcaa acaaagaaat aaatagaatt caacaatata tcaatcaacg attcccattt
    16681 atcaatttgt atgtgttgaa tttagtgagt gataaagcgg aattactggt gtttttaagt
    16741 aaagaaagaa atagttcaaa agataccgaa cttgataaat tgaaaaatgc tctgattgtt
    16801 gaatttccgt atatcaaaaa tattaaattc aactatctta gcgatcataa tgcaaggggg
    16861 gatgctaaag gtattttcac aaaggtaaat gtccagtata aagagatttg tgaaaataat
    16921 aaagttacat attctgtcag agaagaactg acagatgaaa agcttgagct cattaataga
    16981 ctgatttctg aacataaaaa aatatatgga gatcagtata ttgagttttc tgtacttttg
    17041 atagatgatg attttaaagg taaatcatat cttaacagca aagacagtta tgtaatgttg
    17101 aatgataaac actggttttt tttagataaa aataagtgag gataaaatga gtgttacagt
    17161 accgaatgat gattggacat tgagttcatt atctgaaact tttgatgatg gaactcaaac
    17221 attacaaggt gaactaacat tggcactaga taaattagct agaaatcctt cgaatccaca
    17281 gttgctggct gaataccaaa gtaaattatc tgaatataca ttatatagga acgcgcaatc
    17341 caatacagtg aaagtgatta aggatgttga tgctgcaatt attcaaaact tcagataata
    17401 gggagcattc atgaattaca tttatccagt caatcaggtt gatattatca aagccagtga
    17461 ttttcaatct caagagatat caagtctgga agacgtcgtg tcggctaaat atagtgatat
    17521 taagatggat acagatattc aagtatcaca aataatggag atggtaagca atccagaatc
    17581 attaaaccca gaatctttgg ccaagttaca gacgacgctc tcaaattatt caataggagt
    17641 atcattagct ggcacgttag caagaaaaac agtttcggct gttgaaactt tattaaagtc
    17701 ttaatttata tgattaggta taaaggtttt attttattct tgttgctgat gttgattgga
    17761 tgtgagcaac gtgaagagtt aatttctaat ttatctcaaa gacaggcaaa tgaaataata
    17821 tctgtgctag aacgccataa tattactgct agaaaagttg atggaggtaa acaggggatc
    17881 tcggtacaag tcgaaaaggg gacatttgca tcggcagttg atttgatgcg catgtacgat
    17941 ttgccaaatc cggagagagt tgatatctca caaatgtttc ctacagattc attagtgtct
    18001 tctccaagag ctgaaaaggc ccgtttatat agtgctattg agcaacggct ggaacagtct
    18061 ttagtttcta ttggtggtgt tatttcggca aaaatacatg ttagctatga tcttgaagaa
    18121 aaaaatatat cttcaaaacc gatgcatata tcagtaatcg ctatatatga ctcaccgaaa
    18181 gagtctgaac tattagttag taatattaag cgatttttga aaaacacctt ttctgatgtt
    18241 aagtatgaaa atatatctgt catattaact ccgaaagaag aatatgttta tacaaatgta
    18301 caacctgtta aggaagttaa atcggaattt ttaacaaatg aagtaatata tttatttctc
    18361 gggatggctg tactagttgt cattcttttg gtatgggcat tcaaaacagg gtggttcaag
    18421 agaaacaaaa tatgataaga atggatggaa tttataaaaa atatctttca ataatttttg
    18481 atccagcgtt ctatataaat agaaatcggt tgaatttgcc ttctgaactg ttagaaaatg
    18541 gcgtaatcag aagtgagatt aataatctca taattaataa atatgatcta aattgcgata
    18601 ttgaaccttt aagcggggta accgctatgt ttgttgccaa ctggaattta cttccagctg
    18661 ttgcttattt tatcggtagt caagagtctc ggttaattaa tcatagtgag atggttatta
    18721 gttattatgg gggcaaaatc agtaagcaag gggaggccgc tattcgaagt ggtttttggc
    18781 atcttattgc gtggaaggaa aatatatctg ttggtatata tgaaagaata aacttattat
    18841 ttaatccaat tgctttggag ggaaattata cgcctgtaga aagaaatttg tcccgattaa
    18901 atgaaggtat gcaatatgca aaaaggcact ttaccggtat ccagacatca tgcctatgat
    18961 ggtgttgtta taaagagaat tgaaaaggaa ttatgtaaaa ccataaaaga tagagacact
    19021 gagagtaaga ggaaagcaat ttgtgttata aaggaggcta cgaaaaaagc tgaatcactg
    19081 cgtatagatg ctgtatgtga tggttatcaa attggtatac agactgcttt tgagcatatt
    19141 attgactata tttgtgaatg gaagttaaag caaaatgaaa acagaagaaa tattgaggac
    19201 tatataacta gtttgctttc agaaaattta catgatgaga ggatcattag cactttattg
    19261 gagcagtggc ttagttcgtt gcgtaataca gtaacagaat taaaagtggt tttgcccaag
    19321 tgtaatttag cactgagtaa aaagttagaa cttgatctcc ataaatatcg ctcagatgtc
    19381 aagattattt taaaatattc tgaagggaat aattatatat tctgtagtgg taatcaagta
    19441 gtggagttta gtcctcagga tgtaatatca ggtgttaaaa tagaacttgc ggaaaaatta
    19501 acaaaaaatg ataaaaaata tttcaaagag ttagcacata agaaactgag acaaattgca
    19561 gaagacttgt taaaagagaa tcctgttaat gattaatcaa ataaatgcaa gcaatgctct
    19621 gcagcaaaga ttaaatagtg aagaatttgt taatttaaat gagagattat catcttcgca
    19681 atcatttgat gaggatataa tctatgaaat aatgcaatat ttctcacaga gtgaattaaa
    19741 ttccatagat aatgatgaac tgcacaataa gattgaacaa ttattcaatt cacgttttcc
    19801 ttatcttaca gctgcacaaa aaagttcact tcttaataaa ctcatagatg caaatcaata
    19861 tgtcgatttg catgagggat tttatgcatc attgtccata tataacaata ttgatttcta
    19921 tattaagact acaaccttcg attcattaat tagtgtcttt gaagcaggga gagaggcaga
    19981 tgattcgaca tggtagtaat aagttgaaaa tatttatttt aagtatattg ctattaacac
    20041 tgagtgggtg tgctttaaag tcatcatcta attctgaaaa agaatggcat attgttcctg
    20101 taagtaagga ttatttttct attccaaatg atttattatg gtcgtttaat acaaccaata
    20161 aaagtataaa tgtttactct aaatgtatta gtggtaaggc ggtttatagt tttaatgcag
    20221 gtaaattcat gggcaacttt aatgttaagg aagtagatgg gtgcttcatg gatgcacaaa
    20281 agatagctat agataaacta ttttctatgc tgaaagacgg ggttgtttta aaaggtaata
    20341 agataaatga taccatcctt atagagaagg atggggaagt taaattaaaa ttaattcgag
    20401 ggatataatt gtattgtgag taaatataaa ggtctaaata caagtaatat gttttacatt
    20461 tactctagtg gacatgaacc agttaacgtt gagcttgtaa aagataaaga acgtaacata
    20521 attgagctgg ctccagcatg gaagggtttt tttttgtccg aaatcaaaat ataaaattca
    20581 gtgataatgt gaactatcat tatcgcttta acattaatag ttgtgcaaaa tttcttgctt
    20641 tttgggacta tttcagtggt gctctagtag agcatagcca tgctgaaaaa tgtattcatt
    20701 tctatcatga gaatgatctt cgggatagtt gtaataccga atcgatgctt gataagttaa
    20761 tgttaagatt catcttttca tctgaccaga atgtaagtaa tgcactggct atgatacgaa
    20821 tgactgagtc atatcatttg gttctttatc ttctgagaac tattgagaaa gaaaaggagg
    20881 taagaataaa gagtctgaca gagcattatg gtgtgtcgga ggcttatttt agatctttat
    20941 gtagaaaagc tttaggggca aaagtcaaag aacaactgaa cacttggcgg ttagtaaatg
    21001 gtttattaga tgtatttttg cacaatcaaa caatcacttc tgctgccatg aataatgggt
    21061 atgcatcaac atcccatttt tcaaatgaga taaaaacacg actaggcttt tctgcaagag
    21121 aattatcgaa tattactttt ttagtgaaaa aaataaatga aaaaatttaa tattaaatct
    21181 ttgactctct tgattgtatt gttaccattg atagttaatg ctaataatat tgactctcgt
    21241 ttattagaac aaaatgacat agcgaaatat gttgctcaaa gtgatacggt aggatctttt
    21301 tttgaacgtt tttctgcgtt attaaattat cctattgttg ttagcaaaca agctgcaaaa
    21361 aagagaatat ctggggagtt tgatttatct aatccagaag aaatgcttga aaaactgaca
    21421 ttacttgtgg gactcatatg gtataaagat ggaaatgcat tatacatata tgactctgga
    21481 gaattaataa gcaaagttat tttgctggag aatatatcac ttaattatct tattcagtat
    21541 ctgaaggatg ctaaccttta tgatcacaga tatcctataa gaggaaacat tagtgataaa
    21601 acattctata tatctggtcc tccggcattg gttgaactgg tagctaatac agcaactttg
    21661 cttgataagc aggtttcttc tattgggaca gataaagtta attttggggt tataaaatta
    21721 aaaaatacat ttgttagtga cagaacttat aatatgagag gggaggatat tgtcatccca
    21781 ggggtggcta ctgttgtaga acgtttatta aataatggta aggctctgag taacagacaa
    21841 gcccaaaatg atccgatgcc accctttaat attacacaga aagtatctga ggatagtaat
    21901 gacttttcat ttagttcagt taccaatagt tctatattag aggatgtatc acttattgca
    21961 tatccagaaa ccaattctat attagtcaaa ggtaatgatc agcaaataca gattattcgc
    22021 gatattatca ctcagttaga tgtagccaaa cgacatatag aattatcatt atggataatc
    22081 gatattgata aatctgaatt aaataacctt ggtgttaatt ggcaaggaac ggcttcattt
    22141 ggtgattcgt ttggtgcgtc atttaatatg agctcatcag catcgataag cactttggac
    22201 ggcaataagt ttattgcttc agttatggca ttaaatcaga aaaagaaagc aaatgtagtt
    22261 tcaaggccgg tgattttgac acaagagaat attccggcta tatttgataa taacaggact
    22321 ttttatgtgt cattagttgg tgagcgcaat tcttcgttgg agcatgttac gtatggaaca
    22381 ttgataaatg ttatacctcg cttttcatct cggggacaaa ttgagatgtc tttaacgata
    22441 gaggatggaa caggaaatag tcagtcaaat tacaattata ataatgaaaa tacatctgtc
    22501 cttccagaag ttggaaggac caaaatcagt acaatagcga gagttccaca aggcaaaagt
    22561 ttgttgatag gtggttatac gcatgaaacg aactctaacg aaatagtatc tattcctttt
    22621 ctctcctcta ttcctgtaat cggtaatgtg ttcaagtata aaacaagtaa tatcagtaat
    22681 attgtaaggg tttttctgat tcaaccccgt gagataaagg agtcaagtta ttataatact
    22741 gcagaatata aaagcctcat atctgaaaga gaaatacaaa agactacaca aataatccca
    22801 tctgaaacta ctttgctgga agacgaaaaa tcattggttt catacttaaa ttactaacta
    22861 taaagtaggt gatgtatgct tgatgttaaa aatacaggag tttttagctc tgcattcatt
    22921 gataagttga atgcaatgac aaattcagat gatggagatg agactgctga tgcagagctt
    22981 gattctggct tggctaatag caagtatatt gactcatctg atgagatggc ttccgctctt
    23041 tcgtcattta taaacagaag agaccttgag aaactgaaag gaacaaatag tgatagtcag
    23101 gaacgtattt tagatgggga agaagatgaa attaatcaca agatttttga tttaaagaga
    23161 acgttaaaag ataaccttcc tctagatcgg gattttatag acagactaaa gagatatttt
    23221 aaagatccaa gtgatcaagt cttagcatta agggaacttt tgaatgaaaa agatcttact
    23281 gctgaacaag tcgaattatt aactaaaatt attaatgaga taatatcagg tagtgaaaaa
    23341 agtgttaatg ctggaataaa ttcagctata caggctaaat tatttggcaa caaaatgaaa
    23401 cttgaaccac agcttttgcg tgcatgttat cgtggtttta tcatggggaa catatcaaca
    23461 acagatcagt atatagaatg gcttggtaat tttggtttta atcacagaca tacaattgtg
    23521 aattttgtag agcagtcact gattgtagac atggattctg agaaaccgag ctgtaatgct
    23581 tatgagtttg gttttgtgtt atctaaatta attgcaatta agatgattag aacttcagac
    23641 gtaattttta tgaagaaact ggaatcctca agcttgctaa aagatggcag tttaagtgca
    23701 gagcagctat tgctaacttt attatatatt tttcaatatc caagtgaaag tgagcaaatt
    23761 cttacttctg ttatagaagt atcacaagcc agtcatgagg attctgtagt gtatcaaaca
    23821 tatctatctt ctgttaatga aagtcctcat gatatattta aaagtgaaag tgaaagagaa
    23881 attgcgatca atattctacg agagcttgtc acaagtgcat acaagaaaga gctttctaga
    23941 taacaggaga taaaagtgat ccagtctttt cttaagcaag taagtactaa gcctgaatta
    24001 attatattag tgcttatggt tatgatcatt gctatgttga tcataccatt acctacatac
    24061 cttgttgatt ttttaatagg gcttaatatt gtacttgcta ttctcgtttt tatgggatca
    24121 ttttatattg aaagaatatt gagtttttct acattccctt cggttttgtt gataacgaca
    24181 ttatttcgtc tcgctttatc aataagtact agtcgactaa ttttagttga tgcggatgcg
    24241 ggtaaaatta ttactacatt tggccagttt gtcattggtg atagtctggc tgtaggtttt
    24301 gttatttttt caatagtaac tgttgtgcag ttcattgtta taacaaaagg atctgaaagg
    24361 gttgcggagg tagctgctcg cttttctctt gacggtatgc cggggaaaca aatgagcatt
    24421 gatgcggatt taaaggctgg aattattgat gccgcaggag ctaaagaaag acgcagtatt
    24481 ctggaacgtg agagtcaatt gtatggttca tttgatggag caatgaagtt cattaaaggt
    24541 gatgcaattg ctggcatcat tattattttt gtcaatttaa taggaggtat ttctgttggt
    24601 atgagtcaac atggaatgtc cctatctggt gcattatcta cttataccat actaactatt
    24661 ggtgacggat tggtctctca gattcctgct ctgttaattt ctataagtgc aggatttatc
    24721 gttacaagag ttaatggtga tagcgataat atgggacgta acataatgtc ccagatattt
    24781 gggaatcctt ttgttctcat tgttacatca gctcttgcct tggcgatagg gatgttgcca
    24841 ggttttccgt tttttgtttt tttcctgata gcagttactt tgacggcttt attctattat
    24901 aaaaaggtcg tagaaaaaga aaaaagtctg tctgagtctg attctagtgg ctatactggt
    24961 acatttgata ttgataatag ccatgactca tctttggcaa tgatagaaaa tctggatgct
    25021 attagttcag aaaccgttcc tttaatttta ttatttgccg aaaataagat aaatgcaaat
    25081 gatatggaag gtcttattga aaggataaga agtcagttct ttattgatta tggtgtcagg
    25141 cttccaacta ttttatatag aacaagcaat gagcttaagg tcgatgatat tgttttgtta
    25201 ataaatgagg tgcgtgccga tagttttaat atatattttg acaaagtgtg tattacagat
    25261 gaaaatggag atatagatgc tctaggtatt cctgttgttt caacttcata taatgaacgt
    25321 gttatttctt gggtagacgt ttcatataca gaaaatctaa ctaatattga tgctaaaatt
    25381 aaaagtgctc aagatgaatt ttatcaccag ttgtcacaag ctttattaaa caacataaat
    25441 gagatttttg gtatacaaga aacaaaaaat atgttagatc agtttgaaaa tcggtatcct
    25501 gatctattaa aggaagtctt ccgacatgtg actatacaga gaatttctga ggtattacaa
    25561 agattgcttg gagaaaatat ttctgttcgc aatttaaaac ttattatgga gtctttggcg
    25621 ctttgggctc ccagagaaaa agatgtcata acattagttg aacatgtccg tgcatcactt
    25681 tctaggtata tttgtagtaa aatagctgtt tctggtgaga ttaaagttgt gatgctttcc
    25741 ggatatattg aggatgcaat aagaaaaggg ataaggcaaa cctctggtgg ctctttcttg
    25801 aatatggata tagaggtttc ggatgaggta atggaaactt tagcacatgc tttgagagaa
    25861 ttgagaaatg caaaaaaaaa tttcgttctt ttggtatcag tagatatacg taggtttgtt
    25921 aaaagactta tagataacag atttaagagt atactcgtta tatcgtatgc tgagattgat
    25981 gaagcatata ccattaatgt attaaagact atttagtgag gtttaaatat gagtaacatt
    26041 aatttagttc aattagttag agatagtctt ttcacgattg gttgtcctcc atcaattatt
    26101 acagatttag acagccattc agcgattacc atttctttgg atagtatgcc tgctattaat
    26161 atagctcttg tcaatgagca ggttatgtta tgggctaatt ttgatgcacc aagtgatgta
    26221 aaacttcagt catctgctta taacatatta aatttaatgt taatgaactt tagttatagc
    26281 attaatgagc tggtagagct tcatcgttct gatgagtact tacagttaag agttgttatt
    26341 aaagatgact acgttcacga tgggattgtt tttgcagaga ttctgcatga gttttatcag
    26401 aggatggaaa tcttaaatga ggtcttataa tcaatgagct atacaaaatt gctcactcaa
    26461 ttatcttttc ctaatagaat ctcggggcca atcttggaaa caagtcttag cgatgtttcg
    26521 attggtgaga tttgtaacat tcaggctgga attgaaagta atgaaattgt tgcaagagct
    26581 caggttgtag gatttcatga tgaaaaaaca atattaagct tgattggaaa ttctcgtgga
    26641 ctttcacggc aaacgttgat taagcccact gcccagtttc ttcatacgca agtcggccgt
    26701 ggattattgg gagcagtagt caatccttta ggggaggtta ctgataagtt tgctgttacg
    26761 gataacagtg aaattcttta tcgacctgta gataatgctc ctccgctata tagtgaaagg
    26821 gctgcaattg agaagccttt tttaacaggt attaaggtta ttgattcttt actcacgtgt
    26881 ggtgaaggac agcgaatggg gatttttgcg tcagctggtt gtggcaaaac ttttctcatg
    26941 aatatgctca ttgaacatag tggtgctgat atatatgtta ttgggttaat tggtgagcga
    27001 ggtcgagagg ttacagaaac ggttgattat ttgaaaaact ctgagaaaaa aagcaggtgt
    27061 gttttagtat atgcaacttc ggattactct tcggttgatc gttgtaatgc tgcatatata
    27121 gccactgcta tagccgaatt ttttaggact gaaggacata aagtagcgct ttttattgat
    27181 tcattaacaa ggtatgccag agcattacgt gatgtggcct tagccgctgg agaatcacct
    27241 gccagaagag gctatccggt ttcggttttt gatagcttac ccagacttct tgaaaggcca
    27301 ggaaagttaa aggcaggtgg ctctattact gcattttaca ctgttctttt ggaggatgat
    27361 gattttgctg atccattagc tgaagaggta agatccattt tagatggaca tatatatttg
    27421 agcagaaatc tagcccaaaa aggacaattt cctgcaattg attccttaaa aagtataagc
    27481 agggtattta cacaggttgt tgatgaaaaa catcgtatta tggccgctgc atttcgggag
    27541 ctactttctg aaatagaaga gctaaggaca attattgact ttggtgaata caaaccgggg
    27601 gagaatgcct ctcaggataa aatctacaac aaaatatctg ttgttgagag ttttctgaag
    27661 caagattatc gactcggttt tacttatgag cagacaatgg agcttattgg tgaaacaatt
    27721 agataaggtt ttaaaaatca aagataaata tcaacggtct gttaaattaa ttgaagcaca
    27781 catattaaca ctaaaaaaaa aactctttgt atcgagatgt ggaggcatta gataaaagga
    27841 ttatatactt cctgcaactg gagaatgact tagaacctgt aggcgctcag agcgttagtc
    27901 agcttttcaa tacaagacgt aagatcgcta tagtaaaaaa gcatattatc caatatcagt
    27961 ctgaacgaat tcttctaaag ggaagaattg aagaaattca aaaagatatt gatgaggcaa
    28021 atgcttctaa aagaaaactg cttcataaag agagtaaaat atgtaaacgg attggcttga
    28081 ttaaaagaaa taactttgcc aaacagttga tacttgatga gttatcacaa gaggatatga
    28141 agtatggcat tagacaatat aaacctaaat tttagtagtg acaaacagat agaaaaatgt
    28201 gagaaactat cttcaataga taatattgat agtctcgttt tgaagaagaa gaggaaggtt
    28261 gaaattcctg agtactcttt aattgcagct aactatttta ctattgataa gcactttgaa
    28321 cataagcatg ataaaggaga aatttatagt ggcattaaaa atgcgttcga acttagaaac
    28381 gaacgagcga catattctga tattccagaa tcaatggcca ttaaagaaaa tattttgata
    28441 ccagatcaag atatcaaagc aagggaaaaa ataaatatcg gcgatatgag ggggatcttt
    28501 tcatataata agagtggaaa tgcagacaag aacttcgaaa gaagtcatac ttcttctgta
    28561 aaccctgata atctgctaga atctgataat agaaatggtc aaattggttt aaaaaatcat
    28621 agcttgtcta ttgataagaa tattgctgac atcatttctt tactaaatgg aagtgttgct
    28681 aaatcatttg agctccctgt aatgaataaa aatactgcag acataacccc atccatgtca
    28741 ttgcaagaaa aatcaatagt tgaaaatgat aaaaatgttt ttcaaaaaaa tagtgaaatg
    28801 acttaccact ttaaacagtg gggggctgga cattctgtta gtatttcagt ggagtctggt
    28861 tcttttgttc taaaaccgtc agatcaattt gtaggaaata aacttgactt aattttgaaa
    28921 caagatgctg agggtaatta cagatttgat agcagtcaac ataataaggg gaataaaaat
    28981 aatagtacag gatataatga acagagtgaa gaagaatgct aagaattaaa cattttgacg
    29041 ctaacgaaaa actacagatt ttatatgcaa agcaactctg cgagcgtttt tcgattcaga
    29101 cattcaaaaa taaatttaca ggcagtgaaa gtttagtcac tcttacttcc gtgtgtgggg
    29161 attgggtaat tcgtattgat acattatctt ttttgaaaaa aaaatacgag gtattttcag
    29221 gattttctac acaagaatct ttactgcatt tatcaaaatg tgtctttata gagtcgtcat
    29281 ctgtattttc gattccagaa ctgtctgata agattacttt ccggatcacg aatgaaatcc
    29341 agtatgcaac tactgggagt catttatgct gtttttcctc ttctttaggt attatttatt
    29401 ttgacaagat gccggtatta cgtaatcaag tttctcttga cttattgcat catcttttag
    29461 agttttgctt aggttcatct aatgtaaggc tggctacttt aaaaagaatt cgcactggtg
    29521 atataatcat agttcagaaa ctttataatt tattattgtg taatcaagtt attattgggg
    29581 attatattgt gaatgataat aatgaggcaa aaattaatct gtcagaaagt aatggtgagt
    29641 cagaacacac agaagtttct ttggcattat tcaattatga tgatatcaat gtaaaagtgg
    29701 actttattct tttagaaaaa aatatgacaa tcaatgaact aaaaatgtat gtagaaaacg
    29761 aattattcaa gtttcccgat gacatagtta aacatgtaaa tattaaagta aatggttctt
    29821 tggttgggca tggggaactt gtttctattg aggatggtta tggtatcgag attagttctt
    29881 ggatggtaaa ggagtaatct catgctgagt gacatgtccc tcatagctac actttctttt
    29941 tttacgctat taccattttt agttgctgct ggtacctgtt atataaagtt ttctattgtt
    30001 tttgtaatgg tacgaaatgc acttggtttg cagcaggttc catcaaatat gactctcaat
    30061 ggtattgcat tgattatggc tttgttcgtg atgaaaccca ttattgaagc tggttatgaa
    30121 aattatctaa atggccctca aaaatttgat acaatatctg atattgtccg tttttctgat
    30181 tctggcttga tggaatataa gcaatacttg aaaaagcata cagatcttga gttagcacgt
    30241 tttttccaaa ggtctgaaga agaaaatgct gatttaaaga gtgcagagaa taatgattat
    30301 tcattgtttt ccctcttgcc tgcatatgcg ctaagtgaaa tcaaagatgc gttcaaaata
    30361 ggtttttatt tatatttgcc atttgttgtt gttgatcttg ttatttcaag cattttactt
    30421 gcacttggta tgatgatgat gagtccaatc acaatatcag taccaataaa acttgtatta
    30481 tttgttgcgc ttgatgggtg gggtatcctg tctaaggcct tgattgaaca atatatcaat
    30541 attcctgctt agatgatgga ggtaatgtgt ctgatatagt ttatatgggt aataaggctc
    30601 tttatttaat ccttatcttt tccttatggc cagtaggtat agctacggtt attggattat
    30661 ctattggttt gttacagaca gtgactcaac tccaagagca gacacttcct tttggtataa
    30721 agcttatagg tgtctcaata tctttgctac ttctttctgg atggtatggt gaggttttat
    30781 tgtctttttg tcatgaaata atgtttttaa ttaagagtgg ggtttgatgg acatttcaag
    30841 ctggttcgaa agtattcatg tgtttttaat actcctgaac ggcgtttttt ttagattggc
    30901 tccattattt ttctttcttc catttttaaa taacggtata atttctccat ctattagaat
    30961 acctgtgatt tttcttgttg catcaggatt aattacttct ggtaaggtag acataggttc
    31021 ttctgttttt gaacatgttt atttccttat gttcaaggaa ataattgttg gcctccttct
    31081 ctctttttgc ttgtctcttc ccttttggat atttcatgct gttggtagca ttattgacaa
    31141 ccagcgtggg gcaacgctta gtagttcaat tgatcctgcc aatggtgttg atacgtctga
    31201 gttggcaaaa tttttcaatc ttttttctgc agttgtattt ctatacagtg gtggtatggt
    31261 ctttatttta gaatccatac aattgtctta taatatatgc ccgttatttt ctcaatgttc
    31321 tttccgtgtc tcaaatatct taacatttct gactttattg gcaagtcagg ctgttatttt
    31381 agccagtcct gttatgatag tattgttact atcagaagta ttacttggtg tattatcgag
    31441 atttgctccg cagatgaatg ctttttccgt atcattaact attaaaagtt tacttgcaat
    31501 atttattatt ttcatctgtt cttctactat ttacttttct aaagttcaat ttttcctcgg
    31561 tgaacataaa tttttcacaa atctatttgt tagataaaat attatggcaa ataaaacaga
    31621 aaagccgaca cctaaaaaac taaaggatgc cgcaaaaaaa ggacagtcat ttaaatttaa
    31681 ggatttaacg actgttgtta ttattctggt agggacattt actataatat cattcttttc
    31741 cttaagtgat gtaatgctct tatacagata tgtaataatt aatgacttcg aaattaatga
    31801 gggtaaatac ttttttgcag tggttattgt cttttttaag ataattggct tcccactttt
    31861 tttctgtgtt ctttcggctg tgttgccaac attggttcaa acaaagtttg ttcttgcgac
    31921 taaagctatc aagattgatt tttcagtatt aaaccctgtt aaagggttaa aaaaaatatt
    31981 tagtataaag acaataaaag aatttttcaa aagcattctg cttcttatta ttctagcatt
    32041 aacaacctat ttcttttgga ttaatgaccg aaaaataatt ttttctcagg tgttttctag
    32101 tgttgatggc ttatatctta tttgggggag gctgtttaag gatataatat tatttttctt
    32161 ggcattttct gtttttgtta ttattcttga ctttgtgatt gagttcattt tatacatgaa
    32221 agatatgatg atggataaac aggagataaa aagagaatat atagagcaag agggacactt
    32281 tgagacaaag tcgagaaggc gtgagttgca tatcgagatt ctttcagagc agactaaatc
    32341 tgatatacgt aattcaaaat tagtggtaat gaacccgact catattgcaa ttggtattta
    32401 ttttaatcca gaaatagcgc ctgcaccttt tatttctctc attgaaacta accagtgtgc
    32461 cttggctgtc agaaaatatg caaatgaagt tggtataccg actgtgcgtg atgtgaaatt
    32521 agctagaaaa ctatataaaa cacatacaaa atatagtttt gttgattttg aacacttgga
    32581 tgaagtccta cgtcttattg tttggcttga gcaggttgaa aacactcatt aataagtaaa
    32641 ggagatgtgt tatatgggag ttaatttctg taataaaata ggtattgatc agtctgaatt
    32701 tgaaatagaa tcttccatca ttaactccat tgctaatgag gtattgaacc caatatcttt
    32761 ccttagcaat aaggatataa taaatgtttt actcagaaag atttcatctg aatgtgacct
    32821 ggttagaaaa gacatttatc gttgtgctct ggagttggtc gttgaaaaaa cacctgatga
    32881 tttataatca ttcataataa ctcttttgct gatataaata tttttgttga agatatttat
    32941 gtagtcacag gatcaattgt agaaaattgt tatgtttttg ttaggaactg cactaaataa
    33001 tatatgataa gacagcaaaa acgactgacc ataatccttc ttttattagg agttgataag
    33061 agagactatt catcctgtaa tgttaaaact ctgttatata gtattcgcga ttatgcaaag
    33121 tctgtaaatg atcatgagat actaaccgag agtaatcgtt tattatctca ttgcatttca
    33181 gatagtaacg gagctttctt taaaagtagt aaatacgttc cccttaagta cttacgtaaa
    33241 aggagaatag cacgaaagat accaaatgat tagcactttg ctagtaagct tgctggtaaa
    33301 ctatattgac cgttgggttt gttttgagac tgtaatttaa attgtgtaat tgcctgtttt
    33361 tgatatgttt actccaacaa cggagacagg taaattatgg acgaaaagaa gcttaaagcc
    33421 cttgcggctg aactggctaa aggcctcaaa accgaggccg acctcaatca gttttcccgt
    33481 atgcagacga aattaactgt cgaaacggtg cttaatgcgg aactaactga ccatctcggg
    33541 catgaaaaaa gctacatccg gtagtaaaca ttatcagtgg gttatgcgat atga
//
LOCUS       DAJVQH010000029        33129 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJVQH010000029 DAJVQH010000000
VERSION     DAJVQH010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.029"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..224
                     /locus_tag="OQM87_001498"
     CDS             <1..224
                     /locus_tag="OQM87_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCS1507679.1"
                     /translation="PQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVL
                     DGKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            398..1167
                     /locus_tag="OQM87_001499"
                     /pseudo
     CDS             398..1167
                     /locus_tag="OQM87_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1200..2720)
                     /locus_tag="OQM87_001500"
     CDS             complement(1200..2720)
                     /locus_tag="OQM87_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1507680.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNERAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            2811..3530
                     /gene="tonB"
                     /locus_tag="OQM87_001501"
     CDS             2811..3530
                     /gene="tonB"
                     /locus_tag="OQM87_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCS1507681.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(3570..3968)
                     /gene="yciA"
                     /locus_tag="OQM87_001502"
     CDS             complement(3570..3968)
                     /gene="yciA"
                     /locus_tag="OQM87_001502"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCS1507682.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4073..4612)
                     /locus_tag="OQM87_001503"
     CDS             complement(4073..4612)
                     /locus_tag="OQM87_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCS1507683.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(4642..5385)
                     /locus_tag="OQM87_001504"
     CDS             complement(4642..5385)
                     /locus_tag="OQM87_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCS1507684.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(5413..5502)
                     /locus_tag="OQM87_001505"
                     /pseudo
     CDS             complement(5413..5502)
                     /locus_tag="OQM87_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5742..6380
                     /gene="ompW"
                     /locus_tag="OQM87_001506"
     CDS             5742..6380
                     /gene="ompW"
                     /locus_tag="OQM87_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCS1507685.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(6440..6946)
                     /locus_tag="OQM87_001507"
     CDS             complement(6440..6946)
                     /locus_tag="OQM87_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCS1507686.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDCVEAKK"
     gene            complement(6992..7492)
                     /locus_tag="OQM87_001508"
     CDS             complement(6992..7492)
                     /locus_tag="OQM87_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCS1507687.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESKSNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(7578..7757)
                     /locus_tag="OQM87_001509"
     CDS             complement(7578..7757)
                     /locus_tag="OQM87_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCS1507688.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(8138..8944)
                     /gene="trpA"
                     /locus_tag="OQM87_001510"
     CDS             complement(8138..8944)
                     /gene="trpA"
                     /locus_tag="OQM87_001510"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCS1507689.1"
                     /translation="MERYESLFAQLKERKEGAFIPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(8944..10137)
                     /gene="trpB"
                     /locus_tag="OQM87_001511"
     CDS             complement(8944..10137)
                     /gene="trpB"
                     /locus_tag="OQM87_001511"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCS1507690.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(10149..11510)
                     /gene="trpCF"
                     /locus_tag="OQM87_001512"
     CDS             complement(10149..11510)
                     /gene="trpCF"
                     /locus_tag="OQM87_001512"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCS1507691.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(11511..13106)
                     /gene="trpD"
                     /locus_tag="OQM87_001513"
     CDS             complement(11511..13106)
                     /gene="trpD"
                     /locus_tag="OQM87_001513"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCS1507692.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(13106..14668)
                     /gene="trpE"
                     /locus_tag="OQM87_001514"
     CDS             complement(13106..14668)
                     /gene="trpE"
                     /locus_tag="OQM87_001514"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCS1507693.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDNALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(14690..14782)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            14941..15822
                     /gene="yciV"
                     /locus_tag="OQM87_001515"
     CDS             14941..15822
                     /gene="yciV"
                     /locus_tag="OQM87_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCS1507694.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            15819..16439
                     /locus_tag="OQM87_001516"
     CDS             15819..16439
                     /locus_tag="OQM87_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCS1507695.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            16540..17412
                     /gene="rluB"
                     /locus_tag="OQM87_001517"
     CDS             16540..17412
                     /gene="rluB"
                     /locus_tag="OQM87_001517"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCS1507696.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(17452..18042)
                     /gene="cobO"
                     /locus_tag="OQM87_001518"
     CDS             complement(17452..18042)
                     /gene="cobO"
                     /locus_tag="OQM87_001518"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCS1507697.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(18039..18797)
                     /locus_tag="OQM87_001519"
     CDS             complement(18039..18797)
                     /locus_tag="OQM87_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCS1507698.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            19017..20066
                     /gene="sohB"
                     /locus_tag="OQM87_001520"
     CDS             19017..20066
                     /gene="sohB"
                     /locus_tag="OQM87_001520"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCS1507699.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKETKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(20102..20353)
                     /locus_tag="OQM87_001521"
     CDS             complement(20102..20353)
                     /locus_tag="OQM87_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCS1507700.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            20733..23330
                     /gene="topA"
                     /locus_tag="OQM87_001522"
     CDS             20733..23330
                     /gene="topA"
                     /locus_tag="OQM87_001522"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCS1507701.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPSKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            23540..24514
                     /gene="cysB"
                     /locus_tag="OQM87_001523"
     CDS             23540..24514
                     /gene="cysB"
                     /locus_tag="OQM87_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCS1507702.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            24809..24973
                     /locus_tag="OQM87_001524"
     CDS             24809..24973
                     /locus_tag="OQM87_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCS1507703.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLTVFLGSALFWVV
                     VALLIWKVWG"
     gene            24976..25143
                     /locus_tag="OQM87_001525"
     CDS             24976..25143
                     /locus_tag="OQM87_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507704.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            25257..25352
                     /locus_tag="OQM87_001526"
     CDS             25257..25352
                     /locus_tag="OQM87_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507705.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            25516..28191
                     /gene="acnA"
                     /locus_tag="OQM87_001527"
     CDS             25516..28191
                     /gene="acnA"
                     /locus_tag="OQM87_001527"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCS1507706.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(28255..28845)
                     /gene="ribA"
                     /locus_tag="OQM87_001528"
     CDS             complement(28255..28845)
                     /gene="ribA"
                     /locus_tag="OQM87_001528"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCS1507707.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYADISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            29015..29779
                     /gene="pgpB"
                     /locus_tag="OQM87_001529"
     CDS             29015..29779
                     /gene="pgpB"
                     /locus_tag="OQM87_001529"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCS1507708.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWTLVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            29928..30236
                     /gene="lapA"
                     /locus_tag="OQM87_001530"
     CDS             29928..30236
                     /gene="lapA"
                     /locus_tag="OQM87_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCS1507709.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            30243..31412
                     /gene="lapB"
                     /locus_tag="OQM87_001531"
     CDS             30243..31412
                     /gene="lapB"
                     /locus_tag="OQM87_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCS1507710.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            31605..32342
                     /gene="pyrF"
                     /locus_tag="OQM87_001532"
     CDS             31605..32342
                     /gene="pyrF"
                     /locus_tag="OQM87_001532"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCS1507711.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNAHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            32342..32668
                     /gene="yciH"
                     /locus_tag="OQM87_001533"
     CDS             32342..32668
                     /gene="yciH"
                     /locus_tag="OQM87_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCS1507712.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(32794..33012)
                     /gene="osmB"
                     /locus_tag="OQM87_001534"
     CDS             complement(32794..33012)
                     /gene="osmB"
                     /locus_tag="OQM87_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCS1507713.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVISHQVGK"
BASE COUNT         8400 a         8002 c         8420 g         8307 t
ORIGIN      
        1 ttccacagtt ttcacctgaa gcattaaata atctgaaagg tcgagtggaa acattcacac
       61 aagatttaaa ggttatcaaa ccgtcagtaa atattcagcc tcagacaatc aatatcacaa
      121 ctcagacggt acttgatgga aaggttattg atgaaagaac cacatctcat attaatcgta
      181 tgcaggaaga taccttgatt agctcagctt acccagagga ataaacaatg ttagaactac
      241 taatatttat tgccacacat ggcaatctat taatctttat ctagtatgac gattcaacta
      301 tgaatagtcg aatgctacgt gatttcttct gagcagatac caacatcaaa aattagattt
      361 cacgtagctc acgcttaaca gaataggaat aacaacaatg tttgacacaa agcaaattag
      421 atactcaggt aaagatggta tctattttca ccttagagat aatgtagaat cttttcttgc
      481 cttaacagct actgaacaga tggactatga ctcaccgatg atggtgacaa ccaggctatg
      541 caatcggggc aaactatcac tgataatgtt cagagagcac ctaagaccat ctctattagc
      601 ggtgttgtag tggttgattc gacgggttca gttctattaa ccagacaggg gcaacttgta
      661 gagaccttta tttctacggt tgaacaatgg cgagaccaga agcaaatcat cactattgtt
      721 tgtaaagatg gaatatctat cactgatagt atcattactc agtttaaagc gagtaaagag
      781 cataccattg ctaatggtct tcgtattcag atgaccttcc aaggtgtaga atttcgttca
      841 atcgtagggc aaactgatat ttcagcagct actggcaaga ccgctaccac gaatgatggt
      901 ggtgctacca gtaaaaagaa cactggcaac actacaacga gtcttggaag ccctatgctg
      961 acctgtaaag agctttttag ctattcggca agtgagttat cagatgaagc gctaaaagcc
     1021 cgtgtaacgt gctctaagag cgttagcgtt aaaaatggtg agagcacctt cacagcctca
     1081 gctaatgagc gagcttcaaa agtgcttaag tcgggtaatg ctttacggaa gtactctgtg
     1141 aatccgaata agaagggcac ttattgagac ataaaaaggg gcttcatagc ccctgaaaat
     1201 tacagttgat aagcatcaat atccagtagc tctttatcag caatttcaaa gattggtgac
     1261 ccatcattta gcggtagcgg gtctgtaggt atggtgattt cagtgtcgtt ctgtagcgtt
     1321 atagttactg aacccttacc caatgtaagc ccttttagat gtttggacaa aattaattga
     1381 cactcgacac gacccgcttt agttgtcagg tcaatatctt taagctgggg tagttcctta
     1441 tcagtgagtg aagccttacg cttcatagta tcaatcgtgg tctgtagttc tgtgcgcttc
     1501 tggttaagtt ccttaagctg aatttttagt tcatcaatat catccatctc aagaagtagg
     1561 gcggtagctt tcttgattct ggctgtgaca atttcaacct catgctgtaa cgctgcaatc
     1621 ttcctctctg attctccatc attccccacg tcaatcatcc ccagaaaatt atcaatcatc
     1681 catcggtcaa acggtttacg cttcaacggt ggaacatcac agcgaccgtc taagtggtta
     1741 ttgcacctgt agaccccagc atacttattc tttgttccgg taggatggac actagcccca
     1801 cactgacacc taaacagacc tttgaagatg ttaatcgtta gtgggttgtc acttgcaggt
     1861 gtacggcctt tacgattctt atcaagaatc tcttgagctt tattgaacgt tgcagcatct
     1921 atagcggctt cgtagtagtt cggtatagcg gggcggtcat tgcgctgtga tgctggtagt
     1981 gtacctatcg ttgtctctga ccttaagata gtacgcacag aatgaacgct aaatccatta
     2041 gcatctttac gtcgtgagaa tgaactaatg ttattagcct gtaggtgacg tgcaatgtta
     2101 tgcagacttt caccatctag cagcagcttg aaaatcaagc gtacagtttc agcacgttca
     2161 ttgagtttaa aagtctcagg gtcaatccat ccgggaacat tccttgttaa acgttgttca
     2221 ccagcaaggg caagctttgc acgttgttcc catttctgac taacacggaa agacttctgt
     2281 ttactctcct gatgcgctat gtagctttta acccgctgaa tgattgagat tcctaaatca
     2341 tcggatagag catcccttgt gtagattagc ccatcctgta gagtgtgaat ctcgatacca
     2401 tgagcaataa tgcgattcat caaggcttct gtttcaagat gttcaaggcg gcctatgcgg
     2461 tcgatagctt cgactagtaa aattgtccct tctggaaact cccctgattc aatgcgggag
     2521 agcatatccc caaattgccc tgataagtgt ttacctttgt aaccggatag accttcatca
     2581 ttgaaagatg ccactacctg agcatcgcta ttcttcctta accactgttt gaatagctca
     2641 ttttgtcgtc tggttgaatc agcaccttca gcaccttgtg tggctgatga gaatctcaag
     2701 tatgttatta cggttctcac ggtgataaac tcccatgcaa ttgattatca tttaaagatg
     2761 aacttataaa cgttatggct aatgctacag tgccgaatag ctacaataca atgccccttg
     2821 atttacctcg ccgcttcccc tggccgacgt tactttcggt ctgcattcat ggtgctgttg
     2881 tggcgggtct gctctatacc tcggtacatc aggttattga actacctgcg cctgcgcaac
     2941 caatttctgt cacgatggtt gcgcctgctg atctcgaacc gccacaagcc gttcagccgc
     3001 caccggagcc ggtggtagag ccagaaccgg aacctgagcc gatccccgaa ccgccaaaag
     3061 aagcaccggt ggtcattgaa aagccgaagc cgaaacctaa gccaaaaccg aagccggtga
     3121 aaaaggtaca ggagcagcca aaacgcgatg tcaaacccgt agagtcgcgt ccggcatcac
     3181 cgtttgaaaa tacggcaccc gcgcgcccca catcaagtac ggcaacggct gcaaccagca
     3241 agccggttac cagtgtggct tcaggaccac gcgcattaag ccgtaatcag ccgcagtatc
     3301 cggcacgagc acaggcattg cgcattgaag ggcaggttaa agttaaattt gacgtcacgc
     3361 cggatggtcg cgtggataac gtacaaatcc tctcagccaa gcctgcgaac atgtttgagc
     3421 gtgaggtgaa aaatgcgatg cgcagatggc gttatgagcc gggtaagcca ggcagtggga
     3481 ttgtggtgaa tatcctgttt aaaattaacg gcaccaccga aattcagtaa gcagaaagtc
     3541 aaaagcctcc gaccggaggc ttttgactat tactcaacag gtaaggcgcg aggttttcct
     3601 tcaggatcaa ccgcgacata cttaaataat gcttctgtcg ctttatagcg ttgcccgatt
     3661 ggttcagacg ctactttttt cacccacact tcaatattaa tgctgaccga tgtcgtccct
     3721 ttctggacac agcgtgcata gcagcacacc acatcgccga ccgcaaccgg ccgtaagaaa
     3781 gtcattcctt caacccgcac agtcactacg cgaccgtggg caatttcttt tgccagaata
     3841 gcgccgccaa tatccatttg tgacattaac caaccaccaa agatgtcacc attggcattg
     3901 gtatcggcgg gcatggctaa agtacgtaaa acaagatcgc cctgagggac gttatgtgtt
     3961 gtagacatgg taaaaccgac ttaaaaggaa aatcacaggc gcgatgctac tatgattttc
     4021 ctgaggagaa cagaggagat acagcgccag ccccgaagga ctggcagtct ggttaggatt
     4081 tatcttcctg cggcatgtgg cggtagatat agataccgct taacaatgtg aagattaagg
     4141 taagggcggt caggccaaag actttaaagt tgacccaaat attttgcggc agccagaatg
     4201 cgatgtagat gtttgccaga ccgcaaagga taaagaaaac agcccaggcc agatttagct
     4261 tcgaccatac cggttgcggc agcgtgagtt ctttacccag catccgctga attagcggct
     4321 ttttcatcac ccattggctg actaacaggg ctaccgcaaa cagagcataa atgactgtaa
     4381 ccttccattt aataaactca tcattgtgga agaacagcgt caagccaccg aagacgacca
     4441 ccagaacaaa agtgatcagg gccatcttct caaccttacg aaagcgaacc cagctataga
     4501 taagcacaat cgccgtggcg acgatcagcg ctgcagtagc cgcatagatg tcataaatct
     4561 tgtaaaacgc gaaaaagaca accagcggta aaaaatcaag aaactgcttc attttacgat
     4621 tccgtaatca aatgcttaag gttattggcg aatcaacata tacaggcgga acagatagat
     4681 gagcaatatg gcagaaatca ggttgctcaa ggtgttcgcc agtacggcac caatttccgg
     4741 ggttaatgcg gcaaaagaag aggcaaaaag cagcaacagt gtttttgcca gcaaccagct
     4801 cagtactgcg ggtgccacca gacgcatatt cgcccaagtc agccgcatac tgctacgcat
     4861 cgaggcaaaa atgcccattt tgtcctgtac cagcatcacc ggtgccaggg ccagtaaaat
     4921 ggccatgata attcccggaa cgaccaccag catgatgcca atctgtacta aaagggtagt
     4981 cagaaaaatc agaataaata acttgggcaa tatcggcgca ctggcaccaa tagctcgcag
     5041 cgcacagact ctctgacccg cagacaccag ctggataatt aatattacgc ctccggcgag
     5101 aatggcgtta ccgattaatt ctgaaaaagt ggacgccgct gaagcctgca gcaaaatttg
     5161 ttgctgttcc ggtgacatat tctgaaccag gtcgaacaac ccactactgc cgctaacggg
     5221 cacgccgtca ttgagctgcg caagctgtgc atcactgggt gagaaaacat gccctaacac
     5281 cactgtgata aacgcacata gcaacgatac caacagaatg gtcataaatt gattacggaa
     5341 gaaatttccg gtgtcacggt atacggactg cgccgtgata gacatgcact ctccttgagt
     5401 tttatgcagg tgttaattag cgggcaattg taccctggtt aagcctcggg tggcagcatc
     5461 aggtcttgta tggaaaagca tatctttgta aagcggaggt aattgctggc cttcaggcgt
     5521 gggcaaaccg cggcaagtgg cgatcgagtt aatatttgcg tagagaaaat atttaaaaat
     5581 tgatttaaat cacattaacc aggattctca atgcaacttc taaattaatc cagatcaata
     5641 aatggtgaat tatcatatgt aatgtgatct atgtaggatc atttgttact ccaatgtagg
     5701 tatattcgtc acgtttttat aaccataacg acggagcgga tatgaaaaag ttaacagtgg
     5761 cggctttggc agtaacaact cttctttctg gcagtgcctt tgcgcatgaa gcaggcgaat
     5821 tttttatgcg tgcaggttct gcaaccgtac gtccaacaga aggtgctggt agtacgttag
     5881 gaagtctggg tggattcagc gtgaccaata acactcaact gggcttgacg tttacttata
     5941 tggcgaccga caacattggt gtggaattac tggcagcgac gccgttccgc cataaaatcg
     6001 gcacccgggc gaccggcgat attgcaaccg ttcaccatct gccgccaaca ctgatggcgc
     6061 agtggtattt tggtgatgcc agcagcaaat tccgtcctta cgttggggcc ggtattaact
     6121 acaccacctt ctttgataat ggatttaacg atcatggcaa agaggcgggg ctttccgatc
     6181 tcagtctgaa agattcctgg ggagctgccg ggcaggtggg ggttgattat ctgattaacc
     6241 gtgactggtt ggttaacatg tcagtgtggt acatggatat cgataccacc gccaattata
     6301 agctaggcgg tgcacagcaa cacgatagcg tacgcctcga tccgtgggtg tttatgttct
     6361 cagcaggata tcgtttttaa ttccgcacaa aaacgacccc gtaatatacg gggtcaataa
     6421 ggacatggta taaagggtat tatttcttcg cttctacgca atcagtttca gaacgaatta
     6481 aaaatttctc agttgtttgc ggaatatgct gaatcagcca gtcggccatt tgcttttcct
     6541 catttaaaat cgcttcgatg atcggaattg agactgtatc accggcattt tttgctgctg
     6601 ctaatagtga ggtgtaacag gcgatttcaa attgctcgaa gacatatccg ctaatagagc
     6661 ctttaactat ttcatcagaa gggaatatac caccgattga ctgcccaagc gcagccattt
     6721 tactcatgga atctttaatg actgaacgtg aaatgtcatt acgatcaaga atagtttcca
     6781 gttgaacaat ctggtttttg gtttcactaa gatgttgttc aatacgagcg cgtagttcag
     6841 gataattatc tatacggctg gccatggatt caagcataga ttcggcttgc ttttccattg
     6901 cgtgggcgtc acgtaaccag tcatgataat gttcaatacg attcatgaaa atgtcctctt
     6961 tataaaatga ctaaaagtta aaattcatat ttcaggcttt attttcggct ttcttattta
     7021 cgttattaag ggccagatca gtcagtttga tgtcggtggc cttttcttct tccagggttt
     7081 ctttcagaag cttcgctgct ttacggtaac ctaattgttc agccagcgtc gctaatgtcc
     7141 cgtaactggc aatctcataa tgctcgactt tctgtgctgc ggcaatcagt gcggcatcac
     7201 gcacttcgtt tttctcggta ctttcgatga cctcattagc ttcttcaata agaccttcca
     7261 ttgccacaca tttcatgcgc ttaattttca gatttgattt tgattccaca acttgatcaa
     7321 ttcgttcaat ctgtccatga gtttcctcga ggtgcgcatg aaaagcctga cttaattttt
     7381 catttgatgt tgctcttgcg agttttgcca gtgcccgggg taattgtttt tctgcgctgt
     7441 aggtatctga aagcagttga ataaatacat cttcaatggt cttcatattc atatttttct
     7501 ccagtgaaat cactgcgagc catacggccc gcagatgcaa ttatctttat cagctgatat
     7561 taataattaa cggtgaatca ggatttgtct gatttattac caccgctttg ttgaccgcct
     7621 tttttacccg cttcagatgc gcgttgcgga tcatttttaa aattaccgcc gctatgctga
     7681 ccgcctttac ggcctgcgtc ggatgccttc tcacggtctt cggcgaaatt tcctgaacca
     7741 ccacgatgtt cggccatgtt atttctcccg ttgcgttgca ttgtttcatt aatatgagtg
     7801 ttgtgtgtcg acactcatta aaattagtcg ctaatgagaa ttagtcaaat taagcgcaac
     7861 gagaagatag agggaaaata tattttgagg aacattctgg atatattaac aattacctga
     7921 ggaataaatg acttagagaa aattattgat tttactggtg ttatgttgcg ggattaattt
     7981 gtttgattat attactgttg ggcggaaaaa tgacgtaagt tgacgttcga ccggggtaag
     8041 cgaaacggta aaaagataaa tattaaatga atttaggatt tttccggctt cattaaagaa
     8101 agttaaaatg ccgccagcgg aactggcggc tgtgggatta actgcgcgtc gccgctttca
     8161 tcggttgtac aaaaactttc agtgccgcca gcattttctc tggctcatta atatgttgct
     8221 cgatgatttt aacaatggcc gaaccagaaa tcgcgcccgc agctcctgca tcaatcgctg
     8281 cttttacctg atccggggcg gaaataccaa acccctgcaa tggaggtgca gcgttgtact
     8341 ctttcagctt cgcaaccaga tgattgaggg gtaacgcggc gcggttttct gcgccggtca
     8401 cgcccgctcg tgacagcaaa tatgtgtaac cacgaccgta agaggctatc tggcgcagta
     8461 ggtcgtcatc ggcatttggc gggcagatga agataggtgc gacattatgg cgcaacgcgg
     8521 cctggcggaa gggcgcggac tcttcaactg gcacatcggc aaccagcacc gaatcgacgc
     8581 cgactttctc gcactgggca taaaactcat caatgccttt gttaaacacc agattggcat
     8641 acatcaacag gccaatggga atggtcgggt gtttctggcg aatcagtgcc agcatttcaa
     8701 aacattgtgc cggagtcaca cctgccgcaa aggcgcgcag agtggcgttt tgaatggtcg
     8761 ggccatccgc cagtgggtcg gagaagggga tacctaactc cagcgcgtca gcaccggctt
     8821 caattagcgt atcgataatt ttcaatgact gctcaatgcc cggatcaccg agcgtgacga
     8881 aaggaatgaa tgcgccttct ttgcgctcct tcaactgggc aaacagagat tcgtagcgtt
     8941 ccatcagatt tcccctcgtg ctttcaaaat atcgtgaacg gtgaagatgt ctttatcgcc
     9001 gcgaccggaa aggttaacca ccagtagctg ctctttatcc gggttttcgc gcatcatttt
     9061 cagcgcatgg gccagggcgt gggaggattc cagcgccggg atgatccctt cgtgcaggca
     9121 cagcgttttg aaggcttcaa gggcttcatc atcggtaata gacacgtaat cagcgcgtcc
     9181 agtgctgtta agatacgcgt gttgtgggcc gacagacggg aaatccagtc cggcggagat
     9241 ggagtaagat tcttcaatct gcccgtcttc ggtttgcatc atcggcgctt tcataccgaa
     9301 ataaatgccc acgcgaccat gttttagcgg tgcgccgtgc tcgccagttt cgataccgtg
     9361 accaccaggc tccacaccaa tcaggccgac gttggtttca ttgatgaaat cagcaaacat
     9421 gccgatggca ttcgaaccgc cgccaacaca ggcgataacg gcatccggca ggcgaccttc
     9481 tctttccaga atctgcgctt tggtttcttc gccaatcatc cgctgaaact cacggacaat
     9541 ggtcggataa ggatgcgggc cagccgcggt gcccagcata tagtgcgcgg tttcataact
     9601 gccggaccag tcgcgtagcg cctcattaca ggcatctttc agcgtggcgg aaccgctatg
     9661 taccgggatc acttccgcac ccattaagcg catacgaaaa acgttaggcg actggcgttc
     9721 aacgtctttg gcacccatat aaatacggca tttcaggccg agcagggcgc tggcaagggc
     9781 cgacgccacg ccatgctgac cggccccggt ttcggcgatg atttcggttt tacccatccg
     9841 cttcgccagc aacgcctgcc ccagcacctg gttggtttta tgtgcgccgc cgtgcagcaa
     9901 atcttcacgc ttgagataca gcgtggtgtt cgtcccggct gtaatgttct ggcatttggt
     9961 cagtgcggtt ggacgcccgg catagttttt cagcaggtcg ttgaactggg cttgaaattc
    10021 aggatctttt tgcgcgctga caaaagcttc ttccagctgg cgcagagcag gcatcaggat
    10081 ttgtggcacg tacatgccgc caaactcacc aaaatagggg ttaagtaatg ttgtcattgt
    10141 tcctttcctt aatatgcgcg cagcgtctgg aaaaccgagg ccaaaagacg tgcgtctttg
    10201 atgcccggtt gcgactctac agcagaatta aaatcaagtc cggcgcagcc ggtttgtgcc
    10261 gcttccacgc agttatctgc gcctaagccc cccgccagca gaacgttgcc aagcgattga
    10321 ccatttaata gtgaccagtc aaaacgttgc ccgcttccac cctggccgtt gtctaaaaca
    10381 tatttatcaa cgtgctgaaa ctcgcgggcg ggcagggttt caccgacgct taatgctttc
    10441 cagatggcaa catgtgctgg cagagcttca cgcagcgtat cgatatacag ctgatcttca
    10501 ttaccatgca gttgcactgc cgccagcgat aacaccttag ctttgtccac cacatcggca
    10561 atatcgtgat tgcggaacac gccaacatac tgcaacggtg ccgcagccat cacttcctgc
    10621 gcctgttcaa cgttgacgca acgcggtgat gtcgcaacaa aaatcaaccc accgtaaatc
    10681 gcgcccgcgt cataagctgc tttagcatct tgcccacgcg tcaggccaca tactttattc
    10741 tcacccagca acacccggcg cacggcggcg tgcaaatcgt catgggccat caacgccgaa
    10801 ccaatcagaa aaccgttagc gaagtggctt aactcgcgca cctgagcgta agtattgatg
    10861 ccggattcgc tgattaccgt tacgttgtgc cccagtttcg gcgcaagctc gcgggtacgg
    10921 ttgagatcaa tcgacaaatc acgcagatcg cggttgttga tgccaacgac ctttgcccct
    10981 aatgcaatgg cgcgctccag ttcctcttca ttactgactt cggtcagcac ccccatctcc
    11041 agactgtgag cgacggctgc aagctggcga tattggtcgt catccagtac tgaaagcatt
    11101 aataagcagg catcggcctg gtaatagcgc gccagataga tctggtaagg gtcgataatg
    11161 aagtctttac ataaaatcgg ctgcggggcg atttggctga cgatgaggag gaaatcaaag
    11221 ctcccctgaa aatatttctc atcagtcagc accgaaattg ccgaagcgta atgtttataa
    11281 atggcggcaa tgcgtgccgg atcgaaatca tcacggatca cgccttttga cggcgacgct
    11341 tttttacact ccagaataaa cgccgtgcgt gcaccttgta gcgcatcata aaaatgtcgt
    11401 gtgctcggct gaacctcatt ctgaaaactg gccagcggtt gctgctgttt gcgggtttct
    11461 acccaaatcg ccttgtctgc gacgattttc gctaaaacgg tttgcatcat ttaccctcgt
    11521 gccgccagcg cggtaactct gtcgtaagcg gaaccactgc gcagtacctc aagaacggtt
    11581 tgcgcattgg cttgcagatc ttcatggcca tgcaggcgca ttaacatggc gacgttcgca
    11641 gcgacggctg cttcatgggc ggcgtcgcct ttaccttgta acaagcgtgt taaaatgtca
    11701 cggttttctt caggtgttcc gcctgccagt tgctcctggt ggtagggagt caggccaaaa
    11761 tcttcagcgg tcaattgata gctcttaatt ttgccgtcat gcagctcggc aacgattgtc
    11821 ggcgcgtgta atgaaacttc atccatcccg ccgctgtgca ccaccgccgc gcgttgatac
    11881 cccagcacgc gcaaggtttc ggcaatcggc agcaccagtt ccggactata aacaccaatt
    11941 aacgccagcg gcggatgcgc cgggttaatc aatggcccca gcacattgaa cagggtgcgg
    12001 gttttcagtt gctggcgaac cggcatcgcg tggcggaatc cggtgtgata tttcggcgca
    12061 aagaggaaac atacgcctaa ctcgtccagc gcctggcgcg atttattggc gttcatatca
    12121 agattaatac cgaatgccgc cagcagatcc gacgagccgg atttactgga gacgctgcgg
    12181 ttaccgtgtt tcgccacttt cagcccacag gccgcggcga caaacgcact ggcggtagaa
    12241 atattgatac tgttgctgcc gtcaccgcca gtaccgacga tatcagcaaa cagataatcc
    12301 gggcgcggga acggcgctgc gttttccagt agcgcggttg ctgccccggc gatctcgttc
    12361 gggtgctcac cacgaatttt catgctcacc agcgccgccg ccagttgttc cggcttcagc
    12421 tcgccacgca ccaccgctga aaacagctgg tggctttctt gttggctaag cgtctgcgcc
    12481 tgatacagtt tttccagaat cggttgcagc gtgttggctg gctctagttt ctgctgcgcc
    12541 caggccagcg tttgttccag caggcgagcg ccctgggtgg tgagaatgga ttccggatgg
    12601 aactggaatc cacaaacgcg atccgcatcg tgacgtactg ccatcaccat gccattaaaa
    12661 tgggcgttga tggttaaacc ggccggaatg ttactgccaa ccagcgagtg ataacgcgcc
    12721 accggcagcg ggtttgttaa tccggcaaac atcgcctgac cgtcatgttc aatgctcgac
    12781 gctttaccgt gaagaatttc gcccgcctga ccgacatagc ccccgtaagc ttcgacaatt
    12841 gcctgatgtc cgaggcaaat gccaataatg ggcagcttgc cacgcaagcg ggtgaggagt
    12901 tccggcatac aaccggcttc gctcggcaca ccggggccag gagaaagcat cagtaccgga
    12961 ttgctcatcg tcgccaggcg ttcaattaag gtctgcgccg gaatatggtt gcggtaaatc
    13021 accacgttat gaccattgct gcgcaactga tctgccaggt tgtacgtaaa agagtcgata
    13081 ttatcgagca gcagaatgtc agccatcaga aagtctcctg tgcatgatgc gcggtggcaa
    13141 tagcgcgcag tacagcgcgg gctttgttac gggtttcgtc ggcttccgac tgcggaacag
    13201 aatcaaggac tacaccagca cccgcttgca cggtggcgat accgttttcc accagcgccg
    13261 agcggatcac aatgcaggtg tcgagatcgc catgcgcggt gaaataacct accgcgccgc
    13321 cgtagctgcc gcggcgacga ccttccgcct cggcaattaa ctgcatagcg cgtactttcg
    13381 gcgcaccgct taacgtcccc atattcatac aggcgcgata agcgtgcagg gcgtcaagat
    13441 cgtgacgcag ttcgccgact acgcgagaga cgaggtgcat cacataggaa taacggtcaa
    13501 ctttggtgag atcggcgacg tagcggctgc caggggtgca aatgcgtgcc agatcattac
    13561 gggcgagatc aaccagcatc agatgttcag acagctcttt atggtcagta cgcatttcca
    13621 gttcaatacg gctgtcgaga tctctgtcca gtgaaccatc ggcgcgacga ccgcgtgggc
    13681 gtgttccggc aatcgggtag atctcaatct ggcggctggt ggcatcatac ttgagcgagc
    13741 tttccggcga cgcgccaaat agggtgaaat cattatcctg cataaaaaac atgtacgggc
    13801 tgggattact ctttttcagc acgtaatagg ccgccagcgg tgacgggcag ggcagagaga
    13861 aacggcgaga tggcaccacc tggaaaattt ctccagcgcg aatcgctttt tgcaacaaac
    13921 gcactacgcc accgaactct tcatcgctct gattacattc acaacgcata tgcggcacgg
    13981 aaaccactgg cagcggcggc gcggcttcgg tcagttgctg acgtagttcg ttcaggcgag
    14041 cagtgagacg ttgtttttct tcttcattcg gagcaaacag gctggcctga atacgggtgc
    14101 tttttttctg atggtcaatc accatcagcg tttcagcgag ataaaaacag aaatcagggc
    14161 agttattttc cgctgacagt tgcggtaaat cttcaaatcc cgccacaagg tcataagaga
    14221 acaggccgcc gaagaacatt gcttctcgtt cttccttcgg tacattcaac agattctgca
    14281 ataaacggaa agcgtcaaaa accgaaaggg agcataagcg ggcgtcttca tccagcagtg
    14341 gactgacagg ggggaagcgc agcacacggc agtttggtga ttgttcactt tccacacccg
    14401 caggcagggc gttatccagt agtgccagca gggcttcgcc attgccggaa agtgcctgaa
    14461 ttgtgacggt gtcacctaat gctgtaatgt gcagcgcatt gtctatcagc agcaggcttt
    14521 ttaaatcatc tttgctgtcg atatctgcgg attccagcag cagcgttgcc ggacgattcc
    14581 cacacaactg gtgaaaaagc gcagtcgggt tgtcgcgata agcgccttcg caggttagca
    14641 gttcgagagt cggtttttgt gtttgcattg ttattctcta attttgttca aaaaaaagcc
    14701 cgctcattag gcgggctggg tatctgattg ctttacgcat ggtgaataca ctgcccgttt
    14761 caggaagtgg ccaccaacct ttcagtacga aaattgcttt cattgtcgat accattttta
    14821 cgtgaacttg cgtactagtc aactggttcg atgattaatt gtcaacagct catttcagaa
    14881 tatttgccag acccgttatg atgtcggcgc aaaaaacatt atccagaacg ggagtgcgcc
    14941 ttgagcgaca cgaattatgc agtgatttac gacctgcaca gccataccac agcttccgat
    15001 ggctgcctga cgccagaagc attggtgcac cgtgcagtcg agatgcgcgt cggcaccctg
    15061 gcgatcaccg accatgacac cacagctgca atcgcgccag caagagagga aatttcacgt
    15121 tcgggactgg cgctgaatct tattcccggc gtggaaattt ccacggtctg ggaaaatcat
    15181 gaaattcata ttgtcgggct gaatattgat attactcatc cgctaatgtg tgagttcctc
    15241 gcgcagcaga cagaacggcg caatcagcgg gcgcagcaaa ttgccgaaag acttgaaaaa
    15301 gcgcaaatac ctggggcgct ggaaggcgca caacgactgg cgcagggcgg cgcagtgacg
    15361 cgcggtcatt ttgcgcgttt ccttgtggag tgcggcaaag ccagttcaat ggcagatgtc
    15421 tttaaaaagt atctggcgcg cgggaaaacc ggatacgttc cgccacagtg gtgtacaata
    15481 gaacaagcta ttgatgtcat tcatcattct ggcggtaagg cggtgctagc tcatcctggg
    15541 cggtacaatc ttagtgctaa atggctgaaa agattggttg cgcattttgc cgaacaccac
    15601 ggcgacgcga tggaagtcgc gcagtgccag caatcgccca atgaacgcac ccagctggcc
    15661 acacttgcgc gtcagcatca tttatgggca tcacaaggat ctgattttca tcagccatgc
    15721 ccgtggatcg aactgggtcg taaactctgg ctgcccgcag gcgttgaagg cgtctggcag
    15781 ctatgggaac agccgcagaa caccacagag agggaattat gagccagttt ttttatattc
    15841 atcctgataa cccgcagcaa cgtctgatca accaggcggt ggagatcgtg cgtaaaggcg
    15901 gggtgattgt ctatccaact gattccggct atgcgctcgg ctgtaaaatt gaagataaaa
    15961 acgccatgga gcgtatttgt cgtattcgcc agctgccgga cggtcacaac tttaccctga
    16021 tgtgtcgcga tctttctgaa ctctcgacct attcattcgt ggataacgtt gcgtttcgtt
    16081 tgatgaaaaa caacacgccg ggcaactata ccttcatcct gaaggggacg aaggaagtgc
    16141 cacgccgtct gttgcaggag aaacgcaaaa ccatcggtat gcgtgtgcct tctaacccga
    16201 ttgcgcaggc gttgcttgaa gcactgggcg aaccgatgct ttccacttcg ctaatgctgc
    16261 caggcagcga atttaccgaa tccgatcccg aagaaattaa agaccgtctg gaaaaacagg
    16321 tagatttgat tattcacggc ggctatctcg ggcagaaacc aacaacggtt attgatctta
    16381 ccgatgatac gccggtcgtg gtgcgtgaag gcgtaggtga tgtgaagcct ttcttataag
    16441 tgcgtaactc tgtatactac gcggccttaa aatggcgcta tacggcgcca ttgattctcc
    16501 tgttcgacgc ctgtgaaggc gacaccaaaa ggaagctcta tgagcgaaaa attacagaaa
    16561 gtgctggccc gcgctggcca cggctcccgc cgtgaaatcg aatctattat tgaagccggt
    16621 cgtgtgagtg ttgatggcaa aattgctaaa ctcggcgatc gcgttgaagt tacccctggg
    16681 ctgaaaattc gtatcgatgg tcatctgatt tcggtacgtg agtccgctga acaaatttgt
    16741 cgcgttctgg cctattacaa accggaaggt gagttgtgta cccgtaacga tccggaaggg
    16801 cgtccgacgg tgtttgaccg tctgccaaag ctgcgtggcg cacgctggat tgccgtaggt
    16861 cgtctggacg ttaacacctg tggtctgctg ctgttcacca ccgatggtga actggcaaac
    16921 cgtttaatgc atccaagccg tgaagttgaa cgtgaatacg ccgtgcgtgt atttggtcag
    16981 gttgacgacg cgaaactgcg tgatttgagt cgtggcgtgc aactggaaga tggtccggca
    17041 gcttttaaaa ccatcaagtt cagcggcggc gaagggatca accagtggta caacgtgact
    17101 ctgaccgaag gccgtaaccg tgaagttcgt cgtctgtggg aagcggttgg tgtgcaggtg
    17161 agccgcctga tccgtgttcg ttacggtgat atcccactgc caaaaggtct gccacgcggt
    17221 ggctggacgg agctggacct cgcccagact aactatctgc gcgaactggt ggagcttccg
    17281 ccagaaacca gctctaaagt cgctgtagaa aaagaccgtc gtcgcatgaa ggctaatcag
    17341 attcgtcgtg cggtgaaacg tcacagtcag gtgagcggcg gtcgtcgttc tggcgggcgt
    17401 aataacggtt aatcaaagta tgccggacgt catatccggc atttttacag attaataatc
    17461 aatccctatt tgcgccttaa caccggcatc aaacgcatgt ctgatggggc gcagctcact
    17521 taccgtatcc gccagttcaa gaatatcccg atgacagcct cgacccgtga taatcaccgt
    17581 ctgttgatgt ggacgttcat ttaacgcctg caccacttct tccagtggca aatagtcata
    17641 cgccaccata tacgtcagtt catcaagcaa aaccatatcc agtgaggaat cagcaagcat
    17701 ccgctttgca tgttgccaga cttcgcggca ggcggcggta tcagactcgc ggttttgtgt
    17761 atcccaggta aagcccgttg ccatcacctg aaactcaacg ccatgtggct ccagcagatt
    17821 gcgttcgcca ttaggccagg tgcctttaat aaactgcacg acgcccactt tttttccgtg
    17881 accaactgcg cgtgttgccg taccaaatgc cgcggtggtt ttgccttttc cattgccggt
    17941 aaagacgatg ataataccgc gttcatcctg ggcctgggcc acacgagcat ctactttttc
    18001 tttcactcgc tgctgacgct gttggtagcg ttcatcactc attgggaaat tcctggttta
    18061 cggcccggtt gggcgtcaaa ggtcatgccg gttttacggc ggctgtcatc gcccatcagc
    18121 cagaggtaga gcggcatgat atcagcgggt gttttaagtt tctgtggatc ttcggtcggg
    18181 aaggcgcttg cgcgcattgc ggtgcgcgta ccgcctgggt taatgcagtt gacgcgcagg
    18241 cgctgctgat attcatcggc cagtacctgc atcatccctt cggtggcaaa tttcgacgct
    18301 gcatatgcac cccagttggc tcgtccctga cgtccaacgc ttgatgaagt aaagaccagt
    18361 gaaccggcgt ccgatttgag taataaagga agcagtgcct gggtgagcat aaaggtggca
    18421 ttaacgttga cctgcatgac gtcctgccag acctgcggat tttgttcgct cattgggcaa
    18481 acatcgccga gcaatccggc attatgcaaa acaccatcca gacgcggata attaacgaca
    18541 atgcgctgtg ccagttgttg gcaattttcg gacgtgcagg tcagcaaatc gagaataaac
    18601 cactgtggct gacgcccagt ttcttcgttt atgtggctgg ctacctgacg taatttttct
    18661 tcattacggc ccaacagaat cactgtcgca ccatagcgtg catacgtcat cgcggcttca
    18721 cgaccaatcc catcgctggc tcccgtcacc aggataatgc gatcattgag taaatcttgt
    18781 tttggctggt aatgcatggc tactcctcaa cgacgttgtc tgtcgcgctg ccagtgtact
    18841 ttatgacttt ggggctttat gcctgaaaca gaagggcatt tcaatcagcc tggggcgata
    18901 acaccgcaaa atggatactt tgtcatactt tcgctgcaat aacatctctg cgagacggct
    18961 taacatgcct gttgtaaact gtgagccaaa gcgttgttta accaaggtgg ggactcgtgg
    19021 aattgttgtc tgaatatggt ttgtttttgg ctaaaatcgt taccgttgtg ctagcgattg
    19081 cggcgattgc cgccattatt gtcaatgttg ctcaacgtaa taaacgccag cgtggcgagt
    19141 tacgggtcaa caatctcagc gaacagtata aggagacgaa agaagaactg gccgcggcgc
    19201 tgatggactc acatcagcaa aaacagtggc acaaagcgca gaagaaaaag cacaagcaag
    19261 aagcgaaagc agcaaaagcg aaagccaaac tgggcgaggt ggcaactgac agtaaacctc
    19321 gcgtctgggt gctggatttt aaaggcagca tggacgccca tgaagtgaac tcgctacgtg
    19381 aagagataac ggcggtactc gcagcattca aaccgcagga tcaggttgtg ctccgtctgg
    19441 aaagccctgg tggcatggtg catggttacg ggttggcggc ttcgcagctg cagcgtctgc
    19501 gtgataaaaa cattccttta actgttacgg tagacaaagt cgctgccagc ggcggttaca
    19561 tgatggcctg tgtggcggac aaaattgttt ccgcaccgtt tgctattgtg ggttccattg
    19621 gggtggtggc gcaaatgccc aactttaacc gcttcctgaa aagcaaagat attgatatcg
    19681 aactgcacac cgccgggcag tataagcgca cgctgacctt gctgggtgaa aataccgaag
    19741 aagggcggga gaaattccgc gaagagctga acgaaacgca tcagttattt aaagattttg
    19801 tgaagcgtat gcgtccgtct ctggatattg aacaggtggc aacgggtgaa cactggtacg
    19861 gacaacaggc ggtagagaaa ggcctggttg atgaaatcaa caccagtgat gaagttattc
    19921 ttagcctgat ggaaggccgt gaagtggtta atgtacgcta tatgcagcgt aaacgactca
    19981 ttgaccgatt caccggcagc gcggcagaga gcgccgatcg attgttgcta cgctggtggc
    20041 agcggggtca aaagccattg atgtaaaaga caaacgcgag gctaagacct cgcgttttgc
    20101 tttaatcaac cagatgatat ttttctgaaa gcacatgggc caggtgtttg aacatattaa
    20161 acaccgcggt gcttttggct gttggcaatc cttgttcatc taaaaagtag tcgccggtaa
    20221 ataccagcac gccattacgc tgcgtgacgc cggtggcttc aatccctgcg agcgtatcct
    20281 catgctcacg aatgattttg ttagcctctt tcaacagcgt ttcgcggtcg atgggttgtg
    20341 tctctttgtt cattatttac tccttaaaca aggacattag tctacgccag gcatggcttg
    20401 cagacaaata taccacgctg gtggcaagag cgccttactg gcaactttgg attttgcatg
    20461 ctaataaagt tgcgtatcgg attttatcag gtacagtgtg acgctttcgt caatctggca
    20521 atagatttgc ttgacattcg accaaaattc cgtcgtgcta tagcgcctgt aggccaagac
    20581 ctgttaactc agtcacctga attttcgtga acagagtcac gacaaggggg tgatatccgc
    20641 agagagcgag tccatatcgg taactcgttg ccagtggaag gtttatcaac gtgcgacgca
    20701 ttcctggaag aatcaaatta ggtaaggtga atatgggtaa agctcttgtc atcgttgagt
    20761 ccccggcaaa agccaaaacg atcaacaagt atctgggtag tgactacgtg gtgaaatcca
    20821 gcgtcggtca catccgcgat ttgccgacca gtggctcagc tgccaaaaag agtgccgact
    20881 ctacctccac caagacggct aaaaagccta aaaaggatga acgtggcgct ctcgtcaacc
    20941 gtatgggggt tgacccgtgg cacaattggg aggcgcacta tgaagtgttg cctagtaaag
    21001 agaaggtcgt ctctgaactg aaacaactgg ctgaaaaagc cgaccacatc tatctcgcaa
    21061 ccgaccttga ccgcgaaggg gaagccattg catggcacct gcgggaagtg attggtggtg
    21121 atgatgcgcg ctatagccga gtggtgttta acgaaattac taaaaacgcg atccgccagg
    21181 catttaacaa accgggtgag ctgaatattg atcgtgttaa tgcccagcag gcgcgtcgct
    21241 ttatggaccg cgtggtgggg tatatggttt cgccgctgct gtggaaaaag atcgctcgtg
    21301 gtctgtctgc cggtcgtgtg cagtcggtgg cggtccgcct ggtggtagag cgtgagcgtg
    21361 aaattaaagc gttcgtaccg gaagagttct gggaagtcga tgccagcacg actacgccat
    21421 ctggtgaagc gttggcgttg caggtgactc atcagaatga caaaccgttc cgtccggtca
    21481 acaaagaaca aactcaggct gcggtaagtc tgctggaaaa agcgcgctac agcgtgctgg
    21541 aacgtgaaga caaaccgaca accagtaaac ctggcgctcc ttttattacc tctacgctgc
    21601 aacaagctgc cagcacccgt cttggttttg gcgtgaaaaa aaccatgatg atggcgcagc
    21661 gtttgtatga agcaggctat atcacttaca tgcgtaccga ctccactaac ctgagtcagg
    21721 acgcggtaaa tatggttcgc ggttatatca gcgataattt tggtaagaaa tatctgccgg
    21781 aaagtccgaa tcagtacgcc agcaaagaaa actcacagga agcgcacgaa gcgattcgtc
    21841 cttctgacgt caatgtgatg gcggaatcgc tgaaggatat ggaagcagat gcgcagaaac
    21901 tgtaccagct aatctggcgt cagttcgttg cctgccagat gaccccagcg aaatatgact
    21961 ccacgacgct gaccgttggt gcgggcgatt tccgcctgaa agcacgcggt cgtattttgc
    22021 gctttgatgg ctggacaaaa gtgatgcctg cactgcgtaa aggcgatgaa gatcgtatct
    22081 taccggcagt cgataaaggc gatgctctga cgctcgttga actaacacca gcccagcact
    22141 ttaccaagcc gccagcccgt ttcagtgaag catcgctggt taaagaactg gaaaaacgtg
    22201 gtatcggtcg tccgtctacc tatgcgtcga tcatttcgac cattcaggat cgtggctatg
    22261 tgcgagtaga aaatcgtcgt ttctatgcgg aaaaaatggg cgaaatcgtc accgatcgcc
    22321 ttgaagaaaa tttccgcgag ttaatgaact acgactttac cgcgcagatg gaaaacagcc
    22381 tcgaccaggt ggcaaatcac gaagcagagt ggaaagctgt actggatcac ttcttctcgg
    22441 atttcaccca gcagttagat aaagctgaaa aagatccgga agagggtggt atgcgcccga
    22501 accagatggt tctgaccagc attgactgcc cgacttgtgg tcgcaaaatg gggattcgca
    22561 cagcgagcac cggggtattc cttggctgtt ctggctatgc gctgccgccg aaagagcgtt
    22621 gcaaaaccac cattaacctg gtgccggaaa acgaagtgct gaacgtgctg gaaggcgaag
    22681 atgctgaaac caacgcgctg cgcgcaaaac gtcgttgccc gaaatgcggc acggcgatgg
    22741 acagctatct cattgatccg aaacgtaagt tgcatgtctg tggtaataac ccaacctgcg
    22801 acggttacga gatcgaagag ggcgaattcc gcattaaagg ttatgacggc ccgatcgttg
    22861 agtgtgaaaa atgtggctct gaaatgcacc tgaaaatggg gcgtttcggt aaatacatgg
    22921 cctgcaccaa cgaagagtgt aaaaacacac gtaagatttt acgtaacggc gaagtggcac
    22981 caccgaaaga agatccggtg ccattacctg agctgccgtg cgaaaaatca gatgcttatt
    23041 tcgtgctgcg tgacggtgct gccggtgtgt tcctggctgc caacactttc ccgaaatcgc
    23101 gtgaaacgcg tgcgccactg gtggaagagc tttatcgctt ccgcgaccgt ctgccggaaa
    23161 aactgcgtta tctggccgat gcgccacagc aggatccgga aggtaataag accatggttc
    23221 gctttagccg taaaaccaaa cagcaatatg tctcttcgga aaaagacgga aaggcgactg
    23281 gctggtcagc attttatgtt gatggcaaat gggttgaagg aaaaaaataa cctttaattc
    23341 tatcaggttt ttataaacaa agggtcgcga aagcggccct tttttattgc atattatttt
    23401 ttcttcacac ctatacacta aggctataaa tgatatagtg gttatagtta gcaccttttt
    23461 tattattaaa tcgtattagt cacccgccag gtgtgacgaa aaaacgatgt tctgatggcg
    23521 tctaagtgga tggtttaaca tgaaattaca acaacttcgc tatattgttg aggtggtcaa
    23581 tcataacctg aatgtctcat caacagcgga aggactttac acatcacaac ccgggatcag
    23641 taaacaagtc agaatgctgg aagacgagct aggcattcaa attttttccc gaagcggcaa
    23701 gcacctgacg caggtaacgc cagcaggaca agaaataatt cgtatcgctc gcgaagtcct
    23761 gtcgaaagtc gatgccataa aatcggttgc cggtgagcac acctggccgg ataaaggctc
    23821 gctgtatatc gccaccacgc atacccaggc acgctacgcg ttaccaaacg ttatcaaagg
    23881 ctttattgag cgttatcccc gcgtttcttt gcatatgcac cagggcccgc cgacacaaat
    23941 tgctgatgcc gtctctaaag gcaatgccga tttcgctatc gccacagaag cgctgcatct
    24001 gtatgaagat ttagtgatgt taccgtgcta ccactggaat cgggctattg tagtcactcc
    24061 ggatcacccg ctggcaggca aaaaagccat taccattgaa gaactggcgc aatatccgtt
    24121 ggtgacatat accttcggct ttaccggacg ttcagaactg gatactgcct ttaaccgcgc
    24181 agggttaacg ccgcgtatcg ttttcacggc aacggatgct gacgtcatta aaacttacgt
    24241 ccggttaggg ctgggggtag gggtcattgc cagcatggcg gtggatccgg tcgccgatcc
    24301 cgaccttgtg cgcgttgatg ctcacgatat cttcagccac agtacaacca aaattggttt
    24361 tcgccgtagt acttttttgc gcagttatat gtatgatttc attcagcgtt ttgcaccgca
    24421 tttaacgcgt gatgtcgttg atgcggctgt cgcattgcgc tccaatgaag aaattgaggt
    24481 catgtttaaa gatataaaac tgccggaaaa ataatttctt gcgttatttt cggcaccttt
    24541 tatgtagcga aggtgccgga atatattctc ttttgttact tatttctata ccgtgcacaa
    24601 tatttttatc cctcatattt atagggtaaa ttacctgtaa agtagtgtct aattacccgc
    24661 ctcgcaaatt ccagttttca aatatctatt tcaggtcaaa agattgaatt attcactgga
    24721 gacgattcgt aaattcgctg gatttttgac taaagtttct ttaggatttg tctcatcgta
    24781 tgattaatta caccaatcat tcaacgtctt gctgataagt gatggcgatt atatgaggtt
    24841 agcaatgcct tctggaaatc aggaaccccg ccgtgatcct gaattaaaac gtaaagcctg
    24901 gctgacggtt tttcttggtt ctgcactttt ctgggtggtt gtcgcactgc tgatttggaa
    24961 agtgtgggga taactatggt cggtcaggag caactggagt cgtcaccatt atgccagcat
    25021 agtgacaatg aaccagaaac gaaacgggaa tgttccgtcg ttattccaga cgactggcaa
    25081 ctaacatcgc agcagcaagc ctttatagaa ctgtttgctg aagatgatca gccgaaacaa
    25141 taattatcat cattcttatt acccattttt aatgaattaa agggctttta atacaccgca
    25201 gcaataacag cttgagttat ctcaacacaa aataataacc gttaagggtg tagcctatga
    25261 tcaacacaaa tatgaaatat tggtcctgga tgggcgcgtt ttctctgtcg atgctcttct
    25321 gggccgaact cctctggatc attactcact gatccttgac cccgctgcgg cggggttgtc
    25381 atttgctttg ccacaaggtt tctcctcttt tatcaatttg ggttgttatc aaatcgttac
    25441 gcgatgtttg tgttatcttt aatatttacc ctgaagagaa tcagggcttc gcaaccctgt
    25501 cattaaggag gagctatgtc gtcaacccta cgagaagcca gtaaggacac gttgcaggcc
    25561 aaagataaaa cttaccacta ctacagcctg ccgcttgctg ctaaatcact gggcgatatc
    25621 acccgtctac ccaagtcact caaagttttg ctcgaaaacc tgctgcgctg gcaggatggt
    25681 aactcagtta ccgaagagga tatccacgca ctggcgggat ggctgaaaaa tgcccatgct
    25741 gaccgtgaaa ttgcctaccg cccggcaagg gtgctgatgc aggactttac cggcgtacct
    25801 gccgttgttg atctggcggc aatgcgcgaa gcggttaaac gcctcggtgg cgatactgca
    25861 aaggttaacc cgctctcacc ggtcgacctg gtcattgacc actcggtgac cgtcgatcgt
    25921 tttggtgatg atgaggcatt tgaagaaaac gtacgcctgg aaatggagcg caaccacgaa
    25981 cgttatgtgt tcctgaaatg gggaaagcaa gcgttcagtc ggtttagcgt cgtgccgcca
    26041 ggcacaggca tttgccatca ggttaacctc gaatatctcg gcaaagcagt gtggagcgaa
    26101 ttgcaggacg gtgaatggat tgcttatccg gatacactcg ttggtactga ctcgcacacc
    26161 actatgatca acggccttgg cgtgctgggg tggggcgtgg gtgggatcga agcagaagcc
    26221 gccatgttag gccagccggt ttccatgctt atcccggacg tagtgggctt taaacttacc
    26281 ggaaaattac gtgaaggtat taccgccaca gacctggttc tcactgttac ccaaatgctg
    26341 cgcaaacatg gcgtggtggg gaaattcgtc gaattttatg gtgatggtct ggattcacta
    26401 ccgttggcgg atcgcgccac cattgccaat atgtcgccag aatatggtgc cacctgtggc
    26461 ttcttcccaa tcgatgcagt aaccctcgat tacatgcgtt taagcgggcg cagcgaagat
    26521 caggtcgagt tggtcgaaaa atatgccaaa gcgcagggca tgtggcgtaa cccgggcgat
    26581 gaaccgattt ttaccagtat gttagaactg gatatgaatg acgttgaagc gagcctggca
    26641 gggccaaaac gcccacagga tcgcgttgca ctgcccgatg taccaaaagc atttgccgcc
    26701 agtaacgaac tggaagtgaa tgccacgcat aaagatcgcc tgccagtcga ttatgttatg
    26761 aacggacatc agtatcagtt acctgatggc gctgtggtca ttgctgcgat aacctcgtgc
    26821 accaacacct ctaacccaag tgtgctgatg gccgcaggct tgctggcgaa aaaagccgta
    26881 actcttggcc tcaagcggca accgtgggtc aaagcgtcgc tggcaccggg ttcgaaagtc
    26941 gtttctgatt atctggcaaa agcgaaactg acaccgtatc tcgacgaact ggggtttaac
    27001 cttgtgggat acggttgtac cacctgtatt ggtaactctg ggccgctgcc cgatcctatc
    27061 gaaacggcaa tcaaaaaagg cgatttaacc gtcggtgcgg tgctgtccgg caaccgtaac
    27121 tttgaaggcc gtatccatcc gctggttaaa actaactggc tggcctcgcc gccgctggtg
    27181 gttgcctatg cgctggcggg aaatatgaat atcaacctgg cttctgagcc tatcggccat
    27241 gatcgcaaag gcgatccggt ttatctgaaa gatatctggc catcggcaca agaaattgcc
    27301 cgtgcggtag aacaagtctc cacagaaatg ttccgcaaag agtacgcaga agtttttgaa
    27361 ggcacagcag agtggaagga aattaacgtc acacgatccg atacctacgg ttggcaggag
    27421 gactcaacct atattcgctt atcgcctttc tttgatgaaa tgcaggcaac accagcacca
    27481 gtggaagata ttcacggtgc gcggatcctc gcaatgctgg gggattcagt caccactgac
    27541 catatctctc cggcgggcag tattaagccc gacagcccag cgggtcgata tctacaaggt
    27601 cggggtgttg agcgaaaaga ctttaactcc tacggttcgc ggcgtggtaa ccatgaagtg
    27661 atgatgcgcg gcaccttcgc caatattcgc atccgtaatg aaatggtgcc tggcgttgaa
    27721 ggggggatga cgcggcattt acctgacagc gacgtagtct ctatttatga tgctgcgatg
    27781 cgctataagc aggagcaaac gccgctggcg gtgattgccg ggaaagagta tggatcaggc
    27841 tcaagccgtg actgggcggc aaaaggacct cgtctgcttg gaattcgtgt ggtgattgcc
    27901 gaatcgtttg aacgaattca ccgttcgaat ttaattggca tgggcatcct gccgctggaa
    27961 tttccgcaag gcgtgacgcg taaaacgtta gggctaaccg gggaagagaa gattgatatt
    28021 ggcgatctgc aaaatctaca acctggcgcg acggttccgg tgacgcttac gcgcgcggat
    28081 ggtagccagg aagtcgtacc ctgccgttgt cgtatcgaca ccgcgacgga gttgacctac
    28141 taccagaacg acggcatttt gcattatgtc atccgtaata tgttgaagta acaagtattt
    28201 gcttgccggt tattttgctt ccggcaagca aatgaattac acaatgcaag agggttattt
    28261 gttcagcaaa tggcccattt tctcggcttt ggtatcgaga taatgttcgt tattggggtt
    28321 acgacctaca atcaatggta cgcgttcaac aatattaatc cctgcttcgg tcagaatttc
    28381 gacttttttc gggttattgg ttaacaagcg gacttcattg acgccaagga gtttgaacat
    28441 atcagcgcaa agagtgaagt cgcgctcatc agcggcgaag cctaactggt ggttagcctc
    28501 tacggtatcg taaccttgat cctgcagtgc gtaagcgcgg attttattca gcagaccaat
    28561 gttacgacct tcctgacggt gatacagcaa aataccacgg ccttcctcgg caatttgcgt
    28621 caatgccgct tcgagctgga agccacaatc gcagcgcaag ctgaacaggg cgtcaccggt
    28681 cagacattcg gaatggacgc gcgcaagtac cggggtatgc ccggaaatat cggcatagac
    28741 tagcgcgaca tgatcgtgtc cggttgccag ttcttcaaat cccaccatca ggaaatcgcc
    28801 ccatggggtt ggcagtttgg cttctgccac acgtttaagc tgcatgaaat tctccagata
    28861 atgctggttc tgtattggct tattttgcca taacgagaag gggttcacct aatcacgacg
    28921 cgtcgatcgt tcacggaatg gcacaaatct gtcaattttt cttggaactg gcattttcag
    28981 ttatgattgt gggacttatc aaaaaggaga ggccatgcgt tcgattgccc gacgtaccgc
    29041 agtgggagct gcactattgt ttgtcatgcc agtagccgta tggatttctg gctggcgttg
    29101 gcaacctgga gaacaaagtt ggcgactaaa agcggctttt tgggttactg aaactgtcac
    29161 ccagccctgg ggcgtcatta cacatttgat tttgttcggc tggtttctct ggtgtctgcg
    29221 ttttcgcatt aaggctgcct ttgtattatt ttccattctg gcggcggcaa tccttgttgg
    29281 acaaggcgtt aaatcctgga tcaaagacaa agtacaggaa ccacgacctt ttgttatctg
    29341 gttggaaaaa acacatcata ttccggttga tgagttctac actttaaagc gagcagaacg
    29401 cggaaatcta gtaaaagaac agttggctga agagaaaaat attccacaat atttgcgttc
    29461 acactggcag aaagagacgg ggtttgcctt tccttccggt cacacgatgt ttgctgccag
    29521 ttgggcactg ctggccgttg gtttgctgtg gccgcgtcgg cgaacgttaa ccattgctat
    29581 cttgttggtc tgggcaacgg gagtcatggg aagccgcctg ctactcggga tgcattggcc
    29641 acgcgatctg gtggtagcta cgttgatttc gtggacgctg gtggcggtgg caacgtggct
    29701 tgcgcaacga atttgtgggc cattaacacc acctgcggaa gaaaatcgcg aaattgcaca
    29761 acgagaacaa gaaagttaac gctggttgat tttccgaatt taggccttaa atcatcaaca
    29821 atgcgcgtgg atgccatttc gcagacggcg cgaaaatggt actttaaagg gctattgcgg
    29881 taagttgacc ataatttatt cgctctaacc acataacggg aagtaatgtg aaatatttac
    29941 tcattttctt actggtgtta gcgatcttcg tgatttcggt cacgttgggt gcgcagaacg
    30001 atcaacaggt gacgtttaat tatctgttag cgcaagggga gtaccgtatt tccacattgc
    30061 tggcggtatt gtttgctgcg gggtttgcta tcggttggtt gatttgtggc ctgttctggc
    30121 tgcgagttcg tgtttccctg gcgcgcgctg aacgtaaaat aaagcgactg gaaaaccagc
    30181 tttcacccgc gactgacgtg gctgtagtgc cgcactcgtc agcggcgaag gaataacttt
    30241 ctatgctgga gttgttgttt ctgcttttgc ctgtagccgc tgcctatggc tggtatatgg
    30301 gccgcagaag tgcgcaacaa aacaagcaag atgaagccaa ccgcttgtcg cgtgattacg
    30361 tagcgggggt taacttcctg cttagtaatc aacaggataa agcggtagac ctgtttctcg
    30421 atatgcttaa agaggataca ggcaccgttg aagcccacct tacgctcgga aacctgttcc
    30481 gttcgcgtgg cgaagttgat cgcgctattc gcatccatca gaccctaatg gaaagcgcct
    30541 cgctgaccta tgaacagcgt ctgttggcga ttcaacaact ggggcgtgat tacatggccg
    30601 ccgggttata cgaccgcgcg gaagacatgt tcaatcagct gaccgatgaa actgacttcc
    30661 gcattggcgc gctgcaacag ttgctacaaa tctaccaggc taccagtgag tggcagaaag
    30721 caattgatgt tgccgaacgc ctggtgaagc tgggtaaaga taaacagcgc gtcgaaattg
    30781 cccatttcta ctgtgagtta gccctgcagc atatggccag cgacgatctc gatcgtgcca
    30841 tgaccttgct aaaaaaaggg gcggcggcag ataaaaacag cgcccgcgta tccatcatga
    30901 tgggacgcgt gtttatggcg aaaggagaat acgccaaagc cgtcgaaagt ttgcaacgcg
    30961 tcatatccca ggacagagaa ctggtcagcg aaacgctgga aatgttgcaa acctgctatc
    31021 agcagttggg taaaactgcc gaatgggcag aattcctgca acgcgcggtg gaagagaaca
    31081 ccggtgccga tgctgaattg atgcttgctg atatcatcga agcgcgcgac ggtagtgagg
    31141 ctgcacaggt ctatattaca cgccagcttc agcgtcatcc gaccatgcgt gtattccata
    31201 agctaatgga ttaccactta aatgaagcgg aagaagggcg cgccaaagag agtctgatgg
    31261 tgctgcgtga catggttggc gagaaggtgc gtagtaagcc tcgttatagc tgccagaaat
    31321 gtggttttac cgcatacacc ctctactggc attgtccgtc ttgtcgtgcc tggtcaacta
    31381 ttaaaccgat tcgcggtctt gatggcctgt aatttttaaa aaaatccgac tttagttaca
    31441 acatactagt tattgatgtc ccattgtgct ccggcaacga cggagcagaa aaagcctgct
    31501 aggggaacaa tcgcaactgt taatttttta tttccacggg tagaatgctc gccgtttacc
    31561 tgtttcgcgc cacttccggt gcccatcatc aagaaggtct ggtcatgacg ttaactgctt
    31621 catcttcttc ccgcgctgtt acgaattctc ctgtggttgt tgcccttgat tatcataatt
    31681 gtgatgacgc gctgtccttt gtcgacaaga tcgacccacg cgattgtcgt ctgaaggtcg
    31741 gtaaagagat gtttacattg tttgggccac agtttgtgcg cgaacttcaa cagcgtggtt
    31801 ttgatatctt tcttgacctg aaattccacg atatccccaa cactgcagcg cacgctgtcg
    31861 ctgctgcagc tgacttaggc gtgtggatgg tgaatgctca tgcctccggt ggggcgcgta
    31921 tgatgaccgc agcgcgtgag gcactggttc cgtttggcaa agatgcaccg cttttgattg
    31981 ctgtgacagt gttgaccagc atggaagcca gcgacctggc cgatcttggc gtgacactgt
    32041 cacctgcaga ttatgcagaa cgtctggcag cactgacgca aaaatgtggc cttgatggtg
    32101 tggtgtgttc tgctcaggaa gctgtgcgct ttaaacaggt attcggtcag gagttcaaac
    32161 tggttacacc gggcatccgt ccgcagggga gtgaagctgg tgaccagcgc cgcattatga
    32221 cgccagaaca ggcgttggcg gctggtgttg attatatggt gattggtcgc ccggtaacgc
    32281 aatcggtaga tccagcgcag acactgaaag cgatcaacgc ctctttacag cggagtgcat
    32341 gatgagtgat tccaacagcc gtctggtcta ctcaacggaa accgggcgta ttgatgagcc
    32401 caaagcggcc ccagtacgcc ctaaaggcga tggagcggtg cgtattcagc gtcagaccag
    32461 tggacgtaaa ggtaagggcg tttgcctgat taccggtatc gatctcgatg atgccgaact
    32521 gacaaaactt gcagcggaac tgaagaaaaa atgcggctgc ggcggagcag ttaaagatgg
    32581 agttattgaa atccagggcg ataagcgtga tttattaaaa tcactacttg aagcgaaagg
    32641 gatgaaggta aaactcgcag gcggttaaca taaaaagcca cggatatatc cgtggctttc
    32701 gaatatttta ctgtgtgtat tattcagtta tttcgtactg aacagaatca agcgtattat
    32761 aattgcgagt cgataatcga catttattta cgattattta ccgacctggt gactaataac
    32821 accaccgaca gctgcaccac ctaatgtacc caacgtactg ccatcggtca gtactgcacc
    32881 gcctaatgcc cctgcacccg cgccgattgc ggtgttgcgg tcccgtttag accagttaga
    32941 acaggcactc agagacattg ccaaagtaat tgccagaaca gccgcggtca tttttttgct
    33001 cgttacaaac ataatactct ctcctgaatt tatgattcac ggaagtaagc tctctataac
    33061 tataatagct aagaataagc ctggtgaaat tattccgtga aatctgctcg cgcgcagggt
    33121 tatatcacg
//
LOCUS       DAJVQH010000030        33113 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJVQH010000030 DAJVQH010000000
VERSION     DAJVQH010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.030"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            60..1337
                     /gene="waaA"
                     /locus_tag="OQM87_001535"
     CDS             60..1337
                     /gene="waaA"
                     /locus_tag="OQM87_001535"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCS1507714.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1345..1824
                     /gene="coaD"
                     /locus_tag="OQM87_001536"
     CDS             1345..1824
                     /gene="coaD"
                     /locus_tag="OQM87_001536"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCS1507715.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1863..2672)
                     /gene="mutM"
                     /locus_tag="OQM87_001537"
     CDS             complement(1863..2672)
                     /gene="mutM"
                     /locus_tag="OQM87_001537"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCS1507716.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2770..2937)
                     /gene="rpmG"
                     /locus_tag="OQM87_001538"
     CDS             complement(2770..2937)
                     /gene="rpmG"
                     /locus_tag="OQM87_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCS1507717.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2958..3194)
                     /gene="rpmB"
                     /locus_tag="OQM87_001539"
     CDS             complement(2958..3194)
                     /gene="rpmB"
                     /locus_tag="OQM87_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCS1507718.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3411..4079)
                     /gene="radC"
                     /locus_tag="OQM87_001540"
                     /pseudo
     CDS             complement(3411..4079)
                     /gene="radC"
                     /locus_tag="OQM87_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            4251..5471
                     /gene="coaBC"
                     /locus_tag="OQM87_001541"
     CDS             4251..5471
                     /gene="coaBC"
                     /locus_tag="OQM87_001541"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCS1507719.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5449..5907
                     /gene="dut"
                     /locus_tag="OQM87_001542"
     CDS             5449..5907
                     /gene="dut"
                     /locus_tag="OQM87_001542"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCS1507720.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6014..6610
                     /gene="slmA"
                     /locus_tag="OQM87_001543"
     CDS             6014..6610
                     /gene="slmA"
                     /locus_tag="OQM87_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCS1507721.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6647..7288)
                     /gene="pyrE"
                     /locus_tag="OQM87_001544"
     CDS             complement(6647..7288)
                     /gene="pyrE"
                     /locus_tag="OQM87_001544"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCS1507722.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7354..8070)
                     /gene="rph"
                     /locus_tag="OQM87_001545"
     CDS             complement(7354..8070)
                     /gene="rph"
                     /locus_tag="OQM87_001545"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCS1507723.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8197..9060
                     /locus_tag="OQM87_001546"
     CDS             8197..9060
                     /locus_tag="OQM87_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCS1507724.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9281..10105
                     /gene="dinD"
                     /locus_tag="OQM87_001547"
     CDS             9281..10105
                     /gene="dinD"
                     /locus_tag="OQM87_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCS1507725.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10396..11013
                     /locus_tag="OQM87_001548"
     CDS             10396..11013
                     /locus_tag="OQM87_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCS1507726.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11010..12527)
                     /gene="ligB"
                     /locus_tag="OQM87_001549"
     CDS             complement(11010..12527)
                     /gene="ligB"
                     /locus_tag="OQM87_001549"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCS1507727.1"
                     /translation="MEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTG
                     VRKMADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQK
                     VSLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTL
                     NSLAVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLP
                     FVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVA
                     SLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLGGQGIPRIDDVVWRGAERTK
                     PTPPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEH
                     IFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASD
                     ERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(12549..12806)
                     /locus_tag="OQM87_001550"
     CDS             complement(12549..12806)
                     /locus_tag="OQM87_001550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507728.1"
                     /translation="MIYCNKDDSQEASSQTPFLPLGFAFTTLYICQPDYSGGEDESMD
                     GDINKYLVLAIICVGGLSGLVASQSTGRNFPPATANKTVGR"
     gene            12946..13569
                     /gene="gmk"
                     /locus_tag="OQM87_001551"
     CDS             12946..13569
                     /gene="gmk"
                     /locus_tag="OQM87_001551"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCS1507729.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13624..13899
                     /gene="rpoZ"
                     /locus_tag="OQM87_001552"
     CDS             13624..13899
                     /gene="rpoZ"
                     /locus_tag="OQM87_001552"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCS1507730.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13918..16026
                     /gene="spoT"
                     /locus_tag="OQM87_001553"
     CDS             13918..16026
                     /gene="spoT"
                     /locus_tag="OQM87_001553"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCS1507731.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16033..16722
                     /gene="trmH"
                     /locus_tag="OQM87_001554"
     CDS             16033..16722
                     /gene="trmH"
                     /locus_tag="OQM87_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCS1507732.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16728..18809
                     /gene="recG"
                     /locus_tag="OQM87_001555"
     CDS             16728..18809
                     /gene="recG"
                     /locus_tag="OQM87_001555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCS1507733.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18843..20048)
                     /gene="gltS"
                     /locus_tag="OQM87_001556"
     CDS             complement(18843..20048)
                     /gene="gltS"
                     /locus_tag="OQM87_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCS1507734.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20328..21719
                     /gene="xanP"
                     /locus_tag="OQM87_001557"
     CDS             20328..21719
                     /gene="xanP"
                     /locus_tag="OQM87_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCS1507735.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21840..23549
                     /locus_tag="OQM87_001558"
     CDS             21840..23549
                     /locus_tag="OQM87_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1507736.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23602..25920)
                     /gene="yicI"
                     /locus_tag="OQM87_001559"
     CDS             complement(23602..25920)
                     /gene="yicI"
                     /locus_tag="OQM87_001559"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS1507737.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25930..27313)
                     /locus_tag="OQM87_001560"
                     /pseudo
     CDS             complement(25930..27313)
                     /locus_tag="OQM87_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27606..27700
                     /locus_tag="OQM87_001561"
     tRNA            27606..27700
                     /locus_tag="OQM87_001561"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27640..27642,aa:Sec,seq:tca)
     gene            28000..29184
                     /locus_tag="OQM87_001562"
     CDS             28000..29184
                     /locus_tag="OQM87_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1507738.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSALIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            29596..30639
                     /locus_tag="OQM87_001563"
     CDS             29596..30639
                     /locus_tag="OQM87_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCS1507739.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSRIQYQ"
     gene            31154..31582
                     /locus_tag="OQM87_001564"
                     /pseudo
     CDS             31154..31582
                     /locus_tag="OQM87_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31612..32763)
                     /locus_tag="OQM87_001565"
                     /pseudo
     CDS             complement(31612..32763)
                     /locus_tag="OQM87_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         8274 a         8722 c         8197 g         7920 t
ORIGIN      
        1 ggtaaagcca cagctaaata catagaatcc ccagcacatc cataagtcag ctatttacta
       61 tgctcgaatt gctttacacc gcccttctct accttattca gccgctgatc tggatacggc
      121 tctgggtgcg cggacgtaag gctccggcct atcgaaaacg ctggggtgaa cgttacggtt
      181 tttaccgcca tccgctaaaa ccaggcggca ttatgctgca ctccgtctcc gtcggtgaaa
      241 ctctggcggc gatcccgttg gtgcgcgcgc tgcgtcatcg ttatcctgat ttaccgatta
      301 ccgttacaac catgacgcca accggttcgg agcgcgtaca atcggctttc gggaaggatg
      361 ttcagcacgt ttatctgccg tatgacctgc ccgatgcgct caaccgtttc ctgaataaag
      421 tcgaccccaa actggtgttg attatggaaa ccgaactatg gcctaacctg attgcggcgc
      481 tacataaacg taaaattccg ctggtgatcg ctaacgcgcg actctctgcc cgctcggccg
      541 caggttatgc caaactgggt aaattcgtcc gccgcttgct gcgtcgtatt acgctgattg
      601 ctgcgcaaaa tgaagaagat ggtgcacgtt ttgtggcgct gggcgcaaaa aataaccagg
      661 taaccgttac cggtagcctg aaattcgata tttctgtaac tccgcagttg gctgctaaag
      721 ccgtgacgct acgccgccaa tgggcaccac accgcccggt atggattgcc accagcactc
      781 acgaaggtga agagagcgtg gtcattgccg cacatcaggc attgttacag caattcccga
      841 atttattgct catcctggta ccccgtcatc cagaacgctt cccggatgcg attaaccttg
      901 tccgccaggc tggactaagc tatatcacac gctcttcagg ggaagtcccc tccaccagca
      961 cgcaggttgt ggttggcgat acgatgggcg agttgatgtt actgtacggc attgccgatc
     1021 tcgcctttgt tggcggttca ctggttgaac gtggtgggca taatccgctg gaagctgccg
     1081 cacacgctat tccggtattg atggggccgc atacttttaa ctttaaagac atttgcgcgc
     1141 ggctggagca ggcaagcggg cttattaccg ttaccgatgc cactacgctt gcaaaagagg
     1201 tttcctcttt actcaccgac gccgtttacc gtagtttcta tggccgtcat gccgttgaag
     1261 tactgtatca aaaccagggc gcgctacagc gtctgcttca actgctggaa ccttacctgc
     1321 caccgaaaac gcattgaggt tgttatgcaa aaacgggcga tttatccggg tactttcgat
     1381 cccattacca atggtcatat cgatatcgtg acgcgcgcca cgcagatgtt cgatcacgtt
     1441 attctggcga ttgccgccag ccccagtaaa aaaccgatgt ttaccctgga agagcgtgtg
     1501 gcactggcac agcaggcaac cgcgcatctg gggaacgtgg aagtggtcgg gtttagtgat
     1561 ttgatggcga acttcgcccg taatcaacac gctacggtgc tgattcgtgg cctgcgtgcg
     1621 gtggcggatt ttgaatatga aatgcagctg gcgcatatga atcgccactt aatgccagaa
     1681 ctggaaagtg tgtttctgat gccgtcgaaa gagtggtcgt ttatctcttc atcgttggtg
     1741 aaagaggtgg cgcgccatca gggcgatgtc acccatttcc tgccggagaa tgtccatcag
     1801 gcgctgatgg caaaattagc gtagcgttta tgccggatgg tatgccatcc ggctcgcatg
     1861 aattacttct ggcactgccg acaataaaac gttgcccgct gcgcatgttt agtcgccaca
     1921 atcggcgtac cgcacacccg acacggctca ccttttcgcc cgtaaacctg caattcctgc
     1981 gcgaaatagc ccggtttacc atcactttgc agaaaatctt tcagcgttgt accaccctgc
     2041 tcaatcgaac gcagcaacac cgctttaatc acccgagcta acaattcaca ctctgccagc
     2101 gacagtgatg acgccagccg atccggatgg atccccgccg caaacagtga ttcgctggca
     2161 tagatattcc ctacccctac caccagcttg ttatccatca gccacggttt aatcgccgtt
     2221 tttttcttcg cgcacttctg atgcagatac tcaccattga aatcgtcgct aagcggctcc
     2281 ggtccaagat gggccagcac attatgccct tccagctctt tggtccacag ccaggcacca
     2341 aagcggcgcg gatcggtgta gcgcagcact ttaccgttgc tcatcaccaa atccacatgg
     2401 tcatgctttt cagggggaag ttcttctgga aggatgcgca ggctgccaga catccccaaa
     2461 tgaatgataa tccagccctc aggcagctcc agcagcagat atttagcccg ccgctgcacg
     2521 ctaagcactg gttgatcgct taatcggtag atctcttctg aaaccggcca gcgcaagcgt
     2581 ccgttgcgca ccaccgcatg aagaatggtt gcaccaacga gattcggttc tatgccgcga
     2641 cggctggttt caacttcggg taattcaggc atagcatctc caggaatgaa cagatgcaat
     2701 caatatgggg gcaagcagat aacaaaaaac cccgccggag cgaggttttt cgttacatca
     2761 aagcgagaat tatttgattt tcgcttcttt gtagatcacg tgctgacgaa caactggatc
     2821 gaattttttc agttccagtt tttccggctt agtacgtttg ttcttcgtag tggtatagaa
     2881 gtgaccagta ccagcagaag aaaccagctt gattttctca cgaatacctt tagccatgat
     2941 ttatttcctc taagtactta gtacttttcg ccacgggcac gcagttcagc cagaactgta
     3001 tcgatgcctt ttttatcgat tacacgcata cctttagcag atacacgcag ggtgacaaaa
     3061 cgcttctcgc tctcaaccca gaaacggtga gagtgcaggt tcggcaggaa acggcgttta
     3121 gtcgcgttca gtgcgtggga acggttgtta ccggtcaccg gacgcttgcc agtaacttgg
     3181 cagactcggg acatgtctat tctccaaaaa tcaaattagc tcgagcttcg tatggggtat
     3241 tggcgcctcg tcaggcttta cagcccggtc atcgcagttc tatgtgaact ctcgattgcc
     3301 aggcccaaat gccaaacccg agattctcaa aggtggcgta gtatacgctg actcagcgat
     3361 gtgctcaagt cccgaacaga caaagatccc gaaggatcgc gcatagcggg ttaaatccag
     3421 ccgcgttcgg caaaagaaac atactctcca cgcccaatca cgatatggtc gagcacgcgt
     3481 aaatccatga actgacaact ctttattatc cgttcagtaa tgagtttatc cgctttactg
     3541 ggttcagcac aacccgaagg gtgattatgt gcaaggatca gcgccgaggc gtttattttt
     3601 atcgcttagc ggataatttc ccgaggatgg acttcaacat ggtttagcgt gccggaaaaa
     3661 agacggctat gcgttataac ccggtgttgt gagtcgagaa agatcaccat aaagatctcc
     3721 cgctcctcac ccgtgagctg gctttgtaaa aattcccgcg tcatctccgg gctgagtaaa
     3781 gggctttctt cacgcatccg cacgttgtag taacgtcgcg ccagttcagc aatccccttt
     3841 aactgggcaa atttcgctac gccaattcca tgaacaccac taaattgttc atattcagag
     3901 gttaacaagc cataaagaga gccgaaattc tccagcatct cttttgccag ggttaataca
     3961 tctttaccgc gcgttccggt acgcagaaat agcgccagca gctcgacatc cgttaaggcg
     4021 ctaataccaa acttcagcat tttttcgcgc ggcatcaaca gctgtgcatt gtttttcacc
     4081 ttcacctcct ttgtggtgct cgcatcctgt catagccgtt ccgttaaatc gacggccagt
     4141 tttcaatcct ggaaagagcc tcgcaaagtg aatctcaggt gaaggcacga caacaaaagt
     4201 attgtgataa aatcgccaac ttctggtgtc acccaacagg aaaaatcatc atgagcctgg
     4261 ccggtaaaaa aatcgttctc ggcgttagcg gcggtattgc tgcctataaa acccctgaac
     4321 tggtgcgtcg tttgcgcgat cgcggggccg acgtccgcgt agccatgacc gaagcggcaa
     4381 aagcctttat caccccactt agcttgcagg cggtttctgg ttatcccgtt tccgacagtc
     4441 tgcttgaccc ggcagccgaa gcagctatgg gccatattga gctgggtaaa tgggctgatt
     4501 tagtgattct cgcccctgcc acggcagatt tgattgcccg tgttgctgcc ggaatggcga
     4561 atgacctggt atcgacgatt tgtctggcta cacctgcgcc tgtagccgtg ctccccgcca
     4621 tgaaccagca gatgtaccgt gccgctgcca cgcagcataa tttagaggtg cttgcttccc
     4681 gtggtttgct catctggggg ccagacagtg gcagtcaggc ttgtggtgat atcggtcctg
     4741 gacgaatgct cgatccgtta accattgtgg atatggcggt ggcgcatttt tcgcccgtca
     4801 acgacctgaa acatctgaac attatgatta ccgccggtcc gacgcgtgaa ccgctcgatc
     4861 cggtgcgtta tatctctaat cacagctccg gcaagatggg ttttgctatc gccgccgctg
     4921 ctgcccgtcg tggcgcgaac gtcacgctgg tatcaggtcc agtttcacta ccgacgccac
     4981 cgtttgttaa acgtgttgat gtgatgaccg cgctggaaat ggaagccgcc gtgaatgctt
     5041 ctgtacagca gcaaaatatt tttatcggtt gcgccgccgt ggcggattat cgcgcagcta
     5101 ccgtggcccc agaaaaaatc aaaaagcagg ccacgcaggg tgatgaatta acaataaaaa
     5161 tggttaaaaa ccccgatatc gtcgcaggcg ttgccgcact aaaagaccat cgaccctacg
     5221 tcgttggatt tgccgccgaa acaaataatg tggaagaata cgcccggcaa aaacgtatcc
     5281 gtaaaaacct tgatctgatc tgcgcgaacg atgtttccca gccaactcaa ggatttaaca
     5341 gcgacaacaa cgcattacac cttttctggc aggacggaga taaagtctta ccgcttgagc
     5401 gcaaagagct ccttggccaa ttattactcg acgagatcgt gacccgttat gatgaaaaaa
     5461 atcgacgtta agattctgga cccgcgcgtt gggaaggaat ttccgctccc gacttatgcc
     5521 acctctggct ctgccggact tgacctgcgt gcctgtctcg acgacgccgt agaactggct
     5581 ccgggtgaca ctacgctggt tccgaccggg ctggcgattc atattgccga tccttcactg
     5641 gcggcaatga tgctgccgcg ctccggattg ggacataagc acggtatcgt gcttggtaac
     5701 ctggtaggat tgatcgattc cgactatcag ggccagttga tgatttccgt gtggaaccgt
     5761 ggtcaggaca gcttcaccat tcaacctggc gaacgcatcg cccagatgat ttttgttccg
     5821 gtagtacagg ctgaatttaa tctggtggaa gatttcgacg ccaccgaccg cggtgaaggc
     5881 ggctttggtc actctggtcg tcagtaacac atacgcatcc gaataacgtc ataacatagc
     5941 cgcaaacatt tcgtttgcgg tcatagcgtg ggtgccgcct ggcaagtgct tattttcagg
     6001 ggtattttgt aacatggcag aaaaacaaac tgcgaaaagg aaccgtcgcg aggaaatact
     6061 tcagtctctg gcgctgatgc tggaatccag cgatggaagc caacgtatca cgacggcaaa
     6121 actggccgcc tctgtcggcg tttccgaagc ggcactgtat cgccacttcc ccagtaagac
     6181 ccgcatgttc gatagcctga ttgagtttat cgaagatagc ctgattactc gcatcaacct
     6241 gattctgaaa gatgagaaag acaccacagc gcgcctgcgt ctgattgtgt tgctgcttct
     6301 cggttttggt gagcgtaatc ctggcctgac ccgcatcctc actggtcatg cgctaatgtt
     6361 tgaacaggat cgcctgcaag ggcgcatcaa ccagctgttc gagcgtattg aagcgcagct
     6421 gcgccaggta ttgcgtgaaa agagaatgcg tgagggtgaa ggttacacca ccgatgaaac
     6481 cctgctggca agccagatcc tggccttctg tgaaggtatg ctgtcacgtt ttgtccgcag
     6541 cgaatttaaa taccgcccga cggatgattt tgacgcccgc tggccgctaa ttgccgcgca
     6601 gttgcagtaa tatgacgccg gatgactttt catccggcga gtttctttaa acgccaaact
     6661 cttcgcgata ggccttaacc gccgccagat gttccgccat ttccggcttc tcttccagat
     6721 aggcaatcag atctttcagg gtgatgatag agatcacttt gcagttgtaa tcacgctcaa
     6781 cttcctgaat cgccgaaatc tcgccgcgcc cgcgttcctg acgatcgagc gaaatcaaca
     6841 cgccagcaag cgtcgcgcca ttggcctgaa taatctccat cgactcgcga atcgccgttc
     6901 cggcggtgat cacatcatct accagcatta cgcgtccttg taacgcgcta ccaaccagat
     6961 tgccgccttc accgtggtct tttgcttctt tgcggttaaa gcagtacggc aggtccaggt
     7021 cgtgatgctc cgccagtgcc acagcggttg tggtggcaat cgggatccct ttgtaagcag
     7081 ggccaaacag cagatcgaac tcaatgccgg aatccaccaa cgcttcagcg taaaaacggc
     7141 ctaacagtgc cagatcgcgc ccggtattaa acagcccggc gttgaagaaa taggggcttt
     7201 tgcgcccgga tttcagcgta aactcgccaa actttaacac ctgcttgcta agcgcaaatt
     7261 caataaactg gcgctgatat ggtttcatgc cttcgctcct catcttactt ttctacagac
     7321 aaaaaaaagg cgactcatca gtcgccttaa aaatcagttt gccagcgccg ccttctgcgt
     7381 cgctacaatg gattcgattc cccctcgggc cagcgccaac aaggtgagta gctcttcatg
     7441 ggtgaacggc tcgccttctg ccgtcccctg cacttcaatg atgcgcccgt cttcggtcat
     7501 cactacgttc atgtcggtct ctgcggctga gtcttcaacg tattccagat cgcaaattgc
     7561 ttcaccgttt acaataccga cagaaactgc ggctaccatc cctttcatcg ggttggtttt
     7621 cagcttgccg ttttccacca gcttctgtag cgcatctgcc agcgccacgc aggcacccgt
     7681 aatcgacgcg gtacgagtgc caccatcagc ctgaagcacg tcgcagtcca gcgtaatggt
     7741 gaactcaccc agcgctttca aatctactgc cgcgcgaaga gcacgggcga tcagacgctg
     7801 gatttccatt gtgcgtccac cctgcttacc tttcgccgct tcacgagcgt tacgggtgtg
     7861 ggtagaacgt ggcagcatgc cgtactctgc ggtgatccag ccctggccct gacctttcag
     7921 gaagcgcggc acgccttctt caatagaggc ggtacacaat actttggtat cgccaaattc
     7981 gaccagcacc gagccttctg catgttttgt atagttacga gtcagggtaa cgggacgcac
     8041 ctgattattg ctacggcctg ctggacgcat attgaaatct ccggcttgaa acaaatgtgg
     8101 ctgcgcatta tacggacttc cggcggttat tcctatcctg acaaggcatc gatggctata
     8161 atccttccac ctctcctttt ataaacagga acgtctatga tccgcagtat gaccgcctac
     8221 gcccggcgtg aaatcaaggg tgaatggggg agcgcaacct gggaaatgcg ctcggtaaac
     8281 cagcgttatc tggaaactta ctttcgtctg ccggagcagt tccgtagcct tgaacctgtc
     8341 gttcgcgagc gtattcgttc tcgcctgacg cgcggtaaag tggaatgtac cctgcgctat
     8401 gagccagatg ttagcgcaca aggtgagctg atcctcaatg aaaaactggc taaacagctg
     8461 gtaactgccg cgaactgggt aaaaatgcag agtgacgaag gggaaatcaa cccggttgat
     8521 attctacgct ggccgggcgt gatggcagcc caggagcagg atctcgacgc gattgccgct
     8581 gaaattctcg cggcgttgga tggtacgctg gacgacttta tcgtcgcgcg cgaaaccgaa
     8641 ggtcaggcac tgaaagcatt gatcgagcag cgtctggaag gcgtcaccgc cgaagtggtc
     8701 aaagtccgcg cccatatgcc ggaaattctg caatggcagc gtgagcgtct ggtcgcgaag
     8761 ctggaagatg ctcaggttca gttggaaaac aaccgtctgg agcaggagtt ggttctgctg
     8821 gcacaacgaa ttgacgttgc cgaagagctg gaccgcctcg aagcgcatgt taaagagacg
     8881 tacaacattc tgaagaaaaa agaagcggtt ggtcgtcgtc tggactttat gatgcaggag
     8941 ttcaaccgtg agtcgaacac tcttgcgtcg aaatctatca atgccgaagt gacaaactcc
     9001 gccatcgagc tgaaagtgtt gattgagcag atgcgcgagc agattcagaa tattgagtaa
     9061 ctcgtaacac caattctgac ttttcagcct tcacaaatgc cgcccactca aacagagcgg
     9121 catttttctt ccccgcaact tcaattctgt tttgcgtgcc tgctccagat tttgcgatgt
     9181 ttttttgccc agcacactga gaacgtgaga tactcacaac tgtatataaa tacagttaca
     9241 gatttacttt ctttgcaatc gatatcacat ggagtgggca atgaacgaac atcatcaacc
     9301 ttttgaagag ataaaactga ttaatgcaaa cggagcagaa caatggtcag caagacaact
     9361 tgggaaacta ctgggttatt cagagtatcg tcactttata cctgtattaa cgcgcgccaa
     9421 agaggcctgt gagaacagtg gtcacacaat tgatgaccat ttcgaggaga tcctcgatat
     9481 ggtcaaaatt ggctcaaatg ccaaaagagc attaaaagac atcgtactct cccgctatgc
     9541 ctgttacctg gtagtacaaa acggcgaccc cgcgaaaccg gtcattgcgg cagggcagac
     9601 ttattttact atccagaccc gacggcagga gcttgctgac gatgaagcat ttaagcaact
     9661 tcgtgaagac gaaaagcgtc tgtttctgag aaatgagttg aaagaacata acaaacaatt
     9721 ggttgaggca gcacagcaag ctggggtagc gacagctact gatttcgcca tatttcagaa
     9781 tcatggttac caggggctgt atggtggatt agatcagaaa gccatccacc aactgaaggg
     9841 gctgaaaaag agtcagaaga tcctggatca tatgggttca acagaactgg cggctaatct
     9901 ctttcgagct acccaaacag aagaaaaact caagcgggat agcgtgaatt caaaacagca
     9961 agctaatacc acacactttg acgtgggtag caaggtgagg caaaccattc aggaacttgg
    10021 cggaaccatg cctgaggagt tgccgacccc gcaggtcagc atcaagcagt tggaaaacag
    10081 tgtaaaaatt acagagaaga aatagcagaa acgaaaaact ctttttgttg attgagacac
    10141 ccgatgcgta agccaaggtc caggtgcagt gaacacaatg gctaaacatt gcacctttct
    10201 tttcccccct cagttttaac ctattttttc ttatgcattt tctcagacaa gaagtcagaa
    10261 gaatgcatct acgctacaga aaatagcgat ttcacataac taaaagttat caatttcctc
    10321 tcaccttaaa aaatatcaat cgtgacaatg cgcacaaatc gctaccctgc cagacagatt
    10381 tttagggaga gaaccatgct gttacacatt ttgtatttgg ttggcattac tgccgaagcc
    10441 atgacggggg cgctggcggc cgggcgacgg cgcatggata catttggcgt aattattatt
    10501 gcaaccgcca ccgcaattgg cggagggtca gtgcgcgata ttctgctggg ccactatccg
    10561 ctcggctggg tcaaacaccc ggaatatgtg attatcgtcg ctaccgccgc agtactcacc
    10621 actatcgttg ctcctgtaat gccttacctg cgcaaagtgt ttctggtgct cgatgcgctc
    10681 ggactggtgg tcttttctat catcggcgca caggttgcac tggatatggg gcacggccca
    10741 attattgccg ttgtcgcggc ggtaaccacc ggtgtatttg gcggcgtttt gcgcgatatg
    10801 ttctgtaaac gcattccgct ggtctttcaa aaagagttgt atgccggtgt ctcatttgcc
    10861 tccgccgtgc tgtacatcgc gctgcaacac tatgtttcta accatgatgt ggtgattatc
    10921 tccaccctgg tattcggctt ttttgctcgt ttactggcgc tacgtcttaa gctgggatta
    10981 ccggtctttt actacagcca cgaaggtcac taaggttcaa aacctgtgat ctgctgggca
    11041 gccagccaac tgcccagctt cttgatttgc gcattttcct tccattcaat aacctgtctg
    11101 gcgcgcccag atccagtccc cggcagctgc tgccagaact gctccgtgct aaataaaagt
    11161 tgcgaccagg atcgttcatc actggcatta agcgccgccc gggttagcgg tattcccatt
    11221 gccatcaccc agcgagtaaa aggctggtta cgagccagat taaactgatg ccataactgc
    11281 gcacttttac ttttcgcgat ccccggcgtg ttctgtaatt gctctggcgt taataaaagc
    11341 caggaaaaga tatgctcaaa gcgatgagtc tgatgcagcg cgcgccaacc ggcctcacca
    11401 atgccatcca gcccgagaac ctgttttgac cccagccaga ctaagcgtga aatgaactgt
    11461 tcctgacaaa catcagaagc aaagtagcag gtcaacgagt taaagcggtt ttctggtggt
    11521 gtcggttttg tacgttctgc accgcgccag accacatcat caatgcgagg aataccctga
    11581 ccgccaaggc tgacgaggat ctgatcacca ggcgcaatat cccactcctg ccagcgcctg
    11641 acggaaccaa tattcacccg ctggactttt ttatcatcca gcatgacagg tgcgagtgac
    11701 gcaaccaccg atattttacc gctcttaccc accgcaaact gaatcgcctt cacttcggca
    11761 acctgagcta caggttgata tttccaggcc accagccact ccgcctggcc cggtagccaa
    11821 tgacgggatt cgggttcctt agcccctcgc acaaccacgc catcggtgac gaagggtaat
    11881 ttcgctttcc accactcatt gcgtacgcgc gcaacctcat cagcattttt aaccgcacgg
    11941 gtatacctct gcgttagagt aaaacctgcg gtagccagct cctttaaacg atcggtcatt
    12001 aactgcggtc catccggcca tgcccagaca aaaacagcca gtgagtttag cgtgtcgcta
    12061 ttcccctggc gcatcatcag gccagcaact tttgcgcggg catttattcc ccccatttgt
    12121 tgttggatat gcccctcgcg ctggagaaat atttccccct gaagcgtact attggctaaa
    12181 ggcccgctaa cggtttgcgg cacagcggaa attaagctaa ctttctgcgt ccagtcctcg
    12241 cctttcaggc cattaccgcg actgattgct ttgttcagtt tcccgtcccg ataaaccagg
    12301 gttaccgcaa cgccatcaac ttttggctgc acccaaagat cactacgttc tcgcatccac
    12361 agacttaatg cgtttttatc cgccattttg cgtacacccg tatgggcaac cggatgcata
    12421 accgcaccat ttaaaggcgg catcatcacg tcgcggggct cgctcacaaa gcagcgttgc
    12481 cactgcgtaa gacgggcgct taactgatcg taaacaccgt cttccacctc actttttcct
    12541 tccagtagtc atcgtcccac tgttttattt gctgttgcag gcgggaaatt tcttcctgtg
    12601 ctctggctgg cgaccaggcc ggacagaccg cccacacaga tgattgccag cacaagatac
    12661 ttattaatat cgccatccat actttcatct tcacctccgc tgtagtcagg ctggcagata
    12721 tacaacgtgg tgaaggcaaa gccgagtggc aaaaacggag tctgcgagga cgcttcctga
    12781 gaatcgtctt tattgcagta aatcacaggc aaatgcggaa acagctacgc aaaacgcaac
    12841 aactttgcgc aaaaagtgtg agcaagggct acgtcacatg gccgcgccgt gtataataag
    12901 ctcgtatgta ggctttattt cgctaatcac atacgaaaga tactcatggc tcaaggcacg
    12961 ctttatattg tttctgcccc cagtggcgcg ggtaaatcca gcctgattca ggctttatta
    13021 aaaacccaac cgttgtatga cacccaggtt tctgtttcac acaccacacg ccaaccgcgt
    13081 cctggtgaag tccacggtga acattatttc tttgttaatc atgatgaatt taaagaaatg
    13141 attagcagag atgcgttcct cgaacacgca gaagtttttg gtaattacta tggcacttcg
    13201 cgtgaggcca ttgagcaagt actggcgacc ggtgtcgatg tttttctcga tatcgactgg
    13261 cagggcgcgc agcaaattcg ccagaagatg ccgcacgcgc ggagtatctt tattttaccg
    13321 ccgtccaaaa ttgaactgga ccgccgtcta cgcggtcgcg gtcaggacag cgaagaggtc
    13381 attgcaaagc gtatggcgca agctgttgca gaaatgagcc attacgccga atatgattat
    13441 ctgattgtga atgatgactt cgataccgcg ttgaccgatt tgaagaccat tattcgcgcc
    13501 gaacgtctgc gcatgagccg ccaaaagcag cgtcatgacg ctttaatcag caaattgttg
    13561 gcagactgaa cctgatttca gtatcatgcc cagtcatttc ttcacctgtg gagcttttta
    13621 agtatggcac gcgtaactgt tcaggacgct gtagagaaaa ttggtaaccg ttttgacctg
    13681 gtactggtcg ccgcgcgtcg cgctcgtcag atgcaggtag gcggaaagga tccgctggta
    13741 ccggaagaaa acgataaaac cactgtaatc gcgctgcgcg aaatcgaaga aggtctgatc
    13801 aacaaccaga tcctcgacgt tcgcgaacgc caggaacagc aagagcagga agccgctgaa
    13861 ttacaagccg ttaccgctat tgctgaaggt cgtcgttaat cacaaagcgg gtcgcccttg
    13921 tatctgtttg aaagcctgaa tcaactgatt caaacctacc tgccggaaga ccaaatcaag
    13981 cgtctgcggc aggcgtatct cgttgcacgt gatgctcacg aggggcaaac acgttcaagc
    14041 ggtgaaccct atatcacgca cccggtagcg gttgcctgca ttctggccga gatgaaactc
    14101 gactatgaaa cgctgatggc ggcgctgctg catgacgtga ttgaagatac tcccgccacc
    14161 taccaggata tggaacagct ttttggtaaa agcgtcgccg agctggtaga gggggtgtcg
    14221 aaacttgata aactcaagtt ccgcgataag aaagaggcgc aggccgaaaa ctttcgcaag
    14281 atgattatgg cgatggtgca ggatatccgc gtcatcctca tcaaacttgc cgaccgtacc
    14341 cacaacatgc gcacgctggg ctcacttcgc ccggacaaac gtcgccgcat cgcccgtgaa
    14401 actctcgaaa tttacagccc gctggcgcac cgtttaggta tccaccacat taaaaccgaa
    14461 ctcgaagagc tgggttttga ggcgctgtat cccaaccgtt atcgcgtaat caaagaagtg
    14521 gtgaaagccg cgcgcggcaa ccgtaaagag atgatccaaa aaatcctctc ggaaatcgaa
    14581 gggcgtttgc aggaagcggg aattccctgc cgcgtcagtg gtcgcgagaa gcatctttat
    14641 tcgatttact gcaaaatggt gctcaaagag cagcgttttc actcaatcat ggacatctac
    14701 gctttccgcg tgatcgtcaa tgattccgac acctgttatc gcgtgctcgg gcaaatgcat
    14761 agcctgtaca agccgcgtcc gggtcgcgtg aaagactaca tcgccattcc aaaagcgaac
    14821 ggctatcagt ctttgcacac ctcgatgatc ggcccgcacg gtgtgccggt tgaggtccag
    14881 atccgtaccg aagatatgga tcagatggcg gagatgggtg ttgccgcgca ctgggcttat
    14941 aaagagcacg gcgaaaccag caccaccgcg caaatccgcg cccagcgctg gatgcaaagc
    15001 ctgctggagc tgcaacagag cgccggtagt tcgtttgaat ttatcgagag cgttaaatcc
    15061 gatctcttcc cggatgagat ttacgttttc acaccggaag ggcgaattgt cgagctgcct
    15121 gccggtgcaa cgcccgtcga cttcgcttat gcagtgcata ccgatatcgg tcatgcctgc
    15181 gtgggcgcac gcgttgaccg ccagccttac ccgctgtcgc agccgcttac cagcggtcaa
    15241 accgttgaaa tcattaccgc accgggcgct cgcccgaatg ccgcgtggct gaactttgtc
    15301 gttagctcaa aagcgcgcgc caaaattcgt cagttgctga aaaacctcaa gcgtgatgat
    15361 tctgtaagcc tgggccgtcg tctgctcaac catgctttgg gtggtagccg taagctgaat
    15421 gaaatcccgc aggaaaatat tcagcgcgag ctggatcgca tgaagctggc aacgcttgac
    15481 gatctgctgg cagaaatcgg acttggtaac gcaatgagcg tggtggtcgc gaaaaatctg
    15541 caacatgggg acgcctccat tccaccggca acccaaagcc acggacatct gcccattaaa
    15601 ggtgccgatg gcgtgctgat cacctttgcg aaatgctgcc gccctattcc tggcgacccg
    15661 attatcgccc acgtcagccc cggtaaaggt ctggtgatcc accatgaatc ctgccgtaat
    15721 atccgtggct accagaaaga gccagagaag tttatggctg tggaatggga taaagagacg
    15781 gcgcaggagt tcatcaccga aatcaaggtg gagatgttca atcatcaggg cgcgctggca
    15841 aacctgacgg cggcaattaa caccacgact tcgaatattc aaagtttgaa tacggaagag
    15901 aaagatggtc gcgtctacag cgcctttatt cgtctgaccg ctcgtgaccg tgtgcatctg
    15961 gcgaatatca tgcgcaaaat ccgcgtgatg ccagacgtga ttaaagtcac ccgaaaccga
    16021 aattaatgtt ttatgaaccc aacacgttat gcacgcatct gcgaaatgct cgccaggcgg
    16081 cagcctgatc tgaccgtctg catggagcag gtccacaaac ctcataacgt ttctgcgatt
    16141 attcgtaccg cagatgccgt tggcgtacat gaagttcacg ccgtctggcc tggtagccgc
    16201 atgcgcacca tggcttcggc agcggcgggt agtaacagct gggtacaggt gaaaacacac
    16261 cgcaccattg gcgatgccgt cgctcatctc aaaggccagg gcatgcagat tctggcaacc
    16321 catctttctg ataatgctgt cgatttccgc gaaattgatt acactcgccc gacctgcatt
    16381 ttgatgggac aggagaaaac gggcatcacg caggaagcat tggccctggc ggatcaggac
    16441 atcatcattc cgatgatcgg catggtgcag tcgctgaatg tttccgttgc ctcagccctc
    16501 attctttacg aagcccagcg tcagcggcaa aatgcaggca tgtacctgcg tgaaaacagc
    16561 atgttgccgg aagcagagca acaacgcctg ttgtttgaag gcggctatcc ggtgctggcg
    16621 aaagtcgcaa aacgcaaagg cctgccttat ccccacgtca atcagcaagg cgagatcgaa
    16681 gctgatgccg actggtgggc tactatgcag gctgcagggt aagtgccatg aaaggtcgcc
    16741 tgttagatgc tgtcccactc agttccctaa cgggcgttgg cgcagcactt agtaacaaac
    16801 tggcgaaaat caacctgcat accgtgcagg atctactctt acaccttccc ctgcgctacg
    16861 aagatcgcac ccatctctac cccatcggag aactactgcc gggcgtttat gccacggtgg
    16921 aaggcgaagt gctgaactgc aatatctcct tcggcggtcg gcggatgatg acctgccaga
    16981 tcagcgacgg ttccggcatc ctcaccatgc gctttttcaa tttcagcgcg gcaatgaaaa
    17041 atagcctggc gacgggccgc cgtgtactgg cttatggcga agcaaagcgc ggtaaatatg
    17101 gtgcggagat gatccacccg gaataccgcg tgcagggcga tctcagcacg ccagaattac
    17161 aggaaacgct cacgccggtt tatccaacaa cggaaggcgt aaagcaggcc acgctgcgta
    17221 aattaaccga ccaggcgctg gatctgctcg acacctgcgc cattgaagaa ctcctgccgc
    17281 cggaactgtc acaaggaatg atgacgctac cggaagcgtt gcgcactttg caccgcccgc
    17341 caccgacgct acagcttagc gatctggaaa ccgggcagca tccggcgcaa cgtcgtctga
    17401 ttctggaaga actgctggcg cacaacctca gcatgttagc cttacgtgcc ggagcacagc
    17461 gttttcatgc ccagccgctg agcgccaatg acacgctgaa aaataaactc ctcgccgcct
    17521 taccgttcaa gccaacgggc gcacaggcac gcgtagtggc ggagatcgag catgatatgg
    17581 cgctggatgt gccgatgatg cgcctggtac agggcgatgt aggttccggt aaaacgctgg
    17641 tcgccgccct cgccgcgctg cgtgcgattg cccacggcaa acaggtggca ctgatggcac
    17701 caaccgaatt actcgccgag cagcacgcca ataacttccg caactggttt gcaccgctcg
    17761 gtatcgaagt gggctggctc gccggtaagc agaaaggcaa agcgcgactg tctcagcagg
    17821 aagccatcgc cagcggtcag gtgcagatga ttgtcggtac acacgccatc ttccaggaac
    17881 aggtgcagtt taacggcctg gcgctggtga ttatcgacga acagcatcgt tttggcgtgc
    17941 atcagcgtct ggcattgtgg gagaaaggcc agcagcaggg cttccatccg catcagttga
    18001 tcatgaccgc cacgccgatc ccccgcacgc tggcaatgac ggcgtatgcc gatctcgata
    18061 cctcggtgat agatgagctg ccgccaggcc gcacgccagt gactacggtc gctattcctg
    18121 atacccgccg taccgacatc attgaccgcg tgcgccacgc ctgcataact gaaggtcgtc
    18181 aggcgtactg ggtttgtacg ttgattgaag agtcagagtt actggaagcg caggcggcgg
    18241 aagctacctg ggaagagttg aaactggcgc taccagaatt gaatgttggc ctggtacacg
    18301 ggcggatgaa accagccgag aaacaggcgg tgatggcgtc gtttaaacaa ggtgagctac
    18361 atctgctggt tgccactacc gttattgaag tcggtgttga tgtgcctaac gccagcctga
    18421 tgattatcga aaaccctgag cgtctgggtc tggcgcagtt acaccagctg cgtgggcgcg
    18481 taggtcgtgg cgcggtggct tctcactgcg tgctgctcta caaaacgccg ctttctaaaa
    18541 cggcgcaaat tcgcctgcaa gtgctgcgcg acagtaacga cggttttgtg attgcgcaaa
    18601 aagatctgga gattcgcggc cctggcgaat tgttaggcac gcgtcagacg ggtaatgctg
    18661 aatttaaagt ggcggattta ctgcgcgatc aggcgatgat cccggaagtt cagcgcctgg
    18721 cgcgacatat tcacgaacgt tacccacaac aggcaaaagc cttgatagaa cgctggatgc
    18781 cggagacaga acgttactcg aatgcgtaaa aggcggcggt gagaagaccg ccgcttcatc
    18841 ggttaaccgg caaaaatcgg caacattaaa tacagcttaa tcaccagcgc attgacgata
    18901 tcgataaaga acgcaccgac catcggcacc accaaaaacg ccatgtgcga cgggccaaag
    18961 cgttcagtga tcgcctgcat gttggcgatt gccgttggcg ttgcaccgag gccaaaacca
    19021 cagtgacccg cagccagcac tgccgcatcg tagtttttgc ccatcatgcg ccaggtaacg
    19081 aagatggcat acaacgccat gaagatggtc tgtaccacca gaatcgccag catcggcagt
    19141 gccagcgaag ccagctccca cagtttcagc cccatcaacg ccatcgccag gaacaacgac
    19201 aagcttacgt tacccagcac ggataccgca cgctcaaaga cgcggtaaaa gcccattatt
    19261 gacagaccgt tgctcagaat cacgccaaca aacagtacac agacgaaggt cggcagttca
    19321 aaagcagtgc cagccaaaag ttgcgcaaca attttcccca ccgtcaggca gatagcaatc
    19381 agcgcgatag tttcaatcag caccaacgag gtgatcatgc gtcccacatc cggcttttca
    19441 aacgccgtcg ggacttcctg gtcatccgga ataccgttcg gcgtggtgga gtgtttcacc
    19501 agatagcgcg ccaccggacc gccaatcaag ccgcccagca ccagaccgaa cgttgcacag
    19561 gccatagcca cttccgtcgc attggtgaag ccataacgtt caatgaacaa tttactccac
    19621 gcagcgcccg taccgtgacc gccggaaagc gtaatagaac cggccaacag ccccatcagc
    19681 ggatcaagcc ctaacaggct agccatacca atgccaatgg cattttgcat caccaacaga
    19741 ccaacaacca caatcaagaa gatgccaacc acacgcccac cggcacgcaa actggcaatg
    19801 ttggcgttca ggccaatggt ggcgaagaaa gccagcatta acggatcgcg cagggacata
    19861 tcaaagttga cttcccagcc catgcttttt ttcagtacta gtagcgccag cgccaccaac
    19921 aaaccacccg caacaggttc cggtatggtg tatttcttca aaaaggagac ggaatggacc
    19981 aacttacgcc cgagcagcaa cgtcagcgtt gcggcaacaa gcgttgctaa agtatcgaga
    20041 tgaaacatag ttactccttt gtatccgcat cactctttca tactcgtcat acttcatcgc
    20101 gcagtggcaa cttcatgaac tatgacgggt atcgagttat gctttcctgg gtcaaattct
    20161 cagctgttaa tgaacaaatg gcatggattt taagcagaaa aataccaaaa gttatataaa
    20221 aaagtgcatt tatctcattt aaatatttag atgaggatgt ggcaaacgtt tgcttttctc
    20281 tttcggtcag ctaaaatgcc cgctttgcta ccacgggatt gttttcaatg tctgtttcca
    20341 ccctcgagtc agaaaatgcg caaccggttg cgcagactca aaacagcgaa ctgatttacc
    20401 gtcttgaaga tcgtccgccg cttcctcaaa ccctgtttgc cgcctgtcag catctgctgg
    20461 cgatgttcgt tgcggtgatc acgccagcgc tattaatctg ccaggcgctg ggtttaccgg
    20521 cacaagacac gcaacacatt attagtatgt cgctgtttgc ctccggtgtg gcatcgatta
    20581 ttcaaattaa ggcctggggt ccggttggct ccgggctgtt gtctattcag ggcaccagct
    20641 tcaactttgt tgccccgctg attatgggcg gtaccgcgct gaaaaccggt ggtgctgatg
    20701 ttcctaccat gatggcggct ttgttcggca cgttgatgct ggcaagttgc accgagatgg
    20761 tgatctcccg cgttctgcat ctggcgcgcc gcattattac gccgctggtt tctggcgttg
    20821 tggtgatgat tatcggcctg tcgctaattc aggtggggct gacctccatt ggcggcggtt
    20881 acgcagccat gagcgataac accttcggcg caccgaaaaa tctgctgctg gcaggcgtgg
    20941 ttttagcctt aattatcctg cttaaccgtc aacgtaaccc ttacttacgc gtggcctcac
    21001 tggtaattgc gatggcggcc ggatatgcgc tggcgtggtt tatgggcatg ttgccagaaa
    21061 gcaacgaacc gatgacgcaa gaactgatta tggtgccaac gccgctctat tacggtcttg
    21121 gcattgaatg gagtctgctg ctgccgctga tgctggtctt tatgatcact tcgctggaaa
    21181 ccattggcga tatcacggcg acctctgacg tttccgaaca gccagtgtcc ggtccgctgt
    21241 acatgaaacg cctgaaaggc ggcgtgctgg caaacggcct gaactcgttt gtttcggcgg
    21301 tgtttaacac cttcccgaac tcctgcttcg ggcaaaacaa cggagtgatc cagttgactg
    21361 gtgttgccag ccgctatgtc ggttttgtcg tcgcgctgat gttgatcgtg ctgggtctgt
    21421 tcccggcagt gagcgatttt gtacaacaca ttccagaacc ggttctgggc ggcgcaacgc
    21481 ttgtaatgtt tggcaccatc gccgcctccg gtgtgcgtat cgtttctcgt gagccgctga
    21541 accgtcgggc gattctgatt atcgcgttgt cgctggcggt tggtctgggc gtgtctcagc
    21601 agccgctgat tttgcagttt gcccctgaat ggctgaaaaa cctgctctcc tccgggatcg
    21661 ccgcgggcgg tattactgcc atcgtgctga atctgatttt cccaccagaa aaacagtaat
    21721 tcactggcgg cggtaagata attaccgccg cagtctcacc tctttcacca ttcccgcctt
    21781 gaggattgcg cgtaaatcgt gcataactcc cttatgtgca tttcacggga tggaagacca
    21841 tgaaatttat tgggaagctg cttctctaca ttctcatcgc tctgttagtg gtgatcgctg
    21901 gcctctattt tcttctgcaa acccgctggg gagcagaaca tatcagcgca tgggtttccg
    21961 agaatagcga ctatcatctg gccttcgggg cgatggatca ccgtttttcc gcgccatctc
    22021 atatcgtgct ggagaatgtc acgtttggtc gtgatggtca gcccgcgccc ctggtggcaa
    22081 aaagtgtcga cattgcgcta agcagtcgcc aactgaccga accacgccat gtcgatacca
    22141 tcctgctgga aaacgggacg ctgaatctca ccgaccagac cgcgccgcta ccgttcaaag
    22201 ccgatcgtct gcaactgcgt gatatggcgt ttaatagccc gaatagcgaa tggaaactga
    22261 gcgcgcagcg ggtaaatggc ggcgtggttc cgtggtcacc agaagccggt aaagtgctgg
    22321 gtacgaaggc gcagattcag tttagtgccg gatcgctttc gctcaatgat gttcctgcca
    22381 ccaatgtact gattgaaggc agtattgata acgatcgcgt tacgctgact aacctgggtg
    22441 ccgacatcgc ccgcgggaca ttaaccggaa acgcgcagcg taacgccgac ggcagctggc
    22501 aagtggaaaa tctgcgcatg gcggatatcc gtctacaaag cgaaaaatcg ctaaccgact
    22561 tctttgcgcc attacgctct gtcccgtcgt tgcagattgg tcgcttggaa gtcatcgatg
    22621 ctcgtctgca aggtccggac tgggcggtga ccgacctcga tctcagcttg cgcaacatga
    22681 ccttcagtaa agatgactgg cagacacagg aaggcaaact gtcgatgaac gccagcgagt
    22741 tcatttatgg ttcgctgcat ttatttgacc cgattataaa cgcggaattt tccccgcagg
    22801 gcgtagcgct gcgccagttc accagccgct gggaaggggg tatggtcaga acgtcaggga
    22861 actggctgcg tgacggtaaa acgttgatcc ttgatgatgc ggcaattgcc gggctggaat
    22921 ataccttgcc gaaaaactgg caacagttgt ggatggaaac gacacccggt tggttaaaca
    22981 gcctgcaact gaagagattt agcgccagcc gcaatctgat cattgatatc gaccctgact
    23041 tcccgtggca gctcaccgcg ctcgatggtt acggtgccaa cctgacgctg gttaccgatc
    23101 ataaatgggg cgtctggagt ggctcggcga atctgaatgc cgccgccgcg acattcaatc
    23161 gtgttgatgt tcgtcgcccg tcgctggcgc tgaccgccaa cagcagcacg gtgaatatca
    23221 gcgaactgag tgcatttact gaaaaaggca ttctggaagc caccgcaagt gtttcacaaa
    23281 cgccacaacg tcagacccat atcagcctga atggacgcgg tgtgccggtg aatattttgc
    23341 aacaatgggg atggcctgaa ttacagttga ctggcgacgg caatattcag cttaccgcca
    23401 gtggagatat tcaggccaat gccccgctga aacctacggt tagcgggcaa ttgcatgccg
    23461 tgaacgccgc aaagcagcaa gtgactcaaa ccatgaatgc gggcgtcgtt tccagcggtg
    23521 aagttacatc gacggagccg gtgcggtaaa catcaatctc gccgggtacg actcagacgt
    23581 acccggcatt ccgtcaatag attacaacgt aattgtcagc gcattccctt gaggcgtcac
    23641 caccagcccc tgctcacttt cagcctgcga accgccttgc agaccattta ctttcacaac
    23701 attgcgcaag cacagcgtcc agttcttcgc ctcgcccgca ccagtcacag taatcgtgtt
    23761 gccagtacgt gctgctttta aagtaaagat caccgatccg tcagcagcag gcacttcaca
    23821 gacggcttca tgcccgtctt gcagattaaa gaggtggaat gccgtgcctt cgtgccacac
    23881 gtaatcggga cgttgatcgt tgttgcccag cgccagtaga gtgttatcac gcacataaac
    23941 gggcagactc aggaagccgt gctgctgttt atgccagcga ctaccgtcga gttcatcgtt
    24001 gtgccacagg tgtgtccagc gaccttccgg caggtagaac tgcacatcgc ccgcttcagt
    24061 gaacaccggc gcaaccatca cgttgtcgcc taacatgtat tgacggtcaa ggtaatcaca
    24121 agccggatcg tccgggaact ccatcatcat ggcccgcatc atcggcgtac cccgcgcgtt
    24181 cgcacgcgca gcttcacgat acagatacgg catcatgcgg catttcagtt gcgtgaagaa
    24241 gcgcaccaca tcacaggact catcatcgta ggcccacggc acacgataag atttgctacc
    24301 gtgtaaacgg ctatggctgg agagcaaacc aaacgcgcac cagcgtttgt aaacgtgcgc
    24361 cggagcggta ttttcaaagc cgccgatatc gtggctccag aagccaaaac ctgaaaggcc
    24421 aatagacaaa ccaccgcgca ggctttccgc cattgattcg tagttagcgt aacaatcgcc
    24481 accccagtgt accgggaatt tctgcgcacc gacggaggcc gagcgggcaa acaagacagc
    24541 ttcttcctca ccaacggtgt ccttgagcac gttccacacc agttcgttgt agatgtacgc
    24601 ataatggtta tgcattttct gcggatcgga accgtcaaac cactgaacat cagttgggat
    24661 acgttcgcca aagtcggtct taaagcaatc aacgcccatc gcgaccagac ctttcagttt
    24721 gtcggcgtac catttgcagg catccggatt ggtaaagtca taaatcgcca gacctggctg
    24781 ccatttatcc cactgccata gcgaaccgtc cgggcgtttg agtaaatagc ctttctcttg
    24841 taactcttta aagacggggg atttttgacc gatatagggg ttaatccaga cgcagatttt
    24901 cagccctttc gctttcaggc ggcggatcat cccttccggg tcagggaaag tcagcgggtc
    24961 ccactcaaaa tcgcaccact ggaaggcttt catccagaaa cagtcaaagt ggaaaacatg
    25021 cagcggcaga ttgcgttccg ccataccatc gataaagctg tttaccgtcg cttcgtcgta
    25081 gttggtggta aatgaagtgg ttagccacag gccgaaggac cacgggggcg gcagcgccgg
    25141 acgaccagta aagcgggtat aacgatcaag taccgctttc ggcgtcgggc cgtcgataac
    25201 aaagtattcg agatattcac tctcaacgct gaactgcact ttggagactt tctccgatcc
    25261 cacttcaaaa gagacacact ggggatgatt gaccagtacc ccataaccac ggttagtcat
    25321 gtagaacggg atatttttat acgcctgttc agtacttgtg ccgccgtccc ggttccaggt
    25381 ctctaccgtc tggccattgc gcaccagggc agtaaagcgc tctcccagac cgtaaactgt
    25441 ttcgccaacg ccaagatcaa gccgctcaaa catataattg cgttgattat tcgtgtcctg
    25501 cacgtagcca ttatttttca cctgactacc ggtaatacgt tcgccgttgc gcagaaaatc
    25561 cagtgaccag aactcacctt tgctgacacg cgcgcttaag ttgccacttt taaactcagc
    25621 ataacgttct gtgttttcga ttgtgacctt cacgtcctgc aaaatattga gcggataatg
    25681 aggaccgtta ttcagcgccc cctgaaaata ctcaatccgc acaccgacaa taccttcctg
    25741 tggggagaaa aagcgcagcg taaataaagg cgtatcaagc tgccaggtac gttcacgcac
    25801 atcacggggg gcagcataga ccaccatttc attatcctgc tgttcaacct cgaatacctg
    25861 aagcgggtga atcaaattga gcccaggttg aatcaaccag tttccatcgc taattttcat
    25921 tgccgttcct tagttctgca cttcttgaga ggtgaattgt tgctggcaac ggcgtttacc
    25981 ctgagccagc tgttccataa cggttttcag attgtgcgtc gtgagtgagt agtagcgttt
    26041 agcgataatc gcgctcagca aataacagat cgccggaaca atcgtgaata gcgcaataat
    26101 gatgctaatc gtggcgctgt tctgcgcttt ttctgccgca tcatatccgc cataagccag
    26161 catccagccg ataagagcgc cgccgaaggc caaccccagt ttgagcacaa acagcgtgcc
    26221 agcaaaactg atcccggtca ggcgtttacc attgcaccac tcgccgtagt cgacggtatc
    26281 gaacatcatt acccactgga taggtgtcac cagttgatgc aacacaccaa tcacgaagat
    26341 gaagacaaac atagtgatgc tggcctgcat gggaacaaag aacatcgcga ggctaatcac
    26401 tgccagcagg gcgttcgtcc accagaagat agtgacttta catttccagt cggtcagagg
    26461 ttttgccagt gcggaaccaa tcaggttacc cacgcaataa gtggtgagaa aagcgacaaa
    26521 cacttccggc gtgcccaaaa tccatgtgac gtaatacatc atcgccccac cgcgtacgca
    26581 caccgccagg atattgaaaa tggttagtaa accgacaatc cgccactggt cgttttgcca
    26641 gatatcacgt aaatcttccc gcatagacgt tgttgtaggt ggtgcttcaa cgcgttcttt
    26701 agtggtgaag aaacaaaatg ccagcatcat taatgccacc acggaaagga ccacgatacc
    26761 gccctggaaa ccgagtggtt tattatcacc gccaattaaa ttaaccagtg gcatcatcag
    26821 aacagtagaa agcatgcctc ccgcggtcgc cagcacaaaa cgccaggatt gcagcgagat
    26881 acgctgagtc gggtcattgg tgattacacc acccaatgcg cagtaaggga tattgacgac
    26941 ggtataaagt aaggtaagtt agggtgtaag taattgctgc atagatcatt ttgccgttca
    27001 tactgagatc tggcgtgcta taggccagta cacagacgat cccgaatggc agtgcgccaa
    27061 acagtaccca cggacgaaat ttaccccagc gagagcgcgt tcgatcggcc aacaacccca
    27121 tgcaaggatc ggaaatcgca tccagtgcac gagcgaccaa aaacatggtt ccgacaaatc
    27181 cggcaggaat gccaaaaata tcggtataaa agaacatcat atataacatt acgttatcga
    27241 aaataatgtg gctggcggcg tctcccatgc cataaccaat tttctcttta acggacaaca
    27301 cttcactctt catctgcttt tccttgatgc tataggggtg ctgagaccga taacgcattt
    27361 tgtaaaccat ccctgtgaga gcgaatattc cgatatctgg ttattaattt atgattcttg
    27421 ttttatgtga tcgtggtagc gttaattccg ctcatatatc attgtaaaat atgggtttta
    27481 tataaattat aatgctttcg tgataatacg ctgcgtttat taggcggaaa aaactgatct
    27541 gggggatgta gaaactcaag gaagtagcta taatgcgccc cgcctccatg tagcaatcga
    27601 ggcgcggaag atcgtcgtct ccggtgaggc ggctggactt caaatccagt tggggccgcc
    27661 agcggtcccg ggcaggttcg actcctgtga tcttccgcca gtttacctct tctgaactct
    27721 cccaatgtca ataaatctct tctaatccct tgtatatcag tatttatttg tatcctgagg
    27781 tcaacctact tcaccccgta tcaagtttct tttgggggta ctttaggggg tgttatttcc
    27841 tattgaaata caagcacccc cataatagga aaaatgtgat acataacaac ggcttttaac
    27901 aatatctttg ttatacaagg ggtgtattgg gggtatcagg ttagttactg gcagaaatcc
    27961 ccctcaggaa catgaattca tcaaaagccg gaggatgtca tggcattaac cgatatcaaa
    28021 gtgaaaaccg caaaacccaa agacaaaccc tataagcttg ctgatggcgg cggtatgtat
    28081 ctactgatta acactaatgg ttctaaatac tggcgcatga agtaccgctt tgctggtaaa
    28141 gaaaagatgc tttctattgg cgtatacccc gatgtaacgc tagccgatgc tcgcgagaaa
    28201 cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc caggggaggc aaaaaaggaa
    28261 gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg aggctattgc tcgcgaatgg
    28321 caccagttaa aagctgatcg gtggtcatta cgctatcgtg atgaaatcat tgataccttt
    28381 gaaaaagaca tttttcccta cattggcaag cgccctattg ccgaaatcaa accaatggag
    28441 ttactggaag cactgcgtaa aatggaaaaa cgtggagctc tggagaaaat gcgcaaggtc
    28501 cgccagcgct gtggtgaggt gttccgctat gcaattgtta ctggccgggc agactataac
    28561 ccagcccccg atcttgccag tgctttagct acaccgaaaa aagtacattt tccctttctt
    28621 actgctaatg aacttcccca cttcctcaac gatctggcgg gttataccgg aagtataatc
    28681 actaagacag ctactcagat cattatgtta accggcgtac gaacacaaga actgcgtttt
    28741 gcacgctggg aagatatcga ttttgagaca aaattgtggg agatcccggc agaagtaatg
    28801 aagatgaagc gacctcatat tgtgccgctc tctgagcagg tcattatgct atttaagcag
    28861 cttgaaccaa tctcaaaaca tcatccccta gtctttatcg gtagaaatga tccccgcaaa
    28921 ccaattagta aggaaagcat taaccaagta attgaattac ttggatataa gggaagactc
    28981 acagggcacg gtttcagaca caccatgagt acaattctgc atgaacaagg ctttaattcc
    29041 gccttgattg aaatgcagtt agctcatgtg gataaaaatt ccatcagggg tacctataat
    29101 catgccctgt atctcgatgg ccgccgtgaa atgatgcaat ggtacgctga ttacattgat
    29161 tcactctcca gccgggaaag ttaagtcaga aatacttttc cctgatgttt tcctcaatta
    29221 agtattttat cctctggcct acaaggctgg aggatttttg ttattatatt atttttttgc
    29281 gtcttcaaac ttattacaaa aacataatta aaaacaaatt gacattttta aattggcaat
    29341 tacaaaatac agccttgcag ttatttcgat acctttagca aaggaaaagt tgattcagat
    29401 ataatttagc atttcaatca tctgacatca ttataaaaaa tgagattcac ataaagataa
    29461 aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag caatattcat tcggtgccaa
    29521 gacaggactt gatacacaga atctcaactt ttatgctctc agactatatc accacatgtt
    29581 ttacaggctc agaaaatgaa cgatagatta tgctttgaag tgcacgataa caagggttat
    29641 ttcgtatttc cggatacctg gtttggccca ttgctggggg agtttgaaga agtgcttgat
    29701 gcatacgacg ctgatgaaat cagcgagacg agttacatca acaaactccg acgccttgct
    29761 caacgggagc cggactttat tgatatacac gctcatctgg catatgcatt tctggaacag
    29821 aatgccccgc gtaaggcact gaacgccgct cttaaaggac tggcggcagg taatcgtatc
    29881 atcccggaaa gtttctgtgg tgaaataatc tggatgcatc ccgaaaaccg gccttatctg
    29941 cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac agcgccatca ggatgcggtt
    30001 atgctcactg ataaaatact ggcatacaat cctgaagata atcagggggc tcgctggttg
    30061 ttgggttcag agctgttacg caccggcgat catgagcgag ccttcagtgt actgaaagag
    30121 catgctgatg aattttcacc ttactggtat gaactcggtc tcctgcattt tctgaacggg
    30181 gaacatgtta aggccgccac agcttttcgc catggctttg ccacaaacac ttacatcgca
    30241 gaaatgctgt gcggaaatct gcatccattt ccacttgctg tgtggcatga cttctccggc
    30301 tctctggata cggcagaaga ttactatgca acatactcac cattatgggg acaatatccg
    30361 gaagcattgc tgtttgtaaa ctggctctat aaccattcct ccgtgctgca tgaacgatca
    30421 gaaatcatta aatgcgccga aatgctgatt caggaagatg atttcgaaat atgcgaaagt
    30481 attttaagac agcaggagca cctgtggaaa agaattgaca agacactttc tgaggaaatt
    30541 gtacagaaat gcagaaatat gaacggtgaa tatatctggc cctggatatt gcccttttcc
    30601 gcagcaggga tgaaacattc aagaatccag tatcagtaga tattgcatta gtgtaacttt
    30661 aaaacaagta ataatttatt atccaataat aacactgtgt tccttcccca ggtgattacc
    30721 ctggggttat tttctgaaag tttcctttgc aaaaggatag cgttgtaaat tactttaacg
    30781 aattactcca tgaactcatt aaagttcttt cgtaatactt tatcgtcaaa cacgttatag
    30841 ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa tcccctcctc tggcgttttc
    30901 ggttgaactg gtatccccgt ccaacacagg cagccattat aaacagtaaa aaattgtatt
    30961 cgctgttatg aaatcaatag tcctgacgta ctgttgaatg tgcttgtgtt ctgaacctcg
    31021 tacaaccagt acatttgata tcgaattata aacaaaacat tgcatccaac ttttttaata
    31081 tggacaatag ttgtccatac ggtggtttta tattcgataa atcaatggat ttcattgagt
    31141 cacaggactg gtaatggatg aaaatgcttt aaggtttgcc tcatactggc gcaactcgct
    31201 tgcagatgct gagtcaggaa agggctgttt tgaacggaaa gacgcccaaa atttcactca
    31261 ctggcatggg atagcggcgg gacgtcttga cgaagcgatt gtcaataaat tttttaaggg
    31321 agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc ccaaaagttt atttccggtt
    31381 actgcagcat ggtaaggacc gttccgcagg cacgcctgat attgttacac cgatagtgac
    31441 gccagccttg ctaagccgtg agggtttttt atatccgacg ccagcgacct ccattcccag
    31501 agacctgctt gaacctttgc caaaaggagc attttcgatt ggtgagattg ggcagtatga
    31561 caaatacaag acgacccata ctgatcctac ccacgtaatg tggacacggc cctaagcgag
    31621 attatggttt tcaaattgtt ccgggctgag accgccacag gcactgtgac gacgccagcg
    31681 attgtaatca cactcgatat aattaaacac cgttgcccgc attatttccc ggctgctaaa
    31741 gcgttccccg tggatacatt ccaccttcag cgaatgaaag aagctttcca cacaggcatt
    31801 gtcataacag tagcctttcg cactcatact gccacgcagg ttgtgtcgct tcagcattgc
    31861 ctgataatcc cctgaacagt attgaccacc acggtccgta tgaacaatga cgctttccgg
    31921 gcgtcttctc cgccacaacg ccatttgcag tgcatcacag gccagttgtg ctgtcattcg
    31981 cggtgacatc gaccagccaa tgacggcgcg tgaccacagg tcgatgacta ctgcgagata
    32041 caaccagccc tcatcggtac gcaagtacgt gatgtcaccc gcccacttct ggtttcgggc
    32101 cactggcgct gaagtcctgc tccagcagat tctccaatac gggcaggcca tgtgcacggt
    32161 agctgaccgg gctgaacttc cggctggctt tcgcccgcag cccctgacga cgcaggctgg
    32221 cggcaatggt tttaatattg aactccggca gttcgtcagc aaggcgggga gcaccgtatc
    32281 gctgctttgc ctcaaagaat gccttatgga cagcggcatc gcaggtgagc cgaaactgtt
    32341 ggcgcaggct catctggtga cgacgcctga gccagacata ccagccgctg cgggcaaccc
    32401 gaagtacacg acacatcgct ttgatgctga actctgcccg atgattttcg ataaagacat
    32461 acttcatttc aggcgcttcg cgaagtatgt cgcggccttt tggaggatgg acagttcctc
    32521 agcctgctcc gccagttgtc gtttaaggcg gacattttca gcggccagtt cgctttcgcg
    32581 ctctgacgaa ctcatttgtt gctgctgttt actgcgccag gcataaagct gagattcata
    32641 caggctgagt tcacgggctg cggcggccac accgatgcgt tcagcgagtt tcagggcttc
    32701 gttacgaaat tcaggcgtat gttgtttacg gggcttcttg ctgattgata ctggttttgt
    32761 catgagtcac ctctggttga gagtttactc acttagtcct gtgtccacta ttggtgggta
    32821 agatcaccct ccattaaaac taaaacgggc gctttctgat atatcatctg taagcgcagt
    32881 aataccatta gtagcacctc tcggcggcgc attgattatt gaatattata actggaatgt
    32941 tatatttctt gttatgatgc tgttcagtat tataataatg ttattatcac caaagataac
    33001 tcatgcaaat gagaaagtaa tatgtcaaaa cagtgcatcc tttgtgacca aagagtttat
    33061 ctgcggcact atacttgtct cattaatact cagttctcta ttttgctaca tat
//
LOCUS       DAJVQH010000031        32902 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJVQH010000031 DAJVQH010000000
VERSION     DAJVQH010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.031"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            16..4548
                     /locus_tag="OQM87_001566"
     CDS             16..4548
                     /locus_tag="OQM87_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCS1507740.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDVWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSS
                     SDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTP
                     DDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIA
                     DALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALLWDKTANGGAGAYNAS
                     HDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEEN
                     GAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALG
                     SSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEA
                     HDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIG
                     LNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEF
                     SVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVT
                     NIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAE
                     EENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNIT
                     LGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQRMVEMDNKLSKTESKL
                     SGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGST
                     NSQGEYSAALGAGIQW"
     gene            4916..6571
                     /gene="lldP"
                     /locus_tag="OQM87_001567"
     CDS             4916..6571
                     /gene="lldP"
                     /locus_tag="OQM87_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCS1507741.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6571..7347
                     /gene="lldR"
                     /locus_tag="OQM87_001568"
     CDS             6571..7347
                     /gene="lldR"
                     /locus_tag="OQM87_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCS1507742.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7344..8534
                     /gene="lldD"
                     /locus_tag="OQM87_001569"
     CDS             7344..8534
                     /gene="lldD"
                     /locus_tag="OQM87_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCS1507743.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8730..9203
                     /gene="trmL"
                     /locus_tag="OQM87_001570"
     CDS             8730..9203
                     /gene="trmL"
                     /locus_tag="OQM87_001570"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCS1507744.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9256..10077)
                     /gene="cysE"
                     /locus_tag="OQM87_001571"
     CDS             complement(9256..10077)
                     /gene="cysE"
                     /locus_tag="OQM87_001571"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCS1507745.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10157..11176)
                     /gene="gpsA"
                     /locus_tag="OQM87_001572"
     CDS             complement(10157..11176)
                     /gene="gpsA"
                     /locus_tag="OQM87_001572"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCS1507746.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11176..11643)
                     /gene="secB"
                     /locus_tag="OQM87_001573"
     CDS             complement(11176..11643)
                     /gene="secB"
                     /locus_tag="OQM87_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCS1507747.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11706..11957)
                     /gene="grxC"
                     /locus_tag="OQM87_001574"
     CDS             complement(11706..11957)
                     /gene="grxC"
                     /locus_tag="OQM87_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCS1507748.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12099..12530)
                     /locus_tag="OQM87_001575"
     CDS             complement(12099..12530)
                     /locus_tag="OQM87_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCS1507749.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12775..14319
                     /gene="gpmM"
                     /locus_tag="OQM87_001576"
                     /gene_synonym="pgmI"
     CDS             12775..14319
                     /gene="gpmM"
                     /locus_tag="OQM87_001576"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCS1507750.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14329..15612
                     /gene="envC"
                     /locus_tag="OQM87_001577"
     CDS             14329..15612
                     /gene="envC"
                     /locus_tag="OQM87_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCS1507751.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15616..16575
                     /locus_tag="OQM87_001578"
     CDS             15616..16575
                     /locus_tag="OQM87_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCS1507752.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16562..17596)
                     /gene="waaH"
                     /locus_tag="OQM87_001579"
     CDS             complement(16562..17596)
                     /gene="waaH"
                     /locus_tag="OQM87_001579"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCS1507753.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17835..18860)
                     /gene="tdh"
                     /locus_tag="OQM87_001580"
     CDS             complement(17835..18860)
                     /gene="tdh"
                     /locus_tag="OQM87_001580"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCS1507754.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18870..20066)
                     /gene="kbl"
                     /locus_tag="OQM87_001581"
     CDS             complement(18870..20066)
                     /gene="kbl"
                     /locus_tag="OQM87_001581"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCS1507755.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20341..21198)
                     /gene="yibB"
                     /locus_tag="OQM87_001582"
     CDS             complement(20341..21198)
                     /gene="yibB"
                     /locus_tag="OQM87_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCS1507756.1"
                     /translation="MKSSTTIITAYFDIGRGDWTTNKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKSYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21502..22434
                     /gene="rfaD"
                     /locus_tag="OQM87_001583"
     CDS             21502..22434
                     /gene="rfaD"
                     /locus_tag="OQM87_001583"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCS1507757.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22444..23490
                     /gene="rfaF"
                     /locus_tag="OQM87_001584"
     CDS             22444..23490
                     /gene="rfaF"
                     /locus_tag="OQM87_001584"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCS1507758.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23494..24486
                     /gene="rfaC"
                     /locus_tag="OQM87_001585"
     CDS             23494..24486
                     /gene="rfaC"
                     /locus_tag="OQM87_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCS1507759.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24483..25691
                     /gene="rfaL"
                     /locus_tag="OQM87_001586"
     CDS             24483..25691
                     /gene="rfaL"
                     /locus_tag="OQM87_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCS1507760.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25727..26869)
                     /locus_tag="OQM87_001587"
     CDS             complement(25727..26869)
                     /locus_tag="OQM87_001587"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCS1507761.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26878..27891)
                     /locus_tag="OQM87_001588"
     CDS             complement(26878..27891)
                     /locus_tag="OQM87_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCS1507762.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(27916..28623)
                     /gene="rfaY"
                     /locus_tag="OQM87_001589"
     CDS             complement(27916..28623)
                     /gene="rfaY"
                     /locus_tag="OQM87_001589"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCS1507763.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(28649..29656)
                     /gene="waaO"
                     /locus_tag="OQM87_001590"
                     /gene_synonym="rfaI"
     CDS             complement(28649..29656)
                     /gene="waaO"
                     /locus_tag="OQM87_001590"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCS1507764.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(29699..30496)
                     /gene="rfaP"
                     /locus_tag="OQM87_001591"
     CDS             complement(29699..30496)
                     /gene="rfaP"
                     /locus_tag="OQM87_001591"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCS1507765.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(30489..31613)
                     /locus_tag="OQM87_001592"
     CDS             complement(30489..31613)
                     /locus_tag="OQM87_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCS1507766.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(31610..32668)
                     /gene="rfaQ"
                     /locus_tag="OQM87_001593"
     CDS             complement(31610..32668)
                     /gene="rfaQ"
                     /locus_tag="OQM87_001593"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCS1507767.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         8689 a         8053 c         7770 g         8390 t
ORIGIN      
        1 tagcgagcat cattaatgaa caaaatattt aaagttatct ggaaccctgc gacagggaat
       61 tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga aatctgggcg cagtaagctg
      121 ttaatttctg cgctggttgc gggtggaatg ttgtcgtcgt ttggggcatt ggcgaatgcc
      181 gggaatgaca acggtcaggg tgttgattac ggtagtggat cagctggcga cgtctgggtt
      241 gctataggca aaggggcgaa agcaaatact tttatgaaca ccagtggttc cagtactgct
      301 gtgggttatg acgctatagc tgaaggccaa tatagctctg ccatcgggtc aaaaacccat
      361 gcgattggtg gtgcatcaat ggcctttggg gttagtgcaa tatcagaagg cgatagaagt
      421 atagcactgg gtgcctcttc gtattcattg ggccaatact caatggccct ggggcgtagc
      481 agtgtagcta gcggtactga cagcctcgca tttggcagac aatcacttgc cagcgcagcg
      541 aacgctattg cgataggtgc tgagaccgaa gccgctgaaa atgcaactgc tattggcaat
      601 aatgcgaagg caaaagggac taatagcatg gcaatggggt tcggaagcct tgccgataaa
      661 gtcaatacta tcgcattagg aaatggcagc caggctctgg cagataatgc aatcgccata
      721 ggccagggca acaaagctga tggcgtggat gccatcgctc tgggtaatgg tagccagtcg
      781 agaggcttaa acaccattgc cttaggcaca gccagtaatg caactggtga taagagtctt
      841 gcgcttggta gtaatagcag tgccaacggt attaactctg tcgcgctggg cgcagattcc
      901 attgcggatt tagacaatac cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt
      961 aatgtgaaaa atggcgcgat caagtctgac agttacgatg ccattaatgg ttcacagctt
     1021 tatgccatta gcgactcggt agcaaaaagg cttggaggag gggctgcagt agatgttgat
     1081 gacggtactg ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg
     1141 gctgcgctcg ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac
     1201 agcgctgctc atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc
     1261 acgatttcag cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac
     1321 gatgtcgaag ccaacaccac caatatcacc aacctgacgg attccgttgg tgaccttcag
     1381 gctgatgccc tgctctggaa cgaaactaaa aaggcattca gtgcagctca cggccaggat
     1441 accaccagca aaatcaccaa cgttaaagat gccgacctga cggctgacag cactgatgct
     1501 gttaacggct ctcagctgaa aaccaccaac gatgctgtgg cgacgaatac caccaatatc
     1561 gccaataaca cttccaatat tgccactaac accaccaaca tctctaacct gactgagacg
     1621 gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt attcacggca
     1681 gctcatggca ccgagaccac cagcaaaata accaacgtta aagatggcga cctgacgact
     1741 ggcagcaccg atgccgttaa cggctctcag ctgaaaacca ccaacgatgc cgtggcgacg
     1801 aataccacca atatcgccac taacaccacc aacatctcta atctgactga gacggtgact
     1861 aatcttggtg aggatgcgct gaaatgggat aaggacaatg gtgtcttcac tgcagctcat
     1921 ggcaacaata ccgccagcaa aatcaccaat atcctggacg gcacagtcac tgcaaccagt
     1981 tccgatgcca ttaacggtag ccagctttat gacttaagca gcaatatcgc cacctacttc
     2041 ggcggcaatg cttctgtgaa tactgacggc gtgtttaccg gtccaaccta caaaatcggt
     2101 gaaacaaatt attataacgt cggcgatgct ctgctttggg atgccaccgc aggtaaattc
     2161 agtgccaaac acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa
     2221 atttcagact ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat
     2281 gttgttgatg cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg
     2341 acgtacacca ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat
     2401 accactcctg acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc
     2461 gccgctcacg gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc
     2521 tctgctgcca gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc
     2581 gctgatgcgc tgggtggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact
     2641 tacaccattg cgaacgccga gtacaacaac gtcggtgacg ccctggatgc gcttgatgat
     2701 aacgccctgc tgtgggataa gactgccaat ggcggtgctg gagcctacaa tgccagccat
     2761 gacggtaaag ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc
     2821 gatgcagtga acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa
     2881 attatcaatc agctcgctgg taacaccgac gcaacctata tcgaagaaaa tggcgctggt
     2941 atcaactacg ttcgtactaa cgacaacgat ttagccttta acgatgcaag cgcctctggt
     3001 gtcggcgcta cagctgtagg ttataacgct gtcgcgtctg gtgccagcag cgtagccatt
     3061 ggtcagaaca gcagcagcac cgttgatacc ggtattgcgc tgggtagcag ctccgtttcc
     3121 agccgtgtga ttgccaaagg ttctcgtgac actagcgtaa cggaaaatgg cttggtgatt
     3181 ggttatgaca ccactgacgg cgaactgcta ggtgcattgt caattggtga tgacggtaaa
     3241 taccgccaaa tcatcaacgt agctgatggt tcagaagccc atgacgccgt tacggttcgc
     3301 cagttgcaga acgctattgg agcggtcgcc actacgccaa ccaagtactt tcacgccaac
     3361 tcaacggcag aagactcact ggccgttggt gaagactcgc tggcaatggg tgcgaaaact
     3421 gtcgttaatg gtaatgcagg tattggtatc ggtttgaaca ctctggttct ggctgatgcg
     3481 atcaacggca ttgctatcgg cagtaacgca cgcgcaaacc atgcaaacag tatcgcaatg
     3541 ggtaacggtt ctcagaccac ccgtggtgca cagactggct acaccgccta caacatggac
     3601 gcaccgcaga actctgtagg tgagttttct gtcggtagtg aagacggtca acgtcagatc
     3661 acaaacgtcg cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc
     3721 actgatgagc gcgtagcgca aaatacccag agcattacta acctgaacaa tcaggtcact
     3781 aatctggata ctcgcgttac taatatcgaa aacggtattg gcgacattgt caccaccggt
     3841 agcaccaagt acttcaagac caacaccgat ggcgtagatg ccaacgccca gggtaaagat
     3901 agcgttgcta ttggttctgg ttccattgct gccgctgaca acagcgtcgc actgggtacc
     3961 ggttccgttg cagaggaaga aaatacaatc tctgtaggtt cttctactaa ccaacgccgt
     4021 atcaccaacg tagctgccag tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa
     4081 tcttctgaag caggcggagt gcgctacgac accaaagctg atggttctat cgactatagc
     4141 aatatcaccc tcggtggtgg caacggcagt acgactcgta tcagcaacgt ctccgctggc
     4201 gtcaacaaca acgacgcggt gaactacgcg cagttgaagc aaagcgcgca agaaacgaag
     4261 caatacaccg atcagcggat ggttgagatg gataacaaac tgtctaaaac tgaaagcaag
     4321 ttgagcggtg gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttatacaccg
     4381 ggtgccagca tggcttctat tggtggcggt acttacaacg gtgaatcggc agttgcttta
     4441 ggtgtatcga tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat
     4501 agccagggtg aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaag
     4561 ttagtgtgac taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc aggcaagata
     4621 aggcgctccg ggtcaacaat ctaagggcaa ttctctgatg aggattgccc ttttctttac
     4681 cagacatctc cccccacaag aattggccct accaattctt cgcttatctg acctctggtt
     4741 cacaattccc caattaaaac tcacatcaat attgataata cataacattt agttaaccat
     4801 tcattgtcat tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca
     4861 aggagatgag caacagactc attacacgat gtgcgtggac tctaggagac ctgcaatgaa
     4921 tctctggcaa caaaactacg atcccgccgg gaatatctgg ctttccagtc tgatagcatc
     4981 gcttcccatc ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc
     5041 cgcctcatgg acggtagcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt
     5101 cgctaacgcg ctggcctcgg tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg
     5161 gatcattatt gcagcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat
     5221 cattcgctcg tctattcttt cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg
     5281 tttctgtttc ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac
     5341 cgccgcattg ctggtcggcc tgggttttaa accgctgtac gccgccggac tgtgcctgat
     5401 tgttaacacc gcgccagtgg catttggcgc gatgggcatt ccaatcctgg ttgccggaca
     5461 agtaacaggc atcgacagct ttgagattgg ccagatggtg gggcgtcagc taccgtttat
     5521 gaccattatc gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaaaga
     5581 gacgtggcct gcggtcgtgg ttgcaggcgg ctcgtttgcc atcgctcagt accttagctc
     5641 taacttcatt gggccggagc tgccggacat tatctcttcg ctggtatcac tgctctgcct
     5701 gacgctgttc ctcaaacgct ggcaaccagt tcgcgtattc cgcttcggtg acttgggggc
     5761 gtcacaggtt gatataacgc tggcccacac cggttacacc gcgggtcagg tgttacgtgc
     5821 ctggacaccg ttcctgttcc tgaccgctac cgtaacactg tggagcatcc cgccgtttaa
     5881 agccctgttc gcatcgggtg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta
     5941 cctcaataaa ctggttgccc gtatgccacc agtggtcagc gaggctacag cctatgccgc
     6001 cgtgtttaag tttgactggt tctctgccac cggcaccgcc attctgtttg ctgcactgct
     6061 ctcgattgtc tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct
     6121 gaaagaacta gctctaccca tctactccat cggcatggtg ctggcgttcg cctttatctc
     6181 gaactattcc ggactgtcat caacactggc actggcactg gcgcacaccg gtcatgcatt
     6241 caccttcttc tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc
     6301 atctaacgcc ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga
     6361 tctgttgctg gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca
     6421 atctatcgct atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg
     6481 ctttactgtc aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca
     6541 ggcttatgtc ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac
     6601 gaggttgccg atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag
     6661 ttgcccgctg agcgccaact ggcgatgcaa ctcggcgtat cacgtaattc actacgcgag
     6721 gcgctggcaa aactggtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt
     6781 attcgctggc gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg
     6841 atggccgatg atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc
     6901 agcaccgcat ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt
     6961 tgctttgaag caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt
     7021 catctggcga ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc
     7081 ttcgatgtcc tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg
     7141 gttttttcac aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat
     7201 gccgacgggg cgcgtaaagc aatgatggcg caccttagtt ttgttcacac caccatgaaa
     7261 cgattcgatg aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat
     7321 gagcattcga gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag
     7381 cgcaacgcat tctgccgccg ttcctgttcc actatatgga tggtggtgca tattctgaat
     7441 acacgctgcg ccgcaacgtg gaagatttgt cagaagtggc actgcgccag cgtattctga
     7501 aaaacatgtc cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg
     7561 tagcactggc accggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag
     7621 ccaaagcggc ggacgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga
     7681 ttgaagaagt cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg
     7741 atcgcggctt tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg
     7801 ttttcaccgt ggatatgccg acaccgggcg cacgctaccg tgatgcgcat tcaggtatga
     7861 gcggcccaaa tgcggcaatg cgccgctact tgcaggcggt gacgcatccg caatgggcgt
     7921 gggatgtggg cctgaacggt cgtccacatg atttaggtaa tatctcagct tatctcggca
     7981 aaccgaccgg actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct
     8041 catggaaaga ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga
     8101 tcctcgatcc ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt
     8161 ctaaccacgg tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta
     8221 ttgcagatgc ggtgaaaggt gatatcgcca ttctggcgga tagcggaatt cgtaacgggc
     8281 ttgatgtcgt gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc
     8341 tgtatgcgct ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa
     8401 aagagatgaa agtggcgatg acgctgactg gcgcgaaatc gatcagtgaa attacgcaag
     8461 attcgctggt gcaggggctg ggtaaagagt tgcctacggc actggctccg atggcgaaag
     8521 ggaatgcggc atagtcgttt gcgccccctc accctaaccc tctccctcag ggagagggga
     8581 ccgttcggcg ctgtatgtac tccctcactc tgaaacgaca ccgcactctt tttttctccc
     8641 tcgcccctcc ggggagggcc ggggtgaggg gaaaaggccg cacggttcac caattttctg
     8701 ctatcctgcc cccgcactaa gggggcagta tgctaaacat cgtactttac gaaccagaaa
     8761 ttccgccaaa tactggcaac atcatccgtc tttgcgctaa taccggcttt cgtctacata
     8821 tcatcgaacc gatgggattt gcctgggacg acaagcgcct gcgccgcgcg gggctggact
     8881 atcacgagtt taccgccgtt acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa
     8941 atccccagcg cctgtttgcc ctcaccacga aaggtacgcc tgctcacagc gccgtaagct
     9001 atcaggatgg cgattatctg atgttcggcc cggaaacacg cggcctgcca gcgagcattc
     9061 ttgatgccct gcccgctgaa caaaaaattc gcattccgat ggtgccggac agccgcagca
     9121 tgaatctgtc caatgcggtg tcggtagtgg tgtatgaagc ctggcggcag ctgggatatc
     9181 cgggagcgtt gttgagagat tagttactgt atgccggata gatgattaca tcgcatccgg
     9241 cacgatcaca ggacattaga tcccatcccc atactcaaat gtatggttaa taccgttgaa
     9301 atgctggtcc atatccattg atggcttatc gctgtctggt ttaccgacaa tacgagccgg
     9361 aacgccagcg gcggttgtat gcggcggcac cggttgcagc accacggaac ctgcgccaat
     9421 cttcgcgccg cgaccaactt caatattgcc gaggattttc gcgcccgcgc caatcatcac
     9481 accttcacga attttcgggt gacggtcacc accagattta cccgtaccgc caagcgtcac
     9541 agattgcaga atcgatacgt cgttttcaat taccgccgtt tcacccacaa cgatgcccgt
     9601 ggcgtggtcg agcataatgc cacggccaat ttttgctgcc gggtgaatat cgacctggaa
     9661 cgtcacagaa acctggtttt gcagaaagat tgccaatgcg cgacgcccct gattccacaa
     9721 ccagtgaccg atgcgatagg cctgcaaggc atgaaaaccc ttcaggtata acaacggggt
     9781 tgagtattta tcgactgccg ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga
     9841 ggcgatcatt tccgggtcag cggcgtaggc ttcttcaacc acttcacgga tagcaatagc
     9901 aggcataatt ggcgatgaca gcttgttcgc cagcatgtag ctcagtgcac tgccaaggtt
     9961 ttcgtgcttg agtagcgtcg cgtggtaaaa actggccagc attggctcac agtccgccag
    10021 cgttctggct tcggctttaa tattgttcca gacaatttcc agttcttcac acgacattgc
    10081 ttactccaca cgatgagata atgaccggcc cgttctgcgc gggccgggtc atagcggtaa
    10141 caaaggttcc ctggggttag tggctgctgc gctcgtcctt gcgtgcacga cctaataatg
    10201 tcaatgctgc ctcgcgcgcg ttttttccgc aatataatac ttgataaatt tcctcggtta
    10261 ttggcatttc aacgccgaag cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc
    10321 cttccaccac ctgaccaatc ttctcctgcg cgctttgtac atccatgccc tgaccgagca
    10381 tcatgccaaa acggcggtta cgcgactggt tgtcggtaca ggtaagcacc agatcgccca
    10441 gccccgccat gcccataaag gtggcaggat cggcacccag cgccgcacca agacgcgaca
    10501 tttcagccag cccacgggtg atcagcgccg tacgcgcatt cgcaccaaaa ccgataccgt
    10561 cagacatccc cgcgccaatg gcaatgacgt ttttcaccgc gccgccaagc tgcacgccaa
    10621 tgaaatccgg gttgctgtaa acgcggaaac ttttgccgca gtgcagcagc tgctggagat
    10681 catcggcaaa ggtctgatcg gtcgaggcca gcgaaatagc tgtcggtaaa cctgccgcca
    10741 gttctttcgc aaacgttggg ccagagataa ccgccagcgg aatttgatcg cctaaggcct
    10801 cacgcgccac gtcctgtaac agacgtccgg tttccgcttc cagccctttg gtcgcccaca
    10861 ccagacgcgc atcaggacgc atcagcggtt taatctggcg cagcacttca ccaaagacat
    10921 ggctgggtac gacgacgaga atattacgac tggctgccag cgcagtggcg agatcgcttt
    10981 caagatggag cgtatcggga aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc
    11041 gttcaagcgt tgcgatatgt tcagggtcat ggccccagag gacaacctcg tggccatttc
    11101 ttgccagggt aatggcaaga gcggtgccgt acgagccggc accgatcaca gtcattgaag
    11161 cattacgttg gttcatcagg catcctgatg ttcttcagta ccttcgccag cctgctgctg
    11221 caaatagttc atgaacagcg catcgaagtt aaccggcgca aggttcagtt gcgggaatgt
    11281 accgcgggat accatgctgg tgatgcactc acgagcatac gggaacagaa tgttcgggca
    11341 gtatgcgccc aggcaatgtg ccatctgggt gccttcgata cccgcgatgg agaaaatacc
    11401 gccctgctga acttcacaca ggaacgcggt ttcttcgccc aaggaggccg ttacggtaac
    11461 acgcagtacc acttcgtata cgtcatctgc cagttgggaa gatgccgtat ccagatcaag
    11521 tttaacttct ggttgccaat ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga
    11581 gatatccttg gtataaatac gttggatctg gaaagtcatt tcagtgttgt tttgttctga
    11641 catgtgtaga aaacccttaa gtgttgtcct taaatactgc gcaatgtaat gccaacgttc
    11701 acacgttatt tcagcagggg atccagtcca ccacgtgcat ccaatgcata caagtcatca
    11761 cagccgccaa tgtgctgtgc gtcaataaaa atctggggaa ccgtggtgcg accgctgcgt
    11821 ttgatcatct cttcacgctt ggcggcattg ccatcgatcg gcaactcctg gaaactcacg
    11881 cccttgctgc tcagcagtgc ttttgcacga tggcaatacg ggcaggtttc tttggtatag
    11941 atttcaacat tggccataat ttagctcctg tttttttacc ctgaaatttc atgttgcagg
    12001 gcggctgcaa aatggagtgt ccccagaagc ttataacaat aagtcgctgg gggcctaatt
    12061 gcagctaacg gcctgcatca tgaaagacga caggtaaatt atttgccgcg caccagaggc
    12121 aagttttcgc cagcccagcc agcaacgccc tctttcagca cgaatacttg cgcaaaacca
    12181 gctttcgtca gcgcgtttgc aggctcctgg cactgcatgc cagaaccgtc taccacgata
    12241 accgatttgt ctttgtgctt ctcaagctca ccaacattgt tggctttgat ttcgctcggc
    12301 aacagattga tagaacctgc gatatggcct ttacggaagt catcacgctg acgtaaatcc
    12361 acaaccacag cgtcttcttt gttgatcaga cgcgtagctt caccacgagt aatcaccttc
    12421 actttcgagg tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag
    12481 gcgatactca gtatgggatg acggccaaca aattgcataa tttcttgcat ggggggtaac
    12541 aactcccaac gtagtgatta aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa
    12601 tacagccagc gcgttaactg gaatgcaatt ttgcggggca cgacgaaaaa aaagcgcaaa
    12661 tctggcatcg gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga
    12721 ttcagctgta gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg
    12781 gtttctaaaa aacctatggt actggtgatt ctggatggct atggctatcg tgaagaacag
    12841 caggataacg ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt
    12901 ccgcataccc tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt
    12961 aactccgaag taggccacgt taacctgggt gccggccgca tcgtgtatca ggacctgact
    13021 cgtctggacg ttgaaatcaa agatcgcgct ttctttgcta atccagtgct ggctggcgcg
    13081 gtacataaag cgaaaaacgc aggtaaagca gtacacatta tgggtctgct ctccgcaggc
    13141 ggcgtacaca gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc
    13201 gcagaaaaaa tctacctgca cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct
    13261 gaatcctcgc tgaaaaaatt tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg
    13321 tccatcattg gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa
    13381 gcctatgacc tgctgactct ggcgcagggt gagttccagg ccgatactgc cgttgctggt
    13441 ttgcaggcag cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt
    13501 gcagaaggtc agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc
    13561 cgtgctgacc gcgcgcgtga aatcactcgt gcattcgtga acgctgattt cgatggcttc
    13621 gcgcgtaaga aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc
    13681 aaaactgcgg ttgcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg
    13741 aaaaacgaca aaactcagtt gcgtatttcc gaaaccgaaa aatatgccca cgttactttc
    13801 ttcttcaacg gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca
    13861 ccgaaagtgg ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa
    13921 ctggttgcgg ccatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc
    13981 gacatggtag gtcacaccgg ggtgatggaa gcggcggtta aagcggttga agcactggat
    14041 cactgcgtgg aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc
    14101 gctgaccacg gtaacgctga acagatgcgc gatccagcaa cgggtcaggc acacacggca
    14161 cacaccaacc tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgca
    14221 ggcggcaaac tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg
    14281 caagagatga ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag
    14341 gcgattaata ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc
    14401 tacgccagcg ttcttagcgc tggcgtattg ttgtgcgcct tttccgccca cgcggatgag
    14461 cgtgaccaac tcaaatctat tcaggccgat atcgccgcaa aagagcacgc ggtacgccaa
    14521 aagcaacaac aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct
    14581 gaagccaccc gtaagctgcg cgaaacgcaa aacacgctca atcaactcaa taaacagatt
    14641 gatgagatga acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc
    14701 ctcgccgcac aactggatgc cgcattccgt cagggcgagc ataccggtat tcagctaatt
    14761 ctcagcggtg aagaaagcca gcgtggacag cgtttacagg cttatttcgg ctatctcaac
    14821 caggcgcgac aagaaaccat tgcccagttg aagcaaacgc gtgaagaagt caccatgcag
    14881 cgtgctgaac tggaagagaa acaaagcgag caacaaacgc tgttatatga acagcgcgcc
    14941 caacaggcga aactgactca ggttctgaac gagcgtaaaa agacgctggc ggggctggaa
    15001 tcttccatcc agcaaggtca gcagcagcta agtgaactac gtgccaacga atcccgtctg
    15061 cgtaacagca ttgcccgtgc ggaagccgcg gcgaaagcgc gtgctgaacg agaagcgcgt
    15121 gaggcccagg cggttcgcga gcgccagaaa gaagcgacgc gcaaaggcac cacctacaaa
    15181 ccgaccgaaa gcgaaaaatc gctgatgtcc cgaactggtg gcctgggtgc gccgcgtggt
    15241 caggcattct ggccggttcg cgggccgacg ctgcatcgct atggcgagca gctacagggt
    15301 gaattacgct ggaaagggat ggttatcggt gcctctgaag gtactgaagt taaagcaatt
    15361 gccgacggtc gagtgattct ggctgactgg ctgcaaggtt acggtctggt ggtggtggtt
    15421 gagcacggta aaggcgacat gagtctttac ggctataatc agagcgcact ggtgagcgtt
    15481 ggttcgcagg ttcgcgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggt
    15541 cggccttcac tctatttcga aattcgccgc cagggtcagg cggtcaatcc acagccgtgg
    15601 ttgggaagat aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg
    15661 gcgctctcct cccccgtact tgctggcaaa cttgccatcg tcattgatga ttttgggtat
    15721 cgcccgcaca acgaaaacca ggtgctggcg atgccttccg ctatctccgt cgctgtatta
    15781 cccgattcac cgcacgccag agagatggcg accaaagcgc ataacagcgg tcacgaagtg
    15841 ttgattcatc tcccgatggc accgttgagt aaacagccgc tggagaaaaa tacgctacgc
    15901 ccggagatga gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc
    15961 tatgccgtgg ggatcaacaa ccacatgggc agcaagatga cctctaacct gtttggtatg
    16021 cagaaagtga tgcaggcgct ggagcgttac aatctttact tcctcgacag cgtaaccatc
    16081 ggtaataccc aggcgatgcg cgccgcgcaa ggtactggcg tgaaggtgat caaacggaag
    16141 gtgttcctcg acgattcgca gaacgaagcg gacatccgtg tgcaatttaa tcgcgcaatt
    16201 gatctggcgc gtcgcaacgg ttcaaccatt gccattgggc atcctcaccc ttcaacggta
    16261 cgcgtgctac aacagatggt ttataacctg ccgccagaca ttacgctggt gaaagccagc
    16321 agcttgctga atgaaccgca ggttgatact tctacaccgc cgaaaaacgc tgtgcctgat
    16381 gcaccgcgta atccgttccg tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct
    16441 gtttatgcta atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc
    16501 gtttacttcc agcatcagtg gcaaggctgg ggcaaacagc acgaagcggc gaagtttaac
    16561 gctagcgcaa attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc
    16621 attgccataa gcggaaagac cacagtaacg cctggtaacc gaccttcacg ctgcgcacat
    16681 tggtaatcag gcgcttatac ataccggaag taaatatctc ggcaatcatc cgttggcggg
    16741 taagaatatc cggctctttg cgcaccgcat ggcaaacacg caatgcttcg taagttattt
    16801 gctgatgaaa ttccggataa atcgtaattt tgtcggcata atttcgattt aatttctcca
    16861 gcaggcgggt aatcttaata tagtgacgtt gataattaag gtttttattc ccttgtctat
    16921 gcaaccgact cactgacgta ttatgcagat aatatttata taatgattgc tcggtatatc
    16981 gcgcacgcag cgcgttaaac atgaattctg ttgtccagac aatatcctga tgatgtaatc
    17041 cggcaataaa tttaatgtta tttttaacaa taacatcacg gcgataaacc cccatccaga
    17101 caacgtgagt ccaacggcgc gaagaaagcc ccatccgcag ccagtccggg ccggttaata
    17161 caccggttga gcgaaggcga tcgctgggga tggattgcca ggtttctccc gtttcacgaa
    17221 aagaccagtc agcgttgcac tgcgccacgt cgaggtcgtc ctctaacgcc atggtcatca
    17281 gcgtttcgta catggtggga tagacctcat cgtcagcatc gacaaaagcg acatatttgc
    17341 ccgtcgccac ctcgatccca cgattacgcg ctaccgatgc gccagcattc gcctgatgca
    17401 acaaacgaac gtgcggatag ttttctgcgt aatgctttgc tatttcaaca gaattatccg
    17461 ttgaaccatc gttaataata atgatttcca gagcagtcca ggtttgcgta attaaagatt
    17521 ccatacaagt gcggaaatca tcgcccgcat tatataacgg aataataaca ctaagtttat
    17581 tggtgctgtt catcataaat taccgacgac ctctgaagtt atcctgacgt tttacaggct
    17641 ttgtattaag aaactattaa gcctgtgaga tattattttt acaggtgaga gagtcacttc
    17701 agggtagaga gataatgaga acagctatat aaaagatcag cgcaattacg aactctttat
    17761 tacactgaca ggattttaca taattaacag attatcctca gataaaaggg ctggaatacc
    17821 agcccttgtt cgtgttaatc ccagctcaga ataactttcc cggactggcc cgaacgcata
    17881 gcgtcaaagc ccttctggaa atcatcgata gagaaacgat gggtaatgat cggcgagaga
    17941 tcgaggccag actgaatcag cgccgccatc ttgtaccaag tttcaaacat ctcacgaccg
    18001 taaatgcctt taatgaacaa gcctttaaag atcactttgg tccagtcgat agacatatca
    18061 gacggtggaa tacccagcat cgcaatacga ccgccgtggt tcatggtatc gagcatggta
    18121 cgaaacgctg gcggcgcgcc ggacatttcc agaccgacat caaagccttc ggtcatgccc
    18181 agttcagcca tcacatcatt aaggttttct ttcgcgacgt taaccgcacg ggtgataccc
    18241 attttgcgcg ccagctcaag gcggtattcg ttaacatcgg tgatcaccac attgcgtgca
    18301 ccaacgtgtt tcgccaccgc cgcagccata atacctatcg gacctgcacc agaaaccagc
    18361 acatcttcgc ccaccagatc gaacgacagc gcggtatgca cggcgttacc gaagggatca
    18421 aaaatggaag ccaggtcgtc ggagatattg tcggggattt tgaaagcgtt gaacgccgga
    18481 atcaccagat attcggcgaa gcagcccgga cggttaacac cgacaccaat ggtgttgcgg
    18541 cacagatggg tacgaccgcc acgacagttg cgacagtgac cgcaggtgat atggccttcg
    18601 ccagaaacgc gatcgccgat tttgaagcct ttcacttcct gaccaatacc taccacttca
    18661 ccgacatatt catggcccac gaccatcggc accggaatgg ttttttgcga ccactcatcc
    18721 cagttataga tgtgaacgtc agtcccgcag atggctgttt tacggatttt aatcagcaga
    18781 tcgttatgcc cgagttccgg tacagggacg tcggtcatcc agatgccctc ttccgctttc
    18841 agtttggata acgctttcat ctcacatcct caggcgataa cgcccagttg tttaccaata
    18901 cgcgtgaatg cttctactgc acgcgtaatt tgctcagggg tatgcgccgc agacatctgg
    18961 gtacgaatac gcgcctgacc tttcggaacg accggataga agaaaccggt gacgtagatg
    19021 ccctcttttt gcagctctcg ggcaaatttc tgcgccacta ccgcatcacc aagcatgacc
    19081 ggaataatgg cgtgatcggc tcccgccagg gtaaagcccg ccgccgacat ttgctcacgg
    19141 aactgacgcg cgttcgccca cagacggtca cgcaactcgc tacccgcttc aaccatctcc
    19201 agcactttga tggaagcggc gacaatcgct ggtgccagcg agttggagaa caggtacgga
    19261 cgagaacgct ggcgcagcca ctcaaccact tctttgcgcg ccgcggtata accaccagaa
    19321 gccccgccca gcgctttacc gagcgtgccg gtgatgatgt cgacacgccc catcacatcg
    19381 cagtattcat gggaaccacg accattttca ccgacaaaac cgaccgcgtg ggagtcgtct
    19441 accatcacca gggcatcgta tttatctgcc agatcgcaaa cgcccttcag attggcaatc
    19501 acgccgtcca tcgagaacac accatcggtg gcaatcagca catgacgcgc accggcttca
    19561 cgcgcttctt tcagacgagc ttcaagctcc tgcatatcgt tgttggcata gcgatagcgt
    19621 ttagctttac acagacgcac accatcaata atagacgcgt ggttcagtgc gtcggagata
    19681 atggcgtctt ccgcgcccag cagcgtttca aacaggccac cgttagcatc aaagcaggaa
    19741 gagtagagaa tcgcatcttc catccccagg aatgccgcca gtttttgttc aagctcttta
    19801 tggctgtcct gagtgccgca aataaaacgc accgaagcca tgccgaaacc gtgagaatcc
    19861 attcccgcct ttgctgccgc aatcagatcc ggatgattcg ccagcccgag atagttgttg
    19921 gcacaaaagt taatgacgtg gcttccatca gccacggtga tatctgcttg ctgcgcagac
    19981 gtaataatgc gctcttcttt aaacaaccct tccgcccgtg cggtttccag atcgttggtt
    20041 aactgctgat aaaattctcc acgcattgcg attctccaga cagggcaaat tccagcacat
    20101 attacccaaa cttataggtc gcgacgagat gatgcgttaa cacttctgca aaattcagga
    20161 taaataacgt gtatcttgat gtctctcaac acctacagat agtagaaaaa ctgtacagtt
    20221 ttggctgagc ataatgatga gtgattttac tgcattgata taaaaagaaa aaatgatgcc
    20281 aaagcatcat ttttcctatc tcagttggca tcaaaacgct acccactaat ggagggaaac
    20341 ctattttctt gaaaggaaaa ttctcagcgc ctgcattttt gcccctaaag tgttactcct
    20401 gaagcagcga aacaaatcga accattttcc tctccccaga tagtttaggt tgaagagatc
    20461 ggaacgtttg taatagcaca tgacaaaaat gccctggtca tcgtccacaa tgttattgtt
    20521 tagtgttatt ttttgacttt ccagaactaa tttatagaat tctttccatt tatgctgtga
    20581 accaacaatt gcgcccccaa taatatatac atgattgcca atcatgaaat caaaagcctg
    20641 ttgttgagat gtaacagtaa gccctttttt tatagtgaaa agatgcatct tattttcatc
    20701 aaatgggaaa tcccaaattt tcaatcctcg agttacattt ggcttacggc aatagccaaa
    20761 atctatccac gcaacaagag gcgttttcac taaacccatg ttgatagctt tatttacaaa
    20821 ataggactta aggttacata ccaaaacata ctctggtgac cagtactctg ggttttttaa
    20881 ttgtcgaggc tcaagtctat ttgtaaacga ctcatctttt tgaatttttt cgatacgact
    20941 tctgatatat ctaaatttct tttttatatc gataacgata accgttgttg gttttccgtt
    21001 gcgaatcgct tcaatccttg gtttcaaatc gggagaagtg aaaataatca tctcattttc
    21061 aagtgcggcc agacgctcaa agtaactaaa ataaacatca actgaacgag ctagtttttc
    21121 acggaaccct ttattagtag tccaatcccc tctaccaatg tcaaaatatg cagtgataat
    21181 tgtggtggat gatttcatta ctaataagac cattaatata aaaaaagaat tatatcataa
    21241 atattaacag aatgtatact gtatattatc cattcaaatg attccgttat ttatcataca
    21301 tcggaatatt gatactaaag cactattcac atgcaaaacc aacatccgcc atgaaggact
    21361 agctaaaacc caaactagtt tgttgcaatt agcatccttg cacctctatg taaagggctg
    21421 aagggattcg gatgtgatgg tatgattaca gacattcgtg tctgagattg tctctgactc
    21481 cataattcga aggttacagt tatgatcatc gttaccggcg gcgcgggctt tatcggcagc
    21541 aacatcgtta aggccctgaa tgataaaggc atcaccgata ttctggtggt ggacaacctg
    21601 aaagacggca ccaagtttgt gaacctggtg gatctggata tcgcggacta tatggataag
    21661 gaagacttcc tgatccagat tatggctggc gaagagttcg gtgatgtcga agcgattttc
    21721 cacgaaggcg catgctcttc caccaccgag tgggatggca agtatatgat ggataacaac
    21781 tatcaatact ccaaagagct gctgcactac tgtctggagc gcgaaatccc gttcctgtat
    21841 gcctcttccg cagccaccta cggcggacgc acctccgact ttattgaatc ccgcgagtac
    21901 gaaaaaccgt tgaatgtcta cggttactca aaattcctgt ttgatgaata tgttcgtcaa
    21961 atcctgccag aagcgaactc gcagattgtt ggcttccgct atttcaacgt ttatggaccg
    22021 cgtgaaggcc ataaaggcag catggcgagc gtcgctttcc atctcaacac ccagcttaat
    22081 aacggtgaat caccgaagct gttcgaaggt agcgagaact tcaaacgtga cttcgtctat
    22141 gtgggcgacg tggcagatgt aaacctgtgg ttcctggaaa atggcgtttc cggtatcttc
    22201 aacctcggca ccggtcgtgc agaatccttc caggcagtag cagatgctac gctggcttat
    22261 cacaagaaag gccagatcga atacattccg ttcccggata agctgaaagg ccgctaccag
    22321 gcgttcactc aggcagatct gacaaatctg cgcgcggcgg gttacgacaa accgttcaaa
    22381 accgttgctg aaggtgtaac ggaatacatg gcctggctga atcgcgacgc ataagagctc
    22441 tgcatgaaaa tactggtgat cggcccgtct tgggttggcg acatgatgat gtcgcaaagt
    22501 ctctatcgca cgctccaggc gcgctatccc caggcgataa tcgatgtgat ggcaccggca
    22561 tggtgccgtc cattattatc gcggatgccg gaagttaacg aagctatccc tatgcctctc
    22621 ggtcacggag cgctggaaat cggcgaacgc cgcaaactgg gtcatagcct gcgtgaaaag
    22681 cgctacgacc gcgcctacgt cttaccaaac tccttcaaat ctgcattagt gcctttcttc
    22741 gcgggtattc ctcatcgcac cggctggcgc ggcgagatgc gctacggttt actcaacgat
    22801 gtacgcgtgc tcgataaaga agcctggccg ctaatggtgg aacgctatgt cgcgctggcc
    22861 tatgacaaag gcattatgcg taccgcacaa gatctgccgc agccattgtt atggccgcag
    22921 ttgcaggtga gcgaaggtga aaaatcatat acctgtaatc aattttcgct ttcatcagaa
    22981 cgtccgatga ttggcttttg cccgggtgcg gagtttggtc cggcaaaacg ctggccacac
    23041 taccactatg cggaactggc aaagcagctg attgatgaag gttatcaggt ggttctgttt
    23101 ggctctgcga aagatcatga agcgggcaat gagattcttg ccgctttgaa taccgagcag
    23161 caggcatggt gtcggaacct ggcgggggaa acacagcttg atcaagcggt tatcctgatt
    23221 gcagcctgta aagccattgt cactaacgat tctggcctga tgcacgttgc ggcggcgctc
    23281 aatcgtccgc tggttgccct gtatggtcca agtagcccgg acttcacacc gccgctatcc
    23341 cataaagcgc gcgtgatccg tctgattacc ggctatcaca aagtgcgtaa aggtgacgct
    23401 gcggagggtt atcaccagag cttgatcgac attactcccc agcgcgtact ggaagaactc
    23461 aacgcgctat tgttacaaga ggaagcctga cggatgcggg ttttgatcgt taaaacatcg
    23521 tcgatgggcg atgttctcca tacgttgccc gcactcactg atgcccagca ggcaatccca
    23581 gggattaagt ttgactgggt ggtggaagaa gggttcgcac agattccttc ctggcacgct
    23641 gccgttgagc gagttattcc tgtggcaata cgtcgctggc gtaaagcctg gttctcggcc
    23701 cccataaaag ctgaacgcaa agcgtttcgt gaagcgctac aagcagagaa ctatgacgca
    23761 gttatcgacg ctcaggggct ggtaaaaagc gcggcactgg tgacacgtct ggcgcatggc
    23821 gtaaagcatg gattggactg gcaaaccgct cgcgaacctt tagccagcct gttttacaat
    23881 tgtaagcatc atattgcaaa acagcagcac gccgtagaac gcacccgcga actgtttgcc
    23941 aaaagtttgg gctatagcaa accgcaaacc cagggcgatt atgctatcgc acagcatttt
    24001 ctgacgaacc tgcctacaga tgctggcgaa tatgccgtat ttcttcatgc gacgacccgt
    24061 gatgataaac actggccgga agaacactgg cgagaattga ttggtttact ggctgattca
    24121 ggaatacgga ttaaacttcc gtggggcgcg ccgcatgagg aagaacgggc gaaacgactg
    24181 gcggaaggat ttgcttatgt tgaagtattg ccgaagatga gtctggaagg cgttgcccgc
    24241 gtgctggccg gggctaaatt tgtagtgtcg gtggatacgg ggttaagcca tttaacggcg
    24301 gcactggata gacccaatat cacggtttat ggaccaaccg atccgggatt aattggtggg
    24361 tatgggaaga atcaagtaga gtgtcgttct acaagtatgt ctcttgcaga tttgccagct
    24421 caaaccgttt ttcaaaattt aaatcttgaa ataataacca ataagttgac atcggagata
    24481 agatgacctc aacattattt ttctctctcg agaaaaaaaa ctggatagcg tactggaaca
    24541 gagctctcgt attcttattc attaccacct attttttggg tgggattaca aggtataaac
    24601 atcttattgt tattcttatg acaataacga caatcgtcta tctctgcaaa cggccaaaac
    24661 actatctctc actatttaaa acatttcttt ttggtagtgt tgccatatta actattgctg
    24721 cattgctgtc acttcttcaa tcccctgatg caggtgctag catgaaggaa attttcaaag
    24781 ctattattga gaatacttta ctatgcacaa tagcaatacc ggtcatattg agagacgaga
    24841 aaagagaaga tgtcgaaaaa atcgttttct tctcatttat tagtgcgttg ggcttacgct
    24901 gtttttctga attgattacc tattataagg actatcaaca agggataatg ccattcgcag
    24961 attatagaca ccgtagcatt tctgactcga tggtcttttt attccctgca ttgttaaatc
    25021 tctggcttat caaatcagca aaataccgca tttcttttgt ggttctaagc gttattttta
    25081 tttttctgat attaggaact ttatccagag gggcctggct ttccgtgtta gtcattggat
    25141 taatatggat tctgatgttt aaacaatgga agttactatt agtaggagta atggtttcca
    25201 tcattgcatt gtcggttatt ttcacacata aggagatgac cgcaaagcta acgtataaac
    25261 ttcaacaaac taatagttct tatcgctatg caaatggtac tcaaggcagc gcactcgatc
    25321 taatattaga aaatcctgtt attggttatg gttacggtaa cgttgcatat aaagatgtct
    25381 ataataaacg tgtcattgat tatccagaat ggacctttag acaatcaata gggccacata
    25441 attttgcgct attcatctgg tttggcactg gtttattagg gctggtaagt cttatgatgc
    25501 tatactgtgc aatattgaaa gagtgtataa aaaatggcgt caagaataaa tatcgctcac
    25561 catataatgc atattatata atcttactat cttttatagg ttattttgtt atccgtggaa
    25621 acgtagaaca aattgaacca aatttattag gcgtttacgc cggcttatta ttagcgatga
    25681 aaaacaagta aaaaataaaa aaggctgcat aatgcggcct ttttatttat tcaaaccaat
    25741 tatgaataac ctcttcgaat ctttgagtta cgccatccca actgtagtga tcaaacacaa
    25801 agtcctgacc ttgctttgca actgctgtta attcaggatt cgctaacgtt tttaaaatat
    25861 cgctgcttat ggagtctgca gtcataggtt cttttaaatg gaatcccgta gtattttctt
    25921 tgacaaattc cgtcatcccc ccacgcgtac tcactaaaac aggcttacca gcacccatag
    25981 cttcaattgc caccatgcaa aatggttcct ggaactggga aggaatgacg actaaatctg
    26041 ccagtgggta ataacaatgc atcttttcag gcggtatgct tcctaacata atgcaacgat
    26101 ccttcaaacg ttttgcaatt tccctgacat tgcgttgata tgcacctttg tcacttttac
    26161 tcatttctgt ataatcacca atcacaacta attttaaatt tttgtgagca gcggccaatt
    26221 tttcgaatga ttgcatcaac agcaatatgc ctttgtcagg gacgatacgc ccagcataaa
    26281 aaataatttt ctcttcaggg gagatattaa gttcgcttct agtgatcggc tgaaaattag
    26341 attgataggt ttctaaatca attccatttg gtacaatctc aatatctgca tttgctaaat
    26401 aagattggta atactttttc aagtataaac tagggactat cattttaaca ttttgctcaa
    26461 gcaattttgg ttcaaatgca ttatgcatat gtataaccac tcttgcctga ggagctcgct
    26521 tacgaatttg agtgtatagc ttcatgctat tatgaactac aataacactt tctttcgtta
    26581 taggaaaatc atgtgcaata ttcaatattc tctgtgaata tggaagagga tcaagtcttg
    26641 tccatttttg aaataaccgc ttatatatgc gactgaatcc aaccctatga atactacaat
    26701 tatcattaac aaaggtgtaa tttgagtatc ctggattttt gatacaaaca atacgatttg
    26761 gaaaattggt acgctgagca acctgataca tccacgtttc aacagcagcc gctccgcgag
    26821 gaggaattga aaaaatagga gtaaccgtaa atattatttt atcaaccatc tcgtatatta
    26881 acctttcata acattatatt taattgctgt aaaaacacca tcgagaaatt ttttctggta
    26941 aagcaagtgt ttatattttt ctttgtgctc atgtttttta acagcttttt tataaggtat
    27001 atttctccat ggtgatatat tataaatatt acgaaaataa tctgcagagg cgtagttcgc
    27061 ccaatcatgc catggcttag ttataccagt ataatgtatg aagacagtgt catcattaat
    27121 aaaccgggtg taatattcac tatttttttc ttcaaattct gactttatag tataaataca
    27181 attatatttt ctcggtaata ttttagcatg atgtagaaac attaagttta aaatatcttg
    27241 atccgggtac tttaattttt ttataattga ctcatctgat aatagatcaa agaatttttc
    27301 cgttagtctt tgttttaacc attcccgtaa attaatatac attacaccag agttaaaata
    27361 gctaccgtta aaatcctcat tacacaaacg ctcagcgctt ttactttgca tagcatctac
    27421 atcaattacc actgccccat attcatcttt aaactctaat gctattaact cgttcaatga
    27481 acccttacag acgatatcag catcaagata taataatgaa tccaatcgcg cagaaaaata
    27541 atcaaatgaa agcaaacgat aataaataga tactggccag atatttgatt gaggtagcgc
    27601 cttcaatggc tcagaatcaa tatgatataa tttaattact gtttgatact tcgttgctaa
    27661 ttgtgataat aattcaatat actctttgtc taagtaatca gcgattatgt gaaaggtaaa
    27721 tttgttttgt ttattctttt caagaattga agcaattgaa atcgcagccc caacctggta
    27781 gttctcatca ataccccatg agacattaaa cgtttcccgt tcatcaagtt taacggggcg
    27841 cttgtccagt tcaattatat ctttaaattg agtaagatgt ttaaaatcca atttaattac
    27901 ccttcatcat aatcattatt tgctcttcaa tcctagtttg acttttacat ccctgattac
    27961 ttttttaatt ttctttttga atataagata tgtatagcca aagtctttca attcattttt
    28021 tatattatag tgccgttcaa gatcaatgcg atcttttgct tttaaaacag cggttgtttt
    28081 tttacctgaa aggtcaatta gtcttaaacc tttttcggat actataaaat ttcctttatg
    28141 aggatcgcca gacaccattc catgctgatg caactctttt atcgattctg ataattgatc
    28201 cttcagatct tcagaaattt ccaaatactc atttaacccg acaccttcaa tatactcaat
    28261 gagcataata taataatgag cgaaatttaa tgttttacgt tcagctagca aaaaatagtc
    28321 attaattgat tggatgcctt caccacgtac ccgatcggtt tgataaatta aattttcata
    28381 ataatctttt tttatgcaag attttaagaa acgttcggtc attttatgct ttggcgcata
    28441 aactttaagt acaagaggtc cacgagcagt gtcaataaga atgactttag tatcatcaat
    28501 agaacgaaaa acttttaatg ttttaaccct gcaagttaaa aagtcattta gaacttgctc
    28561 ataaaaggga tcattatctt ttatgaaaac tttcaaccca tttatcgttt tattatatat
    28621 cataatgaat tattttaacc ttaaatcttt agaagcattt ttctttataa tactttaaat
    28681 aattaaaaat gcctgccata taatgctttt gtttaaattt atgttttgca caataccgat
    28741 actgattgct atttacaggt ttaagtaaat cttctttact ccacggagaa gctgctttgg
    28801 cgatcaaaaa actacgtgaa accggataat cagcccactc atgccagggt tttgtaggtc
    28861 ccacatagtg tataaacacg gtgtcatcat tgactggatt atcaactttg tcttttaatt
    28921 catagttaat actatatcgg gtattatatt tctccgaaat aaacttcact ttaccattca
    28981 ataatacatt cagtacatct tgatcaaggt gggtgatttt acttacccaa tctgggtcac
    29041 gcaacatttc aatagctttt gacgaaatgt tatttagatt ccactcatca atatttatca
    29101 gtaaaaaacc agcattgaaa tatccagaag ctaactgtgg tgtagttaat accgaggctc
    29161 gattttgcca ccattcaata tctctttcag ctacaactgc ggcaatttca ttagtagaaa
    29221 attgataatc taagagttct ttaatactac ccttgcaagc aatatctgca tcaagatata
    29281 gtattttttc atgtttatga tagaaataat ctgcaattat aaatcgaaaa tatgttgcgt
    29341 aagtccagtt tttcgtgctt ggtaatgact tcagcttatc acaattgata acataaatat
    29401 taattcttga attatattgt tttgctaaat cagaaaaata taatttgtct ttatcactga
    29461 tataatctgt gaaaacatga aattcacaag agatttctct attgtttaat agaatagatg
    29521 cgatggctac accacatcca aaaagaaaat ttctgtcaat accaaatgct atatcaagag
    29581 tatttttaga tcttatattt tgataatgaa aattatactc aaaaaggatg atgtcactat
    29641 cattgagttg agacatattt attctgtatc caaaattttg gcaaaaataa gttacgggtt
    29701 acaacgattt tcgaatcgtt ctttccctga tttttgtggc ttttgcttct gcttgcgaca
    29761 gcagtccttg ttcctgcttg agaatgtctt taagcggggc ggcaaaatac accttcataa
    29821 agcgccagat atcccgctga gtcaggccaa tattcattga agaaaaataa agccctataa
    29881 gatctttatc gcgccaacga cctggaacgc gcgtgcgaag ctgcgcccgg tgtaggtcaa
    29941 ttaccgaaat ttttaactcc tcttccttac cggaaaaagg caagtgcagc aggaaatgac
    30001 agatataaca gtcacgatgg ttaatgcccg cagcatgcat atcgcgcacc atcgtcgcga
    30061 cacgcttaat aagcatacgc tttacgcgaa catctggtgg gttggtcgcc cagtcagcac
    30121 agtaatcttc cagactgatg gttggtgtca gatcttcggt aataataaac gaggttctgg
    30181 tcagcggatt catgcctttt tcgccgaatg ccaccccata catagtatca acgccgacat
    30241 cccgcagtcg atgaatcgca ttccattcgc ggtctgcgcc taatactggc atccgcaatg
    30301 agagtaaatt tttgattatc tctttcaggg tcgtgccgcg atgccatttg agaaaatagc
    30361 tttttcccgc catttcaaag cgcagagtac ggcgagtttc cagttcacga aatacctcac
    30421 cctgcaaggt tttaacttcc tcaaaaggat ctttgccgcg ccataacgtg gcaaacggct
    30481 ctttaagttc aaccatccag accacccgtt atgatatccg ccgctttctc tggcagactg
    30541 tataaatctt gtgtatcagc ataatgtcgc gcattttccg cccaagcctg gcgcaatgaa
    30601 gattgcgtta acgctttgcg taaaatctca ttcaatgttt cctggcggaa tggctcagca
    30661 atagcctcgc cgcaattagc gtcgacaata taatgcgcat agccacaaac ggcagttgtt
    30721 agtaccggta atcctgcagt aatcgcttcc agcagcacaa ttcccgccgc ttcctggtag
    30781 gcaggatgca gtaataaatc cgccgccgcc attaattccg agacatcgtt gcgccccgag
    30841 aagaagtgaa cattactgcg cacgccgcgt ttttctgcca gtgcctcaaa ttttcgcggt
    30901 ttatcctgcc caacaacata tagcaatgtg ttgtggcgca gcgaatccgg taacgaagca
    30961 agtgcttcaa tggaacgatc gacaccttta cgcgtgaagt ctgaaccgac ctgcaacaat
    31021 aaatattgtt gttcggttat tccattcttc ttacggaaga tttcacggct attggctggc
    31081 tgctggctat atttacgatc aggataaatc cccggtggca gaatatgaaa acgctccgct
    31141 tcagtctgat aatgtttctg gaaatcggcg atttgcttat ctgtcagcat cagcagctgt
    31201 gtcggcttgc cctgttcgaa ggttgcccgc tcaaaggcgg cataatggcg ataacgtgac
    31261 gtcaggcgat agaaaaagcc tttttcctgc gctactttct cggcataaca aacatcagcg
    31321 gcataataaa cgtccagccc cggcattttg ttgaatccaa cgactttatc gacgggatgt
    31381 tcgcgtaaat gtttttgcac ccaggcaaaa tactccgcat tgcgcccgtg attggtatgc
    31441 gatttaaccg gcactttgat cagttcaaat acatcagggc attcgccttc ccacgactgg
    31501 gtataaaccc gaacatgatg acctcgggcg gcgactgtct gagcaatacg cataaaatcg
    31561 cgctgcaaac cgccaaaggg aaaatattta tataaacaaa aagcaacgat cataacgacg
    31621 tacctgtcgt ggaggagggc agtaatttat cgacagcagc aatgacatct gccgccggaa
    31681 taaccgaaag atacatctca tttcggtcac gctgatcgcg cgttggcatt tcccggtaat
    31741 ctcccgccca gaattgaatc atgttatttg accagggacg ccagaaaata tgatctgttg
    31801 cgccaaacag cgatatcagc ggcgtattaa ctgcagcggc aatatgcgcc ggtgcggaat
    31861 caacgccaat aaacagctgc gcatgatcga ttaacgcacc aagttccggg aaggtcacct
    31921 ttccagccag cgccgttact ggtggcgtct ggcatccctg cgcaatttca ttgacgcagg
    31981 ccagatcgtc tttatctggg ccggacgtca gaacgacttc ataaccacga gcatgtaagg
    32041 catcaatcac agcggaaaac ttggcgttgt cccagcattt gaagatttgc cgcgccgtag
    32101 gttggataac cacataattt tgtccaacac cagcgtgatc aagttcgcga cgcatacgtt
    32161 tccagcttgc aggcgggtaa ctcatggttg tctgcttcac caacgaatca agtcccaatg
    32221 gtgtcagcac ggataagtta ctttccacca catttccacc ctgcaacggc accaaatggg
    32281 tgaaactttt acgccaaaaa gcagactgcc gatgatgata atcctgggaa attttcacac
    32341 gagcatttaa taagcgaacc agtatagcaa ccatccattg atcggtaaga ttgacgataa
    32401 ggtcatactt attggcacgt aataccttga tgagatgaaa aaagttggca attttttctg
    32461 aggcttttgc ttttttattt tttatgccgt agagcgcgtt aatctctgga ttttcagaca
    32521 ggatcgggat ggtgtcctga taaagcagca catcgatttt tgcgtcaggg taattttttt
    32581 tcagagaact aatgacggga gtagttaata acatatcccc atgaaaacgc atcttaatga
    32641 gcaaaattct tcgaaatggc ttatccacaa gtaactcttt ttgttaagat tatccgatta
    32701 aggtgaagca gaaaaagcac gctaccgccc caggctcaac agctacctga atactgatac
    32761 cgcactgata ttgacgcgct agtgtatcac ttctttcgcg gcaatccgac ccgaatgatt
    32821 tttaagcaca ttgttgattt tcaataaaga aatcaatcaa ataaaatcat tagcaaccat
    32881 attctttcac tgttgctctg cg
//
LOCUS       DAJVQH010000032        32707 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJVQH010000032 DAJVQH010000000
VERSION     DAJVQH010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.032"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..869)
                     /locus_tag="OQM87_001594"
     CDS             complement(<1..869)
                     /locus_tag="OQM87_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCS1507768.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNREGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLP"
     gene            complement(1047..1958)
                     /gene="gsiD"
                     /locus_tag="OQM87_001595"
     CDS             complement(1047..1958)
                     /gene="gsiD"
                     /locus_tag="OQM87_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCS1507769.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1961..2881)
                     /gene="gsiC"
                     /locus_tag="OQM87_001596"
     CDS             complement(1961..2881)
                     /gene="gsiC"
                     /locus_tag="OQM87_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCS1507770.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2899..4437)
                     /gene="gsiB"
                     /locus_tag="OQM87_001597"
     CDS             complement(2899..4437)
                     /gene="gsiB"
                     /locus_tag="OQM87_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCS1507771.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(4457..6328)
                     /gene="gsiA"
                     /locus_tag="OQM87_001598"
     CDS             complement(4457..6328)
                     /gene="gsiA"
                     /locus_tag="OQM87_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCS1507772.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6315..7280)
                     /gene="iaaA"
                     /locus_tag="OQM87_001599"
     CDS             complement(6315..7280)
                     /gene="iaaA"
                     /locus_tag="OQM87_001599"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1507773.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7484..8719
                     /gene="moeA"
                     /locus_tag="OQM87_001600"
     CDS             7484..8719
                     /gene="moeA"
                     /locus_tag="OQM87_001600"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCS1507774.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPISATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8719..9468
                     /gene="moeB"
                     /locus_tag="OQM87_001601"
     CDS             8719..9468
                     /gene="moeB"
                     /locus_tag="OQM87_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCS1507775.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9647..10309)
                     /gene="fsa"
                     /locus_tag="OQM87_001602"
     CDS             complement(9647..10309)
                     /gene="fsa"
                     /locus_tag="OQM87_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1507776.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10440..11339
                     /locus_tag="OQM87_001603"
     CDS             10440..11339
                     /locus_tag="OQM87_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCS1507777.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11345..13777
                     /locus_tag="OQM87_001604"
     CDS             11345..13777
                     /locus_tag="OQM87_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCS1507778.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13923..14738
                     /gene="ybiV"
                     /locus_tag="OQM87_001605"
     CDS             13923..14738
                     /gene="ybiV"
                     /locus_tag="OQM87_001605"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCS1507779.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14890..16155
                     /locus_tag="OQM87_001606"
     CDS             14890..16155
                     /locus_tag="OQM87_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCS1507780.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16396..17988)
                     /locus_tag="OQM87_001607"
     CDS             complement(16396..17988)
                     /locus_tag="OQM87_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCS1507781.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPECVIDFSGNYEDYLRSKGIE"
     gene            18207..19127
                     /gene="ldtB"
                     /locus_tag="OQM87_001608"
     CDS             18207..19127
                     /gene="ldtB"
                     /locus_tag="OQM87_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1507782.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(19185..20303)
                     /locus_tag="OQM87_001609"
     CDS             complement(19185..20303)
                     /locus_tag="OQM87_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCS1507783.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(20300..20767)
                     /gene="mntR"
                     /locus_tag="OQM87_001610"
     CDS             complement(20300..20767)
                     /gene="mntR"
                     /locus_tag="OQM87_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCS1507784.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20953..21081
                     /gene="mntS"
                     /locus_tag="OQM87_001611"
     CDS             20953..21081
                     /gene="mntS"
                     /locus_tag="OQM87_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCS1507785.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21353..22936
                     /gene="opgE"
                     /locus_tag="OQM87_001612"
     CDS             21353..22936
                     /gene="opgE"
                     /locus_tag="OQM87_001612"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1507786.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22984..23499)
                     /gene="ompX"
                     /locus_tag="OQM87_001613"
     CDS             complement(22984..23499)
                     /gene="ompX"
                     /locus_tag="OQM87_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCS1507787.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23552..23617)
                     /locus_tag="OQM87_001614"
     CDS             complement(23552..23617)
                     /locus_tag="OQM87_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507788.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23852..24739
                     /gene="rhtA"
                     /locus_tag="OQM87_001615"
     CDS             23852..24739
                     /gene="rhtA"
                     /locus_tag="OQM87_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCS1507789.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            25038..25541
                     /gene="dps"
                     /locus_tag="OQM87_001616"
                     /gene_synonym="pexB"
     CDS             25038..25541
                     /gene="dps"
                     /locus_tag="OQM87_001616"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCS1507790.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25945..26691
                     /gene="glnH"
                     /locus_tag="OQM87_001617"
     CDS             25945..26691
                     /gene="glnH"
                     /locus_tag="OQM87_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCS1507791.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            26830..27489
                     /gene="glnP"
                     /locus_tag="OQM87_001618"
     CDS             26830..27489
                     /gene="glnP"
                     /locus_tag="OQM87_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCS1507792.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27486..28208
                     /gene="glnQ"
                     /locus_tag="OQM87_001619"
     CDS             27486..28208
                     /gene="glnQ"
                     /locus_tag="OQM87_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCS1507793.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            28469..30693
                     /gene="ybiO"
                     /locus_tag="OQM87_001620"
                     /pseudo
     CDS             28469..30693
                     /gene="ybiO"
                     /locus_tag="OQM87_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(30690..31616)
                     /gene="rlmF"
                     /locus_tag="OQM87_001621"
     CDS             complement(30690..31616)
                     /gene="rlmF"
                     /locus_tag="OQM87_001621"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCS1507794.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            31892..32152
                     /locus_tag="OQM87_001622"
     CDS             31892..32152
                     /locus_tag="OQM87_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1507795.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            32417..>32707
                     /locus_tag="OQM87_001623"
     CDS             32417..>32707
                     /locus_tag="OQM87_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507796.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKN"
BASE COUNT         8022 a         8730 c         8093 g         7862 t
ORIGIN      
        1 ggtagataaa aaagtttatc tgatgcgttc aataaattac cgccaggata ataaaaatag
       61 atttcgaaca atgcattatt tggcactgct gatgtctttg caatatactc cgtatcgaca
      121 gaatcaataa tattataatt accttctcta ttataaataa tcagtttttc acgagcgagt
      181 tgattcttgg caatgatctc atgaccttgt ttcaaaaata gaacttcatc attagcgctg
      241 atttgatcaa gatactcgtt cagataccgc cagtttaaat cgacatgaat tttatctacc
      301 gctaacaaat cgtgatcttt gccgttgata cgtagcgtaa aagagataca ttttttaccc
      361 ggacgatcaa agcaattgta tgccggattc cagctgttgt tagagtcatc tgaagccacc
      421 gaccacggtc gtttcgatgg gttaaaatcg gcaaaaataa cccgccctga ctcgtcgaca
      481 agcggtccat ttacttgttc ctgatgataa acaacgattt tatcattcgc cctccgcgaa
      541 aagagataat atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt
      601 tccattttca gaatcacggc gcgttgaatc tcctctgtat tttttaataa atacagtgag
      661 tcctttaatg tcagggccga acgttcaata tctttaaaga aatagccaac gtttttctga
      721 ataagttcaa agcggctatc ggcacgtttt accgcgcgtt ccgataccag ttgatagtta
      781 atcatgaaaa aaagaccaat aaatgacagt gctgccaata aaaataaaat tatcagcctt
      841 atctttatct tttcgtataa actcagcatg ttaatacatt taacaaaata actatctgaa
      901 cgctgtgccg ctgcaaaaat agtcccttgc tgcggctttt taaccaaatt acccgattct
      961 acaaaatcag cagaaagtta caacacttgc ttagggcaat aatttcctgt gaggtgatta
     1021 ccctttcaag caatattcaa acgtaactat cctttaattt tcggatccag cgcatcgcgt
     1081 aaaccatcgc ccaacaaatt gaacgccagt acggtcagaa aaatagccag ggccggaaaa
     1141 acagcgacat gcggcgcgat aaccatatcc gctcgtgcct cattgagcat tgctccccac
     1201 tctggtgtcg gcggctgcgc acctaggccg agaaatgaca ggctggcggc agagataatc
     1261 gaggtgccaa tgcgcatggt gaaaaacacc acgatagaag agacggtccc aggcaggata
     1321 tgacgcaata aaatggtcat atcgctggca ccaatactgc gtgctgactc aataaaggtt
     1381 tgctgtttca acaccagcgt gttgccgcgc accaggcggg caaacgcggg gatggaaaaa
     1441 atggcgactg cgataatcac gttagcaatg ccgcttccca acaccgcaac aacagcgatc
     1501 gccagtaaaa tacccgggaa ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc
     1561 caccagcctt cataatatcc agccaacaag cccagcaacg tccctatcgc cacaccgata
     1621 aacacggcaa acacgcccgc cgccagcgag atttgcgcac caaccaggac acggctgaaa
     1681 atgtcgcgcc ccagtgaatc gacgccaaac cagtgctgca aagaaggtcc gttattcaga
     1741 ttgtcataat caaaataatt ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc
     1801 accacaatca ataaaataac gaataatgcg gcggtcatcg ccatatgctg acggcgaaat
     1861 cgtcgccaga attcatgcca cggtgtacgt acctggtcag gtttgaccag tggcatggcg
     1921 tttaacaccg cctgacgtcg ccagttaaat agtcgcatcc ttacttgtac ctgatagccg
     1981 ggttaatggc ggcgtaaagc acatccacca ctaagttgat aagaataaat tccagcgaga
     2041 aaagcagaat ttccgcctga atcaccgggt aatcgcgcat ttctacagag tcaacgagta
     2101 agcgtccaag tccgggccag ttgaaaactt tctccaccac gatagaacct ccgagcaaaa
     2161 agccaaactg taagcccatc atggtcacta ccgggatcat cgcgttgcgt agcccgtgtt
     2221 tgaggacaac ccaggtttcg ctcacccctt tcgccctcgc ggtacgcata taatcttcgc
     2281 ttaaaacgtc gacaaatgac gcacgggtaa agcgcgccat cacggcggcc actgccgcgc
     2341 cgagcgtcag ggagggtaaa atgtagtgct gccaactgtc tgctcccaca gtaggcagcc
     2401 agcccagttc aacggagaat acctgaatta aaaacatccc cagcgcaaat gccggaaacg
     2461 agatccccga caccgcaatg gtcatactca atcgatccgg ccaacgatta cgccagacgg
     2521 cggcgataat tcccgccgcc ataccaaata tcaccgccca gaccatactg gttatggtca
     2581 gccacagcgt tggcataaag cggctggcaa tctcatcggc aaccggacga cgcgacacca
     2641 tcgacaggcc aaaatccccc tgcacagcat tgctgatata gtgccagaac tggtgataca
     2701 gcggctgatc caaccccagc tgctgacgca ccagttctat aacctgcgca tcagcttcgg
     2761 gcccggcaat caatcgcgcc ggatcgccgg gcagcatgtg gacaaataaa aacaccagca
     2821 ccgagacgat aaacagcgtc ggaatcaacc ccagtaatcg tttgataacg taattaagca
     2881 ttccactccc tgcgttgctt attgcaaatc cgcgtcttca aagctgaagc cgctgtctgg
     2941 catgatccaa aaaccggtca ggtttttact gtgtgccgac accagttttt ctaccaccag
     3001 cgggatccac ggcgattctt gccagatgat atcctgcgcc gccttatata agcgggtctt
     3061 ttccgccgga tcattcgttt tcagtgcctg agccaggaag tcatccacct gtttattgct
     3121 gtaaaacgcg gtattaaaca gcgtcggtgg ccagttttgc gaggcaaaca gcggcgatag
     3181 tgcccagtcg gcttcgccgg ttgaagccga ccagccagtg tagaacatcc gcacgccgct
     3241 ctctttttgc cctttacctt caacttctgc cgcccgctgt ccggcatcca tcgcggtcac
     3301 ctgggcttta atcccgacct gcgctaactg ctgctgggta aattgcagca ctttctgcgc
     3361 ggtgctgtgg ttatgtgacg accacagcgt ggtactgaaa ccgttgggat atcccgcctc
     3421 tttcagtaat tcgcgcgctt tcactggatc gtaaggccac ggtttataac tttgcgcgta
     3481 ggcgatactt ggcggtacca caccagtagc tggcgttgca tagcccgcaa aggcaacttt
     3541 caccagcgcc ggacggttaa tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa
     3601 cggcttttgc gtcacgttca tactgatata acgctgcata attgacggac tggccatcaa
     3661 ctcgatattt ttgtttttct ccagcagtgt ggcctgctcg tagggaatgg ggaaagcaaa
     3721 ctgcgcttca ccggtttgca gcattgccgc gcgggtgttg ttatccgcca ccggacgcca
     3781 ggttatgctg tccagtttgg gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt
     3841 cacaaaatcg gtctgattcc aggtatccag ttcatacggt ccggttccca ccggatgaaa
     3901 accaatctcc ttgccatatt tttccagcgc tgtcggtgaa atcatcgcgg tcgccggatg
     3961 ggcaagaata ttaataaacg ctgagaacgg ctgtttgagg gtaatcttta ccgttgtcgg
     4021 atcgatcgcc tccgttttgg caatattctt atacaggtta tagcgtttaa gatgattcgc
     4081 cggatcgctg gcccggtcca gattcgcttt taccgccact gcgttgaaat cggtgccatc
     4141 ctggaattta attccttccc gcaatttcac ggtgtaagta atgccgtcat cggaaacggt
     4201 ataactctcc gccagcacgt ttttcagttt catctcttta tccagaccga acagcccctg
     4261 gtaaaacgat ttcgctacgg cctgagataa cgtgtcattc gcgtcatacg gatcgagcgt
     4321 ggtgaaattc gatcctaccg ccaccaccac atctttggca gcgaatgcac aagatgccat
     4381 caacgctgtc gcaatgccca gcgccactaa cccactacgg tgtacagctc ttgccatttt
     4441 attctccgcc tgaatgttat ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt
     4501 aatgccccgg cccgacgcat tgcaacgaga cggctgccac ctcttcgcca cgcagatgaa
     4561 tattgctggg aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg gacggttcag
     4621 cgaccggaac tgccgccagt aatttacgcg tataaggatg ctgcgggttt tcgaagaccg
     4681 cgcaccgtga accaatttca acaatttgcc cgagatacat caccgccaca cgatgactaa
     4741 tccgctctac cacagccata tcgtgggaga taaacagata cgcaatgccg aaatcacgct
     4801 ggagatcgag caacaagttg ataatctgcc cgcgaataga aacatccagc gccgaaacgg
     4861 cttcgtcggc aatgatcact tttggattca atgccaacgc gcgagcaatg cagatgcgct
     4921 ggcgctgacc gccggaaaac tcatgcgggt aacgccaggc atgttcaggt aacaggccca
     4981 cgcgctccag caaccacgca acgcgtgcag ccgcgtcttt acctggcaat aaaccgtgta
     5041 cacgcagcgg ttcgataatc gaatcaccga tggtctgacg tgggtccagc gaagcgtaag
     5101 ggtcctgaaa aataaactga atatcccggc gcaatgcctg aagtttgcca ggtgacaagg
     5161 tatcgattcg ctgaccgtta aagataattt cgccgccctg cgattcgacc aggcgcagca
     5221 acgcccgccc ggtagtggat ttaccgctgc cagactcgcc caccagcgat agcgtttcgc
     5281 caggccagag atcaaaactg actttctcaa cggcatgcac ttcccgcgtt acgcgattca
     5341 acaaaccgct gcgcaaaggg aaacgggtga caagattacg cacccgtaaa acaggttcgc
     5401 catccaccac cgttttctgc tcgatggggg gttcctgttt cgctggatgt tcaagcgata
     5461 tcaatgggaa acgtcgggga taatctaacc ctttcatcgc accaagttgc ggaacagcag
     5521 ctaacagcgc acgggtgtaa ggatgttgcg gtgcatgaaa aatctgttcg acggtacccg
     5581 tttccaccgc ctcgccctga tacatcacca gtacccgatc ggcaatctct gccaccacgc
     5641 ccatatcgtg agtgataaag ataacgccca tcgacatctc tttttgcaat actttgatta
     5701 attgcaggat ctgcgcctga atagtgacat ccagcgcggt ggttggctca tcggcaatca
     5761 gcaccgccgg gcggcatgac agcgccatcg caatcatcac tcgctggcgc atcccgccag
     5821 agagttgatg cgaataacgt gaaagaatgg tttgtgcctc cggaatgcgt acctgatcca
     5881 gcatccgctt cgcctcgacc atcgcttctt cacgactggc gttctgatgc agacgaattg
     5941 attcggcaat ctgttcaccc acagtaaata ctgggttcag cgatgtcatc ggctcctgaa
     6001 atatcatcgc catatccgca ccgcgcacat ggcgcatttg tgcagcgctc tgctcgctaa
     6061 gttcaatcac atcacgactg cgccgccgca acagcatttt atcgcactgt actaaaccgc
     6121 ccgcctgttc caacagacgc atcaatgcca gcgcagtcac tgacttaccg gagccggatt
     6181 cgcctacaat tgccagcgtc tcaccgcgtt gcagactaaa agagagattg cggaccgcag
     6241 ctattttctg ctggtcctgc ataaaggcaa tattaagatt ttcaaccgcc agcacattac
     6301 cggcatcaag ttcatcactg tgtggcaacg gtgtcccctt tttcacggta gataccggtg
     6361 gttggcgtat cgcctgcgta gccccaggcg cgatacattc cttcggtgtt aaacggtagc
     6421 gcgacattcc cttcatggtc gatagcgatt aagccaccgc taccgccaag cgcagggagt
     6481 ttttccatta ctacccgctc gcaggcttcc gcgagactta atccgccgta atccattaac
     6541 gcggcgatgt catatgccgc cagcgcgcgg atgaagactt cgcctgtgcc ggtacaagaa
     6601 accgccacac tggcgttatt ggcgtagcat ccggcaccca ctaagggact atcgccaact
     6661 cgtccgggta atttattggt cattccgcct gtggacgtgg ctgccgccag attgccgtct
     6721 aaatccaacg ccacggcccc cacggtgccc attttctgtt tttcatccag tggcgcaccg
     6781 ctatggtcga ggactgttgc cccttcctcg cgcgctgcca gtagttgttc ataacgcaaa
     6841 ggcgtggaga aaatctccgg tgagacgcgc tccatgccat gagcaaacgc aaaattttct
     6901 gccccttcgc caatcatcat cacatgcggg ctttgctcca tcaccagccg ggcggcaaga
     6961 accggattac gcagatgact aacgcccgcc accgcaccgg ctttcagggt gttaccatcc
     7021 atcacacagg cgtccagttc atgggtttca tcacgcgtaa agacagcgcc aattccggcg
     7081 ttaaacagtg gacactcttc cagcagacgc accgcttccg tcaccacatc cagcgcactt
     7141 tcgccggctt ccagcatttt ctgcccggtt tcaacaatgg cagacaacgc ctcgatgtag
     7201 cgtaattcct gttgcagact catctgcgcg cggctaattg cacctgcgcc accatgaatt
     7261 gcaatgactg ctttgcccat agtattcgtc cattaagggt ggctcgccac ttttgctata
     7321 aatatcagaa tttttatgaa ttactcattc aatttttgag tatagaaaga tggatatggc
     7381 atgtaaaggc aggagccaac gctacgcccg aggtggcata cagtctatgg cgtttttctg
     7441 acataatagg caaattcgat tttgcctccg caggagtgtt ttcatggaat ttaccaccgg
     7501 attgatgtcg ctcgacaccg cgcttaatga gatgctttct cgcgttaccc cactgaccac
     7561 ccaggaaacg ctgccactgg ttcagtgttt tggtcgtatt ctggcgagcg atgtcgtttc
     7621 gccactggat gtcccggggt ttgataactc cgctatggac ggctacgcgg tgcgtttagc
     7681 cgatattgcc tccgggcaac cgctgcccgt tgccggtaaa tcctttgccg gtcagccata
     7741 ccatggtgaa tggcctgcgg gtacctgcat tcgtattatg accggtgcgc cggtgccgga
     7801 aggctgcgaa gcggtggtga tgcaggagca gactgaacaa acggacaatg gcgtgcgttt
     7861 tactgctgaa gtgcgtagcg ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg
     7921 tgcggttgtt ttcccggcgg gaactcgcct gactaccgca gagctgccag taattgcttc
     7981 actggggatt gccgaagttc cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg
     8041 tgatgaactc cagttgcccg gtcagccgct gggcgacggc caaatctacg acaccaaccg
     8101 tctcgccgta cacctgatgt tagaacagct gggttgcgag gtaatcaact tagggattat
     8161 ccgcgacgat ccacatgccc tgcgcgccgc atttattgaa gccgacagcc aggcggatgt
     8221 agtgatcagt tccggcggtg tttcagtggg tgaagcggat tacaccaaaa cgattcttga
     8281 agagctgggg gagatcgcct tctggaagct ggcgattaaa ccaggtaaac cgttcgcatt
     8341 cggtaaactc agcaatagct ggttctgcgg cctgccgggc aacccgattt cagcgacgct
     8401 gaccttctat caactggtac agcctttgct ggcaaaacta agcggcaaca ccgccagcgg
     8461 cctgcccgcg cgccagcgcg tacgcacagc gtcccgcctg aaaaaaacgc caggacgtct
     8521 tgatttccag cgcggcgtgc tgcaacgcaa cgccgacggt gaacttgaag tgacgaccac
     8581 cggacatcag ggttcacata tatttagctc ctttagtctc ggcaactgct ttatcgtgct
     8641 ggaacgcgat cgcggcaatg tggacgtggg cgaatgggtg gaagtagaac cgtttaacgc
     8701 gttgttcgga ggcctgtaat ggcggaactc agcgatcagg agatgctgcg ctacaaccgg
     8761 caaatcattc tgcgcggttt tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg
     8821 ctggtagtcg gcctcggtgg actcggctgc gcagcttcgc agtatctggc aagcgccggt
     8881 gtcggcaacc tgacgctgct cgacttcgac acggtttcgc tctcgaatct gcaacgccag
     8941 acactgcaca gtgatgccac ggtcgggcaa ccgaaggtgg aatccgcccg tgacgccctg
     9001 acgcggatca acccacatat cgcgattacg ccagtcaatg cactgctaga tgacgcagaa
     9061 cttgcagcat tgattgctga acacgatctg gtgctcgact gtacggataa cgttgcggta
     9121 cgtaatcaac tgaacgcagg ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca
     9181 attcgtatgg aagggcaaat cacggtcttc acttatcagg acggcgaacc gtgctatcgc
     9241 tgccttagcc gtttgtttgg cgagaacgcg ttaacctgcg tagaagcagg cgtgatggca
     9301 ccgttgatcg gcgtaattgg ttcgttgcag gcaatggaag cgatcaaact gctggcaggt
     9361 tatggcaaac cggccagcgg gaaaatcgtc atgtacgatg cgatgacctg tcagtttcgc
     9421 gaaatgaaac tgatgcgcaa tccggggtgt gaggtgtgcg ggcagtaatt gccacctcca
     9481 ggcctgataa gacgcgacaa gcgtcgcatc aggcatttgc tccagacgcc agatgcggcg
     9541 tgaacgcctt atctggccta cggttcgggc acagatttcc aagcgcggca ggcatcggtg
     9601 cactccgcca cgtcatcagc aatttatgag gatgcagaac gtccggttaa atcgacgttc
     9661 tgccaaacgc tccctgccag tcctgctcaa acttcgccac agcggcttcc accgccggat
     9721 agctaatcat ctgttgtgcc acatccagtg gcagagtaat tgattcacat ccggccagta
     9781 agcagtccag cgcctgacgc ggggttttga aactcgctgc cagcactttc gcctgcggcg
     9841 catgcatttt caataactgg tgtaagtcgg tcacagtctg aatgccgcta ccgccctgag
     9901 catcaatacg attaacgtaa ggcgcaacat attccgcacc tgccagcgcc gacagcagcc
     9961 cttgtgccgc gccatatacc gcggttccca gcgtcggaat cccttccgct tttaacatct
    10021 taatcgctgc cagcccctcg gctgtcaccg gaactttcac cacgatatcc gcaataatag
    10081 aacgcagctt aagcgcgtca ttcaccatcc cttcagcagt ggtagccatt acctgggcaa
    10141 acagacgccc ctgaccgccc atcgcttcat gaagttgcgg aagcacaaca tccagcggtt
    10201 ttttacccgc ggcgataatg cttgggttag tggtcacacc cgccagcgga aaaatacgtg
    10261 acagcgcctt caccgcaaca acgtctgaag tatccagata cagttccata accatcctca
    10321 aaatgtactt tttgtctgaa tgtagactat cacgggaaaa ctctctgaac agttgacgca
    10381 catcaatcta actttcattc gaaagtattt taatgtttga acgaaacaga gaggcaacta
    10441 tgattttcaa tattcagcgc tactcgaccc atgatggccc cggtatccgc acggtcgtat
    10501 ttcttaaagg ctgttcgctg ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgta
    10561 cgcaggatct gctgtatgac gcacgactgt gtctggaagg ctgcgagctg tgcgctaagg
    10621 ccgcgccgga agtgattgag cgcgcgctga atggtttgct tattcatcgg gaaaagttaa
    10681 ccccggagca tctgacggcg ttaaccgact gctgtccgac acaggcgtta accgtgtgtg
    10741 gtgaagtgaa aagcgttgag gagatcatgg cgaccgttct gcgcgataaa ccgttttacg
    10801 atcgcagcgg cggcggctta acgctttcgg gtggtgaacc cttcatgcag ccggaaatgg
    10861 cgacggcgct gctgcaagcc tgccacgagg caggcattca tactgcggta gaaacctgtc
    10921 tgcatgtgcc gtggaaatat atcgcccctt ctctgcccta tatcgatctg tttcttgccg
    10981 atttaaaaca cgttgccgac gcgccgttta aacagtggac cgacggtaac gccgccaggg
    11041 tgctggataa cctgaaaaaa ctcgccgcag cgggcaaaaa aatcattatc cgcgtgccgc
    11101 tgattcaggg ctttaatgcc gacgaaacct ctgtaaaagc cattaccgat tttgccgccg
    11161 acgagctgca cgttggcgaa attcattttc tgccctacca cacgctgggc atcaacaaat
    11221 atcacttact taatctgccc tatgacgccc cggaaaaacc gcttgatgcg ccagagctgc
    11281 tcgattttgc ccagcagtat gcctgccaga aagggttaac cgcgacctta cgaggataac
    11341 aaccatgacc acactgaaac tggacacgct cagcgaccgc attaaagcgc acaaaaatgc
    11401 gctggtgcat attgtgaaac cgccagtctg taccgagcgc gcgcagcact ataccgagat
    11461 gtatcaacaa catctcgata agccgatccc ggtacgtcgc gcgctggcac tggcgcatca
    11521 cctggcgaat cgcaccatct ggatcaaaca ctatgagttg atcattggca accaggcaag
    11581 cgaagttcgc gccgcgccga tcttcccgga atatactgtc tcgtggatcg aaaaagagat
    11641 tgatgatctg gcagatcgtc ccggtgctgg ctttgcggtg agcgaagaga acaaacgtgt
    11701 tctgcatgaa gtgtgcccgt ggtggcgcgg tcagacggta caggatcgct gctatggcat
    11761 gtttaccgat gagcaaaaag gtctgctggc gaccggcatc attaaagcgg aaggcaatat
    11821 gacctccggc gatgcgcacc tggcggtcaa tttcccgctg ctgctggaaa aagggcttga
    11881 tggtctgcgt gagaaagtag cggaacgtcg ctcgcgcatc aatctgacgg tgctggaaga
    11941 tttgcacggc gaacagttcc tgaaagcgat tgatatcgtg ctggtggcag tcagtgaaca
    12001 cattgaacgt ttcgctgccc tggcgcgtgc aatggccgcg accgaaaccc gcgaaagccg
    12061 tcgcgatgaa ctgctggcga tggcagaaaa ctgcgatctt atcgcccacc agccgccgca
    12121 gactttctgg caggcgctgc aactgtgtta cttcatccag ttgattttgc agattgaatc
    12181 taacggtcac tcggtgtcgt ttggtcgtat ggatcagtat ctctacccgt actatcgccg
    12241 cgacgttgaa ctgaaccaga cgctggatcg cgaacacgcc atcgaattgc tgcatagctg
    12301 ctggttgaaa ttgctggaag tgaacaaaat ccgctccggc tcccactcaa aagcctctgc
    12361 gggaagtccg ctgtatcaga acgtcaccat tggcggacaa aatctggttg atggtcaacc
    12421 aatggacgcg gtgaatccac tctcttacgc gatcctcgaa tcctgcggtc gcctacgttc
    12481 cactcagcct aacctcagcg tgcgttacca tgcaggaatg agtaacgatt tcctcgacgc
    12541 ctgcgtacag gtgatccgct gcggcttcgg gatgccggca ttcaacaacg acgaaatcgt
    12601 gatcccggaa tttattaaac tcggtattga accgcaggac gcttatgact acgcagcgat
    12661 tggttgtata gaaaccgccg tcggtggcaa atggggctat cgctgtaccg gcatgagctt
    12721 tatcaacttc gcccgcgtga tgctggcggc gctggaaggc gggcgtgatg ccaccagcgg
    12781 caaagtgttc ctgccacaag aaaaagcgtt gtcggcaggt aacttcaaca acttcgatga
    12841 agtgatggcc gcgtgggata cgcaaatccg ttactacacg cgtaaatcaa tcgaaatcga
    12901 atatgtcgtc gacaccatgc tggaagagaa cgtgcacgat attctctgct cggcgctggt
    12961 ggatgactgt attgagcgag cgaaaagtat caagcaaggc ggcgcgaaat atgactgggt
    13021 ttctggcctg caggtcggta ttgccaacct cggcaacagc ctggcggcag tgaagaaact
    13081 ggtgtttgaa cagggcgcga ttggtcagca acagctggct gccgcactgg cggatgactt
    13141 cgatggcctg actcatgagc aactgcgtca gcggctgatt aacggtgcgc cgaagtacgg
    13201 caacgacgat gataccgtcg atacgctgct ggctcgcgct tatcagacct atatcgacga
    13261 actgaaacag taccataacc cacgctacgg tcgtggtccg gttggcggca actattacgc
    13321 gggtacgtcg tctatctccg ctaacgtacc gtttggcgcg caaactatgg caacgccgga
    13381 cgggcgtaaa gcccacaccc cgctggcaga aggcgctagt ccggcctctg gtactgacca
    13441 tctcggccct actgcggtca ttggctcagt gggtaaactg cctacggcag cgattctcgg
    13501 cggcgtgttg ctcaaccaga aactgaatcc ggcaacgctg gagaacgaat ctgacaagca
    13561 gaaactgatg atcctgctgc gtaccttctt tgaagtgcat aaaggctggc atattcagta
    13621 caacatcgtt tcccgcgaaa cgctgctgga ggcgaaaaaa catccggatc agtaccgcga
    13681 tctggtagtg cgtgtcgcgg gttattccgc cttcttcacc gcgctctctc cagacgctca
    13741 ggacgatatc atcgcccgta ctgaacatat gctgtaatct cttcggcccg gctgcggcgt
    13801 cgccgggcta aatcattcac atcatcaatt tcatccttac tttcattcga aatataattt
    13861 gtgctctgcg tcacattgtt attacactgt tacggtttca ttttcgagcc aggagcacac
    13921 ctatgagcgt aaaagttatc gtcacagata tggacggtac ttttcttaac gacgccaaaa
    13981 cgtacaacca accacgtttt atggcgcaat atcaggaact gaaaaagcgc ggcattaagt
    14041 tcgttgtcgc cagcggtaat cagtattacc agcttatttc gttctttcct gagctaaagg
    14101 atgagatctc ttttgtcgcg gaaaacggcg cactggttta cgaacatggc aagcaactgt
    14161 tccacggcga actgacccga catgaatcgc ggattgttat tggcgagttg ctaaaagata
    14221 agcaactcaa ttttgtcgcc tgcggtctgc aaagtgcata tgtcagcgaa aatgcccccg
    14281 aagcatttgt cgcactgatg gcaaaacact accatcgcct gaaacctgta aaagattatc
    14341 aggagattga cgacgtactg ttcaagtttt cgctcaacct gccggatgaa caaatcccgt
    14401 tagtgatcga caaactacac atagcgctgg atggcattat gaaacccgtc accagtggtt
    14461 ttggctttat cgacctgatt attcccggtc tacataaagc aaacggtatt tcgcggttac
    14521 tgaaacgctg ggatctgtca ccgcaaaatg tggtagcgat tggcgacagc ggtaacgatg
    14581 cggagatgct gaaaatggcg cgttattcct ttgcgatggg caatgctgcg gaaaacatta
    14641 aacaaatcgc ccgttacgct accgatgata ataatcatga aggcgcgctg aatgtgattc
    14701 aggctgtgct ggataacaca tcccctttta acagctgacc tctctaccgg agccatgcgc
    14761 tccggttttc ccttcttttt acatcctgta catcacatca aatcattaaa cttgcattaa
    14821 cttatttata acttgtattg tcacatgcaa atcagcatag tttcgaatga aagttaatgc
    14881 gaggtggtta tggcttctac ttttaccagc gacacattgc ctgccgatca caaagcagct
    14941 atccgtcaga tgaagcacgc gctgcgggcg cagcttggcg atgtccagca gatctttaat
    15001 cagctaagcg atgacattgc cacgcgagtg gctgaaatca acgcactcaa agcacagggc
    15061 gatgccgtct ggccggtgct gtcttatgcc gatatcaaag caggtcatgt tactgcagag
    15121 cagcgcgaac agattaaacg tcgcggttgt gcggtgataa aaggccattt cccccgcgaa
    15181 caagcgctag gctgggatca gtcgatgctg gactatctga accgcaaccg ctttgacgag
    15241 gtctacaaag gccccggcga taatttcttc gggacgctca gcgcttcacg tcccgagatt
    15301 taccccatct actggtcgca ggcgcaaatg caggcccgcc agagtgaaga aatggcgaat
    15361 gcgcagtcgt ttctcaatcg tctgtggaca tttgaaagtg atggaaagca atggtttaac
    15421 ccggatgtga gcgtcatcta ccctgaccgt atccgccgcc ttccgcccgg aacgacctcc
    15481 aaaggtcttg gagcgcatac cgactccggg gcgctggaac gctggctgct tccagcgtat
    15541 cagcacgttt tcgccaacgt ctttaatggc aatctggcga aatacgatcc ctggcatgcg
    15601 gcacatcgta cggaagttga agagtacacg gtggacaaca ccaccaaatg ttccgtgttt
    15661 cggacattcc agggctggac tgcgctctcg gatatgctgc ctggtcaggg gttgctgcac
    15721 gtcgtgccca ttcctgaagc catggcgtac gtactgttac gtccgctgct tgatgatgtg
    15781 ccggaggatg aactgtgcgg cgtagcgccc ggaagagtgt tgccggtatc agagcaatgg
    15841 catccactgt taattgaggc gttaaccagc attccaaaac tcgaggccgg agactccgtc
    15901 tggtggcact gcgacgtcat ccattccgtt gcccccgttg aaaatcaaca gggttggggc
    15961 aacgtgatgt acattcctgc ggcaccgatg tgcgagaaaa atcttgccta cgcgcacaag
    16021 gtgaaggccg cactggaaaa aggcgcatcg ccgggcgact tcccgcgcga ggactatgaa
    16081 acaaactggg aaggacgctt tacgcttgcc gacctcaaca ttcacggtaa gcgagcgttg
    16141 ggcatggatg tttgagagtt aatggaagtg aattgatgtg ggattaagaa aatcgattcg
    16201 acgttactca gattgctgac aagtgcgctt tcttaatgcc tgatgcggcg ttaacgcctt
    16261 atccggccta caaagttctg cagattcaat atattacagg tactgcgtag gcctgataag
    16321 cgtagcgcat caggctattt tgcatttgtc atcagccgtc gtcaccccga cagtatcggg
    16381 gtgacggata atcacttact cgatcccttt actacgcagg taatcttcgt aattaccgct
    16441 aaagtcgatc acgcattccg gggtgatttc cagaatgcgg gtcgccaggg agcttacgaa
    16501 ctcacggtcg tgtgaaacaa agatcagcgt gccctgatac agttccagcg ccatgttcag
    16561 tgactcgatg gattccatat ccaggtggtt ggtcggttcg tccatgatca gaatgttcgg
    16621 cttctgcatc attaacttac caaacagcat ccgccctttt tccccaccgg aaagcacttt
    16681 agctggcttt ttgatgtcgt cctggctgaa cagcaaacga ccgagaatgc tgcgtaccgc
    16741 ctgctcgtca tcgccttcct gcttccactg actcatccat tcgaacacgg tcagatcatt
    16801 ttcaaactca tattcgtggt cctgggcgta gtaaccaatg cgcgcgtttt cagaccattt
    16861 tacggtgccg ctgtccggtt gcagatcgcc caccagcgtt ttcagcagcg ttgatttacc
    16921 gacgccgttg gtacccagta ccgcaagttt ttcaccgacc tccagcagca ggttgagatt
    16981 tttaaacagt ggaccgttat caaacccttt ggtcagacct tccacttcca gcgcgttacg
    17041 gaacagtttc ttatcctgtt caaaacggat gaacgggttc tgacggctgg aggctttcac
    17101 ctcttccagt ttgattttat caatctggcg cgcgcgcgaa gttgcctggc gagatttcga
    17161 ggcgttggcg ctaaagcggc taacgaaaga ttgcaactca gcaatctgcg ctttcttctt
    17221 ggcgttatcg gccagcagac gttcacgcgc ctgggtcgcc gccgtcatgt actcatcgta
    17281 gttacccgga taaacgcgca gctcgccgta atccagatcc gccatgtggg tacagaccat
    17341 gttaaggaag tgacggtcgt gcgagatgat gatcatggtg ctgtcacgct cgttcagcac
    17401 ctgttccagc cagcgaatgg tgtcgatgtc gaggttgttg gtcggttcgt cgagcaggag
    17461 aatatccggc tcagcaaaca gcgcctgcgc cagaagcaca cgcagcttcc agccaggagc
    17521 aacttcactc atcgggccgt agtgctgttc cactggaatt cccacgccaa gcaacagttc
    17581 accggcgcga gcttccgcag agtaaccgtc catttcgccg tatttcactt ccagatcggc
    17641 cactttatag ccgtcttctt cactcatttc cggcaaagca tagatgcggt cgcgctcctg
    17701 cttcacttcc cacaactctt tatgccccat gatcaccgta tccagcacag tgaacccttc
    17761 aaaggcaaac tgatcctgac gcagcttacc aatgcgctcg ttgggatcga gggaaacgtt
    17821 acccagcgtc ggctcaaggt cgccgccgag aatcttcata aaggtggatt taccactacc
    17881 gttcgcgcca atcaggccgt aacggttgcc gccgccgaat ttgacggaaa tgttttcaaa
    17941 caacggctta ctgccgaact gcatggtgac gttactggaa actaacacag gcgtatcctg
    18001 aaaagagata tgacaaaccg cgcattatgc cataagtctg aaagaagatc ctgctcctcg
    18061 cttaaggtct acactcaagc caaaggcaaa aaatgtgatt tcgtacacat ctgatttcac
    18121 tgtgagctgg aatgaactta taatgcgctt ccaatactct aatattctca acccaatggc
    18181 ctgccaggca caaaatctcg cttaacatga atatgaaatt gaaaacatta ttcgcagcgg
    18241 ccttcgctgt tgtcggcttt tgcagtaccg cctctgcggt aacttatcct ctgccaaccg
    18301 acgggagtcg cctggttggt cagaatcagg tgatcaccat tcctgaaggt aacactcagc
    18361 cgctggagta ttttgccgcg gagtaccaga tggggctttc caatatgatg gaagcgaacc
    18421 cgggtgtgga taccttcctg ccgaaaggcg gtactgtact gaacattccg cagcagctga
    18481 tcctgccgga taccgttcat gaaggcatcg tcattaacag tgcagagatg cgtctgtatt
    18541 actatccgaa agggaccaac accgttatcg tgctgccgat cggcattggt cagttaggca
    18601 aagatacgcc tatcaactgg accaccaaag ttgagcataa gaaagcaggc ccgacctgga
    18661 cgccgaccgc caaaatgcac gcagagtacc gcgctgcggg cgaaccgcta ccggctgtcg
    18721 ttccggcagg tccggataac ccgatgggtc tgtatgcact ctacatcggt cgcctgtatg
    18781 ctatccatgg caccaacgcc aacttcggta tcggcctgcg tgtaagtcat ggttgtgtgc
    18841 gtctacgtaa cgaagacatc aaattcctgt tcgagaaagt accggtcggt acccgcgtac
    18901 agtttattga tgagccggta aaagcgacca ccgagccaga cggcagccgt tatattgaag
    18961 tccataaccc gctgtctacc accgaagccc agtttgaagg tcaggaaatc gtgccaatta
    19021 ccctgacgaa gagcgtgcag acagtgaccg gtcagccaga tgttgaccag gttgttcttg
    19081 atgaagcgat caaaaatcgt tccgggatgc cggttcgcct gaattaatcg tcaaaacgta
    19141 aaacaaaggc ggactcataa tccgcctttt ttatttgcca gaccttacca ggccgggatc
    19201 ataactaaca aaacgtatcc caccagcgcc gcccacaaca gcatcggtat tgaatagaga
    19261 tggaaacgcc accagatgcg gcgatcgttc gccatacgta gcgcaatcaa atttgccagc
    19321 gatcctggta ataaaccaaa gccccccacg tttaccgccc atgccagtaa taaagacgga
    19381 ggtacataat tgagcagcaa aatggtactc ggcacgttac tgatcacctg cgataaaccg
    19441 actgccgtta accataaccc gggttcagat aaatgactca cgttacccaa cacgccttgc
    19501 aacgctggaa gctgggtcag taaatggacg tcgataaaca tcgccataaa caccagcagc
    19561 aacgtccagt ccacactcaa caccacgcga cgcgccagca gcacaaagcc cgccgccaca
    19621 atcaccagtc cccacagctc ttgtttgaac tccagcgccg tcagaaaaac gatatacagc
    19681 cccagacaat tccacaccag ccgcggtttc cactccggtg tttgcatccc cgtatggtat
    19741 tgcagcgcct ttccagggaa acaacaccag cacaacagca ggagtgtcag catcattgcg
    19801 ccagccagcg gtgccatttg ggcaataaat ccggcaaacg aaagaccaga acgtccccag
    19861 ataagaatat tttgcgggtt gccaattggc gtcagtagcg aaccagcgtt gactgccagc
    19921 gcctcaaaaa taatcagccg attaaccggg atctcacaca gtctttttag cgtgatagtc
    19981 agcggaacaa caataaacag cgcgacatcg ttggtcagaa aggtagaaag cagcgccgcc
    20041 gccagcacca taaacatcgc cagccgacgc tccgtagcaa agcggcgcac cattttgcgc
    20101 cccagcacat caaaataacc gcttaactcc acacctttgg tcagcagcat caggccgctt
    20161 aaggtgatga tggtgtgcca gtcgatagca gcaggccagg atttcggtgc aaagggcacg
    20221 aaaaagctta atccgatacc aacaagaatt aataactgaa aaaaacgatc gccttgcagc
    20281 gtgcgtaaaa aaggcaggct catttggcac cgtgtttctg ggtaaacaaa cgaaaagcgt
    20341 cgagcgtctc ttcactaaca tggtgctcca tgccttccgc gtcgcgacgg gcgatttccg
    20401 gactgacgcc caacaccaac aagaaatttt cgactatctg atgtcgctcg cggctttcct
    20461 gcgccagctt ctctccttct gccgttaaaa acacgccacg ccaggggatc atttcaatca
    20521 gccccatggt tgccagccgc ttaagcattt tagccaccgt cggttgcgaa actcccagac
    20581 gagcagccat gtccacctga cgagcttccc ccacttccct gatcaagtca gaaatcagct
    20641 caacgtaatc atcaataagc tcgcgccgat gcgcctctct gacctggcgg aacccttcaa
    20701 cgtgctcttc cacgttcact aactgcgtca ctttttttgc tgttggcgta cctgcgcgac
    20761 gactcattgt gcttcctcat tatggtgacg catacatagc gttctcattt cgtgcgcaca
    20821 ttgtaaacca gagttgcgaa ggtacaaaaa attaacgttt tagcaatagc tatataatat
    20881 agcctgtgct atatctgtat gtaatgcaat catccctcaa ggatcgacgg gattagcaag
    20941 tcaggaggtc ttatgaatga gttcaagagg tgtatgcgcg tgtttagtca ttctcccttt
    21001 aaagtacggt taatgctgct ctctatgttg tgcgatatgg tcaacaacaa accgcagcaa
    21061 gataaacctt cagataaata gcggcgtcgc ggtacgccgc ttcactcctg cttttatgca
    21121 ggcataacgc gttttggtct gaaaaaaacc actttttgtc ggatttgcaa tccccttcgc
    21181 aaaagatttg ttcgtcagta gttgacctga acggcggctc gctctatctt cttgcagccc
    21241 tgcgtatatt gcggctcgcg gatgcggacc cctttcccct cttcacgcac tcttgcaggt
    21301 attgaccctt gacgccaggg taagcacatg gcgtttgtta ccatagtggc atatgaattt
    21361 aaccctcaaa gaatcgcttg ttacccgtag ccgggtattt agcccgtgga ctgcgttcta
    21421 ctttttacag tcgctattaa ttaacctcgg cttaggttac cccttcagtt tgctctacac
    21481 cgctgcgttt acggctattt tgcttttgct atggcgaaca ttgcctcgcg tacaaaaagt
    21541 tctggtcggt gtcagttcgc tggtggcggc ttgttatttc ccttttgctc aggcctacgg
    21601 cgcgcctaat ttcaatacat tgctggcatt gcactccacc aatatggaag agtcgaccga
    21661 aatcctgacg atttttccgt ggtacagcta cctggtcggc ttatttattt ttgcgctcgg
    21721 cgtaatagca atcaggcgaa aaaaagagaa tgaaaaagcg cgctggaata ccttcgacag
    21781 cctgtgtctg gtattcagtg tggcgacatt ttttgttgct cccgtgcaaa acctggcctg
    21841 gggtggcgta tttaaactga aagatactgg ctatccggta tttcgttttg ctaaggatgt
    21901 catcgtcaat aataacgagg tgattgaaga gcaagaacgg atggcaaaac tttccggaat
    21961 gaaagatacc tggacggtca ctgccgttaa gccgaagtat cagacctatg tggtagtgat
    22021 cggtgaaagc gcgcgtcgcg atgccctcgg tgcctttggc ggtcactggg acaatacccc
    22081 gtttgccagc agcgttaacg gtttgatatt tgctgactac attgccgcca gtggctccac
    22141 gcagaaatcg cttggcttaa cgctcaatcg cgtagtcgat ggcaaaccac agtttcagga
    22201 taactttgtc accctggcaa atcgcgcggg cttccagacc tggtggtttt ccaaccaggg
    22261 ccaaatcggc gaatacgata ctgctatcgc cagcatcgcc aaacgtgcag atgaagtgta
    22321 cttcctgaaa gaaggtaatt ttgaagcaga taaaaacacg aaagacgaag cgttactgga
    22381 tatgaccgct caagtgctgg cgcaagagca ctcgcaaccg cagctgattg ttctgcatct
    22441 gatgggctcg catccgcagg cctgcgacag gacacaaggc aaatacgaaa cctttgtgca
    22501 atcgaaagaa acgtcgtgct atctctatac catgacgcaa acggacgatt tactgcgcaa
    22561 gctgtacgat cagttacgca acagcggcag cagcttctct ctggtttact tttctgacca
    22621 cggtctggcc tttaaagagc gcggcaaaga cgtgcaatac cttgcccatg atgataaata
    22681 tcagcaaaat ttccaggtgc cttttatggt catttccagc ggcgataaag cgcatcgggt
    22741 gattaaagcc cgccgctcag ccaatgactt cttaggcttt ttctcgcagt ggacgggaat
    22801 taaagcgaag gaaataaata tcaaataccc gtttatatct gagaagaaag ccgggccgat
    22861 atacatcacc aacttccagt tacagaaggt ggattacaac catctcggaa ccgatatttt
    22921 cgacccgaaa ccttaaaaac aaaaatccgc cccgagaggc ggatttttat atcaccaaag
    22981 tgattagaag cggtaaccaa caccggcaat ccaggtgcct acgtcaacgc tacgaatacg
    23041 gctctgctcg taagagaagt ccagagcaac gttttccatc gggttgaact gcagaccagc
    23101 accgtaggag aaaccgtagt cgctggtgtc gtgtttgtag gtcgggtatt cagtggtctg
    23161 gaatttacca taacccacac ccactacacc gtagatgctt gcccagtcgt taatgcggta
    23221 agccggacca gcagtgatgc cgtagtactg gtttttgttg tagtcaccag agcttgcagt
    23281 acggcttttc tcggtgtaag tgaaagaacc gatcacaccc agcgggctgt tgtcttcttc
    23341 atagcggtat ttcaggttga aaccgcccat tttgttcatt tggccctgag cgtcgctctg
    23401 tgcgtaaccg ccagttacgg tagaagtcgc agctacggaa gtgcctgcgg tgaaagccag
    23461 aactgcggcc agtgctgaaa gacatgcaat ttttttcata accacctcaa atgtgattca
    23521 aataagtcct aagttttaaa tatatcaaaa attaatggga aactcttcgc gatttgtgat
    23581 gtctaacggg ccatttcatg taacagaacg tttccataca ccgctatcca tctaaattta
    23641 aatcactttt tcagagaact gcgtaagtat tacgcatgtt ttccctgtca ttcatccaga
    23701 ttattcctaa tcaccagact aatgattcca tcaatcctgg cgcattttag tcaaaacggg
    23761 ggaaaatttt ttcaacaaat gctcaaccag cattgggtat atccagtaca ctccacgctt
    23821 tacttaagtc tagatatttg tgggagaaag gatgcctggt tcattacgta aaatgccggt
    23881 ctggttacca atagtcatat tactcgttgc catggcgtct attcagggtg gagcctcgtt
    23941 agctaagtca ctttttcctc tggtgggcgc accgggtgtc actgcgctgc gtctggcatt
    24001 aggaacgctg atcctcatcg cgttctttaa gccatggcga ctgcgctttg ccaaagagca
    24061 acggttaccg ctgttgtttt acggcgtttc gctgggtggg atgaattatc ttttttatct
    24121 ttctattcag acagtaccgc tgggtattgc ggtggcgctg gagttcaccg gaccactggc
    24181 ggtggcgctg ttctcttctc gtcgcccggt agatttcgtc tgggttgtgc tggcggttct
    24241 tggtctgtgg ttcctgctac cgctggggca agacgtttcc catgtcgatt taaccggctg
    24301 tgcgctggca ctgggggccg gggcttgttg ggctatttac attttaagtg ggcaacgcgc
    24361 aggagcggaa catggccctg cgacggtggc aattggttcg ttgattgcag cgttaatttt
    24421 cgtgccaatt ggagcgcttc aggctggtga agcactctgg cactggtcgg ttattccatt
    24481 gggtctggct gtcgctattc tctcgaccgc tctgccttat tcgctggaaa tgattgccct
    24541 cacccgtttg ccaacacgga catttggtac gctgatgagc atggaaccgg cgctggctgc
    24601 cgtttccggg atgattttcc tcggagaaac actgacaccc atacagctgc tggcgctcgg
    24661 cgctatcatc gccgcttcaa tggggtctac gctgacagta cgcaaagaga gcaaaataaa
    24721 agaattagac attaattaaa tttacatttc tgtatggtta tgcataacca tgcagaattt
    24781 ctcgctactt ttcctctaca ccgtctttat atatcgaatt atgcaaaagc atatttattc
    24841 cgaaaattcc tggcgagcag ataaataaga attgttctta tcaatatatc taactcattg
    24901 aatctttatt agttttgttt ttcacgcttg ttaccactat tagtgtgata ggaacagcca
    24961 gaatagcgga atacatagcc ggtgctatac ttagtctcgt taattactgg gacataacat
    25021 caagaggata tgaaattatg agtaccgcta aattagttaa atcaaaagcg accaatctgc
    25081 tttatacccg caacgatgtc tccgacagcg agaaaaaagc aacagtagag ttgctgaatc
    25141 gccaggttat ccagtttatt gatctttctt tgattaccaa acaagcgcac tggaacatgc
    25201 gcggcgctaa cttcattgcc gtacatgaaa tgctggatgg cttccgcacc gcactgatcg
    25261 atcatctgga taccatggca gaacgtgccg tgcagctggg cggtgtagct ctggggacca
    25321 ctcaagttat caacagcaaa accccgctga aaagttaccc gctggacatc cacaacgttc
    25381 aggatcacct gaaagaactg gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag
    25441 cgattggcga agcgaaagat gacgacaccg cagatatcct gaccgccgcg tctcgcgacc
    25501 tggataaatt cctgtggttt atcgagtcta acatcgaata aatccatcgc tgatggtgca
    25561 gaactttagt acccgataaa agcggcttcc tgacaggagg ccgttttgtt ttgcagccca
    25621 cctcaacgca cttatttagt gcatccatct gctatcccca gctgattaag taaatttttt
    25681 gtatccacat catcacacaa ccgttacata aagattgttt tttcatcagg ttttacgcta
    25741 aataatcact gtgttgagtg cacaatttta gcgcaccaga ttggtgcccc agaatggtgc
    25801 atcttcaggg tattgctcta taaatcgtgc atcacgtttt tgccgcatct cgaaaaatca
    25861 atgagttgca aaactggcac gattttttca tatatgtgaa tgtcacgcag gggatcgtcc
    25921 cgtggataga aaaaaggaaa tgctatgaag tctgtattaa aagtttcact ggctgcactg
    25981 accctggctt ttgcggtttc ttctcatgcc gcggataaaa aattagttgt cgcgacggat
    26041 accgccttcg ttccgtttga atttaaacag ggcgataaat atgtgggctt tgacgttgat
    26101 ctgtgggctg ccatcgctaa agagctgaag ctggattacg aactgaagcc gatggatttc
    26161 agtgggatca ttccggcact gcaaaccaaa aacgtcgatc tggcgctggc gggcattacc
    26221 atcaccgacg agcgtaaaaa agcgatcgat ttctctgacg gctactacaa aagcggcctg
    26281 ttagtgatgg tgaaagctaa caataacgat gtgaaaagcg tgaaagatct cgacgggaaa
    26341 gtggttgctg tgaagagcgg tactggctcc gttgattacg cgaaagcaaa catcaaaact
    26401 aaagatctgc gtcagttccc gaacatcgat aacgcctata tggaactggg caccaaccgc
    26461 gcagacgccg ttctgcacga tacgccaaac attctgtact tcatcaaaac cgccggtaac
    26521 ggtcagttca aagcggtagg tgactctctg gaagcgcagc aatacggtat tgcgttcccg
    26581 aaaggtagcg acgagctgcg tgacaaagtc aacggcgcgt tgaaaaccct gcgcgagaac
    26641 ggaacttaca acgaagtcta caaaaaatgg ttcagtactg aaccgaaata ataacgctac
    26701 acctgtaaag cgcactggca gttccctctc ccccctgggg agaggattag ggtgaggggc
    26761 gcaaacccgc tccggggcca ttaattaccc tgaatttgat tatttacacc acggtaacag
    26821 gaacaacata tgcagtttga ctggagtgcc atctggcctg ccattccgct tctgattgaa
    26881 ggtgccaaaa tgaccctgtg gatttcggtc ctcggtctgg ctggcggtct ggtaatcgga
    26941 ttgctggcag gttttgcacg caccttcggt ggttggatag ccaaccacgt cgcgctggtc
    27001 tttattgaag tgatccgcgg cacacctatc gtcgtccagg tgatgtttat ctatttcgcc
    27061 ctgccgatgg cgtttaacga cttacgcatc gacccattta ctgcggcggt ggtcaccatc
    27121 atgatcaact ccggcgcgta cattgcggaa atcacgcgtg gtgcggtgct gtctatccac
    27181 aaaggttttc gtgaagcagg actggcgctc ggtctttcac gttgggaaac cattcgctac
    27241 gtcattttac cgctggcact gcgtcgtatg ctgccgccgc tgggtaacca gtggatcatc
    27301 agcattaaag acacctcact gtttattgtt atcggcgtgg cggaactgac ccgtcagggg
    27361 caagaaatta ttgccggtaa cttccgcgcc cttgagatct ggagcgccgt ggcggtgttc
    27421 tatctgatta ttaccctggt gctgagcttt attctgcgtc gtctggaaag aaggatgaaa
    27481 atcctgtgat tgaatttaaa aacgtctcca agcactttgg cccaacccag gtgctgcaca
    27541 atatcgattt gaacattgcc cagggcgaag tcgtggtgat tatcgggccg tccggttccg
    27601 gtaaatcgac cctgctgcgc tgcatcaaca aactggaaga aatcacctcc ggcgatctga
    27661 ttgtcgatgg cctgaaggtt aacgatccga aagttgacga gcgcctgatt cgccaggaag
    27721 caggtatggt gttccagcag ttttacctct tcccgcatct gacggcgctg ggaaacgtca
    27781 tgtttggtcc gctacgcgtg cgtggcgcga acaaagaaga ggcggaaaaa ctggcgcgtg
    27841 agctgctggc gaaagtcggt ctggcagaac gtgcacatca ctacccttcc gaactttctg
    27901 gtggtcaaca gcagcgtgtg gcgattgccc gcgcgctggc ggtgaagccg aaaatgatgc
    27961 tgtttgatga accgacttcc gctcttgacc cggaactgcg ccatgaagtg ctgaaggtta
    28021 tgcaggatct ggctgaagaa gggatgacga tggtgatcgt gacccacgaa atcggttttg
    28081 ccgagaaagt agcttcgcgg ctgatcttta tcgacaaagg ccggattgcg gaagatggca
    28141 atccgcaggt gttgatcaag aacccgccga gccagcgctt gcaggaattt ttgcagcacg
    28201 tctcttaata agacacattg cctgatgcgc tacgcttatc aggcctacag gatatctggc
    28261 aacttattaa aattgcatga acttgtagga cggataaggc gttcacgccg catccggcaa
    28321 aaaagcccgc acgttgtcag caacctgctt aatatccctt cctccctttc acccgaaagg
    28381 gaggcacacc agattcctct catttaaaat cgcccctccc ccagcatcta tacttatctt
    28441 tttgctctgt tttctcactg gaggagtcat gcggtggatc ctgttcatcc tcttctgcct
    28501 gctgggcgca cctgcccacg cggtatccat acccggcgtt acaaccacaa cgacaacgga
    28561 ctcaacgact gaaccggccc cggaaccgga tatcgaacaa aaaaagcggc ctatggcgca
    28621 ctggcggatg tgctggataa cgacacctcg cgtaaagagt tgatcgacca gttgcgtact
    28681 gtcgccgcga cgccccctgc ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa
    28741 gagcaaaccg tgctgcaaaa ggtcaccgaa gtcagccgcc attatggtga agccctttcc
    28801 gcccgcttcg ggcaacttta tcgcaatatc accggctccc cgcataagcc atttaatcca
    28861 caaaccttca gcaatgcgct gagccatttt tcaatgttag cggtattagt gtttggtttt
    28921 tactggctga ttcgcctgtg cgcactgccg ctatatcgca aaatgggtca gtgggcgagg
    28981 caaaaaaatc gtgagcgcag caactggttg cagcttccgg cgatgattat tggggcgttt
    29041 attatcgacc tgctgttact ggccctgaca ttatttgtcg gccaggtatt aagcgacaac
    29101 ctgaatgcag gtagtcgcac catcgctttc caacaaagtt tgtttctcaa cgcctttgcc
    29161 ctgattgaat ttttcaaagc cgtactacgc ctgatttttt gcccaaacgt ggcggagctg
    29221 cgcccgttca cgattcagga cgagagcgcc cgttactgga gtcgccgcct gagctggtta
    29281 agcagcctga ttggctatgg cctgattgtg gccgtgccga ttatctctaa tcaggtgaat
    29341 gtacagatag gtgcgctggc gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg
    29401 tacctgatct tccgaaataa aaaagagatt acccagcatt tgctcaactt tgcggagcat
    29461 tcgctggctt ttttcagcct gtttatccgc gcctttgcgc tggtgtggca ctggctggca
    29521 agcgcctatt ttatcgtgct gtttttcttt tcgttgttcg atccgggcaa cagtctgaaa
    29581 tttatgatgg gtgcaacggt gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc
    29641 ggtatgtttt cccgctggct ggcgaaaacc atcaccctct cgccacatac tcagcgtaac
    29701 tatccggagc tacaaaaacg gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt
    29761 attctgaccg tctgcgtggc agtaatgttg ctgttgagcg catggggatt gttcgatttc
    29821 tggaactggc tgcaaaacgg cgcggggcag aaaaccgtag atatcctgat ccgcatcgca
    29881 ctcattcttt tcttctcggc ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg
    29941 ctggcttcgg atattcatgg ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg
    30001 ctgttccgta acgcgctggc ggtgattatc agtaccatca ccatcatgat tgtgttgtcg
    30061 gaaatcggcg tcaatatcgc gccattgctg gcaggtgccg gggcattagg tctggctatc
    30121 tcgtttggtt cgcaaacgct ggtaaaagat attatcaccg gggtatttat tcagtttgaa
    30181 aacggcatga acactggaga tttggtgact atcgggccgt tgaccggcac agtggaacgg
    30241 atgtcgattc gctccgtggg cgtgcgccag gataccgggg cgtatcacat cattccgtgg
    30301 tcttcgataa ccacctttgc taacttcgtc cgcggtattg gttcggtagt ggcaaactac
    30361 gatgttgatc gccatgaaga tgctgataaa gccaatcagg cactgaaaga tgcggtaacg
    30421 gaattgatgg aaaacgaaga aattcgcggg ctgattattg gtgaaccgaa ctttgccggg
    30481 attgtcggct taagcaatac cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc
    30541 aaacagtgga cggtacgctt tgccctcgac agccaggtga aaaaacattt cgacctggcg
    30601 ggcgttcgcg cgccagtgca gacttatcag gtgctgcctg ctccgggcgc gaccccggct
    30661 gaaccgctgc cgccggggga gccaacgctt taacgttggc gattaacaaa acggcggcgc
    30721 tgctcgtcgt tcataaaggt ccaggcaata aagcgactct gcttttgccc ctgggccatc
    30781 tcttttttta ccaccttcac cgcgcccacg tcggtcagag cacgatacaa cggcggtaag
    30841 ttttcaccac gagataccag cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc
    30901 tcttcaatca tctttttgat aaagttgact tcaccgcctt cacaccacaa ctcctgttgc
    30961 tgaccaccaa agttcagtgc atcgtctttg ttcagcccca ggttacggcg tttacgctca
    31021 ctacctgccc gcgccgcagc ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg
    31081 tattgctcgt ttttgtggat gatgccgtta aaaatcgccc cactctcttt ttgccgacgc
    31141 agacgaatgg cgcggttaag ccccggatta gcactgataa tcgcctgcgc actgcttaac
    31201 gcctggctgc tggtttcact accggtaaaa cgccagccat attcatgtac gccaatcagc
    31261 ggataaatac agttcgcacc aacgccgata tccagaatgc tggcattcgc tggaattgtt
    31321 ccgcttgctt cagccagtaa atcggcaagg tgatgaatat aatccgcccg gcccggtact
    31381 ggtgggcaga gaaaaccgtc ggggatatcc cagttcgcta cggcgtaaaa atgggccagc
    31441 aacgccttat tgagcgcctt caccgccagc ggattggcaa agtctacgct ttgctcccct
    31501 gcgggtgtaa gcgtgaggaa ttgcctgagt tcaggattga cctgacaaag cgtggcgaga
    31561 tcgtagcggc tgtgatgacg gttgcgcgga tgcaaccccg gtttctgggc ggacatagca
    31621 ttctccttta agcagcggcg taagataccc gcataacgcg acagggtaaa tatctggctg
    31681 cgtttatcat tccccaaaac cttaacatgg ctatggttta tggcaattca tcataaaaat
    31741 cagcattatg catgtcattg atttgtatta ttttgtaaaa atatatcgtt caaaaaataa
    31801 gcatatctct gtcaattttt tgcgtcattt gcatcgcgca ggcgacctac acttatcagg
    31861 cactacccgt tgtatttatg aggatgtcat tatgaaaaag tgcctcacac tactgattgc
    31921 caccgtcctg agcggaatct ctctcacggc ttatgccgcg caaccgatga gtaacctgga
    31981 cagcggtcag ctacggcccg ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga
    32041 tctggaggat aaactggcag aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa
    32101 ttccgcaggc ggaaatgacc agatgttcgg tactgcaacc atctacaaat aaccccacca
    32161 acgtaactgc attcgcctct ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa
    32221 atatgaacgc aatgtaaata aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc
    32281 gtcaagtgat aatgcttatc aaaattatta tcacttgcac aaacactatc acgggattaa
    32341 cagtggcatc gcatccgcag agatgctttc tcgtggcagt gaaaatttca acatataaga
    32401 aaaagtcacc tgcaaaatgg aaaacaatcg caatttccct gccagacaat ttcattcgct
    32461 cacgttcttt gccggtcttt gtattggtat tacgcctgtg gcacaggcac tcgccgccga
    32521 agggcaagct aacgcggatg acacgctggt tgtcgaagca tcaacgcctt cgctttatgc
    32581 gccacaacaa tctgccgatc cgaaattctc gcgtccggta gcggatacta cccgcacgat
    32641 gacggtgatt tctgaacaag tgattaaaga tcagggcgca accaacctta ccgacgcgct
    32701 caaaaac
//
LOCUS       DAJVQH010000033        32301 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJVQH010000033 DAJVQH010000000
VERSION     DAJVQH010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.033"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..690
                     /locus_tag="OQM87_001624"
     CDS             <1..690
                     /locus_tag="OQM87_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCS1507797.1"
                     /translation="QHYYIYDTKFLTLYLEQTEMKNLPIGGVWKGKVKLHSNSPAQDY
                     FANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGF
                     NGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQID
                     VRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLS
                     VIFTPSLPTIN"
     gene            complement(778..2076)
                     /gene="exuT"
                     /locus_tag="OQM87_001625"
     CDS             complement(778..2076)
                     /gene="exuT"
                     /locus_tag="OQM87_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCS1507798.1"
                     /translation="MSKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            2559..3971
                     /gene="uxaC"
                     /locus_tag="OQM87_001626"
     CDS             2559..3971
                     /gene="uxaC"
                     /locus_tag="OQM87_001626"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCS1507799.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHFELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            3986..5473
                     /gene="uxaA"
                     /locus_tag="OQM87_001627"
     CDS             3986..5473
                     /gene="uxaA"
                     /locus_tag="OQM87_001627"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCS1507800.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            5556..6107
                     /locus_tag="OQM87_001628"
     CDS             5556..6107
                     /locus_tag="OQM87_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCS1507801.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(6112..7356)
                     /gene="sstT"
                     /locus_tag="OQM87_001629"
     CDS             complement(6112..7356)
                     /gene="sstT"
                     /locus_tag="OQM87_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCS1507802.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(7952..8917)
                     /gene="alx"
                     /locus_tag="OQM87_001630"
     CDS             complement(7952..8917)
                     /gene="alx"
                     /locus_tag="OQM87_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCS1507803.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(9188..9388)
                     /locus_tag="OQM87_001631"
     CDS             complement(9188..9388)
                     /locus_tag="OQM87_001631"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1507804.1"
                     /translation="MQDLTQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLL
                     TEIRRQTGVIFPADSVKLYLPK"
     gene            complement(9419..10186)
                     /locus_tag="OQM87_001632"
     CDS             complement(9419..10186)
                     /locus_tag="OQM87_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1507805.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSEC"
     gene            complement(10265..10957)
                     /locus_tag="OQM87_001633"
     CDS             complement(10265..10957)
                     /locus_tag="OQM87_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCS1507806.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(11034..11537)
                     /locus_tag="OQM87_001634"
     CDS             complement(11034..11537)
                     /locus_tag="OQM87_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCS1507807.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            11622..12758
                     /gene="rlmG"
                     /locus_tag="OQM87_001635"
     CDS             11622..12758
                     /gene="rlmG"
                     /locus_tag="OQM87_001635"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCS1507808.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            13043..13357
                     /gene="higB"
                     /locus_tag="OQM87_001636"
     CDS             13043..13357
                     /gene="higB"
                     /locus_tag="OQM87_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCS1507809.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            13354..13770
                     /gene="higA"
                     /locus_tag="OQM87_001637"
     CDS             13354..13770
                     /gene="higA"
                     /locus_tag="OQM87_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCS1507810.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(13815..15833)
                     /gene="fadH"
                     /locus_tag="OQM87_001638"
     CDS             complement(13815..15833)
                     /gene="fadH"
                     /locus_tag="OQM87_001638"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCS1507811.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(16053..18404)
                     /gene="ygjK"
                     /locus_tag="OQM87_001639"
                     /pseudo
     CDS             complement(16053..18404)
                     /gene="ygjK"
                     /locus_tag="OQM87_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(18421..18840)
                     /locus_tag="OQM87_001640"
     CDS             complement(18421..18840)
                     /locus_tag="OQM87_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507812.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(18818..19171)
                     /locus_tag="OQM87_001641"
     CDS             complement(18818..19171)
                     /locus_tag="OQM87_001641"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507813.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(19172..19492)
                     /locus_tag="OQM87_001642"
     CDS             complement(19172..19492)
                     /locus_tag="OQM87_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507814.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(19626..21059)
                     /locus_tag="OQM87_001643"
     CDS             complement(19626..21059)
                     /locus_tag="OQM87_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1507815.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(21122..21571)
                     /locus_tag="OQM87_001644"
     CDS             complement(21122..21571)
                     /locus_tag="OQM87_001644"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCS1507816.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATHALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(21568..24654)
                     /gene="ebgA"
                     /locus_tag="OQM87_001645"
     CDS             complement(21568..24654)
                     /gene="ebgA"
                     /locus_tag="OQM87_001645"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCS1507817.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(24837..25820)
                     /gene="ebgR"
                     /locus_tag="OQM87_001646"
     CDS             complement(24837..25820)
                     /gene="ebgR"
                     /locus_tag="OQM87_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCS1507818.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            26039..26370
                     /locus_tag="OQM87_001647"
                     /pseudo
     CDS             26039..26370
                     /locus_tag="OQM87_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(26412..27791)
                     /gene="ygjG"
                     /locus_tag="OQM87_001648"
     CDS             complement(26412..27791)
                     /gene="ygjG"
                     /locus_tag="OQM87_001648"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCS1507819.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            28208..29728
                     /gene="aer"
                     /locus_tag="OQM87_001649"
     CDS             28208..29728
                     /gene="aer"
                     /locus_tag="OQM87_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCS1507820.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(29882..30505)
                     /gene="nfeR"
                     /locus_tag="OQM87_001650"
                     /gene_synonym="yqjI"
     CDS             complement(29882..30505)
                     /gene="nfeR"
                     /locus_tag="OQM87_001650"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCS1507821.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            30782..31528
                     /gene="nfeF"
                     /locus_tag="OQM87_001651"
                     /pseudo
     CDS             30782..31528
                     /gene="nfeF"
                     /locus_tag="OQM87_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(31582..31657)
                     /locus_tag="OQM87_001652"
     tRNA            complement(31582..31657)
                     /locus_tag="OQM87_001652"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31622..31624),aa:Ile,seq:cat)
     gene            31782..32288
                     /gene="mug"
                     /locus_tag="OQM87_001653"
     CDS             31782..32288
                     /gene="mug"
                     /locus_tag="OQM87_001653"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCS1507822.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT         7815 a         8428 c         8497 g         7561 t
ORIGIN      
        1 caacattatt atatatacga tacaaagttt cttacgctct accttgagca gacagagatg
       61 aagaatttgc cgattggtgg cgtctggaag gggaaagtta aattacattc gaacagcccg
      121 gcccaggact atttcgcaaa tattaccctg aatacgctcg accccaacca tattgacgtg
      181 ttcttcccgg agttcgccca cgccacgcca agggtgcagt tagacttgca tccaacagga
      241 agcgttaacg gcagcaacta cgcgcaagat ctgaccatgt tggacatgtg cctgtacgat
      301 ggttttaacg gtaatgccat cagttatgaa atcatgctca aagatgaagg gcgaccagct
      361 gcagggcgca gagacggtta cttctctatc tatcgtcagg gagggaccac caccgacgag
      421 ggagaacgca ttgattaccg ggtcaaaatg tacaacccgg aaaccggtgg gcaaattgat
      481 gtgcgcaata atgaaaatat ggtctggaac agcattaacc tgaaacgtgt gcgtccggtg
      541 gtactgcccg gtattcgcta tgccgtgatg tgtgtgccga cacccttgac tctggcagtc
      601 gataaattca gcgtgatgga taaacaggcc gggtactaca tgggcaaatt gtcagtaatc
      661 tttacgcctt ccttgccaac catcaattaa ccctgcaaac cggacaaacg cttttttcta
      721 tataaacgac gaagccgcct gataaggcgg cttcgtcgtt ataacttacg ttaaacgtta
      781 atgttgcggt gcgggatcgt tatgtgtttc ctgtgccacc tcaatcgccg gtttgttctg
      841 caatacggtc cagataacca gcgcacccag caggtcaaat actgccagaa ctgcgaacag
      901 cgggctgaag ccgatggtgt cagccagtgc accgacaacc agcgcaaaca gcgtacttgc
      961 cagccatgcg gacatcccgg ttaaaccgtt cgccgtcgcc acttcgttac gaccgaacac
     1021 gtcagaagag agcgtaatca gcgcgccaga cagtgcctgg tgggcaaaac caccgataca
     1081 cagcagcata attgcgacat acgggttggt gaacagaccg atcatacccg ggccaatcat
     1141 cagcacagca cccagcgtta cgaccatctt acgggaaacg atcaggttca caccaaacca
     1201 acgctggaac agcggcggca ggtaaccacc gaggatacaa ccgaggtcag caaacagcat
     1261 cggcatccag gcgaacatcg cgatctcttt caggttaaag ccgtaaactt taaacatgaa
     1321 cagcgggatc cacgcgttaa aagtacccca ggccggttct gctaggaaac gcggcagtgc
     1381 gataccccag aactgacggt tacgcaggat ctgaccaacg gacattttct tcgccgtgct
     1441 cacctggtgc tgggcttcct gaccattaat aatatagtcg cgttcttcat cggtcagatg
     1501 cttctggtcg cgcggatgtt tatagaaaat cagccatgcc atcgcccaga taaagctcaa
     1561 tgcaccggag atgataaatg ccatctgcca gctgtgcatc acgattgccc ataccaccag
     1621 cggcggcgca atcatcgcac caatcgaaga acctacgtta aagtagccta ctgcgatgga
     1681 acgctctttc gccgggaacc attcggagct ggctttcaga cccgccggga tcatcgctgc
     1741 ttccgcggca ccgaccgcac cacgagcaac agccaggcca ccccagctac ctgccagcgc
     1801 ggttgcacca cagaacacgg cccacagcac agcaaacatt gcataaccga ttttcgtacc
     1861 cagcacatcc agtacataac ctgctaccgg ttgcatgacc gtataagcag cagaataggc
     1921 tgcgatgata taggaatact gttgggtgga gatgtttaac tcttccatca gagttggcgc
     1981 agctgccgcc acagtgttac gcgtcaggta accaagcacg gtgccgagcg tcaccagtgc
     2041 gatcatatac caacgtaacc ctttaatttt actcatgtaa aacctcatcg ttttgttata
     2101 tccgcaggaa gcgcggccat catcccgcca gaggctgaag atgttagtta cgaccgggta
     2161 accgaaaaaa aaccggcacg ccccgaacgt tgccatgaaa gtttcgtgct aattcggctt
     2221 ccgtaccggt taacccgatt catctaacaa atgaatgaat cggcaatttg cattccgtcg
     2281 acttatgatt tgcgacggca gaaagataac ttgtcataca accttaaaag gtgagagcca
     2341 tcacaaatgt gcgaatattt gtagggacat tacctgacga cagcgaggcc agtactggcg
     2401 cggcctgcag cgagatttac cactttgaga gtaatttttt taactacgtt tattgatcta
     2461 actcacgaaa atatcttcgg actctggaaa ttggtgtgat aactttgtca gcatcgcacc
     2521 ataagcaagc tagctcactc gttgagagga agacgaaaat gactccgttt atgactgaag
     2581 atttcctgtt agataccgaa tttgcccgcc gtctgtatca cgactacgca aaagaccagc
     2641 cgattttcga ttaccattgc catttgccgc cgcagcagat tgcggaagac tatcgtttta
     2701 aaaacctgta tgacatctgg ttgaagggcg atcactacaa atggcgcgct atgcgtacca
     2761 acggtgtggc tgagcgtctg tgtactggtg atgcgtctga ccgtgaaaaa tttgacgcct
     2821 gggcggcgac tgttccgcac actatcggca acccgttata ccactggacg cacttcgaac
     2881 tgcgtcgtcc gttcggtatc actggcaaat tgctttctcc gtcaactgcc gatgaaatct
     2941 ggaacgaatg taacgaactg ctggcgcagg ataacttctc cgcgcgcggc atcatgcagc
     3001 agatgaacgt gaaaatggtc ggcaccaccg atgacccgat cgattctctg gagcatcacg
     3061 cagagatcgc caaagacggc tctttcacca tcaaagtgct gccgagctgg cgtccggaca
     3121 aagccttcaa tatcgaacag gcgaccttta acgactacat ggcgaagctg ggcgaagttt
     3181 ccgataccga cattcgccgc tttgctgacc tgcaaactgc cttgaccaaa cgtctggatc
     3241 acttcgccgc tcacggctgt aaagtgtctg accatgcgct ggacgtggtg atgtttgctg
     3301 aagcgaacga agcggaactc gacagcattc tggcgcgccg tctggctggc gaaaccctga
     3361 gcgagcacga agtggcacag ttcaaaactg cggtactggt gttccttggt gccgaatatg
     3421 cacgtcgcgg ctgggtacag cagtaccaca ttggcgcgct gcgtaataac aacctgcgtc
     3481 agttcaaact gctggggccg gatgtaggct ttgactccat caacgaccgt ccgatggcgg
     3541 aagagttgtc taagctgctg agcaagcaga acgaagaaaa cctgctgccg aaaaccattc
     3601 tctactgcct gaacccgcgc gataacgaag tgctgggcac catgatcggt aacttccagg
     3661 gcgaaggtat gccgggcaaa atgcagttcg gttccggctg gtggtttaac gatcagaaag
     3721 acggtatgga acgtcagatg acccaactgg cgcagctcgg tctgctgagc cgttttgtcg
     3781 gtatgctgac tgacagccgt agcttcctgt catacacccg tcacgaatac ttccgccgca
     3841 ttctgtgcca gatgatcggt cgctgggtgg aagcgggcga agcaccggcg gacatcaacc
     3901 tgctgggcga gatggtgaaa aatatttgct ttaacaatgc gcgtgactac ttcgccattg
     3961 aactgaacta aggtctgggg ttgatatgca atacatcaag atccatgcgc tggataacgt
     4021 cgcggtcgct ttagcagatt tggctgaagg cacagaagtc agtgtcgata accagactgt
     4081 tacgctgcgc caggatgttg ctcgtggaca taaatttgcg ttaacggata tcgcaaaagg
     4141 ggccaacgtc atcaaatatg gcctgccgat tggttatgca ttggcggata ttgcggcggg
     4201 ggaacacgtc cacgcccaca atacgcgcac gaatctgagc gatctggatc agtatcgcta
     4261 tcaacctgat tttcaggatc tgcctgcgca agcggcagat cctgaagtgc agatctatcg
     4321 tcgcgctaac ggcgatgtcg gggtgcgtaa cgagctgtgg atcctgccaa ccgtgggctg
     4381 tgtcaacggc atcgcgcggc aaatccagaa ccgtttcctg aaagagacca acaacgccga
     4441 aggtaccgac ggcgtgttcc tcttcagcca cacctacggc tgctcacaac tgggcgacga
     4501 tcacatcaac acccgcacca tgctgcaaaa catggtgcgc cacccgaacg cgggcgcagt
     4561 gctggtgatt ggtctgggct gtgaaaacaa ccaggttgcc gcattccgtg aaacgttggg
     4621 cgatatcgat cctgaacgcg ttcatttcat gatctgccaa cagcaggatg atgagatcga
     4681 agccgggatc gagcatttgc atcagctgta taacgtgatg cgcaacgata aacgcgagcc
     4741 aggaaaactc agcgaactga agtttggtct ggagtgcggt ggttctgacg gtctttctgg
     4801 tattactgct aacccgatgc tggggcgttt ctctgactac gtgattgcta acggcggtac
     4861 taccgtactg accgaagtgc cggagatgtt tggcgcagag cagttgctga tggaccattg
     4921 ccgcgacgaa gcaacgtttg aaaaactggt caccatggtc aacgacttca aacagtactt
     4981 tattgcccat gatcagccga tctacgaaaa cccatcaccg gggaacaaag cgggcggtat
     5041 caccacgctg gaagacaaat cacttggctg tacccagaaa gcgggttcca gtgtcgtggt
     5101 tgacgtgctg cgttacggcg agcgtctgaa aacgccaggg ctgaacttgt taagtgcgcc
     5161 gggtaacgat gccgtagcga ccagcgccct ggcgggtgcg ggctgccata tggtgctgtt
     5221 cagtactggt cgcggcacgc catacggtgg atttgtgccg acggtgaaaa tcgccaccaa
     5281 cagtgaactg gcggcgaaga aaaaacactg gatcgacttt gacgcgggtc agctgatcca
     5341 cggtaaagcg atgccgcagt tgctggaaga atttatcgac accatcgttg agtttgccaa
     5401 cggtaagcaa acctgcaacg agcgtaacga cttccgcgaa ctggcgatct tcaaaagcgg
     5461 cgtaacgcta taaagtcgaa atgagttgtt aataaaaaac ggcgtttcat aatgtgaagc
     5521 gccgtttgct tttccatacc tgctaaggat ttatcatgac cgcgtattgg ctggcccagg
     5581 gcgtgggtgt catcgccttt ctgattggta tcacaacatt tttcaatcgt gacgaacgtc
     5641 gcttcaaaaa gcagctttcg gtctatagcg ccgttattgg cgtacatttt tttcttctgg
     5701 gcacctatcc cgctggtgcc agtgccatcc ttaatgccat tcgtacattg attaccttac
     5761 gcacgcgcag cttatgggta atggcgattt ttattgtgct gactggcgga attggcctcg
     5821 cgaagttcca tcatcctgtc gaactattgc cggttatcgg tacgattgtc agtacctggg
     5881 cgctgttccg ctgtaaaggg ctcaccatgc gctgcgtgat gtggttttca acgtgttgct
     5941 gggtgattca caacttctgg gcggggtcga taggcggcac gatgattgag ggtagttttc
     6001 tgcttatgaa tggcctgaat atcattcgtt tctggcggat gcagaaaagg ggaattgatc
     6061 cgtttaaagt agagaaaacc cctcccgcca tagacgaaag gggttaaaca attaattacg
     6121 cagggcgcta tttgccagac gatcgtcttc cgcctggcaa gctgccgcag tgaacagcac
     6181 gtcagttgaa gagttcagcg cggtttcgca agagtcctgc aatacgccga taataaagcc
     6241 gacggcaacc acctgcatgg cgatatcgtt cgaaataccg aacatattac aggccagtgg
     6301 gatcagcagc aaagaacccc ccgccacgcc ggatgcgcca caggcacaca gagaagccac
     6361 tacgctcaac agcagcgctg tgggcagatc gaccggaata cccagcgtat taaccgcagc
     6421 cagcgtcaac acggtaatgg tgattgctgc gcccgccata ttgatggtgg ctcccagcgg
     6481 aatagaaact gaataggtat cgcgatccag attcagcttt tcacacagcg ccatattcac
     6541 cggaatgttc gctgcagagc tgcgggtgaa gaaggcatac acaccgcttt cgcgcaggca
     6601 cagcagcacc agcgggaacg ggttacgacg aattttccac cacaccagca atgggttaac
     6661 caccagcgcc accagtaaca tacagccaac cagcacgacc agcagttgcg cgtagcccca
     6721 cagtgtggag aaaccggtgg ttgccagggt agaagaaacc agcccaaaaa taccgatcgg
     6781 tgcgaagcga atgaccagtt tcaccataaa ggtaacggca ttcgacatat cattgaccag
     6841 gtttttggtg gtctcgttac cgtgacgcag tgcgaagccg aggccgatcg cccacaccag
     6901 aatcccgatg tagttacctt tcagcagggc gtcgatgggg ttggaaacca tgctcattac
     6961 cagtccgcgc atcacttcga caatgcctga cggcggcgaa atatcacccg cgctactgga
     7021 caagtgcagg gtagaaggga aggcaaagct gaagactact gcggccagag ctgcagagaa
     7081 ggtgcccagc agatagagga acaaaatagg acggatattg gttttctgcc cgtgctggtg
     7141 gttagcaata gatgccatta ccagcatcaa caccaggatg ggggcaacag ctttcagtgc
     7201 gccgacgaac aaagtaccta acagaccaac agcttccgcc gcgggttttg agatccatgc
     7261 cagaagaatc cccagaacaa ggccgaccag gatttgtttt accaggctgc catgagccag
     7321 acgccggaat agccccggtg aacgttgcgt agtcattttt cgatcctttc attgtgttgt
     7381 cgcacatccc tggtgcgttc tgtcgacgca tctttcagga tgtaacaaag tgtttgcaag
     7441 gtcgagtata aggaaagtta cagcttcggg aagcgaaaaa tgctggattt taaagagagg
     7501 tcatgttttg caacttaatg gttgcattta tttaacataa acagagggaa ggactggcta
     7561 ttaaggctga ttagataaca tatgggcaaa actatttgga atatgagaac ggttgcgctc
     7621 ctttaacgtg ttatgccata taaagtcact ggcgcaggga gcgcgcaggg ggcggccaat
     7681 cgccgccgcc ccctgccccc ccaggctctg gcgaacaaaa tcgccgctgc gcggttccct
     7741 cagcctatcc cttgcggcta ccgggtcggg cgcaaggtaa cgtccctgta agatgcgccc
     7801 tcgccccggc ctccagggaa cgcttcggcg attttgacgc caccaacacc cgtgcggtct
     7861 ttttgtaaag agctataagc cgattacata attaatagct cacctttgtc tggaatgagt
     7921 tatctctagg aatggcttaa tatacagcga atcattctcc ccgctgctta tcatgccgat
     7981 aattcaccca ggcgttgata ataaacgtca tcaccagaat gccaaacacc acgcccagcg
     8041 agacggcgat tggaatatgg tagaagtcga caatcagcat cttgataccg ataaacacca
     8101 gaatcaccgc caggccatat ttgagcatcg agaaacgctc tgctacgccc gccagcagga
     8161 aatacatcgc acgcaggccg aggattgcaa acaggtttga ggtcagcaca atgaacgggt
     8221 cagtggtcac ggcgaagata gccggaatgc tatccacggc aaaaatcacg tcgctcaatt
     8281 ccaccagaat cagtaccagc atcagcggtg tggcgtacag caatccattc ttacgcacaa
     8341 agaaatgctc gttgtcgatg gtgtcggtca tgcgcaaatg accgcgtagc cagcgcacca
     8401 gcggcttgtc gccaatgcct gattcatctt catgggcgag cgccatcttc acgccggtaa
     8461 acagcaggaa ggcaccgaag atatacagga tccagtcgaa ctgcgaaatc agccagctgc
     8521 ctgtgaagat catgatggta cgcagaacaa tcgccccgag cacgccatac accagcacgc
     8581 gacgttgtaa tgccgccgga acggagaaat agctgaacaa catcagccag acaaagacgt
     8641 tatcgaccgc cagcgatttc tcaatcagat aacccgtgag aaaggccagg gcctgtggat
     8701 cggcaacggc gcgaccttcg gtttgcacca gataccacca gaaagcggcg ttaaacagta
     8761 acgacagcgt cacccagacc agcgaccagg ccgcagcctg tttcatggtc atggcatgtg
     8821 ccccacgacg cccctgcaac aacaggtcga tagccagcat aatggcgaca acaacagcaa
     8881 atccgcccca tagcaacggc gtgccgacag tattcataga agttccttac acataaaaaa
     8941 acggccaacg tcggaagacg tcagccgctg ctttttatgc atagacctcg ccttccggca
     9001 aggtctcact tacaacaaaa aaggaatacg tctgcgtatt cctttcgggt tgcccggtga
     9061 ccggatgtgg tttttcacac atcgtaatga cgatcgaccg gcaatgaagt tactcccctt
     9121 tgcaggtaac aaagtatgac agaccattca gtcagtcaat ggtctgttac atacctttta
     9181 cactgtttta ctttggcaag tatagtttta cgctatctgc cggaaaaatc accccagtct
     9241 ggcggcggat ctcggtcagc agtttggctg tgatgcgact gaccgccagc cctggatgat
     9301 ccaccagatg ctcatccacc agggtagcga acagctctgc ttcgtagagc atggtattaa
     9361 tatgctgcgg ctgggtgaga tcctgcatct ggctgccacg cggcacaaag cagactttct
     9421 agcattcaga cagtttttca atcaccagcg atcccgcttc gccctgaatc tcgcttccca
     9481 ggacagaatc actgaccttt gagtgctgca aggtgacgct gaaatcaccg taatccatca
     9541 ccaccacgcc gtgggcgtca acgccgcttg ccagcaaact cgccgttgcc tgcacgcttt
     9601 tcggctcacc aaataacgcc accgccgacg ccaggcagta aaagccgata tccataattg
     9661 aaccgttaga gaatgccgga ttaaaggtgt tgggattctc accgtccagg taacgttgat
     9721 accgcgagga atactggcaa tagttgaaaa agactttacg cagtttgcct gctttcggca
     9781 gcgcctggcg caacaaatga aagttcggca ggcaggcggt tttaaatgcc tcaaacagca
     9841 ccacctgatt ttcgcgcgca caggcaatgg cggcatccac ttccgccaga ttcgacgcca
     9901 gcggtttttc gcaaatcaca tgaattttat ggctaaggaa aagttgtgtc tgggaaaagt
     9961 gcagggaatt cgggctggca atatacaccg cgtcaatggc atcgctttcc gccatcgctt
    10021 ccagcgacgt aaacagatgc tcgacagaaa aatcattggc gaagtgctga gcctgctcaa
    10081 ggctgcggga atatacggcg gttaacttgt atttaccgct ctcatgggcg gcctcgacga
    10141 actggcgagt gatccagttc gtaccaatca cagcgaaacg tatcataacg cgtggatact
    10201 ccataaaggg attctgtcgt caatgtagca cgcctttttc atcttcgacg tgcgcagata
    10261 cgcattattc tttcgccgga cagccatgct cactaaacgg cccaatcatg accgatggcc
    10321 cgagatattt cggctggcga tggaggattt ttgcgtcaat caccgcgctg acacgagcga
    10381 gtttctcccg cagttgagta taaaaaccat gcttcatatt gaggtctggc gcgttaaacc
    10441 cgatagcgtc gatgccgtac tgttttgcca gccagatggc gcgttggtta tgaaattctt
    10501 gcgagataat ggtgatatgg ttttccccaa acaccttttt ggcacgcact accgaatcca
    10561 gcgttgagaa tccggcatag tcgcaaaaga tcactttcgc cggtacgcct ttagcgatca
    10621 aagcctgctg catacctgat gcttcatcgt aatttttacg tccgttatcg ccactcacca
    10681 gtagccattt cacttttccg gcgtggtaca acgctgctgc ggtatcaata cgtcgggtaa
    10741 agtagcgatt acccggcctc gcccccagca ataaacccac gttacgcgcc gggacggcgt
    10801 tgacatcgcc ccaggtcagc tgcttgctgg cgttgaccat aacgcgatcg gcgataaaaa
    10861 tggtcgctcc tgccacaagt aacagtaagc aggcaatctt tagagtccgg cgagaaaaac
    10921 aaatacggag aagaaggcgg gcaaatgcgc gcagcatggt cgttatcctt aacgaaaggg
    10981 gggaggtcga ttttaggcgc taaagaaaga aaagataacg cctgacatca ttttcagatt
    11041 ttgtcctgcc caagcgacaa ctgcgttagc cacagccggg tgtcgaactc aagctggtgg
    11101 tactgcggct ccatatggca gcacaattga tagaacgctt tgttatgctc tttctccttc
    11161 aggtgcgcca gctcgtgcac cacgatcatg cgcaaaaacg gttccggcgc gttgcgaaac
    11221 acggtagcga cgcggatctc tgcttttgct ttcagcttgc cgccctgcat acgagaaaca
    11281 gcggtatgta acccgagcgc gttttttagc acatggatct tattgtcata catcacttta
    11341 ttgatcggcg gggcattacg cagaaactga tttttcagat cctgagtata ttgccagaga
    11401 gctttatcgg tggcgtagtc gtgtgttccc ggatagcgtt ttgccagcac atcgcccaga
    11461 cgctgttcgt taatcagtgt tcgcacctgc gaaagcagct gctcgggata accctgaaga
    11521 taagtaagat tgctcataac cgcccacgaa aaagagaaaa agggtatact cacgcaccct
    11581 tttcaggggc aacgtcgaaa ttttatcatt caggagggcc gatgagccac ttagacaacg
    11641 gtttccgttc actgacacta caacgttttc cggcgacgga tgacgttaac ccgctacagg
    11701 cgtgggaagc ggcagatgaa tatttgctgc aacagttgga cgacacagaa atccccggcc
    11761 cggtgttgat cctgaatgat gcctttggtg cgttaagctg cgcgctggcg gaacataagc
    11821 cgtacagcat tggcgactca tacatcagtg aactggcgac gcgcgagaat ttacgcctaa
    11881 acgggattga tgagtcgagc gtgaagtttc tcgacagcac cgccgactac ccgcaacagc
    11941 cgggtgtggt gctgatcaaa gtgccgaaaa cactggcatt gctggaacag caactgcgtg
    12001 cgctgcgcaa agtggtcacg tcggatacac gtattattgc cggtgccaaa gcccgtgaca
    12061 ttcacacttc cacgctggaa ctgttcgaaa aagtgctcgg cccgaccacc accacgctgg
    12121 catggaaaaa agcgcgcctg attaattgca cgttcaatga accgccgctg gccgatgcgc
    12181 cgcagaccgt tagctggaag ctggaaggta ctgactggac tatccataac catgcgaatg
    12241 tcttctcccg caccgggctt gatatcggcg cgcgcttctt tatgcaacat ctgccagaga
    12301 atctcgaagg ggagattgtc gatctcggtt gcggtaatgg cgttattggt ctgacgctgc
    12361 ttgataaaaa cccgcaggcg aaagtggtgt ttgtcgatga atcgccgatg gcggttgctt
    12421 ccagccgttt gaacgttgaa accaacatgc cagaggcgtt ggatcgcagc gagtttatga
    12481 tcaacaacgc gctctccggc gtggagcctt tccgctttaa tgctgtgctc tgcaaccctc
    12541 cgtttcacca acagcatgcg ctgaccgata acgtcgcctg ggagatgttc caccacgccc
    12601 gccgctgcct gaaaatcaac ggcgagctgt atatcgttgc caaccgccat ctggattact
    12661 tccataaact gaagaaaatt ttcggcaact gcaccactat tgcgacgaat aataaatttg
    12721 tggtgctgaa agcggtgaag ctgggacgtc gtcggtaaga ttgggagtta cctgccggat
    12781 gcggcgtaaa cgccttatcc ggcctacgtt cagcacgtta cccccaggcc ggataagacg
    12841 cggcaagcgt cgcatccggc aatgtctgct cagttctgga atcgcgcttc cataaaacaa
    12901 aaaatccctg tcaccatcct ctacattctc ttgtttagcg tttttctacg tttattcttc
    12961 cgtcacacag ataaattcat ccgttgccaa tctgtcaacg gatgttatca tattgccaat
    13021 ttgtcaacgg acgtgatgaa gaatgcacct gataactcaa aaagcattga aagatgctgc
    13081 ggaaaaatac ccgcaacata aaacggagtt ggtggctctg gggaacacga ttgctaaggg
    13141 atatttcaaa aaacctgagt cattaaaagc agtattccca tctctggata acttcaaata
    13201 tctggataag cattatgttt tcaatgttgg gggcaatgaa ttacgtgttg tagcaatggt
    13261 cttttttgaa tcgcaaaagt gctacatacg tgaagttatg acgcataaag aatacgattt
    13321 ctttaccgct gttcatcgta ctaaggggaa aaaatgattg cgattgccga catcttgcaa
    13381 gcaggagaaa agctaactgc tgtggcacct tttctggcgg gtattcagaa cgaggaacaa
    13441 tacacccagg cgctggaact ggtagatcat ctgctgctca acgatcctga aaaccccttg
    13501 ctggatctgg tgtgtgccaa aataaccgcg tgggaagaat cagcgcccga atttgcggaa
    13561 tttaatgcca tggctcaagc catgcctggc ggtatcgccg tgattcgtac ccttatggat
    13621 caatatggtt taaccctttc cgatctgccg gaaattggca gtaaatctat ggtgtcacgc
    13681 gttttgagcg ggaagaggaa attaacgctg gaacacgcta aaaaattggc aacgcgattc
    13741 ggcatttctc ccgccttgtt tattgattaa cgcgacgtgc cggatgcgtt gcatccggcg
    13801 cttcaaataa cgatttaaat ctccagcgcc agccgtgttc cctgggcaat tgcccgtcgt
    13861 gcgtccagct ccatagccac atcgcagccg ccaattaaat gcacagtttt cccgctatca
    13921 atcagcggtt gcgccagcgc gcggtttggc tcttgccctg cgcagatcac cacattgtcc
    13981 actgctaata cctgcgtttc gccgttgatc accacatgca gcccgtcatc gtcaatcttc
    14041 tgataactta cgcctgggat cattttcaca ccccgcgaga gcagggtggt gcgatggatc
    14101 cagccggtgg ttttgcctaa cccctgtccc ggtttgctgg ctttgcgctg gagcatcaca
    14161 atttgccgtg ggctacgcgg gatctgcatt ccctgcgggc ttaagccacc agcctgttgt
    14221 aggctgctgt cgatccccca ctcattacag aacctggcga tattctggct ggtggattcg
    14281 cccggctgac ttaaatacat cgccgtatca aaaccaatcc cgccacaacc gatgatggca
    14341 accttgttgc ctaccggcgc tttgtcgcgc agtacatcga gataactcaa taccttcgga
    14401 tgatcgatcc cgtcgatagg cggaacacgc ggcacgatcc cactggcgag gatcgtttca
    14461 tcgaacgcct gtaactgatc cgccgtcacg gtgtgattga gtttcagcgt cacgcccgtc
    14521 acttcgatca tccggcggta gtagcgcagc gtttcgtaaa actcctcttt gccggggatc
    14581 tgtttggcga tattaaactg cccgccgatc tcgctgtgcg catcaaacaa tgttacctga
    14641 tgcccacgcg ccgccgcgtt aatggcaaac gccagcccag caggtccagc gccgaccacc
    14701 gccagatttt ttttctgcac ggcgggaagg attggcattt tggtttcgtg gcaggcgcga
    14761 ggattcacca ggcacgaggt gactttgcca acgaagatct gatcgaggca ggcctggttg
    14821 cagccaatac aagtgttgat ctcatcggct cgccctgatt gcgcttttga cagcagttcc
    14881 gcatccgcga gaaacggtcg cgccatcgat actatatcgg catcgccgcg cgagagaata
    14941 tcgtcggcaa cctgcggatc gttaatccgg ttggtggtta ccagcggcag cgagacgtgg
    15001 cctttcagtt tgcgcgtgac ccagctaaat gcgccgcgcg gcacgggcgt ggcgatggtc
    15061 ggaatccgcg cttcgtgcca gcctattccg gtgttgataa tggtcgcgcc cgccgcttca
    15121 atggcctgcg ccagttctac cgtttcggca aaagtgccgc cgccttcgac caggtcgagc
    15181 atcgacaacc ggtaaataat aatgaagtcg ttgccgacgc gttcgcgcac cgtacgcact
    15241 acttctacgg caaatcgcat ccggttgcgg taatcgccgc cccactggtc actacgctga
    15301 ttggtgcgca gcgtcagaaa ttcgttgatc aaataccctt cagagcccat cacttcgacg
    15361 ccatcgtatc cggcctcccg cgccagttgc gcgcagcggg cgaaatcgtc gatcagttgc
    15421 aggatctctt catgggtgag ttcgtgagga acgaaacggt tgatgggggc ctgcaatgcg
    15481 gacggtgcga ccagatgcgg ttggtagctg tagcgcccgg tatgcaaaat ttgcagggcg
    15541 attttgccgc cttcctgatg tacggcttcg gtaatggtgc gatggtgtgg gatctggctg
    15601 gcgtcgttga gcattgcgcc accttccatg ccaacgccta ttaaatctgg tgcaatgccg
    15661 ccgctgacaa tcagcgccac gccgtgacgg gcgcgttcgg cataaaacgc agccagccgt
    15721 tcagcaccgt ccgggtattc ctccagcccg gtgtgcattg aacccatcaa cacgcggttt
    15781 tttaacgtgg taaaacctaa atccagcggg gcgaacagcg acgggtagct cataaaattg
    15841 tccagtatgt aaaataattg ttacggatga gttctaattt agccgtcgat ggagaaaagg
    15901 gaaaagggga gtgcggggtt tgtgatggga ttcaaaaaga atacgcccgg tggtaacgcc
    15961 ggatgcgacg cttgccgtgt tttatccggc ttgggggatt gcacatgtag ggcggataag
    16021 gcgttcacgc cgcatccgcc agccgttgca aattactgct tacggaaaaa atcgttatac
    16081 aacatataca aatgagcggc actccaggag aaatttggtg cgccttgctg cgcgcctgtc
    16141 agcgggttgt aattctcctg aattgggcca tcggcggtta atcctttggc gtgcctgaag
    16201 aacgtatccg ccagcttcag ggcatcatca cgataaccgt aacgctccat ccctttcaga
    16261 ccaaaccaga actgatccac ccatacgcgc ccgcgccagt agatatcagc gccaaaagcc
    16321 ggattggtta acgccgccgt tcccagcgga acaaaggtgt tgaactcttt aggatcgagc
    16381 atcaccttca ccaccgcgtc ggcattggcc tgcgttgccg caccgttaaa cagcggcgac
    16441 cagccttccg gccctttacc gcgctcaaca atcggtttgc ccgcgcagcc gttcgccagt
    16501 ggtttatctt caatacgtac gtcatagtag aactgggtag tcgggtcgaa catacaggtg
    16561 ttgatgtagt ccgcgagctg ctgtgccaac tggcgatagc gtttggcctc ttctggctta
    16621 ccgaggatgg ttgccatctc cgccagataa tggttatcgc tgtacatata gctggcctga
    16681 tccaccgact cctgcaatag cgagtagccc agcaacgttc cgtcctgact gcggttttcg
    16741 gcgaatttca ccgtctagtc gctacgtttg ccgccattag cgacatattt atccaactgt
    16801 tctttgtcga taaaaccaaa gacggcggca tcatcacgcc ccgattccca cgacgcagcg
    16861 acctgtgccg gaatttccag actgtcgtac tggcctttct ccaccacgcg ggcgtagttg
    16921 ttcagcccag actgcgtctc ttctttatcg ccttttttca ccgtaaacag catctcgccg
    16981 ctctcggtgt tgtgggcttt gtcgcgggtc gcgccatatt ccggcacccc gttgccgtta
    17041 tgatcgcggt tacgtaacca ccagtcgtga taggccacca gtttcgggta catctctgcc
    17101 aaccaggctt tatcctgggt gacgttgtat acttccatca ccgaccaggc ggcaaggctg
    17161 ggtttggtat tacgttcgtt ccagttgccg ccatcgccac catgctcggg gctaaggttc
    17221 cacgctatca ggtcggggac aaagcccaca tcctgcggac gcacgctgtc gccaggctgg
    17281 atctgccagg agaagaccgc gcggatattc tctttggcga tatccggatt gaaatgcgcc
    17341 atcgcaaacg cctgcttcca ggtatcccac ggccaggtct gattgccgga gaaccagcgt
    17401 ccggtcaccg acggcgtgac ggtgttataa ttcaccgcac cgcccggtga gcgccagtta
    17461 ccgttgagcg tttcgatcgc tttcaccgcg acgcgcgtct gttccggcgt cgcatccgga
    17521 ttggttaacc ctttcttcag gtattcttcc cagcgttgct gcgaggcggt gagataaaac
    17581 gccggacgcg ccagaatatc gcggatctgc atttgctctt tgctaacttc ctgagcggtc
    17641 agcagatggg aataggtggt gtagagcgtg gtcgaaccgt tgatatgcgc cttactggta
    17701 aagcgattac cgttgatttc agtctgcacc ggcagggatt tatgcacctg atattccgat
    17761 tcgccggagg tcagcagatc ccaggtggcg cgcactttgc caaaggtgac tttcaggcca
    17821 tcacgggtgg cgctgatttt gcgctgatag tcagggtatt cgccagcaat ggttttatcg
    17881 gaaagcggtt tcccttcttt cgcttccagt ttttccagca gttcgccatc ccacaccaga
    17941 tccagcggtt tattgctgat gattttagtt tccagtagtg acgtgcgcgg cgtggcgaag
    18001 cgcagagtca tttcgacctg cacatctttt gctgtcagtt tttgcaccag cgcgccggga
    18061 atactgtatg cctccagcgt gaagttgact ttcttgccgt cttgccagac agtcaggcgg
    18121 tcgaaattgc tggccataaa gttgatgtat tcttccgtca gcagcgcaac gcccggaaag
    18181 ccgcccatgg tgttcgggcc gtctggcaac agatgaccat gccaggctcc gagatcgaaa
    18241 aacggattaa aacgctggtg atcgtcgtaa tcgtaatcct tcatgtactg cggcgcacca
    18301 gtacggttaa tcacgttttt ataattgtcg gcgttagcgg catgagcgga aaaacttatc
    18361 agcagagcgc aggttactgg cgttaaaata gttttgattt tcatgaaatt acagtcctta
    18421 ttaccagtag aaatattgca tcaggaagat ttggtcgctg ggatcgttag aggcattttt
    18481 catgtagaag cggctatcaa aagccgtggc gaaacgtccg ccgccatatt cgtaccagat
    18541 accaaactgc atgaaagagg tggtttcatc gtcagcgtgg tcagggcgat gtttggcgta
    18601 ggtgtaagcg gtggagagat aaacgccctt taacgcttca tacatggcgc tcaccatcat
    18661 cccgctttca cttcccggta cgtccagccc gtcggcacgt ccggtatggt gccaggcgcg
    18721 agcggcaaag ttaggggtaa acaggcccgt gagatagagt tgtcctgtgc cgtcggtaat
    18781 ttccagacca ttcatccagg tgatgccttc ggcaaggtca tactggatgt aaccgttcat
    18841 ccattgccgg ataggtgtat ttgtcgtcgt agcgatcgta cttgccaaaa tgtagccagg
    18901 ctttgctttc gtcatgatgt ccggcaggtg tggcggtgac gctataacga aagttgccgc
    18961 ttaagttctg taccttcagc gcggtcataa tgtcgcgggt attgggaatg acgtagccca
    19021 aatcgggtgt gaagtcgccc caccattgca ggtcatccag cgaggaatcc tgacgcagac
    19081 tgagcatgat ttcggtgcca tcattggtac gataaccgcc atagaaacgg ttaatcccac
    19141 cttcgaaacc accccagcta tggtcaggca cctaggcatt accgtcgtgg tcggcctgca
    19201 cggtccagcc ttcgccgtaa atcaggccga accaggagcc gtacttcatc tccaggccac
    19261 cttcaatata ggtgccgtcg ctgtatttat gtttattctc accctctaaa tccatatatc
    19321 caccaagccc taattcgccg taaaaacgaa atgaaggaga agatgtcgag gtaattgcgg
    19381 gtgtttttgt gtcgtcatgt tctgccggac gtgcttcttc tcctgccgca gaaaaggcgt
    19441 agcaaaacgg cagagcaagt aatgctctgc atggtgcagt gattaatttc attgtgttaa
    19501 tccatgagct aaagaaaaag tacagccttt tatcgttgta attataaaaa tataaaggca
    19561 gaaactattt tccctctcct catccaggaa gagggaaaat gcctgctcat tttttattag
    19621 tctgcttaat caacattgct ggctggcgtc gcttcaatat ggcgctcttc agccgttaat
    19681 ccctttatat atttactgta tttccaccac gcaaagccga ggaagataac aataccgccg
    19741 acgttataaa aaatgatggt cagaatattc gcgccagtcg ggaatgtcga agcgacaaac
    19801 cctacggcaa atatcgcaat cagcattgaa acaacgataa taccggtgcg gcgggagccc
    19861 atacggaaat cgcgtggcag gtgatctaat ttggcgcgta aattcaggta agccagcatg
    19921 atgaataacg gcggaagcat ggacgctgcg gcggtcatat taataatggt attcatcaga
    19981 tcctgcacgg tattggaacc gagcatcggg ataatcatca gcgggatgac gatcagaaac
    20041 tggatccatg ctgcgcgcgc cggaacgccg ttttcgttaa gttcgacggt tttcttacca
    20101 aagatgcctt ccgggatttc ggagaagaaa attttcaccg gcgttgcggt ccacatcagc
    20161 agggaaccga acattgcggt aaaggacacc agaccgacaa agcgattcat caacgcttcc
    20221 ggcagaccaa aatacgccgc catgccgtgg aatacctgca ccgatccgcc ggtaaatttc
    20281 aactctttgc tgctgacgaa gacgttaatc agcaccgagg agacggaata cagtacgccg
    20341 ataaaaatcc cggcgaggat gatcacttta acgaacgatt tcgaaccgcc tttgacgtcg
    20401 ttaacgtaca ccgcgacgga ctccgcgcca cctgcggcca taaagatcca ggtggtaacg
    20461 ccgaggaacg cccagttgaa gttcgggatc atcgcgtcaa cggtgatcgg atcggcaggc
    20521 tgtacgtcgc caaccagtgc cgtacctgcc agtaaaatgt aggagagcgt taacagcagc
    20581 atcagcgttg aagtgacgga ggtaattggc cccagcattt tcgccccgtt ggtggaaacc
    20641 caggtggaga aggcgaacag caccatactg ataacggtgg tggcaaccgg cgtcataata
    20701 tattcgtagc cgaggaaggc atacgaagca taagcaataa cgcgcggcaa cagcgaggtg
    20761 aaaaagaaca gatttacgaa ccagtaagtg taggcagtaa taaatgccca acgtccgccc
    20821 agcgaacttt ttacccacgc gtagacaccg gcttctgagt ttttatttaa cgaaacaaat
    20881 tctgcgatga tcagacagaa gggaataaaa taaaaaatcg tcgcgaggaa aaacatcggt
    20941 gccgaggcca gtccaagctc aatattatta ttgataacgt tgttaaagct gtaaacggcg
    21001 gcaaaagtca gcgagagcaa gccgaatttg ccgattgtat tacgtttggt atcagacata
    21061 acacatctcc gaaaatgtgt agggtagcgg tggcggcaaa ctccttttgc cgccgtagtt
    21121 gttatttgtt atggaaataa ccatcttcga tggtgacttt gagaaccact tttttgaccg
    21181 cgttattgca gataaaccga tacgcttcat ggatatcgca aataacgatt tgcccttcgt
    21241 ggacctctac ggtttctccg cagcctttta aatattcacg gtcagtttca tcacgataat
    21301 attccactac ctgtaatgtc tctttcggcg catattcaat tttttgctgc ccttgcaggt
    21361 agtaatgcac ttcaaaatag cgtcgatgcc cggtaaatag cgcatgggtc gcggagtcat
    21421 tttccacacg gtaggtcaat gaatcaccga tggagtgggc gacgccaggc tgaatgttgt
    21481 cgatattttc aatcgcttca acgcagcgtt gccacttctt gccagaggcg taaatctggc
    21541 ggaactgttc taagttatcg atgatcctca ttgcttattc tcgctgtgca aattcgtgga
    21601 aaagaaccct gcgccgaact catacgacgc caggctttgc gcggtagctt ctccgccaga
    21661 aaccggcagc aacgtaaagc cgtagctgaa gtcacggaac cagacgcgcc aggagtccag
    21721 cacctcgctg ccccaggagt tggagccgag gccaagtagc tggtggtcga ggttcagggt
    21781 gatgtcatca ctgcgctgca gctcgttaca gtgctgggca gcgtggatgt tttcctgggt
    21841 atagtgccag gcgctgaagt taattgggcg ctgcggaacc accagcagac cgttgtcgtg
    21901 gcggttagtc agtgccgtcc agcggacatg ctgacggttg ccgttgttct gcgggaaggg
    21961 atagttctcg aacatggcat cgacggtgct gcgccagata tcgatgatgt tagcctgctg
    22021 gctgtcggcg tagttttcgc ccggtccacg accgtaatac gccacctgat cgtattcgcc
    22081 gttaatcccc atggtgaaac cgatgcacgg aatgatgtgc ggatagtcgc cgtaacgctc
    22141 gccggaaagc gccacgttaa cctggccatc ggcagcgatg cgccagatgt aggtacagcg
    22201 catcccgaag tcaaacaccg gcggtgcaat aactgtgcgg ctgatgatca acacttcacc
    22261 atcgctctgt tctacggcaa agtcgcgcag atgttcctgc atgatctgca aatgattcgg
    22321 ctgccacaac ccttcgtact cctgcttgtg gttgtcgatc atcggcttga agaagttgat
    22381 ctttggctcg cgggtcagca gcgactcgcc atttacctgc caggatgtcg gtttgccact
    22441 catttttgag aaggtgatcg cgaagttgta gccgcgaacg gtgcagctca aacgatcgtc
    22501 ttccagcgtc agcggacgcg cattatttgg tgcgaaaggc actggctgcg cggtgttttc
    22561 cttcagcggg aactgataag tggcgataga atgcccggct tcgctgtagc gggtgcggga
    22621 atctttggtc accgtaatgt tgaggaacgc ttcgcgggcg tccagctgcg gcagcgtgat
    22681 ctgcaagggg gcttcgctgt tcggcgcaac gtcgcgcagt ttaatctgct gcgtcgcgag
    22741 cgtttcacct tcggcgcgca cctctgcgtg cagggtgtag tcatcaagcg tggtaaacca
    22801 cagtttattt tcgactttca actcgccgcg agtcagcgcg tggattttta ccggcgcgat
    22861 aacctgtttg tactctttca ggcccgatcc cggcgtctga tcggaataga tcaaaccatc
    22921 aagacagaag ttatagttgt tgggatagtc gccgtagtcg ccgccgaatt tataccagac
    22981 attgccgtgg tcgtcctgtg cctggatccc gtggtcgcac cactcccaga cataatgacc
    23041 ctgaatgcaa tcgtgcttat agaagacgtt ctggtactcc gtcagcccgc ccggtccgtt
    23101 ccccatcgca tgagcatatt cacagatgat gcgcggcttc ggatgcgggt attcaccaaa
    23161 ctcattcatc agcggcacgc gggtgtacat ggtggaaata atatcgacca cttcagcatc
    23221 gcgatcttct tcgtaatgca ccagtcgcgt gtcatccagc gctttcgccg catggtacat
    23281 cgcgcggatg ttacagccat agccggattc attgcccagc gaccagatga tgatcgacgg
    23341 atggtttttc tgtgcgtgga tatggcgaac aatgcgctcg acgtagactt tttcccactg
    23401 cggatcgtcg gtaatacggc tgatatcgcc gacattagca aagccgtgcg attcgacgtc
    23461 ggtttccgcc atcacaaaca ggccgtagat atcacacaat tcgtaaaaac gcggatcgtt
    23521 cgggtagtga gcggtacgca cggagttgat attgtgctgc ttcatcaact ggagatcttt
    23581 ctcgacgcga tccattccaa cggcgcggcc tttgcgatga tcgttgtcgt gacggttgac
    23641 gccgtgcagc atcacataac ggttattgat ccagaacaga ccgtcgcgca ctttgatatc
    23701 acggaagcca acgcgttgtg gcaccacttc cagaacgttg ccgttggcgt ctttcagcgt
    23761 catgaccaga tggtaaagat aaggggattc tgctgaccat tgctgcggct gttcgacagt
    23821 aaaagcaaag ctggcgctgg tcagtttttc aattgccaaa tgatcaatgg cgctgctgtg
    23881 caccacgcgt tcgccatcaa acagggtata ttccagcgtc gtgacgacag gggaggcggc
    23941 gagattttcc agcaccactt cgcaggaaag cgtggcatcg caataggctt cgtcaaagtc
    24001 ggtacgcaca gtgaaatcgt taatatgcgt taggtgtttt ccgaccagat aaacatcgcg
    24061 gaagatcccc gccgaccacc acatatcctg gtcttccacg taggtagagt ccgcccactg
    24121 catcacgcgc acacacaaca ggttgtcgcc ggttttaacc atcgcgctga tgtcaaactc
    24181 tgcggtcagg cgactgccct tgctgaaacc cacatactga ccgttaacgt agacttcaaa
    24241 ataggtttcg acgccgtcaa atttaatcag cgtctgttta ccctgccagc cgtcgctgag
    24301 ggtgaaaata cgttgatagg caccggttgg gttatcgctg gggacaaacg gcacatcgat
    24361 ggggaacgga aaaccttcgt cggtatattg cagtttgccg tgaccttcca tttgccacat
    24421 ggcggggacg gtaatatgcc cccagtcagc cattaactca gaggtgaagg cttctggtac
    24481 ttgcagcgga tggtcaaaaa agtggaaatt ccactgaccg cttaagggca gaaacaggct
    24541 gctggtttcg cgggcaaagg tacgcgcttg cgcaacagaa tcatatgaaa aaaagtacgc
    24601 acgcggcgca agtcggtttt cgtgggtgag ctgaatgttt tcccagcgat tcataggacc
    24661 tcccggtaga gagaaatcgg aaccattcat ggtgatggta gtttgtgtaa ttgctggggg
    24721 ggttagtaaa atttttacta aaagtagcag gtaaatgcat ttactttaac tggatcacga
    24781 aaataaacgg cagcactgtc cggcaaaaat gattgccgga cagtgtgtaa ggggatttaa
    24841 cgggtcgtgc cgcgcagttt taatttgctg ggaacgaaga ctagcagcgg cagcgcgcga
    24901 ccatcgcggg ctttttcata caccaggtta acgccctgac ttcccatcat ctcggaatgg
    24961 atgcgcacgg tggagagcgg cggaaaggta aatcgcgcgg tggggatatc gttaacgctg
    25021 ataagcgaaa tatcttgtgg gatgttcagg ccacgttcat gaattgcccg cagtacgccg
    25081 atagcaatgg aatcggaagc aacaaacagt gccttcggat agtcttcccg cgccagcatt
    25141 tgttttgcca gttcataacc cgacgaactg gaaaaaccgc cgcgccagat atcctcttcg
    25201 cgtaccactt gtttcagtcg gccatattcc gcaaaggcga cctcacgaat atccgccttg
    25261 ccaggctcat cttcaccgcc aataaaacca atacgattaa cgccctggtt gatatagaag
    25321 tcgatgattt ctttactgat gcgtgccaga tcgatatcca ccgcatcgta accgctgccg
    25381 ggttcgtgaa agtcgataaa acagatattg tcggtcaacg cgctggcagc ggcgcgcagg
    25441 gcgggcgtgg gtttgccgac aattaaaata ccggtgacgt ttttaatgtc tggtaatccg
    25501 ttgtgttcat aacagttggt cagttcgatc gccagctttt cgcactgagt ttcaatgccg
    25561 tggcggatcg ccagatagta aggatcgttg atctccagct cctgctggta gctgtagata
    25621 gccagaatat ggtgttggtt gactgcgcct gtctgtagtt tacgggcgct actggtcttg
    25681 tactccagct tttcggcgat ctcgagaatg cgatgtttcg tctcttcttt cacattcaat
    25741 gtcggatcgt catttaagac cctggatact gtcgccaggg atacgccagc ttcgattgcg
    25801 atgtctttta atgttgccat gtttaccttt tttactttcc ctgatgtgcg cccccattgt
    25861 agaggaagtg cttatcgggt cacctgtttt cagtaaataa agcgcgtttc tttgacgctg
    25921 taacggtaac acagcggctt aggaatgtga tcacgcctgc attctccatt tttagcctac
    25981 aggattgaga aaaacttttg ctatagttta gtaaaatttt acccgaaagg aggaagcaat
    26041 ggaaaccgtg gcttacgctg attttgcacg tctggaaatg cgcgtcggaa agattgtgga
    26101 agtgaaacgc catgaaaacg ccgacaagct gtacatcgta caggttgatg tggggaaaaa
    26161 acactgcaaa ccgtgaccag cttggtgccg tactacagcg aagaggagct gatggggaaa
    26221 acggtggtgg tcttatgcaa tctgcaaaaa gcgaaaatgc gtggtgaaac gtcggaatgc
    26281 atgctgttgt gtgcggaaac cgatgacggc agtgaaagcg tgttgttaac gccggagcgg
    26341 atgatgcctg cgggcgtacg cgtcgtataa aaagatcgga tggcgacgtc gtatcgccat
    26401 ccgatttgat attacgcttc ttcgacactt actcgcatgg ccgccagcgc cttacgcgcc
    26461 gctttgatca ccagttcaca ctgttcaatg gtcagtgtca gtggcggttc aatgcggatc
    26521 gttttggcgt tattgagcgt tccggccacc agtacgcgct ggcggaacat ctcgctggca
    26581 aagttatagc cgatttcgtt atcaacaaac tcaatcgcca tcaacatccc tttaccacgc
    26641 gcttcctgta ccagatcggg atattcccgc gccagttgac ggaaaccgtc cagcaacata
    26701 tcgccttttt gctcagcctg agccggtaag ttctgctcca gcaacacatt gatggtcgcc
    26761 agcgccgccg cacaggccag cgggttgccg ccaaaggtgg tggtatgcag gaatgggttg
    26821 tcgaacagaa ctgaaaacac ctcttcagtg gcgatggtcg cgccaatcgg catcacgccg
    26881 ccgccgaggg cttttgccag acacagaatg tccggctgaa cgttctcatg ttcgcaggca
    26941 aacatcttgc ccgtgcgccc catgcccgtt tgtacttcat cgaggatcat cagtgcgccg
    27001 aactcatcgc acagcttacg cacggcggtg agatagcccg gcggcggcag aattacgcca
    27061 ccttcgccct gaatcggttc gaggatcacc gcagccacat catcaccggt ttttttgcac
    27121 tcattaagag ccgtgcgcat ggcttcgata ttgccaaacg gcacatgacg gaaccctggc
    27181 agcagtggca taaacggttt gcggaaggtc gatttcgccg tggccgacag cgcgccaagt
    27241 gatttaccgt ggaacgcgcc gctggtggca ataaaagtaa atttgccgcg cggcgactga
    27301 taagccttcg ccagcttcag cgccgcttca acggactcgg tgccgctatt acagaagaag
    27361 ctgtatttca gtttaccggg cgttagcgca gcaagggttt tcgccagcat agcccgtagc
    27421 ggatcgagta gctcctggct gtgcagtggt tgtttcgcaa gttgattctg tacggcggaa
    27481 accacaactg gattacggtg ccccacgttg aaaattccaa aacctcccag gcagtcgata
    27541 aactcctgtc cttgggtgtc gacaagcgta tttaaacctc ccgcttgcca ctctacggct
    27601 ccgtaatccc cgccggcggt aacagatttg cgatactcta aaaaccccgg attgacatgc
    27661 tctttgaagt attcaatcac ctctcggtta agtgctttca tctcctcatg atccagcgtt
    27721 cgcttctcaa tgagattcag ggcgtgggcg ctgcacgcta aagccgatgc gctcgaaggt
    27781 aacctgttca aaatatgctc cggaggctcg cgtatcacat gataccgatt taagtattgc
    27841 agggattgcg ccactccggc tgcgatcgcg aaaatcggga taaacacaaa tgcggttatc
    27901 atttgtgcaa tatttaatgc tatccgttaa catttgttgt tattttgcac ggttttgtcg
    27961 ggtatggatt tgatgattgc tgcgcaaatg cacgttttca gggcaataat tgcctgattt
    28021 ttgggcaaga taattaacgt tttcgtatag taacagagtg aaagtatttt aaatcaaaga
    28081 attagatttg tgctttaatc gcaaattgcg atctaaatca aattaatcgg ttaaagataa
    28141 ccgcagcggg gccgacataa actctgacaa gaagttaaca accatataac ctgcacagga
    28201 cgcgaacatg tcttctcatc cgtatgtcac ccagcaaaat acgccgctgg cggacgatac
    28261 cactctgatg tccactaccg atctgcaaag ctatatcact catgctaatg acacttttgt
    28321 gcaggtgagc ggctttacct tgcaagagtt acaagggcag ccgcacaaca tggtgcgtca
    28381 cccggatatg ccaaaagcgg cgtttgcgga tatgtggttc accctgaaaa aaggggagcc
    28441 ctggagcggc atcgtgaaaa atcgccgcaa aaatggcgac cattattggg tgcgggccaa
    28501 tgcggtaccg atggtgcgcg agggaaaaat cagtggctat atgtcgattc gtacccgggc
    28561 gacggatgaa gagattgcgg cggtggagcc gctgtacaaa gcgttgaact ccggacgtac
    28621 cagtaagcgt attcataaag gcctggtggt gcgtaaaggc tggctgggta aactgccttc
    28681 attaccgctt cgctggcgga cgcgtggagt gatgaccctg atgtttatct tgctggcggc
    28741 catgctttgg tttgttgctg ccccggtggt gacgtatttc ctctgtgtgt tagtggtatt
    28801 gttggcaagc gcttgttttg aatggcagat tgtgcgcccg atagaaaatg tcgcccgtca
    28861 ggcactgaag gtggcgaccg gagagcgtaa tagtgttgag cacctgaatc gcagcgatga
    28921 gctggggctg acattacgcg cggtagggca gcttggcctg atgtgccgtt ggctaattaa
    28981 cgatgtctca agccaggtgt ccagtgtcag aaatggcagt gagacgctgg cgaaaggcac
    29041 cgatgaactg aacgaacata cccagcagac agttgataac gttcagcaaa cggtggcgac
    29101 catgaaccaa atggcggcgt cggtgaaaca gaactctgcc acggcgtcgg ctgccgataa
    29161 actttctatc accgccagta atgcggcagt gcagggtggg gaggcgatga ccacggtgat
    29221 caagacaatg gacgatatcg ccgacagtac ccagcgcatt ggcaccatta cttcgctgat
    29281 taacgatatt gcgtttcaga ccaatattct ggccctgaat gcggcggtgg aagcggcgcg
    29341 tgccggcgaa cagggcaaag gttttgcagt ggtggcaggg gaagtgcgtc atttagccag
    29401 ccgcagcgct aatgctgcca acgatattcg caagctgatt gatgccagtg ctgataaggt
    29461 gcaatccggt tcgcagcagg tacacgccgc cggacggacg atggaagata ttgtggcaca
    29521 ggtgaaaaac gtcacccagt tgatcgccca gattagccat tcaacgctgg aacaggccga
    29581 tgggctttcc agcctgaccc gtgcagtgga tgagcttaac ctgatcaccc agaaaaatgc
    29641 cgagctggtg gaagagagtg cgcaggtgtc ggcgatggtg aaacaccgcg ccagccgact
    29701 ggaagacgcg gtgacggtgc tgcattaatc ggtatgccgg atccggcggt tggagcacaa
    29761 tgcctgatgc gatgctgacg catcttatca ggcctgcggg gtgtgtagcg ggtgtaggcc
    29821 ggataaggcg cgtcaagcgt cgcatccggc aattgcaccg cgccactggc ggatgcggcg
    29881 attaatccag ttgcgtaata tcaaaagcgg cgcggtcgat caccgcaatg atctttttta
    29941 tttgtgcatc actgatatcg ctctggttga cgcgtaaatc cagcaccgct ttaaaattat
    30001 ccagcgcccg cttcatttgc gggttctggc gcagcgccgc gccaacgcaa cgcgctttga
    30061 tgcgttcttc aatcatctcc acctgttcgc ggttttcttc cagccactgc gcgccttgtt
    30121 cggtcagcgc aatctgcttc ttaccacctt cctcttcgcg gatggtaatc agcgactgct
    30181 cctgcagaaa atccagcgtc gggtagatga cgcccgggct tggggtgtaa ttcccctggg
    30241 ttagattctc aatcgcttta atcaattcgt aaccgtggct gtcatcgcgc gagagaatat
    30301 ccagaatcac cagacgcaat tcaccgtgac caaagaaacg ttgccgacga ccgccgccgc
    30361 gaccgtggcg accaccgcaa cattgaccgt gttcatgctg gtgaccgtgt ccgcagtgct
    30421 cgtgttctga cttctcacct ttgcagcagc cctcatggcg tggctggcct tcatgtttac
    30481 aacacccttc gtgatgatgg ctcatatcag cctccttttg cttgattaga tatatctaat
    30541 ttatatctga tgtgagcatt actgcaagta caaaaatgat ttttaaacgt ggaagcacct
    30601 cacatctagg ccggataaga cgttcacgcc gcatccggca gcattgctat ggcactgaat
    30661 gccttcacat acttcagaca tatcagttct acaaatcgct tgcatttatc atgattaaca
    30721 atcattatca tttgcgaatt ttatttagat atatctgatt accatcacga aggcgataac
    30781 aatgaataac accccccgct acccgcagcg cgttcgcaat gatctgcgct tccgtgaact
    30841 gactgtgcta cgcgttgagc gcatcagcgc cggttttcag cgcattgtcc ttggcggcga
    30901 ggcgttggac ggttttacat cgcgcggctt tgacgatcac agcaaactct tctttcctca
    30961 acctgacgct cactttgtgc cgccaacggt aacggaagag ggcatcgtct ggccggaagg
    31021 accacgccca ccgtcgcgtg actatacgcc actgtatgac gaactacgcc atgaactggc
    31081 gatcgatttc tttattcacg acggtggggt cgccagcggc tgggcgataa acttacggtg
    31141 gcaggtccgc gcggttcgct ggtggtgccg gaagattacg cgtatcagct gtatgtctgc
    31201 gatgaatccg gaatgcccgc actgcgccgc cgcctggaaa cgttgagcaa acttgccgtt
    31261 aaaccgcaag ttagtgcgct ggttagcgtg cgggataacg cctgtcagga ttatctcgcg
    31321 caccttgatg gttttaatat cgaatggctg gcacatgatg agcaggcggt agatgcgcgt
    31381 ctggcgcaga tgcaaatccc tgccagatga ttacttcatc tggataaccg gcgaaggtaa
    31441 agtcgttaag aatttaagcc gccgctttga agcggaacag tatgacccac agcgggtacg
    31501 tgcagcggct tactggcacg caaagtaact gatatataaa aagaaaaagg ctgacgattt
    31561 ctcgtcagcc tttgctatat ctggtggccc ctgctggact tgaaccagcg accaagcgat
    31621 tatgagtcgc ctgctctaac cactgagcta aggggccatg gtagcggatt ataaagtaac
    31681 tccgtgtcgc aatccagcca ttaccgcgcg cctgctgttt ttataaacaa tgtattttca
    31741 atccgttata cttttcctgg tgatgttaga aaggagtaaa tatggttgag gatattttgg
    31801 ctccagggtt acgggtcgtg ttttgcggta tcaaccctgg gctttcatcc gccgggactg
    31861 gttttccctt tgctcatccg gcaaatcgct tctggaaggt gatatatcag gccgggttta
    31921 ccgaccgtca gttgaagccg caggaggcac agcatctgct ggattatcgt tgtggcgtca
    31981 ccaaactggt agaccgtcca acggtgcaag ccaatgaagt ttcaaagcag gagctgcacg
    32041 caggtgggcg taagctgatt gaaaaaattg aagattatca gccgcaggcg ttggcgattc
    32101 tgggcaaaca agcatatgaa cagggattca gccagcgcgg tgcacagtgg gggaaacaaa
    32161 cgctcaccat tggttcgacg cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg
    32221 tttcactgga gaaactggtt gaagcgtatc gcgagctgga ccaggcgctg gtagtgcgtg
    32281 ggcgataaaa aacgccaccg a
//
LOCUS       DAJVQH010000034        31722 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJVQH010000034 DAJVQH010000000
VERSION     DAJVQH010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.034"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..303)
                     /gene="ssb1"
                     /locus_tag="OQM87_001654"
     CDS             complement(<1..303)
                     /gene="ssb1"
                     /locus_tag="OQM87_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCS1507823.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTE"
     gene            557..3379
                     /gene="uvrA"
                     /locus_tag="OQM87_001655"
     CDS             557..3379
                     /gene="uvrA"
                     /locus_tag="OQM87_001655"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCS1507824.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3414..3770)
                     /locus_tag="OQM87_001656"
     CDS             complement(3414..3770)
                     /locus_tag="OQM87_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1507825.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(3774..4190)
                     /locus_tag="OQM87_001657"
     CDS             complement(3774..4190)
                     /locus_tag="OQM87_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCS1507826.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4301..5014)
                     /gene="aphA"
                     /locus_tag="OQM87_001658"
     CDS             complement(4301..5014)
                     /gene="aphA"
                     /locus_tag="OQM87_001658"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCS1507827.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5416..5638
                     /locus_tag="OQM87_001659"
                     /pseudo
     CDS             5416..5638
                     /locus_tag="OQM87_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6142..7335)
                     /gene="tyrB"
                     /locus_tag="OQM87_001660"
     CDS             complement(6142..7335)
                     /gene="tyrB"
                     /locus_tag="OQM87_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCS1507828.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7588..8667)
                     /gene="alr"
                     /locus_tag="OQM87_001661"
     CDS             complement(7588..8667)
                     /gene="alr"
                     /locus_tag="OQM87_001661"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCS1507829.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(8720..10135)
                     /gene="dnaB"
                     /locus_tag="OQM87_001662"
     CDS             complement(8720..10135)
                     /gene="dnaB"
                     /locus_tag="OQM87_001662"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1507830.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10218..11201
                     /locus_tag="OQM87_001663"
     CDS             10218..11201
                     /locus_tag="OQM87_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCS1507831.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQPAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11367..11609)
                     /gene="pspG"
                     /locus_tag="OQM87_001664"
     CDS             complement(11367..11609)
                     /gene="pspG"
                     /locus_tag="OQM87_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCS1507832.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(11743..12780)
                     /gene="dusA"
                     /locus_tag="OQM87_001665"
     CDS             complement(11743..12780)
                     /gene="dusA"
                     /locus_tag="OQM87_001665"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCS1507833.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13143..14128)
                     /locus_tag="OQM87_001666"
                     /pseudo
     CDS             complement(13143..14128)
                     /locus_tag="OQM87_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14446..14961
                     /gene="zur"
                     /locus_tag="OQM87_001667"
     CDS             14446..14961
                     /gene="zur"
                     /locus_tag="OQM87_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCS1507834.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15003..15212)
                     /locus_tag="OQM87_001668"
     CDS             complement(15003..15212)
                     /locus_tag="OQM87_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCS1507835.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15328..16653)
                     /gene="dinF"
                     /locus_tag="OQM87_001669"
     CDS             complement(15328..16653)
                     /gene="dinF"
                     /locus_tag="OQM87_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCS1507836.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(16726..17334)
                     /gene="lexA"
                     /locus_tag="OQM87_001670"
     CDS             complement(16726..17334)
                     /gene="lexA"
                     /locus_tag="OQM87_001670"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCS1507837.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17444..17812)
                     /gene="dgkA"
                     /locus_tag="OQM87_001671"
     CDS             complement(17444..17812)
                     /gene="dgkA"
                     /locus_tag="OQM87_001671"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCS1507838.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17983..20406
                     /gene="plsB"
                     /locus_tag="OQM87_001672"
     CDS             17983..20406
                     /gene="plsB"
                     /locus_tag="OQM87_001672"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCS1507839.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20561..21433)
                     /gene="ubiA"
                     /locus_tag="OQM87_001673"
     CDS             complement(20561..21433)
                     /gene="ubiA"
                     /locus_tag="OQM87_001673"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCS1507840.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21446..21943)
                     /gene="ubiC"
                     /locus_tag="OQM87_001674"
     CDS             complement(21446..21943)
                     /gene="ubiC"
                     /locus_tag="OQM87_001674"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCS1507841.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22166..23746)
                     /locus_tag="OQM87_001675"
                     /pseudo
     CDS             complement(22166..23746)
                     /locus_tag="OQM87_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(23975..24895)
                     /gene="malM"
                     /locus_tag="OQM87_001676"
     CDS             complement(23975..24895)
                     /gene="malM"
                     /locus_tag="OQM87_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCS1507842.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLSVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25047..26387)
                     /gene="lamB"
                     /locus_tag="OQM87_001677"
     CDS             complement(25047..26387)
                     /gene="lamB"
                     /locus_tag="OQM87_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCS1507843.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26459..27574)
                     /gene="malK"
                     /locus_tag="OQM87_001678"
     CDS             complement(26459..27574)
                     /gene="malK"
                     /locus_tag="OQM87_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCS1507844.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            27939..29129
                     /gene="malE"
                     /locus_tag="OQM87_001679"
     CDS             27939..29129
                     /gene="malE"
                     /locus_tag="OQM87_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCS1507845.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29283..30827
                     /gene="malF"
                     /locus_tag="OQM87_001680"
     CDS             29283..30827
                     /gene="malF"
                     /locus_tag="OQM87_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCS1507846.1"
                     /translation="MDVIKKKHWWQSDALKWSVPGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITTILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            30842..>31722
                     /gene="malG"
                     /locus_tag="OQM87_001681"
     CDS             30842..>31722
                     /gene="malG"
                     /locus_tag="OQM87_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCS1507847.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGV"
BASE COUNT         8085 a         8121 c         7683 g         7833 t
ORIGIN      
        1 ttctgtggtg tagcgatcct gaccggattg atcggtccat ttacgggtac gcagctgacc
       61 ttcgatataa acctgagaac ctttacgcag atattcgctc gccacttctg ccagtttgcc
      121 gaacagcaca acgcggtgcc attcagtctg ttctttcatc tcgccggtcg ctttatcacg
      181 ccaggattcg gaagtagcca gcgtaatgtt ggcaactgcg ccaccatttg gcatgtagcg
      241 tacttccggg tcctgaccca gattaccaac gagaataacc ttgtttacgc ctctgctggc
      301 catgttcgtg tctcctgaaa aaaatcgttc tgaataagtg taaacgcgcg attgtaccat
      361 taccaatagc gcttttacta tgttgtgacc tcggttccgg gaaacaaacc tggccagaca
      421 ttgttacaca acactccgga taatgcattc caatactgta tattcattca ggtcaatttg
      481 tgtcataatt aaccgtttgt gatcgccggt agcaccatgc caccgggcaa aaaagcgttt
      541 aatccgggaa aggtgaatgg ataagatcga agttcggggc gcccgcaccc ataatctcaa
      601 aaacatcaac ctcgttatcc cccgcgacaa gctcattgtc gtgaccgggc tttcgggttc
      661 tggcaaatcc tcgctcgctt tcgacacctt atatgccgaa gggcagcgcc gttacgttga
      721 atccctttcc gcctacgcgc ggcagtttct gtcactgatg gaaaagccgg acgtcgatca
      781 tattgagggg ctttctcctg ccatctcaat tgagcagaaa tcgacgtctc ataacccgcg
      841 ttctacggtg gggacaatca ccgaaatcca cgactatttg cgtttgttgt tcgcccgcgt
      901 cggcgaaccg cgctgtccgg accacgacgt cccgctggcg gcgcaaaccg tcagccagat
      961 ggtggataac gtgctgtcgc agccggaagg caagcgtctg atgctgctcg cgccaatcat
     1021 taaagagcgc aaaggcgaac acaccaaaac gctggagaac ctggcaagcc agggttacat
     1081 ccgtgctcgt attgatggcg aagtctgcga tctttccgat ccgccgaaac tggaactgca
     1141 aaagaaacat accattgaag tggtggttga tcgcttcaag gtgcgtgacg atcttaccca
     1201 acgtcttgcg gagtcgtttg aaaccgcgct ggaactttcc ggtggtacag cggtagtggc
     1261 ggatatggac gacccgaaag cggaagagct gctgttctcc gccaacttcg cctgcccaat
     1321 ttgcggctac agtatgcgtg aactggagcc gcgactgttt tcgtttaaca acccggcagg
     1381 tgcctgcccg acctgtgacg gccttggcgt acagcaatat ttcgatcctg accgcgtgat
     1441 ccaaaacccc gagctgtcac tggctggcgg tgcgatccgt ggctgggatc gccgcaactt
     1501 ctattacttc cagatgctga aatcgctggc agatcactat aagttcgacg tcgaagcgcc
     1561 gtggggcagc ctgagcgcga acgtgcataa agtggtgttg tacggttctg gcaaagaaaa
     1621 cattgaattc aaatacatga acgatcgtgg cgatacctcc atccgtcgtc atccgttcga
     1681 aggcgtgctg cacaatatgg agcgccgtta taaagagaca gaatccagtg cggtacgtga
     1741 agaattagcc aagtttatca gcaatcgccc atgcgccagc tgcgaaggga cgcgtctgcg
     1801 tcgggaagcg cgccacgttt atgtcgagaa tacgccgctg cctgctatct ccgacatgag
     1861 catcggtcat gcgatggaat tcttcaacaa tctcaaactc gctggtcagc gggcgaagat
     1921 tgcggaaaaa attcttaaag agatcggcga tcgcctgaaa ttcctcgtaa acgtcggcct
     1981 gaattacctg acactttccc gctcggcaga aacactttcc ggcggtgaag cccagcgtat
     2041 ccgtctggcg agccagattg gtgcgggcct ggttggcgtt atgtacgtgc tggacgagcc
     2101 gtctatcggc ctgcaccagc gcgataacga gcgcctgttg ggtacgctta tccatctgcg
     2161 cgatctcggt aataccgtga ttgtggtgga gcatgacgaa gacgcaattc gcgccgctga
     2221 ccatgtgatc gatatcggtc ctggtgcggg tgttcacggc ggtgaagtgg tcgcagaagg
     2281 tccgctggaa gcgattatgg cggtgcctga atcgttgacc gggcagtaca tgagcggtaa
     2341 acgcaagatt gaagtgccga agaaacgcgt tccggcgaat ccagaaaaag tgctgaagct
     2401 gacaggcgca cgcggcaaca acctgaagga cgtgacgctc acgctgccag tcggtctgtt
     2461 tacctgcatc acaggggttt caggttccgg taaatcgacg ctgattaacg acacactgtt
     2521 cccgattgcc caacgccagt tgaatggtgc gaccatcgcc gaaccggcac cgtatcgcga
     2581 tattcaggga ctggagcatt tcgataaagt gatcgatatc gaccaaagcc caattggtcg
     2641 tactccacgt tctaacccgg cgacctatac cggcgtgttt acgcctgtgc gcgaactgtt
     2701 tgcgggcgta ccggaatccc gtgcgcgtgg ttatacgcca ggacgtttca gctttaacgt
     2761 ccgtggcggg cgctgcgaag cctgtcaggg cgacggtgtg atcaaagtgg agatgcactt
     2821 cctgccggat atctacgtgc cgtgcgatca gtgcaaaggt aaacgctata accgtgaaac
     2881 gctggaaatt aagtacaaag gcaaaaccat ccacgaagtg ctggatatga ccatcgaaga
     2941 ggcgcgtgag ttctttgatg cggtgccagc tctggcgcgt aagctgcaaa cgttgatgga
     3001 cgttggtctg acgtacattc gcctggggca gtccgcaacc acactttctg gtggtgaagc
     3061 ccagcgcgtg aagctggcgc gtgagctgtc aaaacgcggc accgggcaga cgctgtatat
     3121 tctcgacgag ccgaccaccg gtctgcactt cgccgatatt cagcaactcc tcgacgtgct
     3181 gcataaactg cgcgatcagg gcaataccat tgtggtgatt gagcacaatc tcgacgtgat
     3241 caaaaccgct gactggattg tcgacctggg accagaaggc ggcagtggcg gcggcgaaat
     3301 cctcgtctcc ggtacgccag aaaccgtcgc ggagtgcgaa gcttcgcata cggcacgctt
     3361 cctcaagccg atgctgtaat cgttaaggcc gctttctgag cggccttttc ctttcagagt
     3421 tgtaccagca atttacgctt ttcttccggt agtaaattca ctgcctgctg ataagacgca
     3481 tccaccagat aatagatttg cgaatccggc agcgaaccgt cgagatacac ggtgctccag
     3541 tgcgctttat tcagatggcg gctaggacgc acatcgctgt gctgctgacg tagcagctcc
     3601 gccagctccg ggctggtttt cagcgaaaca gctgggcgat tttctacttc tttcaccatc
     3661 gcaaacagta catcttccac tttgatctgc gtcgctttcc agtcgttatg cacgctctgt
     3721 tctgcgcctg gttttgccat gcaatattgt agcaactccg aaatggtcat tttttactcc
     3781 ccttgtagtg tcgcgatgat gcgacgcgat ccgccgtgga tgcgatgttc ccccagccag
     3841 atgccttgcc aagtgccggt ctgaatacgc cctttatgta ccggcaatac aagcgatgtt
     3901 cccagcattg aggatttgat atgagaaggc atatcgtctg ctccctcata gtcatgctca
     3961 taatttccgt tgtcgggaac ggtgcggagg aaaaaacact ccatgtcgtg gcgtacggtg
     4021 ggatcgcagt tctcattaag tgtcagagag gcggaggtat gttgcagcaa cagatgcagt
     4081 aagccgatgt taacgcgcgg catatcagcc agctgattca gaatttcatc cgttaccaga
     4141 tgaaacccac gagatttggc gctaagcgtg agcgtctttt gataccacat gtgctgctcc
     4201 ttgataaaac tctcttaatc agtttgcagc aagacagcga aaggataaag gtgtgattaa
     4261 aaaaacagca ttcgggagag catcactctc tcccgctctg tcagtattct gaattgacga
     4321 tcacctcttc accaaacgca cccgcttgtg gcaagggttt gtaggtagag ttggaggcgc
     4381 gcagaatgcg gataccacga gcgccgacat cgcgtgcggc ggtaatatcg ttatcggaat
     4441 cgccataaaa aattcggata tttttatcct gcagccattg cgattttgta ttttgccctg
     4501 gtttatcgcc cgcaaagatt accggattca tgttggtggc aggaatatga aaattatccg
     4561 ccagcgtttt tgaaaccgtt tctgttttcg tcgggctacg accagtcaca aagaagatcg
     4621 cgtcaccgcg gcgtacatgc atatcaatca gctggcgagc gacctctttt ggaatgctga
     4681 attcatccca gccattattc attttttccc agaacacagg atttttcaga taatcttcgc
     4741 tttctggcga gaaggttttt ttgccgcgcc agaaccccgg actggaaaaa agtaccgtgt
     4801 catcgatatc aaaccccacc gccattggcg gacgccctgc gaggctattt tcaatttgtg
     4861 cgaccgaaac ccaatgaatt ggtgcctgtt cagcaagcct ggcaacgtta gtcccagggt
     4921 taagcggtga aggagatgag gccagggcaa cagcggaact gtttagagcg aacaataagc
     4981 aaacggcact gattgcctgt gtgatcttgc gcatattttt ccctaaatag tcagtatgtt
     5041 gaaacttttt attgtgagat ttgttgcaaa aaccatctga ccataacgcc agctgccggg
     5101 gaaaggaagg attatctgcg gtttttgtga tgaaaagcag gatgattata tttaacccag
     5161 taaatacgtt cctgtctttt tttgtataaa aaatgtttat tactcgatgt aaggatacat
     5221 taattttacg tttattatga tgaatcctcg gcaaatgagc gaaatttgat agaacattta
     5281 tctgctgtac gatttaatga acttaatgat acagcattac aaaaacaatc gaagtttata
     5341 aagatgattt ctgattgacc agccccctac cctacaatgg tgattaattt tattactcgc
     5401 gatggagatg atgatatgaa tatatcatat gtgaatagca acaaaacgac atctttacct
     5461 gttgagctag atgcactaaa taataaagat atctcttatg ccaaagattt ctcttatgcc
     5521 aaagatttct ttttatacat agaaacacag ttaaaaattg caaaagattt ttgtagacct
     5581 ggagaagaag tatcaagttc tattgcaagt aaagtttttc acgcatttat tgatttagtt
     5641 aacaaaatca ggggtaagaa agactttatg tatatttgca cgctttgctg ttttgccgag
     5701 gaggttaaag gtgattattc tcattacagg acctttttat ttgatattgg taatcaatat
     5761 aaggttaaac ttatacaaag cggaaaaaaa gagttctctt taacattaga atttaacgat
     5821 actataattg aatctcagaa agtcacaggc aataaggcaa agcatattct tgaggatata
     5881 gaaatattct atcgtaacaa acccgatact tattattaaa tttaataatg gttagatgtg
     5941 cagaaattat tactccacac gaaaaaatag ggaaaggaat tccagataat tgggataaac
     6001 ttaacaataa aagaactgac aacgttttat tatctctcac gtagaacaac ggcatcaaaa
     6061 aaatcatcta caaagaaata gtgaaagcga cgacagttta atttcactgc aggctgggta
     6121 actccagcct gctttcctgc attacatcac cgcagcaaac gcctttgcca cacgttgtac
     6181 atttgccgta tttaacccgg cgacacacat gcgaccgctg gcgatgagat agacaccaaa
     6241 ttcttcacgt agtcggtcaa cctgagcggc acttaaaccg gtataactga acatgccgcg
     6301 ctgattaagc agataatcga aattgcgttc tggcatctct gtgcttaata ccttcaccaa
     6361 ttcctgacgc attgccagaa tgcgagtacg catctcttct acttccgcca gccagctggc
     6421 tttcaatgcc tcgtcattca gcactgcagc caccacctgc gcaccaaaat tcggcgggct
     6481 ggagtagttg cggcgaactg ttgctttcaa ttgccccagt acgcggccag cggcttcggc
     6541 atcttcacac agaacagaaa gtccgccgac gcgctcgccg taaagggaga aaattttcga
     6601 gaacgaattg ctcaccagag cgggtaatcc agcgctggca atggcgcgaa tagcgtaggc
     6661 atcctcttcc ataccggcac taaatccttg ataggcaata tcgaggaatg ggataagctc
     6721 gcgggctttg agaatttcaa tcaccgcatc ccactgatca ttagtgagat cggcacccgt
     6781 tgggttgtgg caacatggat gcagcaacac aatactgcgg gcaggtaatg ttttcagcgt
     6841 cgccaacagg tcattaaagc gcacgctgtt agtcacttcg tcataccagg ggtaagtact
     6901 cacttcgaat ccagccccgg cgaatattgc tacgtggttt tcccaagtag gatcgctgac
     6961 ccagacgcct gattccggga agtagcgttt caggaaatcc gcgcccactt tcaatgcccc
     7021 tgagccgcca agggtttgaa tggttgctac gcgctgttgt tgcagtaccg gatggtccgc
     7081 accaaacagc agcggcgcaa tggcatggcg atagctgtta agcccttcca tcggtaaata
     7141 aagcgaagcg ccatggggct gcgcattcag gcgcgcttcc gcctccgcca cggctttcaa
     7201 ttgtggaata attccgtctt cgttgtagta cagaccgata cttaaattca ctttgtcgct
     7261 gcgagggtct tctttaaaac gctccataag cgtaagaatc gggtcgccag cgtaggcgtc
     7321 aactttttga aacacgcgat ggttctccag gtttacgggc aggtggttaa aacacaataa
     7381 accggaagaa ggcgaagatc gagtggatgt tcaggagcga acggcaatta gcaacagagt
     7441 gagactaatg ataaacgtac atccgccaga ggcacgacct ttataagagc atgaaagaac
     7501 atcaacttat tgaattttta ggattttatt ggccggataa ggcattcacg ccgcatccgg
     7561 cacagacaat caaatattac agaacgatta atccacgtat ttcatcgcga cccttgaagt
     7621 caggcgcgta ataagttcgt aagcgcttac tttcgtcatt tcagcgatac gttctacggg
     7681 caaaccttcg ccccataaaa tgaccggatc cccggctttg tcctgcgcct gtggacctaa
     7741 gtctacgcag atcatatcca tcgccacgcg cccgacaatc ggtacttcgc gaccgttcac
     7801 cagcactggc gtaccggacg gcgcggcgcg cggataacca tcgccatagc ccatcgcgac
     7861 tacgccaaga cgggtatcac gttcgcttac ccaggttcca ccataaccaa caggctctcc
     7921 ggctttatgc tcacgcacgg caatcaggct ggaggttagt gacatcactg gctgacagcc
     7981 aaaatcggca ccggtggagc gatcttccag cggcgagacg ccataaagaa tgatgcccgg
     8041 gcgcacccag tcaaaatgcg actgtggcca cagcagaatg ccacccgacg cggcaatgga
     8101 acgttgacca ggtttgcctt cgcaaaaggt attaaagata gcgagttgtt tctcggttgc
     8161 gccacatttt ggttcatccg cgcgcgcaaa atggctgacg atattcaccg gctgacgaac
     8221 gtttttgcac tgggtcaggc gatgataaaa cgcctcagcc tgttccggcc ttacgcccag
     8281 acggtgcata ccggtatcga gtttcatcca gacggtaacc ggctcgtcca agctagcctc
     8341 ttccagcgca gccagctgtt cttcgttatg cacggcggta tgaaaatgtt gcgcagaaat
     8401 cgtcggcaga tctctggcat caaaaaagcc ttcgagtaac agtacaggtt tggtgattcc
     8461 ccccgcacgc agtcgcagag cttcttcgag acgggctacg ccaaaggcgt cagcatcggg
     8521 gagcgttcgc gcggtctcaa gaagaccgtg accataagcg ttcgctttca ccaccgcaac
     8581 cattttactg gcaggtgcca gttcacgaag acgttgcagg ttgtgtcgca gagcgcggcg
     8641 gttaatcaca acagttgccg cttgcatttg tgttccttga taattgtttg ctttaattac
     8701 ctaattcata aaataattat tattcgtcgt cgtactgcgg ccccgcatag ttgtcgaagc
     8761 gcgaccattg accgttaaag gtcaggcgta ccgtcccgat tgggccgtta cgttgtttac
     8821 cgataataat ttccgcgatg ccttttaaat cactgttttc gtgatacacc tcatcacgat
     8881 agataaacat gatcaagtcc gcatcctgct cgatagagcc agattcacgc aggtcggagt
     8941 tgaccggacg tttgtcggca cgttgttcca gagaacggtt caactgggac agcgccacca
     9001 ccggcacgtt cagttctttc gccagtgctt tcagcgagcg ggagatttct gcaatttcca
     9061 gcgtacggtt atcggaaagc gccggtacgc gcatcagttg caggtagtcg atcatgataa
     9121 gcccgatgcc gccgtgttca cgggcaatac ggcgtgcgcg ggaacgcact tccgttggcg
     9181 tcaggccgga agagtcatcg atatagatat tgcgtttttc gagcaaaata cccatggtgc
     9241 cggaaatgcg cgcccagtct tcatcatcga gctgcccggt acggatttta gtctggtcaa
     9301 cgcgcgacag cgacgccaga gaacgcatca tgatctgttc tgatggcatc tccagcgaga
     9361 agataagtac tggtttatcc tgcaacattg ccgcgttttc gacgaggttc atcgcaaatg
     9421 ttgttttacc catcgacgga cgcgcggcga cgatgatcaa atccgacggc tgcaagccag
     9481 cggttttttt gttgagatcg tcataaccgg tgtttacccc ggtaacgcca tcgtgtggct
     9541 gctgaaacaa ctgctcaata cgcgccacgg ttgcgtcgag cacatcggcg atgttcttcg
     9601 gcccttcgtc tttgtttgca cgactttcgg caattttaaa gacgcgggat tcagcaaggt
     9661 ccagcagatc ttcgctggta cgcccctgcg gatcaaaacc ggcttcagca atctcattcg
     9721 caaccgagat catctcacgg acaacggcac gttcacgcac gatgtccgca taagcactga
     9781 tgttcgccgc acttggcgta ttttttgaca gctctgccag ataagcaaaa ccaccgacgc
     9841 tatcgagttg cccctggcgt tccaacgatt ccgcaagggt aatcagatcg ataggactac
     9901 cgctttcctg caaacgcgcc atttcagtaa agatatgacg gtgtgggcgg gtgtaaaaat
     9961 cgtctgctac cacacgctcg gctacatcat cccagcgttc gttatccagc attaaaccgc
    10021 ccaacaccga ctgctccgct tcgatcgagt gcggaggcac tttcagcccg gcaacttgtg
    10081 gatcgcgttc gcggggttca gcctgctgtt tgttgaaggg tttatttcct gccatagtga
    10141 atggagttac cgaaataaag aatgggtcga aactttacca tatgaagcag accctgacga
    10201 tacgttctgg aggacacatg gcaacacgaa ttgaatttca caagcacggt ggcccggaag
    10261 tacttcaagc cgtagagttc actcctgccg atccggcgga gaatgaaatc caggtcgaaa
    10321 ataaagccat cggcatcaat tttatcgaca cgtatatccg cagcggcctt tacccgccgc
    10381 catcgctacc cagcggatta ggtaccgaag cggcaggcat cgtgagtaaa gtcggcagtg
    10441 gtgtaaagca tattaaggct ggcgatcatg tagtctatgc gcagtcggcg ttaggcgctt
    10501 acagctctgt gcataacatt aatgcggata aagcggcgat tctgcctgcg gcaatttctt
    10561 ttgagcaacc tgcggcatcc ttcctgaaag gcttaacggt ttattatctg ctgcgcaaaa
    10621 cctatgaaat taaacccgat gaacagttcc tgttccacgc agcggctggc ggcgttggct
    10681 taattgcctg ccagtgggca aaagccctgg gcgcgaaact catcggcacc gtaggaaccg
    10741 cgcaaaaagc gcagagcgcg ctaaaagccg gcgcgtggca ggttattaac tatcgtgaag
    10801 agaatctggt cgagcgattg aaagagatca ccggcggtaa gaaagtgcgc gtagtgtacg
    10861 attccgtggg cagagacacc tgggaacggt cgctggattg cctgcaacgc cgcggcttaa
    10921 tggtcagttt tggcaactca tcaggtgcgg ttaccggtgt gaacttaggc attctcaatc
    10981 aaaaaggctc gttgtatgtg acacgccctt ccctgcaagg ctatatcacc acgcgggagg
    11041 aattaaccga ggccagtaat gaactgttct ctttgattgc cagcggtgtg attaaggtcg
    11101 atgtcgccga gcagcagaaa tatccgctga aggatgcgca gcgtgcgcat gagattctgg
    11161 aaagccgggc gacgcaaggt tccagcctgc tgattccata aaagaaatag ggcttccacc
    11221 tgggaagccc tttcttttta tagttcggct gtatgtaggg tacagcacga tgaatctgtt
    11281 agaggcgcaa tagtgacaga tttgattatc aattcctatt ttgttctaag gataaaacct
    11341 taggttgtga tcatccgcac aatcccttag taacgccagc ggtcataacg ctgatatttc
    11401 ggcatttttg gtgctttaat cgccttaata acccacacca ccgcaatcgc cagtagtaac
    11461 cacggcagca acttaatcat caatgccagc ataccgccga ggaacataat ggccgtcgcc
    11521 acaaccagcg cggcgataat gcccagcaac gaaacgccgg tgaccatcag catgacaaaa
    11581 aagccaatca caaaaagtag ttccagcatg atgctctccc aaatatgaaa tctcttgctg
    11641 gcattacaag aatcatgcca aaaataatct attgatttaa cagcaaaacg ccccgcgacg
    11701 gtgcgcaggg cgtggtgaat ttgactactt tttggtgaaa agttaacgct tatccgccac
    11761 cagtttgagc gcgtgttcca gcacgttaat gtcagcaccc gctttatggg cattttcact
    11821 taaataacgc cgccactgcc gcgcgccagg aataccctgg aacaaaccca gcatatgccg
    11881 gataatatgg ccgagatacg tcccctggct gagttcatgc tcaatgtacg gatacatggc
    11941 acgcactact gccaccggat cggcatcggt atccgaggaa ccaaagatct cccggtctac
    12001 cgccgccaga atacccggat tctgatacgc ctcgcgcccg accatcacgc catccatatg
    12061 ttgcaaatgc gctttagctt cttccagcga cttgatacca ccgttaatcg acattgtcag
    12121 atgcgggaag tcacgcttca gttgatacac acgcggataa tcgagcggcg ggatttcacg
    12181 gttttctttc gggcttaacc ccgaaagcca ggctttacgt gcgtggatga tgaacatctc
    12241 acactcgcct ttgccggaaa cggtattgat gaaatcgcag agaaattcat agctgtcctg
    12301 gtcgtcgatg ccaatacgcg ttttcaccgt caccggaatc gacaccacat cgcgcatcgc
    12361 tttcacgcag tcggcaacca gctgcgcatt acccatcaga cacgcaccaa acatgccgtt
    12421 ctgcacccgg tcagacgggc agccgacatt caggttgatc tcatcatatc cgcgcgcttc
    12481 tgccagcttt gcacactgcg ccagcgccgc cggatcgcta ccgcccagtt gcaacgctac
    12541 cggatgttct tcttcactgt acgccagata atcaccttta ccgtgaataa tcgcccctgt
    12601 ggtcaccatt tcggtataaa gcaacgtatt gcgggaaagc agacgcaaga aatagcggca
    12661 atgtctgtcc gtccagtcga gcattggtgc aacactaaac cgaccactcc agtgaacgtc
    12721 agttttttca ggcattgcgc tggtttggtt gattttttgc atttcagaat taccgtgcat
    12781 tttcaaatgt agagattatt ttatcgatat atcatggggt tatgttattc agcttcactg
    12841 ttcaggaggc tcaatagcgg ggtactatac cataacaaca ggaagcgcct gtctcattgc
    12901 aaaagaaaat tgagatcctc tcaaggcatg aagctctcac gaagtgatgg aaataatctt
    12961 attagccgtt aactttgtta aggccaatga taaacaatcc aggttcgacg ataaataaaa
    13021 aatcacacat taaactctgg tgatatatct ccctgctaat agcattgata gagaaaaaag
    13081 aacccaataa atattgggtc cttttatata atgccttcca tactattgaa gaacttcaca
    13141 tattatttct ccgatttaac cccgaacaag tcataaatta atttagaagt gtctgtaagt
    13201 attttaatct cttcctttga ggttgggtca aacgacttcg cgaagctaat taattgtggt
    13261 gcagcatctc gcattttgct taaaatatca ggatcgagcg ttccttcatt caccagatgt
    13321 gacatcttat ccagatagcc atcaaaattc atttctcccc catctgccag acgcttcatc
    13381 tctcccaggt acttcttcat atcacgttga tataattttt caaactgagc tttcaggtag
    13441 ctctcattat tttcattaat atacactgtt tctgataaat ctgcgtaggc actagaatat
    13501 gaaagtgaaa ttaacagcga agcaaataat tttaagctgt tgtcatcatg ttcctggcag
    13561 gcattaacaa acgttaaaaa accagaacct attgcttgaa aatgtatatt cgctaaaggt
    13621 tcattatcct tagattcctc ataaaatagc tgaaccgtgg agggaaggcc attcttagtt
    13681 tctacatcaa aagtacattg ttcaatgggc attgcatctt tattatcccc aggattacat
    13741 atactattaa tcgcgaaatg aaaaatagcc cggtcaacaa ctgatgcaat aaagtcacga
    13801 tcgtttaaat cctcatcagt gcaatcgtca gtatacttat tgacccacat tttgagatca
    13861 tttatttgct caattaaaaa cacatttttt tacgtgcatg ccataaatta tgcgtgattc
    13921 gttgaataaa caaatctatt tttgcaataa ttatatcccg atgacgcagc gctgcataat
    13981 caatagattt accatatata ttatttaatt tacctttgat tgaacctcta ttatccaatt
    14041 tatctttggt tgcatcttta ttttcgaatt gatttgaatt gagtgtttta ttaaagtatc
    14101 cagttgcacc gggtattatc ttcaacatga ttcatctacc ttttttgtcg taccgtgtcc
    14161 tccttataat tcttttgcca tctggtcact atcaaaaatc gctcataatt tctgaactac
    14221 aggtatgcgt ttaaacccag ttaaataaat agcgagttaa cttgaagcaa tttatttaaa
    14281 aatgaattat taattattat cgtgttgata atggtacgac aaggcaacac aacaccttac
    14341 ctacaaaaca gaaaatcaac attacggcaa caataagggt tctcggtgtt tttcctggaa
    14401 catggtaaag taaggacatt cttaaccccc actttgaggt gcccgatgga aaagaccaca
    14461 acgcaggagt tattagcgca ggctgaaaaa atctgcgcgc agcgtaatgt gcgcctgacc
    14521 ccacagcgcc tggaagtgtt gcgcctgatg agtctgcagg atggcgctat cagcgcttat
    14581 gatctgcttg atttactgcg cgaagctgaa ccgcaagcca agccgccaac ggtttatcgc
    14641 gcgctggatt ttctgcttga gcaaggtttt gtgcataagg tggaatccac caacagttat
    14701 gtgctctgtc atctgttcga ccagcccacg catacgtcag ccatgtttat ttgcgatcgc
    14761 tgcggcgcgg tgaaagaaga gtgtgcagaa ggcgtggaag acattatgca tacgctggcg
    14821 gcaaaaatgg ggtttgccct gcggcataat gtgattgaag cacatgggct ctgtgcggca
    14881 tgcgtggaag tggaagcgtg tcgtcatcct gaacagtgcc agcatgatca ctctgtgcag
    14941 gtgaaaaaga aaccgcgtta agagggcgta catccttgta cacgtcgggc aggagggatt
    15001 aattaccagc gatattcatt gcgggtttcc caatccacga cctctttttc tgcctgatct
    15061 ttctgataac cataacgttc ctggatttta ccgaccagtt gatcacgttt accttcaatg
    15121 atcgtcatat catcatcggt cagtttgccc cattgctctt tcactttacc tttaaactgt
    15181 ttccagttac cgccggcttc atctttattc ataatcaaga cctcatcgtt aggttgtgaa
    15241 tgagagtacg ttcacttttc ttctgaacgt gagattaagt gtagtcagca atttggctta
    15301 ggataattta ttcagaattt ttaaccgtca cgttgcggca aaccaggtac cgttgcgcca
    15361 gtgacgccgc cagatagccg ccagagaaag cccgcgcaac gccagaaaga cggttaatgc
    15421 cagccacaaa ccatgattac ccagccacgg cagcgtaagg agcgtcagcg caaaacctgc
    15481 ggcggccacc gccatactgt tacgcatttc ggcggcacgc gttgcgccta taaacatgcc
    15541 gtccagcaga taacaccaga cgccaaccaa cggcaaaatc acctgccaga taagatagcg
    15601 gtcagccagc tgctgaatct gggttaacga cgtcagcaac gcaatgatgt gttcccctgc
    15661 cagcaaataa accaccgaaa acagtaacgc tacaatcccc gactggcggc acgctgcccg
    15721 ccatacatcc agcaactggc taccgtcgcg cgcaccgtat gcctgaccgg agtgcgcttc
    15781 aaccgcgtag gcaaaaccat caagcgcata ggcggtaaag gtgagtagcg tcatcagaac
    15841 cgcgttaaca gcgataatgt cactccccag tcgcgcacca agtacggtga tcgcgccgaa
    15901 acagagttgc aacaacagcg aacgcagcat gatatcgcgg ttaagcgcca gcaagcgacg
    15961 gaagtttcct cgccaggcag ttttcagcat ttcgccggaa attccgcgta gtttgaggat
    16021 tttacgcacc attagcagac caatcagcaa tgttgcatat tccgcaataa ccgtcgccag
    16081 cgccgcgccc tgcacgttca tatgcagccc catcaccagc cagacatcca gcacaatgtt
    16141 gaggatattg ccgaccacta acaaaattac tggcgcacgg gcatattgca cgccgagtaa
    16201 ccaaccgagt aataccagat tcgccagcga cgccggagcg cttaaccagc ggatttcaag
    16261 aaagcgccgc gcctgttcca ggaccgcttc actaccgcca acaatatgca gcgccagatc
    16321 gataatcggc gtacgcaaca gcgcaattaa cgccccggcc cccaacgcca acagcaacgg
    16381 ttgcaccagc gcacgggcta atgcctgagg atttttggca ccataagcct gcgcagtcag
    16441 cccggtggtg ctcatgcgta aaaacagcaa cagcataaag agaaagctgg tcgccgttgc
    16501 accaaccgcc acgccgccca aataaaccgg actatcaaga tgaccaatta ccgccgtatc
    16561 gaccagtccc agcaacggaa cggtgatatt ggagaaaatc atgggtaagg cgagatgcca
    16621 gagtgcttta tcagatgaag tgaggaatgc catgcagaca agcctgatga agagagatga
    16681 aaaacaaacc gcgacgccag gcggcatcgc ggtctcagag atatgttaca gccagtcgcc
    16741 gttgcgaata accccaaccg ccagcccttc aatggtgaag ctctgctgac gaaggtcaac
    16801 gacaattggt ttaaactcgc tattttctgg caacagttcg actttattgc cctgtttttt
    16861 caggcgctta acggtaactt cgtcatcaat acgtgcgaca acgacctgac cgttacgtac
    16921 atcctgagtt ttatgcactg ccagcaagtc gccatccata atgccgatat ctttcatcga
    16981 catcccgctg acgcgcagca ggaaatcagc attcggcttg aacaaggaag gatcgacctg
    17041 ataatgacct tcaatatgct gttgcgccag aagcggttca ccggcagcca cacgacctac
    17101 cagcggcaac ccttcttcct cttcctgcaa cagacgaatc ccgcgtgatg cgccggaaac
    17161 aatttcaata acgcctttgc gtgccagcgc cttcagatgt tcttcagccg cgtttgggga
    17221 acggaacccc aaacgctgcg cgatttccgc acgcgtcggc ggcatacctg tctggctgat
    17281 gtgatcacgg atgagatcaa acacctcttg ttgcctggcc gttaacgctt tcattccgcc
    17341 ccctgggtgt atatacagtt atactgtgag tatatacagc aaaaggcgat tttggaacca
    17401 taaactgcac aataaaccag agatttatcg aaatctggaa gggttatcca aaatgcgacc
    17461 ataacaggat gcaccaggta atcacggcga cgataatggc aatcagcacc gcggcggatc
    17521 ccatatcttt tgcgcgtccg gaaagctcat ggtattcaga gccaattcgg tcaaccactg
    17581 cttcgatggc gctattgagg atttccacaa tcatcaccag catcacggag ctgataagca
    17641 gcacgcgggt aattgcgtcc acatccagcc agcaggcgat gaccaccgcc aacaataccg
    17701 ctacgccttc ctgacggaat gccgcttcgt tgatccatgc agcgcgtaaa cctttccagg
    17761 aatagccagc agctttgata attcgggtga atccagtggt attattggcc attgaaagaa
    17821 cctttttaca ttatgagcgt caatatcagt gtaccgttaa atccgatgat tcgggtttgc
    17881 gtaacgccgc gcgaaacatg agcggatacc acagaatttc ccatgacttt ctgctatcct
    17941 tgccgcgcat ttgcattatt aaccagaggc tttacatcgt ttatgtccgg ctggccacga
    18001 atttactaca aattactgaa tttaccatta agcatcctgg taaaaagcaa gtctattccg
    18061 gcagatcctg ccccggaact ggggctggat acctctcgtc caattatgta cgttttaccg
    18121 tacaactcga aagcagattt gctgacgttg cgcgcccagt gtctggcaca tgatttgcct
    18181 gacccgttag agccgctgga aatcgacggc acgctactgc cgcgctatgt gttcattcac
    18241 ggcgggccgc gtgtattcac ctattacacg ccgaaagaag agtctattaa gcttttccac
    18301 gactatctcg atttgcaccg tagcaaccca aatctggatg tgcagatggt gccagtatcg
    18361 gtgatgtttg gtcgcgcgcc ggggcgtgaa aaaggcgaag tgaacccgcc gctgcgtatg
    18421 cttaacggcg tacagaaatt tttcgctgta ctgtggctcg gtcgcgacag ttttgtgcgt
    18481 ttctcgccgt cagtgtcgct gcgccgtatg gcggatgaac acggcacgga taaaactatc
    18541 gctcagaaac tggcgcgcgt ggcgcgtatg cactttgccc gtcaacgtct ggctgccgta
    18601 ggcccacgtc tgcctgctcg tcaggatctg tttaataagc tgctcgcctc ccgcgccatt
    18661 gccaaagcgg tagaagatga agcgcgcagc aaaaaaatct cccatgaaaa agcgcagcag
    18721 aacgcgattg cgctgatgga agagattgcg gcgaatttct cttacgagat gatccgcctg
    18781 actgaccgca ttctgggctt cacctggaac cgattttatc agggcatcaa cgtccataac
    18841 gccgagcgcg ttcgccagct ggcccacgac ggtcatgagc tggtgtatgt gccttgccac
    18901 cgcagtcaca tggactacct gctactttct tacgtgctgt accaccaggg gctggtgccg
    18961 ccgcatatcg ccgccgggat caacctgaac ttctggccgg ccgggccgat tttccgccgt
    19021 ctgggggcgt tctttattcg ccgtactttt aaaggcaata aactttattc caccgttttc
    19081 cgtgagtatc tcggcgaact gttcagccgt ggttattccg tcgagtactt cgtggaaggc
    19141 ggtcgttccc gcacggggcg tttgctggat ccgaaaaccg gtacgctgtc gatgaccatt
    19201 caggcgatgc tgcgtggcgg cacgcgtccg attacgctga ttccgatcta tatcggttat
    19261 gagcacgtca tggaagtggg tacttacgcc aaagaactgc gcggcgcgac gaaagagaaa
    19321 gagagcctgc cgcagatgct gcgcggttta agcaagctgc gtaatctcgg tcagggttac
    19381 gtcaacttcg gtgaaccaat gccgttgatg acctacctta accagcacgt accagactgg
    19441 cgtgaatcta tcgatcccat cgaagcggtg cgtccggcct ggttaacgcc gacggtcaat
    19501 aatattgctg ccgatctgat ggtacgcatt aacaacgcag gcgcggcaaa cgccatgaac
    19561 ctgtgctgta ctgcgctact ggcatcacgt cagcgctcac tcacccgcga gcagttaacc
    19621 gagcaactca actgctacct ggatctgatg cgcaacgtac cttactccac ggactctacc
    19681 gttccttcag ccagcgccag cgagcttatc gatcacgcgc tgcaaatgaa caagtttgaa
    19741 gtcgagaaag acactatcgg cgacatcatc attctgccac gcgagcaagc ggtgctgatg
    19801 acctactatc gcaacaacat tgcgcatatg ttggtgctgc cttcgctgat ggcggcaatt
    19861 gtcacccagc atcgccacat ctcccgcgac gtattgatgg agcacgtcaa tgtgctttac
    19921 ccaatgctga aagcggagct gttcctgcgc tgggatcgcg acgagttgcc ggacgttatt
    19981 gatgcgctgg caaatgagat gcaacgtcag gggctgatta ccctgcaaga tgatgagttg
    20041 catatcaacc cggcgcattc tcgcacccta cagctgctgg ccgcaggcgc gcgcgaaacg
    20101 ctgcaacgtt atgccatcac cttctggttg ttgagtgcca acccgtcgat caaccgcggt
    20161 acgctggaga aagagagccg caccgttgcg caacgtctct ccgtgctgca cggcatcaac
    20221 gcgccggagt tcttcgacaa ggcggtgttc agttctctgg tgctgacgct gcgtgatgaa
    20281 gggtatatca gcgatagcgg cgatgccgaa ccagcagaaa cgatgaaggt ttatcagttg
    20341 ctggcggagt tgattacatc agacgtgcgt ttgacgattg agagtgcgac gcagggcgaa
    20401 gggtaatcag agagaattgc cggatgcggc gaaaacgcct tatccggcct accatgacct
    20461 gcaaattcaa taaattgcga ttcaccgggt aggcctgaga agcgcagcgc atcaggcaac
    20521 ccagaagaaa gccggatgat catccggctt ttttacatca tcagaaatgc cagtaactca
    20581 ttgccagtcc taaaaacagt accagaccaa catagttatt attcataaat gctttaaagc
    20641 aggcttcacg ctcgcggttg gcaatcagtt tttgttgata aacaaacagc gcgccagcca
    20701 ccacaattga ccagtaatat ccccagccta agccatttaa ctcaccgatg atcgccatca
    20761 gtgccagtac gccaatctgc aaaataccaa taatcaattt atcgtattgg ccgaacagga
    20821 ttgccgtgga tttaatgcca atcttcacat catcatcgcg gtcaaccatc gcatactgcg
    20881 tgtcgtaagc caccgcccag agaatattgg cgaggaacat taaccagcaa ctcaatggca
    20941 ccgactcact cacagcggca aaagccattg gaatcgacca gccaaacgcc gcgcccagca
    21001 ccacttgcgg gagatgggta taccgcttca taaacgggta cacccacgcc aatgccagag
    21061 cggcaatcga caacagaatg gtcatcgtat tcagcgtcag aaccagtaaa aacgaaatca
    21121 gtaccagcac tacaaacagc gcgcgcgcct ctttctctgt taccgcgccg ctgggaagtg
    21181 gtcggttcgc cgtgcgctta acatgaccat caaacttgcg atcagcataa tcattcacca
    21241 cgcaaccagc agcgcgcatc agccagacac ccgcgacaaa caccgccagg atccagagct
    21301 gaggaacgcc cggtgtcgcc acccacaacg cccataatgt tggccagagc agcaataacg
    21361 cgccaattgg cttatccgta cgcattaagc gatgaaacgc cagcagctta ttctgcgtca
    21421 gactccactc catatttttt tcctcttagt acaacggtga cgccggtaaa aacagttctg
    21481 ttagcaacag cggtttaccg cttaatcgca ggcgggaacg tcgcccccac agtccggcat
    21541 cacggcctat ctcgataaaa tcccgggtta gtgtcgatga tgtgaacaga tagcgtccta
    21601 acggcgtttt acccaatttt tgtaacgcta gttccggccc gcttaatgtt gatacaggaa
    21661 cgacggtacg accggcaagc cacggttcac catcggcaca taacaaaatt tcacgtaacc
    21721 agtaacgaga ctctttcggc agtagcggca gttcttcggg gatttcattc tgctcgacaa
    21781 acccctcgcg gatcatcgtc acactcaccg tttttccctg ctgttcaaaa cgttttgtca
    21841 tggaatcctc cagcaacagc cagtcgaaca gttgcggatc cagggcaggg atctctttaa
    21901 aatagcgcag cgcacgcagt tgcgttaacg cggggtgtga catgccgaac tctccgttac
    21961 ataacgtaaa ggcattgtat cgcagaaaag tgtttacggg agatgtaaag caaattaagt
    22021 gatctaatgc gcaacaaaaa cgcaacaatc gcatgaagtg gtaaaacgct gttgaacctt
    22081 acgctttgcc gatgcccgtg ctggcgaggg caaccatagg ggcatcaccg cccatgatgc
    22141 taggaacggt gatgttttcc tttacttaaa tacctttatt ctttatacga gtagctcgcc
    22201 aactcgcagt aaaaattggg aaaagcgcat atttaccatg agatttggag ctgaaaagtg
    22261 tttcctgagt aagattaata ggcaacatct tatgtctgga tttatagata cacgtggaat
    22321 tccaattggt atcttttgta ggattcaaca tatcatgtaa gcgctgggag cttaccacca
    22381 taacaggccc atcatcgaca gaggagaaca ggataaatat attatcgttt cgtgttaatt
    22441 tattagtgtt aaaggttctt aacttttctt cagtttctat aaatataggt gaaacatcat
    22501 cactacgaat ccactcttta taaagatacc aggcatttgc tttgatcata tcatccgttg
    22561 atcgtaaggc ctgatcgata agttggataa aaaaattgtt atttttatca accagccctt
    22621 tattcatatt aaatctgtta ataaaatgga tcatctgttg ggtatcgaaa ttcaccttac
    22681 tgaatcgatg ttgacgcata aaattatcga acttgtcatc ttccccagaa tgtatataac
    22741 tacgtataat tggatctttt atataaggac aatggctaaa tgttttcttc atattctcaa
    22801 taaagttttt attaacaata ttgagaattt ttaattgcat caccagtttt actgccagaa
    22861 cggatttcaa tatcattgca ttatctggca atgtatcaat catttttata atgttattga
    22921 tataaccatc gagatcttca ttcccaaata tatctgaata atcaatagtt aattccaatt
    22981 gttctgtaat gcaatcttaa aaattccctg tcgacattcg cacatgatca aacataacag
    23041 ctgtcaaatt cgatttaata aatgatattt ctttggcaga cagaaactct gctctggtga
    23101 agtgcgaagc gtaaagataa caattatcga agtttgaatt tctaatctga cattcgcgaa
    23161 aattactatg accaagttga ctctgcgaga agtcaactgt atcaagaata ttttggctaa
    23221 aatcaacaca ttcgccactc gttttgagaa aaatcacatt gtgtaaacgt cgacatttaa
    23281 atatgacatt atgaactgtt gccttagtaa aattgcaatt ctcaatttct ttacattcga
    23341 aagttacatg ttgtagattt gatgataaaa aagacacctt agaaataacg tcacctttaa
    23401 aatgacaatt tgttaaatca aaatcagaca ggtcgatata cccctgcgca tctttatcca
    23461 tcagaaaatt acaagcgcat tctatttttt catttctgta gaattttaca cttatagtat
    23521 cattactaat tgagacttca cgtaacatat ttattgcatg atttttttcg ttccgttgtt
    23581 ttatatttaa aaatatatca ctgatataaa taaagttatc tctgtttaaa cctgtcaccc
    23641 cattgaaatt acgtagtaaa tcaagatcta ctatattatt tccttgcacc acatcatgtt
    23701 tcatcaaaga gttattgccg ccagaatttg tgatatgacc tatcataaaa cctctgtaat
    23761 acagaaataa aataacctgg aaaagtagcc tgagcgtcca gacactaccg gcgaaaggaa
    23821 aagaatctct gataatgcat tgaaataatg gacattctta gtgaagaaga agtgaagtac
    23881 tataaaaaat cgctcatatc ccaggaagga agcataaaaa aaggtgcgcc aggagacgca
    23941 ccagttgttg caaaatcagc actgtggggc gtaattaccc cttgcctttt acactgctga
    24001 taaaggtgga acgggcagat gtcgatccca ggcgttcagc ttcatcaagc agttttaacg
    24061 ccttatcaac atcacctttc gcgacagcgt ttttgatcgc ggtattaaaa taactttccg
    24121 tgtcgttgag catcggctcg cttttcttca ccggcaccgg agcaggtgca gccacagctg
    24181 gtgccgccgt gttgcctacc gtaaccggag ctggagcgga agaaccaaat agtggtccta
    24241 ccaacacgct ggagctggag ttcgttttga ctttcagttt cagtaagcca tcgctggtat
    24301 gacgagcaac cggatcggga atatccggga tcgagttacc gacgcctttg gcataggctt
    24361 tagccgggtc gagcagttgg gtcgtctgct ggagatcttt ttccatggta aagaccagaa
    24421 cataaagttt ttgctgcccc aacgccggtg tcaggcgcat aacgccttcc agccgatctg
    24481 cactcatcac gcctagttcc tggtaggtga aataactgct ggggaagaag gctgatgggg
    24541 tcatgttctg atcaataatc agcacgttcg gcgcaaaaac gctggtttgt ttgttcactt
    24601 cgctggtcag cgtcagggtc agttcgccaa tgtttgccgg gacgctgtac gcagcaaccg
    24661 gaccactgat gccgggaacg ctcagttgtt ggccgccggt cgccagttgg gtggtctggg
    24721 ttttagattg atcgaccggt gtccaggtga gttgttgcag cgcggcagat ggaatggctg
    24781 gcgcgtcgct ggtgttttgc ggtacgtagt taacatcggc aaggctaatt ccaggcgcgc
    24841 ttgccagtaa ccctgctgat aaacagaggg cgatgagact tttattcatt ttcattgttt
    24901 tcacctcaaa atctggagct cagcggtagc caggcaatag cgcgctaaac ccgataatca
    24961 gaggggcttt cgcccctttc agataatgac aacctgtttt tatgccggat gcggcgtaaa
    25021 cgccttatcc ggcccaggtt ttgctattac caccagattt ccatctgggc accgaaggtc
    25081 cactcgtcgc tgtcgccacg accgaagctg ccgccgttga aatcagcagg aacggctttg
    25141 ccgtagttcg ggttaacctt gctatcgccg ttgtagtcgt aaccccattt ctcatcccac
    25201 ttggcgtagg ttgcgaagac acgaatagcc gggcgtgacc agatgctgtc gccagcctgc
    25261 cattgttgtg cgagggtaat tttgtactga ttgttcttgt cgccggtgcg ctgggattcg
    25321 acgttgtcgt agccgatttc catcacggtg ctcatgattg gcgtccactt gtacatcggg
    25381 cgaataccaa cggtccacca cttggtgccg ttgtcgttat cccagttgat atcctggtac
    25441 atacccacgt acatcatgtc ccagttgtcg cccatggaga tcgcaccgtg gtcgaggata
    25501 cgcagcatgt gaccgttgtt gttgatattg taggcaaatt tttcgttatc aaacgcgacg
    25561 ccagaaccct gcgacagacc tttaccctgc gaggtcatcg agtcagtagc gtactgaaca
    25621 acaaacttgt taaagccctt caggacactc tgagtatgtt cagcagtgaa taaccagccg
    25681 tctttcgatg cgccatcaac cagacgatag ttatcacgca ggttggcacg accgtagtcg
    25741 acacccagtt ctaatgtgcc gcccgggttg atttccatct gcgctaaacg cacatcgaaa
    25801 acgtcgttcg cggtttcgtt ggtatagtca taaatattgt tgctggcaaa agaggaagaa
    25861 ccaccagctt cagaggagcg ggttgctgcc agagagagtt taccgaagcc aacatcgatg
    25921 ttttccagac cggcaccagg accagaaata tcccagtagt agaagtcgat catatgaacg
    25981 tcatgacgtt ggtagaagcg cttacctgcc cagatggtgg aacctggcag ccattcgatc
    26041 aggttttttc cctgcacgtt tgcttcacgg aaggccggat cggtagcttc ccagtcattc
    26101 tgttgcgcga cggaataggc cacgttagtg tcgaaataga agctcttatc gccctctttc
    26161 cacacttcct gacccaattt taattcagca taagtttcac attcgttgcc aagacggtat
    26221 ttactttgag caccggtagt ctggaaacac tgttgttcac cgccgctacc tgtccagcca
    26281 ataccggaac gtgcatagcc gtggaaatca acagccattg cctgagcaga cattacgccc
    26341 gctgcgacgg caaccgccag aggaagtttg cgcagagtaa tcatcattct atctcctgag
    26401 tcattgcttt tcttttttca catcacctgt gacaggcttt gtgtgttttg tggggtgctt
    26461 aaacacccgg ctccttatgc agtcgacgac atgcagtgcc atcctcacgg aacagatggc
    26521 aacgctctgg cggcaggccg atagcgaatg tggcaccttc ttctaccaac accacgtcgt
    26581 tctggcggta caccaggttt tgacgaatgg aagggatctg gatatagatt tgggtttcgt
    26641 tgccgagttg ctcgacgacc tgaacttcac cctcaaggat gacgtcagcg atatcactcg
    26701 gcagtagatg ttccgggcga atacccagcg acatattggc tccaacctgg acatcacggc
    26761 tttcaacagg cagccagact tgttgacgat ttggcatcgg cagctccacc tgcacttgat
    26821 cgattgcggt ggcggtcact tttaccggca ggaagttcat ctttggcgaa ccgataaatc
    26881 cggcgacaaa acggtctgcc ggatagtggt acagttccag cggtttccca acctgtgcca
    26941 cgcgaccggc gtccagcacc acgattttgt cggccagcgt catcgcttcg acctgatcgt
    27001 gggtgacgta aatcattgtg cggcccaggc gtttatgcag acgggagatt tcgatacgca
    27061 tttgcacacg cagtgcagca tcgaggttgg agagcggttc atcgagcaaa aatacgcttg
    27121 gctcggccac cagcgtacgg ccaatcgcca cgcgctgacg ctgaccaccg gagagcgctt
    27181 tcggtttgcg atccagcaaa tgcgccagtt gcagcacttc cgccacctgg ttgacgcgtt
    27241 ggttaatcac ctcttttttt gcgccagcca gtttcaggcc aaatgacatg ttttctgcta
    27301 ctgacaggtg gggatagagc gcgtaagact gaaacaccat accaacgccg cgttctgctg
    27361 gcggagtgtc attcatccgt ttctcaccga tgaacaggtc gccgctggtg atcgtctcaa
    27421 gcccggcaat catgcgcagt aaagtcgatt taccgcagcc agacggtccg acaaacacca
    27481 cgaattcacc ttcatggata tcgagattga tatctttcga taccacgacc tcgccccagg
    27541 ctttcgttac attttgcagc tgtacgctcg ccatgccctt ctccctttgt aacaacctgt
    27601 catcgacagc aacattcatg atgggctgac tatgcgtcat caggagatgg cttaaatcct
    27661 ccaccccctg gcttttttat gggggaggag gcgggaggat gagaacgcgg cttctgtgaa
    27721 ctaaaccgag gtcatgtaag gaatttcgtg atgttgcttg caaaaatcgt ggcgatttta
    27781 tgtgcgcatc cccacattac cgccaattct gtaacagaga tcacacaaag caacggtggg
    27841 gcgtaggggc aaggaggatg gaaagaggtt gccgtataaa gaaactagag tccgtttagg
    27901 tgttttcacg agcacttcac caacaaggac catagattat gaaaataaaa acaggtgcac
    27961 gcatcctcgc attatccgca ttaacgacga tgatgttttc cgcctcggct ctcgccaaaa
    28021 tcgaagaagg taaactggta atctggatta acggcgataa aggctataac ggtctcgctg
    28081 aagtcggtaa gaaattcgag aaagataccg gaattaaagt caccgttgag catccggata
    28141 aactggaaga gaaattccct caggttgcgg caactggcga tggccctgac attatcttct
    28201 gggcgcacga ccgctttggt ggctacgctc aatctggcct gttggctgaa atcaccccgg
    28261 acaaagcgtt ccaggacaag ctgtatccgt ttacatggga tgccgtacgt tacaacggca
    28321 agctgattgc ttacccaatc gctgttgaag cgttatcgct gatttataac aaagacctgc
    28381 tgccgaaccc gccaaaaacc tgggaagaga tcccggcgct ggataaagaa ctgaaagcga
    28441 aaggtaagag cgcgctgatg ttcaacctgc aagaaccgta cttcacctgg ccgctgattg
    28501 ctgctgacgg gggttatgcg ttcaagtatg aaaacggcaa gtacgacatt aaagacgtgg
    28561 gcgtggataa cgctggcgcg aaagcgggtc tgaccttcct ggttgacctg attaaaaaca
    28621 aacacatgaa tgcagacacc gattactcca tcgcagaagc tgcctttaat aaaggcgaaa
    28681 cagcgatgac catcaacggc ccgtgggcat ggtccaacat cgacaccagc aaagtgaatt
    28741 atggtgtaac ggtactgccg accttcaagg gtcaaccatc taaaccgttc gttggcgtgc
    28801 tgagcaccgg tattaacgcc gccagtccga acaaagagct ggcgaaagag ttcctcgaaa
    28861 actatctgct gactgatgaa ggtctggaag cggttaataa agacaaaccg ctgggtgccg
    28921 tagcgctgaa gtcttacgag gaagagttgg cgaaagatcc acgtattgcc gccaccatgg
    28981 aaaacgccca gaaaggtgaa atcatgccga acatcccgca gatgtccgct ttctggtatg
    29041 ccgtacgtac tgcggtgatc aacgccgcca gcggtcgtca gactgtcgat gaagccctga
    29101 aagacgcgca gactcgtatc accaagtaat gctgtgaaat gccggatgcg gcgtgaacgc
    29161 cttgtccggc ctacaaaact gaaacgcatg taggcctgat aagacgcatc agtgtcgcat
    29221 caggcagttg ttgtcggata aggcgtgaaa gccttatccg tcctggaatg aggaagaacc
    29281 ccatggatgt cattaaaaag aaacattggt ggcaaagcga cgcgctgaaa tggtcagtgc
    29341 caggtctgct cggcctgctg gtgggttacc ttgttgtttt aatgtacgca caaggggaat
    29401 acctgttcgc cattaccacg ctgatattga gttcagcggg gctgtatatt ttcgccaatc
    29461 gtaaagccta cgcctggcgc tatgtttacc cgggaatggc tggaatggga ttattcgtcc
    29521 tcttccctct ggtctgcacc atcgccattg ccttcaccaa ctacagcagc actaaccagc
    29581 tgacttttga acgtgcgcag gaagtgttgt tagatcgctc ctggcaagca ggcaaaacct
    29641 ataactttgg tctttacccg gcgggcgatg agtggcaact ggcgctcagc gacggcgaaa
    29701 ccggcaaaaa ttacctctcc gacgcgttta aatttggcgg cgagcaaaaa ctgcaactga
    29761 aagaaacgac cgcccagccc gaaggcgaac gcgcgaatct gcgcgtgatt acccagaatc
    29821 gtcaggcgct gagtgacatt accaccattc tgccggatgg caacaaagtg atgatgagct
    29881 ccctgcgcca gttttctggc acgcagccgc tctacacact cgacggtgac ggcacgttga
    29941 cgaataatca gagtggcgtg aaatatcgtc cgaataacca aattggcttt taccagtcca
    30001 ttaccgccga cggcaactgg ggggatgaaa agctaagccc cggttacacc gtgaccaccg
    30061 gctggaaaaa ctttacccgc gtctttaccg acgaaggcat tcagaaaccg ttcctcgcca
    30121 ttttcgtctg gaccgtggtg ttctcgctga tcactgtctt tttaacggtg gcggtcggca
    30181 tggttctggc atgtctggtg cagtgggaag cgttgcgcgg caaagcggtc tatcgcgtcc
    30241 tgctgattct gccctacgcg gtgccatcgt tcatttcaat cttgattttc aaaggattgt
    30301 ttaaccagag cttcggtgaa atcaacatga tgttgagcgc gctgtttggc gtgaagcccg
    30361 cctggttcag cgatccgacc accgctcgca cgatgctgat tatcgtcaat acctggctgg
    30421 gttatccgta catgatgatc ctctgcatgg gcttgctgaa agcgattccg gacgatttgt
    30481 atgaagcctc agcaatggat ggcgcaggtc cgttccagaa cttctttaag attacgctgc
    30541 cgctgctgat taaaccgctg acgccgctga tgatcgccag cttcgccttt aactttaaca
    30601 acttcgtgct gattcagctg ttaaccaacg gcggcccgga tcgtcttggc acgaccacgc
    30661 cagccggtta taccgacctg cttgttaact acacctaccg tatcgctttt gaaggcggcg
    30721 ggggtcagga cttcggtctg gcggcagcaa ttgccacgct gatcttcctg ctggtgggtg
    30781 cgctggcgat agtgaacctg aaagccacgc gaatgaagtt tgattaaggg agataacaaa
    30841 aatggcaatg gtccaaccga aatcgcaaaa agcacgtttg tttattactc acctgctact
    30901 gctacttttt atcgcggcga ttatgttccc gctgctgatg gtcgtcgcta tctcgctgcg
    30961 tcagggaaac ttcgcgaccg gtagcctgat cccggagcaa atctcctggg atcactggaa
    31021 actggcgtta ggttttagcg ttgaacaggc tgatggtcgc attacgccac cgccattccc
    31081 ggtactgctg tggctgtgga actcggtaaa ggtcgctggg atttccgcga ttggcattgt
    31141 ggcgctctcc accacctgcg cctacgcttt cgcccgtatg cgctttccag gcaaagcgac
    31201 gctgctgaaa ggaatgctga ttttccagat gttcccggca gtactttcac tggtcgcgtt
    31261 gtatgcgttg tttgatcgtc tgggtgagta cattccattc attggcctga atactcacgg
    31321 cggcgtaatt ttcgcgtatc tgggtgggat tgcgctgcat gtctggacca tcaaaggcta
    31381 tttcgaaacc atcgacagtt cgctggaaga agcggctgcg ctggatggtg cgacaccctg
    31441 gcaggccttc cgccttgtcc tgttgccgct gtcagtaccg attctggcgg tggtattcat
    31501 cctgtcgttt atcgctgcca ttactgaagt tccggtcgcg tcgctgttac tgcgtgacgt
    31561 aaacagctac accctggccg tggggatgca gcaatacctc aacccgcaaa actacctgtg
    31621 gggtgacttt gccgccgctg ccgtgatgtc tgcattaccg atcaccatcg tcttcttgct
    31681 ggctcaacgc tggctggtca acggcctgac ggcaggtggt gt
//
LOCUS       DAJVQH010000035        31316 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJVQH010000035 DAJVQH010000000
VERSION     DAJVQH010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.035"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(27..281)
                     /locus_tag="OQM87_001682"
     CDS             complement(27..281)
                     /locus_tag="OQM87_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCS1507848.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            436..1008
                     /gene="yeiP"
                     /locus_tag="OQM87_001683"
     CDS             436..1008
                     /gene="yeiP"
                     /locus_tag="OQM87_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCS1507849.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1231..2697
                     /locus_tag="OQM87_001684"
     CDS             1231..2697
                     /locus_tag="OQM87_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1507850.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNPLKI"
     gene            2815..3801
                     /gene="yeiR"
                     /locus_tag="OQM87_001685"
     CDS             2815..3801
                     /gene="yeiR"
                     /locus_tag="OQM87_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCS1507851.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3840..4553
                     /gene="lpxT"
                     /locus_tag="OQM87_001686"
     CDS             3840..4553
                     /gene="lpxT"
                     /locus_tag="OQM87_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCS1507852.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4965..5531
                     /gene="mepS"
                     /locus_tag="OQM87_001687"
     CDS             4965..5531
                     /gene="mepS"
                     /locus_tag="OQM87_001687"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCS1507853.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5712..7268
                     /locus_tag="OQM87_001688"
     CDS             5712..7268
                     /locus_tag="OQM87_001688"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS1507854.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7350..9164
                     /locus_tag="OQM87_001689"
     CDS             7350..9164
                     /locus_tag="OQM87_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1507855.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9165..10259
                     /locus_tag="OQM87_001690"
     CDS             9165..10259
                     /locus_tag="OQM87_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCS1507856.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10259..11284
                     /locus_tag="OQM87_001691"
     CDS             10259..11284
                     /locus_tag="OQM87_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1507857.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11286..12875
                     /gene="yejF"
                     /locus_tag="OQM87_001692"
     CDS             11286..12875
                     /gene="yejF"
                     /locus_tag="OQM87_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCS1507858.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     LSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12879..13223)
                     /locus_tag="OQM87_001693"
     CDS             complement(12879..13223)
                     /locus_tag="OQM87_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507859.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13556..14746)
                     /gene="bcr"
                     /locus_tag="OQM87_001694"
     CDS             complement(13556..14746)
                     /gene="bcr"
                     /locus_tag="OQM87_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCS1507860.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14774..15469)
                     /gene="rsuA"
                     /locus_tag="OQM87_001695"
     CDS             complement(14774..15469)
                     /gene="rsuA"
                     /locus_tag="OQM87_001695"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCS1507861.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15619..17379
                     /locus_tag="OQM87_001696"
     CDS             15619..17379
                     /locus_tag="OQM87_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1507862.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17504..17788
                     /gene="rplY"
                     /locus_tag="OQM87_001697"
     CDS             17504..17788
                     /gene="rplY"
                     /locus_tag="OQM87_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCS1507863.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17927..18934)
                     /gene="yejK"
                     /locus_tag="OQM87_001698"
     CDS             complement(17927..18934)
                     /gene="yejK"
                     /locus_tag="OQM87_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCS1507864.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19116..19343
                     /locus_tag="OQM87_001699"
     CDS             19116..19343
                     /locus_tag="OQM87_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCS1507865.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19363..21123
                     /gene="pbgA"
                     /locus_tag="OQM87_001700"
     CDS             19363..21123
                     /gene="pbgA"
                     /locus_tag="OQM87_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCS1507866.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21198..21274
                     /locus_tag="OQM87_001701"
     tRNA            21198..21274
                     /locus_tag="OQM87_001701"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21232..21234,aa:Pro,seq:ggg)
     gene            complement(21377..23968)
                     /gene="yejO"
                     /locus_tag="OQM87_001702"
     CDS             complement(21377..23968)
                     /gene="yejO"
                     /locus_tag="OQM87_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCS1507867.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24288..24935
                     /gene="narP"
                     /locus_tag="OQM87_001703"
     CDS             24288..24935
                     /gene="narP"
                     /locus_tag="OQM87_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCS1507868.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25146..26198)
                     /gene="ccmH"
                     /locus_tag="OQM87_001704"
     CDS             complement(25146..26198)
                     /gene="ccmH"
                     /locus_tag="OQM87_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCS1507869.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26195..26752)
                     /gene="dsbE"
                     /locus_tag="OQM87_001705"
     CDS             complement(26195..26752)
                     /gene="dsbE"
                     /locus_tag="OQM87_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCS1507870.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26749..28692)
                     /gene="ccmF"
                     /locus_tag="OQM87_001706"
     CDS             complement(26749..28692)
                     /gene="ccmF"
                     /locus_tag="OQM87_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCS1507871.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28689..29168)
                     /gene="ccmE"
                     /locus_tag="OQM87_001707"
     CDS             complement(28689..29168)
                     /gene="ccmE"
                     /locus_tag="OQM87_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCS1507872.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29165..29374)
                     /gene="ccmD"
                     /locus_tag="OQM87_001708"
     CDS             complement(29165..29374)
                     /gene="ccmD"
                     /locus_tag="OQM87_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCS1507873.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29371..30108)
                     /gene="ccmC"
                     /locus_tag="OQM87_001709"
     CDS             complement(29371..30108)
                     /gene="ccmC"
                     /locus_tag="OQM87_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCS1507874.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30150..30812)
                     /gene="ccmB"
                     /locus_tag="OQM87_001710"
     CDS             complement(30150..30812)
                     /gene="ccmB"
                     /locus_tag="OQM87_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCS1507875.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30809..>31316)
                     /gene="ccmA"
                     /locus_tag="OQM87_001711"
     CDS             complement(30809..>31316)
                     /gene="ccmA"
                     /locus_tag="OQM87_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCS1507876.1"
                     /translation="AGKTTLLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGH
                     QPGIKTRLTALENLHFYHRDGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALAR
                     LWLTRATLWILDEPFTAIDVNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRI
                     SLTQTRAA"
BASE COUNT         7752 a         8331 c         7684 g         7549 t
ORIGIN      
        1 ttttgcttac tgcggattaa agatgtttaa ggcgcggtca gcatttccag atcgataagc
       61 caggtcatgg cctgctgacg cgagttaccg cacatttcag cgctggcttg taagcctgca
      121 cagactttcg gtcgcagtgg cgaggtgaaa attttacagc gttgctgttc atcaagctga
      181 atgcagggcg tattggcggg cttgccatct ggcataccgg gaatagggct ggaaattgaa
      241 ggggcagtgc aacacgcccc gcaacccgga cggcattcca tacgttgttc tcttaaggtt
      301 aaccaccgga ttgatgagcg cgcacagtac cacctttttt gcaccagcaa aagtgcgaat
      361 accacttgcc cgaaaggccc atcgcgagta ctttgtcgcg atatttttga cattttcgac
      421 tacaggaatt tttcgatgcc aagagcgaac gaaattaaaa aaggtatggt actgaattac
      481 aacggcaaac tgctgctggc taaggatatt gatattcagt cgcccactgc ccgcggtgcc
      541 gctacgctgt acaaaatgcg tttttctgat gtccgtaccg ggctgaaagt agaagagcgt
      601 ttcaaaggtg atgatatcgt tgacaccgtg acgctgaccc gccgttacgt tgacttctcc
      661 tatgtcgatg gcaacgaata tgtctttatg gataaagaag actatacccc gtataccttc
      721 accaaagatc agattgaaga agagttgctg tttatgccag agggcggcat gccggacatg
      781 caggtgctga cctgggacgg tcaactgctg gcgcttgagc tgccgcagac cgttgatctg
      841 gaaatcgttg aaaccgcacc aggcatcaaa ggggcatctg ccagcgcccg taacaaaccg
      901 gcgacattga gcactggtct ggtgattcag gtaccagaat acttaagccc gggcgaaaaa
      961 attcgtatcc atatcgaaga acgccgttat atggggcgtg ctgactaact tcagccgcat
     1021 gcagaaaagg gatagctcag gctgtccctt ttttaattta ttataccaat cttctatttt
     1081 gcgcttcctg aacaccttac gccctcaatt ttcactcgtt gattgatcgc cctcacactt
     1141 catcgcatta acaatccaga ccaatttcaa ttgctgtcat ataactttac actgtcattg
     1201 ttaatttatc gttactaaga cgtgactcct atgaagacaa ttgcctccgt tacgctcccg
     1261 catcatgtac acgctccacg ctacgatcgc cagcagttgc aatcacgtat cgttcatttt
     1321 ggctttggcg cctttcaccg cgctcatcag gcgttactga ccgatcgtgt gctgaatgcc
     1381 cagggcggcg actgggggat ctgtgaaatc agcttgttca gcggtgatca actgatgagc
     1441 cagctccgcg cacagaacca tttatatacc gtgctggaga aaggtgcgga cggcaatcag
     1501 gtgataattg tcggtgccgt tcacgaatgc cttaatgcaa aactggattc cttagcggca
     1561 attattgaga aattttgcga gccacaggtg gcaattgttt ccctgacgat taccgaaaaa
     1621 ggctattgta ttgacccggc caccggtgca ctcgacacca gtaatccgcg gattattcac
     1681 gatctacaaa cccctgaaga acctcactcc gcaccgggta ttctcgtcga agcactgaaa
     1741 cgccgccgtg agcgcggcct tacaccgttt accgtgctct cctgcgacaa tattcccgac
     1801 aatggccatg tggtgaaaaa cgcggtgctg agaatggcag aaaaacgttc gccagaactc
     1861 gccgggtgga taaaagaaca cgtcagtttt ccgggaacca tggtcgaccg cattgttccg
     1921 gctgcaaccg acgaatcact ggcagaaatc agccagcatc tgggggtaaa tgatccctgc
     1981 gcgattagct gcgaaccgtt tatccagtgg gtggttgaag ataacttcat cgctgggcgt
     2041 cctgcctggg aagtcgcagg tgtacaaatg gtgaatgatg tcctgccatg ggaagagatg
     2101 aaactgcgta tgcttaatgg cagccactct tttctcgctt atctgggtta cctctcagga
     2161 ttcgcctata tcagtgattg catgcaggat cgcgtatttc gctatgctgc cagaacatta
     2221 atgctggatg agcaagcgcc gacactgcaa attaaagatg tcgatttaac acaatatgcg
     2281 gataagttaa ttgcacgttt tgctaatccg tcgctgaaac ataagacctg gcaaatcgcg
     2341 atggatggca gccagaaatt accgcaacgt atgctggcag gtattcgcat acatctgggg
     2401 cgtgaaacgg actggtcgtt gctggcatta ggcgttgctg gctggatgcg ttacgtcagc
     2461 ggcgttgatg atgccggaaa tgccattgat gttcgcgatc cgcttagcga taaaattcgc
     2521 gaacttgttg cgggcagcag cagtgaacaa cgcgtaaccg ccctgctttc cctgcgtgaa
     2581 gttttcggtg atgatctgcc agataacccg cattttgtgc aggccatcga acaagcctgg
     2641 caacaaatcg tacaattcgg cgcacatcag gcgctattaa accccctcaa aatttaacga
     2701 tttctgcggt taaagcggat gaagctcacc ttcgtccgct ctccccttct cttttctgcc
     2761 ttttttagcc aggattaacg ctcagttaac ttaccagagt attgcggagc cgttgtgacc
     2821 aggaccaacc tcatcaccgg ttttctcggc agcgggaaaa ccacgtcgat tcttcatctg
     2881 ttagcccata aagatcccaa cgaaaaatgg gcggtactgg ttaatgaatt tggggaagtc
     2941 ggaattgatg gtgctttgct cgccgatagc ggcgcattgc tgaaagagat ccccggcggc
     3001 tgcatgtgct gcgttaatgg tttacccatg caggtagggt tgaatacctt actgcgtcag
     3061 ggaaaaccag accgcttgtt gatagagccg accgggctgg gccatccgaa acagctcctc
     3121 gatctgttaa ccgcaccagt ctatgaaccg tggatagatc tgcgcgccac cttgtgcatt
     3181 ctcgatccac gcctgctgct ggacgaaaaa agcgccagca atgaaaactt ccgtgaccag
     3241 ctggctgccg cagacatcat tgtcgccaat aaatccgacc gtgcgacgcc cgaaagtgag
     3301 caagcgctac agcgttggtg gcagcaaaat ggtggcgatc gacaattaat tcacagtgag
     3361 catgggaaag ttgacggtca tcttctggat ttgccgcgtc gcaatttagc cgagttgccc
     3421 gccagcgccg cgcattctca tcagcatgtc gtgaaaaaag ggttagcagc gttaagcctg
     3481 ccagagcatc aacgctggcg tcgcagtctg aacagcgggc aaggatatca ggcctgcggc
     3541 tggatattcg acgctgatac ggtattcgac accattggca ttctggaatg ggcgcgactt
     3601 gcaccggtgg aacgcgtcaa aggcgtgctg cgtattcccg aagggctggt acgaatcaac
     3661 cgtcagggcg atgacctgca cattgaaacg caaaacgttg cgccaccgga cagccgtatt
     3721 gagctgattt ccagcagcga agctgactgg aatgccttgc agagcgcgct gttgaagctt
     3781 cgtttagcga ctaccgcgta aggttgcctg cgtttttcag taagataatt agagaaaata
     3841 tgattaaaaa tttgccgcaa atagtgttgt tgaatattgt cggccccgcg ctgtttcttt
     3901 cctggtatat tcccgttaat catggattct ggttgccgat tgatgcggat attttttatt
     3961 tctttaatca gaaactggtc gaaagtaagg cctttttatg gctggttgca ttgactaaca
     4021 atcgcgcctt cgacggttgt tcactgctgg cgatgggtat gttgatgctg agtttctggc
     4081 tgaaagaaaa cgcccctggc agacgacgta tcgtgattat gggtctggtc atgctattaa
     4141 ctgcagtggt attaaaccag ctgggtcagg cattaattcc tgtaaaacgg gccagcccaa
     4201 cattgacttt taccgatatt aaccgcgtca gcgaactgct ctctgttccc acgaaagatg
     4261 cctcacgaga tagctttccc ggcgatcacg gcatgatgct gcttattttt tcggcattca
     4321 tgtggcgtta tttcggcaaa gttgcaggcc ttatcgccct tattattttt gtggtttttg
     4381 catttcccag agtaatgatt ggcgcacact ggtttactga catcattgtc ggttcgatga
     4441 ccgtgatatt gatcggtttg ccctgggtgt tgctgacgcc attaagtgat cgattaatca
     4501 ccttttttga caaatcacta ccaggaaaaa acaaacattt ccaaaacaaa taactcacag
     4561 taattaacat catcagggtt atttttatag tgaggataat cctgatgatg cgcaccgtgc
     4621 tttcatctat cgaacgcaaa aatcattctc taagtaaatg aatggattgc atgcgtttca
     4681 ctcaattgta ctttaattga ccaaccccgc ttattaactt tctgtatcac tttttcttat
     4741 aaaaaatcat gtaaaaccgc tcgccaagac cgcaccaatc gggtaatctc gaactcgttt
     4801 tgcctcggcg gtagattatc ctcacagcat ataattttgt gcgttagtcc acagatttgg
     4861 ccttaaggaa ttgtttcaac atgcccaggt aattagtctc gtgtcgcctg gcattttttt
     4921 ataacgatat ttgtcgttaa ggacttcaag ggaaaacaaa caacatggtc aaatctcaac
     4981 cgattttgag atatatcttg cgcgggattc ccgcgattgc agtagcggtt ctgctttctg
     5041 catgtagtgc aaataacacc gcaaagaata tgcatcctga gacacgtgca atgggtagtg
     5101 aaacatcatc actgcaagct tctcaggatg aatttgaaaa cctggttcgt aatgtcgacg
     5161 taaaatcgcg aattatggat cagtatgctg actggaaagg cgtacgttat cgtctgggcg
     5221 gcagcactaa aaaaggtatc gattgttctg gtttcgtaca gcgtacattc cgtgagcaat
     5281 ttggcttaga acttccgcgt tcgacttacg aacagcagga aatgggtaaa tctgtttccc
     5341 gcagtaattt gcgtacgggt gatttagttc tgttccgtgc cggttcaacg ggacgccatg
     5401 tcggtattta tatcggcaac aatcagtttg tccatgcttc caccagcagt ggtgttatta
     5461 tttccagcat gaatgaaccg tactggaaga agcgttacaa cgaagcacgc cgggttctca
     5521 gccgcagcta ataaaccgtt tggatgcaat cccttggcta tcctgacgag ttaactgaaa
     5581 gcactgctta ggcagtgctt ttttgttttc attcatcaga gaaaatgatg tttccgcgtc
     5641 ttgatccagg ctatagtccg gtcattgtta tcttttaaat gttgtcgtaa tttcaggaaa
     5701 ttaacggaat catgttcata cgcgctccca attctggacg taagctcctg cttacctgca
     5761 ttgtagcagg cgtgatgatt gcgatactgg ttagctgcct tcagttttta gtggccaggc
     5821 ataagcacga agtcaaatac gacacactga ttatcgacgt acaaaagtat ctcgatacct
     5881 attttgccga cctgaaatcc actactgacc ggcttcagcc gctgacctta gatacctgcc
     5941 agcaggctaa ccccgaactg accgctcgcg cggcgtttag catgaatgtc cgtacgtttg
     6001 tgctggtgaa agataaaaaa acattctgtt catctgcgac tggtgagatg gacattccac
     6061 tcaatgaatt gattccggcg ctcgacatta ataaaaacgt cgatatggcg atcttacccg
     6121 gcacgccgat ggtgccgaac aaacccgcaa tcgtcatctg gtatcgcaac cctttgctga
     6181 aaaatagcgg tgtgtttgcc gctctgaatc tcaacctgac gccttctctc ttttatagtt
     6241 cacggcagga agattacgat ggcgtcgccc tcattattgg caatactgcg ctatctacct
     6301 tttcttcacg tttgatgaac gttaatgaat taaccgacat gccggtccgt gaaactaaaa
     6361 ttgcgggcat tcctctgacc gttcggcttt atgcggatga ctggacatgg aacgatgtgt
     6421 ggtacgcatt tttactgggc ggcatgagtg gaactgtcgt tggcctgctt tgctattacc
     6481 tgatgagcgt acgtatgcgc cccggcagag aaatcatgac cgccatcaag cgcgaacaat
     6541 tttacgtggc gtatcaaccg gtggtggata cacaagcttt gcgggtaacg ggcctggaag
     6601 tactgctacg ctggcggcat cctgtagcgg gagaaattcc cccggatgcc ttcattaact
     6661 ttgccgaatc gcaaaagatg attgtgccgc tgactcagca cctgtttgag ttaattgccc
     6721 gcgatgccgc agaattagaa aaagtactgc cggtaggcgt caaatttggc attaacattg
     6781 cgccggacca tctgcacagc gaaagcttta aagcagatat ccagaaactg ctcacttccc
     6841 tgcccgcaca ccatttccag attgtgctgg aaattaccga gcgcgatatg ctgaaagagc
     6901 aagaagccac acaactcttc gcctggctgc acttggtcgg cgtagaaatt gctattgatg
     6961 acttcggcac cgggcacagc gcgcttatct atcttgagcg ttttacgctc gattatctga
     7021 aaattgaccg tggatttatc aacgccatcg gtacggaaac gatcacttca cccgtacttg
     7081 acgcggtgct gacgctggcg aaacgtctca atatgctgac agttactgaa ggggtcgaaa
     7141 cgccggaaca agcgcgatgg ctaagcgaac gcggcgttaa tttcatgcaa ggctactgga
     7201 ttagtcgccc gttaccgctg gacgattttg ttcgctggct gaagaaaccg tatacgccgc
     7261 agtggtaagg tgtgcttacg tcccttatta ttcatagtga aagcatgccg gattgcggct
     7321 aatgatgagt aaaaggaaat ccgttgcaga tgattgtgcg catactgctg ctgtttatcg
     7381 ctctgttcac cttcggtgcg caggcgcagg ctatcaagga aagctatacc tttgccgtgc
     7441 tgggcgaacc ccggtacgcg tttaatttca accattttga ttatgtgaac cccgccgcgc
     7501 caaaaggtgg gcagataacg ttgtcagccc tcggtacctt cgataatttc aaccgctatg
     7561 cactgcgcgg caacccgggc gcacgcaccg agcagctgta cgacacgtta tttacgactt
     7621 ccgatgacga accaggcagt tattacccgc tgattgctga aagtgcacgc tatgctgacg
     7681 attattcctg ggtggaggtc gctattaatc cgcgcgcccg ttttcatgat ggttctccca
     7741 ttactgcccg cgatgtagag tttacttttc aaaaatttct gaccgaaggc gtgccgcaat
     7801 ttcgtctggt ctacaaaggc accaccgtca aagccattgc gccgttaacc gtgcgcattg
     7861 agttagctaa acccggcaaa gaagatatgt tgagtctgtt ttcgctgccg gtatttccag
     7921 aaaagtactg gaaggatcac aaacttagcg acccgctcgc cacgcctccg cttgccagtg
     7981 gtccgtacca cgttacgtcc tggaaaatgg ggcaaaatat tgtctattcc cgcgtgaaag
     8041 attactgggc agcaaactta ccggtaaacc gtggacgctg gcatttcgac accattcgct
     8101 acgattatta cctcgatgat aatgtcgcct ttgaagcgtt taaagcaggt gcctttgatt
     8161 tgcgtatgga aaacgacgct aaaaactggg ccacgcgtta taccggtaaa aatttcgata
     8221 aaaaatacat catcaaagat gagcaaaaga acgaatcagc ccaggatacg cgctggctgg
     8281 cgtttaatat ccaacgtgcg gtattcagcg atcgccgggt gcgggaagca atcactctcg
     8341 cctttgactt tgaatggatg aacaaggcgt tgttttacaa cgcctggagt cgcacgaaca
     8401 gttattttca aaataccgaa tacgcggcca gaaattaccc cgacgccgcg gagctggtgc
     8461 ttctggcacc aatgaaaaaa gatctaccgc cagaagtctt cacacaaatc taccagccgc
     8521 cggtatctaa aggcgatggc tacgatcgtg acaacctgtt aaaagccgac aaacttctca
     8581 acgaagcggg ctgggtgctg aagggtcagc aacgcgttaa tgccacaacg ggtcagccgc
     8641 tcagctttga attattgctt cccgcaagca gcaatagtca gtgggtattg ccgttccagc
     8701 acagcctgca acggctgggt atcaatatgg acattcgcaa ggtggataac tcgcaaatca
     8761 ctaaccgcat gcgcaatcgc gactatgaca tgatgccgcg cctatggcgg gcgatgccgt
     8821 ggcccagttc cgatttacag atttcctggt catcggaata tatcaattcc acttataatg
     8881 cccccggcgt gcaaagcccg gttatcgact cgctgatcaa ccaaattatt gccgcgcagg
     8941 gaaataaaga aaaattactg ccgttggggc gagcactgga tcgcgtatta acgtggaatt
     9001 attacatgct gccaatgtgg tacatggcgg aagaccgtct cgcctggtgg gataaattct
     9061 cccaacccgc tgtacgccct gtttacagcc taggtatcga tacctggtgg tatgacgtta
     9121 ataaagcggc caaactgccg tcagccagac aacagggaga gtagatgggc gcttacctga
     9181 ttcgccgtct gttgctggtg atcccaacgt tatgggctat tatcactatc aactttttca
     9241 tcgtgcaaat tgcgcctggc ggtccggtcg atcaggccat cgccgccatt gagtttggta
     9301 atgccggagt attacccggc gcaggcggtg aaggtgttcg tgccagccat gcgcaaacgg
     9361 gtgtcggcaa tatcagcgac agtaattacc gtggcggacg cggattagat ccagaagtga
     9421 tcgctgagat cactcatcgc tacggtttcg ataagccgat ccacgaacgt tacttcaaaa
     9481 tgctctggga ctacatccgc tttgattttg gtgatagcct gtttcgcagc gcgtcggtgc
     9541 tgacgctgat taaagacagt ctgccggttt ccatcaccct cggattgtgg agcacgctga
     9601 ttatctatct ggtgtcgatt ccgttaggca ttcgcaaagc tgtttataat gggagtcgct
     9661 ttgacgtctg gagtagcgca tttatcatca tcggctacgc cattccggcc tttttgtttg
     9721 ccatcctgct gattgtcttc ttcgcgggcg gcagctattt cgacctgttc cctctacgcg
     9781 gcctggtttc cgctaacttt gattcgctgc cgtggtatca gaaaatcacc gattatctgt
     9841 ggcatatcac gctaccggtg ctggcgacag tgattggtgg ctttgcggcg ctgaccatgc
     9901 tgaccaaaaa ctcattcctt gatgaagtgc gcaagcaata cgtggtgacc gcgcgtgcga
     9961 aaggggtaag tgagaaaaac attctctgga aacatatgtt ccgcaacgcc atgctgctgg
    10021 tgattgccgg ttttccggcg acgtttatca gcatgttttt taccggctcg ctgctgattg
    10081 aggtgatgtt ttcactcaat ggtcttggct tactgggcta cgaagcgacc gtctcgcgcg
    10141 attatcctgt aatgtttggt accttgtata ttttcaccct gattggcctg ctgctgaata
    10201 ttgtcagtga tatcagctat acgctggttg atccgcgtat agattttgag ggacgttaat
    10261 gtcgcgactc agccccgtca atcaggcccg ttgggcgcgt tttcgtcata accgtcgcgg
    10321 ctactggtcg ttatggattt tcctcgtttt gtttggtttg agtttgtgtt ctgaacttat
    10381 cgctaacgat aaaccgttgc tggtgcgtta tgacggcagt tggtatttcc cgttgttgaa
    10441 aaactacagc gaaagcgatt ttggcggccc gctggcaagt caggctgatt atcaggaccc
    10501 gtggctgaaa caacggctgg aaaataacgg ctgggtactg tgggcaccga ttcgctttgg
    10561 tgctaccagt atcaactttg ctaccgataa gcccttccct tctccaccct cccggcaaaa
    10621 ctggctggga acggatgcaa acggcggcga tgtgctggca cgcattctct atggcacgcg
    10681 gatctcggtt ctgtttggcc tgatgctgac tctctgttcc agcgtgatgg gcgtgctggc
    10741 gggggcgcta caaggctatt acggcggtaa agtcgatctc tgggggcaac gctttattga
    10801 agtatggtcg gggatgccga cgctgttttt gattatttta ctttccagcg tcgtacagcc
    10861 taacttctgg tggctgctgg caattactgt cttgtttggc tggatgagtc tggtcggcgt
    10921 ggtgcgggcg gagtttttac gtactcgtaa tttcgactac attcgtgcgg cacaggcgct
    10981 tggcgtcagc gatcgcagta tcatcctgcg tcatatgttg cctaatgcca tggtcgcgac
    11041 cctcaccttt ttaccgttta ttttatgtag ttcgataacc accctgacct cgctcgattt
    11101 cctcggcttc ggtctaccgc tcggttcacc gtcactcggt gaactgctgt tacaagggaa
    11161 aaataacctt caggccccgt ggcttgggat caccgccttc ttgtcggtgg cgatattatt
    11221 gtctttgctg atctttattg gtgaagccgt ccgcgacgca tttgatccta ataaggcggt
    11281 gtagcatgac gcaaactctg ttagcgattg aaaatttgtc ggtgggtttt cgccatcagc
    11341 aaaccgtacg tacagtagtc aatgatgttt cactacagat tgaggctggc gaaacgctgg
    11401 cgctggtggg tgagtcaggt tcaggcaaaa gcgttaccgc gctgtcaatt ttacgcctgc
    11461 tcccttcccc gccggttgaa tatctctccg gcgatattcg ttttcatggc gaatcgctgc
    11521 ttcatgccag cgatcaaacg ttgcgcggtg tacgcggtaa taagatcgcc atgatttttc
    11581 aggaaccgat gctgtcgtta aatccattgc ataccctgga aaaacagctt tatgaagtgc
    11641 tttcactcca ccgcgggatg cgtcgggaag cggctcgtgg cgaaattctt aactgccttg
    11701 atcgcgtcgg tatccgccag gcggcaaaac ggctgacaga ttatccgcat cagctctccg
    11761 gcggcgaacg ccagcgggtg atgattgcga tggcgctgtt aacgcgaccg gaattattaa
    11821 ttgccgatga accgaccacc gcactggacg tctctgtcca ggcgcagatt ttacagctgt
    11881 tgcgcgaact gcaaggcgag ctgaatatgg gcatgctgtt tattactcat aacctcagca
    11941 ttgtcagaaa actggcccac cgcgtggcgg taatgcaaaa cggtcgctgt gtcgagcaaa
    12001 ataacgccgc tacgctattt gcctcaccca ctcatcctta cacacaaaag ctactcaaca
    12061 gtgaaccgtc aggcgatccg gtaccgttgc cagaacctgc ctcaacgttg ctggatgttg
    12121 aacagcttca ggttgccttc cccattcgca aagggatttt gaagcgcatt gtggatcata
    12181 atgtggtggt gaaaaacatc agttttacgc tacgggcggg tgaaacactg ggtttagtgg
    12241 gcgagtctgg ttccgggaaa agcacgacgg gactggcgct gctgcgactg attacctctc
    12301 agggcagcat cgtctttgac ggtcagccac tgcaagattt aaatcgccgc cagctgttac
    12361 ctactcgtca tcgtattcag gtggtatttc aggatccaaa ctcctcgctc aacccacgac
    12421 tcaacgtttt gcagattatt gaggaaggct tacgggttca ccagccgacg ctttctgccg
    12481 cacaacgcga acaacaagtg atagccgtga tgcatgaagt gggattagat cctgaaacac
    12541 gccaccgtta tccggcggag ttctctggtg gtcagcgaca acgtattgcg attgccaggg
    12601 cattaattct taagccctcg ctgatcatac ttgatgaacc gacatcatca ctcgacaaaa
    12661 cggtacaggc gcaaatattg acgcttttga aatcattgca acaaaagcat caactggcct
    12721 atttgtttat cagccacgat ttgcacgttg tccgcgcgtt atgtcatcag gttatcgtac
    12781 tgcgacaagg ggaagtagtg gaacaaggac cgtgcgcgcg cgtgtttgcc acaccgcagc
    12841 aggagtatac gcgtcagtta ctggcgttga gctgacgctt aaaaaggatt gtagtttgaa
    12901 aagggttcgg caattgccac accaaaattt ttcaatcggc atgttgcagc aaactcgtcc
    12961 tggcgattta caaacaggca cggctcacct tcacactcca acaccgaaca tggaacttcg
    13021 atgtcgctta acgcctggct tagtttatac atcaccgacc atgcattatc accatccggg
    13081 ctaagcaatt taagcgccac caggctgttg tcaccgcacg gtccattttg cggaatcggt
    13141 tcaaccttcg aacctgcgaa accagcagac cagcgatagt tctgcggcag tcgatggacg
    13201 attgagagtt gtaatgaagt cacttatttt ccccggaagt acattcgctt cacaatttgt
    13261 ttacatcaat tttaacacat catcaacaat ccgtctttat acagatcgta aagattgatg
    13321 cctcgcctgc gtggcctcta tggctctgat ttaagtaata tctgcgggcg tggtcacgtt
    13381 tgcggggcta tatgtacccg tttctgcgat ctaactcaac ctttttaact acaatgatgt
    13441 gactttttac ataaattgat tttacataaa ataaacatat atcggggaaa tgatagattt
    13501 gtggttgaca tgcatcaaca aaggaagcct tttaccttcc tcgttgtgca atagatcacc
    13561 gttttttcgg ccgactggcg tacagacaga agagaatgga gctggttgcg cagaatgcaa
    13621 ttgaccaaat catcggccat gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa
    13681 ttgcccctat gccaaaacgg aaggttcctg ccagcgaaga tgccgttccc gccatatggg
    13741 gaaactcatc aagaatgacc gccatcgcat tggatgacac catcgacacg cagcccacaa
    13801 acaccgcaac gccaaccacc agcgaccaga atcccagccc cagcagcgca ctgatgacca
    13861 tccacgctgc cataataaat tgtatccaca accccgagcg gaacatattt aacgcgccaa
    13921 tgcggcggac gaagcggctg ttaaagatgg tcatcacgaa cagaaaaaca atgtttagcg
    13981 caaagtaata accaaagttt tccggcgcga cgtggttaat ttcaatataa acaaacggtc
    14041 cggcgcttaa gaatgagaac atcccggcaa agctgaaacc actggcaagc atgtagctca
    14101 ggacacgttt atggcggaac agcgccgcaa agttaccaat agtggtacga atgtgaaatg
    14161 gctgacgacg ctccggtggt aaggtttctt taatcaggaa gaaaatcatt gccgaagcca
    14221 gaatcgccgc taatgccagg atccagaaga tgtaatgcca gctcagccac accagcaccc
    14281 agccgccaac tatcggtgcc atcagcagtg caatggtcgt caccagcatg acaaacgaca
    14341 tcatccgcga gaactcttct ttcgggtaaa tatcacgcat caaagcgttg ataaccacgc
    14401 tggccgcagc cgcagccagc ccgtggaaga aacgcatcac aatcagctga tcgatggttt
    14461 gtgccaacgc acacgccacc gcggcggcgg caaacaccag cgtcccgcca agaaccaccg
    14521 gcttacgccc gaagctgtct gccatcggcc cgtagattaa ctgccccaac gcaaagccca
    14581 gaatataagt actgagggtc atctgcgtac tgcccgccga tacgccaaac tgcgctgaaa
    14641 ttaccggtag cgcgggcaga tacatatcaa tcgacagcgg catcaacatg gccagcaggc
    14701 caaggataaa aacaatagca aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa
    14761 agtcattgaa aagttagacg acgctggcaa tttcttcttc agttaacgga cgatattcac
    14821 cgggggctaa atcagcatcc agcgtaatac cgccaatacg ttcacgatgc agctcaacca
    14881 cgtggttacc cacggcggcg aacatgcgtt tcacctgatg ataacgccct tcgctgatgg
    14941 tcagacgaac ctgcgttggg gtaatcactt caagcaccgc cggcttagtg agatcttttt
    15001 cgttatgcag ctgcacgcct ttagcaaatt gctctgccgt atcgtcagct acaggtgatt
    15061 ccagtgtcac cagataggtc ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc
    15121 actgaccatc atcagtcatc agcaccagac cggtggtatc gatatccaga cgtcctgcgg
    15181 cgtgcagttt ccacgctacc ggttcatcca gaaaatagag caccgtcggg tgatccgggt
    15241 cgtccgtgga gcaaacatag ccctgaggct tattgagcat gaagtaacgt ggaccgtgtt
    15301 gctgcgccag cgggttgcca tcgtaagcga catcatgttc agggagcagt ttgaacgctg
    15361 cattacgaac gatttcgcca tcgacggtga cacgattgcc gcggatttca cgcccggcaa
    15421 tagcacggct aacgccgagt tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt
    15481 tgcctgtaaa aaacggaggt cgggtaatga cccgaaaatc tgaactgttg tctgcccagt
    15541 atagcggtct aataacttcc ctcaagggaa aaagaaccgt ggcatactat tagcagaata
    15601 atctacctac gcgaaaccat gatttttaca ctccgcccat atcagcaaga agccgtggat
    15661 gccacgctca accattttcg tcgtcataaa acccctgccg ttatcgtgct gcccaccggc
    15721 gcaggtaaaa gcctggtgat agcggaactg gcacggctgg cgcgtggtcg cgtactggtg
    15781 ctggcacacg ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg
    15841 gaagccgata tttttgccgc cgggctaaag cgcaaagaga gccacggtaa agtggtattt
    15901 ggcagcgttc agtcggtcgc ccgtaatctt gatgcctttc agggtgaatt ttcgctgttg
    15961 attgtcgatg aatgccaccg tattggtgac gatgaagaga gccagtatca gcaaatcctc
    16021 actcacctga ctaaagtgaa tccccactta cgcctgctgg ggctgactgc cacgcctttt
    16081 cgattgggca aaggctggat ctaccagttc cattatcacg gcatggtacg cggcgatgag
    16141 aaagcccttt tccgtgactg catttatgag ctgccgctgc gttatatgat taaacacggc
    16201 tatctgacgc cgccagaacg actggatatg ccagtagtgc aatacgattt cagccgcttg
    16261 caggcacaga gtaacgggct gttcagcgaa gctgatctca accgtgagct gaaaaaacag
    16321 caacgcatta cgccacatat catcagccag atcatggagt tcgccgaaaa gcgcaaaggg
    16381 gtaatgattt tcgccgccac cgttgaacac gcaaaagaga ttgtgggatt actgcctgcc
    16441 gaagatgctg cactgattac tggcgacccc cccggcgctg agcgcgatgt gttaattgaa
    16501 gattttaaag cccagcgttt tcgctatctg gtcaacgtcg cagtactgac caccggattt
    16561 gacgccccgc acgtcgatct tatcgccatt ctgcgcccta ccgaatcagt gagtctttac
    16621 caacaaattg tcgggcgcgg tctgcgtctc gctccgggca agactgattg tttaattctt
    16681 gattatgcgg gtaatcctca cgatctctac gcgccggaag ttggtacacc gaaaggcaaa
    16741 agtgacaacg ttccggtaca ggttttctgc cctgcctgcg gttttgccaa caccttttgg
    16801 gggaaaacga ccgccgacgg gacattgatt gaacactttg gtcgccgctg tcagggatgg
    16861 tttgaagatg acgacggtca tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc
    16921 ccgcaatgta acgcagaaaa tgatattgcc gctcgccgct gccgcgaatg tgacacggta
    16981 ctggttgatc cggatgatat gttaaaagcg gcgctacgac tgaaagatgc gctggtatta
    17041 cgctgtagcg gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc
    17101 acctattacg atgaagacgg cgcggatgtg agtgagcgtt tccgtctgca aacgcccgcc
    17161 cagcgtaccg ccttcgagca gctttttatc cgcccgcata cgcgcacacc gggcatccct
    17221 ctgcgctgga tcaccgccgc cgatatcctc gcccagcaag ccttactgcg acatccggat
    17281 tttgtcgtcg cccgcatgaa aggtcagtac tggcaagtgc gtgaaaaagt gttcgattac
    17341 gaaggtcgtt ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg attgatgtat
    17401 aaggcgattg agtatagaat ctcgcccgct tttgcatacg caaagcagat cacttacctg
    17461 ttgctgggtc gcctgtagca ggattaattt aagagagaaa gaaatgttta ctatcaacgc
    17521 agaagtacgt aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt
    17581 cccggcaatc atctacggtg gcaaagaagc accgctggct atcgagctgg atcacgacaa
    17641 agtcatgaac atgcaagcta aagctgaatt ctacagcgaa gttctgacca tcgttgttga
    17701 cggtaaagaa atcaaagtta aagctcagga cgtacagcgt cacccgtaca aaccgaagct
    17761 gcagcacatc gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccg
    17821 gcggggtttt ttgtatctgc agattatgcc tgattacggt attgctattt tttgcaggcc
    17881 agataaggcg ttcacgccgc atctggcatt cagaacacaa aaccgattaa ttgccgccag
    17941 acgtccggcg ctgcaattgg tcgcgcaaat tcggcggcgt gcctttaatg gtcaaggtat
    18001 cggttgctgg atcccagaag atgcgttcac ctaacagcat cgcatcaaag ttaatagtca
    18061 agccaccgcc gctaccggca aacttcgtca actgacgcag tgtgctacgg tctgccggga
    18121 agctctcttc cagttcgtag cctttttcag ccgcaaactc cgtgaagctc acttcgctta
    18181 cgcctgccag ctctttcgac agcgattcca gttcgatctc ttcgcctgcc tgcagctgct
    18241 cattacagta gctataaacc tgctggcgca cgttctgtcg ctctgcttta tccagttgtg
    18301 cttcggcagt gaaatcatca accgcttgca acagcccacg gttttgcgct ttcgcgttca
    18361 atccttcgct ggccccgagg aaatccataa agaaatccgc cactttgcgc cctactcgcc
    18421 ctttcaggaa agtgagatag cgggtggact ctggattggt ttcccattcg gttaaatcga
    18481 tacgcgcaac aatatccgca tgattgatat caagataatg ggttgggttg atgtcgagat
    18541 tttcgttaac gcgcatactg ctcaggttgc tcagaactgc caccagcaga tactccaccg
    18601 ccagataacg gtagtggcaa aacagcacga atccaccgtc agcaaaagga tatttcgcta
    18661 attcgtcacg caggcgaccg gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct
    18721 gacgctgtaa gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag ccgtaagctt
    18781 tatttttggc gctatagacc cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg
    18841 gttccagcaa tgaatcgcgc aacaccagct caaggttctg ctcatcacgc ttgataagct
    18901 ggtgcagagc aatctggttg atatccagac tcatgataaa ctctccttta agaccgggcg
    18961 gtattcaacc accgccggtt aagcgacgca ataaaagatg gcggtcattt tacagaaggc
    19021 agcgtaacga gaatgattta acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat
    19081 atgttgccct ttcatcaact aactgatttc gatttatgcc acaaatttcc cgctacagta
    19141 atgaacaggt tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa cataaggctc
    19201 cgaccgacct ttccctgatg gtgttaggca acatggtcac taaccttatc aacaccagca
    19261 ttgccccggc ccaacgccag gcaattgcca actcttttgc ccgcgcctta cagtcctcta
    19321 tcaacgaaga caaagcgcac taagggaaac agataacagg ttatggtaac tcatcgtcag
    19381 cgctaccgtg aaaaagtctc ccagatggtc agttgggggc actggtttgc actgttcaat
    19441 attctgcttt cgctcgtcat tggcagccgt tacctgttta tcgccgactg gccgacaacg
    19501 cttgctggtc gcatttattc ctacgtaagc attatcggcc atttcagctt cctggtgttc
    19561 gccacctact tgctgatcct cttcccgctg acctttatcg tcggctccca gaggctgatg
    19621 aggtttttgt ccgtcattct ggcgacggcg ggaatgacgc tattactgat cgatagcgaa
    19681 gtctttactc gtttccatct ccatcttaat cccatcgtct ggcagttggt tatcaaccca
    19741 gacgaaaatg agatggcgcg cgactggcag ctgatgttca tcagcgtgcc ggttatttta
    19801 ttgcttgaac tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt
    19861 cgacgcttcg cgcgcccgct ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg
    19921 gtgtatatct gggccgatgc caacttctat cgcccgatca ccatgcagcg cgctaacctg
    19981 ccgctttcgt acccgatgac ggcgcgacgt ttccttgaga agcatggcct gcttgatgcg
    20041 caggagtatc aacgccgtct tattgagcaa ggtaatccag acaccgtttc cgtacagtat
    20101 ccgttaagcg aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct gttgattact
    20161 gtcgatggcc tgaactactc acgcttcgag aagcagatgc ctgcgctggc aggttttgct
    20221 gagcaaaata tttcgttcac gcgccatatg agctccggca acactacaga caacggcatc
    20281 tttggcctgt tctatggcat ctcgccgagc tatatggacg gcattctgtc gacccgtacg
    20341 cctgcggcat taattactgc gcttaatcag caaggctatc agctggggtt attctcatca
    20401 gatggcttta ccagcccgct gtatcgccag gcattgttgt cagatttctc gatgccgagc
    20461 gtacgcaccc aatctgacga tcagaccgcc acgcagtgga tcaactggct gggccgctac
    20521 gcacaagaag ataaccgctg gttctcgtgg gtttctttca atggcactaa cattgacgac
    20581 agcaatcagc aggcatttgc acggaaatat agccgggcgg caggcaatgt cgatgaccag
    20641 atcaaacgcg tgctcaatgc actgcgtgat tctggcaaac tggacaatac ggtggtgatt
    20701 atcactgccg gtcggggtat tccactgagc gaagaggaag aaacctttga ctggtcccac
    20761 ggtcatctgc aggtgccatt agtgattcac tggccaggca cgccggcgca gcgtattaat
    20821 gctctgactg atcataccga tctgatgacg acgctgatgc aacgcctgct gcatgtcagc
    20881 acacctgcca gcgaatattc gcaaggtcag gatttgttca accctcaacg ccgtcattac
    20941 tgggttactg ctgcggataa cgatacgctg gcaattacca ccccgaaaaa gacgctggtg
    21001 ctgaacaata acggtaaata ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa
    21061 aaaccacagt taagtttgtt attgcaagtg ctgacagacg agaagcgttt tatcgctaac
    21121 tgattaatta taaatcagtt agcgaaatat cttacttgca atcggtgtgg aaaacggtag
    21181 tattagcagc cacgagtcgg cacgtagcgc agcttggtag cgcaccgtca tggggtgtcg
    21241 ggggtcggag gttcaaatcc tctcgtgccg accaaaaatc ccaagaaaaa accaaccctt
    21301 acggttggtt tttttatatc tgcaattaat tcgataaaca gaccgtgaca catcacagcc
    21361 tgtttatttt ctgttatcag aacgtccaga ccacacccgc ctgagtattc cacggcgatt
    21421 ctacccctgc gccattgcca tagctgactg acaaatgacc gcttaacgtc ggggtaaacg
    21481 atgaccttat cccagcctga taaacgccac tggttccagc cacatcatta ttaaatttgc
    21541 catcgtcatt aactttcacc tggttagaat cggcgtattc ctgacgcacg gcggctttca
    21601 gccagggttc cagcgtcgta ccgttttgca ggtccatgtg atagcttacc gccgttcccg
    21661 cttcagcgcg taatatccgg gtatttccca catccgcgcg catgccgttt gataacgtgt
    21721 agtcctgacc atctgtggta aagccggtaa aggccagata gggtctaaca ctccacaatc
    21781 cgtcaaccca acggaacccg ctctcaacat gagcacccgc gccgttacta ttgtaatcgc
    21841 caaacgctgt tgccccatta ctcatcttgc catggatggt gttggcaaaa cggtcaactt
    21901 tcaccacccc atcaacatag gcaccgttct gatgctccca accggcataa gcccccaggg
    21961 tatagctatc gatattacct tttccgccgc gatcaaaacc aatatcagaa tgagagtaac
    22021 caaagaacaa gccgcgaatt gtactgcttt cttcacggga gaaacggcta tcgataccga
    22081 gcgtcaggcc cgtcaatgtt tgctcaaaac cagctcccgc atcagtggtc acgttgttgc
    22141 gggtgttaat tgccgaactc cacatcaccg tatcgtaact aacgcctttt acgctaccaa
    22201 gacgctcacg cacggtgtcc agttctgcat caaataccag cggttgtgcg gctgccatgt
    22261 tcagcacatc ggtggttgaa ggggtaattt gcgcgcgatt ctctgcgagg ctccagctat
    22321 ggttgccatt atccagcaag gtatattcat acgtaccgat atcaacaacg cctccggcat
    22381 tgcccaacgt aaatgcagca tcaccgccgc ccgttgttac cagtgtaagg ctatctcctg
    22441 ctgccgggct ggcaccggtg tccgtcacga atattttgaa atcacctgtt gcctgaccgg
    22501 tgacgttgag ctgatcgctc tgatgattgg ccatatcggt acgcatataa aaattgccgt
    22561 ttcccgacag ggtattggtg gtcaacgtaa tgaaattttc cgcacttgcc gtcacagatg
    22621 aacgagtcac tggctcagcc atcataatca catttgcgcc ggacatcgca aggttggcta
    22681 agttgaggcg agtgttttgg ttcgtcgtag tgatatcggc aatgatggtc catgcgctat
    22741 tttcgagcga aacgtccgct ttgctggtat ccacggcacc atgcgttcta aaagaagcgc
    22801 cctcagaaac cactacctta cccaccaaca tagcgggtgc ataatttatt tgcggcttca
    22861 tgacctcaag aatgccatca ttccctctga ctgacacgtt aaccattgtg ccagcccaga
    22921 cattagcgcg gccattgttg attaccatat tttcagagat ggcttttgag gaatacgtga
    22981 tacttccatt ctggtctgat cgaccgagat catataccgc atttgcctgc actttcgtac
    23041 cagtatcctt tcccagcgat tgcatagtgg catgctcatc aaggatagtg tcaatagcct
    23101 gcgtgtcctc cataacaatg agcccggaac catcatccag ttcataattt tttgacacac
    23161 catcctttat actaaattgg cctttactgt ttgtaccact cacttccagc gcattcgttg
    23221 agacaattaa tttcccgcct gcttttttat caacgccagt tgcagtcccg ccatccataa
    23281 cctccagtaa gccgccacta tccacagtag tattataagc gaagtcattt tcttcaactc
    23341 gcaaacttcc gccgttttcc aacaacatat tattagcaat accattcttg atatcgaact
    23401 gaccgagacg gtttgttccg aatacctcca tggcccgcgt ggttgtttta atagcaccgc
    23461 ctgctttctg atctaccgca agcgcaaatc ccccctcttt aacctccagt agtcctccct
    23521 tttcgatagc ggcaaaaacg gtccccccat ctttttcaac gattaagcgc ccgcctttaa
    23581 tcgtggtatc attcgctggg ccataaacct gtagcacacc attattgagg gtagtctggt
    23641 ttgccgcgcc acccatttga acaatttgat aaccatcatt taatactgag tattctgcgg
    23701 taccattcat ttcaatgtat tgataccctc cgttaatttc agtattacta cttacaccaa
    23761 attcttttat atgctgttct ccgccaggat tgattttcgt attattggcg gtcccataaa
    23821 ccagctggat atctttttcc aggataacac catcaactgt ctcaccatac tcaactgttg
    23881 atgccaatgc tatgggtgaa tataacgctg tagccaccag aacagatatt gcggaacgac
    23941 aaagagaaac agaaccagat tgatgcattg agctttcatc ctatgaaatt aattgcagtt
    24001 aaaagcattg ggtacagaaa atacccatag ctccataccc ggagtcagtt tttaaaaact
    24061 gtttaaagaa atgcacaagt attgtgattg attttttagt tgtttttctt gatgagaagc
    24121 tgatgcaaaa ttccgtcttt ataatgaaaa tgatgccaaa gcgaacgaca aggttgtagt
    24181 tttcactaca tgtccataca taaaatgggg taacattcac gcgcctggta gcgttaccaa
    24241 cgctacgctc aaacataatg attctaataa aacctcagga gactactatg cctgaagcaa
    24301 cgccttttca ggtgatgatt gtggatgatc atccacttat gcgacgcggt gttcgtcagt
    24361 tactggagct tgatcctggc tttgaagtgg tcgccgaagc gggcgacggc gcgagcgcga
    24421 tcgatctggc gaatagactg gatatcgacg tgatcttgct ggatctcaat atgaaaggta
    24481 tgagtggcct ggataccctc aatgccttgc gcagggatgg cgttaccgcg caaattatta
    24541 tcctgaccgt atccgatgcc tccagcgatg tctttgcgct gatagacgca ggcgcagacg
    24601 gttatctgtt gaaagacagc gacccggaag tattgctgga agcgattcgt accggagcga
    24661 aaggcaacaa agtctttagc gaacgcgtca atcagtactt acgtgaacgt gaaatgtttg
    24721 gcgcggaaga agatcccttc agcgtgctga cggagcgcga gctggatgtt ctgcacgagc
    24781 tggcacaggg gctgtcaaat aaacagattg catcggtgtt gaatatttcc gagcagacag
    24841 taaaagtaca tattcgcaat ctgctgcgta aactcaatgt ccgctcacgc gtggcggcca
    24901 ccattctgtt cctgcaacaa cgcggggcac aataaaaata gcccgatgga tttatcatcg
    24961 ggctgagatt tatgacaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacgtg
    25021 gatcgatcaa tttattgaat ttacacaatt ttgtaggccg gataaggcgt tcacgccgca
    25081 tccggcataa actaagcgca ctttgtcatc agtctagccc gatggcatca ccatcgggcc
    25141 tctttttatt tactctcctg cggcgacaaa tgttgcatcg cctgcgcgat actacgttca
    25201 atcaccgcac ggcgagtatc gttggcaggt aagagtttca acatcatctc ccacgcggca
    25261 acggcttcgc caaatcgctg ctgctcaaag gcattaaacg catacatgct tagcacacgg
    25321 atattgctat ggtccgttct caccagctga cgtagcagtt caccgccgag gcggttgtcg
    25381 ttgggatcag atgaacgagt caacgcttca gcgtatccca gtgcggcatc gctgttcttc
    25441 ggatcgaggc gatacgcagt agcgtatgca tcggtggcga tactggcgtt acccagcgcc
    25501 atgccaacgc ggcccaacat aatccagcct tctatatctc ccggattttt ttgcagttga
    25561 gtacgcatcc ccagcgcaag gcgcgacatc tcttcttcgt tgagcggatc ggctttcggg
    25621 tccagcgccc tgtccagcaa cgccggagcc tgtgccgtgg cctgctgcca gattttcacc
    25681 tgctgataat tgccagtctg gtagtagctg acgccagcca caattaacgc caccacaata
    25741 cccggcagat aaacaacata tccggcacgc ttaccttccg gcacgctttg ttcaggaaac
    25801 gcttccggca ccacgcgtac ccgacgccgc gagcgggcgt atatgaccca accgccaatg
    25861 ccaatagcca ctactggcag cacccacagc agcacggtca gcggcgttaa cggcggatcg
    25921 taagtgacga agttgccgta acgcgccacc atataatcga caatctcttt tttactttta
    25981 ccttcctgca tcagttcata cactttctga cgcaggtcgg tggcaatcat cgagttggaa
    26041 tcggcaatgc tgttgttctg gcatttcggg cagcgcagtt cttcagtgag ctgacggaac
    26101 tgttgttcct gtgcttcatc tttaaactgc aacacgtcga tggtcgccag cgctgagccg
    26161 gagatcatca gcatcagcac gcccaataaa aacctcattg tgcggcctcc ttactgtatt
    26221 tctcccacag cggcttgatc tcttcttccc agacgcgagg attgagatcg cccgcatggc
    26281 gatagcgaat gataccgttg ccgtcaataa ggaaggtttc tggcgcgcca tagacaccga
    26341 gatccagccc taacatgccg tcgccatcaa acaggcttag cgcgtaagga ttgcccagct
    26401 ctttcagcca gctgattgcc ttctggcgat cgtctttata gttcatgccg accacgcgaa
    26461 tgccctgcgc tgaaagctga ttcagatatt gatgttccgc acggcaggtc ggacaccaag
    26521 tcgcccagac gttaagcagt actggtttgc cctgagtcag cacatccgcc tgataaaact
    26581 gccccggatt gtccagtgat tcgagacgaa acttcggcac aggcttgcca atgagcgccg
    26641 attccagatt ggtcggatca tccccttcgg cattacgcgc cagctgccac agcagcgccg
    26701 cggcaatcgc caggaagata atcaacggaa ttaacaatac tttgcgcttc atacggcctc
    26761 cggcgcagtt ttttgcggac tcacgcgctt acgatagcga ggatcaaaca gacacagcag
    26821 tccgcccaac gccatcatca gcccgcccgc ccaaatccag cgaacaaatg gtttgtagta
    26881 aagacgcacg gcccacgcgc cgttttccag ctcttcaccg agcgcggcgt acaggtcacg
    26941 cgtgatgccg ccgtcaattg ccgcttcggt catcatcgac ccggcagtgt tgtaataacg
    27001 tttttccgca tacagcaccg tttccggttt accctctcgc gttacgccaa tagtcgccac
    27061 accgccgcgc cagttcgggc cagtcacctc tttgacatca cggaaggtga agcgatattc
    27121 atgaatatcg acgctatcgc cggacttcat gcgcacatca cgctcaacgc tataattctg
    27181 gctaaaggca atgccaacaa ttgtcactgc cagccccagg tgagccgcca ccatccccca
    27241 ataactgaag gtggttttcg tgccgcgtga aatacgcagc gcagcttccg cagttgccag
    27301 caccgcaatc cagcaggcca ttgccaggcc gagcaccgtc atcgccacaa ctttgctttc
    27361 gaacagccac ggcaacagca gagacagcac cagcgtagag atgaaggcga taatcaataa
    27421 attgcggatt ttacgtgggc gatcgcgccc ccagcgcacc agaggaccga caccaagcag
    27481 tagcgcaaac ggcaccatca gccaggtaaa catggtgttg aagaacggtt cgccaatcga
    27541 aatactgccc agtccaagtt gcttgtgcac cagcggcagc agcgtcccca gcaacaccac
    27601 cagcatcgcg gcgaccagca aaacgttgtt cgctaacagc aaagattccc gcgaccacag
    27661 cgcattgttt acgcgtgagc gaactttgtg tccacgcgcg gcaaacagca gcagcgaacc
    27721 gccaatcacc agcaccataa aggcgaggat aaacataccg cgcgccggat cagacgcaaa
    27781 cgcgtgtacc gataccagca cgccggaacg cacgaggaaa gtccccaaca gacacaacga
    27841 gaaggcactg attgccagca gtaatgtcca cgctttgaag ctggcgcgtt gttcagtgac
    27901 cgccagtgag tgcatcagtg cagtccccac cagccacggc ataaacgagg cgttttctac
    27961 cggatcccag aaccaccagc caccccagcc gagttcgtaa taggcccatg cggaaccgag
    28021 cacaatgccg agcgtcagga aaatccacgc cgccagcgtc cacgggcgag taaaacgcgc
    28081 ataagtgctg tccagacgcc cgctcagcaa agaagcaatg gcaaaagcaa acgccaccga
    28141 gaaacccacg taccccatat aaagcagagg cggatggaag atcagccccg gatcctgtaa
    28201 cagcgggtta agatcgcgcc cttcaatcgg gaagttcggc agcgtgcgtg agaacgggtt
    28261 agaggtaaag agaatgaaca gcaaaaagcc gacgctgacc atccccatta tcgccagtac
    28321 acgggccaca atatccagcg gaatacgctg actaaaaatc gccaccgcaa aggtccagcc
    28381 gctcatcagc agcacccaca gcagaagcga accttcatgc gcgccccagg tagccgccac
    28441 gcgataccac accggaagct gggtattgga gttgctggca acataggtga cggtaaagtc
    28501 gttgaccacg aaagcattga ccagcaccag aaatgcgcca gccacagaca taaacagcag
    28561 ccaggcaaac aagcgggaag acgccatcat gcgcgcatct ccgcgcgcca cgccccatag
    28621 cggatacacg gacagcagca gcgcaatccc taacgccaag cacagcagcc cgtttccaat
    28681 ttccggcatc atgatgctgg gtccttataa acactcgccg ggcgacggtg gttagcttcc
    28741 atcgctttct caacttctgg cggcgtgtag ttttcgtcgt gtttcgccag cacttctttc
    28801 gcgaggatat gattgccttt ttccagctcg ccctgcacca caacgccctg cccttcacgg
    28861 aacagatccg gcaaaatgcc ttcgtaagag acatccactg agccttcagc atcgtaaatg
    28921 gtgaaggtca ctttcagcga attgggatcg cgctgcacgc tacccggcat caccatcccg
    28981 ccaacgcgca gacgctgacc gacttccggc atctgctgag tttcacgctt gccgtagaga
    29041 atttcccccg gcgtataaaa gagatcgata ttcgagcgca gcgcatatag caccagaccg
    29101 atagtcagcg ccagccctgc caacacggca caggcaatcc acaagcggtt tttacggcga
    29161 atattcatgc agcctcctgc tgttgcgcag cacgtaaacg cgcctcacgc gcccgctgtt
    29221 gcgccacacc acgcagaatt gcgcgatgtt gcatcaccga gtgcacgacc aaaaccacca
    29281 gcggaataac ggtcatcacc accgccagcc agacaaaaaa ggcgtaaccg cccattgcga
    29341 aaaattcatt ccaggaagca aatgcagggg tcatttacgg cctcttttca gtatcagttc
    29401 actcacccac ggacggcgtt tttccatcag caaaatcaaa ttacgcatcc gcatcagcgt
    29461 cagcgtggca gacaggagca ggaagccaaa aatcgaccag cgcagcggcg aacgcatcgc
    29521 cggatcgata ctttgctgca tccgcgttga tccctgatgc agggtgttcc accactccac
    29581 ggagtaatga ataatcggca gattcaccac gccaatcagc accaggatac ctgccgcacg
    29641 gcccgccaga cggcggtcgt cgaaggcgtg ccacagggca atcacaccca catacaaaaa
    29701 cagcagcacc agttcagaag tcagacgtgc atcccatacc caccaggtgc cccacatcgg
    29761 ttttccccat gcagagccgg taaccagggc aataaaggta aacacggcac caatgggggc
    29821 catcgccgcc accgccaggt tggccatttt catctgccag acaaggccaa taaacgccgc
    29881 caccgccatt gatgcataaa tgcccatcga ccagatcgcc gcaggcacat gcaggtagat
    29941 aatgcggtag ctatttccct gctgataatc agccggagca aagccgaatc cccagatcca
    30001 gccgacggta agcacgacca cactggcaat tgccagccac ggtataaacc agccacagat
    30061 ttgatacagc cgtggtggga tcgccagttg atgcagtgtt ttccacatag tttcgatacc
    30121 agactcgaac aaaaatcagt aatccagcgt tattgaatgc tgattcgtaa cgctgccgcc
    30181 gtcgcaaaag gacttaatgt cgcggtgcct gccagcaacg cgcctaaaat tgccagatac
    30241 ccatcaacgg gcaaatgcat agaagccgcg tccatcgcgg cggtggcaaa gatgagtaat
    30301 gggatagtca gcggtaacac cagtatgctg agcagcacac caccgcgctt aagtcccact
    30361 gtcagcgcca cgcccggtgc accgagaaag ccaagcgtag gcgttcccag cagcagcgtc
    30421 agcgccatca cttgccagcc ataaacatcc attcccagta gcattgctac cagtggcgaa
    30481 aggatgagta acggcagacc ggttaccatc cagtgcgcca tcaccttcgc cagcacaacg
    30541 gcgggcaagg gtaacggcaa caacatcaat tgttcaagac tgccgtcctg caaatcgtca
    30601 cggaacagtc gttccagcgc cagcaaggat gaaagcagcg cagcaaccca gataatgccc
    30661 ggtgcaatac gcgccagcag ttgcggctcc ggaccgatac tgagcggaaa aagggtaatt
    30721 acaatcagga agaaccacag cgggttggcg atttcggcgc tatggcgaaa cgctacgcgc
    30781 agttcaagac ggaaaatacg ccagaacatc atgcggccct cgtttgcgtc agtgaaatgc
    30841 ggcgaatttt actttcagca acgttgagcg gctggtgggt agtcagaatc acaatccccc
    30901 cctgctccgt atgctgcgcc atacgctggg tcagacgatc gacgccgtta acgtcaatcg
    30961 cggtaaaagg ctcgtcgagg atccataatg tggcacgggt caaccacaga cgcgctaaag
    31021 cgacgcggcg ttgttgcccg gcagaaagtt gatttaccgg aatatcttcg aatccggcaa
    31081 gcccggcctg cgccaaggct tccagacatt gtgcggcatc gccatcgcga tgataaaagt
    31141 gcagattttc taacgccgtc agccgggttt tgatccccgg ctgatggcct atccataaca
    31201 ggttttgatg gtagctgtcg cgtacctgat gcaagggctg cccttgccag agaacctcgc
    31261 cagcgtcagg gcgagacaac cccgtcagca aacggagaag cgttgtcttc cccgcg
//
LOCUS       DAJVQH010000036        30392 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJVQH010000036 DAJVQH010000000
VERSION     DAJVQH010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.036"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..378
                     /gene="tuf"
                     /locus_tag="OQM87_001712"
     CDS             <1..378
                     /gene="tuf"
                     /locus_tag="OQM87_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1507877.1"
                     /translation="RAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILS
                     KDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDD
                     GLRFAIREGGRTVGAGVVAKVLS"
     gene            608..991
                     /gene="secE"
                     /locus_tag="OQM87_001713"
     CDS             608..991
                     /gene="secE"
                     /locus_tag="OQM87_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCS1507878.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            993..1538
                     /gene="nusG"
                     /locus_tag="OQM87_001714"
     CDS             993..1538
                     /gene="nusG"
                     /locus_tag="OQM87_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCS1507879.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1697..2125
                     /gene="rplK"
                     /locus_tag="OQM87_001715"
     CDS             1697..2125
                     /gene="rplK"
                     /locus_tag="OQM87_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCS1507880.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2129..2833
                     /gene="rplA"
                     /locus_tag="OQM87_001716"
     CDS             2129..2833
                     /gene="rplA"
                     /locus_tag="OQM87_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCS1507881.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3246..3743
                     /gene="rplJ"
                     /locus_tag="OQM87_001717"
     CDS             3246..3743
                     /gene="rplJ"
                     /locus_tag="OQM87_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCS1507882.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3810..4175
                     /gene="rplL"
                     /locus_tag="OQM87_001718"
     CDS             3810..4175
                     /gene="rplL"
                     /locus_tag="OQM87_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCS1507883.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4495..8523
                     /gene="rpoB"
                     /locus_tag="OQM87_001719"
     CDS             4495..8523
                     /gene="rpoB"
                     /locus_tag="OQM87_001719"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCS1507884.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGELVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8600..12823
                     /gene="rpoC"
                     /locus_tag="OQM87_001720"
     CDS             8600..12823
                     /gene="rpoC"
                     /locus_tag="OQM87_001720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCS1507885.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13065..14198)
                     /gene="thiH"
                     /locus_tag="OQM87_001721"
     CDS             complement(13065..14198)
                     /gene="thiH"
                     /locus_tag="OQM87_001721"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCS1507886.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14195..14965)
                     /gene="thiG"
                     /locus_tag="OQM87_001722"
     CDS             complement(14195..14965)
                     /gene="thiG"
                     /locus_tag="OQM87_001722"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCS1507887.1"
                     /translation="MLRIVDKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14967..15167)
                     /gene="thiS"
                     /locus_tag="OQM87_001723"
     CDS             complement(14967..15167)
                     /gene="thiS"
                     /locus_tag="OQM87_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCS1507888.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15151..15906)
                     /gene="thiF"
                     /locus_tag="OQM87_001724"
     CDS             complement(15151..15906)
                     /gene="thiF"
                     /locus_tag="OQM87_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCS1507889.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTADINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15899..16534)
                     /gene="thiE"
                     /locus_tag="OQM87_001725"
     CDS             complement(15899..16534)
                     /gene="thiE"
                     /locus_tag="OQM87_001725"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCS1507890.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16534..18429)
                     /gene="thiC"
                     /locus_tag="OQM87_001726"
     CDS             complement(16534..18429)
                     /gene="thiC"
                     /locus_tag="OQM87_001726"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCS1507891.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIHRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18510..18610)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18663..19139)
                     /gene="rsd"
                     /locus_tag="OQM87_001727"
     CDS             complement(18663..19139)
                     /gene="rsd"
                     /locus_tag="OQM87_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCS1507892.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19234..20007
                     /gene="nudC"
                     /locus_tag="OQM87_001728"
     CDS             19234..20007
                     /gene="nudC"
                     /locus_tag="OQM87_001728"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCS1507893.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20047..21111
                     /gene="hemE"
                     /locus_tag="OQM87_001729"
     CDS             20047..21111
                     /gene="hemE"
                     /locus_tag="OQM87_001729"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCS1507894.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21268..21939
                     /gene="nfi"
                     /locus_tag="OQM87_001730"
     CDS             21268..21939
                     /gene="nfi"
                     /locus_tag="OQM87_001730"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCS1507895.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21982..22572
                     /locus_tag="OQM87_001731"
     CDS             21982..22572
                     /locus_tag="OQM87_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCS1507896.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22759..23031
                     /gene="hupA"
                     /locus_tag="OQM87_001732"
     CDS             22759..23031
                     /gene="hupA"
                     /locus_tag="OQM87_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCS1507897.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23044..23739
                     /locus_tag="OQM87_001733"
     CDS             23044..23739
                     /locus_tag="OQM87_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCS1507898.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRASFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23741..24166)
                     /gene="zraP"
                     /locus_tag="OQM87_001734"
     CDS             complement(23741..24166)
                     /gene="zraP"
                     /locus_tag="OQM87_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCS1507899.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24404..25780
                     /gene="zraS"
                     /locus_tag="OQM87_001735"
     CDS             24404..25780
                     /gene="zraS"
                     /locus_tag="OQM87_001735"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCS1507900.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25777..27102
                     /gene="zraR"
                     /locus_tag="OQM87_001736"
     CDS             25777..27102
                     /gene="zraR"
                     /locus_tag="OQM87_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCS1507901.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27099..28388)
                     /gene="purD"
                     /locus_tag="OQM87_001737"
     CDS             complement(27099..28388)
                     /gene="purD"
                     /locus_tag="OQM87_001737"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCS1507902.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28400..29989)
                     /gene="purH"
                     /locus_tag="OQM87_001738"
     CDS             complement(28400..29989)
                     /gene="purH"
                     /locus_tag="OQM87_001738"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCS1507903.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7120 a         7917 c         8364 g         6991 t
ORIGIN      
        1 cgtgctggtg agaacgtagg tgttctgctg cgtggtatca aacgtgaaga aatcgaacgt
       61 ggtcaggtac tggctaagcc gggcaccatc aagccgcaca ccaagttcga atctgaagtg
      121 tacattctgt ccaaagatga aggcggtcgt catactccgt tcttcaaagg ctaccgtccg
      181 cagttctact tccgtactac tgacgtgact ggtaccatcg aactgccgga aggcgtagag
      241 atggtaatgc cgggcgacaa catcaaaatg gttgttaccc tgatccaccc gatcgcgatg
      301 gacgacggtc tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgta
      361 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
      421 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      481 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      541 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      601 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      661 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      721 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      781 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      841 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      901 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      961 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
     1021 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
     1081 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
     1141 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
     1201 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
     1261 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
     1321 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1381 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1441 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1501 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1561 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1621 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1681 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1741 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1801 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1861 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1921 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1981 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     2041 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     2101 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     2161 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     2221 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     2281 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     2341 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2401 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2461 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2521 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2581 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2641 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2701 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2761 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2821 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2881 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2941 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     3001 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     3061 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     3121 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     3181 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     3241 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     3301 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3361 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3421 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3481 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3541 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3601 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3661 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3721 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3781 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3841 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3901 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3961 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     4021 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     4081 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     4141 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     4201 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     4261 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     4321 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4381 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4441 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4501 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4561 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4621 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4681 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4741 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4801 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4861 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4921 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4981 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     5041 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     5101 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     5161 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     5221 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     5281 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     5341 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5401 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5461 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5521 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5581 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5641 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5701 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5761 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5821 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5881 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5941 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     6001 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     6061 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     6121 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     6181 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     6241 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     6301 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6361 ctggatgaag gaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6421 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6481 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6541 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6601 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6661 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6721 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6781 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac tggttgaacg tggcgacgtg
     6841 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6901 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6961 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     7021 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     7081 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     7141 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     7201 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     7261 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     7321 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7381 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7441 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7501 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7561 cacgagttcg agaagaaact cgaagcgaaa cgccgaaaaa tcacccaggg cgacgatctg
     7621 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7681 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7741 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7801 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7861 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7921 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7981 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     8041 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     8101 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     8161 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     8221 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     8281 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     8341 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8401 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8461 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8521 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8581 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8641 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8701 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8761 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8821 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8881 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8941 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     9001 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     9061 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     9121 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     9181 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaactaagcg
     9241 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     9301 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9361 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9421 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9481 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9541 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9601 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9661 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9721 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9781 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9841 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9901 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9961 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
    10021 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
    10081 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
    10141 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
    10201 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
    10261 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
    10321 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10381 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10441 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10501 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10561 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10621 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10681 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10741 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10801 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10861 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10921 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10981 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    11041 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    11101 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    11161 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    11221 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    11281 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    11341 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11401 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11461 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11521 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11581 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11641 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11701 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11761 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11821 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11881 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11941 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    12001 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagaaccggc
    12061 aatcttggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    12121 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    12181 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    12241 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    12301 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12361 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12421 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12481 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12541 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12601 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12661 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12721 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12781 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12841 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12901 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12961 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    13021 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    13081 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    13141 gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    13201 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    13261 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    13321 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13381 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13441 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13501 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13561 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13621 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13681 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13741 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13801 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13861 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13921 ccgcagtacg tgcagttgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13981 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    14041 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    14101 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    14161 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    14221 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    14281 gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    14341 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgcggcat
    14401 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14461 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14521 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14581 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14641 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14701 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14761 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14821 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14881 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14941 acgttttgtc cacaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    15001 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    15061 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    15121 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    15181 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    15241 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    15301 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15361 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15421 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15481 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15541 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15601 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15661 cgggctgttg atatctgcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15721 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15781 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15841 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15901 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15961 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    16021 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    16081 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    16141 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    16201 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    16261 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    16321 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16381 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16441 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16501 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16561 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16621 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16681 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16741 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16801 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16861 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16921 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16981 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    17041 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggtg
    17101 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    17161 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    17221 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    17281 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    17341 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17401 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17461 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17521 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17581 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17641 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17701 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17761 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17821 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17881 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17941 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    18001 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    18061 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    18121 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    18181 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    18241 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    18301 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18361 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18421 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18481 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18541 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18601 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18661 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18721 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18781 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18841 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18901 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18961 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    19021 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    19081 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    19141 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    19201 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    19261 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    19321 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19381 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19441 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19501 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19561 accgaatggg cgatgctgtg cagccattgc cgtgagcgtt actacccgca aatcgccccc
    19621 tgcattattg ttgccatccg tcgcgatgat tcgatcctcc tcgcccagca tacccgccat
    19681 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19741 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19801 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19861 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19921 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19981 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    20041 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    20101 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    20161 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    20221 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    20281 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    20341 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20401 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20461 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20521 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20581 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20641 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20701 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20761 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20821 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20881 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20941 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    21001 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    21061 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    21121 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    21181 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    21241 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    21301 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21361 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21421 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21481 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21541 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21601 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21661 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21721 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21781 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21841 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21901 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21961 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    22021 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    22081 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    22141 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    22201 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    22261 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    22321 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22381 caggctggtc gcgaaatgag cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22441 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22501 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22561 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22621 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22681 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22741 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22801 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22861 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22921 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22981 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    23041 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    23101 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    23161 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    23221 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    23281 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    23341 ccgccggtga acattcgcgc gagttttgac gatcgcggcg atctgagctt tatgcaacgt
    23401 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23461 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23521 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23581 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23641 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23701 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23761 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23821 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23881 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23941 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    24001 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    24061 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    24121 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    24181 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    24241 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    24301 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24361 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24421 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24481 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24541 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24601 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24661 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24721 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24781 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24841 ttatcgccag tttcaaccga tgtttgctgc tggaatgcac cggatgcgcc atatgcagca
    24901 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24961 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    25021 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    25081 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    25141 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    25201 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    25261 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    25321 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25381 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25441 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25501 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25561 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25621 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25681 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25741 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25801 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25861 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25921 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25981 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    26041 gtcgagacgg cggtagaggc actgaaaact ggggcgctgg attatctcat caagccgctg
    26101 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    26161 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    26221 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    26281 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    26341 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26401 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26461 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26521 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26581 cagactatct cggttgatgt ccggctgatt gcggcgactc atcgcgatct tgccgcagag
    26641 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26701 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26761 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26821 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26881 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26941 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    27001 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    27061 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    27121 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    27181 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    27241 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    27301 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27361 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27421 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27481 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27541 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27601 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27661 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27721 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27781 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27841 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27901 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27961 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    28021 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    28081 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    28141 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    28201 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    28261 agcgcgggtt ccagcgcagt gcctgcatta cccggagcaa caaaaacagt ctcaaccagc
    28321 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28381 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28441 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28501 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28561 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28621 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28681 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28741 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28801 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28861 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28921 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28981 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    29041 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    29101 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    29161 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    29221 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    29281 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    29341 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29401 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29461 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29521 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29581 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29641 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29701 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29761 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29821 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29881 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29941 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatccct
    30001 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    30061 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    30121 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    30181 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    30241 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    30301 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30361 ttcggttgaa gagaaaaatc ctgaaattca gg
//
LOCUS       DAJVQH010000037        29934 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJVQH010000037 DAJVQH010000000
VERSION     DAJVQH010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.037"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            229..1677
                     /gene="uacT"
                     /locus_tag="OQM87_001739"
     CDS             229..1677
                     /gene="uacT"
                     /locus_tag="OQM87_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCS1507904.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1679..1804
                     /locus_tag="OQM87_001740"
     CDS             1679..1804
                     /locus_tag="OQM87_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507905.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1801..1872)
                     /locus_tag="OQM87_001741"
     CDS             complement(1801..1872)
                     /locus_tag="OQM87_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507906.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            1927..2475
                     /gene="idi"
                     /locus_tag="OQM87_001742"
     CDS             1927..2475
                     /gene="idi"
                     /locus_tag="OQM87_001742"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCS1507907.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            complement(2518..4035)
                     /gene="lysS"
                     /locus_tag="OQM87_001743"
     CDS             complement(2518..4035)
                     /gene="lysS"
                     /locus_tag="OQM87_001743"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS1507908.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4045..5143)
                     /gene="prfB"
                     /locus_tag="OQM87_001744"
     CDS             complement(join(4045..5067,5069..5143))
                     /gene="prfB"
                     /locus_tag="OQM87_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCS1507909.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5234..6967)
                     /gene="recJ"
                     /locus_tag="OQM87_001745"
     CDS             complement(5234..6967)
                     /gene="recJ"
                     /locus_tag="OQM87_001745"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCS1507910.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(6973..7683)
                     /gene="dsbC"
                     /locus_tag="OQM87_001746"
     CDS             complement(6973..7683)
                     /gene="dsbC"
                     /locus_tag="OQM87_001746"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCS1507911.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            complement(7708..8604)
                     /gene="xerD"
                     /locus_tag="OQM87_001747"
     CDS             complement(7708..8604)
                     /gene="xerD"
                     /locus_tag="OQM87_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCS1507912.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8716..9237
                     /gene="fldB"
                     /locus_tag="OQM87_001748"
     CDS             8716..9237
                     /gene="fldB"
                     /locus_tag="OQM87_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCS1507913.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9277..9684)
                     /locus_tag="OQM87_001749"
     CDS             complement(9277..9684)
                     /locus_tag="OQM87_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCS1507914.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9665..9931)
                     /gene="sdhE"
                     /locus_tag="OQM87_001750"
     CDS             complement(9665..9931)
                     /gene="sdhE"
                     /locus_tag="OQM87_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCS1507915.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            10174..11154
                     /gene="ygfZ"
                     /locus_tag="OQM87_001751"
     CDS             10174..11154
                     /gene="ygfZ"
                     /locus_tag="OQM87_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCS1507916.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11350..12009)
                     /locus_tag="OQM87_001752"
     CDS             complement(11350..12009)
                     /locus_tag="OQM87_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCS1507917.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12173..12484)
                     /locus_tag="OQM87_001753"
     CDS             complement(12173..12484)
                     /locus_tag="OQM87_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCS1507918.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            12529..13961
                     /gene="bglA"
                     /locus_tag="OQM87_001754"
                     /pseudo
     CDS             12529..13961
                     /gene="bglA"
                     /locus_tag="OQM87_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
     gene            complement(14018..14761)
                     /locus_tag="OQM87_001755"
     CDS             complement(14018..14761)
                     /locus_tag="OQM87_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1507919.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15028..17901)
                     /gene="gcvP"
                     /locus_tag="OQM87_001756"
     CDS             complement(15028..17901)
                     /gene="gcvP"
                     /locus_tag="OQM87_001756"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCS1507920.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18020..18409)
                     /gene="gcvH"
                     /locus_tag="OQM87_001757"
     CDS             complement(18020..18409)
                     /gene="gcvH"
                     /locus_tag="OQM87_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCS1507921.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18433..19527)
                     /gene="gcvT"
                     /locus_tag="OQM87_001758"
     CDS             complement(18433..19527)
                     /gene="gcvT"
                     /locus_tag="OQM87_001758"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCS1507922.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLIVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            19818..19901
                     /locus_tag="OQM87_001759"
     CDS             19818..19901
                     /locus_tag="OQM87_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507923.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            complement(19975..21177)
                     /gene="ubiI"
                     /locus_tag="OQM87_001760"
                     /gene_synonym="visC"
     CDS             complement(19975..21177)
                     /gene="ubiI"
                     /locus_tag="OQM87_001760"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCS1507924.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(21200..22378)
                     /gene="ubiH"
                     /locus_tag="OQM87_001761"
                     /gene_synonym="visB"
     CDS             complement(21200..22378)
                     /gene="ubiH"
                     /locus_tag="OQM87_001761"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCS1507925.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22375..23700)
                     /gene="pepP"
                     /locus_tag="OQM87_001762"
     CDS             complement(22375..23700)
                     /gene="pepP"
                     /locus_tag="OQM87_001762"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCS1507926.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23726..24304)
                     /gene="ygfB"
                     /locus_tag="OQM87_001763"
     CDS             complement(23726..24304)
                     /gene="ygfB"
                     /locus_tag="OQM87_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCS1507927.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24472..24801
                     /gene="zapA"
                     /locus_tag="OQM87_001764"
     CDS             24472..24801
                     /gene="zapA"
                     /locus_tag="OQM87_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCS1507928.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24843..25026
                     /gene="ssrS"
                     /locus_tag="OQM87_001765"
     ncRNA           24843..25026
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OQM87_001765"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25047..25649
                     /locus_tag="OQM87_001766"
     CDS             25047..25649
                     /locus_tag="OQM87_001766"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCS1507929.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25746..25805)
                     /locus_tag="OQM87_001767"
     CDS             complement(25746..25805)
                     /locus_tag="OQM87_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1507930.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26138..26197)
                     /gene="ibsC"
                     /locus_tag="OQM87_001768"
     CDS             complement(26138..26197)
                     /gene="ibsC"
                     /locus_tag="OQM87_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCS1507931.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26426..27658)
                     /gene="serA"
                     /locus_tag="OQM87_001769"
     CDS             complement(26426..27658)
                     /gene="serA"
                     /locus_tag="OQM87_001769"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS1507932.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(27914..28573)
                     /gene="rpiA"
                     /locus_tag="OQM87_001770"
     CDS             complement(27914..28573)
                     /gene="rpiA"
                     /locus_tag="OQM87_001770"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCS1507933.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            28956..29849
                     /gene="argP"
                     /locus_tag="OQM87_001771"
     CDS             28956..29849
                     /gene="argP"
                     /locus_tag="OQM87_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCS1507934.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
BASE COUNT         7113 a         8063 c         7504 g         7254 t
ORIGIN      
        1 atgccagaat gattaatgaa atatcaatat gagaaaatta cagataattc cattgtcagg
       61 ttctcttgtc attttcgtgg tttaagcacg attatgtgat tgcctccgca attcctgtct
      121 ctaactcccc ttcctcgcaa aaactggcac tccacgagca tgtgtttaga cagtttcatt
      181 aacgtaaacg gttgcttttt attctggcgg gcgaaaggag aaacactgat gagcgccata
      241 gattcccaac ttccctcatc ttctgggcaa gaccgcccaa ctgatgaggt tgaccgcata
      301 ttatcaccag gaaagctgat catactcggt ctgcaacacg tccttgtcat gtacgcaggt
      361 gcagtcgctg ttcctcttat gattggtgac cgactgggcc tctcaaaaga tgctattgcg
      421 atgctcatta gctcggatct cttttgctgc gggatcgtca cattattgca atgtatcggt
      481 atcggccgct ttatggggat ccgcctgccg gtgattatgt cggtgacctt tgctgctgta
      541 acaccaatga tagccattgg gatgaacccg gatatcggcc tgctggggat atttggtgcc
      601 actatcgccg cgggttttat caccacatta ttagcgccac ttattggtcg cttgatgcct
      661 ttattcccgc cactggttac cggtgtggtt attacttcta ttgggcttag catcattcag
      721 gtgggtattg actgggccgc tggaggtaaa gggaatccgc aatatggtaa tcccgtttat
      781 ttaggtatct cctttgccgt cttaattttt atcttgctca ttactcgcta tgcgaaagga
      841 tttatgtcca acgtcgccgt attactgggg attgtatttg gctttttact ttcgtggatg
      901 atgaatgaag tcaatttatc cgggctacat gatgcctcgt ggtttgcgat tgtcaccccg
      961 atgtcgtttg gtatgccgat tttcgatccc gtttccattc tgaccatgac tgccgtgtta
     1021 atcatcgtgt ttatcgagtc gatggggatg ttcctggcac tgggtgaaat agtcggtcgt
     1081 aaactctctt cacacgatat tattcgcggg ctgcgtgtcg atggcgtagg gacaatgata
     1141 ggcggcacgt ttaacagctt cccccacacg tcattttctc aaaacgttgg cctggttagc
     1201 gtgatgcgcg ttcatagccg ctgggtgtgt atttcttcgg gaattatatt aatcctgttt
     1261 ggcatggtgc caaaaatggc ggtgctggta gcctccattc cgcaatttgt gctgggcggc
     1321 gctggtctag tgatgttcgg catggtactg gcgacaggga ttcgaattct gtcgcgctgt
     1381 aactacacca ccaaccgtta caacctctat attgtggcga tcagtctcgg cgttggcatg
     1441 actccgacgc tctctcacga tttcttttct aagttaccgg ccgtactgca accgctgcta
     1501 catagcggca ttatgctcgc aacccttagc gccgttgtgc tgaacgtctt ctttaatggc
     1561 tatcagcatc atgctgacct ggtgaaggaa tccgtctctg ataaagattt aaaagtcagg
     1621 acagtacgta tgtggcttct gatgcgcaag ctgaagaaaa atgagcatgg agaataatat
     1681 gaatttttta atgcgcgcta tattcagtct gttgttgctt tttactctct ctattcctgt
     1741 catttctgac tgtgttgcaa tggccattga aagtcgcttc aaatatatga tgctactttt
     1801 ttaaatggtt tttacctgtc ggcatccgct caaaacgggc ggttgtcgat aaacgctctc
     1861 ttggttaatc atttcactct tcaattatct ataatgatga gtgatcagaa ttacatgtga
     1921 gaaattatgc aaacggaaca cgtcatttta ttgaatgcac agggagttcc cacgggtacg
     1981 ctggaaaagt atgccgcaca cacggcagac accctcttac atctcgcgtt ctccagttgg
     2041 ctgtttaatg ccaaggggca attattagtt acccgccgcg cactgagcaa aaaagcgtgg
     2101 cctggcgtgt ggactaactc ggtttgtggg cacccacaac tgggagaaag cagcgaagac
     2161 gcggtgatcc gccgttgccg ttatgagctt ggcgtggaaa ttacgcctcc tgaatctatc
     2221 tatcctgact ttcgctaccg cgccaccgat ccgagtggca ttgtggaaaa tgaagtgtgt
     2281 ccggtatttg ccgcacgcac caccagtgcg ttacagatca acgatgatga agtgatggat
     2341 tatcaatggt gtgatttagc tgatgtatta cgcggtattg atgccacgcc gtgggcgttc
     2401 agtccgtgga tggtgatgca ggcgacaaat cgcgaagcca gaatacgatt atctgcattt
     2461 acccagctta aataaaaaac cccgacattt gccggggttg tgagcataac gtaatgctta
     2521 tttcaccgga cgcatcgccg ggaacagaat aacgtcgcgg atggtatggc tgttggtgaa
     2581 cagcattacc atacggtcga tgccaatccc cagacctgct gtcggcggta agccatgttc
     2641 cagtgcggta acgtaatctt catcgtagaa catcgcttcg tcgtcacctg cgtctttcgc
     2701 ggcaacctga tccaggaagc gttgcgcctg atcttccgcg tcattcagct cgctaaagcc
     2761 gttaccgatt tcacgaccac cgatgaagaa ctcaaagcgg tctgtgattt ccgggttaat
     2821 gtcgttacga cgcgccagcg gagaaacttc tgccggatat tcagtaatga aggtcggctg
     2881 aatcagatgt gcttctgcca cttcttcgaa gatctcggta acgatacggc ccagacccca
     2941 gctcttctca acgtggatac cgatagattc agcaatcgct ttcgcagagt cgaagttatc
     3001 cagatccgcc atgtcggttt ccgggcgata tttcttgatc gcttcacgca tggtcagttt
     3061 ttcgaacggt ttaccgaagt ccagcgtcac gtcgccgtag gtcacttccg tcttaccgag
     3121 aatatcctgt gccagagtac ggaacagcga ttcggtcagc tcgatcagat ctttgtaatc
     3181 tgcgtaagcc atgtagagtt ccatcatggt gaactctggg ttatgacgta cggaaatacc
     3241 ttcgttacgg aagttacggt tgatttcgaa tacacgctcg aagccaccga ccaccagacg
     3301 cttgaggtac agttccggcg cgatacgcag gtacatgtcg agatccagcg cgttatggtg
     3361 ggtgataaac ggacgcgcag cggcaccgcc tgggatcacc tgcatcatcg gcgtttcaac
     3421 ttccataaag ccacggttca ccatgaactg gcgaatacca gagaggatct gcgagcgtac
     3481 tttaaaggtg ttacgggatt catcgttaga gatgagatcc agataacgct gacgatagcg
     3541 cgcttcctga tcctgcaagc cgtggaattt atccggcagc ggacgcagtg ctttggtcag
     3601 cagacgcagc tcggtacagt ggatagacag ttcgccggtt ttggttttga acagtttacc
     3661 tttcgcgccg aggatgtcgc cgaggtccca ttttttgaac tgctcgttgt aaacgccttc
     3721 cggcagatcg tcacgggcaa cgtacagctg aatgcggcca cccacatcct gcagggtaac
     3781 gaaagacgct ttacccataa tacggcgggt catcatgcgg ccagcaacgg cgacttcgat
     3841 gttcagcgct tccagttctt cgttctcttt accgtcgaat tctgcgtgca attggtcaga
     3901 ggtatgatcg cgacggaaat cgttcgggaa ggcaattccc tgctcacgca ggttcgccag
     3961 cttctcacga cgcgttttca gttcattgtt aagatcgact accgcgtcag cgccctgtgc
     4021 gtgttgttca gacatgttgg ttcctcataa ccctgctttc aaacttgctt cgataaattg
     4081 atccaggctg ccgtccagca cggcctgcgt gttgcgagtt tctaccccgg tgcgcagatc
     4141 tttaatgcgg gagtcatcaa ggacataaga acgaatctgg ctgccccagc cgatgtcaga
     4201 tttgttatct tccatcgcct gtttctcggc atttttcttc tgcatctcca gttcataaag
     4261 cttcgctttc atctgcttca tggcctgatc tttgttcttg tgctgggaac ggtcgttctg
     4321 gcactgggtc acgatcccgg tcgggatgtg ggtaatacgc accgcagatt cggtacggtt
     4381 aacgtgctga ccgcccgcgc cggacgcgcg ataaacgtca atgcgcagat ccgccgggtt
     4441 gatttcgata tcaatatcat catcaacttc cggataaaca aacgcagagc tgaacgacgt
     4501 gtggcgacga ccgccggagt caaacgggct tttacgcacc aggcggtgaa cgcccgtttc
     4561 tgtacgcagc cagccgtaag cgtaatcgcc ggagattttg atcgtcacgg atttaatacc
     4621 cgccacttca ccttccgact cttcgatgat ttcagttttg aaaccacgcg attctgccca
     4681 gcgcagatac atacgctcaa gcatgctcgc ccagtcctgt gcttccgtac cgccagaccc
     4741 cgcctgaata tcgaggtaac agtcggcgct gtcatattcg ccagagaaca tacggcggaa
     4801 ctcaagctgc gccagttttt cttccagggc gtcgagttca gcaacggctt cgttaaaggt
     4861 ttcttcgtcg tcagcttcta cagccagttc cagcagacca gaaacatctt ccagcccctg
     4921 tttcatttgg tcgagggtgt cgacaacggc ttcgagggag gaacgctctt tacccagcgc
     4981 ctgtgcgcgt tcgggttcgt tccagacatc cggctgttcc agctcggcgt ttacttcttc
     5041 cagacgctct ttcttggcgt cgtagtcaaa gataccccct aagaacgtcg gagcgttccg
     5101 tgaggtcctg aatgcgatta tttaccggat taatttcaaa catggtctga tttcttttat
     5161 tgagctagtc aaaatgcggt gataagagcg ggattgtacc caatccacgc tcttttttat
     5221 agagaagatg acgctaaatt ggccagatat tgtcgatgat aatttgcagg ctgcggttgc
     5281 cgcgaaactc gttgatatcg agcttataag ccagttgcac ttcgcggacg ccgttatccg
     5341 gccagagagc ggtatcgaca ttaaaagcaa taccatccag cagtggaccg ccgccgaccg
     5401 gttcgaccat caccttcaaa tgacgttcgc ccaccagccg ctgttgcagc agacggaaat
     5461 gaccgtcaaa cagcggctcc gggaacatct gcccccacgg gccagcatcg cgcagcagct
     5521 gcgccacttc catggtcatt tcggccgggc ttaacggacc gtctgacacc acttcgcctt
     5581 gcaatagcga aggggccagc cactcagtaa ccagttcgcc aaaccgttgt tgaaagagtt
     5641 cgaatttatc ctcttccagc gacaaacccg ccgccatcgc atgaccgcca aacttgagca
     5701 tcatgccggg gtagagtgtg tctaatcgct ccagcgcatc acgcatatgc agcccctgaa
     5761 tggagcgacc ggaacctttc agcgtaccgt cacctgctgg cgcaaaggcg ataaccggac
     5821 ggtgaaaacg ctctttgata cgcgaagcca gaataccgac aacgccctga tgccattcgg
     5881 ggtgatacat tgccagcccg ccgggtagcg tgtcacggct gcgctccagt ttctcgcaca
     5941 gggtcagggc ttcaacctgc attccttgtt cgatctcttt tcgcgtctgg tttagcgcat
     6001 cgagttcatt tgccagcacg cgcgcttcgc cgatgttgtc gcacagcaac agcgccacac
     6061 caacggacat atcatccagt cgcccggcag cattgagacg cggtcccagc gcaaaaccta
     6121 aatcgctggc ggctagtttt tgtgcatcgc gatttgccac ttccagcagc gctttaatcc
     6181 ctggacggca ctttccggca cggatgcgac tcatcccctg ccaggtcaga atgcgattat
     6241 tagcgtccag cggcacgacg tccgctactg tccccagcgc taccagatcc agcagttctg
     6301 ccaggttagg aattgcgatg ccgcgctcat caaaccagcc ctgatcgcgc aaaaaggtgc
     6361 gcagcgccag catcagataa aacgccacac ccacgcctgc cagtgatttc gacgggaaat
     6421 tacagtcgcg caagttaggg ttaatgatcg cttccgctgc gggtaatgtg tcgcccggca
     6481 aatggtgatc ggtaacaata accgggatgc ccaacgagcg agcgtgctca actcccgcat
     6541 gggaggaaat accgttatcc accgtgacaa ttaactgcgc gccacgggca tgggcctgat
     6601 cgaccacttc cgggcttaag ccgtaaccgt cttcgaaacg gtttggtacc aggtagtcga
     6661 tattgctgca accaagcgag cgcatcgcca gcacgcttag agccgtgctg gtcgcgccgt
     6721 cggcgtcgaa atcaccgacc acaataatcc gcgttccttc gcgaaaagcg ttgtaaagga
     6781 tctcaacggc cttttcgaca ccgcttagtt gctgccaggg caacatacct ttaacactgc
     6841 gttccagttc ttgcgcactg cgtactcccc ggctggcgta taaacggcgc agcaagggag
     6901 gcaattcagc gggcaagtct gccgtttcat cgacttcacg gcgacgaagt tgtatctgtt
     6961 gtttcacgcg aattatttac cgctggtcat tttttggtgt tcgtcgagga attctttcat
     7021 gtctttcggc ggctggtaac ccggaacaag tgtgccattg ctcagcacaa ctgccggagt
     7081 accgctaacg ccaagctgga cgccaagtgc gtaatggtcg gcaatatcca cgtcgcagct
     7141 ggctggtgcg acgcttttac ctgccatcac atcatcaaac gctttgtttt tatctttcgc
     7201 acaccagata gctttcattt ctttctctgc atcgctgtcc agcccctggc gcgggaaagc
     7261 aagataacgc acggtgatcc ccagcgcgtt atagtctgcc atttgctcat gcagtttgtg
     7321 gcagtaacca caggtaatat cagtaaacac ggtgatgacg tgtttttcct gcggcgcttt
     7381 ataaacgatc atctcttttt caagcgcatt caactgcttt aacagcatct tattggtgac
     7441 attgaccgga gccgtgccac taacgtcata cattggcccc tgaatgatat gtttaccatc
     7501 atcggtgatg tacaacacac cgctgttagt cagaactgtc ttcatgccag ctacgggcgc
     7561 gggctgaata tcgctgcttt tgatgcccat tttggctaac gtttgttgaa ttgccgcgtc
     7621 atcagcctga gcaaagcctg aaaacgccgc taacaaagta aacaacataa aacctttctt
     7681 cataaatctt cccgttcttt tcagacatca cgcccgcggg tgatgctgtt gatgaagttg
     7741 ccgcagacgc tcggtagcga catgcgtata aatttgcgtg gtggaaagat cgctgtggcc
     7801 cagtagcatc tgcaccacgc gtaaatccgc accatgattt aataaatgag tggcaaaagc
     7861 gtgacgcaac acatgcggtg acagcttttc gctgtcgata cccgccagca cggcataatg
     7921 tttaatgcgg tgccagaagg tctgtcgcgt catctgctgc gcacgctggc tgggaaacaa
     7981 cacgtcaatt gacacaccat tcagcagcca cggacgccca tgttccagat aggtttccag
     8041 ccagtaaacc gcctcttccc ctaacggcac caggcgctct ttgttgcctt taccaatgac
     8101 ccgcactacg ccctgacgca ggctgatatc actcattgtc agcccgacca gttcagagac
     8161 acgcagcccg gtagcataca acacttcaag catggcttta tcgcgtagct ccagtggctg
     8221 atcaattaat ggcgcctgta ataaacgttc gacctgcgct tcgcttaaat cttttggcaa
     8281 acgctggggc aatttcggtg aagcgagatg cgcactggga tcgtcttcac gaaacttttc
     8341 gcgataaaga tactggaaca atcggcgcac tgcactcagc aaacgcgctg agctggtggc
     8401 tttatacccg ccctccagcc gttctgccag taatgcctgc aaatcgtcac tttgcgccgt
     8461 cgccagcgtc aacccgcggt gatgcaacca ctccaccatc attgacagat cgcgacggta
     8521 agcgttcaac gtattttcag ccagattttt ttccagccac agagcatcaa gaaactgctc
     8581 gatgcgtgcc agatcctgtt tcacttgcgc cccttatggt cactcatttg atccattatg
     8641 ccttattgtg ccgtgactaa agcgattctg atacactagc tgcaaaagcc acagcagaat
     8701 cgagaagctt acgttatgaa tatgggtctt ttttacggtt ccagcacctg ttacaccgaa
     8761 atggcggcag aaaaaatccg cgatattatc ggcccagaac tggtgacctt acataacctc
     8821 aaggacgact ccccgaaatt aatggagcag tacgatgtgc tcattctggg tatcccgacc
     8881 tgggattttg gtgaaatcca agaagactgg gaagccgtct gggatcagct cgacgacctg
     8941 aaccttgaag gtaaaattat tgcgctgtat gggcttggcg atcaactggg atacggcgag
     9001 tggttcctcg atgcgctcgg tatgctgcat gacaaactct cgaccaaagg cgtgaagttc
     9061 gtcggctact ggccaacgga aggatatgaa tttaccagcc cgaaaccggt gattgctgac
     9121 gggcaactgt tcgtgggtct ggcgctggat gaaactaacc agtatgacct tagcgacgag
     9181 cgtattcaga gctggtgcga gcaaatcctc aacgaaatgg cagagcatta cgcctgatct
     9241 cactgacggc ttagcgcata tgctttgccg tcatctttat ctttgcgtct cttgttgcaa
     9301 caaaatccgc cgtaaatccc gccattcggc ttcgtccatg ctgtcggctg ccagccataa
     9361 atgttgccgt ttaccgccat cagaacgtaa acgcagcatc atgccgctct taatcatcca
     9421 cggtgctttg acgatgctcc actcctgccc ttgccaacgc aaacgcccgt ccatcaacaa
     9481 gcgaatttcc ccctggcgag cattaataca ccgctggctg cgaacgcaat caaataccac
     9541 cagcgaaagt aacaccatcc ataacggggt gtaactgagc ggccagggca tgagtaaaat
     9601 aacagcggca accagcccat gaatcagcaa ggaaagccac tgtgcgcgcc aggagacgcg
     9661 caaatcagat tgccacagga ccacgttccc ggttccgtgt ctggatgagt cggaccatta
     9721 tttccagttc tgcatcggcc ggtttaccgt gattcatcag ccagttaaac aggtccggat
     9781 cgtcacattc cagcagacga ataaagatgc gtttttcgtc atcgcttaag ctgtcgtact
     9841 catgttcgaa aaacggcatg attgaaatat cgagttcgcg cataccacgg cggcatgccc
     9901 aatgaatgcg ggctttgttg ttaatgtcca tcttcttcct gtctcacgaa aatccagtac
     9961 ccggctattg taacgtgttt ttcgacttct tttacgggaa tatcagtaaa caccatcgcg
    10021 atcgcgaaat taattcacaa caattcaagg gcttcatttt tttggcagtc gcctcgcaga
    10081 aggcacagat cgcgtagtga aagcacttgc attgcctcat agctctttta ccattagtca
    10141 ttaatacgcc gttaagcaac tcaggactct attatggctt ttacaccttt tcctccacgt
    10201 cagcctacgg cttctgcccg tttaccgctg acgctgatga cgcttgatga ctgggcgctt
    10261 gccaccatta ctggcgcgga cagcgaaaaa tatatgcagg gtcaggtgac agcagatgtc
    10321 agccagatgg cagaagatca gcacctgctc gccgcccatt gcgacgccaa aggtaaaatg
    10381 tggagcaatt tacgtctgtt ccgcgacggc gatggctttg catggattga acggcgcagc
    10441 gtgcgtgaac cgcagctgac tgaactgaaa aaatatgcgg tattctctaa agtgaccatc
    10501 gcgccagacg acgagcgtgt gctgcttggt gttgccggtt ttcaggcgcg cgccgcgctg
    10561 gcaaatctct ttagcgaact gccttcgaaa gaaaaacagg tagtcaaaga aggcgcgacc
    10621 actttgctat ggtttgaaca cccggcagaa cgtttcctga tcgtaaccga tgaagctact
    10681 gctaatatgc tgaccgataa actgcgcggt gaagcggaac tgaacaatag ccaacagtgg
    10741 cttgcattaa acattgaagc tggtttcccg gtgattgatg ccgccaacag cgggcagttt
    10801 atcccacagg cgaccaatct ccaggcgctg ggcggtatca gctttaagaa aggctgttat
    10861 accggacaag agatggtggc gcgagcaaaa ttccgtggtg ccaacaaacg cgcgctctgg
    10921 ttgctggcag gtagtgccag ccgactgccg gaagctggtg aagacttaga gctgaaaatg
    10981 ggcgagaact ggcgtcgtac cggtacggtg ctggctgcgg taaaactgga agatggtcag
    11041 gtcgtggtac aggtcgtcat gaataacgat atggaaccgg acagcatctt ccgcgtacgc
    11101 gacgatgcga atacactgca tatcgagccg ctgccgtatt cgctcgaaga gtaaatctct
    11161 gtatcgcatc aggcatttat cgcctgatgc gacgctgacg cgtcttatca tgtctgggat
    11221 ttgatgcctt tatcacaaca gggacgaggc ccgtagatcg gataaggcgt tcacgccgca
    11281 tccggcggtt gcgctccgat gcctgatgcg acgctgacgc gtcttatcag gcctacaaag
    11341 gcatacccat tacgcctgcc caatatacaa atagatcgcc agaaagtggc acacactacc
    11401 gccgagcacg aagccgtgcc agatggcatg gttgtatgga atgcgtttgc agacgtagaa
    11461 aatcaccccg agcgaataaa ccacgccgcc taccgccagt aaggtaacgc tgcccgccgc
    11521 gagcttaact gccatttcat aaattaccac cagcgacagc cagcccatcg ccagataagt
    11581 caccagagat aaaattttga atcggtgcgc gatggtcagt ttgaacagaa tccccagcaa
    11641 tgctaggctc cagataacaa tcatcaaccc gcgcgctaac ggagaatcca gccccaccag
    11701 caaaaacggc gtgtaggttc cggcaatcaa caggtaaata gcgcaatggt caaatttctt
    11761 cagccacatt tttgcccgtt gatgaggaat ggcgtgatag agcgtcgaag cgaggaacag
    11821 caggatcata ctgccgccat agaggctgta actggttatc gctgtggcgc tggcattgag
    11881 atccaccgcc tgaaccagta gcaacaccag cccaacgata ccaaacacca gcccaatgcc
    11941 gtgactgacg ctgttggcaa tttcctctgc cagcgaatat ccctgcttaa tgaggggctt
    12001 ctgaaccata acttactcca gagaaacgta cacgcacatg tatacctctc cagcgtaact
    12061 gagaatagtt ccagtgaaca cctgttagct aaaataaatt ttgatttgag actaatctcc
    12121 taaaaatcat gaaattaaat gcgaaatttc aactaacagg cgtcagttca atttaaagac
    12181 atttaaattc aatcatataa aattgtgtct gaccgggata gatgtcagca atgacctttt
    12241 tcagttcagg cagggtcata ttttcctgct ctgcatgttt ttccgtcagt gtatccagcg
    12301 ttacggttga ggttgcggtg acttcaatcg tgcaaaaata accgtcatct tcaaaacgtc
    12361 cgacacgaag cacatcaccc gttttgaagt gcgattcaga ctcgtcgcgg atggtgatgg
    12421 ttttacgccc agccagaatg tcatcctgga aacgttgaaa aaaagtgatg tcgtttggct
    12481 gcatggtact atttcctgta agaattgact gatctggagc ctatgatagt gaaaaaactc
    12541 accttaccga aagatttctt atggggtggc gccgttgccg ctcatcaggt cgaaggcggc
    12601 tggaacaaag gcggcaaagg accgagcatt tgtgacgttc tgaccggtgg cgcacacggc
    12661 gtgccgcgcg aaatcaccaa agaagtcttg ccaggaaaat actatccaaa ccatgaagcc
    12721 gttgattttt atggtcacta caaggaagac atcaagctat ttgccgaaat gggcttcaaa
    12781 tgttttcgga cttccatcgc ctggacgcgt atttttccaa aaggtgatga agctcagcca
    12841 aacgaagaag ggctgaagtt ttacgactct ctgttcgatg aactgctgaa atacaacatc
    12901 gaaccggtga taaccctctc ccactttgaa atgccgctgc atctggtgca gcaatacggt
    12961 agctggacca accgtaaatt cgttgatttc tttgtccgtt tcgcggaagt ggtattcgaa
    13021 cgctacaagc ataaagtcaa atactggatg accttcaacg aaataaacaa ccagcgtaac
    13081 tggcgtgcgc cgctgtttgg ttactgctgc tccggcgtgg tgtataccga gcatgacaac
    13141 ccggaagaga cgatgtacca ggtgctgcat caccagtttg tcgccagcgc cctggcagtg
    13201 aaagccgcgc gtcgcattaa cccggagatg aaagtcggct gtatgctggc gatggtgccg
    13261 ctctatccct actcatgtaa cccggacgat gtgatgttcg ctcaggagtc gatgcgcgaa
    13321 cgctacgtct ttaccgatgt gcagctatgc ggctattacc cgtcctatgt attgaacgag
    13381 tgggaacgtc gcggatttaa tatcaaaatg gaagacggcg atctcgatgt actgcgcgaa
    13441 ggcacctgcg attatcttgg tttcagctat tacatgacca acgcagtgaa ggccgaaggc
    13501 ggcaccggcg atgcgatctc tggttttgaa ggcagcgtac cgaacccgta tgttaaagcg
    13561 tctgactggg gctgcagatt gatccagtag gtctgcgcta tgccctttgc gaactgtatg
    13621 agcgttatca gaggccgctg tttattgtcg aaaacggttt tggcgcttac gacaaagtgg
    13681 aagaagatgg cagcatcaac gacgactacc gcattgacta cctgcgcgcc catattgaag
    13741 agatgaaaaa agcagtgact tacgatggtg tggatctgat gggctacaca ccgtggggct
    13801 gcatcgactg cgtgtcattc actaccgggc agtacagcaa acgctacggc tttatctatg
    13861 tgaataaaca tgacgacggt actggcgata tgtcgcgttc acgtaagaag agctttaact
    13921 ggtacaaaga ggtgattgcc agcaacggcg agaagcttta agtcgatgaa gtaccggatg
    13981 caatacttgt tgcatccggt cagacaactt ccctgtttta tttcccgccc gccaaatcga
    14041 taaaacttcc cgtgacgtaa gaggctttat cacttagtag ccagacaatg gcctgcgcga
    14101 cctcttctgc ctgtccacca cgctgcatgg ggatgttcga cttaacgcga tcgacgcgtc
    14161 caggctcgcc gccgctggcg tgcatttcgg tataaataaa ccctggccgc acgcagttaa
    14221 cgcggatccc ctgcgcggcg acttccagcg atcgtccggt ggttaacgta tcaatcgccc
    14281 ctttcgatgc cgcgtaatca acatattccc ctggcgaacc caaccgtgag gccaccgaag
    14341 agacattgac gatagcgccg ccactgccac catttttaag cgccatgcgt tttaccgcct
    14401 cgcggcagca gagaaaatat cccgtcacgt tggtggaaag tactcggttg attcgctctg
    14461 cggtaaggtt ttcaacggtg cactgggtaa acaagatccc ggcgttattg accagcgctg
    14521 ctagcggttc atcgtgctga tcgattgctg taaacatcgc aacgacctgg ttttcgtcgc
    14581 tgatatccgc ctggagcacg aatgctttgc caccggcttg cattattaag ttcatcactt
    14641 cctgcgccgc gtggaggttt tgctgataat taaccgccac cgtataccct tcttgcgcca
    14701 acagtaatgc agttgcccgc ccgatgccgc gactgccacc ggtcacaagt gctatagcca
    14761 tttgtttttc cgagaaaaat taattcgttg acgtatcttt gagattgcta acaaactggc
    14821 tcaatcttca atgttggcac gcttttcccc ctcgcccttt cagggagatg gtcggggtga
    14881 gggtaaatat tcgcgccagt gccggcctgt tcccctcacc ctaaccctct ccccaaaggg
    14941 gcgaggggac tgtctgagca cttttgtact ttgtcatctg actaaaaagg cgccgaagcg
    15001 cctttagaaa atactcgaat cagtgaatta ctggtattcg ctaatcggta cgcaggagca
    15061 gaacaggtta cggtcgccgt aaacatcatc cagacgtttc accgtcggcc agtatttgtc
    15121 tgccacgcct gccgggaata ccgcaacttc acggctgtac ggatgcgccc actcggcgac
    15181 cagttcgctc tgaatgtgcg gcgcgttcac cagcgggtta tcttccagcg gccagacacc
    15241 ggctttcacc tggtcaattt ctgcgcggat agccagcatc gcgtcgataa agcgatccag
    15301 ttccactttg ctttcagatt cagtcggttc aaccatcagc gtacccgcca ccgggaacga
    15361 catcgtcggc gcgtggaaac cgtagtcgat caggcgcttg gcaatatcca gctcgctgat
    15421 gccgatttct tctttcagcg ggcgaatatc gagaatacat tcgtgcgcca cgcgaccgtc
    15481 gcgaccggta tacagcaccg ggaaggcatc ctgcaggcgg ctggcaatat agttggcgtt
    15541 gaggattgcc acctggcttg cttttttcag cccttctgcg cccatcatgc ggatgtacat
    15601 ccagctgatt ggcaggatag aggcgctacc gaacggtgcc gcagaaactg cgccctgacg
    15661 ggttaacatg ccttcgattt gcaccacgct atgacccggt acaaacggtg ccagatgcgc
    15721 tttcacaccg atcggtccca tacccggacc accaccgccg tgcggaatgc agaaagtttt
    15781 atgcaggttg aggtgcgaaa cgtccgcgcc aataaagccc ggcgaggtga tgccaacctg
    15841 ggcgttcatg ttcgcgccat caaggtaaac ctgaccgccg aactgatgca cgacttcaca
    15901 cacttcacgg atcgtttctt catacacgcc gtgggtagaa ggataagtca ccatgataca
    15961 ggagaggtta tcgcccgcct gttccgcttt cgcgcgcaga tcagtcagat cgatgttgcc
    16021 gtttttatca cacgccacaa ccaccacctg cattcctgcc atatgtgcag aagcggggtt
    16081 agttccgtgc gcagaagccg ggatcaggca gatatcgcga tgcccttcgt tgcggctttc
    16141 atgataatga cgaatcgcca gcaggcccgc gtattcgccc tgtgcgccgg agttcggctg
    16201 catacaaacg gcgtcgtaac cggtcagttt caccagccag tcagccagct gcgcaatcat
    16261 ctgctgataa ccttcagcct gttccggcgg gcaaaacggg tgcagttcgg caaattccgt
    16321 ccaagtgatt gggatcatct cggcggcggc gttcagtttc atggtgcagg aacccagcgg
    16381 gatcatcgcc tgattcagcg ccagatcttt acgctccagc gagtgcatat agcgcatcat
    16441 ttcggtttcg ctgtgatagc gattaaacac cggatgggtg aggatttcgt cgtcgcgcag
    16501 catcgcaggc tggatagagc ggctgtcgtg agccacgtct ttgtccagcg tgtcgatctc
    16561 cagcccgtgg ttatcgccca gcagcacact gaaaagctgc attacgtttt cacgcgtggt
    16621 ggtttcatca agggtgatcc ccacggcgtt caggatatcg ctacgcaggt tgatttcagc
    16681 cgcttcggca cgcgtcagta cgcccgcttt gtcggctact tccacacaca aggtgtcgaa
    16741 atagtgcgca tggcgcagtt tcagaccttt ttgttgcagg cccgccgcca gaatatcggt
    16801 cagacggtga atgcggttag cgatacgttt caggccaacc gggccgtgat aaacggcata
    16861 caggctggcg atgtttgcca gcagtacctg ggaagtacaa atgttggagt tcgctttctc
    16921 acgacgaata tgttgctcgc gagtctgcat cgccatgcgc agcgcggtat tgccagctgc
    16981 atctttcgat acaccgataa tacggcccgg cattgagcgt ttgtattcat ctttcgccgc
    17041 aaagaatgcc gcgtgtgggc caccgtagcc catcggcacg ccgaagcgtt gcgccgaacc
    17101 aaaaacaata tccgcgccct gtttacccgg cgcagttaac agcaccagcg ccataatatc
    17161 ggcggcaacg ctgaccacaa ttttgcgtga tttcagttcg ctaataagcg cagtgtagtc
    17221 gtgaatttca ccggtagtgc ctacctgctg taacagcacg ccgaagacgt cctgatggtc
    17281 gagcactttt tgcgcgtcat cgacaatcac ttcaaaacca aaggtttcgg cacgagtacg
    17341 gaccacatcc agtgtttgcg gatgcacatt ggaagccacg aagaagcggt tggcattttt
    17401 cagtttgctg acgcgtttcg ccatcgccat tgcttcggcg gcagcggtgg cctcgtccag
    17461 aagagaagca gaggccatat ccagtccagt caaatccagc gttacctgct ggaagttgag
    17521 cagtgcttca aggcggccct gggagacttc aggttgatac ggagtgtacg cggtatacca
    17581 gcccggattt tccagcatgt tacgcaggat aaccggcggt agctgcacgg cggtgtaacc
    17641 catgccgatg taagacgtga agcgtttatt gcgactggca atagccttga gttctgccag
    17701 tgcggcgtat tcggtcgccg gtgcgccaac ctgcggtggt gtcgcaagct gaatatcttt
    17761 cggcacaatc tggccggtca gcgcgtttaa cgattgtgca ccaacggcat tcagcatttc
    17821 ttgctgttgc gcggcgtccg gtccgatatg gcgttcaata aaagcgccgc tgttttcaag
    17881 ctggcttaac gtctgtgtca tgagcgatgg ttcctgaaac gtgcagtgaa ttgtgaacct
    17941 ctctccttac gaagagagtg agggtgaggc ataaattttc ctcaccctga tcctctcccg
    18001 cagaagagga ataaagccgt tactcatctt ctaacaatgc ttcgtatgcg gtcgcatcca
    18061 gcagtgattc cagttcgctt tcatcgctag ctttgatttt aaagatccag ccgcctgcat
    18121 acggttcgct gttcaccagt tccggggaat cgctcagtgc gtcgtttacc gccacgattt
    18181 caccgcttac tggcgcataa atgtctgacg ccgcttttac cgattcggca accgcgcagt
    18241 catcgcccgc gctaaccgtt gcgcccactt ccggcaggtc aacaaacacc atatcgccta
    18301 acagctcctg agcatgttcg gtaataccaa cggtgtaagt gccgtcggct tctttacgca
    18361 gccattcgtg ttctttgctg tatttcagtt ctgctggtac gttgctcatc aatcaatctc
    18421 caaaaaagta aatcacgcga cggctttgcc gttacgcaca aaaacaggtt ttgtcacttt
    18481 aaccggcatt tcacggttgc gaatttgcac aatcgccgtt tcgccaatac cttccggcac
    18541 gcgcgccagc gcaatgctgt aacccagcgt cggggagaaa gtaccgctgg tgataatgcc
    18601 ttcatgctgg ttgccctgcg catcggtaaa gcgtaccggc agttcattac gcagcacgcc
    18661 tttttcggtc atcaccagac caaccagttt ttctgtacca tgctcacgct gcacttccag
    18721 ggcttcacga ccgataaagt cacgatctgt cggttcccag gcgatagtcc agcccatgtt
    18781 ggcggctaaa ggagaaatag tttcgtccat ctcctgacca taaagattca tgcccgcttc
    18841 cagacgcagc gtgtcacgcg cccccagacc gcagggctta acgcccgctt ccaccagcgc
    18901 acgccagaaa tcggccgctt tttcattggg cagcgcaatt tcatagcccg cttcaccggt
    18961 ataaccagtg gtggcaataa acagatcgcc cacctgcacg ccaaagaacg gtttcatccc
    19021 ttccaccgcc tgacgctgag catcattaaa cagtgtggca gcttttgcct gagcattcgg
    19081 cccttgcacg gcaatcatgg aaaggtcatc acgaacggta atttcgatgc cgaaaggttc
    19141 agcgtgttgg gtaatccagg agaggtcttt ttcgcgggtg gcggagttaa caatgaggcg
    19201 gaagaaatct tcagtaaagt agtagacgat gagatcatct atcacaccgc cagaggcatt
    19261 caacatcccc gagtaaaggg ctttgccgct tttggtgagc ttcgccacat cgttcgccag
    19321 cagataacgc agaaactccc gggtgcggct gccgcggaga tcgacgatgg tcatatgcga
    19381 cacatcaaac attccggcat cggtacgtac cgcatgatgt tcgtcgattt gcgaaccgta
    19441 atgcagcggc atcatccagc cgtggaaatc caccatgcga gcgccgcaaa gcgtgtgttg
    19501 ttcgtacaaa ggagtctgtt gtgccatctt gtcctcattg aataagcggg gctgacaact
    19561 ttttcatggt gaaattatca ccacaaaacc cagcatcgga gccactcccg gtccccaacg
    19621 caatcgttct cttttgcctg aacttaccac cgaaacagac tgttaaccat aaggtaaaat
    19681 tgatcatcac attagcttat ggttaaaaaa tgcaaaaatc gcgacagaat aaaaaaccaa
    19741 aaaatacacc aatttctata caaagatgat gtgatgagaa agtcaatttg aataagacaa
    19801 tattaagagc taaaaaaatg tcaaaaaaca ctaaatcaaa aaatcatggc attagaaaat
    19861 ataatgcgaa aacggaggtg aaattagttt atttcaaatg aggaaaatct cccggcgaaa
    19921 aaaccgggag atgaaagtgt gatgggtatc aaataaacaa cagaggagaa atttttaacg
    19981 cagccattca gacaaatcgt ttaatcccat tgcctggcgg ataagttgcg gcttaacgcc
    20041 aggaagcgtg tcggccagtt tcaaaccaat atcacgcagc agttttttcg ccggattagc
    20101 gccggaaaag agatcgcgaa aaccctgcat accagccagc atcagcgccg cgctgtgctt
    20161 gcggctacgt tcatagcggc gcaggtaaat gtactgcccg atgtctttac cctgacgatg
    20221 caaccgtttc agttcagcaa tcagctctgc ggcgtccata aatccgagat taacgccctg
    20281 ccccgccagt gggtgaatag tatgcgcggc gtcgcccacc agcgccagac ggtgtgcggc
    20341 aaactggcgc gcataacgcc ccgtcagcgg gaacacctga cgcgcgctct caaccttgca
    20401 taagccaagg cgattatcaa aagcgatatt taacgcgcga ttaaattcgt cttcacttgc
    20461 ctgctgcatc cgctgcgctt cctctggcga cagtgaccag acaatcgagc aaagatgcgg
    20521 atcgctaagc ggtaaaaagg ccagaatgcc ttcgccatgg aaaacctgcc gcgccaccgc
    20581 atcatgcggt tcttccgtgc gaatggtcgc taccagcgcg tgatgctgat aatcccagaa
    20641 agtcagcgga atatctgctt tgttgcgcaa ccaggaatta gcaccgtccg cgccaatcac
    20701 cagacgcgcc gttaacatgc tgccatcttt cagcgtcagg aaggtttcat tttctcccca
    20761 ggcgacctgc tgtaattctg tgggggccaa cagagtaata tctgacgact gctgcgcttt
    20821 gttccacagc gcgtagtgaa tcactgagtt ttcaacgatg tgcccaagat ggctatagcc
    20881 catgctttga tcgtcaaacg agatgtgacc aaaactgtct ttgtcccaca cttccatgcc
    20941 gtgatagcaa ctggccctac gagcgagaat gtcctgccag acgccaagac gggtgagtaa
    21001 tttttcgctg gcggcattga tagccgaaac gcgcagttgt ggtagtgcat ctaccgccag
    21061 aggttccggt acgcgctgct ccagtacggc aacgcgtaag ccgctcccct gtaagccgca
    21121 ggcaaccgcc agccccacca tgccgccgcc aacaatggct acatcaacac tttgcattgt
    21181 ttattcctta aaaccgcctt caacgcgcca cccaaccgag ggtgcgctgc gccagcacat
    21241 cgcgtgccgg ggtgaataat tccatcgtca tcagtccgat gttgcgcccg acaaccagcg
    21301 gtgcccaacg gttggcaaaa agatgtacca ggctgtccgt gacgccgatg gttgcttcgc
    21361 gatcgctctg tcgacgctgc tgatagcggc acaatatgcc gtaatcaccg atgtcttctc
    21421 cgcgctcatg cgcctgagtc agggtttccg caaggctcat gacatctcgc ataccgaggt
    21481 taaacccttg cccggcaatc gggtgcagag tttgcgccgc attgcccacc agcacagtgc
    21541 gatgggtaat cggtctggcg gcgcgggtta atgccagcgg ataagcactg cgtttaccgg
    21601 cgtgggtaat ttgccccagt cgccagccaa aggcagactg gagttcacgg caaaacttct
    21661 cgtcgctcca cgacagcacc tcttcgcgcc gttccagtgg atgacaccag accagcgaac
    21721 agcgtccgtc agacatcggc aacatcgcca gcgggccatg ttgcgtgaag cgttcaaaag
    21781 cgcggccttc gtgcgcaaca gaagtagcaa cgttggcaat cactgccagt tgttcgtaag
    21841 gctcctgctg ccaatcaacg ccgcacgcgg tggctaacgc tgaatgggtg ccatcagcgg
    21901 ctatcagcac gcggcccgtc agcatctcgc cgctctccag cgtcacttca acgtgactct
    21961 gagtacgggc aacgttagct acgcgatcgg ggcaatgcag cattacgcca ggtgcttttc
    22021 gcagcaatgc aaacagccgt tgcccaacat tatgcaattc gacaacctgc cccagcgccg
    22081 ccagttggta atcttctgcg gcgagggtga cgaatccagc atgaccacga tcgctgacat
    22141 gcacggtagt gatggcggtt gcgcaatcag ccagagattg ccagacgccg atgcgcgcca
    22201 gttgctgaca ggtacccgcc gccagcgcta tcgctcgtcc atcaaagccc ggatgagcat
    22261 gtgactctgg cgcagtcgct tcaatcaaat gtaccggcag cgccccgtga cttaaccggg
    22321 aaatagccag cgccagcgtc gcgcccgcca tgccgccacc gacgatgatt acgctcattg
    22381 ctttctcgca gcagccatca acgcttcgat ttcttccggc tttttcacca cgctggcggt
    22441 gaggttttcg ttgccggttt cggtaatcac aatgtcgtct tcaatacgaa tgccgatacc
    22501 gcgatattgt tctggcacat ccgcatccgg cgcgatatac agaccaggct ctacggtcag
    22561 taccatcccc ggttccagaa tgcgcgagcg atcctgacca taaacgccaa cgtcatggac
    22621 atccagtcct aaccagtggc taaggccatg cataaagaaa ggacgatggg cgttctgagc
    22681 gatcagttca tcaacattac ctttcaggat gccgagtttt accaggccgc taaccatgat
    22741 gcgcaccact tcaccagtga cttccagaat ggaagttccc ggacgataca ggcgcaggct
    22801 ggtttcgaga gactccagca caatgtcgta gatttcacgc tgggcctggg tgaatttgcc
    22861 gttgaccggg aaggtgcggg taatatcgcc cgcgtaacct ttgtattcac aacccgcgtc
    22921 aatcaacacc aggtcaccgt cgcgcagttc actctcgttt tcggtgtagt gcagaatgca
    22981 gccgttttca ccgctgccta caatggtgtt ataggaagga tagcgcgcac cgtggcggtt
    23041 aaattcgtgg tgaatttcgc cttccagatg gtactcgaac attcccggac ggcatttttc
    23101 catcgcccgt gtatgtgcca tggcggtgat ttctcccgcg cggcggagta cggcaatctc
    23161 ttctggcgat ttgaacaggc gcatttcatg aacaacagga cgccagtcga tcatcgttgc
    23221 cggtgcggtg agattttgcc gcgaaccttt acgcagtttt tccagcgcac tgttcacgat
    23281 ttcatcagca tatgcatatt cgccctgggc atggtaaacc acatccaggc cgttaagtag
    23341 ttgataaagt tgctgattga tttcgctgaa tgccagtgcg cggtcaacgc ccagtttctc
    23401 tggcgcggca tcctggccta agcgacggcc aaaccagatc tccgccgtca ggtcgcgaac
    23461 gcggttaaac agaacgctgt ggttatgagt gtcatcgctt ttaatcagca ccagcaccgc
    23521 ttccggttcg ttaaagccgg tgaagtacca gaagtcactg ttctgacgat aggggtattc
    23581 gctgtcggcg ctacgtgtta cttctggtgc agcaaaaatc agcgcggcgc tgccgggttg
    23641 catttgctcc accagggcct gacggcgacg ctgaaactct tgccgggata tctcactcat
    23701 aacactctcc ttacgttttt tgtttttagt gtagagtcgg tttttgtact tctggcgcgg
    23761 tcggttgcgg atgagtaaag gtgtcgtggc ataacagcgc ggcaacacga acgtattcga
    23821 tgatctcttc aagcgacatt tcaagctctt cctgatcttc gtcttcgtcg tagcccagct
    23881 gcgcgatgtt acgcagatcg tcgatggctt caccggtttc gccggtcact ttgtccagct
    23941 tcggttgcgt aacgccaaga ccaagcagga agtgattgac ccaacctgcc agtgcatcag
    24001 cccgatcgaa aacgctgaca tcatcgccat caggcagata aagctgaaaa aggaagccgt
    24061 catcctgcag ggcatcgctg gtggcagagt gcattttacg cagtgcctgt gccagctcat
    24121 gaccgaaagc catgccttcg ttcgtcaggt cgtgaagtag cggcagccat gagctgtcat
    24181 cgttaccgcc acatatcatc ccactgatta aaccatgcat ctcggcaggg gtcagacccg
    24241 tcccttgttg gttcagatac tggttcattt cgttgtaacc aggcatttcg ttctgtatag
    24301 acataagcat tcgtcgtcaa agggaggaat attcatgata tgctaccact ttgggccctg
    24361 gtggaccaga aaagggcttg tctcttctca tcagggtagc tatagcgtcg ccccttcgca
    24421 gaccatgggt ctaaagacga aggcagcgca gtcaatcagc aggaaggtgg catgtctgca
    24481 caacccgtcg atatccaaat ttttggccgt tcactgcgtg tgaactgccc gcctgaccaa
    24541 agggatgcgt tgaatcaggc agcggacgat ctgaaccaac ggttgcaaga tctgaaagaa
    24601 cgcactagag tcacaaatac tgaacagttg gtcttcattg ccgcattgaa tatcagctat
    24661 gagttagcgc aagaaaaagc aaagactcgt gactacgcgg caagtatgga acagcgtatt
    24721 cggatgctgc agcagaccat agaacaagcg ttacttgaac aaggtcgcat caccgaaaaa
    24781 actaaccaaa actttgaatg acacttttcg gtttactgtg gtagagtaac cgtgaagaca
    24841 aaatttctct gagatgttcg caagcgggcc agtcccctga gccgatattt cataccacaa
    24901 gaatgtggcg ctccgcggtt ggtgagcatg ctcggttcgt ccgagaagcc ttaaaactgc
    24961 gacgacacat tcaccttgaa ccaagggttc aagggttaca gcctgcggcg gcatctcgga
    25021 gattcccttc ttatctggca ccagccatga cgcaactacc agagctccca ctgacattat
    25081 cccgacaaga aatccgcaaa atgattcggc aacgtcgtcg tgcgttaacg ccggaacaac
    25141 agctggaaat gggtcaacaa gccgctaccc ggatgatgac ttatcccccg gtggtgatgg
    25201 cgcatacggt cgctgtattc ctctcttttg atggtgaact cgacacccag ccgctaatag
    25261 aacaactctg gcgcgccggt aagcgcgtat atcttccagt tttgcatccc tttagtgccg
    25321 gtaatttgct gttcctgaat taccatccgc aaagcgaact ggtgatgaac aggttgaaga
    25381 tccatgagcc aaaactggat gtgcgtgacg tgctacccct ttcccgatta gacgtgctga
    25441 tcacaccgct ggtcgccttt gatgagtacg gtcagcgcct gggaatgggc ggtggttttt
    25501 atgatcggac cttacaaaac tggcagcact ataaaacgca accggtgggt tatgcgcatg
    25561 attgtcagtt ggtggaaaaa ctccccgttg aagagtggga tatccctctt cctgcggtgg
    25621 ttacaccgtc gaaagtctgg gagtggtaag ggcgatacac ctgcatcgcc ctgattgaca
    25681 tcgttgattc tttaacctaa tttagtgagt aagggtaagg gaggattgct cctcccctgt
    25741 gactgttaat aagctgggaa acttatgagt aacagtacaa tcagtatgat ggcaagtcgc
    25801 atcataaccc ttctccttca agccctcgct tcggtgaggg ctttaccgtt acagccccat
    25861 gctgcccagc tatcgtaagc tctctttaaa taaccacaac gcattgatct acctttaaat
    25921 cttttcttcg agcgtgctcg aaaaacccca tgtaaaacag agataatttt tgggaaaaag
    25981 gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc ggttcagggc
    26041 gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt tagtgagtaa
    26101 gggtaaggga ggatgactcc tcccttgaga ctgactgtta ataagctccg aaacttatca
    26161 gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt taagccctcg
    26221 cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa atccccatta
    26281 aataaacaca acgcattgat ctgactttga tttattttct ggagcagact cgcaaagtag
    26341 aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt cagtgacctg
    26401 cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg gtacccggaa
    26461 tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca atatcaataa
    26521 ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg acgccctgct
    26581 cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg atgtgcatca
    26641 gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca gagagcgttg
    26701 agccattgtc agaatacttg atcaatttac ccgcaacttc caagccgata ttctcctgcg
    26761 cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat tcacacagtg
    26821 gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg attgccgccc
    26881 ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc acagtaccgc
    26941 gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc gcgcccatca
    27001 tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc atattcagca
    27061 ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt tcaatatcat
    27121 aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta ccaatatgac
    27181 cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc gccagtttgt
    27241 tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat agcagcagca
    27301 gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg ttaaataccg
    27361 ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata cagaaacagc
    27421 caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg gtacgggatc
    27481 gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca tccagcgcgc
    27541 ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc gccttttggt
    27601 gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc tttgccattt
    27661 acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc aacatatcaa
    27721 aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat atcacgcaaa
    27781 ggtaattctt ggatgaatat gctggtttag aggatttatt tagagcaatc gacaattgcc
    27841 tggtaaaagc gtgacacatg tcaccaaatt taatgaagag aattttttta acgggggagg
    27901 ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca atcagcgcaa
    27961 cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc gcatttatgg
    28021 cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg atcacgttgc
    28081 cattatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc accagctggc
    28141 gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat ttacccagaa
    28201 tatcaacctg cttggaggcg tcggcaatac agataaattt ttccgcaacc gaagcaatga
    28261 ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg ccgttgattt
    28321 catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca aaaacgtgaa
    28381 tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc ccttcaatct
    28441 ggcctttcat cgtgccgagc gcgtcaataa agtgtgcggc ggtggaacct gtacctacac
    28501 caacaatggt accgggctga acatactgaa gtgccgccca tcctactgct tttttcaatt
    28561 catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt atatacaaaa
    28621 aaagcgattc agaccccgtt ggcaagccgg gtggttaact catccataaa atatcgcgca
    28681 atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg tatgccgctc
    28741 cacggtgcga acagaggcaa taacgccacg ccattcaaca taaccagggc gcgaagtcgc
    28801 taagcgaaat gcaatagcgc aaatccctga tcttttaagc tgcgcgttta accattgata
    28861 caaatgttac ccgccgacgg cttcggtata tgcaacctga cacaaaattg tgtcatagtg
    28921 caggaaaaag catttaccag gagcagacaa cagcaatgaa acgcccggac tacagaacat
    28981 tacaggcact ggatgcggtg atacgtgaac gaggatttga gcgcgcggca caaaagctgt
    29041 gcattacaca atcagccgtc tcacagcgca ttaagcaact ggaaaatatg ttcgggcagc
    29101 cgctgttggt gcgtaccgta ccgccgcgcc cgacggaaca agggcaaaaa ctgctggcac
    29161 tgctgcgcca ggtggagttg ctggaagaag agtggctggg cgatgaacaa accggttcga
    29221 ctccgctgct gctttcactg gcggtcaacg ccgacagtct ggcgacgtgg ttgcttccgg
    29281 cactggctcc tgtgttggct gattcgccta tccgcctcaa cttgcaggta gaagatgaaa
    29341 cccgtactca ggaacgtctg cgccgcggcg aagtggtcgg cgcggtgagt attcaacatc
    29401 aggcgctgcc gagttgtctt gtcgataaac tcggtgcgct cgactatctg ttcgtcagct
    29461 caaaaccgtt tgccgaaaaa tattttccta acggcgtaac gcgttcggca ttattgaaag
    29521 cgccagtggt cgcgtttgac catcttgacg atatgcacca ggcctttttg cagcaaaact
    29581 tcgatcttcc tcctggcagc gtaccctgcc atatcgttaa ttcttcagaa gcgttcgtac
    29641 aacttgctcg ccagggcacc acctgctgta tgatcccgca cctgcaaatc gagaaagagc
    29701 tggccagcgg tgaactgatt gacttaacgc ctgggctatt tcaacgacgg atgctctact
    29761 ggcaccgctt tgctcctgaa agccgcatga tgcgtaaagt cactgatgcg ttactcgatt
    29821 atggtcacaa agtccttcgt caggattaat ccatcaaata atgcctgata gcacatatca
    29881 ggcgttgtcc tcacttcttt ttgtattcct tgaatcacat cacaaaatag acaa
//
LOCUS       DAJVQH010000038        28032 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJVQH010000038 DAJVQH010000000
VERSION     DAJVQH010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.038"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..1198
                     /gene="pdeC"
                     /locus_tag="OQM87_001772"
     CDS             <1..1198
                     /gene="pdeC"
                     /locus_tag="OQM87_001772"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCS1507935.1"
                     /translation="IYYYRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQW
                     GVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATIYSTKRPIAAIVATSY
                     QRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQP
                     IIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGA
                     YLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMT
                     PIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEH
                     IIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPAR
                     ELTRGQ"
     gene            complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="OQM87_001773"
     CDS             complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="OQM87_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCS1507936.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1610..2074
                     /gene="soxR"
                     /locus_tag="OQM87_001774"
     CDS             1610..2074
                     /gene="soxR"
                     /locus_tag="OQM87_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCS1507937.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2778..4127
                     /gene="ghxP"
                     /locus_tag="OQM87_001775"
                     /gene_synonym="yjcD"
     CDS             2778..4127
                     /gene="ghxP"
                     /locus_tag="OQM87_001775"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCS1507938.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4278..5927
                     /locus_tag="OQM87_001776"
     CDS             4278..5927
                     /locus_tag="OQM87_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCS1507939.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6081..7373)
                     /locus_tag="OQM87_001777"
     CDS             complement(6081..7373)
                     /locus_tag="OQM87_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1507940.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKENDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7551..9200)
                     /gene="actP"
                     /locus_tag="OQM87_001778"
     CDS             complement(7551..9200)
                     /gene="actP"
                     /locus_tag="OQM87_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCS1507941.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9197..9511)
                     /locus_tag="OQM87_001779"
     CDS             complement(9197..9511)
                     /locus_tag="OQM87_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCS1507942.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9711..11669)
                     /gene="acs"
                     /locus_tag="OQM87_001780"
     CDS             complement(9711..11669)
                     /gene="acs"
                     /locus_tag="OQM87_001780"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCS1507943.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12062..13498
                     /gene="nrfA"
                     /locus_tag="OQM87_001781"
     CDS             12062..13498
                     /gene="nrfA"
                     /locus_tag="OQM87_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCS1507944.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDKQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13543..14109
                     /gene="nrfB"
                     /locus_tag="OQM87_001782"
     CDS             13543..14109
                     /gene="nrfB"
                     /locus_tag="OQM87_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCS1507945.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14106..14777
                     /gene="nrfC"
                     /locus_tag="OQM87_001783"
     CDS             14106..14777
                     /gene="nrfC"
                     /locus_tag="OQM87_001783"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCS1507946.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14774..15730
                     /gene="nrfD"
                     /locus_tag="OQM87_001784"
     CDS             14774..15730
                     /gene="nrfD"
                     /locus_tag="OQM87_001784"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCS1507947.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            15750..17468
                     /locus_tag="OQM87_001785"
     CDS             15750..17468
                     /locus_tag="OQM87_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCS1507948.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSMLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17461..17844
                     /gene="nrfF"
                     /locus_tag="OQM87_001786"
     CDS             17461..17844
                     /gene="nrfF"
                     /locus_tag="OQM87_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCS1507949.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17841..18437
                     /gene="nrfG"
                     /locus_tag="OQM87_001787"
     CDS             17841..18437
                     /gene="nrfG"
                     /locus_tag="OQM87_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCS1507950.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18779..20092
                     /gene="gltP"
                     /locus_tag="OQM87_001788"
     CDS             18779..20092
                     /gene="gltP"
                     /locus_tag="OQM87_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCS1507951.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20170..20859)
                     /locus_tag="OQM87_001789"
     CDS             complement(20170..20859)
                     /locus_tag="OQM87_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1507952.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="OQM87_001790"
                     /pseudo
     CDS             complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="OQM87_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="OQM87_001791"
     CDS             complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="OQM87_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCS1507953.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="OQM87_001792"
                     /pseudo
     CDS             complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="OQM87_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26812..27750)
                     /locus_tag="OQM87_001793"
     CDS             complement(26812..27750)
                     /locus_tag="OQM87_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCS1507954.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKPEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADIALTKLHLEMTTVRAPFDGRIISLKTSVGQFASAMRPIFTLIDTRHWYVIA
                     NFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSI
                     NWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEPQ"
     gene            complement(27769..28023)
                     /locus_tag="OQM87_001794"
     CDS             complement(27769..28023)
                     /locus_tag="OQM87_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507955.1"
                     /translation="MAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWFFCAIASL
                     ILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         6375 a         7105 c         7560 g         6992 t
ORIGIN      
        1 tatctattat taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg
       61 gggaaattat gtggcggtta tcaaccctct cttctggagt gaagtgatgt ctgatgaccc
      121 gacattgcaa tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga
      181 ggcctcggca gcaacgtttt ctccgctgat tcatttgaag gatttaaccg tacaaagaaa
      241 tggctattta tatgcgacaa tttattcgac aaaacgccca attgcagcca ttgttgcgac
      301 ttcatatcaa cgtcttataa cccattttta taatcatctt atttttgcgt tgcccgccgg
      361 tattttgggg agtcttgttc tgctattact ctggctacgt attcgacaaa actatttatc
      421 tcccaaacgt aaattgcaac gcgccctcga aaaacatcaa ctttgtcttt attaccagcc
      481 aataatcgat atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg
      541 tgagcagggg caaataatga atccggcaga gtttattccg ctggcagaaa aggaggggat
      601 gatagaacag ataactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct
      661 ggcaacacat gccgatcgct atgtttctat taacctgtcg gcctccgatt ttcatacgtc
      721 acggttgata gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat
      781 taaatttgaa gtgactgagc atgcatttct tgatgtcgaa aaaatgacgc cgattattct
      841 ggctttccgc caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa
      901 cttgcataac cttaaatcat tgaatgtcga tattttgaaa atcgataaat cgtttgttga
      961 aacgctgacc acccacaaaa ccagtcattt gattgcggaa cacatcatcg agctggcgca
     1021 cagcctgggg ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct
     1081 gcgcaaacgc ggcgtgcgct attgccaggg atggtttttt gcgaaggcga tgccgccgca
     1141 ggtgtttatg caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa
     1201 ctacaggcgg tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga
     1261 gaaggtctgc tgcgagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt
     1321 ggtgcgcaac tcaacggcgg ccagtaacag gcggcgttgg cgaatgtaat cgccaagcgt
     1381 ctgatgcgtc accgtgcgga acatccgttg caagtaccac tttgaatagc ctgatttttt
     1441 tgcgacgaca tcaatgttaa gcggctggtc aatatgcacg tcaatccatg cgataagatc
     1501 ctgaataatt ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc
     1561 atctgttggg gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa
     1621 attaccccgc attaaagcgc tgctaacccc tggcgaagtg gcgaaacgca gcggtgtggc
     1681 ggtatcggcg ctgcatttct atgaaagcaa agggttgatt actagtatcc gtaacagcgg
     1741 caatcagcgg cgatataaac gtgatgtgtt gcgatatgtc gcaattatca aaatcgctca
     1801 gcgtattggc attccgctgg cgaccattgg tgaagcgttt ggcgtgttgc cagaagggca
     1861 tacgttaagt gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg
     1921 gcgcattcat accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg
     1981 cctttcgcgc agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac
     2041 cggcgcacgc ttgctggaag atgaacaaaa ctaaagcgcc acaaggacaa gctgactcat
     2101 cgttaactat gaggccgggg tgagggtaaa tatttgcact ggtgctgcct gttcccctca
     2161 ccctaaccct ctccccgaag gggcgagggg actgatcgag ctcttttgga attatccgca
     2221 aacacaaaac taaagcgcca caagggcgct ttagtttgtt ttccggtctt tgtctttctc
     2281 tctatcccgc tggaacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg
     2341 tggtggaggt tccggttggt gttgatgctt taattgtatg tcaccgacgt ttcttcgcca
     2401 gtgtaaaagt atacttttta gccgcaatat ttttgtcatc tcagacgatt ttttatcgca
     2461 atccagaacg gtatacggct cgataacgct gcaatcttgc acatcgacga taacgtttgc
     2521 gcatcaattg cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc
     2581 ggttgaaaaa gccgctatga tcgccggata atcgtttgct tttttaccac ccgttttgta
     2641 tgcgcggagc taaacgtttg cttttttgcg acgcaccaaa ttgccgcaaa cctggagcag
     2701 gaagataacg tttcgctggc aggggattgt ccgccacgca tcttgacgaa aattaaactc
     2761 tcaggggatg ttttcttatg tctacgccat cagcgcgtac cggcggttca ctcgacgcct
     2821 ggtttaaaat ttcacaacgt ggaagcactg tccgtcagga agtggttgcc gggttaacaa
     2881 cgtttctggc gatggtctac tcggtcatcg tcgttccagg tatgttgggt aaagcaggct
     2941 tcccgcctgc ggcagttttc gttgcaacct gtctggttgc cggactcggt tctatcgtga
     3001 tgggtctgtg ggctaatctg ccgttggcga ttggttgcgc catctccctg acggcgttta
     3061 ccgcattcag cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt
     3121 tcctgatggg ggtgctgttt acggtaattt ctgccacggg tatccgtagc tggattttgc
     3181 gcaacttgcc gcacggtgtg gcgcacggca cggggatcgg tatcggtctg ttcctgctgc
     3241 tcattgccgc taatggtgtc ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg
     3301 cgctgggtga tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg gcggtgatca
     3361 tcggcttgga aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa
     3421 ttgtcggttt gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat
     3481 tgagcgatga aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc
     3541 ctgtagtcct gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa
     3601 ctatccgcgc tgtcgccggc caggcgaacc tgctggataa agatgggcag atcatcgacg
     3661 gcgggaaagc actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgcag
     3721 ctccggcagc ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccgggt
     3781 tgacggctat caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctttctt
     3841 acctcgttcc ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga
     3901 gcaacgtggc gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg
     3961 cggtattcat cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc
     4021 tggtgattgg tcgtctggtt tccggtgaat ggcgcaagtt gaacatcggt acggtcgtta
     4081 tcgccgtggc gctggtgacc ttctatgcgg ggggctgggc tatctaatct ttcatcggat
     4141 tctgaaaacg ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt
     4201 tgcgatattc tgaaaaaatg agaattcagg cataacgtct ttctcagatc ataaaaaaca
     4261 tcgcaaacag ggaacgcatg gaaatcttct tcaccatact gataatgacc ctcgtggtct
     4321 cgctgtccgg ggtggtcact cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa
     4381 tcgccatcgg tgcgctactg gcgtggccga cgtttggttt gcatgtggag tttgatcctg
     4441 aactcttttt agtcttgttt atcccgccgt tgctgttcgc tgatggctgg aaaacgccga
     4501 cccgtgaatt tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg
     4561 tcaccgtggt cggcattggc ttccttattt actgggtggt gccgggcatt ccgctgatcc
     4621 ccgcctttgc gctggcggcg gtactttctc cgaccgatgc tgtggcgctc tccgggattg
     4681 ttggcgaagg gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga
     4741 atgacgcttc cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg gggacgatga
     4801 tcttcactgt cggcggtgcg acggtcgaat ttatgaaagt agccattggc ggtattcttg
     4861 ccggttttgt ggtgagctgg ctgtatggtc gctcgctgcg attcctcagc cgctggggcg
     4921 gtgatgaacc cgcgacgcag atcgttctgc tgttcttgct gccattcgct tcttatctga
     4981 ttgccgaaca tattggcgtt tcgggcatcc tcgctgcggt tgccgccggg atgaccatca
     5041 cccgctccgg tgtgatgcgc cgtgcgccac tggcaatgcg cctgcgcgca aacagcacct
     5101 gggcgatgct ggaatttgtc tttaacggca tggtgttcct gctgttaggt ctgcaactgc
     5161 cgggtattct ggagacgtcg ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga
     5221 tgctgtttac cgatattatt ctgatatatg cggcgctgat gctggtccgt ttcggctggc
     5281 tgtggacgat gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg gagtttggtt
     5341 cgtggaccac acgagaaatc ctgatcgcgt ctttcgctgg ggtgcgtggg gcgatcactc
     5401 tggccggtgt gctctcaatc ccgctgctct tgccggatgg taacgtcttc ccggcgcgct
     5461 atgagctggt gttcctggcg gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga
     5521 tgttgcctat tctgctacaa cacattgaag tcgcggacca ttcgcaacaa ttgaaagaag
     5581 aacgtattgc gcgagcggca acggcagaag tggcgattgt ggcgatccag aaaatggagg
     5641 agcgtctggc ggcggatacc gaagagaata tcgataacca gttgcttacg gaggtcagtt
     5701 ctcgcgtcat tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg
     5761 tgcaggaaga gaaccttgag cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg
     5821 aactgtacca cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa aaattactgc
     5881 acgatctcga tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg
     5941 taaccaggcc cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa caggttagct
     6001 cgggcgaaca ttcatgattc taaaacaaat aagttatctc tcttgtaaag tcaattcatt
     6061 ggctgttctt cgacttaacc tcaatctgcg tgttgcataa tatctgcagt aatcttcata
     6121 gcgactgcat taatattatc ctcagtttgt gacatgccag tatgagaggt acaatcttct
     6181 tcaaagaatt tctgtagtgt taccagggta tcactatcaa tggggatatt ttcctcccca
     6241 cggagaatgg aaatgcccat tccctggacg ctagcctcat cgaaattaac ccgatagaga
     6301 cgggtattaa acatacaggc ttttgttaaa atagaacagt tgaaatggca gttgcttaaa
     6361 ttggcattat taaaattaac attagccata ttagagccgg tacatataac catttcgagg
     6421 aatgtatttg tcagccacgt acaaaagagt ttgctatcaa ttaagtttat attctggagg
     6481 attgctttgt tcagattgac gcgagacaga tcagcatgtt caaagttaat taaatcgagt
     6541 ttagagaaac taaggtcggc tttaaataaa ttaacatatg gcgctattac tttataaaga
     6601 tttacctcag ccatgtacgc catataaaaa tttgaatagt caaggaaggt atttttaata
     6661 ttggctctat tcattgctgc attgtagaat tgaacatgca tagcggagca tgcatttaga
     6721 gttccgtcag ccatttttat ctgagcgaaa tttgagtcat cgagaataca attatcaaaa
     6781 agtatttttt ccattatggc gtaataaaaa ttcgttttat acatattaca gtcctggaaa
     6841 atagcgtttt gtagtctgca acctgcaaaa ttcgaattgg ataaatcttc atctttaatc
     6901 acgataccct tcaggctaag gtgtgaaaaa tcgtaaccac catttactat ctgtggaatt
     6961 tcgatagtat tattatggac ggcgacatta tttataaaat ataccacctt aatggataca
     7021 tcattttctt tactaaggta aactcttctc gtaattctat cgtcacattt attatttgca
     7081 tataacgcta tatctataaa atatttatgt attttcctgt ccgtttcttt cgtgattttt
     7141 cctgatggta aactatcata aattcttttc attttctgaa tcaagcctat cactttttca
     7201 tgatcatcta ttttgaaaag agagagggct ttattgtcaa ttaacgtgtt tttatgatgt
     7261 ttgctgtaat tatctgattt tgttttcttt gtatgtgatg aacttgtgat agagtggtta
     7321 tcgttaatct ggcaaagagg gaaaaacata ttatttaaac cattatagcg cataaaatat
     7381 ccttataatt aacaagaaag aaaaggcata ttctctgcat tatcattttc tacgactgtc
     7441 aaaaatcgct cattttttaa tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat
     7501 gttggagagg aagagtattc cccggtcagg cgaccgggga aggggagaga ttaatgtgcg
     7561 cggccttgct caacgccaaa gccggtctgg gagcggataa actgcgcgcg gaacagttca
     7621 cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat
     7681 gccacgctga tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgaccg
     7741 aggatctgta cccaaatagt cgggccgagg atcatcagca ccactgcggt aatcagcccc
     7801 agccagccgc ccagcatcgc gccacgggta gtcagtttcg accagtacat ggaaagcaga
     7861 atgatcggga agttacagct cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg
     7921 ttctgattct caaacagcac gccgaggata atcgcaatca cgccgaggat cagtacggtg
     7981 atttttgata cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac
     8041 aagtcatgcg aaaccgccga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata
     8101 gtggcgaaag caaccgctga aataaaaccg aggaataggt tgccgcccac tgcattcgcc
     8161 aggtgaacgg ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc
     8221 ggattcgcac caacaagcat gatcgcgccg aagccgataa taaaggtcag aatatagaag
     8281 tagcccataa atccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact
     8341 gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagcccc
     8401 agagagagcg cggagatcgg atctttcacc agtccgcccg gtttcatgat gtcgacacct
     8461 ttcgggtgta ccgccatcgc ttcgctgaac agattgttga agctaaagcc gacgtgtttc
     8521 atcaccataa aggccataaa gctggcaccg aacagcaaca gcacagcttt gataatttgt
     8581 acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc
     8641 agcaccaccg caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct
     8701 accatctgag cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata
     8761 cgaatcggcc cttgtttcag acgataagag gccacatcgg cagaggtgta gcgtcccagg
     8821 ttacgcagac gttcggtaat cagaaacaga atgattggcc agcccaccag gaagcccaac
     8881 gagtagatca gcccgtcata gccggaggta aacaccagcg cggagatccc caagaatgag
     8941 gcggcggaca tatagtcccc ggcaatcgcc agcccgttct ggaagccagt gatattgccg
     9001 cctgcggtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg
     9061 agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc
     9121 tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc
     9181 gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt
     9241 cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca
     9301 ctccaacacc aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca
     9361 gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa
     9421 tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt
     9481 cttctatccg ctgataaatc gtaccattca tcacagattc tccagaggta atgtagggat
     9541 tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg
     9601 taggccggat aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc
     9661 gtcttatcag gcctacaaac cgttaccgat tcgcatccgg caattgtggg ttacgatggc
     9721 atcgcgatag cctgcttctc ttcaagcagc ttctcgacca cgccaggatc ggcaagcgtc
     9781 gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat acggcgcata
     9841 attttgccgg agcgggtttt aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc
     9901 agcgggccaa tctctttacg cacccagttg cggacttctg cgtacagttc tggtgacggt
     9961 tcctccccgt gattaagcgt gacataggcg tagatcgcct gacctttaat attgtgcgga
    10021 atacctacta cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga ctctatctct
    10081 gccgtcccca gacggtgacc ggagacgttc agcacgtcgt ccacacgccc ggtgatccag
    10141 taatagccat cttcatcgcg acgcgcgccg tcgccgctga aatacatatt tttgaaggtg
    10201 gaaaaatagg tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc ctgaccaggc
    10261 caggagtcgg tgatcaccag gctaccttcg gtagcccctt ccagcgggtt accttcgtta
    10321 tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg
    10381 gtagcgccag gcagcggggt gatcatgaaa ccgccggttt cggtctgcca ccaggtatcg
    10441 accaccggac atttctcgtt gccgattttt ttccagtacc actcccacgc ttccgggtta
    10501 attggctcgc ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc ttcaatcgct
    10561 ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg cggtatagag aatattgacc
    10621 tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg tacgccttca
    10681 aacatcagcg tggtcgcgcc gcaggccagc gggccgtaca gcaaataact gtgtccggta
    10741 acccagccca catcggcggt gcaccagtag atatcacccg gatgataatc aaagacatat
    10801 ttaaaggtca gcgccgcgta caccagataa ccgccggtag tgtgcagtac gccttttggt
    10861 tttccggtag aaccggaggt atagagaata aacagcggat cttcggcgtt catctcttcc
    10921 gcctggtgct gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc
    10981 tgccagtcga tgtttccgcc agtacgcttc agtaccacca catgctctac gctggtgacg
    11041 ttcgggtttt ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg cccggcgcgc
    11101 acgccttcgt cggaagtgat caccagtcgt gagttggaat caataatgcg cccggcaacg
    11161 gctttcggcg agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg ggcgcaggcc
    11221 agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa tcgccaccac atcacctttt
    11281 ttaatgccca gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg cagctcttta
    11341 tagctgatat gtttgctctg gctggcgtcg tcgccttccc agatgatggc ggtgcgatcg
    11401 ccgttttctt gcagatggcg gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg
    11461 taccatttaa tggacacatt accgggggca aaggaggtat ttttcacctt ctggtacggt
    11521 ttgatccagt cgagaatttt tccctgttcg ccccagaagg tatcaggtac gttaatagat
    11581 tgttgataca tcgcctcgta ctgctgaggg tttatcaggc aacggtctgc gatgttggca
    11641 ggaatggtgt gtttgtgaat ttggctcatg cttttgttct ccttgtagga tgttaataat
    11701 atgtggcata agcgttaaat gtaggggtat tggcagtttt gtttagtatt tgggcgacag
    11761 atcacgcaaa agtagaatgg tgcaaataaa cggcagggta atttttgaag gtcaccaaca
    11821 aaagttgatt aattctttga ggaacatgca gttatgcatg ctgttgaaaa agaggaagat
    11881 actaactaac tctaaagtgg tattttacat gcacttacaa ttgattaaag acaacattca
    11941 cagtgtggtt atttgttaca cataggggtg agcaatgtca tgacagtgta ggtgcggtta
    12001 ctgtcgtgaa aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc
    12061 tatgacaagg ataaaaataa acgcacgccg tatcttcagc ttattgattc cttttttctt
    12121 tttcacttct gttcacgctg aacaaacggc agctcccgca aaacctgtaa ctgtggaagc
    12181 gaagaatgaa acctttgccc cgcagcatca cgatcaatat ctctcctgga aagccacctc
    12241 ggaacagtca gagcgtgttg acgccctgga ggaagatcca cggctggtga tcctgtgggc
    12301 ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga catgcctttg ctgtgaccga
    12361 tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat
    12421 ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag acggcgaaga
    12481 tggctacttc cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg
    12541 tgccgactgc cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct
    12601 ttcccgtccg tatgcggctc gcgcgatgga agccattggt aaaccttttg agaaagccgg
    12661 acgtttcgac aagcaatcga tggtttgcgg tcagtgccat gtggagtatt acttcgacgg
    12721 caaaaacaaa gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa atatggagca
    12781 gtattacgac aaaattgcct tctctgactg gactaactcc ctgtcgaaaa cgccaatgct
    12841 gaaagcgcag cacccggaat atgaaacctg gacagcgggc attcacggta aaaacaacgt
    12901 gacctgtatc gactgccata tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga
    12961 ccataaaatt ggtaatccgt ttgataactt cgcccagact tgtgcgaact gccataccca
    13021 ggataaagct gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta acgacctgaa
    13081 aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg cgctggatgc
    13141 aggcgcgacg gaagccgaaa tgaagccgat tcaggacgat atccgtcatg cccagtggcg
    13201 ttgggatctg gcgatcgctt cccacggtat tcatatgcac gcaccggaag aaggtctacg
    13261 gatgctcggt acggcgatgg ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct
    13321 ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat atctcgacca aagagaaagc
    13381 ccagcaggcc attggcctga acatggaaca aatcaaggcc gagaagcagg acttcatcaa
    13441 aacggtgatc ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa gccaataacc
    13501 ccgttccgcc tcgcaagggg cggaaaacac aatggagtga atatgagcgt attacgttcg
    13561 ttattaactg ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct
    13621 acccctgcgg tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg taacccggat
    13681 gccgcctgtc tggactgtca taaaccagat accgaaggta tgcatggcaa acatgcttcc
    13741 gttatcaacc cgaataacaa attgccggtc acctgcacca actgtcacgg ccagccatca
    13801 ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta acgagccgat gtacaaggtc
    13861 ggagagcaga acagcgtctg tatgtcctgt catctgccgg aacagttgca aaaagcgttc
    13921 tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca
    13981 cagcaagata cgatgcagac gttaagtgac aaaggacgga ttaagatttg cgtcgattgc
    14041 cacagcgatc agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt gcttaaggag
    14101 cagccatgac ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca
    14161 gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg cgttacggca
    14221 tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg tatggatgct tgtcgggaag
    14281 tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg
    14341 aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc gagcatgcgc
    14401 cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga tgccgctagc gggattgtcg
    14461 atgttaatcc tgatctctgc gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg
    14521 tgcgctttat ccatccggtc acgaagacgg cggacaaatg cgatttctgt cgtaagacca
    14581 atttgcaggc cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt
    14641 ttggcaatct ggacgatcct aacagtgaga tttcgcaact gctgcgccag aagcctactt
    14701 accgctacaa gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg
    14761 gggaggtgag ccaatgacgc agacttccgc atttcatttt gaatcgctgg tgtgggactg
    14821 gccgattgcc atctacctgt ttttgattgg tatttctgcc ggtctggtga cgctggccgt
    14881 gctgttacgt cgcttctacc cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac
    14941 gctgattgtc gggccgggcg cggtgatcct cggtctgttg atcctcgtct tccacctgac
    15001 aagaccgtgg accttctgga agctgatgtt ccactacagt tttacctcgg tgatgtcgat
    15061 gggggtgatg ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt
    15121 tgaatatgat ttgcttgccc tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa
    15181 ggttctgagc cttctgacgc ccgttcatcg cggactggaa acgctgatgc tggtgttggc
    15241 ggtgctgctg ggggcttata ccggctttct gctgtcggcg ctgaaatcgt atccgttcct
    15301 caataacccg agcctgccgg tgctgctcct cttctccggc atctcgtccg gtgcggcggt
    15361 ggcgctgatc gccatggcga tacgccaacg cagtaacccg cattccacgg aagcgcactt
    15421 tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt
    15481 tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg cattaggtgg
    15541 cggtttctgg acgtggtggt tctggcttgg tgtcgccggg ctggggctga ttgtgccaat
    15601 gttgctcaaa ccgtgggtca atcgcagttc cggcattcct gccgtgctgg cggcgtgtgg
    15661 ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg gacagttgac
    15721 ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt ccttcctgaa gtcggttttc
    15781 tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc gttgacggcc tttgcgggag
    15841 tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat cggcattctg gcgcagttcg
    15901 cgctcctgct gctcgccttt ggcgtactga cgtattgttt tctcatcagc gatttctcgg
    15961 tcatttatgt cgcccaacat agctacagca tgctgtcgtg ggaactcaaa ctggcggcgg
    16021 tgtggggcgg tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc gcctggagcg
    16081 cgctgtttgc ctggcattat cggcagcaaa gcgatccgct atttccgctg acgctagccg
    16141 ttttatctct catgctcgcc gcactgctac tgtttgtggt gctgtggtcc gatcccttcg
    16201 tgcggatatt tccaccagca atcgaaggcc gcgatctcaa tccgatgctg caacatcccg
    16261 gtcttatctt tcatccaccg ctgctttacc ttggctatgg cggtttgatg gtagcggcga
    16321 gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct
    16381 ggcgctgggc gttacctggc tggagtgcat taacggcggg gatcatcctc ggttcctggt
    16441 gggcctactg cgaactcggc tggggcggct ggtggttctg ggatccggtg gaaaacgcct
    16501 ctttattacc ctggctttct gccactgcgc tgctgcacag tttgtccctg acacgccagc
    16561 gggggatttt ccgccactgg tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc
    16621 tgggcacctt aattgtccgt tctggcattc tggtttcggt tcatgcgttc gcactggata
    16681 acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat tagccttgcg tctttggctc
    16741 tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg
    16801 aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg
    16861 gaacgcttta tccgatgatt tacggcctgc tgggctgggg acgcctctcc gttggcgcgc
    16921 cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg attgtgctgg
    16981 cgacgtttgt ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc
    17041 tgttatttgc cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc agcctgaatt
    17101 tacagccggg tcagcaggtg acgctggcag gatacacctt ccgttttgag cgactcgatc
    17161 tgcaagccag aggcaattac accagcgaaa aagcgatagt ggcgctgttt gaccatcagc
    17221 aacgcattgg tgaattaacg ccggagcggc gtttttatga agcacgccgt cagcaaatga
    17281 tggaaccgtc aattcgctgg aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa
    17341 ccagagcgga tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg
    17401 ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg
    17461 atgaataaag ggcttctcac gctactgctg ttatttacct gttttgcgcg cgctcaggtt
    17521 gtagacacct ggcaattcgc caatccgcaa caacagcaac aggcgttaaa tattgccagc
    17581 cagttacgtt gtccgcagtg ccagaatcaa aacttactcg aatccaatgc gccggtggcg
    17641 gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc
    17701 attggctgga tgaccgaacg ctacggagat tttgttcgct ataacccacc gttaacgggt
    17761 cagacgctgg tgttgtgggc gctgccagtg gtgttgttat tgctgatggc actgatcctc
    17821 tggcgagtga gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac
    17881 tcacgatatt gatggtattc ctctgtgtcg gcagttatct gttaagccca aaatggcagg
    17941 cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa
    18001 ccccggaagc gcagcttcag gcattgcagg ataaaatccg cgctaatcca caaaacagcg
    18061 aacaatgggc gttactgggc gagtactatc tgtggcaaaa cgattacagc aattcgctgc
    18121 tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc tgaactgtat gcggcgctgg
    18181 cgacggtgct ttattaccag gccagccagc atatgaccgc ccagactcgc gcaatgatcg
    18241 acaaagccct cgcgctggac tctaacgaaa tcaccgctct gatgctgctg gcttccgatg
    18301 cgtttatgca ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg atggatctca
    18361 actcaccccg gatcaaccgg acacagctgg tcgagtcgat taatatggca aaactgttgc
    18421 agcggagatc ggattaatca tctgttgatg aagattatgt cattgagctg cataaaaaat
    18481 aatcgaatga acatatgcca aaaataatca ccaatcagta ttattgcaga ttaaacaaat
    18541 aaaaatcttt ccataacaaa tggttattca ttaatcctgc cataaactcg cgttattatg
    18601 cattaatgca gcgaaaagct ctgttgttaa agggttgcgc aacataccgc gcaaatgata
    18661 ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg ccctggcagt
    18721 atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc attgaggaag ttattctgat
    18781 gaaaaatata aaattcagcc tggcctggca gattctgttt gctatggtgc tgggcattct
    18841 cctgggaagc tacctgcact accatagcga cagccgcgac tggctggtcg tcaatttgct
    18901 ctctccggcg ggtgatatct tcatccatct gatcaaaatg attgttgtgc cgattgtgat
    18961 ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg
    19021 cgcgaaaacc attatttact tcgaggtgat caccaccgtc gccatcattt tggggatcac
    19081 tctggcgaac gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga
    19141 tatctcgaaa tatcagagca ctacggaagc ggtacaaagc agttcccacg gcattatggg
    19201 cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct
    19261 gccgatcatc tttttctcgg tgctgtttgg tctggggctt tcttccctgc ccgcgacgca
    19321 tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa accatgttta aagtgactca
    19381 catggtgatg cgttatgcac cggtgggtgt gtttgcgctg attgcggtga cggtggctaa
    19441 ctttggtttc tcgtctctgt ggccactggc gaaactggtg ctgctggtgc atttcgccat
    19501 tctgttcttc gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg
    19561 gagcctgatt cgtattctga aagatgagct gattctggcg tactccactg ccagctctga
    19621 aagcgtactg ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac
    19681 cagcttcgtg gtgccgaccg gttactcttt taaccttgat ggttcgacgc tgtatcaaag
    19741 tattgccgct attttcatcg ctcagctgta tggcattgac ctgtccatct ggcaggaaat
    19801 cattctggtg ctgacgctga tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc
    19861 gtttgtggtg ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt
    19921 tattgctggt gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa
    19981 tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct
    20041 ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat aattgaagat
    20101 tgccggggat atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc
    20161 aggcctacct taaccgttgg ttaatttttc aaactcttca cacccggtat caaacccttc
    20221 catacagctc aggttcaacc agtgcagcgc cttttgtttg ttcttctcga taaaaccttc
    20281 ctcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc gggaaattgc
    20341 agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt
    20401 ggcgtaaatc aaaccaagca gcatttgggc atcgacggcg gagtcattct ccagatcttc
    20461 cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt
    20521 taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc
    20581 tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt tgatctgcgc
    20641 caacagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc
    20701 tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgttggtc
    20761 cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg cgtgggcaaa
    20821 gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag
    20881 ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta
    20941 gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc
    21001 gtcaatcagg tactgctcgg cggcgcgctg atcggagatc ggctcgacgc gcacggcgca
    21061 gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca
    21121 ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac
    21181 ctgcgcacgg gtgataattt tgcctttacg cgagtgcact caaaccaatg cctcatcttc
    21241 aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag
    21301 cgccgccagt gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg
    21361 caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc
    21421 gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga
    21481 agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc
    21541 cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat
    21601 ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt cactgatgat
    21661 ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc
    21721 acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac
    21781 atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc
    21841 ttcaaaggct ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat
    21901 aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata
    21961 gccggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc
    22021 cggatctttc acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc
    22081 accctgcacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt
    22141 accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg
    22201 ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc
    22261 ctgacgaatc tcactggcgc tgacaccggt gatatcttca accgactccg gcgtgtagcc
    22321 ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc
    22381 gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc
    22441 gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca
    22501 gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca cgattgggtg
    22561 ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat
    22621 agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg
    22681 tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg
    22741 cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc
    22801 cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc
    22861 tcaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg
    22921 cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct tcagacacag
    22981 ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac
    23041 gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat
    23101 cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt
    23161 tatgccacat tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc
    23221 tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc
    23281 atcacgcggc agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc
    23341 agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat cagtaatgac
    23401 atctcttcgg caagtactgg caaccgggct tcagtggcct gtaagcggct ggttaagccg
    23461 cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc
    23521 tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc
    23581 accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg
    23641 ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg
    23701 aagttgaact ggcgactggt ttttttgaat aaggtatcta ggtggatggc atcaaggccg
    23761 aaaaacgctt tgatatcaaa gctcggatag aacaacgccc gcgcggaatc cacctgatct
    23821 aatgacgcct gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca
    23881 taagagagtg tcgccggaat gccggtctgg actcgtggta atgccacagg tttgatctcc
    23941 ggcatatcgc tggctcccgc gccaatcaat gcgcgcagag attctctcgt ttcggtgatt
    24001 tgccctttga cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg
    24061 aaaggcactt gtgcttccag accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa
    24121 tcaatcacat cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg
    24181 taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc
    24241 gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca
    24301 agatccagtc cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccatcccc
    24361 agtgcgggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac acgttggcgg
    24421 ttaagcatcc ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa atcggcctgc
    24481 gattgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc
    24541 cgttggatca gggcatccaa ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc
    24601 cagccggagc tggcaagatg aatatcatcg gccagtttga tttgttccgg tttgagctgt
    24661 tgatgaggcg cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg
    24721 agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg
    24781 cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct
    24841 ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag gcgggtgtct
    24901 cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca gcgtatttct
    24961 ccagggcgtc ggcaaactga cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg
    25021 ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga ataaccgttt
    25081 gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc
    25141 gttggcacat ctcttcacag gcattaaacg ccgcatgcag accgatacga atttgcagat
    25201 aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta cttaacatac
    25261 ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc actttcaggc cagacaaagg
    25321 tgtaaatcat cgcggacacc acggtaccaa tgatgatacc cagggcgcga tcgcgaattt
    25381 ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca
    25441 ccatctgtgt gccgatataa gaagagcgtt cagagctggt ggcaatctat gcgcccaaca
    25501 ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag ggcatgacca
    25561 gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca
    25621 tcttctggta cgacgaaccg acatttggat tagcgacgat cacgcaggtc agcatacagg
    25681 tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta gcagatcaaa caggcgagca
    25741 atgtttttac cgcgtagcgt atatagtctg gattggtaaa agcatcggcg accattgatg
    25801 gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg
    25861 tctggcagat attctccagg ttacattccc gtgccgccac cgcttcactt tcactgatcc
    25921 gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagcttg ttgatttctg
    25981 aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag gtgggatcgt
    26041 agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc caccatgcgc
    26101 tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt tgcagcgcca
    26161 gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa
    26221 tggcatcatc aagcagatca ttaagcgctt gatgcatttg cgtaatggca cgattgggaa
    26281 accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca acgatacacc
    26341 acagcgttaa gcgcacgact acttccggat agtcgagcat ggcggggaag gtttgcccgt
    26401 aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa
    26461 acatgcagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg
    26521 accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc acaaacaaga
    26581 tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc actgccagcg
    26641 ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc aggcagccta
    26701 cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa
    26761 agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc attattgcgg
    26821 ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag gtttatcgac
    26881 catgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac gagacacttt
    26941 tggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc caatcgaatc
    27001 cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc
    27061 tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc agtgacgagt
    27121 gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg aggttttgag
    27181 ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc atttccagat gtagtttggt
    27241 cagggcaata tccgcttctg cggcgcgctg cgccgttctt gcacggtcaa cgtcttctgc
    27301 tgagacaaaa ccctctttca gtaatggctc ggtgcggcgt aatgtatctg tggcctgttt
    27361 cgcggcggca cgggcttttt ctaccgtggc attaaccgag tcggcaccaa actgttgggc
    27421 gtcaacgcta cgctgggtga gcataatttg cttatccagc gccgcgaggg aggcttcggg
    27481 tttcgccagg ttggcttcgt acgggcgcgg gtcgatgcgg aacagcaaat cgccctgttt
    27541 caccgcctgg ttgtcggtga ccgccagttc tacaatgcga ccgctgactt ccggcaccac
    27601 atcaatggta tctgctgacg cgtaagcgtc attagttgat ggcgcactgt ctacgcgcca
    27661 gataacgaaa acaagggcaa ccagcgccaa cgcaaccact aacagagcag ggaatttact
    27721 gcgaggtgct tttttcggcg tactttccat gagcgcgtct caaatagatt agaaaaatgc
    27781 cagccagaac agcatggcgt agagagcaaa aagggcggta taaataattc cgacaaatgc
    27841 cagattgata tttgcccgct gaataactcg cctcgtgatg agcgtcaaaa taaggctggc
    27901 aatggcgcag aaaaaccagc tgggataata agcgccgatc accgggattg ccggagataa
    27961 tgagcatccc gaaaccatga agacgggaat tatccaggct gtttttttga ggtgtatcgc
    28021 catgcgagag ag
//
LOCUS       DAJVQH010000039        28027 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJVQH010000039 DAJVQH010000000
VERSION     DAJVQH010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.039"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(100..390)
                     /gene="ubiK"
                     /locus_tag="OQM87_001795"
     CDS             complement(100..390)
                     /gene="ubiK"
                     /locus_tag="OQM87_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCS1507956.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
     regulatory      512..660
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            764..1417
                     /gene="ribB"
                     /locus_tag="OQM87_001796"
     CDS             764..1417
                     /gene="ribB"
                     /locus_tag="OQM87_001796"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCS1507957.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            1678..1848
                     /locus_tag="OQM87_001797"
     CDS             1678..1848
                     /locus_tag="OQM87_001797"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507958.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(1906..2679)
                     /gene="zupT"
                     /locus_tag="OQM87_001798"
     CDS             complement(1906..2679)
                     /gene="zupT"
                     /locus_tag="OQM87_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCS1507959.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            2822..3610
                     /gene="ygiD"
                     /locus_tag="OQM87_001799"
     CDS             2822..3610
                     /gene="ygiD"
                     /locus_tag="OQM87_001799"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCS1507960.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(3648..4808)
                     /locus_tag="OQM87_001800"
     CDS             complement(3648..4808)
                     /locus_tag="OQM87_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1507961.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(4814..5485)
                     /locus_tag="OQM87_001801"
     CDS             complement(4814..5485)
                     /locus_tag="OQM87_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCS1507962.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(5633..7114)
                     /gene="tolC"
                     /locus_tag="OQM87_001802"
     CDS             complement(5633..7114)
                     /gene="tolC"
                     /locus_tag="OQM87_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCS1507963.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQACLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7319..7948
                     /gene="nudF"
                     /locus_tag="OQM87_001803"
     CDS             7319..7948
                     /gene="nudF"
                     /locus_tag="OQM87_001803"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCS1507964.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7949..8371
                     /locus_tag="OQM87_001804"
                     /pseudo
     CDS             7949..8371
                     /locus_tag="OQM87_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
     gene            8396..9223
                     /gene="cpdA"
                     /locus_tag="OQM87_001805"
     CDS             8396..9223
                     /gene="cpdA"
                     /locus_tag="OQM87_001805"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCS1507965.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9223..9804
                     /gene="yqiA"
                     /locus_tag="OQM87_001806"
     CDS             9223..9804
                     /gene="yqiA"
                     /locus_tag="OQM87_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCS1507966.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            9833..11725
                     /gene="parE"
                     /locus_tag="OQM87_001807"
     CDS             9833..11725
                     /gene="parE"
                     /locus_tag="OQM87_001807"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCS1507967.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11773..12087)
                     /locus_tag="OQM87_001808"
     CDS             complement(11773..12087)
                     /locus_tag="OQM87_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS1507968.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12118..12699)
                     /gene="mdaB"
                     /locus_tag="OQM87_001809"
     CDS             complement(12118..12699)
                     /gene="mdaB"
                     /locus_tag="OQM87_001809"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCS1507969.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            13018..13350
                     /locus_tag="OQM87_001810"
     CDS             13018..13350
                     /locus_tag="OQM87_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCS1507970.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            complement(13396..14745)
                     /gene="qseC"
                     /locus_tag="OQM87_001811"
     CDS             complement(13396..14745)
                     /gene="qseC"
                     /locus_tag="OQM87_001811"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCS1507971.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14742..15401)
                     /gene="qseB"
                     /locus_tag="OQM87_001812"
     CDS             complement(14742..15401)
                     /gene="qseB"
                     /locus_tag="OQM87_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCS1507972.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            15553..15945
                     /gene="ygiW"
                     /locus_tag="OQM87_001813"
     CDS             15553..15945
                     /gene="ygiW"
                     /locus_tag="OQM87_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCS1507973.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            15998..16480
                     /locus_tag="OQM87_001814"
     CDS             15998..16480
                     /locus_tag="OQM87_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1507974.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            17026..19284
                     /gene="parC"
                     /locus_tag="OQM87_001815"
     CDS             17026..19284
                     /gene="parC"
                     /locus_tag="OQM87_001815"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCS1507975.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            19518..20255
                     /gene="plsC"
                     /locus_tag="OQM87_001816"
                     /gene_synonym="parF"
     CDS             19518..20255
                     /gene="plsC"
                     /locus_tag="OQM87_001816"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCS1507976.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            20330..21742
                     /gene="ftsP"
                     /locus_tag="OQM87_001817"
     CDS             20330..21742
                     /gene="ftsP"
                     /locus_tag="OQM87_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCS1507977.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21853..24070
                     /locus_tag="OQM87_001818"
                     /pseudo
     CDS             21853..24070
                     /locus_tag="OQM87_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
     gene            complement(24113..24370)
                     /gene="yqhH"
                     /locus_tag="OQM87_001819"
     CDS             complement(24113..24370)
                     /gene="yqhH"
                     /locus_tag="OQM87_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCS1507978.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24421..25347)
                     /locus_tag="OQM87_001820"
     CDS             complement(24421..25347)
                     /locus_tag="OQM87_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCS1507979.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNNNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            complement(25547..26374)
                     /gene="dkgA"
                     /locus_tag="OQM87_001821"
     CDS             complement(25547..26374)
                     /gene="dkgA"
                     /locus_tag="OQM87_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCS1507980.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKCPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            complement(26479..27642)
                     /gene="yqhD"
                     /locus_tag="OQM87_001822"
     CDS             complement(26479..27642)
                     /gene="yqhD"
                     /locus_tag="OQM87_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCS1507981.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKAAR"
     gene            27836..>28027
                     /locus_tag="OQM87_001823"
     CDS             27836..>28027
                     /locus_tag="OQM87_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1507982.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYIN"
BASE COUNT         6567 a         6946 c         7442 g         7072 t
ORIGIN      
        1 ttgtgatgat actctgaatg ggattaagcg ataatgtaat agaaaacact gtaaattaga
       61 ggattaattc ataatttaca gtgcattaaa gacaaatgtt tacagcgttg gggggagagt
      121 ctctggatct ggctgctttt tgatctcagt actgcgattt tccagttcgc tgattctttg
      181 ttcaagcagc gccagtttct cacgagtccg taacaggact tgcgtttgga cgtcgaactc
      241 ttcacggctt acaaggtcga ggcgcgtcag ctgagcttgt agggtttggc ggattttttt
      301 ctccacatct tccccgaact ccctgattcc tttaggcatt gattcgtgaa cctggcgagc
      361 gatttgctca atttttttcg ggtcaatcat tgtagtttcc ctgtactgat aggtgttgag
      421 taccattgta gtgcgataag ggtaagtcat aaaccagaat tatgtgaagc tatgcgttgc
      481 tgccgctaat cattagagtt atagtgaatc cgcttattct cagggcgggg cgaaattccc
      541 caccggcggt aaatcaactc agttgaaagc ccgcgagcgc tttgggtgcg aactcaaagg
      601 acagcagatc cggtgtaatt ccggggccga cggttagagt ccggatggga gagagtaacg
      661 attctgtcgg gcatggaccc gctcacgtta ttttggctat atgccgccac tcctaagact
      721 gccctgattc tggtaaccat aattttagtg aggttttttt accatgaatc agacgctact
      781 ttcctctttt ggtacgcctt tcgaacgtgt tgaaaatgca ctggctgcgc tgcgtgaagg
      841 acgcggtgta atggtgcttg atgatgaaga ccgtgaaaac gaaggcgata tgatcttccc
      901 ggcagaaacc atgaccgttg agcagatggc gctgaccatt cgccacggta gcggtattgt
      961 ttgcctgtgc atcactgaag atcgccgtaa acaacttgat ctgccaatga tggtagaaaa
     1021 taacaccagc gcctatggca ccggttttac cgtgaccatt gaagcggctg aaggtgtgac
     1081 taccggtgtt tctgccgctg atcgtattac gaccgttcgc gcagcgattg ccgatggcgc
     1141 aaaaccttct gatctgaatc gcccgggcca cgttttccca cttcgcgcgc aggcaggtgg
     1201 tgtactgacg cgtggcggtc atactgaagc gactattgat ttgatgacgc tggcaggctt
     1261 taaaccggct ggtgtgctgt gtgagctgac taatgacgat ggcacgatgg cgcgtgcacc
     1321 agagtgcatt gagtttgcca ataaacacaa tatggcgctc gtgactattg aagacctggt
     1381 ggcataccgt caggcacatg agcgtaaagc cagctgaaaa ccgctgctta attactgcct
     1441 taatcaagaa accgaagttg tagctggctt cggtttttat tttgccctgc tatgcaagat
     1501 gttaattccg cttctctttc tatgagaaaa tttcattaat atcaggcatt ctttttcatt
     1561 atgatcaaaa tcatataatt gttccgttat tatgcgacca tatgaatata tccgaaaaag
     1621 gatatgtgaa atttatgcgc taaatgttta gtattgttaa atattaaggg taaaaggatg
     1681 tttatcgcct ggtactggat tgtattgatt gctctggttg tggtgggtta tttcctgcat
     1741 ttgaaacgtt attgtcgggc gtttcgccag gacagagacg cactgcttga agcgcggaac
     1801 aaatacttaa acagtacgag agaagagaca gccgaaaagg tagaataagg ctttcgtgaa
     1861 gctggcagaa gttgtccatg tgctggacaa cttctgatgt tgtttttaac caattcccgc
     1921 cgtttgtagc agcactaaac tgaatcccat cactgacatt ccacatagta cgccatagct
     1981 ggggttatta ttagggtcaa tctctttggc gagcggcatt aattcatcaa ccgagagcgc
     2041 caccataatt cctgcaaccg ccgccatgat tgccgccatg accaccgggg aaatcatgct
     2101 accgaggatt aaccacgcca gcacaccacc aagaatttct gctagtccag aaatccccgc
     2161 ccacagaatc gcggtgcgtt tagaccccgt tgccgcataa accgggcctg ccactgccag
     2221 accttcaggg atattgtgca acgcgacggc cagtgcgatg ccaaatccca gctccaggtt
     2281 gctgctcgcc gtgacaaagg tggcaatccc ttccgggaag ttatgcagac tgatgccgag
     2341 agtgagcaga atggctgtgc gcttgatcga ttttggcaac agctgcaccg atttttgcat
     2401 taaatcctgc ggatgcgcat gcggcaacat gcggtccagg ccaaagtagc caagcagacc
     2461 gaagataaac attccataac ccaacaccgg cgacattcct tcagctgcta gtgcggcagg
     2521 aagcatttcc attaatgaga tgagcaacat gatccccgcc gcaaaaccta gcgaaaacgc
     2581 cagtaagcgg ttcgagggtt tttgtccgag aacgccgaga aacgcgccaa taaacgtggc
     2641 tgcccccgcc agtatggtca gaatgagagg tactgacatc gacaactcct tataaatctt
     2701 caccttcagg cagatcgtca aaataactga tgatcatcat cattgttatc cgggttctta
     2761 ctccgccgaa caagcgtatt gtgaggatat caaaatgaca cctttagtta aggatatcat
     2821 catgtcttca acacgtatgc cagcattgtt tttaggtcac ggtagtccga tgaacgtgct
     2881 ggaagataat ttgtataccc gcagctggca gaagttgggg atgacattgc cacgcccgca
     2941 agcgattgtg gtggtttcgg ctcactggtt tacccgtgga acaggagtga ccgcgatgga
     3001 gacgccgccc acgattcatg actttggtgg cttcccgcag gcgctgtacg atacgcatta
     3061 tcctgctccg ggttcgcctg cgctggcaca gcgtctggtt gagctgttag cgccgatccc
     3121 ggtgacgctg gataaagaag cctggggctt tgaccacggc tcgtggggcg tgctgattaa
     3181 gatgtatcct gacgccgata tccctatggt gcagttgagt atcgacagta gcaaacctgc
     3241 cgcctggcat ttcgaaatgg ggcgcaaact cgcggcgttg cgtgatgaag gaataatgtt
     3301 ggtcgccagc ggtaacgtgg tgcataacct gcgcacagtg aagtggcacg gtgatagttc
     3361 accgtatccg tgggcgacgt cgtttaatga gtatgtgaaa gcgaatctga cgtggcaagg
     3421 gccggtagag cagcatcctc tggtgaatta tctcgaccat gaaggtggcg cgttatcgaa
     3481 cccaacgcca gagcactatc tgccgttgtt gtatgtgtta ggtgtgtggg atgggcagga
     3541 gccaattacc attccggtcg atggtataga aatgggcagt ttaagtatgc tgtcggtgca
     3601 gataggctaa caattaccgg atggcgagtg ccatccggta tcgtggctta ttcaacaaaa
     3661 atatgtggat aaaaccgaga catatcctgg gtgatcaacg cacggtcttc acgaatgcca
     3721 attccggcag gttgatcgtt caccagccag ctaccaatca gcatatagct gtcgccgaat
     3781 ttcggtaacg ggtggaattg ctgaacaatc atcccttctt cgccatacgg accttccgct
     3841 gcttcaatgg ttttgccgtt ctcaatgatc gacacgtttg cgccttcacg ggagaagatc
     3901 ggtttaacca catatttttc catttgcgga tgatcatctt ccgcaaaata agcgggcagc
     3961 aggttcgggt gattcgggaa catctcccac agtagcggta gaagtgcctt gttggagata
     4021 atactcttcc acgccggttc cagccagcgt acgccagcat cttccagctt ggtggagaac
     4081 atttcgcgca gcataaattc ccacggataa agcttgaaca ggttagaaat cacctgatcc
     4141 tgtaaatccg tgaactggcc tttttcgcct aaacctatct cgtcgatgta gaggaactcg
     4201 gtggcgattt cagcttcggt tgcgcagtcc tgcaaatact gaatggttcc gcgatcttcc
     4261 accgtgtcgc gacagcaggt gagatgcagc aactggaagc cacactgttc acgcagctca
     4321 acgaagcgat cgatcagttt ttcttgcaga ctgttaaact ggtcgctgcc ctccggcaag
     4381 ttaccggcgt taagctgatc ttccagccag atccactgaa agaacgccgc ctcgtatagt
     4441 gacgttggcg tatcggcgtt attttccaga agtttaggtt cgccagtgcc atcccacgcc
     4501 agatcaagac gcgaataaag cgatggctgg tgagtcagcc atgattgacg cacaaaactc
     4561 caggtgtgtt ttggtatgcg gaatttggtc atcagctcat cgctggcgat cactttttcc
     4621 accactttca ggcacatctg gtgcagttcg gcggtgactt cttccagctt ttcaacctgg
     4681 gcgagggtca acttgtagta agcatcttca caccagtacg gctcgccgta catggtgtga
     4741 aaattgaaac cgtattcgtg ggctttctca cgccagtccg ggcgctcggt aatactgact
     4801 ctttccatcg gtatcagcca cccattgaac gagaagaggt gccggttgcg ctacgctgca
     4861 tagtgctttg tttggcaaca gattcaccaa aaccgccacg ggtaacggta gtggtggtcg
     4921 ccggttttgg tgccattgcc gtcttcggta cggtcatggt gcggcctggc tgggctgcgc
     4981 catagttttt acccgtcgcg tcggtatatt taccgtaagc cggactggcc gggtttttcg
     5041 aggagaacag cggctgctgt gcaaatcccg cgccgccgcc catcagacgc cccatcatgt
     5101 aaccggccat cagcggcatc cagaaactcc cgctggattg ctgagcctgc gcctggtttt
     5161 ctggtgccat gccagcctgg gctggtgctt gctgacactg accttcacca aattcagcaa
     5221 cacagtcttc acgggtggcg tatttcggcg cagtacgttc ggcttctttc agcgcattgt
     5281 tgtacgcggt ggtacattcg gcgcttttgc ctgggtttgc agctgaacag tcgtcagcat
     5341 tttgatagag agacactgtt tcatcactct tttcacagcc agccagcata aaaacagtgg
     5401 caaccgcgag agcgactggt gtcagatggc gtgcgctcca gtttttgcgg aacgatgcgt
     5461 ggcgtatgga ttttgtccgt ttcatttttg tcttccggga ccagtggtaa atacccatca
     5521 gaatagagga tggctggtcg aaattgaagc gagaaggggg aagaatgcgg cagataaccc
     5581 gtatctttac gttgccttac gttcagacgg ggccgaagcc ccgtcgtcgt catcagttac
     5641 ggaaagggtt atgaccgtta ctggtggtag tgcgtgcgga tgtttgctga acgacgggtg
     5701 ccgggctatc aggcgcataa ccatcagcaa tagcattctg ttccggcgtt tgcggggcaa
     5761 cgttttccgg attagtggaa accggtttgc tcagcgcatt gttcagtgcc agcaaatcct
     5821 gctcgttcaa cgtacccagg gctgacttaa tattcagctg attaatcagg tagttataac
     5881 gcgcattcgc cagctcttgc ttggcgttgt acagcgtggt ggtcgcatcc aacacatcaa
     5941 caatggtacg cgtaccgacc gagtagcccg cttccatcgc gtctaatgag ctttgcgcgg
     6001 aaactacggc ttgtttgtag gcgttaatac tactgataga agcattaatg ttgttgaagg
     6061 aggaacgtac ggtttgcacg acgctacgat gtgcgctttc cagttgctca ctggcaccaa
     6121 caaagttgta ctgtgcctgt ttcacctgcg agttaaccat tccgccctga taaatcggca
     6181 gcgagaagct caggccaact ttgttctggc ccatattgct atcgtcatac tgggtaccag
     6241 cggcaccacg ggttttcgaa ccgctataag aggtgtcaga aatcccggta gaagccgtta
     6301 aatccagagt cggtaagtga ccatcctgcg cctggcgaat ttgctcgcgc gccaggtcct
     6361 ggctcaagca tgcctgtaac agcgacaggt tgcgtttttc ggcttctttc agcagcgcgt
     6421 taaccggctg tggtttgtcg gttttaaagt tttcgacatt cagcgccgcc agttccggat
     6481 agtagttacc ggtgatctgg cgcagctgct ctaccgcgtt atcaaggtta ttacgtgcgg
     6541 tcacttcgtt cgccagcacg gtatcgtact gcgcgcgggc gttctgcacg tcggtgatcg
     6601 ctaccaggcc cacgttaaaa cgttgggtgg tttgatctaa ttgacggtag atcgcttctt
     6661 tttgtgcctg tgtataggaa agaacgtcaa tagcattcaa cacgttgaaa taagcggtcg
     6721 cggtgttgag gatcaaggtt tgctgatcgg tctgatatgt gacgtcctga atccctgctg
     6781 ctttttcctg cagcgttaac gcacgccatt tcgacatatc aaaaatggat tgagttaact
     6841 gcagggacgc actggtcgcg ttcgagttga tgccgttcgc gtcgcggtag ccgttgctat
     6901 aggtgtaatc tgcacctaaa cctagctgtg gcagtaatgg actgcgcgct tcattaattt
     6961 tttcaaaggc agcatcacga tcggcggcag acttacgcaa ttccgggtta ctaaggcgtg
     7021 cttgctgata aacttgcatc aggttctcgg cctggctcaa cgaactgaac ccagaaaggc
     7081 tcaggccgat aagaatgggg agcaatttct tcatttgcat tccttgtggt gaagcagtat
     7141 ttagcgcgat caaactgtaa aattattgcg gattctagca gaagccgcta ccgcaaaagg
     7201 ttggcgttac gtgccatagg gcgttaatgt gcctcaattt aacatatgac tgctcaaacg
     7261 gcagtcaaac gtcgctgaaa ttcacattta attcactatt agtgccagga cattaacaat
     7321 gcttaagcca gacaacctgc ccgttacatt tggcaaaaac gatgtagaaa ttattgcacg
     7381 agaaacactt tatcgcggct ttttttcatt agatctttat agatttcgtc atcgtctatt
     7441 caacgggcaa atgagtcatg aggtacggcg ggaaattttt gagcgcggtc acgccgcagt
     7501 cttgctaccc tttgacccag tgcgtgatga agttgtgctg attgagcaga ttcggattgc
     7561 cgcgtacgac accagcgaaa ccccctggct actggagatg gttgccggga tgattgaaga
     7621 gggtgaaagt gtggaagatg ttgcccgtcg cgaagcgatt gaagaggcgg gactgatagt
     7681 caaacggacc aaaccggtgt taagtttcct ggcaagcccg gggggcacca gtgagcgttc
     7741 gtcaattatg gtgggcgaag tggacgccac gaccgcaagc ggtattcatg gtctggctga
     7801 tgaaaacgaa gatattcgcg ttcatgtggt aagccgggaa caggcatacc agtgggtaga
     7861 agaggggaaa atcgacaacg cagcgtcggt catcgctttg caatggctgc agctgcatca
     7921 tcaagcgtta aaaaatgagt gggcataaat gaagcgttac acacctgact ttcctgaaat
     7981 gatgcgcctg tgcgagatga acttttcata attgcgccgt ttgttaccgc gcaattacgc
     8041 acccggcgaa actgtaagct atcaggtggc aaacgcacaa tatcggctga cgattgtgga
     8101 atcgacccga tacactaccc tggtgacaat agaacagact gcgcccgcga tcagttactg
     8161 gagccttccg tcaatgacgg tgcgtctgta tcatgacgcg atggtggctg aagtgtgttc
     8221 aagccagcag atttttcgct tcaaagcgcg gtatgattat cctaataaaa agttgcatca
     8281 acgcgacgaa aagcatcaaa ttaaccagtt tttagcggac tggttgcgat actgtttagc
     8341 acatggagcg atggcgattc cggtttacta gcgtcgtgaa acctaaggac accatttgga
     8401 aagcctgtta acccttcctc tggctggtga ggccagagga aggattttac aaattaccga
     8461 tactcacctg tttgcacaaa agcacgaagc cctgttaggg gtaaacacct gggaaagcta
     8521 ccaggcggtg ctggaggcga ttcgtccaca ccagcacgaa ttcgacctga ttgtcgcgac
     8581 aggtgattta gcgcaggatc aatcctctgc ggcctatcag catttcgctg aaggcatcgc
     8641 aagttttcgt gcgccctgcg tctggctgcc gggcaaccac gatttccagc ccgcgatgta
     8701 cagcgcgtta caggatgcgg gtatctcccc ggcgaagcgg gtgtttattg gtgagcaatg
     8761 gcaaatcctg ttgctggata gccaggtgtt tggcgtgccg cacggtgagc tgagcgagtt
     8821 tcagcttgag tggctggaac gtaaactggc cgatgcgcca gaacgccata cgttgctgct
     8881 gctgcatcat catccgctac ctgcgggttg tagttggctc gatcaacaca gtctgcgtaa
     8941 cgcgggcgaa ctggataccg tgctggcgaa gtttccgcac gtcaaatact tgctgtgcgg
     9001 tcatattcat caggagctgg atctcgactg gaacggtcgc cgcctgctgg caacgccgtc
     9061 gacctgtgtg cagtttaagc cgcactgttc caactttacg ctggatacta tcgcgcccgg
     9121 ctggcgtact ctcgagttac atgctgatgg cacgctgacc accgaggtgc atcgcctagc
     9181 ggacacacgt ttccaacctg ataccgcttc agaaggctac tgatgtctac gcttctttat
     9241 ttacacggtt tcaacagctc gccgcgctct gcaaaagcga gcttgttaaa aaactggctg
     9301 gcggaacatc accctgacgt tgagatgatc attccgcagt tgccgccgta tccttccgac
     9361 gcggcagaac tgctggaatc cattgtcctg gaacatggcg gcgattcgct gggtattgtc
     9421 ggttcgtcac tggggggata ttacgccacc tggttgtcgc aatgttttat gctgcccgca
     9481 gtggtggtaa acccggcggt acgcccgttt gaattgctga cggactatct cggtcagaac
     9541 gagaacccct acaccgggca gcaatatgtg ctagagtcac gccatattta cgatctcaaa
     9601 gtcatgcaga ttgacccgct ggaagcgccg gatttgatct ggctgctgca acagacggga
     9661 gatgaagtgc tggattaccg ccaggcggtg gcgtactacg cttcctgccg ccagactgtc
     9721 atagaaggcg gcaaccacgc attcacgggc ttcgaagatt atttcaaccc gatcgtcgat
     9781 tttcttggtc tgcaccatct ctgacgatca actcgaattg ctaacttaaa ccatgacgca
     9841 aacttataac gctgatgcca ttgaggtact caccgggctt gagccggttc gccgccgtcc
     9901 ggggatgtat accgatacca ctcgccctaa ccatttgggg caagaagtta ttgataacag
     9961 tgtcgatgaa gcactggcgg gtcacgcaaa acgcgtggat gtaatcttac atgctgacca
    10021 gtcgttagaa gtgattgacg atgggcgcgg gatgccggtg gatattcacc cggaagaagg
    10081 agtaccggcg gttgaactga ttctttgccg tctgcatgca ggcggtaaat tctctaacaa
    10141 aaattaccag ttctctggcg gcctgcatgg cgtggggatt tcggtagtta acgccttgtc
    10201 gaagcgcgta gaagttaacg tgcgccgcga tggtcaggtt tataacatcg cctttgaaaa
    10261 tggcgaaaag gtgcaggatt tacaggttgt cggcacttgc ggtaaacgca ataccggtac
    10321 cagcgtgcac ttctggccgg atgaaacctt ctttgacagc ccgcgttttt ctgtttcacg
    10381 cctgacgcat gtgctgaagg ccaaagcagt actgtgcccc ggcgttgaga ttacctttaa
    10441 agatgagatc aacaacaccg aacagcgctg gtgctatcag gacggtctga atgattacct
    10501 ggcggaagcg gtaaacggtt taccgacgct gccagaaaaa ccgtttatcg gtaatttcgc
    10561 tggcgatact gaagctgtgg actgggcgct actgtggctg ccggaaggcg gtgaactgct
    10621 gaccgaaagc tacgtcaacc ttatcccaac gatgcagggc ggtacccatg ttaatggtct
    10681 gcgtcagggc ctgttggacg cgatgcgtga gttctgtgaa taccgcaaca ttctgccgcg
    10741 cggcgtgaag ctgtcggcgg aagatatctg ggatcgctgc gcctatgttc tgtcggttaa
    10801 aatgcaggat ccgcagtttg ccgggcagac caaagagcgt ctctcttcgc gtcagtgtgc
    10861 ggcattcgtt tcgggcgtgg tgaaagatgc cttcatcctg tggctgaacc agaacgttca
    10921 ggcggcggag ttgctggcag agatggcgat ttccagcgcc cagcgtcgta tgcgtgcggc
    10981 taaaaaagtg gtgcgtaaaa agctgaccag cggcccggcg ctgcctggca aactggctga
    11041 ttgtaccgcg caggacctta accgtaccga actgttcctc gtggaaggtg actccgcagg
    11101 cggatctgcc aagcaggcgc gcgatcgtga atatcaggcg atcatgccgc tgaaaggtaa
    11161 gatccttaac acctgggaag tttcttccga cgaagtgctg gcctcgcagg aagtgcatga
    11221 tatttcggta gcgatcggta tcgatcctga cagcgacgat ctgagccagc ttcgttatgg
    11281 caaaatctgt atcctcgcgg atgcggactc tgatggtctg cacattgcca cgctgctctg
    11341 cgctttgttc gtaaaacact tccgcgcgtt ggtgaaacac ggtcacgttt acgtcgcact
    11401 gccaccgctc taccgtattg atctcgggaa agaggtttat tacgcgctga cggaagaaga
    11461 gaaagagggc gtacttgagc aattaaaacg caagaaaggc aagccgaacg tccagcgttt
    11521 taaaggtctg ggggaaatga acccgatgca attgcgtgaa accacgcttg atccgaacac
    11581 tcgccgtctg gtgcagttga ctatcgatga tgaagacgat cagcgtactg acgcgatgat
    11641 ggatatgctg ctggcgaaga aacgctcgga agatcgccgc aactggttgc aagagaaagg
    11701 cgacatggcg gagattgagg tttaaaggaa agaacattgc ccggtcagga tggccgggca
    11761 aacaaggcag gattaaatcc ctggctgcag aatacggata ttcatctcca gcacgtcgcc
    11821 ttttacggct ttgctgtacg ccttcatgtg cggggtttgc agatgcgctt caaggtgcgc
    11881 gatgctttcc cactgctcaa tcatcacgat agaatccggt gccatagact ggaaactcac
    11941 gccagcagcg caatccacca ttggcgcata gccgtggcaa ccttcttctt tcagtacggt
    12001 tggaacgatt ttagcaaact gatccaatac cgcctgacgg tgatgttgac caggacgagt
    12061 acggatttct gcgattacgg taagcatggt taactccttc taaagcctga gctctagtta
    12121 accaaaaatt tccacaagat gcttgcgata ttcttcagta tagcggggaa catcaggcat
    12181 ttttatcacg tcattagcga taaatgtcgg cagcggttcc ataccgagga attggtttgc
    12241 tttatggaac ggcagataca caccgtcaac gccaacgccg tggaagaact gatctttttc
    12301 ggtgaaggct tccattggtg cgttccaggt tagagaaagc atatattttt tgccctgtac
    12361 caggccgccg gaaccgtatt ttttcgacgg atctttgcgg gtacgaccat cgctggcata
    12421 cagagtcccg tgaccttcgg tgaatacatc atcaatatat tttttcactg tccacggcgc
    12481 gcccatccac cagcctggca tctgccagat caccacatca gcccagagaa agttttgtac
    12541 ttccgctttg acattgtagt cgctgtcggc gcgaacgatg cggacatcat gcccgaggtc
    12601 gcgcagtgtg ccatccgcga cttcggtcag ggtgtcgttc agttgaccat tggagtgggc
    12661 gaattttttc gcgccgttga taatcaggat gttgctcatt ttttaacctc aaggtgagac
    12721 gtttgtcgac tatagtacgt cacagagcgg tacggtgaaa ttagcaaaat gtgcaaagtc
    12781 ttttgcgaat ttagcaaaag tctgatctag ctctgactca ccgatgcagg ataaatattt
    12841 tctgaaaatg acaatactga tagccattcc tcataattct tactcgtacc ggaataattt
    12901 acacgtttgg ttcggttttt ttggggattg aaaatatggc tttgaataag taaaagatga
    12961 aaatatcaac gtgtcatgtt gataattata aatggatttt tatgagcgta ctgatatatg
    13021 ttaaagcaga aaattaaaac aatatttgaa gcattgctat atataatgct tacttattgg
    13081 ttaatagatt ctttttttgc tttcaataag tatgactgga tgcttgagtc gggaggcaat
    13141 atttgcagta taccttccgt ttcaggcgaa gatcgtatat tgcaagcaat gattgcagca
    13201 ttttttcttc ttacgccact tattattctt atcttaagaa aactctttat gcgggaaatg
    13261 ttcgagtttt ggctatatgt attctcgctc gttatttgtc tggtttgtgg ttggtggctt
    13321 ttttggggac ggtttatatt ttgctattag taaaatctat gagagtcgtt ttaacggctc
    13381 tcatagacag agaagttacc agcttacctt cgcctcaaat ccaccttgtt ccgcattccc
    13441 aaactcaaca ttcatgtcat gcagcttggc gattcgctgg acaatcgata gcccaagtcc
    13501 actgccggta gcggtttgtc cgggtgggcg atagaagcgt tcgccaattc gcgccagtgc
    13561 ctctggtgtc acaccggggc cgttatccct cacgatgaaa ttatcagcat tcagcgtgac
    13621 gtctatcacg ctgccctgtg gactgtagcg cacggcgtta tccagcaaat ttcgcaccaa
    13681 caaacttagc aatagcggtt gtccggtgcg tttgatgccg tgggcattga gtgtcagtcg
    13741 cacgtcaatt ttcgcctgct gcgccgtgtg gtaaatatcc atcaccgacg attgtaggag
    13801 atcttcaagc gggatctccg cgacgtcctg aaggttatcc agtgagtcca gccgcgatag
    13861 cgtgagcagt tgatcaacca gacgagtagc gcgatcgatc ccggaatgta attggagcag
    13921 tgcttttttc cgcgcctgcg gatcatcgtc agagagctgc gcaacttcgg tttgcacttt
    13981 cagcgctgtt aacgggctac gtagttcgtg agctgcatcg gaggtaaagc gtcgttctcg
    14041 aaccatcatc gcatgtgtgc gggcgaacag ttgatttagc gactcaacca gtggccgcac
    14101 ttcgctgggt acgccagtcg cgtttagtgg tttttccgag tcagggtcac gcatacgtag
    14161 tgccagcgcc agtttgttca gcggcgcgag ttcacgaccc agtagtacca tcatgatgat
    14221 taacataatc ggcagtgcga ccagccacgg gatcaattgc ccggcaacaa tcgccagcgc
    14281 catgtcttca cggtattccc attcctggcc aacaacgatg cgatatttgc catcaggtga
    14341 ggtcatccag acaaaacgcc aaggatcgtc ttcaccgacc agttgcccgt cagcaaaacc
    14401 ttcccgttga tagctatagg gaatatcttc tccgttatcg ccatcattaa ggaccattct
    14461 gccgtcgtgg gtaaagatgg caaaggtcag cgcatcgtca tcaacatgac cgtgttttaa
    14521 tttatttggc gtctgtgcca tgcgatccgc cgcgttgatt tcgttgagat cgagcgtact
    14581 taaccgtttg gcaaacagca tcagttgggt gtcgaacaat tcatcgacgt tatccgttgt
    14641 ttgtttccag gcgacaaagc tggaaagcag ccaggtcacc gaggccagaa ttaaaaagat
    14701 tagcgtcagc ctgacgcgca gactaagacg ttgggtaaat ttcatttctc acctaatgtg
    14761 tagccaatac catgcacggt acgaataaaa tcactaccga gtttgcgtcg cagatgatgc
    14821 acatgcactt caacggcatt actggtgacc tcttcgtccc aggtatacag tttctcttca
    14881 atcagtttgc gcggcagtac ccgaccagcg ttacgcatca gtaattccag cagggcaaat
    14941 tcttttggtt tcagtgttaa gggttcgcca gccagcgtgg cgatacgttt gccggggtcg
    15001 agcatgacgt taccgtggcg cagctcgttg ctggcctggc cgttggttcg gcgcatcaga
    15061 gcctccagcc tggcggcgac ttctatcaac gcaaaaggtt tacacagata atcgtcagct
    15121 cccagacgca gcccttctac acgttccgct aacgcatcac gcgcggtcag gttcagtacc
    15181 ggctcacgct gacctttctc tcgccattcg cgcaaaatat cgcgaccatc catgcctggt
    15241 aaggttaaat ccaggatcac cgcatcataa ggtgcgctat aaagcgcctc ttttccctga
    15301 cgaccttgtg taaaccagtc gacactaaaa cccattttac taaggccagt tttgatgccg
    15361 tcaccaatca gcatgtcatc ttctatcagt aaaattcgca ttttttcatc cctgcgataa
    15421 ccgtatttgc tgccagtaaa ccgtgttgta acgcgctctg tacagcatta aattttattt
    15481 ttttccctta agaagttgtt aaggactatc ttgttaaatg ctcggaaaca gacattaaag
    15541 ggagtaataa acatgaaaaa attcgcagca gtaatcgcag taatggccct gtgcagtgca
    15601 ccggtgatgg cagcagagca gggcggtttt tctggcccat cggcaacgca aagtcaggcc
    15661 ggaggatttc aggggccgaa cggcagcgta acgactgtag aaagcgtaaa atccctgcgt
    15721 gacgacacct gggtaaccct gcgcggcaat atcgttgaac gcatctctga cgatctctac
    15781 gtgtttaaag atgccagcgg tactatcaat gttgatatcg atcacaaacg ctggaacggt
    15841 gtgacggtga cgccgaaaga tacggttgag attcagggtg aagtcgataa agactggaat
    15901 tctgttgaaa ttgacgtcaa acagatccgc aaagtaaatc cgtaattgtt accgctcccg
    15961 ggacgcgttc ccgggaataa tttcgcaggg aggcaaaatg acaaacctga cactggatgt
    16021 aaacattatc gatttcccat caatccctgt ggcgatgttg ccgcaccgct gtagccctga
    16081 gttgctcaac tacagtgtgg cgaaatttat catgtggcgt aaagagacgg ggctttctcc
    16141 tgttaaccaa agccagactt ttggcgtcgc ctgggacgac cctgccacca ccgcaccgga
    16201 agcgtttcgc tttgatatct gcggcagcgt tagcgaaccg attcccgata atcgttatgg
    16261 tgtgagcaat ggtgaactta ccggtggacg ttatgccgtg gcccgccacg ttggcgagct
    16321 ggacgatatt tcacacacga tatggggcat tattcgccac tggctgcctg caagcggcga
    16381 gaaaatgcgt aaagcaccga ttctgtttca ctacaccaat cttgccgaag gggtgacaga
    16441 gcagcgactg gaaacggatg tttatgtgcc gttggcgtga tggggaatct ttaccggagt
    16501 gtgagccaaa tcactcgcga taagtttgag cgaacaatgt cacaggcgta ttgttgttgg
    16561 gcttataatt tctccggtaa ggaataccat gtatacgcga aatttattat ccggtgcgcc
    16621 gtaagccgtc tcgggggtat ccatgagcaa gccgcactta tggcaggagg cgcggggcac
    16681 tggtggagac acagctaaac tgggctgaac cgtatactta cagtcgacta ctttgatgct
    16741 ttaggcttcc ctggactcgc ataacctcaa cttctcgaat cgcccggagc ggacccccat
    16801 gccgagtggt gtggcatggg cgcaaccttg tgctgccctc tatgccgatc aagtctacag
    16861 caaagagttg tatatcaagg cacattgatg cctttatcgc cgtcggagca cgtccggcgg
    16921 cgctattcac gctgatctcc tgtgactcga cgcggcagat aatgtagtat ctccggcaat
    16981 attgcccctt tgaaggctgg cgaataagtt gaggaatcag aattaatgag cgatatggca
    17041 gagcgccttg cgctacatga atttacggaa aacgcctact taaactactc catgtacgtg
    17101 atcatggacc gtgcgttgcc gtttattggt gatggtctga aacctgttca gcgccgcatt
    17161 gtgtatgcga tgtctgaact gggcctgaat gccagagcca aatttaaaaa atcggcccgt
    17221 actgtcggtg acgtactggg taaataccat ccgcacggcg atatcgcctg ttatgaagcg
    17281 atggtcctga tggcgcagcc gttctcttac cgttatccgc tggttgatgg tcaggggaac
    17341 tggggcgcgc cggacgatcc gaaatcgttc gcggcaatgc gttacaccga atcccggttg
    17401 tcgaaatatt ccgagctgct attgagcgag ctggggcagg ggacggctga ctgggtgcca
    17461 aacttcgacg gcactttgca ggagccgaaa atgctacctg cccgtctgcc aaacattttg
    17521 cttaacggca ccaccggtat tgccgtcggc atggcgaccg atattccacc gcataacctg
    17581 cgtgaagtag ctcaggcggc aatcgcatta atcgaccagc cgaaaaccac gctcgatcag
    17641 ctgctggata tcgtgcaggg gccggattat ccgactgaag cggaaattat cacttcgcgc
    17701 gccgagattc gtaaaatcta cgagaacgga cgtggttcag tgcgtatgcg cgcggtgtgg
    17761 aagaaagaag atggcgcggt ggttatcagc gcattaccgc atcaggtttc aggtgcgcgc
    17821 gtactggagc aaattgctgc gcaaatgcgc aacaaaaagc tgccgatggt tgacgatctg
    17881 cgcgatgaat ctgaccacga gaacccgacc cgtctggtga ttgtgccgcg ttccaaccgc
    17941 gtggatatgg atcaggtgat gaaccacctc ttcgctacca ccgatctgga aaagagctat
    18001 cgcattaacc tcaatatgat cggtctggat ggtcgtccgg cggtgaaaaa cctgctggaa
    18061 atcctctccg aatggctggt gttccgtcgc gataccgtgc gccgccgact gaactatcgt
    18121 ctggagaaag tcctcaagcg cctgcatatc ctggaaggtt tgctggtggc gtttctcaac
    18181 atcgacgaag tgattgagat cattcgtaac gaagatgaac cgaaaccggc gctgatgtcg
    18241 cggtttggcc ttacggaaac ccaggcggaa gcgatcctcg aactgaaact gcgtcatctt
    18301 gccaaactgg aagagatgaa gattcgcggt gagcagagtg agctggaaaa agagcgcgac
    18361 cagttgcagg gcattttggc ttccgagcgt aaaatgaata acctgctgaa gaaagaactg
    18421 caggcagacg cgcaagccta cggtgacgat cgtcgttcgc cgttgcagga acgcgaagaa
    18481 gcgaaagcta tgagcgagca cgacatgctg ccgtctgaac ctgtcaccat tgtgctgtcg
    18541 cagatgggct gggtacgcag cgctaaaggc catgatatcg acgcgccggg cctgaattat
    18601 aaagcgggtg atagcttcaa agcggcggtg aaaggtaaga gtaaccaacc ggtagtgttt
    18661 gttgattcca ccggtcgtag ctatgccatc gacccgatta cgctgccgtc ggcgcgtggt
    18721 cagggcgaac cactcaccgg caaattaacg ttgccgcctg gggcgaccgt tgaccatatg
    18781 ctgatggaaa gcgacgatca gaaactgctg atggcttccg atgcgggtta cggtttcgtc
    18841 tgcaccttta acgatctggt ggcgcgtaac cgtgcgggta aggctttgat caccttaccg
    18901 gaaaatgccc atgttatgcc gccggtggtg attgaagatg cttccgatat gctgctggca
    18961 atcactcagg caggccgtat gttgatgttc ccggtaagcg atctgccgca gctgtcgaag
    19021 ggcaaaggca acaagattat caacattcca tcggcagaag ccgcacgtgg cgaggatggt
    19081 ctggcgcaac tgtacgttct gccgccgcaa agcacgctga ccattcatgt tgggaaacgc
    19141 aaaattaaac tgcgtccgga agagctacag aaagtcactg gcgaacgtgg acgccgcggt
    19201 acgttgatgc gcggtttgca gcgtatcgat cgtgttgaga tcgactctcc tcgccgtgcc
    19261 agcagcggtg atagcgaaga gtaatatgcc ggggagggcg ctgtttcctc ccgctagcaa
    19321 ggaacgccgg atgaaacgtt aagtcgtcat ccggcagcgt attcaacccc cggaaacgat
    19381 aaaaattccc ttgaaaccgt tgtttattca tgcgttgcga ttaacaatac gcttttccag
    19441 agagcggctt ttaacaatgc cctaacctga tttcaggtga cgtacaatgc cagtctctca
    19501 gaccttcaga gggtgttatg ctatatatct ttcgtcttat tattaccgtg atttacagca
    19561 tcttagtctg tgtattcggc tccatttact gccttttcag cccgcgtaac ccgaaacatg
    19621 tggccacctt tgggcatatg tttggccgtc ttgcgccact gttcggcctg aaagttgagt
    19681 gccgcaaacc tgcagatgct gaaagctacg gcaatgctat ctatatcgct aaccaccaga
    19741 acaactatga catggtgaca gcatcgaaca ttgtgcaacc gccgacggtg acggtaggta
    19801 aaaagagctt gctgtggatc cccttcttcg ggcagttgta ctggttaacc ggcaacttat
    19861 tgatcgacag aaacaatcgc actaaagctc acggcaccat tgcggaagtc gtgaatcatt
    19921 tcaaaaaacg ccgtatttcc atctggatgt tcccggaagg aacccgcagc cgtggtcgcg
    19981 gcctgctacc gttcaagact ggagcatttc acgcggcaat tgcggcgggc gtcccgatta
    20041 ttcccgtgtg cgtctctaca acttcgaata agattaatct taatcgactg cacaacggtc
    20101 tggtgattgt cgaaatgctg ccgccaattg acgtcagtca gtatggcaaa gatcaggttc
    20161 gtgagctggc tgcccattgt cgttcgataa tggaacaaaa aatcgccgag ctcgataaag
    20221 aagtcgcaga acgcgaagcc gccggaaaag tttaagtcgg caatctgtat ttttgcgggg
    20281 aacactttcc tgcacggtat tactttagcc agttttacat ggagcaaata tgtcactcag
    20341 tcggcgtcag ttcattcagg catcggggat tgcactttgt gcaggcgctg ttcccctgaa
    20401 ggccagcgca gccgggcaac agcaaccgct acccgttccg ccgctacttg aatctcgccg
    20461 tgggcaaccg ctgtttatga ctgtacaacg tgcgcactgg tcatttacgc cagggacacg
    20521 cgcgtcggtc tggggaatca atggtcgtta cctggggccg actatccgcg tctggaaggg
    20581 cgacgatgtt aagcttattt acagcaaccg cctgacagaa aatgtctcaa tgacggtggc
    20641 cgggctacag gtaccaggcc cgctgatggg cggtccggca cggatgatgt cgccaaacgc
    20701 tgactgggca cccgtactgc ctattcgcca gaacgcagct actctgtggt atcacgccaa
    20761 tactcccaac cgcacggctc agcaggtcta taacggcctt gccggaatgt ggctggtgga
    20821 agatgaagtc agcaagtcgc tgcctatccc caaccattat ggtgtggatg attttccggt
    20881 cattatccag gataaacggc tggataactt tggtacgcca gaatacaacg aaccgggaag
    20941 cggcggcttt gttggtgata cgctgctggt taacggtgta caaagcccgt acgttgaagt
    21001 ctcgcgtggc tgggtgcgct tgcgactgct gaacgcgtcg aactctcgtc gctatcaact
    21061 acagatgagc gatggtcgcc cgttacatgt gatttctggc gatcagggat tcctgcctgc
    21121 tcctgtatcg gtgaagcaac tttcgctggc accgggcgag cgccgcgaga ttctggtgga
    21181 tatgagcaac ggcgatgaag tgtcgatcac ctgtggcgaa gcggcgagca ttgttgatcg
    21241 tattcgcggc ttctttgagc catccagtat tctggtttct accttggtgc taacgctgcg
    21301 cccaaccggc cttctgccgc tggtcacaga cagtcttccg atgcgcttgc tgccaactga
    21361 aatcatggct ggttcgccaa ttcgcagtcg cgatatcagt ctgggtgatg acccgggtat
    21421 taatggacag ctgtgggacg tcaaccgtat tgatgtcacc gcgcagcaag gaacgtggga
    21481 acgctggacg gtacgcgcgg acgagccgca agcgttccat attgaaggcg taatgttcca
    21541 gatccgtaac gtgaatggcg cgatgccgtt cccggaagac agaggctgga aagataccgt
    21601 ttgggttgac ggacaagtgg agctgcttgt ttatttcggt cagccttcct gggcgcactt
    21661 cccgttctac ttcaacagtc agacgctgga aatggcggac cgtggctcga ttgggcaact
    21721 gttggtcaat ccggtaccgt aatcttcata ctcgcccgag gagggcgagt atggaatacc
    21781 tttcgatttc tctttcgttc cggcttataa tccccggcct ttgattattt ttttatcatt
    21841 tctggaagca aaatgagctc tatctccctg atccaaccgg atcgcgacct gttctcctgg
    21901 ccgcagtact gggccgcctg ttttggaccg gcaccgtttt tgccgatgtc tcgtgaagag
    21961 atggatcaac ttggctggga tagctgcgac atcattttgg ttactggcga cgcgtatgtc
    22021 gatcacccaa gcttcgggat ggcgatttgc ggtcgtatgc tggaagcgca gggctttcgc
    22081 gtcgggatca tcgcccagcc ggactggagc agcaaagacg actttatgcg tctgggtaaa
    22141 ccgaatctgt tttttggtgt tactgctggc aacatggatt cgatgatcaa ccgctatacc
    22201 gccgatcgcc gtttacgtca tgatgatgcc tacacgccag ataatgtcgc gggtaagcga
    22261 ccggatcgcg ccacgctggt ttatacccag cgttgtaaag aggcgtggaa agatgtgccg
    22321 gtgatcctcg gcggtattga ggctagcctg cgccgtaccg cgcattatga ttactggtcc
    22381 gataccgtgc gccgttccgt tctggtagat tcgaaagccg acatgctgat gtttggtaac
    22441 ggtgaacgtc cgctggtgga agtagcgcat cgtctggcga tgggcgagcc gattagtgaa
    22501 atccgcgatg tgcgtaatac cgcaattatc gtaaaagagg cgctgcctgg ctggagcggt
    22561 gtggattcca cccgtcttga taccccaggg aaaatcgacc caatcccgca tccgtatggc
    22621 gaagatttgc cgtgtgcgga taacaaacct gttgcgccga aaaagcagga agcgaaagct
    22681 gtaatcgtgc agccaccgcg ccagaaaccg tgggaaaaaa cctacgtatt gctgccttct
    22741 ttcgagaaag tgaagggcga taaagtgctg tacgcccatg cttcgcgcat tctgcaccac
    22801 gaaaccaacc caggctgcgc ccgcgcattg atgcaaaaac acggtgaccg ctatgtgtgg
    22861 atcaacccgc ctgctatccc gctttctacc gaagagatgg actgcgtttt tgcgctgccg
    22921 tacaagcgcg tgccacatcc ggcctatggc aatgcccgta ttccggctta cgaaatgatc
    22981 cgtttttcgg tcaacattat gcgcggctgc tttggcggct gttctttctg ttctattacc
    23041 gagcacgaag ggcgcattat tcagagccgt tcggaagatt cgatcattaa tgagatcgaa
    23101 gcgatccgcg acaccgttcc aggttttacg ggcgtgattt ccgatctcgg tgggccaact
    23161 gccaacatgt atatgttgcg ctgcaaatcg ccacgcgctg aacaaacttg tcgccgtttg
    23221 tcgtgcgttt atccggatat ttgtccgcac atggacacta accacgaacc gacgatcaac
    23281 ctctatcgcc gtgcgcgtga tctgaaaggc attaaaaaaa tccttatcgc ctcaggcgta
    23341 cgttatgaca tcgccgtgga agatccgcgc tatatcaaag agttggcgac ccatcacgtc
    23401 ggcggttatc tgaagattgc tccggaacat accgaagaag ggccgttgtc gaagatgatg
    23461 aagccgggca tgggcagcta tgaccgcttt aaagagctgt tcgataccta ctcgaaacag
    23521 gcaggtaaag agcagtatct gatcccgtat ttcatctctg cgcaccccgg tacgcgtgat
    23581 gaagatatgg tgaatttggc gctgtggctg aaaaagcatc gtttccgtct cgaccaggta
    23641 cagaacttct acccatcgcc gctggcgaac tcaaccacca tgtattacac cgggaaaaaa
    23701 ccgctggcga agattggtta taagagcgaa gacgtcttcg taccgaaggg cgacaaacag
    23761 cgtcgtttgc ataaagcgtt gttgcgttac cacgatccgg caaactggcc gttaatccgc
    23821 caggcgctgg aagcgatggg caaaaagcat ctgattggca gcccgcgatt gcttagtgcc
    23881 tgcgccaacc attgaagaga tgcgtgaagc tcgtcgccag aaccgcaata cccgtccggc
    23941 gttgactaaa catacgccga tggcgactca gcgtcagacg cctgctacgg caaaaaaagc
    24001 gtcgtctacg caatctcgtc cggtgaatgc tggtgcgaag aaacgcccta aagcggcggt
    24061 tggacgttaa aaaaaatccc cggcaactga catgctgccg gggatttttt tatcattctg
    24121 cgtacatacg caagcagcgc aggcaatcaa aatagtcctg tgcatcaaga cgcgtattgg
    24181 ctctgttagc ttcggatttg gcagcataga tttgtgggcg tagtgctttc atatcctgct
    24241 caagccgggc gattttggca tttaatgttt gtacattgct cgccagttga ttgacctttt
    24301 gctcattgac gcagccactg agcaagcagg ttgccagaac aacagctccc acggtgaaaa
    24361 tcgttttcat atagtgttcc cttttattta ttgttccggc taattgttat ttaattcagc
    24421 tcattgcttt aatctggcgg cggtttttgc ctcaatcagc ttaagtaggc tgccgctgtt
    24481 cgggcggata aaatcagcga tttcccactt accttcgcgc cgctgtaaaa ccagctcttg
    24541 ctcttcattg ttgccattgg tcagcgtaaa acgaacatga atgcgggcgg aatcggcggt
    24601 tttctccagg actcgaattt catcaacgcg tgaaaatagc gactcttcgc caaaacagat
    24661 cggattgtcg taaataaatt gccgttctat ttgcatatct tcatcgggat tattggccgt
    24721 cttgaaggtg gttttataga aggcattaac cgcctggcgg taagagtcgg aaaccaccca
    24781 cgtccacggc cagctataat cagcaatatg ttcaaagagt tcagcaacaa aggattctgg
    24841 ctgttctttc tgcaacgaag ctaaagcggc cagcgttttt tcattctcgc tattaactgc
    24901 ttgtaagacg ctgccatgat tgctgacaat atcatcaatg acccaacgac cattttccgc
    24961 caccattttc agtgtctgtg tggtcttttc tttatcatct ttaaagatac gaaagcgcac
    25021 aacggcatcg gcatgatcga cgtcagtttg ggttatcgct acgctttcta acaccagatc
    25081 gccaaaatcc tgacaatcac aaaccggatc ataatccagc cagccaatat tgcccggtag
    25141 tgtaagattg ttgttcaggg taagcgcctg ttgaatacgc gctgaagtta tcgcccgctc
    25201 tccggtttca ccgaaataag gcgtactggc atctgattta tagttctgat aaatctgccg
    25261 gactgtttgt tctacggtct gtgaaggagg ctgcgcgtgt acggtaaaac ttgccagggc
    25321 tgctaaaaac agaagtatta ttttcatctg tttttcacct ctccgttgcg tttatcccgg
    25381 atatagatga gttgttcttt taagagcttc cggctctgca tgatgatgtc cttatatttg
    25441 gcattcctga acaaggaaga gtaacaacgg gcgggacgcg aggggaataa atgatttctg
    25501 aaaagtccgg tagcggaaca ttaccgccac cgggagaatt tgcacgttag ccgccgaact
    25561 ggtcaggatc gggaccgaga cgcttgccct gatcgagttt tgcaattttg ccgagttcgt
    25621 ctttgtcgag acggaaatcc cagacatcaa agttttcggc aatacgtgaa ggtgtgaccg
    25681 atttcgggat caccaccagg ccgctatcca gatgccagcg gataacaatc tgcgccgggg
    25741 ttttgccgta tttatctgcc agatcgcgaa tgactttctg atcgaaaacg ccttttcctc
    25801 cttgcgctaa tgggctccag gattcggtct ggattttgtg tgtcgcgttc caggcgtgta
    25861 gctggcgttg ttgcatcagc ggatgaagtt cgatctggtt tatcacaggc gtcacgccag
    25921 tttcatcaat caggcgttgc aggtgatgga tctggaagtt gcacacgccg atgcttttga
    25981 ttaatccctc tttttgcaat tcgatcatgc ctttccatgc ttcgacataa tggtcgatag
    26041 cgggaacggg ccagtgcatt aagtagaggt cgatataatc aagctggagt tttttcaggc
    26101 tgtcgagcag ggcttcgcgg gggcacttgt ggtcgtcgtt ccacagctta gtggtgatga
    26161 acagttcttc tctgttgact gaggcatttt tcagggcttt gccgacacct tcttcgttct
    26221 tgtaggccgc ggcggtatca atcgagcgat aacccacttc taacgctttt tgaatggcgg
    26281 tgattacttc ctcattactt gcttgccaga cgcccagtcc cagctggggc atgacattgc
    26341 catcctgtag cttaataacg gttggattag ccatacgttc ctcctttata tgaactcacc
    26401 ggagcaggct ccggtgaggt gttgtgaact taagtctgga cgaaatgccc gaaaacgaaa
    26461 gtttgaggcg taaaaagctt agcgggcggc tttgtatata cggcggctga catccaacgt
    26521 aatgtcatga ttttcgccca gttgggtcat gccgtgctct tccagttttt tcagcaaagc
    26581 cgggatggag ctgccgtcca gaccgtagtc ggagaggtgg gtcggcacgc ctaattgctc
    26641 aaagaaattg cgggttgcgg caatcgcggc gtcaatacgc tcatcgtcgg aaccttcagt
    26701 gatgttccag acgcgttcag catattgcag cagcttagcg cgcttggtct cgcgtttttc
    26761 attccacagt gcaggcagga cgatagccag tgtttgcgcg tgatccagac cgtgcatcgc
    26821 agtcagttcg tggcccagca tatgcgttgc ccagtcctgc ggtacgccag cgccaatcaa
    26881 accgttcagc gcctgagtcg ccgcccacat gacgttggcg cgcacatcgt agttttctgg
    26941 ctctttcagg gctttcggac catcttcgat tagcgtcagc aaaatgcctt ctgcgaaacg
    27001 gtcctgaatt ttggcatcaa ccggtttggt aacatactgt tccacggtgt gtacaaaggc
    27061 gtccactacg ccgttagcca cctgacgcgg cggcagggtg taggtataaa ccggatcgag
    27121 cacggcaaat accggctgaa catgtgcaga atggaacgcc tgcttgtcgc ctgtggtttt
    27181 acgggagatc accgcgcctg cgttggattc tgaaccggtt gctggcagcg tcagcacaca
    27241 gcccatcggg atggcgcttt taatctcttt accgcccgtt tgcagaatgt gccacggatc
    27301 gatattttcc ggatagttag ccgctgcggc gataaatttg gtgccgtcca gtacagaacc
    27361 gccgccaacc gccagcagga aagtcacttt ctgttcgcga accagtttca cggcgttcat
    27421 cagcgtttca taagccgggt ttggctcaat accgccaaat tccagcacgt ccatgccttt
    27481 cagggcatcc agaacttgat cgagaacgcc ggtttttttc acgctgccgc caccgtaggt
    27541 aatcaatacg cgagcatcgt gaggaatttg ttcgcgtaaa ccagcgattg cgcctttacc
    27601 aaacagaatg cgggttgggg tgtgcagatt aaagttgttc attacttgct ccctttgctg
    27661 ggccaatatg agggcagaga acgatctgcc tgatgttttt cattgtgatc gccagcgccc
    27721 tagctctcaa tgctcatttc tgccaatgtc ttgcctattt ctccagagtg ctggagaaat
    27781 gctacaaaat tgcgcacaat caaattgccg cattattcct aagaaattac gcgatatgaa
    27841 acgtgaagag atttgtcgct tgctggcgga taaagttaat aaactgaaaa ataaagaaaa
    27901 tagtttgtca gaactgttgc ccgatgtgcg tttgttgtat ggcgagacgc ctttcgcacg
    27961 tacaccggtg atgtacgagc ctggcatcat aattctcttt tccgggcata aaatcggtta
    28021 tatcaat
//
LOCUS       DAJVQH010000040        27739 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJVQH010000040 DAJVQH010000000
VERSION     DAJVQH010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.040"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..1100)
                     /gene="ycjM"
                     /locus_tag="OQM87_001824"
     CDS             complement(<1..1100)
                     /gene="ycjM"
                     /locus_tag="OQM87_001824"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCS1507983.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OQM87_001825"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OQM87_001825"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCS1507984.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OQM87_001826"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OQM87_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCS1507985.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OQM87_001827"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OQM87_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCS1507986.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OQM87_001828"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OQM87_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCS1507987.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OQM87_001829"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OQM87_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCS1507988.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="OQM87_001830"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="OQM87_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCS1507989.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCATLNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OQM87_001831"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OQM87_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCS1507990.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHSDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OQM87_001832"
     CDS             complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OQM87_001832"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCS1507991.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8574)
                     /gene="puuC"
                     /locus_tag="OQM87_001833"
                     /pseudo
     CDS             complement(7086..8574)
                     /gene="puuC"
                     /locus_tag="OQM87_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="OQM87_001834"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="OQM87_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCS1507992.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNVN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="OQM87_001835"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="OQM87_001835"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCS1507993.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="OQM87_001836"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="OQM87_001836"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCS1507994.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="OQM87_001837"
     CDS             11851..12015
                     /locus_tag="OQM87_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1507995.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="OQM87_001838"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="OQM87_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCS1507996.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="OQM87_001839"
     CDS             13524..13769
                     /locus_tag="OQM87_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCS1507997.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="OQM87_001840"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="OQM87_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCS1507998.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVDVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="OQM87_001841"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="OQM87_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCS1507999.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="OQM87_001842"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="OQM87_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCS1508000.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="OQM87_001843"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="OQM87_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCS1508001.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="OQM87_001844"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="OQM87_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCS1508002.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="OQM87_001845"
     CDS             19432..19785
                     /locus_tag="OQM87_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCS1508003.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="OQM87_001846"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="OQM87_001846"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCS1508004.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21087..22214
                     /locus_tag="OQM87_001847"
     CDS             21087..22214
                     /locus_tag="OQM87_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCS1508005.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRENNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22282..24216
                     /locus_tag="OQM87_001848"
     CDS             22282..24216
                     /locus_tag="OQM87_001848"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCS1508006.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24294..24396)
                     /locus_tag="OQM87_001849"
                     /pseudo
     CDS             complement(24294..24396)
                     /locus_tag="OQM87_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24450..26424
                     /gene="pdeR"
                     /locus_tag="OQM87_001850"
                     /pseudo
     CDS             24450..26424
                     /gene="pdeR"
                     /locus_tag="OQM87_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26572..26745
                     /locus_tag="OQM87_001851"
     CDS             26572..26745
                     /locus_tag="OQM87_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508007.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26835..27584
                     /locus_tag="OQM87_001852"
     CDS             26835..27584
                     /locus_tag="OQM87_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1508008.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         6433 a         7194 c         7125 g         6987 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaatgatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggacgggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccaggtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg atctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa ctgcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaagaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataatttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 gacgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctaataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgccctcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggcgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gagtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgcgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttggcgtcc agcagctcca gctgtttatt gccgtagcgt tcccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtatcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgca cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatccg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgagct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgagg cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttaa cgttatgaac cagcttcatt
     9001 gacaccccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgctccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaagtg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaaccaa atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctggtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacaagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cagagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtacctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttgacgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcgatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagaggtc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcga tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg caactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtaccg ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg cgttatctct gttatacctt
    21001 tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt caggataacg
    21061 atttccttta cgaccaagga gcgcccatgg aacaacgcca catcaccggc aaaagccact
    21121 ggtatcatga aacgcaatcc agtactacgg agtatgacgt tctgcctctg gtcccggaag
    21181 ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct tgatgaagag acgctggccc
    21241 ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt ggagttgttc cctgaccagc
    21301 ttaccgtgac ccgttcacaa accttcaccg cttatgaacg cttgtcgacg gccctgacgg
    21361 ttgctcaggt ttgcggcgtc cagcggttat gtaactacta ttcggcgcga cttacgccgc
    21421 tccccggacc tgattcctcc agggaaaata atcatcggtt ggcacaaatc acgcaatatg
    21481 cccgccaact ggctagctcg cctgctatta tcgacaaccg atcgcgccag catctgaatg
    21541 acgtcggtct tactgtctgg gactgtgtga tcattaacca aatcattggt tttattggct
    21601 ttcaggcgcg gacaattgcg acatttcagg cttatctcgg gcatccggta cgctggttac
    21661 ccgggctggc gatacaaaac tacgccgacg cgtcactgtt tgctgatgaa tcaatacgct
    21721 ggcgaagcag ctatgaggtg gaaaaactac ctgaagagca cacaaaaagt tcaactgcag
    21781 aactttgcca actggccgaa acacactctc tccaccctat ttcactttcc cttctcgaaa
    21841 agttgttaaa cagcacacgg gtcaatacac agccggataa tcagcttgcg gcgttgttat
    21901 gcgcgcgtat aaatggcagt cctgcttgtt tttccacctg tatggattca tcaaatgaat
    21961 ataaaaaaat cagcaccctt ctgcgcaagg gcgaaaatga aattaaccga tgggctgacc
    22021 gtcattctgt tgagcgcgct accgttcagg cgatacaatg gctgacccga gcacccgatc
    22081 gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca acgcagatta
    22141 ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg
    22201 gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca gccgctctaa
    22261 tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca gcttaaacag
    22321 caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga aaaaggcttt
    22381 ggcttcctgg aagtcgacgc acaaaaaagt tatttcattc cgccgccgca gatgaaaaaa
    22441 gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg tgaatccgca
    22501 gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt tcagggcaaa
    22561 aatgaccgtc tgaccatcgt tcctgatcat ccacttttaa aagacgccat tccttgccgc
    22621 gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc cgaaatgcgc
    22681 cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata catcactttt
    22741 ggcgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct ggaaaaagaa
    22801 gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga agatctgacc
    22861 tcgctggatt ttgtcaccat cgacagtgcc agcactgaag atatggatga cgcccttttc
    22921 gctaaggcgc tgccggatga caaacttcag ctgattgtgg cgattgccga tccaaccgcg
    22981 tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat
    23041 ctgcctggct tcaacatccc tatgctgcct cgcgagcttt ctgacgatct ctgctcactg
    23101 cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc tgatggcacc
    23161 attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa gctggtgtat
    23221 gaccaggttt ctgactggct ggaaaatacc ggtgactgga agcctgaaag tgaagcaatt
    23281 gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac
    23341 cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga aaaaggtgaa
    23401 gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg
    23461 attgccgcta acatttgtgc ggcccgcgta ctgcgcgata agctcggttt tggcatctat
    23521 aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt gctgaaaacg
    23581 cacggtctgc atgtcgatgc cgaagaagta ctcacgctgg acggtttctg caaactgcgt
    23641 cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg cttccagtca
    23701 tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga agcatacgcc
    23761 acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct gctgaaagcg
    23821 gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca aatggccgaa
    23881 cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata cgcacgcttc
    23941 ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga tatcagccgt
    24001 ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc ggcacctttc
    24061 ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt acaaattaaa
    24121 ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga agtccgcatg
    24181 gaaacccgca gcattattgc gcgcccggtc gcgtaatctc ctttcacggc ccattcctca
    24241 tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat tttttaactg
    24301 actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata tataaaaaac
    24361 aatgcgttta tccgccgtta atttcatgta agtcatgttg ttccttttat ttaaccgcgg
    24421 actccgctgt taaccggagg atatgcatca tgaaaaccgt tagggagtcc acaacgttgt
    24481 acaactttct cggatcgcac aatccatact ggcggttgac ggaaagcagc gatgctttgc
    24541 gcttttctac caccaaaacc acagaacctg atcgtacatt gcagttatct gccgaacagg
    24601 ctgctcgcat cagggaaatg acggtcatca cctccagcct gatgatgagt ctgaccgtcg
    24661 atgaaagcga tctttctgtg catctggtag gacgaaaaat caataaacgg gaatgggctg
    24721 gcaacgcgtc tgcatggcat gacacaccgg cggttgctcg tgatttatca cacgggcttt
    24781 cctttgctga gcaggtagtt tctgaagcac attccgcaat agtgattctc gacagccggg
    24841 ggaatatcca acgcttcaat cggttatgtg aagattacac agggttgaaa gaacacgacg
    24901 tcattgggca aagcgtgttt aaactgttta tgagccgtcg tgaagctgcg gcatccaggc
    24961 gcaataaccg tgtatttttt cgaagcggca atgcatatga agtcgaactg tggataccaa
    25021 cacgtaaagg ccagcggctg tttctgtttc gcaataaatt tgtccacagc ggcagtggca
    25081 aaaacgagat ttttttaatc tgttccggta ccgacattac cgaagagcgc cgcactcagg
    25141 agcgactgcg tattctggca aataccgaca gtatcaccgg actgccgaat cgtaacgcaa
    25201 tgcaggagtt aatcgatcac gctattaatc aggcagataa caataaagtt ggggttgtgt
    25261 atcttgattt ggataatttt aaaaaggtca acgacgccta tgggcatttg tttggtgacc
    25321 agttattacg cgacgtgtca ttggctattt taagctgtct cgaacatgac caggtgttgg
    25381 cgcgtccagg tggcgatgag tttctggtac tggcatccaa tacctcacaa agcgcgctgg
    25441 aagcaatggc atcacgaatt ttgacccgct tacgcctccc ctttcgtatt ggtttaattg
    25501 aagtttatac cagctgttca gtaggtattt cgctctctcc cgaacatggt tcggacagcg
    25561 cgactattat tcgtcacgcc gacacagcaa tgtacacagc gaaggaaggc ggacgaggac
    25621 aattttgcgt ttttactcca gaaatgaatc agcgggtatt tgaatatctc tggctggata
    25681 ccaacttgcg taaagcactg gaaaacgatc agttggttat cgaaaatcac ctagcgtggc
    25741 gaagtgcgca gtctggaagc actagtacgt tggcagtcac ctgaacgtgg gttgattcca
    25801 ccgttggact tcatttccta cgccgaagag tcagggctaa ttgtgccttt aggccgttgg
    25861 gtgattctcg atgtcgtacg ccaggtggca aagtggcggg ataaaggcat aaacctgcga
    25921 gtggcggtaa atatttctgc acgtcagctc gccgatcaaa ccattttcac cgccctgaaa
    25981 caggttctcc aggaactcaa ttttgaatac tgccctatag atgttgaact gacagagagt
    26041 tgtctgattg agaatgatga actggcactg tctgttattc aacaatttag ccaactaggt
    26101 gcgcaagtgc atctggatga ttttggtacc ggctactctt cactttcgca actggcgcgc
    26161 tttccgatcg atgccatcaa acttgaccag gtttttgttc gagatattca caaacaacct
    26221 gtctcgcagt cactggtccg ggcgatcgtc gctgtggccc aggcattgaa tcttcaggtg
    26281 atcgccgaag gtgtagagag tgcaaaggaa gatgcttttt taaccaagaa cgggatcaat
    26341 gagcggcaag gatttttgtt tgccaaaccg atgcccgccg tcgccttcga acgctggtat
    26401 aaacgctatc tgaagcgcgc ataagtagat gaatatatat acttagcgct atatcccgtc
    26461 attatatatc gtattcctgg atgataatta gccgttgctg ttaaacactt ctcttttagc
    26521 ggcaattttt gcattattca ttttccccct tttgctataa ggatgtcctt gatgtctgaa
    26581 tttgatgccc agcgcgttgc cgaacgtatt gatattgtgt tagatatcct tgtggcaggc
    26641 gattaccact ctgcgatcca taatctcgag atacttaaag cagaactctt gcgccaggtt
    26701 gctgaatcta cgccagacat cccgaagaca ccgtgggaga tttaacaatc gtgccttcag
    26761 gttttcctat ttgaggatgc ccggcgcgtc agtatttttt tatttagtat tataacgtta
    26821 taagaattac agcgatgaat tcccgacaac aaactattct acagatggtc attgaccagg
    26881 gtcaggttag cgtaaccgat ctggcaaaag ccactggagt ttctgaagtt accattcgcc
    26941 aggatctcaa caccctcgaa aaactgagtt acctccgccg tgcacatggc tttgcagttt
    27001 cgcttgatag tgatgacgtc gaaacccgta tgatgagcaa ctatacgctg aagcgtgaac
    27061 tcgccgagtt tgccgcgtca ctggttcaac cgggcgaaac catctttatc gaaaatggca
    27121 gcagcaatgc cctgctcgcc cggactctgg gggagcagaa gaaaaatgtc actatcatca
    27181 cggtcagcag ctacatcgcg catttgctga aagacgcgcc ttgtgaagtc attttgctcg
    27241 gtggcgtgta ccagaaaaaa agcgaaagta tggttggccc tttgacacgc cagtgcatcc
    27301 agcaggtaca tttcagcaaa gcatttattg gtattgatgg ctggcaacct gaaactggat
    27361 tcaccggtcg cgatatgatg cgcaccgatg tggtcaatgc cgtgctggaa aaagagtgcg
    27421 aagcgatagt cctgactgac agctcgaaat ttggtgctgt acattcatac tccatcggtc
    27481 ccgttgagcg attcaatcgc gtgattaccg attcgaaaat acgcgccagc gatctgatgc
    27541 atcttgagca cagtaaactc accgttcacg tcgttgacat ttaaccattt cccttctcta
    27601 cggatgattt gcagtttggc aaatcatccg ctctaagatg attcctggtt gataattaag
    27661 actatttacc tgttattaac actctcaaga tataaaatta ttatcagcga tataacagga
    27721 agtaattatc acgtgcgtg
//
LOCUS       DAJVQH010000041        27313 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJVQH010000041 DAJVQH010000000
VERSION     DAJVQH010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.041"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OQM87_001853"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OQM87_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCS1508009.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OQM87_001854"
     CDS             complement(1614..1937)
                     /locus_tag="OQM87_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCS1508010.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OQM87_001855"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OQM87_001855"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCS1508011.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OQM87_001856"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OQM87_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCS1508012.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLHDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OQM87_001857"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OQM87_001857"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCS1508013.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OQM87_001858"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OQM87_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCS1508014.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OQM87_001859"
     CDS             7537..8574
                     /locus_tag="OQM87_001859"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1508015.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OQM87_001860"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OQM87_001860"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCS1508016.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OQM87_001861"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OQM87_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCS1508017.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OQM87_001862"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OQM87_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCS1508018.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OQM87_001863"
     CDS             10745..11626
                     /locus_tag="OQM87_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1508019.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OQM87_001864"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OQM87_001864"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCS1508020.1"
                     /translation="MTVTTFSELELDKSLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OQM87_001865"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OQM87_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCS1508021.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OQM87_001866"
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OQM87_001866"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCS1508022.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OQM87_001867"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OQM87_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCS1508023.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OQM87_001868"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OQM87_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCS1508024.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OQM87_001869"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OQM87_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCS1508025.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OQM87_001870"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OQM87_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCS1508026.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTLTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OQM87_001871"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OQM87_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCS1508027.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OQM87_001872"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OQM87_001872"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCS1508028.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OQM87_001873"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OQM87_001873"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCS1508029.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OQM87_001874"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OQM87_001874"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCS1508030.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OQM87_001875"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OQM87_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCS1508031.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OQM87_001876"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OQM87_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCS1508032.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OQM87_001877"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OQM87_001877"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCS1508033.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OQM87_001878"
     CDS             24893..25273
                     /locus_tag="OQM87_001878"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCS1508034.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OQM87_001879"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OQM87_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCS1508035.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OQM87_001880"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OQM87_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCS1508036.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..>27313
                     /locus_tag="OQM87_001881"
     CDS             26442..>27313
                     /locus_tag="OQM87_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCS1508037.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYP"
BASE COUNT         6732 a         6805 c         7158 g         6618 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tatttaccga aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgtgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattaa aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggcttttg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccaccg caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccattgc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggcgatcgca
    15241 aataccagcc ccggaaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatctact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatgc tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagttcc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgctaac ttggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgaggg cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtacgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcttg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggtta tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatac aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac gactatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cattaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttcacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggagtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgctatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgacct caaagccaaa gtagcccggc agaagctgag cccggacagt ttcacaacct
    26881 acggggtgcg tatcgaccgg attaaagcaa cgttcggcga gcgtgaggtg ttcagcgacg
    26941 taacatacaa tcggctggtg gaagtgctgg acgagtggat cgccactcgc tcgaacaata
    27001 acgccctgga gttgtttgcc gagctccgtc ggttctggaa gttctgcgca cctactcttt
    27061 gcaacggccg caatgttgcc gccagtctgc cagatgatta tgtttccttc cgcgtacaga
    27121 aacctacccc cacacggctt tttaccgata ttgaatcaat cgcccgactc tggctcaatg
    27181 ttgctgcctg cacctctgta caccagaaga atgctgttcg cttcatgatc atcactggtg
    27241 ttcgtccgat taatgtccat aacctgcgct gggactacgt ttacgaggag gctggtgaaa
    27301 ttgtttatcc gga
//
LOCUS       DAJVQH010000042        27141 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJVQH010000042 DAJVQH010000000
VERSION     DAJVQH010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.042"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..437
                     /locus_tag="OQM87_001882"
     CDS             <1..437
                     /locus_tag="OQM87_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCS1508038.1"
                     /translation="IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQV
                     ELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVETLAWF
                     TVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="OQM87_001883"
     CDS             complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="OQM87_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCS1508039.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1230..2252
                     /gene="pldB"
                     /locus_tag="OQM87_001884"
     CDS             1230..2252
                     /gene="pldB"
                     /locus_tag="OQM87_001884"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCS1508040.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2260..3060
                     /gene="yigL"
                     /locus_tag="OQM87_001885"
     CDS             2260..3060
                     /gene="yigL"
                     /locus_tag="OQM87_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCS1508041.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            3136..4035
                     /gene="bioP"
                     /locus_tag="OQM87_001886"
     CDS             3136..4035
                     /gene="bioP"
                     /locus_tag="OQM87_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCS1508042.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3923..4876)
                     /gene="metR"
                     /locus_tag="OQM87_001887"
     CDS             complement(3923..4876)
                     /gene="metR"
                     /locus_tag="OQM87_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCS1508043.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            5112..7373
                     /gene="metE"
                     /locus_tag="OQM87_001888"
     CDS             5112..7373
                     /gene="metE"
                     /locus_tag="OQM87_001888"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCS1508044.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(7412..8227)
                     /locus_tag="OQM87_001889"
     CDS             complement(7412..8227)
                     /locus_tag="OQM87_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1508045.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            8489..9250
                     /gene="udp"
                     /locus_tag="OQM87_001890"
     CDS             8489..9250
                     /gene="udp"
                     /locus_tag="OQM87_001890"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCS1508046.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            9391..10818
                     /gene="rmuC"
                     /locus_tag="OQM87_001891"
     CDS             9391..10818
                     /gene="rmuC"
                     /locus_tag="OQM87_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCS1508047.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFCRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSC"
     gene            10913..11668
                     /gene="ubiE"
                     /locus_tag="OQM87_001892"
     CDS             10913..11668
                     /gene="ubiE"
                     /locus_tag="OQM87_001892"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCS1508048.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            11682..12287
                     /gene="ubiJ"
                     /locus_tag="OQM87_001893"
     CDS             11682..12287
                     /gene="ubiJ"
                     /locus_tag="OQM87_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCS1508049.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGIRKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            12284..13924
                     /gene="ubiB"
                     /locus_tag="OQM87_001894"
     CDS             12284..13924
                     /gene="ubiB"
                     /locus_tag="OQM87_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCS1508050.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            14003..14272
                     /gene="tatA"
                     /locus_tag="OQM87_001895"
     CDS             14003..14272
                     /gene="tatA"
                     /locus_tag="OQM87_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCS1508051.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTISDKQADTNQEQAKTEDAKRHDKEQV"
     gene            14276..14791
                     /gene="tatB"
                     /locus_tag="OQM87_001896"
     CDS             14276..14791
                     /gene="tatB"
                     /locus_tag="OQM87_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCS1508052.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            14794..15570
                     /gene="tatC"
                     /locus_tag="OQM87_001897"
     CDS             14794..15570
                     /gene="tatC"
                     /locus_tag="OQM87_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCS1508053.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            15612..16394
                     /gene="tatD"
                     /locus_tag="OQM87_001898"
     CDS             15612..16394
                     /gene="tatD"
                     /locus_tag="OQM87_001898"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCS1508054.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="OQM87_001899"
     CDS             complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="OQM87_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCS1508055.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            17046..18539
                     /gene="ubiD"
                     /locus_tag="OQM87_001900"
     CDS             17046..18539
                     /gene="ubiD"
                     /locus_tag="OQM87_001900"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCS1508056.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            18585..19286
                     /gene="fre"
                     /locus_tag="OQM87_001901"
     CDS             18585..19286
                     /gene="fre"
                     /locus_tag="OQM87_001901"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCS1508057.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="OQM87_001902"
     CDS             complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="OQM87_001902"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCS1508058.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="OQM87_001903"
     CDS             complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="OQM87_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCS1508059.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            22931..24262
                     /gene="pepQ"
                     /locus_tag="OQM87_001904"
     CDS             22931..24262
                     /gene="pepQ"
                     /locus_tag="OQM87_001904"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCS1508060.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            24262..24876
                     /locus_tag="OQM87_001905"
     CDS             24262..24876
                     /locus_tag="OQM87_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCS1508061.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            24915..26366
                     /gene="trkH"
                     /locus_tag="OQM87_001906"
     CDS             24915..26366
                     /gene="trkH"
                     /locus_tag="OQM87_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCS1508062.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            26378..26923
                     /gene="hemG"
                     /locus_tag="OQM87_001907"
     CDS             26378..26923
                     /gene="hemG"
                     /locus_tag="OQM87_001907"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCS1508063.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6233 a         6909 c         7474 g         6525 t
ORIGIN      
        1 tgattatcga aaaaatggcc tggctgcata cgctgattat ggtgggcggt ggcctgtatc
       61 tctgctggat gggttaccag atgctacgcg gtgcactgaa aaaagaggct gtatctgcgc
      121 ctgcaccaca ggttgagctg gcgaaaagcg ggcgcagttt cctgaaaggc ttactgacca
      181 atctcgccaa tccgaaagcg attatctatt ttggttcggt attctcattg tttgtcggcg
      241 ataacgttgg cactaccgag cgctggggca ttttcgcgtt gattattgtc gaaacactgg
      301 cgtggtttac cgtcgttgcc agtctgtttg ccctgccgca aatgcgccgt ggttatcaac
      361 gtctggcgaa gtggattgat ggttttgccg gggcgttatt tgccggattt ggcattcatt
      421 tgattatttc gcggtgatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc
      481 cgcatccgac attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat
      541 aaacaacgag ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag
      601 agcaatcctt tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt
      661 ggtgacgccg agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg
      721 aaatagcgcc gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg
      781 ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat ttaagatcaa ttgcgccagc
      841 ggcgcgccac tgctggattc ccagccaaat caagtaagcc gcgcctgccc actttaacac
      901 ttcaaacgca atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat
      961 atgaatcgcc agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg
     1021 gttgagcgag gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat
     1081 cgatgtcagc aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta
     1141 tttttgtgcc acaatacgct actgtcgcag cgttgtgtca ggcacgctta aaaaaacgat
     1201 tttacgtggt ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa
     1261 gagaaaacgc gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg
     1321 atgaagcaga gtttactggt gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac
     1381 agcaccatga ccgggtggta gtcatctgcc cggggcgtat tgagagctac gtaaaatatg
     1441 cggaactggc ctacgacctg ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg
     1501 ggcagggacg ttccggtcgc ctgttagccg atccgcatct cgggcatgtt aatcgcttta
     1561 atgattatgt tgatgatctg gcggcattct ggcagcagga ggttcagccc ggtccgtggc
     1621 gtaaacgcta tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac
     1681 gccatccagg tgtatgtgac gccattgcgc taactgcgcc aatgtttggg atcgtgattc
     1741 gtatgccgtc atttatggca cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc
     1801 gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac
     1861 tgacccacag cagacagcga tatcgacgta acttacgctt ctatgctgat gacccaacga
     1921 ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg
     1981 tgttagccgg tgcgggtgat gacgccacgc caacgcttct cttacaagct gaagaggaac
     2041 gcgtggtgga taaccgcatg cacgaccgtt tttgtgaact ccgcaccgcc gcgggccatc
     2101 ctgtcgaagg aggacggccg ttggtaataa aaggtgctta ccatgagatc ctttttgaaa
     2161 aggacgcaat gcgctcagtc gcgctccacg ccatcgttga ttttttcaac aggcataact
     2221 cacccagcgg aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct
     2281 gatttagatg gcacgttact ttctcccgac catacgttat ccccttacgc caaagaaact
     2341 ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt
     2401 gatgtggggc aaattcgcga taatctggag attaagtctt acatgattac ctccaatggt
     2461 gcgcgcgttc acgatctgga tggcaatctg atttttgctc ataacctgga tcgcgacatt
     2521 gccagcgatc tgtttggcgt agtcaacgac aatccggaca tcattactaa cgtttatcgc
     2581 gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg
     2641 gtgttcaaat atgcgctgta tgagccggga ttactggagc cggaaggcgt cagcaaagtg
     2701 tttttcacct gcgattccca tgaacaactg ctgccgctgg agcaggcgat taacgctcgc
     2761 tggggcgatc gcgtcaacgt cagtttctct accttaacct gtctggaagt gatggcgggc
     2821 ggcgtgtcga aaggccatgc gctggaagca gtggcgaaga aactgggcta cagcctgaag
     2881 gattgtattg cgtttggtga cgggatgaac gacgccgaaa tgctgtcgat ggcggggaaa
     2941 ggctgcatta tgggcagtgc gcaccagcgt ctgaaagatc tgcatccgga gctggaagtt
     3001 attggtacta atgccgacga cgcggtgccg cattatctgc gtaaactcta tttatcgtaa
     3061 tcgttcttta tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc
     3121 aaagagtttt ccattgtggc gctacttatc atcaccacga ttctgtgggc cttctccttt
     3181 agcttttatg gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc
     3241 gttggcttgg cggcactcgt ttttctgccg tttctgcgta cccgtagcaa tagcctgaaa
     3301 acggtcggcc tgtatatgct ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt
     3361 ttccgcgctt atctctacct gacggtctcc gaattgttgc tgttcaccgt actgacgccg
     3421 ctctacatca cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggctatgct
     3481 tttagcgcct tgctggcggt gattggtgcc gggattattc gctatgatca ggtcaccgac
     3541 catttctgga ctggcttgtt gctggtgcaa ctctccaata tcacttttgc catcggcatg
     3601 gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg
     3661 ttctatcttg gcgcgttcct ggtggcggta attgcatggt tcttgctggg aaatgcgcag
     3721 aaaatgccgc aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc
     3781 gggattggct actttatgtg gaactacggc gcgacgcagg tggacgccgg aacgctgggc
     3841 attatgaata atatgcacgt tccggcaggg ctgctggtaa acctggctat ctggcaccaa
     3901 cagccgcact ggccaacgtt tattacaggc gcgctggtga tcctggcctc actgtgggtg
     3961 catcgtaagt gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc
     4021 gcgctgagcg aataaacgct tccgtaactg gctgacgctg ctcgccatcg cgcacggcgg
     4081 cgtacagtcg gctccacaag ccttcgccca gggttttggt caccaccaga ccctggcgct
     4141 caaaactctc tactacccaa tgcggtagcg cggcaatacc catccgcgcg gcaaccattt
     4201 gaatcaataa taaggtgtta tcgacgcttt tcagtgacgg gctgacgcct gccggctgaa
     4261 gaaaatgccg ccagacatcc agtcgactac gctgcaccgg ataaattaat agcgtctcgc
     4321 tggcgagatc ttccggtgta atacgcgttt ttgccgccag tggatggtca ggtgctaaca
     4381 ccaggcgtac ttcataatcg aacatcggcg aataatgcag gccactgcgc ggcaggatat
     4441 cggaggtcat caccagatcc agctcgccct gttgcaacgc gggctgcggg tcaaacgtca
     4501 cgcccgattt aaaatccatc tctacctgcg gccagttctt atggaaattt tctaaagcgg
     4561 gtgtcagcca ctgaatacag ctatggcact caatggcaat gcgcagacgc gtctgctgcg
     4621 gttcattgca ggcttgcagt gcctggctga tttgcggcag cacctggttt gccagttgca
     4681 acaggatttc tccctgcggt gtaaagcgta gcggctggct cttacgcaca aatagccgga
     4741 agccaaggcg ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtctgatgca
     4801 acgttgccgc agcggctgcg agcgagccgc agttccgcaa cgcttgtagc gttttcaggt
     4861 gttttacttc gatcatgaaa gtccttcact tcggcatgaa taatttgcgc ttgaggaata
     4921 tacagtaacc gccaattatg gatgtgtaaa catctggacg gctaaaatcc ttcgtctttt
     4981 aaatttatgg tgcgttggct gcgtttctcc accccggtca cttacttcag taagctcccg
     5041 gcgatgaata aacttgccgc cttccctaaa ttcaaaatcc ataggattta catataatta
     5101 gaggaagaaa aatgacaata ttgaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc
     5161 gcgagctgaa aaaagtgcaa gaaagttatt gggcggggaa ctccacgcgt gaagaactgc
     5221 tgacagtagg gcgtgaactg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg
     5281 acctgctgcc ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtttgctgc
     5341 tgggtaatgt tccgccacgt catcagaaca aagacggttc ggtagatatc gacaccctgt
     5401 tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca
     5461 aatggtttaa caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca
     5521 aactgacctg gacgcagctg ctggaggaag tggacgaggc gctggcgctg agccacaacg
     5581 ttaagcctgt gctgctgggg ccggttactt acctgtggct ggggaaagtg aaaggtgaac
     5641 agtttgaccg ccttagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag
     5701 aactggcgaa acgcggcatc gagtgggtac agattgatga acccgcgctg gtactggaac
     5761 taccacaggc gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga
     5821 aactgctgct gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc
     5881 tgcctgttca ggggctgcat gtggaccttg tacatggtaa agatgacgtt gttgaactgc
     5941 acaagcgcct gccttctgac tggctgctgt ctgcgggtct aatcaatggt cgtaacgtct
     6001 ggcgcgccga tcttactgag aaatatgcgc aaattaagga cattgtcggc aaacgtgatt
     6061 tgtgggtggc atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc
     6121 gtcttgatgc agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactgg
     6181 cactgctgcg cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc
     6241 caattcaggc gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg
     6301 cggcgatcac cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc
     6361 agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa
     6421 ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaacaact
     6481 accgcacggg cattgcggaa catatcaagc aggccattgt tgagcaggaa cgtttgggac
     6541 tggatgtgct ggtacatggc gaggccgagc gtaatgacat ggtggaatac tttggcgagc
     6601 acctcgacgg atttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg
     6661 tgaagccacc gattgtcatt ggtgacatta gccgcccggc accgattacc gtggagtggg
     6721 cgaagtatgc gcaatcgctg accgacaaac cggtgaaagg gatgctgacg gggccggtga
     6781 ccatactctg ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaga
     6841 ttgcgctggc gctgcgtgat gaagtggccg atctggaagc cgctggaatt ggcatcatcc
     6901 agattgacga accggcgctg cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcgt
     6961 atctccagtg gggcgtagag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac
     7021 aaatccacac tcacatgtgt tattgcgagt tcaacgacat catggattcg attgcggcgc
     7081 tggacgcaga cgtcatcacc atcgaaacct cgcgttccga catggagttg ctggagtcgt
     7141 ttgaagagtt tgattatcca aatgaaatcg gtcctggcgt ctatgacatt cactcgccaa
     7201 acgtaccgag cgtggaatgg attgaagcct tgctgaagaa agcggcaaaa cgcattccgg
     7261 cagagcgcct gtgggtcaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc
     7321 gcgcggcact ggcgaacatg gtgcaggcgg cgcagaattt gcgtcggggg taaatccaaa
     7381 ccgggtggta ataccacccg gtcttttctc attacagcga cttcttccca ccatactgct
     7441 taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc
     7501 gataatcagc gttgaatgca tgcccggcat ccgggtacac gataatctct gctttcgcat
     7561 tagcagcccg cagcgcctgg cgcatcgttt caacgctctc ctgcggaatg ctgttatcct
     7621 gaccaccata taagccgaga accggtgcgt taagatcggt tgcgatatca acaggttgtt
     7681 tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta ccacgccact gcggctttta
     7741 gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga
     7801 tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat
     7861 cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac gtggggatat
     7921 cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc
     7981 cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct
     8041 gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag
     8101 gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg
     8161 tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat tgttgtgttg
     8221 ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca
     8281 tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag
     8341 tgatttatgt gatatgaatc acttttggtg ggtaaattta tgcaacgcat ttgcgtcatg
     8401 gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg
     8461 actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct
     8521 cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt
     8581 ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac
     8641 tacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg
     8701 cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg
     8761 tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac
     8821 ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt
     8881 cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatctattg gcgcgacaac
     8941 tcacgttggc gtgacagttt cttctgatac cttctaccca ggtcaggaac gttacgatac
     9001 ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg
     9061 cgtaatgaac tatgaaatgg aatctgcaac tctgctgacc atgtgtgcaa gtcagggcct
     9121 gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc
     9181 tgagacgatg aaacaaactg aaagccatgc ggtgaaaatc gtggtggaag cggcgcgtcg
     9241 tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc
     9301 gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctattttg
     9361 tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct caatcatggt ttacgcagtt
     9421 attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa
     9481 aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa
     9541 caaattaccc aaagcgagca ctggcgtgca gagtgtgaat tgctcaataa cgaagtgcgc
     9601 agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg
     9661 gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc
     9721 ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt
     9781 gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac
     9841 ggtttctgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg
     9901 acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga agcgatcaac
     9961 ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg
    10021 acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc
    10081 atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcggct gccgcaggga
    10141 aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta ttttaacgcc
    10201 gaagacgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat
    10261 atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac
    10321 gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag
    10381 ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg
    10441 gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc aaagccgtaa cgcccagcaa
    10501 atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc
    10561 gcgattggtc aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc
    10621 tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa
    10681 attaaacgcg agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat
    10741 cgacttcggt cggttccggg gcagccgaat gatgaagctt atcaacgcga tgatgaatat
    10801 aatcagcagt cgtgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg
    10861 acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga
    10921 taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga
    10981 tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat
    11041 gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg
    11101 tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg
    11161 cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat
    11221 gggacgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa
    11281 cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct
    11341 gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg
    11401 cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta
    11461 tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga
    11521 cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc
    11581 catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt
    11641 ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag
    11701 tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa
    11761 cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt
    11821 cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg
    11881 ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc
    11941 ttaccgcact gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa
    12001 acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata
    12061 ccggtgatat cgccgctgaa ggaatcagaa aagccatgcg cggaggcgca aagttcctgc
    12121 atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta
    12181 tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg
    12241 ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc ctggtgaagt
    12301 acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc
    12361 caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa
    12421 tcggcataaa gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc
    12481 ggtttggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat
    12541 tgccgatcag ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa
    12601 gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga
    12661 aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa
    12721 tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga
    12781 tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg
    12841 tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt
    12901 gctgcgggaa tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct
    12961 ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat
    13021 ttacggcatt ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt
    13081 gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca
    13141 tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata
    13201 tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc
    13261 agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga
    13321 ttctggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt
    13381 ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa
    13441 tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca
    13501 gaaaaccctg ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg
    13561 gaaaacagcg aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgccct
    13621 ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga
    13681 actggtttac gacagtttgc gccagggcaa gtatttacag cacagtgttg ataagattgc
    13741 ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg
    13801 cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat
    13861 gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg
    13921 ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat
    13981 catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc
    14041 cgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg
    14101 tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac
    14161 cagtcaggat gctgatttta ctgcgaaaac tatctccgat aagcaggcgg atacgaatca
    14221 ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt
    14281 tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc
    14341 gcaacgactg cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact
    14401 ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag
    14461 tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat
    14521 ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga
    14581 aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc
    14641 gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc
    14701 agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gctgaaccga aaaccgctgc
    14761 accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt
    14821 atcacgcatc tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg
    14881 atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg
    14941 atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt
    15001 acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat
    15061 caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg
    15121 ctgctggttt ccagctctct gctgttttat atcggcatgg cattcgccta ctttgtggtc
    15181 tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtgca ggtatccacc
    15241 gacatcgcca gctatttaag cttcgttatg gcgctgttta tggcgtttgg tgtctccttt
    15301 gaagtgccgg tggcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta
    15361 cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgtt gctgacgccg
    15421 ccggatgtct tctcgcaaac gctattggcg atcccgatgt actgtctgtt tgaaatcggt
    15481 gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct
    15541 gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata
    15601 tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc
    15661 gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca
    15721 ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt
    15781 caacggcggg cgttcatcct cacgacagca gccagtggca agctgcgact gaagaagcga
    15841 ttattgagct ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
    15901 accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg
    15961 ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
    16021 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
    16081 cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg
    16141 tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga
    16201 aattgctgat cgaaacagat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat
    16261 catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc
    16321 gtggagaaga tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg
    16381 ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat
    16441 taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct
    16501 gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa gggggggccg
    16561 gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta
    16621 tcgctggcga cgcgccaaag cgcacaaagt ggctgacgcc acgcgtagcg ctgatggtcg
    16681 tggtatgaat cacttctgga tcaaattcca caaacaggta gttagggaat aacggttcac
    16741 tgactgcggt acgtttaccc cgtacgattt tttccagggt gatcatcgga gcgaggcaat
    16801 taaccgcctg tctctcaagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
    16861 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
    16921 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
    16981 atttaacaaa tttacagcat tgcaaagatg aacgccgtat aatgggcgca gattaagagg
    17041 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cctgacgctg cttgaacagc
    17101 agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg
    17161 ctgaccgcac tctgcgtgct ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
    17221 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc
    17281 aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg
    17341 agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag caggtgttaa
    17401 acatgccgac aaagcgactg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg
    17461 acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga
    17521 ttacctgggg gctgaccgta acgcgcggcc cgcataaaga gcggcagaat ctgggcattt
    17581 atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg
    17641 gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    17701 tggcgctggg tgccgatcct gccacgattc tcggtgcagt cacccccgtt ccggatacgc
    17761 tttcagagta tgcgtttgcc ggattgctac gcggcaccaa aaccgaagta gtaaagtgta
    17821 tttccaatga cctcgaagtg ccagccagtg ccgaaatcgt cctggaaggg tatatcgatc
    17881 ctggtgagat ggcgccggaa gggccgtatg gtgaccacac aggttactat aatgaagtcg
    17941 atagtttccc ggtgtttacc gtgacgcata ttacccagcg tgaagatgcg atttaccatt
    18001 ccacctatac cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg ttgaacgaag
    18061 tgtttgtacc gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg
    18121 aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga
    18181 agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga
    18241 tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca
    18301 cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg
    18361 attttgcctc gcctgtctct gggctgggtt caaaaatggg gctggatgcc acgaataaat
    18421 ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtca
    18481 cgtatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat
    18541 gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct
    18601 gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag
    18661 acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca
    18721 aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg
    18781 gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc
    18841 aaatcgtggt cgacattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga
    18901 tgattttgat tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt
    18961 tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc
    19021 tgtatgatct ctgcgagctt gaggcgcttt cgttaaagca ccctggtctg caagtggtgc
    19081 cggtggttga acaaccggaa gcaggctggc gtgggcgtac tggtaccgtg ttaacggcgg
    19141 tattgcagga tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga
    19201 tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt
    19261 ttggcgatgc gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag
    19321 aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaagtggtt
    19381 aaacccgctc aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgccagac
    19441 caaactgaac gtctttgcgt tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg
    19501 aacaacccag cggatgaccc agcgcgatcg caccaccatt gaggttgatc ttctcgtcaa
    19561 tctgctccat tagcccgaga tctttaatgc acggcaggat ctgcgcggca aaggcttcgt
    19621 tcatctcaaa cacgccgata tcgctggcag aaagccccgc ttttttcagc gccagcttcg
    19681 aggccggaac cgggccgtaa cccataatcg atgggtcaca accaacgacc gccatcgaac
    19741 gcacacgagc gcgcggctta agacctaatt catgggcgcg gctttcactc atcaccagca
    19801 tggcagctgc gccatcggaa agtgcagaag atgtgcccgc cgttaccgta ccgtttactg
    19861 gatcaaacgc cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt
    19921 cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg accaccagtt gggatgattt
    19981 catttttaaa tgcggccgac tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa
    20041 aggcatcctg catttcacgg ctgataccgt gtatacgcgc cagcatttct gccgttaagc
    20101 ccatcatgcc cgccgctttg gctacattgc ggctcaggcc ggggtgaaaa tcgacgccgt
    20161 gactcatcgg cacatggccc atatgctcca cgccgccaac cagacatgcc tgcgcatcgc
    20221 cagtcatgat cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat
    20281 tgacggtaac cgccgggaca gagtgcggca cttctgccag cagcgccgcg ttacgggcga
    20341 tgttaaaacc ctgctccagc gtctgttgca cacaacccca gtaaatatcg tcgagggccg
    20401 ccgcttccag cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt
    20461 ccgcacgcac gttacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat
    20521 cgacaatgac aacctgttcc attgtgattc cttaagccgt tttcaggtcg ccaaccggac
    20581 gggctggctc aaccggagga tagtacggtt cgttatgacg cgctttatta cgcagacctt
    20641 ccggcacttc atacagcggg ccgaggtgct gatattgctg tgccatatcg aggtattttg
    20701 cgctaccgag ggtgtccagc cagcggaacg cgccgccgtg gaacggaggg aagcccaggc
    20761 cgtagaccag cgccatatcc gcttccgccg gagtggcgat aatgccctct tccagacagc
    20821 gcaccacttc gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat
    20881 cgcgcttcgg ttggctcact tctgccagca ggtcttcaac ggcggcgtct tcttctttct
    20941 tcggcttacc tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa
    21001 agcggttggc atcaaacagc gcgtcgatgg catcgcggta atctttctgc atccgctgcg
    21061 ggaagcctgc tgccatgaca gcctgagcgt gatgcgcggt atcaatgccc acaacgtcca
    21121 gcagatatgc cgggcccatc ggccagccaa actgtttttc catcactttg tcgatcttgc
    21181 ggaaatccgc gccgtcgcgc agcagctggc tgaaaccggc gaaatacggg aacagcacgc
    21241 ggttaacaaa gaagccgggg cagtcgttaa ccacaatcgg cgtcttgccc atcttgctcg
    21301 cccaggcgac aactttcgcg atggtttcgt cggagctttt ctcgccgcga ataatttcta
    21361 ccaacggcat tcggtggacc gggttaaaga agtgcatccc gcagaagttt tccgggcgtt
    21421 ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat
    21481 cctggcgtac tttttgttcg gtttctgcca gtacggcttt tttcactttc gggttttcaa
    21541 caaccgcttc taccacaata tccacgcggt caaatccggc gtagtcgagc gttgggtgga
    21601 tcgtggagat cacgccagcc agtttcagac catcgatctt gccgcgctca agctgcttgt
    21661 tcagcagttt cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca
    21721 tgacaaccgg cacgcctttc cacgcggact ggtaagcgat accgccaccc atgatgcctg
    21781 cgcccagcac agcagcctgt ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc
    21841 cttttacata ttgatcgtta aggaaaatgc cgaccagtgc gcgagcttcg ttggtatgcg
    21901 ccagcgggac aaaacttttg ttttccagat ttaaggcttc ttcacgacca aaacgggccg
    21961 cagcttcaat ggtttttact gcggtgatgg gggccggata atgtttcccc gccgtttgcg
    22021 cgaccattcc tttagcgatg gtgaagctca tggtggcttc aatcttgctc agttttagcg
    22081 gttccagctt cggctgacgt ttcgctttcc agtcgaggtc gccgttaatg gcctggcgta
    22141 aaatcgccat cgccccttca atcagttttt ctgctttgac tacgccatcc accagaccga
    22201 ttttcagcgc ctgatccgcg ccgacatctt taccggcggc agtgatttcc agtgcactgt
    22261 cagcgcccag catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt
    22321 tggtttccgg cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca
    22381 cgcattcgca gccaccaccc agcgcatagc cgttgacggc agcaatggtc ggcaccggca
    22441 gatcttccag gcgattaaac acgctattgg caaagtgcag ccactgactt aactgttctt
    22501 caggaacgag gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt
    22561 tgttcgaacg cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct
    22621 cgccgaggct ggcgacggtc gcagtgtcga gtttattaac tgaacctggg gcatcaaata
    22681 ccagttcggc aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca
    22741 tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca gatgcatcgg agtgtgtgtt
    22801 ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc gatctgatca cgctcggcag
    22861 aaatcacgct ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga
    22921 agggtaaaaa atggaatcac tggcctcgct ctataaaaat catatagcta ccttacaaga
    22981 acggactcgc gatgcgctgg cgcgcttcaa gctggatgca ttacttattc actccggcga
    23041 gctgttcaac gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa
    23101 agcgtgggtg ccggtaactc aggtgccaaa ctgctggttg ctggtggatg gcgtgaacaa
    23161 gccgaaactg tggttctatc tgccggttga ttactggcac aacgtcgaac cgctgccgac
    23221 ctccttctgg actgaagatg tggaagtgat cacgctgccg aaagccgatg gcattggtag
    23281 cctgctgcct gctgcgcgcg gcaatatcgg ttatatcggt ccggtgccgg aacgtgcgct
    23341 gcaactgggt attgaggcca gcaacatcaa cccgaaaggg gtgatcgact acctgcatta
    23401 ttaccgctcc ttcaaaaccg agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc
    23461 ggtcaatggt catcgtgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat
    23521 caatattgcc tatctgaccg cgaccggtca tcgtgatacc gacgtacctt acagcaacat
    23581 tgtggcgctt aacgaacacg ctgcggtgtt gcattacacc aaactggacc accaggcacc
    23641 ggaagagatg cgcagcttcc tgctggatgc cggggccgaa tataacggct atgcggctga
    23701 cctgacccgt acctggtcgg caaaaagcga caacgactac gcacagctgg tgaaagacgt
    23761 aaatgatgaa caactggggc tgatcgccac catgaaagca ggcgtcagct atgtggatta
    23821 ccacatccag ttccatcagc gcatcgccaa actgctgcgt aaacatcaaa tcatcaccga
    23881 tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat
    23941 tggccatccg ctgggcctgc aggtgcatga cgtcgccggt tttatgcagg atgatagcgg
    24001 tacacacctc gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc
    24061 gggcatggtg ttaaccatcg aaccgggtat ctacttcatt gaatcgcttc tggcaccgtg
    24121 gcgtgaaggg cagttcagca agcacttcaa ctggcagaaa attgaagcac tgaaaccgtt
    24181 cggcggcatt cgtatcgaag acaacgtggt gatccacgaa aacaacgtgg aaaacatgac
    24241 ccgggatctg aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc
    24301 gttgaagaga tcaaaaagag ccgcttcata acgatgttgg cgcataccga tggcgttgag
    24361 gcggcgaaag cgtttgttga atcggtgcgg gcagaacacc ccgatgcccg ccaccattgc
    24421 gtggcgtggg tggcgggtgc gccggatgat tctcaacagc tgggtttctc tgacgacggg
    24481 gagccggcgg gaacggcagg taaaccgatg ctcgcccagc taatgggcag cggcgtcggg
    24541 gaaattaccg ctgtggtagt gcgctactac ggcggcatat tgcttggcac cggtgggtta
    24601 gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag
    24661 acgccattaa ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg
    24721 ttgctggggc agtgtgacgg caaaattatc aacagtgatt atcaggcatt cgttctgctg
    24781 cgggtggcgc ttccggcggc gaaagtggct gaattttccg caaagctggc ggattttagc
    24841 cgtggttcat tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta
    24901 aggaagcggc agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct
    24961 tattttcagg gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg
    25021 gccgcgcttt tacccagacc ttttttgtcg ccctcgccat tggctctatg ctgtggtggc
    25081 cgaaccgcaa agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt
    25141 tttggaccgt gctgggtagt gtcggtgcgc tcccttttat cttctcggaa agcccgaacc
    25201 tcacgattac cgatgcgttt tttgaatctt tctctggcct gaccaccacc ggggccacta
    25261 cgctggtggg gctggattcg ctccctcatg ccatcctctt ttatcgccag atgctgcaat
    25321 ggtttggcgg gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg
    25381 gtgggatgca gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc
    25441 cgcgaattgc ggaaacggcg aaaactctgt ggttgattta tgtcttgctg accgtcgcct
    25501 gtgcgctggc gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg
    25561 cgactatcgc tattggcggc ttctcgacac atgatgccag tatcggttat ttcgacagtc
    25621 cggctattaa cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc
    25681 acttttcact gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca
    25741 tgtttatcgg cgtacagttt acgctggtgg ttatttgtac actcgtactg tggtttcata
    25801 atgtctacag ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtatcgatgg
    25861 cgacaaccgc cgggtttacg actgacagca ttgcccgctg gccgctcttt ttgccggtac
    25921 tgcttttatg ttcagctttt atcggcggtt gcgccgggtc aacgggcggt ggcctgaaag
    25981 tgatccgcat cctgctgctg tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc
    26041 cgaacgccgt ctatagcatt aagctgggga atcgcgcact gccggaacgt atcctcgaag
    26101 ccgtttgggg atttttctcc gcctatgcat tggtgtttat tgtcagtatg ctggcgatta
    26161 tcgccacggg cgtggatgat ttttctgcct ttgcctcagt tgttgcgaca ttgaataacc
    26221 tggggccagg gcttggcgtg gttgctgata actttaccag tatgaacccg gtggctaaat
    26281 ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgctct
    26341 ttaccccgac tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc
    26401 aacaagggac ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact
    26461 ggggatccag acggatgtcg ccaatgtgca ccgcattgaa gaaccacaat gggaaaacta
    26521 tgaccgtgtg gtcattggtg cttctattcg ctatggtcac taccactctg cgttccagga
    26581 atttgtcaaa aaacatgcga cgcgactgaa ttcgatgccg agcgcctttt attccgtaaa
    26641 cctggtggcg cgcaaaccgg agaagcgtac tccacagacc aacagctacg cgcggaagtt
    26701 tctgatgaac tcgcaatggc gtcccgatcg ctgcgcggtc attgccgggg cgctgcgtta
    26761 cccacgttat cgctggtacg accgttttat gctcaagctg attatgaaga tgtcaggcgg
    26821 tgaaacggat acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc
    26881 ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa taagcataaa gaataaaaaa
    26941 tgcgcggtca gaaaattatt ttaaatttcc tcttgtcagg ccggaataac tccctataat
    27001 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
    27061 gaattccgac agagaaagca aaaataaatg cttgactctg tagcgggaaa gcgtattatg
    27121 cacaccccgc gccgctgaga a
//
LOCUS       DAJVQH010000043        26777 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJVQH010000043 DAJVQH010000000
VERSION     DAJVQH010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.043"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(79..1230)
                     /locus_tag="OQM87_001908"
                     /pseudo
     CDS             complement(79..1230)
                     /locus_tag="OQM87_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            complement(1227..2135)
                     /locus_tag="OQM87_001909"
     CDS             complement(1227..2135)
                     /locus_tag="OQM87_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1508064.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2331..3098
                     /locus_tag="OQM87_001910"
     CDS             2331..3098
                     /locus_tag="OQM87_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1508065.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(3149..3801)
                     /gene="pphB"
                     /locus_tag="OQM87_001911"
                     /pseudo
     CDS             complement(3149..3801)
                     /gene="pphB"
                     /locus_tag="OQM87_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3907..6468)
                     /gene="mutS"
                     /locus_tag="OQM87_001912"
     CDS             complement(3907..6468)
                     /gene="mutS"
                     /locus_tag="OQM87_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCS1508066.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLLRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            6544..6723
                     /locus_tag="OQM87_001913"
     CDS             6544..6723
                     /locus_tag="OQM87_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508067.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            6755..7108
                     /locus_tag="OQM87_001914"
     CDS             6755..7108
                     /locus_tag="OQM87_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCS1508068.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(7145..9223)
                     /gene="flhA"
                     /locus_tag="OQM87_001915"
     CDS             complement(7145..9223)
                     /gene="flhA"
                     /locus_tag="OQM87_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCS1508069.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(9297..10307)
                     /gene="hypE"
                     /locus_tag="OQM87_001916"
     CDS             complement(9297..10307)
                     /gene="hypE"
                     /locus_tag="OQM87_001916"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCS1508070.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(10304..11425)
                     /gene="hypD"
                     /locus_tag="OQM87_001917"
     CDS             complement(10304..11425)
                     /gene="hypD"
                     /locus_tag="OQM87_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCS1508071.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(11425..11697)
                     /gene="hypC"
                     /locus_tag="OQM87_001918"
     CDS             complement(11425..11697)
                     /gene="hypC"
                     /locus_tag="OQM87_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCS1508072.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(11688..12560)
                     /gene="hypB"
                     /locus_tag="OQM87_001919"
     CDS             complement(11688..12560)
                     /gene="hypB"
                     /locus_tag="OQM87_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCS1508073.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(12564..12914)
                     /gene="hypA"
                     /locus_tag="OQM87_001920"
     CDS             complement(12564..12914)
                     /gene="hypA"
                     /locus_tag="OQM87_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1508074.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            13126..13587
                     /gene="hycA"
                     /locus_tag="OQM87_001921"
     CDS             13126..13587
                     /gene="hycA"
                     /locus_tag="OQM87_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCS1508075.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            13712..14323
                     /gene="hycB"
                     /locus_tag="OQM87_001922"
     CDS             13712..14323
                     /gene="hycB"
                     /locus_tag="OQM87_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCS1508076.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPACVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            14320..16172
                     /gene="hycC"
                     /locus_tag="OQM87_001923"
                     /pseudo
     CDS             14320..16172
                     /gene="hycC"
                     /locus_tag="OQM87_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            16175..17071
                     /gene="hycD"
                     /locus_tag="OQM87_001924"
     CDS             16175..17071
                     /gene="hycD"
                     /locus_tag="OQM87_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCS1508077.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            17089..18774
                     /gene="hycE"
                     /locus_tag="OQM87_001925"
     CDS             17089..18774
                     /gene="hycE"
                     /locus_tag="OQM87_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCS1508078.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTAFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            18784..19326
                     /gene="hycF"
                     /locus_tag="OQM87_001926"
     CDS             18784..19326
                     /gene="hycF"
                     /locus_tag="OQM87_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCS1508079.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KENFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            19326..20093
                     /gene="hycG"
                     /locus_tag="OQM87_001927"
     CDS             19326..20093
                     /gene="hycG"
                     /locus_tag="OQM87_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCS1508080.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            20090..20500
                     /gene="hycH"
                     /locus_tag="OQM87_001928"
     CDS             20090..20500
                     /gene="hycH"
                     /locus_tag="OQM87_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCS1508081.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            20493..20963
                     /gene="hycI"
                     /locus_tag="OQM87_001929"
     CDS             20493..20963
                     /gene="hycI"
                     /locus_tag="OQM87_001929"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCS1508082.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            21033..21965
                     /locus_tag="OQM87_001930"
     CDS             21033..21965
                     /locus_tag="OQM87_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508083.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEGGTRTDFLQSGYEDFGGNYIAHKCLHIVNL
                     DLMSYSFRTHKDRTRRCGCLLTIRKLKLKNAHHF"
     gene            21949..22260
                     /locus_tag="OQM87_001931"
     CDS             21949..22260
                     /locus_tag="OQM87_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCS1508084.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            22257..22676
                     /locus_tag="OQM87_001932"
     CDS             22257..22676
                     /locus_tag="OQM87_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1508085.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(22791..24215)
                     /gene="ascB"
                     /locus_tag="OQM87_001933"
     CDS             complement(22791..24215)
                     /gene="ascB"
                     /locus_tag="OQM87_001933"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCS1508086.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAIGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24224..25672)
                     /gene="ascF"
                     /locus_tag="OQM87_001934"
     CDS             complement(24224..25672)
                     /gene="ascF"
                     /locus_tag="OQM87_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCS1508087.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQPTVAKEVSLN"
     gene            25932..>26777
                     /gene="ascG"
                     /locus_tag="OQM87_001935"
     CDS             25932..>26777
                     /gene="ascG"
                     /locus_tag="OQM87_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCS1508088.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAP"
BASE COUNT         6124 a         7080 c         7232 g         6341 t
ORIGIN      
        1 aaaggcttca tcaccgaagt tgccagattt aagggcgagt gagacaggct tatccagtgc
       61 gtttacccac ggcacgccgg gggaaatggt tgggccaata tgaaaccctt ttatgcccag
      121 gctctgtgtg actacgccgg aggtctcacc acctgcgaca ataaagcgtg tcatgccttc
      181 cgctgctaac cgcgccgcta gttgagaaaa cagagtttct actgcctgac tggctttttg
      241 tgcaccgtat tgctgttgaa ttgctgccaa tgcgtcagtg ctggcggtgg caaaaaccag
      301 tggagcaagt acactttcct ggcccagaac ccactctgcc agttcgtgag cataagcggc
      361 cagagtttcg gttgagaggc agcgtgccac atcaacttca cgggctggtg caatttgacg
      421 gtaatgtgcc acctagcggt tggtcatttg agagcatgaa ccggagagca ctacgccgcg
      481 cccagcgagc ggacgccctg cttcgcgagc ctggttaccg ttttcttgcg cccactgccg
      541 ggccaggcca atcgccagac cagaaccgcc cgttaccagt ggggcatcgc gcaaggcttc
      601 tccctgaatt tccagatggt gttcggtcag cgcatcaagc accgcgtagc ggtagccctc
      661 ttgctgtaag cgagccagct cttgacgaac ggcatccaca ccttgttcga aaacatgtgc
      721 cgaaacgacg ccgcagcgcc ctgtggattg cgcttcaacc agacggggaa gatagctgtt
      781 ggtcatggga tttaccgggt gatggcgcat cccggattcg gccagcagtt gattcattac
      841 gaacaaatac ccctgataaa ccgtacgtcc gttgaccggc agggccggag agaagaccgt
      901 aaacggcgtg tcgagagcat ccattaatgc atcggtaacc gggccaatat tacctttcgc
      961 cgtactgtcg aaagtagagc tgtatttgaa atagatctgt ttgcaacctt gctgttgcaa
     1021 ccagctcaga gccgccagcg attgctgtgt ggcttcaacc accggacagg agcgcgtttt
     1081 caggctgatc accagtgcgt cgattgcttc cggcatttta cctgttggaa caccgttaat
     1141 ttgtaccgtt ggtagaccgt tttccaccag aaaactggcg atatccgtcg cgccggtaaa
     1201 atcatcggcg ataacgccaa tcttgatcat gatttcgctc ccggtagagt gatgccagag
     1261 aaaatcttga taactgcgct atcgtcttct ttcccgtaac ccgcgttact ggcgctggtg
     1321 aacatattca atgctgttga ggccaatggc agcgggaagt gcagggcttt ggctgtatcg
     1381 gcaaccagac caagatcctt aacaaaaata tcgacggctg aatgcggggt gtaatcgcca
     1441 tccaccacat gacgcatccg gttttcgaac atccaggaat ttccggcggc attggtcacg
     1501 acgtcataca tcacatccag cgggatcccc gcacgggctg caagtgccat cgcttcggct
     1561 ccggcagcaa tatgtacgcc cgctaacaac tggtgaataa tttttacggt cgaacctagt
     1621 cccggttctg cacctatgcg ataaactttt ccggcaacgg cttccagcac gggtgccagt
     1681 cgttcaaagg caatatcgct accggaggcc atgacagtca tttcaccgtt agcggctttt
     1741 actgcaccac cagaaactgg cgcatccagc atttccagat cgaatccagc cagagcggta
     1801 gcaatttctt gcgcatcagc actagcgata gtggaagaaa ccattactgc cgtaccgggt
     1861 ttcagatgtt gtgcaacgcc tgtttcacca aacagcacct gtttaacctg ggccgcattg
     1921 accaccagca ccagcagagc gtccagtttt tcggcaaacg tcgcggcgtt atcagaaacc
     1981 ccgcaagcac ctgcctcttt caacgtagcg caggcattgc tgttcaggtc tgcgccccag
     2041 gtagaaaaac ctgcgcggac acatgacagt gctgctccca ttcccattga ccctaagcca
     2101 acgataccga catgaaactc agatcccgtt ttcatctgct ctccttgtta atttaagtga
     2161 tattttgttt gacattgtga atataagcgc tgggagataa cgatatggtg agctgattca
     2221 cataaattaa cattttgtgt tattttatgt gaactaagcg ttagttgcgc cgcgcacgtt
     2281 tcgcaggcaa atagcgtaga atgtcagcag gacaaaggaa ggagcaaaag ttgatacccg
     2341 tagagcgtcg ccaaatcatc cttgagatgg tagctgaaaa aggcattgtc agtattgctg
     2401 aactaacgga cagaattaat gtgtcacata tgaccattcg tcgggattta caaaaactgg
     2461 agcagcaggg agccgttgtg ctggtgtccg gaggcgtcca gtctccggga cgcgtggcgc
     2521 atgaaccttc tcatcaggta aaaactgcgc tggcaatgac gcaaaaagcg gctattggca
     2581 agctggcggc aagtcttgtt cagccgggaa gttgtatcta tctggatgcg ggaacgacca
     2641 cgttagcgat agcacagcat ctgattcaca tggagtcact gactgtggtc acaaacgatt
     2701 tcgttattgc gaactacttg ctcgacaaca gtaattgcac aattattcac actggcggtg
     2761 cagtgtgtcg ggaaaaccgt tcctgtgtcg gggaagccgc tgcgaccatg ctgcgcagcc
     2821 tgatgattga tcaggctttt atttctgcat cgtcatggag tgtgcggggg atttctacgc
     2881 cagcggaaga taaagtcacg gtgaaacggg cgattgccag tgctagccgc cagcgagttt
     2941 tggtctgtga tgcgacgaaa tatggtcagg tggcgacatg gctggcgtta ccattaagcg
     3001 agtttgatca gattattaca gacgacggtt tgccggagag tgccagtcgc gcgctggcga
     3061 agcaggatct ctctttgctg gtagcgaaaa atgaataatg gcctgcaata acatctggtt
     3121 actcatgctt cacagaagaa gcatgagact accttatttt ataaaatgac agccgcccac
     3181 ttttcggcga tccggtatca atataaatct ggttagcgaa cgtctgaatg ttatcaaaca
     3241 tcatatgtcc aaatataaaa taatcagcgc cgtttatttg ttgtaactcg ccattaagcg
     3301 agttctgcac acgataaaca ggccagagta attcgctctc cgctatttct ttgtcaaaga
     3361 gatattcatc acccggataa tctgcatgtg cgatgacata ttttattatg tcgttagtga
     3421 tttcaataat atgtggaagg tgatggaatt tcagcaacag atctattgcc tcttgttgca
     3481 atcatttaaa tcgaaaaacc agtcaccacc gctggcaagc cacatattgc catcgccggt
     3541 cgcgaacgca tccagcgcca ttgcttcgtg gttgccttta acggagataa accagggctg
     3601 gtttagcagg cgcaggacgt caagactctc cggcccacga tcaatattat cgccgacaga
     3661 aataagtaag tcggtttcgg ggaaaaaaga gagttgatgt aagcgggatt gtaataactg
     3721 atattcacca tgaatatcac caacgaccca tatatggcga tagtgatggg cattgatttt
     3781 ttgatagcgt gtagatggca tggttttacc ctgtaaaata agctttccta ttatacaggg
     3841 tatttttatt tgattcgtca gttgtcgtta atattcccga tagcaaaaga ctatcgggaa
     3901 ttgttattac accagactct tcaagcgata aatccactcc agcgcctgac gcggagtcag
     3961 tgaatccggg tcgagatttt ccagtgcttc gaccgcaggc gaagtttctt ctggcactga
     4021 cagcaaagac atctgcgtac catccacttg cgtcgcggcg gcgttcggcg aaatgctttc
     4081 cagctcacgc agtttttgcc gtgcgcgctt aataacctct ttcggtacgc ccgccagagc
     4141 agcaaccgcc agaccgtagc ttttgctcgc cgcgccgtcc tgcacgctgt gcataaaggc
     4201 gatagtgtcg ccgtgctcca gcgcatcgag atgtacgttg gcgacgcctt ccattttctc
     4261 cggtagctgg gtcagctcga aatagtgggt ggcaaacagc gtcaatgcct taatcttatt
     4321 cgccagattt tctgcgcacg cccacgccag cgacagacca tcgtaggtgg acgttccacg
     4381 cccgatctca tccattaaca ccagactgta ttcggtggcg ttatgtaaaa tattggcggt
     4441 ttcagtcatc tccaccataa aggttgagcg cccggaagcc aggtcatctg ccgcgcctac
     4501 gcgggtaaag atgcgatcga taggtccaat ctcgactttt tgtgccggta catagctgcc
     4561 gatgtaggcc atcagcgcaa tcagtgcggt ctggcgcata taggtacttt taccgcccat
     4621 gttcgggccg gtaataatca acatacggcg ctgcggcgac agattcagcg ggttagcgat
     4681 aaatggctca ttcagtactt gttcaactac cggatggcga ccttcggtaa tgcgaatgcc
     4741 cggtttatca atgaaggtcg ggcaggtgta gttcagggta taggcccgtt ccgccaggtt
     4801 aaccagcacg tcgagttccg ccagcgcgct cgcgctctgt tgcaacgctt ccagatgcgg
     4861 caacagcagg tcgaacagct cttcataaag ctgtttttcc agtgccagtg ctttgccttt
     4921 tgaggtgaga actttatctt cgtactcttt tagctctgga atgatgtagc gctcggcgtt
     4981 tttcagcgtc tggcgacgca tgtagttgat gggtgccaga tggctttgcc cacggctgat
     5041 ttgaatgtag tagccgtgca ccgcattaaa gccaactttt agcgtgtcca ggccggtacg
     5101 ttcacgctcg cggacttcca gacgctccag ataatcggtc gcgccgtcag ccagcgcgcg
     5161 ccactcatcc agctcttcgt tatagcccga tgcgataaca ccaccgtcgc gtaacagcac
     5221 cggcggtgtg tcgatgattg ctcgctccag cagatcgcgc agctcggcaa actctcccat
     5281 cttctcacgt agcgcctgta ccggtgcact atcgacagtt tctaactgcg cacgcagctc
     5341 cggcagttgc tggaaagcgt ggcgcatacg ggccagatcg cgtgggcgag cagttcgtaa
     5401 agccagacgt gccagaatac gttccaggtc gccgacctga cgcagtaccg gctgcaactc
     5461 ggcggtgaaa tcctgcaatg cgccaatagt ttgctggcgc tcaagcaaca cgcgagtatc
     5521 gcgcactggc atatgcagcc agcgtttcag catacgacta cccatcggcg tgacggtgca
     5581 gtcgagcaca gaagccagcg tattttccgc accgcccgcc aggttctgag taatttccag
     5641 gttacgacgt gtcgcggcat ccataatgat gctgtcctgc tcacgttcca tggtgataga
     5701 acgaatatgc ggcagggtcg tgcgttgggt atctttcgca tactgcaaca gacaaccggc
     5761 agcacaaagt ccgcgcggcg cgttctcgac gccaaaaccg accagatcgc gggtgccaaa
     5821 ttgcagattc aactgctggc gcgcggtgtc gatttcaaac tcccacagcg ggcgacggcg
     5881 caggccgcga cggccttcaa tcagcgacat ctcggcgaaa tcttctgcat acagcagttc
     5941 cgccggatta gtgcgttgca gctctgccgc catcgtttcg cggtcggccg gttcgctcag
     6001 gcgaaaacgc ccggagctga tatccagcgt cgcgtagccg aaacctttgc tgtcctgcca
     6061 gatagccgcc agcaggttgt cctgacgctc ctgtaacagg gcttcatcgc tgatggtgcc
     6121 tggcgtaacg atacgcacaa ctttgcgctc aaccggacct ttgctggtcg ccggatcgcc
     6181 aatttgttcg cagatggcaa cggactctcc ctgattcacc agtttggcga gatagttttc
     6241 caccgcatgg tagggaatcc ccgccatcgg gatcggctct cccgccgaag caccgcgttt
     6301 ggtcagtgaa atatccagca gttgcgacgc gcgttttgcg tcgtcataaa acagttcata
     6361 aaaatcaccc atccggtaaa acagcaggat ctcgggatgc tgggctttca gcttgagata
     6421 ctgctgcatc atgggcgtat gggcgtcgaa attttctatt gcactcatgg ggttatgtcc
     6481 ggttccctga tattaaatgg ggtgtgatgg tttttatttt cgttgtaatc acataaggcg
     6541 cacatgatac cggagttaat catttcaggg gaagaagcga acggcgagtt cttgatgctg
     6601 gttccgggaa agatgctgtt ttgcagcttt tgcgtgatgc ttatgaagca agtacgcaaa
     6661 attgtatcga ttggcgaacc cttcttcttt agagtcatag tgccctttaa gattaagatg
     6721 taatataaat gcatcttaat gaggtggagg cagcatgtcc ggcaagcgta tctctcgtga
     6781 aaaactgacg attaaaaaaa tgatcgatct ttatcaagcg aaatgcccgc aggcgtcagc
     6841 ggagccggag cattacgagg cgttgtttgt ttacgcgcaa aagcggctgg ataaatgtgt
     6901 gttcggcgaa gagaaaccag cctgtaagca gtgtccggtg cactgttacc agcctgcaaa
     6961 gcgtgaagag atgaaacaga ttatgcgttg ggccgggccg agaatgctgt ggcgtcatcc
     7021 gatcttaacc gtgcgtcatc tcatcgatga caagcgtccg gtgccagaac tgccggaaaa
     7081 atatcgtccg aaaaaacctc gtgaatagta tgccggacag gcagatctgt ccggcaattt
     7141 gcagttaaat caatgccgat ttatcaattc ccagccgctt catccgtgac agcagggtcg
     7201 tgcgtttcag ccccagacgt tgcgcagcgc ctttaggccc ggcaaccacg ctgttggttt
     7261 ctttcagcac gcgcacaatc aactgatatt catcttcgcc ctcctgggcg acaaccgttg
     7321 cggcaggcgg cgtttcaggc tccggtaaag caatatctgg caatgacagc tgcagcacgt
     7381 tgccgcgtgt tagcaatact gcgcgctcaa tgacgttttc cagttcgcgt acgttacccg
     7441 gccactccat gttgctcaag gtgcgcagcg tctcggcagg aatgctgtcg atattgcgcc
     7501 ccagacgacg ggcaattttg aaggtaaagg ctttcgccag cagcggaata tcttccggac
     7561 gctcgcgtag tggcggcagg tgaatcggga atacgttcag gcggtaatag agatcgctac
     7621 ggaactctcg gtcggcgacc atttttttca gatcgcggtt agtcgcggca attaaacgta
     7681 cgtccgtctg aatgattttg ttgctgccga gacgttcaaa ctcctgttcc tgcaatacac
     7741 gcagcaactt cggctgtaac tccagtggca tatcgcccac ttcgtcgagg aacagagagc
     7801 ttttatccgc cagttcaaaa cgaccaatgc gctgggcgct ggcaccggta aaagccccac
     7861 gctcatgacc aaacagatcg ctttccagca atccggcagg catcgccgcg cagttcattt
     7921 tgaccatgcg gcgattatta cgcccactga gattatggat cgcacgggca atcagctctt
     7981 tacccgtgcc agtttcaccg aggatcagca cggtactgtc actttgcgcc accatttcaa
     8041 cttgtttaag cacgctgtac atggcttcgc tgcggccaat aatctcgcca aattcactat
     8101 caacattgtt gagttgctcg gtcagggcga ggttttcatc aaccagccgt tctttcagac
     8161 gatggatttc ctgataggcg agggcgttat cgacagcgat tgccacacgt tcggcaatct
     8221 ggcgcagcaa attcagattg gtagtggtaa acactttctc ttcgcattgc gccagtttca
     8281 gcacgcccag catggtgtcg ccagacatca gcggtaacag gcacaaggtt tgaatctggt
     8341 tgccccaggt gtcgaacaac atgcgttcat agggggctaa atcgtcccgc tcgtagagat
     8401 tgatcagcag catctcttta cttttgaaca cgcgttcggt gagggttccg gcttcatcga
     8461 cttcgctctg ttcgtgggcg ggatgttgtt tatcaagata gtgagtggag tagatgttga
     8521 gtttgttttt acggtggctg cgtaagacaa tgctgatatc gtcaatgtcg aaatagtaat
     8581 ggatttcttt ggcgacttcg ctgaccagtt cgtccatatc caggcgggaa agcaccgcgt
     8641 tggtaatggc aaccaggatg cggaagttat cgcgctcccg gcataacaac tcatagtcga
     8701 cattgttgac gacgcggctc tggatttgtt cggtgacgac agaaacgatc tgcgtaaatg
     8761 tttgcagacg attgaactct ttttcgctcc agggacgatc gtcataacga ataaattcac
     8821 agccaccaaa aatatgccct cccgccgcca gtggcatcag gcaatagtga ccaaatttgg
     8881 ggtatagccc acctgcggcc agctgcggcc aggtttcaca aaattcttcg taactgcaat
     8941 gcagcgtatc agggcgcgac aaaatgctgc gtaccggacc gtgtgccaga acagtttcgt
     9001 cttcatattt aatgggggtg tctttttcac gcgacgcgta ataagacgca cgttgagtct
     9061 gcgcttgcca caacacaata gccgcgttgt cggcgagcgc agaacgcttt accagttgcg
     9121 aaagagcctc acacagcgag gccagatcgg gctgctgcaa tagtgtccga gtgatgtcga
     9181 acaacccttg ctgtccgaga tcactcatcg gtgtatatga cattttgttc ttccaggaaa
     9241 gcaccgcaaa gcggtgcaaa aggtcattga ctaactatag gagatttaga attttattag
     9301 catatacgcg gaagcggttc ggcgtgtggt aaatcgaggg ttcgtttcac gccatacaga
     9361 ccggcaagac gaacaccttt acgttccacc acttcaccaa tcagcgccgc gtctttcccc
     9421 agtggatggg aatgtaacgc tgccagcact tgctctgccg cgttgcgttc aacggcgatc
     9481 accagtttac cttcgttggc aaagttcagg gcgtccagtc ccagcaattc gcaaacgccg
     9541 cgcacggcag gtttaaccgg cagcgctgat tcagaaattt caataccaca accgcaggct
     9601 gccgcgaact catgaaccac cgcgtttaca ccaccacggg tggcatcacg cagcgctttc
     9661 acgccgggaa tgtcacgcag cgtctgaata agcggcgtca gcaccgcgca gtcgctgacc
     9721 agttcgccat ccagccccag ctgctcacgc aggttaagga tagtcgcccc gtggtcgccg
     9781 agtgtaccgc taaccagcaa tacatcgcct gcggttagcg tctgtgcgcc ccagtgaata
     9841 ttcgccggaa ttgcgcccat gccagcggtg ttgataaaca gtttatctgc cgcgccgcgc
     9901 tgcaccactt tagtatcgcc agtaacgatg gcaatgcctg ccgtgcgggc ggtttctgcc
     9961 atgctggtca ctgcggcttt cagtgtctcc atcggcaatc cttcttcgag gataaagcca
    10021 caggagagat agcgcggaat agcgccactg accgcaacgt cattcgcggt gccgcaaatc
    10081 gccagcttgc cgatattacc gccaggaaag aacagcgggt caatgacgta actgtcggtg
    10141 gagaacgcca gacggtcgcc ttccgctacc agctgcgcca gatcaagacg tgcctgatct
    10201 tcctgctctg ccagccacgg gttggcaaag gcttccataa acaggctgtt gattaattgc
    10261 tgcatcgcct ggccgccgct gccgtgggcg agttggatat tattcacgct tcactctcct
    10321 gctgacgata ctgataccac gcggcgcacg ctccttcgga ggagaccatc agtgcaccaa
    10381 atgcggtttg aggattacag gtgttaccaa acagcgggca ttgatgcggc ttacatttgc
    10441 ccgttaatac ctcaccacaa cgcgcgcgcg ggtcatcgca gacctgctgc ggtgccgggc
    10501 ggaaatgtgc ttcggcatcg aatcgttgat aatccggcgt caggtgcacg ccagaagatt
    10561 caatcacgcc taagccgcgc cattcgctgt cgccgttgac acagaacaca tcggcaatcg
    10621 cctgttgcgc cagcaggtta ccggcatccg gtaccacccg acgatactga ttctctacct
    10681 tgctgtgggc cgctattttc tgctccacca gcatgaccac gccttgcagt agatcaaggg
    10741 gttcgaaacc agcgaccacc agcggacgat gaaaatcgct ggcgataaaa ttataggcat
    10801 cagtgccgat aaccatacta acgtggcctg gcgcgaggaa cgcgtcgata ccgttatccg
    10861 gctgttccag caaactgcgc agcgtcggga taagcgtaat gtgctggcag aagaagtaaa
    10921 aattctgcac atcacgcgct ttcgcctgtt gcagagtgat ggcggtggtc ggcatggtgg
    10981 tttcaaaacc taagccgaag aacaccactt tgcgggttgg attctcctgc gccagtttca
    11041 acgcatccat cggcgagtaa acgatgcgca catcggcacc gcgtgctttt gcctgcaaca
    11101 gcgatccctg tttccctggt acgcgcatgg cgtcgccaaa ggtacagaag atgacttccg
    11161 gatggctggc aatctccacg caggtgtcga ttctacccat cggcagtacg cacaccgggc
    11221 aacccggacc gtggataaac tcaacgtttt caggcagtaa ctggtcgagg ccgaatttaa
    11281 aaatggcgtg ggtatgaccg ccacacactt ccataatccg cagagggcgt tcggcggtgt
    11341 aagagagatg tgaagcacgt tcgcgcagat gctcaattaa ctgcatcacc tgttccggcg
    11401 cgcgatattc atcaacaaaa cgcattattt ttcctcgcca tacaacagcg caccgacatc
    11461 cggctcaacg tcaaacatgt tttgtaaggc gtcgagagtg tcgcgtgctt cggcttcatt
    11521 aattacgctc atggcaaagc caacgtgaac cagtacccac tggcccacgc gcggctgacc
    11581 gttttcatcg cagctgccga ctaacgttaa atcgacatcg cgctgaatgc cgcagacgtc
    11641 gactttcgcc tggttgccgt caatggtgcg gatctggccg ggaacgccta tgcacatcgc
    11701 tgtgtctcca gccagttcag ccactggtcc atcccttcgc cgctggtggc ggaaataagg
    11761 atgatttcaa tttctggatt gacttcgcgg gcgcaggcga tgcacttctc aacgtcaaag
    11821 ttgagatacg gcaacaggtc aactttgttg agcagcatca gcgaggcggc ggcaaacata
    11881 tgcggatatt tcagcggttt gtcttcacct tcggtaacgg aaagcaccgc tactttgtgt
    11941 ttttcaccga gatcgaagct ggccgggcat acgaggttgc caacgttttc gataaacaga
    12001 ataccgttat cgtccagtgg cagacgcggt gcggcgtcgg caatcatctg tgcgtcaaga
    12061 tggcagcctt taccggtgtt cacctgaatc gctggtgtac cggtagcgcg aatgcgtgcg
    12121 gcatcgttca cggtttgctg gtcgccttca ataaccgcgc acggaacgct gtctttcagg
    12181 cgcattaagg tttccgtcag cagggtggtt ttaccggaac cagggctgga aaccaggttg
    12241 agcaccagtt gcttgcgggc agcaaagcgc gcgcggttgc gttcagccag acggttgttt
    12301 ttgtccagca cgtcaatttc gacttccagc atccgacgct ggctcatgcc cggtgtgtga
    12361 gtgcccgctt cgccatgacc gtaatgcagg tcgccttctt cagtctggct gggggtaaat
    12421 tcaggcgctt taatgccggt gattttcatc tgcgggcgtg ccgccggggc aaatggcgcg
    12481 ctacgaaacg cggaatgagg gttatgttca tcaccctcga tatacaggtt gccttcaccg
    12541 caaccgcatg ttgtacacat cgctcactcc tggtctattt ctatccgccg aatctgtaaa
    12601 ccgtcgtctg ccacaatctg tagcatgtca ccatgacact gtggacagcg gcggacgcgc
    12661 tgggtcagta gcgtcacata ctgttggcat gtttcacacc agcattcggc ctcttgttct
    12721 tcgaggtgca gtttacaacc ttccgccacg ctgccgcggc aaaccagatc aaaacaaaag
    12781 gcaagagagc tggtttcgac acaagaaaat gcgccaattt tgagccagac cccagttacg
    12841 cgttttgcgc cgtgttttgc ggcctgctgt tcgatcaatt ccagtgcccg ttggcagagg
    12901 gttatttcgt gcatatcgcc tcccattaac tattgccagc tacaagcaat aattgcgcca
    12961 gtgttgatta tccctgcggt gaataatgtc gatgatgtcg aaatgacacg tcgacacggc
    13021 gacgaaattc atctttagct taaaaatctc tttaataaca ataaattaaa agttggtaca
    13081 aaaaatgctt aaagctggca tctctgttaa acgagtaacc tgacaatgac tatttgggaa
    13141 ataagcgaga aagccgatta catcgcacag cggcatcgtc gcctacagga ccagtggcac
    13201 atctactgca attcgctggt tcaggggatc acgttatcga aagcgcgcct gcatcacgcc
    13261 atgagctgcg cgccggacaa agaactctgt ttcgtccttt ttgaacattt tcgtatttac
    13321 gtcaccctgg cggatggctt taacagccac accatcgagt attacgtcga aacaaaagat
    13381 ggcgaagaca aacagcggat tgcgcaggcg caactgagca ttgacggcat gattgatggc
    13441 aaggtcaaca tccgcgatcg cgaacaggtt ctggaacact atctcgaaaa aatcgctggc
    13501 gtttacgaca gcttatacac cgccattgaa aacaatgtgc cggtgaattt aagccaactg
    13561 gtaaagggac aaagcccggc agcatgagct gaggctttgc ccgttttgca ggcgttacgc
    13621 ctgtttgggg atgggcgtgt cgatgagtgt cgaaaatgac atttcatcgg catgttttcg
    13681 tcaaaaatga caatcacctg aggaatgcct ggtgaatcgt tttgtaattg ccgactccac
    13741 gctctgtatc ggctgccaca cctgtgaggc cgcctgttca gagacgcatc gccagcacgg
    13801 cctgcaatca atgccgcgcc tgagagtgat gctcaatgaa aaagaatctg cgccgcagct
    13861 ctgtcaccac tgtgaagatg caccctgcgc ggtggtctgc ccggttaacg ccatcacccg
    13921 cgttgatggg gccgtgcagt tgaatgaaag tctgtgcgta agctgcaaac tgtgcggcat
    13981 cgcctgcccg tttggcgcaa ttgaattttc cggcagccgt ccgctggata ttccggcaaa
    14041 cgccaatacc ccgaaagcgc caccggcacc gcctgctccg gcgtgtgtca gcacattgct
    14101 tgactgggtg ccaggtattc gcgcgatcgc cgtcaaatgt gacctttgta gctttgatga
    14161 acaaggtccg gcctgtgtgc ggatgtgccc gactaaagcc ctgcatctgg tggataacac
    14221 cgatatcgcc cgcgtcagca aacgtaagcg tgagctgact tttaacacgg actttggcga
    14281 tctcaccttg tttcagcagg ctcaaagtgg agaggctaaa tgagcgcaat ttccctgatc
    14341 aatagcggcg tggcatggtt tgtcgccgcc gctgttctgg catttctctt ttcttttcaa
    14401 aaagcgctaa gtggctggat agccggaatt ggcggcgcgg ttggtagtct gtatacggca
    14461 gccgcgggct tcactgtact gactggctcg gttggcgtga gcggtgcgct gtcgctggta
    14521 agctacgatg tgcaaatctc cccgcttaac gcgatttggc tgattaccct cggtctttgc
    14581 ggtctgtttg tcagcctcta taacattgac tggcatcgcc acgcgcaggt gaagtgcaac
    14641 ggcttgcaga tcaatatgtt gatggctgcc gccgtctgcg ccgtcatcgc cagcaacctc
    14701 ggcatgttcg tggtaatggc cgaagtcatg gccttgtgtg cggtgttcct caccagcaac
    14761 agcaaagagg gcaaactgtg gtttgcgctg gggcgtcttg gcactctgct gctggtgatt
    14821 gcttgctggc tgctgtggca gcgttatggc acgctggatc tgcgcctgct ggatatgcgt
    14881 atgcaacagc tgccgctcgg ttccgatatc tggctgctcg gttccgatat ctggctgctc
    14941 ggtgtgattg gctttggcct gctggctggg attattccgc tgcacggctg ggtgccgcag
    15001 gcacatgcta acgcctctgc gccagctgcc gcgttgtttt ctacggtagt catgaaaatt
    15061 ggcctgctgg gcatgttaac cctgtcactg ctgggcggta atgcaccgct gtggtggggg
    15121 atcgcgctgc tggtgctcgg catgatcacc gcgttcgtcg gtggtctgta tgcgttgatg
    15181 gaacacaaca tccagcgcct gctggcttac cacaccctgg aaaatatcgg cattatcctg
    15241 ctggggctgg ggctggggct ggcgtaacgg gtatcgcgct cgaacaaccg gcgctgattg
    15301 ctcttggcct ggtcggtggt ctgtaccatc tgcttaacca tagcctgttc aaaagcgtac
    15361 tgttcctcgg ggcggggagc gtctggttcc gtaccggtca tcgcgatatc gaaaaactcg
    15421 gtggtattgg caagaaaatg ccggttatct ccatcgccat gttagtcggg ttgatggcaa
    15481 tggctgcgct gccgccactg aacgggtttg ccggggaatg ggttatctat caatccttct
    15541 tcaaactgag caatagcggc gcgtttgttg cccgtctgct ggggccgctg ctcgctgtgg
    15601 gtctggcaat taccggtgcg ctggcggtga tgtgtatggc gaaagtctat ggcgtcacgt
    15661 tcctcggcgc gccgcgtacc aaagaagccg aaaacgccac ctgtgcgccg ctcctgatga
    15721 gcgtaagcgt agtggcactg gcgatttgct gcgtaattgg cggtgttgct gcgccgtggc
    15781 tgctgccgat gctctctgct gctgtacctc tgcctctgga gcctgctaac accaccgttt
    15841 ctcaaccaat gatcacattg ctgctgattg cctgcccgct gctgccattc atcattatgg
    15901 cgatttgcaa aggcgaccgc ctgccgtcac gttcccgcgg tgcggcctgg gtgtgcggct
    15961 acgaccatga aaaatcaatg gtgattaccg ctcacggttt tgccatgccg gttaaacagg
    16021 cgtttgcgcc agtgctgaaa ctgcgcaaat ggctgaatcc ggtgtcgctg gtgccgggct
    16081 ggcagtgcga aggtagtgcg ttgctgttcc gccggatggc gctggttgaa ctggcggtac
    16141 tggtggtgat tattgtttca cgaggagcct gagaatgagt gttttatatc cgttaattca
    16201 ggcgctggtg ttatttgccg ttgcgccgct gctctccggt attacccgcg tggcgcgcgc
    16261 ccgtctgcat aaccgtcgcg ggccgggcgt gttgcaggag tatcgcgaca ttatcaaact
    16321 gctgggccgc cagagcgttg gcccggatgc ctctggctgg atgttccgcc tgacgccgta
    16381 tgtgatggtg ggcgtcatgc tgactatcgg ttctccgctg ccgcaactgg gcgatttgat
    16441 caccttactg tatctcttcg ccatcgcgcg tttcttcttt gccatttccg gtctggatac
    16501 cggtagcccg tttaccgcta tcggtgcgag ccgtgaagcg atgcttggcg tgctggtcga
    16561 accgatgttg ctgcttggtc tgtgggttgc cgcacaggtt gccggttcca ccaacatcag
    16621 caacattacc gacaccgttt atcactggcc gctgagccaa agcatcccgc tggtgctggc
    16681 gctctgcgcc tgtgcgttcg ccacttttat cgaaatgggc aaactgccgt tcgacctggc
    16741 ggaagccgag caggagttgc aggaaggtcc gctctccgaa tacagcggta gcggctttgg
    16801 cgtcatgaaa tggggtatca gcctgaaaca gctggtggtg ttgcagatgt tcgtcggggt
    16861 gtttattccg tggggacaaa tggaaacctt caccgtcggt ggactgctgc tggcgctggt
    16921 gattgccatc gtcaaactgg tggtcggcgt cctggttatc gcgctgttcg aaaacagtat
    16981 ggcccgtctg cgtcttgata ttactccgcg cattacctgg gctgggtttg gctttgcatt
    17041 tttagcgttc gtctccttgc tggcggcgtg attaaagaga gtttgagcat gtctgaagaa
    17101 aaattaggtc aacattatct cgccgcgctg aatgaggcat ttccaggcgt cgtgctggac
    17161 cacgcctggc agaccaaaga tcagctgact gtcaccgtaa aggtgaacta tctgccggaa
    17221 gtggtggagt ttctctacta caagcagggt ggctggctgt cggtgctgtt tggtaacgac
    17281 gaacgcaaac tgaatggtca ttacgccgtt tattacgtgc tgtcgatgga gaagggcacc
    17341 aagtgttggg taacggttcg cgtcgaagtt gacgccaaca aaccggagta tccgtccgtg
    17401 acgccgcgcg ttccggcggc ggtgtggggc gagcgcgaag tgcgcgatat gtacggtttg
    17461 attccggttg gtctgccgga tgactggccg gatgaacttt atccgctgcg taaagacagc
    17521 atggattatc gtcagcgtcc ggcaccgact accgatgctg aaacctacga gttcatcaac
    17581 gaactgggcg acaagaaaaa caacgtcgtg ccgattggtc cgctgcacgt cacttctgac
    17641 gaaccgggtc acttccgtct gttcgtcgat ggcgaaaaca ttatcgacgc cgactaccgt
    17701 ctgttctacg tccatcgcgg tatggaaaaa ctggcggaaa cccgcatggg ttataacgaa
    17761 gtgaccttcc tctctgaccg tgtgtgcggg atctgcggct ttgcccacag caccgcctac
    17821 accacgtcgg tggaaaacgc gatgggtatt caggtgccag aacgcgcgca gatgatccgc
    17881 gccattctgc tggaggtgga acgcctgcac tcgcatctgc tcaacctcgg cctcgcctgt
    17941 cactttaccg ccttcgactc cggctttatg cagttcttcc gcgtgcgtga aacctccatg
    18001 aaaatggcag agatccttac cggtgcgcgt aaaacctacg gcctgaacct gatcggcggg
    18061 attcgtcacg atctgctgaa agatgacatg atccagaccc gccagctggc gcaacaaatg
    18121 cgtcgtgaag tgcaggagct ggtggatgtg ctgctgagta cgccgaacat ggaacaacgc
    18181 accgtcggca ttggtcgtct ggacccggaa atcgcccgcg atttcagtaa cgttggcccg
    18241 atggttcgcg ccagcggtct cgcccgcgat actcgcgccg atcacccgtt tgtcggttat
    18301 ggcctgctgc caatggaagt ccacagcgag cagggctgcg acgttatttc ccgtctgaaa
    18361 gtgcgaatca acgaggtcta taccgcgctg aatatgatcg actacggtct ggataacctg
    18421 ccgggcggtc cgctgatggt ggaaggcttt acctacattc cgcaccgttt cgcgctgggc
    18481 tttgccgaag cgccgcgcgg cgacgatatc cactggagca tgaccggcga caaccagaag
    18541 ctgtaccgct ggcgctgccg tgccgcgacc tacgcgaact ggccgaccct gcgctacatg
    18601 ctgcgtggca ataccgtttc cgatgcgccg ctgattatcg gtagcctgga cccttgctac
    18661 tcctgtaccg accgcatgac cgtagtcgat gtgcgtaaga agaagagcaa agtggtgccg
    18721 tacaaagaac tcgagcgtta cagcattgag cgtaaaaact cgccgctgaa ataaggaatc
    18781 gccatgttta cctttatcaa aaaagtcatc aaaaccggca cggcgacctc gtcttatccg
    18841 ctggagccga ttgcggttga taaaaacttc cgtggtaagc cagagcagaa cccgcagcag
    18901 tgcatcggct gcgcggcttg cgtcaatgcc tgcccgtcaa acgccttaac ggttgaaact
    18961 gacctcgcca caggagagct ggcgtgggag tttaatctcg ggcgctgcat cttctgtgga
    19021 cgctgcgaag aagtttgccc gacggcggcg atcaaactgt cgcaagagta cgaactggcg
    19081 gtgtggaaga aagaaaactt cctgcaacag tcccgcttcg cgctgtgcaa ctgccgcgtc
    19141 tgcaatcgtc ctttcgccgt acagaaagag atcgactacg ccattgcgct gcttaagcac
    19201 aatggtgaca gccgcgcgga aaaccaccgc gaaagctttg agacttgccc ggaatgtaag
    19261 cgccagaaat gcctggtgcc gtccgaccgt attgaactga ctcgccatat gaaagaggcc
    19321 atctgatgag caatttatta ggcccccgtg acgccaacgg cattccggtc ccgatgacgg
    19381 tggatgaatc catcgccagc atgaaggcgt cgttactgaa aaaaatcaaa cgttctgcct
    19441 atgtttaccg cgtggactgc ggcggctgca acggctgcga aatcgaaatt ttcgccacgc
    19501 tttctccgct gttcgatgca gaacgcttcg gcattaaagt cgttccttca ccgcgtcatg
    19561 cggatatttt actgtttacc ggcgcggtca cccgtgcaat gcgatcccct gcgctgcgtg
    19621 cgtggcagtc cgcgccggac ccgaaaatct gtatctcata cggtgcctgc ggtaacagcg
    19681 gcgggatctt acacgatctc tactgcgtgt ggggcggtac ggataaaatt gtccctgtgg
    19741 atgtttatat ccctggctgc ccgccaacgc ctgccgccac gctgtacggc tttgcaatgg
    19801 cgctcggcct gctggagcag aaaattcacg cccgtgggcc gggtgaactg gatgaacaac
    19861 cggcggagat cctgcatggc gatatggtgc agccgctgcg cgtgaaagtg gatcgcgaag
    19921 cacgtcgcct ggcgggttat cgttacggtc gtcagattgc cgatgattac cttacacagt
    19981 tagggcaggg cgaagaacag gttgcacgct ggctggaagc ggaaaacgat ccgcgtctga
    20041 acgagattgt cagccatctg aatcatgttg ttgaagaggc gcgtatccga tgagtgaaaa
    20101 ggtggtgttc agtcaactga gccgtaaatt tattgatgag aacgatgcca cgcccgccga
    20161 ggcgcagcag gtggtctatt acagcctggc gattggtcac caccttgggg ttatcgattg
    20221 cctggaagcg gcgctcacct gcccatggga tgaatatctg gcatggattg ccactctgga
    20281 ggcgggcagc gaagcccgcc acaaaatgga aggcgtaccg aaatatggcg agatcgtcat
    20341 cgacattaac catgtgccga tgctggccaa cgcattcgat aaagcccggg cagcgcaaac
    20401 ttcgcagcag caggaatgga gtacaatgct gttaagtatg ctgcatgata ttcatcagga
    20461 aaacgccatc tatttgatgg tgaggagact gcgtgactga cgttttactc tgtgttggca
    20521 atagcatgat gggcgatgat ggcgctggcc cgctgctggc ggaaaagtgc gccgccgcgc
    20581 cgaaaggtaa ctgggtggtg attgacggcg gtagtgcacc ggaaaacgac atcgtcgcca
    20641 tccgtgaact gcgcccgaca cgactgctga ttgtcgacgc cacggatatg gggctaaacc
    20701 ccggcgaaat ccgcatcatc gacccggatg atatcgccga gatgtttatg atgactaccc
    20761 ataacatgcc gttgaattac cttatcgacc agttaaaaga agacattggt gaagtgattt
    20821 tcctcggcat tcagccggat atcgtcggct tttactaccc gatgacccag ccgattaaag
    20881 atgcggtaga aaccgtttat caacgactgg aaggctggga aggaaatggt ggatttgcgc
    20941 agttagcggt ggaagaagaa taattctcca tcagggaatt cagggaattc agggaattca
    21001 gggaattcag ggaattgaac atcagaacag ggatgtgcgc ttttttattg agcttagtgc
    21061 tgcctgcaca ggccaccagc tttaccgaat atctgccaat gagtgacagc gaatacgcgc
    21121 aaaagcgggc gctgaaacct ctcctgacca tgccttatga tgccgagcaa acctggcatt
    21181 ttaggaaagt gggcgttgct ggcgtgacgc ttgagaagat gccaaatgaa gaagattact
    21241 ggcaattaac cgctaaagac cgcgccggta aatcctggac tgtgcctgtg ggtatgctgg
    21301 caaacatagc gggcaaaggg caactctatc gcgccgatct tgatcgtaac ggcattcagg
    21361 atctggttat ctggttacca agtaccggta atggtctggc tccttatgca catttaattc
    21421 ttatgacgtt cacgcgcgaa ggccgtccct gtgtctttga acccagggga ttctacaccg
    21481 caagcaaaac tggcgtagat gatttgctgg atttgcaagg caatggtcac acgcaattgc
    21541 tggatatgca gtttaattct ggttactgga taaccagtct gtatcaggta aaagacgcca
    21601 aatggcagcg tgttcacggc tggtttgaca agttaagcta tcccgcgctc acgcgtttta
    21661 cttatacccc caaccgtaaa ctcgtcctca aacccatcgc cgggcgcgac ccgcaaacgg
    21721 aagatctggc gcagacgcag cgttgcttga taaaggggga tgtgcttgag gggggaacca
    21781 gaactgactt tttgcagagt ggctatgagg attttggggg taattatatc gcgcacaagt
    21841 gtttacatat tgtgaacttg gatttgatga gctatagttt ccgaacacat aaggatagaa
    21901 ctcgcagatg tggctgttta cttactataa ggaagttaaa acttaaaaat gcacatcatt
    21961 tctaaagcgc catttgaaga gtctgcgcgt aaatatccta acgacgctct ggctcttcag
    22021 gcattgtatc gggttattaa ggaaacggat ttttctacgc cagaagagat gcgaacggcg
    22081 tttcccaacc tcgacaattt taaatatcgc aacaaatggt acgttctgga tgtaggtggg
    22141 aacaacctag gggtgattgc ctacataaac tttgttaata agcggttctt tgtgaagcac
    22201 ataactaatc acgccgagta tgacaaactg acacgttact acagagagaa caaagaatga
    22261 ctgctaatgc tgcacacgct gttaaagcaa caagagaact ggtcaatgca gtcccctttc
    22321 tgggaggtag tgactcagaa gatgattatc gtgaagcact ggaactggtc gaatatttga
    22381 ttgaagaaga tgacaccaat ccacttatcg actttctggc gagccgcatt gcagaatatg
    22441 aaaacaacaa ccaagagttt gcagagttcg acaaagccgt tgcggcaatg cctgttggcg
    22501 tggcgttact tcgcacgctg attgatcaac ataatctgac ctatgcggat ttgaagaacg
    22561 aaatcggctc taaatccctg gtcagtcaga ttttgtctgg acaacgatcc ctgacgatat
    22621 cccacattaa ggcactttca gcccgatttg gcgttaagcc ggaatggttt ctttgatttt
    22681 tgactaatgc caaatgctaa attgcccgat gcgctgcgct tatcgggcct acatgtcgca
    22741 cgaactatgt aggccggata aggcgttcac gccgcatccg gcactgttac ttactctaaa
    22801 tcttccccat tactggcaat cactttttta taccaccaga acgatttctt acgtgtgcgc
    22861 gtcagcgtgc cgttgcctgc gtcgtcacgg tctacgtaga caaaaccgta gcgtttgctc
    22921 atttcacccg tagaggcgga aactaaatca atacagcccc atgtggtgta gcccatcagc
    22981 ggaatgccat ctgcaatcgc ttcgcctatt gcgcggatat gttcgcgtaa atagctgatg
    23041 cgatagtcat cgttaatctc gccattggca gcaagttcat cttttgcgcc gaggccgttt
    23101 tccaccagaa acagcggctt ctgataacga tcgtacatca tattcatggt gatacgcaga
    23161 ccgagtggat caattcccca gccccagtcg ctcacttgca gatacgggtt acgcagcgat
    23221 ttcaccacgt tcgccgcact gctgttgttg gcgttcatct ccgccgaggc gcagcgcgag
    23281 gcgtaatagc tgaaagagac aaaatcgacg gtgtttttca ggatctcatc atcgcccagc
    23341 gctttatcta tggttacccc tttttcgcga aatacgcggg cagagtaagc cggatacgcg
    23401 ccgcgcgcct gcacatcgat aaaaaacagg ttttcccgat ctttctccag cgccgcccag
    23461 acatcttccg gtttgcagct gtaagggtag aagttaccgc ccgccagcat acagcccacc
    23521 tgattttgcg ggttaacctc atgggcgatt ttggtggcta gcgcactggc aaccagctgg
    23581 tgatgcgcgg cctgatattt cacctgatcc tgattttcac cttcttcaaa caccagaccc
    23641 gcgccggaga acgggctatg caacataatg ttgatttcat tgaaggttag ccagtatttc
    23701 accagaccat caaatgcttc aaagcaggtt ctggcgtagc ggctgaaaaa ctccaccagc
    23761 ttgcggttac gccaggagcc atattcggtg accagatgca tcggcacatc gaagtggcac
    23821 aacgtgacca gcggttcgat accgtacttt ttacactctt caaagacagg acgataaaaa
    23881 gcaatgccct gctgattggg cgtgatttca tcgtcctgcg gaaagagacg gctccaggca
    23941 attgaggtac ggaaaacctt gaatcccatc tctgccatca gggcgatatc ttctttataa
    24001 cgatgataaa aatccgtcgc ctcatggctg ggataaaact cgtcatcgcg caactgaaaa
    24061 cgtttttcca gccccagttt caccgccatt cgatgctcgc cgtgtgggat catatcgacg
    24121 gtggtcagcc cttttccgcc ttcacggaac gcaccttcag actggttggc ggcaagcgcg
    24181 ccgccccata aaaaaccttc tggaaatact gacattttca tcctcaatta agacttactt
    24241 ctttggcgac ggtcggttga tgctttcgcg cctgggcagc cgcttcctca acaggaatat
    24301 cctcaaagcc gagcaacagt gtgaggataa acgacagcac caccgccagc gccatgaccg
    24361 cgaacaccca gacgatgctc attggattcg ccggatcgaa gaattgcacg ctggtaaata
    24421 gccccggcgc tgccattgag tggctggcaa gccccgccat accggcaacc gcgccgcaaa
    24481 taaaaccgct gataagactg gcgataagcg gacgtttcag gcggatcgcc acgccgtata
    24541 acgccggttc ggaaatcccc gccataatgg ctgatgccgc cgcagccagc gccgtctggc
    24601 gcagttccgg gtttttcgtt ttccacggca ccgccagtga tgaaccgccc agcgacaggt
    24661 tagcgccgat ctctgatggc atgaccattc cttctttgcc ggtttcggca atggtctgaa
    24721 tgatggttgg cgtaaagacg cggtgcatcc cggtcattac cagcagaggc cacagcgcgc
    24781 ccataatggc gactgaaagc cagcccagat aaccatgaat ggtgtaaacc agcgccgaaa
    24841 tggcgctacc gatccagata ccaatcgggc caatcagcag gattgccagc ggtgcggcaa
    24901 tcaacacaat cagcattggc ttgaggaagt ttttggtcac cgccggtgta atgcgatcca
    24961 cccagcgttc gatatatgac aggcaccagg tcatcaccag cgccgggatc accgtgtagg
    25021 tgtatttcac cgcggtgacc gggatcaggg caaattcgac atgttcaccc tgggccgctt
    25081 tcgccattaa ttcaataaaa ctcggatgta ccagcacacc cgcgatggca atcgccagcg
    25141 acatattggt tttaaatttg atggcggcag atgccgcgac cattagcggc aggaagaaga
    25201 aagcaccgtc accaatcaca ttcagaatgg ttaaggtcgg cgatcctttt gtcagcacgc
    25261 cgctcatctc gaggatcatc gccagcagtt tgaccatcga tccgccgata atcgccggga
    25321 tcagcggtga catggtgccg atcagcgcat cgaggatccc cgcaccaatg cgacgcagcg
    25381 tgagtttggg tttacccacg ggctgtgcgg gctgcatatc tcccggcagc aggctgacga
    25441 cttcctgaaa ggcctgcgaa accgtattgc cgataattac ctgacactgg ttgtcactac
    25501 gtaccacgcc gagcacgccg gggatggttt ttaacgtcgg gctgtcgata agctggtcat
    25561 ctttgataac aaagcgcaaa cgcgtcatac agtgcgtgac tgccgagatg ttatcaacgc
    25621 cgcccagtgc cgctatcacc gagcgtgcca gcgccgcata atttttggcc atcggatttt
    25681 atcctgttat cagtagggta gatgtagttg ttatcagtcg acgtgacgga aaaataccga
    25741 tgctgaaacg gaattaagaa accggtttca caaaatcatg aatagttgtc actaaataag
    25801 caagagagtt aattgttcat tttttatatt tgtgaaaccg gtcacctgaa tatttttccc
    25861 gccgattgcg caaggacaat cggcaaacgg tgcagtacac tgcgttgatt gcgtgtaaag
    25921 ggatgaaaat gatgacgacg atgctggaag tggcgaagcg cgccggggtt tcaaaagcga
    25981 ccgtttcccg cgtgctttca ggtaatggct acgtcagcca ggagactaaa gatcgcgtgt
    26041 ttcaggcggt agaagagagc agttaccgcc caaatttgct ggcccgcaat ctgtcggcga
    26101 agagtactca gacgctgggg ctggtagtga ccaacacgct ttatcacggc atttatttta
    26161 gtgaattact gtttcatgcc gcgcgaatgg cggaagagaa agggcgacag ttgctattgg
    26221 cagatggtaa acacagcgcc gaagaagagc gccaggcgat tcagtatctg ctggatttgc
    26281 gctgcgacgc cattatgatt tacccgcgtt ttttaagcgt ggatgagatt gatgacatca
    26341 ttgacgcgca cagtcagccg ataatggtgc ttaatcgccg cctgcgcaat aatagcagcc
    26401 atagcgtctg gtgcgatcat aaacagacca gctttaacgc cgtggcagag ttgataaacg
    26461 ccgggcatca ggagattgct ttccttaccg gctcgatgga ttcccccacc agcattgaac
    26521 gtcttgccgg gtataaagac gcgctgtcgc agcatggtat tgcgctcaat gaaaaactta
    26581 tcgctaacgg taaatggacg cccgccagcg gggccgaagg ggtagaaacg ttgctcgaac
    26641 gtggggctaa atttagcgcg ttagttgcca gtaacgacga tatggcgata ggtgcgatca
    26701 aagcgttgca cgagcgcggc gtagcggtgc cagagcaggt gtcagttatc ggattcgatg
    26761 atatcgcaat tgccccc
//
LOCUS       DAJVQH010000044        26672 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJVQH010000044 DAJVQH010000000
VERSION     DAJVQH010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.044"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..2266
                     /locus_tag="OQM87_001936"
                     /pseudo
     CDS             1..2266
                     /locus_tag="OQM87_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2267..3916
                     /locus_tag="OQM87_001937"
     CDS             2267..3916
                     /locus_tag="OQM87_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCS1508089.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3921..4697
                     /locus_tag="OQM87_001938"
     CDS             3921..4697
                     /locus_tag="OQM87_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCS1508090.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDMDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4972..7821
                     /gene="rcsC"
                     /locus_tag="OQM87_001939"
     CDS             4972..7821
                     /gene="rcsC"
                     /locus_tag="OQM87_001939"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCS1508091.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKKTLTVYAERVRKSRDS"
     gene            complement(8021..8671)
                     /gene="rcsB"
                     /locus_tag="OQM87_001940"
     CDS             complement(8021..8671)
                     /gene="rcsB"
                     /locus_tag="OQM87_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCS1508092.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8688..11360)
                     /gene="rcsD"
                     /locus_tag="OQM87_001941"
     CDS             complement(8688..11360)
                     /gene="rcsD"
                     /locus_tag="OQM87_001941"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCS1508093.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQILIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            12098..13219
                     /gene="ompC"
                     /locus_tag="OQM87_001942"
     CDS             12098..13219
                     /gene="ompC"
                     /locus_tag="OQM87_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1508094.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13331..14386
                     /gene="apbE"
                     /locus_tag="OQM87_001943"
     CDS             13331..14386
                     /gene="apbE"
                     /locus_tag="OQM87_001943"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCS1508095.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14460..15524
                     /gene="ada"
                     /locus_tag="OQM87_001944"
     CDS             14460..15524
                     /gene="ada"
                     /locus_tag="OQM87_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCS1508096.1"
                     /translation="MKNATCLTDDQRWQSVLARDPDADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15524..16118
                     /gene="alkB"
                     /locus_tag="OQM87_001945"
                     /pseudo
     CDS             15524..16118
                     /gene="alkB"
                     /locus_tag="OQM87_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            16194..17837
                     /gene="yojI"
                     /locus_tag="OQM87_001946"
     CDS             16194..17837
                     /gene="yojI"
                     /locus_tag="OQM87_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCS1508097.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            18055..18414
                     /locus_tag="OQM87_001947"
                     /pseudo
     CDS             18055..18414
                     /locus_tag="OQM87_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            18648..19679
                     /locus_tag="OQM87_001948"
     CDS             18648..19679
                     /locus_tag="OQM87_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCS1508098.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRTKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQSTLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(19829..20317)
                     /gene="eco"
                     /locus_tag="OQM87_001949"
     CDS             complement(19829..20317)
                     /gene="eco"
                     /locus_tag="OQM87_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCS1508099.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            20725..21219
                     /gene="napF"
                     /locus_tag="OQM87_001950"
     CDS             20725..21219
                     /gene="napF"
                     /locus_tag="OQM87_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCS1508100.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRVWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            21209..21472
                     /gene="napD"
                     /locus_tag="OQM87_001951"
     CDS             21209..21472
                     /gene="napD"
                     /locus_tag="OQM87_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCS1508101.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            21469..23955
                     /gene="napA"
                     /locus_tag="OQM87_001952"
     CDS             21469..23955
                     /gene="napA"
                     /locus_tag="OQM87_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCS1508102.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTAMKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            23962..24657
                     /gene="napG"
                     /locus_tag="OQM87_001953"
     CDS             23962..24657
                     /gene="napG"
                     /locus_tag="OQM87_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCS1508103.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            24644..25507
                     /gene="napH"
                     /locus_tag="OQM87_001954"
     CDS             24644..25507
                     /gene="napH"
                     /locus_tag="OQM87_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCS1508104.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            25504..25953
                     /gene="napB"
                     /locus_tag="OQM87_001955"
     CDS             25504..25953
                     /gene="napB"
                     /locus_tag="OQM87_001955"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCS1508105.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            25963..26565
                     /gene="napC"
                     /locus_tag="OQM87_001956"
     CDS             25963..26565
                     /gene="napC"
                     /locus_tag="OQM87_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCS1508106.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            26584..>26672
                     /locus_tag="OQM87_001957"
     CDS             26584..>26672
                     /locus_tag="OQM87_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein CcmA"
                     /protein_id="HCS1508107.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWV"
BASE COUNT         6360 a         6575 c         7325 g         6412 t
ORIGIN      
        1 aattaacctc gtcgctgaaa ggtaaacctg tttctgcgca gctaacggta ggcgtggtcg
       61 atgaaatgat ctacgcgctg caaccagaaa tcgcgccgaa tatcggcaaa tttttctatc
      121 cgctggggcg taacaatgtg cgtaccagct ccagtctgtc gtttatcagc tatgaccagg
      181 cgctctccag cgagccggtt gcgcctggcg cgactaaccg cagcgagcgg cgagtaaaaa
      241 tgcctgaacg tccacggcgt gaagaggtgg ataccgcggc atggatgccg tcactcacaa
      301 ccgataaaca aggcaaagcg tatttcacgt tcctgatgcc tgattcgtta acccgctggc
      361 gtatcaccgc gcgtgggatg aacggcgacg ggctggtcgg gcaggggcgt gcttatctgc
      421 gttcggaaaa aaatctctac atgaagtgga gtatgccaac ggtgtatcgc gtgggcgaca
      481 aaccggcggc aggactgttt atcttcagtc agcaggataa cgaaccggtg gcgctggtga
      541 ctaaatttgc aggcgctgag atgcgccaga cgctgacgct gcacaaaggg gcgaattata
      601 tttcgctgac gcagaatatt cagcaatctg gcttgttaag tgcagaactg caacaaaatg
      661 ggcaagtgca ggacagcatt agcacaaaac tgtcttttgt ggacaacagc tggcccgttg
      721 aacagcagaa aaatgtcatg ctcggcggtg gcgagaacgc gctgatgttg cccgagcagg
      781 cgagtaatat ccggctacaa agtagtgaaa cgccgcagga gattttccgc aacaatcttg
      841 atgcgttagt cgatgaaccg tggggtgggg tgatcaacac cggtagccgt ctgatcccgc
      901 tcagtctcgc ctggcgttcg cttgccgatc atcaaagtgc cgccgctaac gacattcgtc
      961 agatgattca ggataaccgt ctgcggctga tgcaactggc ggggcccgga gcgcgcttta
     1021 cctggtgggg tgaagatggc aatggtgacg ccttccttac ggcatgggca tggtacgccg
     1081 attggcaggc cagccaggcg ctcggcgtaa cgcaacaacc ggaatactgg cagcatatgc
     1141 tcgacagtta tgccgagcag gcggataaca tgccgttatt gcatcgggcg ctggtgctgg
     1201 cgtgggcaca ggagatgaat ctgccgtgca aaacgttgtt gaaagggggg gatgaagcta
     1261 tcgcccggcg cggaactaaa gatgaagatt tctctgagga agacatccgc gatatcaatg
     1321 acagcctgat cctcgataca ccggaatctc cactggcaga tgcggtggca aacgtcttaa
     1381 ccatgacgtt gctgaaaaaa gcgcagttga agtccacggt gatgccacag gttcagcaat
     1441 atgcgtggga taaagcggca aacagcaatc agccgctggc gcacacggtt gtgctgctca
     1501 atagcggtgg tgacgctacc cagacggccg ctattttaat ggtttgaccg ctgagcaatc
     1561 cactattgag cgcgcgctgg ccatgaactg gctggcgaaa tatatggcga caatgcctcc
     1621 agttgttttg cctgcgcctg cgggcgcatg ggctaaacat aagttaactg gagggggcga
     1681 agactggcgt tgggttggtc agggtgtgcc ggacattctc tcttttggtg acgaattatc
     1741 gccgcaaaat gtgcaggtcc gctggcgtga accggcaaaa acggctcaac aaagtaacat
     1801 tccggtgacc gttgaacgcc agttgtatcg gcttatcccc ggtgaagaag agatgagctt
     1861 tactctgcag ccggtgacca gcaatgagat tgacagcgat gcgctgtatc tcgatgaaat
     1921 tacgcttacc tgcgagcagg atgcagttct gcgctatggt caggtggaag taccgctccc
     1981 gccgggagcc gacgttgagc gcacaacatg gggcatttca gtcaataaac ccaacgccgc
     2041 ggaacagcag gggcaattgc tggaaaaagc gcgtaatgaa atgggcgaac tggcctatat
     2101 ggtgccggtg aaagaactga cgggaacggt cactttccgc catttgctgc gcttctcgca
     2161 aaaagggcaa ttcgttctgc ctcctgctcg ttatgtgcgt tcctatgcac ctgcgcagca
     2221 aagtgtcgcg gcagggagtg aatggaccgg gatgcaggtg aaataagtga actggcgcag
     2281 aatcgtctgg ctattggcgt tggttactct gccaacgctt gcggaggaga cgccgttaca
     2341 actggtgctg cgcggtgcgc agcacgatca actgtatcag ctgtcgtcat caggcgtgac
     2401 caaagtctca gcattacctg actcgctcac cacgccgtta gggagtttgt ggaaactgta
     2461 tgtttacgcc tggctggagg atacccatca accggagcag ccctaccagt gtcgcggaaa
     2521 ctccccggaa gaggtttatt gttgtcaggc tggggagagc ataacgcgcg ataccgcgct
     2581 ggtacgttcc tgtgggctct attttgcgcc gcagcggttg catattggcg cagatgtgtg
     2641 ggggcaatac tggcaacagc gtcaggcacc tgcgtggtta gcgtctctta caacgctgaa
     2701 gccagaaacc tcggtaacgg ttaaatcgct gcttgattca ttagcgacgt tacctgcgca
     2761 aaacaaagct caggaagtct tactggatgt ggtgctggac gaggcgaaaa tcggtgtctc
     2821 atccatgctc ggtagtcggg tacgggtgaa aacctggagc tggtttgccg atgataaaca
     2881 ggaaatccgt cagggtggtt ttgctggctg gttaaccgat ggtacaccgc tgtgggttac
     2941 tggcagtggc accagcaaaa cggtactaac ccgttatgcg acggtgctta atcgggtttt
     3001 gccagtgccg acgcaggttg ccagtgggca atgtgtagag gtggagctgt ttgcccgcta
     3061 tccgctgaag aaaatcacag cggaaaagag tacgacggca gtaaaaccca gcgtacttaa
     3121 tgggcgctat cgtgtgacgt ttactaatgg taaccacatt acgtttgtca gccacggtga
     3181 gacgacctta ctgagcgaaa agggcaaact gaaattgcag tcgcatctcg atcgtgaaga
     3241 gtacgttgcc agagttctgg atcgcgaggc gaaaagtacg ccacctgagg cagcgaaagc
     3301 catgactgtg gcgatacgta cttttttaca gcaaaacgcc aatcgggaag gtgattgcct
     3361 gaccatcccc ggcagcagcg ccacgcagcg tgtttctgct tcgccagcga ccacgggggc
     3421 gcgaacgatg accgcctgga cgcaggagct catctatgca ggcgatccgg ttcattatca
     3481 tggcagtcgc gctaccgaag ggacactttc ctggcggcaa gccacggccc aggcggggca
     3541 gggggagcgt tacgaccaga tactcgcgtt tgcttatccc gacaacagcc ttagccgctg
     3601 gggagctcca cgctcaacct gccagttatt acccaaagcg aaagcctggc tggcgaaaaa
     3661 aatgccgcag tggcggcgta tattacaagg cgagacgggg tacaacgaac cagacgtgtt
     3721 tgcggtctgt cgtttagtct caggtttccc ctataccgat cggcagcaga aacggctgtt
     3781 tattcgtaac ttctttacgc ttcaggatcg gctggattta acccatgagt atctgcactt
     3841 ggcctttgat ggttatccca ccgggcttga tgagaactat atcgaaacgc tgacccgaca
     3901 attattaatg gactaatgct atgcgaaaga tttttcttcc gttgttactg gtggcacttt
     3961 cgcctgtcgc tcacagtgaa ggtgtgcagg aagtcgagat tgacgcaccg ctctctggct
     4021 ggcatccggc agagggcgaa gatgccagtt tttcacaatc gatcaattac ccggcgtcgt
     4081 ccgtcaacat ggcagatgat caaaatatct cagcgcagat cagcggcaaa ataaagaatt
     4141 atgctgcggc gggtaaagtt cagcagggcc ggctggtggt caacggtgcc agtatgccgc
     4201 agagaattga atccgatggt tcatttgcac gcccttatat tttcactgaa ggcagcaaca
     4261 gcgtgcaggt catcagcccg gatgggcaaa gccggcaaaa aatgcagttt tactcaacgc
     4321 cgggtacagg aactattcgt gcacgtttac ggctggttct ctcgtgggat atggacaata
     4381 ccgatctcga tctccatgtt gtcacgcccg atggcgaaca cgcctggtac ggtaacaccg
     4441 tgctgaaaaa cagtggtgca ctggatatgg atgtcacgac ggggtacgga cccgagattt
     4501 tcgccatgcc agcaccagtt cacgggcgct atcaggtgta tatcaactac tatggtgggc
     4561 gcagcgaaac ggaattgacg accgcccaac tgacgctgat caccgatgaa ggatcggtca
     4621 atgagaaaca ggaaacattt attgtaccga tgcgtaatgc tggcgagctg acgctggtga
     4681 aaagttttga ctggtgatcc atcccttcac acaacctggc tctctctttt ttcagataag
     4741 actgctgggc gatctgccga cgccaggata taaccattaa caagcgtcgg cagattggtc
     4801 atttcttatt tttcgtactg agaactgtcc atctactccg gctttcctgg ttaaggcaat
     4861 ggtttctaag cgatacgcat tattgcaatg gtgacagaac attgcggtaa cacgctttta
     4921 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
     4981 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
     5041 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
     5101 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
     5161 caacgcacct ctgatgtgat gaaagagctt aagtacatcg ccgaaaatcg cttatcggca
     5221 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
     5281 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
     5341 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctcaac
     5401 cgggtatttt taatcggcag tgataacctc tgcatggcca atttcggtct gcgtgatatg
     5461 ccagtggaac gcgataccgc gttgaaagcg ttgcatgaac gcatcaataa atatcgaaat
     5521 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
     5581 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
     5641 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
     5701 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
     5761 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
     5821 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
     5881 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
     5941 gtgcttgccg gagctgcatt gtttactctc gcgcggatgt acgagcgacg tattttcatt
     6001 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
     6061 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
     6121 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
     6181 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
     6241 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
     6301 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
     6361 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
     6421 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
     6481 gtcgatcgtc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
     6541 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
     6601 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
     6661 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
     6721 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
     6781 ggctgtatag ttttgcatgt tcgcgctgat ggcgattatc tctctatccg tgttcgcgat
     6841 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtt
     6901 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
     6961 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
     7021 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
     7081 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
     7141 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
     7201 gaagatgtgt taatcactga cgaggtagtg agtaaaaaat ggcagggcag agcggtagtg
     7261 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
     7321 agtgtggctg ctccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
     7381 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataacgac
     7441 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
     7501 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gcgtcgatgc gcttaatgta
     7561 cttagtaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
     7621 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
     7681 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctc
     7741 tcgaagccgg taacgctgga tgtgataaaa aagacgctga cggtatatgc cgagagggtc
     7801 aggaaatcgc gggattcgta ggacggataa ggcgtttacg ccgcatccgg cattcagtgc
     7861 aaatgccaga taagacactg acgcgtctta tctggcctac tttaatgtaa tctgttgaaa
     7921 taatgggaat cgtaggccgg ataaggcgtt tacgccgcat ccggcaatcg gtgcaaatgc
     7981 cagataagac actaacgcgt cttatctggc ctacagatga ttagtcttta tctgccggac
     8041 ttaaggtcac agaagagaga taattcagca gggcgatatc gttctcgacg cccagcttca
     8101 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
     8161 tctcggtcac taggaggcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
     8221 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
     8281 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
     8341 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
     8401 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
     8461 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
     8521 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
     8581 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
     8641 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
     8701 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ttggaacatc cttctcacga
     8761 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
     8821 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
     8881 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
     8941 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
     9001 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
     9061 acgcacaatt gaccaggccc aatttctcgt acgccagact catcatcgct taaaagcaag
     9121 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
     9181 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaacaggcg agtgacaata
     9241 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
     9301 tcttcctctt caacttccgg gtcagcggcg agcattttga tatgcacaga gtagcgtgta
     9361 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
     9421 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
     9481 aacgggaagt gcaaattatc catttcatga atacttacgc cttctcccgt gtccagaatg
     9541 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
     9601 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
     9661 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
     9721 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
     9781 aacagcaccg tctcactttt ccagctatca tccgcaagca tgttcgctaa ctgtatttct
     9841 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
     9901 agtttagccg cactctccgc cagggactgt gcgggttctt tcagcgcatc gccaatgttt
     9961 ttcataaagg tcatccgccc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    10021 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgagaatttg tgtgcgcggc
    10081 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    10141 atcccctgat gctgttccgc catggtggtg atgttttgca gattcagatg cggcagcaaa
    10201 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    10261 ccgagcggca acagtgagac tatctcttca ttaattgccc gtaaaatgcg caattcgtta
    10321 ttgaccgccg tgctggggac gctttctgta ctgcggctgg agaaatggcg gaatgtggta
    10381 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    10441 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    10501 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    10561 gtcccttctt tctcattatc attgtttccc gttgccgtcg cgtctggctc aaggcggaaa
    10621 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    10681 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    10741 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    10801 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    10861 gtgagatctt taaggggtag ggttgagatc agcaccagac tgttatcctg accattcagg
    10921 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    10981 cgctgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    11041 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    11101 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgcctggt cacgtaacgc
    11161 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    11221 ttcagccagg cgttaacggc actttgtacc attaccccca tcgtcaccag taacacaatg
    11281 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    11341 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    11401 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    11461 cccgtgatat ttatcatcct gtatgggggg aaatagatgt acaagcgcca ttttgctgta
    11521 caaataacct acaaaaagcc caacgaaatt aattgtaaat aaacaaagat tgctggaaat
    11581 tatgctgatg ttaattattt gtgaaatagt taacaagcgt tatagttttt ctgtggtagc
    11641 acagaataat gaaaagtgtg taaagaaggg taaaaaaacc gaatgcgagg catccggttg
    11701 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    11761 agcgggagtt attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    11821 cttaagaata agttattgat tttaaccttg aattattatt gcttgatgtt aggtgcttat
    11881 ttcgccattc cgcaataatc ttaaaaagtt cccttgtatt tacattttga aacatctgta
    11941 gcgataaatg aaacatctta aaagttttag tatcatattc gtgttggatt attctgcatt
    12001 tttggggaga atggacttgc cgactgatta atgagggtta atcagtatgc agtggcataa
    12061 aaaagcaaat aaaggcatat aacagagggt taataacatg aaagttaaag tactgtccct
    12121 cctggtccca gctctgctgg tagcaggagc agcaaacgct gctgaagttt acaacaaaga
    12181 cggcaacaaa ttagatctgt acggtaaagt agacggcctg cactatttct ctgacgacaa
    12241 gtctgtagat ggcgaccaga cctacatgcg tcttggcttc aaaggtgaaa ctcaggttac
    12301 tgaccagctg accggttacg gccagtggga atatcagatc cagggcaaca gcgctgaaaa
    12361 cgaaaacaac tcctggaccc gtgtggcatt cgcaggtctg aaattccagg atgtaggttc
    12421 tttcgactac ggtcgtaact acggcgtagt ttacgacgta acttcctgga ccgacgtact
    12481 gccagaattc ggtggtgaca cttacggttc tgacaacttc atgcagcagc gtggtaacgg
    12541 cttcgcaacc taccgtagca ccgacttctt tggtctggtt gacggtctga acttcgctgt
    12601 tcagtatcag ggcaaaaacg gtagcccgga aggtgaaggc atgaccaaca acggtcgtga
    12661 agctctgcgt cagaacggtg acggcgttgg cggttctatt acttatgatt acgaaggctt
    12721 cggtatcggt gctgcagttt ccagctccaa acgtactgat gaccagaact tcggtctgaa
    12781 ccgttatgat gagcgttaca tcggtaatgg cgaccgcgct gaaacctaca ccggtggtct
    12841 gaaatacgac gcgaacaaca tctacctagc tgctcagtac acccagacct acaacgcaac
    12901 tcgcgtaggc aacttgggtt gggcgaacaa agcacagaac ttcgaagctg ttgctcagta
    12961 ccagttcgac ttcggtctgc gtccgtccct agcttacctg cagtctaaag gtaaaaacct
    13021 gggtgtcatc aatggtcgta actacgacga cgaagatatc ctgaaatatg ttgatgttgg
    13081 tgcgacctac tacttcaaca aaaacatgtc cacctacgtt gactacaaaa tcaacctgct
    13141 ggacgacaac cagttcactc gtgacgctgg catcaacact gataacatcg tagctctggg
    13201 tctggtttac cagttctaat ctcgatggat atcgaacaaa gggcctgcgg gccctttttt
    13261 cattgttttc agcgtacaaa cacagttttt tggtgtactc ttgcgaccgt tcgcatgagg
    13321 ataatcacgt atggaaataa gctttacccg cgtggcactg ctggttgccg cgctcttctt
    13381 tgttggttgc gatcaaaaac cacaacccgc caaaacccac gctactgaag ttaccgttct
    13441 tgaaggcaaa accatgggta ccttctggcg tgccagcatc ccgggcattg atgccaaacg
    13501 cagtgccgaa cttaaagaaa agattcagac ccagctggac gccgacgatc agctgctttc
    13561 gacctataaa aaagattccg cgctgatgcg ctttaacgac tcgcaaagtt tgtcgccgtg
    13621 gccggtaagt gaagcgatgg ccgatatcgt caccacctcg ctgcgcattg gggcaaggac
    13681 cgatggtgcg atggatatta ccgtcgggcc gctggtgaat ctgtggggct ttggcccgga
    13741 acaacagccg gttcaaattc cgagccagga acagatcgat gcgatgaaag ccaaaaccgg
    13801 cttacagcac ctgacggtca tcaatcagtc gcatcagcaa tatctgcaaa aagacctgcc
    13861 ggatttatat gtcgatctct ccaccgtcgg tgaaggttat gcggcggatc acctggcacg
    13921 cttgatggag caggaaggga tttcccgcta tctggtgtcg gtgggcggcg cgctgaacag
    13981 ccgtggtatg aacggtgaag gccagccgtg gcgggtagcg attcaaaaac caaccgataa
    14041 agaaaacgcg gttcaggctg tggtggatat caacggtcat gggattagca cttctggcag
    14101 ctaccgtaac tattacgaac tggacggcaa acgtctttcc catgttatcg atccgcaaac
    14161 cgggcgtccc atcgaacaca atctggtatc cgtgacggtg attgctccga cggcgctgga
    14221 agccgatgcc tgggatactg gcttgatggt actcgggccg gagaaagcca aagaggttgt
    14281 tcgccgggaa gggctggcgg tttatatgat caccaaagaa ggcgatagct ttaaaacctg
    14341 gatgtcacca cagtttaaaa gcttccttgt cagcgaaaaa aattaaagcg caagattgtt
    14401 ggtttttgcg tgatggtgac cgggcagact aaaggctatc cttaaccagg gagctgatta
    14461 tgaaaaacgc cacatgctta actgacgatc aacgctggca atctgtctta gcccgcgacc
    14521 cggatgccga cggcgaattc gttttcgccg tgcgcaccac gggtatcttt tgccgtccgt
    14581 cttgccgcgc cagacatgcc ttgcgggaaa atgtctcctt ctacgcaaat gccagcgagg
    14641 cactcgccgc cggatttcgc ccctgcaaac gttgtcagcc agacaaagcc aatccccggc
    14701 aacatcggtt ggataaaatc acccacgcgt gtcgactgct ggaacaggaa acgcctgtaa
    14761 cgctggaagc cttagccgac caggtggcga tgagtccatt tcatctacat cggttgttta
    14821 aagctactac cggaatgacg cctaaagcct ggcaacaggc ctggcgcgct cgccgtttgc
    14881 gcgaatcgct ggcgaaaggg gagagcgtga cgacgtctat tcttaacgcc ggattccccg
    14941 acagcagcag ttactaccgc aaagccgatg aaacgctggg catgacggct aaacaattcc
    15001 gtcacggtgg cgaaaatctg gcggtgcgtt acgcgctggc tgattgtgag ctgggtcgtt
    15061 gcctggtggc agaaagcgag cgggggattt gcgcgatatt gctgggcgat gatgacgcga
    15121 cattaatcag cgagttgcag cagatgtttc ccgctgccga caacgcgcct gccgatctga
    15181 cgtttcagca acatgtgcgt gaagtgatcg ccagcctcaa tcaacgcgat acgccgctga
    15241 cgttaccgct ggatattcgc ggcactgctt ttcagcaaca agtctggcag gcactgcgca
    15301 cgataccttg tggtgaaacc gtcagttatc agcaactggc taacgccatc ggcaaaccga
    15361 aagcggtacg ggccgttgcc agcgcctgtg ccgccaacaa gctggctatc gtaataccct
    15421 gtcatcgagt ggtccgtggt gatggcacac tttccggtta ccgctggggc gtgtcgcgta
    15481 aagcgcaact gctgcgccgc gaagctgaaa atgaggagag gtaatgttgg atctgtttgc
    15541 cgatgctgaa ccgtggcaag agccactggc ggctggtgcg gtaattttac ggcgttttgc
    15601 ttttaatgct gcggagcaac taatccgcga tattaatgac gttgccagcc agtcgccgtt
    15661 tcgccagatg gtcgctccag gcggatatac catgtcggtg gcgatgacca actgtgggca
    15721 tctgggctgg acgacccatc ggcaaggtta tctctattcg cccattgatc cgcaaacaaa
    15781 taaaccgtgg cccgccatgc cacaaagctt tcatgattta tgtcaacgtg cggctacggc
    15841 ggcgggctat ccagatttcc agccagatgc ttgtcttatc aaccgctacg ctcctagcgc
    15901 gaaactgtcg ctgcatcagg ataaagacga accggatctg cgcgcgccaa ttgtttctgt
    15961 ttctctgggc ttacccgcga tttttcaatt tggtggcctg gggcggtgaa tcgcggctgt
    16021 tttatcacgg tattcaaccg ttgaaagcgg ggtttcatcc actcaccacc gactgccgct
    16081 acaacctgac attccgtcag gcaggtaaaa aagaataaaa ataagaatta ttattgctgt
    16141 gcgcgcgaag atgtttaaac tgcgggctgt aattcattgt ccgggttttc tgcatggaac
    16201 ttcttgtact tgtctggcgg cagtatcgct ggccatttat cagtgtgatg gcgctaagcc
    16261 tcgccagtgc ggcattaggc attggcttaa ttgcttttat caatcagcgc cttatcgaaa
    16321 cggcggatac cagtctgctg gtgttaccgg agtttctggg attattgctg ctgttgatgg
    16381 cagtcactct cggatcgcaa ctggcgctca ccactttggg gcatcacttc gtttaccgac
    16441 tgcgtagtga atttatcaag cggattctgg atacccacgt cgagcgcatt gaacaactcg
    16501 gtagcgcctc gttgctggcg ggattaacca gcgatgtgcg caatatcacc attgcttttg
    16561 tgcgtctgcc ggaactgggg caggggatca ttctcactat cggttcatcg gcgtatctgt
    16621 ggatgctgtc gggcaaaatg ttgctggtaa cggctatctg gatggcgatc accatctggg
    16681 gcggttttgt gctggtggcg cgggtgtaca aacatatggc gaccctacgt gaaaccgaag
    16741 acaagctgta cacggatttt caaactgtac ttgaagggcg caaagagctg actctgaacc
    16801 gggaacgcgc cgagtatgtg tttaacaacc tctacatccc cgatgcgcaa gagtatcgcc
    16861 atcatattat tcgcgcagac accttccatc ttagtgccgt gaactggtca aacatcatga
    16921 tgctgggcgc tatcggcctg gtgttctgga tggcgaacag tctcggttgg gctgatacca
    16981 acgttgctgc gacctattcg ttgacgctgt tattcctgcg tacgccgctg ctttcggcgg
    17041 ttggcgcatt gccgacgctg ctgacggcgc aggtggcgtt taacaagctg aacaaattcg
    17101 cgctcgcgcc tttcaaagct gagtttccgc gcccgcaggc gtttcccaac tggcaaacgc
    17161 tggagctgcg taacgtgacg tttgcttatc aggataacgc gttttccgtt ggtccgatta
    17221 atctcaccat caaacgtggc gagctgctgt ttctgattgg cggcaacggt agcggaaaat
    17281 cgacgctggc gatgttgttg acgggcttgt atcagccaca aagcggcgaa atcttgctgg
    17341 atggcaaacc tgtcagcggc gaacaaccgg aagattatcg caaactgttt tcggcagtgt
    17401 ttaccgatgt ctggctgttt gatcaactgc tggggccgga ggggcaaccc gctaacccgc
    17461 aactggttga gaagtggctg gcgcagctga aaatggctca taagcttgag ttaagcaacg
    17521 ggcgtattgt taacctgaag ttatcaaaag ggcagaaaaa acgcgtgccg ctgttgctgg
    17581 cgctggcaga agaacgcgat attatcctgc tggatgaatg ggcggcggat caggatccac
    17641 acttccgtcg tgagttttat caggtgttgc tgccgctgat gcaggagatg ggtaaaacta
    17701 ttttcgctat cagtcatgat gatcattact ttatccacgc cgaccgcctg ctggaaatgc
    17761 gcaatgggca acttagcgag ctgacgggcg aagagcgcga tgccgcttcg cgtgatgccg
    17821 ttgcccggac ggcataatat tacgacgctg acctggcgga ctgctgccgt caggtcaata
    17881 tgctaaaaat cccttcattt ctaataaccc tataattaat caacgaaatt ataatgtttc
    17941 taaaattaga atataattta taaacattat ttaaatgttg ttacttaagt gttaccgttg
    18001 atgccgcgca aatctcacct gcaataaagc gactaaaagt aaggcattaa caagatgaaa
    18061 aaagtgactg ccatgctctt ctcgatggcc gtggggctta atgccgtttc gatggcggca
    18121 aaagcgaaag cgtccgagga gcaggaaact gatgtactgt tgattggcgg cggcattatg
    18181 agcgccacgt tggggaccta tttacgcgag ctggagcctg aatggtcgat gaccatggtg
    18241 gagcgcctgg aggggggcgc gcaggagagt tcgaacggct ggaacaacgc cggaaccggg
    18301 cattctgcac tgatggaact gaactacacc ccgcaaaacg ccgatggcag catcagtatt
    18361 gaaaaagcag tcgccattaa cgaagcattt cagatttccc gccagttctg ggcgcaccag
    18421 gtcgagcgcg gcgtgctgcg tactccgcgt tcatttatca ataccgttcc gcatatgagc
    18481 tttgtctggg gcgaggataa cgttaatttc ctgcgcgccc gttacgccgc gttgcaacaa
    18541 agctcgctgt ttcgcggtat gcgttactct gaagatcacg cgcagatcaa agagtgggca
    18601 ccgttagtga tggaagggcg cgatccgcaa cagaaattgg cactgatgtg aactatggtg
    18661 agatcacccg ccagttaatt gcttccttac agaagaaatc taacttctcg ctgcaactca
    18721 gcagcgaagt ccgcgcccta aagcgtaatg acgataacac ctggaccgtt accgttgccg
    18781 atctgaaaaa cggcactgca cagaacatcc gcaccaaatt tgtctttatc ggcgcgggcg
    18841 gtgcggcgct gaagctgtta caggaatcgg ggattccgga agcgaaagac tacgccggtt
    18901 tcccggtggg cggacagttc cttgtttcgg aaaatccaga cgtggttaat cgccatctgg
    18961 cgaaagttta cggtaaagca tccgttggcg caccaccgat gtcggttccg catatcgata
    19021 cccgtgttct ggacggtaaa cgcgtagtgc tgtttgggcc atttgccacc ttctcaacca
    19081 aattcctcaa aaacggttca ttgtgggatc tgatgagttc caccaccacc tctaacgtga
    19141 tgccgatgat gcacgtcggg ctggataatt tcgatctggt gaaatatctg gtgagtcagg
    19201 tgatgttgag tgaagaggat cgttttgaag cgttgaaaga gtactatccg caagcgaaaa
    19261 aagaggactg gcgtttgtgg caagcggggc agcgtgtgca gattatcaag cgtgatgccg
    19321 agaaaggtgg cgtactgcgt ctgggtaccg aagtggtcag tgaccagcaa gggaccattg
    19381 cggcgctgct gggggcatcg ccaggggcgt caaccgccgc gccgattatg ttgaatctgc
    19441 tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg gcagtctacg ttgaaagcga
    19501 tcgttccgtc ttatggacgc aagctgaacg gtgatgtagc ggcaacagaa cgcgagttgc
    19561 agtacaccag cgaagtgctg gggctgaaat acgacaaacc gcaagcagca gatagtacgc
    19621 cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt ggcggatatt gcgttgtaat
    19681 gatgtgccac atccggcatg gtatgccgga tgtggcgtat actgataaga cgcgccagcg
    19741 tcgcatcagg caaccggctc gggcgttaga tgttggatgc ggcgtaaccg ccttatccaa
    19801 ccaacggggt gccgtacttc acaacagttt agcgaacttc cgcgttgtca attttctctt
    19861 ccgccttcca gacgcggtat ttcacatcta cattgtctgg cgtatacacc acgatcggca
    19921 gcttgctgtt gtaacgcagc attccagcat cgcccagata cgcggtgaca aatttcttct
    19981 ctttcttgcc atccgggcag gccatcatcg tggaaaccgg cgagctaact ttatcaaaga
    20041 cataataatc atagccccag ccttccagcg ttttgctttc cagcttcccg ccgagacgat
    20101 gcaaattgca atcgacttcc agcgtctgac cgattaacag ttctactttc agggtagatt
    20161 catcttcttg cggggttaac tgaatcacct gacgcttcat gcctgtttca gcttgtggat
    20221 aaggcgcgat tttttccagt ggctggacgc tttctgccgc ccaggcggaa gtggtagcga
    20281 aagcggcaaa caatactgca ggtagaatgg tcttcatttt tttgctttcc ttcttcattt
    20341 ataggttttc accgatgatg tcgctgaagg ttaacatatt catcagattt attaatttgc
    20401 tttatgaata acttaaagaa cattcagaca attttacctg ctcttttagt gttaaattct
    20461 gatgagggag caaacaagca gaaatatctc tggtgaatta atcgctatat aaatatgttt
    20521 ataaccattt gaaatgtgag caaaagcccg tttttgccac gcttcccgcc actcttttga
    20581 tcctgctaca ggttttaccc cgatcggggt atgcatcttt gacacatcct ttaatatctt
    20641 agcggctata aaaatggctt attaattatg cggtctattt ggttgctctc aattttcaga
    20701 gcgcgttaat gatggaaggt caatgtgaag attgatgcat cccgtcgggg catactcact
    20761 ggtcgctggc gcaaagccag taacggtatc cgtccgccct ggtccggtga tgaatctcat
    20821 tttctgaccc attgcacccg ctgcgacgcc tgtattaatg cttgtgaaaa caacattctg
    20881 caacgcggca cgggtggcta tccgagcgtt aatttcaaaa ataacgagtg cagcttctgt
    20941 tacgcctgcg cgcaggcttg ccccgaatcg ctattttctc cgcgccacac cagggtctgg
    21001 gatttgcagt tcaccatcgg ggacgcgtgc ctggcgtatc agtcagttga atgccgccgc
    21061 tgtcaggata gctgtgagcc aatggcaatt atctttcgcc cgacgctctc cgggatctac
    21121 caggcgcaac ttaatagcca actttgcaac ggatgtggag catgtgcagc cagctgtccg
    21181 gtatcagcca taactgcgga gtatctccat gcacactaac tggcaagttt gcagcctggt
    21241 cgtgcaggcc aaaagcgaac gaatttcaga catcagcacc caactgaacg cctttcccgg
    21301 ctgtgaagtt gctgtcagcg acgcgccgag cggtcagttg attgtggtgg tggaagcaga
    21361 agacagcgaa acgctgatcc aaaccattga gtcagtacgc aacgtagagg gcgtgctggc
    21421 ggtgtcgctg gtttatcacc agcaggaaga gcaaggtgag gaaacaccat gaaactcagt
    21481 cgtcgtagct ttatgaaagc taacgccgtt gcggccgctg cggcggctgc cggtctcagc
    21541 gtgccgggcg ttgcccgcgc cgttgttggt cagcaggaag ccattaaatg ggataaagcg
    21601 ccgtgccgtt tctgcggtac tggttgcggc gttctggtcg gaacgcagca ggggcgtgtg
    21661 gtggcctgtc agggcgaccc ggacgcaccg gttaaccgtg gcctgaactg cattaagggc
    21721 tatttcctgc ccaagatcat gtacggtaaa gaccgtttga cgcagccgct gctgcgtatg
    21781 aaaaacggta aatatgacaa agaaggcgaa tttaccccaa tcacctggga tcaggccttc
    21841 gatgtgatgg aagagaaatt caaaaccgcc atgaaagaaa aagggccgga atcgatcggt
    21901 atgttcggtt ctggtcagtg gactatctgg gaaggttatg ccgcgtccaa gctgttcaaa
    21961 gcgggcttcc gttcgaacaa catcgacccg aacgcacgtc actgtatggc gtcggcagta
    22021 gttggcttta tgcgtacctt tggtatggat gagccgatgg gctgctatga cgacatcgag
    22081 caggctgacg cgtttgtgct gtggggcgct aacatggcgg agatgcaccc gatcctctgg
    22141 tcacgcatca ctaaccgtcg tctctctaac cagaacgtca ccgtggcggt gctttctacc
    22201 taccagcatc gtagcttcga gctggcggat aacggcatca tctttacgcc gcaatctgac
    22261 ctggtgatcc tgaactacat cgccaactat atcattcaaa acaatgcgat aaatcaggac
    22321 ttcttcagca agcacgttaa cctgcgcaaa ggggcgacgg acatcggcta cggtttacgt
    22381 ccgacccatc cgctggaaaa agcagcgaag aatccgggtt ctgacgcctc cgaaccgatg
    22441 agctttgaag attacaaagc cttcgttgct gagtatacgc tggaaaaaac cgctgaaatg
    22501 accggcgtgc cgaaagacca gttagaacaa ctggcgcagc tgtatgccga tccgaacaag
    22561 aaagtcatct cctactggac gatgggcttc aaccagcata cccgtggtgt gtgggccaac
    22621 aacctggtct acaacctgca cctgctgacc ggcaaaattt cccagccggg ttgcggtccg
    22681 ttctccctga ccgggcaacc ttctgcgtgt ggtactgcgc gtgaagtggg cacctttgct
    22741 caccgtctgc ctgcggacat ggtggtaact aacgagaaac atcgtgatat ctgtgagaag
    22801 aagtggaata tcccgagcgg caccattccg gcgaaaatcg gtcttcatgc ggtggcacaa
    22861 gaccgtgcgc tgaaagacgg caagctgaat gtttactgga ccatgtgtac caacaacatg
    22921 caggccgggc cgaacatcaa tgaagagcgt atgccgggct ggcgcgatcc gcgcaacttc
    22981 atcatcgtct ccgatccgta tccgacagtc agtgcgctgg ccgccgactt gatcctgccg
    23041 accgcaatgt gggtagagaa agagggcgct tacggtaacg ccgaacgccg tactcagttc
    23101 tggcgtcagc aggtacaggc gccaggcgaa gcgaaatcgg atctctggca gttagtccag
    23161 ttctcccgcc gcttcaaaac tgaagaagta tggccggaag agctgctggc gaagaaaccg
    23221 gaactgcgtg gcaaaacgct gtacgaagtt ctgtatgcca cccccgaagt gagcaaattc
    23281 ccggtatccg aactggcgga agatcagctg aacgatgaat cccgcgagct gggcttctat
    23341 ctgcaaaaag ggctgttcga agagtacgca tggtttggtc gcggtcacgg tcacgatctg
    23401 gcaccgttcg atgactacca caaagcgcgc ggtctgcgct ggccggtggt gaatggtaaa
    23461 gaaacacagt ggcgttacag cgaaggtaac gacccgtacg tgaaagcggg cgaaggctat
    23521 aagttctacg gtaaaccgga tggcaaagcg gtgatcttcg cactgccgtt cgaaccggcg
    23581 gcggaagcac cggatgaaga gtacgacctg tggctctcta ccggacgcgt tctggagcac
    23641 tggcacaccg gcagtatgac tcgccgtgtg ccggaactgc accgcgcctt cccggaagcg
    23701 gtcttgttta ttcacccgct ggatgcgaaa gcgcgcgatc tgcgccgtgg cgacaaagtg
    23761 aaagtggttt ctcgccgtgg cgaagtgatc tcgattgttg aaacgcgcgg tcgtaaccgt
    23821 ccgccacagg gcctggtgta catgccgttc ttcgacgccg cacagctggt taacaaactg
    23881 acgctggatg cgaccgatcc gctctcgaaa gagacggact tcaagaagtg cgcggtcaaa
    23941 ctggagaagg tgtaagccat tatgtcccgg tcagcgaaac ctcaaaatgg tcgccgccgc
    24001 tttctgcgcg atgtcgttcg cacagcaggc gggctggctg ccgtgggtgt ggcgctgggg
    24061 ttacaacagc aaaccgcacg cgcatctggc gtgcggttgc gcccgcccgg agccataaac
    24121 gagaacgcct ttgccagtgc ctgtgtgcgt tgcggtcagt gtgttcaggc ttgcccttac
    24181 gacaccttaa aactggcgac gctggcctct ggtctgtcgg cgggcacgcc atattttgtc
    24241 gcacgggata ttccttgcga aatgtgtgag gacattccgt gcgccaaagt gtgcccaagc
    24301 ggtgcgctgg atcgtgagat tgaatcgatc gacgacgcgc ggatggggct ggcggtactg
    24361 gtggaccagg aaaactgtct caactttcag gggctgcgct gcgatgtttg ttatcgcgaa
    24421 tgcccgaaaa ttgatgaggc catcaccttg gagctggagc gcaacacgcg taccggtaag
    24481 cacgcccgct ttctgccgac ggttcacagc gacgcctgta ccggttgcgg taagtgcgaa
    24541 aaagtatgcg tgctggaaca accggcaatc aaggtgttac cgctgtcact ggcgaaaggg
    24601 gagttaggtc accattaccg cttcggctgg ctggagggga acaatggcaa atcgtaaacg
    24661 tgacgccggg cgcgaggcgc tggagaaaaa aggctggtgg cgcagtcacc gttggctggt
    24721 gttacgtcgc ctttgtcagt tcttcgtgct ggggatgttt ttgagcggtc catggtttgg
    24781 tgtgtggatc ttgcacggca actacagcag tagcctgtta ttcgacaccg ttccgctgac
    24841 cgatccgctg atgacgctgc aaagtcttgc cagtggtcat ctgcctgcca cggtggcgtt
    24901 gaccggtgcg gtgattatca ccgtgctcta cgccttggcg ggaaagcgat tattttgcag
    24961 ctgggtctgc ccgctgaacc cgattaccga cttagcgaac tggttacgca ggcgctttga
    25021 cctcaatcag tctgcgacga tccctcgcca cattcgctac gtgctgttgg tggtcattct
    25081 ggtgggatca gccttaactg gcacgctcat ttgggaatgg atcaaccccg tttctctgat
    25141 ggggcgtagc ctggtaatgg gcttcggcag cggcgcgctg cttattctcg cgctgttttt
    25201 atttgatcta ctggtcgttg aacacggctg gtgcgggcac atttgtccgg ttggcgcgct
    25261 gtatggcgtg ctgggtagca aaggtgtgat taccgttgcg gccagcgatc ggcagaaatg
    25321 taaccgctgt atggattgtt ttcatgtttg cccggaaccg catgtgctac gtgccccggt
    25381 gctggatgaa caaagcccgg tgcaggtcac cagccgcgat tgcatgacgt gcggtcgctg
    25441 cgtggatgtc tgttctgagg atgtatttac aataactaca cgatggagtt cgggagcgaa
    25501 atcatgaaaa gccatgacct gaagaaagcg ctgtgtcaat ggacggcgat gctggccctg
    25561 gtggtaagcg gcgcggtttg ggcggctaat ggcgtcgatt ttagccagtc gccggaagta
    25621 tccgggacgc aggaaggggc cattcggatg ccaaaagagc aggatcggat gccgctgaac
    25681 tatgtgaatc agccgccaat gatcccgcat agcgttgaag gttatcaggt aacgaccaat
    25741 accaatcgct gcttgcagtg ccacggtgtc gaaagctatc gcaccactgg cgcgccgcgt
    25801 atcagtccta ctcactttat ggacagcgac ggcaaagtgg gcgcggaagt ggctccgcgt
    25861 cgttatttct gtctgcaatg tcacgtaccg caggccgata ccgcgccaat cgtggggaat
    25921 acctttaccc catcaaaagg ttacgggaaa taagaggtta ttatgggaaa ttctgaccgt
    25981 aagcctggtc tgattaagcg cctgtggaaa tggtggcgta cccccagcca tctggcgctg
    26041 gggacgctgc tgttgatcgg ttttgttggc ggcatcgtct tctggggggg ctttaacacc
    26101 gggatggaaa aagccaatac cgaagagttc tgcattagct gccacgaaat gcgcaacacg
    26161 gtgtatcagg aatacatgga ttccgtgcac tacaacaacc gtagcggcgt ccgtgcgacc
    26221 tgtccggatt gtcacgttcc gcacgagttt gtgccgaaga tgatacgcaa gctcaaagca
    26281 agtaaagagc tgtatggtaa aatttttggc gttattgaca cgccgcagaa atttgaagct
    26341 catcgtctga cgatggcaca gaatgagtgg cggcgcatga aggacaataa ctcgcaggag
    26401 tgccgtaact gtcacaactt cgagtatatg gatacaaccg cccagaaatc ggttgccgcg
    26461 aagatgcatg accaggcggt gaaagatggg caaacctgta ttgattgcca taaagggatc
    26521 gcgcacaagc tgcccgatat gcgtgaagtc gagccaggtt tttaacaggg ttattgcgtg
    26581 ggtatgcttg aagccagaga ggtactttgt gagcgggatg aacgaacctt atttagtggc
    26641 ttgtcattta cgctgaacgc aggagagtgg gt
//
LOCUS       DAJVQH010000045        26047 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJVQH010000045 DAJVQH010000000
VERSION     DAJVQH010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.045"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            164..1147
                     /locus_tag="OQM87_001958"
     CDS             164..1147
                     /locus_tag="OQM87_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1508108.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1170..2681
                     /locus_tag="OQM87_001959"
     CDS             1170..2681
                     /locus_tag="OQM87_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1508109.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2706..3704
                     /locus_tag="OQM87_001960"
     CDS             2706..3704
                     /locus_tag="OQM87_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1508110.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3737..4831
                     /locus_tag="OQM87_001961"
     CDS             3737..4831
                     /locus_tag="OQM87_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1508111.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGYRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4843..5715
                     /locus_tag="OQM87_001962"
     CDS             4843..5715
                     /locus_tag="OQM87_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1508112.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5763..6185)
                     /locus_tag="OQM87_001963"
     CDS             complement(5763..6185)
                     /locus_tag="OQM87_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1508113.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6282..7484)
                     /locus_tag="OQM87_001964"
                     /pseudo
     CDS             complement(6282..7484)
                     /locus_tag="OQM87_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7494..8306)
                     /gene="hcaB"
                     /locus_tag="OQM87_001965"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(7494..8306)
                     /gene="hcaB"
                     /locus_tag="OQM87_001965"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCS1508114.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8303..8623)
                     /locus_tag="OQM87_001966"
     CDS             complement(8303..8623)
                     /locus_tag="OQM87_001966"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCS1508115.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8623..9141)
                     /locus_tag="OQM87_001967"
     CDS             complement(8623..9141)
                     /locus_tag="OQM87_001967"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCS1508116.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(9138..10499)
                     /locus_tag="OQM87_001968"
     CDS             complement(9138..10499)
                     /locus_tag="OQM87_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCS1508117.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10635..11525
                     /gene="hcaR"
                     /locus_tag="OQM87_001969"
     CDS             10635..11525
                     /gene="hcaR"
                     /locus_tag="OQM87_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCS1508118.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11685..12824
                     /locus_tag="OQM87_001970"
     CDS             11685..12824
                     /locus_tag="OQM87_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCS1508119.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12816..14096)
                     /gene="csiE"
                     /locus_tag="OQM87_001971"
     CDS             complement(12816..14096)
                     /gene="csiE"
                     /locus_tag="OQM87_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCS1508120.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRITRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTPQQEQIRKMLESA"
     gene            complement(14287..15141)
                     /locus_tag="OQM87_001972"
     CDS             complement(14287..15141)
                     /locus_tag="OQM87_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1508121.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMLPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15286..16089)
                     /gene="suhB"
                     /locus_tag="OQM87_001973"
     CDS             complement(15286..16089)
                     /gene="suhB"
                     /locus_tag="OQM87_001973"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCS1508122.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGDL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            16208..16948
                     /gene="trmJ"
                     /locus_tag="OQM87_001974"
     CDS             16208..16948
                     /gene="trmJ"
                     /locus_tag="OQM87_001974"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCS1508123.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17218..17706
                     /gene="iscR"
                     /locus_tag="OQM87_001975"
     CDS             17218..17706
                     /gene="iscR"
                     /locus_tag="OQM87_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCS1508124.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17818..19032
                     /gene="iscS"
                     /locus_tag="OQM87_001976"
     CDS             17818..19032
                     /gene="iscS"
                     /locus_tag="OQM87_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCS1508125.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            19060..19446
                     /gene="iscU"
                     /locus_tag="OQM87_001977"
     CDS             19060..19446
                     /gene="iscU"
                     /locus_tag="OQM87_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCS1508126.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19463..19786
                     /gene="iscA"
                     /locus_tag="OQM87_001978"
     CDS             19463..19786
                     /gene="iscA"
                     /locus_tag="OQM87_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCS1508127.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19882..20397
                     /gene="hscB"
                     /locus_tag="OQM87_001979"
     CDS             19882..20397
                     /gene="hscB"
                     /locus_tag="OQM87_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCS1508128.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20414..22264
                     /gene="hscA"
                     /locus_tag="OQM87_001980"
     CDS             20414..22264
                     /gene="hscA"
                     /locus_tag="OQM87_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCS1508129.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22266..22601
                     /gene="fdx"
                     /locus_tag="OQM87_001981"
     CDS             22266..22601
                     /gene="fdx"
                     /locus_tag="OQM87_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCS1508130.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22613..22813
                     /gene="iscX"
                     /locus_tag="OQM87_001982"
     CDS             22613..22813
                     /gene="iscX"
                     /locus_tag="OQM87_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCS1508131.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22872..24155
                     /gene="pepB"
                     /locus_tag="OQM87_001983"
     CDS             22872..24155
                     /gene="pepB"
                     /locus_tag="OQM87_001983"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCS1508132.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKRVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24297..25073
                     /gene="sseB"
                     /locus_tag="OQM87_001984"
     CDS             24297..25073
                     /gene="sseB"
                     /locus_tag="OQM87_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCS1508133.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDCALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25568..>26047)
                     /gene="sseA"
                     /locus_tag="OQM87_001985"
     CDS             complement(25568..>26047)
                     /gene="sseA"
                     /locus_tag="OQM87_001985"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCS1508134.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
BASE COUNT         6168 a         6517 c         7223 g         6139 t
ORIGIN      
        1 caagcacctt aaaatcacca tcatacacta aatcagtaag ttggcagcat taccctacgt
       61 tcactaaacc ctattaactg taatatttga gcggcacgcg ctgccgctca tcacaaaata
      121 attagaataa tccgttgtta ataaaaaccc tacaggaggc actatgccta aaaaaatgag
      181 aacaacccgt aatctattat taatggctac gctattaggt agcgcactat ttgccagggc
      241 tgcggataaa gaaatgacca ttggtgcaat ataccttgat acccagggat attacgctgg
      301 agtgcgtcag ggcgttcagg atgcggcaaa agattcttca gtacaggtac agttaattga
      361 aactaacgcc cagggggata tttcgaaaga aagtaccttt gttgataccc tcgtggcgcg
      421 taatgtcgat gccattattt tatcggcagt gtctgaaaat ggcagtagcc gtaccgttcg
      481 tcgcgccagt gaagcgggta ttccggtgat ttgctacaac acctgtatta atcaaaaggg
      541 tgtcgataaa tatgtctcgg cgtatctggt cggcgatcca ctggaatttg gtaaaaaact
      601 gggtaacgct gccgccgatt attttattgc caataaaatt gaccagccga aaattgccgt
      661 catcaattgc gaagcctttg aagtttgtgt gcagcgacgt aaaggatttg aagaagtatt
      721 aaaatcccgc gttcccggcg cgcaaattgt cgctaaccaa gaagggactg ttttagataa
      781 agcgatttcc gttggtgaaa aactgattat ctccacgccg gatctcaacg ccattatggg
      841 ggagtcgggc ggtgcgacac tcggcgcggt aaaagcggta cgtaatcaaa atcaggccgg
      901 aaaaattgct gttttcggtt cggatatgac aaccgaaatt gctcaggagc tggaaaacaa
      961 tcaggtgctg aaagcggtag tggatatttc cggtaagaaa atgggcaatg ctgttttcgc
     1021 gcaaacattg aaggttatca ataaacaagc cgacggtgaa aaagtgattc aggtgccaat
     1081 cgatctctat accaaaacgg aagatggtaa acagtggctg gcaacgcacg ttgatggtct
     1141 gccctaatcg ctgattcgca gaggtcccta tgttcacggc aacagaggca gtcccggtag
     1201 caaaagtggt ggcaggaaat aagcgttacc ccggcgtcgt tgcgctggat aacgttaact
     1261 tcacgctcaa taaaggcgaa gttcgtgcgc tgttaggtaa aaacggcgcg ggcaaatcga
     1321 ctctcattcg aatgcttacc ggcagcgaac gcccggatag cggtgatatc tggattggcg
     1381 agacgcgact ggaaggtgac gaagctacgt tgactcgccg tgccgctgaa ctgggggttc
     1441 gtgcggttta tcaggaatta agtctggtgg aagggctgac ggtggcagaa aatctctgcc
     1501 ttggtcaatg gcctcgccgc aacggcatga ttgattacct gcaaatggcg caggatgccc
     1561 aacgttgctt acaggcgctg ggcgttgacg ttagtcctga acaacttgtt tcaacgctaa
     1621 gcccggcgca aaagcaactg gtggaaattg cacgggtgat gaagggcgag ccgcgcgtgg
     1681 tcattcttga tgaacccacc agctcgcttg ccagtgcaga agttgaactg gtgatcagtg
     1741 cggtgaaaaa gatgtcggca ctgggcgtgg cggtgattta tgtcagccac cggatggaag
     1801 aaatccgccg cattgcctcc tgtgccaccg ttatgcgcga tggtcaggtg gcgggcgatg
     1861 tgatgctcga aaacacctcc acgcatcata ttgtgtcgct aatgctaggg cgtgatcacg
     1921 ttgatattgc gccggttgcc cctcaggaaa ttgtggatca ggccgtactg gaagtccgtg
     1981 cgttacgcca taagcccaag ctggaggata tcagctttac gctacgtcgt ggcgaagtgc
     2041 ttggcattgc tggcctgctg ggggcagggc gcagtgaatt gctgaaggcg attgttgggc
     2101 tggagacgta tgaacagggc gaaattgtta tcaacggcga gaaaatcatg cgccccgatt
     2161 acggcgacat gctgaaacgc ggcattggct atacgccaga aaaccgcaaa gaagcgggga
     2221 tcattccctg gctgggcgtt gacgaaaata cagtgctgac caatcggcaa aaaatcagca
     2281 ccaacggtgt gctgcaatgg tccaccatcc gccgcctgac cgaagaggtg atgcagcgga
     2341 tgacggtcaa ggctgccagt agcgaaacac ccatcggcac gctttctggt ggcaatcagc
     2401 aaaaagtggt gatcggtcgt tgggtctatg ccgccagcca gattttgttg ctcgacgagc
     2461 caacgcgcgg cgtcgatatc gaagccaaac agcagattta ccgtattgtc cgtgagctgg
     2521 ctgccgaagg aaaaagcgtg gtgtttatct ccagtgaagt ggaggaattg ccgctggtgt
     2581 gtgaccgcat cctgttatta cagcacggca cgttctcgca ggagtttcac tcgccggtca
     2641 atgtggatga gctgatgtcc gccattctgt ctgtgcactg atgaatttta ccgaggaacc
     2701 tgaagatgtc tgcttcgtca ttaccattgc cgcagggcaa gagcgtctcg ctcaaacaat
     2761 ttgtcagtcg ccatattaat gagatcggtt tgctggtggt gattgccatt ctttatctgg
     2821 tcttctccct gaacgcgcct ggctttatct cattgaataa ccagatgaac gtgctgcgcg
     2881 atgccgccac cattgggatt gccgcctggg cgatgacgct gattattatc tccggtgaaa
     2941 ttgatgtcag cgttgggccg atggtggctt ttgtctcggt gtgcctggca tttttgctgc
     3001 aatttgaagt tccgttggcg attgcttgtc tgctggtgtt gctgttaggc gcgctgatgg
     3061 gaacgctcgc cggggtgctg cgcggcgtgt ttaacgtacc aagtttcgtt gccacactgg
     3121 ggctgtggag cgctctgcgc ggaatggggc tgtttatgac caacgcacag ccagtgccga
     3181 ttgacgaaaa cgaggtgctg gactggctgg gcggacaatt tctcggtgtg ccagtatccg
     3241 cgctgatcat gatggtgttg tttgcgctgt ttgtgttcat tagccgaaaa accgccttcg
     3301 ggcgctcggt ttttgccgtt ggcggtaatg ccacggcggc gcagttgtgc ggtattaacg
     3361 ttcgtcgagt gcgcattctt atctttaccc tttcgggatt attagcggcg gtgaccggca
     3421 ttttgttggc ggcacgcctc ggttccggta acgcaggtgc cgcaaacggt ctggagtttg
     3481 acgtcatcgc cgcggtcgtg gtcggcggta cggcactttc cggcggtcgc ggttccctgt
     3541 tcggtacatt gcttggtgtg ctggtgatta cgctaatcgg taacggtctg gtgctgctcg
     3601 gtattaactc ctttttccag caggtggtac gcggcgtcat catcgtggtg gcggtgctgg
     3661 cgaatatctt gctgacccag cgaagcagta aagcgaaacg ctaaacctta acgcccctgg
     3721 cgcgggtgcg gcagggatga aaattaatcg tattgttaat gagggattta tgaaaacgat
     3781 gctggcagct tatttaccag gaaattcaac cgtcgatctg cgggaagttg cggtgccgac
     3841 gccggggatt aaccaggtac tgatcaaaat gaaatcctcc gggatttgcg gaagcgatgt
     3901 ccactatatc tatcatcaac accgtgccac ggcggcggca cccgataaac cgttatacca
     3961 gggatttatc aacggtcatg aaccgtgcgg gcagattgtg gcgatggggc aaggctaccg
     4021 ccattttaaa gagggcgacc gcgtgctggt gtatcacatt tctggctgtg gtttttgccc
     4081 gaactgccgc cgcggctttc ctatctcttg tactggcaaa ggaaaagcgg cttacggctg
     4141 gcagcgtgac ggcggtcatg ccgaatatct gctggcggaa gaaaaagatc tgatcctcct
     4201 gccggatgcg ctgagctacg aagatggtgc gtttatcagt tgcggcgttg gcacggccta
     4261 tgaagggatt ttgcgcggcg aagtttccgg cagcgataac gtgctggtgg tcggtctggg
     4321 ggcggtcggc atgatggcga tgatgctggc gaaaggtcgc ggtgcaaaac ggatcatcgg
     4381 cgttgatatg ctgccggaac gtctggcgat ggcaaaacag ttagggggga tggatcacgg
     4441 ctatttagca accaccgaag gtctgccgca gataattgct gaacttaccc acggtggcgc
     4501 ggatgttgcg ctcgattgtt ccggtaatgc cgcaggtcgc ttgctggcac tgcaatccac
     4561 tgctgactgg gggcgggtgg tttacattgg tgaaaccgga aaagtggaat tcgaggtcag
     4621 cgctgatctg atgcaccatc aacggcggat tatcggctcc tgggtgacca gtctgttcca
     4681 tatggaaaaa tgcgcccacg atttgactga ctggaagctg tggccgcgta acgccattac
     4741 ccatcgcttc tcgctagaac aggcaggtga tgcctatgtg ctgatggcga gcggcaaatg
     4801 cgggaaagtt gtgattaact tcccggatta aggagttagc ggatgaccat ctatacctta
     4861 tcccacgggt cgctaaagct ggacgtttct gaccagggcg gcgtaatcga aggcttctgg
     4921 cgcgatacga cgccgctctt acgtcctggt aaaaaaagcg gtgtggcaac cgatgcctca
     4981 tgttttccgc tggtgccatt tgcaaaccgg gtgagcggca accgttttgt ctggcagggg
     5041 cgtgagtatc aactgcaacc gaatgttgag tgggatgcgc actatctaca cggcgatggc
     5101 tggctgggcg agtggcaatg tgtctcgcac agcgatgata gtttgtgtct ggtgtatgaa
     5161 catcgcagcg gtgtctatca ctatcgggta agtcaggcgt ttcatttaac ggcggatacg
     5221 ctgacggtga cgctctctgt caccaatcaa ggggcagaga cgctgccatt tggtaccggc
     5281 tggcatcctt attttccgtt gtcaccacaa acgcggattc aggcgcaggc gagcggttac
     5341 tggctggaac gggagcagtg gctggcgggt gagttttgcg agcagctacc gcaggaactg
     5401 gattttaacc agctcgcgcc gttgccgcgt cagtgggtga acaatggttt tgccggatgg
     5461 aatggtcagg cccgcatcga gcagccgcag gaaggttatg ccatcatcat ggaaacgacg
     5521 ccacctgcac cgtgttattt catctttgtt tccgaccctg cgtttgataa aggatatgcg
     5581 tttgatttct tctgtctgga accgatgagc catgcgccgg atgatcatca tcgcccggaa
     5641 gggggtgatc tcattgcgct agcaccaggg gaatcaacaa cctcagagat gtcgttgcgg
     5701 gtagagtggc tgtaatttat aaaatgccgg ataaggcgtt cacgccgcat ccggcatctt
     5761 tcctaacgcc gatcgagaga aattgccccc ggtccggtaa ttgccaatag caagaacgcg
     5821 ccagcgatac tgacattctt ccagaaatta atcatatttg gcccaaccgc atcgccggtc
     5881 atatcccagt aatggtgacc aatcaccgcc gtacccagcg tgtagaaaat aaacagcacc
     5941 gccagcggac gggtgaaaaa gccaagcacg attaatatcg cggcgggcac ttccataact
     6001 accgcaataa tcgctgccag catcggcatt ggcgcgccca acgaggccat atattggacc
     6061 gtaccgtcaa agcccatcat tttgggaaaa ccaaaaataa tgaaaattaa gaccacggcg
     6121 atacgggcaa ttaataacaa aacggggcgg gcagctccaa aatcaaaata acgtagtgtg
     6181 ttcataacca atccctttgc aagataatgt gatttaaacg taatacacac tcttgcacct
     6241 cgccacgccc caggcattga gatattcgct aattatcctg gttacagtga tttaagcgcg
     6301 atgttctcat ctatcagcag tttcgcatca aacgttttgc cgctctggat ccatttgcga
     6361 attgggcgaa tctcacgccc ctgattcagc gtgaccgcac cgataagcac gccgttttgc
     6421 agattaaacc aaatcgcctt ctgagtttct gggttgccac gacaaagcta gtcatcgcca
     6481 cgcatatcgc caataaactg taagttatca ctgtactgat cgctccagaa ccacggcggc
     6541 ggcagtagcg gtagcggtag ccctaacatt gcggcagcgg caatttgcgc ctggttattg
     6601 gcgttttccc agctttcgca gcggtgtagt gcaccattat caagacgagt gattgccaca
     6661 tcgccaccgg caaagatcgc gggatcgcag gtgcggcaag cctcatcaat gacaatgcca
     6721 ttggcagtat caaggttggc ctcgcgagcc agttgctcgt tggcgctgat actaataccg
     6781 taaatcacca catcagcctg aagcgtttcc ccactttgca gcgtcagttc tactttttca
     6841 ccatcgacca catgttcaat ggcattattg agcagaatgc gcacaccagc ctgctgatgg
     6901 cgctgtaaaa gatagcgttg cacgggcggt ggtgcattac ggcccatgac ggttgccgcc
     6961 agttcaatca ctgtcacctt acatctgcgc tgcgtggcgc tggcagccag ttccagacca
     7021 atagttccgg caccgacaat cacgactgac cgttcgggct gcagaacttc tcgcagtctg
     7081 gcggcatcac cggcatggtg tagggtaaag cagcgttctc ccagtgcatc aagcaacggc
     7141 agcggtcgag ctgccgcgcc ggttgctata aaaagctgat cccagtgcca gctttcgccg
     7201 ttggttaaca ctaactctcg tgtgtcgcga cccagcgttt tgatggttac accggaatgc
     7261 agatgaacat tgttttcctg ccaccagtta gcgggtaaca cctgctgtaa ctgcggggaa
     7321 tcttccagca acatggattt cgagagtgga ggtcgttcat aaggaagatg tcgctcatcg
     7381 gaaaacagat gcagctcacc ggtgaaccct tgctggcgta gcgaggccgc agccattgcc
     7441 gccgcttgcc cgccaccgac aataatgatc gttttttctt tcatcgtgct tccttaaaga
     7501 tccagcccag ccgctacgtg gcgaatgccg cgaatcgcta aacccgcatc agcgttgatc
     7561 atcacaccgc ttaatgcgcg attattgcgc cgcgatgcca acatcacata cggccccgta
     7621 aaatccgccg gttgcgggaa aaattgcagc ggtaaaatgg cggcaatttt ctccggcgtc
     7681 agagactgca ttatcgaggt ttcactttgc ccgagcgcct gtgggccgcg caggtcgctg
     7741 gccataccac acgggccgac gccattcacc cgcactttcg gtgccagttc ataagccagt
     7801 tggcgaataa gtccggttgc ggcatgttta ctggtggtgt acagcgggcc accgccgcca
     7861 ggataccagg cggcatttga cagtgtgaaa atcatgctgc cttcactggc gattaacgcc
     7921 ggagcgcagg cttttgcgcc cagcaggtaa ccgagtacgt tgacgttaaa cagctcgtgg
     7981 aagccggttt cgagcgtctc tgcgggagta ttaaccagtg aggcattgtg atcccagatg
     8041 cctgcattgc cgataaaaca atccagcttg ccggaacgag tcaggatctg atcgaccgcg
     8101 cgttgataat cggcataaca ggtcacgtta ccttccaccg ccagaatatg ttcgccaaat
     8161 cgctgacgca gactggcgac ttttgccgcc gacagttcca gcgcggcaac ctgcgcgcct
     8221 tcttcgataa atcgctcgac cagcgccagc cctaatcccg atccgccgcc ggtaataaaa
     8281 atggactcgt tatgcagatc gctcatggct gcgcctccgg taagtcgatg aaaatgtcac
     8341 caccttcaac gtgtactgga taagtggtga gcggatcggt ggcgggcagg cataacgctt
     8401 tccccgtttt caggcaaaaa ctggcggcgt gtagcgggca ctccaccgtg gcgtcatctt
     8461 ccagataccc ttctgacatc gacgcattac catggctgca acgatcgtta atggcataaa
     8521 actcgccgcc cacgttgaac aaggcgatga cgggcgaggt atctatccgg agagcctcac
     8581 cctccggcac atccgcaacg ggacacgcat aaattcgatt catcagaaca gtacactcag
     8641 gttatgggaa gtgatcaccg cttgatccag gacgatatcc cgttccagca agcgccagtt
     8701 gtcatcttcc agacggcgaa ctttgtcgaa acgcgttcca acatagaatg tttcatcgcg
     8761 ctctttttgt gcccgataaa gtagataatt caccctcacg gcaaatacgt ttgggatgtc
     8821 ggtttcgctt acctggcagt tgctgattaa gtgacgagtg cgtgacggcg gctcttctgc
     8881 ccaggccatg cccgtttcca gacgggcgat tcgccgctcc agctggtctt tggtgtcatt
     8941 gaaaatccag gtcgtcggcg gctggacgcc tttgcgacgg tcgcgtgttt gcgcgttaac
     9001 cgtggtgcgc atggtgtaac gaatctcttc gtcgagctgt gccaaccagt cacgaaattt
     9061 ccagtcatcc agcagactgg cttcgtgaaa gagaaactgg ctaatgcggt gatgtaactc
     9121 cagtgaaact tgcgcactca tttcatcacc tcctgctggt aagcggcggt tttatcgagc
     9181 acttcctgcc agctttcgct actcagaaga tcggcccagc gttggtacat tccacgagcg
     9241 gcagtttctg aaaagatata gttagtaatg ccaggaatgc cgtcgtcgcg gcgcttttcc
     9301 tgaccaagcc ccatttccag acacagtttg ctgttgcggg cgcggtggcc tttaagcaat
     9361 ttctggattt cacaccagtt ctccgagtca tcctgctcga gaaaaccagc aggaccaaaa
     9421 gcacgagtgg cgctgttttc aaaagcggct ttaacttcat cggaggcggc tttgtcagta
     9481 atacagaacg cccacacttc aacttgatca gggccgcgcg gatgccagac gcggagtgtg
     9541 gcagtgccgt ttagccatga aagcgtgggg aatatattgt tatgacccgc caggcgcagg
     9601 gcgcgaactt cacctaaacg ttgttctgct tcggcatagg tttcgcggta atagcttgaa
     9661 actgcgccat cgacccagac attagcatcc ggtttttcag taaagaagaa accgctaccg
     9721 tgaccgtcct gaccaaattg cagcgcatct ttggcggttt cccacaccgg gcgggcggtt
     9781 tgtccatcac cgaggcgctt atcgctgcca tcatctttcg cccctaatac ctgaacggca
     9841 gaagcatggc tgaacagagc atgatactgg tcactggcga actgctctgc cgggaatttc
     9901 cagttacagt tgatcaccca cttttgtacg ccgccgacaa tttcggtgcc gccttcgcga
     9961 cgatccagca tgccatccag ataccaggca atgtcaccca ggtaatcatg caggcccggt
    10021 gcgctggtat cccagttgcc aaaaatcagt cctttataac tctccacaca aggaacttcg
    10081 tttagccccc agtgggattt acacaaccct tgtgggtagg cgcgaggttc cagcggtaca
    10141 tcgatcaact cgccgttaat gccataagac cagccgtgat acgggcaggt aaaggcgcga
    10201 gtgttgccac aatctgcata actcacacgc atggcccggt ggcggcattg gttaagaaac
    10261 gccttgatgc tgccgtcttt ctggcgcacc acgacaactg catcttctcc catgtaggtg
    10321 ttaaagaaat caccgggttt tgggatctgg ctttcgtggg cgagaaatag ccagcaacga
    10381 ccgaaaatac gctcaagctc cagttggtaa atgtccgggt cggtataaat acgcggagtg
    10441 acccgaccgt tttgggtgtc aatcagttgg taaatgttca aatctgaggg tgtggtcata
    10501 ttttttaatc ctgaaagata agatgtattg accttctatt tttgtgccat aaggatgttg
    10561 ctggttggtt gctaatatgt gaaatagtta atttctacca gctgagacgt tttttcgata
    10621 acaaggggaa ggtgatggaa ctacggcatt tacgctattt cgtcgcagtg gcgcaggcac
    10681 tgaactttac ccgtgcggcg gaaaaactgc atacctcaca gccttcgtta agcagccaga
    10741 tccgcgatct tgaaaactgt gtcggtgttc cgttactggt gagggataag cgcaaagtcg
    10801 cgttgacggc ggcgggagaa tgttttctcc aggacgcgct ggcaatcctc gaacaagcgg
    10861 aaaatgccaa attacgggcg cggaaaattg ttcaggaaga cagacaatta accattggct
    10921 ttgtgccatc ggcggaagtg aatttattgc caaaagtatt accgatgttt cgtctcagac
    10981 agccagacac cttgattgag ctggtgagtt taatcaccac gcaacaggag gaaaaaattc
    11041 gccgtggtga actcgacgtc ggcttaatgc gccatcctgt ttatagcccg gagattgatt
    11101 atctggagct ttttgacgaa ccattagtgg tggtgttacc ggttgatcac cctttagcac
    11161 atgaaaaaga gatcaccgcc gcgcaactgg atggcgtgaa tttcgtcagt accgatccgg
    11221 cgtattccgg ttcgcttgcg ccgatcgtta aagcgtggtt tgcgcaagaa aacagccagc
    11281 caaatatcgt ccaggtggca acgaatattc tggtgaccat gaatctggtg ggcatggggc
    11341 tgggcgtcac tttgataccc ggttatatga ataattttaa taccgggcag gttgtttttc
    11401 gtcccattgc cggaaatgtg ccttccattg ctttactgat ggcgtggaag aaaggggaga
    11461 tgaagccggc attacgcgat ttcatcgcca ttgtgcagga acgtttggca agcgtaacgg
    11521 cataattacg cattattttt gcaacatgcg ccgcggatcg cagtttttta gtgcttgtga
    11581 actggcgtta cagacatcgg gaagcaggct cgaatattct tatttttcaa cttgcaccaa
    11641 cattcgatta accccagact aacgcctcct gacgggaggg actcatggtt ttgcaatcca
    11701 cgcgctggtt ggcgctcggc tatttcacat acttttttag ttacggcatt tttttacctt
    11761 tctggagcgt ctggcttaaa gggattggtt taacgccaga aaccatcggc ctgttattgg
    11821 gggcaggtct ggttgcccgt tttctcggga gtttgctcat cgcgccccgc gtcagcgatc
    11881 cttcccgcct gatttccgcc ttgcgcgtgc tggcactgct gccacttctc tttgctgtcg
    11941 ccttctgggc gggggcgcac gtagcgtggc tgatgctggt gatgattggc tttaacctct
    12001 ttttctcacc gctggtaccg ttgaccgatg cactggcgaa tacgtggcaa aagcagttcc
    12061 cgcttgatta cggcaaagtg cgactgtggg gctcggtggc gtttgtcatt ggctcggcgc
    12121 tgacgggcaa actggtcact atgtttgatt atcgggtgat cctcgtgctg ttgacgttgg
    12181 gcgtggcatc catgctgctc ggctttctca tccgtccgac gattcagcca caaggggcaa
    12241 gccgccagca ggagagcacc ggttggtctg cgtggttggc gctggttcgc cagaactggc
    12301 gctttctggc ctgcgtttgt ttattgcagg gggcacatgc ggcctattac ggttttagcg
    12361 ccatttactg gcaggcagct ggctactcgg cctcggcggt ggggtatttg tggtcgctgg
    12421 gcgtggtggc ggaagtcatt atctttgcgc tgagtaataa acttttccgc cgttgtagtg
    12481 ctcgcgatat gctgttgatc tcggcgattt gcggcgtagt gcgctggggc attatgggag
    12541 caactacggc gttgccgtgg ttgatagtgg tgcaaattct gcattgcggc accttcacgg
    12601 tctgccacct ggccgccatg cgttatattg ctgctcgcca ggttagcgaa gtcatccgtt
    12661 tacaggcggt ttactctgcc gtcgcgatgg gcggcagtat cgctatcatg accgttttcg
    12721 ccggtttcct gtatcaatat ctgggccacg gtgtgttctg ggtaatggcg ctggtggcgc
    12781 ttccggcaat gtttttgcgc ccgaaagttg ttccctcatg ctgattcgag cattttgcgg
    12841 atctgctcct gttgaggtgt tgtcagcgtc aggtccgtat agatcagcgg tggtgaaaag
    12901 agcggtaacg gcatggtata aggcgcaata atcagtgccg cgccacgcgg agcgcctgtc
    12961 tgcaaaaatg cctttaccga catatgctta atgttgagcg gcagtaacgt tagttcgcgt
    13021 aactgctgct caatctgcgc ttctcgctcg ctatcattcc cggtcagtag aataatctgt
    13081 ttttcatgca gatcgttttc ctgcattagc caggcaccga aaatcaccgc gaccagacca
    13141 ctttcctcat cagaaagatg gacgccgtat tcactttcaa atccggccag cgccgcgcgg
    13201 gtgatgcgta ccaggcgtgg atacagtctg gcgaactctt ccggcagggt attatcaata
    13261 ccgatggcaa acaaactgcg atttaacgcc tgggcgaggt ggatataaag ctgatcacat
    13321 aacccctgtt catcataaaa acgaacattt cccagctcac gaaaatggtt taccagacgt
    13381 ttgatagact gacgcaattg tctgtcccgc tgatgcgcat cccgcagtgg atcgggaacc
    13441 cgcaacatgg aaaaaagtaa tgccataaac agtggctcat caggcggtac atgctggagc
    13501 gcccgacgct gccagtggcg tccaatttcc tgcgctacct gaaattcaag gcaggattcc
    13561 gcccagcgac gttggagcgg attaaactga ggcgtaattc cagcgtggtg ctgcagcaaa
    13621 caatattgca gatacagaca caggaaatga atatcgcgcg attcaaagcg tttttgcagc
    13681 cgtcgggagc agagattcac taacgcttgt agattggtat cgtcgtaaaa gttacgcgca
    13741 attccgcgcc gcttcagttc actcttcagg gcgggggtga attggctggt aataaatgac
    13801 ggacacagac ggaaaccacg tcgtagccag tgaaataaac acaaacgttg gttaagcact
    13861 gtaccttcaa cccggtagct accgtcataa ccagtcgtca gtgtgagttg atgatagcgc
    13921 aggatctccc gtcctgtttc gctgatatca agactggcaa tatcatcatc cacaccatta
    13981 agctcgctga aggttgccat ggtggcggtc aaccccggct gaaagagcgt cagcaagatc
    14041 tggcagcggc gctggggagc cgaaaggaca gatggtggag caagcgtagg catcatctgt
    14101 agatctccca taagcataat tgataagaat agctaaagtt tgctcaggga aatggcgaag
    14161 ggaatgctaa tcatcagaaa tgttggcaag cgtcacagaa tttttctcat tttaaactgt
    14221 tctgaccagt ggattagcat gtcttaaggt ggttttgtga atgattatcg tgctagcgat
    14281 catgggttag ttctgcggat tcaacgcgct aaggataaag tccaccatct gttcgcgata
    14341 aacattgccg tgacgatcgg aaaaagcatc aatgtgttcg ccatccggga ttaggatcaa
    14401 ccgttttggc tcttttgcca ggctatacaa cttttcgctg tgctgccatg ggataacgtg
    14461 atcggcttta ccgtgaatga gtaaaagcgg gatcgggctg acgctggcga tgtaattttc
    14521 gccgctgtaa ctctcatcaa gtaagtagcc actgccgggg agcatctggt tggcgatggt
    14581 tgcataagag gcaaatgtgg agtcgaggat caccgcacgt atgccttcac gatcaccctg
    14641 accaataaca gcaagaatat tcgccccgcc aatactctgc ccgaacagca ccagccgttg
    14701 tgggtttaca tcactgcgat ggcgcaccac attgatggcg ctttgcgtat cgtccagcaa
    14761 tccggcctgg gacggagtgc cttttgattt accaaacccg cgataatcaa acataaaaac
    14821 gttgaaatta cgctcaggta accaactgac cagcggccag tgggcggaca tatttccggc
    14881 attgccgtga gcatgaatga tggttgcgat ggcgttgtca gcagggcccg tcgaagaagg
    14941 aataaaccag ccttgcagac gagtaccgtc cttagccgta aattcgacgg attccgccga
    15001 ccaggggtcg ggaccgtaaa ttttatcgtc aggataatag aagaagttga tggcaacgcg
    15061 cagcactaaa taaacgcaga aggcaacaac aaagagaata aacagaattt tgggaacgcg
    15121 tttgttcact ggcagtgcca taacctttcc ttgcgacttc tttatcatcc attaatcggg
    15181 gaaagatggt gggagagtga cgaaagaaag taaagcggtg aggaagtatt tcggaatagc
    15241 gcctgaaagg cgagggcggg tgagtgatat cacccgcctg agtcattaac gcttcagagc
    15301 gtcgcttaac tcgtcacgca tgttcgccag tatggctttc acaacacgcg ggttaccagc
    15361 aacgatgtta ccggtcagca tatagttatg accaccggtg aagtcgctga cgatgccgcc
    15421 cgcttcacga accagcagat cgcctgccgc gaaatcccac ggacgcagac cgatttcaaa
    15481 gaaaccgtca acacgacccg cagcgacgta agccagatcc agcgccgcag aaccggtacg
    15541 acggaagtct gcacattcgt tgaacagctt gccgacgatg ttgatgtagg tagtggcgta
    15601 ctgttttgct ttgaacggga agccggtcgc cagaatggta ccgtcgagat cgcgagcggt
    15661 gctaccgcgc agacggtagc cgttcagctg tgcgccctga ccgcgagtgg cggtgaacag
    15721 ttcgttacgc ataggatcgt ataccacagc aacttcggtg cggcctttga tacgaacggc
    15781 gatagatacc gcgaagtgcg gcagacgttt gataaagttg gtagtaccat ccagtggatc
    15841 gataacccat tgaacatcct gatcagtacc ttcaagttca ccgctttctt cggtgatgat
    15901 ggtgtgctgt gggtaagatt tacgaatcgt gtcgataatc accgcttcgg cagctttatc
    15961 tacgttggtc acgaaatcgt tactgccttt ctggctcgct tctacagcgt ccggggtttc
    16021 atagtttttg gcaattaaat tacccgcctt gcgcgctgcg cgcacggcga tgttcagcat
    16081 cggatgcatc agtctctctc actggatgtt aaagaacggg aaaacggcgc agagtatagc
    16141 agcgagatcg gcatctgtct tcgttttatg ataagatgcg tgaataatct ttagatgaag
    16201 aaagacaatg ctgcaaaata ttcgaattgt gctggtggag acgtcacaca ccggcaatat
    16261 gggttctgtt gcccgtgcca tgaaaacaat gggattaacc aatctgtggc tggttaatcc
    16321 actggtgaaa cccgactccc aggcgattgc cctggctgca ggggccagcg atgtgattgg
    16381 taatgctcac atcgtcgata cgctcgacga agcgttagcc ggttgtagcc tggtggtggg
    16441 caccagcgca cgctcccgca cgctgccgtg gccgatgctc gacccgcgcg aatgcggtct
    16501 gaaaagcgtc gctgaagcgg caaatacccc ggtggcgctg gtgtttggtc gtgagcgcgt
    16561 cggcttgacc aatgaagagt tgcagaaatg ccattatcat gtcgcgattg cggcaaaccc
    16621 ggaatacagt tcgctgaacc tggcaatggc ggttcaggtt attgcctatg aagtgcgtat
    16681 ggcctggctg gcaactcagg aaaatggcga gcaggtcgag catgaagaga cgccgtatcc
    16741 gctggtcgat gatctggagc gtttttatgg tcatctggaa caaacgctgc tggcaaccgg
    16801 ttttatccgt gaaaaccatc cggggcaggt gatgaataaa ttgcgccgtc tgtttacccg
    16861 tgcgcgcccg gaaagccagg aattgaatat cctgcgcggg attctggctt ctattgagca
    16921 gcagaataaa ggtaacaagg ccgaataaca gtggttgcct gatgcgacgc ttaacgcgtc
    16981 ttatcaggcc tacaatgtac agaatcgtag gtcggataag gtgttcacac cgcatccgac
    17041 aacaggtaca aacgccacga taaaaaaatg gcactgaagg ttaaataccc gactaaatca
    17101 gtcaagtaaa tagttgacca atttactcgg gaatgtcaga cttgaccctg ctatgcaata
    17161 cccccacttt tacaataaaa aaccccgggc aggggcgagt ttgaggtgaa gtaagacatg
    17221 agactgacat ctaaagggcg ctatgccgtg accgcaatgc ttgacgttgc gctcaactct
    17281 gaagcgggcc cggtaccgtt ggctgatatt tccgaacgtc agggaatttc cctttcttat
    17341 ctggaacaac tgttttcccg tctgcgtaaa aatggtctgg tttccagcgt acgtggacca
    17401 ggcggtggtt atctgttagg caaagatgcc agcagcatcg ccgttggcga agtgattagc
    17461 gccgttgacg aatctgtaga tgccacccgt tgtcagggta aaggcggctg ccagggcggc
    17521 gataaatgcc tgacccacgc gctgtggcgt gatttgagcg accgtctcac cggttttctc
    17581 aacaacatta ctttaggcga actggttaat aaccaggaag tgctggatgt gtctggtcgt
    17641 cagcatactc acgacgcgcc acgcacccgc acacaagacg cgatcgacgt taagttacgc
    17701 gcttaataaa aagaattcag aatcaggccg gagtgctaaa tactccgtaa acacggtcgt
    17761 acatccagcc ggtagcctga ttccttgcat tgagtgatgt acggagttta tagagcaatg
    17821 aaattaccga tttatctcga ctactccgca accacgccgg tggacccgcg tgttgccgag
    17881 aaaatgatgc agttcatgac gatggacgga acctttggta acccggcctc tcgttctcac
    17941 cgtttcggct ggcaggctga agaagcggta gatatcgccc gtaatcagat tgccgatctg
    18001 gtcggcgctg atccgcgtga aatcgtcttt acctctggtg caaccgaatc tgacaacctg
    18061 gcgatcaaag gtgcagccaa cttttatcag aaaaaaggca agcacatcat caccagcaaa
    18121 accgaacaca aagcggtact ggatacttgc cgtcagctgg agcgcgaagg tttcgaagtc
    18181 acctacctgg caccgcagcg taacggtatt atcgacctga aagaactcga agcggcgatg
    18241 cgtgacgaca ccatccttgt ttccatcatg cacgtaaata acgaaatcgg cgtggtgcag
    18301 gatatcgcgg ctatcggcga aatgtgccgt gctcgtggca ttatctatca cgttgatgca
    18361 acccagagcg tgggcaaact gccaatcgac ctgagccagt tgaaagttga cctgatgtct
    18421 ttctccggtc acaaaatcta tggcccgaaa ggtatcggtg cgctgtatgt gcgtcgtaaa
    18481 ccgcgtgtac gcatcgaagc gcaaatgcac ggcggcggtc acgagcgcgg tatgcgttcc
    18541 ggcactctgc ctgttcacca gatcgtcgga atgggcgaag cctatcgcat cgcaaaagaa
    18601 gagatggcga ccgagatgga acgtctgcgc ggcctgcgta accgtctgtg gaacggcatc
    18661 aaagatatcg aagaagttta cctgaacggt gacctggaac acggtgcgcc gaacattctc
    18721 aacgtcagct tcaactacgt tgaaggtgag tcgctgatta tggcgctgaa agacctcgca
    18781 gtttcttcag gttccgcctg tacgtcagca agcctcgaac cgtcctacgt gctgcgcgcg
    18841 ctggggctga acgacgagct ggcacatagc tctatccgtt tctctttagg tcgttttact
    18901 actgaagaag agatcgacta caccatcgag ttagttcgta aatccatcgg tcgtctgcgt
    18961 gacctttctc cgctgtggga aatgtacaag cagggcgtgg atctgaacag catcgaatgg
    19021 gctcatcatt aatcggtatc ggaatcagga gaatttataa tggcttacag cgaaaaagtt
    19081 atcgaccatt acgagaatcc gcgtaacgtg ggttcctttg ataacaacga cgagaacgtc
    19141 ggcagcggca tggtgggtgc accggcctgt ggcgacgtaa tgaagttgca gattaaagtc
    19201 aacgatgaag gtatcattga agacgcgcgt tttaaaactt acggctgcgg ttccgctatc
    19261 gcttccagct ccctggtcac cgaatgggtg aaagggaagt ctctcgacga agcgcaggcg
    19321 atcaaaaaca ccgatattgc tgaagaactt gaactgccgc cggtgaaaat tcactgttct
    19381 attctggcag aagacgcgat caaagccgcc attgcggact ataaaagcaa acgtgaagca
    19441 aaataagagt tgaggtttgg ttatgtcgat tacactgagc gacagtgcag cagcgcgagt
    19501 aaataccttt ctggctaacc gcggcaaagg gtttggcctg cgtctgggcg tgagaacctc
    19561 cggatgttca ggtatggctt atgtactgga atttgttgac gaaccgacgc cggaagacat
    19621 cgtgtttgaa gacaaaggcg tgaaagtcgt ggtcgatggc aaaagcctgc aatttctgga
    19681 cggtacgcag ctggacttcg taaaagaagg cctgaatgaa gggtttaaat tcaccaaccc
    19741 gaacgttaaa gatgagtgtg gttgcggcga aagcttccac gtttgatgcg cataccgata
    19801 accccaccgt ggtcgcctgc gcgtggggtt tgttttacct gattcgccgt tatcgcggcg
    19861 gatcgcagcc ctgagaatgt tatggattac ttcaccctct ttggcttgcc tgcccgttat
    19921 caactcgata cccaggcact gagcctgcgt tttcaggatc tacaacgtca gtatcatcct
    19981 gataaattcg ccagcggaag cctggcggaa caactcgccg ccgtacagca atctgcaacc
    20041 attaaccagg cctggcaaac gctgcgtcat ccgttaatgc gcgcggaata tttgctttct
    20101 ttacacggtt ttgatctcgc cagcgagcag catactgtgc gcgacaccgc gttcctgatg
    20161 gaacagttgg agctgcgcga agagctggac gagatcgaac aggcgaaaga tgaagcgcgg
    20221 ctggaaagct ttatcaaacg tgtgaaaaag atgtttgata cccgccatca gttgatggtt
    20281 gaacagttag acaacgaggc gtgggacgcg gcggcggata cggtgcgtaa gctgcgtttt
    20341 ctcgataaac tgcggagcag tgccgaacaa ctcgaagaaa aactgctcga tttttaattt
    20401 ctggaagcta aacatggcct tattacaaat tagtgaacct ggtttgagtg ctgcgccgca
    20461 tcagcgtcgt ctggcggctg gtattgacct gggcacaacc aactcgctgg tggcgacagt
    20521 gcgcagcggt caggccgaaa cgttagccga ccatgaaggc cgtcacctgc tgccatctgt
    20581 tgttcactat caacagcaag ggcattcggt gggttatgac gcgcgtacta acgcagcgct
    20641 tgacaccgcc aacaccatta gttctgttaa acgcctgatg gggcgctcgc tggctgatat
    20701 ccagcaacgc tatccgcatc tgccttatca attccaggcc agcgaaaacg gcctgccgat
    20761 gattgaaacg gcggcggggc tactgaaccc ggtgcgcgtt tctgcggaca tcctcaaagc
    20821 actggcggcg cgggcaactg aagccctggc aggcgagctg gatggtgtag ttatcaccgt
    20881 tccggcgtac tttgacgatg cccagcgtca gggcaccaaa gacgcggcgc gtctggcggg
    20941 gttgcatgtt ctgcgcttac ttaacgaacc gaccgctgcg gctatcgcct acgggctgga
    21001 ttccggtcag gaaggcgtga tcgctgttta tgacctcggt ggcgggacgt ttgatatttc
    21061 cattctgcgc ttaagtcgcg gcgtgtttga agtgctggca accggcggtg attccgcgct
    21121 cggcggcgat gatttcgacc atctgctggc ggattacatt cgcgagcagg cggacattcc
    21181 tgatcgtagc gataaccgcg ttcagcgtga actgctggat gccgccattg cagccaaaat
    21241 cgcgctgagc gatgcggact ccgtgaccgt taacgttgcg ggctggcagg gcgaaatcag
    21301 ccgtgaacaa ttcaatgaac tgatcgcgcc actggtaaaa cgaaccttac tggcttgtcg
    21361 tcgcgcgctg aaagacgcgg gtgtagaagc tgacgaagtg ctggaagtgg tgatggtggg
    21421 cggttctact cgcgtgccgc tggtgcgtga acgggtaggc gaatttttcg gtcgtccacc
    21481 gctgacttcc atcgacccgg ataaagtcgt cgctattggc gcggcgattc aggcggatat
    21541 tctggtgggt aacaagccag acagcgaaat gctgctgctt gatgtgatcc cactgtcgct
    21601 gggcctcgaa acgatgggcg gcctggtgga gaaagtgatt ccgcgtaata ccactattcc
    21661 ggtggcccgc gctcaggatt tcaccacctt taaagatggt cagacggcga tgtctatcca
    21721 tgtaatgcag ggtgagcgcg aactggtgca ggactgccgc tcactggcgc gttttgcgct
    21781 gcgtggtatt ccggcgctac cggctggcgg tgcgcatatt cgcgtgacgt tccaggtcga
    21841 tgccgacggt cttttgagcg tgacggcgat ggagaaatcc accggcgttg aggcgtctat
    21901 tcaggtcaaa ccgtcttacg gtctgaccga tagcgaaatc gcttcgatga tcaaagactc
    21961 aatgagctat gccgagcagg acgtaaaagc ccgtatgctg gcagaacaaa aagtagaagc
    22021 ggcgcgtgtg ctggaaagtc tgcacggcgc gctggctgct gatgccgcgc tgttaagcgc
    22081 cgcagagcgt caggtcattg acaatgctgc cgctcacctg agtgaagtgg cgcagggcga
    22141 tgatgttgac gccatcgaac aagcgattaa aaacgtagac aaacaaaccc aggatttcgc
    22201 cgctcgccgc atggaccagt cggttcgtcg tgcgctgaaa ggccattccg tggacgaggt
    22261 ttaatatgcc aaagattgtt attttgcctc atcaggacct ctgccctgat ggcgctgttc
    22321 tggaagctaa tagtggtgaa accattctcg acgcagcgct gcgtaacggt atcgagattg
    22381 aacacgcctg tgaaaaatcc tgtgcttgca ccacctgcca ctgcatcgtt cgtgaaggtt
    22441 ttgactcact gccggaaagc tcagagcagg aagacgacat gctggacaaa gcctggggac
    22501 tggagccgga aagccgttta agctgccagg cgcgcgtcac cgacgaagat ttagtggtcg
    22561 aaatcccgcg ttacactatc aaccatgcgc gtgagcatta acagaggtta gtatgggact
    22621 taagtggacc gatagccgcg aaattggcga agcactgtac gatgcgtatc ccgatcttga
    22681 tccgaaaacg gttcgattca ccgatatgca tcagtggatt tgcgatctgg aagatttcga
    22741 cgacgacccg caggcatcca acgagaaaat cctcgaagcg attttgttag tctggctgga
    22801 cgaagccgag tgaatgaacg ggctgcctgg gggtagcccg ttttgctgtt aaacgaataa
    22861 ggataactaa aatgacagaa gcgatgaaga ttaccctctc tacccaacct gccgacgcgc
    22921 gctggggaga aaaagcaact tacagcatta ataatgacgg cattaccctg catttgaacg
    22981 gggcagacga tctggggctg atccagcgtg cggcccgcaa gattgacggt ctgggcatca
    23041 agcatgttca gttaagcggt gaaggctggg atgcggatcg ctgctgggca ttctggcaag
    23101 gttataaagg cccgaaaggc acgcgtaaag tggagtggcc ggatctggac gatgcccagc
    23161 gccaggaact ggataaccgc ctgatgatca tcgactgggt gcgtgacacc atcaacgcac
    23221 cggcagaaga attgggacca tcgcaactgg cacagcgtgc tgttgatctg atcagcaacg
    23281 tcgcgggcga tcgtgtgact tatcggatca ccaaaggcga agatctgcgt gagcaaggtt
    23341 atatggggct gcacactgtc gggcgcggtt cagaacgttc tccggtattg ctggcgctgg
    23401 attacaaccc aactggcgat aaagaagcac cggtgtacgc gtgcctggta ggtaaaggta
    23461 tcacttttga ctccggcggc tacagcatca aacagactgc gtttatggac tcgatgaagt
    23521 cggacatggg cggcgcggca acggttaccg gggcgctggc atttgccatt acgcgcggac
    23581 tgaacaagcg cgtgaagctg ttcctctgct gtgcggataa cctgattagc ggcaatgcgt
    23641 tcaagctggg cgatatcatc acttatcgca acggtaaaag agttgaagtg atgaacactg
    23701 atgccgaagg gcgtctggtg cttgccgatg gtctgattga tgccagtgcg cagaaaccgg
    23761 aaatgatcat tgatgcggcg accctcaccg gggcggcgaa aactgcgctg ggtaatgatt
    23821 atcacgcgct gttcagtttt gacgatgcgc ttgccggtcg tttactggcg agtgcctcac
    23881 aagagaacga accattctgg cgtctgccgc tggcggaatt ccaccgcagc cagctgccgt
    23941 ctaactttgc cgaactgaac aataccggaa gcgcggcgta tccggcaggc gcgagcacgg
    24001 cggcgggctt cctgtcgcac tttgttgaga actatcagca aggctggctg catatcgact
    24061 gctcggcgac ttaccgtaaa gcgccggttg aacagtggtc tgcgggcgct acgggacttg
    24121 gtgtgcgcac gatagctaat ctgttaacgg cgtaaactaa tatgccggat gcggcgtaaa
    24181 cgccttatcc ggcctacaaa ttctgcaaat tcaacaaatt gcgaatccct tgtaggcctg
    24241 ataagcgtag cgcatcaggc atttgcgccc cgaacgttga atgaattgtg gttcttatgt
    24301 ccgaaacaaa aaacgaactt gaagacctgc tggaaaaagc agcaaccgaa ccggcgcacc
    24361 gcccggcctt tttccgtact ctactggaat ccaccgtctg ggtgcctggt acggcggcgc
    24421 agggcgaggc tgtggttgaa gattgtgcgc ttgatttaca gcactgggaa aaagaagacg
    24481 gcaccagcgt cattcctttt ttcacctcgt tagaagcgct tcaacaggcg gttgaagacg
    24541 aacaggcatt tgtcgtaatg cccgttcgca cattgtttga gatgaccctt ggcgaaacgc
    24601 tcttccttaa tgccaaactg ccgaccggta aagaatttat gccgcgtgaa atcagtttgt
    24661 tgattggtga agagggaaat ccgctgagca gccaggaaat cctggaaggc ggtgaatcgc
    24721 tgatattatc ggaagtcgca gagccgccag cacaaatgat tgattcactc accaccttat
    24781 ttaaaaccat taagccggtg aagcgtgctt ttatttgttc aattaaagag aacgaagagg
    24841 cacagcctaa tttacttatt ggcattgaag ccgatggtga tatcgaagaa attattcagg
    24901 cgacgggaag tgtagcgacc gatacattac ctggcgatga accaatcgat atttgtcagg
    24961 tgaaaaaagg ggaaaaagga attagccact ttattaccga acatattgcg ccattctatg
    25021 aacgtcgctg gggtggtttt ttgcgtgact ttaaacaaaa ccggataatc taaaatatgc
    25081 cgccctaaag ggcggcattc gggcttactt gctgctctga aggttgttaa ccaccagtaa
    25141 attgtcgccg gagagactcc ggttattatt tacttgggaa aacagcgcac cagaaacaat
    25201 aagtacgata cagaagcacc gtatcttcat tgtgctgtcg tgatgcgaag accgcatcac
    25261 gacagcacca ctggcgatat tgccgcgata gcaagcgctg agacgcgctt gctctaacgg
    25321 tcgcggaaca tcctttcctt aagccagcgc ccgcttgcga gacggcggaa agggccgcag
    25381 tggataatac actaacagaa caataataac tctcttttgc ttgacaaaaa gagagttact
    25441 ggcgagtatt gtctccgctg agacgcgctt gcattactac ggtaatacaa cctgctggaa
    25501 aggccgcgaa ccagaccagc aataaaaaaa cgccaaattc ggcggttttt tattgcctgt
    25561 aaaatactta tttcactggc tcaaccggta aatctgcccg cgcgccccat tcactccatg
    25621 cgccgtcgta cagtttcacg tttggcacat ccagcgtcgc gagtgctaac aaaaccacgg
    25681 ctgccgttac accagagccg cagctgacga taatcggttt gtcatagctg acgccgcgac
    25741 caaaaaatat cgcatccagt tcatcggtcg tttttagttc gccttcgcgc accagttccg
    25801 tccacggaac gttcaatgct ccgggaatat gtccgcgacg taaacctggg cgaggttcat
    25861 caacttctgc gttaaaacgt gcagccgggc gggcatcaat aatttgcgcc gtattttcat
    25921 ggcttgccaa taacacatcg gttactttca ccactgcttc aggattaaac gcggcgttaa
    25981 actctccttc cggcagctct actgcacctt cttctaatag caggtcatcg cgctgccagc
    26041 ctgcaag
//
LOCUS       DAJVQH010000046        25990 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJVQH010000046 DAJVQH010000000
VERSION     DAJVQH010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.046"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..108)
                     /locus_tag="OQM87_001986"
     CDS             complement(<1..108)
                     /locus_tag="OQM87_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HCS1508135.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVH"
     gene            complement(178..1167)
                     /gene="araF"
                     /locus_tag="OQM87_001987"
     CDS             complement(178..1167)
                     /gene="araF"
                     /locus_tag="OQM87_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCS1508136.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1964..2467
                     /locus_tag="OQM87_001988"
     CDS             1964..2467
                     /locus_tag="OQM87_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCS1508137.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2547..2798)
                     /locus_tag="OQM87_001989"
     CDS             complement(2547..2798)
                     /locus_tag="OQM87_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCS1508138.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2913..2999)
                     /gene="azuC"
                     /locus_tag="OQM87_001990"
     CDS             complement(2913..2999)
                     /gene="azuC"
                     /locus_tag="OQM87_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCS1508139.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3262..3585
                     /locus_tag="OQM87_001991"
     CDS             3262..3585
                     /locus_tag="OQM87_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCS1508140.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLH
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3756..4253
                     /gene="ftnA"
                     /locus_tag="OQM87_001992"
     CDS             3756..4253
                     /gene="ftnA"
                     /locus_tag="OQM87_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCS1508141.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4290..4529)
                     /locus_tag="OQM87_001993"
     CDS             complement(4290..4529)
                     /locus_tag="OQM87_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCS1508142.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4719..5930
                     /gene="tyrP"
                     /locus_tag="OQM87_001994"
     CDS             4719..5930
                     /gene="tyrP"
                     /locus_tag="OQM87_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCS1508143.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSMPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5992..6657)
                     /gene="yecA"
                     /locus_tag="OQM87_001995"
     CDS             complement(5992..6657)
                     /gene="yecA"
                     /locus_tag="OQM87_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCS1508144.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6853..6939)
                     /locus_tag="OQM87_001996"
     tRNA            complement(6853..6939)
                     /locus_tag="OQM87_001996"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6903..6905),aa:Leu,seq:taa)
     gene            complement(6952..7025)
                     /locus_tag="OQM87_001997"
     tRNA            complement(6952..7025)
                     /locus_tag="OQM87_001997"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6991..6993),aa:Cys,seq:gca)
     gene            complement(7307..7855)
                     /gene="pgsA"
                     /locus_tag="OQM87_001998"
     CDS             complement(7307..7855)
                     /gene="pgsA"
                     /locus_tag="OQM87_001998"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCS1508145.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7912..9744)
                     /gene="uvrC"
                     /locus_tag="OQM87_001999"
     CDS             complement(7912..9744)
                     /gene="uvrC"
                     /locus_tag="OQM87_001999"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCS1508146.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9741..10397)
                     /gene="uvrY"
                     /locus_tag="OQM87_002000"
     CDS             complement(9741..10397)
                     /gene="uvrY"
                     /locus_tag="OQM87_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCS1508147.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10693..10869
                     /locus_tag="OQM87_002001"
     CDS             10693..10869
                     /locus_tag="OQM87_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508148.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10856..11080
                     /locus_tag="OQM87_002002"
     CDS             10856..11080
                     /locus_tag="OQM87_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCS1508149.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(11113..11733)
                     /gene="sdiA"
                     /locus_tag="OQM87_002003"
                     /pseudo
     CDS             complement(11113..11733)
                     /gene="sdiA"
                     /locus_tag="OQM87_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11963..12715)
                     /gene="yecC"
                     /locus_tag="OQM87_002004"
     CDS             complement(11963..12715)
                     /gene="yecC"
                     /locus_tag="OQM87_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCS1508150.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(12712..13380)
                     /gene="tcyL"
                     /locus_tag="OQM87_002005"
     CDS             complement(12712..13380)
                     /gene="tcyL"
                     /locus_tag="OQM87_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCS1508151.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13395..14381)
                     /gene="dcyD"
                     /locus_tag="OQM87_002006"
     CDS             complement(13395..14381)
                     /gene="dcyD"
                     /locus_tag="OQM87_002006"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCS1508152.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14486..15286)
                     /gene="tcyJ"
                     /locus_tag="OQM87_002007"
     CDS             complement(14486..15286)
                     /gene="tcyJ"
                     /locus_tag="OQM87_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1508153.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15374..15925)
                     /gene="fliZ"
                     /locus_tag="OQM87_002008"
     CDS             complement(15374..15925)
                     /gene="fliZ"
                     /locus_tag="OQM87_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCS1508154.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15971..16690)
                     /gene="fliA"
                     /locus_tag="OQM87_002009"
     CDS             complement(15971..16690)
                     /gene="fliA"
                     /locus_tag="OQM87_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCS1508155.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVCHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16855..18507)
                     /gene="fliC"
                     /locus_tag="OQM87_002010"
     CDS             complement(16855..18507)
                     /gene="fliC"
                     /locus_tag="OQM87_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCS1508156.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTVGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18773..20179
                     /gene="fliD"
                     /locus_tag="OQM87_002011"
     CDS             18773..20179
                     /gene="fliD"
                     /locus_tag="OQM87_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCS1508157.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            20204..20614
                     /gene="fliS"
                     /locus_tag="OQM87_002012"
     CDS             20204..20614
                     /gene="fliS"
                     /locus_tag="OQM87_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCS1508158.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20614..20979
                     /gene="fliT"
                     /locus_tag="OQM87_002013"
     CDS             20614..20979
                     /gene="fliT"
                     /locus_tag="OQM87_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCS1508159.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            21057..22544
                     /gene="amyA"
                     /locus_tag="OQM87_002014"
     CDS             21057..22544
                     /gene="amyA"
                     /locus_tag="OQM87_002014"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1508160.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDCIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22578..22991)
                     /locus_tag="OQM87_002015"
     CDS             complement(22578..22991)
                     /locus_tag="OQM87_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1508161.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            23178..24383
                     /gene="yedE"
                     /locus_tag="OQM87_002016"
     CDS             23178..24383
                     /gene="yedE"
                     /locus_tag="OQM87_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCS1508162.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24380..24613
                     /gene="yedF"
                     /locus_tag="OQM87_002017"
     CDS             24380..24613
                     /gene="yedF"
                     /locus_tag="OQM87_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCS1508163.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24720..25390
                     /locus_tag="OQM87_002018"
                     /pseudo
     CDS             24720..25390
                     /locus_tag="OQM87_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(25430..>25990)
                     /locus_tag="OQM87_002019"
     CDS             complement(25430..>25990)
                     /locus_tag="OQM87_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1508164.1"
                     /translation="LARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVT
                     TTVSKNHVMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLE
                     YKQLWRGGPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHA
                     VLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         6437 a         6643 c         6183 g         6727 t
ORIGIN      
        1 atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa cgccgggaaa
       61 cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa cgtctccgtg
      121 aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc cggcaaatta
      181 cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg tgatcagtac
      241 cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc agttgtaaag
      301 catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac cgtagaagcc
      361 ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac caataccgat
      421 gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa gtacggtgct
      481 gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca gcattgagtt
      541 ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat aaatttgttt
      601 ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc ggcgggcggt
      661 atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc agccacgttt
      721 ctgcatctct ttatacagtt cctggccctg acgttcgcca attttagtcg ccgccatcat
      781 caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt catccactgc
      841 aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga gtttcgggtc
      901 cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga tcgcgttcaa
      961 tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat ccttcccggc
     1021 tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct tcaccagaaa
     1081 accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca gaccaatggc
     1141 tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg ttttgtgtag
     1201 ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc ttttatctaa
     1261 aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat aggagagcaa
     1321 tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa gacataagga
     1381 aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata agattagcct
     1441 ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc agtcaatagg
     1501 ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca aagatgtgga
     1561 aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta acagaacacg
     1621 tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc acttcatcat
     1681 gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat taaaattcat
     1741 tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat gatttaagca
     1801 ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa ttgactccac
     1861 gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat ggtgttttga
     1921 tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa ccgctggaat
     1981 gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc tctaccttca
     2041 cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc ttcgcgccca
     2101 ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga gtgtcggcgc
     2161 tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc tggaagaact
     2221 gttccaaaaa acgatggaag aatacgagca acgttcaagc acgctggcgc agttagccga
     2281 tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc tggaaaaaga
     2341 acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca gtgcgaaact
     2401 tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt cacaccagct
     2461 gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag aaaccgctgc
     2521 ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt aaacttgagt
     2581 tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc ggcagcggga
     2641 tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac ttcaacgggc
     2701 gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc taccacatca
     2761 gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg atagcatttt
     2821 gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac cgtcatcagg
     2881 acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc tggcggtacg
     2941 tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg cagtttcatc
     3001 gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata atatgacaac
     3061 catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt aatgttataa
     3121 aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca agaaatagcg
     3181 cctgccaggc atcttttccg gccattgtcg cagcgctgta acgcgtaaaa tagtgctttc
     3241 tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt aataacgctg
     3301 gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc aacaggtatt
     3361 gtacggttgg tttatgacca ggcttttttg catcatgccc ataccgatcg ttatgtcagt
     3421 cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat cccctttggt
     3481 cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac ggaattcact
     3541 ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt ttaatcagcg
     3601 aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat tcgtattttt
     3661 aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt atagtgacgc
     3721 cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa atgattgaaa
     3781 aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag caaatgagcg
     3841 cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt cacgcccagg
     3901 aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc aatttaccgc
     3961 gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa ttattccagg
     4021 aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct catgctgcaa
     4081 tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct gagcagcatg
     4141 aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc aaaagcggcg
     4201 aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac taatgctctc
     4261 cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact ctgatcggtc
     4321 gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc taccagctct
     4381 cccgctgtca tcccttctgc cgagcacgtg tgaaaacgtg cctgttcacc aaaacgcgct
     4441 ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat catcatattt
     4501 aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc tagcgtagcg
     4561 attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt gtacatttat
     4621 atttacacaa tatgtaacgt cgatttgacg aagcagccgt tatgccttaa cctgcgccgc
     4681 agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga accctgggaa
     4741 gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg ccgctggctg
     4801 cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg gcgttgatgt
     4861 gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat accggtctgg
     4921 gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc ttcagtatga
     4981 tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa ttgttggcct
     5041 ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg ctgttgttca
     5101 cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta tttaaccgtt
     5161 ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg ctaccgcata
     5221 ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg tctgcaatcc
     5281 cggtgatttt tacgtcgttt ggttttcacg gtagcatgcc gagtattgtc agctatatgg
     5341 atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc cccctggtgg
     5401 catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc tttatgggat
     5461 tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa atggtgccct
     5521 ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc acgtcatttc
     5581 tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt tcaaataccg
     5641 ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg tttgcactgt
     5701 tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg gcggtactgg
     5761 cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct caggcgggtt
     5821 accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt attgctgtga
     5881 ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga tcagatagcc
     5941 tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct cttaatgcag
     6001 gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac gccccggttt
     6061 ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca tccagtatgc
     6121 atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg cttctggcga
     6181 cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg cctccagcgc
     6241 tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc gcatatagcc
     6301 aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca agccgaataa
     6361 cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca tatgttgaaa
     6421 agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca catagtcagc
     6481 cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag aactcaacac
     6541 cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag tgttgtattt
     6601 ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg ttttcatacg
     6661 cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc gtatcagaga
     6721 tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg acaagttgca
     6781 ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt gattttattc
     6841 ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga
     6901 ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa gtggaggcgc
     6961 gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg ctttgttaac
     7021 gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt cttttaaact
     7081 ggagcgggaa acgagactcg aactcgagac cccgaccttg gcaaggtcat gctctaccaa
     7141 ctgagctatt cccgtattca tcaagcaatc agttaatcac ttgattttat tatcgtctgg
     7201 caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg agtcaacgat
     7261 atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac tgatcaagca
     7321 aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt accgcagcca
     7381 caaagaaaag tgcaataccg gcgtactcaa cccaaatgtt cggacgccac agcagccagg
     7441 ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg gccacgctac
     7501 tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt tcacgggcga
     7561 tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt accagcacca
     7621 tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca aaccgggtac
     7681 tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga
     7741 aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc aatacaaaga
     7801 atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat tgcataatga
     7861 cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac atcaatgttt
     7921 caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt ttgcaatttc
     7981 ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca acatttgccg
     8041 acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga ccttcgcccg
     8101 ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat gctggataac
     8161 atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct caaagaacag
     8221 cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca gcggatgatt
     8281 tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa gctggccttt
     8341 gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt taccataacg
     8401 ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc ctgtaatgtt
     8461 atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac aggaagcgac
     8521 ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca cttccggcaa
     8581 tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct gcgaaagttt
     8641 gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg ccctatcgcc
     8701 gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg aatcggcgag
     8761 cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg tgcgcatctg
     8821 gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca gttccgtacc
     8881 gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac gaatgaacaa
     8941 tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa ggtcgtcgcc
     9001 ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat
     9061 acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc taatgagttg
     9121 cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga cctgctgagc
     9181 gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc ctatctggta
     9241 ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc gaatggggaa
     9301 aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga acgggccgaa
     9361 atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt gggtatcgcc
     9421 actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt aacgcggctg
     9481 atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat gcgtaaccgt
     9541 tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct
     9601 gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat agataaccgt
     9661 accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt ttaaaaacgc
     9721 ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca cagaccatgg
     9781 cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact gaacatacga
     9841 tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat ctcattgacc
     9901 ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa actggcaaat
     9961 gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc gatatcagaa
    10021 gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc ctgcggagcc
    10081 gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg taaagggttt
    10141 tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc gattttacgc
    10201 gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac gtcaacggca
    10261 tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac gacttttata
    10321 ccctttatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca
    10381 agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact gatagttagt
    10441 cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt gggtattcga
    10501 tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc gtcaccaacg
    10561 gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat tgactttatt
    10621 cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga acaatacggg
    10681 caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt ttatagcact
    10741 gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat ggtatactcc
    10801 gccgccttaa catttttcac cgcaaatttt cattgcaaca tgacgaggaa aataaatgag
    10861 tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag tctcctgcct
    10921 gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg gccgcgtgat
    10981 gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg cagtattttc
    11041 caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg gctgagagcg
    11101 tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct tcccttccgc
    11161 cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg tcatcactat
    11221 ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact gcatttttaa
    11281 ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa aggacaaaaa
    11341 gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta aaccatgtgc
    11401 gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc acattaaatg
    11461 gtcctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat tttttgcctg
    11521 ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag gtcgcgtgaa
    11581 tagtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct gagcctgaag
    11641 ctctatttca tggtagacct cttctgcggc ctccatcctc tgaaaacgca acagcatcgt
    11701 gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc ctgagagtga
    11761 gtatcattta taatgataat tcaaactatc acataaataa gatttatata taatttatat
    11821 tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat taatgagtta
    11881 tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg acaggtgaat
    11941 cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac tggcgggtgc
    12001 gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact atccgcccct
    12061 ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg tgtgtcacaa
    12121 tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact tcgcccacca
    12181 gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga cgcattgcca
    12241 gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt ggatagctgg
    12301 tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc gtggcctctt
    12361 ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc accgtacgat
    12421 gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac tggcgaatca
    12481 gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca acggtgatcg
    12541 ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt ttgccggaac
    12601 cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg ataccgtgca
    12661 gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca ctcattttgg
    12721 ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg ataacacagt
    12781 cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct ccagcgtgcg
    12841 cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg cggccagcga
    12901 cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca cccgcgccgc
    12961 ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac tggcagcggc
    13021 ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg cggcataggc
    13081 ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt cgataccaaa
    13141 ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac cacgaaaaat
    13201 agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc gcatcagcgc
    13261 gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt gcagggtata
    13321 cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac tttcttgcat
    13381 gagcgttccc ggagttaaac gtggggatga taggcgaaca gcgcaggcgc gccgccggta
    13441 tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc gtcaatcagc
    13501 cccgccatcg cttttccggt atacacagga tcaagcagaa taccttccag acgcgccagc
    13561 aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc aaaatagtca
    13621 tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat cgcctgttgt
    13681 aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac gccaatcagt
    13741 tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt tccggcactg
    13801 cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg cgcgatttcc
    13861 agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac cggaatgaca
    13921 tacgggcgaa agccttgtgc ttcgactcgc gtcgccagct cttccagttg ggcattggga
    13981 tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa caaacgattg
    14041 ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag cgccacgcag
    14101 tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga ctggatcgcg
    14161 ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag aaattccagc
    14221 ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt gatgaaaatt
    14281 tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg cgcgccgata
    14341 aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc cgatgcgtgt
    14401 tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc gctagtgaaa
    14461 gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac catttttcgg
    14521 aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc accgctttca
    14581 gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag aatgcttcac
    14641 cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc agacgatcaa
    14701 cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg tcatcatcat
    14761 aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag ttggtgccca
    14821 gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg ccttcgttac
    14881 cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa tcgtattttt
    14941 tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt ttagagtcca
    15001 gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg ccaagatgtt
    15061 ttgccagctg ttgagcaaat tccacttcaa aaccggttaa tttgccgtca tccccctgaa
    15121 aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgcgc tctttgactt
    15181 tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc agcgccacgc
    15241 ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc accccgaatg
    15301 ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca actccgctac
    15361 atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc tgcacaagtc
    15421 cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg taattgttgg
    15481 tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc tgcagctgat
    15541 cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga acgacatatt
    15601 cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta aagccgataa
    15661 tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg cacaatgccg
    15721 cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc aggcgggaaa
    15781 tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg agtaatttac
    15841 ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg cttaatggcc
    15901 gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta aagtgcggca
    15961 tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatggcc tggctgtgta
    16021 actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg agattcagct
    16081 cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt tcgatggctt
    16141 ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt tctcgctgat
    16201 gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca tcgtaggaga
    16261 agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata tcgatcccta
    16321 aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc agttgcccta
    16381 ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag tcacggctgc
    16441 gaagttcatc cagcatagcg ccacggatac gctgcactgc gtaagttgta aatgccgttc
    16501 cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc gcctgtagca
    16561 gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct tcgtgacaca
    16621 ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca gcggtataga
    16681 gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta tccgtttctg
    16741 cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg gttacgcaaa
    16801 aagccgaatg taaaaaaccc cgtcggtggc ggggtttgag aggcattcag acgattaacc
    16861 ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca cggagttacc
    16921 ggcctgcagg ataatctgcg ctttcgacat gttggacact tcggtcgcat agtcggcgtc
    16981 ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt tggtgactgc
    17041 ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga tggagctgat
    17101 ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt cagcatctgt
    17161 cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag tcactgaacc
    17221 atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt tgttatcaac
    17281 ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc tataacctac
    17341 agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag ctgcctgatc
    17401 aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat taccggtaaa
    17461 ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg ctgcaagtgt
    17521 ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc gcccagttgt
    17581 atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc tgatctgaac
    17641 atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa ctcccggggt
    17701 caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt atgacttact
    17761 tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt tattaatggt
    17821 tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt tagcagcatc
    17881 gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag ctaccaagtc
    17941 agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa acccattcag
    18001 ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc cgtcattcgc
    18061 accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt tgaactgggt
    18121 ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct gaatggagtc
    18181 cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac ggatacgctg
    18241 taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag aaataccgtc
    18301 gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac ggttagcaat
    18361 cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag acagacgctc
    18421 gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga tcagcgagag
    18481 gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag tcgttgatta
    18541 cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt ctgtcgtaaa
    18601 acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga aatactcttt
    18661 tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt gcgcaattca
    18721 ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc gcatggcaag
    18781 tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg atagcctcac
    18841 cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt ttaccgctaa
    18901 acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg ccaatactgc
    18961 attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca ccgcgttcag
    19021 tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca cccatctggc
    19081 gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg cgatcgccac
    19141 cagcgacagc aaactcacca ttcaacaagg cggcgacaaa gatccgatta ccattgatat
    19201 cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg caaaagcggg
    19261 cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag tcacatcaaa
    19321 tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg cgctacaaag
    19381 ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg tcgccgccca
    19441 gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca ctatcagcga
    19501 cgcgctggaa aacatcaccc tgaacctgaa cgatgtcacc acgggcaacc agacgctaac
    19561 catcactcag gacacctcca aagcgcaaac ggcgattaaa gactgggtga atgcctataa
    19621 ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg cgggagctga
    19681 tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga cgattcagac
    19741 gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa cgctggcgca
    19801 gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg acaaactcac
    19861 cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg atggtaaaaa
    19921 aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga caacgggcat
    19981 tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta aagactacaa
    20041 cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat ttacccaact
    20101 ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc agttcgaaaa
    20161 caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg cggcaaaagg
    20221 cacccaggcc tatgcgcaaa ttggcgtcga aagcaccgta atgagcgcca gccaacagca
    20281 gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc gactgtttat
    20341 gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca acatcattga
    20401 gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc aaaacttaat
    20461 tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca acgatgtctc
    20521 cgcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga aagagtcttt
    20581 actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca tttgtatttc
    20641 gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac ggaagaacaa
    20701 tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga gatcgcacac
    20761 ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc gatgctgcac
    20821 ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat ggatgaactg
    20881 gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta tggcgatcag
    20941 ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag ccgatggctc
    21001 gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt cgaagaatgc
    21061 gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt aagctctggc
    21121 ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg gtctggttgc
    21181 cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc tatgatttat
    21241 ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt gataaagcac
    21301 aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg ctggacgtgg
    21361 tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag cgtgtaaatg
    21421 ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg acgcgttaca
    21481 ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa tgttttagcg
    21541 gtatcgacca tatcgaacac cctgatgaag attgcatttt taaaattgtt aacgactata
    21601 ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat tatctgatgg
    21661 gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac tgggcgcgct
    21721 gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa catattccgg
    21781 catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag ccgctgttta
    21841 ttgtggctga atactggtcg catgaagtcg ataaactgca aacgtatatt gatcaggtgg
    21901 aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa gcatcgcgca
    21961 tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa gccgatcctt
    22021 ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc ctcgaagcac
    22081 cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg gaaaatggcg
    22141 ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc ggtggtgacg
    22201 ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta attctcgccc
    22261 gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac tgcattgcct
    22321 ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg aacggagatg
    22381 atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg cgtgattttc
    22441 tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc ttcttttgca
    22501 acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat ccccggcggc
    22561 aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc cagcgcggcg
    22621 gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta ctcaatggtg
    22681 ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt gagatcatca
    22741 gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg gcaatccaag
    22801 gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac cagcgcacgt
    22861 tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt tttcacgaca
    22921 ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc tgcaattgcg
    22981 agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt aaagctctga
    23041 tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt ttgtgaatta
    23101 ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca aaccttctga
    23161 gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg attaaattct
    23221 gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat tttggcatta
    23281 ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag ctcctgcagc
    23341 tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg gaaggatcgc
    23401 cattaacccg catcgacggc atgatgatcc tcggtatgtt tggcggctgc tttgctgctg
    23461 cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc attatgcagg
    23521 ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc tgtaacctgg
    23581 cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt gccatcgcca
    23641 ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc cgtattcccg
    23701 tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat caggcgcggc
    23761 gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg ctgctcacgg
    23821 cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt ggtttactga
    23881 ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc accggacgta
    23941 cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg atcttcagtt
    24001 acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg gtaattggtg
    24061 gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc tggatgtacc
    24121 gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg atcggctcaa
    24181 cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac tgggacaaaa
    24241 tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat ttgctgctgt
    24301 ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt gcggcaccgc
    24361 agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg atatggtggg
    24421 tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat taaaaaaagg
    24481 tgagatccta gaagtggtga gcgactgtcc gcagtcgatc aataatattc cactggatgc
    24541 gcgtaatcac ggctacactg tgctggatat tcagcaagac gggccgacca ttcgttattt
    24601 aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg ttacctctga
    24661 cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag gaggttgcta
    24721 tgtgtggacg ctgtgcccaa tcccaaacgc gtgaggatta ccttgcgctt ctcgcggaag
    24781 atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat gtcgcgccgg
    24841 gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat ccggttttct
    24901 ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc gtagaaactg
    24961 cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc tgttttgccg
    25021 atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta tctatcgcgc
    25081 tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc gtggtgacga
    25141 agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag atattcatga
    25201 ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc aagacattgg
    25261 tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc agttcacctg
    25321 gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt taattcaacc
    25381 tgtttgttga catcctccac gccctgaatg acgaggcccc ctgctacgtt caggctgtcg
    25441 cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc atctctccac
    25501 aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc gccatttaca tcggcgttcg
    25561 ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt tctttcgctg
    25621 tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg cccgccacgc cagagctgct
    25681 tgtactcaag ctggcggcgc atttcatacc agccctgatc cagtatcgaa cggtttaaac
    25741 ctgattttgc ccggacattg cgccccggct ggctgaccgt acccgctgct gactttgaca
    25801 tgttgctgac cttcaaatcc tcaatgacta tcattacgtg gtttttgctg acggtcgttg
    25861 tgactttgtg aaggtagtcc ctgcggatat ttgcgataca ggaatgcagt cgctgtattt
    25921 tgcgtttctg cttctgccag ttgttgctga atttgacctt gcggcttaac tgtcgctgaa
    25981 gtctcgccag
//
LOCUS       DAJVQH010000047        25884 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJVQH010000047 DAJVQH010000000
VERSION     DAJVQH010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25884
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.047"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            346..639
                     /locus_tag="OQM87_002020"
     CDS             346..639
                     /locus_tag="OQM87_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCS1508165.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            711..1370
                     /locus_tag="OQM87_002021"
     CDS             711..1370
                     /locus_tag="OQM87_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCS1508166.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1462..1908
                     /locus_tag="OQM87_002022"
     CDS             1462..1908
                     /locus_tag="OQM87_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCS1508167.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2115..3015)
                     /gene="hlyE"
                     /locus_tag="OQM87_002023"
                     /pseudo
     CDS             complement(2115..3015)
                     /gene="hlyE"
                     /locus_tag="OQM87_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3388..3807
                     /locus_tag="OQM87_002024"
     CDS             3388..3807
                     /locus_tag="OQM87_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCS1508168.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQRL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3807..5073
                     /gene="umuC"
                     /locus_tag="OQM87_002025"
                     /pseudo
     CDS             3807..5073
                     /gene="umuC"
                     /locus_tag="OQM87_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(5119..5649)
                     /gene="dsbB"
                     /locus_tag="OQM87_002026"
     CDS             complement(5119..5649)
                     /gene="dsbB"
                     /locus_tag="OQM87_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCS1508169.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5795..7336)
                     /gene="nhaB"
                     /locus_tag="OQM87_002027"
     CDS             complement(5795..7336)
                     /gene="nhaB"
                     /locus_tag="OQM87_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCS1508170.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7557..8276
                     /gene="fadR"
                     /locus_tag="OQM87_002028"
     CDS             7557..8276
                     /gene="fadR"
                     /locus_tag="OQM87_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCS1508171.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8328..9860)
                     /locus_tag="OQM87_002029"
     CDS             complement(8328..9860)
                     /locus_tag="OQM87_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCS1508172.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHTCYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10190..11488
                     /gene="dadA"
                     /locus_tag="OQM87_002030"
     CDS             10190..11488
                     /gene="dadA"
                     /locus_tag="OQM87_002030"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCS1508173.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11498..12568
                     /gene="dadX"
                     /locus_tag="OQM87_002031"
     CDS             11498..12568
                     /gene="dadX"
                     /locus_tag="OQM87_002031"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCS1508174.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12736..14162)
                     /locus_tag="OQM87_002032"
                     /pseudo
     CDS             complement(12736..14162)
                     /locus_tag="OQM87_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14772..16508)
                     /locus_tag="OQM87_002033"
     CDS             complement(14772..16508)
                     /locus_tag="OQM87_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCS1508175.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16603..17517)
                     /gene="ldcA"
                     /locus_tag="OQM87_002034"
     CDS             complement(16603..17517)
                     /gene="ldcA"
                     /locus_tag="OQM87_002034"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCS1508176.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17617..18228
                     /gene="emtA"
                     /locus_tag="OQM87_002035"
     CDS             17617..18228
                     /gene="emtA"
                     /locus_tag="OQM87_002035"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCS1508177.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18230..18964)
                     /locus_tag="OQM87_002036"
     CDS             complement(18230..18964)
                     /locus_tag="OQM87_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCS1508178.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19165..19419
                     /locus_tag="OQM87_002037"
     CDS             19165..19419
                     /locus_tag="OQM87_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS1508179.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19469..20929)
                     /locus_tag="OQM87_002038"
     CDS             complement(19469..20929)
                     /locus_tag="OQM87_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1508180.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20866..21423)
                     /locus_tag="OQM87_002039"
     CDS             complement(20866..21423)
                     /locus_tag="OQM87_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCS1508181.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARVLTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21449..22303)
                     /locus_tag="OQM87_002040"
     CDS             complement(21449..22303)
                     /locus_tag="OQM87_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCS1508182.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22303..23112)
                     /locus_tag="OQM87_002041"
     CDS             complement(22303..23112)
                     /locus_tag="OQM87_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCS1508183.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23122..23880)
                     /locus_tag="OQM87_002042"
     CDS             complement(23122..23880)
                     /locus_tag="OQM87_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1508184.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23877..24857)
                     /locus_tag="OQM87_002043"
     CDS             complement(23877..24857)
                     /locus_tag="OQM87_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCS1508185.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISP
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24857..25879)
                     /locus_tag="OQM87_002044"
     CDS             complement(24857..25879)
                     /locus_tag="OQM87_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCS1508186.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6447 a         6371 c         6727 g         6339 t
ORIGIN      
        1 tggcctttgc tgaactcaac attgacattc ttacccgcag caaagctcgt ggaggttagc
       61 agtgcaagca gcaatacagc cttactgatg ctcttgattt tcattggtag tctcctgttc
      121 agaatgagta cgatgaactg ttataatata acaatccctt acgggagaat tccagtggct
      181 tttctgcgtt cggtaaaatc ccggcgcggc attttttgcc ggggggatta cttataaagg
      241 ggcatggact caacaaaccg cagaaatcta cagctggctg gcttgtggcg cgggtttcat
      301 tgatgttagc ttatgccaaa acctggcatc ttaaagagta aaagtatgtt ttgtgtgatt
      361 tatcgaagca gcaagcgtga ccagacctat ttatatgtcg aaaaaaaaga cgatttttcg
      421 cgtgttcctg aggaactgat gaaaggtttt ggtcagcctc agttagcgat gattctgccg
      481 ttggatgggc ggaagaaact ggtcaatgct gatattgaaa aggttaaact ggcattaacc
      541 gagcaaggtt actatttgca gttaccgcca ccacccgaag atttgctgaa gcaacatctt
      601 tccgtcatgg ggcagaaaac agacgacact aacaaataac cgatatccgg cggtggcatt
      661 atctttgtcg gcgcgggttt tcatatccac gataaggtga ggggaacgtt atgtatcaac
      721 atcacaactg gcaaggtgcg ctgctggatt atccggtgag taaagtagtc tgtgttggca
      781 gtaactatgc caaacatatt aaagagatgg gcagcgcagt gcccgaagag ccagtgctgt
      841 ttattaaacc agaaacggca ctgtgcgatc tgcggcagcc attggcgatc ccatccgatt
      901 tcggttcagt tcatcatgaa gtcgaactgg cggtgttgat tggcgcgacg ctgcgtcagg
      961 caacggaaga gcatgtccgc aaagccattg ccggttacgg cgtggcgctt gatctgacat
     1021 tgcgtgatgt tcagggaaaa atgaagaaag ccgggcagcc gtgggaaaag gctaaagcgt
     1081 ttgataactc ttgtccgctt tccgggttta tccccgcggc ggaatttacc ggcgatccac
     1141 aaaatacaac gctgggcctg agcgtaaacg gcgaaaaacg ccagcaaggt acgactgcgg
     1201 acatgatcca taaaatcgtt ccgttgatcg cttatatgag caagtttttt accctcaagg
     1261 ccggtgacgt tgtgttgaca ggcacgcctg atggcgtcgg cccgttgcaa agcggtgatg
     1321 agctgacagt cactttcgat gggcattctt tgacaactcg cgttttgtaa tactttttgc
     1381 cgccagaaag cggcggcaaa acttgcatcg ctgtgccaga ctggttataa ggtgcgtttt
     1441 aacgtaatgg cggaacacct gatgagcgat gtacctttct ggcaaagtaa aaccctggac
     1501 gaaatgagcg atgcggaatg ggagtcgttg tgtgatggtt gcggtcagtg ttgcctgcat
     1561 aaactgatgg atgaagacac cgacgaaatc tacttcacta acgtcgcctg tcgccagctc
     1621 aatattaaaa cctgtcaatg tcggaactac gaacgtcgtt tcgagtttga acccgactgc
     1681 attaaattaa cccgtgaaaa tctgccaaca ttcgaatggc tgccaatgac ctgcgcttat
     1741 cggttgctgg cggaaggtaa agatttacct gcgtggcatc cgctacttac tggttcgaaa
     1801 gcggcaatgc atggtgaacg tatctctgtg cgtcatatcg cagtgaaaga atcagaagtg
     1861 attgactggc aggatcatat cttaaataaa cctgactggg cgcagtgata aatttaatag
     1921 atgctgatgg gggctttctt aatatacgtc tggatgctcg ccatttcgta gccctgtttt
     1981 tgaaagcgct ctgtcataat gagagttacc cgatacctta ttatcaacag gtaactctca
     2041 atctttaaaa aacgtctgtg tttttaaatg tgataaacct tataccttga gtacatggag
     2101 agaataatcg cttatcagac ttcaggtact tcaaagagtg tctttttacc gtgtcttttc
     2161 tgatactcat tacaggtgtt aatcattttt ttggccgctt cttttagcaa agaaagcatt
     2221 aaatcatcat aatcaacata gaatctggtt gtttcagttt ccgtttttat ctccccgatg
     2281 gcggctattt cggtggttaa tttcaatttg gcggcatcga tatctttatt cgcttgttta
     2341 accgtgttag acagggtggt aaagaaactc tgcacagatt ttaacttgtt cttcaattct
     2401 ggaatcagtt tcccttcaac tacgcccgca gcaatagaat aggaaatgat taaaccaaat
     2461 ggaccggcga cgacatcggc tgcggcaccg gcatacgctt ccttcctgat tttatctacc
     2521 tgtgactgga aatagctgct tttttctgaa aaatcattgg ttaactggct atctaacgcc
     2581 agcagtttcc cggaagcgtt gttgaaactt tgtgagctta ccagcaggga tttttgcgct
     2641 tcattcagct tcgtgatgcc gtcatccagt accttaatga ggggcggatg ctttcttctc
     2701 attgtactca tcaaatagca aaatatacgc tgcgagcaat tgcgtcgcaa caccacacca
     2761 ttcatacact gtttgggttg cttcaaaata cttatcctgg ctatccataa gtaaggtttt
     2821 aatatcgccg actaaaacgg aggctgcctg tgaatactcc tgtttaaagc gacttaactc
     2881 ttttatggtt tcatcaaagg tctgccaggg gatgacctga tcgagatatt tattataaag
     2941 atctaatgct ccatctgcgg tttcgattgc gtttttaact acttctaccg ttttatctgc
     3001 aacgatttca gtcataatca ttcgcctctt taaatatata aattgtaatg aaactcctgt
     3061 tttacaacta ttaataaatt ttacttcatc taattcatag ttagccgggc gggatgcgtc
     3121 aatgtcttta tttctattaa tatgataaat atcaaacaat gtttaatgtc attatggcga
     3181 atgcttctat tctatttttt tgccgggtga tatttttcat ttctgctgga tgagcgtcgt
     3241 cgccagaagg ccacgtgagc acaggataag agaacgaaaa atcagcagtc tatgcagcga
     3301 caaatattga tagcctgaat cagtattgat ctgctggcaa gaacagacta ctgtatataa
     3361 aaacagtata gcttcaggca gattattatg ttgtttatca agcctgcgga tctccgcgaa
     3421 attgtgactt ttccgctatt tagcgatctt gttcagtgtg gctttccttc accggcagca
     3481 gattacgttg aacagcgcat cgatctgaat caacggttga tccagcatcc cagcgcgact
     3541 tacttcgtca aagcaagtgg tgattctatg attgatggtg gaattagtga cggtgattta
     3601 ctgattgtcg atagcgctat taccgccagc catggtgata ttgtcatcgc tgctgttgac
     3661 ggcgagttta cggtgaaaaa attgcaacta cgcccgacgg tacagcttat tcccatgaac
     3721 agtgcgtact cgcccattac catcagtagc gaagatacgc tggatgtctt tggtgtggtg
     3781 atccacgtcg ttaaggcgat gcgctgatgt ttgccctctg tgatgtaaac gcgttttatg
     3841 ccagctgtga gacggtgttt cgccctgatt tatggggtac cggtggttgt gctatcgaat
     3901 aatgacggtt gcgttatcgc ccgaaacgct gaggcaaagg cgctgggcgt taaaatgggc
     3961 gatccctggt tcaaacaaaa agatctgttt cgtcgctgtg gtgtggtttg ctttagcagc
     4021 aattatgagc tttacgcaga catgagcaat cgggtgatgt cgacgctgga agaactatcg
     4081 ccccgcgtcg agatttacag tattgatgaa gcattctgcg atctgacagg tgtgcgtaat
     4141 tgtcgcgatc tgactgattt tggcagagaa attcgcgcaa cggtgctaca acgtacccat
     4201 cttactgttg gtgtggggat cgcccagacc aaaacgttgg ctaagcttgc caatcatgcg
     4261 gcaaaaaaat ggcagcggca gacgggtggg gtggtggatt tatcaaatct ggaacgccag
     4321 cgtaaattaa tgtctgctct ccccgtggat gacgtctggg ggattggacg gcggatcagc
     4381 aaaaaactgg acgcgatggg gatcaaaacc gttctcgatt tggcggatac agatatccgg
     4441 tttatccgta aacattttaa tgtcatgctc gaaagaacgg tgcgtgaact gcgcggcgaa
     4501 ccctgtttgc aactggaaga gtttgcaccg acgaagcagg aaattatctg ttcccgctcg
     4561 tttggtgaac gcatcacgga ttatacgtcg atgcggcagg ccatttgtag ttacgctgcc
     4621 cgggcggcgg aaaaacttcg cagtgagcat caatattgtc ggtttatctc cacatttatt
     4681 aagacgtcac catttgcgct caatgaacct tattacggca atagcacgtc ggtaaaactg
     4741 ctgacgccca ctcaggacag cagggatatc attaacgctg ctacgcgatc tctggatgcc
     4801 atctggcaag cgggccatcg ctaccaaaaa gcgggcgtga tgctggggga tttcttcagt
     4861 cagggagtcg cgcagctcaa tttattcgat gacaatgcac cgcgccccgg gagtgagcaa
     4921 ttgatggcgg taatggatac tctgaatgct aaagagggca gaggaacact ctattttgcc
     4981 gggcagggga tccaacagca atggcagatg aagcgagcta tgctttcacc acgttataca
     5041 acgcgaagtt ctgatttact gcgggtcaaa taaatatagc ggcaggaaaa aagcgttccc
     5101 gcaggagcgc cgaatggatt agcgaccgaa cagatcacgt tttttcgctt taaacggctg
     5161 ggaaatcacc accagcaccg cgacaatcag gtaagcgata aaaataccga gcagccactg
     5221 cggcatttcc atacctaaaa aatcccactg acgctcggcg caatcgccag aggcgacaaa
     5281 cacttgcggc acccatttat ccagcggcag ccattccggg aaacgaacca taaaatcaca
     5341 ggtggcaaat ggcgaaggat agagctgaag catggtgtgc tcgtaagtta actgcacacc
     5401 gcggaacgca ctatacaacc agataaccat cgctacataa cgcagcggag ttttcggggc
     5461 gatcgcgcca atcagcgcag cacccagaac gccgaataac gcgcagcgtt cataaataca
     5521 gagcacgcaa ggtttcagta acatcacatg ctggaaccac agcgccgtca gttccagtgc
     5581 cagagcagta aacgccatca acagccacgc gccccggcct tgtgaacatt ggttcaaaaa
     5641 tcgcaacata atcatttccc tgcaatatgc atagagtgcg cagtttaaac caattcattc
     5701 gctgcgccac caggaggcag caaaaatagg acgcaattgg acaattatcg ggcgaaaagg
     5761 caaaccgggc atttatatgc ccggtaagtt gttatcaaag cgttgctatc cagcccattt
     5821 gcataaacca ttcggttaca ggggcaagcg taaactcgac gcagagcaag ccgacgagtg
     5881 tcagtacgag ggtgtaaggc agggccatcc acaccatgcg gccataagag aggcgaatca
     5941 atggcgcgag tgcagaggtc agcaggaaca ggaacgcagc ctgaccgttc ggcgtagcga
     6001 cagagggcag attggtaccg gtattaatgg cgaccgccag cagctcgtat tgcttcaacg
     6061 tgatagcgcc actttccatt gccgctttcg cttcgttgat ataaatcgtc cccacgaaga
     6121 cgttatccga aatggatgac agcagaccgt tgaaaatata gaacagcgac agctgagcat
     6181 gttcagatgc ctgcaacaca aactgaataa tcggcgaaaa cagttgttgg tcgataatca
     6241 ccgcgactac cgagaaaaac accgtcaaca gtgcggtgaa tggcagagat tcggtgaagg
     6301 ctttaccgat agcatgttca tcggtgacac cggtcaatga tgttgccaga ataatgactg
     6361 acaaaccaat caagccaact tccgccagat gcaacgccag cgcagtcacc agccagacgc
     6421 caataatcgc ctggacaatc agacggattt tatcctgacg ggtacgctgg tggcggcttt
     6481 gatcgtcaaa ctgttgcaac acttcgcgga ctttctccgg cagcgtttca ccgtagccaa
     6541 accaacgcag cttctctacc agcaggcagg ttaacaggcc acaaatcaga accggaacgg
     6601 tcaccggcga catgcgcagg aagaaatcgc caaaatgcca gccagccgct ttagcgatga
     6661 tcaggttctg tggttcgccc accatggtca ttacgccgcc taatgcggtg ccgacaccgg
     6721 catgcatcat caggctacgc agaaagccac ggaactgttc cagaaccacg ttgtaatgct
     6781 tgtcgatatg actatcgtct tgcaggtcgg tgtcttcggt acgggaagag gctacgcgat
     6841 gataaatacc ataaaaaccg actgcaacgc tgatcaccac cgccacgacg gttaaggcat
     6901 cgaggaacgc ggagaggaac gctgccgcca cgcaaaaaga gagcgacagc agcattttgg
     6961 agcgaatgct taacagcaaa cgggtaaata tgaacagcaa cagctgtttc ataaaataga
     7021 tacccgccac cataaacatc aataacagca agacttcaag atttgccgcc accccttcac
     7081 ggacgtgttc cgcgctggtc atgccgatga ataccgcttc gatagccaac agaccaccgg
     7141 ggagtagcgg gtagcatttc agggccatcg ccagagtgaa aataaattcc gctaccagca
     7201 accagcccgc gacgaaaggg ctgatgagga aaattaacgg gtttacgatt aagaaaatga
     7261 tgagggcgag tttgtaccag tcgggggact ggcccaaaaa attgcgccat agcgcgcggc
     7321 cccaggagat ctccatgatg gtttccctta ccttacaaat aatcaatgat gtttttgtgt
     7381 ttaaacgcaa agcttaacgg tcaggcagga gtgaggcaag tcttgatagt caaggggaaa
     7441 gagatgcgga aaatgaagcc ttgatccctt tttcttcttt ttgtctgcta tcagcgtagt
     7501 tagccctctg gtatgatgag tccaactttg ttttgctgtg ttatggaaat ctcactatgg
     7561 tcattaaggc gcaaagcccg gcgggtttcg cggaagagta cattattgaa agtatctgga
     7621 ataaccgctt ccctcccggg actattttgc ccgcagaacg tgaactttca gaattaattg
     7681 gcgtaacgcg tactacgtta cgtgaagtgt tacagcgtct ggcacgagat ggctggttga
     7741 ccattcaaca tggcaagccg acgaaggtga ataatttctg ggaaacttcc ggtttaaata
     7801 tccttgaaac actggcgcga ctggatcacg aaagtgtgcc gcagcttatt gataatttgc
     7861 tgtcggtgcg taccaatatt tccactattt ttattcgcac cgcgtttcgt cagcatcccg
     7921 ataaagcgca ggaagtgctg gctaccgcta atgaagtggc cgatcacgcc gatgcctttg
     7981 ccgagctgga ttacaacata ttccgcggcc tggcgtttgc ttccggcaac ccgatttacg
     8041 gtctgatcct taacgggatg aaagggctgt atacgcgcat tggtcgtcac tatttcgcca
     8101 atccggaagc gcgcagtctg gcgctgggct tctaccacaa actgtcggcg ttgtgcagtg
     8161 aaggcgcgca cgatcaggtg tacgaaacag tgcgtcgcta tgggcatgag agtggcgaga
     8221 tttggcaccg gatgcagaaa aatctgccgg gtgatttagc cattcagggg cgataatccc
     8281 ttccgtttaa agagcaaacc cctcaaacga ggggtttttt gttgttttta cagatttccc
     8341 attcttggcg ggcaacgttc cagcaactcg atgctgccgt cttcgttttg ctgttcgagc
     8401 atcacatcaa atccccacag gcgatgcaca tgcttcagga cttctttgcg cccccgatcc
     8461 agcggtgcgc gattatgtgg aatataacgc agcgtcagcg aacggtcgcc gcgcaaatcc
     8521 acgttccaga tctgaatatt cggctccaga ttacttaagt tatattgcga cgataaccgg
     8581 ttacggatct cccgataacc ttcttcatta tgaatagcgg aaatctccag ataattatgc
     8641 cgatcatcat ccagcacggt gaagaagcgg aaatcacgca tcactttcgg tgacagaaac
     8701 tggctgataa aactctcatc tttgaaatca cgcatcgcga aatgcagcgt ttccagccag
     8761 tcggaaccgg cgatatccgg gaaccagtat ttgtcttctt ccgttggcga ctgacaaatc
     8821 cgtttaatat cctggaacat ggcgaacccg agggcatacg ggttgatgcc gctgtaccac
     8881 gggctgttat aggggggctg gaagaccaca ttggtgtggc tgtgcaaaaa ctccagcata
     8941 aaacgttccg ttactttccc ttcatcatac agatggttaa ggatggtata gtgccagaag
     9001 gtcgcacagc cttcgttcat aacctgagtc tgtttttgcg gataaaaata ctggctcacc
     9061 ttacgcacaa tacgcaggat ttcacgctgc catgattcca gcagtggcgc atttttctcc
     9121 ataaaataga gcaggttttc ttgtggttcg gacggatagc ggcgcgcttc agcaaccgtt
     9181 ttctcttcct cgcgcttcgg cagggtacgc cagagcatat tgacctgact ttgtagatac
     9241 tcttcgcgac ttttctgccg ggctttctct tcttgcagcg agattttttg cgggcgtttg
     9301 taccggtcca cgccgtagtt catcagcgcg tggcacgagt ccagaagccg ttctacttca
     9361 tcaacgccat aacgttcttc gcactcggta atatatttac gggcaaaaat cagataatcg
     9421 acaatcgaac tggcgtcggt ccaactgcgg aataagcaat tgtttttaaa gaaagagtta
     9481 tgcccatagc aggtgtgtgc catcaccagc gcctgcatag taatggtgtt ctcttccatc
     9541 aagtaagcga tacacgggtt agagttaatg acgatttcat aggccagccc ttgctgaccg
     9601 tgcttataca gccgttcagt ctcgataaac tttttaccga atgaccagtg cggatagtta
     9661 attggcatgc cgacgctgga ataggcatcc atcatctgtt ctgaggttat cacttcaatc
     9721 tggtgcgggt aagtatccag ccggtagagt ttcgccaccc ggtctatctc tgccagataa
     9781 acatccagca ggtcgaacgt ccagtcgggt ccatcgctca aacgtgtggt gtccttatta
     9841 atagaatcga tcgtcgccat acgcgcacct cattgttgtc ggcgctctct gtgtggagca
     9901 cctcatttca agcatagaac acctgttaaa aaccgcgtcg ccggagaatt tttttctttg
     9961 cgatttctta ttatcagagt gccactaatc cgcttctgaa cggaatttta tgctggataa
    10021 aaagggcgtt cagcaggaga tactaaagac gccatattgc cgcagagtca gggagatgtg
    10081 agtcagctca ccataaaaaa gccgtatgtt gaataatatt ttcaactgag ttatcaagat
    10141 gtaattagat tattattctt ttactgtatc taccgttatc ggagtggcta tgcgagttgt
    10201 catactggga agtggtgtgg taggcgttgc cagcgcctgg tacttaaatc aggcaggaca
    10261 tgaggtcacc gtcattgatc gggagccggg ggccgccctg gaaaccagtg ccgcgaatgc
    10321 cgggcaaatc tcccccggat atgctgcacc gtgggcagca ccaggtgtgc ctttaaaagc
    10381 gattaaatgg atgttccagc gccatgcgcc gctggcggtt cgtctcgacg gtacgcagtt
    10441 tcagttgaaa tggatgtggc aaatgttacg taactgcgac accagccact acatggaaaa
    10501 caaagggcgg atggtgcgtc tggcggaata cagccgtgat tgcctgaaag cattgcgcgc
    10561 cgaaaccaat attcagtatg aagggcgtca gggtgggacg ctgcaactgt tccgtaccga
    10621 acaacagtat gaaaatgcga cccgcgatat cgccgtgctg gaagatgccg gcgtaccgta
    10681 tcagctgctg gaatccagcc gcctggcgga agtggatccc gcgctggcag aagtagcgca
    10741 caaactgacg ggcggcctgc agttacccaa tgatgaaacc ggagactgcc agctgtttac
    10801 ccagaatctg gcgcggatgg cagagcaggc gggggttaaa ttccgcttta atacgcccgt
    10861 tgaccaactg ctttgcgacg gcgagcaaat ctacggcgtg aagtttggcg atgaagtgat
    10921 taaggccgat gcgtatgtga tggcgtttgg ttcttactcg acggcgatgc tcaaaggcat
    10981 tgttgatatt ccggtgtatc cgctgaaagg ctactcgctg actattccaa ttgcgcagga
    11041 agatggtgcg ccagtatcca ccattcttga tgaaacctac aaaatcgcca ttacccgttt
    11101 cgataaccgc attcgtgttg gcggaatggc ggaaattgtt ggttttaaca ccgagctgtt
    11161 gcaaccgcgt cgtgaaacgc tggaaatggt ggttcgcgat ctctatccac gcggcggtca
    11221 tgtcgagcag gcgactttct ggacgggttt gcgcccgatg acgccagacg gtacgccggt
    11281 tgtcgggcgt acacgcttta aaaatctgtg gctgaatacc ggtcacggca cgctcggctg
    11341 gacgatggct tgcggttccg gtcagttgtt aagcgatctg ctctctggtc gcacgccagc
    11401 gatcccatat gaggatctaa gcgtagcgcg ctacagccgt ggatttacgc catcacgtcc
    11461 gggccattta catggcgcac acagctaagg aaacgagatg acccgtccga tacaggccag
    11521 cctcgatctg caggcattaa aacagaatct gtccattgtc cgccaggccg cgccgcacgc
    11581 gcgcgtctgg tcggtggtaa aagcgaacgc ttacgggcat ggtattgagc gtatctggag
    11641 cgcgctcggg gccaccgatg gctttgcatt acttaacctg gaagaggcaa taacgttacg
    11701 tgagcgcggc tggaaggggc cgatcctgat gctggaagga tttttccatg ctcaggatct
    11761 ggagatttat gaccagcacc gcctgaccac ctgcgtacac agcaactggc agctcaaagc
    11821 actgcaaaat gcgcggctaa aagcaccgtt ggatatttat cttaaagtga acagtgggat
    11881 gaatcggttg ggcttccagc ccgatcgcgt gcttaccgtc tggcagcagt tgcgggcaat
    11941 ggcgaatgtt ggcgaaatga ccctgatgtc gcattttgct gaagcggaac atcctgatgg
    12001 aatttccggc gcgatggcgc gtattgagca ggcggcggag gggctggagt gtcggcgttc
    12061 gttgtccaat tcggcggcga ctctgtggca cccggaagcg cattttgact gggttcggcc
    12121 tggcattatt ttgtatggcg cttcgccgtc cggtcagtgg cgtgatatcg ccaataccgg
    12181 attacgtccg gtgatgacgc taagcagtga gattattggt gtccagacgc taaaagcggg
    12241 cgagcgtgtg ggctacggcg gtcgctatac tgcgcgcgat gaacagcgaa tcggcattgt
    12301 cgccgcagga tacgccgacg gttatccgcg ccatgcgcct accggtactc ctgttttagt
    12361 ggacggcgtg cgcaccatga cggtggggac cgtctcgatg gatatgctgg cggttgattt
    12421 aacgccttgc ccgcaggcgg ggattggtac gccggttgag ctgtggggca aggagatcaa
    12481 aattgatgat gtcgccgccg ctgccggaac ggtgggctat gagttgatgt gcgcgctggc
    12541 gttacgcgtc ccggttgtga cagtgtaact tgttgtaagc cggatcggag gcagcgtctt
    12601 ctggttgcga aaaaatcatc catccggctg gtcagcaact gtagttgtta atgtgacaga
    12661 gccattgccc atgatagtgt ccattaaaag aatggacact atttccccgg aacctgaact
    12721 cgccgcacag gcgttctaca taaaacgctt acgcttcatt gttgactcgg agtcgactta
    12781 gataaatcac gcttcaccct tgatgcagat tcgggttcat gttcagattg ttgattaaca
    12841 ggctgcgagt cctgttgtct ttccagactc aacaggccca actgttgatc caggagctcc
    12901 aggtcctcct gtaccttcac gcgctccagg agttccgggt catccaggaa ctccagatcc
    12961 ataaggtcct ccagctcctc gagtccttcc aggtcaacct agccttcctt gtgcaaaaag
    13021 tccaaaaggt ccttcaggcc ctgctgtttt ttcaggccct cgacggcttt gagcagcgtc
    13081 ggtaaattgt ctttgtccca cgggttacca ttacagtcaa acaccttcag gctctggggc
    13141 tgcggcacct gtaccgtttt aaacttgttt tctccacagt agtaaattat tagtttgggc
    13201 ggcagccgtg gtgaagagat caaatcgtta ctagaacagt caaatacttt caggcgagtc
    13261 aagttctcca gcatctcatc aggtattgag ttaatattgt tgccggcaca ggatagttta
    13321 tacaggtact ggggcatcga cggcggtaaa aattgcaaga agttttcaga acagttaatc
    13381 actctcaggt tagtcgacag ctcaggcagc cagataatac agttttcata gcacttcagt
    13441 accatcagtt ttgagggctg caccttcggt aacaagatca agacgttatc tgtacagtca
    13501 agcaccagca gccactttgg catcaccttc ggtaacgcgg tcaacacgtt tttactgcag
    13561 taaaattcaa tcagatgtgg aggcaaattc tcagggagtg aagtcaatcc caggttgcta
    13621 agattgagca tactccgtct tgtaatgaga cattccttca ttctgcgcaa tgcttctgcc
    13681 cggttttcaa tccggcttgc tggcaggttt tcttccagga tattttcctt gatttcttcc
    13741 agactttctt tcaggtcttc ttggggaatt tctgactgtt tgttttgctg gttttcttcc
    13801 aggatttcgt acaggttttt ttgcaggttt tcttgcaaac catcctgctg tatagcccat
    13861 tcgcaccatt tggcgaccat catgaactcg ttatgttgct gattcaggcg agtccccaac
    13921 gtgatgatgg tattgttgtc cacctgtatg ttcactttgc ccgcgatact gaggtcaatg
    13981 atctgccagg gggcatcacc ccagctgaat ttttgcgggc tgagatactt catgttctgc
    14041 aaaatttgat tcttgagttc caccgacagg aagagccgcc ctggcgggcg tcgtacgtct
    14101 gggttaacgg aagatggggg aatggcgggg gcgaatgggc agatgtcttt aaagctagcc
    14161 ataatgctac gtttccttga aataaatatc ctctggcagc gccggagacg tgaatgtggt
    14221 caccgcaaag gagccacagg acgtcatttg cgctgatatc actactcatc gttgactttt
    14281 tccctggtca gaaatgccgt gtcgggtctg gtacatgctg tattacggcc taggtagctc
    14341 tgacgtatta aagaaaatac gagagtgaac gacccggaaa taatcgttat taagcaaagg
    14401 aataactata agaaatcgct catgcatccg gtgctacagg cggatgaaac gcctattgtg
    14461 tgggctatgt cagtgcggca ggttgcgtgg ggaatatgtt caggcgatgt caggcagggg
    14521 cccctgatgg ttgaccggta atgcggggct ggtttttatt acctgttaca gtcggaaaaa
    14581 gcccttacgc tgactttctt gagccggtgt tttgagttca ttagccagag cctgcaatgg
    14641 tttgtcatcc ataatttaac cttcttctga tgttgggatg agcatatcaa aatcaggaaa
    14701 tgacaagttt attacaggct caggctgcgt tcagtaaagc atcgcatccg gcgatagatg
    14761 ctgcattaaa tttaagattc agcttcctct tcagccaccc gaacgccaat cttcaacact
    14821 tcattatctt ctttctcggc caccgtccag atcatcccgg caaactctac ctggtcaccg
    14881 acaaccggtg ccgcgcctag caactgctgg acaatttcac ccagcgtttg ctgtttatca
    14941 cgatattctc gcccgtcttc caggccatat atcagcgcaa catcagcata tttggcgctg
    15001 gcttcgagaa tgaagtcacc aaagaagcgt tgatccagcg cgaccggtgg cgactggctg
    15061 aacagtttac cgagcgccgg gagatcgcgt tcccgaccaa ttacacacaa tacatcgcct
    15121 tcgcgcagtc gggtgctgcc ggtaggatga agcaactggt tatcacgaaa cagggctgca
    15181 atacgcgtct cttttggcat atgcaaatcg cgcagtgccg cacctacaca ccatttatca
    15241 gcactcaatt gataaacaaa ctgctcccac ggattttccg gatgaatatc caggccaacg
    15301 cgtgacaccg gacgtccgac gggcggaacc accactttgg cttttttcgc cgcccacgag
    15361 agtgatgttc cctgcaagag cagtgaaacc agaaccacaa agaaggcgac attaaagaac
    15421 agtcgcgcat tctccagccc cgccatcatc gggaacacgg ccaggatgat tggcaccgcg
    15481 ccgcgtaatc ccacccagct gataaacacg cgctcacgca gattgaaacc gcggaaaggg
    15541 agcaatccag caaataccga aaggggacgg gcgaagaata tcatccatgc cgacaaaatg
    15601 agcgccggaa tggcaatagg cagcagatcg cttgggttaa ccaatagccc cagcaccagg
    15661 aacatggcga tttgcgccag ccaggcgagg ccgtcgaaat tttgcaggat gccgtagcgg
    15721 ttgcgaatcg gccgattacc cagcagaaaa ccgcacagat aaacagccag aataccgctg
    15781 ccttccagcg ccgtggttaa agcaaaaatt aaaataccgc cgcttaatgc cagcaatgga
    15841 tataatccgg caggcagggc gatgcgatta atcatttgca gcagtaaata accaccgcca
    15901 agaccgatga caatcccgag gccaaattgc tgcagaatat cgaccacgaa catccaactg
    15961 acactgcttt catgctgctg gatcattgca atcagggtaa tcgtcagaaa gaccgccatt
    16021 ggatcattac tgccggattc aatttccagc gtcgagccaa cacgttcgtt aagcccctta
    16081 ccacccagca gagaaaagac cgctgcagca tcggtggagc cgacgatagc gccgattaat
    16141 aagccttcaa tcaaatcaag attaaacagc cacgccgcca tcatgccggt taaaccagag
    16201 gtgataagta cgcccagcgt cgccagcgac agcgccggtc ctaacgccac acgaaaggaa
    16261 ctggcctgag tgcgcatccc gccgtcgagc aaaataatcg ccagcgccag gttacttacc
    16321 atgtaggcga aggggtaatt atcaaacggg ataccgccga cgccatcgac tcccgccagc
    16381 atgccgatcg ccagaaaaat aaccagaata ggaatgccaa gacgggaaga aaatgaacta
    16441 agtaatatac tgctggtgac aaggatggat cctaagatga aaaggctaat tattgttgtg
    16501 gcatccaacg gtcggttact cctgattacg ctgtctctta tataaaccct accatattag
    16561 cggcagagac agcgttttac ttagccctga agcgtgattt ttttacattt taagaacagg
    16621 atgaccgcta atcgtcaact gagtgccttc ccgggtgtta ttcagaatgg catgtgcgcc
    16681 caacggcagc gtaaccgtgc gttgttcatg accaaaatcg aggccggtaa tgagtggaat
    16741 cgacaggcgg gaacgcaaaa acgcgtacac tgactccagg ttgtaacctg cgtcataatc
    16801 attgggcgtg ctgccgctaa agctaccgag aataatcgcc ttctgacgtg gcaaaattcc
    16861 cgcatgatag agctgcaaca gcatacgttc gacgcggaaa gggtgctcgt taatatcttc
    16921 cagcaccaga ataccgttct caatttttgg catccacggt gtaccaatca gtgaaatcag
    16981 catcgcaaga ttgcctcccc acaacgtgcc ttcggtctga catgtcgggc cttccccttg
    17041 ccattcaatg gtgaaggttt cattgcgtaa cgccagccag aaatggtgct cggtaaaggc
    17101 gttcagttca tccgcaccaa agtttgctac cagcatgggg ccgctaaagg tgatgacatt
    17161 gccttgcgcc agaagaccgc actgaatggc ggtaaaatcg ctatgtccgc aaatgagtaa
    17221 cgggtcatgt tgttggcgag ccaccagtgc ctgccagtca atatctgcca gtaaacgact
    17281 ggcaccgtaa ccgccgcgta cagccagcac gatggtgttg ggggtagtca gtctggcaag
    17341 ggaattgaga tcttccagac gctccgtttc cgtacccgca aaacgctcac aacgacgggc
    17401 aataacctcg acgttattga cttgatgccc cgcgtcggta aggcgctgga taccacgcag
    17461 cgcggcgtgc tgtttaatgc tgtaacccga tggggcaatt aagtgaaaca gagacatggc
    17521 aattccttgc tgacaacaga aacgaaatgt atatcatgcc gcttaggtgt gccgttgtca
    17581 cctcaacggc gattccaggc tataaggata gaagaagtga aattgagatg gtttgccttt
    17641 ttgattgtgt tattagcggg ttgttcatca aagcatgact atacgaaccc gccgtggaac
    17701 gcgaaagttc cggtgcaacg tgcgatgcag tggatgccaa taagccagaa agccggtgca
    17761 gcctggggcg tcgatccaca attgatcacg gcgattatcg ctatcgaatc gggtggtaat
    17821 ccgaacgcgg tgagtaaatc gaatgccatt ggtttgatgc agttaaaagc ttcaacctcc
    17881 ggacgtgatg tttatcgccg tatgggctgg agtggtgagc cgacgaccag cgagctgaaa
    17941 aatccagagc gtaatatttc aatgggggcg gcttacctga atattctgga aaccggcccg
    18001 ctggcaggca ttgaagatcc gaaggtactg caatatgcgc tggtggtgtc atacgctaac
    18061 ggggcaggtg cgctgctacg gactttctcg tcagatcgga aaaaggcgat cagcaaaatc
    18121 aacgatttgg atgctgacga gttcctcgat cacgtagcgc gaaatcatcc tgcgccgcag
    18181 gctccgcgct atatctacaa acttgagcag gcactggacg cgatgtaaat cagtcgcgca
    18241 ctttgtccgc tttttcccgg gcttctcgct cgagagagaa aataatccgc tgtaattgcc
    18301 gctccaccgt cgggctgacg ttaagaaaac ggaagctcag acggggagtg gtgatggttt
    18361 cattcttgcc atcaatcact ttgcgctcgc tggtggagat taactgggcg tcaaagtgaa
    18421 aaacacccca ttgccccatg ttgacttcaa tttgagcgaa gcgcatacct tcttgtaatt
    18481 cggcaggctt tgctgtttcc agtaatgcgc ccatgccgcc taacgacaaa tcatacaagc
    18541 ggaaacgtaa cgtactgtta tccgccagtt tggtctggca aaaataaggc ggatggagtg
    18601 gggcggagat gcggaaatat cggcgtcgtt gtacaaacca taaggtggga ggcggtacgg
    18661 taataaatgc cggaagctgc aagtattcac tctgctgtag ttgttcaaca gtaaactcga
    18721 ctttcgcacc ctgagtttcg gcggtaatgg taatgtgctg tgcctttagc acggcgatgt
    18781 tgtcttcggc ttgactgccg aaatccagca ccagtttatc cggggttatt gccagtattt
    18841 tgctgatcag ctgcccgcca ttccaactga gacgcaaagg aattgcggct ttgtgcaaat
    18901 cgcgtaacac gcccaggacg gctaacggat tttgtttcag gaactgctca tggtaatgac
    18961 tcacgcggaa aaactcctga ctgacaaact gtctttggtt tatcggtcat agttaacaaa
    19021 acttaataca aatgcgtgaa tattttttac atgttgttct taaatcagcc gacaaacgcc
    19081 cagcatcgat cccgcccttg cctatactta gagcgttgac gtaagcattt cttgcgaccg
    19141 attcatcgaa acgagggcct gaacatggga attattgcct ggattatttt tggcctgata
    19201 gccggcatta tcgccaagct aatcatgccg gggcgtgatg gtggtggatt tttcctgacc
    19261 tgtattctcg ggatagtcgg tgcggtggtc ggcggctggc tggcgaccat gtttggcatt
    19321 ggcggctcca tcagcggttt taatttgcac agcttcctag tggcggtggt gggcgctatc
    19381 ctggtactgg gcgtattccg cctcctgcga agagaataag attttcataa ggcggatagc
    19441 gatacagatg ccgctatccg ctttcacatc agaacgtata ctcgacacct gcccagaaat
    19501 tgcgaccttc ttcaataaac ccttcactgt aggcatattg ggtatcaaac aggttattga
    19561 ccgacgcatt gacgctgaag ccatgaccta aggtgtaatc ggctcgaatg tgggtcaccg
    19621 caaaaccggc ggctttttgt gaaccgtcac tgttgctgta gctggaggaa cgcgcctctt
    19681 ccgacagcgt tacgcttaac ggctcccatg gtttgagtgt catccatgcg gtcattgtct
    19741 gcgttggaag atcggttatc ttgccgatgt ctttgcgttt ggcgtcagcg tggatcagag
    19801 cgtagctcaa tcctacatcc agtatattgc tgattttccc cttaataccg gcatccagac
    19861 cgctgtaatc caccgtgccg ctgttctgat tttgaatggt atcggcattg atattgtgcg
    19921 agaggatggc atcactcacc cggttatagt aaacgctgac ctcaaatccc cagtcgcgcg
    19981 tgaaggcacc attccaggtt aaatccaccc cgcgagcgcg ttccggcttg agctgcgggt
    20041 taacaatcgc tatctggtta tacgcaggtt tggatgtggt atagcgttct ttcagcgtcg
    20101 gaaagcgtgt gcggtcatag tacgaaagcg ccagagtgtc ttcattggca aagtggtatt
    20161 tccccatcac ctgccagtta aaagctgact gattgttgtc gtcataatgg gtgatgctgc
    20221 catctttctc gtgttttttc gcttctacgc tatcgcgcca gtcatagctg attcccgcca
    20281 cgacatcgac attatcagca gcagcccatt gatattcact ggcgagcgac caggtacgat
    20341 cttcatagcg atcgtaagcg gcgtgcggcg cacctttttc ccggtgtacg tcatctttcc
    20401 agttaacggc aaacgacagc agatcgtttt cacgcacatc ggctgccagt tgtaatccgg
    20461 caccgtcgct gtaatcggaa taatggctgt agctgccttt tttatttttc aaatcagcca
    20521 gcgagttgta catcatcagc gtattttcaa aggtgtcgcg atacagccga cttttcaggg
    20581 taaaacggtc gtttagttgg gtcgctccct gataataaaa actttctttg tcatactctg
    20641 gccactgcca gtagcgtgat ttttgaccac aatttccgct gtatggcggg ttatcttttt
    20701 caccatcctg cttaatgtaa gtcagcgtgt attcatcgtt ttcgcgcggt gtaaaaccga
    20761 gcttcacaat gccgcgttta tcatcagccg atgaattaat catcttgccg tgtttgcctg
    20821 caatatcatt atttacacca tgcggcaggc cgagaaaatc ctgctttagc tggctaccgc
    20881 tgacttgcaa atacccaaga tcgctgctgg cggcaaatga agcatgcata tcgtaggcat
    20941 tgtcctgact acggctccat ccctggcgat atcccagact tgcttcttca gggtggtgat
    21001 attaatggct ccgcccatct gattaggccc atgaagcagc gacgaatacc ctttggaaac
    21061 ttcgactgcc cccagattgt tggtcagaac ccgcgccaga tcgaggttgc cgtcataggg
    21121 aacataaatg ggcacaccgt cgaaatagac cggcacctgc cgactatcaa agccacgaac
    21181 ttttacctgt tcttcgttgc ggctgcctga cttttgcagc accacgccgg ggacgacact
    21241 taatgcctga gcgacattct gtttatccaa ggctttcatg gtgggttgat cgagaacggt
    21301 ggtcgtcgtc gatgataccg gactggacca tacggtcagc gtgtcgctct cctgagccgc
    21361 tacggcctgc tgggtaaagg caagtgataa tgctgtgcag agataacgtt ttttgagtct
    21421 catagaataa attccgttta acaaagaatt aaatagaggc tgtgggttgc agactgactt
    21481 taatgtcagc tggtgccgcg taataaggcg cggaggtaat aaaaaggcga atgccgcagt
    21541 cgaggtaatt tttgagtgtc gtcagattaa ttccgccggt cagagccagc gtgcagtgag
    21601 gagccagcga cggcgctatt tgcgcaattt cagttgcctg ttgcggacta aatttgtcga
    21661 gctgaagtac gtctggttgt gcgcgtaacg cggcgatagc ctctttcggc gtgtcggctt
    21721 caacaacaat tttcttctcg ggtgcgtggc gacgtaactg agcgattgcg cctgaccagc
    21781 cctgattgtc atgaagaaaa tggcggtggt tggcaaacaa taatatggtt tccgcacatc
    21841 cggcacgatg aattagtcct ccggcagcca gaattgcctg cgaagccagt aagcgggtgc
    21901 ccggaattgc ttttcgggtg caggcgatat tgccgtcagg ataacgttca cgaagcaatg
    21961 ccagcatttg agcgagataa tcagaaacac cgcaactcca ctccagcaca ttctggactg
    22021 ccttccatcc ctgatgaagt gctgcggcat taccctgcgc acggattagc cgttgacccg
    22081 cgttcgcttg tgaaccgtcg ctgaccgcgt catcaatagt taatcccagc gtagttaaca
    22141 tcttacacgc gacagaaacg ccgctgacgc agccgccctg acggtgaaaa aacgctatat
    22201 agccaggctg gtgtccaata tttaatgctc gcgtggtcag gtcacctccc tggatatctt
    22261 ccagcagtaa cgcatcgatt tgtgcctgag aaaggaaaat catcacagtg cctcctgcgg
    22321 tacgctatcc caccagatga gcgcccagtc gcggctggca ggggcgaggg cacgagcatc
    22381 ttgttgttga taccagtgat ataaccgctc acgctcatcg tcgttaagcg cgccaagact
    22441 ccagctgacg ttctgctcaa aacgttccca ggtgctgtta tcctgttggc aattttgtcc
    22501 acggataaaa tcaacgtggg cataaattcc catctgatac aggacgttga gcgcaaagat
    22561 atagttgggt aattcgatca cttcccgacc agccgcccgc tgaatggctg gagagacgaa
    22621 cgaggtgctg accagatgag ttgtatagac gcgaagccgt gcctgattat tcagtttgct
    22681 cattgcatgg cgcatatcag ccaccagagt ggagcgggaa gccacggcaa tatcgcagcg
    22741 tggcaggtca ctccagtcct cttcccaggc gcgctggatc cagtggacat tgtcagcctt
    22801 cagtgcggcg gcgcggcggg cggcaacgtt cagcatcccc tgactgtaat caacgccata
    22861 aaccgtagtg agtttgtcag ccagcgccag cgatacggtg cctggtccgc agcccatatc
    22921 gaataaggtc tgagcgcctt gcaggtcaat tttagcgatc agttgttgca ggtagctgtc
    22981 cgttggcgag gcgcaatttt ccgccatttt ctccgcccgt tgatcccagt gatcgggcgt
    23041 tttttctgtg cggtgagcca gtttgagttg ctgcagataa aggtctgcaa aatcaatatc
    23101 atcaatgagc atgattgttc cttagcggct aattgcagag aggtgatgat gaatttggtc
    23161 ggcagaaacc cgatacaggg ccgccagttt gttcgttgtt aactgttcgg tcggtaagcc
    23221 ttgtgttact cgtccatctg gctctacctg gataatgctg tcggcaatgg cgttggcatg
    23281 gagcggatgg tgagtcgaca ttaacattgt catgccgtta tttttgagct gcgccagcgt
    23341 gtccagaagt tggatctgat gaccgaaatc gaggctggag gcgggctcat ccagtaacaa
    23401 caagcgcggt tgttggacga gtgcacgggc gattaaagcc agctgacgtt caccgccgct
    23461 aagcgtattc cagcgtcgtg tcgccagatg caaaatgccc agcttttcca gttgctccgt
    23521 ggctttcagg cgttcttgct taccgggaac ggaaaaagcg cctatggtgg gggccaggcc
    23581 catcaacacc atatccagca cactgaaagc aaaaataccg tcgtgcgcct gcggcaccca
    23641 ggcaatagcc tgtgcgcgct ggcgatggct gagtgtctgc acagggaggc tatccagtag
    23701 gatctcgcct tttaataacg gtataacgcc aagaatggag cgcatgagtg tggttttgcc
    23761 gcaaccattc gcgccaagca gacaacaaat tgttccttgc gacagggtga aggacacgtc
    23821 gcgtagcact ggatggcggg agtaacccag agacgcatga cgaacggcaa gcaaagtcac
    23881 tggcgacctc cgcgtaacaa caaaagcagg aaaaacggtg cgccaacaaa agcggtcaga
    23941 atgccaagtg ggatttctgt tgttccaatg ctgcgcgcca gcgtatcggt aagcaggagc
    24001 agaatagcgc cagtacacat tgccataggc aacagctgct gatggttatg accggtcaac
    24061 aagcgggcaa tgtgcggaac gaccagaccg atccaaccaa ttatgcccgc tattgctacc
    24121 gtgctggcgg tgattagtgt ggcgcagaca atcaatccaa agcgcagacg cgtcacgttc
    24181 agtcccagcg aacgggcttc gtcgtcggaa agcgtgagta aattcatgcg ccagcggaga
    24241 aaaaacaggg gcaaggagcc tatgagaatg atcggtgcag catagcagag atcgcgtaag
    24301 gtcacggtgg acagtccccc cagcaaccag aaggtaattg acggtaactg ggtgtaagga
    24361 tcggccagcg ttttaattgg cgatatcccc gctccgcaca acgttcctaa cgcaatgcct
    24421 accagcacca gggttaagat cggatcgtgg cgggtcaccc gacgcgtaat caaccagacg
    24481 cccgcgacca ccatcagctc gccgcaaaat gcgtacagct ggatgtaaac aatgggtaat
    24541 ccccacagga ttgccgcgca ggcaccaagt ccggcaccgg cggagacgcc taaaatatcc
    24601 ggtgaaacta agggattacg caacattccc tgataggtgg ttccggctcc tgccagcgct
    24661 gcgcccagta acagcgcagc aagaatacgc ggcacgcgga tttgccagaa gacaatttgt
    24721 tcctgcacag acacattttc ttgacgaaga attagcgcca gcagttgctg catatcgaga
    24781 tgataagccc cacttacggc ggcaaaagtg atactgatag ctgccagcag caaaatacat
    24841 ccgtttacga ttctcattag ctcgccacca atttttgata gtcggcatca ctgagtgaac
    24901 aatgccagaa cagttgggcg taatgctgca tgtcactttt aaactggcga ttgatggcgg
    24961 gatccagcca ggcgtgaagt ctgcgtaatc ccagaagacg gttgattccc ggcggggcat
    25021 cgagccagcc aaagggcaag ccacttaaaa ataagatgcg ttgttccgca acggctttca
    25081 cgccctgcca gagaggatca cgacgaataa aatctgcagt aacggcctcc tgaaccagaa
    25141 taatatccgg ctgccagcgc aggagatttt ccatggaaac ctgtgtcagg ccgtggcgat
    25201 cggctatttg cgcgacgttg tgcaaaccta ataattccgc cgcctcggta tgcaacgaac
    25261 cctgtaagcc cgtttccagc cctcggggac cgcgcgcagc ataaaagcgg aggttagcgg
    25321 cgggtgaggt ggcgaatgcc tgagcttcac caacgaagtg gctggcgaga ttggcttgtt
    25381 ctgcggcgcg gtgctcttcg cctaacgttt tgccaagcgt tgttaactgt tctgctgatt
    25441 gcgccagttt tccgttaagc aataaccagg ggatttgtgt ctgttcgcta acctgccgtg
    25501 cctgggagat caaggtttca tccgtattgc cgcaatcaac gaccaaatcg ggatgtaacg
    25561 ccataagccc ttccagcgat agtgtgctgg cgcgcccggc gagtcgtccc agcctgggta
    25621 actggcgaat gtgctctggc aatggaatta atgcctgacg ggcaaaatca aaagaggaaa
    25681 aaccgaccat tttttctggc gcgacagcca gcaatagcaa atcggcgggc gcgcctgcgc
    25741 tgacgatgcg tgtaatgttt tgggcttcgg gaagagattg cgaagcaaaa cgattaactg
    25801 tttgtgaaaa ggacggtaga gactgcagga gcagggtaga tgcaagtgcc tgcgcaaacg
    25861 tccgtctggt tatgggcatg gtga
//
LOCUS       DAJVQH010000048        25207 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJVQH010000048 DAJVQH010000000
VERSION     DAJVQH010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.048"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..445
                     /locus_tag="OQM87_002045"
     CDS             <1..445
                     /locus_tag="OQM87_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1508187.1"
                     /translation="FDYLRSPGSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKP
                     LDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQ
                     YTLGLIYRNGTGINVNHYESQKWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(455..1882)
                     /locus_tag="OQM87_002046"
     CDS             complement(455..1882)
                     /locus_tag="OQM87_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCS1508188.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1879..2586)
                     /locus_tag="OQM87_002047"
     CDS             complement(1879..2586)
                     /locus_tag="OQM87_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1508189.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2744..3727
                     /locus_tag="OQM87_002048"
     CDS             2744..3727
                     /locus_tag="OQM87_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1508190.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3797..4279)
                     /locus_tag="OQM87_002049"
     CDS             complement(3797..4279)
                     /locus_tag="OQM87_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCS1508191.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4514..7096
                     /gene="leuS"
                     /locus_tag="OQM87_002050"
     CDS             4514..7096
                     /gene="leuS"
                     /locus_tag="OQM87_002050"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCS1508192.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7111..7692
                     /gene="lptE"
                     /locus_tag="OQM87_002051"
     CDS             7111..7692
                     /gene="lptE"
                     /locus_tag="OQM87_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCS1508193.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7692..8723
                     /gene="holA"
                     /locus_tag="OQM87_002052"
     CDS             7692..8723
                     /gene="holA"
                     /locus_tag="OQM87_002052"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCS1508194.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRTLFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8725..9366
                     /gene="nadD"
                     /locus_tag="OQM87_002053"
     CDS             8725..9366
                     /gene="nadD"
                     /locus_tag="OQM87_002053"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCS1508195.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9390..10001
                     /locus_tag="OQM87_002054"
     CDS             9390..10001
                     /locus_tag="OQM87_002054"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCS1508196.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10261..10578
                     /gene="rsfS"
                     /locus_tag="OQM87_002055"
                     /gene_synonym="rsfA"
     CDS             10261..10578
                     /gene="rsfS"
                     /locus_tag="OQM87_002055"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCS1508197.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10582..11049
                     /gene="rlmH"
                     /locus_tag="OQM87_002056"
     CDS             10582..11049
                     /gene="rlmH"
                     /locus_tag="OQM87_002056"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCS1508198.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11080..12981
                     /gene="mrdA"
                     /locus_tag="OQM87_002057"
     CDS             11080..12981
                     /gene="mrdA"
                     /locus_tag="OQM87_002057"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCS1508199.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12984..14096
                     /gene="mrdB"
                     /locus_tag="OQM87_002058"
                     /gene_synonym="rodA"
     CDS             12984..14096
                     /gene="mrdB"
                     /locus_tag="OQM87_002058"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCS1508200.1"
                     /translation="MTANPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14107..15195
                     /gene="rlpA"
                     /locus_tag="OQM87_002059"
     CDS             14107..15195
                     /gene="rlpA"
                     /locus_tag="OQM87_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCS1508201.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15334..16545
                     /gene="dacA"
                     /locus_tag="OQM87_002060"
     CDS             15334..16545
                     /gene="dacA"
                     /locus_tag="OQM87_002060"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCS1508202.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16656..16919
                     /locus_tag="OQM87_002061"
     CDS             16656..16919
                     /locus_tag="OQM87_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCS1508203.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17020..17661
                     /gene="lipB"
                     /locus_tag="OQM87_002062"
     CDS             17020..17661
                     /gene="lipB"
                     /locus_tag="OQM87_002062"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCS1508204.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17920..18873
                     /locus_tag="OQM87_002063"
     CDS             17920..18873
                     /locus_tag="OQM87_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1508205.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19082..20047
                     /gene="lipA"
                     /locus_tag="OQM87_002064"
     CDS             19082..20047
                     /gene="lipA"
                     /locus_tag="OQM87_002064"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCS1508206.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20148..20351)
                     /gene="tatE"
                     /locus_tag="OQM87_002065"
     CDS             complement(20148..20351)
                     /gene="tatE"
                     /locus_tag="OQM87_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCS1508207.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20480..21268)
                     /locus_tag="OQM87_002066"
     CDS             complement(20480..21268)
                     /locus_tag="OQM87_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCS1508208.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21361..21744
                     /gene="crcB"
                     /locus_tag="OQM87_002067"
     CDS             21361..21744
                     /gene="crcB"
                     /locus_tag="OQM87_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCS1508209.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21798..22007)
                     /gene="cspE"
                     /locus_tag="OQM87_002068"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21798..22007)
                     /gene="cspE"
                     /locus_tag="OQM87_002068"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1508210.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22182..22742)
                     /gene="pagP"
                     /locus_tag="OQM87_002069"
     CDS             complement(22182..22742)
                     /gene="pagP"
                     /locus_tag="OQM87_002069"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCS1508211.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23331..24716
                     /gene="dcuC"
                     /locus_tag="OQM87_002070"
     CDS             23331..24716
                     /gene="dcuC"
                     /locus_tag="OQM87_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1508212.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24757..25098)
                     /locus_tag="OQM87_002071"
                     /pseudo
     CDS             complement(24757..25098)
                     /locus_tag="OQM87_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         6417 a         6274 c         6215 g         6301 t
ORIGIN      
        1 ctttgattat ttaagatcac caggcagtct accgttaacc acggcagcca cggagctttc
       61 cgcgattcat ggtcactcaa catcgcaata tcgccttggc gaattttatc ttcatggtag
      121 cgacggtaaa ccactggatt atacacaggc gagatactgg tatgagcaat cagcagaaca
      181 ggaaaatccg cgcgcacaaa gcaaactggg gtggatctac ctgaaaggcc tgggagtcaa
      241 acccgatacc cgtaaagcaa ttctctggta taaggaagca gctgaacaag ggtatgctca
      301 tgctcaatat actttgggtt tgatctacag aaatggcact ggtattaatg ttaaccatta
      361 tgaatctcaa aaatggttaa aactggccgc caaacaacat tacaaaaatg cggaaagatt
      421 acttgccgga cttcccgcac attaacgtcg actgtcaccc gaagagatgg agtattttcc
      481 ctatcaaacc ggcaaagata aaaatgtaga aaataatttt gatgacgccc agacagccca
      541 gcccgccatc gtcaacgggc attggctgcg gttgcggctc cggctcgacg gtttcctgac
      601 cggtaaaatt cgccgcctga attaataatc gacgaataat taatgcttcc agtggacaac
      661 gatcttctgc atcaaggcgg ctcacaatcg gcattaatga ttcttctgtc ggttggctgg
      721 cctgcactaa ttgtttggca actaaatata atggccataa gggatgttct gtatcacgcg
      781 tgagtgagat ccgcggctta cgtcctttgt gcaaaaccat ctcgaccagt gaacgcccca
      841 gcgcagagag tttttcatcg ctgagtattt cgtataaatg aaaacgcgta cgggcgctgt
      901 cgccggggac ataaagtagc ggttttccct gatcgttaac cagatcacgc gcttcaatca
      961 tcatcaccac taatgggtaa taatcgaccc gatgctgacg gcaccattgc aacattaaag
     1021 catccgcctg ggtggaggga tactcgcgcc agtagtaaca aagttctgcc agcaggcttt
     1081 cgccttctcc acaatagacg cgttgtgcgt attcataata acgcgcatct tcattatcag
     1141 gttcagcttc ccgccacgac cgggcgtaat ccaggagttc ggggattccc aggcggcttg
     1201 cgctatacca gcggagtaaa tttctgtgca gttttgcatc atcggggata atcactgcgc
     1261 gatgttgcgc taaatacaat gtcagccatt ctggatgcga tgaccaaatc cgagcgaaca
     1321 tttggtaaaa atcgatggtg tcattttgca cggcaacggg cagatgcaga atggtctgga
     1381 agttaaaaac attccccttc gcaatggcat ataaaaagga ttccagttct tcttcgtcta
     1441 tttccccgac gttttcctcc tgcaaccacc gcaatctttc tgctaataac accacgcact
     1501 cgaaggataa atgggcagtg ttcatggcga ttgtgcacag cgaccagcgt aattcatcaa
     1561 tctcttccat cgagcaataa tttaattgct gaatgaatcg ctgccaggcg gagggcagaa
     1621 aacgttcgct atcagaggca agtaacgcgc gaaaggcgag cagaatttca tgttctgcta
     1681 cctcatattc ttctggttgc caaacagatt tagcaggcga ctgcgcaatc cgtagcgcct
     1741 cctcatacgc ctgacgaagt tgtttaaaac cctgcggatc ggtttctgga tgaaaggacg
     1801 gtaacagcgc cagataagcg cggcggataa tatcgacgtc agtcgtttcc tctatatcga
     1861 ggatcttcca gcaatttttc ataagtcgct catatcctta agcgtttccg ggcatgtggg
     1921 gtagtcagcc agtggttgcc aggacaaggt agggtaatac gcatcatcac tggcaaataa
     1981 ttgataaaac atatcattgc cggaatcgtc gtattcttta cgcgctaaga attcgcgtaa
     2041 taaaggcaga tgatcatctt cgggagactg ccaatattgc cgaccaaagg tataaccggc
     2101 aaagtattgc cgccagttac tataataacg tagagcccgt agatgaatac gcgacaggat
     2161 ccatagactt tcttcttcac tcaaccagtt attaagtacg cccattcgac ttaaaaaacc
     2221 catccggaca taatcccatg ctttaattcc tccctcacca cagcattcgg ctgtgctggc
     2281 gacaaattgc gcataagcct gaccttgctc atttaattca gcaagatagt cacgccattc
     2341 acatggagaa taacgaaacc agcggtgata aaaccctgcc aaccgagcgg catgaccgtt
     2401 ctcgatcatt cggtgaattg tgcgcagcag gtcatcacgg tcatcaattc cccatgagga
     2461 agaaaggtca aaaaagcatg tacctaaata cattttgggg gatgaatgcg aaggtaaaca
     2521 gcattcattg cgtatttcca tcggagtgga aagtgcaaac aatatttttt gcgaccccat
     2581 gtccatataa tttatcctta aacattcatt taatgaatgc aaactacatt tcgagactca
     2641 gttatttata cttttctgac gcaacataat acgcataaaa taaggattac gcaaaaatgg
     2701 aacacataaa aatataatat attttatcta caaccataag gaaatgataa taatttttac
     2761 gtcaagttgc tgcgataatt tatcaataga tgagattatc gaacgtgctg aaaaaggcga
     2821 ttgcgaggct caatatattg ttgggtttta ttataatcgc gatagcgcaa ttgactctcc
     2881 ggacgacgaa aaggcctttt actggctgaa gctggccgct gagcaaggtc attgtgaagc
     2941 acagtattcc ttagggcaaa agtataccga ggataaaagc cgtcataaag ataatgagca
     3001 agccatcttc tggctgaaaa aagctgccct acaaggccat actttcgctt ccaacgccct
     3061 tggctggata ctggatcgtg gagaagaccc caactataaa gaagcggtta cctggtatca
     3121 gatagccgcg gagagcggaa tgtcttatgc gcaaaataat cttggatgga tgtacagaaa
     3181 tggcaacgga gtcgcacaag actatacgct ggcatttttc tggtacaaac aagctgcatt
     3241 acaaggccat agttacgcac aagacaatct ggccgatctt tataaagacg gagaaggcgt
     3301 tgctcaaaac aagacactcg ccgcattctg gtatttgaaa agcgcacagc agggtaatcg
     3361 gcacgcccag tttcaaattg catgggatta taacgctggc gaaggggtgg accaggacta
     3421 taagcaagcg atgtactggt atctgaaggc tgccgctcag gaaagcgtcg gcgcttacgt
     3481 caacatcggt tatatgtata aacacggaca aggcgttgag aaagattatc aggctgcctt
     3541 tgaatggttt acgaaagccg ctgaatgcaa tgacgccact gcctggtata acctggccat
     3601 tatgtatcac tacggagaag gaagacctgt cgatctccga caggctctcg acctgtatcg
     3661 taaagttcag tcatccggaa ccagagatgt cagtcaggaa attcgtgaga ctgaagcttt
     3721 actgtagaaa taaaaacaag ggccggggaa tttcattctg cccatattct ctttattgct
     3781 atgcgaaatc agactattac ggctcaaacg ggcgtctctg gaactggtca taaccacatt
     3841 tcgggcatag cgtcagcact tccggtgtgt agatcgggag atggaagtga catttctcgc
     3901 agaccagatt tcccagcccg accacttctc cgctgtgata aaccccatga tgattgaggt
     3961 cctggaaaac ttcgcgccat tcaagctgcg ttttatcggt gatgtctgcc agctcctgcc
     4021 acaagctttc tttaatcacc cgcataaaga cgctgtcaga ttcttctttc aggctctctt
     4081 catagctcat ggcgaactct tccaggtcac gtctgacagc tcgcgtcagc tcatcgatct
     4141 cggttcgcgt taactcccct gtttttatta cgcgctcgcg cgcctgttcc accagtgcgt
     4201 cgatatcacg ttcgccattg cgcaggcgtt cgttcagtga cgcgaccagt tcacggtaat
     4261 attgagcaac cttgttcatc gtttcatctc ccgggtcgtt aactgactat aataatagac
     4321 gttctttgtt ccttgctttg ctaatacgcc cacaaactct gtaaattaat gtacgaataa
     4381 tccgggcatc atttcaacgt cgagtgcgcg aaaggctgtt ttgacgcgga cgtttgggct
     4441 atgctatgcg gatctgaaaa accacctcaa cgctacattt gtagccgtat tgaaaacagg
     4501 accactggct gccatgcaag agcaataccg cccggaagag atagaatcca aagtacagct
     4561 tcattgggat gagaagcgca catttgaagt aaccgaagac gagagcaaag agaagtatta
     4621 ctgcctgtct atgcttccct atccttctgg tcgactacac atgggccacg tacgtaacta
     4681 caccatcggt gacgtgatcg cccgttacca gcgcatgctg ggcaaaaacg ttctgcagcc
     4741 gatcggctgg gacgcgtttg gtctgcctgc ggaaggcgcg gcggtgaaaa acaacaccgc
     4801 tccggcaccg tggacgtacg acaacatcgc gtatatgaaa aaccagctca aaatgctggg
     4861 ctttggttat gactggagcc gcgagctggc aacctgtacg ccggaatact accgttggga
     4921 acagaaattc ttcaccgagc tgtataaaaa aggcctggta tataaaaaga cttctgcggt
     4981 caactggtgc ccgaacgacc agaccgtact ggcgaacgaa caagttatcg acggctgctg
     5041 ctggcgctgc gataccaaag ttgaacgtaa agagatcccg cagtggttta tcaaaatcac
     5101 tgcttacgct gacgagctgc tcaacgatct ggataaactg gatcactggc cagacaccgt
     5161 taaaaccatg cagcgtaact ggatcggtcg ttccgaaggc gtggagatca ccttcaacgt
     5221 taacgactat gacaacacgc tgaccgttta cactacccgc ccggacacct ttatgggttg
     5281 tacctacctg gcggtagctg cgggtcatcc gctggcgcag aaagcggcgg aaaataatcc
     5341 tgaactggcg gcctttattg acgaatgccg taacaccaaa gttgccgaag ctgaaatggc
     5401 gacgctggag aaaaaaggcg tcgatactgg ctttaaagct gttcacccat taacgggcga
     5461 agaaattccc gtttgggcag caaacttcgt attgatggag tacggcacgg gcgcagttat
     5521 ggcggttccg ggtcacgacc agcgcgacta cgagtttgcc tctaaatacg gcctgaacat
     5581 caaaccggtt atcctggcgg ctgacggctc tgagccagat ctttctcagc aagccctgac
     5641 tgaaaaaggc gtgctgttca actctggcga gttcaacggt cttgaccatg aagcggcctt
     5701 caacgccatc gccgataaac tgactgcgat gggcgttggc gagcgtaaag tgaactaccg
     5761 cctgcgcgac tggggtgttt ctcgtcagcg ttactggggc gcgccgattc cgatggtgac
     5821 gctggaagac ggtaccgtaa tgccaacccc ggacgaccag ctgccggtga tcctgccgga
     5881 agatgtggtg atggacggca ttaccagccc gattaaagca gatccggagt gggcaaaaac
     5941 taccgttaac ggtatgccag cgctgcgtga aaccgacact ttcgacacct ttatggagtc
     6001 ctcctggtac tatgcgcgct acacttgccc agagtacaaa gaaggtatgc tggattccga
     6061 agcggctaac tactggctgc cggtggatat ctacattggt ggtatcgaac acgccattat
     6121 gcacctgctc tacttccgct tcttccacaa actgatgcgt gatgcaggca tggtgaactc
     6181 tgacgaacca gcgaaacagt tgctgtgtca gggtatggtg ctggcagatg ccttctacta
     6241 tgttggcgaa aacggcgaac gtaactgggt ttccccggtt gatgctatcg ttgaacgtga
     6301 cgagaaaggc cgtatcgtga aagcgaaaga tgcggcaggc catgaactgg tttataccgg
     6361 catgagcaaa atgtccaagt cgaagaacaa cggtatcgac ccgcaggtga tggttgaacg
     6421 ttacggcgcg gacaccgttc gtctgtttat gatgtttgct tctccggctg atatgactct
     6481 cgaatggcag gaatccggtg tggaaggggc taaccgcttc ctgaaacgtg tctggaaact
     6541 ggtgtacgag cacacagcaa aaggtgatgt tgcggcactg aacgttgatg cgctgactga
     6601 agatcagaaa gcgctgcgtc gcgatgtgca taaaactatc gctaaagtga ccgatgatat
     6661 cggccgtcgt cagaccttca acaccgcaat tgcggcgatt atggagctga tgaacaaact
     6721 ggcgaaagca ccgaccgatg gcgagcagga tcgcgctctg atgcaggaag cactgctggc
     6781 cgttgtccgt atgctcaacc cgttcacccc gcacatctgc ttcacgctgt ggcaggaact
     6841 caaaggcgaa ggcgatatcg acaacgcgcc gtggccggtt gctgacgaaa aagcgatggt
     6901 ggaagactcc acgctggtcg tggtgcaggt taacggtaaa gtccgtgcca aaatcaccgt
     6961 tccggtggac gcaacggaag aacaggttcg cgaacgtgct ggccaggaac atctggtagc
     7021 aaaatatctt gatggcgtta ctgtacgtaa agtgatttac gtaccaggta aactcctcaa
     7081 tctggtcgtt ggctaagcgc gggaggaagc gtgcgatatc tggcaacatt gttgttatct
     7141 ctggcggtgt taatcaccgc cgggtgtggc tggcatctgc gtgacaccac gcaggttcct
     7201 tccactatga aggtcatgat cctagactca ggcgatccga acgggccatt aagccgtgcg
     7261 gtgcgtaacc agttacgtct gaatggtgtc gagttgcttg ataaagaaac cacgcgtaag
     7321 gacgttccat ccctgcgttt gggtaaagtg agcatcgcga aagataccgc atcggtattc
     7381 cgtaacggtc aaacagcaga gtatcagatg atcatgacgg tgaatgcgac cgtgttgatc
     7441 cccggccgtg atatctaccc gattagcgcc aaagtcttcc gttcattctt cgataacccg
     7501 caaatggcgt tagcgaaaga taacgaacaa gacatgatcg taaaagagat gtacgaccgt
     7561 gctgccgaac agctgattcg taagctgcca agcatccgtg ctgcggatat tcgttccgac
     7621 gaagaacaga cgtcgaccac aacggatact ccggcaacgc ctgcacgcgt ctccaccatg
     7681 ctgggtaact gatgattcgg ttgtacccgg aacaactccg cgcgcagctc aatgaagggc
     7741 tgcgcgcggc gtatctttta cttggtaacg atcctctgtt attgcaggaa agccaggacg
     7801 ctgttcgcca ggtagctgcg gcacaaggat tcgaagaaca ccacactttt tccattgatc
     7861 ccaacactga ctggaatgcg atcttttcgt tatgccaggc tatgagtctg tttgccagtc
     7921 gacaaacgct attgctgttg ttaccagaaa acggaccgaa tgcggcgatc aatgaacaac
     7981 ttctcacact caccggactt ctgcatgacg acctgctgtt gatcgtccgc ggtaataaat
     8041 taagcaaagc gcaagaaaat gccgcctggt ttactgctct tgcgaatcgc agcgtgcagg
     8101 tgacctgtca gacaccggag caggctcagc ttccccgctg ggttgctgtg cgcgcaaaac
     8161 agctcaactt agaactggat gacgcggcga atcaggtgct ctgctactgt tatgaaggta
     8221 acctgctggc gctggctcag gcactggagc gtttatcgct gctctggcca gacggcaaac
     8281 tgacattacc gcgcgttgaa caggcggtga atgatgccgc gcatttcacc ccttttcatt
     8341 gggttgatgc tttgttgatg ggaaaaagta agcgcgcgtt gcatattctt cagcaactgc
     8401 gtctggaagg cagcgagccg gttattttgt tgcgcacatt acaacgtgaa ctgttgttac
     8461 tggttaacct gaaacgccag tctgcccata cgccactgcg tacgttgttt gataagcatc
     8521 gggtatggca gaaccgccgg ggcatgatgg gcgaggcgtt aaatcgctta agtcagccgc
     8581 agttacgtca ggccgtgcaa ctcctgacac gaacggaact caccctcaaa caagattacg
     8641 gtcagtcagt gtgggcagag ctggaagggt tatctcttct gttgtgccat aaacccctgg
     8701 cggacgtatt tatcgacggt tgatatgaaa tctttacagg ctctgtttgg cggcaccttt
     8761 gatccggtgc actatggtca tctaaaaccc gtggaaacgc tggcgaattt gattggtctg
     8821 acgcgggtca caatcatccc taataatgtt cctccgcatc gtccccagcc ggaagcgaac
     8881 agcgtgcagc gtaaacacat gcttgaactg gcgattgccg acaagccatt atttactctt
     8941 gatgaacgcg agctaaagcg caatgccccc tcttacactg cacaaacact gaaagagtgg
     9001 cggcaggaac aaggaccgga cgtgccgctg gcgtttatta ttggtcagga ttcattgctg
     9061 acctttccga cctggtacga atacgaaacg atactcgaca atgcacattt gatcgtctgt
     9121 cggcgtccag gttacccact tgaaatggcg caaccgcaat accagcaatg gctggaagat
     9181 catttgacac ataacccgga agatcttcac cttcagcctg ccggtaaaat ttatctggct
     9241 gaaacgccgt ggtttaacat ctcggcgacc atcatccgcg aacgtttgca aaacggtgaa
     9301 tcgtgtgagg atttattgcc ggaaccggta ttgacttaca ttaaccaaca aggcttgtat
     9361 cgctgatacc tgtcgttacg gagggcatga tgcgactgtg gttaattcgt catggcgaaa
     9421 cacaagcgaa tgtcgatggt ctgtacagcg gtcatgcgcc cacacccctg accgcacgcg
     9481 gtattgagca agcgcaaaat ctgcatacac tgctagatga tgtttccttt gacctggttt
     9541 tatgcagtga actggaacgg gcacagcata ccgcgcgact ggttctcagt gaccgccagc
     9601 tccccgttca tatcatccct gaactcaacg aaatgttttt tggcgactgg gagatgcgac
     9661 atcatcgcga cctcatgcaa gaagatgccg aaaactatag cgcgtggtgc aatgactggc
     9721 agcatgctat ccccacgaac ggtgaaggat ttcaggcatt ttcgcaacgt gtggaacgct
     9781 ttatcgcaag gcttagtgaa tatcagcact atcagaatat tttaatcgtc agccatcagg
     9841 gcgtactgag tctgttaatt gctcgtttaa ttggcatgcc tgccgagtct atgtggcatt
     9901 ttcgcgttga ccaggggtgt tggagcgcca ttgatatcaa ccaaaaattc gcgacgctac
     9961 gcgtcctcaa tagccgtgcc atcggggtcg agaatgcatg acttttctgt ttttttacgg
    10021 gtaagccgca acgaccattg acagccccgg gcaggctgat attctccgca gccagacttt
    10081 ttccgccaga cacgactttg tagaaattgt tttacaaaaa tggcgatgca atctgcggcg
    10141 cggggtggga tgatagccca ctttcgaaag ccgattcggc gacaattgtc ccgaaatcgc
    10201 ctctggttca ggtatactga cagaccattt ttatctattt gattcaccca gggggaaaac
    10261 ttgcagggta aagcactcca ggattttgtt atcgacaaaa ttgatgacct caaaggtcag
    10321 gacatcatcg ccttagacgt tcaaggcaaa tccagcatca ccgactgcat gatcatctgt
    10381 acgggtacgt ccagccgtca tgttatgtcc attgctgacc acgttgtgca ggagtctcgc
    10441 gcagcgggcc tgttaccgct cggcgtagaa ggtgaaaaca gcgccgactg gattgtcgtg
    10501 gatttgggcg atgtgattgt ccatgtcatg caggaagaga gccgtcgcct gtatgaactg
    10561 gaaaaactct ggagttaatg cgtgaagctg caacttgtcg ccgtgggaac gaaaatgccg
    10621 gactgggtac aaaccggttt taccgagtac ctgcgtcgtt ttccgaaaga tatgcccttc
    10681 gagctgattg aaattccggc cggcaaacgc ggcaagaatg cggacatcaa gcgcatactc
    10741 gacaaagagg gtgagcagat gttggcggcc gcaggcaaaa accgcattgt catcctcgat
    10801 attccaggca agccctggga tacgccgcag ttagccgctg agctggaacg ctggaagctg
    10861 gatggtcgcg acgtaagtct actgattggc gggcctgaag ggttgtcgcc tgcctgtaaa
    10921 gcggcggctg agcagagctg gtcgctgtcg gcgcttaccc tcccccatcc gctggttcgc
    10981 gtgctggtcg cagagagtct gtaccgggcg tggagcatca ccaccaacca tccttatcac
    11041 cgtgagtgat aagggagctt tgagtagaaa acgcagcgga tgaaactaca gaactctttt
    11101 cgcgactata cggcagagtc cgcgctgttt gtgcgccggg cgctggtcgc ctttttgggg
    11161 attttgctgc tgaccggcgt gcttatcgcc aacctgtata atctgcaaat tgttcgcttt
    11221 accgactacc agacccgctc taatgaaaac cgcattaagc tggtgcctat cgcgcccagc
    11281 cgcggcatta tctacgaccg taacggtatc cctctggccc tcaaccgcac tatctaccag
    11341 atagaaatga tgccggagaa agtcgataac gtgcagcaaa cgctggacgc tttgcgcagc
    11401 gtggtagatc tgaccgatga cgatattgct gcattccgaa aagagcgcgc acgttcacac
    11461 cgtttcacct ctattccggt gaaaactaac ctgaccgaag tacaagtagc tcgctttgcc
    11521 gtcaatcagt accgttttcc gggtgtcgaa gttaaaggct ataaacgtcg ttactatcct
    11581 tacggttcgg cgttgaccca cgtcatcgga tatgtgtcga aaatcaacga taaagacgtc
    11641 gaacgcctga ataatgacgg caaactggcc aactatgcgg caacgcatga tattggtaag
    11701 ctgggcattg agcgttacta tgaagacgtg ctgcacggtc agaccggtta tgaagaggtt
    11761 gaagttaaca accgtgggcg tgttatccgc cagttaaaag aagtaccacc gcaagccgga
    11821 cacgatattt acctgacgct ggatctcaaa ctccagcaat atattgaaac gctgctggcg
    11881 ggtagccgcg cagctgtggt tgttaccgat ccgcgtacag gtggggtgct ggcgctggtt
    11941 tccacgccta gttatgaccc aaacctgttt gttgacggta tctccagcaa agattattcc
    12001 gccttgctga acgacccgaa tacaccgctg gtgaaccgcg ccacacaggg ggtttatcct
    12061 cccgcgtcta cagttaaacc ctatgtggcg gtttcggcat taagcgccgg ggtgatcacg
    12121 cgcaatacga cgctgtttga cccaggctgg tggcaactac cagggtcgga aaaacgttat
    12181 cgtgactgga aaaaatgggg ccacgggcgt ctgaatgtca caagatcgct ggaagaatct
    12241 gcggatacct tcttctatca ggtggcctac gatatgggga tcgatcgcct ctccgaatgg
    12301 atgggtaaat tcggttatgg tcattacacc ggtatcgacc tggcggaaga acgttccggc
    12361 aacatgccta cccgcgaatg gaaacagaaa cgctttaaaa aaccgtggta tcagggtgac
    12421 accattccgg ttggtatcgg ccagggttac tggacagcga ccccaatcca gatgagtaag
    12481 gcactgatga tcctgattaa tgacggtatc gtgaaggttc cgcatttgct gatgagcacc
    12541 gccgaagacg gcaaacaggt gccatgggta caaccgcatg aaccgcccgt cggcgatatt
    12601 cattccggtt actgggagct ggcgaaagac ggtatgtacg gtgttgctaa ccgccctaat
    12661 ggtacggcgc ataaatactt tgccagcgca ccgtacaaaa ttgcggcgaa atctggtacc
    12721 gctcaggtct tcggtctgaa agcgaacgaa acctataatg cgcacaaaat tgccgagcgt
    12781 ttacgtgacc acaaactgat gaccgccttt gcgccataca acaatccgca agtggctgtc
    12841 gccatgattc tggagaacgg cggcgcgggt ccggcggttg gtacgctgat gcgccagatc
    12901 ctcgaccaca ttatgctggg tgataacaac accgatctgc ctgcggaaaa tccagcggtt
    12961 gccgcagcgg aggaccatta atcatgacgg ctaatccgaa taaaaaaaca ttctgggata
    13021 aagtccatct cgatcccaca atgctgctga ttttactggc attgctggtt tacagcgccc
    13081 tggttatctg gagcgccagc ggtcaggata ttggcatgat ggagcgtaaa atcggccaaa
    13141 tcgcgatggg tctggtcatc atggtggtga tggcgcaaat tcctccacgc gtttatgaag
    13201 gctgggcccc ctatctctat atcatctgta ttattttgct ggtggcggta gatgctttcg
    13261 gagccatctc taaaggcgct caacgctggc tggacctcgg tattgttcgt tttcagccgt
    13321 cggaaattgc caaaatagcc gtaccactga tggttgcgcg ctttatcaac cgcgacgttt
    13381 gcccgccatc gttgaagaac actggcatcg cgctggtgct gatatttatg cccacgctgc
    13441 tggtggctgc acagcctgac ctgggaacat caatcctcgt tgcgctttcc ggtctgtttg
    13501 tactgttcct ctctggcctt agctggcgtc tgattggcgt cgcagtagtg ctggtagcgg
    13561 cgttcattcc gattctatgg ttcttcctga tgcatgatta ccagcgccag cgcgtaatga
    13621 tgctcctaga cccggaatca gacccactcg gcgcgggcta tcacattatt cagtctaaaa
    13681 ttgctattgg ctccggcgga ttacgcggca aaggctggct gcacggcact cagtcacagc
    13741 ttgaatttct ccccgaacgc catactgact ttatcttcgc ggtactggcg gaagagctgg
    13801 gattagtggg cattctgatt ctgctcgctc tctacattct gctgatcatg cgcgggctgt
    13861 ggatagccgc cagagcgcaa accacctttg gtcgcgtcat ggctggtggc ttaatgctga
    13921 tattattcgt ttatgtcttc gtaaatattg gtatggtaag cggtattctg ccggttgtag
    13981 gggttccgct cccactggtc agttatggag gatcggcgct aattgtgctg atggctgggt
    14041 tcgggattgt aatgtcaatc cacacccaca ggaaaatgtt gtcgaaaagc gtgtaagagg
    14101 tgcgcaatgc gtaagcagtg gctcgggatc tgcatcgcgg caggaatgct cgcggcatgt
    14161 acaagcgatg atggtcagca acagacggta agtgtaccgc agcctgcggt atgtaacggc
    14221 cccatagttg aaattagcgg ggcggacccg cgtttcgaac cactgaacgc gacggcaaat
    14281 caggattacc agcgcgacgg taaaagctac aaaatcgtgc aggatccgtc tcgatttagc
    14341 caggcgggac tggcggcaat ctatgatgcc gaaccaggca gtaacctgac ggcctctggc
    14401 gaagctttcg atccgacaaa gctgacggcg gcccatccaa cgcttccgat ccccagctac
    14461 gccagaatca ctaacctggc taacgggcga atgatcgtgg tgcgcattaa tgatcgcggc
    14521 ccttacggca acgaccgcgt tatttcgctt tctcgcgcgg cagctgaccg tcttaacacg
    14581 tcaaacaaca ccaaagttcg tatcgatccg attattgtcg cccaggatgg ttcgctttct
    14641 ggtcctggta tggcttgtac cacagtcgcc aaacagactt atgccctgcc tgcacctccc
    14701 gatttaagcg gtggcgcggg aacaagttca gtgtctggcc cgcagggtga cattcttccg
    14761 gtcagtaatt cgacgctaaa aagcgaagat ccgaccggcg cgccggtaac cagtagcggt
    14821 ttcctcggcg ctcaaacgac cttagcgcct ggtgtactgg aaggcagcga accgacgcct
    14881 gcgccacagc ccgttgttac agcttcgtcg acaacgcctg caacctcgcc tgcaatggtg
    14941 acaccgcaag ccgcctcgca aagcgccagc ggcaacttta tggtgcaagt cggggccgta
    15001 agcgatcagg ctcgtgcgca acagtaccaa cagcaactgg gacagaagtt cggcgtcccc
    15061 ggtcgcgtaa ctcaaaatgg cgcggtctgg cggatccagc ttggcccatt cgccagcaaa
    15121 gccgaagcca gtaccttgca gcaacgtttg caaaccgaag cccaattaca gtcatttatt
    15181 actaccgcgc agtagcgtaa agcaggcatc tgatgtgtca atttctgtaa atgacgttaa
    15241 caaagtcacg cggaaagtca gatgcctgcc ggtagtgcat ttgctatagt agggcacttt
    15301 tttaattcca tcacggatgt cgtagttcag accatgaata ccattttttc cgctcgtatc
    15361 atgaagcgcc tggcgctcac cacggctctt tgcacagcct ttatctctgc tgcacatgcc
    15421 gatgacctga atatcaaaac tatgatcccg ggtgtaccgc agatcgatgc ggagtcctac
    15481 atcctgattg actataactc cggcaaagtg ctcgccgaac agaacgcaga tgtccgccgc
    15541 gatcctgcca gcctgaccaa aatgatgacc agttacgtta tcggccaggc aatgaaagcc
    15601 ggtaaattta aagaaactga tttagtcact atcggcaacg acgcatgggc caccggtaac
    15661 ccggtgttta aaggttcttc gctgatgttc ctcaaaccgg gcatgcaggt tccggtttct
    15721 cagctgatcc gcggtattaa cctgcaatcg ggtaacgatg cttgtgtcgc catggccgat
    15781 tttgccgctg gtagccagga cgcttttgtt ggcttgatga acagctacgt taacgcactg
    15841 ggcctgaaaa atacccactt ccagacggta catggtctgg atgctgatgg tcagtacagc
    15901 tccgcgcgag atatggcgct gatcggccag gcattgatcc gtgacgtacc gaatgaatac
    15961 tcgatctata aagaaaaaga atttacgttt aacggtattc gccagctgaa ccgtaacggc
    16021 ctgttatggg ataacagcct gaatgtcgac ggcatcaaaa ccggacacac tgacaaagca
    16081 ggttacaacc ttgttgcttc tgcgactgaa ggccagatgc gcttgatttc tgcggtgatg
    16141 ggtggacgta cttttaaagg ccgtgaagcc gaaagtaaaa aactgctaac ctggggcttc
    16201 cgtttcttcg aaaccgttaa cccactgaaa gtaggtaaag agttcgcctc agaaccggtt
    16261 tggtttagtg attctgatcg cgcttcgtta ggggttgata aagacgtgta cctgaccatt
    16321 ccgcgtggtc gcatgaaaga tctgaaagcc agctatgtgc tgaacagcag tgaattgcat
    16381 gcgccgctgc aaaagaatca ggtcgtcgga actatcaact tccagcttga tggcaaaacg
    16441 atcgagcaac gcccgctggt tgtgttgcaa gaaatcccgg aaggtaactt cttcggcaaa
    16501 atcattgatt acattaaatt aatgttccat cactggtttg gttaaaaatt gaacacttga
    16561 aagtgtaatt tccgtcccca tatactaagc atcagtaaaa aaactcccac cttctggcgg
    16621 gagttgctat ttaattacgt tacgccggag ctgacatgaa aaccaaactt aacgaactgc
    16681 ttgaattccc tactcctttt acttacaaag ttatggggca ggcgttacct gagctggttg
    16741 atcaggtggt tgaagtggta cagcgccatg cgccaggtga ctacacccca acggtaaaac
    16801 caagcagcaa aggcaactac cactcggtat ctatcactat caacgccact catatcgagc
    16861 aggttgaaac actgtatgaa gaactgggca aaatcgatat tgtccgcatg gttctgtaac
    16921 tcgcttctcc gttacccggc tcctggtcgg gtaactctcc tctcctcgct gtgatatact
    16981 tttcccccac ttttactcat tctccacgga gatgccgttt tgtatcagga taaaattctt
    17041 gtccgccagc tcggtcttca gccttacgag ccaatctccc gggctatgca tgaattcacc
    17101 gatacccgcg ataatagtac ccttgatgaa atctggctgg tcgagcacta tccggtattc
    17161 acccaaggtc aggcaggaaa agcggagcac attttaatgc cgggtgatat tccggtgatc
    17221 cagagcgatc gcggtgggca ggtgacttat cacgggccgg gacaacaggt gatgtatgtg
    17281 ttgcttaacc tgaaacgccg taaactcggt gtgcgtgaac tggtgacctt gcttgagcaa
    17341 acagtggtga ataccctggc tgaactgggt atagaagcgc atcctcgggc tgacgcgcct
    17401 ggtgtctatg tcggggaaaa gaaaatttgc tcactgggtt tacgaattcg acgcggttgt
    17461 tcattccacg gtctggcatt aaacgtcaat atggatcttt caccattttt acgtattaat
    17521 ccttgtgggt atgccggaat ggaaatggct aaaatatcac aatggaaacc cgaagcgacg
    17581 actaataata ttgctccacg tttactggaa aatattttag cgctactaaa caatccggac
    17641 ttcgaatata ttaccgctta attccataca tcaatggccc aatttacact gggtcattac
    17701 tcataatacc tgctaatgct ttacatctcc ttcgcgtttg tatattttct ttatcacttg
    17761 cagaaatgcg gtgccacgcc ttgctccgta actcacccca ttgattaacc tattgaaata
    17821 gttgattatg gtgagcaaaa gcgttccgtt ccaattagac ataagattat tgtaattttt
    17881 taaatattat ttttccatga ataaaattgg agtaagtgcg tggatagtaa taatcaaatt
    17941 gaaccctgtt taagcagaaa gtcgtcagaa ggtaaaccac aaatatttac aaccttacga
    18001 aatattgatc ttaaccttct gactattttt gaagctgtat atgtacataa agggatcgtt
    18061 aatgcagcga aagtgcttaa tctgaccccc tcggcaatca gtcagtctat tcagaaactg
    18121 cgcgttatat tccctgaccc attgtttatt cgcaaaggcc agggtgtcac tcctaccgca
    18181 tttgcgatgc atctacatga gtatatcagt cagggccttg agtccattct tggcgcgctg
    18241 gatatcgaag gaagctatga taagcaacga acgataacta ttgcgaccac tccctcggtc
    18301 ggagccctgg tccttcctgt catctatcgg gcgattaaaa ctcactatcc gcagctttta
    18361 ctgcgcaacc cgcccatcag cgacgcggaa aaccaactca gtcagtttca aaccgatctc
    18421 atcatcgata acatgttttg caccaatcgt acggtgcaac atcatgttct gttcaccgac
    18481 aatatggtgt taatttgccg caagggaaat ccactactct ctttagaaga tgacagagag
    18541 actatcgaca acgctgcgca tgtacttctg ttaccggaag ggcaaaattt cagcggtctg
    18601 cggcagagag ttcaagagat gtttccggac cggcaaatca atttcaccag ctacaacatt
    18661 ttgacaatcg ctgcactggt tgccaacagt gacatgttag cgattattcc cagccgtttt
    18721 tataacctgt ttagtcgttg ctggccgctg gaaaaactgc cttttccgtc cttaaatgag
    18781 gagcaaatag atttctctat ccactacaac aaattcagcc tgcgtgatcc catcctgcac
    18841 ggagtaatag atgttatccg taatgcattt tgaaaaagta ttgttacagc cagatcacag
    18901 tgcagaacga taaaatggca caatccaaca acaattttac attttcgcga ccgctttggc
    18961 tgctttcagg tccgtttcaa tgatatactg ccagtcgtta attcaaaaat agttgataat
    19021 tacaacaatc tattgaattg aaacgctttc cttcgtaatt cgcaactgga acacgcacgc
    19081 tatgagtaaa cccattgtga tggaacgcgg tgttaaatac cgcgatgccg ataagatggc
    19141 ccttatcccg gttaaaaacg tggcaacaga gcgcgaagcc ctgctgcgca agccggaatg
    19201 gatgaaaatc aagcttccag cggactctac acgtatccag ggcatcaaag ccgcaatgcg
    19261 caaaaatggc ctgcattctg tctgcgagga agcctcctgc cctaacctgg cggaatgctt
    19321 caaccacggc acagcaacgt ttatgatcct cggcgctatt tgtacccgcc gttgtccgtt
    19381 ctgtgatgtt gcccacggtc gcccggtagc tcctgatgcc aatgaaccag tgaaactggc
    19441 gcagaccatt gccgatatgg cgctgcgtta tgtggttatc acctccgttg accgtgatga
    19501 cctgcgcgat ggcggtgccc aacactttgc ggattgtatt actgccattc gggaaaaaag
    19561 cccgcaaatc aaaattgaaa ctctggtgcc ggatttccgc ggtcgaatgg atcgtgctct
    19621 ggatattctg actgcaacgc ccccagatgt gttcaaccat aacctggaaa acgtaccgcg
    19681 tatttaccgg caggtacggc ctggcgcaga ttacaactgg tcgctgaagc tgctggaacg
    19741 ctttaaagaa gcgcatccgg aaatcccgac caagtctggt ctgatggtgg gtctgggtga
    19801 aaccaatgaa gaaattattg aggtaatgcg cgacctgcgc cgtcatggtg tgacgatgtt
    19861 aacgctgggg caatatttgc agccaagccg ccatcacctg ccggttcaac gttacgttag
    19921 cccggatgag ttcgacgaaa tgaaagccga agcgctggcg atgggcttta cccatgctgc
    19981 atgcggtccg tttgtccgct cttcttacca cgccgatttg caggcgaaag ggatggaagt
    20041 taagtaatcc tgtaataatt acttacatct gtctgataaa aaacccgctt tttgaagcgg
    20101 gttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca ctctttatga
    20161 gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc atcgtcatca
    20221 ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag cgtacgtaac
    20281 ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt ggtaatacta
    20341 atctcaccca tagatacctt cttgacatat aaccggcaac agaacgcgcc ttaaaaacca
    20401 gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca aggtgaatca
    20461 agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg ttgtttaaga
    20521 tgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc ataatgagtg
    20581 caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc cggctttgcc
    20641 cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta tccagcgcac
    20701 gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccacgaacc caggcggcag
    20761 gaagtaccag gatttcagct ccctgtaaag cctgtgccag cgccagctct ggaaagcgta
    20821 agtcataaca ggtcatcaga ccgaccttca tcccttccac ctccagtaac ggagctattt
    20881 cattaccagc atcaacatgg cgtgattcct gaatggcaaa tgcatcatag agatgcagtt
    20941 tggcataacg ggcgacgatg tttcctgcct gaagtgccac cagcatattc catgcgcgcc
    21001 ccggcgttga aggaagatga atcgtcagaa ttgtcgtcat catgttacgt ttactttctc
    21061 gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc gatagatctg
    21121 catcatgatc atcgcgcgcc agcaatgctt ccggcaggac aaacagcgat acgtcgtttt
    21181 ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac acagatgtaa
    21241 cagcaaactg tcctgcagca accagcattg ccacctcctg tcagagtcgt aaaattcgcc
    21301 aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca ggattttgca
    21361 gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt agcgagatgg
    21421 ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac gctggcagca
    21481 aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag gatgacgaac
    21541 attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct aacaaccttc
    21601 tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg ctgggcatta
    21661 ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc attctggctg
    21721 ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag cgggttttga
    21781 attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc tttggcaccg
    21841 ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc attagtctgg
    21901 attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat gaaaccgaat
    21961 cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa aattaccttt
    22021 acatgaaaaa tagacacaaa tgctgtgtcg gttatcagta caccaattgt ggtacgcttt
    22081 gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg ttgcttattt
    22141 tagttattga ttttaaagaa gttactaaaa cttcatttgc ctcaaaactg aaagcgcatc
    22201 caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat ctgaaaagtc
    22261 actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat gtaattccag
    22321 ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt ttcatccgcc
    22381 agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt gttccacgag
    22441 tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca acgcgacagg
    22501 ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt gtcgtaagcg
    22561 aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg ctgttgccag
    22621 gtttgtgcaa tattttctct aaacgttgtc atcctctcat ctgcgttagc aaaaacttta
    22681 ccaacgctga ttaactgaat aaaaacaaag gaaaagatag cgacatattt actcacgttc
    22741 atttgtgacc ataaaacatt tatcaaaaat ctactactag catagcaaag ctacaattaa
    22801 cataacctta atagacccaa cataaagaat aatctgaata gctgctctat ttatttccgc
    22861 ttgttcaagt taaatatttc cgcgcatcga ttaaagatga tgcgtcgttt tctgcgatgg
    22921 gaatagtcaa aaaagaaaaa ccaagtcttt tttgatgaca aaatgcaatc aaggaaaaat
    22981 tattttattt ttttaacgag atacaacaat catcttaacg aagtatataa tatatatcgt
    23041 caacccatgc tcatattaga ttattttttg agcgcacagc cgcagctaac accattgcaa
    23101 ttaacaaatt tgcatcaatc caccatcaat ttacacacat tattatgtga taattgccaa
    23161 ccgctaaata tgcgttttgt tatctatgta taaaaacagc aacttcaatg tcttaatggc
    23221 agtttttctt gattttaatc agcattcatc gccaatttat tgggcatatt ttttccttaa
    23281 gctttaggaa gtttttatta tttactttgg gcctggagac aggaaaaatt atgctgacat
    23341 tcattgaact ccttattggg gttgtggtta ttgtgggtgt agctcgctac atcattaaag
    23401 ggtattccgc cactggtgtg ttatttgtcg gtggcctgtt attgctgatt atcagtgcca
    23461 ttatggggca caaagtgtta ccgtccagcc aggcttcaac aggctacagc gccacggata
    23521 tcgttgaata cgttaaaata ttactaatga gccgcggcgg cgacctcggc atgatgatta
    23581 tgatgctgtg tggctttgcc gcttacatga cccatatcgg cgcgaatgat atggtggtca
    23641 agctggcgtc aaaaccattg cagtatatta actcccctta cctgctgatg attgccgcct
    23701 attttgttgc ttgtctgatg tcactggccg tctcttccgc aaccggtctg ggtgttttgc
    23761 tgatggcaac cctgtttccg gtgatggtaa acgttggtat cagtcgtggc gcagcagctg
    23821 ccatttgtgc ctccccggcg gcgattattc tcgcaccgac ttcaggggat gtggtgctgg
    23881 cggcgcaggc ttccgaaatg tcgctgattg acttcgcctt caaaacgacg ctgcctatct
    23941 caattgctgc aattatcggc atggcgatcg cccacttctt ctggcaacgt tatctggata
    24001 aaaaagagca catctctcat gaaatgttag atgtcagtga aatcaccacc actgcccccg
    24061 cgttttatgc cattttgccg ttcacgccga tcatcggtgt actgattttt gacggtaaat
    24121 ggggtccgca attacacatc atcactattc tggtgatttg tatgctgatt gcctccattc
    24181 tggagttcct ccgcagcttt aatacccaga aagttttctc tggtctggaa gtggcttatc
    24241 gcgggatggc agatgcgttt gctaacgtgg tgatgctgct ggttgccgct ggggtattcg
    24301 ctcaggggct tagcaccatc ggctttattc aaagtctgat ttctatcgct acctcgtttg
    24361 gttcggcgag tatcatcctg atgctggtat tggtgattct gaccatgctg gcggcagtca
    24421 cgaccggttc aggcaatgcg ccgttttatg cgtttgttga gatgatcccg aaactggcgc
    24481 actcttccgg cattaacccg gcgtatttga ctatcccgat gctgcaggcg tcaaacctgg
    24541 gtcgtaccct atcacccgtt tctggcgtag tcgttgcggt tgccgggatg gcgaaaatct
    24601 caccatttga agtcgtaaaa cgcacctcgg tgccggtgct tgttgggcta gtgattgtta
    24661 tcgttgctac agagctgatg gtgccaggaa cggcagcagc ggtcacaggc aagtaaatag
    24721 taatgccgga ggcaagtttc ctccggcttt atcatgtcac ccactgtggt atatgcgttg
    24781 tggtctgcca actttgccgt gaacaatttc agcaataatc agatggcggc tggcgcaata
    24841 ttcaagataa cgcctggcag tggtgcggct gatggtcagt gcctgcgcca ccgtttctgc
    24901 cgtatgttgc acaccaggct ctttaaacag ttttcgcacc gcgtttagcg tcaaggggtc
    24961 aatgccggtc ggcagttcgt ctttgggttc accgcgagca taggcattaa acatctcatc
    25021 aatttgcttc tggctggcgc tatcaatact ttccagcata tgtttacact ggcggaaacg
    25081 ggttagcgtt tgccccagtg tattgattca cttgaagtac gaaaaaaccg ggagaacatt
    25141 ggattattcg ggatctgatg ggattagatt tggtggggct tgcaagcctg tagtacaaat
    25201 tttaggc
//
LOCUS       DAJVQH010000049        24851 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJVQH010000049 DAJVQH010000000
VERSION     DAJVQH010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.049"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            72..353
                     /locus_tag="OQM87_002072"
     CDS             72..353
                     /locus_tag="OQM87_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCS1508213.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            319..1396
                     /locus_tag="OQM87_002073"
                     /pseudo
     CDS             319..1396
                     /locus_tag="OQM87_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1789..2130)
                     /locus_tag="OQM87_002074"
     CDS             complement(1789..2130)
                     /locus_tag="OQM87_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCS1508214.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2143..3015)
                     /gene="copD"
                     /locus_tag="OQM87_002075"
                     /pseudo
     CDS             complement(2143..3015)
                     /gene="copD"
                     /locus_tag="OQM87_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3019..3393)
                     /gene="yobA"
                     /locus_tag="OQM87_002076"
     CDS             complement(3019..3393)
                     /gene="yobA"
                     /locus_tag="OQM87_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCS1508215.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3532..3762
                     /gene="holE"
                     /locus_tag="OQM87_002077"
     CDS             3532..3762
                     /gene="holE"
                     /locus_tag="OQM87_002077"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCS1508216.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3864..4520
                     /locus_tag="OQM87_002078"
     CDS             3864..4520
                     /locus_tag="OQM87_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCS1508217.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4544..5206
                     /gene="exoX"
                     /locus_tag="OQM87_002079"
     CDS             4544..5206
                     /gene="exoX"
                     /locus_tag="OQM87_002079"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCS1508218.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5203..7263)
                     /gene="ptrB"
                     /locus_tag="OQM87_002080"
     CDS             complement(5203..7263)
                     /gene="ptrB"
                     /locus_tag="OQM87_002080"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCS1508219.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(7472..8131)
                     /locus_tag="OQM87_002081"
     CDS             complement(7472..8131)
                     /locus_tag="OQM87_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCS1508220.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8458..8814)
                     /locus_tag="OQM87_002082"
     CDS             complement(8458..8814)
                     /locus_tag="OQM87_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCS1508221.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="OQM87_002083"
                     /pseudo
     CDS             complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="OQM87_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9305..10483
                     /gene="purT"
                     /locus_tag="OQM87_002084"
     CDS             9305..10483
                     /gene="purT"
                     /locus_tag="OQM87_002084"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCS1508222.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10538..11179)
                     /gene="kdgA"
                     /locus_tag="OQM87_002085"
                     /gene_synonym="eda"
     CDS             complement(10538..11179)
                     /gene="kdgA"
                     /locus_tag="OQM87_002085"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCS1508223.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11216..13027)
                     /gene="edd"
                     /locus_tag="OQM87_002086"
     CDS             complement(11216..13027)
                     /gene="edd"
                     /locus_tag="OQM87_002086"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCS1508224.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PISKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13262..14737)
                     /gene="zwf"
                     /locus_tag="OQM87_002087"
     CDS             complement(13262..14737)
                     /gene="zwf"
                     /locus_tag="OQM87_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCS1508225.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15075..15944
                     /gene="hexR"
                     /locus_tag="OQM87_002088"
                     /gene_synonym="yebK"
     CDS             15075..15944
                     /gene="hexR"
                     /locus_tag="OQM87_002088"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCS1508226.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16072..17514
                     /gene="pyk"
                     /locus_tag="OQM87_002089"
     CDS             16072..17514
                     /gene="pyk"
                     /locus_tag="OQM87_002089"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCS1508227.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="OQM87_002090"
                     /gene_synonym="msbB"
     CDS             complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="OQM87_002090"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1508228.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="OQM87_002091"
     CDS             complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="OQM87_002091"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCS1508229.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLLFD"
     gene            complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="OQM87_002092"
     CDS             complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="OQM87_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCS1508230.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21085..21840
                     /gene="znuC"
                     /locus_tag="OQM87_002093"
     CDS             21085..21840
                     /gene="znuC"
                     /locus_tag="OQM87_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCS1508231.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21837..22622
                     /gene="znuB"
                     /locus_tag="OQM87_002094"
     CDS             21837..22622
                     /gene="znuB"
                     /locus_tag="OQM87_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCS1508232.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="OQM87_002095"
     CDS             complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="OQM87_002095"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCS1508233.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="OQM87_002096"
     CDS             complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="OQM87_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCS1508234.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(24538..24603)
                     /gene="yobI"
                     /locus_tag="OQM87_002097"
     CDS             complement(24538..24603)
                     /gene="yobI"
                     /locus_tag="OQM87_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCS1508235.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24674..>24851
                     /locus_tag="OQM87_002098"
     CDS             24674..>24851
                     /locus_tag="OQM87_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508236.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLS"
BASE COUNT         6167 a         6287 c         6104 g         6293 t
ORIGIN      
        1 ttattaaaaa tcaaccacac ccgccagtat tctgtatatt tactggcggt catatcgtga
       61 gaggtatggc aatgaatctt gtgacactca aaacgtgggg aaaactcaga tatccggata
      121 acccaccatc aatatcaacg ctgagacgat gggcaaggaa tggaaacatt tatcctgcac
      181 ctgaactaca cgggaggagt tacagggtgg ttccggaggc tttctatatc aacccaaata
      241 aggttgatac cgatataaca caccatcagc ctaatgggcg acaagggaga gacagtccgt
      301 taatggagaa gttaaaacat gcagcggaaa aaatacgatc ccaatttgcc tagaaactta
      361 acatatcgaa ggagagacaa agcatattac tggcgcaacc ctctgacgaa agaagaattt
      421 acactaggta aaatttcaag aagagatgca gtagcgcagg caattgaagc aaatcattat
      481 atatacaaaa actactctcc tgctgcccta attgaaaagc ttaaaggggt cgactcattt
      541 actatggcag actggattga acgttacaaa acgattctta taaggagaaa agcgtccaga
      601 aatacttata aaactcgggt aaatcaactg gtgacaataa aagaaaaatt aggaggggtt
      661 ttactgacag aaataaccac tcgccatatc gccgagtttc ttgatttgtg gattgaagga
      721 gggaaaaaca cgatggcagg atcaatgcgt tctgtgttat ctgatatgtt ccgcgaagcc
      781 attgttgaag gacgtatatc tcaaaatcca gtaacgccaa caagagcacc gaaaatagta
      841 gttacaagag aacggctgaa actaaagaca tacaactgca tcagggaggc agcagatcaa
      901 cttccggcat ggttcccatt agctatggac ttagcccttg taacaggaca acgtcgcgaa
      961 gacataacga atatgcggtt cagtgatatt tatgatgatc gtctccacat caggcaaatt
     1021 aagacaggaa tgatgattgc tatccccctg tcactcagcc ttcctgtcgc tggtttacga
     1081 cttggtgcag tagttgaaca gtgccgcatg gtaagtaggg ggggattatc taatcagtgc
     1141 cgggattaga aaaaacagcc ctgacggcag cattcacccg gacggcctga caaagaaatt
     1201 tgtcgcagcc agaaaattaa caggtatcct gttcagtgaa aacccaccaa cttttcacga
     1261 aatcagaagc ctggctggac gattgtacaa agaaatatgt ggagaagaat ttgctcagcg
     1321 tctacttggc cacacatcgg agaagacaac aaaaatgtat cttgatgaga gagaaaaaac
     1381 gtacttactg ctctgatttt aacgtaaatg gattgttaaa tgtattttgg ttgtgatata
     1441 accaaaaaag accggaatac agaaattcga gtaaatttcg gggaatttcg gggagacgtt
     1501 tgcaactaat tgattttaaa tacaattaaa aaaagaccga atacgattcc tgtattcggt
     1561 ccagggaaat ggctcttggg agagagccgt gcgctaaaag ttggcattaa tgcaggctta
     1621 gttgccttgc cctttaagaa tagatgacga cgccaggttt tccagtttgc gtgcaaaatg
     1681 gtcaataaaa agcgcggtgg tcatcagctt aaatgttaaa aaccgcccgt tctggtgaaa
     1741 gaactgaggc ggttttttta ttggaaatca aaaggctatt ttaggtaatt aacagagttt
     1801 ttcagctcgt tctataaacg gtgccagact cattttttcg ccgggattgt taggatcatc
     1861 aatctgaatc accgaaatgg gttgggcatt ggtcttccca ctggcaactt ccttttgtgc
     1921 gatatcgttt aaaggatact gcacgagggt acttgggttg atgacataca aagcattacc
     1981 cggtcggcaa gtcagcatta cctcttcgcg attaaacgcc catttgtctt tacccacttc
     2041 aaaacgactc acggttatca cctgtggagc cgccagcgcc gctgcagaac tggtgagtaa
     2101 cagaaacgcc agaatacttt ttttcatcat tttcgtaatc catcaaaaag gttcgagagt
     2161 cgcaagcagg ctgatgatca ccagcactac cgcacctatc gcccattcga gtttagtcat
     2221 ccacacaaac cagggtgccg cacgatcttc atcctgcctc atgcgcggta caagaacata
     2281 acgattagcc agcgcaatga ccaccataat catcaccagg atggctttga gcaataagag
     2341 ttggccctag taagtcgtaa gcgtgggggg aaatccggta atcaacaatg cattaagcac
     2401 gccgcttgcc agtacgccga tcaccgcaaa atgcccgcac caggaaaaac gcatcagcgc
     2461 ctgaatagcc tggtgtcgcc aacggccttt gatgagttgc atacaccaga gcaccggcag
     2521 taatccccca aaccacgccg ccgcacaaat caggtgaatc gcgtgattag tctgatggat
     2581 tttcgctgtt accccttcat tcagcgtcgc atgccccact cccgccagca gaataaattg
     2641 cgcggtagtg agcataaaca acaaacgtgg catattccgc ggttgcataa gggcgacgat
     2701 caacgttacg agggcgagaa caatttgcca taaccagacg ccaccaaact gcgtttgcaa
     2761 aacagcctgc caaatgtttg gcgaaaatac atctgtccat cccgttccca tcaacccgcc
     2821 ctgaacggcg agcattgcag tggcactgat caaactccag acggcagcgt gctgttgtaa
     2881 acgcagagaa cgcttcgtca gcaagcgacg aatcattaag ggggccagcc aggcaccata
     2941 cattgcaaag ccaaagacca gcatcaacga ggtgaaatgg ataaatcgca gcgcgatcca
     3001 ggtaaacgcc agcatgattt atttcacgct aaaggtgtag tgtcctttcg ttttatgccc
     3061 gtccaccgaa acaacatgcc agtcaacggt gtaggttccg ggtttcagcg aatcggccag
     3121 tggaacaatc agttgctttt ggtcctgctc attccgcttc gctggtaatg ttttaatatt
     3181 ttcgtttttt ggccccgtga tttttgcacc actgaatcct gtttcaacgc cttccgagaa
     3241 gtttaaagtg attgcctgcg gggcagctgt cacttgcgcg tttgccgcag gatactgatg
     3301 cgttaaatgc gcatgtgccc agacggaagg tgtcaccaac gaagtggtca ggattgccag
     3361 cgcgtagcga agggagcgtg cagttgaagc catattatct attccttttt gtaataactt
     3421 ttttacagag cataaccttg tctaatgtct gagtcgagga tcatcaattc cggcttgcca
     3481 tccaggctca ctcttagtaa cttttgcccg cgaatgatga ggagattaag aatgctgaag
     3541 aatctggcta aactggatca aacagaaatg gataaagtga atgtcgattt ggcggcggcc
     3601 ggggtggctt ttaaagaacg ctacaatatg ccggtgatcg ctgaagcggt tgaacgtgaa
     3661 cagccagaac atttgcgcag ctggtttcgc gagcggctta ttgcccaccg tttggcttcg
     3721 gtcaatctgt cacgtttacc ttacgagccc aaacttaaat aaaacttata cagagttaca
     3781 ctttcttaca taacgcctgc taaattatga gtattttcta aaccgccccc ataatttgca
     3841 gtcattttga aaaggaagtc attatgtcgt tctggaaagt tgcagcagcg caatatgagc
     3901 ctcgtaaaac ctcactcacc gaacaggtag ctcatcatct ggagtttgtg agggccgccg
     3961 ccaggcaaca atgtcagctg ttagtttttc catcgctttc tttactgggg tgtgattatt
     4021 cgcgacgtgc ccttcctgcc ccacccgatc tttcactgtt agacccgctt tgttatgccg
     4081 caacgacctg gcgaatgacc atcattgccg gccttcccgt tgaatataac gatcgcttta
     4141 ttcgtggaat tgcggtgttc gcaccgtggc gaaaaacgcc tgggaactac catcagagtc
     4201 atggtgcatg tctgggccgt cgttccagaa ccatcacggt ggtcgatgaa caaccgcaag
     4261 gtatggatat ggacccgacc tgttcactgt ttacgacagg gcaatgtctg ggagaacccg
     4321 acctgctggc gtcggcccgt cgcttacaat ttttttcaca tcagtactcg attgccgtgc
     4381 tgatggcaaa tgcccgtggt aacagtgcac tatgggatga atatggtcgt ctcatcgttc
     4441 gcgccgatcg tggttcatta ttgttagtcg gtcagcgttc atcacagggt tggcaaggcg
     4501 atatcattcc attacgctag gctttttcgg cctggagcat gccatgttgc gcattatcga
     4561 tacagaaacc tgcggtttgc agggagggat cgttgagatt gcctctgttg atgtcattga
     4621 cggaaaaatc gtcaacccca tgagcctcct ggtgcgcccc gatcgtccta ttagtccaca
     4681 agcgatggcg attcatcgca tcaccgaagc catggtcgcc gataaaccgt ggattgaaga
     4741 tgtgatccca cactattacg gtagtgaatg gtatgtcgcg cacaacgcca gctttgaccg
     4801 ccgcgtactg cctgagatgc ccggtgagtg gatttgcact atgaaactgg cccgtcgttt
     4861 gtggccaggg ctcaagtaca gcaatatggc gttatataaa acacgcaagc tcaatgtaca
     4921 gacaccgccg ggcctgcatc atcaccgcgc gttgtatgac tgttatatca ccgccgcgtt
     4981 gcttatcgat attatgaaca cctccggctg gacggcagaa cagatggccg atatcaccgg
     5041 acgtccgtcg ttgatgacga ccttcacctt tggcaaatac cgtggcaaag cggtttccga
     5101 cgttgccgaa cgcgatccgg gctatctgcg ctggttattt aataacctgg acagcatgag
     5161 cccggagctg cgtttaacac tgaaacatta tctggaaaat acttaatccg caggcgtagt
     5221 gggtaatgtt ccctgcgcca gcgcgaccag aaaagcatat tccatcgcta cgccttcgta
     5281 cgatttaaag cggccagatt taccgccatg gcctgagtcc atgtcggtac agagcaataa
     5341 aagatggtca tcggttttca gctcgcgcaa tttagcgacc cattttgccg gttcccaata
     5401 ttgcacctga gaatcgtgta aaccggtcgt taccagtaaa tgcggataag cctgtgcggt
     5461 gacgttgtca tacgggctgt agcttttcat gtactcgtaa tattgcggat cctgcggatt
     5521 tccccactct tcaaactcac cagtggtaag aggaattgat tcatcaagca tcgttgttac
     5581 aacatcaaca aacggtacct gggcgataac gccgtggaat aattccgggc gttgattaat
     5641 tgcaacgccc atcaacatgc cccccgcact tccgcccatc gcataacaga gcgaaggaga
     5701 gccatagccc agttttaaca atgcatcgca ggcatcaaga taatcattaa acgtattttt
     5761 cttcttcaga aatttaccgt cttcgtacca ttgttgcccc agctcaccac cgccgcgaac
     5821 atggacaatg gcgtagacaa agccacgatc taacaaactc aagcggctaa aactgaaatc
     5881 ggcatcaata cttgcgccgt aagaaccata gccatacacc agcaacgggt tgtgtccttt
     5941 gcgaaaatgt ttgcgatgat agaccagcga aaccggaact tcgacgccat cacgggcgac
     6001 tatccacagg tgttcactgc ggtaattcgc cgcatcaaaa ccgggcactt ctgtttgttt
     6061 taatacacga cgctcaccgg tatccatatc cagttcaaac aaagtgtctg gtgtggtcat
     6121 ggaagaataa ccataacgca atcgcgcggt ttcaggttct ggattgtagg caatccaggt
     6181 cacataggcc ggatcatcaa aggcaatgcc aatgacttcc cgggtcttgc ggttaatttg
     6241 gcgcaaactg gttaacccgc gctgacgctc ttcaaccacc agccagtcgg taaacagcgt
     6301 aaacccttcc agcatgatgt tttcacgtgg cggaattaac tcttcccact gttgctcatc
     6361 acgcatacgg gtacggtata agccaaagtt tttgccgtgg cgattggaac gcagataaaa
     6421 acgatgctgg tagtgatcaa ggctgtattc gtgatctttg cggcgcggca gaaaaacaaa
     6481 cggctcggca tcggccattt ccgcgtccag caggcgaact tcactggtgg tggcgctggc
     6541 cagatgaatg acgacatagt gcttcgacgt cgttttatgc aggctgacat aataggtatc
     6601 gtctttttct tcgtagatca gtgtatcttg cgatgctggc gtaccgatgg cgtgacgcca
     6661 gacctgataa ggcagcagcg tcaccggatg cttgcgaaca tagtagaaag tccaggagtc
     6721 atttgcccag acaaagctgg gttcaacgtt atccagcagt tccgggtacc agttacctgt
     6781 ttccagatta cgaaaacgaa tgccgtactg gcgtcgggaa agaaaatctt ctgccagcgc
     6841 cataatggtg ttatcgggcg taatcgccat tccgcccatc gaataaaact cactatgagc
     6901 tgcgcgcttg ttggcatcga gcaatgtttc ccactcatcc cactcttcgc tgaatgccga
     6961 ttgacgctgg tagatagcat attcacagcc tggttcataa atatgccgat agcggtagcc
     7021 attttttatg tagggcgcag aaacttctct ttgcggaatg cggtcgatga tttcctttaa
     7081 gatgcgatcc tgcaaggctt gttgtgaggc catcacccga tgaccgtaac tattttcttg
     7141 ttgcaggtag tccaggactt ctggctgaga acgcgtatcg tcccgcagcc agtagtaatt
     7201 atcgatgcgc gtatcgccat gaagcgtcat ggcgtgggga atgcgggcgg cttttggtag
     7261 catgttattg ttctttctgg ttgaaacatc ttataagggt ggcaaaactc accggggatg
     7321 caagcgaaac agggtcgtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg
     7381 ttatgcagac agagaaaatc agcctgttca aatggctgtg cggttctgga tagcccgaaa
     7441 tagtcaactt caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt
     7501 cgagatcgcg ttcaatgcct tcacgaacat cctgggggat tttcagcgcg tcacccagtg
     7561 cattcaggta actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat
     7621 agatttccag cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg
     7681 gttgttcgat tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca
     7741 attgctggtc gattgccgca cgttcattgg catcaatatg accatcactt ttagcggcaa
     7801 agaccagcgc aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa
     7861 actgcggttc gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg
     7921 tacccgctac cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt
     7981 tacgtgctga tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctggcacta
     8041 acagtttgcc caatccctga tccgcagacg aggaggtaga agaactgctt tgcccaagca
     8101 gggattgcag ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc
     8161 ctgtagcata gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata
     8221 agcggggcgc taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat
     8281 caggtgataa gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg
     8341 ctgattgccc gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg
     8401 gacggacaag gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta
     8461 acgccgctga tattccgcca ttcccgcttt gcagtccacg ctgacctggt aatgaatatc
     8521 ggcactttta ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc
     8581 ctgcaaactg acccaggcca cgggatcggc ctgaccgaca attttttgat catctgccca
     8641 gcgcgccacg cgattttgtt gataatcacg ttttacgctc gcggcaattc cggtagcatc
     8701 cagaccttca cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac
     8761 tgatgcgcag gcagaaacca acaaccgccc taaaaacgcc cctctttttt tcatgttttt
     8821 ctccatagca caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct
     8881 tcaggccgcg tgtgaggcat cttcctcttc ggattcagca ccgacgacca gtggggacgg
     8941 taatttgccg gttttcagaa tggtgctcag catatctttt tgttctgcca gccatagcga
     9001 aagggcttca cgttgctcgt cttccatttg cactggagaa tttgtcagct aggtgtcaag
     9061 cagatccgcc gtatcaagca ttttgtcata agcatcggct tcctttttgc tggtaaacga
     9121 cattttctct tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct
     9181 ctcataataa ctgtgatttt atacagtata tttcttttcg gttgagaaat caacatcagc
     9241 aataaagaca cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt
     9301 cgttatgacg ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg
     9361 ctccggtgaa ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat
     9421 tgccgtcgat cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat
     9481 taatatgctt gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat
     9541 cgtgccggag atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact
     9601 gaatgttgtc ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg
     9661 cctggcggca gaagagctgc agctgcccac ttccacttat cgttttgccg atagcaaaag
     9721 ccttttccgc gaggcggttg ccgccattgg ctatccctgc attgtaaaac cggtgatgag
     9781 ctcttccggc aaggggcaga cgtttattcg ctctgcagag caacttgctc aggcatggga
     9841 atacgctcag caaggcggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa
     9901 gtttgacttc gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc
     9961 accagtaggt catcgccagg aagatggcga ctaccgtgaa tcctggcaac cacagcaaat
    10021 gagcccgctt gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg
    10081 cggttatggt ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga
    10141 ggtctcccct cgtccacatg ataccgggat ggtgacgtta atttctcaag atctctcaga
    10201 gtttgccctg catgtacgtg ccttcctcgg acttcctgtt ggcgggatcc gtcagtatgg
    10261 tcctgcagct tctgccgtta ttctgccaca actgaccagt cagaatgtca cgtttgataa
    10321 tgtgcagaat gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga
    10381 tggcagccgt cgtctggggg tggtactggc tacttcagag agtgttgttg acgccattga
    10441 acgcgcgaag cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaacgct
    10501 acaaaaatgc ccgatcctag atcgggcatt ttgactttta cagcttagcg ccttctacag
    10561 cttcacgcgc cagcttagta atgcggtcgt aatcgcccgc ttccagcgca tctgccggaa
    10621 ccagccagga accaccgatg cacagcacgc ttttcagtgc caggtagtca cggtagttag
    10681 ccggagaaat accacccgtc gggcagaaac ggacctggga gaacggaccc gcgatcgcct
    10741 gcagggcttt cacgccgccg ttagcttcag ccgggaagaa tttgaactct ttcaaaccgt
    10801 agtccatacc cagcatcagt tcggaaacag tgctgatccc cggaatcaga ggaatagtcc
    10861 cttcggtagc agctttcagc agcggctcgg tcagacccgg gctaattgcg aactgtgcac
    10921 ccgcttcagt gacttctgcc agctgctgtg gattcagcac cgtaccggca cccacaatcg
    10981 cttcaggcac ttctttggcg atagcacgga tagcgtcaac tgcacactcg gtacgcagag
    11041 tcacttccag aacgcgcacc ccaccagcaa ccaacgcttt tgccatcggc accgcgtgtt
    11101 ccagtttttt taccacgata accggtacaa ccgggccggt ggtcaggatt gattctgcac
    11161 ttgttttcca gtttttcatc agagttttct ctcgcctgat tacaaatttg tcgtcttaaa
    11221 aagtgataca ggttgcgccc tgttcggcac cggacagttt ttcacgcaag gcgctgaata
    11281 attcacgtcc tgttctcacg cgtgacgcgc tcaggtcagg aatgtgcggt tcgcgagcag
    11341 ccagttccgc ttcgtctacc agcagtgtca gttcgcctgt ctgtccattc acacgaatga
    11401 tgtccccgtc gcgcactttt gccagcagcc cgccatcgta ggcttctggt gttacgtgga
    11461 tagctgacgg cactttacct gaagcgccgg agagtcgtcc atcggtaact aacgcaattt
    11521 tgaaacaccg gtccaataat acaccaagtg gcggcatgag tttatgtaat tctggcattc
    11581 cgttcgcttt tggcccctga tgacggacaa caacgacaca atcgcggtcc agcaaacccg
    11641 cttcaaaggc cggcataacg tcatgctggc tttcaaaaac aaccgctggc gcttcaatca
    11701 cctggttctc aaccggcacg gcagaggttt tcataaccgc acggcccagg ttaccgctta
    11761 acacttttgt cccaccatga tgagagaaag gttgttcgaa ggaagcgatc acattgctgt
    11821 cgagtgattt ttccgcccct tcccgccagt ccagttcacc attattcagc catggttcaa
    11881 gggtataacg aaacagacca aagcctgcca ccgtattgac atcttcatgc agcaggcctg
    11941 ctttgagcag ttcacgcacc agaaccggta cgccacctgc cgcctggaag tggttaatat
    12001 cggccggacc gttcgggtag agacgtgcca tcagcggtac aacatcagaa aggtcagaga
    12061 agtcatccca gttaatctga ataccggccg cgcgcgccat cgccaccagg tgcatggtgt
    12121 ggttagtgga accaccggtc gccaacagtg caacgatacc gttcaccacc actttctcat
    12181 cgatcatctt actgatcggc atccattcat taccattacc ggtcatgcgt gtaacctgac
    12241 gcgcagcggc agcggtcaaa gcatcgcgca gcggagaatc cggatgaaca aaagaagagc
    12301 ctggcaactg catccccata aattccacca ccatctggtt ggtgttggca gtaccgtaga
    12361 aagtacatgt tcccggcgca tggtaagacg cggcttctga ctccagtaag gccatgcggt
    12421 ccactttacc ttcggcataa agctggcgaa tacgcacttt ttctttattt ggcaaaccgc
    12481 ttgccatcgg tccagacggc acaaacaccg caggcaaatg accaaacgac agggctgcca
    12541 tcgtcagacc cgggacaatc ttgtcgcaca caccgaggaa cagagcacca tcaaacatgt
    12601 tatgggacag ccccaccgcc gcagacatcg ctatcacttc gcggcttagc agcgacaatt
    12661 ccattccatc ctgcccctgg gtgacaccat cacacatcgc cggaacaccg cccgcaacct
    12721 gaccaaccgc attcgcttca tgcagggctt tacgaatgat ttctggatag tgttcataag
    12781 gctggtgcgc ggagagcatg tcgttatagg aggtgatgat ggcgatattg ttacgcaaca
    12841 tgcttttcaa agaggctttg tcttctggct ggcaggcagc gaaaccgtgt gccaggttac
    12901 cgcatgccaa ctgcgaacga tgaacggtcg acgttttcgc ttgttctatc cgggcgagat
    12961 aagcagagcg agtctcgcgc gaacgttcaa tgattcgatt tgttacgcgt aacaattgtg
    13021 gattcataaa ggctcctgaa attgagttgt cagagcagga tgattcacaa cgcgtttcat
    13081 tcagaggatt tatgagtgaa acgcctgtaa ccggagctca tagggcaaaa acgtttcagt
    13141 cagtgtaata aaaaaagcct cgtgggtgaa tccgcacgag gcctgaaagt gtaaaaattg
    13201 ttctacaatc tgtgcaagat tatgttaccg gtaaaataac cataaaggat aagcgctgat
    13261 attactcaaa ctcattccag gaacgaccat cacgggtaat catcgccacc gaggcaacgg
    13321 gtccccaggt tccggcctga tacggtttcg gcgcatcatt gtccatcgcc cacgcctcag
    13381 taatggagtc tacccatttc caggcttctt ccacttcgtc gcgacgtaca aacagtgcct
    13441 gaataccacg catggtttcc agcagcaaac gttcataggc atccgccaga tgcgtctgat
    13501 taaaggtttc tgaatagctc agatccagct tggtgatttg caggttatgt ttgtggtcaa
    13561 ggccaggaac tttattcagt acctggatat ccacgccttc atcaggttgc agacggatag
    13621 tcagtttatt ctgcggcaga tcctgccacg actctttaaa cagattcagt tcaggtgttt
    13681 tgaaatagac cacgacttca gaacatttgg tcggcagacg tttaccagta cgcaggtaga
    13741 atggcacacc ggcccagcgc cagttatcaa tgtcgacgcg gatcgccacg aaggtttctg
    13801 tattgctact tttgttcgcg ccctcttctt ccagatatcc cggcactttt ttgccctggg
    13861 cgaagcccgc agtatattgc ccgcgtacgg ttttttcgcg tacgttggag cggtcgatgc
    13921 ggcgcaggga cttcagtact ttcacttttt catcgcggat gctgtctgcg ctcaggtcag
    13981 atggcggaga catcgcaatc atgcagagaa tttgtagcag gtggttctga atcatgtcac
    14041 gcatctgacc ggctttatca aaatagcccc agcgcccttc gatccccact tcttctgcca
    14101 cggtaatctc aacatgatca atggtgcgat tgtcccagtt attcacaaac agggagttag
    14161 caaaacgcag cgccaacagg ttcagcaccg tttctttacc aagatagtgg tcgatacggt
    14221 aaacctggca ctcctcgaag tattcgccaa cctgatcatt gatttcctgc gaggtcgcca
    14281 gcgacgtccc cagcggtttc tccatgacta cgcgtgccgg tttagcattc agttttgcct
    14341 cgccaagccc tttgcaaatt gcgccaaaag tgctgggcgg catggcaaag tagttaatag
    14401 tgatacgatt tttttgatcc agcatcgcgc cgagacggtt gaatgcagca gtgtcattga
    14461 catcgagatt acaaaaatcc agacgtgcac tcagggtgtc ccataaacct tcatcaatgg
    14521 tttctttcat gaaagtttcg agcgcctcgc ggacaacttt ggtatatgcc gctttatccc
    14581 agtcagcacg ccctacgccg ataatccggg tgtccgggtt gagctgaccg gctttttcca
    14641 gttgatacag ggaaggcagc aatttacgac gcgcaaggtc gcctttcgcg ccgaaaatga
    14701 ccaggtcaca ggcctgggct gtttgcgtta ccgccatgtc attctcctta agttaactaa
    14761 cccggtactt aagccagggt atacttgtaa ttttcttacg gtgcactgta ctgcttttcc
    14821 gagcttgcga aaactgtaaa cgcttatcca cccgtgcgat tacgggaaaa gcgcgcaaag
    14881 tgcggcaaaa ctgataaaaa aatcatcgtt ttccattcgg taaaaatctg acactgatca
    14941 tgttatgaaa aaaaataaca acttttttat ctgcttttgt cattaacacg gcacacaggc
    15001 gtaatatctg acaaaactgc atttcgattt ctttcagtgc ggaaatcgtc attacccgtg
    15061 agtctcttta catcatgaat atgctggaaa aaatccagtc tcagctggaa catttgagca
    15121 aatcagagcg caaagttgcc gaggtcattc tggcttcgcc cgataacgcg atccattcga
    15181 gtattgctgc tctggcgctg gaagccaatg ttagcgaacc gacggtgaat cgtttctgtc
    15241 gcagcatgga cacgcgcggt tttcctgatt ttaaacttca tctggcacaa agtctggcga
    15301 atggcactcc ctatgttaat cgcaacgtca atgaagatga cagcgttgaa tcatacacgg
    15361 ggaaaatatt tgagtccgca atggcaacgc tcgatcatgt ccgtcattca ctggataaat
    15421 ctgccatcaa ccgcgccgtc gacttgctca ctcaggcaaa aaaaatcgcc tttttcggat
    15481 taggctcttc tgccgccgtt gcccacgatg cgatgaataa gttctttcgt tttaatgttc
    15541 cggtggtgta ctccgatgat atcgtgctgc aacgcatgag ttgtatgaat tgtagcgacg
    15601 gagacgtggt ggtgctgatt tctcacacag gaagaacaaa aaatctggtc gagctggcgc
    15661 agctggcacg cgaaaacgac gccatggtga ttgccctcac ctctgcgggt accccgctcg
    15721 cccgggaagc aacgctggca attaccctcg acgtaccgga agatactgac atttatatgc
    15781 ccatggtttc tcgacttgca cagctgaccg tgatagatgt gctggcgaca ggatttactt
    15841 tgcgacgcgg tgcaaaattc agagataact tgaagcgggt caaggaagcg ctgaaggaat
    15901 cgcgttttga taagcagtta cttaatttaa gtgacgatcg ctaaaagcga ctgtcactgt
    15961 cctaatctta tacgacatcc gaatgagatt aatttatcgc catcgcggcg ttatttcatt
    16021 cggatttcat gttcaagcaa cacctggttg tttcagtcaa cggagtatta catgtccaaa
    16081 aggcttcgca gaacaaaaat cgttaccacg ttaggcccag caacagatcg cgataataat
    16141 cttgaaaaag ttatcgcggc gggtgccaac gttgtacgta tgaacttttc tcacggctcg
    16201 cctgaagatc acaaaatgca cgcggataaa gttcgtgaga ttgccgcaaa actggggcgt
    16261 catgtggcta ttctgggtga cctccagggg cccaaaatcc gtgtatccac ctttaaagaa
    16321 ggcaaagttt tcctcaatat tggggataaa ttcctgctcg acgccaacct aggtaaaggt
    16381 gaaggcgaca aagaaaaagt cggtatcgac tacaaaggcc tgcctgctga cgtcgtgcct
    16441 ggtgacatcc tgctgctgga cgatggtcgc gtccagttaa aagtactgga agttcagggc
    16501 atgaaagtgt tcaccgaagt caccgtcggt ggtcccctct ccaacaataa aggtatcaac
    16561 aaacttggcg gcggtttgtc ggctgaagcg ctgaccgaaa aagacaaagc agacattaag
    16621 actgcggcgt tgattggcgt agattacctg gctgtctcct tcccacgctg tggcgaagat
    16681 ctgaactatg cccgtcgcct ggcacgcgat gcaggatgtg atgcgaaaat tgttgccaag
    16741 gttgaacgtg cggaagccgt ttgcagccag gatgcaatgg atgacatcat cctcgcctct
    16801 gacgtggtaa tggttgcacg tggcgacctc ggtgtggaaa ttggcgaccc ggaactggtc
    16861 ggcattcaga aagcgttgat ccgtcgtgcg cgtcagctaa accgagcggt aatcacggcg
    16921 acccagatga tggagtcaat gattactaac ccgatgccga cgcgtgcaga agtcatggac
    16981 gtagcaaacg ccgttctgga tggtactgac gctgtgatgc tgtctgcaga aactgccgct
    17041 gggcagtatc cgtcagaaac tgttgcagcc atggcgcgcg tttgcctggg tgcggaaaaa
    17101 atcccgagca tcaacgtttc taaacaccgt ctggacgttc agttcgacaa tgtggaagaa
    17161 gctattgcca tgtcggcaat gtacgcagct aaccacctga agggcgttac ggcgatcatt
    17221 accatgaccg aatcgggtcg taccgcgctg atgacctccc gtatcagctc tggtctacca
    17281 attttcgcca tgtcgcgcca tgaacgtacg ctgaacctga ctgctctcta tcgtggcgtt
    17341 acgccggtgc actttgatag cgctaatgac ggcgtagcag ctgccagcga agcggttaat
    17401 ctgctgcgcg ataaaggtta cttgatgtct ggtgacctag tgattgtcac ccagggcgac
    17461 gtgatgagta ccgtgggttc tactaatacc acgcgtattt taacggtaga gtaagtacgt
    17521 tgccggatgc ggcgaaaacg ccacatccgg cctatagttc aattatagtt caacagattt
    17581 cgaatattct gaagcaaact tgaacttatc atcaggcgaa ggcctctcct cgcgagaggc
    17641 ttttttattt gatgggataa agatctttgc gcttatacgg ctggatttcg cccggtttgc
    17701 gagttttcag caattttagt atccaggtgt attgttctgg tcgcggacca acaaaaatct
    17761 cgacttcttc attcatccgc cgctcaatcg tatgatcatc cgcctctaac agatcatcca
    17821 tcggtgggcg cacctgaatc gtcagacgat gcgtcttgcc atcataaatc ggaaacagcg
    17881 gtacaacgcg cgcacggcat actttcatca aacgaccaat cgcgggcaac gtcgctttat
    17941 aggtggcaaa gaaatccaca aattcgctgt gctctgggcc atgatcctga tcgggtaaat
    18001 aatatcccca gtacccctga cgtaccgact ggatgaatgg tttaataccg tcatttctcg
    18061 catgcagacg accgccaaag cgacgacgca ccgtgttcca gacataatca aaaaccgggt
    18121 tgccctgatt atggaacatc gctgccattt tctgcccttg cgaggccatc agcatggcag
    18181 gaatatcgac ggcccaaccg tgcggcacca gaaagataac tttctcgtta ttacgccgca
    18241 tctcttcgat gatctccagc ccttgccagc caacgcgcgg ctgaattttc tccggcccgc
    18301 gtattgccaa ctcagccatc attgccatcg cttgcggcgc ggtggcaaac atctcatcaa
    18361 caatcgcttc gcgttcagct tcactacgtt ctggaaagca gagcgacaga ttgattaacg
    18421 cacgacggcg tgagcttttt cccagtcgtc cggcaaaacg tcccagccgt gccagaatgg
    18481 gatcacggaa ctttggcggc gttaaagcga tacccgccat cgctgctaca cccagccatg
    18541 ctccccagta gcgcgggtgg cgaaaggatt tatcaaactc aggaatgtat tcgctattat
    18601 tttttttcgt ttccatgctt ttccagtttc ggataaggca aaaatcaatc tggtgatagt
    18661 gtagcggcgc aacttgcccc gcaccaaata aaaaaagccg gtactgactg cgtaccggct
    18721 gcgaatggat gttaattaat caaacagtag ctgcggcaca atctctttgg cctgcgccag
    18781 gaattcgcga cgatcggagc cggtcagccc ttcggtacgc ggcagttttg ccgtcagcgg
    18841 gtttacggcc tgctggttta tccatacttc atagtgcaga tgcggcccag ttgaacgtcc
    18901 ggtattaccg gaaagcgcga tacggtcgcc acgtttcacc ttctgtcccg gtttcaccag
    18961 aatcttgcgc aagtgcatat aacgcgtggt gtagctgcga ccatgacgaa tagccacata
    19021 ataacctgct gcgccactgc gtttggcaac caccacttca ccgtcaccca ctgaaagcac
    19081 tggcgtacct tgtggcatgg cgaaatcaac acctctgtgt ggtgcaacgc gacctgtcac
    19141 cggattagtt cgacgcgggt taaagttaga cgagatacgg aactgtttcg ccgtcgggaa
    19201 tcgcaagaat cctttcgcca gaccagtacc gttacgatcg tagaatttgc catcctcagc
    19261 gcggattgcg taataatctt taccttctga acgcaaacgt acgcccagca gctggctttg
    19321 ctcacgttta ccatcaagca tttctcgaga cattaatacc gcaaattcat cgcctttttt
    19381 cagtttgcgg aaatccattt gccactgcat ggctttaatc actgcgctca cttcggcgct
    19441 ggttaaaccg gcgtttctgg cgctggcaac aaagctccct ccgacggtac ctttcagcag
    19501 attgttaacc cactctcctt gctgcatttc gctggtcatt ttaaaaccgt tagcggcagt
    19561 acggtcatag gttcgggttt cacgacgaga cacttcccag gtgaggcgct gcagttcgcc
    19621 gtccgcggtt aatgtccagg agagttgttg accgattttc aggttacgca attctttgtc
    19681 ggcagcagcg agttgggtga tatcacccat atcaatacca tactgattga gaatgctgct
    19741 tagcgtatcg ccagtggaaa caacatattc atgcacgccc gcttcaccgg cgattttgtc
    19801 atccagttcg tcctggggaa tggcttcatc ttcttgtgca gcttgatcaa tcggctcact
    19861 ggcttcaggt aagagcgaac gaatttcgtt ctgttccagc tcaatggttt tgacaattgg
    19921 cgtggcatca cggtgataaa catagggccg ccagacagcg acggccagag taagaacggt
    19981 gagcgacccc aacataacgc ggtgtggtcg cggtaaatta ttaaacgcca gggcgacaga
    20041 gcgggctatc tgttgcacgt aatcacttcc tcattaatct cctttcaggc agctcgcata
    20101 ctggttggct aattgactca ggaattctga atagcttgtt ttacccagtt tgatattcgt
    20161 ccccagggga tccaacgttc ccatacgaac ggatgtccct cgtgcgacgc tctcaacgac
    20221 cgctggcctg aactgtggct cagcaaaaac gcaggttgct ttttgctcaa ccaactgtgt
    20281 tcttatttca tgtaaacgct gcgcgccagg ttgaatctca gggttaacgg taaaatgacc
    20341 aagcggtgtc agtccgaact gtttttcgaa atagccgtaa gcatcgtgaa aaacgaaata
    20401 acctttcccc ttgagcggcg cgagctcgtt accaacctgc gtttcggttg aggccaattg
    20461 tgcctcaaaa tccttcaggt tggcgtcaag tttggctcga ctttgcggca taagttccac
    20521 taattttcca tggattgcaa ccgctgtagc ccgcgctatc tctggggaaa gccaaagatg
    20581 catgttgaaa tcgccgtgat ggtgatcttc gtcacttttt tccgcgtggt cgtgatcatc
    20641 atcatcgccg tgaatacttt tcatcagtag cggtttcaca tcttcaagct gcgcaatcgt
    20701 tacctgcttc gcttctggta atttgcttac cggtttttgc ataaacgctt ccatctccgg
    20761 gccaacccaa acgactaagt ccgcgttctg taagcgtttt acatccgatg gacgcagtga
    20821 ataatcatgt tctgaagcgc cgtcaggcag taaaacctcc gtttctgtta caccatcagc
    20881 aatggcagaa gcgatgaacc caacgggttt aagcgaagcg acaacggcgg catctgcggc
    20941 ctgtgttgca cctccccaga gagcggcgga taatgctgcg aaaagaagcg tttttttatg
    21001 taacataatg cgaccaatca tcgtaatgaa tatgagaagt gtgatattat aacatttcat
    21061 gactactgca agactaaaat taacatgaca agtctggttt ccctggaaaa tgtctcggtt
    21121 tcttttggcc aacgccgcgt cctctctgat gtgtcgctgg aacttaaacc tggaaaaatt
    21181 ttgactttac ttgggccaaa tggcgcaggt aagtcgacac tggtacgggt agtgctcggg
    21241 ctggtaacac ccgatgaagg ggttatcaag cgcaacggaa aactgcgcat cggctatgta
    21301 ccgcagaagc tgtatctcga caccacgttg ccactgaccg taaaccgttt tttacgctta
    21361 cgccctggta cacataaaga agatattttg cctgcactga aacgtgtcca ggccgggcac
    21421 ctgattaacg caccgatgca aaagctctct ggtggcgaaa cgcagcgtgt actattagcg
    21481 cgagcattgt taaattgccc gcaattatta gtgctggatg aacccactca aggcgtggat
    21541 gtaaatggcc aagtggcgtt atatgacctt attgaccagt tgcgtcgtga actggattgt
    21601 ggcgttttaa tggtttctca cgatctgcat ctggtgatgg caaaaaccga tgaagtgctg
    21661 tgcctgaatc accacatttg ttgttccggc acaccggaag ttgtttccct gcatccggag
    21721 tttatttcaa tgtttggtcc tcgtggtgct gaacaactgg gtatctatcg ccatcatcat
    21781 aatcatcgtc acgatttaca gggacgaatt gttttgcgtc ggggaaatga tcgctcatga
    21841 ttgaattatt atttcccggt tggttagccg ggatcatgct cgcctgtgcc gcgggtccgc
    21901 tgggttcgtt tgtagtctgg cgtcgtatgt cttatttcgg tgatacgctg gctcatgcct
    21961 cattacttgg tgtcgcgttt ggtttgttgc tggacgtgaa tccattctat gcggtgattg
    22021 ccgttacgct gctgctggcg ggcggtctgg tatggctgga gaagcgtcca cagctggcga
    22081 tcgacacgtt attagggatt atggcgcaca gtgccctgtc gctgggccta gtggtcgtta
    22141 gtctgatgtc taacattcgt gttgatttga tggcttacct gttcggtgat ttactggcag
    22201 tgacgccaga agatctcatc tctattgcga ttggcgtggt catcgtggtg gctattttgt
    22261 tctggcaatg gcgcaatttg ctgtcgatga cgattagccc ggatctggcg tttgttgatg
    22321 gtgtgaaatt acagcaagtg aaattgttgt tgatgctggt gacggcattg acgattggtg
    22381 tagcgatgaa atttgtcggt gcgttgatta ttacttcgtt gctgattatt cctgcagcta
    22441 ctgcgcgtcg ctttgcccgt acgccggaac agatggctgg tgtcgctgtt ttggtgggga
    22501 tggtggcagt aactggcggt ttaacctttt ccgcgtttta cgatacgccg gcgggtccgt
    22561 cggtggtgct atgtgcggca ctgttattta ttatcagtat gatgaaaaag caggccagct
    22621 aatctatcgc tgaacacatt tgtcggatgc ggcgcgagcg ccttatccga cctacggttc
    22681 gctatctctg gctggcctga taagacgcga acagtgtcgc atcagacaca ctgccagtgt
    22741 cggatgcggc gcgagcgacc aatccgactt acggcatttc tggcggcgtt atgccaaagt
    22801 gattccacgc ccgcgtcgtc gccatacgcc cgcgcggtgt acgctgcaaa aagccttgct
    22861 gaatcaaata aggttccagt acatcttcaa tggtttcacg ttcttcgcca atggctgccg
    22921 ccaggttatc cagacctacc ggcccaccaa agaacttatc gattaccgcc agcaacaatt
    22981 tgcggtccat ataatcgaaa ccttcagcat cgacattcaa catatccagc gcctgagcag
    23041 cgatatctgc cgagatggtg ccatcgtgct tcacttcggc gaaatcacgc actcgacgca
    23101 gcagacggtt ggcaatacgt ggcgtaccgc gagcgcgacg agctacttcc tgcgcaccgt
    23161 catcactcat ctcaagcccc ataaagcgtg cactgcgact gacgatatat tgcagatccg
    23221 gcacctgata aaactccaga cgttgcacaa taccaaaacg gtcgcgcaac ggtgatgtca
    23281 gcgaacctgc gcgcgtggtt gcgccaatca gggtaaacgg cggcaaatca attttaatgg
    23341 agcgtgccgc cggaccttca ccaatcatga tatccagttg gtagtcttcc attgccgggt
    23401 acagcacttc ttcaacaact ggcgataaac ggtggatctc atcaataaac agcacgtcat
    23461 gcggttcaag gttagtgagc atcgcagcca aatcgcccgc cttttccagc accggaccag
    23521 aagtcgtgcg taaattaacg cccatttcat tggcgacaat gttggcaagc gtagttttac
    23581 ccaaccccgg aggaccaaaa atcaacaaat gatcgagggc atcgccgcgc agtttcgctg
    23641 ctttgatgaa aatctccatc tgtgaacgaa cctgcggctg accaacatac tcttccagta
    23701 atttagggcg aatggcgcga tctgctacat cttccggcaa agtggtaccg gcagaaatca
    23761 gacggtctgc ttcaatcatc ctttacctca taacgcggcg cgtagggctt cgcgaattaa
    23821 tgtttcactg ctggcgtcag ggcgagcgat tttgctcacc atgcggcttg cttcttgtgg
    23881 tttatagcct agcgccacca gcgcggcaac tgcttcttgt tcagcatcgt cggtcgccgg
    23941 gctggcagga gacgtgagta ccaggtcggc ggctggcgta aagagatcgc catgcaaacc
    24001 tttaaatcgg tctttcattt caacaatcaa gcgttcggcg gtttttttgc caatacccgg
    24061 cagtttcacc agtgccccca cttcttcacg ctcaacggca ttaacgaact gctgcgctga
    24121 cattccggag aggatcgcca gggccaactt cgggccgacg ccgttggttt tgatcaactc
    24181 tttgaacaac gtgcgttctt gtttattgtt aaaaccgtac agcagttgcg cgtcttcacg
    24241 caccacaaag tgggtgaaaa cgatcgcttc ctgacccgct tcagggagtt cataaaaaca
    24301 ggtcatcggc atatgcactt catagcctac gccgcccact tcaattaaca ccagcggggg
    24361 ttgtttttca atgatgatgc ctctgagtct gcctatcaca tgacgctcct gcgtaatgaa
    24421 tcaaagataa tgctgtatga taaaaaaatg ctggatagat atccagcgta ggatgaagaa
    24481 aacttgcgag gtgtcccgat gatctgaaaa atggcacagt ataatttatt caacaggtta
    24541 tattggaagc aaatatttga atattacata ttcagtgaag aaatgcgtaa taaaaatata
    24601 cattgcgact cctgaaaaaa taattttttt atgctattac gtatattcgt atctatttca
    24661 atggaatgac aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt
    24721 tacatgtata ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa
    24781 tcacgtcggg atcattatcg gtcataacgg tgaagacttc ctggttgcag aaagccgggt
    24841 tcccctttca a
//
LOCUS       DAJVQH010000050        24605 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJVQH010000050 DAJVQH010000000
VERSION     DAJVQH010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.050"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..691)
                     /gene="fdhD"
                     /locus_tag="OQM87_002099"
     CDS             complement(<1..691)
                     /gene="fdhD"
                     /locus_tag="OQM87_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCS1508237.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAM"
     gene            885..3935
                     /gene="fdnG"
                     /locus_tag="OQM87_002100"
     CDS             885..3935
                     /gene="fdnG"
                     /locus_tag="OQM87_002100"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1470..1472,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1508238.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMFSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGITLTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            3948..4850
                     /gene="fdoH"
                     /locus_tag="OQM87_002101"
     CDS             3948..4850
                     /gene="fdoH"
                     /locus_tag="OQM87_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCS1508239.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            4847..5482
                     /gene="fdoI"
                     /locus_tag="OQM87_002102"
     CDS             4847..5482
                     /gene="fdoI"
                     /locus_tag="OQM87_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1508240.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5479..6408
                     /gene="fdhE"
                     /locus_tag="OQM87_002103"
     CDS             5479..6408
                     /gene="fdhE"
                     /locus_tag="OQM87_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCS1508241.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6738..6980)
                     /locus_tag="OQM87_002104"
     CDS             complement(6738..6980)
                     /locus_tag="OQM87_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508242.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7198..7416)
                     /locus_tag="OQM87_002105"
     CDS             complement(7198..7416)
                     /locus_tag="OQM87_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCS1508243.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(8095..9228)
                     /locus_tag="OQM87_002106"
     CDS             complement(8095..9228)
                     /locus_tag="OQM87_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508244.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKP
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATVVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKNLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            complement(9398..9736)
                     /locus_tag="OQM87_002107"
     CDS             complement(9398..9736)
                     /locus_tag="OQM87_002107"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508245.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            complement(10326..11267)
                     /gene="fabY"
                     /locus_tag="OQM87_002108"
                     /gene_synonym="yiiD"
     CDS             complement(10326..11267)
                     /gene="fabY"
                     /locus_tag="OQM87_002108"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCS1508246.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(11312..11749)
                     /gene="dtd"
                     /locus_tag="OQM87_002109"
     CDS             complement(11312..11749)
                     /gene="dtd"
                     /locus_tag="OQM87_002109"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCS1508247.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11746..12618)
                     /locus_tag="OQM87_002110"
     CDS             complement(11746..12618)
                     /locus_tag="OQM87_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCS1508248.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12612..13211)
                     /gene="yihX"
                     /locus_tag="OQM87_002111"
     CDS             complement(12612..13211)
                     /gene="yihX"
                     /locus_tag="OQM87_002111"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCS1508249.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(13310..14094)
                     /locus_tag="OQM87_002112"
                     /pseudo
     CDS             complement(13310..14094)
                     /locus_tag="OQM87_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(14128..15024)
                     /gene="yihV"
                     /locus_tag="OQM87_002113"
     CDS             complement(14128..15024)
                     /gene="yihV"
                     /locus_tag="OQM87_002113"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCS1508250.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGVKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            15957..16847
                     /gene="yihT"
                     /locus_tag="OQM87_002114"
     CDS             15957..16847
                     /gene="yihT"
                     /locus_tag="OQM87_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCS1508251.1"
                     /translation="MPLGAASREVYSQARASGGFAMLAVDQREAMRMMFAAAGAPAPV
                     ADSVLTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNG
                     IPVDSVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNGLCHSHGLVS
                     IIEPVVRPPRRGDKFDREQAVIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRL
                     NDHINMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDV
                     CAPKLQQLGDIVDEMMAKRR"
     gene            16864..18105
                     /gene="yihS"
                     /locus_tag="OQM87_002115"
     CDS             16864..18105
                     /gene="yihS"
                     /locus_tag="OQM87_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCS1508252.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            18243..19145
                     /locus_tag="OQM87_002116"
     CDS             18243..19145
                     /locus_tag="OQM87_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCS1508253.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            19344..21380
                     /gene="yihQ"
                     /locus_tag="OQM87_002117"
                     /pseudo
     CDS             19344..21380
                     /gene="yihQ"
                     /locus_tag="OQM87_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            21426..22811
                     /locus_tag="OQM87_002118"
     CDS             21426..22811
                     /locus_tag="OQM87_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1508254.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            22854..24257
                     /locus_tag="OQM87_002119"
     CDS             22854..24257
                     /locus_tag="OQM87_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1508255.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            24325..>24605
                     /gene="ompL"
                     /locus_tag="OQM87_002120"
     CDS             24325..>24605
                     /gene="ompL"
                     /locus_tag="OQM87_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCS1508256.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGG"
BASE COUNT         6079 a         6329 c         6053 g         6144 t
ORIGIN      
        1 acatcgccga cttttgcacc atttcataac tggcacgact ggaaaccagt accgcacctt
       61 gctgccagct ttccccttct tgtgaccggc ggcctaacag tttgtccagc gccacatggc
      121 gacccacgtc ttcatgcccg ccgaccagtt cgccagatgg caacatccag gcagcggcgt
      181 gagtacaacc agtcagttgc cccactggct gaaaatcgtt gagatgacgt aatgcatcat
      241 ccagtttgtt gagatcaaac gtctgggtga acggtagcgg ctgcaccggt tttccgatgt
      301 cattaagttg ctccacgccg catacgccac agcccgtacg tcccgccagc gcccggcggc
      361 gctccttcaa ccccataaag cggcggctgg aaagctcaat ttgtacttca agaccattac
      421 aggaaggaac gacatccatg ccgaagatat cgcgcggact ttctattatc ccctcggaaa
      481 gcgaaaaacc gagcgcaaag tactcaaggt ctttgggcga cgccatcatc accacatgcg
      541 aaatgccgtt gtagaccagc gcaacgggaa cttcttccgc gacctcatct aaccgtgggt
      601 gttgtaaatc atcacgccgc cataactcaa tttgacgaac acctgtgata tttgtcacat
      661 tttcgatttc ttttcgctgt gtttttttca ctttctctta accagataag aacacacaca
      721 ccaacattat ggtattctgt tacaaaccct tcctagatgg agggaaattg agccaattct
      781 ggacctttgc ggccccttcc gcaaagaaaa ataactccca ctccctgcac acgcagcaag
      841 cgaatgtaaa tgggacgtga caatgtcgaa acaaggagca atccatgcag gtcagcagaa
      901 ggcagttctt taagatctgc gctggcggta tggcaggcac cacggcagcg gcactgggtt
      961 ttgcacccag cgtagcactc gcggaaaccc ggcagtataa actgctgcgc acccgcgaaa
     1021 cccgtaatac ctgcacctat tgttccgtag gctgtgggct gttgatgttc agcctcggtg
     1081 acggagcaaa aaacgccaaa gcatctatct tccatatcga aggcgatccg gatcacccgg
     1141 tcaaccgcgg tgcactttgt ccgaaaggcg ctggcctggt ggatttcatc cactccgaaa
     1201 gccgtctgaa gtttccggaa taccgtgcgc caggttctga taaatggcag caaatcagtt
     1261 gggaagaggc gtttgatcgc atcgccaaac tgatgaaaga agaccgcgat gctaactaca
     1321 ttgcgcaaaa cgccgaaggc gtgactgtta accgctggct ctccaccggg atgctgtgtg
     1381 cttccgcgtc gagtaacgaa accggctatt taacgcaaaa attctcccgc gcgctgggta
     1441 tgctcgcggt cgacaaccag gcgcgtgtct gacacggacc aacggtagca agtcttgctc
     1501 caacatttgg tcgcggtgcg atgaccaacc actgggtcga catcaagaac gccaacctcg
     1561 tcgtggtgat gggcggtaac gccgctgaag ctcacccggt cgggttccgc tgggcgatgg
     1621 aagccaaaat tcacaacggc gcgaagctga ttgtgatcga tcctcgcttt acgcgtacgg
     1681 cggcggtagc tgactactat gcccctattc gttccggtac tgacattgct ttcctgtcag
     1741 gcgtattgct gtacctgctg aacaatgaaa aattcaacca cgaatacacc gaagcctata
     1801 ccaacgccag cctgatcgtg cgtgaggatt acggctttga agatggcctg ttcaccggct
     1861 acgacgcgga aaaacgcaag tacgataaat cctcctggac ttatgaactg gacgaaaacg
     1921 gcttcgccaa acgcgatacc acgctgcaac atccgcgctg cgtgtggaac ttgctgaaac
     1981 agcacgtttc ccgctacacg ccagatgtgg ttgaaaacat ctgtggtacg ccaaaagacg
     2041 cgttcctgaa agtctgcgaa tacatcgcag aaaccagtgc tcacgataaa actgcctcgt
     2101 tcctgtatgc cctcggctgg acgcaacact ccgttggtgc gcaaaacatt cgtacgatgg
     2161 cgatgatcca gctgctgctc ggcaatatgg ggatggccgg cggcggcgtt aacgccctgc
     2221 gcggtcactc caatattcag gggctgacgg acctggggct actgtcgcag agtctgccag
     2281 gttacatgac gctgccaagc gagaagcaga ccgatctgca aacctacctt accgccaaca
     2341 cgccaaaacc gctgctggaa ggccaggtaa actactgggg caactacccg aaattcttcg
     2401 tctctatgat gaaggccttc tttggtgata aagcgacggc ggaaaatagc tggggctttg
     2461 actggttgcc gaagtgggat aaaggctacg acgtcctgca atacttcgag atgatgaaag
     2521 agggcaaggt caatggctat atctgccagg gctttaaccc tgttgcctca ttcccgaaca
     2581 aaaacaaagt gatcggttgt ctgtcgaaac tgaagttcct cgtcaccatc gacccactga
     2641 acactgaaac ctctaacttc tggcagaacc acggtgagct gaacgaagtt gactcgtcga
     2701 agatccagac cgaagtgttc cgtctgccat cgacctgctt cgcagaagag aacggttcaa
     2761 tcgttaactc aggtcgctgg ttgcagtggc actggaaagg tgcggacgcc ccggggatta
     2821 cgctgactga cggtgagatc ctctccggta tcttcctgcg cttgcgcaag atgtatgccg
     2881 aacagggtgg cgcgaacccg gatcaggtgc tgaacatgac ctggaactac gccattccgc
     2941 atgagccgaa gtcggaagaa gtagcgatgg agagcaacgg taaggcgctg gccgatatta
     3001 ccgatccggc aaccggtgcg gttatcgtca agaaaggcca acaacttagc tcgttcgccc
     3061 aattgcgtga tgacggtaca acctcctgtg gctgctggat tttcgccggt agctggacgc
     3121 cggaaggcaa ccagatggcg cgtcgtgata acgccgatcc gtctggcctc ggtaacacgc
     3181 tgggctgggc atgggcatgg ccgcttaacc gccgcattct gtataaccgc gcctccgcag
     3241 atccgcaggg taacccgtgg gatccgaagc gtcagttgct gaaatgggac ggcactaagt
     3301 ggaccggctg ggatattccg gactacagcg cagcgcctcc gggtagcggc gtcgggccgt
     3361 ttatcatgca gcaggaaggc atggggcgtc tgtttgccct cgataagatg gcggaaggtc
     3421 cgttcccgga acactacgag ccgtttgaaa cgccgctggg aactaacccg ctgcatccaa
     3481 acgttatctc gaacccggct gcacgtatct ttaaagacga cgccgaagca ttgggtaaag
     3541 ccgataagtt cccgtatgtc ggaaccacct atcgtctgac cgagcacttc cactactgga
     3601 ccaaacacgc gctgttgaac gcgattttgc agccagagca gtttgtggaa atcggggagt
     3661 cgctggcgaa taaacttggc attgcccagg gcgataccgt gaaagtctcc tccaaccgtg
     3721 gctatatcaa agccaaagcg gtggtgacca aacgtattcg cacgctgaaa gcgaacggca
     3781 aagatatcga taccatcggt attccgattc actggggcta tgaaggcgta gctaaaaaag
     3841 gctttattgc caatacttta acgccattcg ttggtgatgc gaacacgcag acgccggagt
     3901 ttaagtcctt ccttgtgaat gtggaaaagg tgtaacggag acgacatatg gcttatcaat
     3961 ctcaagatat cattcgtcgt tccgcgacta acggtctgac ccccgcgcct caggcgcggg
     4021 acttccagga agaagtggcg aaactcatcg acgttaccac ctgtatcggc tgtaaagcct
     4081 gtcaggtggc gtgttcagag tggaacgaca ttcgcgatac cgtcggcaat aacattgggg
     4141 tgtacgacaa ccccaatgat ttaagcgcca aatcgtggac ggtaatgcgc ttctcggaag
     4201 tggagcagaa cgacaaactg gaatggctga tccgcaaaga cggctgtatg cactgttccg
     4261 atccaggctg cctgaaagcg tgcccggcgg aaggtgcaat tattcagtat gccaacggta
     4321 tcgtcgactt ccagtccgag cagtgcattg gctgcggtta ttgcattgcg ggctgtccgt
     4381 tcgacattcc gcgcctcaac ccggaagaca accgcgtcta caaatgtacg ctgtgcgttg
     4441 accgcgtggt ggttgggcaa gaaccagcct gcgtgaagac ctgcccaacg ggcgcgattc
     4501 acttcggtac gaaagagtcg atgaaaacgc tggcgagcga gcgcgttgct gagctgaaaa
     4561 cccgcggtta cgacaatgcg ggtctgtacg atccggcagg cgtcggtggt acacacgtca
     4621 tgtacgtgct gcaccatgct gacaagccaa atctgtatca tggcttgccg gagaacccgg
     4681 aaatcagcga aaccgtgaaa ttctggaaag gcatctggaa accgctcgcg gctgttggct
     4741 ttgcggctac ctttgcggcc agtatcttcc actacgtggg tgtcggtccg aaccgtgcgg
     4801 atgaggaaga gaataatctg cacgaagaga aagacgagga gcgcaaatga aacgacgtga
     4861 caccatcgtg cgctacaccg cgccggaacg tatcaaccac tggatcaccg ccttctgctt
     4921 catcctggcg gcggtgagcg ggctgggctt tttgttcccg tccttcaact ggttgatgca
     4981 aatcatgggc acaccgcagc tggcgcgaat tctgcacccg tttgtcggcg tggttatgtt
     5041 tgcctcgttc atcatcatgt ttttccgtta ctggcatcaa aacctaatca atcgggatga
     5101 tatcttttgg gcgaagaata ttcgtaagat cgtcgtcaac gaggaagtag gtgacaccgg
     5161 gcgttataac ttcggtcaga aatgcgtttt ctgggcggcg attattttcc tggttctgct
     5221 gctggtgagc ggcgtgatta tctggcgtcc ttattttgcg cctgctttct caatcccggt
     5281 gatccgattc gcgttaatgc tgcattcatt tgccgcagtg gcgttaattg tggttatcat
     5341 ggtgcatatc tacgccgccc tttgggtgaa aggcacgatt accgccatgg tggaaggatg
     5401 ggttaccagc gcctgggcga agaaacatca cccgcgctgg taccgtgagg tccgcaagac
     5461 aacggaaaag aaagctgaat gagtattcgc ataatcccgc aagatgagct gggttcgagc
     5521 gagaaacgta cggcgaatat gattccgccg ttattgttcc ctcggctcaa gaatttatac
     5581 aaccgccgcg ccgagcgtct gcgcgagctg gcagaaaata atccgctggg tgattacctg
     5641 cgctttgctg cgcttatcgc ccacgcccag gaagtggtgc tgtacgacca tccgctggag
     5701 atggatctga ccgcgcgcat taaagaagcc agcgcacagg gcaagccccc gctggatatt
     5761 cacgttctgc cgcgtgataa gcactggcaa aagctgctga tggcgctaat tgctgagctg
     5821 aaacctgaaa tgagcggccc ggcgctggca gtgattgaga atctggagaa ggcatcgact
     5881 caggagctgg aagatatggc cagcgcgctg tttgcctctg atttctcgtc cgtcagcagc
     5941 gataaagcgc cgtttatctg ggctgcgctg tcgctctact gggcgcagat ggccaatctg
     6001 atccccggca aagcccgcgc tgaatacggc gaacaacgtc aatattgccc ggtatgtggc
     6061 tctatgccgg tgtccagcat ggtgcaaatt ggcaccactc agggtctgcg ttacctgcac
     6121 tgcaacctgt gtgaaaccga atggcacgta gtgcgcgtaa aatgcagcaa ctgcgagcag
     6181 agcggcaaac tgcattactg gtcgctggat gacgaacagg ccgcgattaa agccgaaagc
     6241 tgcgatgact gcggcactta cctgaagatt ctttatcagg aaaaagaacc gaaagttgaa
     6301 gccgtggcag atgacctcgc ctctctggta ctggacgcgc gaatggagca agaaggctac
     6361 gcccgtagtt ccattaaccc gttcctgttt ccgggtgaag gagagtaatc tctgacgttt
     6421 tccgggtggc gcaggtcgcc cggattacag ctcatagggt gtgacgacac catctctcgt
     6481 attccgcgtt acccattcaa gatcatgccg caattgtctg tttgcctcgt tcttccctcc
     6541 aacacatcga taaggttatg ggtatgtcac tgatgacaag cgtatgattg cctgatgcgc
     6601 tgcgcttatc aggcctacca ataaacttca atatattgaa tttgcatgac tttgtaggcc
     6661 ggataaggcg ttcacgccgc atccggcatg aacaacgcgc acgttgtcag caatctgatt
     6721 gacatcattt tttcccatta ccattcctcg cgcagcttac gctgatattc gacaccatct
     6781 tcctcacacc cttgccagat gccaggaaca ctggttcttg ctgtttgcga ttgatttatc
     6841 aggtattgat ctaccgcttc ccttaatagg tccgcacgag gaagattacg ctgcacctcg
     6901 agatcatcaa gttgcttaat cacctcattc gataaatcga gtaaaattct gcccatatct
     6961 atacctgcca gagagttcat aaatatctcg ccaatatcat ttttgaatct atggagagaa
     7021 aagtaccctt gccgaatctt taaagaaagc tcatttacgc atcatttttt atttttgcga
     7081 ggcgttttcc agatacgatg taacagttgc agttattata agttcaagta aaacagcctt
     7141 gtattaatac cagaataagg gcatctgttt tttatattca agaatgaaaa atttttttca
     7201 ttccttatgc tccttacact gatcatcaac accaactacc tccccctttt gcctgcctcc
     7261 ctttgcttta cgcaccggaa attgctgccg ctccagatat tgctcaaccg cttcgcgtaa
     7321 aattgcagat cgtgaaacct tacgccagcc ccgaagcttg ttcagccgtt taattgcctc
     7381 ttctgataaa tgaagaaaaa ccgtattcat cgccattacc accaccttac atatattact
     7441 cattaatgta tgtgcgaaaa ttacgccgaa aaagcattta aaaaaatgcg gatttttgca
     7501 tgtcattttg tttttgcgag gcgttttcca gaaataataa gagcgttgca gttgtttcat
     7561 ttattactca aaaatggttt agttatcgcc caggatgagt tatgggtgct atgaatatat
     7621 tatattcacg gatatttatt tgcataatct gaatggtgta gccgaaaaac gtcagcatcc
     7681 taacagcacc aggatttaag gtgaaattaa tctttcatca cagaccggtc agtactgcca
     7741 ccaaccggtc tttacttgct catcgttata attttgttgg taacagttcg ggaaacaagg
     7801 aattccttcc ggaacacccc cggcatcatc cgatagcatt gacgtcaatc atcagcgcga
     7861 cgatgagttt ttttaatcgc atcatagggt ggcgacgttc agcacgatta cgggtcatag
     7921 caaaactcct gagcaggcgg ccactatgcc gctttcactg gagttctgaa tactggtact
     7981 ttctgtatgg cttcgccatg cccctcgcgg ctaagtacct gcacacatct ctctttgtat
     8041 gtcatttttg caatgcattg tccgaattct gataatccta atgagtttta ctcgtcagac
     8101 tcccatccct acggtatacg agttatattc atgaatgaaa tcgacatttt taaaaaacga
     8161 atcttgtaaa acatcagtgt tgatattacc ttgcaatcca acattgaatt tgttagagac
     8221 atgaagaact cgaatatcaa aaaggttttt taacatcatt aattcttcgg gtgttaatga
     8281 atcgcaacta aaatatccct gttgatcaaa aagattttct atttctccac tatcggcgag
     8341 gcgtttttca aggagtgttt gcaacatcga gttgccaagg acaatggata tcgtgcgatc
     8401 tttcgcagat ttacacccca tcacaacggc aacattcgta tccccctcaa gagtttcatt
     8461 tatcgccgtc gctaaaatgg tggtcaaaca cgcaaacgga actgtatttc tttcgtgatt
     8521 taatttatct accagttcat tccatttatc aaaagccggg ctatatttgt tgcgcacaga
     8581 aatatccgac tggttaaaca attcagccat gcgatttttt atcatctcga tatccggcga
     8641 accagaaaga ctttgcgcat aatacggtcc tgatgatgtt gcatacatta atgccaagcc
     8701 cacaactgtc gccatgatgg gcgcagccac ggttttggca agggcgacgc tgcgatggct
     8761 atgcgcttca caggctaaag gaacatattc cactgactgc ggtaattcct tacatgcctc
     8821 cctcatcatt gcggtatgct ttacaaacaa cggtttttca ctgcccaaaa aaactttctg
     8881 cttaattgca ctaagaaaac gcaaatcaaa aataattgtt tttggtttat tattttttgg
     8941 cacatttcct attatcccgc taagaacctt ccttgctgca tggagatttt tttgcacagc
     9001 acataacaca attgatgaag ccttctccac tgcattattg attttaattt taaaatccgc
     9061 atgatatacg ttgacgggca atgcaagtat atctggacct acatcttttg ctgagtattt
     9121 attggtgatc gacggacgtg aacccacgag aaaacttagc tgcaaattat gctctatatt
     9181 atcagctgtt tttgtggtgt ggttaaacat agagatgcca cctttcattc tttcacacat
     9241 atcctcaact ttttcacttc gttcttgtgg caatttggct ttaacattaa tttgatgatt
     9301 tttacccgtg cattccttaa ctacaagggg aatgttatct aaagtgccat acaggctaat
     9361 aacgtcacca ggatttttat catgtaagta agtttgatta atacttgaag cattcacaaa
     9421 gagggtcaat attataactg tttataactt cacctacatc aagtgtgtag tgcttactct
     9481 ggagactgcc atccttattt aattgtatat caatcaatat atccccggac tttgtcacca
     9541 tatctttgcc ataaaccatt tgcatagcca tttcgaggag atttttatct aactgattgc
     9601 gggcgttgag taagcgatta ccaaataatt tatatactgt tcccgtcatt gccagcgctg
     9661 cgctttctgg tactttgcgt ttccacagtg aggtttctct atttttaaac aggtcatatt
     9721 cattattact cgacaaacca tgcttcagtt cgttaacaca atcgcacagt tgctcctcac
     9781 cttccatttt aaggcaaagt gaaaattgat ttaatggcgt ttacttcggt gatttattac
     9841 tcattagttg tttttctaaa ttaacaacag aataactttc ttgtttaatg agattaatat
     9901 tggcaaccat tatagcctcc ttcccacggt ttaagagcaa acatacaaca taatgattca
     9961 taacatacag agcagtaaat gaatgatact gacaatgtat gccagcccat tatctttgca
    10021 taatgtttgt gagctataga ttttaaaaat atttatgaaa aaagtatggc accagagggg
    10081 gttttgacta tcaaaaatcg ctcaaacctg aactaagatg atgagttaag cattatctgc
    10141 aataattgcc actcagctct acaaataata tttaaatgct cctgaacagt gatgtagcaa
    10201 gacacgtatg gtgccaacga agaataaaaa tttataaagt tcttattcaa tataaccgga
    10261 atagtcaggg actgatgtaa atgaccggaa tgaatgagag aggcagcacc gcccctctcc
    10321 ataagctact cttcttcgtt cccgccctct tcatacgggc caaatggctt cgcgggcaga
    10381 acgatatacg tgccttcaaa cactgcaccc ggcgtctcgt cgccaaagat ttcgacctgc
    10441 atctgcaccc gtgcttttcg tccgcgcgcc agacggtcga gatcgccgct taaggcacca
    10501 aggtcggcta ccgcatgggg tttaccgcta atcggtttgc tgtagcggat atgcgcatcc
    10561 gccagaataa tcgttccgcc gaggtggcgt tcacgcagca tcagccagat aagcccccaa
    10621 ccggtgagcg tcgccagcga gaataaactc ccggcaaaca gcgtatggtg cggattctga
    10681 ttgccggttt ccggcatggt ggtgataaat ttttgcccgg tatattgttg aatacgcacg
    10741 cccatttttt cactaagcgg gatatgttcg taccacgcct gttgcagctg cgcgcaccag
    10801 tcgccgcgat gcaaaatatc atccagagtg gcgacgggct taatcatcaa aaaatggcga
    10861 atcggcgtgg tggttggcgt ggtgatttct ccctgattaa caaaccccag cttggcgaaa
    10921 aactccaccg cgtcttcacg ggcgctacag gtcacgcgct taacgccttc ctgacgcgcc
    10981 accgactcca gggtcatcgc catcagcgtt cctaaccctt tgtcctgcac gtccgggtga
    11041 acggccataa agcgaatgga cgcttcattg tcggcattaa tatacaatcg gcctaccgcc
    11101 accagattac cctgctcgtc gacgaccatc tggtgatgcg ccatcgcatc ccacgcgtcg
    11161 cgttccgaac cttttggttg atgcaggggc ttacgcaaca tttcccagcg aaactgatag
    11221 taacgctcta actcttcttc tgtttgtgga acctgaaggt gatacatagc gatactctct
    11281 cttgtttccc gtgacaaccc tggaagctgg ctcatacctg caaccagaat gtcacggggc
    11341 catcattgac cagcgatacc tgcatatcgg cagcgaagcg tcctgtttgc gtgttcatct
    11401 cttgctggcg gcagcgttcg acgaaatagt catataacgc ctctgcgcga tccggtgatg
    11461 cgcctttgga gaaacttggg cgcatccccc gttcggtatc tgcggcgagg gtaaactggg
    11521 aaaccaccag tacactgccg cccgcctgtt gcacgttgag attcatcttg ccttcggcat
    11581 cgctaaagat gcggtagccg agcacacgct cgcacagacg gtttgctttc tgttcgtcgt
    11641 catccttttc gacacccaat aacaccaaaa gtcccgcgcc aatttcgccc gtcacttctc
    11701 cctccacggt gacgctggca cgggttacgc gttgaattaa tgcaatcatg gttcgtcgtc
    11761 ttcttcttgt tcagctgctt gttttagttt gcggtattcc ccgagagtga cagtaatttc
    11821 cgcgccaagc aagacgatac accatgtcca gtagacccaa acaaagagaa tggggatcac
    11881 cgccagcaca ccgtaaatga gctgatatga cgggaacatg gtgatataaa gcgcgaaacc
    11941 tttctttcct gcttcgaaca ggagtgcggc gacaaacgcg ccgacaatcg cgtcgcggtt
    12001 aggtacgcgg atggtaggaa caatgctgta cagcaaccag aaggagatcc acgacaacag
    12061 caacggaaaa atacgcagca cgttgtcgat gacagtattg agatcgctcg cccagcgcag
    12121 ggagagcaaa taggaactga tcgccagact ggcccctgcc agcagcggcc ccagcgttaa
    12181 aatcatccag tacacggcga acgagtaaat ttttggtcgc gctcgtttac tgcgccagat
    12241 ggtattcaac gcgctatcga tggagtacat caacaataac gcggtgacga tcagcccgca
    12301 cgccccaacg gcggtcatct tgttggaatt agcaacaaat tgttcgatat accgctgaat
    12361 aacatcgcca gtagcaggca gaaagttggc aaaaataaag tgacgtaact gaatgctgac
    12421 gtcggaaaac atgggaaaag cggcgaaaag cgcaaaaaca acggcaacca gcggcactaa
    12481 tgagagcaac gacacatagg caaggttacc tgccagggtt gtcatgttgt cctcatcaat
    12541 acgttgccag agtagtttta gccaggccca tagtggacgg gtacggtgcc tggctttgtc
    12601 ctgaatggtt tttagcataa caccttcgcg aaatagtccg ggatggtggt tttatctttc
    12661 accagaatac tggtaatgcc tagctgatta gctccttcta tattatcggc gttatcgtcg
    12721 aaaaagaccg tatcgctggg tgaaaaacct tccgcctgca aaacatgctg gtaaattcgt
    12781 gcttcaggtt tgcgcatccc cagatcttgc gacagataga tatggtcagc agcatcacga
    12841 atttccgggt attcttccgg ccagaaggtg gtatgcaggc ggttggtatt ggaaagcacc
    12901 accacgcgat gcccctgctc acgcagttta tgcatgatgg cgatcacttc cgggcgcagc
    12961 gcaacaaaca ccgcctgcca gccgtgagag aactgctcgt agcttagcgg tagagccatc
    13021 tcatgacaca gcgcctctgc gaacgcttcg tcgctaattt ccccacgctc atgctgatga
    13081 aacgcctccc ccatatgaaa actcttctta agcgatgcca gcggaatacg cgttaaatcg
    13141 ctccaggctc ccagcacacg gttaaagtcg atatcgacaa tcacattacc taaatcaaag
    13201 atatagagca tttttgcctc ctttcgcgcc acaagaaatt aactgtagcg ggaaaggatg
    13261 ggtttggcta tgtggcaagg tatgaagcca ggttaacggt cacaaaaatc tacgcgtctt
    13321 cctggggaag aatgatttca atttggctgt cttgtaagcg tgattttagc gcagcgggcg
    13381 gcagctcgtc ggtaaagagc gcagtgacct gtgccacgtt accaatttca accgccgccg
    13441 aagcatgata cttagtgtga tcggcgacca gcagaatatt tctcgcgtgc gccatcatcg
    13501 ttttcaccac gttagcttcg tttacatcaa actccatcaa cgcgccatcg ctctcaatcg
    13561 ccccaacgct tgttaccaga taatcagcac gaaaatcagc cacaaaggac gccgctgaag
    13621 ggccaatgat cccgctatta tgagagcgca acgtaccgcc gggcaccatc acttcaaagc
    13681 gcgggttgtg gtaaagaata tgcgccacac gcaggctgtt ggtgattatc cgcaaatgat
    13741 tatggttaag taacgcccgg gcaacatgct caacagtcgt accaatggtg ataaatattg
    13801 ttgaaccatc aggaatatag tctgccaccg cttcggcaat cgcttttttt tctcggtttg
    13861 cgaaacctca cgctgctcga acgccgtatt aacgacgctg gaagcccgac ccgcgccacc
    13921 gtgatggcgc gtaatcaggc cttgctcgct taatttacga atatcccggc ggaccgtctg
    13981 cgtggagaca tccagcagat ttgccagctc atcaatattc atatacccac gctcggcgat
    14041 cagcatgagg agttggtcgt gccgcgggtt accggttagt tcggtaaggc tcatgaaaat
    14101 tcctcgaaaa accatcaccc tggcatttta tacaaaaagt gacaaaaaag atcgggtttg
    14161 atcacagtca gggatcccgg ctcgtccacc gggacgtgtg cattttaacg ccgctacacc
    14221 gctggcgaag cggactgact ccgctaaatc cccacttgtt gccagcgcca ccgccaaagc
    14281 gccgtgaaaa acatcacccg cacctgtggt atctaccaca tcaactttga aggccggttg
    14341 atgctgacgc ccaccatttt ccagccagtc gcagcctgcg ctaccctggg tcacatagac
    14401 atgaccattt gtgagcgttt gtgcctgttt tagcgcactg gccatctctt tcacgcccgt
    14461 taagcgcgcc agacccggtt ctgaaaaggc cgcgtgatcg cttaatgcca ccagctcact
    14521 gatatcctgc ggcgtaatgt ccccgtccag aacggtcatc acacccgcct gacgggccag
    14581 ggtgaaggct tttttaacgc cgtcgtgcca gcgtacatct gccagcacaa catcccactg
    14641 agagaaatca atttcctcca accactctgc gtcaggcagc agatccgggc tggggtagtt
    14701 aatgattatc cgctcgcctt tggtatccac catgatggcg gattgcgaag atttcgcctg
    14761 gttataccgt ttggtgtaac gggtgttaac gccccaggat tccagctctg ctagcaggct
    14821 gttgcccgcg tcgtcatcac ctacgcgacc aataaaatcg acctgcgccc ccagccttgc
    14881 cgccgcaacc gctgccgtcg ccgctggccc gccaccaact tccgtataat ttctcgccac
    14941 gtatttaccg ctctccgtcg gtaacccttc cacgtaatag atgcgatcca tcacggttat
    15001 acctacacaa gcaacacgaa tcatggtcat tccttaaaca ttttgggatg cgttcatttt
    15061 catttcacca attgtttaaa aagtgacggc tgtcaatttt ttgacgataa attacaaatg
    15121 cgatcaaaaa cagacaagca aagcatgtca taaatgacaa aaagtgacat cactgtattc
    15181 aggagagggt tatggcagca atcgcgttta tcggtttagg acaaatgggt tcgccaatgg
    15241 cgagcaattt attgcagcaa gggcatcaac ttcgcgtctt tgatgtgaac gccgaagctg
    15301 tgcggcatct ggtagacaaa ggtgcgactc ccgccgccaa cccggtgcag gccgctaaag
    15361 atgccgaatt tatcattacc atgttgccga atggcgatct ggtgcgcaac gtgttgttcg
    15421 gtgaaaacgg cgtttgcgaa ggcttatcta ccgatgcgct ggtcattgat atgtccacca
    15481 tccatccgct gcaaaccgat aaattgattg ccgatatgca agccaaaggc ttcagcatga
    15541 tggatgttcc ggtaggccgt acttctgcaa atgccattac cggtactctg ttactgctgg
    15601 ctggcggcac cgctgaacaa gttgaacgtg ccacaccgat cctgatggcg atgggcagtg
    15661 agttgatcaa cgctggcggt ccgggcatgg ggatccgcgt taagctcatc aacaactaca
    15721 tgagcatcgc gctcaatgcg ctttcggcag aagccgccgt tttgtgcgaa gccctgaatc
    15781 ttcccttcga tgttgccgtc aaagtgatga gcggtaccgc cgccggtaaa ggtcacttca
    15841 ccacttcctg gccaaacaaa gtcctcagcg gagatctttc tcccgccttc atgatcgatc
    15901 ttgcccataa ggatcttggc atcgcccttg atgtcgccaa ccagctgcat gtgccaatgc
    15961 cgctgggggc cgcctcacgg gaggtttata gccaggcgcg cgcatcgggc ggttttgcca
    16021 tgctggcggt cgatcagcgc gaagccatgc gcatgatgtt tgccgcggct ggggcacccg
    16081 ctccggtagc cgatagcgtt ttaactgatt tcaaagttaa cgctgcaaag gccctctcgc
    16141 cttatgcctc ggcgattctg gtagatcaac aattctgcta ccgccaggtg gttgagcaaa
    16201 acgcgattgc caaaagttgc gccatgattg tcgccgccga tgagttcatt cctggcaacg
    16261 gtattccggt cgatagcgtg gttattgacc gcaaaatcaa tccgctacag atcaaacaag
    16321 acggtggcaa agccttaaaa ctgctggtgc tgtggcgtag tgatgaagat gcgcagcaac
    16381 gtctggatat ggtgaaagag ttcaacggac tgtgccactc acacggtctg gtaagcatca
    16441 ttgagccagt cgtccgccca ccgcgtcgtg gcgataaatt cgatcgcgaa caagcggtca
    16501 tcgatgccgc caaagagctg ggcgacagtg gcgctgacct ctacaaagtt gaaatgccgc
    16561 tttatggcaa aggtccgcaa caagagcttc tctgtgcttc acaacgtctg aatgaccata
    16621 tcaatatgcc atgggtgatc ctctcttcca gtgtcgacga aaaactgttc ccgcgtgccg
    16681 tacgcgtggc aatgacggcg ggcgcatcgg gattcctggc aggccgtgca gtctgggcat
    16741 cggtcgtcgg tttaccagac aacgagctga tgctccgtga cgtttgcgca ccgaaattac
    16801 aacaacttgg cgatatcgtc gacgaaatga tggctaaacg ccgctaagat gaggataaaa
    16861 ggaatgaaat ggtttaacac cctaagccac aaccgttggc tggaacagga aaccgaccgc
    16921 atctttgatt ttggtaaaaa ttccgtagtg ccgactggtt ttggctggtt aggcaataaa
    16981 gggcaaatca aagaagagat gggcacccat ctgtggatca cagctcgtat gttgcacgtt
    17041 tattccgttg ctgcggcgat gggtcgacct ggcgcttacg cgttggttga tcacggtatt
    17101 aaagccatga acggcgcact gcgtgataaa aaatatggcg gctggtatgc ctgcgtgaat
    17161 gacgagggcg tggtggatgc ctccaaacag ggctatcaac acttctttgc tctgctgggt
    17221 gccgccagcg ccgtcacaac gggtcacccg gaagcgcgca agctgctcga ttacaccatt
    17281 gagattatcg agaaatattt ctggagcgaa gaagagcaga tgtgcctgga atcctgggac
    17341 gaagccttca gcaaaaccga agagtaccgc ggcggcaatg ccaatatgca cgcggtggaa
    17401 gctttcttga ttgtttatga cgtcactcac gacaaaaaat ggctggatcg cgcgattcgc
    17461 gtggcttccg tgattatcca cgacgtcgcc agaaataatc attatcgcgt taacgaacac
    17521 ttcgataccc agtgggatcc gctgccggat tacaacaaag ataacccggc gcatcgcttc
    17581 cgcgcgttcg gaggtacacc aggccactgg atcgaatggg gccgtttaat gctgcacatc
    17641 catgctgccc tggaagcccg ttgcgaacaa ccaccagcat ggctgctaga agatgccaaa
    17701 ggtctgttta acgccaccgt gcgcgatgcc tgggcacccg atggcgcgga cgggattgtt
    17761 tataccgttg actgggaagg aaaaccggtg gtccgcgaac gtgtacgttg gcctatcgtc
    17821 gaagcaatgg gtacggccta cgcgctctac accgtcactg gcgatcgcca gtatgaaacc
    17881 tggtatcaaa catggtggga gtactgcatt aagtacctga tggactatga aaatggttcc
    17941 tggtggcagg agctggatgc ggacaataag gtcatcacca aagtctggga cggcaaacag
    18001 gatatttatc acctgctgca ttgcctggtg atcccgcgta tcccgttagc gcccggcatg
    18061 gctccagcgg ttgcagcggg tctgctggat attaatgcga aataactgat ctgaaaatag
    18121 ggtgcgggtt ttcccctcac cctaaccctc tccccaaagg ggcgagggga ccgtccggtg
    18181 cagtttttgg gtttcacatc agcctcacgg gaggctgtat gtcgttaatt aaggtgcctt
    18241 gtatgcaaat caccaatatg cattgcagtg gacagaccgt ttcacttgcc gcgggggatt
    18301 atcacgcgac cattgtgacc gtcggtgctg gtctggctga attaaccttt cagggttgtc
    18361 atttagtcat cccgcacaaa cccgaagaga tgccgctggc gcatctgggt aaagtgctta
    18421 ttccgtggcc gaaccggatc gccaacggct gttaccgtta tcaggggcag gaatatcagt
    18481 tacccattaa tgaacatagt tcaaaagccg ctattcacgg gctgcttgcc tggcgagact
    18541 ggcaaattag tgaattaacc accaccagcg ttaccctgac agcttttctg ccgcccagtt
    18601 acggctatcc gtttatgctg gcttcacaag ttgtttattc gctcaacgca cacacgggtc
    18661 tttccgttga gatcgcaagc cagaatatcg gcactgtcgc agcgccttat ggcgtgggta
    18721 ttcatcctta tttaacctgc aacttaacat ctgttgatga atatctgttc cagcttccgg
    18781 caaaccaggt ttatgccgtc gatgaacacg taaatccgac aacgcttcac cacgttgatg
    18841 agctggattt aaatttcacg caggcgaaaa aaatagccgc gacaaaaatc gaccacacct
    18901 ttaaaaccgc taatgatttg tgggaaatga ccattaccca tccacagcag gcgctttcgg
    18961 taagcctttg ttccgatcaa ccctgggtgc aggtttatag cggtgaaaaa ttacagcgtc
    19021 agggactggc agttgaacct atgagttgcc cgcctaacgc ctttaattca ggtatcgact
    19081 tattattact ggaaccagga aaaccgcacc gcctgttttt taatatttac ggacagagaa
    19141 aataacttgt attgattagc agtcaaaatc attgaagaac atttcactcg ctaaaggcaa
    19201 cattatgtat cttcaattta ttaagttaaa aacagctcag cttctgtaaa gtttttatat
    19261 atcagtttta tgagaatcat tttacataag cctgtaataa caggaaggca acaatctcgt
    19321 tctcaactct atggagatta gttatggata cgccacgtcc acagttatta gattttcaat
    19381 ttcatcagaa taacgacagt tttaccctac attttcaaca acgtcttatt ttaacccata
    19441 gcaaagataa tccttgttta tggattggct caggtatagc ggatatcgat atgttccgcg
    19501 gtaatttcag cattaaagat aaactacagg agaaaattgc gcttaccgac gccatcgtca
    19561 gccagtcacc ggatggttgg ttaattcatt tcagccgtgg ttctgacatt agcgccacgc
    19621 tgaatatctc tgccgacgat cagggccgtt tattgctgga actacaaaac gacaacctta
    19681 accacaaccg tatctggctg cgccttgccg ctcaaccaga ggaccatatc tacggctgcg
    19741 gcgaacagtt ttcctacttc gatctgcgcg gcaaaccgtt cccgctatgg accagtgaac
    19801 aaggcgttgg tcgcaacaaa caaacctatg tcacctggca ggccgactgc aaagaaaatg
    19861 cgggcggcga ctattactgg actttcttcc cacagcctac gtttgtcagc acgcagaagt
    19921 attactgcca tgttgataac agttgctaca tgaacttcga ctttagcgcc ccggaatacc
    19981 atgaactggc gctgtgggaa gacaaagcta cgctacgttt tgaatgtgct gacacataca
    20041 tctccctgct ggaaaaatta actgccctgc tgggacgcca gccagaactg cccgactgga
    20101 tttatgacgg ggtaacgctc ggcattcatg gcgggacgga agtgtgccag aagaaactgg
    20161 acaccatgcg taacgcgggc gtgaaggtta acggcatctg ggcgcaggac tggtctggga
    20221 tccgtataac ctcttttggc aagcgcgtga tgtggaactg gaagtggaac agcgaaaact
    20281 acccgcaact ggattcacgc attaagcagt ggaatcagga gggcgtgcag ttcctggcct
    20341 atatcaaccc gtatgttgcc agcgataaag atctctgcga agaagcggca caacacggct
    20401 atctagcaaa agatgcctct ggcggtgact atctggtgga gtttggcgag ttttacggcg
    20461 gcgttgtcga tctcactaat ccagaagcct acgcctggtt caaggaagtg atcaaaaaga
    20521 acatgattga actcggctgc ggcggctgga tggctgactt cggcgagtat ctgcccaccg
    20581 acacgtacct gcataacggc gtcagtgccg aaattatgca taacgcctag cctgcgctgt
    20641 gggcgaagtg taactacgaa gcccttgaag aaacaggcaa gctcggcgag atcctcttct
    20701 ttatgcgcgc cggttctacc ggtagccaga aatactccac catgatgtgg gcgggcgacc
    20761 agaacgtgga ctggagtctc gacgatggcc tggcgtccgt agtcccggcg gcgctgtcgc
    20821 tggcaatgac cggacatggt ctgcatcaca gcgacattgg cggttacacc accctgtttg
    20881 agatgaagcg cagcaaagag ctgctgctgc gctggtgcga tttcagcgcc ttcacgccga
    20941 tgatgcgcac ccacgaaggt aaccgtcctg gcgacaactg gcagtttgac ggcgatgcgg
    21001 aaactatcgc ccattttgcc cgtatgacta ccgttttcac caccctgaaa ccttatctga
    21061 aagaggccgt cgcgctgaat gcgaagtccg gtctgccggt catgcgcccg ctgttcctgc
    21121 attacgaaga cgatgcacat acctattccc tgaaatatca gtacctgtta ggccgcgaca
    21181 ttctggtcgc tccggtacac gaagaaggtc gcagcgactg gacgctctat ctgccggagg
    21241 ataactgggt acatgcctgg acgggtgaag cgttccgggg cggggaagtt accgttaatg
    21301 cgcccatcgg caagccgccg gtcttttatc gcgccgatag cgaatgggcg gcactgttcg
    21361 cgtcgttaaa cagcatctaa tcacatccgc ccgtggggaa actcatgggc aaagggagaa
    21421 catcgatgag tcacatcaca acggaagatc cagcaacttt acgcctgccc tttaaagaga
    21481 aactctctta cggtattggc gacctggcct ctaacatcct gctggatatc ggtacgcttt
    21541 atcttttgaa gttttatacc gacgttctgg ggctgccagg cacctatggc ggcattatct
    21601 ttttgatttc aaaattcttt actgcgttta ccgatatggg taccggcatt atgttggatt
    21661 cccgacgcaa gatcggtcca aaaggtaagt tccgtccttt tattctgtat gcgtcattcc
    21721 cggtcacctt actggcgatc gccaactttg tcggcacacc gtttgatgtc accggtaaaa
    21781 cggtgatggc cactattctg tttatgcttt acggactgtt tttcagcatg atgaactgct
    21841 cctacggcgc aatggtcccg gcaatcacca aaaaccccaa cgaacgcgcc tcactggcgg
    21901 catggcgtca gggcggcgct acgctgggcc tgctgctgtg cacggtggga ttcgtgccag
    21961 ttatgaatct tatcgaaggt aatcagaaac ttggctatat cttcgccgcc acgctgtttt
    22021 cactgttcgg cctgctgttt atgtggatct gctactcagg cgtgaaagag cgttatgtcg
    22081 aaacccaacc aaccaatccg gcgcaaaagc ctggcctgtt gcaatctttc cgcgcaattg
    22141 ccggtaaccg cccactgttc attctgtgta ttgccaacct ctgcacctta ggggcgttta
    22201 acgtcaagct cgccattcag gtctattaca cccagtacgt acttaacgat cccatcctgt
    22261 tgtcgtatat gggatttttc agcatgggct gtattttcat cggcgtgttc ctgatgcctg
    22321 gcgcagtcag acgttttggt aagaagaagg tctatatcgg cggcctgctg atttgggtgc
    22381 tgggcgatct gctcaactat ttcttcggcg gcggctcggt cagctttgtg gcgttctcct
    22441 gcctggcgtt cttcggctca gcgtttgtta acagcctgaa ctgggcgctg gtttccgaca
    22501 ccgtcgagta cggcgagtgg cgcaccggcg tgcgttcgga aggcacggtc tacaccggct
    22561 tcaccttctt tcgcaaagtg tctcaggcgc tggctggttt cttccccggc tggatgctga
    22621 cgcaaattgg ctatgtgccg aacgtcgcac aggctgacca caccattgaa gggttacgcc
    22681 agttgatctt catctaccca agcgcactgg cggtagtcac cattgtggcg atgggttgct
    22741 tctacagcct gaacgagaag atgtatgtcc gcattgtgga agagatagaa gcccgtaaac
    22801 gcacggcgta ataataataa acgacgccct gcggggcgtt ataaggagtg attatgtctg
    22861 accataatcc actgacatta aaactgaatc tgcgggaaaa aatcgcctat ggtatgggcg
    22921 acgtcggttc gaatttaatg ctctgcatcg gtactctgta tctcctcaaa ctttataccg
    22981 atgaattagg gatgcctgct tactatggcg ggattatctt tctggtcgcg aagtttttta
    23041 ccgcgtttac cgatatgctc accggatttt tactcgactc gcggaaaaat attgggccta
    23101 aaggcaaatt tcgtcccttt attttatatg cagcagttcc ggcggcgtta attgcaacgc
    23161 tccagtttat cgccaccacc ttttgtttgc cggttaaaac gacgattgcc accgcgctgt
    23221 ttatgatgtt tggactttca tacagtctga tgaactgctc gtatggtgcg atgatcccgg
    23281 caattaccaa aaacccgaat gagcgcgcgc agctcgcggc ttaccgccag ggtggtgcaa
    23341 ccatagggct attgatttgt accgtggcgt ttattccgtt gcagtcgctt ttttctgact
    23401 caaccgtcgg ttatgcctgt gcggcactta tgttctccat tggcggcttt atttttatga
    23461 tgctgtgcta cagaggcgtc aaagagcatt atgtggacac agcgccaacc ggacataaag
    23521 ccagtattct caaatctttt tgcgcgatat ttcgtaatcc accattgctg gttttatgca
    23581 ttgctaacct gtgtactctg gcggcattta atatcaaact ggcgattcag gtctattaca
    23641 cccagtatgt gctgaatgat attaatttat tgtcgtggat gggattcttc agtatgggat
    23701 gcatcctcgt cggcgtatta ctggtgccag taactgtaaa atgttttggt aaaaaacagg
    23761 tttatttagc tggcatggtg ctatgggcgg tgggtgatat actgaattat ttctggggaa
    23821 gtaactcttt cactttcgtc atgttctctt gtgtcgcctt ttttggcacg gcctttgtaa
    23881 acagcctgaa ctgggcactg gtaccagata ccgtagatta cggtgaatgg aaaaccggta
    23941 ttcgtgccga aggttctgtt tataccggtt ataccttctt tcgtaaaatt tctgccgcac
    24001 ttgctggctt cttgccaggc attatgctga cgcaaattgg ttatgttccc aacatcgcgc
    24061 aaagcgatgc gacattacag ggtttgcgtc agctcatttt tatctggcct tgtgcactgg
    24121 caattattgc tgcattgacg atgggattct tttacacact caatgaaaaa cggtttgcat
    24181 tgattattga ggaaatcaac caacgaaaaa ataaagagat ggcgacagaa aaaaaaacgg
    24241 cttccgtaac gttataaatg tcaatagccg ctatttccat cctggtgggt ggcggcctcc
    24301 ctacgtttaa aaaatggact tattatgaaa aagattaatg caataatttt attatcttct
    24361 ctgacctctg cctcggtatt tgctggcgca tacgtcgaaa acagggaggc atataatctt
    24421 gcctccgatc agggcgaggt tatgctacgt gtgggttata acttcgatat gggcgcgggt
    24481 attatgttaa ccaataccta cacctttcag cgagaagatg aactaaaaca tggctataat
    24541 gaaattgaag gttggtatcc tttattcaaa ccaaccgata aattaaccat ccagcccggt
    24601 ggctt
//
LOCUS       DAJVQH010000051        24445 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJVQH010000051 DAJVQH010000000
VERSION     DAJVQH010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.051"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..487)
                     /locus_tag="OQM87_002121"
     CDS             complement(<1..487)
                     /locus_tag="OQM87_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCS1508257.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LL"
     gene            760..948
                     /locus_tag="OQM87_002122"
     CDS             760..948
                     /locus_tag="OQM87_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCS1508258.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            960..1670
                     /gene="bcsQ"
                     /locus_tag="OQM87_002123"
     CDS             960..1670
                     /gene="bcsQ"
                     /locus_tag="OQM87_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCS1508259.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1667..4280
                     /gene="bcsA"
                     /locus_tag="OQM87_002124"
                     /pseudo
     CDS             1667..4280
                     /gene="bcsA"
                     /locus_tag="OQM87_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            4291..6630
                     /gene="bcsB"
                     /locus_tag="OQM87_002125"
     CDS             4291..6630
                     /gene="bcsB"
                     /locus_tag="OQM87_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCS1508260.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6637..7743
                     /gene="bcsZ"
                     /locus_tag="OQM87_002126"
     CDS             6637..7743
                     /gene="bcsZ"
                     /locus_tag="OQM87_002126"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCS1508261.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7802..7978
                     /locus_tag="OQM87_002127"
                     /pseudo
     CDS             7802..7978
                     /locus_tag="OQM87_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            8306..10054
                     /gene="bcsC"
                     /locus_tag="OQM87_002128"
                     /pseudo
     CDS             8306..10054
                     /gene="bcsC"
                     /locus_tag="OQM87_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10136..12124
                     /gene="hmsP"
                     /locus_tag="OQM87_002129"
     CDS             10136..12124
                     /gene="hmsP"
                     /locus_tag="OQM87_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCS1508262.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12308..13594
                     /gene="dctA"
                     /locus_tag="OQM87_002130"
     CDS             12308..13594
                     /gene="dctA"
                     /locus_tag="OQM87_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCS1508263.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFSSGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13815..15311
                     /locus_tag="OQM87_002131"
     CDS             13815..15311
                     /locus_tag="OQM87_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1508264.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15407..16336)
                     /gene="kdgK"
                     /locus_tag="OQM87_002132"
     CDS             complement(15407..16336)
                     /gene="kdgK"
                     /locus_tag="OQM87_002132"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCS1508265.1"
                     /translation="MSKKIAVIGECMIELSEKDADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16568..17335
                     /gene="pdeH"
                     /locus_tag="OQM87_002133"
     CDS             16568..17335
                     /gene="pdeH"
                     /locus_tag="OQM87_002133"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCS1508266.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17405..19465
                     /locus_tag="OQM87_002134"
     CDS             17405..19465
                     /locus_tag="OQM87_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1508267.1"
                     /translation="MSKAGKITAAISGVFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19647..20969)
                     /locus_tag="OQM87_002135"
     CDS             complement(19647..20969)
                     /locus_tag="OQM87_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS1508268.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21380..22393)
                     /gene="yhjD"
                     /locus_tag="OQM87_002136"
     CDS             complement(21380..22393)
                     /gene="yhjD"
                     /locus_tag="OQM87_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCS1508269.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22442..23341)
                     /locus_tag="OQM87_002137"
     CDS             complement(22442..23341)
                     /locus_tag="OQM87_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1508270.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            23861..>24445
                     /locus_tag="OQM87_002138"
     CDS             23861..>24445
                     /locus_tag="OQM87_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1508271.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMM"
BASE COUNT         5783 a         6154 c         6733 g         5775 t
ORIGIN      
        1 caagcaacaa tgaaaattgt ttatcgttat tatttccggg attaattacc aaaagcgaac
       61 aatggtttaa cctgctccat ttattcattt tatccaacca tgagcgaagc cgctcggcag
      121 gaatgttttg ccatgcgtta tttgcgcaaa caagaataaa aaggtaatta tggggatcaa
      181 tagaacactg caaatcacgg gccaaatagt atagaccttt ttcatgattt agcattgaaa
      241 ataattttat tttttccggc ccttgagaat catctaattg aaagattttc gccggatcgc
      301 tgtccatgct aatgaccgcg acgtgtgcgg tttcagcctg ggatgcaatt gtttgattcg
      361 ccagactgat agcatcttca tggcgatcga cgttaaacca ccagacgccg cctgctggca
      421 tatgtcgcag ctcatcccat aatgatgaga taccgataga gaatacaggg tccacaatgt
      481 ccctcatgtc accattttgt ctgattatca gtttactagc gaaagctaag aaataaacct
      541 accattgaaa ttaaaactta tcagatttag catgtgaatc aatttagaag gtcacaagac
      601 gtgtgatttt agtcttttcg tttttaactt tttctgttgc gtaacggaaa gtcaaaaagt
      661 gagcaaattc ccgtctcgcc gtaaacaata aaccggcgaa atgctcacgg ttagaattgt
      721 atcatgaatg gtacggttat ttcataggga tcaagcaaaa tgaataacaa tgaaccagat
      781 actctgcctg atcccgcgat aggctatatc ttccagaatg atattgtggc gttaaagcag
      841 gcattttcac tgcctgatat tgattatgcc gatatttccc aacgcgaaca gttggccgcg
      901 gcattaaaac gctggccgtt gctggcagag tttgcgcaac aaaagtaggg gattggtgaa
      961 tggccgtact gggattgcag ggggtgcggg gaggcgtggg gacaacaacc atcaccgccg
     1021 cattagcctg gtcattacaa atgttgggag aaaatgtcct agtggtcgat gcctgcccgg
     1081 acaacttgtt gcgcctgtca tttaatgttg attttaccca ccgtcagggc tgggccagag
     1141 cgatgctgga tggccaggac tggcgtgacg ctgggttgcg ctacacctcg cagctcgatt
     1201 tgctgccttt tggtcagtta tccattgaag aacaagaaaa tccacagcac tggcaaaccc
     1261 ggctgagcga tatttgctcc ggcttacagc aactaaaagc cagcgggcgt taccagtgga
     1321 ttttaatcga cttaccgcgt gatgcctcgc agataaccca ccagctgctg agtttgtgcg
     1381 agcactcgct ggcaatcgtc aatgtggatg ccaactgcca tatccgactg catcagcaag
     1441 cgctgccgga tggcgcacat attttgatta atgacttccg tattggcagt caggttcagg
     1501 acgatattta ccagctttgg ttgcaaagcc agcgccgatt actgccgatg ctcattcatc
     1561 gtgatgaagc gatggctgaa tgcctggcgg ctgaagagat actgacgctg gcgaactggt
     1621 gcctgttgaa ctactccggg ctgaaaacgc cagtcgggag tgcatcatga gtatcctgac
     1681 ccggtggttg cttatcccgc cggtcaacgc gcggcttatc gggcgttatc gcgattatcg
     1741 tcgtcacggt gcgtcggctt tcagcgcgac gctcggctgt ttctggatga tcctggcctg
     1801 gatttttatt ccgctggagc acccgcgctg gcaacgtatt cgcgcagaac ataaaaacct
     1861 gtatacgcca acgcctcgcg tccgcgtccg ctggacccgg tccgttatct cattcaaaca
     1921 tgctggttat tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt
     1981 caggtctgca aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg
     2041 ttagccataa aacacagcat ctggatgaaa aaaaagagct cggtcatttg agtgccgggg
     2101 cgcggcggtt gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg
     2161 ttactcagcc gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggggtag
     2221 cgctgatcgt acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc
     2281 tgaccgtttc ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc
     2341 cggtcagcct ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc
     2401 tggtgctcgg ctacttccag gtagtatggc cgctgaatcg tcagccggtg ccattgccga
     2461 aggatatgtc gctgtggccg tcagtggata tctttgtccc gacttacaac gaagatctca
     2521 atgtggtgaa aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga
     2581 acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg
     2641 gggtgaaata tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg
     2701 cgctgaaata tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc
     2761 gatcgttcct gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc
     2821 agactccaca ccacttcttc tcgccggacc cgtttgaacg caacctggga cgttttcgta
     2881 aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg
     2941 acgccacttt cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg
     3001 gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc
     3061 gtggctatac ctccgtgtat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc
     3121 tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtacaa atcttccgtc
     3181 tcgataaccc gctcaccggt aaaggactga agtttgctca gcggctgtgc tacgtcaacg
     3241 ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt
     3301 tcctgctgct tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc
     3361 tgccgcatat gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact
     3421 ctttctggag tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg
     3481 tggcgctgat taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg
     3541 aagaagagta cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctactcaacc
     3601 tggtgggcgt tgcggtaggc atctggcgct acttctatgg cccgccaacc gagatgctca
     3661 ccgtggtcgt cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg
     3721 cggtatcggt agaaagcaaa caggtacgcc gatcgcaccg cgtggagatg acgatgcccg
     3781 cggcaattgc ccgcgaagat ggtcacctct tctcgtgtac cgttcaggat ttctccgacg
     3841 gtggtttggg gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc
     3901 tgttgcttaa acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg
     3961 gtaatgaagt tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc
     4021 agtgtacgtt tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata
     4081 agccgctgga aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg
     4141 cggagtttgc gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct
     4201 gggttgtatc gtttattccg cgccgcccgg agcggagcga aacggcacaa ccatcggatc
     4261 aggctttggc tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca
     4321 gtggctatgg ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa
     4381 ccactgatca atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg
     4441 caagtgatgt caggcgagca gggagctgat gcccccatcg tggcgcagaa cggtccttcg
     4501 cgtgatgtga agctgacctt tgcgcaaatc gcgccgccgc cgggcagcat ggtgctacgt
     4561 ggtattaacc cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag
     4621 gcgatgctca acctcgaata cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta
     4681 aaggtttatc tcaatgatga gctgatgggc gtgctgccag tgaccaaaga acagttgggt
     4741 aaaaaaacgc tggcgcaaat gcccattaat ccactgttta ttaccgactt caaccgtgtg
     4801 cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa acccggccag caccacgctt
     4861 tggctggatg ttgggcggag cagtggactg gatctgacct atcagaccct gaatgtgaag
     4921 aatgacctgt cacacttccc ggtgccattc tttgacccgc gcgataaccg tactaacacc
     4981 ttgccgatgg tctttgcggg tgcgccggat gttgggctgc aacaagcatc tgccattgtc
     5041 gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga acttcccggt gctctataac
     5101 caattgccgg atcgcaatgc cattgtcttt gcaaccaacg acaaacggcc ggacttcctg
     5161 cgcgatcatc cggcggtaaa agccccggtg attgagatga ttaaccatcc gcagaatcct
     5221 tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa
     5281 ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa
     5341 ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc
     5401 acatttggcg aactgaaaac ctatgaagaa cagttacaat ccagcggcct tgagccagca
     5461 gcgattaacg tttcgctaaa cctgccgccg gatctctacc tgatgcgcag taccggcatt
     5521 gatatggata ttaattaccg ctacaccatg ccgccggtga aagacagttc gcggatggat
     5581 atcagcctga ataaccagtt cctgcaatcc ttcaacctga gcagcaaaca ggaggcgaac
     5641 cgtctgctgc tgcgtattcc ggtattacaa ggtttgctgg atggcaaaac agatgtctct
     5701 attccggcgc tgaaactggg cgcgaccaac cagctgcgct tcgactttga gtatatgaac
     5761 ccgatgccag gcggttcggt agataactgt attaccttcc agccggtgca gaatcatgtg
     5821 gtgattggtg acgattccac catcgacttc tcgaagtatt accacttcat tccgatgccg
     5881 gatctacgcg cctttgctaa cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa
     5941 accatcaccg tgatgccgaa agcgcctaac gaagcacaga tggaaacgtt gctgaatact
     6001 gttggtttta tcggcgcaca gacgggcttc ccggcgatta atctgacggt gaccgatgat
     6061 ggcagcacca ttcagggcaa agatgccgac atcatgatca tcggtggtat cccggacaaa
     6121 ctgaaagacg ataagcagat cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca
     6181 ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg agagcgatcg cgcggcagaa
     6241 acccagtcaa cgctgacctc ttccggtgcg atggcggcgg tgattggctt ccagtcgccg
     6301 tataacgacc agcgcagcgt gattgcgctg ttggcagata gcccacgcgg ttatgaaatg
     6361 cttaacgatg cggtgaacga tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg
     6421 atccgcgagt acggtatcaa cagcctacgt gttggcgacg tttattacgt aggtcatctg
     6481 ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg
     6541 gcggctatca gtgtgatatt gctggcatgg gtactgtggc gtctgctgcg aattattagt
     6601 cgtcgtcgtc ttaacccgga taacgagtaa ttgaagatga atgtgttgcg tagtggaatc
     6661 gtgacgatgc tgttgttggc tgcctttagt gttcaggcag cctgtacctg gcctgcctgg
     6721 gagcagttta aaaaggatta catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg
     6781 cgcaaaatca ccacctccga agggcaaagt tacggtatgt tctttgccct ggcggctaac
     6841 gaccgtgcag ctttcgataa tattctcgac tggacgcaga acaatctcgc tcagggttct
     6901 ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg
     6961 ctggacagca attcggcctc cgatggtgat gtctggatgg cctggtcgtt gctggaggcg
     7021 gggcgtttgt ggaaagagca gcgttatacc gacatcggca gcgcgttgct aaaacgtatc
     7081 gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg
     7141 ggttttgctg aggataacag ctggcgtttt aaccccagct acatgccgcc gacgctggcg
     7201 cagtatttca cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa tcaacattta
     7261 ttgctggaaa ccgccccgaa aggcttttcg ccagactggg tgcgctatga gaaagacaaa
     7321 ggctggcagc taaaagccga aaaaacattg atcagcagct acgacgctat ccgcgtttac
     7381 atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct caaccggttt
     7441 aaaccgatgg cgacattcac tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct
     7501 acggggaaag cgcagggtaa aggaccggtc ggtttttctg ccgccatgct gcctttttta
     7561 caaaaccgcg atgcgcaggc agttcagcgc cagcgcgtgg ccgataactt tcccggcagc
     7621 gatacctatt acaactatgt gctgaccctg tttggacaag gctgggatca acaccgtttc
     7681 cgcttctcga caaaaggtga gttattacct gactggggcc aggaatgcgc aaattcacac
     7741 taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa
     7801 ccatcagcag accctgcgga tggacagcgg caacactaat gccgtgcgcg ggctggcgaa
     7861 tatttaccgc cagcagtcgc cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag
     7921 ccagcggcgc agcattgatg atatcgaacg cagcctgcaa aacgaccgtc tggcacagca
     7981 ggcagaggta ctggaaaacc agggcaaatg ggcgcaggcg gcagcacttc agcggcaacg
     8041 actggcgctg gaccccggca gcgtatggat tacttaccga ctttcgcagg atctctggca
     8101 ggccggacaa cgcagccagg ccgatacgtt aatgcgcaat ctggcgcagc agaagccgaa
     8161 caacccggag caggtttacg cttacgggct gtacctctca ggtcacaacc aggacagagc
     8221 ggcgctggcg catatcaaca gcctgccgcg cgcgcagtgg aacagcaata ttcaggagct
     8281 ggttaatcga ctgcaaagcg atcaggtgct ggaaaccgct aatcgcctgc gagaaagcgg
     8341 caaagaggct gaagcggaag cgatgctgcg tcagcaacca ccttccacgc gtattgacct
     8401 cacgctggct gactgggcgt aacaacgacg tgattacacc gccgcccgca ctgcatatca
     8461 gaatgtcctg acgcgggagc caactaacgc cgatgccatt cttggtctga cggaagtgga
     8521 tattgctgcc ggtgacaaag cggcggcacg tagccagctg gcgaaactgc ctgccaccga
     8581 taacacctcg ctgaacactc agcggcgcgt ggcgctggca caggcgcagc ttggagatac
     8641 cgcagcggcg cagcagacgt ttaataagtt gatcccgcag gcaaaatctc agccaccgtc
     8701 gatggaaagc gcgatggtgc tgcgcgacgg tgcgaagttt gaagcgcagg cgggcgatcc
     8761 aaagcaggcg ctggaaacct acaaagacgc catggtcgca tccggtgtga ctacgacgcg
     8821 tccgcaggat aacgacacct ttacccgact gacccgtaac gacgagaaag atgactggct
     8881 gaaacgcggc gtgcgcagcg atgcggcgga cctctatcgc cagcaggatc ttaacgtcac
     8941 ccttgagcac gattactggg gttcgagcgg caccggtggt tactccgatc tgaaagcgca
     9001 cactaccatg ttgcaggtgg atgcgccgta ttctgacggg cggatgttct ttcgcagtga
     9061 tttcgtcaat atgaacgtcg gcagtttctc cactaatgcc gatggcaaat gggatgacaa
     9121 ctggggcacc tgtacattac aggactgtag cggcaaccgc agccagtcgg attccggtgc
     9181 cagcgtggcg gtcggctggc gaaatgacgt ttggagctgg gatatcggta ccacgccgat
     9241 gggcttcaac gtggtggatg tggtcggcgg catcagttac agcgatgata tcgggccgct
     9301 gggttacacc gttaacgccc accgtcggcc catctccagt tctttgctgg cctttggtgg
     9361 gcaaaaagac tccccgagca ataccgggaa aaaatggggt ggcgtacgtg ccgacggtgt
     9421 ggggctaagt ctgagctacg ataaaggtga agtaaacggc gtctgggcat cgcttagtgg
     9481 cgaccagtta accggtaaaa atgtcgaaga taactggcgc gtgcgctgga tgacgggcta
     9541 ttactataag gtcattaacc agaacaatcg ccgcgtcaca atcggcctga acaacatgat
     9601 ctggcattac gacaaagatc tgagtggcta ctcactcggt cagggcggtt actacagtcc
     9661 gcaggaatac ctgtcgtttg ccataccggt gatgtggcgg gagcgcacgg aaaactggtc
     9721 gtgggagctg ggggcgtctg gctcgtggtc gcattcacgc accaaaacca tgccgcgtta
     9781 tccgctgatg aatctgatcc cgaccgactg gcaggaagaa gctgcgcggc aatccaacga
     9841 tggcggcagc agtcagggct tcggctacac ggcgcgggca ttacttgaac gacgtgttac
     9901 ttccaactgg tttgttggca cggcaattga tatccagcag gcgaaagatt acgcacccag
     9961 ccatttcctg ctctacgtac gttattccgc cgccggatgg cagggtgaca tggatttacc
    10021 gccgcagccg ctgatacctt acgccgactg gtaagttttc agatagcgcc tctcttaatg
    10081 ccgctgcgat cgggtatact cgggcggcaa tctgggattt ccggggggag acaatttgcg
    10141 cgtaagtcgc tcgttaacaa tcaagcagat ggcaatggtg gcagccgttg tcctggtgtt
    10201 cgtttttatt ttttgcaccg ttttgctgtt ccatctggtc cagcagaatc gctataacac
    10261 ggctacgcaa ctggaaagca ttgctcgctc tgtccgcgaa cccttatctt cagctatttt
    10321 gaaaggcgat attcccgaag cggaagctat tcttgccagc attaaaccgg caggcgtggt
    10381 cagccgtgcc gatgtagtgc tgcctaacca gttccaggcg ctgcgtaaaa gttttattcc
    10441 agagcgtccg gtgccggtaa tggttactcg cctgtttgag ctaccggttc aaatctcgct
    10501 gggcgtctac tcgctcgaac gtccggcaaa tccgcagcca atagcctatc tggtgctaca
    10561 ggcggattcc ttccgtatgt ataagttcgt gatgagcact ctctcaacgt tagtgaccat
    10621 ttacttactt ttgtcgctta tcctgaccgt cgccatcagc tggtgcatta accgcctgat
    10681 tttgcatccg ttacgcaata ttgctcgcga acttaacgcc atcccagccc aggagcttgt
    10741 tggtcaccaa ctggcattac cgcgtctgca tcaggacgat gaaatcggta tgttggtgcg
    10801 cagttacaac ctcaaccagc aattgctgca gcgccattat gaagaacaga acgaaaatgc
    10861 gatgcgcttc ccggtgtcgg atttgccgaa caaagccttg ctgatggaga tgctggagca
    10921 ggttgtcgcg cgtaaacaaa ccaccgcgct gatgatcatc acctgtgaaa ccctgcgtga
    10981 tactgcgggc gtgctgaaag aggcgcaacg agaaattctg ctgctgacgc tggtggaaaa
    11041 actcaaatcg gtactgtcgc cacgtatgat cctcgcgcag attagcggtt atgactttgc
    11101 tgtcattgcc aacggtgtac aggaaccgtg gcacgcaatc actttgggtc agcaagtact
    11161 cactatcatg agcgagcgcc tgccgattga acgtattcaa ctccgtccgc actgtagcat
    11221 tggcgtggcg atgttctacg gcgatctcac cgccgaacag ctttacagtc gcgctatttc
    11281 tgcggcattt accgctcgcc ataaaggcaa gaatcagatt cagttctttg atccgcagca
    11341 gatggaagca gcccagaagc ggttgacgga agagagcgat atccttaatg cactggaaaa
    11401 tcatcagttt gcaatttggt tacagccaca ggtcgagatg accagcggta aactggtcag
    11461 tgcggaagtg ttactgcgta tccagcaacc ggatggcagt tgggacctgc cggatggctt
    11521 aatcgatcgc attgagtgct gtgggctgat ggttaccgtc ggtcactggg tgctggaaga
    11581 gtcctgtcga ttgcttgcag cctggcaaga gcgcggcatt atgctgccct tgtcggtaaa
    11641 cctctctgcg ctgcaactga tgcacccgaa tatggtggcg gatatgctgg aactgttaac
    11701 ccgctatcgc attcagccgg gaacactgat tctggaagtg acagaaagcc gacgtattga
    11761 cgaccctcat gctgcggtgg caatcctccg tccgctgcgc aatgccggag ttcgggtggc
    11821 gctggatgat ttcggcatgg gctacgcagg gctgcgtcag ctgcagcata tgaaatcgtt
    11881 gccaatcgac gtactgaaaa tcgacaaaat gtttgttgaa ggcttgccgg aagatagcag
    11941 catgattgct gcaattatca tgctggcgca gagcctgaac ttacaaatga ttgccgaagg
    12001 cgtggagact gaagcacaac gagactggct ggcaaaagcg ggcgttggta ttgcccaggg
    12061 cttccttttt gctcgcccac tccctattga aatcttcgaa gagagttacc tggaagaaaa
    12121 gtagctaccc caaactgatt acaaaacttt aaaaagtgct ggtttgtgcg agccagctca
    12181 aactttttaa cctttttgtt tcaattatga tccaggtaca tttctgtgat gttgtctggg
    12241 tgttatttta aggccgcagg tacccccata accttacaag acctgtggtt ttactaaagg
    12301 acaccctatg aaaacctctc tgtttaaaag cctttacttt caggtcctga cagcgatagc
    12361 cattggtatt ctccttggcc atttctatcc tgaaataggc gagcaaatga aaccgcttgg
    12421 cgacggcttc gttaagctca ttaagatgat catcgctcct gtcatctttt gtaccgtcgt
    12481 aacgggcatt gcgggcatgg aaagcatgaa ggcggtcggt cgtaccggcg cagtcgcact
    12541 gctttacttt gaaattgtca gtaccatcgc gctgattatt ggtcttatca tcgttaacgt
    12601 cgtgcagcct ggtgccggaa tgaacgtcga tccggcaacg cttgatgcga aagcggtagc
    12661 ggtttacgcc gatcaggcga aagaccaggg cattgtcgcc ttcattatgg atgtcatccc
    12721 ggcgagcgtc attggcgcat tttccagcgg taacattctg caggtgctgc tgtttgccgt
    12781 actgtttggt tttgcgctcc accgtctggg cagcaaaggc caactgattt ttaacgtgat
    12841 cgaaagtttc tcgcaggtca tcttcggcat catcaatatg atcatgcgtc tggcacctat
    12901 tggtgcgttc ggggcaatgg cgtttaccat cggtaaatac ggcgtcggca cactggtgca
    12961 actggggcag ctgattatct gtttctacat tacctgtatc ctgtttgtgg tgctggtatt
    13021 gggttcaatc gctaaagcga ctggtttcag tatcttcaaa tttatccgct acatccgtga
    13081 agaactgctg attgtactgg ggacttcatc ttccgagtcg gcgctgccgc gtatgctcga
    13141 caagatggag aaactcggct gccgtaaatc ggtggtgggg ctggtcatcc cgacaggcta
    13201 ctcgtttaac cttgatggca catcgatata cctgacaatg gcggcggtgt ttatcgccca
    13261 ggccactaac agtcagatgg atatcgtcca ccaaatcacg ctgttaatcg tgttgctgct
    13321 ttcttctaaa ggggcggcag gggtaacggg tagtggcttt atcgtgctgg cggcgacgct
    13381 ttctgcggtg ggccatttgc cggtagcggg tctggcgctg atcctcggta tcgaccgctt
    13441 tatgtcagaa gctcgtgcgc taactaacct ggtcggtaac ggcgtagcga ccattgtcgt
    13501 tgctaagtgg gtgaaagaac tggaccacaa aaaactggac gatgtgctga ataatcgtgc
    13561 gccggatggc aaaacgcacg aattatcctc ttaatctcac cacttatgcc cgcactccct
    13621 tccccgagtg cgggtgtttg cgcataattg acccctgtgc ctgaacttac cacctgggtg
    13681 agttcaggtt tatttcactt cttccgtgac attttcatgt tcttgcggtc taacacgaag
    13741 tgtttttaac gtcatttaga ctgaccgaca acgggcagtt tttttattac caggatagtt
    13801 gatcaggggt tcacatgcag ggcataaaaa ttcgactttt agcgggcggt ttgctgatga
    13861 tggccactgc tggctatgtg caggcagatg cgctccagcc tgatccagca tggcaacagg
    13921 ggacgctttc caacggttta cagtggcaag tgctgaccac cccccagcgt cccagcgatc
    13981 gtgttgaaat tcgcctgctg gttaataccg gttcgctcgc cgaaagtaca caacagagcg
    14041 gttacagtca cgccatccct cgtattgcgc taacgcaaag cggtggcctt gacgcagcac
    14101 aggcgcgttc attgtggcag caggggatcg accctaaacg cccgatgccg ccggtaattg
    14161 tctcttatga caccacgctg tttaatctga gtttgcccaa taaccgtaac gatttgctga
    14221 aagaagcgct ctcttatctg gcaaatgcca ctggcaaatt gaccatcaca ccagaaacca
    14281 tcaaccacgc gctgcaaagt caggacatgg tggcaacctg gcctgccgat actaaagagg
    14341 gctggtggcg ctatcgtctg aaagggtcaa ccttgttagg tcacgatcct gccgatccgc
    14401 tgaaacaacc cgttgaagcg gaaaaaatta aagatttcta tcagaaatgg tacaccccgg
    14461 atgcaatgac gctactggtg gtgggaaacg tggatgcgcg ctcggttgtc gaccaaatca
    14521 acaaaacgtt tggcgaactg aaaggcaaac gtgaaacgcc agctccggtg ccgacgcttt
    14581 ctccgctgcg tgcggaagcg gtgagtatta tgactgacgc ggtgcgtcag gaccggttat
    14641 ctatcatgtg ggatacgccg tggcagccga ttcgtgaatc agccgcactg ctgcgctact
    14701 ggcgtgcgga cctggcccgc gaggcgctgt tctggcatgt tcagcaagcg ttaagcgcca
    14761 gtgacagcaa agacatcggt cttggatttg actgccgtgt gctgtatctg cgtgcgcagt
    14821 gtgccatcaa catcgaatca ccaaacgaca agctgaacag caaccttaat ctggtggcgc
    14881 gtgaactggc gaaggttcgc gataaaggtc tgccggaaga agagttcaat gcgttagtgg
    14941 cgcaaaagaa actggagctg cagaaactgt ttgccgccta tgcacgggct gataccgata
    15001 ttctgatggg ccagcggatg cgttcgttgc aaaatcaggt cgtcgatatc gcgccggagc
    15061 agtatcagaa actgcggcag gatttcctta acagcctgac tgtggagatg ttaaatcagg
    15121 atctgcgtca gcagttgtcg aatgatatgg cgttaatact gctgcagccg aaaggcgagc
    15181 cggaatttaa catgaaagcg ttgcaggcgg cctgggatca aatcatggcc ccatctaccg
    15241 ccgctgcgac cacctctgtc gccacggatg acgtacatcc tgaagtgacg gatattccac
    15301 ctgcacagta atgcctgatg gtgatgagtt agcacaacca tcaggtattt atggatgagc
    15361 tggatagagg ggtaacactt tatccagcct tttgcatatg ctgcgtttac gctggcatcg
    15421 cctcacgcgg gataatcgcg ccgcgatact gaataacggt acttgcggtc aggtgcccgc
    15481 gtttcgccgc gttttccgca ctgccgcccg tcaaacgtac cgccagataa ccggcgctga
    15541 aagagtcgcc agctgcggtg gtatcgatca ctttttcttt cggcagtttc accgccggaa
    15601 catcaactaa cgcttcacca gcaatggaca ccaggcaaga atccgccccg cgtttcacca
    15661 ccacttcttt cacgcccgcg ttatgggtgc gcgcaatgac gtcttccacc ggctgttgac
    15721 cccacagcgc gtcttcgtcg tccagcgtca ggaaggcgat atccgtgcat tccagcattt
    15781 gctggtacac ctgctgtgtc tcttctttgc tggcccacag gcgcggacga tagttattgt
    15841 cgaaaatcac ttttccgcct ttggcgcggc attcgcgcag cagggaaagc agcttttcgc
    15901 ggctggtcgg gcttaagatc gccaggctaa tcccgctcag gtagagataa tcgaaattcg
    15961 ccagctcttc gcaaatcgcc gcagactgct cgctcgccag ccagaatttg gcggcggctt
    16021 cgttacgcca gtagtagaac gtacgttcgc cggtgctgtc ggtttcaatg tagtaaaggc
    16081 ccggaagacg gttttccatc cgttgggtca gggaagtatc aacgttctcg ccgtgccagg
    16141 cgtccagcat ctgctggcta aaactgtccg ttcccagcgc cgttacgtaa tgaacggtta
    16201 atgccgcagg atcgacctga cgggcgatat agacggaagt gttcagggta tcgccgccga
    16261 aaccgcgctt aacgtccgcg tctttctcgg aaagctcaat catgcattcg ccaatcacgg
    16321 caatcttttt ggacatagtc gtgaacctga tctgtaaaaa attagcgtta gtttgcgctg
    16381 tggtggtttg ctggtcaacc atattaaaac agtgttccat tatttttttg agccagaacg
    16441 tattcctgaa agattccgtt gtggagtgag gaaatttacc ccggccaggc ggtaaagttc
    16501 tgcccttacg cgccgataat ctttgtcgag tccgggcagc atcactttta aacacaggac
    16561 atctttgatg ataaggcagg ttatccagcg aataagcaac cctgaagcaa gcatcgagag
    16621 cttgcaggaa cggcgttttt ggttgcagtg tgagcgtgct tacacctggc agccgatcta
    16681 tcaaacatgc gggcggttaa tggccgtgga gctattaacg gtggtcacgc atcccttgaa
    16741 cccttcgcaa cgcctgccgc cggatcgcta ttttactgaa atcaccgtca gccatcggat
    16801 ggaggttgtg aaagagcaga ttgatttgct ggcgcaaaaa gccgacttct ttgtagagca
    16861 cggcctgctg gcatcggtca atattgatgg ccctacgctc atcgccctgc gtcagcaacc
    16921 aaaaatcctg cgccagattg agcgtcttcc ctggctgcgt ttcgaactgg tggagcatat
    16981 ccgtctgccg aaagattcaa cctttgcctc gatgtgtgaa tttggcccgc tgtggctgga
    17041 tgattttggt accgggatgg caaatttctc tgcactaagt gaagtgcgtt atgactacat
    17101 caaaatcgcg cgagaactgt ttgtgatgct gcgtcagtcg ccggaaggcc gcacactctt
    17161 ttctcagctt ttacatctaa tgaatcgcta ttgtcgcggg gtgattgtcg agggcgtaga
    17221 aacaccggaa gagtggcgtg atgttcagaa ctcgcccgca ttcgccgcac aaggctggtt
    17281 tctttcacgc ccggcaccga tggaaacgct gaatacggcg gttctggcgc tataagctgc
    17341 ctcattttcc gccgggctgg actatcttta ggactggcac aggaagaata tgaggaagcg
    17401 aacgatgagc aaggcaggca aaataaccgc tgcgatttca ggggttttct tgttgttgat
    17461 tgtcgtggcg atcattttga ttgcaacttt tgactggaat cgactcaaac cgaccatcaa
    17521 ccagaaagtc tctgcggagt tgaatcgtcc gttcgctatc cgtggcgatc tgggcgtggt
    17581 gtgggagcgg caaaaacaag aaactggctg gcgcagctgg gtgccgtggc ctcatgtaca
    17641 tgcagaagac atcattcttg gtaatccacc ggatattccc gaagtcacga tggtgcattt
    17701 gccgcgagta gaggcaacgc tggcaccgct ggcgctgctg accaaaacgg tctggctgcc
    17761 gtggatcaag ctcgaaaagc ccgacgcgcg cctgattcgc ctctctgaaa agaacaataa
    17821 ctggacgttt aatcttgcca acgatgacaa caaagacgcg aatgcaaagc cgtcggcatg
    17881 gtcgtttcgg ctggataata ttcttttcga tcaagggcgg atcgccattg atgacaaagt
    17941 aagcaaagcg gatctggaaa tttttgttga tccgttaggc aagccgctgc cattcagcga
    18001 agttactgga tcgaaaggta aagcggataa agaaaaggtg ggcgattaca tttttggcct
    18061 gaaggcgcag ggacgttata acggtgaacc gctcacgggt acgggaaaaa taggcggtat
    18121 gctggcgctg cgtggcgaag ggacgccgtt tccggtacag gctgatttcc gttcaggtaa
    18181 tacccgtgtt gcttttgatg gcgtcgtgaa tgacccaatg aagatgggcg gtgtcgattt
    18241 acggcttaaa ttttctggcg attcactggg tgatctctat gaactgacgg gcgttctgct
    18301 gcccgatacc ccgccgtttg aaacggatgg tcggctggta gcgaaaatcg acactgaaaa
    18361 atcgtcggtc tttgattatc gcggttttaa tgggcgaatt ggcgatagcg atatccacgg
    18421 ttctctggtc tacaccaccg gaaagccacg accaaaactg gaaggtgatg tcgagtcgcg
    18481 gcaattgcgg ctggcggacc tgggaccgtt gattggcgtt gattccggga aaggggcaga
    18541 aaagtcgaaa cggtctgaac agaagaaggg cgaaaaaagc gttcagcctg cgggcaaagt
    18601 gctgccttat gaccgcttcg aaaccgataa atgggacgtt atggatgccg atgttcgctt
    18661 caaagggcgg cgcattgagc atggcagtag cctgccgatt agcgatcttt ctactcatat
    18721 catcctcaaa aatgctgacc tgcgcctgca accgctgaaa tttggcatgg cgggcggcag
    18781 cattgcggcg aatattcatc tggaaggcga taaaaagccg atgcaggggc gggcagatat
    18841 tcaggctcgt cgactgaaac tgaaagaact gatgcccgat gtggaactga tgcagaagac
    18901 gctgggggaa atgaacggtg acgcggaact acgcggtagc ggtaactcgg tggcggcgct
    18961 tttaggcaac agtaacggca acctgaaact gttgatgaat gacgggctgg tgagccgcaa
    19021 cctgatggag attgttgggc tgaatgtcgg caactacatt gtcggtgcga tatttggtga
    19081 cgatgaggtg cgggtgaact gcgcggcggc gaatctgcat attgccaacg gcgtggcgcg
    19141 cccgcagatt tttgctttcg atactgagaa cgcgttgatt aatgttaccg gcacggcaag
    19201 ttttgcttcg gaacagctgg atttgactat tgatccggag agtaaaggga ttcggattat
    19261 cacactgcgt tcgccgctgt atgtgcgtgg gacgtttaaa aatccgcagg ctggggtgaa
    19321 agccggaccg ctgattgccc gtggtgctgt tgctgcggca ctggcaacgc tggtaacacc
    19381 ggcggcggcg ttactggcac tgatctcacc ttccgaaggg gaggctaatc agtgtcggac
    19441 gatattgtcg cagatgaaga agtgatgata attgccggat gtctgacatc cggcaattaa
    19501 acagatttag atttttggca aagtgtgctt tgttcatgcc ggatgcggcg tgaacgcctt
    19561 atccggccta caaatccttg tgaattcaat atattgtatg gaaattcagt cctgataagc
    19621 gtagcgcatc aggcttttca cacttattac aacgactgat gtcgcgtctc atgggtcagc
    19681 agcagggcga ttaacgtcaa gccagccatc gccgccagat ataaccccac cgcacctaac
    19741 ccgtagttag cctgcaacca agctgcgata tacggcgcaa cggaagcccc gagaatcgac
    19801 gatacgttgt aagagaacga tgctccggtg taacgcactt ctgtcggaaa cagctctggt
    19861 aacagcgcac ccatcggccc gaaggtcagc cccatcagac ttaaccccag cagcaggaag
    19921 gcaaaaacca gaatcgggtt gccagaaccg agcagcgggt taaaggcgaa cagcgcgaac
    19981 aggatgatca gcgtggtgat gatcaccatg cttttacggc gaccaaaggc atcagccagt
    20041 aatccggcga ctggcaccat cacgccaaaa ccaataactg ccatcatcag catccacaac
    20101 acttcgttac gcggcaggcc aagcccaact ggcgcggcgg cggtactgaa ggtcatagag
    20161 tagaccgtca tgatgtaaaa cagcgtatag gttgccagca taatgaacgt acccagtacg
    20221 gttacgcgaa catgtttggt cagcagcgta cccagcggga tcttcacctg ttttttcgct
    20281 ttagcgactt tctcaaacac cggcgactca tgcagcgaca cgcgaacata caggccgata
    20341 atgaccagca ccgccgagaa gataaatggc acacgccagc cccagctcat aaactgctca
    20401 tcggtcagta gccaggaaag cagcaagaaa gtgccgttgg caaagaagaa gccaatcggt
    20461 gcgcctagct gtgggaagga gccatacagt gcacgtttgc gcggtggggc gttttcagtc
    20521 gccagcagcg ccgcgccgcc ccattcaccg cctaagccca gaccctgacc aaatcgagcc
    20581 agcgccagca gcagtggggc gaaaatacca atcgtggcat agcccggcag cagaccaatc
    20641 accacggtcg aaatccccat cgttagcaac gaggcgacca gcgtcgcttt acgcccaacg
    20701 cgatcgccaa aatgaccaaa aacggcagag ccaatggggc gcgcgacgaa ggcgatggcg
    20761 aaggtggcga gcgactgtag cgttgctgcc gttggatcgc cctgcgggaa gaagatatgc
    20821 ggaaacacaa taacggccgc agtggcgtaa atgtaaaagt cgaagaactc aatggccgtg
    20881 ccaatgagag aggcgacaag gactttatta cgcgagttga tcggcgtgta ttcttgttcg
    20941 tggtcgagtg ttgtggctgt tgcttgcata atcttttctt attttttggc taacgaatag
    21001 ccatattacg cacagcaaaa gtgatatttc aatgtgtaac aaatcgcgga aatggtcaaa
    21061 aaccaggcaa aaaggggata atgtttaggc cagtgatttt tcttctatga aatgcttcac
    21121 ataattttat aaatagtgga taaaactggt ttcaggttaa ataaaagtta ccaactggat
    21181 ttatatgctg gggttatgag ttggagtgaa attgtttgct cccaaataga tggctgaaga
    21241 ggctctgggg aagagtttca agaggttatg tgcgcattat agccggatgc gatgctggcg
    21301 catcttatcc ggcctacggg gagccaggcc gaataaaatc gtatgccgga taaggcgttt
    21361 acgccgcatc cggcctattt taaggctgcg ttttccctgg cattcgcggg tcgtctttat
    21421 attcggcggt ggcaatccac gccgcgcaaa acagcgtcaa acgggcgaag aagtagaaaa
    21481 atgccatcag ccccagcacg gaaccaaatg ctgcgccaga gggggatttc atcagcgatg
    21541 gcagggtgta ggtcatcacg attttaatca cttcaaaacc aatagcagcc agaaatgttc
    21601 cgcgaatcag ggcttttttg cgcggacggt ggcgcggcaa ccgccagaag atccagaaga
    21661 aaagcagata gttggcgaag atggaaatcg ccaggccaat caatcgccac gtcggtttca
    21721 gccactcaat gctgttcagg tgcagggcgc taataatcat ttgctgcgcc gaaccggcaa
    21781 ccgaggtgat cgaaagcgtc acaatcagcg caatcaacaa accaatcagt gaaataaaat
    21841 cacgcagata ttttacccag aacttctcct gatcttgcgg cgagcgttcc cagacatcgc
    21901 gcgactgggc acgaatcgct tcacgcaggt tacccatcca gttgatgccg gaataaagtg
    21961 ccaccgccag gccgacaagc cctacagtcg tacgctgctg aacggcggtg ttgatggtgt
    22021 ttttcaacgt ggcggctagt gtcggatcgc tgatgttttg cagaattttg tcgaagatat
    22081 cctgtagcaa catcggatgg gaggccagca caaagccccc tgcggcaaac gacaccatca
    22141 aaatcgggat catcgacaaa aatgagaaat aggtgatagc cgcaccaaac tggttaccca
    22201 gccgatcgtt aaagcgttct gtcgcgcgaa taaggtgcgc aataaccggt tggcgttgga
    22261 ctttttcggc agtggtggtg acggtttcca gcgcctggct aactttgccg cctttctcgc
    22321 tattatccaa ctgcttaatc ggctcgtgct ccagatcctg ggtgggacgt ttgatctcgt
    22381 tttcctgcgt catccgctct tttgtccttc tgtcgtggtc attcttaaaa agtatagtcg
    22441 gtcagtccag gtgctctttc attacgccag ccagccactg cataaacagg tttacacgcc
    22501 gggaaagctc ccgacgctgc gggtaaacca gcgaaaggga gagcggctcg gcacggtagc
    22561 ctggcaacac ttcaataagc cgcccggcac gcagggcttc gcgcacggca atgcgcggga
    22621 tctgaataat ccccagaccg gcaagaccgg cggcgagata gttttcgcta ctgtttaccg
    22681 tcaacatgcc gccagactta aaccactgga cgccattaac gctggcaacc tcaaaaccta
    22741 acggatgtac acccaggtgc ggtgtataac gcactattgc gtgtgaagtc agatcatcgg
    22801 ggctttgcgg ataaccaaag cgcgtcagat agtgcggact ggcacagttg accatggtta
    22861 gtttgccgag gggacgggcg ataacgccat cttccggtaa cgcgccagtg cgtatcacgc
    22921 aatcaaaacc atcatgaaga atatctaccg gacggttatg gctactcagt tccagctcaa
    22981 ttcccggatg ctgatagaga aattccgaca gacgcggcag taacaggttt ttcgcgattc
    23041 ctgacgggat gtcgatgcgt aatttaccgc tgatactggt ggcatcctgt tgaaacagac
    23101 cgtccagttc gctgagatta ctcaacacat cttttgcccg ttgataatag gtcatgcctt
    23161 ctggcgtgag tttgacccgt cgcgtagtgc gctggagaag ctgggtgcca agttgatgtt
    23221 ccagtgcctg tatctggcgc gaaacacttc cctttggcaa agcaaagaaa tccgccgcgc
    23281 gggaaaaact ttccagctcc gcgactttga tgaacaactg cattgcgtga attttatcca
    23341 tttctttgac cgattgttgt tcacagcgaa acagtaacgc gcatatcgca ttgtttattg
    23401 ttttttctaa catctaatgc cgggtttcac aatgttgtca tgacgaatgt ggcgatttct
    23461 cagcaggaaa agggcggaat agctttatga gaaaggtgaa tccccttaaa cagagcgtat
    23521 ttttcagtga ttccctttaa agtgaataga ggacgtattg gctatttgtg cttatttttg
    23581 tctcattgcc atttgttatt ttggcttgta gtcgcggaat ttcattttta attgcatgcg
    23641 tactaaccac caatataagg aaatcctcaa aattgtttta aatagtgccc tcgacatgta
    23701 atttcaggcg tttagctgta actatttata tcggtaacta ttttctacgc gtcatctctg
    23761 tagaatggcc accgccaaat ggcgaattct tattttttct cgcgggtaat acccttccac
    23821 tacttaagtc tttttaacgt attcaggtgg agattcagtt atgcaaatag tcatgtttga
    23881 caggcagtca atatttattc atggaatgaa aatcagttta cagcagcgta ttccaggagt
    23941 gagtattcag ggggtcagtc aggcagacga gttatggcaa aagctggaaa gttaccctga
    24001 agccttagtt atgctcgatg gcgatcagga tggtgagttt tgctactggt tgctgcaaaa
    24061 aaccgtggtg cagtttcctg aggttaaggt gttaatcacg gcgacggatt gcaataaacg
    24121 gtggttacag gaagttatcc attttaatgt gctggccatt gtgcctcgcg attcaaccgt
    24181 cgagacattt gcgttggcgg taaatagtgc ggcgatgggg atgatgtttc tgcctggaga
    24241 ctggcggacg acgccggaaa aggacattaa agatctcaaa tcgttaagcg cccgccagcg
    24301 ggagatttta accatgttag cagcgggcga atcaaataag gagatcggca gagcgctgaa
    24361 tatcagtacc ggaacggtaa aagcgcatct ggaatccctg tatcgccgtc tggaagtgaa
    24421 aaatcgcacc caggcggcaa tgatg
//
LOCUS       DAJVQH010000052        24112 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJVQH010000052 DAJVQH010000000
VERSION     DAJVQH010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.052"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..1270
                     /gene="glpK"
                     /locus_tag="OQM87_002139"
     CDS             <1..1270
                     /gene="glpK"
                     /locus_tag="OQM87_002139"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCS1508272.1"
                     /translation="TNQRETTIVWEKETGKPIYNAIVWQCRRTAEICEHLKRDGLEDY
                     ISSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTVDTWLIWKMTQGRVHVT
                     DYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIPI
                     SGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTGE
                     VNYALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTGLSAPY
                     WDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIRLHALRVDGGAV
                     ANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQNLDELQEKAVIEREFR
                     PGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            1500..2510
                     /gene="glpX"
                     /locus_tag="OQM87_002140"
     CDS             1500..2510
                     /gene="glpX"
                     /locus_tag="OQM87_002140"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1508273.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLNRKDPEMQVH
                     IL"
     gene            2607..3353
                     /gene="fpr"
                     /locus_tag="OQM87_002141"
     CDS             2607..3353
                     /gene="fpr"
                     /locus_tag="OQM87_002141"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCS1508274.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(3358..3786)
                     /gene="uspD"
                     /locus_tag="OQM87_002142"
     CDS             complement(3358..3786)
                     /gene="uspD"
                     /locus_tag="OQM87_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCS1508275.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLENKSDNKLYKLTKNIQCPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(3813..4112)
                     /locus_tag="OQM87_002143"
     CDS             complement(3813..4112)
                     /locus_tag="OQM87_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCS1508276.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(4324..4764)
                     /locus_tag="OQM87_002144"
                     /pseudo
     CDS             complement(4324..4764)
                     /locus_tag="OQM87_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            4865..5464
                     /locus_tag="OQM87_002145"
     CDS             4865..5464
                     /locus_tag="OQM87_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCS1508277.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            5572..6339
                     /gene="tpiA"
                     /locus_tag="OQM87_002146"
     CDS             5572..6339
                     /gene="tpiA"
                     /locus_tag="OQM87_002146"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCS1508278.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(6394..7149)
                     /gene="cdh"
                     /locus_tag="OQM87_002147"
     CDS             complement(6394..7149)
                     /gene="cdh"
                     /locus_tag="OQM87_002147"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCS1508279.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKSVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(7256..8245)
                     /gene="sbp"
                     /locus_tag="OQM87_002148"
     CDS             complement(7256..8245)
                     /gene="sbp"
                     /locus_tag="OQM87_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCS1508280.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(8565..9527)
                     /gene="pfkA"
                     /locus_tag="OQM87_002149"
     CDS             complement(8565..9527)
                     /gene="pfkA"
                     /locus_tag="OQM87_002149"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCS1508281.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(9708..10610)
                     /gene="fieF"
                     /locus_tag="OQM87_002150"
     CDS             complement(9708..10610)
                     /gene="fieF"
                     /locus_tag="OQM87_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCS1508282.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(10758..11258)
                     /gene="cpxP"
                     /locus_tag="OQM87_002151"
     CDS             complement(10758..11258)
                     /gene="cpxP"
                     /locus_tag="OQM87_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCS1508283.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            11408..12106
                     /gene="cpxR"
                     /locus_tag="OQM87_002152"
     CDS             11408..12106
                     /gene="cpxR"
                     /locus_tag="OQM87_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCS1508284.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            12103..13476
                     /gene="cpxA"
                     /locus_tag="OQM87_002153"
     CDS             12103..13476
                     /gene="cpxA"
                     /locus_tag="OQM87_002153"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCS1508285.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(13582..14256)
                     /gene="yiiM"
                     /locus_tag="OQM87_002154"
     CDS             complement(13582..14256)
                     /gene="yiiM"
                     /locus_tag="OQM87_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCS1508286.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(14405..15388)
                     /gene="kdgT"
                     /locus_tag="OQM87_002155"
     CDS             complement(14405..15388)
                     /gene="kdgT"
                     /locus_tag="OQM87_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCS1508287.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(15648..16268)
                     /gene="sodA"
                     /locus_tag="OQM87_002156"
     CDS             complement(15648..16268)
                     /gene="sodA"
                     /locus_tag="OQM87_002156"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCS1508288.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            16553..17587
                     /gene="rhaT"
                     /locus_tag="OQM87_002157"
                     /pseudo
     CDS             16553..17587
                     /gene="rhaT"
                     /locus_tag="OQM87_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(17584..18450)
                     /gene="rhaR"
                     /locus_tag="OQM87_002158"
     CDS             complement(17584..18450)
                     /gene="rhaR"
                     /locus_tag="OQM87_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCS1508289.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(18506..19342)
                     /gene="rhaS"
                     /locus_tag="OQM87_002159"
     CDS             complement(18506..19342)
                     /gene="rhaS"
                     /locus_tag="OQM87_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCS1508290.1"
                     /translation="MTVLRSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            19630..21099
                     /gene="rhaB"
                     /locus_tag="OQM87_002160"
     CDS             19630..21099
                     /gene="rhaB"
                     /locus_tag="OQM87_002160"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCS1508291.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            21096..22355
                     /gene="rhaA"
                     /locus_tag="OQM87_002161"
     CDS             21096..22355
                     /gene="rhaA"
                     /locus_tag="OQM87_002161"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCS1508292.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            22597..23421
                     /gene="rhaD"
                     /locus_tag="OQM87_002162"
     CDS             22597..23421
                     /gene="rhaD"
                     /locus_tag="OQM87_002162"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCS1508293.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            23431..23745
                     /gene="rhaM"
                     /locus_tag="OQM87_002163"
     CDS             23431..23745
                     /gene="rhaM"
                     /locus_tag="OQM87_002163"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCS1508294.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            24046..>24112
                     /locus_tag="OQM87_002164"
     CDS             24046..>24112
                     /locus_tag="OQM87_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCS1508295.1"
                     /translation="MAALTASCIDLNIQGNGAYSVL"
BASE COUNT         5984 a         6296 c         6132 g         5700 t
ORIGIN      
        1 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
       61 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
      121 tttagaagat tatatcagca gcaataccgg tctggtgatt gacccgtact tttctggcac
      181 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
      241 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
      301 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
      361 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
      421 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
      481 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
      541 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
      601 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
      661 ctatgcgttg gaaggtgcgg tgtttatggc aggcacatcc attcagtggc tgcgcgatga
      721 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
      781 caatggtgtg tatgtggttc cggcatttac cgggctgagt gcgccgtact gggacccgta
      841 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
      901 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
      961 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
     1021 gcagttccag tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac
     1081 cgcattgggt gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga
     1141 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
     1201 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
     1261 cgacgagtaa tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct
     1321 tacgcgtctt atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca
     1381 cgccgcatcc ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa
     1441 taatcccacc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
     1501 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
     1561 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
     1621 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
     1681 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
     1741 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
     1801 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
     1861 agaagctgat tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
     1921 ataacctgcg caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
     1981 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
     2041 tatttgctat tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
     2101 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga
     2161 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
     2221 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
     2281 tcgaagccgg taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct
     2341 ctgccaccgg tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg
     2401 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca
     2461 tccactatct gaatcgcaaa gacccggaaa tgcaagtgca cattctctga gtgatttgat
     2521 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
     2581 caagtcaatc aaaacaggag aaaaaaatgg ctgattgggt aacaggcaaa gtcactaaag
     2641 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
     2701 ccgccgggca atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
     2761 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
     2821 atggcaaatt aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta
     2881 gcgaagcagc tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc
     2941 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
     3001 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
     3061 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
     3121 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
     3181 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
     3241 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
     3301 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
     3361 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
     3421 tgccggcatc aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg
     3481 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
     3541 tgtcttcgga cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctc
     3601 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
     3661 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
     3721 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
     3781 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
     3841 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
     3901 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
     3961 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
     4021 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
     4081 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
     4141 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
     4201 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
     4261 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
     4321 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
     4381 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
     4441 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt
     4501 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
     4561 atcatgcaag cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa
     4621 cgccgcggtc tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc
     4681 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
     4741 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
     4801 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
     4861 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
     4921 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
     4981 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag
     5041 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
     5101 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
     5161 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
     5221 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
     5281 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
     5341 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
     5401 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
     5461 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
     5521 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
     5581 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
     5641 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
     5701 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
     5761 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
     5821 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
     5881 gagtctgacg aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg
     5941 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc
     6001 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
     6061 atcgcttatg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
     6121 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
     6181 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
     6241 cagccagaca tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
     6301 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
     6361 atatccggca cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc
     6421 ctggatttcc tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa
     6481 cacaaacgaa ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
     6541 cgcctcaggc acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc
     6601 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
     6661 gctgctgatg ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga
     6721 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
     6781 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
     6841 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
     6901 acgatacgtt gacatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
     6961 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
     7021 caaacattcc tcaaggacac tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
     7081 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
     7141 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
     7201 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc
     7261 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
     7321 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
     7381 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
     7441 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
     7501 ccactttatc gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga
     7561 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
     7621 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
     7681 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
     7741 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
     7801 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
     7861 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
     7921 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
     7981 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
     8041 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
     8101 gtttccagtg ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
     8161 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
     8221 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
     8281 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
     8341 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
     8401 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
     8461 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
     8521 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
     8581 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
     8641 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
     8701 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
     8761 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
     8821 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
     8881 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
     8941 tcaacttccg gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga
     9001 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
     9061 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
     9121 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
     9181 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
     9241 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
     9301 tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg
     9361 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc
     9421 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
     9481 cctggcgcat caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa
     9541 ctttggaatg caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
     9601 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
     9661 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
     9721 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat
     9781 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
     9841 aaaggcagag agtcttccat ttccaaatgg atctgaataa agcgggtcgg ccctgactgc
     9901 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
     9961 tcttgccgtt cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    10021 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    10081 cgatgccagc cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    10141 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    10201 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    10261 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    10321 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    10381 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    10441 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    10501 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc
    10561 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    10621 gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    10681 aaattgagga taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc
    10741 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    10801 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    10861 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    10921 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    10981 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    11041 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    11101 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    11161 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    11221 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    11281 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    11341 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    11401 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    11461 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    11521 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    11581 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    11641 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    11701 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcgatcc tgcgccgttc
    11761 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    11821 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    11881 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    11941 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    12001 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    12061 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    12121 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    12181 ttacccaagc tcgattcacg ccagatgacc aagcttctgg atagcgaaca gcgtcagggg
    12241 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    12301 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    12361 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    12421 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    12481 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    12541 cgtccggcca gcagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    12601 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    12661 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    12721 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    12781 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    12841 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    12901 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    12961 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    13021 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    13081 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    13141 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    13201 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    13261 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    13321 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcaat tgttgaaacc
    13381 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    13441 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    13501 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    13561 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    13621 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    13681 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    13741 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    13801 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    13861 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    13921 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    13981 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    14041 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    14101 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    14161 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    14221 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    14281 ccccattaga ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc
    14341 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga ttaaggaagc
    14401 aggtttattt caccgtacct aaaatttcga ttttcgctgc tctggctttg actttacgtg
    14461 accagataga ggtaagaatt ggcaccagaa tcgaagtcac aatgaccgcc gtcgctacca
    14521 gcgaagttgc tgccggagcc atcggtttaa acgcaggcac catttctgca atcagcacag
    14581 gtgtcgctac cgcggcccct gcggaactgg aagcggcaat tccggcagtg ccatcgccac
    14641 cgccaatcaa tttatcggcg ataatcaacg gaataccggt cacgataatt attgccacac
    14701 ccaacaggat ccccagcaaa ccagtctggg caatcacagt caaatcaatg gtattgccca
    14761 gcgcgaaggc aaagaatgga atcagcgttt gcaccgcttt gctgaaaaat tctcgtaatt
    14821 cagggtcaag attccccagg gcaaagccca ccaggaacgg taatactgcg ccgacgaaaa
    14881 catgcggttc aaacgaggca atcccggcag tgcccagaat aatcatcgtc atgagcggcc
    14941 cggactccaa cgacatcaac acaaatgccc cagcttcttc tttcgtaccg tactgctgca
    15001 tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc gccagcgttg
    15061 aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc gaggcaattg
    15121 ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat ttacgcagta
    15181 ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac accgccagaa
    15241 tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc cccgcccccg
    15301 gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc ccccccggga
    15361 ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt tttaagggct
    15421 gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga cattcattta
    15481 atcaataaaa gtgatcaaaa ccacatcaat tgaaacgctg ttttattttt ataggatcat
    15541 tgtttttatt ttggaattgt tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt
    15601 aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta ttttttcgcc
    15661 gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt gatgtagtcc
    15721 ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag gcccagaatc
    15781 gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg gttagcagta
    15841 gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga accaaagcgg
    15901 gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa gtcacgttcg
    15961 atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt ccagaacagg
    16021 ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc
    16081 tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggctttcc
    16141 agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg gatttccatg
    16201 gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta
    16261 tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc gtaagcagtt
    16321 ggtttattat agttaattaa atgatattga aaatgattat caatgccgca cttttcgtaa
    16381 gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact taatgccgtg
    16441 attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag cacgattttt
    16501 acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga agatgagtaa
    16561 cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag cctgttttta
    16621 cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag tcggtgggat
    16681 tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg atttctgggc
    16741 gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg gcgctatgtg
    16801 ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt cgatgggaat
    16861 tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa ttatcaacgg
    16921 caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg gcgttctggt
    16981 ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc gcaagatggg
    17041 cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga tgtgcggcat
    17101 tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg aagccgctgc
    17161 cgcacttggc gtcgatccac tgtatgtcgc tctgccaagc tatgttgtca tcatgggcgg
    17221 cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga aggatttgtc
    17281 gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt tactctcggc
    17341 actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc acgcccgcat
    17401 tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg tattgtgcgg
    17461 cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc cggtaacggt
    17521 gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca tcggcatggc
    17581 gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg
    17641 ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact
    17701 gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga cctgccgcag
    17761 atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact
    17821 gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag
    17881 ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt
    17941 gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt
    18001 agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg
    18061 cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg
    18121 aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg
    18181 caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag
    18241 atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca
    18301 gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg
    18361 gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa
    18421 ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg
    18481 tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat
    18541 cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga
    18601 atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagc agatgtcggg
    18661 ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa
    18721 gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca
    18781 cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt
    18841 tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga
    18901 tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa
    18961 ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc
    19021 catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg
    19081 agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat
    19141 gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat
    19201 gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa
    19261 aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac ggaaaaaaat
    19321 ccacactacg taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa
    19381 tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt
    19441 tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag
    19501 caaattgtga gcatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc
    19561 agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag
    19621 gatcacatta tgacctttcg caattgtgtc gccgtagatc tcggcgcatc cagtgggcgc
    19681 gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt
    19741 tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga tagcctggaa
    19801 agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt
    19861 gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc
    19921 ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc acaacagcaa
    19981 ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt caatacgctt
    20041 tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct
    20101 ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg ggaatatacc
    20161 aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga gtcgctactg
    20221 gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt
    20281 gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat
    20341 accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta tctctcttct
    20401 ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg
    20461 gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt
    20521 atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga tctcccggcg
    20581 cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat
    20641 cgctttatca atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg ggaaacggcg
    20701 caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag tctggcgctg
    20761 ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt ctcgcaactg
    20821 catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc cgatgcctgc
    20881 ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat cggcatcgag
    20941 ttaattacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc
    21001 gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag
    21061 attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct
    21121 gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc
    21181 aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg
    21241 aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat ccgggcaaag
    21301 cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg attccggggc
    21361 cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc tcgcgcgacc
    21421 agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc
    21481 tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt acgctctcgc
    21541 atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt
    21601 cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg
    21661 gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg
    21721 atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat
    21781 tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg
    21841 ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg actgaagtga
    21901 tttccgacaa gatttccgcc gccatgctgt atgtgccgca gttgctgctg cacgtcagcc
    21961 gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc caggcaattg
    22021 ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt gacttcttcg
    22081 atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc
    22141 tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt
    22201 acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgtggcag gcggtctggg
    22261 aaatgtattg ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg
    22321 cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg gcacaagcgc
    22381 cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc ggccctctcc
    22441 ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg
    22501 gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt
    22561 ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg
    22621 tttgtccagg gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc
    22681 aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta tcacgacaac
    22741 ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact ggcaaatacg
    22801 ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga tcctgcagct
    22861 aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct ctgggggtta
    22921 accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca ctgcgagcgc
    22981 attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc
    23041 gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc
    23101 agcaccgaat gtctggcagt attcccggat ggcgttggca ttctgccgtg gatggtgccc
    23161 ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg
    23221 tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt tggtttaatc
    23281 gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa
    23341 cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta
    23401 gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggta
    23461 aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc ggaactggaa
    23521 gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa agcgcgtaat
    23581 ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact
    23641 gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccagataac
    23701 agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgccg
    23761 gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc
    23821 ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca
    23881 ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc
    23941 tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc
    24001 tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc agctcttact
    24061 gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct ga
//
LOCUS       DAJVQH010000053        23907 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJVQH010000053 DAJVQH010000000
VERSION     DAJVQH010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23907
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.053"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..323
                     /locus_tag="OQM87_002165"
     CDS             <1..323
                     /locus_tag="OQM87_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1508296.1"
                     /translation="PANNEKYLAYLQQAATLGSSNAQAEIGYLYLIGKELPQNLPDAG
                     VWFKKAAAQGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGGIEPALKALNELE
                     HQTK"
     gene            376..2106
                     /locus_tag="OQM87_002166"
     CDS             376..2106
                     /locus_tag="OQM87_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508297.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWMKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(2166..2657)
                     /gene="yajQ"
                     /locus_tag="OQM87_002167"
     CDS             complement(2166..2657)
                     /gene="yajQ"
                     /locus_tag="OQM87_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCS1508298.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2825..3736
                     /gene="panE"
                     /locus_tag="OQM87_002168"
     CDS             2825..3736
                     /gene="panE"
                     /locus_tag="OQM87_002168"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCS1508299.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3699..4289
                     /gene="yajL"
                     /locus_tag="OQM87_002169"
     CDS             3699..4289
                     /gene="yajL"
                     /locus_tag="OQM87_002169"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCS1508300.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(4343..5791)
                     /gene="thiI"
                     /locus_tag="OQM87_002170"
     CDS             complement(4343..5791)
                     /gene="thiI"
                     /locus_tag="OQM87_002170"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCS1508301.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5997..6239
                     /gene="xseB"
                     /locus_tag="OQM87_002171"
     CDS             5997..6239
                     /gene="xseB"
                     /locus_tag="OQM87_002171"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCS1508302.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            6239..7138
                     /gene="ispA"
                     /locus_tag="OQM87_002172"
     CDS             6239..7138
                     /gene="ispA"
                     /locus_tag="OQM87_002172"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCS1508303.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            7163..9025
                     /gene="dxs"
                     /locus_tag="OQM87_002173"
     CDS             7163..9025
                     /gene="dxs"
                     /locus_tag="OQM87_002173"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCS1508304.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            9080..10054
                     /gene="yajO"
                     /locus_tag="OQM87_002174"
     CDS             9080..10054
                     /gene="yajO"
                     /locus_tag="OQM87_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCS1508305.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEENSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(10108..10626)
                     /gene="pgpA"
                     /locus_tag="OQM87_002175"
     CDS             complement(10108..10626)
                     /gene="pgpA"
                     /locus_tag="OQM87_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCS1508306.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10604..11581)
                     /gene="thiL"
                     /locus_tag="OQM87_002176"
     CDS             complement(10604..11581)
                     /gene="thiL"
                     /locus_tag="OQM87_002176"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCS1508307.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDVLSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11659..12078)
                     /gene="nusB"
                     /locus_tag="OQM87_002177"
     CDS             complement(11659..12078)
                     /gene="nusB"
                     /locus_tag="OQM87_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCS1508308.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(12098..12568)
                     /gene="ribE"
                     /locus_tag="OQM87_002178"
     CDS             complement(12098..12568)
                     /gene="ribE"
                     /locus_tag="OQM87_002178"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCS1508309.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGSAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12657..13760)
                     /gene="ribD"
                     /locus_tag="OQM87_002179"
     CDS             complement(12657..13760)
                     /gene="ribD"
                     /locus_tag="OQM87_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCS1508310.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13764..14213)
                     /gene="nrdR"
                     /locus_tag="OQM87_002180"
     CDS             complement(13764..14213)
                     /gene="nrdR"
                     /locus_tag="OQM87_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCS1508311.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            14364..14903
                     /locus_tag="OQM87_002181"
     CDS             14364..14903
                     /locus_tag="OQM87_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1508312.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            15202..16086
                     /gene="tsx"
                     /locus_tag="OQM87_002182"
     CDS             15202..16086
                     /gene="tsx"
                     /locus_tag="OQM87_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCS1508313.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(16263..16610)
                     /locus_tag="OQM87_002183"
     CDS             complement(16263..16610)
                     /locus_tag="OQM87_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCS1508314.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16739..17710)
                     /gene="secF"
                     /locus_tag="OQM87_002184"
     CDS             complement(16739..17710)
                     /gene="secF"
                     /locus_tag="OQM87_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCS1508315.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17721..19568)
                     /gene="secD"
                     /locus_tag="OQM87_002185"
     CDS             complement(17721..19568)
                     /gene="secD"
                     /locus_tag="OQM87_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCS1508316.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19596..19928)
                     /gene="yajC"
                     /locus_tag="OQM87_002186"
     CDS             complement(19596..19928)
                     /gene="yajC"
                     /locus_tag="OQM87_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCS1508317.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19951..21078)
                     /gene="tgt"
                     /locus_tag="OQM87_002187"
     CDS             complement(19951..21078)
                     /gene="tgt"
                     /locus_tag="OQM87_002187"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCS1508318.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDMHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(21133..22203)
                     /gene="queA"
                     /locus_tag="OQM87_002188"
     CDS             complement(21133..22203)
                     /gene="queA"
                     /locus_tag="OQM87_002188"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCS1508319.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            22296..22869
                     /gene="acpH"
                     /locus_tag="OQM87_002189"
                     /pseudo
     CDS             22296..22869
                     /gene="acpH"
                     /locus_tag="OQM87_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(22874..>23907)
                     /gene="malZ"
                     /locus_tag="OQM87_002190"
     CDS             complement(22874..>23907)
                     /gene="malZ"
                     /locus_tag="OQM87_002190"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCS1508320.1"
                     /translation="HNLGTGGACHNSESPWRDWYLFSDDGTALDWLGYASLPKLDYQS
                     ESLVNEIYRGEDSIVRHWLKAPWNMDGWRLDVVHMLGEAGGARNNMQHVAGITEAAKE
                     TQSEAYIVGEHFGDARQWLQADVEDAAMNYRGFTFPLWGFLANTDISYDPQQIDAQTC
                     MAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLLGRDIARLPLAVVWLFTWPGVPCI
                     YYGDEVGLDGKNDPSCRKPFPWQVEKQDTALFALYRRMIVLRKKSQALRRGGCQVLYA
                     EDNVVVFVRVLNQQRVLVAINRGEASEVVLPASPFLNAVQWQCKEGHGQLTDGILALP
                     AISATVWMN"
BASE COUNT         6075 a         6268 c         5874 g         5690 t
ORIGIN      
        1 tacctgcaaa caatgaaaaa tacctagcat atctgcaaca agctgcaaca ctgggcagta
       61 gtaatgccca ggcggaaatt ggctatttat atctcatcgg aaaagaacta ccacagaatc
      121 ttcctgatgc tggtgtctgg ttcaaaaaag cagctgcaca aggtaatttt gccgcacatt
      181 tctatctggg aaggatgtat cagaatggtg acggcgttga gagaaatatg gaaaaagcca
      241 ggtttcatct atctaatgcg gcagaaggtg gaatagaacc tgcgcttaaa gcattaaatg
      301 aacttgaaca tcaaacaaaa taaaacgctc agggaccacg taaggttcat caaacagaaa
      361 aggatatagt ttgcgatgaa agcgaaatct actttgttat taatgatttt atctgttttt
      421 tattctcatc aggttttatc cagcgacccc actcagccgg atgtttattc tgtcgtcgag
      481 aaaaaactaa actctgcttt acctctggaa gaaaatacca ccttcagatc gcaggctgaa
      541 tggtttaatt tgcaatatga attattagta tctggctacc ctgaacgtgc ttacaattta
      601 ctgagcaaac ttgagacaga atcgaaaaac gctaaatatt atctggattt aactgcctcc
      661 agagtcgctg aagaagaagc accagagaca gccttaactt ttttatcaaa aattgggtta
      721 aataaaccgg ctactcttac atcctttgag agctatatca atagctggat gaaaagcaaa
      781 caacccgaaa aggctatgca gcttctcagc ctcgatgaga atgccctgaa ttattattta
      841 ccgactgtgt tagtcgcata tcagatcgtt cctgataagg caataagtat ttataattct
      901 gtatatggag ataatgttgt tataccctat cagcaagtca ccatgttact tacagttgcg
      961 gagggttatc aaacaaaagg cgatataaaa aacgccattc attatgccga taaggcgctg
     1021 aatatgtttg ataaagctat tgctgtagat agcagtcgtg aatcccatta tagtgatgag
     1081 tatttgaaac tcatgaatat ctacgccgcc aatggcaaca aagaaaaagc ggtggtttta
     1141 tctcaacgtc tacacaaagc cattaccgaa tctcaatcga attacttaag tactctggaa
     1201 ggaattttat tgttttatag aaataatgat atgcagcaag actatcaaaa cagcctttca
     1261 aattacatag tatttatcga tgaaattttt gccttttccc catcaacaag aagtgaatta
     1321 agcctcatta atttattaaa ttcattaaat gaggtggaat tgatgagaaa acgtctggcg
     1381 tttctgatga cagcaccaga atacgcttgt tatgatagtc agtattgtta tgaagacaaa
     1441 gtcagcgcat taaaaatcct gtatcgtcat catgacaatg agctaatgga caaatatttc
     1501 acgactcttc ttaaagagat cagtaatcaa ccattagaag actgggatat aataataagt
     1561 aatactggtg aaaaatttag tgaagtgggt ctaaccaaat atgctcaaca tctcgcatct
     1621 ttcgctgaag aattttatat aaaagagcaa aaatcacatt ctgaaagtga aatgagagga
     1681 ctattttcat cacttgccga attatatagt tttggtaatg atactgccag cgcaaaccga
     1741 gtgttccatc agcatgtccc tgccttcaat acagatcata tgattcaata ttttataaat
     1801 gccaaagaat ggaacaatgc cagggaatta ttgataaaag aggaaatgtt gggtatgcat
     1861 aatctgatat tgttggagaa tatttgtatg caaaaaaatg cagaatgcat ggcacacatt
     1921 acctttacgc ttaataaatt aacaacacaa ccagcaataa caaaaataga tagcgtgggt
     1981 aatgagcaac tttatcagat tggcaaaatc taccataagt tggaaattaa acctgagcca
     2041 gagcaacagg ttttaattca gtcgctttat gaccgggcat caggaagcgc ctctgcaact
     2101 caataaatgc atggtcaaaa gggccaccac ggtggccctg acaatcagta gctatatcca
     2161 gaaagttaat cgcggaagtt tttgaactgg aacggctgac cgagatcgcc accgcgcacc
     2221 atcgccatga cagcctgcaa atcatcacga gatttgccag tcacgcggat ctcatcgccc
     2281 tgaatttgcg cctgcacttt cagtttgctg tctttgatca tcttgacgat ttttttctga
     2341 gtggcgctct caatgccctg tttcagtttc gcttccacaa accaggtttt accgctatga
     2401 acgatatttt ccggtacatc cagtgaactg ccttcaatgc cgcgcttcag cagcttggca
     2461 cgcagaatat ccagcaactg attgacctgg aagtcggact cgctcaacac tttgatggtt
     2521 ttgctggcgt cgttcagctc aaatgaggct tcaacgttac ggaagtcaaa acgggactcc
     2581 acttcgcgac tcgcgttatc gaccgcgtta cgtgcttcct gaagatcaac ttcagagaca
     2641 atatcgaaag atggcatctt ttcctctccc ttcatttttg atgcgaagca taatacctgc
     2701 aaagttaaat aaccaacagt cctgcaccag gacgtatccc ttaattgtgc atagtcgcaa
     2761 cagcatgcac aattaaggga tagccttatt gaacacccgg agtggttgtg ggtgaggagg
     2821 aacaatgaaa attaccgtat tgggatgcgg tgccttaggg caattatggc ttacagcact
     2881 ttgtaaacag ggtcatgaag ttcagggctg gctgcgcgta ccgcaacctt attgtagtgt
     2941 gaatctggtt gagacagatg gttcgatatt taacgaatcg ctgaccgcca acgatcccga
     3001 ttttctcgcc accagcgatc tgctcctggt gacgctgaaa gcatggcagg tttccgatgc
     3061 cgtcaaaagc ctcgcatcca cactgcctgt aactacgcca atactgttaa ttcacaacgg
     3121 catgggcacc atcgaagaat tgcaaaacat tcagcagcca ttactgatgg gcaccaccac
     3181 ccatgccgcc cgccgcgacg gcaatgtcat tattcatgtg gcaaacggaa tcacgcatat
     3241 cggtccggca cggcaacagg atggcgatta cagttatctg gcggatattt tgcaaaccgt
     3301 gttgcctgac gttgcctggc ataacaatat tcgcgccgag ctgtggcgca agctggccgt
     3361 caactgcgtg attaatccac tgactgccat ctggaattgc ccgaacggtg aattacgtca
     3421 tcatccgcaa gaaattatgc aaatatgcga agaagtcgcg gcagtgatcg aacgcgaagg
     3481 gcatcatact tcagcggaag atttgcgtga ttacgtgatg caggtgattg atgccacagc
     3541 ggaaaatatc tcgtcgatgt tgcaggatat ccgcgcgctg cgccacaccg aaatcgacta
     3601 tatcaatggt tttctcttac gccgcgcccg cgcgcatggg attgccgtac cggaaaacac
     3661 ccgcctgttt gaaatggtaa aaagaaagga gagtgaatat gagcgcatcg gcactggttt
     3721 gcctcgcccc tggtagtgaa gagactgaag ccgtcaccac gatcgacctc ttggttcgcg
     3781 gcggtatcaa agtcaccact gccagcgtcg acagcgatgg caacctggca attacctgct
     3841 cgcgcggcgt gaagctgctg gcggatgcgc cgctggtcga agtggctgat ggcgaatatg
     3901 acgtgatcgt gctgcctggt ggcattaaag gcacggagtg ttttcgcgat agcactctgc
     3961 tggttgaaac cgttaaacag ttccaccgtt ccgggcgtat cgtcgcggct atttgcgccg
     4021 cgccagccac cgtgctggtg ccgcacgata tctttccgat tggcaatatg accggcttcc
     4081 cgacgctgaa agacaaaatt cccgctgaac aatggcagga caagcgcgtc gtctgggatg
     4141 cacgggtaaa attgctgacc agccaggggc cgggtacagc tatcgacttt ggtctgaaaa
     4201 ttatcgacct gctggttggg cgcgagaaag cccatgaagt ggcatcacaa ctggtgatgg
     4261 cggcagggat ttataattat tacgagtagt gtcggatgcg gcaaacgtcg catctgacca
     4321 gatgcgacgt aaaaactaca aattacgggc gatatacctt cacattgtta aagccttgct
     4381 cgcgcagata gagcgcctgc agacggctca tcaccccgcg ctcacaccac agcagccagg
     4441 ttttgttctg gtcgagatcg ccaaatttgg tgctcagttt atagaacggc agagaaacca
     4501 catcaatccc ttcgactttc agtggcttat cttcctgttc atcgacagaa cggatatcga
     4561 gaatcacgtc gttcgggccg aagccgttga cggtttccac ttccaccact tcctgctcgg
     4621 tctgctgggc gatttcgcgg atatcaacgt tattcgcttc ctcaaccact ttatcgagaa
     4681 tactgaagtc gaacttctct tcttccgctt caatcttcga tttaaccgct ttcaccgtcg
     4741 ggcttttgga gatcacgcca caatattccg gcatcgtgcg agcaaagtct tcggtgccaa
     4801 tctggcgagc caggttgatg atgtgctctt tgtcgtaaga aatcagcgga cgcaggatca
     4861 gcgtgtcaga gacgttatca atcaggcgca ggttggtcag cgtctgactg gacacctggc
     4921 cgagcgcttc gccggtgacc agcgcctgta cgccgtaacg ttcagccact ttagatgcgg
     4981 cacgcaccat catacgtttg aggataacgc ccatctgacc gtcgtcgatt ttctcgagaa
     5041 tttccccgac aaccggttcg aaattaatgg cgacaaaacg cacgcggtgg gagctgccaa
     5101 agcggttcca cagataatgc gccacctgac gcacgccaat ttcatgcgct gcgccgccga
     5161 ggttaaagaa gcagtaatgc acgcggcagc cgcgacgcat caacatataa ctggaaacac
     5221 cggagtcgaa accaccggaa atgagcgaca gcacatcttc ctgggtgcca atcgggaaac
     5281 caccaatacc ttcgtagcga cctttaatca gcaggaggcg atcgtcttcc acttccagat
     5341 gaaccgttac atccgggtta gtcagcttca cgcgcgcgga ttcaatatgc tgatttaaac
     5401 cgccgccgac gtaacgttcc acatcaatcg agctaaaatc atgtttgcca cggcgcttca
     5461 cgcgtacgca gaaggttttg ccttccagct gatcgcgata ctgaaccaac gctttctcaa
     5521 aaatatcgtg catgtcggta aacggcacgt cttcgacttc gagaatatgg tggatacccg
     5581 gaatacgggt cagggcgtcg cgaatagtca gacgctggtt ttcatctttc gcgcgaactt
     5641 cgatgttatc ccagtggcgg acgacagcga gcgtctcatc atagtgcttt aaaacgttac
     5701 gaatgttccc ggtaaggatt tttataaagc gcaagcgcac agattggctt ttgatggtga
     5761 tttccgggaa caatttaatg ataaacttca tggcggcaat ggttcgttgg caagccttaa
     5821 gcgacttgta tagggaaaaa tacagcagcc cacacctgcg gctgcatcca ggcgcggaag
     5881 tataccacta acatcgcttt gctgcgcaca tcaccttacc attgcgcgtt atttgctatt
     5941 tgccctgagt ccgttaccat gacggggcga aaaatattga gagtcagaca ttcattatgc
     6001 cgaagaaaaa tgaggcgccc gccagctttg aaaaggcgct gagcgagctg gaacagattg
     6061 taacccgtct ggaaagtggc gacctgccgc tggaagaggc gctgaacgag ttcgaacgcg
     6121 gcgtgcagct ggcacgtcag gggcaggcca aattacaaca agccgaacag cgcgtacaaa
     6181 ttctgctgtc tgacaatgaa gacgcctctc taaccccttt tacaccggac aatgagtaat
     6241 ggactttccg cagcaattcg aagcctgcgt taagcaggcc aaccaggcgc tgagccgttt
     6301 tatcgcccca ctgccctttc agaacactcc cgtggtcgaa accatgcagt atggcgcatt
     6361 attaggtggt aagcgcctgc gacctttcct ggtttatgcc accggtcata tgtttggcgt
     6421 tagcacaaac acgctggacg cacccgctgc tgccgtagag tgtatccacg cttactcatt
     6481 aattcatgat gatttaccgg cgatggatga tgacgatctg cgtcgcggtt tgccgacctg
     6541 ccatgtgaag tttggcgaag caaacgcgat tctcgctggc gacgctttac aaacgctggc
     6601 gttctcgatt ctaagcgatg ccaatatgcc ggaagtgtcg gatcgcgaca gaatttcgat
     6661 gatttctgaa ctggcgagcg ccagcggtat tgccggaatg tgcggtggtc aggcattaga
     6721 tttagacgcg gaaggcaaac acgtacctct ggacgcgctt gagcgtattc atcgtcataa
     6781 aaccggcgca ttgattcgcg ccgccgttcg ccttggtgca ttaagcgccg gagataaagg
     6841 gcgtcgtgct ctgccagtac tcgacaagta cgcagagagc atcggccttg ccttccaggt
     6901 tcaggatgac atcctggatg tggtaggaga tactgcaacg ttgggaaaac gccagggtgc
     6961 cgaccagcaa cttggtaaaa gtacctaccc tgcacttctg ggtcttgagc aagcccggaa
     7021 gaaagcccgg gatctgatcg acgatgcccg tcagtcgctg aaacaactgg ctgaacagtc
     7081 actcgatacc tcggcactgg aagcgctagc ggactacatc atccagcgta ataaataaac
     7141 aataagtatt aataggcccc tgatgagttt tgatattgcc aaatacccga ccctggcact
     7201 ggtcgactcc acccaggagt tacgactgtt gccgaaagag agtttaccga aactctgcga
     7261 cgaactgcgc cgctatttac tcgacagcgt gagccgttcc agcgggcact tcgcctccgg
     7321 gctgggcacg gtcgaactga ccgtggcgct gcactatgtc tacaacaccc catttgacca
     7381 attgatttgg gatgtgggac atcaggctta tccgcataaa attttgaccg gacgccgcga
     7441 caaaatcggc accatccgtc agaaaggcgg tctgcacccg ttcccgtggc gcggcgaaag
     7501 cgaatatgac gtattaagcg tcgggcattc atcaacctcc atcagtgccg gaattggtat
     7561 tgcggttgct gccgagaaag aaggcaaaaa tcgccgcacc gtctgtgtga ttggcgacgg
     7621 cgcgattacc gctggcatgg cgtttgaagc gatgaatcac gcgggcgata ttcgtcctga
     7681 tatgctggtg gtcctcaacg acaatgaaat gtcgatttcc gaaaatgttg gcgcgcttaa
     7741 caaccatctg gctcagctgc tttccggtaa gctttactct tcactgcgcg aaggcgggaa
     7801 aaaagttttc tctggcgtgc cgcccattaa agagctgctc aaacgtaccg aagaacatat
     7861 taaaggcatg gtggtgcctg gcacgttgtt tgaagagctg ggatttaact acatcggccc
     7921 ggtggacggt cacgatgtgc tggggcttat caccacgcta aagaacatgc gcgacctgaa
     7981 aggcccgcag ttcctgcata tcatgaccaa aaaaggtcgt ggttatgaac cggcagaaaa
     8041 agacccgatc actttccacg ccgtgcctaa atttgatccc tccagcggtt gtttgccgaa
     8101 aagtagcggc ggtttgccga gctattcaaa aatctttggc gactggttgt gcgaaacggc
     8161 agcgaaagac aacaagctga tggcgattac tccggcgatg cgtgaaggtt ccggcatggt
     8221 cgagttttca cgtaaattcc cggatcgtta cttcgacgtg gctattgccg agcaacacgc
     8281 agtgaccttt gccgccggtc tggcgattgg tggatacaaa cccattgtcg cgatctactc
     8341 caccttcctg caacgcgcct atgatcaggt gctgcatgac gtggcaattc aaaagctacc
     8401 ggtcctgttc gccattgacc gcgcgggcat tgttggtgct gacggtcaaa ctcaccaggg
     8461 cgcttttgat ctctcttacc tgcgctgcat accagaaatg gtcattatga ccccgagcga
     8521 tgaaaacgaa tgtcgccaga tgctctatac cggctatcac tataacgatg gcccgtccgc
     8581 ggtgcgctac ccgcgcggca acgcggttgg cgtggaactg acgccgctgg aaaaactacc
     8641 aattggcaaa ggcattgtga agcgtcgtgg cgagaaactg gtgatcctta actttggtac
     8701 gctgatgcca gaagcggcca aagtcgccga atcgctgaac gctacgctgg tcgatatgcg
     8761 ttttgtgaaa ccacttgatg aagcgttaat tctggaaatg gccgccagcc atgaagcgct
     8821 ggtcaccgta gaagaaaacg ccattatggg cggcgcaggt agcggagtga acgaagtgct
     8881 gatggcccat cgtaaaccag tacccgtgct gaacattggc ctgccggact tctttattcc
     8941 gcaaggaact caggaagaaa tgcgcgccga actcggcctc gatgccgccg gtatggaagc
     9001 caaaatcaag gcctggctgg cataatccct actccactcc tgctatgctt aagaaattat
     9061 tcatggcagg agtggcagca tgcaatacaa ccccttagga aaaaccgacc ttcgcgtttc
     9121 ccgactttgc ctcggctgta tgacctttgg cgagccagat cgcggtaatc acgcatggac
     9181 actgccggaa gagaacagcc gtcccataat taaacgcgcg ctggaaggtg gcataaattt
     9241 ctttgatacc gccaatagct attccgatgg tagcagcgaa gagatcgttg gtcgcgcact
     9301 gcgggatttc gcccgtcgtg aagacgtagt cgttgccacc aaagtgttcc atcgcgttgg
     9361 tgatttaccg gaaggattat cccgtgcaca aattttgcgc tctatcaacg acagcctgcg
     9421 acgtctcggc atggattatg tcgatatcct gcaaattcat cgcggggatt acaacacgcc
     9481 gatcgaagag acgctggaag ccctgaacga cgtggtaaaa gccgggaaag cgcgttatat
     9541 cggcgcgtca tccatgcacg cttcgcagtt tgctcaggcg ctggaactac aaaaacagca
     9601 ctgctgggcg cagtttgtca gtatgcagga tcactataat ctgatttatc gcgaagaaga
     9661 gcgcgagatg ctgcctctgt gttatcagga gggcgtggcg gtgattccgt ggagcccgat
     9721 ggcgcggggg cgactgacgc gtccatgggg agaaactacc gcacgactgg tgtctgatga
     9781 ggtggggaaa aatctctata aagaaagcga tgaaaatgac gcgcagatcg cagagcggtt
     9841 aacgggcgtc agtgaagaac tcggtgcaac acgagcacaa gttgcgctgg cctggttgtt
     9901 aagtaaaccg ggcattgccg caccgattat cggaacttcg cgggaagaac agcttgatga
     9961 gctgctgaac gcggtggata tcactttgaa gccagaacag attgccgagc tggaaacgcc
    10021 gtataaaccg catgcggtcg taggatttaa ataagagtcc aggcctgatg agacgtgaca
    10081 agcgtcacat caggcatcgg tgcacaacta cgacagaata cccagcggcc agtgatgacc
    10141 gataaagtac aggatgcctg cggaaatcac cccggcgaca atatcgtcga tcatgatccc
    10201 catgccgcca tgtacattgc gatcaaacca gcggatcggc cacggcttcc acatatccag
    10261 aatacggaaa atcacaaacc cggcggcaac ccactgccag tcattggtcg gcagcgccat
    10321 gagcgtgatc cacataccaa taaattcgtc ccagacaatg ctgccgtgat cgtgcacgcc
    10381 catatctttc gccgtttgat gacaaagata gacgccgata cagatcccca acatcaccac
    10441 cagcgagtag agctgccagg gtaaaaaggt catcagatac cagaaaggga tcgccgccag
    10501 cgatcccatc gtgccaggaa cgattgggct taatccactt ccgaatccga cagcaagcag
    10561 atgccacgga ttactcatct tcaggcgact tttcgcgaca tctttatggc gtggcaaaat
    10621 ggtcatatcc tttccagtca aacgtgacag gttcgccgtc acgaataaaa caaagccctt
    10681 cgatatcggc ggtcatttgc ccgatacagg taaacggtac gccaaggtgt ccgagagcaa
    10741 catccagcgc gccacggttc agttccggca ccgtgaaaca caactcgtaa tcttcaccgc
    10801 cagagagcgc ccagcgcagc gcctgttccg gttcaacatg gcgagaaagc acatcagaaa
    10861 acggcagcaa tgccaggtca atacgtgcgc cgcagtcgct ggctttcacg atatgcccga
    10921 gatcggaaat cagaccgtca gagagatcga tagctgaatt tgccagatcg cgcagtgcct
    10981 gcccctgtaa aatacgcggc gatggacgga gatgacgttt gatcaagtag tcagcatctt
    11041 tagcatcggc aacctgcaaa cggttttgca aaatcgccag cccggcggcg ctatcgcccg
    11101 gtgtaccggt cacatagatc cagtcacccg gtttcgcccc agagcgcgtt aaggctcgtc
    11161 ccatcggaac aaagccgtgg atacccaacg tcattgataa tggcccacgc gtggtatcgc
    11221 cgccaatgag ttgcatatcg taataattga gaagatcaaa caaactgtcg ctgaaggact
    11281 caagccacgc ttcgtctacg tccggtaagg ttaatgccag cgtcagccag gccggatcgg
    11341 cccccatcgc tgccagatcg cttaggttca ccgccagtgc tttataagcc agatcagcag
    11401 gatcgatatc agggaggaaa tggttgcccg ccaccagcgt atcagtgctg atcgccaggg
    11461 tctgtttctc ggggatattg agaagagcgc aatcgtcgcc gatgcccagt tcgacatcaa
    11521 gacgagaact tcttacacgg tcaaaataac gggcaatcag ggagaactcg ccacatgcca
    11581 tacgttatgc ctcagcaggt aaaaaagaaa aggccggcga tcatggaaca gtcttccgtg
    11641 aatctaccgg cctggatatt actttttgtt agggcgaatc acaggtgctg ctttatcgag
    11701 tacgccgttg acgaacttat ggctgtcttc tgcgccgaac gatttcgcca gttcgatcgc
    11761 ttcgttaatg gccactttgt atggcacatc actacgttta gacagttcgt acagcgcaat
    11821 gcgcagtact gctttttcta cctggcccag ttcttccagc aggcgggaca ggtagggctt
    11881 catcagtccg tcgaggtatg cggtgttagt cgccaccccg gccagcagct cacggaagta
    11941 cagaacgtca acgtctttta catcctgttc agccaggaac tggtattcaa catcagcgat
    12001 gtcgttctgg gacaactgcc aggagtagag cgcctggacg gcacactcac gagcgcggcg
    12061 acgagcagca ggtttcacgg atttcccctt actaatttca ggccttgatg gctttcaata
    12121 cattaatcat ttcaagcgcg gtcagtgcag cttctgcacc tttgttgcca gctttggtgc
    12181 cagcacgttc gatcgcttgt tcaatgcttt cagtggtcag aaccccaaaa gcaaccggaa
    12241 tttcgctgtc ctgggcaaca tgcgccaggc cgttgcttgc accaccagcg acatattcaa
    12301 agtgggcaga gccaccacga ataaccgtac ccagcgcaat caccgcgtcg tatttaccgg
    12361 ttttagccag tgcacccgcc gccagcggca gctcataggc accaggcacc caaacaacgg
    12421 taatgttttc atcttttacc tgaccgatac gtttcagtgc gtcaattgca ccttccagca
    12481 ggctgtcatt gataaagttg ttgaaacgcg cgatggtgat ggcgacgcga gcgtccgggg
    12541 tagcaacgtt agcttcaata atgttcattt tcttccttcg ggttcgagta tggccccgca
    12601 ggggggcgga ttttagcata atatttcgtg cgctgcttcc ctttcgagcc gggagatcat
    12661 gcccccacta aatgcaggca aacatccggg cctacatgac gtatctcttt gaatttaaat
    12721 tggggggcgt cggctaattt ctcaagccct ggcagcgagc ataatccacg ggcgtcgctg
    12781 cctaatagtt taggtgcgat atagacaatc agttcatcga ctaaacccgc ctgtagcaat
    12841 acgccagcga gcgttggccc cgcttccacc cagatgctgt taatctgctg tttacccagt
    12901 tgcatcatca gtacaaccag atccagatga cctttatgct ctggaatcag caaggtacgc
    12961 accgtttccg gccactcacg agaatcttcc tgcgtacgcg cgaaccaggt ttcgccgggc
    13021 tgctgcacaa tgcgatgttc cggcgtcacg cgattttggc tatcaatcac aatacgtact
    13081 ggctggcgga gattttgttg cgggtagagc gcctgagttt gttcatccag ttcagaccaa
    13141 cgcactgtta aggctggatc atccgccagc accgtggcgc tgctggttaa aatggcatga
    13201 ctttgtgcgc gctgtcgttg tacgtcgcgc cgcgcctgag gcgaagtgat ccactggctt
    13261 tcgccgctcg ccatcgccgt gcggccatca agcgatgcgc caagttttaa ctgaatataa
    13321 ggaaagccgg tgcgcatccg cttgagaaag cctttattca attgctcggc ttcactcatc
    13381 atcagcccgt ggctgacgtc aatgccagcc tgttgcagac ggtaaagtcc acgcccagcg
    13441 acctgcgggt taggatcttg catcgctgcg accacgcgcg ctacgccagc ggcgatgagt
    13501 gcgtcacagc acggtggcgt acggccatga tggctacagg gttcgagtgt cacatacgcc
    13561 gttgcgcctt tggctttttc acccgccata cgcaacgcgt gtacttcggc atgtggttca
    13621 cccgctcgtt ggtggtaacc ttcaccgaca atttcgccat ctttgacaat gacgcacccg
    13681 acattcgggt tggggtgggt ggtaaaacgt cctcgttgcg ccagctttag cgcccgcgcc
    13741 atgtaatact cgtcctgcac ggcttagtcc tccaggcgcg cgatctcttc gccaaattct
    13801 ttgatatctt cgaaactgcg gtagacagag gcaaaacgga tataggcgac tttatcgagc
    13861 tttttcaatt gctccatcac cagattgcca atcatcttgc tcggtacttc gcgctcaccg
    13921 gtggcgcgca gctgcgattt aatatgattg attgccattt caacgtaatc ggaactcacc
    13981 ggacgttttt ccagcgcccg cagcattccg ctacgcaatt tctcttcatt aaacggttca
    14041 cgcacgtcgt tgctttttac aacacgcggc ataaccagct ccgccacttc aaaggtggtg
    14101 aaacgttcat tacacaccag acactgccga cggcggcgta cggatgaacc ctcgcccacg
    14161 agacgagagt caattacctt agtgtccacg gcgaaacaga atgggcaatg catacggcgt
    14221 cctgaccagg tggttaacgg aaatctattt taccctgaac tgacgataca acaaaggcgc
    14281 aacgcttttg agataaagga tcgatgcttt cgctgctttt accctctacc attaggctaa
    14341 ttgcgacgag aaagggacga cctatgaata ctaacgtttt tcgactgctc ctgctgggaa
    14401 gcctgttcag ccttagcgcc tgtgtgcagc aaagtgaagt gcgacagatg aaacacagcg
    14461 tcagcacgct gaaccaggag atgacgcaac tcaatcaaga aacagtcaaa atcactcagc
    14521 aaaacaggct gaatgcaaaa tccagcagtg gggtttacct tctgcctgga gcgaaaacac
    14581 cggcaagact ggaaagccag atcggtactt tacgtatgtc gctggtgaat attacgcctg
    14641 atacagatgg caccaccctt acactacgta ttcagggtga gtcgaatgat cccttgcccg
    14701 cattcagcgg aactgttgaa tatgggcaaa ttcagggaac aatagacaac tttcaggaaa
    14761 tcaatgtgca gaatcaattg attaatgccc ctgccagcgt cctcgccccc agcgatgttg
    14821 atattccgtt acaattaaag gggatttctg tggatcagtt aggctttgta cgtatccacg
    14881 acattcaacc tgttatgcag taaacgtatt ttgggacgat tttgtgcgtc ccgcaacatc
    14941 tttccccgtc attttgttac tctgcttaca tcacttggat tgatagtaaa agtttgcaac
    15001 aagggcgaaa gtcagtacaa tccccgcccg aatgtgtgta aacgtgaacg caatcgatta
    15061 cgtaaatgat agaactgtga aacgaaacat atttttgtga gcaatgattt ttataatagg
    15121 ctcctctgta tacgaaatat ttagaaacgc aatttgcgcc tttttcactc ccgcaaggga
    15181 ttttcaaaca gtggcataca tatgaaaaaa acattactgg cagccggtgc ggtactggcg
    15241 ctctcttcgt cttttactgt caacgcagct gaaaacgaca aaccgcagta tctttccgac
    15301 tggtggcacc agagcgttaa cgttgtcgga agctatcaca cccgtttcgg accgcagatc
    15361 cgcaacgata cctaccttga gtacgaagca ttcgctaaaa aagactggtt cgacttctat
    15421 ggttatgcgg atgcgccggt attcttcggc ggtaactccg atgctaaagg tatctggaac
    15481 cacggttctc cgctgtttat ggaaatcgaa ccacgtttct ccatcgacaa gctgaccaat
    15541 actgacctta gcttcggtcc gttcaaagag tggtacttcg cgaacaacta catttacgac
    15601 atgggtcgta ataaagatgg tcgccagagc acctggtaca tgggtctggg taccgatatc
    15661 gacactggcc tgccgatgag cctgtccatg aacgtctatg cgaaatacca gtggcagaac
    15721 tatggcgcag cgaacgaaaa cgagtgggac ggttaccgtt tcaaaattaa atactttgtg
    15781 ccgattaccg atctgtgggg cggtcagctg agctacatcg gcttcaccaa cttcgactgg
    15841 ggttccgatt taggggatga cagcggtaac gcaatcaacg gtattaagac ccgtactaat
    15901 aactctatcg cttccagcca tattctggct ctgaactacg atcactggca ctactctgtc
    15961 gtagctcgtt actggcacga cggtggtcag tggaacgacg atgcagaact gaacttcggc
    16021 aacggcaact tcaacgttcg ctctaccggc tggggtggtt acctggtagt aggttacaac
    16081 ttctgattat gaaaatgccg ggatttattc ccggcatttc tgattgttaa caacatgtgc
    16141 tttgttcatg ccggatgcgg cgtaaacgct ttgtccggcc cacaaaagca cgtaaattca
    16201 atatattgct gaaataacgt aggcctgata agcgtagcgc atcaggcaat tttacgtttt
    16261 aatcacttct tcttgttcat catcgctttc agatcagcga atgggttgta cttcgcttca
    16321 ccgacatctt tctgcgcatc ttcccctgcg ataacggtcg taccatactg atccgcttcg
    16381 gtatatttcg aatgctcatg atcgtggcaa tagagacaca acaattccca gttactgcca
    16441 tcttccgggt tattggtatg gtcgtgatca atgtggtgaa cggtaagttc acgcaggtta
    16501 gaataaacaa actcgcggga acagcgaccg cagacccacg gatagatttt taatgctttt
    16561 tcgcgatagc cgctttctaa ccgtgcatag ttttttggga tgatagccat gaaaagttac
    16621 ctggatgaaa tgtagggtta cgcgcagttt cccaagatgc ggggattttc gcaatctcca
    16681 tacatcaaaa aaaatcccga tcttctgacc gggattttca acatcaacgg gaacttgatt
    16741 aaggcagaat tgacggctga tccgcccctt ctttttccac tttctgctgc aacatgtgtt
    16801 cgcgcttcat acccagtttc agagccaacg cagatgctac gtagatggaa gatgcagtgc
    16861 cgatggaaac accgataagc atggtcagcg agaagccttc cagtaccgga ccaccgaaga
    16921 gatacagcat caggataacc atcaaggtag taccggatgt gatcaaggta cggtgcagcg
    16981 tctgggtcaa ggacacgtta aagatttcgt aaggcgtacc gcgacggatc ttgcggaagt
    17041 tttcacgaat tcggtccgat accacgatac tgtcgttaag cgagtaaccg ataaccgaca
    17101 tcaacgatgc cacaatggtc aggtcaatct cgatatggaa taacgacaaa atacccagcg
    17161 taatgataac gtcgtgcgcc agcgcaataa ccacccctgc cgccagtcgc cactcaaagc
    17221 ggaaacctac gtacacgagg atagacagca gcgctgccat caacgccatc gcaccggttt
    17281 gcgcaaggtc tgcccccacg ctcggaccga cgaactcaat acgcttcact gctgcattct
    17341 gattggtgga ttcgttaatc accttcagaa cttggctgcc cagcacctga ccgccggttt
    17401 cgccttcagc aggcggcata cggaccatga tgtcgtggct gctaccaaag ttttgcagca
    17461 ttggctcttc aaaaccggct ttttgcaatg catcacgcat tacgtcaatt tcagccggtt
    17521 tttcgagcgt aatttcgata accgtaccac cggtgaaatc cagcccccag ttaaagccgc
    17581 gcacgcccat aataacgata gcagcgatta acagcagacc agagatgccg aaagcccagt
    17641 agtcccagcg cataaagtca tagactttac ggccgtggtt tagttgttca acagtatatt
    17701 cctgtgccac atcgcactcc tcagattgac agcttcttga cgcgcttgcc gccatatagc
    17761 aggtttacga tggcacgcgt accgacaatc gcggtaaaca tcgacgtcgc cacaccgata
    17821 ccggtagtaa tcgcgaaccc tttaattgcc ccggtaccca ctgcgtacag gatgatgact
    17881 ttaatcagcg tggtgatgtt cgcatcgaag atagaactga atgcgccacg ataaccttca
    17941 tcaattgcct gttgaacagt acgtccgttg ctcaactctt ctttaatacg ttcgttgatc
    18001 agtacgttcg catcgaccgc taccgcaagg gttaagacga tacccgcgat acctggcata
    18061 ctcagtgttg cgcctggcaa cagcgacata ataccgacga tcaagatcaa gttggcaatc
    18121 agagcactgg tcgcaatcag accaaacttc ttatagaaga tgatcatgaa cagaatagaa
    18181 accagcagac cggcaaggca agcctccagc ccctgctcaa tgttctgcat acccagggtc
    18241 gggccaattg tacgttcttc aacaatctga atcggcgcga tcaacgcacc ggcacgcagc
    18301 agcagtgaca gctgacgggc ttcgttcggg ttgttgatgc cggtgatacg gaagctgtta
    18361 cccagacgag actggatgtt ggcgatgtta atcacctctt cctgtttcac cagaaccgca
    18421 cgaccatttg catctttctt accgctgtct ttgtactcca caaacagggt tgccatcggt
    18481 ttgccgatgt tgtccttggt gaagttagac ataatgttgc caccagcgct atcgagcgag
    18541 atgttaacct gcggctggtt gtattcgtcc tggctggaag tggagtcggt gatatggtca
    18601 ccagtcagaa ttacgcgttt gtacagcaca accggctgac cttcgcgggt ctgtttcact
    18661 tcagagtcgc ccggtacgcg accggatgcc gcagcggcct gatcaacatt ggtgtttacc
    18721 agacggaatt ccagcgttgc cgtcgcgccc agaatctctt tcgcacgcgc agtgtcctga
    18781 ataccaggca gttcaacaac gatacggtca gcaccctgac gctgaaccac cggctccgcc
    18841 acgccaagtt ggtttacacg gttacgcagg atattaatgt tctgctgcac cgcatattca
    18901 cgcgcttcac tcagacgagc atcgctcatt accgcacgca gctggttgct gccctggctg
    18961 ctaatcacca ggtccggatg gcgcttgctc agatacgcaa tggcttcatc acgagcttta
    19021 gcatcgcgga aagtgatgct caggccgtag ttgttttctt tacgaacagt ggtatacggg
    19081 atgccctttt cgcgcaggtc actgcgtagg ctatcgatat tttgttcctg gagtttgcca
    19141 agcgcggtgt ccatatccac ttccatcagg aagtgaacgc cgccacgcag gtcaaggccg
    19201 agcttcatcg gctcagcatg aatagctgcc agccagcgcg gcgttgccgg ggcaaggtta
    19261 agcgccacga cgtatttgtc acccataacg cccattaatg cttcacgagc gcgcaactgg
    19321 gtgtcagtgg agtcgaagcg cgcaagaata gcgccctctt ccagtgccac agacttagca
    19381 gttatttttt cttcttgtaa cgttttctgg acctggatca gcgtttgctc actggcggcg
    19441 actccgcgcg caccagtgat ctgaacagcc ggatcctcac caaacaggtt gggaagcgca
    19501 tacagcagac cgatgacaat caccacgatc agcatgacgt acttccacaa aggataacgg
    19561 tttaacacgg caattccctt agggaaaaat tttaattaca gcgccttcat ggtgcctttc
    19621 ggcaggacgg cagctacgaa gtcacgttta ataactactt cagtggtgtc attcagcgcg
    19681 atagcaatgt agccgttttc cgctactttg gttacgcgac caaccaggcc accgttcgtc
    19741 agaacttcat cacctttggc aatggagtcc atcagctttt tgtgttcttt ggtgcgcttc
    19801 tgctgtggac gcaggatcat gaaatagaaa atcagaccga ataccaccag catcaaaatc
    19861 aaagacatcg ggctaccttg cgccggtgca cccgttgccg ctaccgcatc agaaataaaa
    19921 aagctcatta aatttccctc attattaata ttaatcaacg ttcaaaggtg gtacttctcg
    19981 cccctgacgc tggtaaaaat cagttacgaa gctctctaat ttaccctctt caatagcctt
    20041 gcgtaaaccc gccatcaaac gctggtagta acgaaggtta tgaatggtgt tgagtcgcgc
    20101 gcctaatatt tcgttgcaac ggtcgagatg atgcaagtaa gcgcgtgaat aattgcgaca
    20161 ggtgtagcaa tcacactcag gatcgagtgg gccagtatcg ctcttatatt tcgcattgcg
    20221 gattttcacc acgccatcgg tcacgaacaa atgaccattt cgggcgttgc gggttggcat
    20281 tacgcagtca aacatatcga taccgcgacg tacgccttca accaggtctt ccggtttacc
    20341 aacgcccatc aggtaacgcg gtttgtctgc cggaatttgc ggacacacat gctccagaat
    20401 acggtgcata tctgctttcg gctcacccac agccagaccg ccgacagcgt agccatcaaa
    20461 gccgatatct accagacctt taacagatat atcacgtaaa tcttcgtaaa tactgccctg
    20521 aattatacca aataacgcat ttttgttacc aagactgtca aaacgctcac ggctacgctt
    20581 cgcccaacgc agagacatct ccatagagcg ttttgcgtaa tcccagtcag caggatacgg
    20641 cgtacactca tcaaagatca tgacgatatc cgaaccaaga tcgtactgaa tctccatcga
    20701 cttttccggg tcgaggaaaa tcgggtcgcc gttgatcggg ttacggaagt gaacgccctg
    20761 ttcggtgatt ttacgaatat caccaaggct gaagacctgg aagccgccgg agtcggtaag
    20821 aatcggtcct ttccactgca taaaatcgtg catatcgccg tgcagtttca tgatttcctg
    20881 gcccgggcgc agccacaggt ggaaagtgtt gccgaggata atttgcgcgc cagtggcttc
    20941 aacttcttcc ggcgtcatcc cttttacggt gccgtaggtg ccaacaggca taaaacaagg
    21001 cgtttccact acgccacgat caaagaccag gcggccacgg cgtgcgcgac cgtcggtggt
    21061 gtccagttca aatttcattt tttctccact acgtcagaaa aacagtccaa cgtttaaacc
    21121 agcgccgcgg aattactccc cgacgcgctc attaattgcc tgcggattgt acgtgataaa
    21181 catcgcatca ccgtaactaa aaaagcgata tttctcttct accgccgctt tataggcatt
    21241 catggtgtgt tgataaccgg caaaggccga aaccagcata atcagcgtcg actctggcaa
    21301 gtggaagttc gtcaccagcg catcgaccac tttgtactgg aagcccggat agataaagat
    21361 ttgggtatcg tcaaagaacg gttcgataag atcgtttttt gccgcctgtg ccgcgctttc
    21421 cagcgaacgt actgaagtgg tgccaaccgc aatcacccgg ttaccgcgcg ctttcgccgc
    21481 cagtaccgcg tctaccacat cctgcggtac ttcagcgtat tccgagtgca tgatgtgatc
    21541 ttcaatggtg tcgacgcgca ccggctggaa ggtgcccgca ccaacgtgca acgtcacaaa
    21601 cgccatctcc acgcctttgg cgcgtaattt ttccagcaaa ggctcgtcaa aatgcagacc
    21661 agcggtcggg gctgcaaccg cgcccggttt ttcgctataa acggtttgat aaagttcgcg
    21721 gtcagcgtct tcgtccggac gatcgatata cggcggcagc ggcatatggc cgatgctgtt
    21781 gaggatatcc agtaccgagc gttcatcatt aaattcgact tcaaacagtg cgccgtggcg
    21841 cgcggtcatt gttgcgttga tactttcgtc atcgcccagc aacagttctg cgccaggttt
    21901 tggcgctttc gaggcgcgaa tatgcgcaag aatgcgtttg tcgtcgagca tccgctcaac
    21961 cagcacttca atcttaccgc cgctggcttt acgcccaaac agacgcgccg ggatcacgcg
    22021 ggtattatta aaaaccagaa gatcgccggg gttgagctta tcgagtaaat cggtgaaagt
    22081 accgtgcgtc agcgcacccg tcggcccgtc cagcgacagt aaacgacagc tactgcgttc
    22141 aggcatggga tagtgggcaa tcagggattc gggcaattca aaggagaaat cggtaacgcg
    22201 catgacactg actcagacta aaataagagt cgggtagtct agtgccgggg cgctctccct
    22261 gcaaccttta cccccctttg gatagaatat aatcgatgaa ttttttagct cacctgcatt
    22321 tagcccatct cgcggaaagc tcgctttctg gcaatttact ggctgatttc gtacgcggaa
    22381 atcctgaaga aagttttccg cccgacgtcg tggctggcat tcatatgcat cgacgtattg
    22441 actgacaatc tgccggaagt ccgcgaagca cgggagtggt ttcgtagtga aacgcgccgc
    22501 gttgcgccta ttacgctgga tgtcatgtgg gatcactttc tttcccgcca ctggtcgcag
    22561 ctgtcgccgg actttccact acaggaattt gtctgttatg cccgcgagca agtgatgacg
    22621 attttgccgg actcaccgcc acgttttatc aatctgaaca attacttgtg gtcagagcag
    22681 tggctggtgc gctatcgcga tatggatttc atccagaacg tgttaaacgg catggcaagc
    22741 cgccgcccac gtctggatgc cctgcgtgac tcctggtacg atttagacgc tcattatgac
    22801 gccctcgaaa cccgcttctg gcagttttat ccgcggatga tggcgcaggc gtcacacaag
    22861 gcgttataaa acgttagttc atccataccg tagccgaaat ggcaggcaaa gccagaatcc
    22921 cgtcagtcag ttgcccatgc ccttctttgc attgccattg cacggcattg agaaacggtg
    22981 acgcgggtag caccacttca ctggcctcgc cacggttgat tgccaccagt acacgttgct
    23041 gattcagcac gcggacaaat accactacgt tatcttccgc atacagcacc tgacagccgc
    23101 cacgacgcag cgcctgactt ttcttacgca gcacaatcat tcgccggtac agcgcgaata
    23161 acgccgtatc ctgcttttcc acctgccagg ggaacggttt acggcaagac ggatcgtttt
    23221 taccatccag tcccacttca tcaccgtaat aaatgcacgg tacaccaggc caggtgaaca
    23281 gccagaccac cgccagcggc aggcgcgcaa tatcccgacc gagcagcgtt ttaaatcgcg
    23341 cagtatcgtg gctatcgagc tgattaaaca tacgtaattg ttgttgatga gaaagccctg
    23401 cgcggtaatt atccatccag gccatacagg tttgggcgtc aatttgctgc ggatcataag
    23461 agatgtcggt attggcaaga aatccccaca acgggaatgt gaagccacga tagttcatgg
    23521 cagcatcttc cacatcggcc tgtaaccatt gccgtgcatc gccaaaatgt tcgccgacaa
    23581 tatacgcttc cgactgggtt tctttcgccg cttcggtgat cccggcaacg tgctgcatat
    23641 tattgcgcgc cccacccgcc tcccccagca tatgcaccac atccagccgc cagccgtcca
    23701 tattccacgg cgctttcagc cagtggcgga caatactgtc ttccccgcga taaatttcat
    23761 tcaccagact ttccgactga taatccagct tcggcaagct ggcatagcca agccagtcga
    23821 gcgccgtgcc atcatcacta aacaagtacc agtcgcgcca gggcgattca gagttgtgac
    23881 aagctccccc cgtgccaaga ttgtgcc
//
LOCUS       DAJVQH010000054        23501 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJVQH010000054 DAJVQH010000000
VERSION     DAJVQH010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.054"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(34..1398)
                     /gene="tdcG"
                     /locus_tag="OQM87_002191"
     CDS             complement(34..1398)
                     /gene="tdcG"
                     /locus_tag="OQM87_002191"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1508321.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALHRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1470..1859)
                     /locus_tag="OQM87_002192"
     CDS             complement(1470..1859)
                     /locus_tag="OQM87_002192"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCS1508322.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1873..4166)
                     /gene="tdcE"
                     /locus_tag="OQM87_002193"
                     /pseudo
     CDS             complement(1873..4166)
                     /gene="tdcE"
                     /locus_tag="OQM87_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            complement(4200..5408)
                     /gene="tdcD"
                     /locus_tag="OQM87_002194"
     CDS             complement(4200..5408)
                     /gene="tdcD"
                     /locus_tag="OQM87_002194"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCS1508323.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5434..6765)
                     /gene="tdcC"
                     /locus_tag="OQM87_002195"
     CDS             complement(5434..6765)
                     /gene="tdcC"
                     /locus_tag="OQM87_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCS1508324.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMAGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6787..7776)
                     /gene="tdcB"
                     /locus_tag="OQM87_002196"
     CDS             complement(6787..7776)
                     /gene="tdcB"
                     /locus_tag="OQM87_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCS1508325.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAILGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7875..8813)
                     /gene="tdcA"
                     /locus_tag="OQM87_002197"
     CDS             complement(7875..8813)
                     /gene="tdcA"
                     /locus_tag="OQM87_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCS1508326.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            9003..9347
                     /gene="tdcR"
                     /locus_tag="OQM87_002198"
     CDS             9003..9347
                     /gene="tdcR"
                     /locus_tag="OQM87_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCS1508327.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            9603..10142
                     /locus_tag="OQM87_002199"
     CDS             9603..10142
                     /locus_tag="OQM87_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508328.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            10164..11340
                     /locus_tag="OQM87_002200"
                     /pseudo
     CDS             10164..11340
                     /locus_tag="OQM87_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
     gene            complement(11950..12326)
                     /gene="rnpB"
                     /locus_tag="OQM87_002201"
     ncRNA           complement(11950..12326)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OQM87_002201"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(12359..13510)
                     /gene="garK"
                     /locus_tag="OQM87_002202"
     CDS             complement(12359..13510)
                     /gene="garK"
                     /locus_tag="OQM87_002202"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCS1508329.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHTWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(13607..14497)
                     /gene="garR"
                     /locus_tag="OQM87_002203"
     CDS             complement(13607..14497)
                     /gene="garR"
                     /locus_tag="OQM87_002203"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCS1508330.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(14527..15297)
                     /gene="garL"
                     /locus_tag="OQM87_002204"
     CDS             complement(14527..15297)
                     /gene="garL"
                     /locus_tag="OQM87_002204"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCS1508331.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(15313..16647)
                     /gene="garP"
                     /locus_tag="OQM87_002205"
     CDS             complement(15313..16647)
                     /gene="garP"
                     /locus_tag="OQM87_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCS1508332.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            17022..18593
                     /gene="garD"
                     /locus_tag="OQM87_002206"
     CDS             17022..18593
                     /gene="garD"
                     /locus_tag="OQM87_002206"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCS1508333.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(18645..19469)
                     /gene="agaR"
                     /locus_tag="OQM87_002207"
     CDS             complement(18645..19469)
                     /gene="agaR"
                     /locus_tag="OQM87_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCS1508334.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            19718..20518
                     /locus_tag="OQM87_002208"
                     /pseudo
     CDS             19718..20518
                     /locus_tag="OQM87_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
     gene            20518..20982
                     /gene="agaV"
                     /locus_tag="OQM87_002209"
     CDS             20518..20982
                     /gene="agaV"
                     /locus_tag="OQM87_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCS1508335.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            20993..21772
                     /locus_tag="OQM87_002210"
     CDS             20993..21772
                     /locus_tag="OQM87_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCS1508336.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            21762..22571
                     /locus_tag="OQM87_002211"
     CDS             21762..22571
                     /locus_tag="OQM87_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCS1508337.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            22658..23092
                     /locus_tag="OQM87_002212"
     CDS             22658..23092
                     /locus_tag="OQM87_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1508338.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            23089..>23501
                     /locus_tag="OQM87_002213"
     CDS             23089..>23501
                     /locus_tag="OQM87_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1508339.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         6122 a         5995 c         5544 g         5840 t
ORIGIN      
        1 ttgtgcaccc aaggatgaaa gctgacagca atgtcagccg cagaccactt taatggccag
       61 tcctccgcgt gatgtttcgc ggtatttatc gttcatatct ttgccggttt catacatcgt
      121 ctcgatcact ttatcgagtg aaacacgcgg tgccgaggtg cggcgcatcg ccatccgcgc
      181 ggcgtttact gctttcacgg cattaatggc attacgttca atgcacggga tttgtacctg
      241 tccggcaacc ggatcgcagg tcagcccaag gttatgctcc atcgcgattt ccgccgcatt
      301 gcatacctgc gccggactgc cgcccagtag ttcagttaac cctgccgctg ccattgaaca
      361 ggccacgcca atctccccct gacagccgac ctccgcgcca gagatggagg cgttcatttt
      421 atacagcgcg ccaatagccc ccgctgccag aaaatagcgg gcaattgacc gctcgtttac
      481 cggacgacgg aacttatcgt aataagccag cactgccgga ataatgccgc acgcaccgtt
      541 agtcggtgcc gttaccacgc gcccgccagc tgcgttttct tcactaaccg ccagcgcgta
      601 catgttgatc cagtcgatga cattcatcgg atcgttagag atgttatcgc tagaaaccag
      661 ctgacgatgc agcgctacgg cacgacgcgg cacattgagc ggaccaggca gcacgccttc
      721 agtgttcatc ccgcgttcaa taccgtcatg catcacttgc cagatacggg caaaccctgc
      781 gtcaatttcc gctttgctgc gcagcgccag ctcgttgtgc atcatcaggc cagatataga
      841 caggccgttg taatcacaca ttttcagcag ttcacctgct gagtggaaat cgtaaggtac
      901 ggacgtttcg acatcgtgcg acaggccgaa gtgttcttct tcgacaataa acccgccgcc
      961 gacagagtaa taggttttac ttaatagcgc ttcctgtccc ttccaggcag tgatccgcat
     1021 tccgttctca tggcgaggca acatttcggg atggaagata atgttctttg ctacaggaaa
     1081 atcaacaata tgcgcaccag atgccactgg cagccgcccg ctgcgcgtta ctaactctat
     1141 aaatgcaggg atctcatcaa tgacaacatc ctgcggactg tttcctgcca gtcccatgat
     1201 aatggcgaca tccgtggcat ggcctttgcc cgtcagtgac aacgacccgt acagatcgac
     1261 cacaatatgg ctcgtcgcgg ttaataagcc gctactttcc agccgatcaa taaaactttt
     1321 tccggcattc attggcccca cggtatgcga actggaggga ccaatcccaa ttttgaaaat
     1381 atcgaatgca ctaatcatat ccacaccctc ggattgccgt tcagtgaagt ggagcggaac
     1441 gaccttgcga ccgtcccgct cacgaggctt tacgcactac gtactgcgat agcttcaatt
     1501 tccagcttca catctttcgg caaacgcgca acctgcacac agctccgggt cgggtaggtc
     1561 gcctgatgct catcgaagaa ctgcttatag acttcattga tggtggcaaa atcattcaga
     1621 tcggtgatga acacggtcat cttgatgata tcgcccacgc tcagcccggc agcaaccacg
     1681 atcgctttga cgttttcgag gcttaaacgc gcctgatctt gcacatcagc cgggatctca
     1741 ccggtctgcg ggcaaaccgg aatttgcccg gaggtgaaga ccatgctgcc taaatcaacg
     1801 ccctgaacat aagggccgat tgcgcctagg gcacgttgcg tttcgataat ctttttcata
     1861 catcctccgg cgtcagagcg cctgggtaaa agtacgtgaa ataacatcct gttgctgttc
     1921 acgggtcagt gcgttgaagc gcacggcgta gccagagaca cggattgtca ggttaggata
     1981 tttttccggg tgctcgatgg catccagcag catttcccga ttcattacgt tgacgttgag
     2041 gtgttgaccg ccttcgacat ccgcttcgtg gtggaaatac ccatccagca ggccgacaag
     2101 gttggtttta cgtactggat cttctttgcc cagcgccgca ggaacgattg agaaggtgta
     2161 cgagatccca tctttggcgt aggtgaacgg cagtttcgcc accgacgtca atgaggccac
     2221 ggcacctttg cggtcacgac catgcatcgg gttagcgccc ggcgcgaacg gtgttccggc
     2281 gcgacgaccg tccggcgtat taccggtttt ctggccgtac accacgttag aagtgatagt
     2341 cagaatcgac tgggtaggga cggcgttgcg ataggttggc agcgctttaa ttttcttcat
     2401 aaagcgttca accaggtcgc aggcaatgct gtctacgcgc tcgtcgttgt tgccgtactg
     2461 cggatattca ccgtcgattt caaagtccac cgccaggccg ttttcgtcac ggattggttt
     2521 cacgcgggca tatttgatgg cagacaggga gtccgtcgcc accgacaggc ccgcgatgcc
     2581 gcatgccata gtgcgataga catcacgatc gtgcagcgcc atcagcgaag cttcgtagct
     2641 gtacttgtcg tgcatgtagt gaatgatatt cagcgcgctg atgtactgca ccgccagcca
     2701 gtccatgaag tgatcgaggc tgtccatcac tttgtcgtaa tccagcacgt cgtccatcag
     2761 cggtgctgtt ttcggcccga cctgaatctt cagcttctcg tccaccccgc cgttaattgc
     2821 gtagagcagc gttttcgcca ggttagcgcg tgcaccaaag aactgcattt gcttaccaat
     2881 caccattggg ctgacgcagc aggcaatcgc gtaatcgtcg ctgttgaagt cagtacgcat
     2941 cagatcgtca ttttcatact gcaaggaaga ggtgacgatc gacacctgcg cggcatattt
     3001 tttgaaggca atcggtaatt cttccgacca aagaatggtc aggttaggtt ccggtgccgg
     3061 ccccatagtg tgcaggtgtg caaatagcgg aaggagtttt tggtcaccag cgtacgaccg
     3121 tccagcccca tcccgccgat cacttccgtc gcccagattg ggtcgccgga gaacagcgaa
     3181 tcaaattccg gtgtacgcag gaagcgtacc atacggatct tcatgatgaa gtgatcgatc
     3241 agttcctgtg cctgctgctc attgaggacg ccagctttaa agtcgcgctc aatgtagata
     3301 tccaggaacg atgccgtgcg ccccagcgac atcgcgccgc cattttgcga tttcactgcc
     3361 gccagataag cgaagtagag ccactgcacc gcttcctgtg cattctgcgc cgggcgagag
     3421 atatcaaagc catatttcgc cgccatttcc tgaatctgca acagcgcatg acgatgctct
     3481 gccagctcct cacgcagacg gatggtggct tccagatcct cgcctttttc cagacgagac
     3541 tggagatcgg caaattgcag ttcgcgttca cgcaccagat aactgatgcc atacagcgct
     3601 acgcggcgat agtcaccgat aatgcgccca cgaccatagc catctggtaa accggtcagc
     3661 acgccagatt tacggcagcg cagcatatcc ggtgagtaaa catcaaatac gccctggtta
     3721 tgggttttac gcagatcggt aaacagatat tcaaattcac tgtccatttc acggccatag
     3781 gcgtggaatg aacttttaat catattaatg ccaccgaacg ggtgtagcgc acgtttcaac
     3841 ggcgcatccg tttgcaggcc aacaattttt tccagcggct ggttaatata tcccgcatca
     3901 tgagcggtaa ttgtggtggc aatattggta tcgaaatcaa ccggcgcgtg ggttgcgttt
     3961 tcgatacgga tgccttccat tactttttct cacaattccg tggtggcagg cgtcgcttcg
     4021 gcgagaaaag attcatcgcc ttcatacggt gtatagttat gttgaataaa atcgcggaca
     4081 ttaatttcgc ttttccagtc cgtaccttta aagccaagcc atgcgtcagc gtacagctta
     4141 tcgctggtat caatatctac cttcatgaaa aataatctct ctacaatact tcaactaaat
     4201 tatgcaaatt ctgcgggcgc gttaacttta cctaaatgaa tggcatccaa agcaatcatt
     4261 ttttcttcgt tagtcggaat aacgacgcaa atgacatgcg cattttcact ggaaacaatt
     4321 cgctcgccaa aagagttaga tcgattattc atttctgtat caatctctac gcctaataca
     4381 gccaaatgtt ccatgaccag acgacgaatt aaacttgaat tctctcctat tccgccggtg
     4441 aatataattc catccagccg acgtaatgaa gctgcatgtc cggcaatatg acgggcaatt
     4501 cggtgaacaa aggttttaat tgccagttgc gcgcgttcgt gaccttcatg ccaggctttt
     4561 tccagaacac gtaaatccga agaaagaccg gaaataccta ataatcccga ctctttattc
     4621 actacgcgtt ccaggtcacc caggctctgg ttggtttggc tggcgaccca ggacatcgca
     4681 ccaaagtcga catcgccact gcgggtaccc atcatcaagc cttccagcgg cgtcattccc
     4741 attgaggtgt caacactctg accgttgcga actgcgcaga ttgacgcgcc attgccaaga
     4801 tgcgccacaa ccaggccgga gtcatcttcc gccagattca gcagcgaatg tgcgcgctgg
     4861 gaaacatagc ggtgcgacgt gccgtggaaa ccataacggc gtacacctaa ctcttcataa
     4921 tatttccacg gcaggccgta taaataagct tccggagcca tcgtctggtg gaaactggta
     4981 tcaaatacgg ccacctgagt tacgcccgga aataattgct gcgccgattc aataccactt
     5041 aaatttgcgt aattatgcag gggtgccagt ggagaaacgc gacggatatt atcaatgact
     5101 tcatcggtaa taatggcgga ctcggtaaaa atactgccgc cgtgagcgat gcggtggcca
     5161 attaaggcca cgctgtcatt taaattccgt ttttccagtt caaatgcaat tgccttcaat
     5221 gcaccttcgt agctgtggag agccagcggt gctggctctc ccccatttac ggataagaat
     5281 gcattttccg agttaatacc gtcggcaata cctgacatta atacttcaca gtcgctggca
     5341 tcgagtacgg aaaacttaat cgaagacgaa ccacagttaa taaccaaaac aaccggaaat
     5401 tcattcatct cttttctcat cctgagttac ggattaaaac agtttgtata cgatgttcag
     5461 gatggtcagc agaccaatca cggtaacaaa cacgttatcc agacgaccac ggtatttcgc
     5521 cagagacggc gctttacgga tagcatacat cggcaacagg cacagcaggg atgcgataat
     5581 cggtgcgccc atggcttcaa tcaggtcgag gatgttcggg ttggcgtagg caacaaccca
     5641 ggtggagccc atgatgaaga tcatgctgag agtattcagt ttacccagcg acactttggt
     5701 tttgtcacct ttataaccga acttcagaat cagaccattc aagccttcca gcgtccccag
     5761 atagtgaccg aagaaagatt tgaagatagc cacgagtgcg atgatggaag ccgcatattc
     5821 cagtgtaatc gcgaacgttg ttttcgtacc ggccatggac gcaaagtggt tagccagata
     5881 agaaagcact ggaatattct gcactttggc ttccgccatg ttggccggag acagagtaaa
     5941 caggcagcta aaggcaaaga acatcaccac tgcaaccatc agcatgctgg cacgagaaat
     6001 gatttgggaa catttacgtt cggtgaagtc gcgaccgaag tctttctcat actcttcacg
     6061 tttagaaacc acgaaggaag agacgattgg cgagaagtta aaggagaaaa ccatgatgga
     6121 aatccccagc cagacagtga tcaggatacc gtcatgaccg gttaacgaca gcgaaccgag
     6181 gtcaacctgg tcgataactg cagagttcca gtaagggatc agcgacagag aaatcagcac
     6241 caggctggcg ataaacggcc ataccaggta gctcatcact ttaaccatca gatccttacc
     6301 aaaccagatg acgaaagcca tcagcagcaa caggaacagc gccacaaagc cgcgattcag
     6361 cggtgcaaat ccgagctggt tttcccagaa cgtcataaag gtattggtaa tagtaacgcc
     6421 ataaatccac agcagtgggc aaatcgcgaa gaagtacagg aacgtgataa ccacgccgcc
     6481 agttttacca aaatgctctt ccaccgtttc cgtaatgttg ccggaagggt tagagccaga
     6541 aagacacaga cgcgccagcg cccggtggca ataaaatgcg atggggtatg ccaataccaa
     6601 catcagaaga atcgggatca gtccgccaaa acctgcgcgg atagggaaga acagcacccc
     6661 ggcgccgatt gccgtaccaa acaagcctaa cgtccatgtg gtatctgatt tacgccagga
     6721 cgattgtttt gtctggctgg atacaatgct atctgaagta ctcatatcct atcctcaacg
     6781 aattaattaa gcgtcaacaa aaccggtgat ttgagagacg cgagaaagat cgatattgcc
     6841 gccggaaata atactgacgg tttttctgtt ttgaatatat tggtctaatt taccgcttaa
     6901 taatgcagca catgccagag cgcctgcgcc ttcggtgacg actttattgc gctgaattaa
     6961 ggcaatcata ctgtttctga tttcgtcttc gctgaccagc acgatgtcat cgactaattc
     7021 acgaacgatt tcgtaagtta aattacccgg gcgggagaca tcacaaccat ccgccagggt
     7081 gccggtagtt cggtgcgtgg ttatttctcc ggagtggaaa gaagccgcca tgccgtgaac
     7141 gttttctgac tgtacgccaa taacacgaat ggtcgggtta atagatttaa ttgccaccgc
     7201 aataccagca attaaaccac caccaccaat tggcacaatc acgttatcga catcatagag
     7261 atcttccata atttccagac caatcgttcc ctggccagca atcactttcg gatcatcgta
     7321 aggtgggata aaaatacgac cttccatttc gacaatttcg ctcactttag cgatagtgtc
     7381 gttgaagtta tcaccatgca gaacgacttc tgcggagtag tcgcacgttg ccgctacttt
     7441 ggattttggc gcaccttttg gcatcaccac tttaccgtcg atacccagta tcgcgcagga
     7501 gagcgaaacc ccttgcgcat ggttgcccgc agaacaggcc accactcctt tgcgtttttc
     7561 cgcatcggtc agtgaactta atttattaaa tgcgccacga attttaaatg aacccgtacg
     7621 ctgcatattt tcaaacttca ggaatatttc acctttgcaa cgttcactaa aatagttgga
     7681 gcgaggcatg cctgttttat aaattcgccc agccagtcgt tgtttcgctt caataatatc
     7741 atcaatagca accggcagat cgtatgtaat atgcattata aaacctcttc gcctgaatta
     7801 aatatgtagg taaccgtaac cgacacctgc aaaacagaca ggtggattat ttatgtttaa
     7861 ataaaaacaa ataactaacc aacttctatt aattgccttc gtctacaccc attataagat
     7921 gaatactctt tggctaattc caccaaaacc gatgctgctt ttttaatacg ataatttttc
     7981 gaccataccg cggcatattg tgccacaggt aatgtttctt caaccggaat agtaataaat
     8041 tgattagaac caaaaggtga cgtcatatca caaggaatta cagttaagaa atcagcattg
     8101 agaacaagat tataaattgt cacgactgag tcggttttaa cgatgttttc aatactgatg
     8161 ccatttcttt gtaacgtagt aagcagttcg ctgtagtacc ccatattagt ttgtggcaac
     8221 acccactgtt cgttcttcaa cgactccagc gtggtggtgc cggtgcatgt tcgggactta
     8281 ctggctacca gcacaaactc ggactcgaac agcggctcaa catgtaaatc ctgaagcttc
     8341 atttctgcac ttaacgtacc aatcgcaaaa tccagtcgac cgtcgcggat tgccggtaag
     8401 aacgaagaca gttgcgcttc atacatagaa acctgcgctt tcgggaacac ctctttgaac
     8461 ttgttgatca tccctgacat aaaagtaaaa ccaatcaatg aaggaaaacc aaatgagact
     8521 tccaccaccg cctcagaaga cataccgctt atctcattaa ccatattttt catttcacgg
     8581 gtaatggatt cggaacggga gagtaacaat tgaccggcag gtgttaatgt tacaccggta
     8641 tttttccgca ccactaattc cacaccaaaa taatcttcaa tatcgttaat gattttactg
     8701 acggccggtt gagttaaccc taattctttt gcagccgagc cgatagagcc acttctaatg
     8761 acttcctgaa agactaccag gtgctgcgtt ttcggaagaa gaatagtgct cataacgacc
     8821 tacgttaatt acctcattga cggcatgaag tgtatcaaaa tgaaataaac aggatatgtg
     8881 cgaccactca caaattaact ttcattactt tccaaggtat cgttattaca attaaatacc
     8941 tttaaatatc aacaagttaa agcataaaaa tcagcataaa ccctgatttc tatcaaaaaa
     9001 aaatgacggg gataaccata ttttatggcg ataacatcat tcgttatgtg gttaatacaa
     9061 aaaaggggct gagaccatat ttcaagcaat taccggataa ttaccaggca aaatttgagt
     9121 taaatttaat gagcaagttc tcaaatttta ttataaataa accattttca gcgataaata
     9181 ctgcggcacg tcacattttt tcacgctatt tgttggagaa caaacattta ttttatcaat
     9241 attttaaaat ttcgaataca ggtattgatc atctcgaaca attgattaac gtcaactttt
     9301 tctcttctga caggacgtca ttttgtgaat gcaatcgttt tccataaatt cttctcccct
     9361 cataggcgac gaatagcatt ttgtgttgag gatcacaaaa cgaataattg ctggtcgccg
     9421 cgataaggtc agacgaagtc aacaaggggg attttcactg tgctttacat cagcgtagga
     9481 cacagaatgt tgcttcgttt actgtaacgc cgggtaaatg agcgtttttt gatagtgcgt
     9541 aagtgtcgtg cgaggaaaat aagcatctat tattgtttgt catgacgaca aggcaggcat
     9601 caatgaaagg cttcccaatt gcacatattt ttcacccttc aatcccgcca atgcacgcag
     9661 tggttaacaa tcacaataga aatattgatt attggacggt aaaaagaaag tttgcagaaa
     9721 ttgtctccac caatgacgtt aataaaattt acagtataag taatgaactg cggagagtat
     9781 tatctgcaat tactgcattg aatttctatc aaggcgatgt tccttctgtc atgatccgaa
     9841 tccaaccgga aaatatgagt ccattcatca tagatatttc tacaggagaa catgatgatt
     9901 atatcataca aacattagat gtaggcactt ttgcaccttt tggtgaacaa tgtacttgct
     9961 cagccgtcaa taaaaaagag ctggaatgta ttaaggagac gatttctaag tattgcgcaa
    10021 agttcaccca aaaagaagcc atcttaacac cccctaggca ctttaataaa acctgcatta
    10081 cttcagattg ttggcaaatt ctcttttttt cacccgatca ttttaataat gatttttatt
    10141 gattatttat aaaggataaa ataatgttcc ctgtttcatc aattggtaat gatattagta
    10201 gtgatttagt ccgtcgaaaa atgaatgacc ttcctgaaag ccccatagga aataacctcg
    10261 aagcactagc acccggtata gaaaaactaa aacagacctc tattcaaatg gtcactttac
    10321 ttaacgcgtt acaaccaagt ggaaaatgca ttatcactgg tgattttcaa aaagaattag
    10381 cgtacttaca aaatgtaatt ctttataatg actcgtctct tcgtctggat tttttaggtt
    10441 ataacgccaa aattattcaa cgatcggaca atacctgcga acttaccatt aatgaaccgt
    10501 taaaaaacca ggaaatatct acaggcaata tcaatgttaa ttgcccatta aaagatattt
    10561 acaatgaaat caggaggttg aacgtaatct ttagttgtgg gactggagat atagttgatc
    10621 tatcctctct ggacttacgt aatgtcgatt tagattatta tgatttcaca gataaacata
    10681 tggctaatac tattctaaat cattttaaat tggattcaac aaattttact aatgccaaca
    10741 tgtttcaggt taattttgtt agttcaacac aaaacgccac aatctcctgg gattatttac
    10801 taaaaataac gcctgtttta ataagcatta gcgatatgta ttctgaagaa aaaatcaagt
    10861 ttgtcgaaag ttgtttaaat gagcttggag acattaccga agaacaatta aaaattatga
    10921 gatttgcaat tataaaatct ataccaaggg caactcttac agataaatta gaaaatgaat
    10981 taacaaaaga aatatataaa agctcatcga aaatcaacaa ttgcttgaac agaattaaat
    11041 taccagagat gaaagaattc tcatcaaaaa aatatatgat tacatcgata caatcattga
    11101 agattatgaa aatatcaaag aaaatgctta tctggtcgtc ccccaaatta attatactat
    11161 ggatttaaac atagaagact ctagctcaga agagttaata ccctcgagaa agacgaaaat
    11221 tctccggaca atggctttga ggtcggggaa tataacacat atgaagcata taactcagag
    11281 aatcaatatt ttaccagaga ggactatacg tataattacg accttttaaa tgcaatatag
    11341 acttaataac ttcttacaaa gtctattatt aataccgtag atattcatct taccctgtgc
    11401 accaaaagca aaaacttatc gacgcgggtt tgaaactgat ggcaaccgca aaaatgcctg
    11461 atgcgctacg cttatcaggc ctacgccatc tctgcaatat attgaatttg cgtgcttttg
    11521 taggcaggat aaggcgttca cgcagcatcc ggcatgaata aagcgcactc aacaatctga
    11581 aacccgccgg aacggtttat tacgtacatc aggtgaaact gaccgataag ccgctttctt
    11641 ttgggtatag tgtcgtggac agtcattcat ctttctgccc ctccaaaagc aaaaacccgc
    11701 cgaagcgggt ttttacgtat atcaggtgaa actgaccgat aagccgcttt cttttgggta
    11761 tagcgtcgtg gacagtcatt catctttctg cccctccaaa agcaaaaacc cgccgaagcg
    11821 ggtttttacg tacatcaggt gaaactgacc aataagccgc tttcttttgg gtatagcgtc
    11881 gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa gcgggttttt
    11941 acgtaaaaca ggtgaaactg accgataagc cgggttctgt cgtggacagt cattcatcta
    12001 ggccagcaat cgctcactgg ctcaagcagc ctacccgggt tcagtacggg ccgtacctta
    12061 tgaaccccta tttggccttg ctccgggtgg agtttaccgt gccacggact gttaccagcc
    12121 gcgcggtgcg ctcttaccgc accctttcac ccttacctga tcccgcttgc acgggccatc
    12181 ggcggtttgc tctctgttgc actggtcgtg ggtttccccc ccaggcgtta cctggcaccc
    12241 tgccctatgg agcccggact ttcctcccct ccgcccgtct cccccgaaga ggacgacgac
    12301 aaagcggcga ctgtctggtc agcttcggcg cgcagtatag agggtttgcg cgcccttgtc
    12361 accccgtgtt gcgcattcca atcgccagtg tcgcggcaat attacgtgaa gcacggcaga
    12421 tattgtcata agccccgcgg aatgcttcgt ccaacgtacc tatgctggtc aatacgctga
    12481 agaccgcatc aatgccatgc tgatgtacaa cgccaacatc atcggtcagg ctacccgcac
    12541 ccgcaatgcc aataaccggt ttatggtact tcttcgccac gtttgcgacg ccaatcggta
    12601 ccttgccgtg aatactctgg ctgtcaatac gcccttcacc ggtgatcacc agcgtacaat
    12661 cgtgaatatg ttcctccaga ttcagcgccg tagtgacgat ttcaatacca cttttcagtt
    12721 ccgcaccaag aaacgccatc agcgccgcac ccataccgcc cgcagctcct gcgccgggga
    12781 catcttttac atcaacatgc agcgcttttt taatgacatc ggcatagtga gagaggttat
    12841 tgtccagctc aacaatcatc gcttcactgg ctcccttttg tgggccaaag atgcgcgatg
    12901 cgccgttatc gcccaccagc ggattggtga catcacaagc gacgcgaatg acgcaatctt
    12961 ttaagcgcgg atcgaggccg gaaatatcaa tatcattcag agtattaaga ctaccgccgc
    13021 caaaaccaat ttcattgccg ttggcgtcgc ataatttcgc ccccagcgcc tgtaccatgc
    13081 ctgcgccgcc atcatttgta gcgctaccgc caatgccgat aataatgttt gtcgcgccgc
    13141 tctccagcgc ctgcaggatt aactcgcctg tgccgcgtga agtggtcacg agtggatcgc
    13201 gtttttccgc aggtaccagc tccagcccac tggccgccgc catttcaata aacgcggttt
    13261 tgccatcgcc ggagatcccc caactggcat tcactttctc gcccagcggc cctgtaaccc
    13321 aggtgtgacg ttcagaaccc tgggtggctg caatcatcgc ttccaccgtt ccttcgccac
    13381 cgtcggcaac cggaatagaa acgtactgtg catcaggaaa aatttcccga aatccttttt
    13441 ctatcgcctg cgcaacctcg ctggcagata aactttcttt ataagagtct ggggcgatta
    13501 cgattttcat acctatgcct gttaccacat gacgccggag ggcgtttctc ttattcggcc
    13561 tggattccag gcccggattg caatacgcca tccgggcacg acgtcattaa cgagtaactt
    13621 cgactttcgc cagtttttcg tagtagcacg ccagggcgct atgatccgcc gttcctaaac
    13681 catctgctcg cagtgcctgc atcatctcca taaccgcagc tgtgagcggc agttgcgcgc
    13741 cgacgccgtg ggaagtatcc agcgcatttg ccagatcctt aatatgcaga tcaatacgga
    13801 agcccggctt gaagttgcgg tccatcacca tcggcgcttt ggcatccagc acggtactgc
    13861 ccgccagtcc accgcgaatt gcctgataca ccaggtccgg gttaacgccc gctttagttg
    13921 ccagcgttaa cgcttctgac atcgcggcaa tattcagcgc cacaatgacc tgattcgcca
    13981 gtttggtgac gttacctgca ccaattgccc cggtatgcac cacggaaccc gccattgctt
    14041 tcatcaaatc atagtatttg tcgaaaatag ccttgtcgcc gcccaccatc actgacagcg
    14101 taccgtcgat ggctttcggt tcaccgccgc tcaccggagc atccagcata tcaatgcctt
    14161 tcgctttcag cgcttcgctg atttcacggc ttgccagcgg tgcgatagaa ctcatatcga
    14221 tcaataccgt acctggcttc gcgccttcaa taatgccatt ctcacccagc gccacctctt
    14281 tcacatgagg ggagtttggc agcatggtta tgatgacgtc gcactgttca gcgatcgctt
    14341 tagccgtaga ggctgtttct gcacctgcag caatcacgtc agcaatagct tctgggttac
    14401 ggtcagcaac caccagcgag taacctgctt tcagaaggtt tttactcatt ggtttaccca
    14461 taatccctag gccaataaaa ccaactttca tagtcatatc aatcatctct cttgttgcgg
    14521 tggtggttat tttttaaagg tatcagccag tttatgagtg gcagagcgga agacgccgag
    14581 atcgctgccg acagccacaa acgtcgcgcc ccattccaga taacgacgcg catcggcttc
    14641 gaccggcgcg aggataccgc tgggtttgcc gtgcgcgctg gcacggttaa aaatgtgctg
    14701 aattgctttt tgtacatccg ggtgtgatgc attgccgaga tggcctaatg ccgcggccag
    14761 atcgctgggg ccgacgaaga tgccgtctac gccttcggta gcggcaatgg catcgacgtt
    14821 atctacgccc tgctgacttt ctatctggac cagaatagtg atgttcttgt tcgactgagc
    14881 gaaataatcc gccacggtgc caaacatatt ggcgcggtga gaaacggaga cgccgcgaat
    14941 gccttccggt gggtaacggg ttgatgccac cgcctgctct gcttcctctt ttgtttctac
    15001 aaaaggaatc aggaagttat agaaaccgat atccagaaga cgcttaataa ttaccggctc
    15061 gttggtcggc actcgcacta ctggcgcgct ggcgctgcct ttcaaggcca ttaactgcgg
    15121 aataaacgtg gagatatcgt ttggcgcatg ttcgccatcc agcaccagcc agtcaaaccc
    15181 agccaaacca agaacttcag tgctaatcgg gttagagagt gctgaccagc aaccaatttg
    15241 tacctgtttc gcagccagtg cggctttgaa tttattcggg aaaacatcgt tattcatcgc
    15301 ttataccttt gcttatttct gcaattccat acgtttaatg tcgccaacta cgaagaggta
    15361 gcagaccatc gccatcagcg ctgaacatcc cacgaaaacc agtgctgcat tgaaggagtg
    15421 cagttcactt accaggtagc caatcaccag tggagtgaca atggaggcaa cattgccaaa
    15481 gacgttaaag acgccgccac agaggccaac aatctctttc ggcgcggtgt cagaaatcac
    15541 cggccagccc agagcaccaa atcctttgcc aaaaaaagcc agcgccatca gcatgaccac
    15601 cagcgtggtg ttgttggtgt agttacataa gatgatggtg gaagccagca acattcccag
    15661 cacaatcggt agcttacgtg ccagggtcag ggataaaccg cgtttgatca gataatccga
    15721 gaagacacct cccagcacgc cgcccgcaaa accacacagt gccggaatcg aggcgaccag
    15781 acccactttc agaatcgaca tgcctttttc ctgcaccaga taaatcggga accaggtgag
    15841 gaagaaccag gtgatggtgt tgataaaata ttgtccgaaa aatacgccca acatcatgcg
    15901 gttagagagc aattgcttga tgtaatgcag tttgggaccg cttgctgccg cgctgcccgg
    15961 ctttttgtgg tccatatcga ccaccgcgcc attttcagag ataaacttca gctcttcctc
    16021 agacatacgt gggtgatctg tcgggttatg aatcaacttg atccacagcg ccgtcagcac
    16081 aaaaccaatc acccccataa cggtaaagac gtgctcccat ccccaggcga aagtcagcca
    16141 gccaagcaga ggcgaaaaga gcgccagcga gaaatattgt gccgagttaa agatggcgga
    16201 ggcagtacca cgttctttcg tcgggaacca ggcggcgaca atacgggcgt tcgccgggaa
    16261 tgatggagct tccgagaatc ccagcataaa gcgcataaag aacatggaga tccctgccca
    16321 ggccagcggg aacatatcaa caaagccttg caggaaggtg aacagcgacc agaaaaagag
    16381 gctgtaggtg taaacttttt tcgagccaaa cttatcaagc aaccagccgc cggggatttg
    16441 catcagcaag taggcccagc caaaagcgga gaagatgtaa cccatcgaaa ccgcacttaa
    16501 ctgcaactct tttgccactt cggtaccagc aatagacagc gttgcacgat cggcgtagtt
    16561 aacggcggta acaataaaaa taatcagtaa tattaaatag cgggtatgca cacctttcct
    16621 tttttcgtca acggtgtcca gaatcatttt atttacctcg ggtacttatg ctgattttta
    16681 ttattatggg gaaggtgtta tttataagtt tcatttatgc cgtaacgaca atgaactcgg
    16741 gaattagtat aagcagcgcg agaataataa tcattgtgca aatgctaatt taattaatac
    16801 tttttaaata ttattttgag catatgcaca taaggttgcg cgctaaagca cagatttgcg
    16861 ctttaccttg ccgggcggca ctgcaatccc tgaaatgatt gacattgatc acatttctgc
    16921 gtttaaactc ctgacattct tatttcaccc aatgaagtca tttattttta aatgagacca
    16981 ggtcctcatt ttaataaccc ctggctggag aatattgcac aatggccaac atcgaaatca
    17041 gacaagaaac gccaactgcg ttttatataa aagttcacga cacagataat gtggcaatta
    17101 ttgttaatga taatggcctg aaagcaggaa cgcgttttcc ggatgggctg gaattaattg
    17161 aacatattcc ccaggggcat aaagtcgcat tgctggacat tccggctaat ggtgaaatta
    17221 ttcgttatgg cgaagtgatt ggttacgccg tgcgtgcaat cccacgcgga agctggatcg
    17281 acgaatcaat ggttgtacta ccggaagcgc cgccgttaca cacgctgcca ctggcaacca
    17341 aagtcccgga acccttaccg ccgctggaag gatacacctt tgagggctat cgcaatgccg
    17401 atggcagcgt gggcaccaaa aacctgctcg gcatcaccac cagcgtccac tgtgtggcag
    17461 gcgtggtgga ctatgtagta aaaatcattg aacgcgatct gctaccgaaa tacccgaacg
    17521 tcgatggcgt ggtggggctg aatcatttgt acggttgtgg cgtggcgatt aacgcaccgg
    17581 cggcagttgt acctattcgt accattcaca atatttcgct gaaccctaac tttggcggcg
    17641 aagtaatggt gattggcctg ggttgtgaaa agttgcagcc tgagcgcctg ctgactggaa
    17701 cggatgatgt gcaagctatt ccagtagaaa gcgccagcat tgtcagtttg caggatgaaa
    17761 agcatgtcgg ttttcagtcc atggtcgagg atattttgca ggtcgccgaa cgccatctac
    17821 aaaaactgaa tcaacgtcag cgagaaacct gtccggcttc agaactggtt gtcggcatgc
    17881 agtgcggtgg cagcgatgcg ttttctggcg taacggcaaa cccggcggtt ggctatgcgt
    17941 ctgatctact ggtgcgctgc ggcgcaacgg tgatgttttc agaagtcacg gaagtgcgtg
    18001 acgcgatcca tctgctgaca ccacgcgcag tgaacgagga ggtcggcaaa cggctgctgg
    18061 aggagatgga gtggtacgat aactatctca atatgggaaa aaccgaccgc agcgccaacc
    18121 cttcgccggg caacaagaaa ggcggtctgg caaacgtggt agagaaagcg ctcggctcca
    18181 ttgctaaatc gggcaaaagt gcaattgttg aagtgctgtc gcccggtcaa cgcccgacta
    18241 aacgcggatt aatttacgcc gcgacgccag ccagcgattt tgtctgtggc acgcaacagg
    18301 tggcttcggg tatcaccgtg caagtgttta cgaccggtcg tggtacgccg tacggcctga
    18361 tggcggtacc cgtcattaaa atggcaaccc gcaccgagct ggcgaaccgc tggtttgatt
    18421 taatggatat taatgcgggc accatcgcta ccggcgaaga aactattgaa gaggtgggct
    18481 ggaagttgtt ccactttatt ctcgacgtcg ccagcgggaa gaagaaaacc ttctcggatc
    18541 aatggggact gcataaccag ctggcggtgt ttaacccagc accggtgacc tgataattca
    18601 attcgtatta tgcccgatag cacacgttta tcgggcatat tgagtcacag cgaagacgcg
    18661 tgatccccga ccagaatcac ttcaacccca gcctttcgca gtccttccag actatccgca
    18721 ggaatgcctt catcaacaat gatcatgtcg atacgttgag tatcaatgat cttatgtaaa
    18781 ctggaacgat tgaatttact ggaatcggtg accacgatga tccgttccgc aacttcgcac
    18841 atccgacggt ttaaacgggc ttcatcttca ttatgtgtgc tgacgccgcg ctccagatcg
    18901 atcgcatcta caccaagaaa cagcatatcg aagtggtaat tttgcagcga ttgctcagcc
    18961 tgatcgccgt aaaaagattg cgactgacgg cgcaaatgcc cgccggtcat cagcagctca
    19021 acgccttccg cttccagcaa cgcattagcc acgttcatac cgttggtcat cgcaattacg
    19081 tcagtgtact tgcgcatcag acgagcaatc tcaaaagtgg tggtcccgga atcgaggatc
    19141 acccgatgac ctggctgaat caactcaacg gcagctttcg caacgctgcg tttcatcgcg
    19201 gtgttcagtg cgcttttatc ttccactgat ggctcgactg acggcgtcgt gctatcgcag
    19261 atcaacgcgc caccataggc acgcacagcg atcccctgct tttccagaaa agccagatca
    19321 ttgcggatcg tcacagtaga tacgccatac aatgccgaca gatcgttaac ctgcacactc
    19381 ccttgctgtc gcagacgctg aatgatctgt tctcgtcgct cgctggtgcc tgtcactcgc
    19441 ttctcacctg aagcgtcggt attactcata gtaagtcctt tcgtaaaact ttcgtttcat
    19501 ttcgttttgc ctattaacgc ctttctatta agcaaatgca agcccatctt gcccattggc
    19561 gcaagctact ctcgtttcag tgactttcat tatgtttctt ttgtgaatca gatcagaaaa
    19621 ctattatatt tcgttttatt tttatctcac catgatgcag tattaattga aacaaaacga
    19681 aagattaata tcgcagtaat ctgaactgga gaggaaagtg aaacatctga cagaaatggt
    19741 gagacagcac aaagcgggca aaacaaatgc aatttatgcc gtttgttccg cacatccgct
    19801 ggtgctggag gctgcaatcc gctacgccag tgcaaaccaa acgccgttac tgattgaagc
    19861 aacctccaat caggtagacc agttcggcgg ttataccgga atgacgcccg ccgattttcg
    19921 cggctttgtt tgtcagctcg ccgactcgtt gaatttcccg caggatgcgc tgattctggg
    19981 tggtgaccat ctggggccaa accgctggca aaacctgcca gccgctcagg caatggccaa
    20041 tgccgatgat ttgattaaaa gctacgttgc ggcaggattc aaaaaaatcc accttgattg
    20101 cagcatgtcc tgtcaggacg atccaattcc cttaactgat gacatcgtgg ctgaacgcgc
    20161 cgcccgtctg gcgaaagtgg cggaagaaac ctgtcgcgaa cactttggcg aagccgatct
    20221 ggagtatgtc attggtaccg aagtgccggt acctggcggc gcgcatgaaa ccttaagcga
    20281 gctggcggtc accacgccgg atgccgcccg cgccacgctg gaagcccatc gtcacgcttt
    20341 tgaaaagcaa ggtttgaatg ccatctggcc acgcatcatt gccctggtgg ttcaacccgg
    20401 cgtcgaattc gatcacacca acgttattga ttatcagccc gccaaagcga gcgccttaag
    20461 ccagatggtc gaaaactacg aaacgctgat tttcgaagcg cactctaccg attatcaatt
    20521 gttttaagcc ggattgatga acgcttgatt cacggtcagg tcggcgttca gtgggtcgga
    20581 tttgcggggg caaatctggt gctggtagcc aacgatgagg ttgccgaaga tccggtacaa
    20641 caaaacctga tggaaatggt actggcagaa gggatcgccg tacgtttctg gacgctgcaa
    20701 aaagttatcg acaacattca tcgcgccgcc gatcgacaga aaatcctgct ggtttgtaaa
    20761 acacccgccg atttcctgac gctggtgaaa ggtggcgttc cggtgaatcg cattaacgtt
    20821 ggcaatatgc actacgccaa tggcaaacaa caaatcgcca aaacggtttc tgtggatgcg
    20881 ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg tggaatgctt cgttcagggc
    20941 gtcccgacag agcctgctgt ggacctcttt aaattacttt gagggattca tcatggaaat
    21001 cagcctgttg caggcatttg cgttgggcat tatcgccttt atcgctggcc tagatatgtt
    21061 taacggctta acccatatgc accgcccggt ggtcctcggc ccgttggtcg ggctggtact
    21121 tggcgatctg cataccggaa ttttaaccgg cggtacgctg gaactagtgt ggatggggct
    21181 ggccccgctg gcgggcgcac agccgcctaa cgtgattatc ggtactatcg tcggcacggc
    21241 gtttgccatt actactggcg tgaaacccga tgtcgcggtc ggtgtcgccg tacctttcgc
    21301 tgtcgcagta cagatgggga ttaccttcct gttctcggtg atgtctggcg tgatgtctcg
    21361 ctgcgaccgg atggcggaga acgccgatac tcgcggcatt gaacgcgtga actatctggc
    21421 actgctggca ctcggcactt tctattttct ctgcgctttc ctgcctatct acttcggcac
    21481 ggaacatgca aaaaccatca ttgatgtcct gccgcaacga ttaatcgacg gcctcggcgt
    21541 cgcgggcggc atcatgccag caatcggttt tgccgtgctg ctgaaaatca tgatgaaaaa
    21601 cgtctacata ccctacttca tcctgggctt tgttgccgca gcctggctca agttaccggt
    21661 gctggctatt gctgccgccg cgctggcgat ggcgctgatc gatctgctgc gtaaatctcc
    21721 tgaaccgact caacctgcgg cacagaaaga ggaatttgaa gatggcatct aatcaaacca
    21781 ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac tggcgtcaat gaaaacgtgt
    21841 atgaagatcg gagcattgcc gcggagctga cgaaaaaaga tatcaaccgc gtcgcctggc
    21901 gttccatgct gttacaagcg tcttttaact acgaacgtat gcaggcttct ggctggttgt
    21961 acggtctgct gcctgctctg aaaaagatcc acactaataa acgcgacctg gcgcgcgcca
    22021 tgaaggggca tatgggtttc ttcaataccc atccgtttct ggtgacgttt gttatcggca
    22081 ttatccttgc gatggagcgt tctaagcagg acgttaacag tattcagagc accaaaattg
    22141 ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt ctggctaacg ctactgccga
    22201 tttgtggcgg gataggcgca agcctcgcgc tacaaggctc tattcttggc gcagtcgtct
    22261 ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt tggtctggcg cattatgctt
    22321 accgcatggg cgtggcggcg attcccctta ttaaagcgaa taccaaaaaa gtcggccatg
    22381 ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt ggcaacctat gttcgtttaa
    22441 gcaccacgct ggaaatcacg gcgggcgacg cagtggttaa gttacaggct gatgttatcg
    22501 acaaactgat gcctgccttc ttaccgctgg tctacaccct gaccatgttc tggctggtac
    22561 gccgcggcta gagtccgctg cgcctgattg cggttaccgt ggttctaggg atcgtcggta
    22621 aattctgcca tttcctttaa atacaatgag gtattagatg ttaagtatta ttttgacagg
    22681 gcatggcggg tttgccagtg gcatggaaaa agcaatgaag caaatcctcg gcgatcaatc
    22741 gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc gcgctgctta catcgcagct
    22801 tgaagaggcc atcgcccaac tggattgtga agacggcatt gtttttctga cggatttgct
    22861 gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg caaaaaccgg gctgtgaagt
    22921 gattaccggc accaatttgc aactgctact ggagatggtg ctggagcgcg aagggttaag
    22981 cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac cgagggctga ccagtctggt
    23041 ggacgagtta ggccgctgcc atgaagaata tccggtcgag gaaggaatat gacacacgtt
    23101 ctgcgcgcca gaaggctgct gactgaagag ggatggttcg atgaccatca gttgcgtgtt
    23161 gctgacggtg tcatcgcagc aatcgaaccg attccagtgg gcgtgactga acgcgatgcg
    23221 gaactgctct gccccgctta catcgacacc catgtacacg gtggtgcggg cgttgatgta
    23281 atggatgacg cgccggatgt actcgacaag ctggcaatgc acaaggcacg cgaaggtgtc
    23341 ggcagttggt taccgaccac cgtaaccgcg ccgcttaata ccattcatgc ggcgctgaaa
    23401 cgtattgctc aacgttgcca acgcggcgga cctggtgcgc aagtgctggg gagttatctc
    23461 gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc c
//
LOCUS       DAJVQH010000055        23392 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJVQH010000055 DAJVQH010000000
VERSION     DAJVQH010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.055"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..195)
                     /locus_tag="OQM87_002214"
     CDS             complement(<1..195)
                     /locus_tag="OQM87_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508340.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPD"
     gene            413..1273
                     /gene="tesB"
                     /locus_tag="OQM87_002215"
     CDS             413..1273
                     /gene="tesB"
                     /locus_tag="OQM87_002215"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCS1508341.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1322..2608)
                     /gene="amtB"
                     /locus_tag="OQM87_002216"
     CDS             complement(1322..2608)
                     /gene="amtB"
                     /locus_tag="OQM87_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCS1508342.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(2638..2976)
                     /gene="glnK"
                     /locus_tag="OQM87_002217"
     CDS             complement(2638..2976)
                     /gene="glnK"
                     /locus_tag="OQM87_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCS1508343.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3157..4938)
                     /locus_tag="OQM87_002218"
     CDS             complement(3157..4938)
                     /locus_tag="OQM87_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1508344.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(4931..6703)
                     /locus_tag="OQM87_002219"
     CDS             complement(4931..6703)
                     /locus_tag="OQM87_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1508345.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(6733..7191)
                     /gene="decR"
                     /locus_tag="OQM87_002220"
                     /pseudo
     CDS             complement(6733..7191)
                     /gene="decR"
                     /locus_tag="OQM87_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            complement(7345..8163)
                     /gene="cof"
                     /locus_tag="OQM87_002221"
     CDS             complement(7345..8163)
                     /gene="cof"
                     /locus_tag="OQM87_002221"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCS1508346.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8263..9962
                     /locus_tag="OQM87_002222"
                     /pseudo
     CDS             8263..9962
                     /locus_tag="OQM87_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10027..10722
                     /gene="queC"
                     /locus_tag="OQM87_002223"
     CDS             10027..10722
                     /gene="queC"
                     /locus_tag="OQM87_002223"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCS1508347.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(10774..11172)
                     /gene="fadM"
                     /locus_tag="OQM87_002224"
     CDS             complement(10774..11172)
                     /gene="fadM"
                     /locus_tag="OQM87_002224"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCS1508348.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11266..11637)
                     /locus_tag="OQM87_002225"
     CDS             complement(11266..11637)
                     /locus_tag="OQM87_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCS1508349.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNLLVERNLAVLTL"
     gene            complement(11788..13659)
                     /gene="ppiD"
                     /locus_tag="OQM87_002226"
     CDS             complement(11788..13659)
                     /gene="ppiD"
                     /locus_tag="OQM87_002226"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1508350.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(13851..14123)
                     /gene="hupB"
                     /locus_tag="OQM87_002227"
     CDS             complement(13851..14123)
                     /gene="hupB"
                     /locus_tag="OQM87_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCS1508351.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14332..16686)
                     /gene="lon"
                     /locus_tag="OQM87_002228"
     CDS             complement(14332..16686)
                     /gene="lon"
                     /locus_tag="OQM87_002228"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCS1508352.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(16874..18148)
                     /gene="clpX"
                     /locus_tag="OQM87_002229"
     CDS             complement(16874..18148)
                     /gene="clpX"
                     /locus_tag="OQM87_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCS1508353.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18274..18897)
                     /gene="clpP"
                     /locus_tag="OQM87_002230"
     CDS             complement(18274..18897)
                     /gene="clpP"
                     /locus_tag="OQM87_002230"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCS1508354.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19144..20442)
                     /gene="tig"
                     /locus_tag="OQM87_002231"
     CDS             complement(19144..20442)
                     /gene="tig"
                     /locus_tag="OQM87_002231"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCS1508355.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(20786..21103)
                     /gene="bolA"
                     /locus_tag="OQM87_002232"
     CDS             complement(20786..21103)
                     /gene="bolA"
                     /locus_tag="OQM87_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCS1508356.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21408..21986
                     /locus_tag="OQM87_002233"
     CDS             21408..21986
                     /locus_tag="OQM87_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1508357.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22030..>23392
                     /gene="ampG"
                     /locus_tag="OQM87_002234"
     CDS             22030..>23392
                     /gene="ampG"
                     /locus_tag="OQM87_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCS1508358.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEHTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLT"
BASE COUNT         5490 a         6475 c         5649 g         5778 t
ORIGIN      
        1 atcaggcggc agtgcgactt tctgacggat ccagacggta ccggagacat tcggttgctg
       61 gatagcaggt tgttgtgttg ctgaaataga cgtatttgca gccggggctg gcgtctgaat
      121 atccgcgctt ttatctgcgc aagccgccaa cgcaatcgca accgctaaac cactggccat
      181 gtgcacgagt ttcattcatt tctccttatt atcaatgcac cagcgggcta actttcctcg
      241 ccggaagagt ggttaacaaa atagtaacgt caacaagtgt ggcacacatc acgcatttct
      301 gcctgtaatt agcccgtaat tcagactatt gcacccatcg gaccagtggc aaatttgcgt
      361 tatactcaac tcactttggc ttgctgcggc agctttgtta ctggagagtt atatgagtca
      421 ggcgctaaaa aatttactga cattgttaaa tctggaaaaa attgaggaag gactctttcg
      481 cggccagagt gaagatttag gtttacgcca ggtgtttggc ggccaggtcg tgggtcaggc
      541 cttgtatgct gcaaaagaga ccgtcccgga agagcggctg gtacattcgt ttcacagcta
      601 ctttcttcgc ccaggcgata gtaagaaacc gattatttat gatgtcgaaa cgctgcgtga
      661 cggtaacagc ttcagcgccc gccgggttgc tgctattcaa aacggcaaac cgatttttta
      721 tatgacggcc tctttccagg caccagaagc gggtttcgaa catcaaaaaa caatgccgtc
      781 cgcgccagcg cctgatggcc tgccttcgga aacgcaaatc gcccaatcgc tggcgcacct
      841 gctgccgcca gtgcttaaag ataaattcat ctgcgatcgt ccgctggaag tccgtccggt
      901 ggagtttcat aacccactga aaggtcacgt cgcagaacca catcgtcagg tgtggatccg
      961 cgcaaatggt agcgtgccag atgacctgcg cgttcatcag tatctgctcg gttacgcttc
     1021 tgatcttaac ttcctgccgg tagctctaca gccgcactgc atcggttttc tcgaaccggg
     1081 gattcagatt gccaccattg accattccat gtggttccat cgcccgttta atttgaatga
     1141 atggctgctg tatagcgtgg agagcacctc ggcgtccagc gcacgcggct ttgtgcgcgg
     1201 tgagttttat acccaagacg gcgtgctggt tgcctcgacc gttcaggaag gggtaatgcg
     1261 taatcacaat taaaaaaata gccggaggtg aaaaccgtcc ggctattttt tgcagtgctt
     1321 gttacgcgtt ataggcattc tcgccgtggc tgttgacgtc cagcccttct cgctcttgtt
     1381 cttccggcac acgtaggcca accgtcagat ccgccagttt gtagccaata aatgccacca
     1441 caccggacca gacgatcgta atggcgatgc tttccaactg taccagcaac tgatggccca
     1501 tcgtcacacc ttcagcgaag cccacgccgc ccagagaact ggcggcaaaa atcccggtca
     1561 tgatacagcc gacaatgcca caaacgccgt gcacaccgaa gacatcgcag ggatcatcca
     1621 cccgcagcaa gcgtttgagc atggtaacgc cccacaaacc cgccagacca gctaccacgc
     1681 cgataatcaa cgcgccgcca accccaatgt agccgcacgc tggcgtcacg ccgaccagac
     1741 cggcaatcgc gccagaacac gctcccagca gagaaggctt accacgcagc gcccattcac
     1801 cgaagatcca gccaagaatt gccgccgccg ttgcgaccac agtattcaca aatgccagtg
     1861 ccgcgatttc attcgccgtg cccgctgacc cggcgttaaa gccaaaccaa ccgatataga
     1921 gaatggcagt cccggtgaag accatcggca ggttgtgcgg tttaaacgcc tctttaccga
     1981 agcccacgcg tttgcctatc agatacgcgc ccaccagacc ggcgattgct gcgttaatgt
     2041 gcaccaccgt gccacccgcg aaatccagcg caccgtgaga agccagcaaa ccaccgcccc
     2101 acaccatatg cgcaatcgga atgtaagaga gcgtcagcca taccaccacg aaaatcaaca
     2161 cagctgagaa gcggattcgt tccgccagcg ccccaactat caagcctacg gtaatgcagg
     2221 caaacgatcc ctgaaacgct acgtggatat actgataaat gctgcccatc accgccgtca
     2281 gttcgatgtt ttttagcatc aaccagttaa tgttgccgaa gaagttgttg ccctcaccaa
     2341 acgccagcga ataaccgtaa accacccaga gaatacagac cagtgcaaac gtcaccgtca
     2401 cctgcgtcag catcgacagc acgtttttgc cgcgaatcaa cccaccgtaa aacagggcaa
     2461 tccccggaat agtcataaac agcaccagcg cagtacaaat catcataaac gcattgtcgg
     2521 ctttatcggc caccgcaggt gcagccatta ccagtcccgg cagcatcgcc agtgaagcaa
     2581 gcccagtttt tatcgtcgct atcttcattt ttcgttcctg ttgctgtgtg ccagagatta
     2641 cagcgccgct tcgtcggctt cgccggtacg aatacgaatg acgcgttgca attcagcgac
     2701 gaagattttg ccgtcgccaa tttttccggt gtaagccgcc ttactgacga tatcgatcac
     2761 ttcatcgagt tggtcatcag caatcgccac atcaattttt acttttggca ggaagttgac
     2821 gctgtattcc gccccccggt acagctcggc atgccctttc tgacgcccga aacctttcac
     2881 ttcggtgacg gtcaggccct gaataccaat ggaagataac gcttcacgaa cgtcttccag
     2941 cttgaatggt ttgattatca cggtcaccag cttcatagat cccctccggt cagaattcgg
     3001 taatggttct gctacacgaa gaaggtattg caagcggtgt gccagaaatg aaaaacgaga
     3061 aagagagcag gaagttaaga agattgaaaa tgaaaacgca ctatgacagt gcacagtgcg
     3121 ttacattttt gcaccaacga tgaaatattg cgctattcag gcgctcaatg attcctcttc
     3181 acgcacgctg gctgccagct cttcgcccgc aagttgcagt tgatacatct gccagtagcg
     3241 tccctgggcc gccagcagtt gctggtgagt gccctgctcc acggcttgcc cacgatgaag
     3301 caccagaatg gtgtcggcat caataattgt tgataagcgg tgagcaatca ccaccagcgt
     3361 ggtatgttca cgcaccgccg ccagagcatg ttgaatcgcc tgttcagtac cggagtcaat
     3421 gctggcggtt gcctcatcaa ggatcaggat ttgcggcgtc tccaccagca cgcgcgccag
     3481 tgccagcagt tgcttttgcc cgactgagag attatttccc tgctcgccca gcggcgtata
     3541 aatacaatca ctcatgctgc gcgccagctc agccagttgc acggtttcca gcgcctgcca
     3601 gacgcgttct tcggagatat cccgccccag cgtcacgttg gcgaggaagg tatccgccag
     3661 caccaccgga tcttgctgca ccattgccac gccctggcgc agcgcgctat gacttagcga
     3721 acttaatgga cgaccatcaa ggtgaatctc accttccgtt agcgggtaat agcccatcaa
     3781 taaactggca agggtacttt taccactgcc ggtatgcccg accagcgcca caaagttacg
     3841 cgaaggcaca gagagattaa tgttctttag caccagattg tcatcgcgat aagcaaatga
     3901 cacgttatcg acttcgatgg tgccactctg taacgggtaa tcatcattgc catattgctg
     3961 gcgcggtccg tccatcagtt caaacacgcg ctcaccagca acaacagcct gttgcagcat
     4021 cgcctgttgc gtggtcagtt cgattaaggg ttcgttaagt cgcccaagat agctgataaa
     4081 cgcatacaac acgcccactt caatggtgcc gctggcggag aagccaaaca gcatcaataa
     4141 gccacaaaga atgagcgatg aaaacagact cagcagcgga cgcagcagaa aaccgtcgag
     4201 gcgcagggtt tgcatcctag ccatatagtg tgaacgactg gcctccccca tacgttcgcc
     4261 aaatcgcgcc tgctgacgaa actgctggat aacgctcatg ccattgatga tttcgttaaa
     4321 gccgtcgttg atatccgcca gataggcgcg cacacggcgg acaatcggcg tgttgtaacg
     4381 ctggtatatc accattacca ccagcaccac cgggaaaatc attatcgcca ccagcgccat
     4441 tcgccagtcg aggctgaaca tcgccaccag catcgcgccc accagtgcgg cactgcgcag
     4501 gacagttgcc actacggtaa cgtagagatc gcggatcact tcagtgtcat tagtgacgcg
     4561 ggagatcacc tgcccgacgg gttgggtatc aaactcgctt aatggctggc gtaacgcagc
     4621 atccatcacg tcggtacgca actgttgcac tacgcctact gccgcccgat taaacagcag
     4681 cgactgagcg taatgtagcc cggcggcaaa cagttgtagc ccaacatacg ccgcagccag
     4741 ccctgcaacc actttcaacg gcaggttatt tttcgctacc atattgtcga taaaatagct
     4801 gataagcagc ggcccactga cttctgccgc cgccgcaacc cacatcatca ggaccgcaat
     4861 ccccagcggt ttacgccacg gcgaaccata cgctaacagg cgcttgagag tcggccacag
     4921 ttggctaaaa ctacgcatcg acggcctcct cgcgaatttc cggaacgtcg tcgagcgccg
     4981 cctccagttg ttgatagcga tacatatcgc gataccagcc gctttgttgc gccagctcat
     5041 catgattgcc acgctgggcg atatgtccgt gctgcatcac aataatttca ctggcttccg
     5101 tcagtgccga aaggcgatgg gcactgatga ttaccgttct tccctgtccc cactgacgca
     5161 ggttatgcag gatctggtgc tctgtgcgtc cgtccaccgc cgaaagcgca tcatcaagga
     5221 tgaggatttc cgcgttgact aataacgctc gagcaatgga gatacgctgt ttttgcccac
     5281 cggaaagcat cacgccgcgc tcgcccactt ctgtatcgta accttgcggt agacgcaaaa
     5341 tatcgtcatg tacgctggct aaccgcgcga catgctcaat ctcttgctgg gtggcattcg
     5401 ggcaacccag cgcgatgtta ttcgccacgg tgtcagaaaa aaggaatggc gtctggctga
     5461 ccaccgccag gcggctacgc cagctatcga gttgtaactt cgtcagagga atatcatgaa
     5521 agcgaatatc cccctcgctg acgtcgaaat gacgctgaat gagcgacaac agggtacttt
     5581 tgccggaacc agtcggcccg caaataccca gcatctgacc gggtttcagg gcgaaattga
     5641 cgttttccag cgcaggatgg tcagtctgcg gatacgtgaa ctggtgaata tttacatcca
     5701 gttcgccacg cccttccggc acgagttcgc taccatcgtt caccaccggc gcttccgcca
     5761 gcatcgcgcg aatacggctg tatgcagcac taccacgttc cacaatgtta aacatccatg
     5821 ccagcgccag cattggccaa atcatcagac ctaaatacat cataaaactg gtgagctggc
     5881 ccagcgttaa actgccctgc accaccatcc aactaccgcc gccaatcgcc agcaagttcg
     5941 ccataccaat cgcgatatag atggtcgggt cgaagcgagc atcaatacgc gccacccgca
     6001 tgtttttttt gccggtatct tcggcatccg cggcaaataa cgccgactgg cgatcttcca
     6061 gaccaaaggc tttgatcatg cggatactgg tgaggctttc ctgggtgcgg tcattaagac
     6121 tggaaaacgc cgcctgtgcc agcttaaagc gttcatgcaa ggcatcgccg ttgcgcttaa
     6181 tcatgatcgc catcactggc atcggtaaca gggcaaataa ggtcaactgc cagctaattt
     6241 gcgtagacat cataatcaac acagcgcagc ccattaccag tgaatccacc agcgtcagca
     6301 ccccttctcc ggcggcaaac acgacgcgat cgacgtcatt ggtcgcacga gccatgagat
     6361 cacccgtgcg atgacgcagg taaaactccg ggtgctgccg gcttagctga cggtaataat
     6421 cttcacgcag ttcaacagcc agttggtaag acgcgccaaa cagcaatacg cgccagacat
     6481 aacgcaggag ataaaccaca acggcaatca gcaccatggt ggcgatccac atcaggatca
     6541 gcccggtagt aaagtgttgt tctgtcacgc catcgacaac aataccaacc acttttggcg
     6601 gaaccagttg cagcatcgcg ataatgacaa gcaaggcgac agccccaaga tagcgacgcc
     6661 attcccgacg gaaataccag cttaattgag caaataatcg cacgcgttgt gtcctgacct
     6721 gattctggat atttattcga tgggtaaaga agtggtgtat ttaatctgtt ccatcgcgaa
     6781 gctggaagtg acgtccgaca gccccggcac gctgtttacc agacgcttat aaaactcgtc
     6841 gtagcgtttc atgtcggcaa cctagacgcg catcagataa tcgtattcac cagccatgcg
     6901 ccagaacccc agcacttctg gcatttcggt aaccaccgtg acaaagcggc aataccattc
     6961 gctgctgtga tgttgcgttt ttatcagcac aaaggcggtc aggccgaggc ctattttttc
     7021 cggatccagc agggcgactt tgccgataag gataccgtcg tcctccagcc gtttcaggcg
     7081 cttccagcag ggggtggttg tcagattaac ggcttcagcc agtgcctgca aagagagggt
     7141 gcaatcctgc tgcagtaagg ccagcagctt acggtcaatt ttatctaaca tagcccttcc
     7201 acggagaatt tttttctctt ttaattctat tttaaaggtg aaatcgcgac aatttattct
     7261 gtgcggttta aacaccacgg cacaaaatga caaattcaac gcaaaaaaaa accgggcaat
     7321 tgcccggtgc tggaagggat ctcgttattc gggggaataa ggtagatgtg gatagtccag
     7381 ccagtgcgtc aaatagtgag agacagcctg atttcggcaa tgtccaatca ccggtaaatg
     7441 cgggagctcc gcgcgcagtt gcggcatcgc attgcccata ataaacccgc taccgacgct
     7501 gcctaacatt tcgcgatcgt tcatcgcatc accaaaggcc atgcaatcgc gcaacgataa
     7561 acctaaatgt tgggtcagca ccgtcaatgc agcgccttta ttgcagccca ccggcagcac
     7621 ttcgaggcaa tccgtggcgg aaaaacacaa atgtgcacgc tcgcctaatg cttcgtatag
     7681 ctggatctgc aagcgtgtaa gatcgtcgtg atcgccacag aagcagatct tggtgacgct
     7741 gccgagtggc atttttttga catcgattat ctgataacga aaaccgctat agacaaatac
     7801 ctgcaacaac gcagggctct ctttcccggt aaaccaaccg tcgtcattga agatatgcat
     7861 gctggctcgg gtatcccatt gctgatacag caccagctcc gcgacatccg caggtaaatc
     7921 atcacgatgt aaaagttcac cttccagaga atgcacgcgc gttccgttgc cggtaatcaa
     7981 atatgcatcc agcgatagcg ccccgagaat atgctgcatc tccagcgcat gacgccccgt
     8041 ggcaaaagtg agggtaatgt cgcgttcacg cagtcgcgcc aaagtagaga gggttttctc
     8101 acctaaatga tggtcaggca tcaataaagt gccatccata tcaaatgctg ccagacgagc
     8161 catttctttc tccactctgt gacacggtta atggtgattg agtatcacct gatatatacg
     8221 gaagtaatag tgaatagtta aataatattg ttccgggttt atatgcgatt gctcaaccgt
     8281 cttaaccagt atcaacgtct gtggcaacct tcagccggaa agccgcaaac cgtcaccgtc
     8341 agcgaactgg ccgaacgctg tttttgcagc gaacgccatg ttcgtacgct gttgcgtcag
     8401 gcacaggagg cgggatggct ggagtggcag gcgcagtcag gacgcggaaa gcgcggacaa
     8461 ttacgctttc tggtcacgcc ggaatcgcta cgcaatgcga tgatggaaca ggcactggaa
     8521 accggaaagc agcaagatgt gctggagctg gcgcaactgg ccccaggtga gctgcgcact
     8581 ctgttacagc cgtttatggg cggacaatgg caaacgatac acccacgttg cgtattccct
     8641 actatcgccc gctcgaaccg ctacaaccag gctttttgcc cggccgtgcc gagcagcatc
     8701 tcgccgggca gatattttcc ggcctgaccc gcttcgataa taatactcag cgcccgattg
     8761 gcgatttagc gcatcactgg gaaacctcta ctgacgggtt acgctgggac ttttatcttc
     8821 gttcaaccct acactggcat aacggcgatg cagtaaaagc ctcacactta caccagcgat
     8881 tattgatgct gttacaactg ccagcactgg atcaattatt tattagcgtg aagcgtattg
     8941 aagtcaccca tccgcagtgt ctgaccttct ttttacatcg ccccgattac tggcttgcgc
     9001 accggctggc gagctattgc agccatctgg cgcatccgca attcccactg atcggcactg
     9061 gtccttttcg cttaacacaa ttcacagcgg agctggtgcg cctggagagc catgattatt
     9121 accatttgcg tcatccgctg cttaaagcgg ttgagtactg gataactccg ccgcttttcg
     9181 aaaaagattt gggaaccagt tgtcggcatc ccgtgcaaat caccatcggc aaaccggagg
     9241 agctgcaacg ggtcagccag gtcagtagtg gcatcagttt aggtttttgc tatttaacgt
     9301 tgcgcaaaag tccccgactc tccctctggc aggcgcgaaa agtgatctcc attattcatc
     9361 aatccggttt attacaaacg ttagaagtcg gagaaaacct gatcaccgcc agtcatgcat
     9421 tactgccagg ctggactatt ccgcactggc aagtaccgga tgaagtcaaa ctaccgaaaa
     9481 ccttgacgct ggtttatcac ctaccgatag aacttcatac catggcagaa cgcctacagg
     9541 cgacactggc agcggaaggc tgtgaactca caattatttt tcataacgca aaaaactggg
     9601 acgacacgac cctactggca cacgcagacc tcatgatggg cgacagatta attggcgaag
     9661 caccggaata tactctggag caatggctac gttgcgatcc actatggcca catgttttcg
     9721 acgctccagc atatgcacat ctgcaatcga cgctggacgc ggtgcaagta atgcctgatg
     9781 aggaaaaccg atttaatgcc ctgaaagcgg tttttagcca gttaatggca gacgcgacgc
     9841 tgacgccgct gttcaactat cactatcgca ttagtgcccc tcccggcgtg aacggtgtgc
     9901 gactgacacc gcgcggctgg tttgaattta ccgaagcctg gcttcccgcg ccgtcgcaat
     9961 gaatagctgg tccgggttaa tcgcaggcgt taccataacg cttttattgt tcaagcagga
    10021 ttatctatga aacgtgctgt cgttgtgttc agtggaggcc aggattccac cacctgtctg
    10081 gtgcaggcat tacaacaata tgatgaagtc cattgcgtga cgttcgatta cggtcagcga
    10141 catcgcgcag aaatcgacgt ggcacgcgaa ctggcgctga aactgggagc acgcgcgcat
    10201 aaggttcttg atgtaacgct gctcaacgag ctggcggtca gcagcctgac acgtgacagc
    10261 attccggtgc ctgattatga acctgaagcc gatggcatcc cgaatacgtt tgtcccaggg
    10321 cgtaatattt tgttcctgac gctggcggca atatatgcgt atcaggtaaa agcagaagcc
    10381 gtaattaccg gcgtctgtga aacggatttc tccggctacc cggattgccg cgatgagttt
    10441 gtgaaagcac taaaccatgc cgtcagtttg ggcatggcga aagatattcg ttttgaaacg
    10501 ccgctgatgt ggattgataa agcggaaacc tgggcgctgg cagattatta cggcaaactg
    10561 gatttagtcc gtaacgaaac gttgacctgc tataacggca ttaaaggcga cggttgcggt
    10621 cattgtgcgg catgtaattt acgcgccaac ggtttgaatc attatctggc cgataaaccg
    10681 acggtgatgg cagcgatgaa gcagaaaacc gggttgaggt aattattccg ggtgtcgccg
    10741 gatgcggctt gagcatccgg caccacaaaa cgtttactta accatctgct ccagcttttc
    10801 gcgcaattcc ccttccagag ctaatgcttt ctgcgtttta agatcaatac aaacaaacgt
    10861 aataagcgca tccgctacca cctgcccttc cggctccagt gtaatgacct ggcttaagat
    10921 gccgctttta ccgtttaatt gctgcaactg actggtaata gttaacaggt cgcttaatac
    10981 cgctgggcga cgatagttaa tattgatatt gaccacgacg aaggcgatgt tatgggccgt
    11041 catccactga aaactgtcgc tattttccaa cccatcccag cgggcttctt cgagaaattc
    11101 aaggtagcgg gcgttgttga cgtgctggta aacgtcgaga tgatatccac gaactttgat
    11161 ttgtgtttgc atagcgcaat aaccttacgt tgttgttata agtacagagg tcataactgg
    11221 tatgacctct tcagtctggc aaaaaattgc cactatgcaa attaattaca gggttaatac
    11281 cgccagatta cgttccacca gcaaattgcc catccccggc acctgcttta gatcctccac
    11341 cgttttaaac ggaccgtact cttcgcgata actgacaatc gcctgcgctt tcttcaggcc
    11401 aacgccattc atcgcgcggg ctagctcttc cgcgctggca ttattaatgc tgacccgggt
    11461 gccttcttcg tcactggctt tcgccggtac tgctgcttta ctttgtgctg caggagcttc
    11521 cgctttggtt tctaccgccg tcggtttcgc cacagaagct gccgccagcg cgctatgaga
    11581 cattccggca caggccaggg acagggtaat gagcagtgct ttaattccgt gtttcatact
    11641 gttttctcct tgtttgttaa cagtggaatc accttaacgg gagtgaaaac agtaaacaaa
    11701 tggcaatgtt cagaaatgga aaaggccgcg aaagcgacct tttcttgtta cagctgcaac
    11761 gcattttttt gcgaggcggc ttcaggatta ttgctgttcc agcgcatcgc caattttgat
    11821 tttcgcctct ttacgcaggt tactcatcag agcttcaaag acgatttgtg cgttgttctg
    11881 ggtgataccc tgcaccatcg cttttttctg atcttccggc attgaacctt gtttcacttc
    11941 atccagcgcc agcagaacca cattaccttg catatcagtc gccataccgt agctcggttt
    12001 gtctttcgct ggcagtggca gtgcaaacgc cgcctggcta atcgggtcac gaccggaacg
    12061 gcttaaggtt ttcggctcgc caaatttcag accggcagcc tgcatagctt ccgcaccttt
    12121 gccggctttc aaatcaacca gcagtttctc agcatccact ttcgcctgtt gttcagcttt
    12181 gttgtgctga accagtgcct taacttgttc ctgaacatct gccaacggtt tcaccgcttc
    12241 cggtttgtgc tcgctgacgc gcagcacgaa tgcgcggtcg ccgtctacgg tgatgatgtc
    12301 agagttgatg cccgacgcgc cgttttcacc taccagaccg ccgttaaaga tagcgtcggc
    12361 aaccggcttg aagttcaact cttccggcag gttatctttg ctgaaccaac ccgtctgagt
    12421 ggctttaacg ccggcagctt gctctgcacc ggccagagac tcggtttcgt tgcttgccgc
    12481 atcgctcact ttctgctgca gcgcatagta cgcatcgagg gctttttcgt gtttcacttt
    12541 cgccgcaatg tcgtcacgta cttcgtctaa cgatttcact ttcgctggct gaatgtcgtc
    12601 cagacgtaca atcaggaaac cgaccgaaga tttgatgaca ccagacagtt ggcctttttc
    12661 tttcagacca gcatttttca gttcgtcggg gatagtggca tcttctaacc aacccatatc
    12721 gccgccgtta cgggcagaga taatatcggc agatttttct ttggctaatg cagcaaaatc
    12781 accgccttta ttcagctcat caagtaccgc tttcgcttca tcttcagttt tggtctggat
    12841 gatgctgtag cgggtacgct gcggctgggt gaattgatcc tgatgctgat cgtagtagct
    12901 ctggatatcc gcatcgctaa ccggttgctg catcgttgcg gcaaccagct tgatgtaact
    12961 cacgcggaat tgttccggcg tcatgaaatt gtttttgttt tgttcgtagt agctggcaat
    13021 ctcctgttcg gtcacaggct gcttcgccgc cagcgcgtta acatcgatag tcgcctcacg
    13081 caccacgcgt tgttgcgcga ccagtgccgc cagctcgtcg gtttcacctt tcagcataaa
    13141 atcggtaccg gcaacgccgt taatcagctg ttgggtagtg agctggttac gcagcgcctg
    13201 ggcgtactga tcggcggtca tccccatctg gttgaggata ccgttatagc ggctgttatc
    13261 aaatttgccg tcaacctgga aggctggggt cgcgaaaatc gcctgtttaa cctgctcatc
    13321 actgataccc agtttcagct cacgagcgta ctgatccaac agcgcctcgt cgatcagacg
    13381 attcagcacc tgttgacgca gggttttcat atagccttcg ttcgctgcca gctcggagta
    13441 ttgatcgccc agctgttgct gcatgcgatt acgctcgctg ttaaaggcgt tctcgaactg
    13501 cccacggctg atttcctggt cattcacttt tgcggcgtag ttattgcctc cgccaatcag
    13561 gtaaccactc acgccggtca atatgaacga cacgataatg ataccgaaaa taatcttgag
    13621 cacgagactg tttgcagccg tgcgtaagct gtccatcatg gtgtaacaac actccgctgt
    13681 agatgactgt gtacctcacg caacgtatcg atgcgcgcaa aggcctattg tgacaagaaa
    13741 cgggggcaat tgtcagccca caagacacag aaagtcaccg aatacaaata aaaaaggcac
    13801 atcagtagat gcgcccttga acttcgtcac atccccactg gggacaacgc ttagtttacc
    13861 gcgtctttca gtgctttacc tgcacggaag ctcggtactt tagcggcagc gatggtgatc
    13921 tctttaccgg tctgcgggtt gcggccagta cgggcagcac gctctttaac ggcaaaagta
    13981 ccaaaaccta ccagtgctac atcatcccct tctttcagag attcagttac ggaagcaata
    14041 atagcatcta acgcacggcc agccgcagct ttagagatat cagcccctgc agcaatcttg
    14101 tcgatcaatt gagatttatt cactcttctc ttcctcttta taatttatat cgcacttgaa
    14161 tccttcaagg tacgaacgcg cagcagttat atcaggccag ccatcccctt acaacacccc
    14221 ttaataaggg caagcccgat ccgctatcta acttagcgag acaaaaaaag gctggcaagc
    14281 ccgaattagc ctgccagccc tgtttttatt agtgcatttt gcgcgaggtc actattttgc
    14341 agtcacaacc tgcatgccag acggttcatt ttgcagcgcc agagtcagaa cttcctcaat
    14401 gcgcttcaca ggatgaatgt ccagatcggc aattacgttg tcaggaatct cttccagatc
    14461 gcgtttattt tcgaacggaa ttagcactgt tttaatcccg ccgcgatgcg ctgccaggag
    14521 tttttctttc aaaccaccga tcggcagtac ctgaccacgc agagtgatct caccggtcat
    14581 tgccacatcg gcacgaaccg ggttaccggt caggcaagaa accagcgcgg tgcacatagc
    14641 aataccggca ctcggaccat ctttcggcgt cgcaccttcc ggtacgtgga cgtggatgtc
    14701 acgtttttcg taaaaatcag ggttgatccc cagtttttcc gcacgcgcac gaaccaccgt
    14761 taacgccgcc tgaatagact cctgcatcac ttcgccgagc gaaccggtat aggtcagttt
    14821 gcctttaccc ggaacacatg cggtttcaat ggtcagcaag tcaccgccca cttccgtcca
    14881 cgccagaccg gttacctgac cgacacggtt ttcgttatca gcgcgaccat agtcgaaacg
    14941 ctgaacaccg aggtagtcat gcaggttatc gccgttaatt tcgatatgtt ttaatgactt
    15001 atcgagcagt aactgcttaa ccgctttgcg gcacagtttg gagatttcac gctccagacc
    15061 acgcacgccc gcctcacggg tgtagtaacg aataatgccg ataatggcgc tatcgtcgac
    15121 ggtcagctca ccttttttca gtgcattacg ttcaatctgc ttcggcagca ggtgacgttt
    15181 ggcgatgttc agtttttcat cttcggtata accggagagg cgaatcactt ccatacgatc
    15241 cagcagcggt gccggaatgt tcatggagtt cgacgtcgcg acaaacatca cgtcgctgag
    15301 atcgtaatcc acttccaggt agtggtcgct gaacgctacg ttctgctctg gatccagcac
    15361 ttcaagcagt gcagaggccg gatcgcctcg catgtcagaa gacattttgt cgatctcatc
    15421 gagcaggaac agcgggtttt tcacgcccac tttcgccatt ttctggatca gtttacccgg
    15481 catagaaccg atgtaagtac ggcggtgacc acggatttcc gcttcatcac gcacgccgcc
    15541 cagcgccata cggacatatt tacgcccggt ggctttggca atggactgac caagagaggt
    15601 tttacctacc cccggcggcc ctaccaggca gaggatcggt cccttgattt tgttgacacg
    15661 gctttgaacc gcaaggtact caaggatgcg atctttcacg cgctccagac cataatggtc
    15721 ggtatcaagg atttcctgcg cctgacgcag gtcttttttg accttgctgc gcgcattcca
    15781 cggtacctgt accatccagt cgatataacc acgcactacg gtcgcttctg ccgacatcgg
    15841 agacatcatt ttcagcttct gcaactctgc ttccgctttc tcttttgcct ctttcggcat
    15901 cttcgccgcg tcgattttgc gcttcagggc ttcgttttcg tccggcgcgt cgtccatttc
    15961 accgagttct ttctgaatag ctttcatttg ctcgttcaga tagtactcac gctgggattt
    16021 ctccatctgc tttttaacgc ggttgcgaat gcgtttctca acctgcagca gatcgatttc
    16081 cgattccatc attgccatca gatattccag acgttcgtta acgtcggaca tctccagaac
    16141 ggactgtttg tcagccagtt tcagcggcat atgtgcagca atggtatccg ccagacgcgc
    16201 cggatcgtcg atgctattca gcgacgtcag cacttctggt gggatttttt tgttcagctt
    16261 gatgtagcct tcgaactggc tgattgcagt acgcaccagc acttcctgtt cccgctcatc
    16321 aatggtcggc gactccagat actccgcctt cgcagaaaag tgttcgccat tgtcagagag
    16381 cgcagaaata cgcgcgcgct gtaacccctc gaccagcact ttgacggtgc cgtcaggcag
    16441 tttcagcatc tgcaatatag aggccacggt cccgacggtg aaaagatcgt ttacacccgg
    16501 ctcatccgtt gaagcttctt tctgcgcgac cagcataatt tttttatcat ggtccatcgc
    16561 cgcttccaga caacggatag atttttcccg cccgacaaat aaggggatga ccatgtgcgg
    16621 ataaaccacc acatcgcgca gcggcaatac ggggatttca atgcgttcag aacgctcagg
    16681 attcatagag ctctctctta gtttaatttc cgccaggtaa tcagatgaca cgactgtgct
    16741 tcacgccatc tattaacatg tacgtcagta tatggggatg tttcccccac attcaacgcc
    16801 gagaatagag gaaaaattaa aggggagata aaatcccccc tttttgttta actaattgta
    16861 tgggaatggt taattattca ccagatgcct gttgcgcttc cggcttacca taaatcagca
    16921 acggtttgct ttgaccatca attaccgact cgtcaataac cactttttcg acgtcttcca
    16981 tggacggcag atcgtacatg gtatcgagca gtgcggcttc tacgatggaa cgcaggccac
    17041 gggcaccggt tttacgcgcc atcgctttct tagcgatagc atccagcgcc tcgtcacgga
    17101 attccagatc cacgccttcc agattaaaca gcgcctgata ctgcttggtc agggcgtttt
    17161 tcggctcttt gaggatctga atcagagctt cttcgctcag ttcattcaac gttgctacaa
    17221 ccggcagacg accaatgaac tcagggataa gaccaaactt gatcagatct tccggttcaa
    17281 cctgcgccag cagctcgcct tcgcttgctt tgtcggactt cgcttttacc gtcgcgccaa
    17341 aaccaatgcc ggagccggtt tctacacggt gggaaatcac tttatccaga ccggcaaacg
    17401 caccgccaca aataaacagg atcttagagg tatcaacctg caagaattcc tgctgcggat
    17461 gtttacgccc accttgcggt ggaacagcag ctaccgtacc ttcgatcagt ttcaacagtg
    17521 cctgctgtac tccttcaccg gaaacgtctc gggtaatgga cgggttgtct gacttacgag
    17581 aaatcttgtc gatttcatcg atgtagacaa tcccgcgctg cgctttctgt acgtcgtaat
    17641 cgcacttttg caacagcttc tgaatgatgt tttcaacgtc ttcgcccaca taaccggctt
    17701 cggtcagcgt ggttgcgtcg gccatggtga acgggacgtc cagcaggcgc gccagcgttt
    17761 cagccagcag cgttttaccg gaaccggtcg gaccgatcag cagaatgtta cttttgccca
    17821 actcgacacc attgctggta tcgccgttgc gcagacgttt gtaatggttg tataccgcga
    17881 ccgccagcac ttttttcgcc tgttcctggc cgataacgta atcgtccagg tggttgcgaa
    17941 tttcatgcgg cgtcggtagc gcactgcgtt cacgatgcgg tgcaacttct ttaatctctt
    18001 cgcgaatgat gtcgttacat aaatcaacac attcgtcgca gatatacacg gatggaccgg
    18061 caatcagctt gcgcacttca tgctggcttt tgccgcaaaa agagcaatac agcaatttgc
    18121 ctgagccatc tttgcgttta tctgtcatgg gtcaaaacct cttctttgtt ctttgtgccg
    18181 cacacgacga cgcaaatgcc attctcaggc gcaagccgct tacagcgttg tgccgcccta
    18241 gataagtata gcggcacagt tgcgcctctg gcatcaatta cgatgggtca gaatcgaatc
    18301 gaccagaccg tattccaccg cttcaggggc ggaaaggaag cgatcgcgct cggtatcacg
    18361 ttcaatctgt tctaatgatt gacccgtatg aagcgccata agttcattca tgcgcccttt
    18421 aactttcaga atttcacgag catgaatttc gatatcggtc gcctggccct ggtagccgcc
    18481 caacggttgg tgaatcatca cgcgcgaatt cggcaggcaa aaacgtttac cttttgcccc
    18541 tgcggtcaac aagaaagcgc ccatcgaggc cgcctggccc atacagatgg tgctgacatc
    18601 aggcttgata aactgcatgg tgtcatagat agacatcccg gcagtgatca ccccgcctgg
    18661 ggagttaatg tacagataga tatctttttc tgggttttcc gcttccagga acagcatctg
    18721 cgccacaatc aggttagcca tgtggtcttc aacctggcca gtcagaaaaa tgacgcgttc
    18781 cttaagtaga cgagaataga tatcaaaaga gcgctcaccg cgtgaggtct gttcaatgac
    18841 catcggcacc agcgccatat ggggtgcaaa gttatctcgt tcgccgctgt atgacatttc
    18901 cgtctcctgg ataaaattga aaaaaacctg ctgtaccgat tgtaacctta tggacggatt
    18961 atcagctagt ccctgtgtta tgggtacggc atcaccatat ttcaagcata acaatctttt
    19021 gttgttacgc taacaccgaa agaggctttt cgcacggctc catgcaaaat tcatgacaaa
    19081 aaaaagccca ccacctggcg gtgacgggcc tttgtgcgaa tttagcgcgt tatgctgcgt
    19141 aaattacgcc tgctggttca tcagctcgtt gaaagtggtt tctttttcag tcactttcgc
    19201 tttcgccagt acagcttcaa cagcctgttc ttccagagca acattgcgca tgttgtccat
    19261 cagttctttg tttttgctgt agaactcgat aacttctttc ggatcttcgt acgcagaagc
    19321 catctcttcg atcaggcctt tcacgcgctc ttcgtcagct ttcagctcgt tggtgcggat
    19381 aacttcgccc agcagcaggc caacaactac gcggcgttta gcctgttctt cgaacagttc
    19441 gcgcggcagt tccagagctt gtttttcgtt gccaccgaaa cgctgtgcag cctggcgacg
    19501 cagaacgtcg atttcgctgt cgatcagcgc agccggtacg tcgatgtcgt tagcttttac
    19561 cagaccttcg atcgcctgag acttaacgcg gttacggatg gcgctcttca gctcgcgctc
    19621 catgttttta cgcacttcag cgcgcagacc ttctacggaa ccatcttcaa cgccgaaacg
    19681 tttgatgaat tctgcagtca gttccggcag ttcacgctct tcaactttct tcaggttgat
    19741 agcgaatttc gctgctttac ctttcaggtt ttctgcgtgg tattcttccg ggaaggtcac
    19801 gtcgatggtg aactcttcgc cagctttgtg gcctttgata ccgtcttcaa agcccgggat
    19861 catacgaccc tggcccatcg ccagtacgaa atcagacgct ttaccgcctt cgaactcttc
    19921 gccgtctaca gaaccggtga agtcgatggt tacgcggtct tctgcttcaa cagcgccgtc
    19981 tttttctttc caggtcgcct gctgtttacg cagagtatcc agcatgccgt caacgtcagc
    20041 gtcggtcact tcaacgatcg gtttttcaac ttcgatcgct tccagaccct gcagttcaac
    20101 ttccggataa acttcaaact ctacagagta agtgaagtct tcacccagct tgtattcgcc
    20161 cggaacataa gtcggtgcgc cagccggatt gattttttct ttaatgatgg cgtcaatgaa
    20221 gttacggctc atcaggtcac ccagaacgtc ctggcgtaca gacgcgccat aacgctgagc
    20281 aacgatattc attggcactt tgcctttgcg gaagccgtca atacgtactt ttttcgcaac
    20341 gttgaccagc tcgcttttaa cagcggtctc gatgctgtca gcagcgatag taatcgttac
    20401 acggcggcca aggccttgag tggtttcaac tgaaacttgc atcttgttac ctcaaaaaat
    20461 cacagtgctc ggtcaactct taccccgcaa gcacaggttg ttggcttcgc ggaaccggga
    20521 tgttcttata atcaatcaca ttccctgtta ccagaatcat cccgaaagca ttcaaatagg
    20581 acgcggcatt atagcggcat cacttttatg agtcgagaac ggttattgcg cgctgtcgcg
    20641 tattttttca caattcccgg gcaattttcg tctgaatgct gggcaaaaag cgcaaaatcc
    20701 agacaaaaca aaacggcccg caggccgttt ttcataaaat ctttagcaac atactggaaa
    20761 agcgccgaca gttgcaaatg cgtttttacg cgatgcttcc tgctccacga cagggaggag
    20821 aggcaaagac ggtgtcctgc aacccttccc actccttaat agtgtaagta tgcagagcca
    20881 gcgcatgaac ggtagtagag agttcctccg ctaaagtact gtaaatcatt cgatgacgat
    20941 tcagaaaacg ttcacccgta aaacgatcgc tgaccagcac aactttaaaa tggctttcag
    21001 agccggctgg gacattgtga cgatagcttt catccactac ttcgaggaat acgggttgga
    21061 acgccgccct taatttttct tctatccgct cacgtatcat catgaaatat ctcctccgac
    21121 aacgctgaga tgtcatccat ccctttaaat actagccgct tttaccgttt ccattgcagc
    21181 ttaacaacaa atatttaggt ttcgtcctgg tttttccgtc aagcgtatga agtttactga
    21241 tttaaagatt gaaacacagg cctcgcatca gcgtttcttt taccaggggt gattatttcg
    21301 tcaacatgat gaatttacat gccttaccca cttcccctcg ccgttggcaa tgttatgatg
    21361 gcgggaattt ttaccaaaca cgcttaagag tctctgagag cccgaacatg ttcaaaaaaa
    21421 tcctcttccc gttagttgct ctgtttatgc ttgcaggatg cgcaaaaccg ccaacaacta
    21481 ttgaagtttc cccgacgatt acactgccac agcaggatcc aagcctgatg ggcgtcaccg
    21541 taagcattaa tggtgccgat cagcgtacag atcaggcgct ggcaaaagtc acccgcgata
    21601 atcaaatcgt taccctgacc gcctcccgcg atctgcgttt cctgctgcaa gaagtgctgg
    21661 aaaaacagat gaccgcgcgt ggttacatgg ttggtccgaa tggcccggtt aatctgcaaa
    21721 tcatcgttag ccaactgtat gctgacgtgt cccagggcaa cgtgcgctac aacatcgcta
    21781 ccaaagcgga tatcgccatc atcgctaccg cgcagaatgg caacaaaatg accaaaaact
    21841 atcgtgccag ctacaacgtt gaaggtgcgt tccaggcctc caacaaaaat atcgccgatg
    21901 cggttaacag tgtgctgacc gataccatcg ctgatatgtc tcaggacacc agcattcacg
    21961 aattcatcaa gcagaacgcg cgttaatttc tgccctctgg cccggtgcaa gccgggcctg
    22021 tagacgccca tgtccagtca atatttacgt atttttcaac agccgcgttc agccatattg
    22081 ctgatcctgg gttttgcttc cgggctaccg ctcgccctga catccggcac cttacaggcc
    22141 tggatgacgg tcgaaaatat cgatctcaaa accattggtt tcttctctct ggtaggccag
    22201 gcttacgttt ttaaattcct ctggtcaccg ctgatggacc gctacacgcc cccatttttt
    22261 gggcggcggc gcggttggct gctcgccacg caaatcctgt tattagtcgc cattgcggcg
    22321 atgggttttc tcgaaccagg cacccaactc cgctggatgg cggcgctggc ggtggtgatc
    22381 gctttttgct ctgcctccca ggatattgtc ttcgatgcgt ggaaaaccga tgtgctcccg
    22441 gcagaagaac gtggtgcggg cgcggcaatc agcgtgctgg gttaccgttt agggatgctg
    22501 gtttccggcg gcctagccct gtggctggca gataaatggt tgggctggca gggcatgtac
    22561 tggttgatgg cggcgctgtt gatcccctgt attatcgcga cgttgcttgc accagaacca
    22621 accgacacca ttcctgtgcc caaaacgctg gaacaagcgg ttgttgcacc tctgcgagat
    22681 ttctttggtc gcaataatgc ctggcttatt ttgcttctta tcgtgctgta taagctgggc
    22741 gacgcattcg ccatgagcct gacaaccacg tttttgattc gcggcgtcgg gtttgatgcg
    22801 ggtgaagtag gcgtggtaaa caaaacgctt ggcttactgg cgaccattgt tggcgcattg
    22861 tacggtggga ttttgatgca gcgcctgtcg ctgttccggg cgctgctgat tttcggcatt
    22921 ttacaaggtg cgtctaacgc tggttactgg ctgctgtcga ttactgataa gcatctctac
    22981 agcatgggcg cagccgtctt tttcgaaaac ctctgtggcg ggatgggcac atcagccttt
    23041 gtcgcgctgt taatgacgct gtgtaataag tcattttccg ctactcaatt tgccctgctc
    23101 tcagcgcttt ccgctgtagg gcgagtttat gtcggccccg tggcgggttg gtttgttgaa
    23161 gcacacggct ggtcgacatt ctatctattc tccgtcgccg ctgccgtacc ggggcttatt
    23221 ttgctgctgg tttgccgcca gacgcttgaa catacacgag taaatgacaa ctttatctcc
    23281 cgtaccgaat atccggcagg ttatgccttt gccatgtgga cactggcggc gggcgtcagc
    23341 ctgttggccg tgtggttact tctgttgacg atggacgcgc tggatttgac gc
//
LOCUS       DAJVQH010000056        23302 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJVQH010000056 DAJVQH010000000
VERSION     DAJVQH010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.056"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OQM87_002235"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OQM87_002235"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCS1508359.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQT
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OQM87_002236"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OQM87_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCS1508360.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OQM87_002237"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OQM87_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCS1508361.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OQM87_002238"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OQM87_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCS1508362.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OQM87_002239"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OQM87_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCS1508363.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OQM87_002240"
     CDS             5377..5835
                     /locus_tag="OQM87_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCS1508364.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OQM87_002241"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OQM87_002241"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCS1508365.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OQM87_002242"
     tRNA            6887..6971
                     /locus_tag="OQM87_002242"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OQM87_002243"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OQM87_002243"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OQM87_002244"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OQM87_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS1508366.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OQM87_002245"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OQM87_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS1508367.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OQM87_002246"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OQM87_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1508368.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OQM87_002247"
     CDS             complement(10252..13995)
                     /locus_tag="OQM87_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCS1508369.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OQM87_002248"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OQM87_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCS1508370.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OQM87_002249"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OQM87_002249"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCS1508371.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OQM87_002250"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OQM87_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCS1508372.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVIFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OQM87_002251"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OQM87_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCS1508373.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OQM87_002252"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OQM87_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCS1508374.1"
                     /translation="MKKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OQM87_002253"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OQM87_002253"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCS1508375.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OQM87_002254"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OQM87_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCS1508376.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OQM87_002255"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OQM87_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCS1508377.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         5841 a         6083 c         5612 g         5766 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ccccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgccgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgtttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaat gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcctcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaagcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg accggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttattggtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgctcccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc gcggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acggacatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatggccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttagctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct gtagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaaaccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggt ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgatca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgcggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtgat attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcgcatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccaaag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatga aaaaaatcgt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cagacatttt taccgctcat
    21301 tttgaaacca ctaacgggac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggcgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggttttttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaacc gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga gg
//
LOCUS       DAJVQH010000057        23012 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJVQH010000057 DAJVQH010000000
VERSION     DAJVQH010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.057"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            580..2508
                     /gene="thrS"
                     /locus_tag="OQM87_002256"
     CDS             580..2508
                     /gene="thrS"
                     /locus_tag="OQM87_002256"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCS1508378.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2512..3054
                     /gene="infC"
                     /locus_tag="OQM87_002257"
     CDS             2512..3054
                     /gene="infC"
                     /locus_tag="OQM87_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCS1508379.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3151..3348
                     /gene="rpmI"
                     /locus_tag="OQM87_002258"
     CDS             3151..3348
                     /gene="rpmI"
                     /locus_tag="OQM87_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCS1508380.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3401..3757
                     /gene="rplT"
                     /locus_tag="OQM87_002259"
     CDS             3401..3757
                     /gene="rplT"
                     /locus_tag="OQM87_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCS1508381.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3874..3998
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3880..3924
                     /gene="pheM"
                     /locus_tag="OQM87_002260"
     CDS             3880..3924
                     /gene="pheM"
                     /locus_tag="OQM87_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCS1508382.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4207..5190
                     /gene="pheS"
                     /locus_tag="OQM87_002261"
     CDS             4207..5190
                     /gene="pheS"
                     /locus_tag="OQM87_002261"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCS1508383.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5205..7592
                     /gene="pheT"
                     /locus_tag="OQM87_002262"
     CDS             5205..7592
                     /gene="pheT"
                     /locus_tag="OQM87_002262"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCS1508384.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRIAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7597..7896
                     /gene="ihfA"
                     /locus_tag="OQM87_002263"
     CDS             7597..7896
                     /gene="ihfA"
                     /locus_tag="OQM87_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCS1508385.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7997..8977
                     /gene="btuC"
                     /locus_tag="OQM87_002264"
     CDS             7997..8977
                     /gene="btuC"
                     /locus_tag="OQM87_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCS1508386.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9040..9591
                     /gene="btuE"
                     /locus_tag="OQM87_002265"
     CDS             9040..9591
                     /gene="btuE"
                     /locus_tag="OQM87_002265"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCS1508387.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9591..10340
                     /gene="btuD"
                     /locus_tag="OQM87_002266"
     CDS             9591..10340
                     /gene="btuD"
                     /locus_tag="OQM87_002266"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCS1508388.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10418..10882
                     /locus_tag="OQM87_002267"
     CDS             10418..10882
                     /locus_tag="OQM87_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1508389.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11129..11831
                     /gene="ydiV"
                     /locus_tag="OQM87_002268"
                     /pseudo
     CDS             11129..11831
                     /gene="ydiV"
                     /locus_tag="OQM87_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11895..13298
                     /locus_tag="OQM87_002269"
     CDS             11895..13298
                     /locus_tag="OQM87_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCS1508390.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPD"
     gene            complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="OQM87_002270"
     CDS             complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="OQM87_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCS1508391.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="OQM87_002271"
     CDS             complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="OQM87_002271"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCS1508392.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="OQM87_002272"
     CDS             complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="OQM87_002272"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCS1508393.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16026..18404
                     /gene="ppsA"
                     /locus_tag="OQM87_002273"
     CDS             16026..18404
                     /gene="ppsA"
                     /locus_tag="OQM87_002273"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCS1508394.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18461..20107)
                     /gene="fadK"
                     /locus_tag="OQM87_002274"
                     /pseudo
     CDS             complement(18461..20107)
                     /gene="fadK"
                     /locus_tag="OQM87_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20164..20457)
                     /locus_tag="OQM87_002275"
     CDS             complement(20164..20457)
                     /locus_tag="OQM87_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1508395.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20454..21743)
                     /locus_tag="OQM87_002276"
     CDS             complement(20454..21743)
                     /locus_tag="OQM87_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1508396.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFIYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21799..22737)
                     /locus_tag="OQM87_002277"
     CDS             complement(21799..22737)
                     /locus_tag="OQM87_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCS1508397.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIISLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTQGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22757..>23012)
                     /locus_tag="OQM87_002278"
     CDS             complement(22757..>23012)
                     /locus_tag="OQM87_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCS1508398.1"
                     /translation="PAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAP
                     LAELVGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5671 a         5615 c         6075 g         5651 t
ORIGIN      
        1 tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg actcataatc
       61 tattcgcctt atatatatct ggaggttttg aggatgatga ttaattatta tcgccagaac
      121 gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt ttatcattgt gatatgacca
      181 cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc aaatcacgca acgaactttg
      241 ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa acctttcaga cgcaccgtga
      301 tgttatcagt ggttctttaa gcgttttgct ggtgtactca ctacaaacga attgcgaatc
      361 gaatcaatgt gaaacggaaa ggtacaatct cccttttttt agtgttaaca tcgttcaacc
      421 gggtggaagg tgagaggtta atgtctttca tttgtgtacc tgattgcgaa ccaatttagc
      481 atttgttggc tagatggttt cgcaatgaac tgttaataaa caaatttttc tttgtatgtg
      541 atctttcgtg tgggtcacca ctgcaaataa ggatataaaa tgcctgttat aactcttcct
      601 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
      661 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
      721 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
      781 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
      841 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
      901 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
      961 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1021 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1081 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1141 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1201 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1261 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1321 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1381 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1441 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1501 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     1561 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     1621 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     1681 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     1741 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     1801 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     1861 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     1921 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     1981 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2041 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2101 gtaatgattc accgcgcaat tctggggtcg atggagcgtt tcatcggtat cctgaccgaa
     2161 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2221 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2281 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2341 cgtcgcgtcc catatatgct ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2401 gttcgcaccc gccgtggtaa agacctggga agtatggacg taaatgaagt gatcgagaag
     2461 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     2521 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     2581 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     2641 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     2701 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     2761 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tagtacagat
     2821 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     2881 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     2941 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tagtcgaatc cttcccaacg
     3001 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3061 caagtagcac tatctgtgga gcctttgggc ttcacaggca ttgttcgcct ttgtttcgtt
     3121 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3181 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3241 cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3301 tccaaaggcg atctgggctt ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3361 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3421 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3481 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     3541 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     3601 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     3661 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     3721 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     3781 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     3841 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     3901 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     3961 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4021 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagtg cggagaaacc
     4081 ggagcgtact taagtacgtg aggatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4141 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4201 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4261 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4321 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4381 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4441 gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc tctgccaggt
     4501 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     4561 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     4621 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     4681 tggtttgacg ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     4741 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gtgtttatcg taacgactac
     4801 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     4861 agctttacca acctgaaagg cacgctgcac gacttcctgc gtaacttctt tgaggaagat
     4921 ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg agccttctgc agaagttgac
     4981 gtcatgggta aaaacggtaa atggctggaa gtgctgggct gcgggatggt gcatccgaac
     5041 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5101 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5161 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5221 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tacgctggcg aatcaaatca
     5281 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5341 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5401 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg cggtgcgcca aactgccgtc
     5461 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     5521 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttt tctgagctgg
     5581 gaatttctga cgatcacagt ggcattatcg aactgcctgc ggatgcgccg attggcaccg
     5641 acatccgcga atacctgaaa ctcgatgata acacgatcga aatcagcgtg acaccaaacc
     5701 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     5761 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     5821 cagtcgaagc gccggaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     5881 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     5941 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6001 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatagcaaaa gagggcgaaa
     6061 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6121 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6181 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6241 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6301 cggtactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6361 gtgaggctgg cccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6421 tcactctacg tcgtagcaaa ctggatcgcc tgataggcca tcatattgct gatgagcagg
     6481 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gaatggcagg
     6541 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtc gaagaagtcg
     6601 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     6661 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     6721 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     6781 caggcgttga agccttactg ctggcaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     6841 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     6901 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     6961 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7021 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgaccttgaa tccgttctcg
     7081 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7141 cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt ggagttgttc
     7201 atcctgaact ggaacgtaaa ctagatctta acggtcgcac tctggtgttc gaactggagt
     7261 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7321 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7381 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7441 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7501 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     7561 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     7621 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     7681 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     7741 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     7801 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     7861 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     7921 atgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     7981 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8041 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8101 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8161 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8221 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8281 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8341 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8401 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8461 ctgcagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     8521 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     8581 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     8641 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     8701 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     8761 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     8821 acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggccgata ttgtagcgcg
     8881 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     8941 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9001 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9061 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9121 ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9181 aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9241 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9301 ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg ccatccgctg
     9361 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9421 gcccgtatgg tcagcaaagg ccgtgcaccg ctgtaccagg atgatatttt atggaatttt
     9481 gaaaaattcc tagttggcag ggacggaaaa gtcatccagc gtttttcccc ggatatgacg
     9541 ccggaagatc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
     9601 tgatgcagtt acaggatgtt gcggaatcta cccgcctggg gccgctttca ggcgaggttc
     9661 gggctgggga aatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
     9721 cgcgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg caaccactgg
     9781 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
     9841 cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat aaaacgcgta
     9901 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggtcgtagca
     9961 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgttgcaaa
    10021 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10081 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgcgctgtgt cagcaaggac
    10141 tggcgattgt gatgagcagt cacgatctca accacacgtt gcgtcatgcg catcgggcgt
    10201 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10261 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10321 tgctgatttc gaccatctga tttcattagt atcccgaaaa tcgggtaatt acgacgctaa
    10381 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10441 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10501 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    10561 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    10621 gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa accaaacaac aagcctctat
    10681 tggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    10741 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    10801 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    10861 ctggcaggcg agacgaatct agtgtgtcga aaaattgacg gcgatgatac atcgccgtca
    10921 atttactcct gaatacagta gtattttgca gacaaaaatt ccgtaaatgg attattcgtt
    10981 tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt ggttaacatg gaacaaatga
    11041 aagttactcc ttctgtaatt gttatgtctt atggcctcat cccttttagc cggatactga
    11101 aaaacatcct tcgagaggga cggttaccat gaagattttt ttggagaatc tttatcattc
    11161 tgattgctat ttcctgccga taagagataa ccagcaactc cttgtcggcg ttgagttaat
    11221 tacccatttc tccagtgaag atggcacggt tcgtatcccg acgagtcggg ttatagcgca
    11281 acttaccgag gagcaacatt ggcaattatt ttcagagcag ttggaattac taaaatcatg
    11341 ccaacacttt tttattcaac ataaactctt tgcctggctt aatttaacgc cacaagtgac
    11401 tacattatta ttagacaggg ataattacgc aggcgaacta ttaaaatatc cgtttattga
    11461 gctgttgatt aatgagaatt acccgcatct caatgaagga aaagataatc gcgatctttt
    11521 gtccttatcg caaatgtatc cccttgtgtt aggcaatttg ggagcaggta atagcacaat
    11581 gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg gagaaaggaa caaatcacgc
    11641 atcgctcctt cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt
    11701 gcatcatcgc ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt
    11761 tccatgctct ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg
    11821 ttcagcgata accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca
    11881 aacagatagg atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa
    11941 cctatacagc actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg
    12001 aactcgctaa cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg
    12061 gcggcgaaac cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt
    12121 tcggcgtctg ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc
    12181 ttgctgatgg cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc
    12241 gaatgggtga tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg
    12301 cgatgcatta tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc
    12361 cagtgtatcg ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc
    12421 tgcgctttgg tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt
    12481 tggctgactt tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc
    12541 gtctctggtt taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg
    12601 tcggctttgc acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg
    12661 attacgggcc gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg
    12721 atcatcaagg gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc
    12781 gtctggcgca gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca
    12841 gctatcagca ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca
    12901 tgacggagca aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag
    12961 agcgcagcga ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg
    13021 cgtcaccgct acgtgatgag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt
    13081 atcgggggcg tttgcaacaa gacgaggtta gcgatagtga gcgtcaacaa ctgatgcaaa
    13141 gcgttaaccc agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag
    13201 aaaagggtga tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg
    13261 acagagatga tgactatgtc agtcgtccac ctgactgagg taaacggctg gaagtcagct
    13321 gctcgagtta aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga
    13381 tattcttgcc cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg
    13441 gtaattcgcc ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg
    13501 agttttctgc tatccgtata acgcataaaa attaataata attgatataa caatgattcc
    13561 tattatcaat attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg
    13621 cgcagaaatg cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa
    13681 aggcgagttt ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg
    13741 attgaccgta agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac
    13801 tttccgccat aattccagca atctccgtag agccattgcg gatttgctga caaatatcct
    13861 cacaaacttc taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca
    13921 ccagatgttc aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg
    13981 catgataatt cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg
    14041 tctgatagat ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg
    14101 ctgcgcggat agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta
    14161 ccggacagga gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc
    14221 caatcgcgcc ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact
    14281 cagtcgcggt agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca
    14341 gaccgtgatt tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc
    14401 cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt
    14461 actggttgcg cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga
    14521 tcgagcaggg gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa
    14581 ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg
    14641 cgagttcggc gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt
    14701 tcattacaag tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca
    14761 tcacgataaa ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg
    14821 ttagcatgat aacaaaaaaa caaaccaata cactagttct ctagtacatt cggcgactaa
    14881 ggcccatgat atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa
    14941 tccacgggat ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact
    15001 gacgaagcga ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt
    15061 ccgggtcgat agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca
    15121 ccagattatc catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca
    15181 ttgccagata cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct
    15241 gagcctgatc cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg
    15301 ccgcaatgcg cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag
    15361 caatcggcgt tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga
    15421 tatcctggca aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg
    15481 agtagaagac cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca
    15541 ctgcccgtgc acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg
    15601 gaaattgtga cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa
    15661 tataaaaaac gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata
    15721 attctatgaa agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata
    15781 aaatgaacct tcattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat
    15841 accgatggtt tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc
    15901 ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggtaaaat
    15961 atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt
    16021 gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga
    16081 atgatgtaga cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt
    16141 ccggaatggg tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt
    16201 ttctggacca aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg
    16261 acgatgttac tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc
    16321 ccttccagcc tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg
    16381 acgaaaacgc ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt
    16441 cctttgccgg tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg
    16501 cagtgaaaca tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc
    16561 agggttacga tcaccgtggc gtggcgctct ccgccggtgt tcagcggatg gtgcgctccg
    16621 acctcgcatc ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg
    16681 tgtttatcac ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg
    16741 atgagtttta cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca
    16801 ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg
    16861 ttaaaatcga agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag
    16921 tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata
    16981 ttgagtgggc gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa
    17041 ccgtgcgctc acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta
    17101 tcgccgaagg ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg
    17161 acatcagcga aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc
    17221 cggactggga accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta
    17281 cctgtcacgc ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag
    17341 atgcaacaga acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata
    17401 ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga
    17461 tgccggatct gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact
    17521 tcgcctgcct gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta
    17581 tgattggcgt ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa
    17641 acgaaatccg cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc
    17701 tgactgaagg gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc
    17761 tctctgattt taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag
    17821 atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc
    17881 gcgactgctt cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga
    17941 ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg
    18001 aagaactggc gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt
    18061 gtgaaatccc gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct
    18121 caattggctc aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg
    18181 tgtctgaatt gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc
    18241 gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg
    18301 aagactttgc cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca
    18361 ccgtggtgca aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg
    18421 cgttcagtcg ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga
    18481 catcctgcgt taatcgccgt ataatatctt tccttaacaa aaacttttgt attttatctg
    18541 aggcagtgcg cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga
    18601 cccgtttacg gctaaaaaaa gccaccatct cttccagcga taatgaatga tgcggcgctt
    18661 tctgcacgac ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac
    18721 aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat
    18781 tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct
    18841 catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg
    18901 ttaattcagg ttcatcaaaa taccccataa acacattggg gccacgcgat gtttcttcac
    18961 cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg
    19021 cggcagcgta accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg
    19081 catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgctg cgctgctggc
    19141 attcgcgcgc cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg
    19201 aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc
    19261 ctagcataca ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa
    19321 tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc
    19381 ccgttgcgtg accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc
    19441 gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg
    19501 gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta
    19561 tcgccgtggt cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg
    19621 cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg
    19681 gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg
    19741 cctgacactt attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca
    19801 cggaaaccgc accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag
    19861 gcagttgaaa tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca
    19921 gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca
    19981 cggcaatttt gtctggcatc gcacgagcgg tctgctgtca gtaatcggcc agtgaagcat
    20041 cgccccatag cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca
    20101 ctttcatttt ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca
    20161 tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag
    20221 aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa
    20281 atgaatgttt cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt
    20341 acggaattca ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac
    20401 atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc
    20461 gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt
    20521 cccatgatca ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc
    20581 atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc
    20641 gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg
    20701 tattgcgcca gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg
    20761 gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc
    20821 aggcagaagc ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc
    20881 attgccagac cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta
    20941 atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt
    21001 tgcgcatggg cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg
    21061 gtatagataa atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg
    21121 gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata
    21181 acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag
    21241 cggccaagca ttgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca
    21301 tccccagcct gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg
    21361 cctgggataa actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga
    21421 ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gttgctcgcg gtgaaaatcg
    21481 agggtaacgg cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct
    21541 accggcgctg atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc
    21601 ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc
    21661 ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg
    21721 gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag
    21781 atgtggttga aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta
    21841 gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt
    21901 gatggcgaca atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat
    21961 cccctgcgtc aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc
    22021 acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag
    22081 caccgccgcc agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac
    22141 gccaaccaca cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc
    22201 gcgacagaga atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg
    22261 agaggtatca ctggtgcacg gttcctgaac accgggtgca agggaaatga tcgccagcgg
    22321 gctgttgatc ttttcctgag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat
    22381 gtgaccatcg acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac
    22441 acttaaccgt gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg
    22501 gtgtttatct ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc
    22561 gtcgttttgc tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg
    22621 gtcggtattt tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag
    22681 ttcagcataa cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt
    22741 ttcttctccg taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg
    22801 cctccggcga atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa
    22861 tgccgaccag ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact
    22921 gatttaccgg ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt
    22981 taatatcgga ggtgacgcag agcacggctg ga
//
LOCUS       DAJVQH010000058        22597 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJVQH010000058 DAJVQH010000000
VERSION     DAJVQH010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.058"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..610
                     /locus_tag="OQM87_002279"
     CDS             <1..610
                     /locus_tag="OQM87_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1508399.1"
                     /translation="PGLKAVGSYRSVLELLNIPHAKLIMDCVILLSVTSCLNSALYTA
                     SRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDS
                     SGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVVMLFR
                     PAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            631..1289
                     /gene="csiR"
                     /locus_tag="OQM87_002280"
                     /pseudo
     CDS             631..1289
                     /gene="csiR"
                     /locus_tag="OQM87_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(1290..1739)
                     /gene="kbp"
                     /locus_tag="OQM87_002281"
     CDS             complement(1290..1739)
                     /gene="kbp"
                     /locus_tag="OQM87_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCS1508400.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(1823..1981)
                     /locus_tag="OQM87_002282"
     CDS             complement(1823..1981)
                     /locus_tag="OQM87_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCS1508401.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2164..2463
                     /locus_tag="OQM87_002283"
     CDS             2164..2463
                     /locus_tag="OQM87_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1508402.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2473..2997
                     /gene="ygaP"
                     /locus_tag="OQM87_002284"
     CDS             2473..2997
                     /gene="ygaP"
                     /locus_tag="OQM87_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCS1508403.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(3044..3448)
                     /gene="stpA"
                     /locus_tag="OQM87_002285"
     CDS             complement(3044..3448)
                     /gene="stpA"
                     /locus_tag="OQM87_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCS1508404.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            4116..4553
                     /gene="alaE"
                     /locus_tag="OQM87_002286"
     CDS             4116..4553
                     /gene="alaE"
                     /locus_tag="OQM87_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCS1508405.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(4590..4940)
                     /locus_tag="OQM87_002287"
     CDS             complement(4590..4940)
                     /locus_tag="OQM87_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCS1508406.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
     gene            5092..5421
                     /locus_tag="OQM87_002288"
     CDS             5092..5421
                     /locus_tag="OQM87_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1508407.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     QLSMRKS"
     gene            5669..5914
                     /gene="nrdH"
                     /locus_tag="OQM87_002289"
     CDS             5669..5914
                     /gene="nrdH"
                     /locus_tag="OQM87_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCS1508408.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            5911..6321
                     /gene="nrdI"
                     /locus_tag="OQM87_002290"
     CDS             5911..6321
                     /gene="nrdI"
                     /locus_tag="OQM87_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCS1508409.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            6294..8438
                     /gene="nrdE"
                     /locus_tag="OQM87_002291"
     CDS             6294..8438
                     /gene="nrdE"
                     /locus_tag="OQM87_002291"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCS1508410.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            8448..9407
                     /gene="nrdF"
                     /locus_tag="OQM87_002292"
     CDS             8448..9407
                     /gene="nrdF"
                     /locus_tag="OQM87_002292"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCS1508411.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            9762..10964
                     /gene="proV"
                     /locus_tag="OQM87_002293"
     CDS             9762..10964
                     /gene="proV"
                     /locus_tag="OQM87_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCS1508412.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            10957..12021
                     /gene="proW"
                     /locus_tag="OQM87_002294"
     CDS             10957..12021
                     /gene="proW"
                     /locus_tag="OQM87_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCS1508413.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            12079..13071
                     /gene="proX"
                     /locus_tag="OQM87_002295"
     CDS             12079..13071
                     /gene="proX"
                     /locus_tag="OQM87_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCS1508414.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            13263..14442
                     /locus_tag="OQM87_002296"
                     /pseudo
     CDS             13263..14442
                     /locus_tag="OQM87_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            14566..15303
                     /gene="ygaZ"
                     /locus_tag="OQM87_002297"
     CDS             14566..15303
                     /gene="ygaZ"
                     /locus_tag="OQM87_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCS1508415.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            15293..15628
                     /gene="ygaH"
                     /locus_tag="OQM87_002298"
     CDS             15293..15628
                     /gene="ygaH"
                     /locus_tag="OQM87_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCS1508416.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            15719..16249
                     /gene="emrR"
                     /locus_tag="OQM87_002299"
     CDS             15719..16249
                     /gene="emrR"
                     /locus_tag="OQM87_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCS1508417.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            16376..17548
                     /gene="emrA"
                     /locus_tag="OQM87_002300"
                     /pseudo
     CDS             16376..17548
                     /gene="emrA"
                     /locus_tag="OQM87_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            17565..19103
                     /gene="emrB"
                     /locus_tag="OQM87_002301"
     CDS             17565..19103
                     /gene="emrB"
                     /locus_tag="OQM87_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCS1508418.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(19167..19682)
                     /gene="luxS"
                     /locus_tag="OQM87_002302"
     CDS             complement(19167..19682)
                     /gene="luxS"
                     /locus_tag="OQM87_002302"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCS1508419.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(19832..21388)
                     /gene="gshA"
                     /locus_tag="OQM87_002303"
     CDS             complement(19832..21388)
                     /gene="gshA"
                     /locus_tag="OQM87_002303"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1508420.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(21461..21889)
                     /locus_tag="OQM87_002304"
                     /pseudo
     CDS             complement(21461..21889)
                     /locus_tag="OQM87_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
     gene            complement(21886..22452)
                     /gene="yqaB"
                     /locus_tag="OQM87_002305"
     CDS             complement(21886..22452)
                     /gene="yqaB"
                     /locus_tag="OQM87_002305"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCS1508421.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         5436 a         5758 c         5664 g         5739 t
ORIGIN      
        1 gccggggctg aaagccgttg gatcttatcg ctcggttctg gaattgctca atattcccca
       61 tgcgaaatta atcatggact gtgtgatatt actttccgta accagttgcc tgaactcggc
      121 cctgtatacc gcctcaagga tgctctactc cttaagccgt cgcggtgatg ctcccgcggt
      181 aatgggtaaa atcaaccgca gtaaaacccc gtacgtggcg gtgttactct ccaccggcgc
      241 agcattctta acggtggtgg tgaactatta cgcgcctgcg aaggtattta aatttctgat
      301 cgacagctcc ggcgctatcg ccctgctggt ttatttagtc atcgccgttt cacagttgcg
      361 gatgcgcaaa attctgcgag cagaaggaag cgaaattcgc ttgcgaatgt ggctttatcc
      421 ctggctcacc tggctggtca tcggctttat tacctttgtg ttggtagtga tgctattccg
      481 tccggcgcaa cagttagaag tgatctccac cggcttatta gcgataggga ttatctgtac
      541 cgtgccaatt atggctcgct ggaaaaagct ggtattgtgg caaaaaacac ccgttcataa
      601 tacgcgctga taatgatcag gagtcacacc atgaccatta cgtctctgga tggctatcgc
      661 tggctgaaga acgatattat tcgcggtaat tttcaaccgg atgaaaaatt acggatgagt
      721 ttactgacat cgcgttatgc acttggcgtt gggccgttgc gggaagcact ttctcaactg
      781 gtggcggaac ggctggtcac ggtggtgaat caaaaagggt atcgggtggc gtctatgtca
      841 gagcaggagc tgctctgaca tagacgccac ccgatatgga agcgatgctg gtcagtctgg
      901 cgattgcccg cggtggcgat gaatgggagg cagacgttct cgcaaaagcg catctgctca
      961 ataagctgga agcctgtgac gccagcgaga aaatgcttga tgagtgggat ctgcgtcatc
     1021 aggcgtttca tacagcaatt gtggcgggct gtggttctca ctatttgctg caaatgcgtg
     1081 aacggttgtt tgatctggcg gcgcgttatc gatttatctg gctgcggcga acggtgcttt
     1141 cggtggaaat gctggaggat aaacacgatc agcaccagac cctgactgcg gcggtactgg
     1201 cgcgagatac agcgcgcgtc agtgagttaa tgcgccagca tttactgacg ccaattccca
     1261 ttatccagca ggcgatggct ggcaattaac tactcttccg gaatacgcaa cacttgcccc
     1321 ggataaattt tatccgggct ttttagcatc ggtttattcg cttcgaagat tttattgtac
     1381 agattagcgt taccgtagac ctgtttggaa atggcactca gagtgtcgcc agacttaacg
     1441 gtataaaact ggctggcagt ggctggtgtc gccgttttca cctgatcatc aacactggca
     1501 ataccggaaa tattccccac cgcaacaagg attttctcct tcgcctcctg actcaggccg
     1561 tcaccagtga ccgtcgcttt gccgtcggca atttgaatgt tcactttatc ggcatcaggt
     1621 ataccggttt tgctcagatg ctcctgcacc ttcttcgcct gatcgtcttt atcgtgctga
     1681 cctgtaaccg catcccagag tttttctccg gcatctttca caaaattgaa cagacccata
     1741 gctacctcat tcgttaacgg aaacactcag aaagtgtagc agaggaacgc ggcgcgttaa
     1801 cgccgcgggg gtgagcacag gtttaatcgc gggtttgcac ccagaacgcg tgaatcaaac
     1861 cggggatata cccaagcagt gtcagcagaa tattaataat gaacgcccaa ccgaaccctt
     1921 taccgagcag cacgccgagc ggcggcagaa tgatggtgat gacgattctc cagaaaccca
     1981 tatgtactcc ctataagaaa attactcatt gtttaaaaag agattttatc tcttaagcgt
     2041 agtaatttta gcggagcctg ccagtttcag cttacgcttt tactctcatt tacgctgctg
     2101 aggctggtat atgttgcatt tttagtttag agtttactta atttagaaaa tacttaaata
     2161 aatatgactg aactcgcgca attacaggcc agtaccgaac aggcagcggc cttattgaaa
     2221 gcaatgagcc accctaaacg gttgctgatt ctgtgcatgc ttagcggttc ccccggcacc
     2281 agcgcgggag agctgacgcg cattaccgga ctgagcgcct ctgcgacatc acagcatctc
     2341 gctcgtatgc gggacgaagg gcttatcaac agccaacggg atgcccaacg cattctatat
     2401 tccattaaaa atgaggcggt aaatgccatt atcgccaccc tgaaaaatgt ctattgtccg
     2461 taaggagtca ctatggcttt gacaaccatt tcgccgcatg atgcacaaga attaatcgca
     2521 cgcggcgcaa agttaatcga tattcgtgac gctgatgaat atcttcgtga acatattcct
     2581 gaagcagatc tggctccatt atccgtgctg gaacagtcag gtcttccacc taaattacgt
     2641 cgcgagcaaa ttatattcca ctgccaggca ggtaaacgca ccagtaataa cgccgataaa
     2701 ttagcagcga ttgccgcccc cgcagaaata tttttactcg aagatgggat tgatggctgg
     2761 aaaagagctg gattgccagt agcggtaaat aaatctcaac ccttgccgtt aatgcgccag
     2821 gtgcagatcg ctgcgggtgg tttaatatta atcggcgttg tactgggtta taccgtaaat
     2881 agcggtttct tcttattaag tggctttgtt ggtgccgggt tactgtttgc aggaatcagc
     2941 ggtttttgtg gaatggcaag gttgttagat aagataccgt ggaaccaacg agcttgagaa
     3001 gcgacgccgg acgcgcccta gcagcgacat ccggcctcag taattagatc aggaaatcgt
     3061 cgagagattt accttctgcc agcgcctgag caattggctt cggtgtacgg ccctgaccgg
     3121 tccaggtttt agtttcaccg ttaacatcag tgaatttata tttcgccgga cgcggctggc
     3181 gttttttacc agcacgtggt gcagcagcag agctattacc caataactct tccgggttaa
     3241 ttccgtcagc tttcatcagc tccagccagg tgctaatttt ttcctgacgc tcagccagtt
     3301 cacgctgctg ctgttcttct tcttcacgtc tttctttagt gacaaccctg aatttttcga
     3361 gcatttcttc aagaacgtca atggagaatt cgcgagccat cgcacggagg gtgcgaatgt
     3421 tatttaaact ttgtaacatt acggacataa taaagaaaac cttttaacgc caaaacaaaa
     3481 aagtatttcg tcgcgtagtg taagcgatca cttattatat ttccacaaaa tttaatctat
     3541 ttacagtcct gcgcgagatt atttcagctg ttattttctg aaactctcag aataatttca
     3601 agattatata ttagccattt tgatgagtat tttaggttat tggtgaacag agtggcgctc
     3661 tctgctcgct aactccgtaa gcctgacaaa accgaagtga ataaaaatgc aatataacag
     3721 attaaaagaa aacacccctc actggtgctt tctgctgtta atgtacattt tctggcatca
     3781 aaaatgctgt aaatgacccg gtcacgacaa acaccgaatt ctctacgctt atagcgttac
     3841 ctccccccca aactttcatt agtgaaatta aaggcaaggc catatccaat tgatttaatt
     3901 gaaaataaat catatcggca tgtaaaaatc cacaatgtac aaaaaacgaa ccgttacgga
     3961 atattttatc tacaaaagct gactaaataa aaatttttca ctaatttatt agtcatagcc
     4021 agcgatatac gctatgcgaa aatacagatg gcaatgagat ccactgcttt catctccatt
     4081 aacatcccat tacgctttta ttaaggagca ttagcatgtt ctcaccgcag tcacgcttgc
     4141 gtcatgcagt tgcagatacg ttcgcgatgg ttgtttactg ttctgtcgtg aacatgtgta
     4201 ttgaagtttt cctctccgga atgagcttcg aacagtcttt ttattccaga ttggtagcga
     4261 ttccggtgaa catcttaatt gcatggccat acggtatgta ccgcgatctg tttatgcgcg
     4321 cggcacgcaa aggctggata aaaaatctgg ctgatatcct ggcttatgtg acgttccagt
     4381 caccggtgta tgtggcgatc ttgttagtgg tgggcgcaga ctggcatcag attatggcgg
     4441 cggtcagttc aaacatcgtt gtttcgatgt tgatgggggc ggtttatggc tacttcctcg
     4501 attattgccg ccgactgttt aaagtcagcc gttaccagca ggtaaaagcc tgactcttct
     4561 tttcgcgact ggcatcgcca gtcgcgtctt taactggctt cgccaaataa accattcaaa
     4621 taacgttcaa gcgcaacacg cgaactaaag ccatgcggga taccgtaatg ctcgtgtcgc
     4681 tgtcgctcgc ctgctaaata gagcggaaat tgctggtaat gatcgcaagt tttaatatcg
     4741 gaagcgggtt cagccagtgt tgaactgcgt tcttttaatg ttggatgaat taccagagcg
     4801 gtacgtccca ttcgcgcttc gcgattaaca tagacataat tttccccacg gcggtaacca
     4861 tacgctttct gggttaccac atcgacagta aaaccgactt tttcaagtac gcgcgccacc
     4921 tcgtctggtc gtaaatacat atttgatcct cgttattttc aaccgcgggc ctaccttacc
     4981 tgattgcgca ttcacaacgc tttcagaaaa gtccataaag ccgcgatgaa cctcagttaa
     5041 gaaaattatg gtctacactg aaaattacat cgaattctaa tgggggatca tatgtttaac
     5101 cgaccgaacc gcaacgacgt tgatgatggc gtgcaggata tccagaatga tgtcaatcaa
     5161 ttagctgaca gcctcgaatc tgtattgaag tcctggggca gcgacgccaa aggggaagct
     5221 gaagccgcac gcagtaaagc tcaggcgttg ctgaaagaaa ccagggcgcg aatgcatggt
     5281 cgtactcgcg tccagcaagc cgcgcgcgat gccgttggct gcgcggattc ttttgttcgt
     5341 gaaagaccct ggtgtagcgt gggtacagca gctgcggtag gtatttttat cggcgcacag
     5401 ttaagcatgc gcaaatcgta gtgcaaaaat gataataaat acgcgtcttt gaccccgaag
     5461 cctgtcttcg gggtttcttt ttgcctggtg aatcacaaaa atccccctcc cccgtcacgc
     5521 tcatatccag ggtaatttcg atcactattt gctatatatt gtgtggttga atcttttttc
     5581 aactacatct agtatctctg tatcaacaga gagacaaccc gacgcgtatc atcgcgccgt
     5641 atcttcattt taaacagaaa tacgaatcat gcgcataact atttatactc gtaacgattg
     5701 cgttcagtgc cacgccacca aacgggcgat ggaaaaccgg ggctttgatt ttgaaatgat
     5761 taatgtcgat cgcgttcctg aagcagcgga agcgttgcgt gctcagggct ttcgtcagtt
     5821 gccggtagtg attgctggcg atcttagctg gtctggtttc cgtccggaca tgattaaccg
     5881 tctgcatcca gcgccacacg cggccagtgc atgagccagc tcgtctactt ctccagcagc
     5941 tccgaaaaca cgcagcgttt tatcgaacgt ttaggtctgc ccgcggtgcg catcccgctc
     6001 aatgagcggg aacggattca ggtagacgag ccttacatcc tgatcgtgcc ctcttacggc
     6061 ggcggcggta cggctggcgc ggtgccacga caggtaattc gctttttaaa cgacgagcac
     6121 aaccgggcgt tgcttcgcgg cgttattgct tctggtaatc gcaactttgg tgaggcgtat
     6181 ggccgcgccg gagatgtgat tgcccggaaa tgcggcgtgc cgtggctgta ccgttttgaa
     6241 ctcatgggta cgcaaagcga tatcgaaaac gttcgtaaag gagtaaccga attttggcaa
     6301 cgacaaccgc agaatgcctg acgcaggaaa cgatggatta ccacgcgctg aatgcgatgc
     6361 ttaatcttta cgatagcgca ggtcgcattc agttcgataa agaccgccag gccgttgacg
     6421 cctttattgc gacgcatgtg cgtccgaaca gtgtgacctt cagtagccag cagcagcgcc
     6481 tgaactggct ggtcaacgaa ggttactatg atgaaagcgt tcttaatcgc tactctcgcg
     6541 actttgtcat tacgctgttt gcccacgcac acaccagcgg ttttcgtttc cagacattcc
     6601 tcggggcatg gaagttttac accagctata cgttgaagac attcgacggt aaacgttatc
     6661 tggaagattt tgccgatcga gtcacgatgg tggcgctgac gctggcacaa ggcgatgaga
     6721 cgctggcgtt gcaactgacc gatgaaatgc tgtcaggacg ctttcagcca gccacgccaa
     6781 cattcctcaa ctgcggtaag cagcagcgcg gcgaactggt ttcctgtttt ttgctgcgta
     6841 ttgaagacaa tatggagtcg attggtcggg cggtaaattc cgcactgcag ctgtcgaaac
     6901 gcggcggcgg cgtagcattt ttgctgtcga atctgcggga agcgggcgcg ccaattaaac
     6961 gtattgaaaa tcaatcttct ggcgtaattc cggtgatgaa aatgttggaa gacgcatttt
     7021 cctatgccaa ccaactcggc gcgcgtcagg gggcaggtgc agtctattta catgctcatc
     7081 atcccgatat tctgcgtttt ctcgacacga aacgggaaaa tgccgacgaa aaaatccgca
     7141 ttaaaacact gtcgctgggc gtggtgatcc cggatatcac tttccatctg gcaaaagaga
     7201 atgcgcagat ggcgctgttt tcgccctatg acgttgagcg agtttatggc aagccgtttg
     7261 ccgatatcgc cattagcgaa cactatgacg aactggttgc cgatgaacgc atccgcaaaa
     7321 aatacctcaa cgcccgtgat ttcttccagc gactggcaga aatccagttt gagtccggct
     7381 atccctacat catgtatgaa gacacagtaa accgtgctaa ccctatcgcc gggcgcataa
     7441 atatgagtaa tctctgctca gaaattttgc aggttaacag cgcctcagag tatgacgaga
     7501 atctcgacta tgcccgcaca ggccatgata tttcctgcaa tttaggttcg ttgaatattg
     7561 cgcacaccat ggattccccc gatttttccc gcacggtaga gactgccgtg cgcggtttaa
     7621 cggcggtgtc agatatgagt catatccgca gcgtgccgtc catcgaagcc ggaaatgccg
     7681 cctcgcacgc catcggactg gggcagatga atttacatgg ttatctggcg agggaaggca
     7741 tcgcttatgg ttcgccggaa gccctggatt tcaccaatct ctatttctat accatcacct
     7801 ggcacgcact gcgtacctcg atgttgctgg cgcgcgaacg cggtgaaacc ttcgccgggt
     7861 tcaaacagtc acgctatgcc agtggtgaat atttcagcca atatctgcaa ggtaactggc
     7921 agccgaaaac ggcgaaagtt ggcgaactgt ttgcccgcag cggtattacg ttacctaccc
     7981 gtgagatgtg ggcgcaactg cgcgacgacg tgatgcgcta cggcatatac aaccagaatc
     8041 tccaggcggt gccgccaacc gggtctatct cttatatcaa ccatgctacg tcgagtattc
     8101 atccgattgt ggcgaaagta gagatacgca aagagggcaa aacaggacgc gtttactacc
     8161 ctgccccgtt tatgactaac gaaaatctgg cgctgtatca ggacgcttac gaaattggcg
     8221 cagaaaagat catcgacacc tacgcggaag cgactcgcca tgtcgatcag gggctgtcgc
     8281 tgacgctgtt tttccccgat accgccacca ctcgcgatat caacaaagcg cagatttacg
     8341 cctggcgcaa aggtatcaaa acgctctatt acatccgcct gcgtcagatg gcgctggaag
     8401 gcactgaaat tgaaggctgc gtctcctgtg cactttaagg aatatctatg aaactctcac
     8461 gtatcagcgc catcaactgg aacaagatat ctgacgataa agatctggag gtgtggaatc
     8521 gcctgaccag caatttctgg ctaccagaaa aggtgccgct gtcgaacgat attcccgcct
     8581 ggcagacatt aactgtcgta gaacaacaac tgacgatgcg cgtttttact ggcctgacgc
     8641 tgctcgacac gctgcaaaat gttatcggcg cgccttcgct gatgcccgat gcgctcacgc
     8701 ctcatgaaga ggcggtatta tcgaatatca gctttatgga agcggttcat gcccgctctt
     8761 acagttcgat tttctcgacg ctgtgccaga ccaaagatgt cgatgccgcc tacgcctgga
     8821 gtgaagaaaa cgcaccgttg cagcgaaaag ctcagattat tcagcaacat tatcgcggtg
     8881 atgatccgct gaaaaagaaa atcgccagtg tgtttcttga atcttttttg ttctattccg
     8941 gtttctggct gccgatgtat ttttccagcc gcggaaagct gaccaatacc gcggacctga
     9001 tccgtctgat tatccgcgat gaagcagtcc acggttacta cataggctat aaatatcaga
     9061 aaaacatgga aaagatatct ctgggacaac gtgaagagtt gaagagtttc gccttcgatt
     9121 tgttgctgga actctacgac aacgagttgc aatacaccga tgagctgtac gccgaaaccc
     9181 cgtgggctga cgatgtgaaa gcgtttctct gttacaacac caataaggct ttgatgaatc
     9241 tgggctacga accgttattt cccgcagaaa tggcggaagt gaatccggca atcctcgccg
     9301 cgctttcgcc gaatgccgat gaaaatcacg atttcttttc cggttcaggc tcctcttatg
     9361 tgatggggaa agcggttgaa acagaagatg aagactggaa tttctgaggg tgttattttc
     9421 aaaaatatca ctacccgcag cagggaaata attcccgcca aatagctttt tatcacgcaa
     9481 atattctgta gtgatctaca ctgatactct gttgcattat tcgcctgaaa ctacaatatt
     9541 caggcgtttt ttcgctatct atgacaaaaa atatcaactt ttctcgattt gctctcagcc
     9601 cttatatcac ggaaaattcc ggcgatttgc tcgcatcaat attcatgcca catttgccct
     9661 caggggttgc ctcagattct cagtatgtta gggtagaaaa aagtgactat tcccattggg
     9721 taatatatcg acataaacaa ataaaggaat cattctattg catggccatt aaattagaaa
     9781 ttaaaaatct ttataaaata tttggtgagc atccacagcg agcgttcaaa tatatcgaac
     9841 aaggactttc aaaagaacaa attctggaaa aaactgggct atcgcttggc gtaaaagacg
     9901 ccagtctggc cattgaagaa ggcgagatat ttgtcatcat gggattatcc ggctcgggta
     9961 aatccacaat ggtacgcctt ctcaatcgcc tgattgaacc cacccgcggg caagtgctga
    10021 ttgatggtgt ggatattgcc aaaatatccg acgccgaact ccgtgaggtg cgcagaaaaa
    10081 agattgcgat ggtcttccag tcctttgcct taatgccgca tatgaccgtg ctggacaata
    10141 ctgcgttcgg tatggaattg gccggaatta atgccgaaga acgccgggaa aaagcccttg
    10201 atgcactgcg tcaggtcggg ctggaaaatt atgcccacag ctacccggat gaactctctg
    10261 gcgggatgcg tcaacgtgtg ggattagccc gcgcgttagc gattaatccg gatatattat
    10321 taatggacga agccttctcg gcgctcgatc cattaattcg caccgagatg caggatgagc
    10381 tggtaaaatt acaggcgaaa catcagcgca ccattgtctt tatttcccac gatcttgatg
    10441 aagccatgcg tattggcgac cgaattgcca ttatgcaaaa tggtgaagtg gtacaggtcg
    10501 gcacaccgga tgaaattctc aataatccgg cgaatgatta tgtccgtacc ttcttccgtg
    10561 gcgttgatat tagtcaggtg ttcagtgcga aagatattgc ccgccggaca ccgaatggct
    10621 taattcgtaa aactcctggc ttcggcccac gttcggcact gaaattattg caggatgaag
    10681 atcgcgaata tggctacgtt atcgaacgcg gtaataagtt tgtcggcgca gtctccatcg
    10741 attcgcttaa aaccgcgtta acgcagcagc aaggtcttga tgcggcgctg attgatgcgc
    10801 cgttagcagt cgatgcacaa acgcctctta gcgagttgct ctctcatgtc ggacaggcac
    10861 cctgtgcggt gcccgtggtc gacgaggacc aacagtatgt cggcatcatt tcgaaaggaa
    10921 tgctgctgcg cgctttagat cgtgaggggg taaataatgg ctgatcaaaa taatccgtgg
    10981 gataccacgc cagcggcgga cagtgccgcg caatccgcag acgcctgggg gacaccgacg
    11041 actgcaccgg ctgacggcgg tggtgctgac tggctgacca gtacgcctgc gccaaacgtc
    11101 gagcatttta atattctcga tccgttccat aaaacgctga tcccgctcga cagttgggtc
    11161 actgaaggga tcgactgggt tgttacccat ttccgtcccg tcttccaggg cgtgcgcgtt
    11221 ccggttgatt atatcctcaa cggtttccag caattgctgc tgggtatgcc agcgccagtg
    11281 gcgattatcg ttttcgctct catcgcctgg cagatttccg gggtcggaat gggcgtggcg
    11341 acgctggtct cgctgattgc catcggcgca atcggtgcct gttcgcaggc aatggtgact
    11401 ctggcgctgg tgttaactgc cctgctgttc tgtatcgtca tcggtttgcc gttggggata
    11461 tggctggcga gaagtccgcg agcggcgaaa attattcgtc cactgcttga tgccatgcag
    11521 accacgccag cgtttgttta tctggtgcca atcgtcatgc tatttggtat cggtaacgtg
    11581 ccgggcgtgg tggtgacgat catctttgcg ctgccgccga ttatccgtct gaccattctg
    11641 gggattaacc aggttccggc ggatctgatt gaagcctcgc gctcattcgg tgccagcccg
    11701 cgccagatgc tgttcaaagt tcagttaccg ctggcgatgc cgaccattat ggcgggcgtt
    11761 aaccagacgc tgatgctggc cctttctatg gtggtcattg cctcgatgat tgccgtcggc
    11821 gggctgggtc agatggtact tcgcggtatc ggtcgtctgg atatggggct tgccaccgtt
    11881 ggcggcgtcg ggattgtgat cctcgccatt atcctcgatc gcctgacgca ggccgttggg
    11941 cgcgactcac gcagtcgcgg caaccgtcgc tggtacacca ctggccctgt tggtctgctg
    12001 acccgcccat tcattaagta actctgcact tgcccggtga cgccgggcat tatcaccctg
    12061 ccaaaaaaag gaataacaat gcgacatagc gtactttttg cgacagcgtt tgccacgctt
    12121 atctctacac aaacttttgc tgccgatctg ccgggcaaag gcattactgt taatccagtt
    12181 cagagcacca tcactgaaga aaccttccag acgctgctgg tcagtcgtgc gctggagaaa
    12241 ttaggttata ccgtcaacaa acccagcgaa gtggattaca acgttggcta cacctcgctt
    12301 gcttccggcg atgcaacctt caccgccgtg aactggacgc cactccatga caacatgtac
    12361 gaagctgccg gtggcgataa gaaattttat cgtgaagggg tatttgttaa cggcgcagca
    12421 cagggttacc tgatcgataa gaaaaccgcc gaccaataca aaatcaccaa catcgcacaa
    12481 ctgaaagatc cgaagatcgc caaactgttc gataccaacg gcgacggtaa agcggattta
    12541 accggttgta accctggctg gggctgcgaa ggtgcgatca accaccagct tgccgcgtat
    12601 gaactgacca acaccgtgac gcataatcag gggaactacg cagcgatgat ggccgacacc
    12661 atcagtcgct acaaagaggg caaaccggtg ttttattaca cctggacgcc gtactgggtg
    12721 agtaatgagc tgaagccagg gaaagatgtg gtctggttgc aggtgccgtt ctccgcactg
    12781 ccgggcgata aaaatgccga taccaaactg ccgaatggcg cgaattacgg cttcccggtc
    12841 agcaccatgc atatcgttgc caacaaagcc tggaccgaga aaaatccggc ggcagccaaa
    12901 ctgtttgcca ttatgcagtt gccagtggcc gatattaacg cccagaacgc cattatgcat
    12961 gacggcaaag cctcagaagg cgatattcag ggccacgttg atggctggat caaagcccac
    13021 cagcagcagt tcgatggctg ggtgaatgag gcgctggcag cgcagaagta atttttattc
    13081 gggcggataa ggcgttttcg cctcatccgc cgctctgtac acaatgcctg atgcgacgct
    13141 gacgcgtcct atcatgccta caaaccacat cgcacatttc aacaagctat ctttcatccc
    13201 atattcatca acatccgcta ttattgattt ccagcttaat catcacctga tgaacaaaaa
    13261 taatgactaa acctaatcat gagcttagcc cggcgctgat cgtgctgatg tctatcgcca
    13321 ccggtctggc ggtagccagt aactattacg cccagccatt gctcgacacc atcgcgcgta
    13381 acttttccct ttccgccagt tcggcaggct ttattgttac cgccgcgcag ttgggctatg
    13441 ccgcaggtct actgtttctt gttcccctcg gtgatatgtt tgaacgccgc cgcctgattg
    13501 tctcgatgac cttactggca gcgggcggta tgttaatcac tgccagcagt cagtcactgg
    13561 cgatgatgat cctcggtacg gcattaaccg gtttattctc agtcgtggca caaattctgg
    13621 ttccgctggc agcgacgctg gcttcaccgg ataaacgcgg caaagtggtt ggcaccatta
    13681 tgagcggtct gctgttgggg atattgctgg cacgaaccgt tgccgggtta ctggcaaacc
    13741 tcggcggctg gcgcaccgtc ttttgggttg cttcggtgtt aatggcactg atggcgctgg
    13801 cattatggcg tggtctgcca caaatgaaat cagaaaccca cctcaactac ccacagttgt
    13861 tgggttccgt tttcagcatg ttcatcagcg ataagatcct gcgcacccgc gcgttgctgg
    13921 gctgcatgac ctttgccaac ttcagcattc tctggacctc aatggccttt ttgcttgccg
    13981 ctccaccttt taactacagc gatggtgaca ttggtctgtt tgggcttgcg ggagctgccg
    14041 gagcgttggg cgctcgtccg gcgggcggtt ttgccgacaa gggcaaatcg caccacaact
    14101 ttcggtctgc ttctgctgtt actttcatgg ctggcaatct ggtttggtca cacttccgta
    14161 ctggcgttga ttatcggcat tctggtgctg gacctcaccg tacagggcgt gcatatcact
    14221 aaccagacgg taatttatcg aatacatcct gatgcgcgta atcgcctgac cgcaggttac
    14281 atgaccagct actttattgg cggtgccgcc ggttcgctaa tttcagcctc agcctggcaa
    14341 catggcggtt gggctggcgt ttgtctggct ggcgcgacga ttgccctggt taacttactg
    14401 gtctggtggc gaggttttca tcgtcaggaa gccgcaaatt aagcaatcag caaccgtttt
    14461 cgtggcttta cacttataag ggtgttaaga agcccatcag tctgataagg ttaagatatt
    14521 cattcagtct atttataata ttaacaatcg ttaagcgtac actctatgga aagccctact
    14581 ccacagcctg ctcctggttc ggcgaccttc atggaaggat gcaaagacag tttaccgatt
    14641 gttattagtt atattccggt ggcctttgcg ttcggtctga atgcgacccg tctgggattc
    14701 tctcctctcg aaagcgtttt tttctcctgc atcatttatg caggcgcgag ccagttcgtc
    14761 attaccgcga tgctggcagc cgggagtagt ttgtgggttg ctgcactgac cgtcatggca
    14821 atggatgttc gccatgtttt gtatggcccg tcactgcgta gccgtattat tcagcgtctg
    14881 caaaaatcga aaactgccct atgggcgttt ggcctgacgg atgaggtttt tgccgccgcc
    14941 accgcaaaac tggtacgcaa taatcgccgc tggagcgaga actggatgat cggcattgcc
    15001 ttcagttcat ggtcatcgtg ggtatttggt acggtaatag gggcattctc cggcagcggc
    15061 ttgctgcaag gttatcccgc cgttgaagct gcattaggtt ttatgcttcc ggcactcttt
    15121 atgagtttcc tgctcgcctc tttccagcgc aaacaatctc tttgcgttac cgcagcttta
    15181 gttggtgccc ttgcaggcgt aacgctattt tctattcccg tcgccattct ggcaggcatt
    15241 gtctgtggct gcctcactgc gttaatccag gcattctggc aaggagcgcc cgatgagcta
    15301 tgaggttctg ctgcttgggt tactggttgg cgcggcgaat tattgcttcc gctatttgcc
    15361 gctgcgcctg cgtgtgggta atgcccgccc aaccaaacgt ggcgcggtag gtattttgct
    15421 cgacaccatt ggcatcgcct cgatatgcgc tctgctggtt gtctctaccg caccagaagt
    15481 gatgcacgat acacgccgtt tcgtgcccac gctggtcggc ttcgcggtac tgggtgccag
    15541 tttctataaa acacgcagca ttatcatccc aacactgctt agtgcgctgg cctatgggct
    15601 cgcctggaaa gtgatggcga ttatataacc cacaagaatc atttttctaa aacaatacat
    15661 ttactttatt tgtcactgtc gttactatat cggctgaaat taatgaggtc atacccaaat
    15721 ggatagttcg tttacgccca ttgaacaaat gctaaaattt cgcgccagcc gccacgaaga
    15781 ttttccttat caggagatcc ttctgactcg tctttgcatg cacatgcaaa gcaagctgct
    15841 ggagaaccgc aataaaatgc tgaaggctca ggggattaac gagacgttgt ttatggcgtt
    15901 gattacgctg gagtctcagg aaaaccacag tattcagcct tctgaattaa gttgtgctct
    15961 tggatcatcc cgtaccaacg cgacgcgtat tgccgatgaa ctggaaaaac gcggttggat
    16021 cgaacgtcgt gaaagcgata acgatcgccg ctgcctgcat ctgcaattaa cggaaaaagg
    16081 tcacgagttt ttgcgcgagg ttttaccacc gcagcataac tgcctgcatc aactctggtc
    16141 cgcgctcagc acaacagaaa aagatcagct cgagcaaatc acccgcaaat tgctctcccg
    16201 tctcgaccag atggaacaag acggtgtggt tctcgaagcg atgagctaac gcgtcatctc
    16261 gctcaaaaat ccagatttat aaaagaaaaa atgaccggcc agcatcgcaa catgctggcc
    16321 tttttggcaa gcaggtcggc tcagccgatg agttaagaag atcgtggaga acaatatgag
    16381 cgcaaatgcg gagactcaaa ccccgcagca accggtaaag aagagcggca aacgtaagcg
    16441 tctgctcctc cttctcacct tgctctttat aattattgcc gtagcgatag ggatttattg
    16501 gtttttggta ctgcgtcatt tcgaagaaac cgatgacgca tacgtggcag ggaatcagat
    16561 tcaaattatg tctcaggtgt ctggcagcgt gacgaaagtc tgggccgata acaccgattt
    16621 tgtaaaagaa ggcgatgttc tggtcactct cgacccgaca gatgctcgcc aggcgtttga
    16681 aaaagccaaa actgcactgg cttccagcgt tcgccaaacc caccagctga tgattaacag
    16741 caaacagttg caggcgaata ttgaggtgca aaaaatcgcc ctcgcgaaag cacaaagcga
    16801 ctacaaccgc cgtgtgccgc tgggcaatgc caacctgatt ggtcgcgaag agctgcaaca
    16861 cgcccgcgac gccgtcacca gtgcccaggc gcaactggac gtcgctattc aacaatacaa
    16921 tgccaatcag gcgatgattc tggggactaa actggaagat cagcctgccg tgcaacaggc
    16981 tgccaccgaa gtacgtaacg cctagctggc gctggagcgt acccgtattg tcagtccgat
    17041 gaccggttat gtttcccgtc gtgccgtgca accgggtgcg caaattagcc caacgacgcc
    17101 gctgatggcg gtcgttccag ccaccaatat gtgggtggat gccaacttta aagagacgca
    17161 gattgccaat atgcgcatcg gtcagccggt caccatcacc accgatattt acggcgatga
    17221 tgtgaaatac accggtaaag tggttggtct ggatatgggc acaggtagcg cgttctcact
    17281 gcttccggcg caaaatgcga ccggtaactg gatcaaagtc gttcagcgtc tacctgtgcg
    17341 tatcgaactg gaccagaaac agctggagca atacccgctg cgcattggtt tgtccacgct
    17401 ggtgagcgtc aataccacta accgtgacgg tcaggtactg gcaaataaag tacgttcaac
    17461 accggtcgcg gtaagtaccg cgcgtgaaat cagcctagca cctgtcaata aactgatcga
    17521 cgatatcgta aaagctaacg ctggctaatc cagaggtgcg tgtgatgcaa cagcaaaaac
    17581 cgctggaagg cgcgcaactg gtcattatga cgattgcgct gtcactggcg acattcatgc
    17641 aggtgctgga ctccaccatt gctaacgtgg cgatccccac tatcgccggg aatctgggct
    17701 catcgctcag ccaaggaacg tgggtaatca cttctttcgg ggtggcgaat gccatctcga
    17761 tcccgcttac cggctggctg gcaaagcgcg tcggggaagt gaaactgttc ctttggtcca
    17821 ccatcgcctt tgctattgcg tcgtgggcgt gtggtgtctc cagcagcctg aatatgctga
    17881 tcttcttccg cgtgattcag gggattgtcg ccgggccgtt gatcccgctt tcgcaaagtc
    17941 tattgctgaa taactacccg ccagccaaac gctcgatcgc gctggcgttg tggtcgatga
    18001 cggtgattgt cgcgccaatt tgcggcccga tcctcggcgg ttatatcagc gataattacc
    18061 actggggctg gatattcttc atcaacgtgc cgattggcgt ggcggtggtg ttgatgacac
    18121 tgcaaactct gcgcggacgt gaaacccgca ccgaacggcg gcggattgat gccgtggggc
    18181 tggcactgct ggttattggt atcggcagcc tgcagattat gctcgaccgc ggtaaagagc
    18241 tggactgttt ttcatcacag gaaattatca ttcttaccgt ggtggcggtg gtggctatct
    18301 gcttcctgat tgtctgggag ctgaccgacg ataacccgat agtcgatctg tcgttgttta
    18361 agtcgcgcaa cttcaccatc ggctgcttgt gtatcagcct cgcgtatatg ctctacttcg
    18421 gcgctattgt tctgctgccg cagttgttgc aggaggtcta cggttacacg gcgacctggg
    18481 caggtttggc ctctgcgccg gtagggatta ttccggtgat tctgtcgccg attatcggcc
    18541 gcttcgcgca taaactggat atgcggcggc tggtaacctt cagctttatt atgtatgccg
    18601 tctgcttcta ctggcgtgcc tatacctttg aaccaggtat ggattttggc gcgtcggcct
    18661 ggccgcagtt tatccagggg tttgcggtgg cctgcttctt tatgccgctg accaccatta
    18721 cgctgtctgg tttgccaccg gaacgactgg cggcggcatc gagcctctct aactttacgc
    18781 gaacgctggc ggggtctatc ggcacgtcga taaccacgac catgtggacc aaccgcgagt
    18841 cgatgcacca tgcgcagttg actgagtcgg taaacccgtt caacccgaat gcccaggcga
    18901 tgtacagtca actggaaggg cttgggatga cgcaacagca ggcatcaggc tggattgccc
    18961 agcagatcac caatcagggg ctgattattt ccgccaatga gatcttctgg atgtcagccg
    19021 ggatattcct cgtactgctg gggctggtgt ggtttgctaa accgccattt ggcgcaggtg
    19081 gcggcggagg cggtgcgcac taagtacaac taagccagtt catttgaact ggcttttttc
    19141 aattaattgt gaagatagtt tactgactag atgtgcagtt cctgcaactt ctctttcggc
    19201 agtgccagtt cttcgttgct gttgatgcgt acgtcacgtt ccagaatgct acgcgcaata
    19261 tcctgcgctt cctgcaacga gtgcatctgg taagtgccac actggtagac gttcagttcc
    19321 gggatctgat tctgatcctg cactttcagc acgtcttcca ttgccgcttt ccaggcatca
    19381 gcaacacgct gctcatctgg cgtaccaatc agactcatat aaaaaccggt gcggcagccc
    19441 attggcgaga tatcgataat ctctacacca ttaccgttaa gatggttacg cataaaacca
    19501 gcaaacaggt gctccagggt atggatccct ctttctggca tcacttcttt gttcggcacg
    19561 cagaagcgca gatcgaacac ggtgattgcg tcgccatgcg gggtgttcat tgttttcgcc
    19621 acccgaactg caggcgcttc catccgggta tgatcgactg tgaagctatc taacaacggc
    19681 atttagccac ctccggtaat ttttttaaaa attttctgaa ctctttcttc ccaggcgagt
    19741 ctgagtatat gaaagacgcg catttgttat catcatccct gaattcagag atgaaatttt
    19801 ggccactcac gagtggcctt tttcttttct gtcaggcgtg tttttccagc cacaccgcaa
    19861 acggttcggt atcagcggtt tccatttcct gctgacggcg ttcagatgcc tcgcgctcgg
    19921 ctacaaaatc ctcttcgcgc agaatttcca gcggctcttc acgcagcaga ttacggtagg
    19981 cttctgcaaa cgctttgcct gttccgccaa taccagtatc aatcatagac cttaagatac
    20041 gggcagagaa agtcagatcg ggattatcga agcaggcaac cagttcatca cacactttct
    20101 gatacgcttc gccgccgtta atgctatcca gcgtttgcgc gacgcgtttc agatcgtgga
    20161 acagatcttt acccacctgc agtaacggga actgcgcggt ttcgcagccg atacccagcg
    20221 tcaggcccgg tttgcgacct tcaaggatca cccggttcca gttaacgcgc gtacaggcaa
    20281 gttcgctact gctcatttcc ggtgcatcag ccagcgcaca ccagaccata aacaggtcga
    20341 ggaatcgcac ctgctgttca tctacaccaa tcggcgagaa cgggttgatg tccagcgaac
    20401 gcacttcaat atattcaatg ccgccacgta acagcgcatc agaaggtgac tcgccgctgc
    20461 gggtaacgcg ttttggacga atcggcgcat acagttcgtt ttcaatctgc agcacgttgc
    20521 tattgatttg cagcctctta ccgtctttct caataccaat cttcgcgtac tcttccgatg
    20581 gcgttttgat tgcctgttta aggcccgcta cgtactcgta aagatcgttg aaggtaatac
    20641 caagattgct ttgcgattta ttggtatagc cgagatcgct caaacgaaga gaggtcgcat
    20701 acggcaggta atacataccg cactcggttt tctcaaacgg cagcgacgtt ggttttcctt
    20761 gcaggaaaga agaacaaatc gccggagatg caccaaacag ataaggaatg acccaaccga
    20821 aacgatagta attgcggata acgcggaaat agcccgcaga aattttctct ttggcatcag
    20881 cgcccgagat atcaccgcac ttcgcttgcc agaatgccat tggcaaagag aaattgtagt
    20941 gcacgccgga aatggtttgc atcagcgcgc cgtagcgatt tttcagccct tcacgataca
    21001 gcgttttaaa gcgtccggtg ttagaagtgc cgtactgtgc cagttcgatg tcctgacctt
    21061 ctgcgatgta gcatggcata cttaacggcc acatccgctc atcgcccata ttgcgcgccg
    21121 tataacgatg cagatcgcgc ataaaggtca gcatatgttc aatatcacca tccactggtg
    21181 taatgaattc cagcaatgct tccgcaaaat cggtagtaat ccatttgtgc gtcagtgcgg
    21241 aacctaatgc ttcaggatga cctgttgttg ccagtgtgcc atcagcatta acacgcaaag
    21301 tttcgcgctc cagcccacgc tgtatcccct ttaacgcctg aggatgtttt tccagccagg
    21361 ccagcgcctg tgatacgtcc gggatcaaat tgacctcccg cctgtcaaaa tcgttttaat
    21421 tagcataact gtaatggtga ccatatgtgc aggcctacaa ttagtgccac cacatcatgc
    21481 cctgaacggt cgctgctgca actgcaacat agcgtaacgc tttaccaagg cacaaaaaaa
    21541 agattactgg cccccaggag atgcgcatcc atcccgctaa cagacacagt aaatcgccaa
    21601 ccacgggcat ccagcttaat aatagcgtga ctgcaccata gcgtttcagc cagccggtag
    21661 ctttctcttg ccagcgcgat gttttacgca atggaaagaa acgcccaagg ataacgttag
    21721 ttaaccctcc aaggctatta cccattgttg ctgttaaaac taaaacctag ggatgactga
    21781 tcccggaaag caacattgcc accagcacga cttcggagtt gccgggtaat agtgtagcgc
    21841 tgagaaaact actggcaaac aacgagaaaa gcgataacgc ttcactcaca gcaagcgaac
    21901 atccacagcg tccatgcctg ctgcacgggc cgcctgaata ccgaaatcgg catcttcaaa
    21961 gaccacacac tgcgtcggtt gcacgcccat acgctgcgcg cacaacaaaa atgtgtctgg
    22021 cgcgggttta tggtgtttga cgtgatcagc agcgacgacg gcgtcaaaat aacggcgtaa
    22081 tcccaggtgc gccagcaatg cctctgcgat ggcgctttca ctccccgttc ctacagccat
    22141 tgggcgacga ccatgccaac ttttcaccac ttcaacaaga ggaagcggct cgacactatc
    22201 cagcagcata cttcttactg cttctgtttt ttcacgcgct aacgcatgcg ggtcgagatc
    22261 ggcctgattc agctcaataa ttgcctgagc aatacgccag gtgggcgatc cattaagcgc
    22321 aatcatcgcc tgaatatcgt actgaagacc gtagtgccct aatacttcgc gccacgcttt
    22381 acggtgcgta ggctccgtat ccaggattgt gccatccata tcaaaaatta aacctgcata
    22441 acgctcgtac atggtcttct cgcaagtcga aaaatatgac gctactttag cgtaattgct
    22501 tgattttgtc gctgatggcg aaggagaaga aaattcagga aatggataaa gtagttattt
    22561 agaaatgaga tatttttgaa ggaaattttt tggagaa
//
LOCUS       DAJVQH010000059        22357 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJVQH010000059 DAJVQH010000000
VERSION     DAJVQH010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.059"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(22..693)
                     /gene="queE"
                     /locus_tag="OQM87_002306"
     CDS             complement(22..693)
                     /gene="queE"
                     /locus_tag="OQM87_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCS1508422.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            832..972
                     /locus_tag="OQM87_002307"
     CDS             832..972
                     /locus_tag="OQM87_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508423.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            986..1858
                     /locus_tag="OQM87_002308"
     CDS             986..1858
                     /locus_tag="OQM87_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCS1508424.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKNETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1918..3216)
                     /gene="eno"
                     /locus_tag="OQM87_002309"
     CDS             complement(1918..3216)
                     /gene="eno"
                     /locus_tag="OQM87_002309"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCS1508425.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3304..4941)
                     /gene="pyrG"
                     /locus_tag="OQM87_002310"
     CDS             complement(3304..4941)
                     /gene="pyrG"
                     /locus_tag="OQM87_002310"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCS1508426.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5169..5960)
                     /gene="mazG"
                     /locus_tag="OQM87_002311"
     CDS             complement(5169..5960)
                     /gene="mazG"
                     /locus_tag="OQM87_002311"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCS1508427.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6056..8290)
                     /gene="relA"
                     /locus_tag="OQM87_002312"
     CDS             complement(6056..8290)
                     /gene="relA"
                     /locus_tag="OQM87_002312"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HCS1508428.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(8338..9639)
                     /gene="rlmD"
                     /locus_tag="OQM87_002313"
     CDS             complement(8338..9639)
                     /gene="rlmD"
                     /locus_tag="OQM87_002313"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCS1508429.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9696..12452
                     /gene="barA"
                     /locus_tag="OQM87_002314"
     CDS             9696..12452
                     /gene="barA"
                     /locus_tag="OQM87_002314"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCS1508430.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12684..14024)
                     /gene="gudD"
                     /locus_tag="OQM87_002315"
     CDS             complement(12684..14024)
                     /gene="gudD"
                     /locus_tag="OQM87_002315"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1508431.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14045..15385)
                     /locus_tag="OQM87_002316"
     CDS             complement(14045..15385)
                     /locus_tag="OQM87_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1508432.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15387..16739)
                     /gene="gudP"
                     /locus_tag="OQM87_002317"
     CDS             complement(15387..16739)
                     /gene="gudP"
                     /locus_tag="OQM87_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCS1508433.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17173..17622)
                     /locus_tag="OQM87_002318"
     CDS             complement(17173..17622)
                     /locus_tag="OQM87_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCS1508434.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17640..18422)
                     /gene="truC"
                     /locus_tag="OQM87_002319"
     CDS             complement(17640..18422)
                     /gene="truC"
                     /locus_tag="OQM87_002319"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCS1508435.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTVHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18422..18751)
                     /locus_tag="OQM87_002320"
     CDS             complement(18422..18751)
                     /locus_tag="OQM87_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCS1508436.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19373..19918)
                     /gene="syd"
                     /locus_tag="OQM87_002321"
     CDS             complement(19373..19918)
                     /gene="syd"
                     /locus_tag="OQM87_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCS1508437.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRMHLASNLAEFLNQLKPLL"
     gene            19986..20834
                     /gene="queF"
                     /locus_tag="OQM87_002322"
     CDS             19986..20834
                     /gene="queF"
                     /locus_tag="OQM87_002322"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCS1508438.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20946..22310
                     /gene="ppnN"
                     /locus_tag="OQM87_002323"
                     /gene_synonym="ygdH"
     CDS             20946..22310
                     /gene="ppnN"
                     /locus_tag="OQM87_002323"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCS1508439.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5522 a         5925 c         5302 g         5608 t
ORIGIN      
        1 ataacgctta taaatgtgta atcaggcaat atttaaatat ttatgtgttt gcatcgacaa
       61 acgccaatta cgcgcaatac aggtttcaat gcacaatcgt gtggcatcat ccttttggct
      121 aatcggctgc agtgcaatga ctcgcggttt atcatcggtc agcgttgcca gtagttcatc
      181 cagtgcttca atatcgcgta cgcgccccac tggatgcttg atttcgttgg ctcgctccag
      241 tgcctgtgac aacacttcat agccgccgcg catgttcagc tttggcgata cggtaaccca
      301 ggtattcggt gtgcagcgta cttcatgagt tccactggtt tcgatctggc agctaaaacc
      361 gttcttttcg agcaggtcag tcagtggcag caaatcatga atgcaaggct caccacccgt
      421 aatcaccaca tgccgcgcgg tatatccctg acgaccaatg acagccagca agtcttcact
      481 gctcgccgcc ccccacttat cactctcttt ggtcttcgcc agaatgctga aaagggagac
      541 ttcccgatcc tcaagctttt cccaggtgtg tttggtgtcg caccaggcac agccaaccgg
      601 gcatccctgt aaacgaataa aaatggcggg gacgccggta aagtaaccct caccttgcag
      661 ggtctggaac atctcgttaa tcgggtactg catagcattc tctgtgaagt ggataattgt
      721 taattattgc agatcctgcc gcaacaatca tgtcttatta acattctgtt acatgcagat
      781 ttaaagaaaa atacgaaaca tatcgttaat aattaaaggg agtaacgtat tatgtcagaa
      841 gaaaataaag aaaatggatt taatcatgtc aaaatattca ccaaaattat atttattttt
      901 tctgtattag tttttaatga taacgaatct aaaattaccg atgccgccgt caatttattt
      961 atccagattt aatgagaaat ttataatgcg ctatttcata ctaatgttca ctttcgtatg
     1021 ttcctttgtt gcagcccaac caactattgt cccacaatta cagcaacaag ttacgggtct
     1081 tacgagtagc ttaaactcac aagaaaagaa agaactgacg cacaagttag aatctatttt
     1141 caataatacc caagtgcaaa ttgctgtatt aattgtttcc acaaccaaaa atgagacaat
     1201 tgaacaatat gctacaagag tttttgacaa ttggcgttta ggagatgcca aacgtaatga
     1261 tgggatactg atcgttgttg cctggtcgga tcgcactgtc cgcatccagg taggttatgg
     1321 gctggaagaa aaggtaaccg atgctctggc tggagatatc atccgtagca acatgatacc
     1381 cgcatttaaa caacaaaaat tagctaaggg attagagtta gctataatcg ctttgaataa
     1441 ccaactcact tcacaacacc aatatccggc taatccttca gaaagtgaat caacgtcttc
     1501 cactgatcat tattactttg ctattttttg ggtatttgca gtgatgttct tccctttctg
     1561 gttttttcat caaggcagta atttttgtcg cgcatgtaaa agtggagtct gtatttcagc
     1621 aatccatctt ttagatttat tcctgttctc cgataaaact ttttccattg ctgtattttc
     1681 cttctttttt actttcacca tatttatggt ctttacctgt ttatgtgtac ttcagaaaag
     1741 agcatctggt agaagttatc attcagacaa cagcggttcc gctggaggct caagtggcgg
     1801 cggatttagc ggcgggggag gttcttctgg cggtggcggc gcatccggcc gctggtaact
     1861 ccaccataaa aaatgccagc ccgaaggctg gcattatcaa atcagataaa gtcagactta
     1921 tgcctggcct ttgatctctt tacgaccgtt gtacggtgct ttttcgccca gagcttcttc
     1981 gatacgaatc agctggttgt atttagcaac acggtcagaa cggctcatag aaccagtttt
     2041 gatctggcct gcagcagtac caacagccag atcagcgatg gtagcatctt cagtttcacc
     2101 agaacggtga gagataactg cagtgtagcc agcatctttc gccatcttga ttgcagccag
     2161 agtttcggtc agagaaccga tctggttgaa tttgatcagg atggagttag cgataccttt
     2221 ttcgatacct tctttcagga tcttggtgtt ggttacgaac aggtcgtcac caaccagctg
     2281 gattttgtcg cccagaactt tggtctggta tgcgaaaccg tcccagtcag attcgtccag
     2341 accgtcttcg atagaaacga tcgggtactg tttggtcagt tcttccagga agtgagtgaa
     2401 ttcttcagag gtgaacgctt tgttgccttc gccagccaga acgtatttac catctttgta
     2461 gaattcagaa gctgcgcagt ccatcgccag agtgatgtct ttgcccagtt cataaccagc
     2521 agctttaaca gcttcagcga taacagccag agcttcagcg ttggaaccca ggttcggcgc
     2581 atagccacct tcgtcaccaa cagcagtgtt catgcctttc gctttcagaa cttttgccag
     2641 gtgatggaaa acttcagaac ccatgcggat ggcttctttc acagttttcg cgccaaccgg
     2701 ctgaatcatg aattcctgga tatcaacgtt gttgtcagcg tgctcaccac cgttgatgat
     2761 gttcatcatc ggaaccggca tggagtattt gcccggagtg ccgttcagtt cagcgatgtg
     2821 ctcgtacagc ggcatacctt tagcagctgc agcagctttg gcgttagcca gagatacagc
     2881 caggattgcg ttcgcgccga atttggattt gttttcggtg ccgtccaggt cgatcatgat
     2941 cttgtcaatg ccagcctgat ctttagcatc tttgccaatc agcgcctgag cgatcgggcc
     3001 gtttaccgca gcaacagctt tggttacgcc tttacccagg aaacgggatt tgtcgccatc
     3061 gcgcagttcc agagcttcac gggaaccagt agaagcacct gacggagcag ctgccatacc
     3121 gacgaaacca ccctccagat gtacttcggc ttcaacagtc gggttaccac gggagtcgat
     3181 gatttcacga ccgatgattt ttacgatttt ggacattagg ttttcctcaa gtcactagtt
     3241 aaactgaaac tccagacaaa caacgcgtac ccaaggtacg cgttgccgtt ctaacttttt
     3301 tacttacttc gcctgacgtt tctggaactc gctggcggct ttcacaaagc ctgcaaacag
     3361 cgggtgacca tcacgtggag tagaagtaaa ctccggatgg aactggcaag ccacgaacca
     3421 cgggtgattc ggaacttcga tgatctcgac caactgatca tccccggaac ggcccacaac
     3481 gcgcagacct gcatcttcaa tctgtttcaa cagcatgttg ttgacttcgt aacggtgacg
     3541 atgacgctca acaattgtcg gcgcattgta tagctggcga accaggctat cgtcaaccaa
     3601 ctggcactgc tgtgcgccga gacgcatggt accgccgaga tcgctcttct cgctacgaac
     3661 ttcaacgttg ccgttttcat cgcgccactc ggtaatcagc gccacaaccg ggtacttaca
     3721 gtctggcaca aattctgtag agttggcgtt ctccatgttg gcaacatggc gagcgtaatc
     3781 aattaacgcc acctgcatac ccaggcaaat gcccagataa ggaatattgt tctcacgcgc
     3841 aaaacgcgcg gtcgtaatca tgccttctac gccacgatag ccgaaaccgc caggtacgag
     3901 gattgcgtcc agacctttaa ggatttcaac gccgcgcgtt tcaacatctt gtgaatcgat
     3961 cagtttgatg ttgacgctga cacgattctt cagcccaccg tgtttcagtg cttcgatcac
     4021 tgatttataa gcatccggca gttcaatgta cttgccgacc ataccgatgg tgacttcact
     4081 taccggattc gcttcttcga agataacctg ttcccattcg gacagatttg cttccgggca
     4141 gtttaagctg aatcgtttac aaatataatc gtccagcccc tgagatttca acaggcccgg
     4201 aattttatag atggaatcga cgtctttcag agaaataacc gctttttccg gaacattaca
     4261 gaacaatgca atcttcgcac gttcgttcgc cggtacagcg cgatctgaac gacaaatcag
     4321 gatgtcaggc tggataccga tggagagcag ctcttttaca gagtgctgag tcggtttggt
     4381 tttgacttca ccagacgctg ccatgtacgg caccagcgtc aggtgcataa acagagtgtg
     4441 ctcacggcca atttcaacag ccatctggcg aatagcttcg aggaacggca aggattcgat
     4501 atcacctact gtaccgccga tttctaccag tactacgtca tgaccttcgc caccttccag
     4561 cacgcgctct ttgattgcgt tagtgatgtg cggaataacc tgcacggttg cgccgaggta
     4621 gtcaccgcgg cgttctttac gcagaacgtc agagtagata cgacccgtgg tgaagttgtt
     4681 gcggcggctc attttggtac gaatgaaacg ctcgtagtgc cccaggtcca agtcggtttc
     4741 agcgccgtct tcagtaacga acacttcccc gtgttggatt gggctcatag tacctggatc
     4801 gacgttgatg taaggatcca gtttcatgat ggtcacattg aggccacggg cttcaagaat
     4861 ggctgcgagg gaggctgcgg caatgccttt acccagagag gatacgaccc cgccggtcac
     4921 aaaaatatag ttcgttgtca tgctgaacct gagaagttag gttgaaaaga cgatggaata
     4981 accaagacgg gaaagcagta tacccgaaca tgacctgtgc cacaaacttt cattatccct
     5041 cctcttcgcc agcgcactat tgaaatcagg agtgagaaaa tagccccttt ggggtaaatg
     5101 tttttgacgc aaatcaagcg cttgtcattt aaaaaatgac acaaatggcg cttgaccgcg
     5161 taattccctt agagatcaat ttcctgccgt tttacctgtt gccagacttc ttccattgtt
     5221 tcgaggtcaa cacctgtcat ttccagtcca cgcgcggcaa caatacgctc cacttcgcga
     5281 aaacgacgct cgaatttttc gttcactttt tgcaatgcga tttctgcttt cgtccctaaa
     5341 tggcgagcca gattaaccgt ggcaaacagc aggtccccca tttcctcctc cagtttagcc
     5401 tggtcgacaa cagcctgccg cgcctcgtac atcacctcgt cgatctcttc gtagacttta
     5461 tcaaccaccg ggccaagcgt cgtccagtcg aagccaacgt tggcgcaacg tttctggatt
     5521 ttttgcgccc gcattaaagc cggtaaacta cgaggaatat cgtccagcgc cgaatgctgc
     5581 gctttctgcg cgcgctcttc ggttttgatt tgctcccaac gggcaagcac ttcactactg
     5641 ttttcggcag aactatcagc aaaaacatgc ggatggcgac gctctaattt atcgctaata
     5701 gcagcgcaaa tatcattaaa gtcaaagcgc ccttcttcct gagccatttg cgcgtaaaac
     5761 accacctgga atagcagatc gcccagttca ccgcgaagat cgtcaaaatc ttcacgggcg
     5821 atggcgtcca gcacttcgta ggtttcttca agggtgtaag gcgcaatggt ggcaaatgtc
     5881 tgctctttat cccacgggca gccgttttcc ggatcgcgca ggcgctgcat aatagtgagc
     5941 aaacggtcga tttgattcat tgaattgtcc tgaaaattgc gggtctgtca ggtgtaatac
     6001 cttcgagtaa aagatatcgc tggatacggc tgacgccata tccagctatg taagactaac
     6061 tcccgtacaa ccgacgcgcg tcgataacat ccggcacctg gttgagttta cccagcacgc
     6121 gccccagcac ttgcaggttg taaatctcaa tggtcatgtc gatggtcgcc agttgctgtt
     6181 tggtgtcgct acggctggca acgccaagca cgttcacctt ctcgttggcg agaatggtcg
     6241 tgatatcacg taacaaccca ctacgatcat tagccaccac gcggaccacc agcgaatatc
     6301 cggcggagta gctctcaccc cataccgcgt caacaatgcg ttctggcgca tgggagcgca
     6361 gttccgccag ttgttcgcaa tcggcgcggt gtactgaaat accgcgtccc tgggtaatga
     6421 agccgacaat ctcatctcca ggaatcggct ggcagcagcg cgcgatgtgg tgcatcaggt
     6481 taccaacacc ttcaaccacc acacgaccgt tatctttact gcggttttgc ggtgtgtagc
     6541 ttttttgctg aagctgtttc agcgcggcgg cgtcctgctc ttcggcactc ggcttattaa
     6601 attgcgattg caggaagttc accatctgat tgagacggat atccccgcca ccaatcgccg
     6661 ccagcaactc gtcgacatca ttgaagttgt aacgcggcag cagatgtttt tctgcttctt
     6721 tcaggctgat ccccagatgt tccagctcgt cgtcaaggat ttgccgccca gccagaatgt
     6781 ttttgtcacg gtcctgttta cggaaccagg cgtgaatttt cgaacgccca cggctggttg
     6841 tgacgtaacc gaggtttggg tttaaccagt cacggctggg gttcggctgt ttctgggtga
     6901 taatttcaat ctggtcgccc atttgcagct ggtaggtgaa cggcacaatg cgcccgccaa
     6961 tttttgcccc gatgcagcgg tgtccgacat cactgtggat gtggtaagcg aagtccagcg
     7021 gcgttgatcc cgcaggcaaa tcaacgacat cacctttcgg cgtaaagaca tacacccggt
     7081 cgtcaaacac ctgactacgt acttcgtcga gcatttcgcc ggaatcagcc atctcttcct
     7141 gccacgcaat cagtttacgc agccaggcaa tccggtcttc atgtcccgaa cgtgcgccgc
     7201 cagctgccgc gccctcttta tatttccagt gcgcagcaac acccaactcc gcatcttcat
     7261 gcatctgttt ggtgcggatt tggatctcaa cagtttttcc acccggcccc agaaccacag
     7321 tatgaataga ctgataaccg tttggtttcg ggttagcgac gtaatcgtca aactcatccg
     7381 gcaggtggcg atagtgagtg tgcactatcc ccagtgcggc ataacaatcc tgtaaacgct
     7441 cggcgacaat acgtaccgca cgcacatcaa acagctcatc gaaggcgagg ttctttttct
     7501 gcattttgcg ccagatgctg tagatgtgtt tcggacgacc atacacttca gctttaacgc
     7561 cttcagcttt catctcagcg cgcagatggc cgacaaactc ttcgatatag tgttcgcggt
     7621 cgagacggcg ttcatgcagc agttttgcga tgcgtttgta ttcggtcggg tggagataac
     7681 ggaagcagta atcttccagc tcccatttca gttgcccaat cccaagacgg ttcgccaacg
     7741 gcgcgtagat attggtgcac tcttttgccg ccagtacacg ttcatcttcc ggcgcatctt
     7801 ttacttcgcg cagatgagca atacgctccg ccagtttgat gactacgcag cgaaaatcat
     7861 cgaccatcgc caataacatc cggcgaacgt tatcgacctg ttcggaggaa acagaatcag
     7921 tgtgcgtcgc tttcagctgg cggatcgccg ccatatcacg cacgccgtga ataaggttaa
     7981 caaccgactt accgacgctc tcacgcagca catcttcgct gactacgttg gcatcagcca
     8041 gagggaacag cagcgccgcc cgcagcgtgt caatgtccat acttaatgtc gagaggatct
     8101 ccaccatctc aacaccacgc cacaataaca gactggcatc cggatgcccc tgcgtctgtt
     8161 gcagacaata cgcccaggtt tcggctaagc actcacacga cttctggctg gtaataccca
     8221 gacttgcgat ccatttttcc ggatcaaatt caccagcctt attgatatgt gcacttctta
     8281 ccgcaaccat cgtcctctcc tttagggacc agacctgccg aaatcggcaa atcgtaacta
     8341 tttaacgcgc gagaaaagta ccatcgattc cagatgtccc gtgtgtggga acatatccag
     8401 catcgccagt cgcgcaatgg tatatcctgc ttttaataac gcttcgctat cccgagccag
     8461 cgttgcaggg ttacaggata cataaactat acgaataggt tccagtttta taatttgctg
     8521 cataacacct gcggcacctg ctcgcgccgg gtccagcaac actttatcga agccgttttt
     8581 cgcccacggc tgctttgtga catcttcttc aagattttcg tgataaaacg tcacattctg
     8641 taagccatta agacgcgcat tctgctggcc tttttccacc agcgccggaa caccttctac
     8701 accgaccaca ctggcagctt gtgtcgccaa tggcagtgta aagttgccca taccgcagaa
     8761 cagatccagt acgcgatctt caggttgcac atccagccat tccaacgcac gcgctaccat
     8821 tttttggttc acacccgcat tgacctgaat aaaatcgcgc gggctaaaag ttaagcgcaa
     8881 cccgtttgag tcataccagg gcatctcacc agagacggtt tcgagtatct cactatcggg
     8941 ggcgagatac agatccaggc cttcagaatg cgaaaagcgt tccagttttt cgcgatctgc
     9001 cgaacttagc ggtgcggtat ggcgcaaaat catcagcgtg ccgctggttg cctgtaccag
     9061 ttcaacatga ccaagatggc gcatagcttg taagctgccc agacatgccc tgactttggg
     9121 cagcaatgct tcaagttggg gcgctaaaat ggggcattgt ttgacgtcga caatgtcact
     9181 ggagcccgct ttgcgaaacc ccatctgaag ttgctgtgtt ttcggtaagt agttcagact
     9241 taaacgcgcg cggcggcgat agccccaggg aacatcggcg atcacttcag agacatcgtg
     9301 tttcattaat cgggcgagtg ccgcactttt gcttcgctgc tgtaaatcca cgctggcgtg
     9361 ttgttgctga cagccgccgc atacgccaaa atgaggacag cgtggcgttt cgcgttccgg
     9421 gctatcgctt aaccggcgta cgactttagc gcgggcatac tgttttttat cttcagtaac
     9481 agcaacttcc gcgttttcct gcggcaataa tccggggata aataacgttt tgccgttatg
     9541 tcgcgccacg ccctgaccaa aagagtcgag gtcgttgact gaaacggtta tgatctgacg
     9601 cgtcgtcgtg cgtcgttttg cagagtagaa ttgcgccatt ggcgagactt tctcaattta
     9661 acagtgtgac cttaattgtc ccataacgga actccatgac caactacagc ctgcgcgcac
     9721 gcatgatgat tctgatcctg gcaccgaccg tccttattgg tttattgctg agtatctttt
     9781 tcgtcgtgca tcgctataac gacttgcagc gtcaactgga agatgccggt gctagcatta
     9841 ttgagccgct tgcagtttct actgaatatg gcatgagcct gcaaaatcgc gaatctatcg
     9901 gtcagttaat aagcgtactg catcgtcgcc attccgatat tgttcgcgcg atttcggttt
     9961 atgatgaaaa taaccgactc tttgtcacct ccaattttca tcttgatccc tcatcaatgc
    10021 agctcggcag caacgtgccg tttcctcgcc agctcactgt cactcgtgac ggcgatatta
    10081 tgatcctccg cacgccgatt atttctgaga gttactcccc cgacgaatcg cccagtagcg
    10141 atgccaaaaa tagtcaaaat atgttgggat atattgcgct tgagctggat cttaaatcgg
    10201 ttcgcttgca gcaatataaa gagatcttta tttcctgcgt gatgatgctg ttttgtatcg
    10261 gtattgcgct tatttttggc tggcgcttaa tgcgcgatgt aaccggtccg attcgcaaca
    10321 tggtgaatac cgtcgaccgc atccgtcgcg ggcaactcga cagccgggtg gaaggattta
    10381 tgctcggcga gctggatatg ctgaaaaacg gtatcaactc gatggcaatg tcgctggctg
    10441 cttatcacga agagatgcag cacaatatcg accaggcgac gtccgatctg cgtgaaacgc
    10501 tggagcagat ggaaattcag aacgttgagt tggatctggc gaaaaagcgc gcccaggaag
    10561 cggcgcgtat taaatccgag tttctggcaa atatgtcaca cgagctgcgt acaccactga
    10621 atggtgttat tggctttacc cgcctgacgc tgaaaacaga attaacacca acgcagcgcg
    10681 atcacctgaa tacgattgaa cgttcggcaa ataatttgct ggcaattatt aatgatgttc
    10741 tcgacttctc gaaactggaa gcaggtaagc tgattctgga aagtattcca ttcccactac
    10801 gcagcacgct ggatgaagtc gttactctgc tggcacattc ttctcacgat aaagggttgg
    10861 aactgacgct taatattaaa agcgacgtgc ctgataacgt gatcggcgac ccactgcgat
    10921 tacagcaaat catcactaac ctagtgggga atgcaattaa attcaccgag aatggcaaca
    10981 ttgatattct ggtagaaaaa cgtgcgctga gtaataccaa agtgcagatt gaagtgcaga
    11041 ttcgggatac cggcattggt attcctgaac gcgatcaatc gcgcttattc caggccttcc
    11101 gacaggctga tgccagtatt tcccgccgtc atggtggcac cgggctgggg ctggtgatta
    11161 cacaaaaact ggttaatgaa atgggcggcg atatttcgtt ccatagccag ccgaatcgcg
    11221 gttcaacttt ctggttccac attaatctcg atctgaaccc gaacattatt atcgaagggc
    11281 catccatcca gtgcctcgca ggtaagcgcc tggcctatgt cgaaccaaac tccgcagcag
    11341 cgcaatgcac gctggatatt ttaagtgaaa cgccgctgga agtggtttat agcccaacgt
    11401 tctccgcgct gcctcccgcg cattacgaca tgatgttgtt aggcatcgcg gtgaccttcc
    11461 gcgagccgct aacaatgcaa catgagcgat tagcgaaagc ggtatcgatg accgatttcc
    11521 tgatgctggc acttccttgc catgcacaag tcaatgctga aaaactcaag caagatggta
    11581 tcggcgcgtg tctgctgaaa ccattaacac ctacgcgcct gttgcctgcc ctgacggaat
    11641 tttgtcatca caaacaaaac acgcttttgc ctgtaaccga tgaaagtaag ctggcaatga
    11701 cagtcatggc ggttgatgac aaccccgcta acctgaaact tatcggcgca ttgctggaag
    11761 atatggtgca acatgtggaa ctttgcgata gcgggcatca ggcagttgaa cgggcgaaac
    11821 agatgccgtt cgatttgatc ttaatggata ttcaaatgcc ggacatggat ggcattcggg
    11881 cctgcgaact catccaccaa ctcccgcatc agcgacaaac gccggttatc gcggtaacgg
    11941 cgcatgcaat ggccgggcaa aaagagaagc tgcttggcgc agggatgagc gattatctgg
    12001 cgaaaccgat tgaagaagag cgattgcata atttgttgtt gcgctacaag cctggcagcg
    12061 gtatttcgtc tcgcgtcgtg acgcccgaag tcaacgaaat tgtggtgaac ccgaatgcga
    12121 ccctcgactg gcaactggca ctacgccagg cagcaggaaa aaccgattta gcgcgcgata
    12181 tgctgcaaat gttactcgat ttcctgcctg aagttcgcaa caaagttgag gaacagctgg
    12241 ttggagaaaa cccggaaggc ctggttgatt tgattcataa actgcatggc agttgcggct
    12301 atagcggcgt accacgtatg aagaatctct gccagctgat cgaacaacag ctacgtagcg
    12361 gtactaaaga agaagatttg gagccggagc tgctggaact gttggacgag atggataatg
    12421 tcgcgcgcga agccagcaaa attctcgggt aacggacgtc ttgtcgagta gcaatcggct
    12481 ttttatgacg ctggtttcag attggtgaca aagtgcctgt gtttatgccg gatgcggcgt
    12541 taacgcctta tccggcctac aaaaccaatt aaattcaatg aattaaaaaa tgatgtagac
    12601 ctgatacata gcacaacagg ctattttgcg tttagcatca gtctcaaacc ggctccagat
    12661 agagccggtt ttggttttct gtcttaacgc accatgcacg ggcgcttgtt atcgaacgtc
    12721 cagccaggaa tcagatactg cattcccatc gcatcgtcac gcgcgccaag cccgtgtttc
    12781 tgatacagct catgggcttt catcacttga tccatatcga tttctacacc cagccccggt
    12841 ttttctggca cctgtaccag cccgcctttg atctcaaacg gttctttggt caggcgctga
    12901 ttaccttcct gccagatcca gtgcgtatca atagcagtaa ttttacccgg tgcagcggcg
    12961 gcaacatggg taaacatcgc cagggaaata tcgaagtggt tgtttgagtg tgaaccccag
    13021 gtcaggccaa attcatggca catttgcgcc acacgtaccg aaccttgcat agtccagaaa
    13081 tgcggatccg ccagcgggat atcaacggat tgtaacgaga gcgtatggcc catctgccgc
    13141 cagtcggtgg cgatcatatt ggttgcagtc ggcaggccag tcgcgcgacg gaactctgcc
    13201 atcacttcac gcccggagaa accttgctcc gcaccacacg gatcttctgc ataagccagc
    13261 gaacctttca ggtatttacc aattttaatt gcttcgttca gcgaccaggc accgttagga
    13321 tcgagcgtaa tacgcgcctg cgggaagcgt tgcgccagtg ccacaataga ctcggcttct
    13381 tcttccccgg ccagtacacc gcctttcagt ttgaaatcgt tgaagccata tttttcatac
    13441 gccgcttccg ccaggcgcac caccgcatcc ggcgtcatcg cttcttcatg acgcaggcga
    13501 taccagtcgc atgagtcatc cggctggctt tgatacggca gaggtgtggc tttgcgatta
    13561 ccgacgaaga acagataacc gagcatttcg acttcgctac gctgttgacc atcgcccagc
    13621 agcgatgcca cgtttacccc cagatgctgc cccagcagat ccagcattgc cgcttctatc
    13681 ccggtaacta catgaatagt ggtacgcaaa tcaaatgtct gcaaaccgcg cccactagca
    13741 tcacgatcgg caaaagtatt acgcaccagc gtcagaacgt ttttgtattc acccagcgtt
    13801 ttacctacca ccagcggaat cgcatcttcc agcgttttac ggattttctc gccgccggga
    13861 atttccccta cgccagtgtg accggaatta tctttgataa tcacaatatt acgcgtgaag
    13921 aacggtgcgt gtgcaccact cagattcatc agcatactgt catgacccgc caccgggatg
    13981 acctgcattt cagtaacaac aggcgtcgta aattgagaac tcatatttat gtcctttttc
    14041 agaatcaatg acggccgaaa acgggacgtt tacggtcaaa ggtccagccg gggatcaggt
    14101 actgcatcgg acctgcgtcg ttacgcgcac cgcaaggcag acgtttatag gcctcatgtg
    14161 ccttttgtac ctgttcccag tccagttcca cgcccagacc gggcgcatca ggaacggcaa
    14221 tttttccgtt tttaatctcc agcggatttt tggtcaggcg acaatcgccc tcctgccaaa
    14281 tccagtgggt atcgatagcg gtaggattac ccggtgccgc cgcgcccaca tgggtaaata
    14341 tcgccagaga gatatcgaaa tggttgttgg aatggcagcc ccaggtcagc ccccagtcgt
    14401 cgcaaagctg cgccacgcgg actgcaccgg aaagcgtcca gaagtgcgga tcggcaagtg
    14461 gaatatctac cgcattgagc atcaccgcat gacccatttc gcgccagttg gtggcgatca
    14521 tgttagtcgc gacgggcaag ccggtcgccc gtcgaaactc cgccatcact tcacgtccgg
    14581 agaagccctg ttctgcgccg catggatctt cggcataggt aagaacatca ttcagccctt
    14641 tgcataaaga aatggcttca tcaagcagcc atgcaccgtt gggatcaacg gtaatccgcg
    14701 catccgggaa gcttttcttc aatgcacgaa cagtgtcgat ttcttgctcg ccaggtaaca
    14761 ccccgccctt aagtttgaaa tctttaaagc catagcgatc ctgtgaggct tccgccagac
    14821 gcacaacggc ttcgctgttc atcgctttct gatggcgcaa ctgataccac tcatggttgc
    14881 ccggcgtatt ttccagataa ggaagatcgg ttttggtccg atcacctata taaaacagat
    14941 aaccgaggac ggtaatagtc tcgcgttgct tgcctggccc caacagttcg cagaccggaa
    15001 cattcagcgc cttacctagc aggtcaagca aggcggcttc cagcgccgcc acggcattaa
    15061 cgcgcaattc aaaagtccag gcacctttgc cgaaggtctc aaaatcggct gcctgatttc
    15121 ctttatgtac ctgctgaacc actttattca ggcgcgcaac ttcctggccc agaaccatcg
    15181 gaatagcatc gaccagtgtt tgataaatca cctctccgcc cggcgcttca ccaatgccgg
    15241 tatgcccggc gttatcggtg agtaccacga tattgcgggt gaagtatgcg ttatgcgcac
    15301 caccaatatt gagcaacatg ctgtcatgtc cggcgaccgg aatgactttc atatcagtaa
    15361 taacaggact ggattgtgtc gccatcttat tgccccgcga caggtttcag ctcgatacgc
    15421 ttgatatcgc ccaccagcac caggtagctc agtaccgcga ttagggcatg aacaccaaca
    15481 taaatcagcg ccccattaaa cgagccagtc gtgccaacga tataaccaat tgcgattggc
    15541 gtgacgatgc cagaaatgtt accgaacatg ttgaactggc caccggaaag accgctgatc
    15601 tcttttggcg cggtatctgc cattactgcc caacccagcg ccccgatgcc cttaccgaag
    15661 aaggccagcg ccataaagcc gatgatcatc cactcaacgt tgacgtagtt gcagaacacc
    15721 atcaccatcg acagcaacat gcccattacg atcggtgttt tacgcgcaat gttcagcgat
    15781 cccgtgcggc gcatcagcca gtcggaaata attccaccca gcacaccgcc gataaaaccg
    15841 caaaccgccg gaacggaagc cacaaagccc gctttcagaa tcgacatccc acgcgcctgc
    15901 accagataaa ccggaaacca ggtaatgaaa aagtaagtca gggcgttgat acagtactga
    15961 ccgatataaa cgccgatcat catccgtgac ccgagcagct gtttgatctg cccccacttc
    16021 acgctgaacg gcactttaac tttggtgctt tgctgatcca tattgatcag cgcgccaccc
    16081 gcggcgatat actccagctc tttctgattt acccccggat gttgatttgg ctcgtggatg
    16141 actttcaacc agataaagct gatgacaatg cccagaccgc ccataaagaa gaagacgtgt
    16201 gaccagccca cttcatgcgt cagccagccc ataatcggcg cgaagatcac cgttgcgaag
    16261 tattgagcgg agttaaaaat cgacaccgcc gttccccttt cctgcgccgg aaaccaggcc
    16321 gcaacaatgc gactgttgcc ggggaaagat ggcgcttcag caagcccgac caggaagcgc
    16381 agagtaaaca gggcaacgat aatgccgaat ccactaaaga tatcgacgaa gccttgcagc
    16441 aaggtaaaca tcgaccagat aaagatcgac cagaagtaga cgcgttttga accaaaacga
    16501 tccagcaacc aaccaccagg gatctgcccg ataacataag cccatgagaa ggcagagaac
    16561 acatagccca ttcccacggg atcaaggccg atatctttgg ccatttccga accggcaata
    16621 gaaagcgtag cgcggtcgcc gtagttgaag gatgtgacga taaacaacat caccactatc
    16681 cagtaacgag cattcgtgcg tttttccaca ctgctcgcag cctgacttaa agaactcatt
    16741 gttgcactcc tgaaaattcg cgttagccac gctcactctg gactgcgaca tcgccaggaa
    16801 atcagaggtg acgtagggtg ttttttgccg tttttatagg tcgttcgccg aatacggcgc
    16861 gtgtttatat ctggcaatag cagtataaaa agcgcgccat agcggctcac cgtgcaacaa
    16921 cacaacatta atgcgttcaa tgaggcccga ttttggcatt agccctggac ggtggaatcc
    16981 acttcacgga aatgaaaaca agaacaagaa aggaagggta aaaacgaaga aataaagaag
    17041 agtatgaaat ggatcgcttg actctaggca aacgccagta aaaatccgcg ctatgaagca
    17101 gtttttactg gcatttgcct gaaaagattc gattcagcac cgctaaaacg acatttaccg
    17161 ctcgctgaca tatcaggaca acagcgtgcc ccactgttcg acccacggat ttgattccgt
    17221 ttccggttcc gggttctcac tggcgtcaat caacagcatt tcgccaaccc gctgagcgct
    17281 ctgttcctgc aacaaggcat cgaactgttt gccgccattg caaaaattta cataactgct
    17341 gtcgccgagc gcaatcacgc cataacgcag attcggctgg aagcccagac tatctttgat
    17401 tccctgaaag agcggcacaa tgctatcagg aaggtcgccc tgcccggtcg tggacgtaac
    17461 caccagaaca tacttatcct gatagggcag ccagtcgctt aattcaggat cttcaaatac
    17521 cgttgctttg tggccctgtg cggtcagaat tgcttctgct tcttccgcca ctaacagcga
    17581 atttccgtac atggtgccga caaaaatacc aatttccgcc ataccgtttt ctccctggat
    17641 taggaactta tctctccatc ctgacccgat ggcgcactga actcaaccct ttcattttca
    17701 ggaagcagac cgcgccagcc aaattgtgat aacgcctgca tccaggtgtc gtccaggccc
    17761 gcgtgaatag tcagcggctc gccagtaaaa ggatgcgtca atgacaactg actggcatgt
    17821 agcattaacc gctggaggcc aaaatgctca gcaccgctgc gattctggcg taaatctcca
    17881 tgtttgctat cgccaataat cggatgacgc aaatgggcaa gatgtcggcg aagctgatgt
    17941 ttgcgtccgg ttttcggctc cagttccacc aggccgtagc gcgtggtcgg gtaacgtccg
    18001 gtcgctaccg gcatttctac ggtcgccaga ccgcgataat gcgtcactgc tggctgcggg
    18061 cctttatctt cgcgggcaaa tttatcagcg attttgtcca gttcttccac cagtggataa
    18121 tccaacaccg cttcttccat caaccagccg cgcacaatcg catggtaacg tttctggatt
    18181 tggtgctgtt caaactgttg tgccagcagc cgtccggcct cgctggataa tcccatcaac
    18241 aacacaccag aagtgggtcg gtccagacga tgaacagtaa aaacatgctg tcctatctgg
    18301 tcacgcacgg tttgcatgac cactactttc tcgtcgcgat ccagccagct acggtgaacc
    18361 agccagccgg agggtttatt taccgcaacc agccattcat cctgatagag tatttccagc
    18421 attagctcgc atcatccgca aaaagagcat ccagtttttc caactcagcc agaataagcg
    18481 cgcgttgcgg atggtccgtc gctagcgcca tttcataata gggtgcaaca gcaaaagcgc
    18541 ccggtaacgg ctggttgtta tttaacaaat cgtgcattcg cgggatcagc ccccactgca
    18601 accactccag tggttccatg gtgtccataa agaacggttg ggtactatta aattgatgcg
    18661 gctggggttc atcgtttcgc cagtgctgat gttcacgcag taacgcttca agcgcctgca
    18721 actggagacg aacgcggtca tgagtggtca taaagcaacc tcaataagaa aaactgccgc
    18781 gaaggatagc aggaataaaa aaagggagca ctgtattcac agtgctcccg gttcgtttcg
    18841 cagcattcca gctacttttg ttgctccctg ctcatccttg acaacttttc ctctggcctt
    18901 gcggccaatc gttcatcctg aactattgct tcctgctcac accaccccga tgtgatactt
    18961 catcctgaag tgtccctggc cttcctgacc caccgaatca tcctgaccgg ttctcattct
    19021 ccatcctgga ggtgtccttt aacgcgtcct gcgtcatcct cttcgcttca tccagaagcc
    19081 tttccctgaa acaccatcct ggtgtgtcct gcagaagtgt catcatcctg atgttcactt
    19141 cgttgtctga ctccctgtcg acgaagatag aatcgtcttt ttcgcgaagt cttacaaggc
    19201 gcttacagac aatgccttaa ggaaaatttc atatgaaagt taagaattaa caatcaacat
    19261 tttgatttta aatgaaaata acgattctta tctttttaag tattcgctta tttcctggcg
    19321 atctcctaca gagtctgtaa gagatctctc acaaccagat ggggtatttg gattacagaa
    19381 gaggcttaag ttgattaagg aattccgcaa gatttgaggc taaatgcatc cgtttacgtg
    19441 ttcccagcgt ctctttgcac acttcaccgg agagattaca caccgagatg acctctaact
    19501 cttcttccag cgtcgcgata aatagcgtgg gcggcagttt tagccttttc tgagtcacca
    19561 gatgaccaat cagattttcc tgcacccgac ggaagtcgtc ttcactccag gtttgcagca
    19621 atgtcagttt gatatcgcca aactgcgcgt gcatatcccc ggcaaactga gtcgtataaa
    19681 aggtatgaat tgtgggttgt atcacaatat caaaagcgcg ttcaaccgcg tttacatttt
    19741 gttccccggt aaacggctgg ggttgccagt atacggcatc ttcggtagtt gaaataatgc
    19801 acggtgaagg aacaccgtac agttcctcac ttaacggcca gcttttatgc tcttcatgcc
    19861 acgcatcgca gtagcgtgca gtaaaatctt tcagggcttg tgcggtcaaa tcgtccacta
    19921 atttctctct tcacttaaac cagatacact tgtcgcttag tttatctggt ttatgacggt
    19981 gaaacatgtc ttcttatgca aaccatcagg cacttgcggg cctgactctt ggaaaatcaa
    20041 ccgattaccg ggatacctat gacgccagcc tactgcaagg cgttccacgc agcctgaatc
    20101 gcgacccgct gggtctgaaa gcggataacc tgccttttca cggtacggat atctggacgc
    20161 tgtatgaact ttcctggctg aatgcgaaag gtttgccgca ggtcgctgtc ggtcatgttg
    20221 aacttgatta caccagcgta aatctgattg agtcgaagag ttttaagctc tatctcaaca
    20281 gttttaacca gacgcgtttt aataactggg atgaggtgcg ccagacgctg gagcgcgact
    20341 taagcacttg cgctcagggt aagattagcg tggcgttata tcgtcttgat gaactggaag
    20401 gccagccgat aggtcatttc aatggcactt gcattgatga ccaggatatc actatcgata
    20461 actatgaatt cactactgac tatctggaga atgccaccag cggtgaaaaa gtagtggaag
    20521 agacgctcgt cagccacctg ctgaaatcaa actgcctgat cacccatcaa ccagattggg
    20581 gttcgatcca aattcagtat cgtggacgcc aaattgacag agaaaaactg ctgcgttacc
    20641 tggtctcatt ccgtcatcac aacgagttcc acgaacagtg cgtggaacgc atctttaatg
    20701 acctgttacg cttctgccag ccagagaaat tgagcgttta cgcacgttat acccgtcgtg
    20761 gcggtctgga cattaacccg tggcgcagta atagcgattt tgtcccatcg accacaagac
    20821 tggttcggca ataaattttt tctcaatttt gcgtgctgga ttcacgcaga aggttgtgaa
    20881 aggtcatcag gcagggctat tgtaatcaaa gggaatgacg atattcgtcc cataaggagt
    20941 ttttcttgat tacacatatt agcccgcttg gctccatgga tatgttgtcg cagctggaag
    21001 tggatatgct taaacgcacc gccagcagcg acctctatca actgtttcgc aactgttcac
    21061 ttgccgtact gaactccggt agtttgaccg ataacagcaa agaattgctg tctcgttttg
    21121 aaaatttcga tattaacgtc ctgcgccgtg aacgcggcgt aaagctggaa ctgattaatc
    21181 ccccggaaga ggcttttgtc gatgggcgaa ttattcgcgc tttgcaggcc aacttgttcg
    21241 cggttctgcg agacattctc ttcgtttacg ggcaaatcca taataccgtt cgttttccca
    21301 acctgaatct cgacaactcc gtccacatca ctaacctggt cttttccatc ttgcgtaacg
    21361 ctcgcgcgct gcatgtgggt gaagcgccaa atatggtggt ctgctggggc ggtcactcaa
    21421 ttaacgaaaa cgagtatttg tatgcccgtc gcgtcgggaa ccagctgggc ctgcgtgagc
    21481 tgaatatctg caccggctgt ggtccaggag cgatggaagc gccgatgaaa ggtgctgcgg
    21541 tcggacacgc gcagcagcgt tacaaagaca gtcgttttat tggtatgaca gagccgtcga
    21601 ttatcgccgc tgaaccgcct aacccgctgg tcaacgaatt gatcatcatg ccggatatcg
    21661 aaaaacgtct ggaagcgttt gtccgtatcg ctcacggcat cattatcttc cctggcggtg
    21721 tgggtacggc agaagagttg ctgtatttgc tgggaatttt aatgaacccg gccaacaaag
    21781 atcaggtttt accattgatc ctcaccggcc cgaaagagag cgccgactac ttccgcgtac
    21841 tggacgagtt tgtcgtacat acgctgggcg aaaacgcgcg ccgccattac cgcatcatca
    21901 ttgatgacgc cgctgaagtc gctcgtcaga tgaaaaaatc gatgccgctg gtgaaagaaa
    21961 atcgccgtga tacaggcgat gcctacagct ttaactggtc aatgcgcatt gcgccagatt
    22021 tgcaaatgcc gtttgagccg tctcacgaga atatggctaa tctgaagctt tacccggatc
    22081 aacctgttga agtgctggct gccgacctgc gccgtgcgtt ctccggtatt gtggcgggta
    22141 acgtaaaaga agtcggtatt cgcgccattg aagagtttgg tccttacaaa atcaacggcg
    22201 ataaagagat tatgcgtcgt atggacgacc tgctacaggg ttttgttgcc cagcatcgta
    22261 tgaagttgcc aggctcagcc tacatccctt gctacgaaat ctgcacgtaa tctcccctcc
    22321 cccggtgtaa tgccgatcag ttaaggatca gttgacc
//
LOCUS       DAJVQH010000060        22185 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJVQH010000060 DAJVQH010000000
VERSION     DAJVQH010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.060"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..1971)
                     /gene="metG"
                     /locus_tag="OQM87_002324"
     CDS             complement(<1..1971)
                     /gene="metG"
                     /locus_tag="OQM87_002324"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCS1508440.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPG"
     gene            2103..3212
                     /gene="apbC"
                     /locus_tag="OQM87_002325"
     CDS             2103..3212
                     /gene="apbC"
                     /locus_tag="OQM87_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCS1508441.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3474..3755
                     /locus_tag="OQM87_002326"
     CDS             3474..3755
                     /locus_tag="OQM87_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCS1508442.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4048..4590
                     /locus_tag="OQM87_002327"
     CDS             4048..4590
                     /locus_tag="OQM87_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1508443.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            4671..5345
                     /locus_tag="OQM87_002328"
     CDS             4671..5345
                     /locus_tag="OQM87_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1508444.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5361..7841
                     /locus_tag="OQM87_002329"
     CDS             5361..7841
                     /locus_tag="OQM87_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1508445.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            7857..8890
                     /locus_tag="OQM87_002330"
                     /pseudo
     CDS             7857..8890
                     /locus_tag="OQM87_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(8972..9310)
                     /gene="rcnB"
                     /locus_tag="OQM87_002331"
     CDS             complement(8972..9310)
                     /gene="rcnB"
                     /locus_tag="OQM87_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCS1508446.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9529..10371)
                     /gene="rcnA"
                     /locus_tag="OQM87_002332"
     CDS             complement(9529..10371)
                     /gene="rcnA"
                     /locus_tag="OQM87_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCS1508447.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHEHHQDHEHHHDQGHHHHHEHGEYQDAHA
                     RAHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVS
                     FSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFM
                     GIMR"
     gene            10492..10764
                     /gene="rcnR"
                     /locus_tag="OQM87_002333"
     CDS             10492..10764
                     /gene="rcnR"
                     /locus_tag="OQM87_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCS1508448.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      10856..10952
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10987..11775
                     /gene="thiM"
                     /locus_tag="OQM87_002334"
     CDS             10987..11775
                     /gene="thiM"
                     /locus_tag="OQM87_002334"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCS1508449.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            11772..12572
                     /gene="thiD"
                     /locus_tag="OQM87_002335"
     CDS             11772..12572
                     /gene="thiD"
                     /locus_tag="OQM87_002335"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCS1508450.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            12637..13455
                     /locus_tag="OQM87_002336"
     CDS             12637..13455
                     /locus_tag="OQM87_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCS1508451.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            13507..14253
                     /locus_tag="OQM87_002337"
     CDS             13507..14253
                     /locus_tag="OQM87_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1508452.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14227..15192)
                     /locus_tag="OQM87_002338"
     CDS             complement(14227..15192)
                     /locus_tag="OQM87_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1508453.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15189..16192)
                     /locus_tag="OQM87_002339"
                     /pseudo
     CDS             complement(15189..16192)
                     /locus_tag="OQM87_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16189..16665)
                     /locus_tag="OQM87_002340"
                     /pseudo
     CDS             complement(16189..16665)
                     /locus_tag="OQM87_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            16819..17871
                     /gene="fbaB"
                     /locus_tag="OQM87_002341"
     CDS             16819..17871
                     /gene="fbaB"
                     /locus_tag="OQM87_002341"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCS1508454.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            18179..19033
                     /gene="gatY"
                     /locus_tag="OQM87_002342"
     CDS             18179..19033
                     /gene="gatY"
                     /locus_tag="OQM87_002342"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCS1508455.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            19062..20329
                     /gene="gatZ"
                     /locus_tag="OQM87_002343"
                     /pseudo
     CDS             19062..20329
                     /gene="gatZ"
                     /locus_tag="OQM87_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            20339..20791
                     /gene="gatA"
                     /locus_tag="OQM87_002344"
     CDS             20339..20791
                     /gene="gatA"
                     /locus_tag="OQM87_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS1508456.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            20822..21106
                     /gene="gatB"
                     /locus_tag="OQM87_002345"
     CDS             20822..21106
                     /gene="gatB"
                     /locus_tag="OQM87_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCS1508457.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            21110..22185
                     /locus_tag="OQM87_002346"
                     /pseudo
     CDS             21110..22185
                     /locus_tag="OQM87_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
BASE COUNT         5764 a         5141 c         5624 g         5656 t
ORIGIN      
        1 gccaggcccg gcagccatca ccatgccttc agagataccg aagcgcattt tacgtggtgc
       61 caggttagcc accataatgg tgtgacgacc aatcagtgcc tgcggatccg ggtaagcaga
      121 acgaataccg gagaagacat tgcgtttttc accgccgaga tccagcgtca ggcgcagcag
      181 tttgtcagaa ccttcaacaa actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt
      241 ggcgaagtcg tcaaaggtga tggtttcctg aatcggatca tctgccagtg ggccagtaac
      301 tggcgcggta gtggctttca cttcttcttt agaggcttcc accagagctt caacctgttt
      361 catatcgatg cggttataca gcgccttgaa cggattcact ttgtggccca gcagcggttg
      421 ctggatacca tcccaggtca gttccgtatt gaggaaggct tctgcacgct cggtcagttt
      481 cggcagtacc ggcttcaggt aagtcatcag cacgcggaac aggttgatgc ccattgagca
      541 aatcgcctgc aggtcagcat cgcgaccttc ctgttttgcg actacccacg gagcctgctc
      601 gtcgacatag cggttagcca ggtcagccag tgccatgatt tcgcgcactg ctttaccaaa
      661 ttcacggctt tcccacgctt caccaatcac ttcagcggca tcggtaaagg ttttgtacag
      721 ctcagggtca gccagctcgc ttgccagcac accgtcaaaa cgcttgttga taaagcccgc
      781 attacgggag gccaggttaa ccactttgtt cacgatatcg gcattcacac gctgaacgaa
      841 atcttccagg ttgagatcga tatcatcaat gcgcgaagag agtttcgcag tgtagtagta
      901 acgcaggctg tcagcgtcaa aatgattcag ccaggtgctg gctttaataa aggtgccgcg
      961 agacttggac atcttcgcgc cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg
     1021 cttgcggaag ttgctgcctt ccagcatggc aggccagaac aggctgtgga agtaaacgat
     1081 atctttaccg atgaagtggt acagctcggc ggtggagtct ttcttccagt attcatcgaa
     1141 gcttacgctg tcgccgcgct tgtcgcacag attcttgaaa gaacccatgt agccaatcgg
     1201 tgcgtccagc cagacgtaga aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta
     1261 tggcgcgtcg cgggagatat cccactgttg caggccggat tcaaaccact cctgcatttt
     1321 gtttgccacc tgctcctgca acgcaccgct gcgggtccat gcctgcagca tttcgctgaa
     1381 agagggcaga tcaaagaaga agtgttcaga atcacgcatc actggtgtag caccagaaac
     1441 caccgatttc ggctcgatca attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt
     1501 atcgccgtat tgatccgggg atttacattt cgggcaggtg cctttcacaa aacggtccgg
     1561 caggaacatg cctttttccg gatcgtacag ctgagagatg gtgcggtttt taataaaacc
     1621 gttttctttc aggcgagagt agataagttc tgacaactgg cggttctctt cgctgtgcgt
     1681 cgagtgatag ttgtcatagc tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact
     1741 catttcgcca atcatctgct ccggggtgat accgagctgc tgagctttca gcatgatcgg
     1801 tgtaccgtgg gcatcgtcgg cgcagatgaa gttgacctcg tggccgcgca ttcgctggta
     1861 acggacccag acatcagcct ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt
     1921 agcgtacggc agtgcgcacg tcaccagaat tttcttcgcg acttgagtca tagtaggcat
     1981 tacttcttaa tagtgaaaag gggttagata gtaccaaatg ggaaaatgtt aagtaagtac
     2041 ttctctatat tcttcaggta aactatcagc tgatggttta tctatacaaa aaaggagtcg
     2101 ggatgaacga acaatcccag gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga
     2161 cactggccaa ttttcagcac ccaaccctga agcataacct caccacgctg aaagcgttgc
     2221 accatgtcgc ctggatggac gacacgttgc atgtggaact ggttatgccg tttgtctggc
     2281 atagcgcgtt cgaagagtta aaagagcaat gtagcgccga actgctgcgc atcaccgggg
     2341 cgaaagctat cgactggaaa ctgtcgcata acatagccac tctgaaacgc gtgaagaatc
     2401 agccgggaat taacggtgtg aagaacatta tcgcggtgag ttccggtaaa ggcggcgtgg
     2461 gtaaatcgtc cacggcggta aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg
     2521 gcattctgga cgccgatatc tacggcccat caattcccac catgctgggc gcagaaaatc
     2581 agcgtccaac ttcgccggac ggtacccaca tggcaccgat tatgtctcat ggcctggcaa
     2641 ccaattcaat tggttatctg gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg
     2701 cgagcaaggc gttaatgcag atgttgcagg aaaccttgtg gccggatctc gactatctgg
     2761 tgcttgatat gccgccaggc accggtgaca ttcaattgac gctggcgcaa aacattccag
     2821 taactggtgc agtcgtggtc accaccccgc aggacatcgc gttgatcgat gcgaaaaaag
     2881 gcattgtgat gttcgagaaa gtcgaggttc cggtgctcgg tattgtcgag aacatgagtg
     2941 tgcatatttg cagtaactgt ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga
     3001 aactggctga aaaataccat acacagctgc tggggcagat gccgttacac atctccttac
     3061 gtgaggatct cgataaaggc acgccaacgg ttatcagccg tccggagagc gaatttaccg
     3121 ctatctaccg ccagctggct gaccgcgttg cagctcagct ctactggcag ggtgaagtca
     3181 ttcctggcga gatttccttc cgcgcggtct aacgcctatt aaccattctg gttattcttc
     3241 atacatcgca gagatgttat attgatgcgg tgtatttgtt tttgccgcat taatgaacac
     3301 ttatttaata ctgcgaatat tctgcggacc ttcgtccgaa actctacaaa gtcgagtgat
     3361 cgtacaggca atgcttcatt atcatccttc gtgctgtgta gtggatgtac agctatagtt
     3421 tgttatgcac tcattctatc caggcataac ctttactaca gggataacgt tcaatgaata
     3481 agtattggct gtcaggaatt atttttcttg cctatggatt ggcttctccc gcagtctctt
     3541 cagagactgc cacattaacg attaatggca ggatcagtcc cccaacatgc agtatggcta
     3601 tggttaatag tcaaccacaa cagcattgtg gtcagttaac gtataatgtt gacacccgtc
     3661 atcaggtttc ttcgcccgtc aaaggtgtta cgacagaagt ggttgttgca ggtagcgata
     3721 gcaaacgtcg aatcgtgtta aatcgctacg attaatttgg caacctgatt ttaattgcat
     3781 ttagaaagac gaaatcattt atataaatga tttcgtcttt tttattgata tcaacaatac
     3841 catttacata aatctacatt aattaataga tgtgataaat atttaatatt cttaagtatt
     3901 tgttgtattt atattgcatt ggttaatttg ttttaaatca ataaaacaat atgtttacat
     3961 tgcggcagct tttttgatgt gagaaatttg tttccaagag atacagactc ttaacaacca
     4021 aaaataaatt ttattaagga acatattatg aaacgttcaa ttattgctgc cgctgtcttt
     4081 tcttcttttt ttatgagcgc tggagtattt gctgcagacg ttgataccgg aacattaacc
     4141 attaaaggaa atattgcaga atctccgtgt aaattcgaag cgggtggtga ttcagtaagt
     4201 attaatatgc cgactgtacc aaccactgtc tttgaaggta aagctaaata ttctacctat
     4261 gatgatgcag tcggtgtaac cagcagcatg ttaaaaatta gctgcccgaa agaagttgct
     4321 ggtgtaaaac tctcgttgat cacaaacgac aaaataaccg gtaacgataa ggcgatagcc
     4381 agtagcaacg ataccgtggg ttactatctc tatttaggtg ataacagcga tgtcctagat
     4441 gtttctgcac cttttaacat tgagagttat aaaacagcag aaggtcaata tgctattccg
     4501 tttaaagcaa aatacctgaa actgacagat aactcagtgc aatcaggtga tgtgttatct
     4561 tctctggtta tgcgtgtagc gcaggattaa taaataaaat tgcaagtttc tccaggtaat
     4621 aaacatggcc gctatcccca tggcggcttt ttaatttaag aggcattaag atgaaaggat
     4681 tattatcttt actcattttt tctatggtcc ttcctgcaca tgccggaatt gttatctacg
     4741 ggacgcgcat tatttacccg gcagaaaata aagaagttat ggtgcagttg atgaaccagg
     4801 gaaatcgttc ttcgctgctg caggcgtgga ttgatgatgg cgatacgtcg ttaccaccag
     4861 aaaaaattca ggttcctttc atgttaacgc caccagtggc aaaaataggg gcaaattcag
     4921 ggcagcaagt aaaaatcaaa attatgccga ataaactgcc cactaataaa gaaagcattt
     4981 tttatctgaa tgttctggat attccaccaa atagtccaga gcaagaaggc aagaatgcac
     5041 tgaagtttgc gatgcaaaac agaattaagt tgttttaccg gccagcgggt attgctccgg
     5101 taaataaagc gacctttaaa aagttactgg taaatcgcag tggcaatgga ttggtgataa
     5161 aaaatgactc agctaattgg gtgacgattt cggatgtcaa agctaataat gttaaagtca
     5221 attatgaaac tattatgatt gcccccttag aaagtcagag tgttaatatc aaaagtaata
     5281 atgcaaataa ctggtatctg accattatca atgaccacgg caactatatt agtgacaaaa
     5341 tttaattgca ggagctgcct atgttgagaa tgaccccact tgcatcagca atcgtagcgt
     5401 tattgctcgg cattgaagct tatgcagctg aagaaacctt tgatacccat tttatgatag
     5461 gtggaatgaa agaccagcag gttgcaaata ttcgtcttga tgataatcaa cccttaccgg
     5521 ggcagtatga catcgatatt tatgtcaata agcaatggcg cgggaaatat gagattattg
     5581 ttaaagacaa cccgcaagaa acatgtttat caagagaagt tatcaagcgg ttaggcatta
     5641 atagcgataa cttcgccagc ggtaagcaat gtttaacatt tgagcaactt gttcagggtg
     5701 ggagctatag ctgggatatc ggggtttttc gtctcgattt cagtgtcccg caggcttggg
     5761 tggaagaact ggaaagtggc tatgttccac cggaaaactg ggagcggggt attaatgcgt
     5821 tttatacttc ttattatgtg agtcagtatt acagcgacta taaagcgtcg ggtaacaaca
     5881 agagtacata tgtacgtttt aacagcgggt taaatttact ggagtggcaa ctgcattctg
     5941 atgccagttt cagtaaaaca aataacaatc caggggtgtg gaaaagcaat accctgtatc
     6001 tggaacgtgg atttgcccaa tttctcggca cgcttcgcgt gggtgatatg tacacatcaa
     6061 gcgatatttt tgattctgtt cgcttcagcg gtgtgcggtt gtttcgtgat atgcagatgt
     6121 tgcctaactc gaaacaaaat tttacgccac gggtgcaggg gattgctcag agtaacgcgt
     6181 tggtaactat tgaacagaat ggttttgtgg tttatcagaa agaggttcct cctggcccgt
     6241 ttgcgattac agatttgcag ttggccggtg gtggagcaga tcttgatgtt agcgtgaaag
     6301 aggcggacgg ctcggtaacc acctatctgg tgccttatgc agcggtgcca aatatgctgc
     6361 aacccggcgt gtcgaaatat gattttgcgg cgggccgtag ccatattgaa ggggcgagca
     6421 agcaaagtga ttttgtccag gcaggttatc agtatggttt taataactta ttgacgctgt
     6481 atggcggcac gatggttgct aataattact atgcgtttac cctcggaacg ggttggaaca
     6541 cgcgcattgg cgcaatttcc gtcgatgcca cgaagtcgca tagtaaacaa gacaacggcg
     6601 atgtgtttga cgggcaaagt tatcaaattg cctacaacaa atttgtgagc caaacgtcga
     6661 cgcgttttgg tctggcggcc tggcgttatt cgtcgcgtga ttaccggaca tttaacgatc
     6721 acgtttgggc aaacaataaa gataattatc gccgtgatga aaacgatatc tatgacattg
     6781 ccgattatta ccagaacgat tttggccgta aaaatagctt ttctgccaat atgagccagt
     6841 cattgccaga aggctggggt tctgtgtcct taagtacgtt atggcgagat tactgggggc
     6901 gtagcggcag cagtaaggat tatcagttga gttattccaa taactggcgg cggataagct
     6961 ataccctcgc ggcaagccag gcgtatggcg agaatcatca tgaagagaaa cgttttaata
     7021 tttttatatc aattccctgt gattggggtg atgacgttac gacgcctcgt cggcaaatat
     7081 atatgtctaa ctcaacgacg tttgatgatc agggttttgc ctcaaataat acgggattat
     7141 caggaacagt agggagtcgg gatcagttca attatggtgt caacctgagt catcaacatc
     7201 agggaaatga aacgacagct ggggcgaatt tgacctggaa cgcgccggtt gcgacagtga
     7261 atggcagtta tagtcagtcg agtacttatc gacagactgg agccagtgtt tcagggggca
     7321 ttgtcgcctg gtcgggtggc gttaatctgg cgaaccgtct ttccgaaacg tttgctgtga
     7381 tgaatgcgcc aggaattaaa gatgcttatg tcaatgggca aaaatatcgc acaacaaacc
     7441 gtaatggagt ggtggtatac gacggaatga caccttatcg ggaaaatcac ctgatgctgg
     7501 atgtgtcgca aagcgatagc gaagcagaat tacgtggcaa ccggaaaatt gccgcccctt
     7561 atcgcggcgc ggttgtactg gttaattttg ataccgatca gcgcaagccc tggtttataa
     7621 aagcgttaag agcggatggt caaccattaa cgtttggtta tgaagtcaat gatatccatg
     7681 gtcataatat tggcgttgtc ggccagggaa gccagttatt tattcgcacc aatgaaatac
     7741 cgccatcggt taatgtagca attgataagc aacaaggact ttcatgcaca atcaccttcg
     7801 gtaaagagat tgatgaaagt agaaattata tttgccagta atagtcaggt ggttctatgg
     7861 aaattcgcat aatgctattt atattaatga tgatggttat gcctgtgagc tatgcggcat
     7921 gttatagtga gttatctgtt cagcacaatt tggttgttca ggggaatttt gcacttactc
     7981 aaacacaaat agcgacatat gagcataatt ttaatgattc gtcatgcgtg agtacaaata
     8041 ctatcacccc tatgagccca tcggatatta ttgttggact ttataacgat accattaaat
     8101 taaatttaca ttttgaatgg accaataaaa acaacatcac gttgtcaaat aatcagagca
     8161 gtttcaccag tggttattcg gtaacggtga cacctgcggc cagtaatgca aaagtgaata
     8221 tttctgcggg gggcggtggt tcagtgatga ttaatggtgt tgcgacatta tccagtgctt
     8281 catcatcgac acgcgggagt gccgcagtac aatttctact gtgtttatta ggtggcaagt
     8341 catgggatgc atgtgtaaat agctacagaa atgcattggc acaaaatgca ggtgtctatt
     8401 ccttcaatct gacattgtca tacaacccga taaccacaac ctgcaaacag gacgatttat
     8461 taattacttt agacagtatt cccgtttcac aattaccagc cacaggtaac aaagcaacaa
     8521 taaatagtaa aaaaagggat attattctgc gttgtaaaaa tttattaggt caacaaaatc
     8581 aaacatcacg gaaaatgcag gtgtatttat caagttctga cttgttaacc aacagcgaca
     8641 caatactgaa aggtgcggaa gataatgcgt aggatttatt cttgaaagta atggttcgcc
     8701 agtcacactt ttaaatatca ctaacagcag taaaggatac acaaatttaa aagaaattgc
     8761 ggcaaagtca aaacttacag atacaacggt ttcaattccg ataacagcca gttactacgt
     8821 ctacgataca aacaaagtta aatctggcgc actggaggca accgcattaa tcaacgtgaa
     8881 atacgactaa taacaagcaa gacgagcaag tggctaataa taaaaaataa caaggtgtct
     8941 gcatttcccc ctcatgatga gggggctttt tttagcgatg ataaaaaatc tcaccgtcgt
     9001 aggctttaat gattttaccg tcggtgtcgc tgatcaacac gtacgcgcca cccatatagg
     9061 tccagtgcgt cccggcatca ggcgcgggca ggttacgtaa ctgccactgc ttaatgttgt
     9121 actcatcggt gcggtacatc tctgggacgg tatcacctgg tttgaaatgc ttaaagtcag
     9181 caatgaaact acttaactca tacttagaaa ttcccgaggt attggtcgaa cccgcggtgg
     9241 ctggtgcggc ccaggcggca ctggtaacca gcaaaagcac acccagcaac atcttatttt
     9301 taatggtcat aaactctcca ctttggggat ctgtaatatc agtcgcatat ctttcctgcc
     9361 atacaatata actgacaagc gatattttgt tagtgatccc atgatttcgt aaaaagtgta
     9421 aatgaaagga tgccctaaaa gcatccttag cgagcagaga gtcagcggaa atagctgatt
     9481 taagaatcat cctgggctga agataacatg caagcctaaa ggattgagtt atcgcattat
     9541 gcccatgaag ccatgtacgc ccatatacac accgactaag ccaatcaaca gactggaaaa
     9601 atagggggcg cgtttagcga gagtgttaaa tccgctccag cgttttgcga cctgctgaac
     9661 gctgattgct gcgccaacgc ctacggtgac aagcgttaac gccaggccaa tgctgaaact
     9721 gacgaccagt gttgcgccca gtgtcagggc tttcaactga atgcatatca acagcacggt
     9781 aattgctgcc gggcagggga taaggccacc ggttaagcca aataacaaaa tttgccagtt
     9841 gatgacctct ctaccatcaa agcgtcgttt aatgtcattg gcatgggctc gtgcatgggc
     9901 atcctgatac tcgccatgtt cgtgatggtg atgatgtcct tggtcgtggt gatgttcgtg
     9961 gtcttgatga tgttcgtgat gatggtgatg atgctcataa tcatgcccgt gcatattctc
    10021 cagccagttg cgttcgccgc gccaggtacg ccagaacatc cagaacacgg tgccaataat
    10081 gatcactgcg gaaatcagct ggagccacgg ttctgctgat tgagcagtaa agcgcttact
    10141 gatcaccatc ccgccaaagg caatcaacca gaccaccgcg gtatgcgaaa tggttgctgc
    10201 cagtccgagc atcaccgcct gtttaatggt gcctttgatg gcgatgataa acgccgccat
    10261 catcgttttt gagtgccctg gttccaggcc atgaagcgca ccaagtaaga tggcgctggg
    10321 gatgaagaac caggcgtttc cttgctgaag aagagttgta aattcggtca tgataataat
    10381 tcttagtatt aattcggcaa tctgattcta ctccccccca gtacctgata ctacccccca
    10441 gtagattaat agtgctatga tttttcatat tcttgttaac caggtgttgc catgtctcat
    10501 acaatccgtg ataaacagaa actgaaagcg cgtgccagta agattcaggg ccaggtcgtg
    10561 gcgctcaaga aaatgctcga cgagccgcac gaatgcgctg cagttttaca acagattgct
    10621 gctatccgtg gagcggtaaa cggtctgatg cgggaagtga ttaaaggtca tctgacggaa
    10681 cacatcgttc accaggggga tgagctaaaa cgtgaagaag atctggatgt cgttctgaag
    10741 gtgctggatt catatatcaa ataatttatt aacgcgattg taaaactgcc gtttttcctc
    10801 gtttacaacg ggtgcgctgg acattaccat cctcctctgc gatttatcat cgcaaccaaa
    10861 cgactcgggg tgcccttctg cgtgaaggct gagaaatacc cgtatcacct gatctggata
    10921 atgccagcgt agggaagtca cggaccacca ggtcattgct tcttcacgtt atggcaggag
    10981 caaactatgc aagtcgacct gctgagttca gcgcaatctg cgcacgcgtt acaccttttt
    11041 caccaacatt cccctcttgt gcactgcatg accaatgatg tggtgcaaac ctttaccgcc
    11101 aataccttgc tggcgctcgg tgcatcgcca gcgatggtta tcgaaaccga agaggccagt
    11161 cagtttgcgg ctatcgccag tgccttgttg attaacgttg gcacactgac gcagccacgc
    11221 gcacaggcga tgcgtgctgc cgttgagcaa gcaaaaagct ctcaaacacg ctggacgctt
    11281 gatccagtag cggttggtgc gctcgattat cgccgccatt tttgtcatga acttttatct
    11341 tttaaaccgg cagcgatacg tggtaatgct tcggaaatca tggcattagc tggcattgct
    11401 aatggcggac ggggagtgga taccactgac gccgcagcta acgcgatacc cgctgcacaa
    11461 acactggcac gggaaactgg cgcaatcgtc gtggtcactg gcgaggtgga ttatgttacc
    11521 gatggacatc gtatcattgg tattcacggt ggcgatccgt taatgaccaa agtggtagga
    11581 actggctgtg cattatcggc ggttgtcgct gcctgctgtg cgttaccagg cgatacgctg
    11641 gaaaatgtcg catctgcctg tcactggatg aaacaagccg gagaacgcgc agtcgccaga
    11701 agcgaggggc caggcagttt tgttccacat ttccttgatg cgctctggca attgacgcag
    11761 gaggtgcagg catgaaacga attaacgctc tgacgattgc cggtactgat ccgagtggtg
    11821 gtgcggggat tcaggccgat cttaaaacct tctcggcact tggcgcttat ggttgctcag
    11881 ttattactgc actggtggcg caaaataccc gtggcgtaca gtcggtgtat cgcattgagc
    11941 ctgattttgt cgccgcccag ctcgattcgg tgttcagcga tgtgcgaatc gataccacta
    12001 aaatcggtat gttggcggaa accgatattg ttgaagcggt ggcagaacgg ttgcaacgtt
    12061 atcagatcca aaacgtggta ctcgacaccg ttatgctggc aaaaagcggc gacccgctgc
    12121 tttcaccttc ggcggttgct acgctgcgca gtcgattatt gccacaggtt tcattaataa
    12181 cgccaaactt gcccgaagct gccgccttgc tcgacgcgcc acacgcgcgc accgaacagg
    12241 aaatgctgga acaagggcga tcgctgttgg cgatgggctg tggcgcagtg ctaatgaaag
    12301 gtggtcatct ggatgatgag caaagcccgg actggctgtt tacccgcgag ggtgaacaac
    12361 ggtttaccgc accgcgcatt atgaccaaaa acacccacgg cactggttgt acactctctg
    12421 cggcgttggc tgcactacgc ccgcgccata caaactgggc tgacaccgta caggaggcaa
    12481 aaagctggct ttcatcggcg ttagcccagg ccgacacgct ggaagttggt cacggtattg
    12541 gtccggttca ccacttccac gcctggtggt gagcaccgta ctatagctgc tttggttatt
    12601 gtcagccttt ggcactgaca ttcagggatg aaaccaatgc agctaagaat taccagtcga
    12661 aaaaaactca ccgctctatt atgcgctctt gggctaattt ccatcgttgc aatttatccg
    12721 cgtcagacgg tgaatttttt ctattcgaca gcagttcaga ttacagacta catccacttc
    12781 tacggttatc gcccggttaa atctttcgct attcgcattc ctgccagtta caccattcac
    12841 ggattagatg tttcgcgctg gcaggagcgt atcgactggc agcgggtggc aaaaatgcgc
    12901 gacaacgata tccgcttaca gtttgctttt attaaggcta cggaaggcga aaagctggtg
    12961 gacccctatt tttcgcgtaa ctggcaatta agccgcgaaa atggcctgct gcgcggggcg
    13021 tatcattatt tctccccgtc ggtatccgct tcagttcagg cgcgattatt tctgcaaacg
    13081 gtggatttct cacaaggcga tttccctgcc gtgctggacg tagaagaacg gggaaaatta
    13141 tcggcaaaag aattacgcaa gcgggtaagt cagtggctaa aaatggtcga aaaacgcacg
    13201 ggaaaaaagc cgattattta ctcaggagcc gttttttatc acaccaatct ggcgggctat
    13261 ttcaatgaat atccgtggtg ggtggctcac tattatcaac gtcgtccgga caatgacggc
    13321 atggcctggc gcttctggca gcattccgac cgtggacagg tagatggcat caatggtccg
    13381 gtggatttta atgtgtttaa tggcacggtg gatgagttgc aggcattcgt tgatgggatt
    13441 aaagaaacgc cttaaataag aagcagtgat atactcccac cagactccct ggcaggaaaa
    13501 agacaaatgg aacaagcgca tacccagctg attgcccaac tgaacgagcg tattttagcg
    13561 gcggataaca cgccgcttta tattaagttt gccgaaacgg taaaaaatgc cgtgcgcagc
    13621 ggggtgctgg agcatggcaa tattttgccc ggtgagcgtg atttaagtca gttaaccggc
    13681 gtttcgcgca ttacggtgcg caaggcgatg caggcgctgg aagaagaggg cgtggtgacg
    13741 cgttcgcgcg gttacggcac gcagatcaac aacatcttcg aatactcgtt gaaagaagcg
    13801 agaggttttt ctcagcaggt ggtattgcgc gggaaaaagc ccgatacgct atgggttaat
    13861 aagcgtgtcg tgaaatgtcc cgaagaagtt gcgcagcagc tggcggtcga agcgggaagt
    13921 gatgtctttt tgcttaagcg tattcgctat gtcgatgaag aagcggtatc gattgaggaa
    13981 tcgtgggttc cggcgcattt aattcatgat gttgatgcca tcggaatttc tctttatgac
    14041 tatttccgca gccagcacat ttacccacaa cgtacgcgtt cccgcgttag cgcccggatg
    14101 ccggatgccg agtttcagtc acatattcag ttagatagca aaataccggt gctggtgatc
    14161 aagcaagtgg cgcttgacca acagcaacgg cctattgagt acagcattag ccactgtcgc
    14221 agcgatctat acgtttttgt gtgcgaggag tagttcctcg cgcgttggcg cacaatcacc
    14281 gccccgatgc ccgacaaccc acgacgccac tgcattgccc agtaatacgg catccgccag
    14341 tggcagacca gaggccagcc cggcaagtac gccacctgca tgactgtcgc ccgccccaat
    14401 ggtgtctaca acttgcgttg gaaatgcccg aatgcagcca gaagcgtcgt tgctgaaata
    14461 ccatgcgcct tctttatcga gacgaacgat caacggcgcg gcaaatttct cctgccattg
    14521 cttgccaagt gttgttatct ctgcagataa agcaaaacgt tcggcggcaa tctcagcctc
    14581 ttgacgattg agtgacacta aaggtcgaca ggccatgatc cgcgccagta atgcgtccgg
    14641 gatatcgcca atacgtgggc caaaatcgat aaacggcgtc acgtcttgca gcttttccag
    14701 ccattccact aacaattcgc cgcagggcga ggccagttga taaccggaaa aatagagcag
    14761 gttgccaggc gcaacggtta atcgtgccaa ccattggcga ttccattgat tttccacacc
    14821 gctgaatgac ataaaagtgc gttcgccatc cggctcaacc agtgccagac accagccgtt
    14881 atcgctttcg gcgttatcga tcaggctgat taacccctct ttcgccatcc ggttgcgaat
    14941 aatctccgcc cacacgccct gaccgagcgg caaggcatta cccgcttcga tgccgaggcg
    15001 ctttaacgcc acggcaatat tcagtgcgca gccgccaacg ttaacgctct gctgtttcag
    15061 ttcgatatcg cacccacgcc aggggagggc ataagcgtcg gcgatcacgt caatgaccgc
    15121 cgcgccgacg accatcaccg gctgacgcgt ggttaattca ggcagcagtg tgtgtaatcg
    15181 agcgccgctc ataccgcctc ccgttgttga cgatatttct ccagcgctgt ggcatagcgg
    15241 ttgaaatcaa gctgatttac cgcatccagc tccgccttta acgcaggatc gacagcgtta
    15301 atgccatgca acgcgccgca aatcgccgtc gccatagcac ctatggtgtc tgtgtcgccg
    15361 ccaagattgg cgcacaggac ggcgcagcgg tttgggtcgg tttgtgccag ttcaactagc
    15421 gcaatggcgc acggaacgga ctcaatagtg ctggtgcctg cgccaacgac ctggtaaagc
    15481 tgttcgctgg cggattcggt gccgtcggca ttacgcacaa ttttcagcgc aatctccaga
    15541 cgtgccgcca gtgaggcgct gaacgtggtg gtgcgttttt gctgtgcatg tcgcgcaatt
    15601 gaaggcaatg aatcaacaat cgctgaccag cgttctccgt caatggcacg agaaatcgcc
    15661 catgcgatga ctaccgcgcc tgcaaccgcc agatcggatt tatgggtcgg gctggacgcc
    15721 agcgccacat catcaataaa ggaatcaaca tcacgcgctg gaagcaaaca acctaatggc
    15781 gagacgcgca tcgctgcgcc gttggttacg ccgttgtttt ccagttcagc aacgggttta
    15841 ccgtcgcgaa tggcgttaag cgcaatcttc gaggtcggac ctagtacgtt tttgttaaag
    15901 gcgtcgaaac gcaacgccca gtcgagaata ttacgcccaa tcagattcgg atcgatcttg
    15961 ccttcacatt ccagtaacgc atccgccaga cacaggccat cgaggtatcg tcggtgaatt
    16021 cggcgcggtt aaaataacag gccgcgttat tctcctttgg tccaggcaga aaacggtcaa
    16081 tccagccaaa gtgtgcttta acgcggctgc gtggccaaag ctcggagggc atccccatcg
    16141 catcccctaa cgcctgccca taaagagcac cgagaatacg ttctgttttc atttaacttc
    16201 cccttgtgtc aacgcaatat ctcgatcatc gaccttgata gccgtaattt cgttgtcgga
    16261 ttcgcgggaa aaaatcatga acagcacggc gataatggca atcatcaccg cgccgaaagt
    16321 ccacatcccg gaccagttga aagtcagtcc gtttaccggt tcctgataag cgaacatctt
    16381 ttccgtcatc acaccgccaa gacgatagcc gagcaaactg ccgaagccct ggcagcagag
    16441 cgtgatcagc ccctgcgcag cggtacgcat atgcacgggg gcttttttat cgacatagat
    16501 gtaagcggta acgtagtaaa aatcgtaact tacgccgtga agcaaaatac cgaggaacag
    16561 taacgcgtag gtgaaattga caggtaatgt aaggtacgcg atgacaatac cacataataa
    16621 taccttttga atacctttgc ggtaaaaact tgaccgcgct aacatttttt ctgatgaatc
    16681 gagccaacag aaaacgctga aaaaacatcc aaaagatgga aaaactcgcc cgtaacgacc
    16741 aggattatga ttgcccgcaa aaggcgggcc aggacaatcc atagccgata tccaatcgga
    16801 atttacggga gcatagtaat gacagatatt gcgcagttgc ttggcaaaga cgccgacaac
    16861 cttttacagc accgttgtat gactattcct tctgaccagc tttatctccc cggacatgac
    16921 tacgtagacc gcgtgatgat tgacaataat cgcccgccag cggtgttacg taatatgcag
    16981 acgttgtaca acaccgggcg tctggctggc acaggatatc tttctattct gccggttgac
    17041 cagggcgttg agcactctgc cggagcttca tttgctgcta acccgctcta ctttgacccg
    17101 aaaaacattg ttgaactggc gatcgaagcg ggctgtaact gtgtggcatc aacttacggc
    17161 gtgttggtgt cggtatcgcg gcgctatgcg catcgcattc catttctcgt caaacttaat
    17221 cacaacgaga cgctaagtta cccgaatacc tacgatcaaa cgctgtatgc cagcgtggag
    17281 caggccttca acatgggcgc ggtggcggtt ggtgcgacta tctattttgg ctcggaagag
    17341 tcacgtcgcc agattgaaga aatttctgcg gcctttgaac gcgcgcacga gctgggcatg
    17401 gtggcagtgc tgtgggccta tttgcgaaac tccgccttta agaaagatgg cgttgattac
    17461 catgtttccg ccgacctgac tggtcaggcg aaccatctgg cggcaaccat aggtgcagat
    17521 atcgtcaaac aaaaaatggc ggaaaataac ggcggctata aagcgattaa ttacggttat
    17581 accgacgatc gcgtgtacag caagttaacc agcgaaaacc cgattgatct ggtgcgttat
    17641 cagttagcta actgctatat gggccgggct gggttgataa actccggcgg tgctgcaggc
    17701 ggtgaaactg acctcagcga tgcagtgcgt actgcggtta tcaacaaacg cgcaggcgga
    17761 atggggctga ttcttggacg taaggcgttc aagaaatcga tggctgacgg cgtgaaactg
    17821 attaatgccg tgcaggacgt ttatctcgat agcaaaatta ctattgcctg atacgcctca
    17881 tacgaacagc accaccccgt aggacggata aggcgtttac actgcatccg gcaacctatg
    17941 cctgatgcga cgcggaagcg tcttatcatg cctacatagc actgccacgt atgtttacac
    18001 cgcatccggc ataaaaacac gcgcactttg ctacggcttc cctatcggga ggccgttttt
    18061 ttgcctttca ctcctcgaac aattttcata ttgtcgtttt tgtgatcgtt atctcgacat
    18121 ttaaaagcaa ataatttcat tattttttga aatcgaaagc aaacaacaag atatgaaaat
    18181 gtacgtggta tcgacaaagc agatgctgaa caacgcacag cgcggcggtt atgcggttcc
    18241 ggcattcaat attcacaatc tcgaaacgat gcaagtggtg gtggaaaccg ctgccaacct
    18301 gcatgcgccg gtcatcatcg ccggaacgcc tggcacattt actcatgctg gtacagaaaa
    18361 tctgttggcg ctggtcagcg cgatggcgaa gcaatatcac catccactgg caattcatct
    18421 cgaccatcac acgaaatttg acgatatcgc tcagaaggtt cgttctggcg tgcgctcagt
    18481 catgattgac gcctcgcatt tgccttttgc gcaaaatatt tcacgggtca aagaggtggt
    18541 ggatttttgc catcgctttg atgtcagcgt cgaggcggag ctggggcaac ttggcggcca
    18601 ggaagatgat gtgcaagtca atgaagccga tgcgttgtac accaaccccg cacaggcgcg
    18661 tgaatttgcc gaggcaaccg ggattgattc cctggcggtc gccatcggca cggctcacgg
    18721 tatgtatgcc agcgcaccgg tgcttgattt ttctcgactg gagaacattc gccagtgggt
    18781 gaacttaccg ctggtgctgc atggcgcgtc aggattatcg actaaggata ttcagcaaac
    18841 catcaaactg gggatatgca aaatcaacgt tgcaacggag ctgaaaaatg ccttctcgca
    18901 gtcgttaaaa aattacctga ccgagcaccc cgaagcgacc gatccccggg attatttgca
    18961 gtcggctaaa tccgcaatgc gcgatgtggt gagcaaagtg attgccgatt gtggctgcga
    19021 gggcagggca taacgcacct gccatttaac aaggaaaaaa catgaaaacg ttaattgccc
    19081 ggcataaagc tggtgaacat atcggcatat gttcagtctg ttctgcccat ccgttggtta
    19141 tcgaagcggc gctggcattt gatcgcaaca gcacgcgcaa agtgctgatt gaagcaacgt
    19201 caaaccaggt caatcaattt ggcggttata ccggaatgac actggcagac tttcgcgaat
    19261 ttgtttttac gattgccgat aaagttgggt ttgcacgcga acgcattatt ctcggcggcg
    19321 atcatctggg gccaaactgc tggcagcaag aaaatgcgga tgcggcgatg gaaaaatccg
    19381 tcgagctggt aaaagcatat gttcgtgctg gcttcagtaa aattcatctt gatgcgtcaa
    19441 tgtcctgcgc gggggatccc ataccgttag caccagaaac ggttgcggaa cgagctgctg
    19501 tgctttgctt tgctttgctg cggaaagtgt ggcgacagat tgccagcgtg agcaactgag
    19561 ctatgtcatt ggcactgaag ttccggttcc gggcggtgag gccagcgcca ttcagtcagt
    19621 acacatcacc catgttgaag atgccgccaa tactttacgt acgcatcaaa aggcctttat
    19681 tgcccgtggg ctgacagagg cgttaacacg tgtgattgcc atcgtggtgc agccgggtgt
    19741 ggaatttgat cacagcaata ttatccatta tcagccgcag gaagcgcagg cgctggcgca
    19801 atggatagaa aacacccgaa tggtttatga agcacattct accgattacc agacccggac
    19861 ggcttattgg gaattagtcc gcgatcactt tgcaatattg aaagtcggtc ctgcattaac
    19921 ctttgcttta cgcgaggcga tatttgcact ggcacaaatt gagcaggaac ttatcgcccc
    19981 cgaaaatcgc agcggttgcc tggcggtaat tgaagaagtg atgctcgacg aaccgcaata
    20041 ctggaaaaaa tattatcgca cgggttttaa cgattcatta ctggatattc gttacagcct
    20101 gtcggatcgt attcgttatt actggccgca tagtcggatt aaaaatagcg ttgaaacgat
    20161 gatggtgaat ctgcaaggcg tggacatccc actgggcatg attagtcagt atcttcccaa
    20221 acaatttgaa cgcattcagt ccggggaatt atcagcaatg ccgcatcagc ttattatgaa
    20281 taaaatttat gatgttttgc gcgcctatcg ctacggttgt gcggaataag gacggtatat
    20341 gactaacctg tttgttcgta gcggaatttc ttttgtcgat cgtagcgaag ttttaaccta
    20401 tatcggtaat gagatgctcg cgaaaggtgt ggttcatgat acctggccac aggcattaat
    20461 tgccagagaa gcagaattcc ctaccgggat aatgcttgag cagcacgcca ttgcaatacc
    20521 gcattgtgag gcgattcatg ctaagtcgtc agccatttat ctgttaaggc caacaaataa
    20581 agttcatttt cagcaagcgg atgatgataa cgacgtggcg gtatcgttgg ttattgcgtt
    20641 gattgtggaa aatccgcagc agcaattgaa acttttacgc tgtttatttg gcaagttaca
    20701 acagcccgat atcgtcgaga cactaattac tcttcctgaa actcagttaa aggaatactt
    20761 cacaaagtat gttttagatt cagacgaata aatccctctg taacaataaa aaggactatt
    20821 tatgaaacgc aagattattg tcgcttgcgg aggcgcggtt gcgacctcta cgatggcggc
    20881 ggaagaaatt aaagagttgt gtcagagtca taatattcct gttgaattaa tccagtgtcg
    20941 ggttaatgaa atagaaacct atatggatgg tgtgcatttg atatgtacca ctgccagggt
    21001 ggatcgtagt tttggcgata ttccgttagt tcacggcatg ccttttgttt ctggtgtcgg
    21061 tatcgaagca ttacaaaata aaattctgac tatcttacag gggtgaccta tgttttcaga
    21121 agtcatgcgt tatattctcg acctcggccc tacagtgatg ctgccggttg tcatcattat
    21181 tttttctaaa atattaggca tgaaggcagg cgattgcttt aaagcgggtc tgcatatcgg
    21241 gattggcttt gttggcattg gccttgtgat tggcttaatg ctggattcca ttggtccggc
    21301 ggcgaaagcg atggcggaaa atttcgacct gaatctgcat gtggtcgatg tcggctggcc
    21361 gggctcttca ccaatgacct ggacgtcgca aattgcgctg gtggcgattc cgattgcgat
    21421 attggttaac gtggcgatgt tactgacccg tatgacgcgg gtggtaaatg ttgatatctg
    21481 gaatatctgg catatgacct tcaccggcag cgttgctgca cctggcaacc ggttcatgga
    21541 tgatagggat ggcgggtgtg gtaattcacg cggcgtttgt ttataagctc ggcgactggt
    21601 ttgcccgcga tacccgaaat ttctttgagc tggaaggtat tgctattccg cacggtacgt
    21661 cggcgtatat ggggccgatt gcggtgctgg tcgatgctat catcgagaaa atcccaggcg
    21721 ttaaccgaat taaatttagc gccgacgata ttcagcgcaa atttggccct tttggcgaac
    21781 ctgtcaccgt gggttttgtg atggggctga ttatcggcat cctcgcgggt tacgatgtca
    21841 aaggtgtatt gcagctggcg gtaaaaacgg cggcagttat gctgctgatg ccacggatga
    21901 ttaaacccat catggatggt ttaacgccca tcgctaagca ggcccgtagt cgtttacagg
    21961 cgaagttcgg cggtcaggag ttcctgattg gcctggatcc cgcattactg ctggggcata
    22021 cggcggtggt atcggcaagc ctgattttta ttccgctcac cattttaatt gctgtttgtg
    22081 ttccgggtaa tcaggtactg ccgtttggcg atcttgccac tatcggcttc ttcgtggcga
    22141 tggcggtcgc ggtgcatcgt ggaaatctgt tccgcacctt aatct
//
LOCUS       DAJVQH010000061        22004 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJVQH010000061 DAJVQH010000000
VERSION     DAJVQH010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.061"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..161
                     /locus_tag="OQM87_002347"
     CDS             <1..161
                     /locus_tag="OQM87_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCS1508458.1"
                     /translation="TLSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNS
                     STAELLLK"
     gene            231..1037
                     /locus_tag="OQM87_002348"
     CDS             231..1037
                     /locus_tag="OQM87_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1508459.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            1047..1454
                     /locus_tag="OQM87_002349"
     CDS             1047..1454
                     /locus_tag="OQM87_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCS1508460.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1454..1948
                     /locus_tag="OQM87_002350"
     CDS             1454..1948
                     /locus_tag="OQM87_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCS1508461.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            2014..2811
                     /locus_tag="OQM87_002351"
     CDS             2014..2811
                     /locus_tag="OQM87_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCS1508462.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2822..3610
                     /locus_tag="OQM87_002352"
     CDS             2822..3610
                     /locus_tag="OQM87_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCS1508463.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3662..4891
                     /gene="sorE"
                     /locus_tag="OQM87_002353"
     CDS             3662..4891
                     /gene="sorE"
                     /locus_tag="OQM87_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCS1508464.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMELSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5273..5962
                     /gene="pepE"
                     /locus_tag="OQM87_002354"
     CDS             5273..5962
                     /gene="pepE"
                     /locus_tag="OQM87_002354"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCS1508465.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6053..7684)
                     /locus_tag="OQM87_002355"
     CDS             complement(6053..7684)
                     /locus_tag="OQM87_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCS1508466.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7904..11587)
                     /gene="metH"
                     /locus_tag="OQM87_002356"
     CDS             complement(7904..11587)
                     /gene="metH"
                     /locus_tag="OQM87_002356"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCS1508467.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISSDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTMPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11787..12611
                     /gene="iclR"
                     /locus_tag="OQM87_002357"
     CDS             11787..12611
                     /gene="iclR"
                     /locus_tag="OQM87_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCS1508468.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12928..15112
                     /gene="espL4"
                     /locus_tag="OQM87_002358"
                     /pseudo
     CDS             12928..15112
                     /gene="espL4"
                     /locus_tag="OQM87_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(15081..16817)
                     /gene="aceK"
                     /locus_tag="OQM87_002359"
     CDS             complement(15081..16817)
                     /gene="aceK"
                     /locus_tag="OQM87_002359"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCS1508469.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(17001..18305)
                     /gene="aceA"
                     /locus_tag="OQM87_002360"
     CDS             complement(17001..18305)
                     /gene="aceA"
                     /locus_tag="OQM87_002360"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCS1508470.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGESMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(18335..19936)
                     /gene="aceB"
                     /locus_tag="OQM87_002361"
     CDS             complement(18335..19936)
                     /gene="aceB"
                     /locus_tag="OQM87_002361"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCS1508471.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(20205..21134)
                     /gene="metA"
                     /locus_tag="OQM87_002362"
     CDS             complement(20205..21134)
                     /gene="metA"
                     /locus_tag="OQM87_002362"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCS1508472.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            21291..21731
                     /locus_tag="OQM87_002363"
                     /pseudo
     CDS             21291..21731
                     /locus_tag="OQM87_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
     gene            complement(21848..21963)
                     /gene="rrf"
                     /locus_tag="OQM87_002364"
     rRNA            complement(21848..21963)
                     /gene="rrf"
                     /locus_tag="OQM87_002364"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5412 a         5642 c         5570 g         5380 t
ORIGIN      
        1 aaacgctctc tatcgaaatg aataaattaa agcaggcgcg atattccatt ggcattgcca
       61 tgagtgaaga aaaatacagc ggaattattg gtgcactgcg tggaaaatat attaattgtc
      121 tggtaacgaa tagcagcaca gctgaactat tactgaaata aaaatcttcc cgatgtttac
      181 cgtaataaaa attacggcgg gtccctatta tctaaaatca ggagtctgtt atgcaaacgt
      241 ggttaaattt gcaggataaa atcattattg tcaccggcgg cgcatccggt attggtctgg
      301 cgattgtaga ggaattatta gcacaaggcg cgaatgtaca gatggtcgat attcacggtg
      361 gcgatggtca atatgaaggt cataaaggct atcagttttg gccgaccgat atttctagca
      421 caaaagaggt aaatcatacg gtagcggaaa ttatccagcg ttttggtcgc atcgacggtc
      481 tggtcaataa cgccggggtc aatttcccgc gtctgctggt cgatgagaaa gcgcctgccg
      541 ggcagtatga actcaacgaa gctgcattcg aaaaaatggt caatatcaac cagaaaggtg
      601 tttttctgat gtcgcaggcg gtggcgcgac agatggtcaa acaacatgat ggcgtgattg
      661 tgaatgtttc ctcagaaagt gggctggaag gctcagaagg acaaagctgt tacgccgcta
      721 ccaaagccgc gctcaatagc ttcacgcgct cctggtcgaa agagctgggt aaacacggta
      781 tccgtgtggt cggtatcgcg cccgggattc tggaaaaaac cgggctacgc acgccggaat
      841 atgaagaggc gctggcgtgg acgcgcaata tcaccgtcga gcagctgcgt gaaggctata
      901 ccaaaaacgc cattcctatt gggcgcgccg gaagattagc agaagtagct gattttgttt
      961 gttatctgtt gtctgaacgc gccagctaca tcaccggagt aaccactaac attgcgggcg
     1021 gcaaaacgcg cggctaagga ggctttatgg tcaacgccat tttttgtgcc cacggcaaat
     1081 tagcttgcgc catgctggaa tcggttcaga tggtttacgg caacgccaat gttgaagccg
     1141 ttgaatttgt gcccggtgaa aacgccggag acattgcggt aaagctggaa aagttagcaa
     1201 gcattcacaa tcaagatgag tggctgattg ccgtggattt acagtgcggc agcccgtgga
     1261 atgcggcagc aatgcttgcg atgcgcaatc ctcgtttgcg ggtaatcagc ggcctttctc
     1321 tgccgctggc gctcgaactg gttgataacc aggacagcat gaatgttgat gaactgtgtg
     1381 aacacctgac gcaaatcgca aagcaaacct gcgttgtctg gaaacaactg gcaacgacag
     1441 aggaggattt ctgatgaaca ttacgctcgc ccgcattgat gaccgtctga tccacggcca
     1501 ggtcactacc gtctggtcga aagtggctaa cgcccagcgc attattatct gcaatgacga
     1561 agtttataac gatgaagtgc gccggacatt attgcgtcag gctgcgccac cgggaatgaa
     1621 ggttaacgtg gtcaatattg aaaaagccgt cgcggtttat cacaacccgc aatatcagga
     1681 cgaaaccgtt ttttatcttt tcactcgccc acaggatgcc ttagcgatgg ttcgccaggg
     1741 ggtaaaaata ggcaccctga atattggcgg catggcctgg cggcccggta aaaaacagtt
     1801 aaccaaagcg gtttcattag atgatgatga tattaacgca tttcatgagt taaataatct
     1861 tggtgtcata ctggatttac gcgtcgtagc gtcagatccc tcaatcaata ttatcgacaa
     1921 aataaacgaa cagttaatcg caaattaaat aatagcgcct gtgtacatat gctttaacag
     1981 gcagggatga cgacacttaa aaggtgacaa atcatggaaa taagtaccct acaaatcatt
     2041 gctatatttc ttttttcctg tattgccgga atgggcagcg tgctggatga atttcagact
     2101 catcgcccgt taattgcctg tacggtgatt ggtttaattc tcggtgattt aaaaaccgga
     2161 ataatactcg gcggtacgct ggaattgata gctctcggct ggatgaacgt cggcgcggcg
     2221 caatctccgg attctgcact cgccagcata atctccgcca ttctggttat cgttggtcag
     2281 caaagcatcg ccaccggaat cgccatcgcg ttgcctgtgg ctgcggcagg ccaggtgctg
     2341 acggtgtttg cccgtaccat caccgtagtg ttccagcacg cggcggataa agcagcagaa
     2401 gaggcgcggt ttcgcactct cgatattctg catgtctccg cgcttggcgt gcaggcgctg
     2461 cgcgtcgcca ttccggcact gattgtctca ctgttcgtca gcgccgatat ggtcagcaat
     2521 atgttaaacg ccattcccga atttgtgacc cgaggactac agattgctgg cggttttatc
     2581 gtggtggtcg gttacgccat ggtgctacgt atgatgggcg tgaaatattt gatgcctttc
     2641 tttttcctcg gtttcctcgc aggtggctac ctcgatctca gcctgctggc gttcggtggc
     2701 gtcggcgtga tcatggccct ggtctacatc cagctaaatc cacagtggcg taaagctgaa
     2761 cctcaactcc aggccaccgc ctccaccgcc cttgaccaac ttgacgatta acggagccat
     2821 catggaacag agaaaaatta cacgcagcga tctggtgagc atgtttctgc gctccaacct
     2881 gcaacaggcg tcctttaact ttgaacgtat tcacgggctg ggcttttgct acgacatgat
     2941 ccccgccatc aagcgacttt acccattaaa agaggatcag gttgcggcgc tcaggcgaca
     3001 cctggtgttc ttcaatacca cgccagccgt atgtggcccg gtcatcggcg cggaaattga
     3061 tgacggtacc atcaacggca tcaaagtcgg tctgatggga ccattggcag gagttggcga
     3121 tccactggtc tggggaacgc tgcgcccgat taccgccgcg ctcggcgcat ctctggcact
     3181 ttcgggcaac attctcggcc cgctgctgtt cttctttatt ttcaacgcgg tgcgtctggc
     3241 gatgaagtgg tatggcctac agctcggctt tcgcaaaggg gtgaatatcg tcagcgatat
     3301 gggcgggaat gtgctgcaaa aactcaccga aggcgcgtcg attctcggac tgtttgtgat
     3361 gggcgtgctg gtgaccaaat ggacgtcaat caacgtaccg ttggtggttt cacaaacgca
     3421 tgccgccgat ggctccaccg tcaccatgac cgtgcagaac attctcgacc aactttgccc
     3481 tggtttgctg gcgctcggtc tgacgctact aatggttcgt ctgctcaaca aaaaaattaa
     3541 cccggtatgg ctgattttcg ccctgtttgg cttagggatt atcggcaatg ctctgggctt
     3601 cctgtcctga ttcttcgccc cggcacgact gccgaggcca tcgctcaaca tgaggtggtt
     3661 tatgaaaaca acagctctgc gtctttatgg taaacgtgat ttacgcctgg aaacctttga
     3721 ccttcctgaa atgcaggagg atgaaatcct cgcaacggtg gtcactgaca gcctgtgcct
     3781 ctcttcctgg aaagaggcca atctgggtga aaaccataaa aaagtacccg acgatgtggc
     3841 gaccaaccca atcatcatcg gccacgaatt ttgcggcgat attctggccg tgggtaaaaa
     3901 gtggcagcac aaattccagc cgggtcagcg ttatgtgatt caggccaacc tgcaactccc
     3961 cgaccgcccg gattgccccg gctactcctt cccgtgggta ggcggcgaag cgacgcatgt
     4021 ggttattccc aacgaggtca tggaacaaga ttgcctgctg gcatacgacg gcgaaaccta
     4081 ttttgaaggc tcactggttg aaccgctttc ctgcgtgatt ggcgcgttca acgccaacta
     4141 tcatcttcag gaaggtagtt ataaccacac gatggggatt cgcccgcaag ggcgcacgct
     4201 gatcctcggc ggcaccggac caatgggact gttggcgatt gattatgcgc tgcatggacc
     4261 agttaacccg tcgctgctcg tcattaccga taccgacaac gacaaattga gttatgcgcg
     4321 caagcactat ccatcagaac cgcaaacact gattcattat ctcaatgcca ccgatgcagc
     4381 atttgatacg ttaatggagc tgagtggcgg tcacggcttc gatgatattt tcgtctttgt
     4441 gcctaatgaa ggactggtga ctctcgcctc ttccttgctg gcgacagatg gttgcctgaa
     4501 tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca attaatttct acgatgtgca
     4561 ttatgcattt acccactacg tgggcacgtc aggcggcaat accgacgaca tgcgcgcggc
     4621 ggtcaaactg atcgaagaga aaaaagtgca ggccgcaaaa gtggtaacac atattcttgg
     4681 gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc gtcggcggcg gcaaaaagct
     4741 ggtgtatacc gggaaatacc tgccgctgac gtcactcacg cagattcagg atcaagcact
     4801 ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt gaggcggagc aatacctgct
     4861 cgcccatgca gaggcaattt cccatgatta atcgcgctac ttaactctgc atgtcgctgg
     4921 ctggtcgccc cagcaacttc gcgaccggat gtgcggctga ttgctgaagc agcacaattt
     4981 gcacgagaag gataaaaaat atcagcgcct taaggggcgc tcaaattggt ggcaaagtac
     5041 acattgttta tgccggatgc ggaatgaagc cttattcggt ctacataatt ttgaaaatcc
     5101 aatatattgc agttatctgg taggccaggt aagcgtagcg catcaggcaa tgctgtgttt
     5161 gtcatcagtc tcagcgcccg gtgcaatacg tgacatcgtg agccatgtca gtaagtggtt
     5221 aaattcgcag tggtagcatg ccttccgtga tttcttacta accggagaag taatggaact
     5281 gcttttattg agtaactcga cgctgccggg taaagcctgg ctggaacatg cactgccgct
     5341 aattgctgaa cagttgcagg gtcgccgctc agcggtgttt atccctttcg ctggcgtaac
     5401 gcagacctgg gatgattaca cagagaaaac ggctgcggtt ctcgctccgc tgggtgtttc
     5461 tgtcaccggt attcatagcg ttgtcgatcc cgttgccgcg attgaaaatg cggaaattgt
     5521 gattgtcggc ggcggtaata ctttccagtt gctgaaacag tgccgcgagc gcgggctgct
     5581 ggctccaatt actgacgtgg ttaaacgtgg cgcgctgtat attggctgga gcgcaggcgc
     5641 taaccttgct tgcccaacca ttcgtaccac caacgatatg ccgattgtcg atccgcaagg
     5701 tttcgatgcg ctaaatctgt tcccgttgca aatcaacccg cacttcacca acgcgctgcc
     5761 ggaaggccat aaaggtgaaa cccgtgagca gcgtattcgc gaactgctgg tcgtcgcgcc
     5821 agaactgacg attattggtc tgccggaagg taactggatc acagtgagta aaggtcacgc
     5881 tacgctgggt ggcccgaaca ccacttatgt gtttaaggct ggtgaagaag cggttccgct
     5941 ggaagctggt caccgttttt aagtgaattg ccggatgcat gatccggcat cttgttcata
     6001 gtgcctgatg cgacgcttga cgcgtcttat caggcctaca ggattcacaa tattaatact
     6061 cgtcccgtcc ttcatcttca tccggctgtt ccagcacact gtaagccacc gaacaaaaca
     6121 gcgagttcag gcgctgcata tctcccagta agcctaaatg tagcgaactg gtttcaatgc
     6181 tctgcacgtt ttgctgatgc aggcgatcga cgtgggcgtg ggaatagcgg cgattaagaa
     6241 tgcgaaaacg atgtttgctg cgacgcaaac gacgagcgct ggtgacatcg ccagagaaga
     6301 acaccgacat tgccagcttt aaattactga gcaattgctc atagagcgca tccagttcct
     6361 tcaacccatc aagcgaaaat gcccgccgtg ctgccagcga tttatcggca atttcgctgc
     6421 ccatgcgctc gacgatatcg gaggcctgtt caaggttgag cgacatttcg atgatctccg
     6481 cccagcggcg cgactcttcc tccgccaact cttcttttgg catccgcgcc agatacagct
     6541 taatggcggt gtagagaacg ttgatatcat ctgccagctt acgcagctct ttctcctgcc
     6601 gtggctcgcc gtgcatcact ttattcagcc cttccatcat ctgttccatg gcgtcgccaa
     6661 tgcgcagggt ttcgcgcgcg gcgttcgcca gagcaagcgt gggcgtgtcc agcgcgctga
     6721 catccagatg tttaggccgt agctgggtat ccagttccgg ttcatcgcga ataatcgttt
     6781 tgcaaaaccg tgccatcggg tcaacaaatg gcagcatgac caggcaacgt acaaggttgt
     6841 agaagacgtg gaaatagatc accagttccg cttttggcaa tgacagcttc cccattgtct
     6901 ctgccagcaa atggacaaac ggcaggataa tcaggctacc caccagctta aacagcagac
     6961 tacccagcgc gacacggcgg gctgcggcat tggcggcact gttgttgagc atcgccagca
     7021 ggccggaacc gaggttagct ccaatcacca gacagagcgc cacggggaag gagataatgc
     7081 ctgcagcggt cagagtcgcg gtcagcagta cagcagcaag gctggagtag ctgataatgg
     7141 cgaacatcgc gccaatcagc gcatccagca gaatatcgcc ggtcagcgag gcaaagatca
     7201 cctgaacgcc gtttgcctgg gtgatcggcg ttacggcctg cacaatcaac tccagcgcca
     7261 gcaaaatcag cccaagacca ataccgacgc ggcccagttg cccggcgcgt gactgtttgc
     7321 gtccgaggaa aaaaatcacg ccgataaaaa taagtaacgg tgacagccag gataagtcga
     7381 aggtgagaat acgcgccatt agcgccgtcc cgacatctgc ccccagcaca atgaccagag
     7441 ccggtgcgag ggctaccaga tcctgagcga cgaacgaggt caccagcatg gtggtggcat
     7501 tactgctctg taccagtgcg gtaacgccga tccccgcgca aaaggcgagc ggcttttttt
     7561 cgacgctccg gctaaggaca gtacgcaaac gcgcgccgaa gacgcgcatt acgccggttc
     7621 gaacaatatg agtcccccag accagcaggg cgacggcaga aagcaggtga agcagcgtta
     7681 acacgagttc aggttctcct tatcgttata tgttcctgaa gcaattgaat gaccaaagga
     7741 ccggaaacac gctgactggc atctttactg tgccttttga cattcgtttt tccggctaag
     7801 attattccag cctgtttcca gtataagtgt ttcatattaa taaagagaca gggcactcat
     7861 tgagcgccct gtttgttgta aggaaaagtg acagatttgt gaatcagtcc gcgtcatacc
     7921 ccagattcgg tgccagccag cgctcaacat cggagacgct cataccttta cggcgggcat
     7981 agtcttcaac ctgatcgcgc tgaatttgcg ccaccgcgta gtacttgctg tccggatgac
     8041 tgaaatacca acccgaaacc gatgcaccag gccacatagc gaaagattct gtgagtttca
     8101 tgccagtatg tttttccact tccagcagct cccagatggt ggctttttcc gtatgttccg
     8161 ggcaggccgg atagcccggt gccggacgga tgccctggta gttttcgcgg atcagttctt
     8221 cgttgctgag gttctcgttc ggcgcatagc cccagtagac tttacgcaca cgctcatgga
     8281 gatactccgc aaaggcttcg gctaaacggt cggcaagcgc tttcaccatg attttgttgt
     8341 aatcatcgtg ctgcgcttca aaggcatcag ccagtgcgtc ctcttccagc ccgccagtca
     8401 cggcaaatgc gccgatgtaa tctgctttac cagaaagctt cggcgcaacg aagtcagcga
     8461 gacagtagtt agcgaatccc gttttttcgg tctgttgacg cagatggtgg ctgacgttga
     8521 tcacatgggt gcgcgtttcg tcacggtaga tttcaatgtc atcgcccaca cggtttgccg
     8581 ggaacaggcc caccacgcca cgcggattca gcgttttctc ggcgcttaat ttatccagca
     8641 tgtcgttggc gtctttaaac agccgctgcg cctcaacgcc caccacttca tcttccagaa
     8701 tgcgcggata cttcccggcc agcgaccagg tcataaagaa cggtgtccag tcgatgtaat
     8761 tacgcagcgt ttcgatgctg gcttccactt cctgcacgcc gagacggtgc gccaccggcg
     8821 gcgtgtaagc ctgccagtca aatgcgaaat cgttatcgcg cgccgcttcc agcgtgaccg
     8881 gtggtgtgcg tggtttcttg cgcccgtgct gaatacgcac ggtttcgtac tccttgcagg
     8941 tacgagcgac aaaatcatca cgctgagtat cggaaagcag cgccgctacc acaccaacgg
     9001 tgcgcgaggc attctgcaca tacaccgtag ggccgctgta gttctgctcg attttcaccg
     9061 ccgtgtgcgc ttttgaggtg gtcgcgccgc caatcagtaa cggaatggtg aagccctgac
     9121 gctccatctc tttcgccacg ttaaccatct cgtccagcga cggcgtgata agccccgaaa
     9181 ggccaatcaa atcagcattc acttctttgg cgatacggag aattttttcc gcaggcacca
     9241 taacgccgag atcgacaatt tcgtagttgt tacattgcag caccacacca acgatatttt
     9301 taccgatgtc gtggacatcg cctttcacgg tggcgatcac catcttgccg ttggttttac
     9361 cctgctcttt gctggcttca ataaacggtt cgagatacgc cactgcctgt ttcatgacgc
     9421 gcgccgattt gaccacctgt ggcaggaaca ttttgccttc gccaaacaga tcgccgacca
     9481 cgttcatgcc gtccatcaac gggccttcaa tcacttcaat cgggcgcgta gcctgctggc
     9541 gggcttcttc agtatcctgc tcgataaact cggttatgcc tttgaccagc gagtattcca
     9601 gacgtttatt cacgtcccac gagcgccact ccgcttgctg ggcgttagca gtgtcgtccg
     9661 ctttactgcc gcgatatttc tcggcaagct ccagtaaacg ctcagtgcca tcgtcacgac
     9721 gattaagaat cacatcttcc accgcgtcgc gcagttcagc gggtaggtcg tcgtaaatcg
     9781 ccagttgccc ggcgttgacg atccccatat ccatgccatt gcgaatagcg tagtagagga
     9841 acactgcgtg aatggcttcg cgcaccggat cgttgccacg gaatgagaaa gaaacgttag
     9901 atacgccgcc ggaaatcagc gcgtgcggca gttcgcgttt gatgtcttca cacgcgccga
     9961 taaagtcctg cgcgtagttg ttgtgctctt caataccggt ggcgaccgcg aagatgttcg
    10021 ggtcgaagat gatatcttct ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc
    10081 gacggcaaat ctcgattttc cgtgcgcgag tatcggcctg tccctgttcg tcaaaggcca
    10141 tcaccaccac tgccgcaccg tagcgacgca acaatttcgc gtgatggata aaggcgtcga
    10201 cgccctcttt catcgagata gagttaacaa tgcctttgcc ctggatacac ttcagacctt
    10261 tttcaatgac atcccatttt gaggagtcga tcataatcgg cacgcgagcg atatccggtt
    10321 caccggcaat cagattgaga aaacgcacca tcgccgcttc ggcatcgagc atcccttcat
    10381 ccatgttgat atcgataatc tgcgcgccgt tttccacctg ctgacgcgcc acatccagcg
    10441 cctcgctgta tttctcttct ttgatcaggc gcttgaactt agcagagccg gtgacgttgg
    10501 tgcgttcacc cacgttcaca aacaggctat catcgccaat gttcagcggc tccaggccag
    10561 acaaacggca ggctacggga atttccggca gtttgcgcgg cgctaatcct tctactgcac
    10621 gactcatcgc tgcaatatgt tgcggcatgg tcccgcagca accaccgacg atattgagaa
    10681 agcccgcttg cgcccattca cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtatt
    10741 caccaaaggc gttaggcagc ccggcgttcg ggtgggcggt gacgtagcat tccgcaatcc
    10801 gtgatagttc ctgcacgtac tggcgcagtt catcgggccc cagcgcacag ttcaggccaa
    10861 aggtcagcgc ttcggcgtgg cgcaatgagt tgtaaaatgc ttcggtggtc tgcccggaga
    10921 gcgtgcgccc ggaggcgtcg gtgatggtgc cggagatcat aatcggcagc tcaacgccca
    10981 gcgcttcaaa ctccgttttc accgcaaaga ccgccgcttt ggcgttaagg gtgtcgaaaa
    11041 cggtttcaat caggatcaga tccgcaccac cttccaccag cgctttggtg gattcgcgat
    11101 aggccgccac cagctggtca aaagtgatgt tgcggaatgc cggatcgttg acgtccgaag
    11161 aaatagacgc cgtgcggttg gttgggccga gaacaccggc aacgtagcgc ggtttctccg
    11221 gcgtgcgcgc ggtccactcg tcagcacggg cgcgcgccag tttcgccgcc gcaaagttga
    11281 tttccgccga cagggattcc atctggtaat ccaccatcgc aatggtcgtg gagttgaagg
    11341 tgttggtttc gatgatatcc gcgcccgctt caaagtaggc gttgtggata gcggcgatca
    11401 cttccggttt actgagtacc agcaggtcgt tgttgccttt gaggtcgcat ggccagtcgg
    11461 caaagcgttc accacgaaaa tcggcttcgt tcagtcgata gctctggatc atggtgccca
    11521 taccgccgtc caacaccaga atacgttcat ttaactgcgc acgcagttgt tccactttgc
    11581 tgctcacact tgctcccgac acgctcaacc cagatttaat aaaaattcaa caaaccatac
    11641 tggcataaac gcatctgtgg taaaagcgac caccacgcaa catgagattt gttcaacatt
    11701 aactcatcgg atcagttcag caactattgc attagctaac aataaaaatg aaaataattt
    11761 ccatgataca gaaaaaggag gctgtcatgg tcgcccccat tcccgcgaaa cgcggcagaa
    11821 aacccgccgt tgccaccgca ccagcgactg gacaggttca gtctttaacg cgtggcctga
    11881 aattactgga gtggattgcc gaatccaatg gcagcgtggc actcacagag ctggcgcaac
    11941 aagccgggtt acccaattcc acgacccacc gcctgctaac cacgatgcaa cagcagggtt
    12001 tcgtgcgtca ggtcggcgaa ctgggacact gggcaatcgg cgcacatgcc tttatggtcg
    12061 gcagcagctt tctccagagc cgtaatttgt tagcaattgt tcaccctatc ctgcgcaatc
    12121 taatggaaga gtctggcgaa acggtcaata tggcggtgct tgatcaaagc gatcacgaag
    12181 cgattattat cgaccaggta cagtgtaccc acctgatgcg aatgtccgcg cctatcggcg
    12241 gtaaattgcc gatgcacgct tccggtgcgg gtaaagcctt tttagcccaa ctgagcgaag
    12301 aacaggtgac taagctgctg caccgcaaag ggttacatgc ctatacccac gcaactctgg
    12361 tgtctcctgt gcatttaaaa gaagatctcg cccaaacgcg caaacggggt tattcatttg
    12421 acgatgagga acatgcgctg gggctacgtt gccttgcagc gtgtattttc gatgagcacc
    12481 gcgaaccgtt tgccgcaatt tctatttccg gaccgatttc acgtattacc gatgatcgcg
    12541 tgaccgagtt tggcgcgatg gtgattaaag cggcgaagga agtgacgctg gcgtacggtg
    12601 gaatgcgctg actttttctg gcgggcagag gcaatattct gcccatcata cctgagtggc
    12661 aatagaataa gggtgtctgt taatcgcatt gacgccaaaa taacttaatg tcatacactt
    12721 cacacacttt tattaactca gcattatttt taaacatcaa accacttaat tattaacaaa
    12781 atcgcaagat ctcattccac cgaaactttt acggctgcat tatgagattg agcgatttat
    12841 gatatttttt tagttgtaat catattaaat tttttcactg ttctgatagt taaaattcaa
    12901 gacatcaata aacaatgaga tatttaaatg attactcgta ttcctcgtag ttctttctct
    12961 gcaaatatta ataatacagc ccagacaaat gaacaccaaa ccctgagtga attgttttat
    13021 aaagaactcg aggataaatt ttctggcaag gagctggcga cgcctctatt aaaaagcttc
    13081 tcagagaact gtagacaaaa tgttcgtcat atctttagca acaaggattt tgtcattaaa
    13141 ttttccacgt ccgtcttaca agctgataag aaagaaatta cgataattaa taaaaacgaa
    13201 aacacgacac tcactcaaac cattgctaca atatttgaag aatacctaat tgaaatttta
    13261 cctcaacgct cagacactct tgataaacaa gaattaaacc taaaatcaga tagaaaagaa
    13321 aaagaattcc caagaattaa acttaatggt caatgttatt ttccggggcg ccccaaaacc
    13381 gtatagtata ccgacacatt gctgcacaat atattaatga tatttatcag aatgttgatt
    13441 acaaacccca tcaagatgat tactcttcag ctgaaaaatt tctcactcac ttcaacaaaa
    13501 aatgcaaaaa ccagactttg gcgttgattt ccagccgtcc tgaggggcgt tgcgttgctg
    13561 cctgcggtga tttcgggcta gttatgaaag catattttga caagatggaa tcaaatggca
    13621 tcagtgttat ggcagccata ttactggtgg ataaccatgc tttgacggtc cggctaagaa
    13681 taaagaacac aactgaagga tgtacccatt acgtgatttc ggtttatgat cctaatgtaa
    13741 ctaacgataa aataagaatt atgagcgaaa gcaaagagga tattaaacac tattctctga
    13801 tggattttat gaatgtagat tatagcctcc tgaaatggtc aaatgatcat gttattaacc
    13861 aatctgttgc aataattcca gcacttccga aagaacagct attgatgtta aaaggaactg
    13921 tggatgaaat aacccctcca ttatcaccag caacgatgaa tttgctaatg gcaattggtc
    13981 agaatcacca acttacgcaa ctgatgattc agctccagaa aatgccagaa ctacatagaa
    14041 cagaaatgtt gactgcctat aatagtatta acctccccgg tttatatttg gctataaatt
    14101 atggcaatgc ggatatcgtt gagactattt tcaattcatt gtcagaaaca ggatatgaag
    14161 ggttactctc gaaaaaaaat ctcatgcata ttctggaggc aaaagataaa aatggttttt
    14221 ctggattatt tttagcgata tcacgtaagg ataaaaatgt tgtaacctcg attctgaacg
    14281 tcttacctaa actggccgca acacatcatt tagataatga acaggtgtat aaattcctga
    14341 gtgccaaaaa tagaacgtcc agccatgttt tataccatgt tatggcgaat ggtgatgccg
    14401 acatgctgaa aattgttttg gtcgcgttac ctttgttaat tcgcacatgt catttgacta
    14461 aagaacaggt actcgatctc ctgaaggcaa aggattttta tggttgccca agactatacc
    14521 tggcgatgca aaatggacat agctatatcg tgaaagttat tctcgaagca ttgccctgtc
    14581 tggcccagga aattaacatt tcagcttccg atattgtaga tcttctgacc gctaaaagtc
    14641 ttgcgcgcga cacgggtttg tttatggcca tgcagcgcgg acatatgaac gttattaata
    14701 ctatttttaa cgcattaccc actcttttta atacgtttaa attcgataaa aaaatatgaa
    14761 gcccctcctc ctggcaaata attctaatga atatcccggt ttgttttcag cgatacagca
    14821 taaacaacaa aacgttgtag agacggttta tcttgcttta tctgaccatg cacgcctgtt
    14881 tggatttacc gctgaagata ttatggattt ttggcaacac aaagcgccac aaaaatactc
    14941 tgcctttgag ttggcttttg aatttggtca ccgggttatt gctgaattaa tccttaatac
    15001 attaaataag atggctgaaa gctttggctt tacggataac cctcgataca ttgcggagaa
    15061 aaattatatg gaagctttac tcaaaaaagc atctccccat accgtacgct aaatctttgc
    15121 ctgcgccgat acgcataaac atcttccaca tgcccttcac gtatgcggtt ttgtagtgcg
    15181 cgccagtaat cagcgcggaa caggtcggcg tgcatctctt caaacagcgg accaatacgc
    15241 gggtcggcgc atagccagtg gcgaaactct tccgggaaaa catcgcccgg cgagacgctg
    15301 taccacggtt cgctggcaag ttcgtcttcc ggatagcgcg gcggcgggat gtcgcggaaa
    15361 ttcacttccg tcatgtagca aatttcatcg taatcataaa aaaccacacg cccgtgacgg
    15421 gtgacaccga agtttttaaa cagcatgtcg ccagggaaaa tgttagcagc ggcaagctgg
    15481 cgaatagcgt taccgtattc ttcaatggcg tcgcgcaact gctgaccttc cacttgttcc
    15541 agccagatat tgagcggcac catccgccgc tcgatataga gatggcgaat tacaatctgc
    15601 tcaccaagat cggtgatttt ttccgctgct tcctggagca gtaattccat taataccggg
    15661 gaaatatgcc gcttctccag cacaaagttt tcaaactcct gggtgtccgc cattcggccc
    15721 acacggtcgt gctctttcac cagttgatag caggcacgaa cgtgagcggc agacatctct
    15781 ttctgcggcg cgaacttgtc tttgatgact ttgaataccc gatcaaagcc cggcagcgta
    15841 aacaccaaca tcaccattcc acgaataccc ggcgcttcaa tgaactgctc attacagccc
    15901 tgtagataaa cgagatattc gcggtagctt tcggttttgg cgtgcttctg gcagccgata
    15961 gccatataca attcagcagt ggttttacct ggcagaattt cccgtagcca ctcgaccagc
    16021 gctgcgggca gcggtgcata aaccataaaa taagaacgcg caaagccaaa aacaatgctc
    16081 gcttcggcgg tcgtcgtcag gcaggtatca ataaataact cgccgtcgtc cgtctggtgg
    16141 atcggcagta agaagggcaa cgtgccggaa ggcgtgatca gtttgcccac cagccaggcg
    16201 gctttattgc ggtaaaacag ttcgttcgcc acctgtaaat gactttccgc gaggttgtct
    16261 gtccccagcg tttccgtcag atggcgaatg atgtaatgaa tgtcacggct tttattctgc
    16321 cagcgcaggc gcagcggcag gtcgctgata acgcgcatca gtagagattc ccagccgtga
    16381 tcggggtgaa agtctttcgc cagcgggcgg ggaatggtac gaaagcggcg ctctggctga
    16441 gagctaaaga taaaaagccg ctcgggagta agcgagcggt ggtcaaataa ccgacagtac
    16501 acggagttaa aaaagctctc cgcaatctcg aagcgcgggt aatccggcaa cagccgcgtg
    16561 taatgctctt taacgcgcag taaaaatgcc gcgtccgtac tttggccgtt agtaatgcag
    16621 cgcagttgct ccacgaccag accaacgtga tgatcgtaaa gatggatacg gtttttcatc
    16681 gcctgctgga cagcatgcca gtcggcctgt tcgaaacgct gctgcgcccc ggaagtcact
    16741 tcgaggaatc gaccatactg agcgtcgaag ccttgcaaaa tggtttgagc aatcagtaat
    16801 tccaggccac gcggcattta gcgcactcat caggagcaga gaattgccgg atgcggcgta
    16861 aacaccttat ccagcctacg ttcggcaacg gttgtaggca tgataagacg cgcaagcgtc
    16921 gcatcaggca tcgtgcaccg attgccggat gcggcgtaaa tgccttatcc ggcctacagt
    16981 cagcaacgtt tgtaggttgc ttagaactgc gattcttcag tggagccagt cagcgcggtg
    17041 actgaagacg tgccgccctg aataatagtc gtcactttat cgaagtaacc tgtacccact
    17101 tcctgctggt gagatacgaa ggtatagcca tctttcgcgg cggcaaattc cggctgctgc
    17161 actttctcaa catagtgctt catactttcg ccctgcgcat aagcgtttgc caggtcgaac
    17221 atgttaaacc acatgctgtg gatacctgcc agggtgatga actggaactt gtagcccata
    17281 tccgacaact gctgctggaa gctggcaata gttttgtcgt cgaggttttt ctgccagttg
    17341 aacgacggcg agcagttata agccagcagt ttgcccggat atttcgcgtg gatagcttgt
    17401 gcaaagcgac gcgccagttc cagatccggc gtggaggttt cacaccagac caggtcagca
    17461 tatggcgcat acgccaggcc acggctgatc gcttgctcaa tgcccgcatg agtacggaag
    17521 aagccttcac tggtacgctc gccggtaata aattcgctgt catacgggtc gcaatctgag
    17581 gtgatcagat ccgccgcatc agcatcggta cgtgcaacca gcagggttgg aacgcccgtc
    17641 acgtcagctg ccagacgcgc cgcgaccagt ttctgaatag cttcctgagt tggcactaaa
    17701 actttgccgc ccatgtgacc gcatttcttc actgacgcca gctgatcttc gaagtgaact
    17761 gccgcagcac cggcttcaat catcgctttc atcagttcaa aggcattcag gacaccgcca
    17821 aaaccggctt ccgcatcggc aacgatcggc aggaagtaat cgacatagcg cggatcgccc
    17881 ggctcaatgc ccacggacca ttggatctga tcggcacgac ggaaggtgtt gttgatccgc
    17941 tccaccacag ctggcaccga gtttgccgga tagagcgact gatccggata catgctggcc
    18001 gccaggttag cgtccgccgc tacctgccat cccgacagat agactgcttc aatacccgct
    18061 ttcgcctgtt gcagcgcctg accgccagtc agtgcaccga ggctgttgat gtagcctttt
    18121 ttcgactcac cgtgcagcag acgccacatt ttcgctgcgc ccagttgcgc cagcgtgcat
    18181 tcaggattga ctgaaccgcg taatttcacc acatcttccg cactgtatgg gcgagtaatg
    18241 ccttcccaac gcggttgagt ccactctttc tgtaattctt caatttgttg tgtacgggtt
    18301 ttcatgtgca gatgctccat agttatgtgg tggtttacgc taacaggcgg tagcctggca
    18361 gggtcaggaa atcaattaac tcatcggaag tggtgatctg ttccatcaag cgtgcggcat
    18421 cgtcaaaacg gccctgggag aaacgttctt cgcccagttc gctggcaatg actttcatct
    18481 cttcgcccag catctggcgg aacaaggctt tggtcaccgg tttgccattg ctcaacgttt
    18541 tttgatgatg gatccactgc cagatcgagg tacgggaaat ttcagccgtc gccgcatctt
    18601 ccatcaggcc ataaatcggc acgcagccgt tgccggagat ccacgcttcg atgtactgca
    18661 cagccacgcg aatgttggcg cgcatacctt cttcggtgcg ttcaccatca caaggtgcca
    18721 gcagctgatc ggcagtaatc ggcgcgtctt gttcgcgcaa tacttcaagc tgatttttac
    18781 gggagccgag aatatcgttg aataccgcca ttgccgtgtc cgcaaggcct gggtgagcga
    18841 tccatgtgcc atcgtgaccg ttattggctt ccagcgcttt atccgctttt actttgttga
    18901 gcacctggtt attacgctct tcatctttgc tcggaataaa cgctgccatg ccgcccatcg
    18961 caaaagcacc gcgtttatgg caggttttaa tcagcaggcg tgagtaagca ttcaggaatg
    19021 gtttatccat cgtcacggcc tgtctgtctg gcaggacacg atcgggatag tttttcaacg
    19081 ttttgatata gctgaagatg taatcccagc gaccgcagtt cagaccaaca atatggtcgc
    19141 gcagcgcgtg aaggatttcg tccatctgga acaccgcggg cagcgtttca atcagcaacg
    19201 tcgccttgat ggtgccgcgc ggcagattaa agcgatcttc tgcatagctg aagacttcgc
    19261 tccaccaggc cgcttcctgc caggactggg ttttcggcag atagaaatag ggaccgcagc
    19321 cctttgccaa cagtgcctga tagttgtgga agaaatagag cgcaaaatca aacaggctac
    19381 cggggattgc ctcaccacgc caggtgacat gtttttccgg caagtgcaga ccgcgtaccc
    19441 gacaaatcaa aaccgctgga ttgggcttga gctgataaat tttgcctgct tcattggtat
    19501 agctgatggt accgttaacc gcatcgcgca ggttaatttg cccgtcgatc actttgttcc
    19561 agtctggtgc cagtgaatct tcgaaatcgg ccataaagac tttcacattg gcgttcagtg
    19621 cgttgatcac catcttgcgc tctaccgggc cagttatctc tacgcggcgg tcttctaagt
    19681 ccgcaggaat cccgcgaatt ttccaatcag catcgcgaat ggaagccgtt tccgaaataa
    19741 aatcaggcaa cgttccgtta tcaatatctt gctgctgctg aatgcgcgct gccagaagtt
    19801 tattgcgttg tggcgtaaaa tgcgtcacca gctcagtcag aaattctacc gcttcggcag
    19861 taagaatttg cttctcctgc tcgccatacg gccttgtgaa agccagttca tcggttgttg
    19921 ttgcctgttc agtcatcgtg cagctcctcg tcatggatcc gaaaacttcc cctctgtgaa
    19981 cgaaggaatg ttgtgcgctt ttcgttcagc acaactaaga ctactcattt aaaatgcaaa
    20041 aacaaaaaca atttccattt taattaaaaa tacttgataa cttattgata acaatatgaa
    20101 taaaatttat tcactttctg aggtgcgttt cggaaataaa aaaggcaccc gaaggtgcct
    20161 gaggtaaggt gctgaatcgc ttaacgatcg actatcacag aagattaatc cagcgttgga
    20221 ttcatgtgcc gtagatcgta tggcgtgatc tgatagacgt aatagttgag ccagttggta
    20281 aacagcaaat taccgtgact acgccagctc gctcgcggtt tgttttgcgg atcgttgtgc
    20341 gggaaatagt tatacggtac atccgggtct agtccggctt ccacatcgcg gaaatattcc
    20401 tgcgccagcg tttgcgcatc atattcggga tggcccgtca caaaggcaat gcgcttatct
    20461 ttgctggcaa acagatatgc atccccttct tccgtctctg ccagaatttc cagatcggtg
    20521 taatcacgaa tcaacgctgc cggaaagtca gcatagcgcg aatgcggtgc caggaatgaa
    20581 tcatcaaagc cacgcgtcag aagcgcatga ggatggagaa tatgatgctc gtaaacgcca
    20641 gagagtttgt cggtgcgagt ttgcttagga atgccgtaga ggatattgag cgcggcctgt
    20701 accgcccagc agacaaacag cgtcgaggtg acgtgatctt tcgaccactc cagcacctgt
    20761 ttgatctgcg gccagtaagc gacatcatta aactccacca ggcccagcgg cgcaccggtt
    20821 acaatcagac cgtcaaagtt ctgttcctga atatcttcaa agttacagta gaagttgttc
    20881 agatgctctg cgggcgtgtt gcgcgattca cgggaatcga tgcgcaacag ctgaatatcg
    20941 acctgcaaag gtgagtttga aagcaggcgc agaaactgat tttcagtttc aatcttcttc
    21001 ggcatcaggt taaggatcag aaccttaagt ggacgaattt cctgaccaga cgcacgagaa
    21061 gttgtcatca caaacacgtt ttcttcacgc aagaaattga cggcgggtag ctcgtccggc
    21121 acacgaatcg gcataacctg attacctcac tacatacgct tatacgttta gacatccaga
    21181 tagctgaaga taaccagaaa atttgccaat gtcgagcctg catgttgaag gtgagaaagt
    21241 ttcaaggaaa aggctacgtt agaatataag aatgacgaaa aggagagaaa atggttatta
    21301 gtattcgccg ctcacggcat gaggaagggg aggaactcgt tgcgatttgg tgtcgatctg
    21361 tcgatgccac ccacgatttt ttatcagcag agtatcgggc cgagctggaa gagctggtgc
    21421 gttcctttct gccggaagca ccgttgtggg tcgcggttaa tgaacgggag cagccggttg
    21481 ggtctatgtt gttaagtggg cagcatatgg atgcgctgtt tatcgatcct gatgtgtgcg
    21541 gctgcggcgt gggtcggatg ctggtggagc atgcgctctc gatggcaccg gaactgacaa
    21601 ccaacgttaa tgagcaaaat gagcaggcgg ttgggttcta taagaaggtg ggttttaagg
    21661 ttacgggacg ctctgaggtg gacgatttgg ggaaaccgta tccgttgctg aatctggcgt
    21721 atgtggggga taagccgccc ctgccgtgcc tgcccaagct ggagaagacg cgccagcgtc
    21781 gcacccggca tttttttcac caacgcaaaa aggccatcct gacggatagc ctttcgctta
    21841 atttgatgcc tggcagttcc ctactctcgc atggggagac cccacactac catcggcgct
    21901 acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc tacggccgcc
    21961 aggctaattc tgttttatca gaccgcttct gcgttctgat ttaa
//
LOCUS       DAJVQH010000062        21518 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJVQH010000062 DAJVQH010000000
VERSION     DAJVQH010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.062"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OQM87_002365"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OQM87_002365"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCS1508473.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OQM87_002366"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OQM87_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCS1508474.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLHD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OQM87_002367"
     CDS             complement(2649..3200)
                     /locus_tag="OQM87_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCS1508475.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OQM87_002368"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OQM87_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCS1508476.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="OQM87_002369"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="OQM87_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCS1508477.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OQM87_002370"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OQM87_002370"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCS1508478.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OQM87_002371"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OQM87_002371"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCS1508479.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OQM87_002372"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OQM87_002372"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCS1508480.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OQM87_002373"
                     /pseudo
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OQM87_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OQM87_002374"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OQM87_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCS1508481.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="OQM87_002375"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="OQM87_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCS1508482.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="OQM87_002376"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="OQM87_002376"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCS1508483.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OQM87_002377"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OQM87_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCS1508484.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OQM87_002378"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OQM87_002378"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCS1508485.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OQM87_002379"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OQM87_002379"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCS1508486.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSGNTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OQM87_002380"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OQM87_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCS1508487.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="OQM87_002381"
     CDS             complement(18174..18569)
                     /locus_tag="OQM87_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1508488.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OQM87_002382"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OQM87_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1508489.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="OQM87_002383"
     CDS             19485..20078
                     /locus_tag="OQM87_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1508490.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /gene="tabA"
                     /locus_tag="OQM87_002384"
     CDS             20223..20675
                     /gene="tabA"
                     /locus_tag="OQM87_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCS1508491.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20798..>21518
                     /locus_tag="OQM87_002385"
     CDS             20798..>21518
                     /locus_tag="OQM87_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1508492.1"
                     /translation="MSKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKALN"
BASE COUNT         5203 a         5330 c         5662 g         5323 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccaccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcacgaccg
     1501 ctgggtgcta gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggctcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 ctttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgtcac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccaacgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgt atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg ggaggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gctatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acacaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctccgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg atcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tgccgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg ttcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac gcctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttaccggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtt actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcggggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacattg ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag cacgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca
    20701 ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt
    20761 aatgcttaga cgttcttatt aactcaagga gttcgtcatg agcaaaatat cagatttgaa
    20821 ttattctcaa cacattacat tagccgacaa ttttaaacaa aaaaatgaag ccttagatac
    20881 ctggtatgtg ggaatgaatg attttgcccg aattgccggg gggcagaata gcagaagcaa
    20941 tattctttct cctagagcat ttttggagtt tttggcaaag atatttaccc taggttatgt
    21001 ggattttagc aaacgctcca acgaagcggg tagaaatatg atggctcata ttgagttctc
    21061 atcttattct aaagatactg atggcaatga aaaaatgaag ttttacatga ataatcctga
    21121 aggggagcga gcggatttat ctaaggtgaa gatagaaatt acacttgcct cagcttctac
    21181 taaagggatt cgtgaaggac atacagtcat tatatttaaa caatctgatg gttcgactaa
    21241 tcgttatgaa gggaagtctt ttgaaagaaa agatgatagt tcattacacc tgattactaa
    21301 caaggttctg gcgtgttacc aacgcgaagc taacaagaaa atagcgcggc tattaaataa
    21361 tcatcagaag ttaaataata tacaggagtt aaatgattcg caggagttaa ataattcaca
    21421 gaagttaaat aattcgcagg agttaaataa ctcgcaggtg agttgtaaag gttcagttga
    21481 ttctacgatt acggatttat tagaaaaagc attgaata
//
LOCUS       DAJVQH010000063        21303 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJVQH010000063 DAJVQH010000000
VERSION     DAJVQH010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.063"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OQM87_002386"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OQM87_002386"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCS1508493.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OQM87_002387"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OQM87_002387"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCS1508494.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="OQM87_002388"
     CDS             complement(3121..3420)
                     /locus_tag="OQM87_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCS1508495.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="OQM87_002389"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="OQM87_002389"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCS1508496.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="OQM87_002390"
     CDS             complement(4632..5069)
                     /locus_tag="OQM87_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCS1508497.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="OQM87_002391"
     CDS             complement(5115..5456)
                     /locus_tag="OQM87_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508498.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="OQM87_002392"
     CDS             complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="OQM87_002392"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCS1508499.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="OQM87_002393"
                     /pseudo
     CDS             complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="OQM87_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8838..9830
                     /gene="xylF"
                     /locus_tag="OQM87_002394"
     CDS             8838..9830
                     /gene="xylF"
                     /locus_tag="OQM87_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1508500.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEREL"
     gene            9908..11449
                     /gene="xylG"
                     /locus_tag="OQM87_002395"
     CDS             9908..11449
                     /gene="xylG"
                     /locus_tag="OQM87_002395"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCS1508501.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11427..12608
                     /gene="xylH"
                     /locus_tag="OQM87_002396"
     CDS             11427..12608
                     /gene="xylH"
                     /locus_tag="OQM87_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCS1508502.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12686..13216
                     /locus_tag="OQM87_002397"
     CDS             12686..13216
                     /locus_tag="OQM87_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1508503.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13328..13864
                     /locus_tag="OQM87_002398"
     CDS             13328..13864
                     /locus_tag="OQM87_002398"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1508504.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGTRQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13972..14796)
                     /locus_tag="OQM87_002399"
     CDS             complement(13972..14796)
                     /locus_tag="OQM87_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCS1508505.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15116..17146
                     /gene="malS"
                     /locus_tag="OQM87_002400"
                     /pseudo
     CDS             15116..17146
                     /gene="malS"
                     /locus_tag="OQM87_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="OQM87_002401"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="OQM87_002401"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCS1508506.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGETELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="OQM87_002402"
     CDS             complement(18728..19201)
                     /locus_tag="OQM87_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1508507.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="OQM87_002403"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="OQM87_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCS1508508.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            20352..>21303
                     /gene="yiaK"
                     /locus_tag="OQM87_002404"
     CDS             20352..>21303
                     /gene="yiaK"
                     /locus_tag="OQM87_002404"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCS1508509.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRN"
BASE COUNT         5356 a         5436 c         5227 g         5284 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 cccccgtacg gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact
     5881 cactacgcgc cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca
     5941 cgacatccat gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt
     6001 cattggggcc atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt
     6061 gtggtgtacg ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag
     6121 cctgttgagc tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcggccc
     6181 aatccagaca cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg
     6241 catggcaaaa gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa
     6301 aatagacccc cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac
     6361 caactgcacc agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc
     6421 acgctttcgc aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcgg
     6481 gcatctggtc acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg
     6541 cgacatccag ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca
     6601 tacgcagacg caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg
     6661 gctcatgccg ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc
     6721 cggtaatcac tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc
     6781 gcccgtcgtt ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg
     6841 ctccatgcat ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc
     6901 ccagagcttt cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc
     6961 aaagtggatg cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct
     7021 gctcgttgag caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat
     7081 cgatcgttcc ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac
     7141 agttagccgt tatttgtcga acagataatg atttaccaga ttttccagtt gctcctggcg
     7201 accactctga tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag
     7261 tgacatttgg cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg
     7321 cgcgatgcgt ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag
     7381 tgccatcgta tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg
     7441 acgacgtact ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat
     7501 ttcatacatc accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc
     7561 cagttgcgca tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt
     7621 ggctatttca tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag
     7681 tttaatctct ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta
     7741 atcatattga tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctagaa
     7801 gccgatttta tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc
     7861 ctgacgcaag tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata
     7921 gttttcaccg cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca
     7981 gctgaagact tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa
     8041 gcagttagcg gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac
     8101 atcaaccatt tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac
     8161 atccacatcg tggaagcaat aaaatggcac atgtaacttg tggaaaaact caaatgcgac
     8221 atctgcttta cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa
     8281 cgcccccaca ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt agcaggcggc
     8341 aaaacgcaag tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg
     8401 gaatgctaac gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa
     8461 ataggcttgc atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta
     8521 atattgggca ctccctttca attgctcaat tatgttattt cacactgcta ttgagataat
     8581 tcacaagtgt gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga
     8641 agataaaaat gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag
     8701 aaataaacca aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt
     8761 tgctaaaaat tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact
     8821 acagaaggcc ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc
     8881 tgcttaccaa cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc
     8941 tccgtcttga acgctggcaa aaagatcgag atatttttgt gaaaaaggca gaatctctcg
     9001 gtgcgaaagt atttgtacag tctgcaaatg gcaatgaaga aacacaaatg tcgcagattg
     9061 aaaacatgat taaccggggt gtcgatgttc ttgtcattat tccgtataac ggtcaggtat
     9121 taagtaacgt tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta
     9181 tgattaacga tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac
     9241 tgcaggcaaa agccttggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct
     9301 cgccggtaga taacaacgcc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt
     9361 acgttgattc tggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg
     9421 aaaacgcatt gaaaattatg gaaaacgcgc taaccgccaa taataacaaa attgatgctg
     9481 tagttgcctc aaacgatgcc accgcaggtg gggcaattca ggcattaagc gcgcaaggtt
     9541 tatcagggaa agtagcaatt tccggccagg atgcggatct cgcaggtatt aaacgtattg
     9601 ctgccggtac gcaaactatg acggtgtata aacctattac attgctggca aatactgccg
     9661 cagaaattgc cgttgaattg ggcaatggtc aggagccaaa agcagatacc tcactgaata
     9721 atggcctgaa agatgtcccc tcccgcctcc tgacaccgat cgatgtgaat aaaaacaaca
     9781 tcaaagatac ggtaattaaa gacggattcc acaaagagag agagctgtaa gcgttacgcc
     9841 ccggcgcgga gcgggggcgt gatttctctc catgccgcgt gaatgaattg gcttaggtgg
     9901 agtcgttatg ccttatctac ttgaaatgaa gaacattacc aaaaccttcg gcagtgtgaa
     9961 ggcgattgat aacgtcagct tgcggttgaa tgctggcgaa atcgtctcac tctgtgggga
    10021 aaatgggtct ggtaaatcaa cgctgatgaa agtgctgtgt ggtatttatc cccatggctc
    10081 ctacgaaggc gaaattattt ttgcgggaga agagattcag gcgagtcaca tccgcgatac
    10141 cgaacgcaaa ggtatcgcca tcattcacca ggaattggcc ctggtgaaag aattgaccgt
    10201 gctggaaaat atcttcctgg gtaacgaaat aacccacaat ggcattatgg attatgacct
    10261 gatgacgcta cgctatcaga agctgctcgc acaggtcagt ttatccattt cacctgatac
    10321 ccgcgttggc gatttagggc ttgggcaaca acaactggtt gaaattgcca aggcacttaa
    10381 taaacaggtg cgcttgttaa ttctcgatga accgacagcc tcattaactg agcaggaaac
    10441 gtcggtttta ctggatatta ttcgcgatct acaacagcac ggtatcgcct gtatttatat
    10501 ttcgcacaaa ctcaacgaag tcaaagcgat ttccgatacg atttgcgtta ttcgcgacgg
    10561 acagcacatt ggtacgcgtg atgctgccgg aatgagtgaa gacgatatta tcaccatgat
    10621 ggtcgggcga gagttaaccg cgctttaccc taatgaacca cataccaccg gagatgaaat
    10681 attacgtatt gaacatctga cggcatggca tccggtcaat cgtcatatta aacgagttaa
    10741 tgatgtctcg ttttccctga aacgtggcga aatactgggt attgccggac tcgttggtgc
    10801 cggacgtacc gagaccattc agtgcctgtt tggcgtgtgg cccggacaat gggaaggaaa
    10861 aatttatatt gatggcaaac aggtagatat tcgtaactgt cagcaagcca tcgcccaggg
    10921 gattgcgatg gtacccgaag acagaaagcg cgacggcatc gttccggtaa tggcggttgg
    10981 taaaaatatt accctcgccg cactcaataa atttaccggt ggcattagcc agcttgatga
    11041 cgcggcagag caaaaatgta ttctggaatc aattcagcaa ctcaaagtta aaacgtcgtc
    11101 ccccgacctt gctattggac gtttgagcgg cggcaatcag caaaaagcga tcctcgcccg
    11161 ctgtctgtta cttaacccgc gcatcctcat tcttgatgaa cctaccaggg gtattgatat
    11221 tggcgcgaaa tatgagatct acaaattaat taaccaactc gtccagcagg gtattgccgt
    11281 tattgtcatc tcttccgaat tacctgaagt gctcggcctt agcgatcgtg tactggtgat
    11341 gcatgaaggg aaactaaaag ccaacctgat aaatcataac ctgactcagg agcaggtgat
    11401 ggaagccgca ttgaggagcg aacatcatgt cgaaaagcaa tccgtctgaa gtgaaattgg
    11461 ccgtaccgac atccggtggc ttttctgggc tgaaatcact gaatttgcag gtcttcgtga
    11521 tgattgcagc tatcatcgca atcatgctgt tctttacctg gaccaccgat ggtgcctact
    11581 taagcgcccg taacgtctcc aacctgttac gccagaccgc gattaccggc atcctcgcgg
    11641 taggaatggt gttcgtcata atttctgctg aaatcgacct ttccgtcggc tcaatgatgg
    11701 ggctattagg tggcgtcgcg gcgatttgtg acgtctggtt aggctggcct ttgccactta
    11761 ccatcattgt gacgttggtt ctgggactgc ttctcggtgc ctggaacgga tggtgggtcg
    11821 cgtaccgcaa agtcccttca tttattgtca ccctcgcggg catgttggca tttcgcggca
    11881 tactcattgg catcaccaac ggcacgactg tatcccccac cagcgccgcg atgtcacaaa
    11941 ttgggcaaag ctatctcccc gccagtaccg gcttcatcat tggcgcgctt ggcttaatgg
    12001 cttttgttgg ttggcaatgg cgcggaagaa tgcgccgtca ggctttgggt ttacagtctc
    12061 cggcctctac cgcagtagtc ggtcgccaag ctttaaccgc tatcatcgta ttaggcgcaa
    12121 tctggctgtt gaatgattac cgtggcgttc ccactcctgt tctgctgctg acgttgctgt
    12181 tactcggcgg aatgtttatg gcaacgcgga cggcatttgg acgacgcatt tatgccatcg
    12241 gcggcaatct ggaagcagca cgtctctccg ggattaacgt tgaacgcacc aaacttgccg
    12301 tgttcgcgat taacggatta atggtagcca tcgccggatt aatccttagt tctagacttg
    12361 gcgctggttc accttctgcg ggaaatatcg ccgaactgga cgcaattgca gcatgcgtga
    12421 ttggcggcac cagcctggct ggcggtgtgg gaagcgttgc cggagcagta atgggggcat
    12481 ttatcatggc ttcactggat aacggcatga gtatgatgga tgtaccgacc ttctggcagt
    12541 atatcgttaa aggtgcgatt ctgttgctgg cagtatggat ggactccgca accaaacgcc
    12601 gttcttgatt ttgataaaaa ttttctcaaa gccggttacg tattaccggt tttgagtttt
    12661 tgcatgattc agcaggaaaa gaaccatgtt tactaaacgt caccgcatca cattactgtt
    12721 caatgccaat aaagcctatg accggcaggt agtagaaggc gtaggggaat atttacaggc
    12781 atcacaatca gaatgggata ttttcattga agaagatttc cgcgcccgca ttgataaaat
    12841 caaggactgg ttaggagatg tcgtcattgc cgacttcgac gacaaacaga tcgagcaagc
    12901 gctggctgat gtcgacgtcc ccattgttgg ggttggcggc tcgtatcacc ttgcagaaag
    12961 ttacccaccc gttcattaca ttgccaccga taactatgcg ctggttgaaa gcgcattttt
    13021 gcatttaaaa gagaaaggcg ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg
    13081 caaacgttgg gccactgaac gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta
    13141 tcgcggagtg gtttatcagg ggttagaaac cgcgccagag aactggcaac acgcgcaaaa
    13201 tcggctggca gactgactac aaacgctgcc accgcaaacc gggattattg ccgttactga
    13261 cgcccgagcg cggcatattc tgcaagtatg tgaacatcta catattcccg taccggaaaa
    13321 attatgcgtg attggcatcg ataacgaaga actgacccgc tatctgtcgc gtgtcgccct
    13381 ttcttcggtc gctcagggca cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg
    13441 attattagat aaagaagaaa tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat
    13501 tgaacggcgc tcaacagatt atcgctcgct gaccgatccc gccgttattc aggccatgca
    13561 ttacattcgt aatcacgcct gtaaagggat taaagtggat caggtactgg atgcggtcgg
    13621 gatctcgcgc tccaatcttg agaagcgttt taaagaagag gtgggtgaaa ccatccatgc
    13681 aatgattcat gccgagaagc tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc
    13741 gatcaatgag atatcgcaaa tgtgcggtta tccatcgctg caatatttct actctgtttt
    13801 taaaaaagca tatgacacaa cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt
    13861 gtagcgatga tgagaattgt cggcgtcaca tcagggaact cagcacagcc acctgatgcg
    13921 agcaaaagcc ttatccgacc gacaaatcag ataaggctcg aggaaatgaa attacatatg
    13981 cgccgcgatt aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt
    14041 ccctttggtc gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc
    14101 cgctttacgc agctgtgcac gcgatttacg gaacgaagag taagccgggt gcgtattcag
    14161 gttagtgaca taggcgctca ccgattcttt gacagaacta aactgtgagt accctttcac
    14221 tttacccggc gcattggtac aacgtccttt catgcatttc atgccgaaca gattgttatt
    14281 gttgcgcgcc agcttcgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat
    14341 actggtaggg ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt
    14401 accagaccac ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg
    14461 tgaccattgg ccctgatact gttttgaaat cagccagtta cgttctgcag taatggctgc
    14521 attttggctg gtaatgtaag gcattacggt ccggagaaac gcctttttcc ttggtgttcc
    14581 ggaagggtat tttcgcaaat caggaagtga actactcttt gcactattgc gagaatactc
    14641 ttgtttactg cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt
    14701 tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat
    14761 catcgcccca tatcgtcgta tgggagtcaa aatcatcagg tctcctggcc ggatttaatc
    14821 attccaacac cttatatttt tcacaaattt gagagttgaa tctcaaatca tatcaaaaat
    14881 agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat
    14941 tcagattggc gctaaatcgc agaaaatgtg ggggttatcg caaaattcag ccgttttttg
    15001 cgcgcgatcg ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg
    15061 tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccaccatgaa
    15121 actcgccgcc tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc
    15181 tccggggttc cctgccttta gcgaacaggg aacgggaaca tttgtcagcc acgcgcagtt
    15241 gcccaaaggt acgcgtccac tcacgctaaa ttttgaccaa cagtgctggc agcctgcaga
    15301 tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt agcaacacgc cgcctcaatg
    15361 gcgattgttc agggacggca aatatacgct gcaaatagac acccgctccg gtacgccaac
    15421 attgatgatt tccatccaga acgccgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccggg caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acacctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctag ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggga aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag cgcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc tgcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagctt gggcagaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgctgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga gcgcgcccag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgtg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagtttcgc atattgaaat tttaagacaa aaaagcgatc aaaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acg
//
LOCUS       DAJVQH010000064        21239 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJVQH010000064 DAJVQH010000000
VERSION     DAJVQH010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.064"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(32..1012)
                     /locus_tag="OQM87_002405"
     CDS             complement(32..1012)
                     /locus_tag="OQM87_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCS1508510.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1020..1730)
                     /locus_tag="OQM87_002406"
     CDS             complement(1020..1730)
                     /locus_tag="OQM87_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCS1508511.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1946..3769)
                     /gene="typA"
                     /locus_tag="OQM87_002407"
     CDS             complement(1946..3769)
                     /gene="typA"
                     /locus_tag="OQM87_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCS1508512.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4142..5551
                     /gene="glnA"
                     /locus_tag="OQM87_002408"
     CDS             4142..5551
                     /gene="glnA"
                     /locus_tag="OQM87_002408"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCS1508513.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5724..6773
                     /gene="glnL"
                     /locus_tag="OQM87_002409"
     CDS             5724..6773
                     /gene="glnL"
                     /locus_tag="OQM87_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCS1508514.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6785..8194
                     /gene="glnG"
                     /locus_tag="OQM87_002410"
     CDS             6785..8194
                     /gene="glnG"
                     /locus_tag="OQM87_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCS1508515.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8306..8416
                     /locus_tag="OQM87_002411"
     CDS             8306..8416
                     /locus_tag="OQM87_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCS1508516.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8645..10018)
                     /gene="hemN"
                     /locus_tag="OQM87_002412"
     CDS             complement(8645..10018)
                     /gene="hemN"
                     /locus_tag="OQM87_002412"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCS1508517.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10207..10716)
                     /gene="yihI"
                     /locus_tag="OQM87_002413"
     CDS             complement(10207..10716)
                     /gene="yihI"
                     /locus_tag="OQM87_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCS1508518.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11298..11930
                     /locus_tag="OQM87_002414"
     CDS             11298..11930
                     /locus_tag="OQM87_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCS1508519.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12312..15098)
                     /gene="polA"
                     /locus_tag="OQM87_002415"
     CDS             complement(12312..15098)
                     /gene="polA"
                     /locus_tag="OQM87_002415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCS1508520.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYTEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15462..16394
                     /locus_tag="OQM87_002416"
     CDS             15462..16394
                     /locus_tag="OQM87_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1508521.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16435..17865)
                     /locus_tag="OQM87_002417"
                     /pseudo
     CDS             complement(16435..17865)
                     /locus_tag="OQM87_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
     gene            complement(18020..18646)
                     /gene="dsbA"
                     /locus_tag="OQM87_002418"
     CDS             complement(18020..18646)
                     /gene="dsbA"
                     /locus_tag="OQM87_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCS1508522.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18663..19649)
                     /gene="srkA"
                     /locus_tag="OQM87_002419"
     CDS             complement(18663..19649)
                     /gene="srkA"
                     /locus_tag="OQM87_002419"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCS1508523.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRQTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19726..19995)
                     /locus_tag="OQM87_002420"
     CDS             complement(19726..19995)
                     /locus_tag="OQM87_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCS1508524.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20065..20649
                     /gene="mobA"
                     /locus_tag="OQM87_002421"
     CDS             20065..20649
                     /gene="mobA"
                     /locus_tag="OQM87_002421"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCS1508525.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20631..21158
                     /gene="mobB"
                     /locus_tag="OQM87_002422"
     CDS             20631..21158
                     /gene="mobB"
                     /locus_tag="OQM87_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCS1508526.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5178 a         5346 c         5120 g         5595 t
ORIGIN      
        1 gttttattaa ctgcgtatga gttaaaactt attagtggtt cagaagatgg tttgcctctt
       61 gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca tcttcttttt
      121 ctttacgtaa ccgcgcatcc agtaaatgat tttctggtag gggtgtttca ctcatctggc
      181 gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat
      241 caacctcttc ttccaggcca tatgccgtca cctgcggctc atcatcacca aggcagataa
      301 ggtgcgttaa taacgctttg aatggcatat caccctgacc tgaaatacag gctttatgac
      361 ccaggccacc ctgttcttta acgatcaagg catctttaat atggacctgg gtaatatgcg
      421 gtgccatcgt ttttaaggcg tcgatgggat gctcatttgc attgatcatg ttagcaaaat
      481 caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc agctcatgac
      541 tttttaaatc ttcatgctgc tcaatcgtaa aagtcaggcc gctgtcctga tacgtttccc
      601 gtacataggc aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta ccttcataac
      661 gtggataaaa acgtacggac gttgccccag ttttcaacgc gatggcgacg gcttcgtcaa
      721 tagatgcctt atctgaagcg ctggtttcaa tatgaatatc aaggttcaga cggcgggctt
      781 tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa cgctcgccat
      841 ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca agtaaatcag
      901 caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt aaataaagag
      961 gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc ataaaacacc
     1021 tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc tatctggaca
     1081 atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat acccgcagaa
     1141 ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat caattttttt
     1201 actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg gttttcaagt
     1261 tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata gaaaacttta
     1321 tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg cgtcactgca
     1381 atcagtggcg cgagcgtctc gggaatatcg ctggtacggt ttttctcggt aaacccccaa
     1441 gaaggctctc gcccttgctc aattgcggcc cgctgaaagc tcgccgacag ctccggactg
     1501 taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa gattttggat
     1561 tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc cagcagttct
     1621 cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc tttcaaccac
     1681 cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat tgtttaatcc
     1741 ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag caattaacgg
     1801 tgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaatcac agacttataa
     1861 gggagatact cgcattggtg tgcgctcacg acaggtaaaa aaaacctgcc atcgctggca
     1921 ggttttttat gactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg gcgcggcgac
     1981 gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc acttctacca
     2041 gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata ggcggaacca
     2101 gaacaacagc ttcgtcagta ccggaagcac gcatgttggt cagtttctta ccggtcaggc
     2161 agtttacagt caggtcgtta gagcggctat gaataccgat aatctgacct tcgtaaactt
     2221 ctgcaccgtg accgaggaac agcttaccgc gatcctgcag accgaacagc gcgaacgcga
     2281 cagctttacc ctgaccgtta gagatcagta caccgttctg acgctgaccc acttcgcccg
     2341 gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg gaagtcatgg
     2401 tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag tcgagacgta
     2461 cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg cccagcgcct
     2521 gcatcacaga accctgatgc tgttcttcaa cgtccagcgt cacgttttca tacggctctt
     2581 gtttacgacc gtcgatttca cggaagatta ctttcggacg ggataccgcc agttcgaaac
     2641 cttcacgacg catgttttcg ataagaacag agaggtgcag ttcaccacga ccagaaacgc
     2701 ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt accagttctt
     2761 tgttcagacg atcgaggatc tgacgagacg ttacgaactt accttcttta ccgcagaacg
     2821 gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag agtgccggca
     2881 gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg cccaggcccg
     2941 tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc agaccgaggt
     3001 ggcccagcac tttaccgact ttcgcgttac gggttttgcc ttcgctatcg atgatagtaa
     3061 cctgctggtt cggcttcact ttaccacgct tgatgcggcc aatgccgata acgccaacat
     3121 agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg tcaacgtccg
     3181 gcgcaggaac gtggtcaaca atcgcctgat acagcggggt catgtcttcc gccatatctt
     3241 cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg aagtccagct
     3301 gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca acccaatcag
     3361 gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg ccgtaagcaa
     3421 acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg tcaaccacca
     3481 gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag tcggcgtgcc
     3541 ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg tttttcgcga
     3601 ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc tcttgggttt
     3661 cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccaag gtggttttac
     3721 catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca actttgcctc
     3781 aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa caggatcaca
     3841 aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac ggagcataaa
     3901 aagggttatc caaaggtcat tgcaccaata tggtgcttaa tgtttccatt gaagcactat
     3961 attggtgcaa cattcacatc atggtgcagc ccttttgcac gatgttaagc atgataacgc
     4021 cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta cggcgacacg
     4081 gccaaaataa ttgcagattt cgttaccacg acgacaatga ccaatccagg agagttaaag
     4141 tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt ttgttgattt
     4201 gcgcttcacc gataccaaag gtaaagaaca acacgtcact atccctgctc atcaggtgaa
     4261 tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg gctggaaagg
     4321 cattaacgaa tccgacatgg tgctgatgcc agacgcatcc accgcagtga ttgacccgtt
     4381 cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca ccctgcaagg
     4441 ctatgaccgt gacccgcgct ctattgcgaa gcgcgccgaa gattacctgc gttccactgg
     4501 cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg atgacatccg
     4561 tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag gcgcatggaa
     4621 ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga aaggcggtta
     4681 cttcccggtt ccgccggtag actctgctca ggatattcgt tctgaaatgt gtctggtgat
     4741 ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg ctggtcagaa
     4801 cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc agatctacaa
     4861 atatgtcgtg cataacgtag cgcaccgctt cggtaaaacc gcgaccttta tgccaaaacc
     4921 gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta aaaacggcgt
     4981 taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt actacattgg
     5041 cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca ccaactctta
     5101 taagcgtctg gtcccgggct acgaagcacc ggtaatgctg gcttactctg cgcgtaaccg
     5161 ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta tcgaagtacg
     5221 tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga tggccggtct
     5281 tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc tgtatgacct
     5341 gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag aagcactgaa
     5401 cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcacgg atgaagcgat
     5461 cgatgcgtac atcgctctgc gtcgcgaaga agatgaccgc gtgcgtatga ctccgcatcc
     5521 ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc cggcgcacaa
     5581 cgccgggatt tagttgccgt ggaaactttc agcccatctc tgcatgggct tttttctccg
     5641 tcaattctct gatgcttcgc gctttttatc cgtaaaaagc tataatgcac taaaatggtg
     5701 caacctgttc aggagactgc tttatggcaa caggcacgca gcccgatgct gggcagatcc
     5761 tcaactcgct gattaacagt attttgttaa ttgatgacaa tctggcgatc cattacgcca
     5821 accccgccgc gcaacaactg ctcgcccaaa gctcccgcaa attgtttggt acgccgttac
     5881 cggaactgtt gagctacttc tcattaaata tcgagctgat gcaagaaagt ctggaggcgg
     5941 ggcaaggctt taccgataac gaagtgacgc tggtcatcga tggtcgctcg catatccttt
     6001 ctgtgacggc ccagcgtatg ccggacggca tgatcctgct ggagatggca ccgatggata
     6061 accagcgccg cttaagccag gaacaactac aacatgctca acaagtagct gcccgtgatt
     6121 tagtgcgcgg cctggcacat gagattaaaa atccgcttgg cggtttacgt ggcgcggcgc
     6181 agttactcag caaagcgtta cctgacccat cactactcga atataccaaa gtgattatcg
     6241 aacaggcgga ccggctgcga aatttggtcg accgtctgtt ggggccgcag ctgcccggta
     6301 cgcgcgttac cgaaagtatt cacaaagtgg ctgaacgcgt ggtaacgctg gtgtcgatgg
     6361 aactgccgga caacgtgcgg ttgattcgtg attacgatcc cagcctgccg gaactggcgc
     6421 acgacccgga tcaaattgaa caggtcttgc tgaatattgt gcgcaatgcg ctacaggcgc
     6481 tggggccgga aggcggtgaa atcattctgc gtacccgcac cgcgtttcaa ctgaccttac
     6541 acggcgagcg ctaccggctg gcggcgcgga ttgatgtgga agataacggg ccgggcattc
     6601 cgcctcattt gcaggatacg ctgttttacc cgatggtcag cggccgcgaa ggtggcaccg
     6661 ggcttggctt atccatcgcc cgtaatttga ttgatcagca ttcaggcaaa attgaattta
     6721 ccagttggcc aggtcatacc gagttctcgg tttacctgcc tatcaggaaa taaaggtgac
     6781 gtttatgcaa cgagggatag tctgggtagt cgatgacgat agttccatcc gttgggtgct
     6841 tgaacgtgcg ctcgctggag cgggtttaac ctgtacgaca tttgagaacg gcgcggaagt
     6901 actggaggcg ctggcgagca aaacgccgga tgtgctgctt tcagatatcc gtatgccggg
     6961 aatggacggg ctggcgctgc tcaagcagat taaacagcgc catccaatgc ttccggtcat
     7021 cattatgacc gcacattccg atctggatgc tgccgtcagc gcctatcaac aaggggcgtt
     7081 tgattatctg cccaaaccgt ttgatatcga cgaagcagtg gcgctggttg agcgcgctat
     7141 cagtcattac caggaacagc agcagccgcg taatattcag cttaacggcc caacgaccga
     7201 tatcatcggc gaagcgcaag ccatgcagga cgtgttccgt attatcggtc ggctttcgcg
     7261 ttcttctatt agcgtgctga ttaacggcga atccggcacc ggtaaagaac tggtcgctca
     7321 tgccctgcat cgccacagtc cgcgcaccaa agcgccgttt atcgcgctga atatggcagc
     7381 tatcccaaaa gatttgatcg aatcagaact gtttggccac gagaaaggcg cgtttactgg
     7441 cgcgaatacc attcgtcagg ggcgttttga acaggccgat ggcggcacgt tatttctcga
     7501 cgaaattgga gatatgccgc tggatgtgca gacgcgtttg ctgcgcgtgc tggcagacgg
     7561 tcagttttat cgcgttggcg gctatgcgcc ggtgaaagtg gatgtgcgga ttatcgctgc
     7621 cactcaccag aatcttgaac agcgggtgca ggaaggtaag ttccgtgagg atctgttcca
     7681 ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgc gaacgtcggg aagatattcc
     7741 ccgtctggcg cgccattttt tacaggttgc cgcgcgcgaa ctgggcgtag aagcgaagtt
     7801 gctgcatccg gaaaccgaag ctgctctgac gcgtctggcg tggccaggca acgtgcgcca
     7861 gctggaaaac acctgccgct ggctaacggt gatggccgcc gggcaggaag tgttgattca
     7921 ggatttgccc ggcgaactgt ttgaatcaac ggttgcggag agtacttcgc aaatgcaacc
     7981 ggacagttgg gcgacacttt tagcgcagtg ggcagacaga gcgctgcgtt ccggtcatca
     8041 aaatctgctt tccgaagcgc agccagagct ggagcggacg ttactgacga ccgcgttgcg
     8101 acatacgcag gggcataaac aggaagcggc gcggctactc ggctggggcc gcaacactct
     8161 gacgcgtaag ttaaaagagc tggggatgga gtgattcacg gcttgtgtgt aaagattgat
     8221 tattgagcgc aaattgctgg tattttgcgc tttactgttc cgataagttc agtatgatct
     8281 tgcccggaaa acggggagag tcattatgct ggaatcaata attaatctgg tatcgagtgg
     8341 cgcagttgac agccacacac cgcaaactgc cgttgctgcc gtgctgtgtg ccgcgatgat
     8401 tgggctgttt agctgaagct cgttgtttat gccggatgcg gagaaagtcg taggcctgat
     8461 aagacgcgac cagcgtcgca tcaggcatca tgctcagatg cctgatgcgg cgtaaacgcc
     8521 ttatccggcc tacaaaagca ataatttcaa caggttttgt tgcccagcgc atcaggcaat
     8581 cttgttctca gcacattgat ggcgctttcg ttttctactt tgtaaacgaa gcgccattca
     8641 ctctttaaat cacccgtgag aactgctgca tccgcgcttt ctggcgcaga taggtatcaa
     8701 agcacatgca aatgttgcgg atcagcaagc gacctttcgc cgtcacctgt attcccttct
     8761 catccacatc caccagccca tcttttgcta acggggcgag cagcttgaga tcttccgcaa
     8821 agtaatcagc gaagtgcaaa tcccactgtt tctcaagagg ggcgtaatcc agacggaagt
     8881 tgcagatgag cgacttaatc acatcgcggc gaatacagtc atcacgcgtt agcgcaatac
     8941 cacgccacag cgcattgcct tgttcatcca cttgctgata gtactgcttc aactctttct
     9001 ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga aacgcccatc cccagcagat
     9061 cggtatcgcc ctgagtggtg tagccctgga agttacgatg cagcacgcct tcacgctggg
     9121 ccaccgccag ctcgtcatca ggacgggcaa agtgatccat accgataaac tgatagcccg
     9181 attgcgtcag gaaggcgatg gtttcctgca ggatatcgag tttttgctgc ggactcggca
     9241 ggtcagcatc tttgattttg cgctgagcag caaaaatggt cggcagatgc gcgtagttaa
     9301 aaacgctcag acgatcgggg ttcagctccg ccacacgttt cagggtaaag gcgaaactct
     9361 ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt ggtggaggta aaaccaatct
     9421 cacgcgcatg gttaagcagt gcaaagatga actcttcatc ctgctcgcgg ttaaccagac
     9481 gttgcacttc tttgttgaag tcctgcacgc ccatgctcag gcgattaaag ccttcggcgc
     9541 gtaaatgatc gagtacatcc agttcgattt cccgcggatc gacttcgatc gaaatctccg
     9601 catcggtatt gaaatggaag ttttcgcgca gcagcttcat cagacggctg atttgcgctt
     9661 tattcagata cgtcggcgtt ccgccgcccc agtgcaattg gctgacgtga cgcccggcga
     9721 acagcggtgc acgatggacg atttcttgct ccagcgcgtc cagatactga tcggccttgt
     9781 gctgctggcg agtaacaatc ttattgcaac cgcagaagta acaaagctta tggcagaacg
     9841 ggatatgtac gtagagagat aatggacgct caggatagcg cgccacggct tgtaaaaacg
     9901 cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg gtacgaggtg tatcgtggcc
     9961 cggaatagtt atatttctgg atcagggcca gatcccagtc gatttgctgt acagacatgc
    10021 tcactccttc cgatggcgtc tctggcggct acggcgtacc ggcggatgcc cgtttcgggt
    10081 catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac cgctgtagtt taacgaataa
    10141 ccacaccaga taacatataa ccggaagggt tatcagcaga acgggaaggc ccacggcggc
    10201 aatccgttag ttgccccgca gcagacgcat catgtcttct tgcttctcgt cttcttcctc
    10261 ttcttcgtca tcgtcataag agaggccgag tttctgcatc aactcatcaa tacgatccag
    10321 tttagcatcc acccaggatt gctcttcggc gctcagggtt tcgcctgctt ccagacgttc
    10381 cagcagcgca tccagacgct catccgtttc cagtaattcc aactccgcct gcggtgaaag
    10441 cataggtttc tcactcttcg gtttgtgctg tttggtgact ttttcagtca ctcccaatgg
    10501 aataggggtt ttactgccaa tacgtggatc ttttggtgcg ttctggcctt tgctgcctga
    10561 cgtggtgttt ccgcccgctg cgcggctgcc cggcgcatga ccacgacgtt ttttctgacg
    10621 cttacggtca cgagcttcct gatccagctc ctcgcgtgtt tttcgacgcg cttttgcatg
    10681 acctttgctg cgtgagtttg aagatgatgg tttcataatg gttcgtcttt tagccgtttt
    10741 attcagtata gatttgcggc ggaatctaac agaaagcaag caaagaaaaa aggcgacaga
    10801 ttactctgtc gccttttttc ctgactcata acccttaacg ggtcttacaa tccttgcagg
    10861 ccgccaacac accctgtttt tccattagcc ggaacaatcc gtgttgattc catttccgtt
    10921 taattacgtc tccggacgtt tgtcttcctg acaatcctct gtcttcgcct cctggcgctc
    10981 ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc gctctatggg tgctacttta
    11041 cgcctttgct ttaaacaaac aatcaaccgc catcagtatt tgcacatttt cagagtttta
    11101 tataaaagca attcactgat ttatatagct tatttgacat gcatcgcaga attttctctg
    11161 ctatttcgca taaagactat cggcaatgtc tcacaaagcg tatggctttt acttacaacg
    11221 ctcacgccga aaaatccgcc ttttattatg gttcaggtat aatcgccaca aagctttgac
    11281 tgacggagac gaccgctttg actaatttga attatcaaca gacgcatttt gtgatgagtg
    11341 cgcctgatat tcgccaccta ccttccgata ccggaattga agtggctttt gcaggccgtt
    11401 ccaacgcagg taaatccagc gcgctgaaca cgctgactaa ccagaaaagc ctggctcgta
    11461 cctcaaaaac cccagggcgc acccagctta tcaacctgtt tgaagtggct gacggcaagc
    11521 gtctggttga cttgcctggg tacggttatg cggaagtccc ggaagagatg aagcgcaaat
    11581 ggcagcgtgc gctcggcgaa tacctcgaaa aacgtcagag cctgcaaggt ctggtggtgc
    11641 taatggatat tcgccatccg ctgaaagatt tggatcagca gatgattgag tgggcggtag
    11701 acagcaatat cgccgttctg gtgctgctga ccaaagcgga caaactggca agcggcgcac
    11761 gtaaagcgct attgaatatg gtgcgtgaag ctgtactggc gtttaacggt gatgtgcagg
    11821 ttgaaacgtt ttcttcgttg aagaaacaag gcgtagacaa gctgcggcag aaactggata
    11881 cctggtttag cgagatgcag cctgtagaag aaacgcagga cggcgaataa ttttcttgcc
    11941 ttaatgcttg tgccggatgt ggcgtatccg gcccgtaaat tcaatagatt acaattctac
    12001 cgttggcctg ataaacatag cgcatcaggc attacggatc ttttctttcg cccaataaaa
    12061 aacgccccag tcattactga ctggggcggc taaaatattc agccaaatcc gattacgtga
    12121 agtaaaaggt ctgaaagata gaacatctta cctctgtacc ctacgcgact aactttactc
    12181 ttttttgttc agttcagaaa gcactttttg taattttttt tcagttgttg cataggaatt
    12241 tctaatagcc atcacaaaac gtgacagctt atgttgctta cttacgaaaa aagggcatgt
    12301 tcaggcgaat cttagtgcgc ctgatcccag ttttcgccac tccccacttc caccagcaac
    12361 ggcacatcca gacgggtaca gttttccatc agttgatgaa tctgcttcgc gacggcatca
    12421 acatcatctt tatgaacttc aaataccagt tcatcgtgta cctgcatgat catacgtaca
    12481 cgcggttgct cagcctgtaa ccacgcatca acggcaatca tcgcccgttt gataatgtcg
    12541 gcggcggttc cctgcattgg cgcgttaatg gctgcacgtt cagccgctgc acgacgagca
    12601 ccattgctgg atttgatatc cggcagatac agacggcgtc cgtccagcgt ttcaacgtag
    12661 cccagctctt tcgcctgagc acgggtgcgt tccatatact ccagcacgcc agggtagcgt
    12721 tcgaagtaaa ggtccatgta cttctgcgct tctttacgtg gaatgttcaa ttgccgcgcc
    12781 agaccgaaag cactcatgcc ataaatcaga ccaaagttga tcgctttcgc gctacggcgt
    12841 tgctcgctgg tgacggtttc cagtggcaaa ccaaacactt ctgccgccgt tgcccggtgg
    12901 atatcttttc cttccgcgaa tgcggtcagc aagcctttat cacgcgagag atgcgccata
    12961 atgcgcagtt caatctgcga gtagtccgct gagacaatca cataatcctc tggcgcaata
    13021 aacgcctggc ggatacgacg accttcttcg ttacgcaccg gaatgttttg caggttagga
    13081 tcggttgacg ataaacgtcc cgttgcagtt actgcctggt gataagaggt atgcacacgc
    13141 ccggttttcg ggttgatcat cagcggcagc ttgtcggtgt aagtcgactt cagcttcgcc
    13201 agaccacgat actccagaat cacttttggc aacggatagt ccagcgccag ttcttccagt
    13261 acctcttccg acgttgacgg cgcgccaccc ggcgttttct tcagcggttt aataccctgt
    13321 ttttcaaaca gaatggtttg caactgcttg gtggaagaaa ggttaaattc ttcacctgca
    13381 atttcatgcg ctttcttttc cagctcagcc agacgcaagg tgagctcttc agaatgattg
    13441 tgcagcactt tcggatcgat cttcacaccg ttacgttcaa tgcgtgaaag caccggcacc
    13501 agcggcattt cgatattctc gaagacgttc aacggccctt tgtgtttttg cagatccggc
    13561 cacattttca gatgcaactg caaggtgaca tctgcatctt cggcggcgta acgtccggcc
    13621 tcttcgaggg caatctggtt aaaggtcagt tgatttttgc ctttacccgc aatctcttca
    13681 aaagtgatgg ttttgtgctt caaccaacgt tccgcgaggc tgtccatatc gtgacgcccg
    13741 gcaacgctat tgagaatgta ggactccagc atggtatcaa acgcaatccc acgcagttca
    13801 atgccgtaat tcgccagaat accgcgatcg tatttcaggt tttgcccgac cttcagcgcc
    13861 ttttcatctt ccagcagcgg ttttagcaac tcgagtgcac gctcgtgaga gatttgatcg
    13921 ggcgcatcaa gataatcatg agcaaccgga atatatgccg ctacgcctgg ctcgatagca
    13981 aaagaaagcc cgaccaggtt agcagagatg ttatcaaggc tgtcggtttc ggtatcaaat
    14041 gcaaataccg gcgctttttc cagcttcgca atccacgctt tcagtgtttc ttcatcaagg
    14101 atagtgacgt agttgtcata agaaatcacc gttgccgtca cttctggcgc ttcgtctgca
    14161 acactggttt cctgcggctt cgcggctggt tttgcccctt tggcctgtaa ccatttgccc
    14221 gcttcgacat cagcagtcca gcgtttgaac tcatactttt tgaacagccc caacaactct
    14281 tccgctgccg gttgctgcac ttccagttgt tcacaggtca gctccagttc aacatcggtt
    14341 ttaatcgtcg ccaactggta tgagagataa gcaacttctt tgttttgctc gagcttcgct
    14401 gccattgttt tcgcgccacg gaagctcaac ccagcaattt tttctggctc ggtatacagc
    14461 gtatccagcc cgccaagacc ttgcagcaat gcctgcgcgg ttttttcacc gacgcccggt
    14521 acgccaggaa tgttatcaga ggagtcaccc atcagcgcca ggaagtcgat gatcaattct
    14581 ggcggcacgc cgtacttatt caccacctct tccggtccga ggatggtatt cgtcatggta
    14641 ttgataagcg taatatttgg cgtcaccagc tgcgccatat ctttatcgcc agtgctaatc
    14701 agcaccggac gcccggcttt ttcggcttcg cgcgccagag taccgataac gtcgtccgct
    14761 tctacgccag aaaccgccag cagcggcagt cccatcgctt taaccatcgc gtgcagaggt
    14821 tcgatttgtg ctcgcagatc gtccggcatt ggcgggcgat gtgatttgta atgttcaaac
    14881 agttcatcac gaaaggtttt tcccttggcg tcaaagacca ccgctgcatg cgtcggttta
    14941 tattgcatga tcagactgcg cagcatgttg aggacaccat acatcgcacc ggtcggctcg
    15001 cctgcgctgt tagtcagcgg gggaaacgcg tgatatgcgc gataaagata agatgaacca
    15061 tctacaagga taagtggatt ttgggggatc tgaaccataa tgtccgtgcc tgtttatcag
    15121 attatgggta aaggatgcca cagacaggat gaaaacatga gtttttcacc gcttttcgtg
    15181 aaaatctttt gtagatctgc tggatcgctc gcaaatcgtc atgtggataa ctttgtgcat
    15241 aaattattta catcgctttt aatttattat catgcttttt acagcatgat gtttgtcttt
    15301 atttttcatg cagttaaaaa taaatccatc cctaataatt gagataatgg gattttttct
    15361 aatgtggata gatatgaatt atttttctcc ttaaggatca tccgttattt gggtcatttt
    15421 ctgctaaaat ctgcgccttg cgcttcttta gtgcactgtc tatggctaac cttttgaata
    15481 aattcattat gacgagaata ctcgctgcga taaccctttt gctgagtatc gtattgacca
    15541 ttttggtcac tattttttgt tccgtcccga tcatcattgc cgggattgta aaactattgc
    15601 tacctgtgcc agtcatttgg cgaaaggttt cgcgtttttg tgattttatg atgtattgct
    15661 ggtgtgaagg tctggcggta ttactgcatc tgaacccaca cctgcaatgg gaagttcacg
    15721 ggctggaagg gttaagtaag aagaactggt atctgcttat ttgtaaccac cgtagttggg
    15781 cagatattgt cgtactgtgc gtgctgtttc gtaagcacat tccgatgaat aaatatttcc
    15841 tcaagcaaca gctggcctgg gtgccatttc ttggcctggc gtgctgggcg ctggatatgc
    15901 cgtttatgaa gcgttattcc cgcgcttatt tattacgtca tccggagcga cgtggtcaag
    15961 atgttgaaac cactcggcgt tcttgtgaaa agtttcgcct gcatcccacc accattgtca
    16021 atttcgttga aggctcccgt ttcacgcaag aaaaacatca gcaaacccac tccacttttc
    16081 aaaacttgtt gccaccaaag gcggcaggca ttgcgatggc gctaaatgta ctgggtaaac
    16141 aattcgataa actgcttaat gttacgctgt gttatccgga caataaccgt cagccgttct
    16201 tcgatatgtt aagcggtaaa ctgacgcgga ttgtcgtaca tgtggattta cagcctattg
    16261 ccgatgagtt acatggcgac tacatcaatg acaaaagctt taagcgccat tttcagcaat
    16321 ggctgaactc attgtggcag gaaaaagacc ggcttctgac gtctctcata tcatcacagc
    16381 gtcagaataa gtaagtttta ctcgcctctg aatgagcaga ggcgagtgag tattttaatg
    16441 aacaaaacgt ccggcacgag acataaattc ttcttcgctc atctcctgtc cgttaaaaac
    16501 aactttaccc ggtgtataac gcagctgcag cgaggcggta ttgttgtcaa tcgtgattaa
    16561 ctgaaacatc tgacctaatg tcatcatccc gctgatttgt ttatcagcct gcttttgcgc
    16621 tttttccgca tccattcctt cagataaatt aagctgtttt gcggtttatg tgacgacatt
    16681 aagcggtaac ttcacatcaa aattgagcga tttgatatct ttgtttgtgg atgatgaaga
    16741 tttggctggg tcggcaatac tgatatccag attggcattt agttcgccga gtgcgttctt
    16801 ccatcttacc ggttgtttaa tggtcggctc acttttttgt aataacggta aatactcttt
    16861 gaacaatgca gcattcactt cttgcagagc aacttcatcg tttgctaact cagggtgtgc
    16921 agcaagctgc ttctgcatcg caatgttata ctggatgata aattggcgca ctgctgaggg
    16981 atcaatagat tcagcaatca atgaccactc accactaccg agttgctgat tcccttttgt
    17041 taacttatca agttcgtagg tcagattgac attgatataa tctttgtctt taacgcgatc
    17101 cagcctggtt tttgcaacga aagcatcgat ttgggcaacg tcttccccgt gattgataat
    17161 gttaatctga ttaaatttcg actcgctttc accaaccgga aatttctttt cttctaatcg
    17221 cgccagagaa tcaatggtaa aactttttgc cgttactttg ttcattggtg ataattgcag
    17281 aacaatatct tcgactttgc cttcaatatc caggttagac aatgaactac catcaccatt
    17341 aaaagtaaac tggccctcac caaaagaaaa ctttccatac tctgcagggg caacatctaa
    17401 tgtcgtagag aatgagccac caaacgcgaa tcgcaatgtc gcttccacgg gcgatttatt
    17461 tttcgcggcg ataaatagcg gttgtgtcag ttcattattc actacgttca cttttgccgc
    17521 tgccaatgct gggatgacat taccatgcat taacattgtg atgggcaacg gaccgtgctc
    17581 aacatccaca tcaaatacaa ctttctggcc tggcttgata ttgagatcgg gtgcaccgtt
    17641 atcgaatgtc atttgcattt gaatacggga attaaaaaag cctttgtcaa agtttttata
    17701 acttaacgtc atatcgtagg catgttcacc tttctttttc gcatcgttaa aatctttaat
    17761 aaacttttcg ataccaggct gaatttgcgt acctgtgtac catgtgccac caccccagat
    17821 tacggctaac gcaacaataa cacctgtagc tgacttacgt atcatataaa tattccctga
    17881 taaaacagca aaactttgaa tatccactta tgctgaaata atgtccagcg gcaggatgca
    17941 ttatcagaat gtatttatat ttattaaata ataaaaaaag cccgtgaata ttcacgggct
    18001 ttatgtaatt tacattgaat tattttttct cggacagata tttcaccgta tcagcatact
    18061 gctgaacaaa aacatccata ttgctggtat ccataccctg cggattcagc tgatatttac
    18121 cgttaacaaa catcgccgga acaccacgca attgcacatc agctgcggct ttttcctgct
    18181 gagcgaccag agatttcacc acgaagctgt tccacgccgc gtcgtactct tcacctttaa
    18241 tacctgcatt gataaataca tcgcggatat cagatgctga acgaatggtc tgggttttct
    18301 gtacgccttc aaacagcgga acagtcactt tgtcttccac gcccagcgcc atcgccacag
    18361 cccatgcctg agtcagatct ttgcccaggt caccacccat gaagttgacg tggtatttag
    18421 tcatcttcac gccttccggc agttttttct tcacattatc agaaatatgc agaacttctt
    18481 caaactgata gcagtgcggg cagaagaaag agaaaaactc cagcacttgc ggcgcgccag
    18541 ctaccggttt ttccagggta gtgtactgtt taccatcttc atactgcgcc gccgatgcgc
    18601 taaacgctaa aactaaacca gccagcgcca gccaaatctt tttcatgatc tactctctcc
    18661 gattaataca taggtgttaa ttgcaaaggg ggttcttgta gaacttttgc ctgttctata
    18721 aaagtcgccg tctgtcgtag ccagtaatct tccccggtta accacgggaa atttttcggg
    18781 aacgcgggat cagcccaatg ccgcattagc caggcaagat aataaaccaa acgcatggcg
    18841 cgtaaaggtt caatcagtcc gatttcagcg gtgtcgaact cgctaaattc ttcataagct
    18901 tcaataatag tttccagttg catccgctgc tgggctttat cgccattgag caacatccac
    18961 aaatcctgaa tagctggacc attacgtgca tcatccagat caacaaacat tggaccatcg
    19021 cgccagagaa tattcccggc gtggcagtct ccatgtagcc gcagaacggt gaaatcttcc
    19081 cgccagtgtg ctgtaacggc ggcgatcagc tcatctgtcg ctttcagaaa tgccgctttc
    19141 aacccggaag gtatcagtgt agcgtcctca aacagcttgc gaggctcaat gagatattca
    19201 ttcagaccga tggtctggcg atgaataaaa agctgtttgc gccccgtctg gtgcatacgc
    19261 cctaaataac gcccaaccgc ttccatctga tcgatattat cagcttcgaa ctggcgacca
    19321 ccgacgcttg gaaaaacagc gaaataaaat ccctgatgat tcaataaagt ctgaccgtta
    19381 aaggccacag gtgctgcgac cggaacttca tcatttacca gctgcaacgc aaattgatgt
    19441 tcttcgagaa tttgatcggc tgtccaacgt tcagggcgat aaaatttgac gacaaaacgt
    19501 cgacgctctt cgtcctgaaa ttgatagaca cggttttcat agctgttaag cggggtaaga
    19561 ccggaatcca cccggatccc ttgctcaaac agagcgtcca tgatggtatc cgggtgtagt
    19621 gtctggaaag taaaagcgct gttgttcatc ccatcatccg gaaaacatta cgaatgatga
    19681 aggatatcat ttcacgatga ttttggtggc gccgcttaca agcttttact ctttaattac
    19741 gccgcgagcg cgtaacagcg ccgttttgaa gtcttcttca taatctttct gcaaacccgg
    19801 gatcaccgca tctttcgcgg aatcacgcat tttcaggtga tagatcagaa tgtcatccgt
    19861 caaatccgcc agttcgccgt caaaacctga ctctttcgcc agtttctgca aaaattgcaa
    19921 caagttaaag tctggctctt tttgccaggc tggctggagg agttcaataa cttcattcag
    19981 acgtttacat ttcatagtaa tgctccttac tcttgagaca gacacgttag cagggtcaat
    20041 cccacaataa aagaggcgat atcggtgaat ctgatgacga cgataacagg cgttgtgctg
    20101 gcaggcggta aagccagacg aatgggcggc gtagataaag gattgctgga cttaaatggc
    20161 aaaccgctgt ggcaacatgt cgctgacgcg cttaagacgc agctctctca cgtcgtggtt
    20221 aatgctaatc gtcatcagga aatctatcag gcaggcggtc tgaaagtgat tgaagattca
    20281 ctggcggatt acccaggccc tctggcagga atgctttcag taatgcagca ggaagctggt
    20341 gagtggtttt tgttttgtcc gtgcgatacg ccttacattc cccatgattt agccgcccgg
    20401 cttaatcatc agcgcaaaga tgcgcctgtc gtgtgggtcc atgacggtga acgcgatcac
    20461 ccgactattg ctctggtaaa ccgcgctatt gagcctttat tactggaata tctgcaagca
    20521 ggagaacgcc gggtaatggc atttatgcgt ctggctggcg gtcatgcggt tgatttcagc
    20581 gatcataaag atgcatttgt taacgtgaat acgccagagg agcttgcccg atggcaggaa
    20641 aaacgatgat accgttactc gcctttgccg cgtggagtgg taccggaaaa actacgcttt
    20701 tgaaaaaact gatcccggca ttatgcgcca gagggatccg tccggggctg attaagcata
    20761 cgcaccatga tatggatgtt gataagccag gcaaagatag ctatgagctg cgcaaggctg
    20821 gcgcggcaca aaccatcgtt gccagccagc aacgatgggc cttgatgacg gaaacaccag
    20881 acgaagaaga gctggatcta cattttctcg caagtcgaat ggatacctca aagctggatt
    20941 tgattctggt cgaagggttt aagcatgaag agatcgcaaa gattgtgctg tttcgcgatg
    21001 cagccggaca tcgacctgaa gaattagtga tagacaggca tgttattgct gtagccagtg
    21061 atgtgccgct taatcttgat gtcgcattac tggatattaa tgatgttgag gggctggctg
    21121 attttgtggt ggagtggatg caaaagcaga acggataagc aagagtaggc tggatcagac
    21181 gcagccagtg tcgcatccga cctttttctg caccagcgca aaaaggcccg actgggcct
//
LOCUS       DAJVQH010000065        21113 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJVQH010000065 DAJVQH010000000
VERSION     DAJVQH010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.065"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            5..838
                     /locus_tag="OQM87_002423"
     CDS             5..838
                     /locus_tag="OQM87_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508527.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEADRAK
                     QVALIAAAQDAETKAVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALN
                     DAINVLSDEQTSLKFKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDAN
                     TGNVGGGNLAEQALSAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENV
                     E"
     gene            1077..1136
                     /locus_tag="OQM87_002424"
     CDS             1077..1136
                     /locus_tag="OQM87_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1508528.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1453..1512
                     /gene="ibsE"
                     /locus_tag="OQM87_002425"
     CDS             1453..1512
                     /gene="ibsE"
                     /locus_tag="OQM87_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCS1508529.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1632..3065)
                     /gene="hldE"
                     /locus_tag="OQM87_002426"
     CDS             complement(1632..3065)
                     /gene="hldE"
                     /locus_tag="OQM87_002426"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCS1508530.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3113..5953)
                     /gene="glnE"
                     /locus_tag="OQM87_002427"
     CDS             complement(3113..5953)
                     /gene="glnE"
                     /locus_tag="OQM87_002427"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCS1508531.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(5976..7277)
                     /gene="ygiF"
                     /locus_tag="OQM87_002428"
     CDS             complement(5976..7277)
                     /gene="ygiF"
                     /locus_tag="OQM87_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCS1508532.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIVSAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7519..8139
                     /locus_tag="OQM87_002429"
     CDS             7519..8139
                     /locus_tag="OQM87_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCS1508533.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            8203..9441
                     /gene="cca"
                     /locus_tag="OQM87_002430"
     CDS             8203..9441
                     /gene="cca"
                     /locus_tag="OQM87_002430"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCS1508534.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9604..10425)
                     /gene="bacA"
                     /locus_tag="OQM87_002431"
     CDS             complement(9604..10425)
                     /gene="bacA"
                     /locus_tag="OQM87_002431"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1508535.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10516..10884)
                     /gene="folB"
                     /locus_tag="OQM87_002432"
     CDS             complement(10516..10884)
                     /gene="folB"
                     /locus_tag="OQM87_002432"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCS1508536.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            10989..11606
                     /gene="plsY"
                     /locus_tag="OQM87_002433"
     CDS             10989..11606
                     /gene="plsY"
                     /locus_tag="OQM87_002433"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCS1508537.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11619..12550)
                     /gene="ttdR"
                     /locus_tag="OQM87_002434"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(11619..12550)
                     /gene="ttdR"
                     /locus_tag="OQM87_002434"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            12757..13600
                     /gene="ttdA"
                     /locus_tag="OQM87_002435"
                     /pseudo
     CDS             12757..13600
                     /gene="ttdA"
                     /locus_tag="OQM87_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13597..14202
                     /gene="ttdB"
                     /locus_tag="OQM87_002436"
     CDS             13597..14202
                     /gene="ttdB"
                     /locus_tag="OQM87_002436"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCS1508538.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14250..15647
                     /gene="ttdT"
                     /locus_tag="OQM87_002437"
     CDS             14250..15647
                     /gene="ttdT"
                     /locus_tag="OQM87_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCS1508539.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALMLLLRIISWDDIVSNKAAWNVFFWLASLITLATGLNN
                     TGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRYFFASATAYTSALAPMMIAAALA
                     MPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLIFLV
                     LLVITGLLWMPVVLL"
     gene            complement(15690..16703)
                     /gene="tsaD"
                     /locus_tag="OQM87_002438"
     CDS             complement(15690..16703)
                     /gene="tsaD"
                     /locus_tag="OQM87_002438"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCS1508540.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            16941..17156
                     /gene="rpsU"
                     /locus_tag="OQM87_002439"
     CDS             16941..17156
                     /gene="rpsU"
                     /locus_tag="OQM87_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCS1508541.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17267..19012
                     /gene="dnaG"
                     /locus_tag="OQM87_002440"
     CDS             17267..19012
                     /gene="dnaG"
                     /locus_tag="OQM87_002440"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1508542.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19207..21048
                     /gene="rpoD"
                     /locus_tag="OQM87_002441"
     CDS             19207..21048
                     /gene="rpoD"
                     /locus_tag="OQM87_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCS1508543.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5083 a         5844 c         5419 g         4767 t
ORIGIN      
        1 gtttatgacg cttgagcagg agcagcaggt taaaacccgt actgccgaac agaatgcacg
       61 tattgcggct tttgaagctg aacgtcgtcg tgaagcagag cagacgcgaa ttctggctga
      121 acgacaaatt caggaaacgg agatcgagcg cgagcaggcc gtccgctcaa gaaaggttga
      181 ggctgaacgt gaagttcgta ttaaagagat cgaacagcag caggtcaccg aaatcgccaa
      241 ccagacgaaa tcgatcgcta ttgccgccaa atcggaacaa cagtcccagg cagaagccga
      301 tcgtgctaaa caagttgccc taatcgctgc cgcgcaggat gcagaaacca aagcggttga
      361 actgaccgtg cgggcgaaag cagaaaaaga agccgcagag atgcaggcgg cggctatcgt
      421 tgagttagcc gaagctacac gtaaaaaggg tctggcggaa gcagaagcac aacgtgcgct
      481 gaacgatgct atcaacgtac tttctgatga acaaaccagc cttaaattca aactggcctt
      541 gttgcaggcg ctacctgcgg taatagaaaa atccgttgag ccgatgaaat ctattgacgg
      601 tatcaagatt attcaggtcg atggtctgaa tcgtggcagc gctgcgggtg atgcaaacac
      661 gggtaatgtg gggggcggaa acctagcgga acaagcatta tcagccgctc tctcttaccg
      721 cacacaggct ccgctgattg actccttgct caatgaaatt ggcgtttcag gcggttcact
      781 ggcggcattg acttcaccct taacctcaac aactcccgtc gccgaaaacg tagaataaat
      841 tttcagctat gaacgtccct ggcacaaaaa gccggggatt ttttatatct gcgttccgct
      901 aaaaactgca aatgatcagg tcgttgcaac gttttgcgtg accgctcggg gaaggcaaaa
      961 ctgcctctgg gaaagcgttg cgcgaggtcc ggcgctcatc aacaatcggg gagtcagcaa
     1021 agggctgaaa cgggaaagcc cctcccgagg aaggggcctt acagaaggaa agggttatga
     1081 tgaagctcgt catcatactg attgtgttgt tactcgtaag tttcgcagct tattaacagc
     1141 caatcagagg ggggagaaat cctccctcac ccttgttcct ttactttagg ttgaaaaaaa
     1201 atcagcgtca agaaggtttc attaacacat atccctggcc caaaaagccg gggatttttt
     1261 atatctgcgt tccgctaaaa ggtgcaaatg ctcaggacgt tgcagcgttt tgcgtgaccg
     1321 ctcggggaag gcaaaactgc ttcagggaaa gcgttgcgcg gggtccggcg ctcatcaaca
     1381 atcggggagt cagcaagggg ctgaaacggg aaagcccctc ccgaggaagg ggccttatag
     1441 aaggaaaggg ttatgatgaa gttcgtcatc atactggttg tgttgttact gttaagtttc
     1501 ccgacttact aacaactgat cagagggggg agaaatcctc ccttaccctt gttcctttac
     1561 tttaggttga aaaaacaaca gcgtcaatag gcctgcctgc tacgaagcga gatctgtgaa
     1621 ccgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt agtggtcgag
     1681 caaccgtctt cgaagttgag caccagcact tcgccgccgt tggcccagac ttctttactc
     1741 ccggcgatct cttctggttt atagtcgccg cccttcacca gtaaatctgg caggatcccg
     1801 gcgatcaagc gctgcggcgt gtcttcttca aacgacacca cccagtcgac cgcttccagt
     1861 gcgcccagca caatcatacg ctgttcgagc gggttaacgg ggcgggaatc ccctttcagc
     1921 cgtttggtgg aggcatcgct gttcacagcg acaatcaagc ggtcacccag tttgcgggca
     1981 tttgccagat agaagacgtg tccggcgtgc aggatgtcaa agacgccatt ggtcatcacc
     2041 actttttcac cgcgtttacg cgctgccgct acggccagct tcagttcctc ttcggtcatc
     2101 acgccaaagc cggtatctgc gcgcccacgc actgcatttt ccagctcgat cggcgaaacc
     2161 gtggaggttc ccagtttgcc gaccaccacg ccagccgccg cattggcaaa gaagcaggct
     2221 tcttccagcg aattacccgc tgccagcgtt gccgccagga cgccaatcac cgtgtcgccc
     2281 gcaccggtaa cgtcatacac ttcctgcgcc tgggttggca tatgcagcgg cgctttaccc
     2341 ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga gagttcgtaa
     2401 tcggcaatca gtttcatgcc gcgctcaaca atctcttctt cggtcttaca tttaccgaca
     2461 acagcttcaa attccgagag attcggtgtc agcagcgtag cgccgcggta gcgctcaaaa
     2521 tcggtacctt ttggatcaat cagcaccgga acgcccgctt tacgcgccag ttggatcatc
     2581 tgctgtacgc ttgccagcgc acctttggcg tagtcagaaa gcaccagcgc gccaatcgaa
     2641 ctcagcgcct gattaatccg ctcgtgcagc ggctgcggat caacaccttc gaaaccttct
     2701 tcaaaatcca gacggatcag ctgttggttg cgggaaagta cccgtaattt ggtgatggtc
     2761 ggatgcgtcg gtacagaaac gaagtcgcat ttgacgttga cgtcggccag agatttactc
     2821 agcgcgcgcg ctgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt agcaccgaga
     2881 gaagcgatat tcatcgccac gttagccgcg ccgcccggac gttcttcgat ggtattcact
     2941 ttaaccacgg gcaccggcgc ttccggcgag atacgactgg tggggccgta ccagtaacga
     3001 tccagcatca catcaccaac caccatcact cctgcacgtt caaactctgg cagcgttact
     3061 ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata cttcattctt
     3121 ccaccagcca cttctgccag cttgcccgca ccagatcacg ctctgcggtg aagcaatcct
     3181 ccgacacatg gcccggcaat tcctgtaatg ccagatgatg aagttcatcg cgcagcgtcg
     3241 tgtaagcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt tgcgccagca
     3301 gctccagaat acgcacgttg tctgaccagc gcgttaactt cggtttttca tgagcgtagc
     3361 gcaacaccag atattgggta ataaattcga tatcggtaat tccgccttca tcggctttga
     3421 tatcaaagcg attacgatgt ttattgccga gatgagcgcg cattttctcg cgcatttccc
     3481 gcacttccgt ttgcagagtt ttaccttctc gcggcagtgt cataatctca cgacgcaccg
     3541 cgtcaaagtg cgcagtgagc tgcggatcgc cgtacactac acgcgcacgc accagcgcct
     3601 gatgttccca cgtccaggcc tcgtttttct gataatcggc aaatgctgct gcggatgtca
     3661 ccagcattcc cgccgccccg gacggacgca gtcgagcatc cacttcatac aaaatgccgg
     3721 aagaggtacg cgtactgaac agatgcataa tgcgttgcgc cagacgcaga taaaactgcc
     3781 gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat tgggcaatca tggaggaaga
     3841 taaggtcaag atcggaactg tagcctaact cccagccgcc cagcttgccg tagccgacca
     3901 ccgcaaaacc gcgcccttca cgttcgttga ggtgatttgg cttaccgtag cgggccacca
     3961 tttgacccca cgcctgctga acgacggcat ctatcatagc ttctgccagc caggttaagt
     4021 gatcgctcac tttcattacc ggtagcgtac cggcgatatc cgctgccgca atgcgtaaca
     4081 gctgcgcctg tttgaactga cgcagcgcct caagctgttg ttcttcgtca tcttccggca
     4141 cgcgcagcaa atactggcgc agttcatcac ggtaggcatc ggtcgccgtc ggctggtaaa
     4201 gggtatttgg atcgagcaat tcatccagca ataatggata acgcgctagc tggctggcaa
     4261 tcatcggcga tgcggcacac agagaaatca aatgtttaag cgccgcaggg aattcactga
     4321 gcaattctaa ataggttgtg cgggtaacaa tccccaccag caaggcggta atgcgcgaca
     4381 gcgtgacagc agcgtcttca cgcgcacaga catcacttag cagatgtggc atcagatggt
     4441 cgagcacctg acgtcctcgc ggcccgatag tacgcttatc cagctctttg cggaaatcag
     4501 caatcagcgt taggacctgt ttgcgatcat cctcgctaag atgcgccagc actggcgtag
     4561 tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac agcgactctt
     4621 cctgagtttc actttcgtcg tcgccaatca attcattaaa cacccggcgc acattggtca
     4681 tatgtgcggt cagcgccccg gtcagttgcg gccagtcagc aaagtccatc gcccacgcca
     4741 gccgcgcacg gttaagctca tcagaaggaa gcgtttgggt ttgttcgtcg ttaatgcttt
     4801 gcagcaggtt ttccagacgc cgcaggaaca gatatgccac tcgcaattgt tcagcatcgt
     4861 tttcagaaag cagatgcagc gcggcaatgg cgctgagcgt tggcagtaaa gagcgcgact
     4921 gcagcaacgg ttcgcgccca ccgcgaatga gctggaacac ctgaacgata aattcaattt
     4981 cgcgaatgcc gcctgcgccg agtttgatat tgtcggtcaa accacgtcga cgcacttcac
     5041 gggcaatcat ccctttcatg ttgcgcagcg actgaatcac gctgaaatcg atgtaacgac
     5101 ggaaaacaaa cgggcgcagc atcgcacgca actcgttagc atagacgcct tcgctatcgc
     5161 ccataatccg cgctttgacc atcgcgtaac gctcccagtc gcgtccctgc tcctggtaat
     5221 aatcctccaa cgcggcaaag ctcagcacca gcgggccact ttcgccaaac ggacgcagtc
     5281 gcatatccac gcgatagacg aagccatcct gcgttggttg atccagcact ttaatcagcc
     5341 gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg ccctgcgtac
     5401 aaccatgttc cggccaggca aaaatcagat cgatatcaga ggagaaattc agttccccgc
     5461 cgcccagctt acccatgcct aagatcagca gcggttgcgc ttcgccctgc gcattgcacg
     5521 gcgttcccca ctcgcggcag caggcgtcat acagccagtc acgcgccgca acaatcagcg
     5581 tctccgccag ataactgagc tgctgcaata tgctctcttc agtaaccagt gccagcgttt
     5641 gcgcccatgc gatgcgcacc ataatgcgcc gccggaatag ccgcagctcg cgcattaacc
     5701 cggcttcgtc gctcacatta ctgagcgcct cctgcaacca cacgctgtaa tgttgccatt
     5761 cgtcagcctg tggcggttgg ctttccagtt ccgtcagcca ctctggatgc gcaatcacgc
     5821 tgtcctgcac aaaatcactg aaagtaagta ctgacttcgc ctgtgcacta agtgattcct
     5881 cggctaaaag ctctggcagc cgctcaacaa cggtctgcca gtactgctgt aacggtgaag
     5941 agagcggctt cataagcgat tttatccttg cctgattaac gttttccgct gtgcaaccag
     6001 aacggttcct gattgtttgc ctcattacgg aaatgttcaa tttcaatgcg ttgcccggtc
     6061 gcaatagcgt gacgcagccc ctgccagttc tccagccagg cttgcgcgac gacaggatca
     6121 tagtaacccg ccagcaacag tattgagtca atatcacgca tcaggcgtgg caactggtca
     6181 cggtagcgat cgcctaacgg ctggcagaaa acgcttttca gttcagcggc atggcgggaa
     6241 agatggatat cggcaaagcg tttgaaggag tcgctaattt tgccctgcgc tttggcatct
     6301 aaaaacggct gccatgcttt gcttaccagc cattcggtca acgccagctt cgccattgcc
     6361 gtttcggtag agtagaccgc cgtcacggca gaaaccgcag aaacgatggt cgcttcgcat
     6421 tgagtcagca aatcacgtaa gtgagtgctc gctttacgcg gcacaatgcc accgaacagc
     6481 atcagtgtat gacggactag gctaatggct gccagcacct gctctttcgc cgcatcgttg
     6541 ccgcgtaccc acagttcttc atgatactgc cattgcgcta acaccagctc gagcgccgct
     6601 tccagcccct gttccacatc ggcttttgcc gcaacatgca aaatggtggt cggtttgatt
     6661 tcacgcgccg gattgccctg cgccagatga tagccacgcg ccgctttgct caggctgccc
     6721 tggcgtaatc cggtttgcga taccagttgg ttcgccagtt tcagcaccgc gcgcgtgtcg
     6781 ccgctaagca gctccagttc cagctcgcag ataggctcag caaattcacc cgctttcact
     6841 tccccctggt cgagagcgat ttcaattcgg ctgccatcga tctctactag ccatttttcg
     6901 cgataaaaat cggtgctaaa cagcggctgc acgcgggaag cgagatcggc aggcaattcg
     6961 ccgttcggcc agatttccgt cggtaactgc gccaggtcga gcgtcggttc gctcaacgcc
     7021 acgttatatt ccgggcgctg gtgtaagccg cctgtcactc ttcctgcaac tttcatggtc
     7081 atctcatagc gaccgttttc gccacgaata cgtaagccca tatcgtgcct acgcagccag
     7141 ttatccggcg tttcgtagta aatattcagc aactgcacgg ggtcatggtg ctcgccgccc
     7201 agcgtattga gatggtcacg caacgcctca acggcactgt gattaacaat aaactttaat
     7261 tcgatttcct gagccatggc ctttacttat cggttatgtc acataagtga cgatgaacgg
     7321 cgaacttaat gcgatctttt ttgtcagtag atagtatttt gcgccaaatt gccatgcaac
     7381 gggcaatttg acgggcgtaa aagtttgaag cagtggcaag acctgacaca gaggattccg
     7441 attgatgtcg aatgctttgc gtagcgacac tgatgccact actatcgttc cactttccat
     7501 gacaataacg acagcctgat gccaaaatta cgcctgatcg gattaacttt actcgcactt
     7561 agcgcgactg ccgtctcaca cgctgaagaa acgcgctatg tttctgacga actgaatacc
     7621 tgggtccgta gcggtccggg agatcattat cgcctcgtgg gcacggttaa cgccggcgag
     7681 gaagtgacct tattacaaac tgacgccaac accaattatg cccaggtgaa agacagctct
     7741 ggccgtaccg cctggatccc gttgaaacaa cttagcactg agccaagcct gcgctcccgt
     7801 gtgccagatc tggaaaatca ggtcaaaacc ctgaccgata aactcaccaa tatcgataac
     7861 acctggaatc agcgcaccgc agaaatgcag caaaaagtgg cgcagagcga cagcgtgatc
     7921 aacgggttaa aagaagaaaa tcagaaactg aaaaacgagc tgattgtcgc gcagaaaaag
     7981 gtcgatgccg ccagcgtaca gctggatgac aaacagcgca ccatcatcat gcaatggttt
     8041 atgtatggcg gtggcgtgct ggggcttggc ttgctgctcg gtctggtact gccgcacctg
     8101 atcccaagcc gcaaacgcaa agatcgctgg atgaactaaa tcgccttctt cgttgcactg
     8161 tcttattatt aggaattgtg tatttgacga agggatgatg gcgtgaagat ttatctggtc
     8221 ggtggtgctg ttcgggatgc attgttaggg ctatcggtca aagacagaga ttgggtggtg
     8281 gtcggcagta cgccacagga gatgctcgac gcgggctacc agcaggtagg ccgcgatttt
     8341 cctgtgtttc tgcatccgca aacgcatgaa gagtatgcgc tggcacgtac cgaacggaaa
     8401 tccggttccg gttacaccgg ttttacttgc tatgccgcac cggatgtcac gctggaagat
     8461 gatcttaagc gtcgcgatct gaccattaat gcgctggccc aggacgataa cggcgagatt
     8521 atcgacccgt acaacggtct gggcgatctg caaaatcgtc tgttgcgcca tgtttccccc
     8581 gcttttggcg aagatccgtt acgcgtactg cgcgtggcgc gttttgctgc gcgttatgcc
     8641 cacctcggtt ttcgtattgc cgatgaaact ctgacgctga tgcgcgagat gacccatgcg
     8701 ggtgaactgg aacacctgac gcctgaacgg gtatggaaag agacggaaag cgccctcacc
     8761 acccgcaatc cccaggtgtt cttccaggta ctgcgcgatt gcggcgcact tcgcgtttta
     8821 ttcccggaaa ttgacgcact gtttggcgtt ccggcacctg ccaagtggca tccggaaatc
     8881 gacacgggta ttcatacctt aatgacgctc tcaatggcgg cgatgctgag tccgcaggtt
     8941 gatgtccgtt tcgcaacttt atgccacgat ctcggtaaag gactgacgcc gccagaactt
     9001 tggccgcgtc atcatgggca tggcccggcg ggtgttaagt tagtggaaca actatgccag
     9061 cgtctgcgcg tgccaaatga aattcgcgat ttagccagac tggtggctga gtttcacgat
     9121 ctcatccaca ccttcccaat gctgaaccca aaaaccatcg tcaaattatt tgattccatc
     9181 gatgcctggc gtaaaccgca gcgtgtcgag caactggcgc tgaccagcga ggctgacgtg
     9241 cgcggcagaa ccggttttga atcagcggac tacccgcaag gccgttggtt gcgcgaaggc
     9301 tgggaagtgg cgcagtcagt gccgacaaaa gcggtcgttg aagcgggatt taaaggcgtg
     9361 gagattcgcg aggagctgac ccgacggcgg attgcggcgg tagccagctg gaaggaacaa
     9421 cgttgcccaa agcctgactg agtataaaac taaacgttag cacgaaccgt cccctcgccc
     9481 cttcggggag agggttaggg tgaggggaac agaccagcac cggtgcgaac aatcaccccc
     9541 accccggccc tctccctaca gagcgaggga gaaaacccgg cctgctcccg aaccttttct
     9601 caatcaaaag aacacgacat acaccgcagc cgccacaata aagcgataaa tggcgaacgg
     9661 gataaacgaa atgcgcttaa tcaattgcag gaaggtttta atcgctatca gcgccaccac
     9721 aaaagcggtg ataaacccaa cggcaaacat cgggatatcg ccggttgtca ggaagcccca
     9781 gcttttgtag agatcgagcg ccgttgcgcc catcatcatc ggtaccgcca gcaggaacga
     9841 gaactcggaa gcagcgtaac ggctcacgcc catcagcatc ccacctgaaa tggtcgcccc
     9901 ggaacgggag aaacccggcc acagcgccag acactggaaa cagccaatca taaacgcctg
     9961 acgataggtc atatcatcaa gacccggcgc acgcggctct ttcggcttca ggcattcggc
    10021 ggcaatcagc aacaaaccgc caacgaccag cgcatacatc acatttatcg ggttaaacaa
    10081 tgacttaatc gtgtcgtgga ataatagccc cagtaccacc gccggaatca tccccagcaa
    10141 aatgtggatc agcgttaaac gacctttgct ttcaccttcg tgctgcaacg ggcggccaaa
    10201 gtggatgcca atcaggccaa acagacgccg ccagaacatc actactaccg ccagaattga
    10261 tcctaactgg atcacaactt caaaggtttt cgccgtgtcg ccctcaaacc ccaacaagtg
    10321 accgacaata atcatatggc ccgtgctgga taccggcaga aattctgtca atccttcgac
    10381 cacacccaat attgccgcta tcagcagcga gtgcatatcg ctcatcaata aacccctaaa
    10441 ttattaaaat gtaccgcttg tccgaactac tgcagtatga ccaggttata accgtttggt
    10501 ttaacagctg taaaattaat tattttcttt cagattattg ccacgctcaa tgattacgcc
    10561 aacattcgcc gcccgcgcca ctgcgcctgg cttgctgagt ttgatacgca cccacggcga
    10621 gttgaagcgt gctaacagca gctccgccac ctcttcagcc acgcgttcca ccagcgcaaa
    10681 acgcgccccc tcgacgtggc tgaccaccgt ttctgcaatg tcagcgtaac tgaggcaatc
    10741 cgccacatca tcacttttcg ccgctttacg gttatcccat gccatttcga tatcgaacac
    10801 taacttctgt tcgatggtct gttcccagtc gtaaacacca atagtggtga ttaccgaaag
    10861 ttgctctata aatacaatat ccatcacgtc ctgcctgctt tttggctaac cggataccac
    10921 ttccggcgaa atgtgcgtat tatccacaga ttcatcgttg aacacgaatt ttcaaaacgg
    10981 aacagcttat gagtgcaatc gcgcctggaa tgatcctcat cgcgtacctc tgcggctcca
    11041 tttccagtgc cattctggtt tgccgcttgt gtgggctgcc cgatccgcga accagcggct
    11101 ccggcaatcc aggcgcaacc aatgtgttac gtatcggtgg caagggagca gccgtagcag
    11161 tactgatttt cgacgttctg aaaggaatgt tgcctgtctg gggcgcgtat gaattaggtg
    11221 tcagcccctt ctggctaggc ttaattgcca tcgccgcctg tcttggacac atctggcccg
    11281 ttttcttcgg ttttaaagga ggaaaaggcg ttgctaccgc ttttggagca atcgcaccaa
    11341 ttggctggga tctcaccgga gtaatggcgg gaacctggtt actgaccgtg ctattgagcg
    11401 gatactcgtc gctgggagcg attgtcagtg cactgattgc tccgttttat gtctggtggt
    11461 ttaagccaca attcaccttc ccggtttcga tgctctcttg cctgatcctg ctgcgtcatc
    11521 atgacaacat ccagcgtctg tggcgtcgtc aggagacaaa aatctggacg aaattcaaaa
    11581 gaaagcgcga aaaggatccc gagtgatttc tggtggatct acatgacctg atagccttca
    11641 tcgggcttgc ccagccgttg ctggcaccat gccgccagaa attccacgca gacacgaatt
    11701 tcatgctgcg atagagcggc tcccggtaaa cagcccagat attggcgctt tgtgcatact
    11761 ccggcaatac ttgcaccagt ttgccactct ccagaaacgg caacacatcc cactcggaac
    11821 gcagcataat ccctttgccc tccagcgccc attgcaacac tatctcgccg ctattggagg
    11881 aaagatgccc gctaaccttc accgattttt tctcctgacc gttccccaac tcccatattc
    11941 cgtgggtcat atcgcgctct ttggtcacca gacagtcatg acgacttaat tcctgtaatg
    12001 attgtggctg cggatatttt tgcagatact caggtgctgc gcataatatt cttttatttt
    12061 tcgttaacag atgcgcaata taataatcag gaatttcgtc attaatacga atatccagat
    12121 caatattatc ctgcactaaa tcaatttgcc gatcgaacaa ttcaaaatgc acctgtaact
    12181 caggataatt gcgcataagt tcggtaatgg ctggcgcaat atggctgcgc ccaaaaccaa
    12241 aactgcaacc aatacgaatc atcccctccg ggcgcgtttt gatttgcgtg acgtcatcga
    12301 ccagtcgctg atattgggtg aggatttcca gcgcatgttc gtaacaacgt tggccacttt
    12361 cagtcagcgc cacgccgcga gcggagcggt tcagcagcgt tgttgccagc gtattttcaa
    12421 ggatttggat ccgcttggtg acaaatgcag gcgtttgccc aagcgtcgcc gctgcagcac
    12481 taaaactccc gctgtggacg atttctacca gtacctgcaa gtctttggct aaaggccagc
    12541 tattcagcat cgttaattat ccgcagttgt gataagcgca gtgtatttcg caaaacattg
    12601 ccattccctg ctctggcgca atattcattc ttattttcgc gttgattcac gaactgttaa
    12661 ttacatattc acggtggcaa aaaatataaa accacatttt tagtggtagt ttgtggcggt
    12721 gaatttttcc agacaaatac aaaaactgga gttgccatga tgagcgaaag taataagcaa
    12781 caggcagtga ataagttgac agagattgtc gctaacttta ccgccatgat ttctacccga
    12841 atgcctgatg acgtggtgga taaactaaaa cagctaaagg atgccgaaac gtcgtcgatg
    12901 gggaaaatta tctaccatac gatgttcgac aacatgcaaa aagcgattga cctgaatcgt
    12961 cctgcctgtc aggacaccgg ggagattatg ttcttcgtta aagtcggttc ccgcttccca
    13021 ctgcttggcg agctgcaaag catactcaaa caagccgtgg aagaggcaac cgtcaaagcg
    13081 ccactacgtc acaatgcggt agaaattttt gacgaagtaa acaccggcaa aaataccggt
    13141 agcggcgtac cgtgggtcac ctgggacatc atccccgaca atgacgatgc ggaaatcgaa
    13201 gtttacatgg caggcggcgg ctgcacgcta cctggccgct cgaaagtgtt aatgccgtca
    13261 gaaggctacg aaggcgtggt gaaattcgtc ttcgaaaata tctccaccct cgccgtaaac
    13321 gcctgtccac cggtactggt gggcgtgggc atcgccacct cggtggaaac cgccgccgta
    13381 ctctcgcgta aagccattct gcgcccgatt ggcagccgcc atcccaatcc aaaagcggca
    13441 gaactggagc tacgcctgga agaaggactc aaccgtctgg ggattggtcc acaagggctg
    13501 accggcaaca gttcagtgat gggcgcatcg tcgcggcacg ctgctggttc atgccgatct
    13561 cacctttgaa aatctgtctc acacccggag cgcgttatga aaaagatcct gacaaccccg
    13621 atcaaagctg aagatctgca agatattcgc gtcggcgatg tgatctacct gaccggtacg
    13681 ctggtgacct gccgcgacgt ttgtcaccgc cgtttgatcg aactgaaacg tccgatccct
    13741 tacgatctca acggcaaagc gattttccac gctggcccca tcgtgcgcaa aaacggcgac
    13801 aaatgggaga tggtctccgt cggcccgaca accagtatgc gtatggaaag ttttgaacgt
    13861 gaatttattg agcagaccgg cgtaaaactg gtggttggca aaggtggtat ggggccgctg
    13921 accgaagaag gctgccagaa attcaaggcg ttacatgtga ttttcccggc aggctgcgcg
    13981 gtgctggcgg caacccaggt agaagagatt gaagaagtgc actggacaga actcgggatg
    14041 ccggagtcac tgtgggtctg ccgggtcaaa gagttcggcc cgctgattgt ctctattgat
    14101 acccacggca acaacctgat agccgaaaac aaaaagctgt tcgccgaacg ccgcgatccc
    14161 atcgtggaag agatctgcga gcatgtccat tacatcaaat aaccctcccg gagaggctca
    14221 cccctctcct ttttcgcagg cataacacga tgaaaccttc cactgaatgg tggcgatatc
    14281 ttgcaccgct ggcggtcatc gccattattg ctctacttcc ggttcccgca gggctggaga
    14341 accatacctg gctctacttt gccgttttta ctggcgtgat cgttggactg atcctcgaac
    14401 ccgtgccggg tgccgtggtg gcgatggtgg gtatctccat catcgccata ctctctccct
    14461 ggctgctgtt cagccccgaa cagctcgccc agccaggctt taagttcacc gccaaatccc
    14521 tctcctgggc agtttctggt ttttctaatt cggttatctg gctcattttc gccgccttta
    14581 tgtttggcac aggctatgaa aaaaccgggc ttggacgccg cattgcgctg attctggtga
    14641 aaaagatggg gcatcgcacg ctgtttctcg gctatgcggt gatgttctcc gagctgatcc
    14701 tggcacctgt aacaccgtcc aactcggcgc gtggtgcggg tattatctat cccatcatcc
    14761 gtaacctgcc accgctctat caatcacaac caaacgacag cagttcgcgc agcattggct
    14821 cgtacatcat gtggatgggg attgttgccg actgcgtgac cagcgccatt ttcctgacgg
    14881 cgatggcacc taacttgctg ttaattggac tgatgaaaag cgcatctcac gccacgctga
    14941 gttggggcga ctggttcctc gggatgttgc cgctcagcat tttactgctt ctgctggttc
    15001 cctggctggc ttacgtgcta tacccgccgg tactgaagtc tggtgatcag gtgccgcgct
    15061 gggcagagac ggaactgcag gcaatgggcc cgctctgttc gcgtgaaaaa cggatgctgg
    15121 ggctgatggt aggcgcgctg atgctgcttc tgcgcattat cagttgggac gacattgtca
    15181 gtaataaagc ggcatggaac gttttcttct ggctggcctc gcttatcacc ctcgcgaccg
    15241 gactcaacaa taccggtttt attagctggt ttggcaaact gttagcaggc agcttaagtg
    15301 gttattcgcc gacgatggtg atggtgacgt tgattgtggt gttttatcta ctgcgctact
    15361 ttttcgccag cgccacggcg tatacctccg ccctcgcacc gatgatgatc gccgccgcgc
    15421 tggcgatgcc ggaaatcccg ctgccggtgt tctgcctgat ggttggcgcg gcaattggtc
    15481 tggggagcat tcttacacca tacgccaccg gacccagtcc gatttactac ggtagtggtt
    15541 atctgccaac ggcggattac tggcgactgg gggcgatttt tgggctgata ttcctcgtgt
    15601 tgctggtgat taccggctta ctgtggatgc ccgtggtgtt gctttaagtg aggacggtgc
    15661 cggaaattgc tctccggcac ccaatagttt tacgcagccg gtaactccgc cagcggccag
    15721 cgcggacgca cgctaacgcc gagatccgcc gtcgcgcctg ctttaaaccg caccattccg
    15781 gcataggcga tcatcgcgcc gttatcagta caaaattccg gacgcgcgta gaacacttcg
    15841 ccgcggcgtt ttttcatcat ttcagccagc ttcgcccgta acgtgcggtt agcactcacg
    15901 ccgcccgcca tgaccagtcg cttaaagccc gtctgatcca acgctcgctt acacttaatc
    15961 atcaacgtat cgaccaccgc atcttcaaag gcgcgggcga tatcagcacg cgtctggtcg
    16021 tcggtgctgt tgtcacgaat cgtgttcgcc gcaaaggttt tcagaccaga aaagctgaaa
    16081 tccagccccg gacggtcggt catcggacgc gggaaaacaa agcgcccggc agtaccctga
    16141 gccgccattt tcgacagtaa cggtccgcca ggataatcca gccccagcag cttcgcggtt
    16201 ttatcaaacg cttcaccggc ggcatcatcg atagactcgc cgagcagctc gtactgacca
    16261 atgccagtca cgctgattaa ctgcgtatga ccgccggaca ccagcagcgc gacaaacgga
    16321 aattccggcg ggttatcttc cagcatcggc gctaacagat gcccttccat atggtgtacc
    16381 gggattgccg gaacgttcca ggcaaacgcc agagagcgcc ccacggttgc gccaaccagc
    16441 agtgcgccga ctaatccagg gcctgcggta taggccacag catcaatgtc ttttgccgtt
    16501 aaaccagact cctttagcgc cgcctggatc aacggtacgg ttttacgcac atgatcgcgg
    16561 gaggccagtt caggcacgac gccgccgtag tcagcgtgca atttcacctg actatacaat
    16621 tggttggcta acaaaccttt ttcatcgtcg taaatggcga tgccggtttc atcgcaggaa
    16681 gtttcaatac ccagtacacg catgacttgt tttacctcgc tttattaccg cgcagtgtag
    16741 gaccaatgcg ggttgatgta aaactttgtt cgcccctgga gaaagcctcg tgtatactcc
    16801 tcacccttat aaaagtccct ttcaaaaaag gccgcggtgc tttacaaagc agcagcaatt
    16861 gcagtaaaat tccgcaccat tttgaaataa gctggcgttg atgccagcgg caaaccgaat
    16921 taatcaaagg tgagaggcac atgccggtaa ttaaagtacg tgaaaacgag ccgttcgacg
    16981 tagctctgcg tcgcttcaag cgttcctgcg aaaaagcagg tgttctggcg gaagttcgtc
    17041 gtcgtgagtt ctatgaaaaa ccgactaccg aacgtaagcg cgctaaagct tctgcagtga
    17101 aacgtcacgc gaagaaactg gctcgcgaaa acgcacgccg cactcgtctg tactaattcc
    17161 ccgagagcgt tgctctccga tcagaccgag ttgtagttgt aaggccgtgc ttccgaaagg
    17221 aatgcgcggc ttattttcgt ttatgaattg ctaaaaatcg gggcctatgg ctggacgaat
    17281 cccacgcgta ttcattaatg atctgctggc acgcactgac atcgtcgatc tgatcgatgc
    17341 ccgtgtgaag ctgaaaaagc agggcaagaa tttccacgcg tgttgtccat tccacaacga
    17401 gaaaaccccg tcattcaccg ttaacggtga gaaacagttt taccactgct ttggatgtgg
    17461 cgcgcacggc aacgcgatcg acttcctgat gaactacgac aagctcgagt tcgtcgaaac
    17521 ggtcgaagag ctggcagcaa tgcacaatct tgaagtgcca tttgaagcag gcagcggccc
    17581 cagccagatc gagcgccatc agaggcaaac gctttatcag ttgatggacg gtctgaatac
    17641 gttttaccaa caatctttac aacaacctgt tgccacgtct gcgcgccagt atctggaaaa
    17701 acgcggatta agccacgagg ttatcgctcg ctttgcgatt ggttttgcgc cccccggctg
    17761 ggacaacgtc ctgaagcggt ttggcggcaa tccagaaaat cgccagtcat tgattgatgc
    17821 ggggatgttg gtcactaacg atcagggacg cagttacgat cgtttccgcg aacgggtgat
    17881 attccccatt cgcgataaac gcggtcgggt gattggtttt ggcgggcgcg tgctgggcaa
    17941 cgataccccc aaatacctga actcgccgga aacagacatt ttccataaag gccgccagct
    18001 ttacggtctt tatgaagcgc agcaggataa cgctgaaccc aatcgtctgc ttgtggtcga
    18061 aggctatatg gacgtggtgg cgctggcgca atacggcatt aattacgccg ttgcgtcgtt
    18121 aggtacgtca accaccgccg atcacataca actgttgttc cgcgcgacca acaatgtcat
    18181 ttgctgttat gacggcgacc gtgcaggccg cgatgccgca tggcgagcgc tggaaacggc
    18241 gctgccttac atgacagacg gccgtcagct acgctttatg tttttgccgg atggcgaaga
    18301 ccctgacacg ctggtacgaa aagaaggtaa agaagcgttt gaagcgcgga tggagcaggc
    18361 gatgccactc tccgcatttc tgtttaacag tctgatgccg caagttgatc tgagtacccc
    18421 tgacgggcgc gcacgtttga gtacgctggc actgccattg atatcgcaag tgccgggcga
    18481 aacgctgcga atatatcttc gtcaggaatt aggcaacaaa ttaggcatac ttgatgacag
    18541 ccagcttgaa cgattaatgc caaaagcggc agagagcggc gtttctcgcc ctgttccgca
    18601 gctaaaacgc acgaccatgc gtatacttat agggttgctg gtgcaaaatc cagaattagc
    18661 gacgttggtc ccgccgcttg agaatctgga tgaaaataag ctccctggac ttggcttatt
    18721 cagagaactg gtcaacactt gtctctccca gccaggtctg acctccgggc aacttttaga
    18781 gcactatcgt ggtacaaata atgctgccac ccttgaaaaa ctgtcgatgt gggacgatat
    18841 agcagataag aatattgctg agcaaacctt caccgactca ctcaaccata tgtttgattc
    18901 gctgcttgaa ctgcgccagg aagagttaat cgctcgtgag cgcacgcatg gtttaagcaa
    18961 cgaagaacgc ctggagctct ggacattaaa ccaggagctg gcgaaaaagt gatttaacgg
    19021 cttaagtgcc gaagagcgat cgggaagccc ccgacagccg cactgagagg cagcggcaaa
    19081 tatataagta cgccctcgta attatcgttg gcggtaaaca accgttggat ttcagcgtta
    19141 acggctgaag gacatcgggt caatcgccca acaccaacct catgaaataa gtgtggatac
    19201 cgtcttatgg agcaaaaccc gcagtcacag ctgaaacttc ttgtcacccg tggtaaggag
    19261 caaggctatc tgacctatgc cgaggtcaat gaccatctgc cggaagatat cgtcgattca
    19321 gatcagatcg aagacatcat ccaaatgatc aacgacatgg gcattcaggt gatggaagaa
    19381 gcaccggatg ccgatgatct gatgctggct gaaaacaccg cggacgaaga tgctgccgaa
    19441 gccgccgcgc aggtgctttc cagcgtggaa tctgaaatcg ggcgcacgac tgacccggta
    19501 cgcatgtaca tgcgtgaaat gggcaccgtt gaactgttga cccgcgaagg cgaaattgac
    19561 atcgctaagc gtattgaaga cgggatcaac caagttcaat gctccgttgc tgaatatccg
    19621 gaagcgatca cctatctgct ggaacagtac gatcgtgttg aagcagaaga agcgcgtctg
    19681 tccgatctga tcaccggctt tgttgacccg aacgcagaag aagatctggc acctaccgcc
    19741 actcacgtcg gttctgagct ttcccaggaa gatctggacg atgacgaaga tgaagacgaa
    19801 gaagatggcg atgacgacag cgccgatgat gacaacagca tcgacccgga actggctcgc
    19861 gaaaaatttg cggaactacg cgctcagtac gttgtaacgc gtgacaccat caaagcgaaa
    19921 ggtcgcagtc acgctgccgc tcaggaagag atcctgaaac tgtctgaagt attcaaacag
    19981 ttccgcctgg tgccgaagca gtttgactac ctggtcaaca gcatgcgcgt catgatggac
    20041 cgcgttcgta cgcaagaacg tctgatcatg aagctctgcg ttgagcagtg caaaatgccg
    20101 aagaaaaact tcattaccct gtttaccggc aacgaaacca gcgatacctg gttcaacgcg
    20161 gcaattgcga tgaacaagcc gtggtcggaa aaactgcacg atgtctctga agaagtgcat
    20221 cgcgccctgc aaaaactgca gcagattgaa gaagaaaccg gcctgaccat cgagcaggtt
    20281 aaagatatca accgtcgtat gtccatcggt gaagcgaaag cccgccgtgc gaagaaagag
    20341 atggttgaag cgaacttacg tctggttatt tctatcgcca agaaatacac caaccgtggc
    20401 ttgcagttcc ttgacctgat tcaggaaggt aacatcggcc tgatgaaagc ggttgataaa
    20461 ttcgaatacc gccgtggtta caagttctcc acctacgcaa cctggtggat ccgtcaggcg
    20521 atcacccgtt ctatcgcgga tcaggcgcgc accatccgta ttccggtgca tatgattgag
    20581 actatcaaca aactcaaccg tatttctcgc cagatgctgc aagagatggg ccgtgaaccg
    20641 acgccggaag aactggctga acgtatgctg atgccggaag acaagatccg caaagtgctg
    20701 aagatcgcca aagagccaat ctccatggaa acgccgatcg gtgatgatga agattcgcat
    20761 ctgggggatt tcatcgagga taccaccctc gagctgccgc tggattctgc gaccaccgaa
    20821 agcctgcgtg cggcaacgca cgacgtgctg gctggcctga ccgcgcgtga agcaaaagtt
    20881 ctgcgtatgc gtttcggtat cgatatgaac accgaccaca cgctggaaga agtgggtaaa
    20941 cagttcgacg ttacccgcga acgtatccgt cagatcgaag cgaaggcgct gcgcaaactg
    21001 cgtcacccga gccgttctga agtgttgcgt agcttcctgg acgattaatc ggtaggccgg
    21061 atcaggcgtt acgccgcacc cggcactagt ctctctgcac aaacgccacc ttt
//
LOCUS       DAJVQH010000066        20980 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJVQH010000066 DAJVQH010000000
VERSION     DAJVQH010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.066"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(1..3035)
                     /locus_tag="OQM87_002442"
                     /pseudo
     CDS             complement(1..3035)
                     /locus_tag="OQM87_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            complement(3042..3215)
                     /locus_tag="OQM87_002443"
     CDS             complement(3042..3215)
                     /locus_tag="OQM87_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508544.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            3333..4526
                     /locus_tag="OQM87_002444"
     CDS             3333..4526
                     /locus_tag="OQM87_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCS1508545.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4724..5977)
                     /gene="glyA"
                     /locus_tag="OQM87_002445"
     CDS             complement(4724..5977)
                     /gene="glyA"
                     /locus_tag="OQM87_002445"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCS1508546.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6304..7494
                     /gene="hmpA"
                     /locus_tag="OQM87_002446"
     CDS             6304..7494
                     /gene="hmpA"
                     /locus_tag="OQM87_002446"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCS1508547.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7539..7877)
                     /gene="glnB"
                     /locus_tag="OQM87_002447"
     CDS             complement(7539..7877)
                     /gene="glnB"
                     /locus_tag="OQM87_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCS1508548.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7938..9272)
                     /gene="glrR"
                     /locus_tag="OQM87_002448"
     CDS             complement(7938..9272)
                     /gene="glrR"
                     /locus_tag="OQM87_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCS1508549.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9262..9975)
                     /gene="qseG"
                     /locus_tag="OQM87_002449"
     CDS             complement(9262..9975)
                     /gene="qseG"
                     /locus_tag="OQM87_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCS1508550.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10140..11567)
                     /gene="qseE"
                     /locus_tag="OQM87_002450"
     CDS             complement(10140..11567)
                     /gene="qseE"
                     /locus_tag="OQM87_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCS1508551.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12125..16012)
                     /gene="purL"
                     /locus_tag="OQM87_002451"
                     /gene_synonym="purI"
     CDS             complement(12125..16012)
                     /gene="purL"
                     /locus_tag="OQM87_002451"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCS1508552.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16270..17826
                     /gene="mltF"
                     /locus_tag="OQM87_002452"
     CDS             16270..17826
                     /gene="mltF"
                     /locus_tag="OQM87_002452"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCS1508553.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17823..18359)
                     /gene="tadA"
                     /locus_tag="OQM87_002453"
     CDS             complement(17823..18359)
                     /gene="tadA"
                     /locus_tag="OQM87_002453"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCS1508554.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18384..19019)
                     /gene="yfhb"
                     /locus_tag="OQM87_002454"
     CDS             complement(18384..19019)
                     /gene="yfhb"
                     /locus_tag="OQM87_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCS1508555.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19228..20076
                     /locus_tag="OQM87_002455"
     CDS             19228..20076
                     /locus_tag="OQM87_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCS1508556.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(20415..20969)
                     /gene="pinE"
                     /locus_tag="OQM87_002456"
                     /pseudo
     CDS             complement(20415..20969)
                     /gene="pinE"
                     /locus_tag="OQM87_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT         5034 a         5673 c         5341 g         4932 t
ORIGIN      
        1 ggccggatta atcgccgtca attcagcgcg agcagactca tattgcgcaa cctccccgag
       61 tcccgcggac gccagcatcg ccaccatcag gcatttttct ttatgctgct gttgtaaatc
      121 gccatacagc gataacaggt caggttgtga aacagcaaag aaatccgcct caatactggt
      181 tttcgccatc tcttgcgccc actgtttcat ttcactaaac agttgctgtg cggtttgctg
      241 ttcacccagc agtcgcagcg ccattccttg ccagaagaga taatcaaccg gctgatcgtt
      301 gtaataactg tggatgttaa tggtgcgatc gccggtcgcc gccagacgta aacaacgcat
      361 cgcttcagtt tcatcgccct gcgcgttggc gcatatcgcc tgccagaacc agatgtcgtt
      421 atcagtttgc cccggtaaac ggccttcgct taaattctcc ggataatgca gcgcggcatg
      481 aagcagttcg ctggcctgct gcggctctct ggcatcaaga tgctgccagg cgcgtaataa
      541 ctggttgagg ataaactgac tggtgacctt cccttccccg ccttcccacg ggtggaattt
      601 acgcgtggcg agaatgtccg ccgctttgtc tgcctgaccc gtgagatgcc acaaattgag
      661 cagttctgcg gtcatgtcgt cgcgtttcag cgcaatttcc agattatttt ccagacgcgc
      721 cagtcgtttc tccggtgtgg ctccacttaa cttatccagc aaatcccgtt cgaaaagcag
      781 acgtgcatcc tgcggcgcaa gctgataagc attatcaaga taacgcgcgg ccagctcata
      841 atcgtgtttc ttattccacg catggatcgc taacccgcgc cagccgtcgg caaactccgg
      901 cgacatctct acgcaacgtt gccataaggc aatggctttg ttgtagctac gtttgttgta
      961 gtagaagcag gccagtaaat ggcgagcaaa ccagcactct tcaatactct ccagcgccgc
     1021 cacttcttcc agcgtattcg ggaaacggac aaactgcggg aagacatcaa tggcttttgc
     1081 gaccagttcg ccacgttcgg ctttcggcag caagctggct tgcaggtaaa gcggcagcgt
     1141 gcgctggcag tccagtgcgt tcagcatctc tgccgccaga gtgggcattc cccagttaat
     1201 cagttgcccg gcggtcatca gagcgttaac gtcgcgtccc tgacacagac cgcgccactg
     1261 cgcgagggct gattcgctac gaccatcgaa ccagttcagc caccacagag tggcgttcag
     1321 cggataatca cgcagcagtt tctcgcgctg caaacgcgcg ttgtcctgac gaccacttaa
     1381 caccagcagc agattatgca agcaaagcac ttcctgattg gttgggcagg cacgaagact
     1441 ttgttggcaa aaatccagac ccgcgtcgaa gttatcatta cgcgccgcca gtcgtgccag
     1501 accataatag ccgcctgctt tactattgcc gctccagacc gcacgccaga aatcctcttc
     1561 ggcttgttga tattgtccct gacgttcgta agcactggcg cgaatcaaac ttgcttgtcc
     1621 acactgcggg tttttgttca gcgcatgtgc gcgtcctgac tggcatacgc caccgcttgc
     1681 gggaaatctg cgcggttata ttccagcatc gccagcgcca ggttacagcg ataatccagc
     1741 gggtccagcg ccacgccgcg caggtagtaa tcgaacggtg aacggctggc gtgatgatat
     1801 tgctccagat gctgaccgat aaaccaggct tcatctgtac tggtaatgtc ttgtgctgcc
     1861 agtggcgctt tggcgacgtc cggcaacggc aactcctgcg gctgatgttc ctgataactc
     1921 agtacaatat tgccgtcggc atcggagagt tcaatggtca gtctgtcagg attgataccg
     1981 tgcaacacgc cctggatggc ggtcgctggt gtcagggcca cggcatcatc gagcaacgcg
     2041 ttgcatttgc cgatttcgcg gatcgccaga cggtatccgt tcaacggaga gatggcatac
     2101 agcccccact caatcccccg ctcactacgc tggagtttta tcaccgcatc gcgggaggca
     2161 ttttgcacca tgcacagaga atgataaggc aggaaatact gctcgaaacg cttctcttcg
     2221 taagcatcaa gccaggtaaa atcaggctgg ttatcggcaa aaataccggt catcagttcg
     2281 atatacgggc cgttattgtc ggtcagactc ttatcccacg cctggccaaa ttcactgtgt
     2341 ccccaactcc actgtttttt acctggcgca atatggtggt tggcaacgtg cagcaaaccg
     2401 ccatcttcat cgtgacacca cgcgccgaca aaatcgtact gcgatttttc ggccatatat
     2461 gaggttggaa caggcacatt tttatagcga gaaatgtcca ctccggcgga gtagtccact
     2521 ttgtagtaag tgccggtggc gatggggaaa gcggagacgg cccgtttgcc gtgatcaaac
     2581 accgcagtca catccggtgg gaaaacgctc tgatgccctt cacccccttt cactgccggg
     2641 ttggcccacc acaagaaatg acgcggcgtg gcgttgccgt tatagacgcg gctggcgatt
     2701 tccagtgccg cccggtcagg gcgcagggtg aaacctgtca tcacctgtaa accatgcatc
     2761 ggctccgttt cgccgaccca caccgtctgt gcaccgtctt catgggcttc gagggtgaaa
     2821 tcaacgggca taaaggtggt cgggcgatgg tgttgcggcc agttaaactc aatcccgccg
     2881 gagatccacg gtcccagcag ccccaccagc gcaggtttaa tgacttcatt gtgatagaca
     2941 aaatcgcgct gtttcacttt atcccatgcg cgatgcaccc gaccgcccag ttccggcagg
     3001 atcatcactt tgatgtagtc gttttccagc cacaccgcct gctaggattt cagggttttc
     3061 tgctcgctca gcgtatcggt cacgccgtag ggataaaccg cgccggacga tccctgataa
     3121 acgcgatttt ccaggaacat gggatgtata tcctgcggcc cggtttcata ggtcgggatc
     3181 tcaacgcgct cttgccacac ttttactgga gtcatggtgc cctcaatatt aacaagacat
     3241 actgaattaa aagatttgtg gcagtgtatt gaacaatctg gcaatgtttt cgcggaataa
     3301 tcacgcaatt aactaaacaa ggtttagtga agatgagagc ctgcattaac aatcaacaga
     3361 ttcgccacca taacaaatgc gtgattctgg aactgctgta ccggcaaaag cgcgccaata
     3421 aatcaacgct ggcccggctg gcgcaaattt cgattccggc agtcagtaat attttgcagg
     3481 aactggaaag cgaaaaacgg gtggtgaata ttgacgatga aagccagacg cgcgggcata
     3541 gtagcggtac atggctgatt gcgccggaag gtgactggac gctgtgcctg aacgtgacgc
     3601 ccaccagtat tgagtgtcag gttgctaatg cttgtttaag tccgaaaggc gaatttgagt
     3661 atttacagat tgatgtaccg acgccgcagg cgctgctgtc cgaaatcgaa aaatgctggc
     3721 atcgccaccg taaattgtgg ccggaccgca ccatcaatct ggcgctggca atccacgggc
     3781 aggttgatcc ggtgaccggc gtatcgcaaa ccatgccgca agcgccgtgg acaacgccag
     3841 ttgaggtgaa gtatctgctg gaagagaagc tgggcattcg ggtgctggtc gataatgact
     3901 gcgtgatgct ggcgcaggcg gagaaatggc aaaataattc gcaggaacgg gatttctgcg
     3961 tgatcaacgt tgattacggc attggctcgt cgttcgtgat taacgagcaa atttatcgcg
     4021 gcagtttgta tggtagcgga cagattggtc acaccatcgt taatccggat ggcgtcgtct
     4081 gcgactgtgg acgttacggc tgcctggaaa ccgtcgcctc gttaagtgca ttaaaaaaac
     4141 aggcgcgggt atggctaaaa tcacaaccgg ttaatactca gcttgatcct gaaaaactga
     4201 caaccgcgca gttaatcgct gcctggcaga gtggagaacc gtggatcacc agctgggttg
     4261 atcgctctgc caatgccatt ggtttgagtc tgtataactt cctcaacatc ctcaatatta
     4321 atcagatttg gttgtacggt cgcagttgtg cctttggtga gaactggctt aatactatta
     4381 ttcgccagac aggatttaac ccgttcgacc gcgacgaagg accgagcgtg aaagcgacgc
     4441 aaattggctt cgggcaatta agccgcgcac aacaggtgct gggaattggc tatttgtatg
     4501 ttgaggcgca gttacgacag atttgatggc gcgataacgt agaaaggctt cccgaaggaa
     4561 gccttgatga tcataaacga aaaattgcct gatgcgctac gcttatcagg cctacacgga
     4621 gattgcaata tattgaattt gcaagatttt gtaggcctga gaaggtgttc atgccgcatc
     4681 caacatgaac aacgagcaca ttgtcagcaa atcaccgttt cgcttatgcg taaaccgggt
     4741 aacgtgcgca gatgtcgaga actttacctt tgatgcgctc gataacggct tcatcattga
     4801 tgctgtccag cacgtcacac atccagccag ccagttcttt cgcttcagct tctttaaagc
     4861 cgcgacgggt aatcgccgga gtacctacac gaataccgga ggtcacaaac gggctcttcg
     4921 gatcgttcgg tacgctgttt ttgttgacgg tgatgttagc acggcccaga gcggcgtctg
     4981 cttctttacc ggtcaggttt ttatcaacca gatcaaccag gaacaggtgg ttatcagtgc
     5041 cgccggaaac cactttgtag ccgcgctcga ggaacacttc taccatcgct ttagcgtttt
     5101 tcgcgacctg ctgctggtaa gttttgaact caggctccat cgcttctttc agagcaaccg
     5161 ctttaccggc gattacgtgc atcaacggac cgccctgacc accagggaaa acggcagagt
     5221 tcagtttttt gtacagctct tcgctaccac ctttcgccag gatcaggccg ccgcgcggac
     5281 ccgccagggt tttgtgagtg gtggtagtaa caacgtgagc atgaggaacc gggttcgggt
     5341 agacgccagc agcaaccagg cccgcaacgt gcgccatatc aacgaacaga taagcaccga
     5401 tgctgtcagc gatttcacgc attttcgccc agtcaaccac gccggaatat gcagagaaac
     5461 caccgataat cattttcggc ttgtgttctt tggcttgttt ttccagatcg gcgtaatcga
     5521 tatgaccggt agcatcgata ccgtaaggaa cgatgttgta cagtttaccg gagaagttaa
     5581 ccggagaacc gtgagtcagg tgaccgccat gcgccaggtt catacccaga acggtatcac
     5641 ctggttccag cagcgcggtg tagaccgcaa agttagcctg ggagccggag tgcggctgga
     5701 cgttagcgta gtcagcgccg aacagttctt tcgcacgatc gatcgccagt tgttcaacga
     5761 tatcaacata ctcgcaaccg ccgtagtagc gtttgcccgg ataaccttca gcatatttgt
     5821 tggtcagctg agaaccctgc gcctgcatta cgcgcgggct ggtgtagttt tcggaggcga
     5881 tcagttcgat gtgctcttcc tgacgtactt tttcctgctc catagcctgc cacagttcgg
     5941 catcataatc ggcaatgttc atttcacgct ttaacatccg catctcctga ctcagctaac
     6001 aataaaattt ttggccttta taggcggtcc tgttggacaa cggcgaacag tataaccgaa
     6061 tcattgtgcg ataacaggtc ttgacaaagg aatttacgca aacgattacc ttaaggctac
     6121 gcaaggcttt ggagaataaa gagcttgcaa ccggaaacgg atttcttttc aggtttgtga
     6181 tgcaaatttt tcacttcatc acattctttc tgaaaaacac caaagaacca tttacattgc
     6241 agggctattt tttataagat gcatttgaga tacatcaatt aagatgcaaa aaaggaagac
     6301 catatgcttg acgctcaaac catcgctaca gtaaaagcca ccatcccttt actggtggaa
     6361 acggggccaa agttaaccgc ccatttctac gaccgtatgt ttactcataa cccagaactc
     6421 aaagaaattt ttaacatgag taaccagcgt aatggcgatc aacgtgaagc cctgtttaac
     6481 gctattgccg cctacgccag taatattgaa aacctgcctg cgctgctgcc agcggtagaa
     6541 aaaatcgcgc agaagcacac cagcttccag atcaaaccgg aacagtacaa catcgtcggt
     6601 gaacacctgt tggcaacgct ggacgaaatg ttcagcccgg ggcaggaagt gctggacgcg
     6661 tggggtaaag cctatggtgt actggctaat gtatttatca atcgcgaggc ggaaatctat
     6721 aacgaaaacg ccagcaaagc cggtggttgg gaaggtactc gcgatttccg cattgtggct
     6781 aaaacaccgc gcagcgcgct tatcaccagc ttcgaactgg agccggtcga cggtggcgca
     6841 gtggcagaat accgtccggg gcaatatctc ggcgtctggc tgaagccgga aggtttcccg
     6901 catcaggaaa ttcgtcagta ctctttgact cgcaaaccgg atggcaaagg ctatcgtatc
     6961 gcggtgaaac gcgaagaggg tgggcaggta tccaactggt tgcacaatca cgccaatgtt
     7021 ggcgatgtcg tgaaactggt cgctccagca ggtgatttct ttatggctgt cgcagatgac
     7081 acaccagtga cgttaatctc taccggtgtt ggtcaaacgc caatgctggc aatgctcgac
     7141 acgctggcaa aagcaggcca cacagcacaa gtgaactggt tccatgcggc agaaaatggc
     7201 gaagttcacg cctttgctga tgaagttaag gaactggggc agtcactgcc gcgctttacc
     7261 gcgcacacct ggtatcgtca gccgagcgaa gccgatcgcg ctaaaggtca gtttgatagc
     7321 gaaggtctga tggatttgag caaactggaa ggtgcgttca gcgatccgac aatgcagttc
     7381 tatctctgcg gcccggttgg cttcatgcag tttaccgcga aacagttagt ggatctgggc
     7441 gtgaagcagg aaaacattca ttacgaatgc tttggcccgc ataaggtgct gtaatttgat
     7501 gttgccggat ggaaacatcc ggcaaccctt gacgcggttt aaattgccgc gtcgtcctct
     7561 tcaccggtac ggatgcgaat gacccgagcc acgtcaaaga cgaagatttt accgtcaccg
     7621 attttgccgg tttgcgccgt gcgaataatg gtatcgacac aggtatcgac aatgtcgtcc
     7681 ggtacgacaa tctcaatttt cactttcggc agaaaatcca ccatatactc cgcgccgcgg
     7741 tacagctcgg tatggccttt ctggcgacca aagcctttca cttcggtcac cgtcatgccg
     7801 gtaataccga cttcggccag tgcttcgcgg acatcgtcca gcttgaaggg ttttataatc
     7861 gcatcaatct ttttcatgct attccttgaa aaggttgtct gtcttttgat ctgctaaacg
     7921 taacacataa cgccaattca ttccttgaaa tcgtttgcat ccagctcgtg tcgggaaagc
     7981 agtttataaa attctgtccg gttgcgcccc gccattctcg ccgcgtgggt gacgttgcct
     8041 ttggtgattt gcagcagctt acgcaaatag ttgagttcaa aatgattacg tgcctcaaca
     8101 aaggttggca gcgccgtatt ttcaccctcc agcgcctgct ccaccagcgc atcactaatc
     8161 accggagatg aggtcagcgc cacgcactgt tcaatcacgt tgaccaactg gcgcacatta
     8221 cccggccagc tcgcggtcat caggcgtttc atcgcatcgg tagagaacgc gcggacaaac
     8281 ggtttatgtc gctctgccgc ctggcgcaac aggtgatttg ccagtagcgg aatgtcttct
     8341 gtgcgctccg ccagtgccgg aattttcagg ctgacaacgt tgaggcggta atagagatct
     8401 tcgcggaatt ccccgcgcgc catcgctttt gacagatcac ggtgagtggc agaaataatc
     8461 cgcacattga tatcaatatc gcggttactg cccagcgggc gcactttacg ctcctgcaac
     8521 acgcgcagca gtttgacctg taacggcgca ggcatatcgc caatttcatc gagaaatagc
     8581 gttccgcctt ccgccgcctg gaataaacct tcgcgattgc tgacagcgcc agtaaacgcg
     8641 ccacgcgcat gaccaaacag ctccgactcc agcaattgct cgggcaatgc gccacagtta
     8701 atagcaataa atggtttgct gttgcgcggg ctggcgttgt ggatagcctg ggcgaaaatc
     8761 tctttcccgg tgccgctctg accgttaatc aaaacgctga cgtctgattg cgccaccagc
     8821 cgcgcctgtt ccagcaaacg cagcatcagc gggctgcggg tgacaattgc ctcgcgccag
     8881 cgttcatcgg tggctggcgc ggattgctcc agcgcatcgt caattgcctg atatagcgca
     8941 tctttatcga caggcttggt gaggaaacta aaaacgccct gctgtgttgc agcaacggca
     9001 tcggggatag aaccatgcgc agtaagaata attactggca ttcccggctg cactttctgg
     9061 atttcagcaa atagctgcat accgtccatt tcatccatcc gcaggtcgct gataactaaa
     9121 tctacttttt cgcgattcag tacccgtaat ccttcagcgc cactttccgc cgtgaccaca
     9181 ctgtagcctt cgctggtcag gcgcaggcca agcagtttca gcaatcccgg atcgtcatcg
     9241 accaataata aatgcgcagg tttatggctc atcaggagtg acctcatggg tggatggcgc
     9301 gggcttttca ctctcgtgcg gcgtatccgg cgagaaattt ccagccggtt tgcgggtcga
     9361 gagctggcgt tcaatatcgg tcaggttttc cagcttgcgg gtggtgagtt ccagttgctg
     9421 ctgtagaacg tgatgttgct ggcgcaatgt atccagctcg ctgtcgctgg actgctggag
     9481 tttactgtaa cgttggcgct cttccgccag ttgcagttgc agcgcctgac cgtcgcgcca
     9541 gagttgatac agtgggcgaa cctgtgccgg gatctcggta cttaacgctt caatacgcgc
     9601 gaccagctgg cggcgctcat acggcgtaat tttggcgtcg gcgagcaaaa tcccttgttt
     9661 aaaggtattt tgccagctgc cgtcgtcata ttggcgagct tgctgacgcg actgcgcagg
     9721 cattaaacga tcagcacaat ccatcgcccg cagccagtaa agcggattgg tttcggttga
     9781 tttaccttgc agcgcccaga tgtcgctaca ttcagtagaa agatagtcag ccagttgata
     9841 aaccggaatt ttttcttctg ctggcgtatc aatggctggc ttattgtgat tctgcacgca
     9901 acccagcaat gccagacatg gcagccctgc cagccacagc cgtcggggca ataatcgttg
     9961 aaaaatgtgt cgcatattca ccagacttaa agcctatccc agtgggcgta attgttgcag
    10021 acagtctgga catggacagc gcggagaaac cggggcgtac atatcgtacg tgaagatttc
    10081 gagcactacc cggggccgaa ctgacaaata aaatagcctg ataggatagg ctgttagcat
    10141 tatttcgtgt ttttcgacga cggtaattca atgcggaaac aaacgtcttg cccgctctcg
    10201 tcgaccagat acagttcccc ttgcatacgg cgaatacaat ccctggcaat gcttaatccc
    10261 agaccgctgc ccttcaccgc cccttttcgc tggtggcttc cctgaaaaaa gggttcgaag
    10321 atcatggcgc gttcctcttg cggaatgggc gtgcctgtat tgatgacatc aatataaacc
    10381 cgcgcaccat gtaaactgct gcgaaggcaa atgttaccgg attcagcccc gtagtgcacc
    10441 gcattggagt aaagattatc cagtacgctc atcagcagca ttggctccgc caagcaagct
    10501 gttgctttga gatcgacgtc ggtatgcatc attttagccc gtgcgggcag gctatgagca
    10561 gaaaccactg tctccaccag cggtgctaat tcaacattct ccagttccac cgcactgtcc
    10621 gcctgtttac ggttgtaatc aagcagttgt tcgatcagtt tttgcaaatt gcagctgctg
    10681 ctatcaagaa tgctcaccac ctctttttgc tctggcgtaa gcggcccgac aacctgatca
    10741 gccagtaatt cagtgccctc gcgcatactc gccagtggcg tttttaattc atgagataaa
    10801 tgtcttaaaa attgatggcg ttgggattcc agccatgaca ggcgctcact taaccaaaga
    10861 atacgttgcc caaccgagcg taactcgctc ggtccacaga acgaaacgct attgcccaga
    10921 gaacgccctt cccccagacg gttgatcatg cgctcgatat ttttcaccgg cccgataatc
    10981 atccgcgtga aaagtagcac catcaccaga ctcaccagaa atagcaccag cgattgccaa
    11041 ccaaaatatt gcccacgttc ggcgatttca cgctgaagtt gctgcccacg agagaacacc
    11101 actgtgcgcg tggcctgcac catttcggta ttggcactgg caaaggcttc aagacgcgcg
    11161 gcggcggcag catcgggacc actgttgtta cactgaagtt gggccagatt gttcaaatcc
    11221 tgacgcaatg cctggtaaag tttatcgtct ggcagtacgc ctgcgtgggc atcgagcatt
    11281 tcgctgtaac gcttgcgctg gctttgataa accttcgcaa gcgtagggtc gtccagcacg
    11341 caatactgac ggtagctacg ctccatctcc agcgccgcgt tggtcatcgc ttcactacgc
    11401 cgggcatcaa taagcgtagt gcggttaacc agcgccgcct gatcgctaag cgcattcagg
    11461 ctttgccagg cttgccatgc cagcaccaac aggggtagta gaattagcaa aaatgccagc
    11521 attaccagtt gtcgtaatga gcggggaaaa acgggccagc gtttcaaggt gttactctcg
    11581 tcagacgcga atagcctgat gctaaccgag gggaagttca gatacaacaa agccgggaat
    11641 tacccggctt tgttatggaa taaggcggtg cctaactcga cgtttcgccc gatggttgat
    11701 atagctacgc tgatatcaga agttggacgg caggcacctt gttgtgcgtc attcgaattt
    11761 tatgtagcac gtcccgaagg ggctgacata agtcggtgaa tgagccactg cttactatta
    11821 tgcagtaact gtgccaataa agaaaatagt ttggtaacgt actgattata cgttgctttg
    11881 agagatttat ggcttcttcg ctatttgaat gtaaatcagc cactgtgtcg ctaaaaagag
    11941 acaacttaag ataaacttat tagattacaa taaaatcaat catttaagtg tcgccagaaa
    12001 gcgacacggt aaaccaccca ttgtcgtgat ttacagacac aaaaaagctc ccagagttgg
    12061 gagcttatga tagtggttgg tgcttaatgc cgcatccggg ctgcaaaacc aatgaactga
    12121 tgacttaccc caactgctta cgcgcattgc ggaaaatgcg catccatggg ccatcctcgc
    12181 cccagttttc cggatgccag gagttgctga cagtacggaa aacacgttcc gggtgcggca
    12241 tcataatggt gactcgacca ctttcagtcg taaccgccgt aataccgttc ggtgaaccgt
    12301 tcgggttagc cgggtaggtt tcagtgactt tgccgaagtt atcgacatag cgcagtgcca
    12361 ccagcccttt gctttccagt gccgccagat gcgccgcatc acgcacttcc acgcgccctt
    12421 caccatgaga gacagcaatc ggcatttgcg agcccaccat cccctgcaac agcagagacg
    12481 ggctttgggt tacttcaacc aggctgaaac gcgcttcaaa gcgatcggag gtgttacgca
    12541 caaaacgtgg ccacaactcg ctccccggga tcagttcacg caggttagac atcatctggc
    12601 aaccgttaca tacccccagg gccagcgttt gcggacgatg gaagaaggtt gcaaactcat
    12661 cgcgtacacg gtcattgaac aggatagact tcgcccaacc ttcaccggca cccagcacat
    12721 caccgtagga gaaacctccg cacgcgacca gggcgtgaaa atcctccagt cccgtacgtc
    12781 cagtcagcag gtcgctcata tgcacgtcga tagcatcaaa gcccccacgt tggaaagctg
    12841 ccgccatttc aacatgcgag ttcacgccct gctcacgcag tacggcaact ttcggacgtg
    12901 cgccagtggc aatatacggt gctgccacat cttcgttgat atcgaacgac agttttacat
    12961 tcaggcccgg atcggcgtcg ttagatttcg cctgatgctc ctgatcggca cactccgggt
    13021 tgtcacgcag gcgctgcatc tgccaggtag tttctgccca ccagacacgc aacgtagtgc
    13081 ggctttcgct gaatacagtc tgcccgttgg cggtaatcac aaaacggtca ccggaaaccg
    13141 cctgcccaac ataatggacg caatccgcaa gcccatgctg tgccagtacg gactcgaccg
    13201 cttcacggtc agcggcacga acctgaatca ccgcacccag ttcttcgtta aacaacgccg
    13261 ccaggcgatc gtcacccaga gaggcgatat ccgcgtcaat gccacaatga cctgcaaagg
    13321 ccatttccgc cagcgttacc agcaggccgc catcagagcg gtcgtgatac gccagcagct
    13381 tacgctgtgc aaccagcgcc tgaatcgcgt catagaagcc tttcagttgg acaacatcgc
    13441 gtacatctgc cggtttgtcg ccaagctgac gataaacctg cgccagcgcc gttgcgccca
    13501 gcgcgttatt gcctttgccc aaatcaatca gcagcagtgc gttatcttcg gtacaaagct
    13561 gcggcgtgat ggtgtgacgt acatcttcca cgcgggcaaa tgcagaaatc accagcgaca
    13621 gcggcgacgt catttcgcgc tcttcgttac cttcctgcca gcgggttttc atcgacatag
    13681 agtctttacc caccgggatc gtcaggccca gcgccggaca aagttcttca cccacggctt
    13741 taacggcttc atacaggccc gcatcttcac cagggtggcc tgccgccgcc atccagttgg
    13801 cggaaagttt aatgcgtttg atatcgccaa tctgtgttgc ggcaatattg gttaacgctt
    13861 caccgaccgc cagacgggca gaggcggcga aatccagcag cgcaaccggc gcacgctcgc
    13921 caatcgccat cgcttcaccg tagtagctgt cgaggctggc ggtagtgacc gcgcagttag
    13981 cgaccggcac ctgccacggc cccaccatct gatcgcgcgc caccatgccg gttacgctgc
    14041 ggtcaccgat ggtcaccagg aaagttttct ccgccacggt cggcagatgc agtacgcgtt
    14101 tcaccgcgtc tgcaatggtg atcccttcac ggaccagcgc atcgcccttc gctttcagcg
    14161 tttgtacatc gcgggtcatc ttcggcgttt taccaagcag gacgtccagc ggcagatcga
    14221 tcggctgatt atcaaaatga cgatcgtgca gagaaagatg cagttcttcg gtcgcttcac
    14281 caatcaccgc gtagggtgcg cgctcacgct tacacagttc gtcaaacagc ggtaattgat
    14341 cggcagcaac cgccagcacg tagcgttcct gggattcgtt acaccagatt tccagcgggc
    14401 tcatgcccgg ttcgtcgctt agaatatcgc gcagttcaaa tttaccgccg cgtccgccgt
    14461 cgctcaccag ctccggcatc gcgttagaaa gaccgcccgc accgacgtcg tggataaaca
    14521 gaattgggtt agcatcacca agctgccagc aacggtcgat cacttcctgg caacgacgct
    14581 ccatctccgg gttgtcgcgc tgtacggaag caaagtcgag gtcggcatca gactgaccag
    14641 acgccatgga agacgccgcg ccgccgccaa gaccgatgtt cattgccggg ccgccgagaa
    14701 cgaccagctt cgcaccgacg ttgatctcgc ctttttgtac gtgatcggcg cgaatgttgc
    14761 cgatcccgcc cgccagcatg atcggtttgt gataaccgcg cagctcttcg ccattgtggc
    14821 tgttcacttt ttcttcataa gtacggaagt agccgttcag tgccggacga ccaaattcgt
    14881 tgttaaacgc cgcaccgccc agcgggcctt cggtcatgat gtccagcgcg gtaacaatgc
    14941 gctcaggctt accgaaatct tcttcccacg gctgttcgaa gccaggaatt cgcaggttgg
    15001 atacggagaa accaaccaaa ccagctttcg gcttcgcgcc gcgcccggtg gcaccttcat
    15061 cgcggatttc accgccggaa ccggtcgccg cgcccggcca cggagaaatc gccgtcgggt
    15121 ggttgtgagt ttcgactttc atcaggatgt gcgccggttc ctgatggaaa ttgtagcggc
    15181 ccgtttcgtg gtcagcaaag tagcggccca cttcagaacc ttccattacg gcggcgttat
    15241 ctttataagc agagagaacg tgatctggcg tggtttcgaa agtatttttg atcatcctga
    15301 acagcgattt cggctgttgt tcaccgtcga taatccagtc ggcgttaaaa attttgtggc
    15361 ggcagtgctc agagttcgcc tgagcgaaca tatacagctc gatgtcgttc gggttacgac
    15421 caagctttgt gaaagcatcc tgcagatagt caatttcatc ttccgccaga gccaggccaa
    15481 gacgcaggtt agcgtcgatc agcgcctgac ggccctgccc cagcaaatca acgcttgtga
    15541 ccggagtcgg ttgatggtgg gcaaacaact gctctgcatc atctaaatca aaaaagaccg
    15601 tttccatcat gcggtcgtgc agttcagcgg taacctgctg ccattgttca ttggtcagcg
    15661 taccggcttc tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt tgtagcccgc
    15721 agttatgggc aatatcggtc gctttcgaag accagggaga gatggtgcca ggacgcgggg
    15781 tcaccagcag gagtttgcct tgcggggcgt ggctggcgag tgccggacca tatttcagca
    15841 ggcgttcaag ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga
    15901 catactcggc gtaaatattg tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt
    15961 tgttgattcg gaatgccgac agtgcaggcg aaccacgcag aatttccatc ataagtctct
    16021 cgtcttctaa gctttcggtg tacccaaggg ggggaaacgg gcgtcattat aaagaatctg
    16081 atgcgctgac gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg
    16141 ccgccaatgg ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt
    16201 atgagtaaac ctttcactat tattacgttt tttcaagctg ggacgcgcac gacacagaga
    16261 attaactaat tgaaaaaatt aaagattaat tatctgttca tcggcattct ggcactgctg
    16321 ctcgcggtcg ctctctggcc atccattccc tggtttggta aagccgacaa ccgtatcgcc
    16381 gccattcaag cgcggggaga gttgcgtgtg agcaccattc atactcccct gacttataac
    16441 gaaatcaacg ggaaaccttt tggcctggat tacgaactgg cgaaacagtt tgccgattac
    16501 ctcggcgtaa aactgaaagt gaccgtgcgg cagaatatca gccagctgtt tgacgacctt
    16561 gataatggta acgccgacct gctggcggca ggactggtct ataacagtga gcgggtaaaa
    16621 aattatcagc ctggccctac ctattattcc gtgtcacaac aactggttta taaagtgggt
    16681 cagtatcgcc cacgtacgct gggcaacctg acggcggagc aactcaccgt tgcaccgggt
    16741 catgtggtgg ttaacgatct ccagaccctg aaagaaacaa aattcccgga attaagctgg
    16801 aaggtagacg acaaaaaagg ctctgcggaa ttaatggaag atgtcatcga aggaaaactc
    16861 gattacacca ttgctgattc tgtcgccatc agcctgtttc agcgcgttca cccggagctc
    16921 gccgtagcgc tcgatatcac cgatgaacaa ccggtgactt ggtttagccc gttagatggc
    16981 gataataccc tttccgccgc cctgctcgac ttcttcaacg aaatgaatga agacggtacg
    17041 ctggcacgca ttgaagagaa atacctgggg catggcgatg attttgatta cgtcgatacg
    17101 cgcacatttt tacgcgccgt cgatgcggta ctgccgcagt taaagcccct gtttgagaaa
    17161 tacgccgaag aaattgactg gcgtttgctg gccgctattg cttatcagga atcgcactgg
    17221 gatgcacagg ccacttcacc gacgggtgtg cgcggcatga tgatgttaac caaaaatacc
    17281 gcgcaaagcc tcggcattac ggatcgtacc gatgccgaac agagcatcag cggtggcgtg
    17341 cgttatttgc aggatatgat gagtaaagtg ccggaaagtg tgccggagaa cgaacggatc
    17401 tggtttgccc tcgctgcgta caatatgggc tatgcgcata tgctggatgc ccgcgccctg
    17461 acggcaaaaa ccaaagggaa tcctgacagt tgggctgacg taaaacagcg tctgccttta
    17521 cttagccaga aaccctatta cagcaagctg acttacggct acgctcgtgg gcatgaagcc
    17581 tacgcttatg tcgaaaatat tcgtaagtat cagattagcc tggtgggtta tctgcaagag
    17641 aaagagaagc aggctacaga agcggcgatg caactggcgc aggattatcc ggcggtatcg
    17701 cctacggagt tgggcaaaga gaaatttcct tttctctcgt ttctttccca gtcgtcatca
    17761 aactatttga cccattctcc ctctctgctg ttttccagga aagggagtga agagaaacaa
    17821 aattaatccg tcgaggattg cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga
    17881 aagaagtcac tgagcaacgc cgcgcactca tccgccagta ttccttccgt aatttccact
    17941 cggtgattca tacccggatg atgcagcaca tccattaaag atcccgcagc gccagttttc
    18001 gcgtcacgcg caccaaagac cacgcgacca atgcgactgt ggatcatcgc tccggcacac
    18061 attacacatg gttcaagcgt gacatacaac gtggcgtcga tcagacgata attttgcatc
    18121 accagaccac cctgccgcag ggccatgatt tctgcatgtg cggtgggatc atggcgacca
    18181 atcgggcggt tccagccttc gccgattacc cgattgttat gcactaatac cgcgccgacc
    18241 ggcacttccc gctcatccca ggcacgtttc gccagcgtca gcgcgtgacg catccagtat
    18301 tcgtggctaa attcgacttc agacaaaaag aaaactccgg ttataaaagc gcggcgcatt
    18361 atacacggac gctatgcttt actctattcc agttgctgga gttcaccgcg cggggttact
    18421 cgccaacgat gctgacagaa ataaagcagc ggattgtcct gattactgtc gctatagcca
    18481 ctgtacagcc gcagcggcgt gccgattttg cgctccagtt gtgcgacctt ttcatgtccc
    18541 agacaacgca tcgtcaatac ccaaccacca tagccacgct gaatttggct ggcgataaga
    18601 ttaacccgcg gcagccaggg cgtatcgaaa taaaccgctt caaccagcgg ctgcggagag
    18661 ccggtaatca accagatatc agcatcggaa cttaacaggt aggtggttaa tcgctcctga
    18721 accagcggaa aggcggtaac attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc
    18781 tgtaaacgtg cttcgctgtg accaaaagtg cacccccaca gaagcagact catcggccag
    18841 cgtgccgcac gaccttttat caataacgca atggctataa tcggtaacaa cggcaggaca
    18901 agtaacgcat tcagcggttg gcgacgcagt aaatagcgca gaaaactgcc gaacatatcc
    18961 tgctgatgca atgttccatc taagtcaaaa aacaccacac gacgctcgtg agttgccaaa
    19021 ccatactcct ctggactgaa acatcctgat taattttcgc tactacatag cctaacagat
    19081 agatcatcac ttttccggca acaatcgacc gcatcatggc taactggaat ttttaattca
    19141 tggcaattag cggcaatgga atataaaatt cactcgcgtg tgtctcatat tgcctgttgt
    19201 cgccactatt tcatcgcgag gaaagatatg aacggcttac ttcgtatccg tcagcgttac
    19261 caggggcttg cccagagtga taaaaaactg gcggattatc tgctgctaca acctgatacg
    19321 gcgcgccatt taagctctca gcaactggcc aacgaagccg gagtcagtca gtccagcgtc
    19381 gtgaagttcg cgcaaaaact cggctataaa ggttttccgg cgcttaagtt ggcgttgagt
    19441 gaagcgctgg caagccagcc ggaatcgccc tccgtgccca ttcataacca aatccgcggt
    19501 gatgatccgt tacggctggt cggcgaaaaa ctgattaaag aaaatactgc cgctatgtac
    19561 gcaacgctaa acgttaatag tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct
    19621 gcgcggcgga taattctgac cggtattggc gcttcgggtc tggtggcgca aaactttgcc
    19681 tggaagctga tgaagattgg cttcaatgct gccgcactgc gcgatatgca tgcgctactc
    19741 gcaacagtac aggcgtcgtc ccctgacgat ctgttattag ccatttccta caccggtgta
    19801 cgacgcgagt taaacctggc ggcagatgag atgctgcgag tgggcggaaa agtgctggcg
    19861 attaccggct ttactccgaa tgccctgcaa cagcgtgctt ctcattgcct gtataccatt
    19921 gccgaagaac aagcgacaaa cagtgcttca atctctgctt gtcacgctca gggaatgtta
    19981 acggatttgc tgttcattgc gctgattcag caggatctgg aactggcacc agaacgtatt
    20041 cgtcatagtg aagcgctggt gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc
    20101 gcccggtttg tgttgttttg agagtttcct tatggcgctg ttaatcacta aaaaatgcat
    20161 caattgtgat atgtgtgaaa tcaaataaaa acacatttat tttcatagtg atacagcaat
    20221 gtgttattga atgtgttgtt ggatttatgg tgcaaaaaaa ttttgcataa gttcagcaac
    20281 acaaagaaca ccactttctt taacgtcctc cgcgccctaa aggacggggc tttacggcgc
    20341 aaaaggcctg tgacatacaa cattaacttt tcagtcaacc gtagcacgtc cttgtatacg
    20401 ccgtgtctgc ggttttatat aaccctggct ggaaacctct tatacaaagt cgattcgcca
    20461 acatcataga tgatcgccac cttctggcga gaaactcctg atgcaattag tcgcccggcc
    20521 tgcgcccatt attctggtga aagtttggga cggcgaccgc caattcgtcc ctgtgcgcga
    20581 gcagcttcca gtcctgcttt tgttcgttca acaatcagtt cacgttccat ttcagccagg
    20641 gcacccatca catggaagaa aaagcgtccc attggtgtgc tggtatcaat tgaatcagtc
    20701 agactacgaa agttgatgcc tcgttcgcgc aactcctcca ccagcacgac aagatgccgc
    20761 atactgcgcc ccagccgatc cagcttccag acaaccagtg tgtcgcctgc cgataatgtc
    20821 ctgagcagtt ttttcagccc cggtctttcg gactttgtac cacttatctt gtcttcaaaa
    20881 atcagctcgc atcctgcaca gttcaacgca ttacgttgta gatcggtatt ctggtcattt
    20941 gttgatacgc gtacatagcc aataagcatg gtaaatcccc
//
LOCUS       DAJVQH010000067        20548 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJVQH010000067 DAJVQH010000000
VERSION     DAJVQH010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.067"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OQM87_002457"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OQM87_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCS1508557.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OQM87_002458"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OQM87_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCS1508558.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OQM87_002459"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OQM87_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCS1508559.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OQM87_002460"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OQM87_002460"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCS1508560.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFCKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OQM87_002461"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OQM87_002461"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCS1508561.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4448..4813
                     /locus_tag="OQM87_002462"
     CDS             4448..4813
                     /locus_tag="OQM87_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCS1508562.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4963..5481
                     /locus_tag="OQM87_002463"
     CDS             4963..5481
                     /locus_tag="OQM87_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCS1508563.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5471..6696
                     /gene="dgcN"
                     /locus_tag="OQM87_002464"
                     /pseudo
     CDS             5471..6696
                     /gene="dgcN"
                     /locus_tag="OQM87_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6712..7194
                     /locus_tag="OQM87_002465"
     CDS             6712..7194
                     /locus_tag="OQM87_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCS1508564.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQLQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7271..7618)
                     /gene="rplS"
                     /locus_tag="OQM87_002466"
     CDS             complement(7271..7618)
                     /gene="rplS"
                     /locus_tag="OQM87_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCS1508565.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7660..8427)
                     /gene="trmD"
                     /locus_tag="OQM87_002467"
     CDS             complement(7660..8427)
                     /gene="trmD"
                     /locus_tag="OQM87_002467"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCS1508566.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="OQM87_002468"
     CDS             complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="OQM87_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCS1508567.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="OQM87_002469"
     CDS             complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="OQM87_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCS1508568.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="OQM87_002470"
     CDS             complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="OQM87_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCS1508569.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10938..11729
                     /locus_tag="OQM87_002471"
     CDS             10938..11729
                     /locus_tag="OQM87_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCS1508570.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11750..13036
                     /locus_tag="OQM87_002472"
     CDS             11750..13036
                     /locus_tag="OQM87_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1508571.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="OQM87_002473"
     CDS             complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="OQM87_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCS1508572.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13807..14685
                     /gene="nadK"
                     /locus_tag="OQM87_002474"
     CDS             13807..14685
                     /gene="nadK"
                     /locus_tag="OQM87_002474"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCS1508573.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14771..16432
                     /gene="recN"
                     /locus_tag="OQM87_002475"
     CDS             14771..16432
                     /gene="recN"
                     /locus_tag="OQM87_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCS1508574.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16581..16922
                     /gene="bamE"
                     /locus_tag="OQM87_002476"
     CDS             16581..16922
                     /gene="bamE"
                     /locus_tag="OQM87_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCS1508575.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16984..17274)
                     /locus_tag="OQM87_002477"
     CDS             complement(16984..17274)
                     /locus_tag="OQM87_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCS1508576.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17264..17701)
                     /gene="ratA"
                     /locus_tag="OQM87_002478"
     CDS             complement(17264..17701)
                     /gene="ratA"
                     /locus_tag="OQM87_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCS1508577.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17872..18354
                     /gene="smpB"
                     /locus_tag="OQM87_002479"
     CDS             17872..18354
                     /gene="smpB"
                     /locus_tag="OQM87_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCS1508578.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18569..18931
                     /gene="ssrA"
                     /locus_tag="OQM87_002480"
     tmRNA           18569..18931
                     /gene="ssrA"
                     /locus_tag="OQM87_002480"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19136..20377
                     /locus_tag="OQM87_002481"
     CDS             19136..20377
                     /locus_tag="OQM87_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1508579.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDDDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRQCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5305 a         5368 c         4822 g         5053 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac catcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcgct ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcag aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccaa gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc agtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcattaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat atataaattt acaccccttt gaaaacaatc cgctatgctt tgaaaaagga
     4441 gaaagaaatg atgaaaaagt ttatcgcccc cttgttggct ttactggtta gcggatgtca
     4501 gattgatcct tatactcacg cgccaacctt gaccagcacc gactggtatg atgtcggtat
     4561 ggaagatgcg atatcggaca gcgccataaa agatgacgat gcatttagcg attcacaggc
     4621 ggatcgcggt ctatacctta aaggatatgc cgaagggcaa aagaaaactt gccagaccga
     4681 ttttacttat gcccggggac tttccggtaa aagctttcct gccagctgca acaatgttga
     4741 aaatgccagc caactgcatg aagtatggca aaaaggggct gatgaaaatg ccagcacaat
     4801 acgattaaat taattaaaac cccacaaata aattaaacat aagattttac tcattgtgtt
     4861 ttatttctca cattgatgac ggtcgcatga aaaaatgata ttttaatgag catcctgaaa
     4921 aaatatcgat gcatttcgag cgaagatggt ggggatccct gaatgcgttt ttctcaccga
     4981 ctgttcttac ttctgattct cctgctgacg ggagcaccaa tactcgctca agagccttct
     5041 gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac ccgctggcct
     5101 gcgctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag taccgcccta
     5161 caagagaatg ccgcgacatc tttgccttat ctgcctgtca tcattcacac tcaacaagag
     5221 gcgatgattt caggctgcaa tggtttttat tttggcaacg agtcaccgac attccagatg
     5281 gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca aaataccgag
     5341 tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag atttgccgta
     5401 aacctggatg ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt aatgctcgca
     5461 cggaagaaaa atgatggata acgataattc tcttaataag cgccccacgt ttaaaagagc
     5521 attacgcaac atcagtatga ccagcatatt tatcactatg atgttgatct ggttgctgct
     5581 ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa aaaaacctgg cactgacagc
     5641 agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc ctgcagcaac
     5701 tgaaacactg gcagcgctgg gccagcaagg gcattttcaa ctgcagaagt acgtgataag
     5761 cagcaaaata ttctggcatc ctggcattac ccccgtaagg atccaggcga tactttcagc
     5821 aatttcataa gccactggct cttccctgcc cccatcattc agccgattcg tcacaatggt
     5881 gaaaccattg gcgaagtacg cttaaccgct cgcgacagtt caattagcca ttttatctgg
     5941 ttttcactcg ccgtactgac cggttgtatt ctgctggcat caggcatcgc aattaccctc
     6001 acccgccatt tgcacaatgg cctggtggaa gcactgaaaa atatcaccga tgtcgtacat
     6061 gatgtgcgtt ccaaccgcaa tttttcccga cgagtttcgg aagaacgtat cgctgagttt
     6121 caccgcttcg ctctcgactt caacagtctg ctggatgaaa tggaagagtg gcagcttcgt
     6181 ttacaggcca aaaatgcgca gcttctgcgt accgcgctac atgacccatt aaccgggctg
     6241 gctaaccgcg cagcgtttcg tagcggcatc aatacgttga tgaacaattc cgatgcccga
     6301 aaaacgtcgg cgttactatt tcttgatggc gataatttca aatatatcaa tgatacctgg
     6361 ggtcatgcga cgggcgatag ggtcttgatt gaaatcgcaa aacggttagc tgaatttggc
     6421 gggctgcgac ataaagcata ccgcctgggc ggcgatgaat tcgctatggt gctctatgat
     6481 gtacagtcgg aatctgaagt gcagcagata tgctcagcac tgacacaaat ctttaatctc
     6541 ccgtttgatc ttcataatgg ccatcagacc accatgacat taagcattgg ttacgcgatg
     6601 accattgagc acgcctctgc ggaaaaatta caagagcttg ccgatcacaa tatgtatcag
     6661 gccaaacacc agcgtgccga aaagctggtg agataacaag gatatatcga tatgataaag
     6721 cacctggtag cacccctgat tttcacctca ctaatactga ctggctgcca gtcccctcag
     6781 ggaaagttta ctcctgagca agtcgccgct atgcaatctt atggatttac tgaatccgcc
     6841 ggcgactggt cgctgggctt atcagatgcc attctgttcg caaaaaatga ctacaaattg
     6901 ctcccggaaa gccagcaaca gctccaaacc atggcagcca aattggcctc gacagggcta
     6961 acacatgccc gtatggatgg acacaccgat aactatggtg aagacagtta caacgaaggc
     7021 ttatcattga aacgggcgaa tgtcgtggcc gatgcatggg ctattggtgg acaaattcca
     7081 cgcagcaatc tcaccacaca gggtttagga aaaaaatatc ccatagccag taacaagacc
     7141 gcccagggcc gcgccgagaa ccgccgcgtc gcagtggtga ttacaacccc ttaaaacacg
     7201 agcaaacaga caaaaaaagg gccagccaat tgcccagccc ttcttaacag gatgtcgctt
     7261 aagcgaaatc ttagttaaga cgctctttga tacgagcagc cttaccagta cgctcacgca
     7321 ggtagtacag tttagcttta cgaacagcac cacgacgttt gacagaaatg ctgtcaacta
     7381 ccggagagtg agtctggaag acacgctcaa cgccttcgcc gttggaaatt ttacgaacag
     7441 tgaatgcaga gtgcagaccg cggttacgaa tagcgataac cacgccctcg aatgcctgca
     7501 gacgtttttt ggaaccttca acaacccata ctttcacttc cacggtatca cccggacgga
     7561 aggaaggtac gtcctgcttc atctgctctt gttcaagttg cttaataatg ttgctcataa
     7621 tttaatctct tatcctgggt aaactgatct ctcgggggct tacgccatcc catcatgttt
     7681 atgttgctgt tgtgcgtgtt ccgttttgaa ctccgccagc aaccttgctt gctcttcagt
     7741 cagagccagg ttttccagaa gttcaggtct tctaagccag gtacggccca gcgactgttt
     7801 caaacgccag cgacgtatct cggcatggtt gcccgacagt aacactggcg gaacttccat
     7861 cccttctaac acctcaggcc gcgtatagtg cgggcaatcc agcaatcctt cagcaaagga
     7921 atcttccgtt gccgaggctt catgtcccag tactcccgga ataaaccggg aaacggagtc
     7981 aatcagcgtc attgctggta actcaccacc actgagaacg taatcgccga ttgaccattc
     8041 ttcgtcaatt tcggtttgga tcacgcgctc atctatacct tcgtagcgac cgcacaccag
     8101 aatcaatttt tgattcgttg ccagttcgct gacgcccgct tgatcaagct tgcgtccctg
     8161 tggtgacaga taaatcacct ttgcgccttc acccgccgcg gcttttgctg catgaatggc
     8221 gtcccgcaag ggttgcacca tcattaacat ccccggtccg ccgccgtaag gacgatcgtc
     8281 cacggtacgg tgccggtcat gcgtgaagtc gcgaggactc caactctgga tgctcagcag
     8341 gccattttta actgcccggc cagttacccc gtaatcggta attgcgcgga acatttcagg
     8401 aaacaggcta attatgccaa tccacatagc gccgtctttt accgtttatc cggtggttta
     8461 aaaaccagga tcccaatcta cttcgattga acgagtagtg agatcgactt tcttgataac
     8521 ctgcccatcg aggaacggta cgagacgttc cttgatacca aacgcatctt tcaggtttgc
     8581 cttaatgacg agaacgtcat tagatccggt ttccatcata tcgacgactt taccgagatc
     8641 gtagccttca gtggttacta cctggcagcc catcaggtct ttccagtagt agtccccctc
     8701 ttcaagctga ggcagctgcg atgaatccac gacaatttca caattcgtca gcaggttcgc
     8761 cgcatcacga tcgtcaacgc ctttcagctt gatgatcatg tcctgattgt ggtgcttcca
     8821 gctttccagc tggacttgct gccactgacc cgccttctgg ataaaccagg gctgatagtc
     8881 aaaaatgctt tcggcgtctt cggtggaaga aaacactctg agccacccac gaataccgta
     8941 agacgaaccc atttttccca aaacgatggg atcaacaggt gcttgcgcgg tgagttgttt
     9001 gctcatcatg accaccgtga cagattaagc tgctttgttt acttctttga tcagcgcagc
     9061 aacgcgatca gaaatagttg cgccctggcc aacccagtga gcgatgcgat ccagatccag
     9121 gcgagtgcct tcttcttttt cgctagcgat tgggttgaag aaaccaacgc gctcgatgaa
     9181 gcgaccgttg cgtgcattac ggctgtcagc gacaacaacc tggtagaacg gacgcttttt
     9241 agcgccgtga cgtgctaaac gaatagttac cataacatcc tcttgtgtga ataaaacaac
     9301 cggaccccat cgaggaacgg agtccggtgt catattaaaa gcccgaaaat tttactgatt
     9361 tctggagaaa atgcaatcag cagttaataa ctctgtcgca taaggtcatt tagcgaccag
     9421 gaaatcccgg cggcatcatt cccttcatgc ttctcatcat cttcgccatt ccgcctttct
     9481 tcattttctt catcatgcgc tgcatgtcgt cgaactgctt cagaaggcgg ttaacgtcct
     9541 gtacctgcat cccgcaaccg gcggcgatac gacgcttacg cgaacctttg atgatttctg
     9601 gcttagcgcg ctctttcatc gtcattgagt tgatgatggc ttccatacgc accagcactt
     9661 tatcgtccat ctgcgacttg acgttatccg ggatctgccc catgcccggc agtttgccca
     9721 tcaagctggc catgccgccc atatttttca tctggcgcag ttgctcaaga aagtcgttga
     9781 gatcgaagcc gtcgcctttt tttagcttgc tggctaattt ctctgcctgc gcgcggtcaa
     9841 ctttgctttc gatatcttcg atcagcgaca gtacgtcgcc catgccgaga atacgcgacg
     9901 cgatgcggtc cggatggaac ggctccagcg cctcagtttt ctcgccaaca ccgaggaact
     9961 tgatcggttt gccagtgatg tgacgaatag agagcgccgc accgccgcgg gcatcgccgt
    10021 ccactttggt caacactacg ccggtaagcg gtaacgcttc attgaatgct tttgccgtat
    10081 tggccgcatc ctgaccggtc atggcgtcaa ccacaaacag ggtttcaacc gggttaatcg
    10141 acgcatggac ttgtttgatc tcgtccatca tcgcttcgtc aacgtgcaga cgaccagcgg
    10201 tatccaccag cagcacgtcg tagaatttca gtttggcttc tttcagcgcc gcgttaacaa
    10261 tatcaaccgg cttctgacca acatcagaag ggaagaaatc aacgcccacc tgctctgcca
    10321 gcgtctcaag ctgtttgatt gccgccgggc gatagacgtc agcagaaacc accagcactt
    10381 tcttcttgtg cttctcgcgc aggaaattac cgagtttacc aacgctggtt gttttaccgg
    10441 caccttgcag gcctgccatc agcacgaccg caggcggttg cgcagccaga ttcagggtct
    10501 ggttctcttc gcccatcgcc gcaaccagtt cgttacggac gattttgacg aactcctgcc
    10561 ccggcgtcag gctcttatta acttcatgac caaccgcttt ctcttttacg cgattgataa
    10621 actcacgcac taccggcaga gcgacgtccg cctccagcag cgccatgcgc acttcgcgca
    10681 gcgtatcttt tacgttgtct tcagtgaggc gtccacggcc actgatattg cgcagcgtgc
    10741 gcgacaaacg atcggttaaa ttatcaaaca ttgtctctcg cctggggtgg aaacggttgg
    10801 ccgcaatcgc gacacatcat cagtattttg ccgcagtata acatgaaggc gtctttgttg
    10861 ttatgcaacg gttggagcag cgttcacctg acgctatact gcttctcttt cttattgctc
    10921 aaactgtcga cattactatg cccgtttttg ctctgctcgc gcttgtcgcc tactccgtca
    10981 gtcttgcgct gattgttccc ggtctgctgc aaaaaaacgg cggctggcgg cgcatggcta
    11041 ttatttctgc ggtcattgcg ctggtctgcc acgcaatcgc tctggaagcc cgcatcctgc
    11101 ccgacggtga tagcggacaa aacctcagcc tgctgaacgt tggttcattg gtcagtttgg
    11161 tgatctgtac ggtaatgacc attgtggctt ctcgcaatcg tggctggttg ctgctaccca
    11221 ttgtctatgc ctttgcgctt atcaacctgg cgctggcgac cttcatgccc aatgaataca
    11281 tcacccatct ggaagcaacg cctgggatgc tggtgcacat tggcttatcg ctcttttcct
    11341 atgccacgct aattatcgcc gccctgtacg cgctgcaact ggcgtggatt gattaccaac
    11401 tgaagaacaa gaagctggca tttaaccagg aaatgccgcc attgatgagt atcgagcgta
    11461 aaatgttcca catcacgcag attggcgtgg tgctgctaac gctcacgctt tgcactggcc
    11521 tgttctacat gcacaaccta tttagcatgg aaaatatcga caaggctgtg ctctctatcg
    11581 tggcgtggtt tgtctatatt gtgctgctgt ggggacatta ccatgaagga tggcgtggac
    11641 gccgcgtcgt ctggtttaac gttgcgggcg cggtaattct gacactggcc tacttcggca
    11701 gccgaattgt ccagcagtta atcagctaaa cccagaaaag gagtttcccc tggaacacat
    11761 ttctactact acgttgatca ttattctgat catcatggtg gtcatttcag cctatttttc
    11821 cgggtccgaa accggaatga tgaccctcaa ccgctatcgt cttcgacata tggcgaaaca
    11881 gggtaatcgc tcggccaaac gcgtcgaaaa attgctgcgt aagccagacc gcctgataag
    11941 cctggtgtta atcggcaata acctggtcaa tattcttgcc tccgcgctcg gcactattgt
    12001 cgggatgcgt ttgtacggcg atgcgggcgt ggcaattgcg actggtgtgc tgacttttgt
    12061 cgtactggtt tttgctgagg tattgccgaa aaccattgcc gcgctgtacc cggaaaaagt
    12121 cgcttatccg agtagttttc tgctggctcc gctgcaaatt ttgatgatgc cgctggtctg
    12181 gttgctgaat gctatcaccc gtatgctgat gcgcatgatg ggtatcaaaa ccgatatcgt
    12241 ggttagcggc tctttgagca aagaagagtt gcgcactatc gtgcatgaat cgcgctcaca
    12301 aatttcccgt cgcaatcagg atatgctgct gtcggtgctc gatctggaaa aaatgaccgt
    12361 tgatgacatc atggtgccgc gcagtgaaat tatcggtatt gatatcaacg atgactggaa
    12421 atctattctg cgccaactct cccactcacc tcacgggcgc atcgtgcttt accgtgattc
    12481 gctggacgac gccatcagta tgctgcgagt acgtgaagcc tggcggttga tgtcggagaa
    12541 aaaagagttc accaaagaaa ccatgctgcg cgccgcggac gagatctatt ttgtgcctga
    12601 aggtacgccg ctcagcacgc agttggtaaa gtttcagcgc aacaaaaaga aagtcggcct
    12661 ggtcgtcaac gagtatggag acattcaggg gctggtgacg gttgaagata ttctggaaga
    12721 gattgtcggc gatttcacta cgtcgatgtc gccaacactt gccgaagagg tcacgccgca
    12781 aaacgacggt tcggtgatta tcgatggcac cgccaacgtg cgggaaatca acaaagcctt
    12841 taactggcat ctaccggaag atgatgcccg cacggttaat ggcgtcattc ttgaggcact
    12901 ggaagagatc cctgtcgcag gcacccgcgt gcgtattggc gagtacgata tcgatattct
    12961 cgacgtacag gacaatatga ttaagcaggt aaaagttttt cctgtgaaac cgctgcgcga
    13021 gagtgtggcg gagtaacgaa aacggccctg cattcgcatg cagggccgtg aattattacg
    13081 aaaacagaaa ttacgctttt gctttcgcta cagttaccat cgccgcacga atcgtacgac
    13141 cattcagcgt ataacccttc tgcataatgc ccagtacgtt acctggcgca acgtcatcag
    13201 attccaccat tgcgatagcc tgatgcacat tcgggtccag cggcacgtta gtttcggcga
    13261 tcacttcaac accaaactta cgcacaacat ccagcatcga cttcagcgtc agctcaatgc
    13321 cttcaaccat cgcagacata tccgggttag ctttatcagc cacttccagc gcacgatcca
    13381 ggctatcaat caccggcagc aattcgttga tgaatttctc cagcgcgaat ttgtgggctt
    13441 tttcaatatc cagttcagta cgacgacgca ggttttccat ttcggctttt acgcgcaaaa
    13501 taccgtcacg ttcacgggtc tgggcttcag ccagctgagc ttcgagattc gcaatttttt
    13561 catcgcgcgg atccacctgc tcagcagaag cttctggctc aactgcctca atctcttcgt
    13621 gctgatccat gataatttct tccggggctt gcccctcagg cgttttctgt tctttactac
    13681 tcatgaattt ctccgcgttt ttttcgcatt catctcgcta acttcgctta ttatggggat
    13741 cagtttcagg gtttcaaggg aagcactcac attgtcatca atcttcgcaa caaggacctc
    13801 ggaaaaatga ataatcattt caagtgcatt ggcattgtgg gacacccacg gcaccctact
    13861 gcactgacaa cacatgaaat gctctaccgc tggctgtgca caaaaggtta cgaggtcatc
    13921 gttgagcaac aaatcgctca cgaactgcaa ctgaagaatg tgaaaactgg cacgctcgcg
    13981 gagattgggc aactggctga tctcgcggta gtcgttggtg gcgacggtaa tatgctgggc
    14041 gcggcgcgca cgctcgcccg ttacgatatt aaagttattg gaatcaaccg tggcaacctg
    14101 ggtttcctga ctgaccttga ccccgataac gcccagcaac aattagccga tgtgctggaa
    14161 ggtcactaca tcagcgagaa acgttttttg ctggaagcgc aagtctgtca gcaagattgc
    14221 caaaaacgca tcagcaccgc gattaacgaa gtggtacttc atcctggcaa agtggcgcat
    14281 atgatagagt tcgaagtgta tatcgacgag atctttgcgt tttctcagcg atctgatggg
    14341 ctaattattt cgacgccaac aggctccacc gcctattccc tctctgcggg cggtcctatt
    14401 ctgaccccct ctctggatgc gattaccctg gtgcccatgt tcccgcatac gttgtcagca
    14461 cgaccactgg tcataaacag cagcagcacg atccgtctgc gtttttcgca tcgccgtaac
    14521 gacctggaaa tcagttgcga cagccagata gcactgccga ttcaggaagg tgaagatgtc
    14581 ctgattcgtc gctgtgatta ccatctgaat ctgattcatc cgaaagatta cagttatttc
    14641 aatacattaa gcaccaagct cggctggtca aaaaaattat tctaatttta cgccagcctc
    14701 tttactgtat ataaaaccag tttatactgt acacaataac agtaatggtt tttcatacag
    14761 gaaaacgact atgttggcac aactgaccat cagcaacttt gctatcgttc gtgagcttga
    14821 gattgatttt catagcggca tgaccgtaat aactggcgag accggtgcgg gtaaatctat
    14881 tgcaatagat gccctcggtc tttgtctcgg tggtcgcgct gaagccgaca tggtgcgtac
    14941 cggcgctgct cgcgctgacc tgtgcgcccg tttttctctg aaagatacgc cagcggccct
    15001 gcgctggctg gaagaaaacc agcttgaaga cgggcatgaa tgtttgcttc gtcgcgtgat
    15061 cagcagtgat ggtcgctccc gtggtttcat caacggtaca gctgttcctc tgtcacaact
    15121 gcgcgaactg ggtcagttgc tgattcagat ccatggtcag cacgctcatc aattactcac
    15181 caaacctgag caccaaaaat tcctgcttga tggctatgcc aatgaaacct ctctacttca
    15241 ggaaatgacc gcacgttatc agttgtggca tcaaagctgc cgtgacctcg cgcatcatca
    15301 acagttaagt caggaacgcg ccgcccgtgc ggaactgctg caataccaat taaaagaact
    15361 taacgaattt aatccgcagc ccggagagtt tgagcaaatc gacgaagagt acaaacgtct
    15421 ggcgaacagc ggtcaattgc tgaccaccag ccagaatgca ttggcattaa tggccgacgg
    15481 tgaagacgca aacctgcaaa gtcagcttta cacggctaaa caactggtga gcgaattgat
    15541 tggcatggac agcaaactgt ccggcgtact tgatatgctg gaagaagcta ccatccagat
    15601 tgctgaagcc agcgacgaac tgcgccacta ctgcgatcgt ctggatctcg atcccaaccg
    15661 actgtttgaa cttgaacagc gcatctcaaa acagatttcg ctggcacgta aacatcacgt
    15721 cagccctgag gcattaccac agtattacca gtcgctactg gaagaacagc agcaactgga
    15781 cgatcaggcc gactcacaag aaacgctcgc gctggcggta acgaaacatc atcagcaggc
    15841 actggaaacc gcgcgcgcat tacaccaaca acgccagcaa tatgcagaag aacttgcaca
    15901 gctgatcacc gacagtatgc atgcgctctc aatgccgcat gggcagttta cgattgatgt
    15961 caaatatgac gagcatcacc tgggcgctga cggtgccgat cgtattgagt ttcgggtaac
    16021 caccaaccca ggtcagccaa tgcagcctat tgccaaagtc gcatccggtg gtgaattgtc
    16081 ccgcatcgca ctggcaatcc aggtcatcac ggcgcgtaaa atggaaaccc cggcactgat
    16141 ttttgatgaa gtggatgtag ggattagcgg tccaacagcg gcagttgtcg gcaaactgct
    16201 gcgtcaactc ggcgaatcaa ctcaggtgat gtgtgttacc cacctgccac aagtcgcggg
    16261 atgtggtcat caacactatt ttgtcagcaa agaaaccgat ggtgcgatga cagaaacaca
    16321 tatgcaatct ctggataaaa aagcgcggtt acaagagctg gcgcgcctgc ttggtggcag
    16381 tgaagtcaca cgtaacacac tggcgaatgc gaaagaactg cttgcagcgt aaactttttt
    16441 cctgcttcac ggtcagagta aacagcaaaa cgccgtaaga ccggaaagca aaaggtttta
    16501 aagtgatgaa aggtctatta tcatcggcat attacagatg agccacgtac tgctcgggcc
    16561 cgaaaaggaa tcaaatcact atgcgctgta aaacgctgac tgctgcagca gcagtactat
    16621 tgatgttgac cgcaggctgt tccactctgg agcgagtggt ttaccgtcct gacatcaacc
    16681 aggggaacta tctgaccgct aacgacgtat ccaaaatacg tgttggcatg acgcaacaac
    16741 aagttgcgta cgcattgggt acaccgctga tgtccgatcc atttggtacg aatacctggt
    16801 tctatgtctt ccgccagcaa ccaggtcatg aaggtgtaac gcagcaaacg ctgacgctga
    16861 cctttaacag tagcggtgtg ttgaccaata ttgataacaa acctgcgctg agtggtaact
    16921 aataataaag ttgctctcaa agacgttaaa aaaggtgctc aatgagcacc ttttttctgt
    16981 ctgttattta ttcgctgatt tttctgctcg ttgcctgcga agctctttcg gatcggcaat
    17041 gagagggcga taaatctcca cccgatcgcc atcatgcaca ctatcgctta gttttgccgg
    17101 acggctgtaa atgccgactt tgtttttagt taaatcgata tcggtacgca attccagcaa
    17161 gccactggcg cgaatagctt cttcaaccgt cgcgccctcc tgcagcgtca ctcgctgcag
    17221 gtactgcttc tcaggtagcg cataagccac ctcaacggca attttaccta gcactgtaaa
    17281 cctctttcgc acgaaccgta aaagcctgga ccatattagc cgccagctct ttaaacacgc
    17341 gaccaaaggc gagttcaatc aacttattgg taaactcaaa gtcgagatga aactcgatac
    17401 gacacgcctc ctggctcagc ggcgtaaact tccatccacc aatcaatttc ttgaacggcc
    17461 catccaccag attcataaga atactttggt tactggtcaa ctggttgcgg gtagtaaacg
    17521 ttttgctgat cccagcctta gagacatcta ccgccgcagt catctgccca ggagtggact
    17581 ccagaatccg acttccggta caacctggca aaaactgagg ataagactga acgtcattca
    17641 ctaactgata catttgctcc gcgctgtagg gtaccagtgc ggtccggcta atctgaggca
    17701 taacaatttc catcaacaaa aatccaacaa ataatatcat ttatcctgct gtaaaaaaaa
    17761 cgctatcccg gcgctgggta acatcgggtt catgctaaga tagagccttg tcccccgcag
    17821 gattgatatg gggtgttttc gatttcagat taccgatgat tcacgacgct tatgacgaag
    17881 aaaaaagcac ataaacctgg ttcagcgacc atcgcgctta acaagcgcgc ccgtcacgaa
    17941 tactttatcg aagaagagtt cgaagcggga cttgccctgc aaggctggga agttaaatcc
    18001 ctgcgcgcag gaaaagccaa tatcagcgac agctacgtcc ttctgcgtga cggagaggca
    18061 tttctgtttg gcgctaacat cacgccaatg gccgtggcct ccacgcatgt ggtgtgcgat
    18121 cctacccgta cccgcaagtt acttctcaac cagcgcgaac tggactcatt gtacggtcgc
    18181 gtcaatcgag aaggctatac cgtagtggcg ctctccctgt actggaaaaa tgcctggtgc
    18241 aaagtgaaaa tcggcgtcgc caaaggtaaa aaacagcacg ataaacgttc agatatcaaa
    18301 gaacgcgaat ggcaggtgga taaagcacgc atcatgaaaa acgcccaccg ttaaacctgc
    18361 actccaatta ttgaccagtt cctcaccgcg cctccctctc cggcggcgcg aatgaacatc
    18421 ttattggcta tcacatccga cacaaatgtt gccatcccat tgcttaatcg aataaaaatc
    18481 aggctacatg ggtgctaaat ctttaacgat aacgccattg aggctggtca tggcgctcat
    18541 aaatctggta tacttacctt tacacattgg ggctgattct ggattcgacg ggatttgcga
    18601 aacccaaggt gcatgccgag gggcggttgg cctcgtaaaa agccgcaaaa aatagtcgca
    18661 aacgacgaaa actacgcttt agcagcttaa taacctgctt agagccctct ctccctagcc
    18721 tccgctctta ggacggggat caagagaggt caaacccaaa agagatcgcg tggaagccct
    18781 gcctggggtt gaagcgttaa aacttaatca ggctagtttg ttagtggcgt gtccgtccgc
    18841 agctggcaag cgaatgtaaa gactgactaa gcatgtagta ccgaggatgt aggaatttcg
    18901 gacgcgggtt caactcccgc cagctccacc aaaattctcc atcggtgatt accagagtca
    18961 tccgatgaag tcctaagagc ccgcacggcg caagccctgc gggctttttt gtgccccgaa
    19021 tttgtcccgc gaagtctgat gccaactaat taaatccgat ccttttaggc accttgttag
    19081 gcacttcata aagctatatt gtctttgagg tgcctaaaac tatggaaacc cggcaatggc
    19141 aagacaaacc aaacctctat ccgttaagga aatcgaatct gctaaaccca aggaagcgga
    19201 ctacgttctc tatgatgacg atggccttga gctactcatt aaatccagcg ggagtaaaat
    19261 ctggcagttt cgctacattc gccctgtcac caagacacga gcaaagaaaa gcataggccc
    19321 ttacccgtca gttacccttg ccgatgccag aaactaccgt gcagagtccc gctcactcct
    19381 agctaaacag attgagccgc aggaacatca gcaagaacaa ttgcgtagtt cgctggaagc
    19441 caaaaccaat actttccagc tcgtagctga acgttggtgg aatgtgaaga aagccagtgt
    19501 gacagaggac tatgcggaag atatctggcg ctctcttgaa agagatgtct ttcctgcgat
    19561 tggcgacgtt agcgttacag atattaaagc tcatacactg gttcaggccg tacaaccggt
    19621 tcaggccaga ggagcactgg aaacggttcg tcgccagtgt caacgtatta atgaggtcat
    19681 gatctatgcc cagaacacag ggctgattga tgctgttccc agcgttaata tcggtaaagc
    19741 cttcgaaaag cctcagaaaa agaacatgcc cagcattcga ccggatcagc tatctcaact
    19801 gatgcagaca atgcgaacag ccagcattag cctttccaca cgctgcctgt tcatgtggca
    19861 acttcttact attacccgcc ctgccgaagc ggctgaagct cgctgggaag aagtagacat
    19921 agaagcgcaa gagtggaaga ttcccgcagc acgcatgaaa atgaaccgcg accatactgt
    19981 tccattgtca gatgaagcaa tagctgtgct ggagatgatg aaaccgctaa gcggcaaccg
    20041 agaatttatc tttcctagcc gtatcaagcc caaccagcct atgaacagtc agactgttaa
    20101 cgcctcacta aaacgcgcag gttttggtgg tgtgcttgtt tcgcatggtc tgagatcaat
    20161 tgccagtacg gctcttaatg aacaaggctt tccgcctgat gccattgagg cagcattggc
    20221 ccatgtggat aagaatgagg ttcgtcgtgc ttataaccgc agcgattacc tggaacagcg
    20281 tcgcccgatg atgcaatggt gggcaaattt tgtgatggca gcggatcgtg gaagcatgat
    20341 tgaaggtggg ataaaaggaa tgaagctagt tgggtgacta aatagctgcg cggcatagtg
    20401 tcccactttc agccagccct tgcaggttaa gccaccagac agtaaacggc aggtgtcgcg
    20461 ttattaggcg cagctattta gtagtaatat taggattggt gattctgcac tccagaagtt
    20521 cggtaatgct gccaacttac tgatttag
//
LOCUS       DAJVQH010000068        20474 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJVQH010000068 DAJVQH010000000
VERSION     DAJVQH010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.068"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            147..299
                     /gene="hokA"
                     /locus_tag="OQM87_002482"
     CDS             147..299
                     /gene="hokA"
                     /locus_tag="OQM87_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCS1508580.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(486..698)
                     /gene="cspA"
                     /locus_tag="OQM87_002483"
     CDS             complement(486..698)
                     /gene="cspA"
                     /locus_tag="OQM87_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCS1508581.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(979..1269)
                     /locus_tag="OQM87_002484"
     CDS             complement(979..1269)
                     /locus_tag="OQM87_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCS1508582.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1703..2413
                     /locus_tag="OQM87_002485"
     CDS             1703..2413
                     /locus_tag="OQM87_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCS1508583.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2463..3437)
                     /gene="ghrB"
                     /locus_tag="OQM87_002486"
     CDS             complement(2463..3437)
                     /gene="ghrB"
                     /locus_tag="OQM87_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCS1508584.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3541..4200)
                     /locus_tag="OQM87_002487"
     CDS             complement(3541..4200)
                     /locus_tag="OQM87_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCS1508585.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGVLSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4353..6686
                     /gene="bisC"
                     /locus_tag="OQM87_002488"
     CDS             4353..6686
                     /gene="bisC"
                     /locus_tag="OQM87_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCS1508586.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6655..7095)
                     /locus_tag="OQM87_002489"
     CDS             complement(6655..7095)
                     /locus_tag="OQM87_002489"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1508587.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7092..7655)
                     /gene="tag"
                     /locus_tag="OQM87_002490"
     CDS             complement(7092..7655)
                     /gene="tag"
                     /locus_tag="OQM87_002490"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCS1508588.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7813..8511
                     /locus_tag="OQM87_002491"
     CDS             7813..8511
                     /locus_tag="OQM87_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1508589.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8740..9948
                     /locus_tag="OQM87_002492"
     CDS             8740..9948
                     /locus_tag="OQM87_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1508590.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10272..11963
                     /gene="eptB"
                     /locus_tag="OQM87_002493"
     CDS             10272..11963
                     /gene="eptB"
                     /locus_tag="OQM87_002493"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCS1508591.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASAMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12055..12131
                     /locus_tag="OQM87_002494"
     tRNA            12055..12131
                     /locus_tag="OQM87_002494"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12089..12091,aa:Pro,seq:cgg)
     gene            12875..14482
                     /gene="dppA"
                     /locus_tag="OQM87_002495"
     CDS             12875..14482
                     /gene="dppA"
                     /locus_tag="OQM87_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCS1508592.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FESVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14790..15809
                     /gene="dppB"
                     /locus_tag="OQM87_002496"
     CDS             14790..15809
                     /gene="dppB"
                     /locus_tag="OQM87_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCS1508593.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15819..16721
                     /gene="dppC"
                     /locus_tag="OQM87_002497"
     CDS             15819..16721
                     /gene="dppC"
                     /locus_tag="OQM87_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCS1508594.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16732..17715
                     /gene="dppD"
                     /locus_tag="OQM87_002498"
     CDS             16732..17715
                     /gene="dppD"
                     /locus_tag="OQM87_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCS1508595.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17712..18716
                     /gene="dppF"
                     /locus_tag="OQM87_002499"
     CDS             17712..18716
                     /gene="dppF"
                     /locus_tag="OQM87_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCS1508596.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18746..20017)
                     /locus_tag="OQM87_002500"
     CDS             complement(18746..20017)
                     /locus_tag="OQM87_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1508597.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4903 a         5069 c         5381 g         5121 t
ORIGIN      
        1 actccaattg aatatcggaa tcagacctat atgcctcgtg tttaactgtc caactttttg
       61 gggtcagtac aaagtgaggc taccctatgc ctgaacacca taaggtgtgc ctcttactcg
      121 ttggaaatca acacaggggg ctgggaatgc cgcagaaata tagattactt tctttaatag
      181 tgatttgttt cacgctttta tttttcacct ggatgataag agattcactg tgtgaattgc
      241 atattaaaca ggagagttat gagctggcgg cgtttttagc ctacaaattg aaagagtaag
      301 agtcttcggc gggaaattat tcccgcctta cttacggcgt tgcgcattct cattgcaccc
      361 acatttattc ttcacaaaaa taataataga ttttattacg cgatcgatta tttatttcct
      421 gaaaaaaaat aaaaaaatcc ccgcaaaatg gcagggatct tagattctgt gcttttaagc
      481 agagattaca ggctggttac gttaccagct gccgggcctt tagcgccgct ttcgatggtg
      541 aaggacactt tctgaccttc gtccagagat ttgtaaccat agttctggat agcagagaag
      601 tgtacgaaca catctttaga gccatcgtca ggagtgatga agccgaagcc tttgtcagcg
      661 ttgaaccatt ttacgatacc agtcatttta ccggacatag tgtattacct ttaataatta
      721 agtgtgcctt tcggcgatat ggcgtgcttt acagattttg aagcgttaaa ggaatgtgca
      781 ctacgagggg tatcaacgat aactcttgaa gggacttgcc ttactacact gctttaatgg
      841 tctgtacgtc aaaccgttga tgtgcattaa gccactcatt ggcgggtgat gcaacaatta
      901 tttttcatat ttatgattaa tcggttggaa aacggtcctg tcatcaggac cgtaaacagc
      961 aataaagtgg ataaaagtct atttcatcaa ctgcttactt aatgccgggt tggcttgaat
     1021 caaacgcatc aattttagtt cggcacttga aggcttcacg cgtctggatt cccattcctt
     1081 taccatagcg actgatacgc ccaaaacccg ggcgaaatca tcgattttta atcctgtccc
     1141 ttttcgtaac cgctcaattt cggtacagga cgttgttctg tgcgtcaggg taattttctg
     1201 cgtttcatct ttaaaaacaa tctgttccag gctgctcaac agctcatgca ttggatcttt
     1261 atattccatt gaaaactcct caaatcacac tgcgggatcg tgaattgcat cgaagctcat
     1321 taagaatagt cgggaaaagc agacacagcg gggttaagtc agggcatgct ctggctatga
     1381 ttcgattcag cctgtcattt agcgtgactt atagccacct aacgatctga taaatgccga
     1441 tatatctctt atcactcctg ttttgtaaag atttcgcaaa tctaaccttc tgcgaaggcg
     1501 taaatctctg gctacaaaaa aggcaacggc actatcattt tttaacaatg aaacaacaaa
     1561 ttaaccttgt ttcattttct ccatgatttt accaggaaga ttacgtagaa agggtatcct
     1621 gcaagcctgt cctggactat ccttgtcacg tcagacacgc gtgtgtcgtt gtgcgctttt
     1681 tttgggtgaa aggagtaaga aaatggcgac aggaaagtcc tgctctcgct ggtttgcgcc
     1741 tcttgcggcg ttattaatgg tagttagcct gagtgggtgt tttgataaag aaggcgatca
     1801 gcgtaaagcg tttatcgact tcctgcagaa tacagtgatg cgtagcggtg aacgtctacc
     1861 aaccctgact gccgatcaga aaaaacagtt tggtcctttt gtctctgatt acgcgattct
     1921 gtatggttat tctcagcagg tgaatcaggc gatggattcc ggtctgcgcc cggtcgtaga
     1981 cagcgttaac gcaattcgcg taccgcagga ttacgttacg caaagcggtc cgctgcgtga
     2041 aatgaatggt tcgctgggtg tgctggcgca acaactgcaa aatgcgaagt tgcaggccga
     2101 tgctgcgcac tctgcgctaa aacagagcga tgacctgaaa ccggtatttg atcaggcatt
     2161 caccaaagtg gtaacgacgc cagctgatgc attgcaaccg ttgattccgg cggcacaaac
     2221 tttcacgcaa caactggtga tggttggaga ctacattgct cagcaaggta ctcaggtgag
     2281 ctttgtcgca aatggcatcc agttcccgac ttcacagcag gcaagtgaat ataacaaact
     2341 gattgcgcca ttaccggcac aacatcaggc gtttaatcag gcctggacta cagcagttac
     2401 tgcaacccaa taaagagtaa aagcccgagc gattatcggg ctttacttca cgcagtcgcg
     2461 gcttagtccg cgacgtgagg attcacacag ttcttctcaa cctttccttg taacgcatca
     2521 atcaaattat ccacggcaca ggcggccatg ccataacgcg tctcatgggt ggcagatcca
     2581 atatgcggta ctgcgacgac gttggccatt gagagcaacg gcgaatctac ggacagtggc
     2641 tcttgttcga agacatccag cccggcggcg tgaatttcgc ctttctgcaa tgctgcgatc
     2701 agtgcatttt cgtcaaccac cgggccacgt ccggcattaa tgaaaatggc ggaggatttc
     2761 attttggcga attgttctgc gccaaacaga tgatgcgtct catcagttaa cggcaggatc
     2821 aggcaaacga aatctgactc ttgtaacaga gtatccaaat cgcagtagcg ggcgttgaag
     2881 cgttcttctg cttctttatg gtggcggcgc gcgttataga ggatgggcat gttgaagcca
     2941 aagtgcgcac gttgtgccag cgccatgccg atccgtccca tcccgacaat gcccagtgtt
     3001 ttatggtgaa cgtcagtgcc gtaccagtcc gggcctatgc tcgcggtcca ttcgcctgct
     3061 tttacccgtt ctgctacctc cacaacccga cgagcggtag acaacaccag cgccatcagc
     3121 gtatcggcga cggtttctgt taatacggtt ggcgtgtgca tcagcagaat ttttcgggcg
     3181 gtaagcgcat cgacatcaaa attgtcatag ccgacggaga tcgttgatgt ggcacgcagt
     3241 ttcggcattt tttccagcaa tgcggcatct acattctcgt ttgaacccag taaaccttca
     3301 gcttctgcaa aaattgctgc attttgaacg acggtttgtg ggctgaggtt tgccacctgg
     3361 tgaacggtga aatgcgcttg caggcgttgc agtaaatcat caggtaaggc tttgtagagg
     3421 ataacggacg gcttcatgct tctctccatt cactgataag gcgatcacct tagcctgaaa
     3481 tatgatgaag gaaaactgaa gaatgccagg tgatttctgc atcacctggc atgaaaggga
     3541 ttacagcggg cttaaggtaa tttctacacg gcggttttgc gccttacctt ctgcggtgct
     3601 gttgctggcg attgggttag ccgggccaag gccctgagta cggatgcggc tggcgtccac
     3661 gccctgggtg atcaacgcgc tggcaacgga atccgcacgc tgctgggaga gacgcatgtt
     3721 caggtcgtga ccccccgtgc tgtcggtata accaatcacg ttaaccgccg ttttcggata
     3781 ctctttcagc accattgcca cgccggtcag ggtgttagcg cctgccggtt tcaggggcgc
     3841 gctgctgctg tcgaaggtca cattgttcgg catattgagg ataatgttat ccccgctgcg
     3901 ggttacgcta acaccagtgc cgcgcatttt gtcacgcagc ttcgcttcct gcacatccat
     3961 gtaataaccc acgccgccgc ccagagctgc gcctgctgct gcgccaatca gcgcgccttt
     4021 accgcgatct ttcttcgaag aagagagcac accaataccc gcgcccacga gagagcccag
     4081 acctgcgccg atagcagatt tgcctgcttc gcgttcgccg gtgtaagggt tagttgtgca
     4141 gccagatacc gccagagcgc cactcactac ggcggcaata agataaacac gtttcttcat
     4201 tgttaatcct taataacctt tttattcttt gccacgggtt ccgtggcggg agattatgcc
     4261 gcgtgaacat gaagattatt cctgggaata ctcggaaatt tgtaagtaat atttaactgc
     4321 tcaatacatc taacctttca ggagtcttcg gtttggccaa ctcatcctca cgatattccg
     4381 ttctgactgc cgcccactgg gggcccatgc tggttgaaac cgacggcgaa accgtgttta
     4441 gctcgcgtgg cgcgttagcc acaggaatgg aaaactcctt gcagagcgcg gttcgcgacc
     4501 aggttcacag caatacgcgg gtacgatttc caatggtgcg caaaggcttt ctcgcgtcac
     4561 cggaaaaccc gcaaggcatt cgtgggcagg atgaatttgt tcgcgtgagt tgggatgagg
     4621 cgctggatct tattcaccaa caacataaac gcattcgtga ggcttatggt ccggcatcga
     4681 tttttgctgg ttcctacggc tggcgttcaa acggcgtgct gcataaggcc tcgacattat
     4741 tacaacgcta tatggcgctg gcaggcggtt ataccgggca tctgggggat tattcgaccg
     4801 gtgcggcaca ggcgatcatg ccgtatgtcg taggtggcag cgaagtttat caacagcaaa
     4861 ccagttggcc gctggtgctg gaacatagcg atgtcgtggt gttgtggagt gctaacccac
     4921 tcaatacgct gaaaattgcg tggaatgcat ccgatgagca ggggctttct tacttttccg
     4981 cactgcgtga cagtgggaaa aagctgatct gcattgatcc aatgcgatcg gaaaccgtcg
     5041 atttctttgg cgataaaatg gagtgggtgg caccgcacat gggcaccgat gttgcgctga
     5101 tgctggggat cgcccatacg ctggtggaaa atggttggca cgacgaagcg tttctggcgc
     5161 attgcaccac aggttatgcc gtcttcgcct cttatttgct gggcgagagt gacggaatag
     5221 cgaaaaacgc cgaatgggcg gcagagattt gtggtgttgg cgcagcgaaa atccgcgagc
     5281 tggcggctat tttccaccaa aataccacca tgctgatggc aggctgggga atgcagcgcc
     5341 aacagtttgg tgagcaaaaa cactggatga tcgtcacgct ggcagcaatg ttggggcaaa
     5401 tcggcacacc cggcggcggt tttggtcttt cttaccattt tgccaatggt ggtaacccca
     5461 cgcggcgttc tgcggtgctc tcttccatgc agggcagctt gccgggtggc tgcgatgcgg
     5521 tggataaaat ccctgttgcc cgcattgttg aagcactgga aaaccctggt ggcgcatatc
     5581 aacacaacgg tatgaaccga catttcccgg atattcgttt tatctggtgg gcgggcggtg
     5641 ccaactttac tcatcatcag gataccaatc gtctgatccg tgcctggcaa aaaccggagc
     5701 tggtggtgat ctctgaatgc ttctggacgg cggcggcgaa acacgcggat atcgttctgc
     5761 ctgcgactac ctcttttgag cgtaatgatc tcaccatgac cggtgattac agtaatcagc
     5821 atctggtgcc gatgaagcaa gtggtgccgc cacgctatga agcgcgtaat gattttgatg
     5881 tttttgccga gttgagtgaa cgttgggaga agggcggcta tgcgcggttt acggaaggaa
     5941 aaagtcagct gcaatggctg gaaacgtttt ataacgttgc ccggcagcgc ggggcaagcc
     6001 agcaggttga attgccgcca tttgctgagt tctggcaagc caaccagtta attgagatgc
     6061 cggaaaaccc ggacagcgag cggtttattc gcttcgctga tttttgccgc gatccgctgg
     6121 cgcatccgtt aaaaaccgcc agcggcaaga ttgaaatctt ctcacagcgt attgccgatt
     6181 acggttaccc ggattgccct gggcatccaa tgtggctgga gccggacgaa tggcagggca
     6241 atgccgaacc agaacagttg caggtacttt ctgcccatcc ggcgcaccgt ctgcacagcc
     6301 agctgaatta cagttctctg cgcgaattgt acgcggtagc aaaccgtgag cctgtcacca
     6361 ttcatcctga cgatgctcag gcgcgcggca taaccgaagg ggatatggtg cgggtgtgga
     6421 actcccgtgg gcagatcctt gctggagcgg tcattagcga gggaattaaa cctggcgtga
     6481 tttgcattca cgaaggggca tggccggatc tggatttaac cgctgacggt atttgtaaaa
     6541 acggcgcagt gaacgtgctg accaaagatc tccccagctc gcggctgggg aatggctgtg
     6601 cgggtaatac ggcgctggca tggctggaaa aatacaacgg tccggaactg acacttacag
     6661 cgtttgaacc accggccagc tcataatcca ggtgggtagt tgggtttcat cctgccatgc
     6721 gcaatcgaca atgtgaaaac cctgcgcacg gtaaaaatct atcgccggtt gatttttttg
     6781 ataaacctcc agcatcagat ggggatagcg ctgctgcaca tactgcatca gcgccttacc
     6841 aataccgcgt ctgacggcct tcggtgcgac aaacattgct gccagaaatc ggccttccat
     6901 aatgctgaca aaaccgagaa gcttaccgtc ctcttcccag acccagtttt gcgcgttggc
     6961 aagataggca tcccgcacca gcggaatgca gtcacgccag taattcgctt ttataaaggg
     7021 atgcccccag gttgtacttt ccagccacag ttcgaggatc gcggggagtt ctgaacgttg
     7081 cgcttcccga atcatggttt atttcccgga tagcaacagc agccaaccac atgatcattc
     7141 accagcccac atgcctgcat aaaggagtaa cagattgtgg tgccgacaaa cttaaaacca
     7201 cattttttca gtgccttaga tagggcgtcg gaggcggacg tagatgtggg aatttcgctc
     7261 aacgttgtgg cttgtgtcac ctgtggctga tgatttacaa acgaccagac aaagtcggga
     7321 aacggttcgc cgttctgttc catttgcagg tacgcccgcg cattaccaat aattgcctga
     7381 attttccctc gatggcggat aatcccggcg tcctgtacca gtctttcgac atcctcttcc
     7441 tgcattgctg cgaccttcac cggatcgaac tgatgaaagc aggcgcgata gttttcgcgt
     7501 tttttgagga cggtgatcca cgataaccca gcctgctgcc cttcaaggca gatcatttcg
     7561 aacagttttt tactgtcagt ttcaggcacg ccccactcat tatcatggta ggcaatataa
     7621 agcgggccct gactcaccca gccgcaacgt tccatacttt ccctcgctat gtacccgatc
     7681 gtaaaaattt cagccgcttt tcttgacgca tcggtgaaaa agtcaatatt taatacaggg
     7741 ctactatgcc cgatatcctt catacaatga caataatatt gccgaattcg gctcttccct
     7801 ggagtcgtgt taatgatcat aaaaaaaagc ggtggtcgct ggcagctaag cctgctggcg
     7861 agcgtggtaa tcagtgcctt ttttctcaac acagcttacg cctggcaaca agaatatatc
     7921 gttgatacgc aacccggaca ttccacagag cgttacacct gggatagtga tcatcaacct
     7981 gattacaacg atattttgtc gcaacgtatt caaagtagcc aaagggcgct gggactggaa
     8041 gtcaatctgg cggaagaaac tcctgtggat gtgaccagca gtatgagtat gggctggaat
     8101 tttcctttat atgaacaggt tacaaccggc ccggtcgcgg cattacatta cgatggcaca
     8161 accacctcga tgtataacga gtttggcgac agtactacca cgctgaccga tccgttatgg
     8221 catgccagcg tgagtagctt aggctggcgt gttgactccc ggcttggcga tctccgaccc
     8281 tgggcgcaaa tcagctataa ccagcaattt ggcgagaata tctggaaggc gcaatcaggc
     8341 ctgagccgga tgacggcgac aaaccagaac ggcaactggc tggatgtcac cgtaggcgct
     8401 gatatgttgc tcaatcaaaa tattgccgcc tatgctgcgc taactcaggc agaaaatacc
     8461 actaataata gcgactatct gtatacgatg ggggttagcg ccagatttta acgtaacagt
     8521 cacaattgaa accattgaat aacaacagtt gtggcgatag tgggtgctaa cttaccaaat
     8581 aataaatttg gtgaataatt gtcgcgtcat tcattcctga actaaggcat ttcattccgt
     8641 tctgatggca tttcatgccg tttttcccca agcataaaat gcacttcgtt atggttgtcg
     8701 gcagagattt ttccttttta ttactgcagg aatactgcca tgacaccttc aaattatcag
     8761 cgtacccgct ggctgacact catcggtact atcattaccc agtttgcgct ggggtcggtt
     8821 tatacctgga gcctgtttaa tggcgcgctt tctgccaagc tggatgcgcc ggtaagccag
     8881 gtcgctttct ctttcggctt gttaagtctg gggctggcaa tttcgtcttc tgttgcgggc
     8941 aaattgcagg aacgttttgg cgttaaacgc gtcaccgtgg cttccggcat tttgctggga
     9001 ttaggcttct tcctgaccgc gcattccaac aacctgatga tgctgtggtt aagcgccggt
     9061 gtgctggtcg gtctggcgga tggcgcgggt tacctgctga cgctctctaa ctgcgtgaag
     9121 tggttcccgg agcgtaaagg tctgatctcc gcgttcgcta tcggttctta tggtctgggt
     9181 agcctgggtt tcaaatttat cgacacgcag ctgctcgaaa cggtgggtct ggaaaaaacc
     9241 tttgtgattt ggggagcgat tgcgctggtg atgattgttt tcggcgcaac gttaatgaaa
     9301 gacgcaccaa aacaggaagt gaaaaccagc aatggtgtgg tggagaaaga ttacacgctg
     9361 gcagagtcga tgcgtaaacc gcagtactgg atgttagcgg taatgttcct gactgcctgc
     9421 atgagcggcc tgtacgtgat tggggtagcg aaagatatcg cccaaagtct ggcacacctt
     9481 gatgtggttt ccgcagccaa tgcagtaacg gttatttcca tcgctaacct ttcaggtcgt
     9541 ctggtgctgg gcattctgtc tgacaaaatt gcccgtatcc gtgttatcac cattggtcag
     9601 gtgatttcgc tggtgggtat ggcggccctg ctgtttgcac cattaaatgc agtgacgttc
     9661 tttgcagcga ttgcctgcgt ggcatttaac tttggcggca ctattaccgt ctttccatca
     9721 ctggtcagtg agttcttcgg cctcaataac ctggcgaaaa actacggtgt gatttacctc
     9781 ggtttcggta tcggtagcat ttttggttcg attatcgcct cactgtttgg cggtttctat
     9841 gtgaccttct acgtgatttt cgccctgctg attctgtcat tggcgctttc tacgacgatt
     9901 cgtcagccag agcagaaaat gttgcgtgag gcgcatggct ccctttaatg ctcagccgcg
     9961 agctactggg cttcagcttc cgtgtgcatc aaacgcatca acgaaatgta taaacaatga
    10021 ctcgtaaaat ttgggccagt taactggccc tttttactgc tttttgtaac gctaaagtct
    10081 cttttcaaac ttgcattttt gtaaatttgt gcttcatgca cactctttcc ccacactttt
    10141 tcgctttgct gtggtctact tattcgcgcg tgtagatttt acttatctga ctacctccgc
    10201 acttttcccc tgccgggcct gaaaagccgc taagcagggt gttatcacct gtttgtccag
    10261 ggtttgtttg catgagatac atcaaatcga ttacacagca gaagctgagc tttttgcttg
    10321 caatctatat tggccttttt atgaatggcg cggtttttta tcgccgcttc ggcagctatg
    10381 cgcacgattt taccgtctgg aaaggcgttt ctgctgttgt tgaactggcc gccaccgtac
    10441 tggtgacctt ctttttacta cgtcttctat cgctgtttgg ccgccgcagc tggcgtattc
    10501 tggcgtcgct ggtggtgctc ttttccgcag gtgccagcta ttacatgacc ttccttaatg
    10561 tggtcattgg ttatggcatc atcgcttccg ccatgaccac cgatatcgac ctgtcaaaag
    10621 aagttgttgg tctgaacttt attctctggt taatcgccgt tagtgcattg cctcttatcc
    10681 ttatctggaa taaccgctgt cgctacacct tgctccgaca actgcgaacc ccagggcagc
    10741 gtattcgcag cctggtggtc gtcgtactgg cgggtattat ggtttgggca ccgattcgtt
    10801 tgctggatat ccagcagaag aaagtggaga gggcgaccgg cgttgatttg ccgagttatg
    10861 gcggtgtcgt agcgaactct tatctgccat caaactggct ttctgcgttg gggctgtatg
    10921 cctgggcgcg ggtcgatgaa tcttccgata ataattcatt gcttaatccg gcgaagaagt
    10981 ttacatacca ggctccacaa aacgttgatg atacttatgt cgtgtttatc atcggtgaaa
    11041 ccacgcgttg ggaccatatg ggtattttcg gctatgagcg taataccacg ccgaaactgg
    11101 cccaggagaa aaatctggcg gcgttccgtg gttactcctg tgataccgca accaaactct
    11161 cactgcgttg catgtttgta cgtcaggggg gcgcggaaga taatccgcag cgcacattaa
    11221 aagaacagaa cattttcgcg gttctgaagc agttaggatt cagttctgac ctctacgcta
    11281 tgcagagcga aatgtggttc tacagcaaca cgatggcaga caacattgct tatcgtgagc
    11341 agattggtgc ggagccacgt aatcgtggca agccggtaga tgatatgttg ctggtagacg
    11401 aaatgcagca atcgctaggg cgcaacccgg atggtaagca tctgatcatt ctgcatacca
    11461 aaggttcgca ttttaactac acccagcgtt atccgcgtag cttcgcgcag tggaagccgg
    11521 aatgtattgg tgttgatagc ggctgtacca aagcgcagat gatcaactcc tatgacaact
    11581 cggtgaccta tgtggatcac tttatctcca gcgtgattga tcaggttcgc gataagaaag
    11641 cgattgtgtt ctacgcagct gaccacggtg agtcaattaa tgaacgcgag cacctgcacg
    11701 gcacgccgcg tgaactggca ccgccggagc agttccgcgt accgatgatg gtctggatgt
    11761 cagataaata tctggaaaat ccggccaatg cgcaggcgtt tgcgcagctg aaaaaagaag
    11821 ccgacatgaa agtgccacgc cgtcacgtag agctgtacga caccatcatg ggttgtcttg
    11881 gctatacttc accggatggt ggaattaacg aaaacaacaa ctggtgtcac atcccgcaga
    11941 caaaagtggc agcggctaac taagcggcct gctgactttc tcgccgatca aaaggcattt
    12001 tgctattaag ggattgacga gggcgtatct gcgcagtaag atgcgccccg cattcggtga
    12061 ttggcgcagc ctggtagcgc acttcgttcg ggacgaaggg gtcggaggtt cgaatcctct
    12121 atcaccgacc aaattcgaaa agcctgctca gcgagcaggt ttttttgcat ttgtagctca
    12181 tgaggatgag aacctccggg ggcagggagg ctcgactcga cgtaggcctg ataagacgcg
    12241 ccagcgtcgc atcaggcaac ggcaccgcac ttattgtcgg atgcggcgcg accgccttat
    12301 tcgaccaaca caccacacat tttcctgcca actttgcgcc ccatatctca ctatccatta
    12361 acggatttgt gacagaaacc attgtctttt tttatatttg cttaaatctt ttttgcgtta
    12421 cttatggctg gcgctggcat tttccgctgt gcgttttagc gttcatggta ttaatcgctc
    12481 agatatccac cttttcctca gatttttttc attaaatgca taaaacttaa tcaccaaatt
    12541 ttgctaaata ttaagctgat tgttctggtt atgttggagt agcacaaatt tctctgctgc
    12601 aaatagctgt ttgaagtttt tttaatcttt gtttgtggat tctcaccgtt ggtgtaaatc
    12661 ccggttggct gtattgacgt tttcacattc tgttgacaga ttgtaggtca cgaggggcat
    12721 tttatggagg atccgcactg ttacactgat gttaattagt acggcatccc cacctcataa
    12781 cgttgacccg accgggcaaa aaacaaaaaa ggtcaggcag cgacaaccca ctgcaaaggg
    12841 ttaaaacaac aaacatcaca attggagcag aataatgcgt atttccttga aaaagtcagg
    12901 gatgctgaag cttggtctca gcctggtggc tatgaccgtc gcagcaagtg ttcaggctaa
    12961 aactctggtt tattgctcag aaggatctcc ggaagggttt aacccgcagc tgtttacctc
    13021 cggcaccacc tatgacgcct cttccgtccc gctttataac cgcctggttg aatttaaaat
    13081 cggcaccacc gaagtgatcc cgggcctcgc tgaaaagtgg gaagtcagcg aagacggtaa
    13141 aacctatacc ttccatctgc gtaaaggtgt gaagtggcac gacaataaag aattcaaacc
    13201 gacgcgcgaa atgaacgccg atgacgtggt gttctcgttc gatcgtcaga aaaacgcgca
    13261 aaacccgtac cataaagttt ctggcggcag ctacgaatac ttcgaaggta tgggcttgcc
    13321 ggagctgatc agcgaagtga aaaaggtgga cgataacacc gttcagtttg tgctgactcg
    13381 accggaagcg ccgttcctcg ctgacctggc aatggacttc gcctctattc tgtcaaaaga
    13441 atatgctgac gcgatgatga aagccggtac accggaaaaa ctggacctca acccaatcgg
    13501 aaccggtccg ttccagttac agcagtacca aaaagattcc cgtatccgct acaaagcgtt
    13561 tgatggctac tggggcacca aaccgcagat cgatacgctg gtcttctcta ttacccctga
    13621 tgcttccgtg cgttacgcga aattgcagaa gaatgaatgc caggtgatgc cgtacccgaa
    13681 cccggcagac atcgctcgca tgaagcagga taaatccatc aacctgatgg aaatgccggg
    13741 gctgaacgtc ggctacctct cgtataacgt gcagaaaaaa ccgctggatg acgtgaaagt
    13801 tcgccaggca ctgacctacg cggtgaacaa agacgcgatc atcaaagcgg tttatcaggg
    13861 cgcgggtgta tcagcgaaaa acctgatccc gccaaccatg tggggctata acgacgacgt
    13921 tcaggattac acctacgatc ctgaaaaagc gaaagccttg ctgaaagaag cgggcctgga
    13981 aaaaggtttc tccatcgacc tgtgggcgat gccggtacaa cgtccgtata acccgaacgc
    14041 tcgccgcatg gcggagatga ttcaggcaga ctgggcaaaa gtgggcgtgc aggccaaaat
    14101 cgtcacctac gaatggggtg agtacctcaa gcgtgcgaaa gatggcgaac atcagacggt
    14161 aatgatgggc tggactggcg ataacgggga cccggataac ttcttcgcca ccctgttcag
    14221 ctgcgccgcc tctgaacaag gttctaacta ctcaaaatgg tgctacaaac cgtttgaaga
    14281 tctgattcaa ccggcgcgtg ctaccgacga ccataacaaa cgcgttgaac tgtacaagca
    14341 agcgcaggtg gtgatgcacg atcaggctcc ggcactgatc atcgctcact ccaccgtgtt
    14401 tgaatcggta cgcaaagaag tcaaaggcta tgtggttgat ccattaggca aacatcactt
    14461 cgaaaacgtc tctatcgaat aattaaaagc catacaagac tgatggcaaa ggcaaaactg
    14521 cctgatgtgc tccgcttatc aggcctacga aaattctgca atgtattgaa tttgcacgat
    14581 tttgtaggcc ggataaggcg ttaacgccgc atccggcata aacaaagcgc actttgtcaa
    14641 caatctgtat acccggtggc gctgtgcctc tgcgtgcagc gccatccggc agcaatactt
    14701 cattctctgt ccgacaaggg cgggaattga tttgtgagca atacagacac gcagttccag
    14761 gctgcgggtc actatagaga atccgggtta tgttgcagtt tattctccga cgtttgggac
    14821 tcgtcatccc cacgtttatc ggtattaccc ttctcacatt tgcctttgtc cacatgatcc
    14881 cgggcgatcc ggtgatgatc atggcaggcg aacgtgggat ctcccctgag cgtcacgtgc
    14941 agctgctggc tgaactcggc ttagataaac cgatgtggca gcagtatctc cattacattt
    15001 ggggcgtaat gcacggcgat ctaggcattt caatgaaaag ccgaattcca gtatgggaag
    15061 agttcgtgcc gcgctttcag gccacgctgg aacttggcgt ctgcgcgatg atttttgcta
    15121 cggcagtcgg tattccggtt ggtgtgctgg ctgcggttaa acgcggttcc attttcgatc
    15181 acactgcagt tggcctggcg ctgaccggct actcgatgcc gatcttctgg tggggcatga
    15241 tgctgatcat gctggtttcg gtgcactgga acctgacgcc cgtctccggt cgcgtgagcg
    15301 atatggtgtt cctcgatgac tccaatccgt taaccggttt tatgctaatc gacaccgcca
    15361 tctggggtga agacggcaac tttatcgatg ccgtcgccca tatgatcttg cccgccattg
    15421 tgctgggtac tattccgctg gcggtcattg tgcgtatgac acgctcctcg atgctggaag
    15481 tgctgggcga ggattacatc cgcaccgcgc gcgccaaagg gctaacccgc atgcgggtga
    15541 ttatcgtcca tgcgctgcgt aacgcgatgc tgccggtagt gaccgttatc ggcctccagg
    15601 tgggaacatt gctggcgggg gcgattctga ccgaaaccat cttctcgtgg cccggtctgg
    15661 ggcgctggtt gattgacgca ctgcaacgcc gcgactatcc ggtagtgcag ggcggcgtat
    15721 tgctggtggc gacgatgatt atcctcgtca acttgctggt cgatctgctg tacggcgtgg
    15781 tgaacccgcg tattcgtcat aagaagtaag gggacatcat gtcacaggtt actgaaaata
    15841 aagtgattag cgcaccggtg ccgatgaccc cgttacagga gttctggcac tattttaaac
    15901 gcaacaaagg cgcggtcgtc gggctggttt acgtcgccat cgtgctgttc atcgcgatct
    15961 ttgccaactg gattgcgcct tataacccgg cggatcagtt ccgcgatgcg ctgctcgccc
    16021 cgccagcctg gcaggaaggc ggcagtatgg cacacttgct gggcaccgat gacgtcggtc
    16081 gtgatgtgct gtcgcggctg atgtacggcg cgcgtttatc gctgctggtt ggctgtctgg
    16141 tggtggtgtt gtcgctgatt atgggcgtta ttctcggcct gatcgccggt tacttcggcg
    16201 gcctggttga taacattatc atgcgcgtgg tcgatatcat gctggcgctg ccaagtctgc
    16261 tgttggcact ggtgctggtg gcgattttcg gcccgtcgat tggtaacgcc gcgctggcgc
    16321 tgaccttcgt tgccttgccg cactatgtgc gcttaacccg cgccgccgtg ctggtggagg
    16381 tgaatcgcga ttacgtcacc gcctctcgcg tggcaggtgc cggggcgatg cgccagatgt
    16441 ttattaacat cttcccgaac tgccttgcgc cgctgattgt tcaggcgtcg ctcggtttct
    16501 ctaacgccat tctcgatatg gctgctcttg gtttcctcgg catgggggca cagccgccaa
    16561 cgcctgagtg gggcaccatg ctctccgacg tgttgcagtt cgcgcaaagc gcctggtggg
    16621 tcgtgacctt ccctggtctg gcgatcctgt tgacggtgct ggcatttaac ctgatgggtg
    16681 acggtctgcg tgacgcgctc gatcccaaac tgaagcagta agaggttcga gatggcgtta
    16741 ttaaatgtag ataaattatc ggtgcatttc ggcgacgaaa gcgcaccgtt ccgcgccgta
    16801 gaccgcatca gctacagcgt aaaacagggc gaagtggtcg ggattgtggg tgagtccggc
    16861 tccggtaagt cggtcagttc actggcgatt atggggctga ttgattatcc gggccgcgta
    16921 atggcagaaa aactggagtt taacggccag gatttgcagc gtatctcaga aaaagagcgc
    16981 cgcaacctgg tgggtgccga agtggcgatg atcttccagg acccgatgac cagccttaac
    17041 ccatgctaca ccgtgggttt ccagattatg gaagcgatta aagtgcatca gggcggcaac
    17101 aaaagtaccc gccgtcagcg ggcgattgac ctgctgaatc aggtcggtat tcccgatccg
    17161 gcttcgcgtc tggatgttta cccgcatcag ctttccggcg gcatgagcca gcgcgtgatg
    17221 atcgccatgg cgattgcctg tcggccaaaa ctgctgattg ccgatgaacc gaccaccgcg
    17281 ctggacgtga ccattcaggc gcaaatcatc gaactactgc tggagctaca gcagaaagag
    17341 aacatggcgc tggtgttaat tacccatgac ctggcgctgg tggcggaagc tgcgcataaa
    17401 atcattgtga tgtatgcagg tcaggtagta gaaacgggcg atgcacacgc catcttccat
    17461 gcgccgcgtc acccgtatac tcaggcattg ctgcgtgcgc tgccagaatt tgctcaggac
    17521 aaagaacgtc tggcgtcgtt gccaggtgtt gttcccggca agtatgaccg cccgaacggc
    17581 tgcctgctta acccgcgctg cccctatgcc actgacagat gtcgcgctga agaaccggcg
    17641 ctgaatatgc tcgctgatgg gcgtcagtcc aaatgccatt acccacttga tgatgccggg
    17701 aggcccacac tatgagtacg caagaggcca cctcgcaaca accgctgttg caggctatcg
    17761 acctgaaaaa acattatccg gtgaagaaag gcatgttcgt gccggaacgt ctggtgaagg
    17821 cgctggacgg cgtttcgttt aaccttgaac gtggcaaaac gctggcagta gtgggcgaat
    17881 ctggctgcgg taaatcgacc ctcggtcggt tgctgacgat gatcgaaacg cccaccggcg
    17941 gcgagctgta ttatcagggg caggatctgc tcaagcacga tccgcaggcg cagaagctgc
    18001 gtcggcagaa aatccagatc gtcttccaga atccctacgg ttcgctgaat ccgcgtaaga
    18061 aagttgggca aattctcgaa gagccgctgc ttattaacac cagcttaagc aaagaacagc
    18121 gtcgggaaaa agccctgtcg atgatggcaa aagtcggcct gaaaaccgag cactatgacc
    18181 gctatccgca catgttttcc ggcggacaac gtcagcgtat cgctattgcc cgtggtctga
    18241 tgctcgaccc ggatgtggtg attgccgatg agccggtttc tgcgctggac gtctcagtgc
    18301 gtgcgcaggt gctgaatctg atgatggatt tgcagcagga gttggggctt tcttatgtct
    18361 ttatctccca cgacctgtcg gttgtggagc acattgctga tgaagtgatg gtgatgtacc
    18421 taggccgctg cgtggagaaa ggaacgaagg accaaatctt caataacccg cgccatccgt
    18481 acacccaggc gctgctctcc gcgacgccac gcctgaaccc ggacgatcgt cgtgaacgca
    18541 tcaaactcac cggtgaactg ccaagcccgc tgaatcctcc gccgggttgc gccttcaacg
    18601 cccgctgtcg tcggcgcttc ggcccctgca cccagttgca gccgcagcta aaagactacg
    18661 gaggtcaact ggtagcttgt tttgctgttg atcaggatga aaatccgcag cgttaactca
    18721 caaaaccggg gccatgcgct ccggtttact caatcagctt caacagtggc gacaagcata
    18781 gcaaaatgcc gtaaagcaga atcagccagg ctttaaatcc gcgaagtttt tccagttgtg
    18841 cgactttata gatcaggaaa aacggaatga ggcacgacac aatgccatat aacgggcttc
    18901 ccaactgaaa gaacaccagc actgatacac gaaacgaaac ccaaatcgtc aacgtaatga
    18961 cgataaaagc gcagatcgcc agagtcagca tgcgtgagtt aattttcttg gtatcaataa
    19021 ttcggcttaa cagattgaga ataatgcctt taatagcctc gtggaaaccg agataaatgc
    19081 caaagaatgc ggtcagtacg gcaaagatat tcagcaccgt agaggtgata tgaatgatat
    19141 gtccagggat cacctgcgcg gccagtgcca gtgccgagat attttgttca aaggcagaaa
    19201 cggcttcttc gtggctaatt gagaaggtaa acgaaaaggc aaaaaacagg atcaccgcga
    19261 tgagcgtgat ataactaatt cggtgggtac gcagcgcgag ccgggttgcc agtactttat
    19321 ctgcttcccg tttacgatag gcaatattca ttgggttaag tacctgaata aataccgcag
    19381 aaaagaagca aaatggaatg gtaagcagga catcgcggaa aaagacggag gcttgcggga
    19441 aggcggttat attggcgaaa ttccagtgcg ggatcatcgc aaaaccgaac acgacaataa
    19501 tccctacttt gaccaccacc attggcccag aaatcttaaa cagtaatcgt tcaccaccag
    19561 acgcaatcgc tatcagtacg gcgaaaatag cgactttata aagtagagat tgtgaaagat
    19621 cggcatcggt taaaccgaag gttttcaggt acgaggcgct gtcgaaaacc acggagagag
    19681 agtagataaa aatcccgtgg ataatcatca aaaagtagat aaccccgagg aaaattcccc
    19741 agttcttccc caggtaatga ctgataatat cggtgtagtc attacaggaa tcgctttcag
    19801 aaagggtttt taaataaatg tcctgcacta cccaggtggc aggataagca atgatcgctg
    19861 cggtaataaa tacccaaatt cccttcaagc caatttgtac tggcatcagc acggttccgg
    19921 caccaatcgc catgcctatg cataataaaa cccagccgaa gtcgtagcgt gtaaacggca
    19981 atttctgatt gtgtttcgat agtgtgttgt gctgcataaa gtcctgtcca gaaagaagtc
    20041 ggcctcgcaa aaatgaataa acccagtttc tcttgcgcaa caggtggaga agtcgaatac
    20101 gcagtatgta aaaaatgagg caacagattc actggggagg tcgcgaggag gcgcaattct
    20161 acaaaattgc gcatttcact accagtctgg agtcagcccg gatatgaact gcaactattg
    20221 caaataaaga aattaagctg gaaggcatcg cgcaatgagg aagattgccg ggtgctgaca
    20281 gtaaaaagca atcgtaaaat cagcggcaag ccggaagggt tccggtgagg cgcaatgttg
    20341 cgggggcttt atccctggtg gcattggttg ctggagagag aaaacccccg cacgttgcag
    20401 gtatgtacct gacaacataa cgggggctaa tcttgaacct agaccactca agaatagccg
    20461 cgaaacgttg tcat
//
LOCUS       DAJVQH010000069        20129 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJVQH010000069 DAJVQH010000000
VERSION     DAJVQH010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.069"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(66..4526)
                     /gene="mukB"
                     /locus_tag="OQM87_002501"
     CDS             complement(66..4526)
                     /gene="mukB"
                     /locus_tag="OQM87_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCS1508598.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGRDEAPSQAS"
     gene            complement(4526..5230)
                     /gene="mukE"
                     /locus_tag="OQM87_002502"
     CDS             complement(4526..5230)
                     /gene="mukE"
                     /locus_tag="OQM87_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCS1508599.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5211..6533)
                     /gene="mukF"
                     /locus_tag="OQM87_002503"
     CDS             complement(5211..6533)
                     /gene="mukF"
                     /locus_tag="OQM87_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCS1508600.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6530..7315)
                     /gene="cmoM"
                     /locus_tag="OQM87_002504"
     CDS             complement(6530..7315)
                     /gene="cmoM"
                     /locus_tag="OQM87_002504"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCS1508601.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7451..8230
                     /gene="elyC"
                     /locus_tag="OQM87_002505"
     CDS             7451..8230
                     /gene="elyC"
                     /locus_tag="OQM87_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCS1508602.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8207..9100)
                     /locus_tag="OQM87_002506"
     CDS             complement(8207..9100)
                     /locus_tag="OQM87_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCS1508603.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9254..10000)
                     /gene="kdsB"
                     /locus_tag="OQM87_002507"
     CDS             complement(9254..10000)
                     /gene="kdsB"
                     /locus_tag="OQM87_002507"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCS1508604.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9997..10179)
                     /gene="ycaR"
                     /locus_tag="OQM87_002508"
     CDS             complement(9997..10179)
                     /gene="ycaR"
                     /locus_tag="OQM87_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCS1508605.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10231..11463)
                     /locus_tag="OQM87_002509"
     CDS             complement(10231..11463)
                     /locus_tag="OQM87_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCS1508606.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11500..12486)
                     /gene="lpxK"
                     /locus_tag="OQM87_002510"
     CDS             complement(11500..12486)
                     /gene="lpxK"
                     /locus_tag="OQM87_002510"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCS1508607.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12483..14231)
                     /gene="msbA"
                     /locus_tag="OQM87_002511"
     CDS             complement(12483..14231)
                     /gene="msbA"
                     /locus_tag="OQM87_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCS1508608.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14268..16487)
                     /locus_tag="OQM87_002512"
                     /pseudo
     CDS             complement(14268..16487)
                     /locus_tag="OQM87_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16694..16978)
                     /gene="ihfB"
                     /locus_tag="OQM87_002513"
     CDS             complement(16694..16978)
                     /gene="ihfB"
                     /locus_tag="OQM87_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCS1508609.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17138..18811)
                     /gene="rpsA"
                     /locus_tag="OQM87_002514"
     CDS             complement(17138..18811)
                     /gene="rpsA"
                     /locus_tag="OQM87_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCS1508610.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18922..19605)
                     /gene="cmk"
                     /locus_tag="OQM87_002515"
     CDS             complement(18922..19605)
                     /gene="cmk"
                     /locus_tag="OQM87_002515"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCS1508611.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19778..>20129)
                     /gene="ycaL"
                     /locus_tag="OQM87_002516"
     CDS             complement(19778..>20129)
                     /gene="ycaL"
                     /locus_tag="OQM87_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCS1508612.1"
                     /translation="VALGHSLAEMKASYAIVAARDAISATSGVASQLSRSQLGDIAEG
                     AINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASLDGGRTQSMFDSHPPST
                     ERAQHIRDRIASGK"
BASE COUNT         4825 a         5582 c         4913 g         4809 t
ORIGIN      
        1 cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt gctgccgcct
       61 taattttaac tcgcctggga aggcgcttcg tctcttcctg gaagcgtttc agggagttgc
      121 ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa gactttacgt
      181 accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc gatgatgagc
      241 tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc atccagtcgc
      301 gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg caggcggcga
      361 gattcatctt cccagctttg taccaccatc accagaatcg acataccggt accaatcgcc
      421 tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc acggttaacc
      481 tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat ggtctgcggc
      541 gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc caacgcttcc
      601 gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg ttcagagagc
      661 acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac gctgttcacc
      721 tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt ctgctcgcgc
      781 tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag tttctgttca
      841 cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc gatagcttcc
      901 accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg ctgataaacc
      961 gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat gcgcagcacg
     1021 tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc cttatccgac
     1081 atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa gcggcgctca
     1141 acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc ggtcactacc
     1201 tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt caggttgtcc
     1261 atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga acggttattg
     1321 ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc cccgctatca
     1381 gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc ttttttggtg
     1441 tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg cgctgcgtga
     1501 ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg actgttccag acgttcacgc
     1561 agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga atagctaaag
     1621 tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc atcgcgctgc
     1681 atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg gtcgctctgc
     1741 aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg aacaaaacgc
     1801 gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc cgccaggctg
     1861 tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac gccttctttc
     1921 gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact caacgcccgc
     1981 tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc agactcaaac
     2041 gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg ctgggttttc
     2101 tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc ggcatggagg
     2161 ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg gaaacgtgaa
     2221 taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc atcaacgctg
     2281 aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc ttccgggcaa
     2341 tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac gatggcgtgg
     2401 cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac gtcgtcataa
     2461 atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg ctgatcttca
     2521 gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt gcgcgcgcct
     2581 acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg cagatattca
     2641 gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg gttgagactg
     2701 ttttgcgctg ccagccaaac cggtgcacgc agcatcaaac tctgaatgcg agactgcagc
     2761 tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac gctatcggaa
     2821 agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc aatatcaaaa
     2881 ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg ctgctcacgc
     2941 agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc tgccaggtga
     3001 cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc gttacgcgcc
     3061 agcgggccgt tgattgccac caccagctga taagcctgct caaactggct gtgcgcggtt
     3121 tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc cagctctttc
     3181 gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg cagatggcac
     3241 agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc gcgcgtttgc
     3301 tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc cacttccagt
     3361 tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc tgccaccact
     3421 tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta gcgttcgatc
     3481 ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc cgcctgataa
     3541 tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc catatcgacg
     3601 tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg acgaaactcc
     3661 agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc cgccgccacg
     3721 tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg ggtgacacga
     3781 atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg gaacgcttta
     3841 cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat ggcactggag
     3901 atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc agatgcggag
     3961 cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc ggtaatggag
     4021 ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag ctcgttaagc
     4081 ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt cggctgcacc
     4141 gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc gcgtccggca
     4201 acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat ggtgtcgagc
     4261 atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg cgaaccgctg
     4321 gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg gatcagcgcc
     4381 gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga aagcgtcgtg
     4441 accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat cagcgtcagt
     4501 gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc tgactctctt
     4561 cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca tcacgaatca
     4621 ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg aagcggaaca
     4681 ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc atgcctaaac
     4741 gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg tcaacgtctg
     4801 aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc agggtgagca
     4861 gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt tccgggctga
     4921 gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag acggaacgcg
     4981 ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg cgaataagct
     5041 caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc aggaatgcat
     5101 gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg tccagcgccg
     5161 gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt tcaatatttg
     5221 tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca tttcgctggc
     5281 agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat aacaatacgc
     5341 gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac caccagacca
     5401 agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat gatcgccgcc
     5461 agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag ttccccagtc
     5521 acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg atcggcattg
     5581 gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg taaccgctga
     5641 gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg tacgtggcgg
     5701 tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg atcgagtttg
     5761 ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt catcgtcgca
     5821 tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag cgtatcctgc
     5881 aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga aatcgccgcc
     5941 cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg ctgttcgtcc
     6001 atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata tttcagtggc
     6061 gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc atctgctgcg
     6121 cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt agaaaactcg
     6181 cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg gtaaattgcg
     6241 ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac catgtcgttg
     6301 atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc atcactcaca
     6361 tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg ctcgccgttc
     6421 agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat ggagaagtca
     6481 ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact catactttat
     6541 ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta atatacggtt
     6601 cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg cgctgctggt
     6661 gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata atttgccaac
     6721 cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct ggcgaaagcg
     6781 tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg gcgaccatgt
     6841 tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactctg cctggacgca
     6901 acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg agcaccgcat
     6961 ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga gcggcgcaat
     7021 gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt gcgcggtcga
     7081 tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc attttgattg
     7141 cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg cccatttccg
     7201 ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct ttggtggtgc
     7261 cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc tgcattgctt
     7321 tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac agccacggcg
     7381 agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt taaaaaagga
     7441 accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc tgccacttcc
     7501 gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc gttttcaaaa
     7561 aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga gtttgcaacc
     7621 ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga ataattcgca
     7681 aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc agtgggcacc
     7741 gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc gtttatggcg
     7801 cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca atacggtgag
     7861 tacagcggaa gtaggtacca gagttgcgca atcgctgggc gtaccgcgcg agcaaattat
     7921 caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac aggcgattgg
     7981 tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga tgatcttttt
     8041 tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca tcgactcgcc
     8101 gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata gcgatcgtgt
     8161 tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag gcgagccacg
     8221 gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg gtattaacca
     8281 gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac gactccgcca
     8341 ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta tccattagtc
     8401 ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc atcgccagta
     8461 actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg tgaatcaaca
     8521 cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg cgaaacagga
     8581 tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg gtccatagca
     8641 cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg tcgaccgcgc
     8701 cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg atttggtctt
     8761 tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca ccacgcatcc
     8821 gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca ccataaatag
     8881 tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc agttgtcggg
     8941 cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc tggctgtcat
     9001 acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga ctgagttttt
     9061 cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc ctcataaaag
     9121 ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga tcaattgttg
     9181 aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt ggctgaagtg
     9241 tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca ggggtatcca
     9301 cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg taccacagaa
     9361 cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag ttgacgtaac
     9421 gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag ttatcgccaa
     9481 cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca cgtgagaagt
     9541 acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta aacgcttctt
     9601 ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga gcgaggttat
     9661 cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc acattaacga
     9721 tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc caccagacgt tctgttcctg
     9781 actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg gcgcgggcaa
     9841 catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc gcgcgttcaa
     9901 gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg ggcagacgcg
     9961 tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct catcagcagt
    10021 cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg ggaaagccag
    10081 gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt ttccgttgca
    10141 aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag atggatcggg
    10201 ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt cccagccagt
    10261 gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg ttgcctgctg
    10321 ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg tcgttggttt
    10381 aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc gatgcatttt
    10441 ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa aatagccata
    10501 ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa aaagctgctc
    10561 tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc tgtgcgttgc
    10621 agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat gcagccaaag
    10681 attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc tcgcttcgcg
    10741 ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact gttcgcggaa
    10801 tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg tttgcgaaac
    10861 gagatcgcgc tcatcatccc agtcaggcat gactcggtgg gttaaatcat aaacgcgctg
    10921 gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt ccagatgccg
    10981 tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct caaaatcggc
    11041 tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt ccgcctcatg
    11101 ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca gcatgcggtg
    11161 gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc agtattccat
    11221 taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc ccagacgact
    11281 gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt gcagcaagga
    11341 catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg gtttgtttaa
    11401 caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt gcggcagcga
    11461 cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag aagccagcga
    11521 ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt ctacaggcaa
    11581 ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt cagtcattac
    11641 cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag actgatgatc
    11701 ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg caaaaaagcg
    11761 cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa cgtcacaccg
    11821 cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga tttcaccgct
    11881 gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc ccgctcgctc
    11941 acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac catcaataac
    12001 gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg ttacgatgat
    12061 ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa cgggagaaac
    12121 cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg cctgtgctgt
    12181 ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat atccccgcga
    12241 tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga caacgaccgg
    12301 ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta cgggggcacg
    12361 ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca ccagaccata
    12421 caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc agattttttc
    12481 gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg ctcaagcaaa
    12541 tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat ttcgtctgcc
    12601 ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt ctgcaactca
    12661 tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt agcttcatcc
    12721 agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg ctgacgctga
    12781 ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt atccatctta
    12841 ttgatgaagt ccatggcgta ggccatacgc gccgcttctt caatttgctc acggctgtac
    12901 tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag atggacattc
    12961 tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg cagatcgtga
    13021 ccatccatca ggatttcacc ttcatcaata tcgtaaaaac gcgtgatcag gctggcaatg
    13081 gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc cggaattttc
    13141 aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt gacattgcgg
    13201 aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt ctcctgctca
    13261 ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa ctgggcgtta
    13321 acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa aacaacggta
    13381 atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata cagaacaaac
    13441 gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga ggctgaaacc
    13501 attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt cgtttccact
    13561 tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc tgcactggtg
    13621 gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg cttcgatact
    13681 acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga cagttgccaa
    13741 ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg cacaacagta
    13801 atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat acgtgacaac
    13861 agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat gtgaccaaac
    13921 aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca gtagctggag
    13981 acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg catccacacc
    14041 agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag gagcgataac
    14101 atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat cagacccgct
    14161 ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga gagatctttg
    14221 tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta cccgttatcc
    14281 actggcacgc caaaccactg ataataccaa cgaggtaaaa tttgctctct gaggctgcta
    14341 atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg agtatcaagc
    14401 cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa tcgccatgcg
    14461 ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat taatggcaac
    14521 gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg cacttgctgc
    14581 cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt tagaagaatg
    14641 ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt atcgttgcta
    14701 ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc ttcgccacgc
    14761 acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat cggctatata
    14821 tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag aataacgcct
    14881 tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc actatcccct
    14941 tcaggccagg ccagtcctgt gtcatagaga atcgctttgc cgtttctggc tatcaccatt
    15001 gccagccctt gcccgacatc aagcatgtac agctgccact cgtcaggtcg aggtttttgc
    15061 cacagcggcc agcacatcag caagcctcca gccacacaca ttactggcaa cgttcgccag
    15121 gcgtttaatc gccataccac cagtaagaac catggggaaa atgatagcca ttgccaacat
    15181 tcaacaatgt tgatccaccc ttccggcaaa ctctttaacc cctgttccag gattaacggc
    15241 ccgctaaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag
    15301 ggaattgcaa atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt
    15361 tgcatgggca taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca
    15421 cgcaataccg gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt
    15481 gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc
    15541 agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt
    15601 aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt
    15661 cccgtcaacc aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag
    15721 tggatccagc gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac
    15781 gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc
    15841 atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata
    15901 accgcattcc acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc
    15961 aggctacaat tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc
    16021 tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca
    16081 cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg
    16141 ccatacagca caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga
    16201 ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg
    16261 ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa
    16321 aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag
    16381 gcgaagagag tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac
    16441 ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgcc cccctccctg
    16501 tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga
    16561 ggcacattca gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct
    16621 tccagttcct gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag
    16681 tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa
    16741 agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg
    16801 tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc
    16861 cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct
    16921 tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga
    16981 ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg
    17041 ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca
    17101 acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga
    17161 aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga
    17221 ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt
    17281 tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag
    17341 cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag
    17401 ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc
    17461 ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca
    17521 gggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt
    17581 ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa
    17641 ccaggccgtc gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga
    17701 ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag
    17761 ctttgcactg tttcagccct agggagatac gacgacgttc ttcgtcgata tccagaacca
    17821 taacttccac tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc
    17881 aatccatttc ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc
    17941 cgtagtcagt caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag
    18001 cgatagctac ccacggatct tcgcccagct gtttcaagcc cagggataca cgggtacgtt
    18061 cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg
    18121 gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca
    18181 gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt
    18241 cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac
    18301 gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct
    18361 ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaaccaggca
    18421 ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa
    18481 caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac
    18541 gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag
    18601 caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct
    18661 cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga
    18721 tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact
    18781 cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc
    18841 cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg
    18901 gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc
    18961 tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc
    19021 cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc
    19081 caaaaggcgc tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg
    19141 cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac
    19201 cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg
    19261 acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac
    19321 ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc
    19381 attggtcgac acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga
    19441 cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga
    19501 gtccagcaga tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc
    19561 accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa
    19621 acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac
    19681 gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc
    19741 gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat
    19801 cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc
    19861 gaccgccatc caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc
    19921 cacgtttctt caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt
    19981 atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag
    20041 ccacgccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca
    20101 tttcagccag cgagtgaccc aacgcgacg
//
LOCUS       DAJVQH010000070        19903 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJVQH010000070 DAJVQH010000000
VERSION     DAJVQH010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.070"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..375
                     /locus_tag="OQM87_002517"
     CDS             <1..375
                     /locus_tag="OQM87_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HCS1508613.1"
                     /translation="GAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMS
                     ERPIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPIRCAFTGALTLYYYFIG
                     FFGILAAIAITLVWQRHTRFFH"
     gene            complement(411..1367)
                     /locus_tag="OQM87_002518"
     CDS             complement(411..1367)
                     /locus_tag="OQM87_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCS1508614.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="OQM87_002519"
     CDS             complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="OQM87_002519"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCS1508615.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2605..3366)
                     /locus_tag="OQM87_002520"
     CDS             complement(2605..3366)
                     /locus_tag="OQM87_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1508616.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3499..3909
                     /locus_tag="OQM87_002521"
     CDS             3499..3909
                     /locus_tag="OQM87_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCS1508617.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3871..4977)
                     /locus_tag="OQM87_002522"
     CDS             complement(3871..4977)
                     /locus_tag="OQM87_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1508618.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4988..6121)
                     /locus_tag="OQM87_002523"
     CDS             complement(4988..6121)
                     /locus_tag="OQM87_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1508619.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6114..7850)
                     /locus_tag="OQM87_002524"
     CDS             complement(6114..7850)
                     /locus_tag="OQM87_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1508620.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7843..8838)
                     /gene="hlyD"
                     /locus_tag="OQM87_002525"
     CDS             complement(7843..8838)
                     /gene="hlyD"
                     /locus_tag="OQM87_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCS1508621.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="OQM87_002526"
     CDS             complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="OQM87_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCS1508622.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9741..10928
                     /gene="rhlE"
                     /locus_tag="OQM87_002527"
                     /pseudo
     CDS             9741..10928
                     /gene="rhlE"
                     /locus_tag="OQM87_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="OQM87_002528"
     CDS             complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="OQM87_002528"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCS1508623.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            11737..13887
                     /gene="dinG"
                     /locus_tag="OQM87_002529"
     CDS             11737..13887
                     /gene="dinG"
                     /locus_tag="OQM87_002529"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCS1508624.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13915..14877
                     /gene="ybiB"
                     /locus_tag="OQM87_002530"
     CDS             13915..14877
                     /gene="ybiB"
                     /locus_tag="OQM87_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCS1508625.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15018..16103
                     /gene="hcxB"
                     /locus_tag="OQM87_002531"
     CDS             15018..16103
                     /gene="hcxB"
                     /locus_tag="OQM87_002531"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCS1508626.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHKETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(16332..16592)
                     /locus_tag="OQM87_002532"
     CDS             complement(16332..16592)
                     /locus_tag="OQM87_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1508627.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(16857..17123)
                     /gene="ybiI"
                     /locus_tag="OQM87_002533"
     CDS             complement(16857..17123)
                     /gene="ybiI"
                     /locus_tag="OQM87_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCS1508628.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17197..17874)
                     /gene="ybiX"
                     /locus_tag="OQM87_002534"
     CDS             complement(17197..17874)
                     /gene="ybiX"
                     /locus_tag="OQM87_002534"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCS1508629.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(17916..19903)
                     /locus_tag="OQM87_002535"
                     /pseudo
     CDS             complement(17916..19903)
                     /locus_tag="OQM87_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4614 a         5230 c         5376 g         4683 t
ORIGIN      
        1 ggcgcgatgt ttttaatcag tatgctggcc ggtttattat ttaatgttga tcctggttct
       61 caccgtttta tcattatgat gacgttgaca gggttggccc tggtaatcat cgtgaatgcg
      121 gcattaatga gtgaacggcc catttgggta ataagttgct taatgattgt gttatggcca
      181 ggcattatct cgcatggacg aaataagctc cttgaactgg caggaaaatg ccatagtgaa
      241 gagttgtgga gtccgattcg ttgcgctttt acaggtgcat taacactcta ttactatttt
      301 atcggcttct ttgggatact tgccgcgata gctataacgc ttgtctggca aaggcatacg
      361 cgtttttttc attagtcgta aatgcagata ctaaagaact taactaaact tcacatcgcc
      421 gcttcatttt tcgcccgtag cttcttcgct tggctttcca gtagcaggta acagatcagc
      481 gccagcagca gcgggataaa gtaatacagg acgcgataag cgagcagggc ggcgataatg
      541 ctccctttcg aggtatgctc cccagccagt agtgcgataa acaccgcttc cagcacaccg
      601 atccccgccg gaatatgcac gatgacgcca gcaatactac taaccagtaa cacgcccagt
      661 acaaagaaat agttcacgct ttgaccaagt aacagccaga taatcgcccc cattaccatc
      721 cagttaacgc tggaaatcag catctgagcg agggcgaatt tccatgaagg cagcaccagc
      781 ttttgccctt tgatggtcat atggcggtgc ttcgcgaaag cgcaaaacca caaataaacc
      841 gcgataatca tcagtaagcc aatgccgaga atacgcagcg tagtttgatc gacataccag
      901 tgatccggca actccaccac gcctgcggta aagataatcc ctgccagtaa aatatagccc
      961 agccagttgg tggtaatact gagcgaggaa atccgcgtaa tagtgctgcc cggtaaccct
     1021 agtcgggagt acaaacgata gcgcatacca atgccgccga cccaggtact gagcgtcagg
     1081 ttgaaggcgt agcagataaa cgacaccagc atcacctggc gcttcgccag tttgtgaccg
     1141 cagtaaaagc gggcgagcag gtcatagcag ccgtaaatca gatagctgac gaccaccagc
     1201 ccgaccgcac taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc
     1261 cagtccactt ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag
     1321 gtgaggatct tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc
     1381 ccgtgttttc agtttctacc cgatcctgcg tttccattgt cggttgtgcg gggggatcaa
     1441 cctgcgtcag acgtggtgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta
     1501 aaaagtggaa cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg
     1561 tttcatccac ctgctgacaa tctgcggcaa taatgccgtt cagattatcg cgcagcgtct
     1621 ggttaaaatg acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg
     1681 gatcgagatt actggaccct actgtcgccc agtgatcgtc catcaatgcc actttgccgt
     1741 ggagcgggcg gcggcggtac tcaaaaacct gaacgccgcc tttaaccaga tagttataca
     1801 gcaagcgcgc accgactctg acaatcggca tatccggttc gccctgaatg atcagtttga
     1861 tccgcacccc gcgccgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga
     1921 agtaggcgtt ggcgataatc acttcccgcc gcgcctgagt gagcattttc agataatggc
     1981 gttcaatatc atcgcgatgt tcttcgttat cgcgccagac cagcaatacc tgcgcttctc
     2041 ccggctggcg gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct
     2101 gtccaggcag gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc
     2161 gtaccgcgta atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat
     2221 tcagcccgcc aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc
     2281 gaaacacatt ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta
     2341 cgccagctgc cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat
     2401 ccagcaagac ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct
     2461 gtttgccgac gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct
     2521 cgccaatcgc cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct
     2581 tattgccttc gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc
     2641 agaaaggtgt cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt
     2701 gacgtagatc ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg
     2761 tccgtgggcg cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc
     2821 tttttgccgc cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc
     2881 attcacccat tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag
     2941 atgtacgcac atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag
     3001 cacgccgcgc ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg
     3061 ataacgcgac agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta
     3121 ggcaaaatcg ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt
     3181 ttccacatgc agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc
     3241 ggcgctgacg gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc
     3301 ggtaaagcct ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc
     3361 gggcataatt ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa
     3421 aaaggtgcgg tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa
     3481 ttcttcagga gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt
     3541 tgctggcaga ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt
     3601 aagactctgg ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg
     3661 atgctggtgt ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa
     3721 gagctgacca cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc
     3781 gtactggaaa acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg
     3841 gtagtgtctt tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta
     3901 cgccgctgac cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat
     3961 atccagactc gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg
     4021 gttaatccac gtcagatttt gcagccacac tggcatgttt tccaccggcg aaacgtaacc
     4081 ggaaaggaga atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt
     4141 cgagcagagt gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat
     4201 cgtaaagtag aacagcgcta gcgatccggc gaaggggatt tgatacgccc agataccaat
     4261 cgccagcaca atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat
     4321 gaagatctgc caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg
     4381 ttcgcgggcg acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat
     4441 cagcgacggc accacaaacc atttgtagtc gagattcggg ttataccagt tgcgtaccac
     4501 cagctcgctg ttgttgggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac
     4561 gatctgttgc aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat
     4621 caactgcaac ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg
     4681 caccagtagt aacgcctttt gtgtgtcgat ggtcgggcgg atctcctgtg ggcttttcag
     4741 cagcagcaca tgtgtaaagg cgctggcgcg ggcaaaacgt tgagtaagtt ccaccgaatg
     4801 ctcgccgtta tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc
     4861 gaacgggaac aggatcacct gaattagcac gggtaaaatc agaatagcgc gggtttgtgg
     4921 ttcgcgcagc aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct
     4981 cttctcccta atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg
     5041 ccgaagcgat caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga
     5101 acaggctttg cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga
     5161 tcaccgcggg catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagaa
     5221 aagcggcgtt cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca
     5281 gcagccccat tcccagggta ctgagtaaaa acaggctgga gataaaaaac agaatcagca
     5341 gcgaaccgcg atacggcacg ccgagaataa acactgacac cagcatacac agcaacatcg
     5401 ccagcatccc gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa
     5461 tctccgtaga gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg
     5521 aggtgagaat cgcgccgatg accgtcatga taatggtcac cgcacccggg ataataaagt
     5581 gctggcttat cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa
     5641 aagtctgccc gttgtcctcc gctcgctgca tttgccagat ctgccagatc ccttcgacat
     5701 acccctgcac aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg
     5761 cggtggcgtt ggcgcgctcc atctgttcgg cgaaatccac gggaataacc accagaccgc
     5821 gaattttccc cgactgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt
     5881 cgatgtaggg cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt
     5941 ccagcaaaat cccgacccgc agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa
     6001 acagcagtag cagtgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct
     6061 gccgcgtctc tttgacgcac agcgcccgca cgcgatgcca ggaaaggatc gggttactca
     6121 ttgctatgct ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga
     6181 tcgggttgct catcgttagc tgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt
     6241 aatttcccac ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg
     6301 gtggtgacca tcaccgtgac gcctttctct accatgctgt tgatgtgcag ccaaaattca
     6361 cggcgggtga gggggtcaac gccggaagtc ggttcgtcga gaaagagaat atccggttca
     6421 tgcatcagcg aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg
     6481 gcgtgtgagg caatactttt caggccaaat gcctcgctca tacgggagat tttttcgttc
     6541 tgcgcccgac cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc
     6601 aggttgccgt agagcgaaaa tttttgcgcc atatagccga gatgctggcg cgctttaccg
     6661 gaactctctt tcagatccat ccccagcacc agcgcctggc cggaagtcgg caccagcaaa
     6721 ccgcacatca tcttaaaggt ggtcgattta cccgcgccgt ttggccccag caaaccaaaa
     6781 atctccccac gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc
     6841 ttggtcagtt ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat
     6901 attgcaccca gcggcgattc tgaggttccg gcaccgccca gcaaatcaat aaacgcatct
     6961 tcaaaacgcg gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata
     7021 tcgtctggtg tggcctcttt tttgaggatc agacgtaccg atttcccctg aatcatgccg
     7081 tcgctgacct gcggcagttt caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga
     7141 ctggtcatca gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttctccctga
     7201 tacagcaact cgccttcgtt catcagtaac acgtcacggc actgctcggc ttcgtcgaga
     7261 tacgaggtac tccagaggat taacatccct tcgcccgcca gctcatgcac catctgccac
     7321 agttcgcgcc gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc
     7381 ggttcgccca ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg
     7441 cccgccaggc gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt
     7501 tgcttacgtg cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc
     7561 accgtgagat cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc
     7621 gcgccgtcgt ttttgatcgg atcaaagcca atcaccgtgg cactgccgct gtcgggtttc
     7681 agtaatcccg ccaacatccg catcagcgtg gttttacctg caccgtccgg ccccaccaac
     7741 cccgtcacat aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg
     7801 cccggaaagc gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc
     7861 gtcaccgaat tgtaccgtca ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac
     7921 cacaatacgc aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg
     7981 ggtaaattca gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg
     8041 accatcggta taaagcagca ctttgcgccc cggctgggcc tggtcaagat tccgttcatc
     8101 aacataagcg cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc
     8161 attgagaacc gtgcctggct ctaccgcgcg cgttaacagc gtgccatcag acggtgctat
     8221 caacgttgag tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag
     8281 gctggctttc gcctgagcga tatcctgttc acggttaccg gaacggtact gacgcaattt
     8341 atcctgtgct gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag
     8401 gtcatttgcc gaaatggtgc ggcttttcca cagtccttgc tggcggttat agaagttctg
     8461 cgcatagtca taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc
     8521 atcgcgatac ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt
     8581 cgcctgcatc agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc
     8641 tttgatagca tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact
     8701 aagattcacc gtacgaatat ccacgttgcc atacagcgtc aggccgttat cttggcggct
     8761 ttgataccac cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagtccgat
     8821 cacgacaggt tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg
     8881 atatgacagg tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag
     8941 ccggtacgta acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac
     9001 gcatgggaat gaaggatcat gcgggtgtca ttagcgtcgc agccggtcca ggcggcaatc
     9061 aggcgtgtca ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct
     9121 gccgtgggag agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg
     9181 gtgagcagct taatcatgtt cctgcaggcg cgaaggatca gttcacggat ggcagcccga
     9241 tcaggctgtg gttgggcgaa caagcgttcg gcttcctcgg catgcggacg gaactgctcg
     9301 ccaataaaat cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag
     9361 tagtaggtga tggcagcgat attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg
     9421 ttcattccat attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt
     9481 tcacccttga ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt
     9541 gattaagatt atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc
     9601 agtcacaggg ataatttatg cgctgcgtca caaaaactgc tacactccgc tccctcatga
     9661 cattgtggtt tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt
     9721 cggggcggtt cggagtagtt atgtctttcg attctttggg tttaagccct gatatcctgc
     9781 gcgccgttgc cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg
     9841 cggtgctgga aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg
     9901 gctttacgct gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc
     9961 gtccggtacg tgcgctcatt cttaccccga cccgtgaact ggcggcacag attggcgaaa
    10021 acgtccgtga ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggcggtgtca
    10081 gcattaaccc acaaatgatg aagttgcgcg gcggcgttga tgtgctggtg gcaacgccgg
    10141 gacgtttgct ggacctggaa catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg
    10201 tcctcgatga agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt
    10261 taacaaaact gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc tctgacgata
    10321 ttaaagccct ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca
    10381 ataccgcgtc tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg
    10441 aattgctgtc gcacatgatt ggtaaaggga actggcagca ggtgctggtg tttacccgta
    10501 ccaaacacgg cgctaaccat ctggctgagc agctcaataa agatggcatc cgtagtgtgg
    10561 cgatccacgg gaataaatcg caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg
    10621 gtgacattcg cgtactggtg gcgaccgata tcgctgcgcg cggtctggat atcgaagagt
    10681 tgccgcacgt cgtcaactat gaactgccaa acgtaccgga agattatgtc caccgtatcg
    10741 ggcgtaccgg tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac
    10801 acaaactgct gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc
    10861 cgggctatga gccggatccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac
    10921 gtggcggcat caggctgatg aacaaacgca aaactgcctg atgcgctacg cttatcaggc
    10981 ctacgtggac tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta
    11041 cgccgcatcc ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat
    11101 ttatctgttt taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc
    11161 gcaactccat taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc
    11221 agtaagcatc gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagca
    11281 cacgcagttc tgcatgctgt tcgaatttag cgcgaagtgc tttacgcatc acctgttctt
    11341 taaccgactc ccagttttta cgaagtggct tggaacgatt acgccccatg cgagcggcga
    11401 ccatggggga agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg
    11461 cctgaaaata gtgttcggag gtgggccagg tattcccgtc aaccttgatg ggcctggcgg
    11521 caaagttaga aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt
    11581 gcatgacgtg ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt
    11641 attgcgagcc gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac
    11701 aatattggct gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa
    11761 gcgcaaattg ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt
    11821 gcgccgcagc ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg
    11881 catctggcga ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc
    11941 atcgccattg cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg
    12001 caggatcaga tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa
    12061 ttcactgccg ctttcgggcg tgggcgctac gtttgtccgc gtaatctgac ggcgctcgcc
    12121 agcactgaac ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat
    12181 aatcaggaag agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg
    12241 gatggtctgc gcgatcatac ggatatcgct atagatgatg atctctggcg tcgtttgagt
    12301 accgacaaag ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt
    12361 gttactcgtc gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg
    12421 gcggcgatgg aaagcgaagc cgtactgcct gacccgaaaa atctgctgct ggtgctggac
    12481 gaaggtcatc acctgccaga tgtggcccgg gatgcgctgg agatgagcgc tgaaatcacc
    12541 gcgccctggt atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag
    12601 cagtttcgcc cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt
    12661 gaagagttgt atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc
    12721 gggcaggagg cagagcaccg ttttgcgatg ggcgaactgc ctgatgaatt gctggagatc
    12781 tgccagcggc tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac
    12841 gatttaagtg agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttacag
    12901 atgaaccgcg cgctggggat gtttgaggtg caaagcaaac tctggcggct ggcgtctctg
    12961 gcgcaatctt ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag
    13021 ctacacctct ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg
    13081 tggcgcagta ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaatagtttt
    13141 tcgcgtttgc aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg
    13201 gattccccct ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgctttgag
    13261 ccttccatcg acaacgaaga gcaacacatt gccgaaatgg cggccttttt ccgtgaacag
    13321 gtggagagca aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag
    13381 cgctttctcg actatgtgac ggatttacgc ctgatgttgc tggtgcaggg cgatcagccg
    13441 cgttatcgct tagttgaact gcaccgcaaa cgcgttgcca acggtgagcg tagcgtgctg
    13501 gtgggcttac agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg
    13561 catatccaca aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa
    13621 tggctgaaaa gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt
    13681 aacctgattc agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt
    13741 atctacgaca aacgcttgct gacaaaaaat tatggcaagc gactactgga tgcattaccg
    13801 gtatttccga tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg
    13861 aaatccccac gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac
    13921 tatcgcaaaa taattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg
    13981 gatactgcgc gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg
    14041 ggcggcgtat tgattgcgct gcgtatcaaa ggggaagggg aagcagagat gctcggcttt
    14101 tacgaagcca tgcaaaatca caccatcaag ctgacgccgc ctgcaggcaa gccgatgccg
    14161 attgtcatcc ccagttacaa cggcgcgcgt aaacaggcta acctgacgcc attattggcg
    14221 attctgctgc ataaactcgg ttttcctgtg gtggttcacg gagttagcga agatccaact
    14281 cgcgtgctga cagaaaccat tttcgaactg atgggtatta caccaacgtt gcacggcggg
    14341 caggcgcagg cgaagctcga cgaacatcaa ccggtgttta tgcctgtcgg cgcgttttgc
    14401 ccgccgctgg aaaaacaact ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat
    14461 accctggcga aactggcgac gccatttgcc gaaggtgagg cactgtgtct ttccagcgtt
    14521 tcgcatccgg aatacattgg acgcgtcgcg aagttcttta gcgatatcgg cgggtgggcg
    14581 ttactaatgc atggtactga aggcgaagtg tatgctaatc cgcagcgctg cccgcagatc
    14641 aatctcattg accgtgaagg gatgcgggtg ctgtatgaaa aacaggaccc tgctggtagc
    14701 gagttactgc cacaagcaaa agatccggaa accacggcgc agtggactga gcgttgcctg
    14761 gctggcagcg aagcgattcc cgaatcgctg aaaatccaga tggcttgctg cctggtggct
    14821 acgggtgaag cggcaactat cagcgatggc ctggcgcgcg ttaatcaggc attttaattt
    14881 ttatctcagc cggatgagcc acgtcatccg gcttttaccc caccgcaata tgaaattcct
    14941 gcatctttat tgaccttccc gcgcccggcg tgcagcataa aaaaacaaca aacacataac
    15001 ataaacagga gttaaccatg gaaagtggtc atcgctttga tgctcagacg ctgcacagtt
    15061 ttattcaggc tgtatttcgt cagatgggta gcgaggaaca agaagcgaaa ttagttgccg
    15121 atcatttaat cgcggcaaac ctggcagggc atgattctca tggtattggc atgatcccaa
    15181 gctatgtgcg ttcctggagt caggggcatc tgcaaattaa ccatcatgcc aaaatcgtta
    15241 aagaggcggg ggcggcagtc acgctcgatg gcgatcgcgc attcggtcag gtcgcggcac
    15301 acgaagcgat ggcgctgggg attgagaaag cgcatcagca cggtattgct gccgtggcgc
    15361 tacataactc gcatcatatc ggccgtatcg gttactgggc ggagcagtgt tcagcggcgg
    15421 ggtttgtctc tatccacttt gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg
    15481 gtcgcgacag ccgctttggc accaatccct tctgtgtggt tttccctcgt aaagataatt
    15541 tcccgctgtt gcttgattac gccaccagcg ccattgcatt tggcaaaacc cgcgtcgcct
    15601 ggcataaagg cgtccccgtg ccgccaggtt gcctgattga cgttaacggc gtgccgacga
    15661 ccaatccggc ggtaatgcag gagtcgccgt tggggtcgct gttgaccttt gccgaacata
    15721 aaggctacgc ccttgcagcg atgtgtgaaa ttcttggcgg ggcgctttct ggcggtaaaa
    15781 cgacgcataa ggaaacgttg caaaccagcc ccgatgccat tcttaactgc atgaccacta
    15841 tcatcatcaa cccggaactg ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg
    15901 ccgagtgggt gaaagcctcg ccgcatgatg acgataagcc gattttgcta ccgggcgagt
    15961 gggaagtgaa cacgcgtcgc gaacggcagg agcaggggat tccattggat gcgggaagct
    16021 ggcaggccat ttgtgatgcg gcgcggcaga ttggtatgtc ggaagagacg ttgcaggctt
    16081 tctgtcagca gttagccagc taaaaaaaag cccgtccagt ggcggacggg caaacaaggg
    16141 taacatagga tcaatgaggg ttagagcata tgcgtctgtc ggcaaacaga cagggaaata
    16201 cttgtgctgg acgtagcgta aacgcctgat ccgtccaacg gttcggattt tgtaggcctg
    16261 ataagacgcg gtagcgtcgc atcaggcatc tgttgccgga tgcggcataa acgccttatc
    16321 cgtcctacga attacttata aattaccgca gtaccgctta atttattgtt gttggtggcg
    16381 gaagtgatgc tgtatccact ggcaccggct gccgccgctt tctcagccag tttcgcttcc
    16441 agggcatcga gggtggatgc gccatcggca gaaaccacgc cgattttatt catgttctgt
    16501 gcctgggatg ccgttaccgg ttccgccgcg aacacgccaa atgacagagt tgaaagagcc
    16561 atagcagcaa caacagtatt gatagttttc ataattaatc tctcgcaggt gatttttgta
    16621 taagtgacgt tgtttcgtcg atgtgatgag tatcacgttt ttttacgaga gataaaatcg
    16681 aagagaattg accgccttgt tcaaataaat tgattgatat ctaacatact gataaaccat
    16741 ttatttgttg tacttatgga cttgttttat gccgcgtggc agtcacagca gcgttaaagg
    16801 cacggaatgc cacgcaaaat acaaattata ctcgctatgc atctcgccag ttgtagttaa
    16861 cgtaactggc tgtctttcga acctctgcga ttatatcctg tatacgctgg tttttgtaaa
    16921 tctttctcct gctgacaatg aatacataag cgcacgccag gaatggcttc ccgacgggct
    16981 tgcgggatgg gggcaccgca ctcttcacat tcatacaggc tttcgccacg cggaatttca
    17041 ccccgagcgc gggcaatcgc atcttcaatt gtactgttga tctgttcgtt gacggcgtca
    17101 tcgttagccc aagcggatgc catatcgtcc tccccatatc aatgcttgta cagttaagtg
    17161 tagctaatat agggacgaac ctgggcagtt caagcgtcag atctccgacc attcccgcag
    17221 cagattatga taaagattga gcagcgacag gatctcttca ttttcaccat agcggctttt
    17281 cagcgactga atatttttgt ccagttcaaa cagcatggcg cgctttttat catcgcggat
    17341 catcgactgg atccacataa atgatgccac tcgtacgccg cgggttacgg gtgtcacgca
    17401 atgcaggctg ctggagggat acaacaccag atcacctgcc gggagtttta cccgatgttg
    17461 tccaaaggtg tcattaacga ccagttcgcc gccgtcgtag ctttgtggat cgcttaaaaa
    17521 cagcgtggca gaaaggtcag tgcgcatcca gccgttttgt ggatggctgc gtactgcgcc
    17581 atccacatgg aaaccatagg tttcattgtt ctgatagcga ttaaacagcg gcgtggaaag
    17641 ggtacgcggc aaggcagcgg caaagaacaa tgcatgttgg ttaaccgcgt tcagcacctc
    17701 attttgcagt gcggcgtata acgcgctgcg ggtgtctact tgctgattgt tcttaacttg
    17761 tgcaccttgt gctccggtgg tgacgcgtcc atccacccat tcggcttgtt ccagttgttc
    17821 gcgaaaatgg gcgacgtcct gtggcgataa cacgccggga atgtggtaca tcatacaagt
    17881 tctccaaaaa gtggggcctg cgccccacat ctgaatcaga aatgcatatt ggctgtgagc
    17941 aagaaggttc ttggctcgcc cgggtgataa cggtagccgc tcttgttgat tgaggcgacg
    18001 taatcggtat caaacaggtt gtaaacgttt agctggaagt cgagattgcg attaacccga
    18061 taccccagtt tggcatctgc gacccagtaa ccttctgtaa atgctggcgt tcccaccgcg
    18121 ccgtctgaac ctttatgtat actgccgata tagcgtgcgc ccgcgccaac agagatatca
    18181 tcggttgcct gatattggct ccataaggtg aaggcgtgct ccggggtata cggcagcgat
    18241 gaggaaccat cctgggcaac atctttgccg tttttgatgg ttgctttttt ctgggtatag
    18301 ccgccaatca cctgccacgc gggagtgata ttccctgcca cggatatctc atagccttcg
    18361 acgcgtttct taccgtattg cgagtaagtc ccgtcatcat tttgctcaac ttcattttcg
    18421 atatcagtac ggaacagcgc ggcggtgagc aacaggcgtt tatccagaac ctgccatttg
    18481 gtgccaatct cgctggtgtt ggctttttgc ggtttaaaat cggtgcggtt ggcactgtta
    18541 ccactgccag actgcgcaag ggcgaagttg ttgccgcccg gaggctgctg ggaaacggca
    18601 tagttaatat agacattgcc gttttccgtc aggtgataca gcgccccggc tttccagttc
    18661 accagattgc ccgacttggc ggtgtcgacg gtggtgactg gagaaccttt tgccacacca
    18721 gctgggcagg tgatggcacc gcgtccgctg ccgccgcagg cggtggcact gtcatattca
    18781 gtatgataat tatccagacg gatcccgccg ttcagctcaa aatcacgggt gatttgcagc
    18841 gtatcgaagg cgtaaattgc gaaggtatcc gtctgaccat tggcgtttgc gccgttgcgc
    18901 gtcaggccgc cgggatgaat tctgctgtca ggatgataaa tatttaccgc cggtaacgtc
    18961 accggattaa cgccatagtt agtctgcgtc tcacgggtaa attccacgcc ggtactgaca
    19021 tcatgaccga tagaaccggt atagaacgtc gaggtcaggt tggtctggtt ggtgagaatt
    19081 ttattactca catctttggt attcgccgtg cgtgactagg tccagctatt cacatcgctg
    19141 gtgggctgag taatattcga cgccccgccc ataatcgccg tcatcaggta atcctgcttt
    19201 acgcgcgacc aacgggtagt attgcgaatg gtggtgttat cgttgatgtc gtgctcaaaa
    19261 cgcatggtgg cggtgtcggt ggtcgaatcg tcgtaatcgg aatccgtgcc gtaaaagtta
    19321 tgagtatcaa cttttccgga atgattcagg gccgccgctc ccgcagatgg ggcagaatag
    19381 cccggcaaac cgatggtcgg aatgccgccg tctggcgtgt tgtgctgagt gacatgcaga
    19441 taattaagat acaaacgatt cgctgtacca aggccaaaag agacagaagg ggcgacgccg
    19501 taacgctcat ttttgacttt gtcgcgtccg gcatcgtggg ttttttcgcc catcacattc
    19561 aggcgcaccg cggtagtatc accaatgacc tgattgacgt ccagcgtgcc gcggcggaac
    19621 caggcgctgc caatactggc ggaggcgtca atgccggaat cattgcgcgg ctgcttgctg
    19681 atcatattga tcgagcctgt cggtgcgctg cgcccgtagt cggtgccgga cggcccttta
    19741 atcacttcga cctgctcggt attgaaggtg tcgcgcgaga cgctgccgat atcgcgaata
    19801 ccatcaatat aaatactgtt agaggtatcg gcaccacgca tataaatggc gtcgccagtg
    19861 gtggagttac cgttctcacc cgcaaaaaac gcacccacgc cgg
//
LOCUS       DAJVQH010000071        19826 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJVQH010000071 DAJVQH010000000
VERSION     DAJVQH010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.071"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..656)
                     /locus_tag="OQM87_002536"
     CDS             complement(<1..656)
                     /locus_tag="OQM87_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1508630.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSY
                     MWFETGDNGNEYFKWRHHLTGGRVKDLMNLKWDALYVLVKALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLVVTGDIF
                     TNRFLINSSTGMWIHMRDQNVIMGRNAVSNDGAQALLRQDHTDRKFVIGGLGNRQFGI
                     "
     gene            1041..1229
                     /locus_tag="OQM87_002537"
     CDS             1041..1229
                     /locus_tag="OQM87_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1508631.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            1180..1875
                     /locus_tag="OQM87_002538"
     CDS             1180..1875
                     /locus_tag="OQM87_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HCS1508632.1"
                     /translation="MGKEKKSRILTKPCVIEYEGQIVGYGSKELRVETISCWLARTII
                     QTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNRGYMELNRM
                     WLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQASNFDFIGSH
                     ESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYIRFLNKRARKR
                     LNTKLFKVQPYPK"
     gene            complement(1987..2334)
                     /locus_tag="OQM87_002539"
                     /pseudo
     CDS             complement(1987..2334)
                     /locus_tag="OQM87_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2303..3960)
                     /gene="dpiB"
                     /locus_tag="OQM87_002540"
                     /pseudo
     CDS             complement(2303..3960)
                     /gene="dpiB"
                     /locus_tag="OQM87_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            4341..5399
                     /gene="citC"
                     /locus_tag="OQM87_002541"
     CDS             4341..5399
                     /gene="citC"
                     /locus_tag="OQM87_002541"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCS1508633.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5414..5710
                     /gene="citD"
                     /locus_tag="OQM87_002542"
     CDS             5414..5710
                     /gene="citD"
                     /locus_tag="OQM87_002542"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCS1508634.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5707..6615
                     /gene="citE"
                     /locus_tag="OQM87_002543"
     CDS             5707..6615
                     /gene="citE"
                     /locus_tag="OQM87_002543"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCS1508635.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACVREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6626..8158
                     /gene="citF"
                     /locus_tag="OQM87_002544"
     CDS             6626..8158
                     /gene="citF"
                     /locus_tag="OQM87_002544"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCS1508636.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8162..8713
                     /gene="citX"
                     /locus_tag="OQM87_002545"
     CDS             8162..8713
                     /gene="citX"
                     /locus_tag="OQM87_002545"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCS1508637.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8688..9566
                     /gene="citG"
                     /locus_tag="OQM87_002546"
     CDS             8688..9566
                     /gene="citG"
                     /locus_tag="OQM87_002546"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCS1508638.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9617..11080
                     /gene="citT"
                     /locus_tag="OQM87_002547"
     CDS             9617..11080
                     /gene="citT"
                     /locus_tag="OQM87_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCS1508639.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11194..12000
                     /gene="rna"
                     /locus_tag="OQM87_002548"
     CDS             11194..12000
                     /gene="rna"
                     /locus_tag="OQM87_002548"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCS1508640.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12229..12639
                     /gene="rnk"
                     /locus_tag="OQM87_002549"
     CDS             12229..12639
                     /gene="rnk"
                     /locus_tag="OQM87_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCS1508641.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(12870..14107)
                     /locus_tag="OQM87_002550"
                     /pseudo
     CDS             complement(12870..14107)
                     /locus_tag="OQM87_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            14328..14756
                     /gene="uspG"
                     /locus_tag="OQM87_002551"
     CDS             14328..14756
                     /gene="uspG"
                     /locus_tag="OQM87_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCS1508642.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14877..16442)
                     /gene="ahpF"
                     /locus_tag="OQM87_002552"
     CDS             complement(14877..16442)
                     /gene="ahpF"
                     /locus_tag="OQM87_002552"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCS1508643.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16687..17250)
                     /gene="ahpC"
                     /locus_tag="OQM87_002553"
     CDS             complement(16687..17250)
                     /gene="ahpC"
                     /locus_tag="OQM87_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCS1508644.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17622..18368
                     /gene="dsbG"
                     /locus_tag="OQM87_002554"
     CDS             17622..18368
                     /gene="dsbG"
                     /locus_tag="OQM87_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCS1508645.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKHGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18578..19480
                     /locus_tag="OQM87_002555"
     CDS             18578..19480
                     /locus_tag="OQM87_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1508646.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            19627..19826
                     /locus_tag="OQM87_002556"
                     /pseudo
     CDS             19627..19826
                     /locus_tag="OQM87_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         4995 a         5100 c         4740 g         4991 t
ORIGIN      
        1 tagatgccaa attgcctatt ccccagccca ccaattacga atttacggtc ggtatggtcc
       61 tggcgaagca atgcctgcgc accatcgttg gataccgcat tacggcccat aataacgttc
      121 tggtcacgca tatgaatcca catgcctgta ctgctgttaa ttaaaaaacg gtttgtaaat
      181 atatctcctg taacaaccag accatgcccc atgcttatcc ggccagttct gagattaagc
      241 gtaaaggggc gtagtggccc tatattacca ttttcccctt cattctctcg tgtagggatg
      301 atatgcaggc attcttcaga acgacgaaaa atggcaccaa aagatgaatt aaatattctc
      361 agtgcattga ccgtcgatat ttttacttca ctgctgaaaa gggctttaac aagaacatac
      421 agagcatccc atttaagatt catcaggtct ttaacccggc cgccagtgag gtggtgtctc
      481 catttgaaat actcattgcc gttgtcgcct gtttcaaacc acatgtatga atcagtatcg
      541 ctgtcggcat catttttaaa tccaatcttt gcccagtcag tattccgaat ccaggcaagg
      601 attgagtcgt tttcaaaagt aagtccaccg gacaacgtat cgccattttt ttgcacggcg
      661 ttaccagcct tgtttaccgt ttcctgtaaa ccgaggggcc gtttctggcc tgcatggcat
      721 gatttccgct tttaaacggg gaattcagcg tgctgattgg ctatgtaagg gtatcaacaa
      781 atgaccagaa tacagacctg caacgaaacg ctcttgtttg tgcaggatgt gaataaatat
      841 ttgaagataa attaagcgga acaaggacag accgaccggg attaaaacgt gctttaaagc
      901 gccttcaaaa aggaacgcat atagaaaacg acgatcgaat caattaaatc gatctgtatt
      961 accgatcgat tatgtcccga taaccacacc cgactgatga gattttttaa gataacggcg
     1021 aattgatgca aggaggtgaa atgaaatcaa ttcgctgtaa gaactgcaac aaactgttat
     1081 ttaaagcgga ttcctttgat cacattgaaa tcaggtgtcc gcgttgcaaa cgtcacatca
     1141 taatgctgaa tgcctgcgag catcccacgg agaaacattg tgggaaaaga gaagaaatca
     1201 cgcattctga cgaaaccgtg cgttattgag tatgaaggcc agattgttgg ctatggttca
     1261 aaggagctgc gcgttgaaac catatcctgc tggctggccc gcacaatcat tcagacaaag
     1321 cactattccc gccgttttgt gaataactct taccttcacc tgggagtatt cagcgggcgt
     1381 gatctggttg gcgttctcca gtggggatat gcccttaacc ccaactcagg tcgtcgtgtc
     1441 gtgcttgaaa cggataaccg gggctatatg gagttgaatc gcatgtggct acacgacgac
     1501 atgccccgca actctgaatc acgggccatc agctacgcgc tgaaagttat cagattactg
     1561 tatccgtcag tggagtgggt tcagtccttt gctgacgaac gctgcggact cgcaggcgtt
     1621 gtgtatcagg cgtcgaattt tgattttatt ggcagccatg aaagcacgtt ctacgagctg
     1681 gatggtgagt ggtatcacga gataacgatg aacgcgatta agcgcggagg gcaacgaggc
     1741 gagtatttgc gagctaataa ggagcgtgcc gtggtgcaca aatttaatca gtatcgctac
     1801 atcagattcc tgaacaagcg agcaaggaag cggctaaata ccaaactatt caaggttcag
     1861 ccatacccta agtgatcccc atgtaatgaa taaaaagcgg taattaatac tgctgtttca
     1921 tttgaagcgc gaaagctaaa gttttcgcat ttatcgtgaa acgctttcgc gtttttcgtg
     1981 cgccgcttca cccagccgtt cataggcaat tggcttaatg agataatcaa atacaccaca
     2041 acgtacggct tcagacaccg tttccatatc actggctgca gtggtaaaca ccacgtcgcc
     2101 gggataatgc gcctgcacca gttcatgcag taaattaatc cctctaccgt caggaagata
     2161 gttatcgagc aagattagcc ccggcttaaa acgctcgatc atcattcggg cctgcgccag
     2221 gtttcccgcc agtaatatct gactgaatcc gggaatgtga cgaatatatt ccgcatgcat
     2281 ctctgccagc ggcgtttcgt cctcaacgat caatagggtt aatggagctg tcattaggtt
     2341 tcactttcgg aatatagatt gaaaataagg taccgcaggg atcattatct tcgagagtga
     2401 taacaccacc gcagcgcgtt acgtagctgg caatcaagta caacccaatg ccatgttcac
     2461 cgggctcgtc agcacgcgta ctgacccctg ctcaaatatt ttgtctcgta gagactctgg
     2521 aacgccgcag ccctgatcgg cgacttcaat caccacatca tcgccttcat cgctgaggaa
     2581 taattcaacg ctcttgtttc cttcatcgct acgcaggctg gcttcgaagg cgttatcaag
     2641 taaattgcca acaatggcgg caaactcggt gctatccagt cctggcggca gttgcgaaag
     2701 ctggctaccg gggacgatga tcatttttag ccccagttcg cgggcgcgct gcactttacc
     2761 aaaaagcagc cccgccacct ggcgatcggc aaacgcctcg cgcaggctgt caataagctg
     2821 ttgctgggcc tgagactccc cctgcaccat cgccagcacg cgatcatact ctttcatctg
     2881 caacagacca ttgagagtcg acatccaatt gagatgctcg tgtcgcaatg tacgaaggct
     2941 ctcaacgtat tgttttattt gcgtcagttg cgcattgagg gtggaaattt cgtctttact
     3001 gcgaaagctg ataatggctc ccagcaaatc atcacctgaa cgaatagctt cccgattggc
     3061 aataacgctc agaccgttaa agttagccac cacatcctga cgtttttcat caatctgttc
     3121 ggtaaagaaa tcggcagacc tgaccacttc agcaatgggt ttacccaacc attgccgtcc
     3181 aggggaactc agccccagca tctttcttgc gttacgattg atggcggtaa tgtaaccgtg
     3241 cggatccacc gcaatcagcc cttcataaac cgaactaaac agcgcctctt gctgacggac
     3301 cacccgtgcg atttgctttg gctccatgcc catcatctgc cgacggatat gcgcggccag
     3361 gaaccacgac agcaacatca gaatccctaa cagcacgaca aacacacctg ccatcggtaa
     3421 taaaaactca gcccgccagc tatcgatttt actcaccagg tagccaatcg acaccacgcc
     3481 gatgactttt ccatcgtcat caaagattgg cgttttggcg cgcatcgcca tacccattga
     3541 ccctttcccg gtaatgaagt agctctcccc tttctccagc gcgccctgtt tggtgaactg
     3601 cataggataa ccaattttct ccggattagg atggtaaagg cggatcgagt gccggtcgcc
     3661 aatcaccaca taatcaaaat cggtatctct ttgtaatttg ttagcgatgg tcgccagccg
     3721 tttgtagtca cgcgttttca ccgcagagat gacactgtca ttggaggcaa taattttcgc
     3781 ctgattcatt gccatgtcgc gtacatgaag cgttaaatag tcctcaaaac tggccgtaaa
     3841 atattgcgcc agggctgcaa tgacaaatat tgagaacacc agaatcagca aaaagatacg
     3901 cagcggaaat gtcagtcttt ggaaaaatgc aaactgttta ttctcgttaa gctgcaacat
     3961 tgttttcctt gcgggttgtc ccgagcctct ttgacggatg taattaaaat gttgaatcaa
     4021 aatacataga tattaattat gatcgtggca aattttattc attaaatcaa ttaaatcact
     4081 taaacctgcg agtcatttcg taattacatt aattaaaaaa cttaaaacca ttaaacaaat
     4141 aaaaaaacca ctaactctat gtgaaataaa tcaaaatttc acgccgaaat actccttagg
     4201 atgtatagcg aaaggagaaa aagatatacc tcgatcaccc ccctttctcc caagtgaaaa
     4261 taaaaggtta tcagtttgca acattgaaca acattcgttg caaatcgata acaacatgca
     4321 ccttcaggat actatttatt atgttcgtca atgatatttt cacccgcgta aaacgttcag
     4381 aaaataaaaa aatggcggaa atcgcccaat tcctgcatga aaatgatttg agcgttgaca
     4441 ccacagtcga agtatttatt accgtaaccc gcgatgaaaa gcttatcgcg tgcggtggaa
     4501 ttgccggaaa tattattaaa tgcgttgcta tcagtgaatc cgttcgcggt gaaggactgg
     4561 cgctgacatt agccactgaa ctgataaacc tcgcctatga gcggcacagc acgcatctgt
     4621 ttatttatac caaaaccgaa tacgaggcgc tgttccgcca gtgcggtttt tccacgctga
     4681 cctgcgtacc cggcgtgatg gtgctgatgg aaaacagcgc cacgcgactg aaacgctatg
     4741 ccgaatcgct gaaaaaattg cgtcatccag ggaacaagat tggctgcatt gtgatgaacg
     4801 ccaatccctt tacgaatggt caccgttatc tgattcaaca agctgcggca cagtgcgact
     4861 ggtttcatct gtttttagtc aaagaagatt cttcacgctt cccctatgaa gaccggctgg
     4921 atctggtgtt aaaaggcacc gccgatattc cacgcctgac tgtgcatcgc ggctcagaat
     4981 acatcatctc ccgcgctacg ttcccttgct acttcattaa agaacagagc gtcattaacc
     5041 attgttacac cgaaattgat ctgaagattt tccgtcagta cctcgctccc gcgcttggtg
     5101 taactcaccg ctttgtcggt actgaaccct tttgtcgcgt taccgcccag tacaaccagg
     5161 atatgcgcta ctggctggaa acgccgacta tctccgcagc gcccatcgaa ctggttgaaa
     5221 ttgagcggct gcgttaccag gagatgccga tatccgcttc ccgggtacgt caactgctgg
     5281 cgaaaaacga tctcacggct atcgcgccgc tggtccctgc agtcacgctg cattatttgc
     5341 agaacctgct tgaacactcc cgccaggacg cggcagctcg tcaaaagacc cccgcatgag
     5401 aaacaggtga aaaatgaaaa taaaccagcc cgccgttgca ggcacccttg agtctgggga
     5461 tgtgatgata cgcatcgccc cactcgatac gcaggatatc gacctgcaaa tcaatagcag
     5521 cgttgagaaa cagtttggcg atgcaattcg caccaccatt ctggacgttc tcgcccgcta
     5581 caacgtgcgc ggcgtacagc tgaatgtcga tgacaaaggc gcactggact gcattttacg
     5641 tgcacgactg gaagccctgc tggcacgcgc cagcggtatc ccggctctgc catgggagga
     5701 ttgccaatga tttccgcttc gctgcaacaa cgtaaaactc gcacccgccg cagcatgttg
     5761 tttgtgcctg gtgccaatgc cgcgatggtc agcaactcct tcatctaccc ggctgatgcc
     5821 ctgatgtttg acctcgaaga ctccgtggca ttacgtgaaa aagacaccgc ccgccgcatg
     5881 gtttatcacg cgctgcaaca tccgctgtat cgcgatattg aaaccattgt gcgtgtcaac
     5941 gcgctggatt ccgaatgggg tgttaacgac ctggaagccg tcgttcgcgg tggtgcggac
     6001 gttgtgcgtc tgccgaaaac cgataccgct caggatgttc tggatattga aaaagagatc
     6061 ctgcgtatag aaaaagcctg tgttcgtgaa cccggcagca ccggcctgct ggcggcgatt
     6121 gaatctccgc tgggcattac ccgcgcagtg gaaatcgctc acgcttccga gcgtttgatc
     6181 ggtatcgccc tcggtgcaga agactatgtg cgcaacctgc gtacagaacg ctccccggaa
     6241 ggaactgaac tgctgttcgc acgctgttcc attttgcagg ccgcgcgctc tgcgggtatt
     6301 caggcgttcg ataccgtcta ttccgacgct aacaacgaag ccggattcct gcaagaagcc
     6361 gcccacatca aacagctggg ctttgacggc aaatcgctga tcaacccgcg tcagattgat
     6421 ctgctgcaca acctctacgc accgacccag aaagaagtgg atcacgcccg ccgcgtcgta
     6481 gaagccgctg aagccgccgc tcgcgaaggc ctcggcgtgg tttccctgaa cggtaagatg
     6541 gtggacggtc cggttatcga tcgcgcccgt ctggtgctct cccgtgcaga actttccggc
     6601 atccgcgaag aataaggcaa tcaaaatgac gcagaaaatt gaacaatctc aacgacaaga
     6661 acgggtagcg gcctggaatc gtcgcactga atgcgatctt gccgctttcc agaactcacc
     6721 aaagcaaacc taccaggctg aaaaagcgcg cgatcgcaaa ctgtgcgcca acctggaaga
     6781 agcgattcgt cgctcaggtt tacaggacgg catgacggtt tccttccatc acgctttccg
     6841 tggcggtgac ctgaccgtca atatggtgat ggacgtcatc gcgaagatgg gctttaaaaa
     6901 cctgaccctg gcgtccagct ccctgagtga ttgccatgcg ccgctggtag aacacattcg
     6961 ccagggcgtg gttacccgca tttatacctc tggcctacgc ggtccactgg cggaagagat
     7021 ctcccgtggt ctgctggcgg aaccggtgca gatccactct cacggcggtc gtgtgcatct
     7081 ggtacagagc ggcgaactga atatcgacgt ggctttcctc ggcgtcccgt cctgtgatga
     7141 attcggtaat gccaacggct acaccggtaa agcctgctgc ggctccctcg gctatgcaat
     7201 ggttgatgcc gacaacgcaa aacaggtcgt gatgcttacc gaagaactgc tgccttatcc
     7261 gcataatccg gcaagcattg agcaagatca ggttgatttg atcgtcaaag ttgatcgcgt
     7321 tggcgatgct gcaaaaatcg gcgctggtac tacccgtatg accactaacc cgcgcgaact
     7381 gcttattgcc cgtagcgctg cggatgtgat tgtcaactct ggctacttca aagaaggttt
     7441 ctccatgcaa accggcaccg gcggcgcttc gctggcggta acccgtttcc tggaagacaa
     7501 aatgcgtagc cgcgatattc gcgccgactt cgccctcggc ggtattaccg cgacgatggt
     7561 tgatctgcac gaaaaaggtc tgatccgcaa actgctggat gtgcagagct ttgacagcca
     7621 tgctgcgcaa tcgctggccc gtaaccccaa tcacatcgaa atcagcgcca accagtacgc
     7681 taactggggt tcgaaaggcg catcggttga tcgtctcgac gtggtggtac tgagcgcgct
     7741 ggaaattgac aggcagttca acgttaacgt gctgaccggt tctgacggcg tactgcgtgg
     7801 cgcttccggt ggtcactgcg ataccgcgat tgcctctgcg ctttccatca tcgtcgcgcc
     7861 actggtacgc ggtcgtattc cgactctggt ggataacgtg ctgacctgca tcaccccggg
     7921 ctccagtgtc gatattctgg tcacagacca cggtatcgca gttaacccgg cacgtccgga
     7981 actagcagaa cgtctgcagg aagcgggcat taaagtggtt tccattgagt ggctgcgcga
     8041 acgtgcgcgt ctgctgaccg gtgaaccaca gccgattgaa ttcaccgacc gcgtcgttgc
     8101 cgttgtgcgt taccgcgatg gctcggtgat cgatgttgtg catcaggtga aggaataagc
     8161 catgcacctg cttcctgaac tcgccagcca ccatgcggtg tcaattcccg agctgctcgt
     8221 cagccgggat gaaaggcaag cacggcaaca cgcctggctc aagcaccatc ctgttccact
     8281 ggtctccttt accgtggttg cgcctgggcc gattaaagac agcgaggtca cacgccgaat
     8341 ttttaatcat ggcgtgacag ccttgcgtgc cttagccaca aagcagggct ggcaaattca
     8401 ggagcaggct gcactggttt ccgccagcgg gccggagggc atgttgagca ttgccgcccc
     8461 ggctcgcgat ctcaagctcg ccaccattga gcttgaacat agtcatcctc tcgggcggtt
     8521 atgggatatc gatgtcctga cgcccgaagg cgaaattctc tcccgccgcg actattcact
     8581 gccgcctcgc cgctgcctgt tgtgcgagca aagcgcagcc gtctgcgcgc gtggaaaaac
     8641 ccatcaactg accgatttac tcaaccgcat ggaggcactg ctgaacgatg tcgatgcctg
     8701 caacgtcaac taaaaccaca aagcttgcga cgtcattaat cgatgagtac gccctgctgg
     8761 gctggcgcgc catgctgact gaagtcaatc tgtcaccgaa accaggcctc gtggatcgca
     8821 ttaactgcgg tgcgcacaaa gatatggcgc tggaagattt ccaccgcagc gcgctggcga
     8881 ttcagggctg gttaccccgt ttcattgaat ttggtgcctg tagtgcagaa atggcaccgg
     8941 aagcggtact ccacggatta cgcccaattg gtatggcttg cgaaggtgat atgttccgcg
     9001 ccactgcggg cgtaaacacg cataaaggca gcattttttc tttagggctg ctatgtgcgg
     9061 caattggccg tttgcttcag ctcaaccagc cggttacgcc aacaaccgtt tgttctacgg
     9121 cggcaagttt ctgccgtggc ctgaccgatc gcgaactgcg taccaataat tcacgactga
     9181 cggcaggtca acggttgtac cagcagcttg gccttaccgg cgcacgcggt gaagccgaag
     9241 cgggttatcc actggtgatc aaccacgcct tgccgcatta cctcactctg ctggatcagg
     9301 ggttagatcc tgaactggca ttgctcgata ccttgctcct actgatggct accaacggcg
     9361 ataccaacgt tgcatcgcgc ggtggcgagg ggggcctgcg ctggctacag cgcgaggcgc
     9421 aaacattatt gcaaaaaggg ggcattcgaa cccccgccga tctcgattat ctccggcagt
     9481 tcgacaggga gtgtatcgaa cgaaatctca gtccaggcgg cagtgccgac ctgctgatcc
     9541 ttacctggtt tttagcacag atttaattat ttaagcactt gataaatttg gaaatattaa
     9601 ttttcggaga acccgtatgt ctttagcaaa agataatata tggaaactat tggccccact
     9661 ggtggtgatg ggtgtcatgt ttcttatccc tgtccccgac ggtatgccgc cgcaggcatg
     9721 gcattacttc gctgtgtttg tggcaatgat tgtcggcatg atcctcgagc caattccggc
     9781 aacagcgatc agttttattg cggttactat ttgcgttatt ggcagtaatt acctgctctt
     9841 tgatgccaaa gaattagctg acccagcgtt taatgcgcaa aaacaggcgc tgaaatgggg
     9901 cctggctggt ttttccagca ccacggtatg gctggtattt ggcgcattta tttttgcatt
     9961 agggtatgaa gtttccgggt taggtcgtcg cattgccctt ttcctggtga aattcatgga
    10021 caaacgcacg ctgacgttgg gttatgcgat tgtcattatc gacattctgc tggcaccgtt
    10081 tacaccgtcc aacaccgcgc gtaccggggg tacggttttt ccggtcatta aaaacctgcc
    10141 gccgttgttt aaatcattcc cgaacgatcc gtccgcgcgt cgtattggcg gctatttgat
    10201 gtggatgatg gtcattagta ccagtctgag ttcatccatg tttgtcaccg gtgcggcacc
    10261 aaacatgctg ggtctggagt tcgtcagcaa aattgccggt atccagatta gctggttgca
    10321 gtggttcctc tgcttcctgc cggttggggt tatcttgctt atcattgcgc cgtggctttc
    10381 ctacgtgctg tacaaaccgg aaatcacaca cagtgaagaa gtggcaacct gggcgggtga
    10441 tgaactaaaa accatgggtg cgctgacacg cagagagtgg acgctgattg gccttgtatt
    10501 gctcagctta ggtttgtggg tatttggcag tgaagtcatt aatgctactg cggttggtct
    10561 gctggcagtt tcgctaatgc tggctctgca cgttgtgccg tggaaagaca ttacccgcta
    10621 taacagcgca tggaacacgc tggtcaacct ggcaactctg gttgtgatgg ctaacggcct
    10681 gactcgttct ggttttattg actggttcgc cggtaccatg agtacgcacc tggaaggatt
    10741 ctcaccaaac gcaacggtga ttgtactggt tctggtgttc tactttgcac actacctgtt
    10801 tgccagcctg tctgcgcaca ccgcaaccat gctgccggtt attctggccg tcggtaaagg
    10861 tattccgggc gtaccaatgg aacaactgtg tatcctgctg gtgctgtcta tcggtatcat
    10921 gggctgtctg acgccgtatg caaccggtcc tggggtgatt atttacggct gtggctatgt
    10981 gaaatcaaaa gattactggc gtcttggcgc aatcttcggg gtgatttaca tctctatgtt
    11041 actgttggtt ggctggccga ttctcgccat gtggaactaa tacgttcgcc aaacgctgaa
    11101 taaatcatca cgcccgccag gtaagcccac ctggcgggct tttttatgat ttaatagata
    11161 gtattcagtg tatgtatgag ttccacaccc attatgaaag cattctgccg taacgccgcg
    11221 ttgctcgcgg tttctctgct tcccttatct tctgccaacg ccgtagcgtt gcaggcaaaa
    11281 cagtatggcg attttgatcg ctatgtcctg gccctctcct ggcaaaccgg attttgccag
    11341 agtcaacacg atcgaaatcg taacgaacga gatgaatgtc gcctgcaaac cgaaacgacc
    11401 aacaaagctg attttctgac cgtacatggt ctgtggccag gattgcctaa atcggttgct
    11461 gcccgtggtg ttgatgaacg ccgctggatg cgcttcggtt gcgctactcg cccaatcccg
    11521 aatctaccag aagcacgcgc cagccgaatg tgttcatcgc cggaaaccgg gttatcactg
    11581 gaaacggccg ctaaactaag tgaagtcatg ccaggagctg gcggacgttc ctgcctggaa
    11641 cgctacgaat atgccaaaca cggtgcctgc tttggttttg atccggacgc atacttcggt
    11701 acgatggtac gcctgaatca agaaattaaa gagagcgaag ccggaaaatt ccttgcggat
    11761 aattacggta aaacagtgag ccgccgtgac tttgacgccg cctttgccaa aagctgggga
    11821 aaagagaacg tgaaagcagt taagctaacg tgccagggta accctgcgta tttgactgaa
    11881 attcagatct cgatcaaagc tgacgccatc aacgctccgc tttctgcaaa ctcattcttg
    11941 ccacaacctc acccaggtaa ctgtggcaaa acctttgtga ttgataaagc gggttattaa
    12001 tcctctgatt tacaaaacac gctaattcgt cttccataac catgccgatt aagaattata
    12061 cataatgcgc tgcattgagg aattccctga atgtgacgca aatcacttca aagagtgagc
    12121 aacagtcagt atcatcatgt gagttaaacc ctcgccgcct gacggtgagg gttttctttt
    12181 gggtatgttt atctgcgaca ctcgcagtac cgacgacatg gagtaaaaat gtccagacca
    12241 actatcatca ttaacgacct ggatgccgaa cgcatcgata ttctgctgga gcaacccgcc
    12301 tatgctggtt tgccaatcgc cgacgcgtta aacgcagagt tggatcgcgc ccaaatgtgt
    12361 tcgccagaag agatgccaca cgacgtggtg acaatgaaca gccgggttaa attccgcaat
    12421 cttagagatg gcgaagtgcg tgtgcgcacg ctggtgtatc cggcaaaaat gaccgatagc
    12481 aatactcagc tttccgttat ggctccggta ggtgccgcac tgctggggct gcgcgttggc
    12541 gattccattc actgggaact tccgggcggc gttgcaaccc accttgaagt gctggaactc
    12601 gaataccagc cagaagctgc tggcgactac ctgctttaat cccatctgca caagcgataa
    12661 ctcgctttgc ccagaggatg cactcgcatc ctctttacgc aaaatcagca atttttcctg
    12721 gttctcactg ttcacttttg ctgcaaaatt attccgtttc gtttgtggga ataaccatgg
    12781 cagaagcatt ttacatcctg ataggatttc tgattatggc ggcgattatc gtcatggctg
    12841 tcctttacct cgaaaaccac tcctgacgat catattgttc cccccggcat cgcattcacc
    12901 agacctgaaa ccgccttctg cgccgcctct gcgctttgtg caccgggtac cagaatcacc
    12961 ttacggcact cctcttcacg tttttcgaaa atctcatatc cccgggcggc ctcttcaaac
    13021 ggcatatagt gggtaacaat ttcttctggt ttcagtaatc ctttctcaat taacggtaat
    13081 aattctccca gccatgcgtg aacgtgggtc tgtcccattt taaacgtcaa ccctttatca
    13141 aaggcgtcgc caaacaggaa accgtgaata aatccagcgt agacgcccgg tacgctaaca
    13201 atgccgccac gcctgaccgc cgcaatacac tggcgcaacg ctttaccgct gctgccctcc
    13261 agtttcaggt tagtcagcac cgtttccgtg gtgctgcctt tcgcttcaaa accgacggcg
    13321 tctattactg catccacgcc ccggtgacct gccgtttgtt caataattga ctgtgccgga
    13381 tcgctatctt catcgaaatt aatcgggatc gcgccgtagc ggtcagcggc gaaacgcaag
    13441 cggtagggat gatgatcaac aacaaaaatc tgttccgcac cgagcaaccg tgcacaggcg
    13501 attgtcaaca atcccacagg accagcacca tagactgcaa cgcttgaacc ttgttggatc
    13561 tgcacatttt ttgctgcctg ccatgccgtt ggcagaatat cagaaaggaa aagggcttta
    13621 tcatctgaaa gcaaaggcgg tactttaaac ggccccacat tccctttagg gacgcggaca
    13681 tattccgcct gcccgctagg aacgccacca tacaggtgac tataaccaaa caatgccgcg
    13741 ggagcaggta tctgtttttt attgagcgca gcgcctttac ccgcattggt attttcgcag
    13801 gcggcatatt gctgtaatcg acagaaaaaa cagtcgccac aagcaatgac gaacggaatt
    13861 accacccggt cgcctttttg caaatttttt acgtccttcc cggtttcaac gacttcccca
    13921 taaattcatg accaaaaata tcgccatgtt taacctgggg tattttgcct cgataaagat
    13981 ggaggtcaga gccacagatc gccgttaccg taatacgcag aataatatca tctgcctgtt
    14041 caatgcccgg atcgggaaca ttttctacct gaacgtgatg tgggccgtga taagtcaatg
    14101 ctttcatgca acctccagag acagttcaaa aaagagtcag tctttaaggg tagctatcac
    14161 agcgggaaaa cgggcggtac cgcaggtttt gggagaattc ctggtgttca gtgagaatat
    14221 attaatcaca gaaaccgttc gtaaggaaaa agaaccagac aaatatcagg gataaatatt
    14281 ataatcatgg cgaatgattt gttcatgatt aacagggaga aagggttatg tataaggcaa
    14341 tcattatgcc agttgatgta tttgaaatgg aattgagcga caaagctgtt cgccacgctg
    14401 aattcctcgc ccaggatgac ggagttattc atctacttca cgtactaccc gggtcagcca
    14461 gcctgagcct gcaccgtttt gccgctgatg tgcgtcgttt tgaagagcat ctgcaacatg
    14521 aagcagaaga acgtctgcaa acgatggtca gccacttcac catcgatcct tcccgcatta
    14581 aacaacatgt ccgttttggt agcgtgcggg atgaagtcaa tgagttggca gaagaactgg
    14641 gggctgatgt tgtagttatc ggttctcgca acccatcgat ttcgacccat ctgttaggtt
    14701 ctaacgcctc gagcgtgatc cgccacgcca atctgcctgt actggtcgtt cgctaacaga
    14761 taaaagtatt tatgccgccc tgcctggaac atcccggcag ggctttttaa tgccataaaa
    14821 aagccgccag gtttgacccc ggcggctaag caattgcagg tgaatcttac ttcttcttat
    14881 gcagttttgg tgcgaatcag gtagtcaaaa gcactcagag aggctttggc accttcgcca
    14941 gtggcgatga tgatctgctt gtacggaacc gtcgtacagt cacccgctgc gaacacgcct
    15001 ttgacgttgg tttcacattt cgcatcaatg ataatctcgc ccatgcggtt acgttcgact
    15061 gcgccttcca gccagttggt gttcgggagc agaccaatct ggacaaaaat accggccagt
    15121 tcgatgttgt gaatattgcc gctgacacga tctcgatatt ccagaccaac gactttgctg
    15181 ccgtcgcctt tcacttccgt ggtttgcgca ttcagaataa tgtcgacgtt tttcaggctg
    15241 cgcagtttgt cctgcagaac ctggtcggct ttcatttctg gcgcaaattc cagcagcgtt
    15301 acgtgctcaa cgatacccgc caggtcgatt gccgcttcca cgccggagtt accgccgccg
    15361 ataaccgcta cgcgtttgcc tttaaacagc gggccgtcgc agtgcgggca gtaggtcacg
    15421 cctttagtgc gatactgatc ttcgcccgga acgttcatgt tgcgccattt tgcacccgtc
    15481 gccacgataa tgctgcgtgc tttcagtacc gcgccagaag ctgtttcaat ctggtgtaga
    15541 ccaccttcaa ccgctgccgg gatcagtttg ctggcgctct ggctgtcgat cacatcaacg
    15601 tcgtattcat caacgtgaac tttcagtgcg cctgccaact tctgcccttc agtcttcggt
    15661 acagaaatgt agttttcgat atcaacggta tcgaggatct gaccaccaaa acgttcgccc
    15721 atcagaccgg tacggatgcc tttacgtgcg gagtaaattg ctgccgctgc acccgccggg
    15781 ccggaaccaa cgattaatac gtcataagca tcacgcttgt tcagctcttc tgccgcacgt
    15841 ttttccgcgc cagtatcaat tttggcaacg atttcagtca acgtcatgcg gccctgacca
    15901 aactctttcc cgtttacgaa cactgccgga acgcccatca cgttgcgatc ggtgatttcg
    15961 ttctggaagg tgccgccgtc aattgcagtg tgcttgatgc gcgggttcaa tacgctcatc
    16021 aggttcagcg cctgcaccac gtccgggcag ttatggcaag agagcgagta ataggtttcg
    16081 aattcaaaat caccgtcaat atggcgaatc tgctccagca gagactgcgc ttctttcgac
    16141 ggatgaccac cggtccacag caacgccagt accagcgagg tgaactcgtg gcccagcggg
    16201 gagcctgcaa aacgtggtcc ctggttggaa cctgggttgg tgatcaggaa agacggctta
    16261 cgcactggca agctgttatc ttctttaaag gtgactttgt ctgacagttc tgcgatttca
    16321 gccaacagtt ccttgatttc tgccgattta gcgctgtcat ccagcgtggc aattaactca
    16381 acaggcttgg tcaatttctc aaggtaagcc ttgagttgag ttttcatatt tgtgtcgagc
    16441 atgtttatct cctgggcttt aaacatcatc atgcaagcgg cctctgcggc tacctggatg
    16501 cagcttgcat catggtgcgg ggaagaaaat cgggtgccgc agcacccgaa gaattaggtg
    16561 aatttccgaa gtatttcgcg tcagagcaag gcgactagcc tgtgaatccc cgggagctta
    16621 cacaagtaag tgaccggggt gagcgggcga cgccaacgcc gctatggcgt gaaagacgaa
    16681 ggaaatttag attttaccaa ccaggtccag agacggagcc agagttgctt caccttcttt
    16741 ccatttagcc gggcaaactt cacctgggtg agaagctacg tactgagctg ctttgatttt
    16801 acgcagcaga tcagacgcgt cacggccaat gccttcagcg gtaacttcga ttgcctggat
    16861 gataccctgc gggtcaacaa cgaaggtcgc acggtcagcc agaccttcat cttcacgcat
    16921 gttgtcgaag ttacgggtca gggcgccagt cgggtcgccg atcatcgcat atttgatttt
    16981 agcgatggtt tcagagctgc tgtgccatgc tttgtgggtg aagtgagtat cggtagatac
    17041 tgcgtatacg tctacgccca gtttctgcag ttcttcgtag tggtcagcaa cgtcacccag
    17101 ttcggtcggg catacgaaag taaagtcagc cgggtagaag aagaagacgc tccagcggcc
    17161 ttcggtatct ttttcggtga tttcgatgaa ttcgccgttt ttgaatgcct ggtttttaaa
    17221 aggtttaatt ttggtgttaa tcaaggacat ctatacttcc tccgtgtttt cgatgagatg
    17281 taaggtaacc aatttttgcc gattcggcta atgcgtttcc attatcaaat cgataagttt
    17341 taccttacaa ccttcgtaag acaactttgt gaactttgca ccgccagcgg cgataaaaag
    17401 taaaatggcc ccctttccag gtgaccgtgt aatctgaact accctttggt atcttcagcg
    17461 cctgcctgca ggctgaggtg ttgcccttac aaatgcaaca acgacatgga ttacaacacc
    17521 ctcataaaca aagggcaatc acctgatcta agctcttacc tatgacagtg ataggttatg
    17581 ccttttactc gacttttgca ctgactgaaa agaacaaatt aatgttaaaa aagatacttt
    17641 tactggctct gcttcctgca atcgccttcg cagaggaact tccttcccca gtaaaagcga
    17701 ttgaaaaaca tggcattaca atcatcaaaa cgttcgatgc ccccggagga atgaaaggtt
    17761 atctcggaaa gtatcaggat atgggcgtca ccatctacct gactcccgat ggtaagcacg
    17821 ctatctctgg ttacatgtac aacgagaaag gtgaaaacct gagtaacaca cttatcgaaa
    17881 aagaaattta cgcaccagcc ggacgcgaaa tgtggcaacg gatggaacaa tcccactggc
    17941 tcctcgacgg taaaaaagat gcgccggtca ttgtctacgt cttcgccgat ccgttctgcc
    18001 catattgtaa acagttctgg cagcaggcgc gcccgtgggt agattctggc aaagtgcaat
    18061 taagaacatt gttggttggg gttatcaagc cagaaagccc ggcgacagca gcggcaattc
    18121 ttgcctccaa agatcccgca aaaacctggc aacaatatga agcctctggt tgcaagctta
    18181 agctaaacgt gcctgcaaac gtaagtacag agcaaatgaa agtgttaagt gacaatgaga
    18241 aactgatgga cgatctgggg gcaaatgtca cgccggctat ctattacatg agtaaggaaa
    18301 atacgctgca acaggccgtg gggttgcccg atcagaaaac gcttaatatc attatgggaa
    18361 ataaataaaa ggtaattcat ggatggttga actatatccc tgcttttcaa ccatcctaaa
    18421 aaagcatgaa ttgctcgctc taccctaagt ggctgtgcta ttttagaaaa acttaatcct
    18481 gagttaagca aaaaaacata atcaataaaa atacatacgc aaaaaacatt gattaagtga
    18541 atataccatg gaagaaaaat ataaaccgga gtagtgtatg gccaatctct acgacttgaa
    18601 aaagttcgac ttaaatcttc ttgtcatatt tgagtgtatt taccagcatt taagcattag
    18661 caaagctgct gaatcactat atatcacccc atcagccgtg agccagtcgt tacaacgctt
    18721 acgtgcgcaa tttaatgacc ctttattcat tcgctcagga aaaggtatcg cgccgacgac
    18781 aacagggttg aatctgcatc accatcttga gaaaaacctc aaaggccttg aacaaacaat
    18841 taatatagtc aataagtccg aactaaagaa aaactttatt atctacggcc ctcagctcat
    18901 ttcttgctct aataatagta tgctgatccg ctgcctacgg caagatgcct ctgtcgagat
    18961 tgagtgccat gatatcctca tgtcagcaga gaatgctgaa gaactgttgg ttcaccgcaa
    19021 agccgatctg gtgatcaccc aaatgccagt gatcagccgt tccgtcattt gcatgccatt
    19081 gcatacaatt cgcaatactc taatttgtag taataagcat ccgcgaatta ctgacaatag
    19141 cacttatgaa caaattatgg cagaagagtt cacccaatta atatccaaat cagcaggagt
    19201 tgatgatatt caaatggaaa tagacgagaa gtttatgaat cgcaaaattt catttcgtgg
    19261 ttcctctttg ctgacaataa ttaatagcat tgcagttaca gatttactgg gaatagtccc
    19321 ctatgaactt tataactacc accgtgactt tcttaattta aaagagataa aactagaaca
    19381 tccgttacct tcaataaagc tttacatttc ctataataaa tcttctttaa ataatttggt
    19441 gttctcccga tttattgacc gtctaaatga gagtttttga tatataacta cagacagcta
    19501 ctataacttt catctattta ttcacagctc gaatgaaaaa taaaacactc tgctttacag
    19561 tgtttacttt ataattaaca gtcttatctc actgttcacc ctgtaacgtt cagagagcgt
    19621 cttcagatgt ctatttataa aattcctctt ccactcaata ttctggaagc tgcacgagag
    19681 cgtattacct agacactcaa caccctaccc cgcgtttgtg tctcattttc tggcggcaaa
    19741 gactctggtt taatgctgca tctgacagcc gaacttgccc gacaaatggg caaaaaaatc
    19801 tgcgttttgt ttatcgactg ggaggc
//
LOCUS       DAJVQH010000072        19664 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJVQH010000072 DAJVQH010000000
VERSION     DAJVQH010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.072"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            4..270
                     /locus_tag="OQM87_002557"
     CDS             4..270
                     /locus_tag="OQM87_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1508647.1"
                     /translation="MNNPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVV
                     TLIDDNIREEILRPEWIAGETGMSVRSLYRMFADIRFGSRAIYS"
     gene            245..436
                     /locus_tag="OQM87_002558"
     CDS             245..436
                     /locus_tag="OQM87_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1508648.1"
                     /translation="MVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFS
                     TVFKQRFGMTPGEYRRKFR"
     gene            complement(608..937)
                     /locus_tag="OQM87_002559"
     CDS             complement(608..937)
                     /locus_tag="OQM87_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCS1508649.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(942..1127)
                     /locus_tag="OQM87_002560"
     CDS             complement(942..1127)
                     /locus_tag="OQM87_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCS1508650.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1124..3763)
                     /locus_tag="OQM87_002561"
     CDS             complement(1124..3763)
                     /locus_tag="OQM87_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCS1508651.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3971..4960
                     /gene="ldhA"
                     /locus_tag="OQM87_002562"
     CDS             3971..4960
                     /gene="ldhA"
                     /locus_tag="OQM87_002562"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1508652.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5071..5418
                     /gene="hslJ"
                     /locus_tag="OQM87_002563"
     CDS             5071..5418
                     /gene="hslJ"
                     /locus_tag="OQM87_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCS1508653.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEISFGEKMMISGSMCNR
                     FSGEGKLSNGELTAKGLAMTRMMCANPQLNELDNTISEMLKEGAQVDLTANQLTLATA
                     KQTLTYKLADLMN"
     gene            complement(5415..5681)
                     /locus_tag="OQM87_002564"
     CDS             complement(5415..5681)
                     /locus_tag="OQM87_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCS1508654.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5955..9479
                     /gene="nifJ"
                     /locus_tag="OQM87_002565"
     CDS             5955..9479
                     /gene="nifJ"
                     /locus_tag="OQM87_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCS1508655.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9846..10975
                     /gene="ompN"
                     /locus_tag="OQM87_002566"
                     /pseudo
     CDS             9846..10975
                     /gene="ompN"
                     /locus_tag="OQM87_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            11116..11550
                     /gene="uspF"
                     /locus_tag="OQM87_002567"
     CDS             11116..11550
                     /gene="uspF"
                     /locus_tag="OQM87_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCS1508656.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11726..12661
                     /gene="ttcA"
                     /locus_tag="OQM87_002568"
     CDS             11726..12661
                     /gene="ttcA"
                     /locus_tag="OQM87_002568"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCS1508657.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12790..14157)
                     /gene="dbpA"
                     /locus_tag="OQM87_002569"
     CDS             complement(12790..14157)
                     /gene="dbpA"
                     /locus_tag="OQM87_002569"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCS1508658.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTLALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14635..15618)
                     /gene="zntB"
                     /locus_tag="OQM87_002570"
     CDS             complement(14635..15618)
                     /gene="zntB"
                     /locus_tag="OQM87_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCS1508659.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            15873..17105
                     /gene="dgcM"
                     /locus_tag="OQM87_002571"
                     /pseudo
     CDS             15873..17105
                     /gene="dgcM"
                     /locus_tag="OQM87_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(17126..17689)
                     /gene="smrA"
                     /locus_tag="OQM87_002572"
     CDS             complement(17126..17689)
                     /gene="smrA"
                     /locus_tag="OQM87_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCS1508660.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17943..19397)
                     /locus_tag="OQM87_002573"
     CDS             complement(17943..19397)
                     /locus_tag="OQM87_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCS1508661.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGILQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(19394..>19664)
                     /locus_tag="OQM87_002574"
     CDS             complement(19394..>19664)
                     /locus_tag="OQM87_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1508662.1"
                     /translation="KENQIAGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLI
                     PIENATGNFTKIVQRVPVRIAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         5172 a         4974 c         4637 g         4881 t
ORIGIN      
        1 agcatgaata atccggcact ttctgaaaca gaaagtgaag ctgcgctaca ggcgatggtg
       61 tgtctgcttc gcccagtact tcatcagcgg gaatctgtcc aacctcgccg tgaacgtcag
      121 tttcaaaaag tggttacgtt gatagacgat aatattcgcg aagagatatt acgcccggag
      181 tggatagccg gagagacagg tatgtcagta cgtagtttgt accgaatgtt tgccgatata
      241 aggtttggta gtcgcgcaat atattcgtaa ccgtcgtctc gatttttgtg cagatgcgat
      301 tcgccatgcc gcagatgatg aaaaactggc aggcatcggc tttcattggg gattttctga
      361 ccagagtcat ttctcaacgg tatttaagca acgctttggg atgacgccag gcgagtatcg
      421 ccgtaaattc cgctaactaa ttggcgttgc atgtactgca acgccaatta gttaaaacag
      481 atatttgact atgcattttt cagggcgaaa ggcaatttga caggagtttt tgaatattca
      541 ccttgtttca ggaaaataga tcaatcgctt tctcctgtag cgttatcccg atattgcggt
      601 tttgctatta aaactttcgc acccatttac cgtttatccc ttgtacatat tcccccggtt
      661 tggcccgagc tacgagcttt tgcccggcga gtttcgcgat atcatctaca gacacattat
      721 tttgctttgc ccgttgttgg taactcgctt tacgttcggc attaatttcg gttactaatt
      781 tctctgtttc agcatccgtt ttcagcgcaa ccagataacc ataaaatgtt tcacctaccc
      841 gcccctgagt tctggcttca tccagagtca atgccattac attgctgctt accagcccaa
      901 caagaaacgc acaaagaagt aatgttttct tcatcatcgc ctcagaaaag atcgctacga
      961 gtttcaagca gctcttcgac atctttgtct gccttgatga tgatctcatg ctcaatttta
     1021 acgttcatat tgatagtgat cggttcctta ggtgcagcga cttcaatgcg aggcgtacag
     1081 ccaaccagca taaaagatga cgtcaacgca gccagtaaaa ttttcattgt ttttcctcac
     1141 actcttttcc ttgcggacag tcatttcccg gcagacgtgc gttctgctcc aaccatgcct
     1201 ggagattatc gccaaagcgt aaactgcgcc acagcgtaaa aatattctct tcatgatggt
     1261 aattaaggtt taccgtaccg cttttaccat caacgcgact ggtacctgta atgttggcct
     1321 gcatggttaa taaaccgaga ttatctaagt taatttttgt cgatgaccgg ctaatttcca
     1381 tatagcgcaa ccagttaatt gctgaacccg cagtcatatt gtctttcacc accgcatctg
     1441 cggtatcttt atccagccgc aaggtcattg gcccgctatt tgccagccaa ccatctttca
     1501 ctatccattt ttcgttgttt agccataacg gcaatgcacc actaaatgcc ccggacatgg
     1561 cgaattgttt cggattgacg gcgctaacca gttcgctgga tgagagatta ttcagccgca
     1621 acagcgccgg gtcatgttgc ggcatacgta attgtttcat cagcacgtta ccgcctaaca
     1681 catcgacgct aacatcagtc aacagcaagg gttcttcttc agtccacgga taacgccctt
     1741 gcaaatcagc cgtaatattt ttcgctgtca ccagattaat cacttcggca attcgcaacg
     1801 taacggggcc gcgagtcccc agatgccagg ctccatcggc aaaacggaaa ggcaggacaa
     1861 aatcgacacc gttaacctga ttatctggca tccaggcact accgcctttc aacacgccgt
     1921 gccctcccgc gcggaatcct tgttcaggcg cagcagaaaa tgcaacctga gcatacagtt
     1981 caccatcgcg taagttcatc ttccagtcgg gtggcaccag cggctggaat acagtcagtg
     2041 attgttttgg ccaccaggcg ttaccgcgca gacgaatacc gtcccagcga ccatttaccc
     2101 gaaccgggcc aatctcacca acatgtaaat cgcctttaaa gaggaaatag gtcggatcgc
     2161 gcccatcgac gctaaatttt aaggttgatg gcggtaacac actgccgcca gtgaacagcg
     2221 tttgcccggc gtccagtgac agcgcgccgc tgaaggaggg atgctgtgcg tcacgtaccc
     2281 agacgacggg cttgtcgaga attaatcgcg gcttttctac cgtcatcgta ccgtattgta
     2341 actggtcgaa accggtggag agatcggtca gcgtaatcgt gctgtcatgc cactcacctt
     2401 ttcctgcgac atcccaggtg gcattcatcg gtgtaaaact cccttttccc cagtagcgcc
     2461 actgccagcg gccagcgtca gggagaaaat cattcgccag cccatccata tgcagcacga
     2521 aatcgcccag ttcattttca tgcgcctgca aaatggcctg caaacgtccg tcaacaccac
     2581 gttgggtgac ttttacaccc gctaaaggcc agcggatttc atcgatgtcc agcgaatcga
     2641 tgactcttcc ctttgaacga agtaacgcgc ctggctcaaa ggtcagcgtt gggtcagaca
     2701 gacttccact tagctgcgca ggtaaacggg catataaaat gaggtccgcc tgtttcgctt
     2761 caccggtcag ctgcagaggc agttgactgt tatccatgct taattttcct gggccaaaat
     2821 taagcaccgc gttgccctta cccgcttgcc cctgggtcag cacactcagt cgtccgctga
     2881 ccagagcgtt ctcaagccct gcctgccagt tgtcaacttt gacacccagt cggccactca
     2941 aaggaaaacc tgcatacagc cagctccagc gaccatcgct tacggtcaat tgttgacgag
     3001 taatttgcca cggcaaatcg agcaacggat cgccgttatc ccgtgccagc acgatcaatt
     3061 gcccgctatt ttcctgccag tccagctcgg catccaccag tgacggttcc tgcggcaagt
     3121 ttaacgtcgc agtagcatgc ccacttacag gaagtccatc cggcacgagc ggcatagtaa
     3181 attcccccac cagtttaacc ggcggctgat tttcaaacgc gacgacatcc agttcgctga
     3241 ctgtaagttg ttgccctttc agctggcctt gaaatttaac tttttcgccc tgataacgca
     3301 gttgctggat atcagaggtt aatgcgagag agagttttcc ctgccattcc tgccagggag
     3361 aaaaaatcag tttatcgata ttgatccagg tgttaggcag catggactgc cactgcgcga
     3421 gggtttttgg agcagctggc gattgctccg tctgcggcaa tttcgccaga caagcagaat
     3481 caagttctac cgtgccgacg ttcaataacc agcggctggg atgtgaaagg ctggcgttgg
     3541 tgatatgcgc aagctgacaa tctcccacca gataacggag atcgggaatg attaaacctt
     3601 tacgcgtaat gcgtgggctt tcatctaatg caatacgtgt cccgagcggt agccagatgt
     3661 ccgccagcgt gggaacccac agcccgagcg tcatcagcag cgtcaacggc acaagaataa
     3721 tcagtaataa cagcgcgaga acggctttat atttacccag catgggtagt taatatcctg
     3781 atttcgcgaa aaattaagca ttcaatacgg gtattgtggc atgtttaacc gttcagttga
     3841 aggttgcgcc tacactaagc atagttgttg atgaattttt caatatcgcc atggctttca
     3901 attatatttg aaattttgta aaatattttt agcagcttaa atgtgattca acatcactgg
     3961 agaaagtctt atgaaactcg ccgtttatag cacaaaacag tacgacaaga agtacctgca
     4021 acaggtgaac gagtcctttg gctttgagct ggaatttttt gactttctgc tgacggaaaa
     4081 aaccgctaaa actgccaatg gctgcgaagc ggtatgtatt ttcgtaaacg atgacggcag
     4141 ccgcccggtg ctggaagagc tgaaaaagca cggcgttaaa tatatcgccc tgcgctgtgc
     4201 cggtttcaat aacgtcgacc ttgatgcggc aaaagaactg gggctgaaag tagtccgtgt
     4261 tccagcctat gatccagagg ccgttgctga acacgccatc ggtatgatga tgacgctgaa
     4321 ccgccgtatt caccgcgcgt atcagcgtac ccgtgacgct aacttctctc tggaaggtct
     4381 gaccggcttt actatgtatg gcaaaacggc aggcgttatc ggtaccggta aaatcggtgt
     4441 ggcgatgctg cgcattctga aaggttttgg tatgcgtctg ctggcgttcg atccgtatcc
     4501 aagtgcagcg gcgctggaac tcggtgtgga gtatgtcgat ctgccaaccc tgttctctga
     4561 atcagacgtt atctctctgc actgcccgct gacaccggaa aactaccatc tgttgaacga
     4621 agccgccttc gatcagatga aaaatggcgt gatgatcgtc aataccagtc gcggtgcatt
     4681 gattgattct caggcggcaa ttgaagcgct gaaaaatcag aaaattggtt cgttgggtat
     4741 ggacgtctat gagaacgaac gcgatctgtt ctttgaagat aaatccaacg acgtgatcca
     4801 ggatgacgta ttccgtcgcc tgtctgcctg ccacaacgtg ctgtttaccg ggcaccaggc
     4861 attcctgaca gcagaagctc tgaccagtat ttctcagact acgctgcaaa acttaagcaa
     4921 tctggaaaaa ggtgaaacct gcccgaacga actggtttaa tcttgccgct cccctgcaat
     4981 ccaggggagc tgattcagat aatccccaat gacctttcat tctctattct taaaatagtc
     5041 ctgagtcaga aactgtaatt gagaaccaca atgaagaaag tagccgcgtt tgttgcgcta
     5101 agcctgctga tggcgggatg tgtaagtaat gacaaaattg ctgttacgcc agaaatcagc
     5161 tttggtgaaa aaatgatgat ttccggcagc atgtgtaacc gctttagcgg tgaaggcaaa
     5221 ctgtctaatg gtgaactgac agccaaaggg ctggcaatga cccgtatgat gtgcgctaac
     5281 ccgcagctta atgaactcga taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg
     5341 gatctgaccg cgaaccagtt aacgctggcg accgcgaaac agacattaac ttataagctg
     5401 gcggatttaa tgaattaata gctgccacag ctcccggcgg caagtgactg ttcgctacag
     5461 cgtttgccgt tgggtaatgc acacatccca atcgccgtac catccagttg acgggcaaca
     5521 gaaagcgaac cgccgatcat tgcacaattt gcttctccac tactggacat cgacgctttt
     5581 aaacttggcg ctacgtgcgc cgcagtcgcc tgctgaacag gctcactact acacgccgac
     5641 aacaataaag cggcacaccc tacccaaaac gctgctcgca tctcttcttc ctctgatctt
     5701 caagccaaac gacaccgcca taaataatag gcagcacaga gggcggcgtc gagagctgtc
     5761 ctgcgcgttg ccccgcattt ttactttttt atggctattt ttttgccctc tgtttgatca
     5821 aaacattcat tactctgatg tgggggacac aaaagcgaaa atgcagaaga aagccatttg
     5881 ctaaaattga aggattacta ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa
     5941 tgtaagggtg tcatatgatt actattgacg gtaatggcgc ggttgcttcg gtggcgtttc
     6001 gcaccagtga agttatcgcc atctacccta ttacccccag ttccacgatg gcagaacagg
     6061 ctgatgcctg ggccggaaac ggcttgaaga acgtttgggg agacacacca cgcgtggttg
     6121 aaatgcagtc ggaagcgggt gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc
     6181 tttcaacatc gtttacgtca tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac
     6241 tggcaggcga actgacgcca tttgtcctgc atgtagcggc acgtaccgtt gccacacatg
     6301 cactttctat ttttggcgat cattccgacg ttatggcggt gcgccagacg ggttgcgcga
     6361 tgttgtgtgc ggctaacgtc caggaagcgc aagactttgc tctcatttca cacatcgcaa
     6421 cgctgaagag ccgcgtgcca tttattcatt tctttgatgg ttttcgcacg tcccacgaaa
     6481 tcaataaaat tgtcccgctg gccgatgaca ctattcttga tctcatgccg caggccgaaa
     6541 ttgatgctca tcgcgcccgg gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg
     6601 ccaatcctga cacttatttc cagtctcgcg aagccaccaa cccctggtac aacgcggtct
     6661 atgaccatgt tgaacaggcg atgaatgatt tcgctgccgc gacaggtcgt cagtatcagc
     6721 cgtttgagta ttacgggcat ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca
     6781 ttggcacctg tgaagaagtg gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc
     6841 tgaaagttcg tctgtaccgt cccttctccg ccaaacactt gctgcaagct ctgccgggat
     6901 ccgtacgcag cgtggcggta ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc
     6961 tctatctgga tgtgatgacc gcactggcag gagcctttaa taatggtgag cgcgaaaccc
     7021 tgccccgtgt cattggtggg cgctatggtc tttcatccaa agaatttggc ccggactgtg
     7081 tactggcggt atttgccgag ctcaacgcgg ctaaacctaa agcacgcttt acggttggta
     7141 tttacgatga tgtgaccaat ctgtcactgc cgttgccgga aaacactctg ccaaactcgg
     7201 cgaaacttga agccttgttt tatggccttg gtagtgatgg cagcgtttcc gcaaccaaaa
     7261 acaatatcaa gattatcggt aattccacgc cgtggtacgc acagggctat tttgtttacg
     7321 actccaaaaa agcgggcggc ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc
     7381 gttccgctta tctcatttcc caggctgatt ttgttggctg ccaccagttg cagtttatcg
     7441 ataaatatca gatggctgag cgtttaaaac ctagcggcat tttcctgctt aatacgccgt
     7501 acagcgcaga tgaagtgtgg tcgcgcttgc cgcaggaagt tcaggccgtg ttaaaccaga
     7561 aaaaagcgcg cttctatgtg attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg
     7621 cccgtattaa taccgtcatg cagatggctt ttttccatct gacgcaaatt ctgcctggcg
     7681 atagcgccct cgcagaattg cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg
     7741 atctggtgga gcgcaactgg caggctctgg cgctggcgcg tgaatccgta gaagaagttc
     7801 cgttgcaacc ggtaaatccg cacagcgcca atcgaccgcc agtggtttcc gatgccgccc
     7861 ctgatttcgt gaaaaccgta accgcggcga tgctcgccgg gcttggtgat gccctccccg
     7921 tttcggcgct gccgccagac ggcacctggc cgatgggcac cactcgctgg gaaaaacgca
     7981 atatcgccga agagatcccc atctggaaag aggaactctg tacccaatgt aaccactgcg
     8041 tcgccgcttg cccacactca gctattcgcg caaaagtggt accgcctgaa gcgatggaaa
     8101 acgcccctgc cagcctgcat tcgctagatg tgaaatcgcg tgatatgcgc gggcagaaat
     8161 atgtcttgca ggtggcaccg gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc
     8221 cggcgaaaga ccgtcagaat ccagagatta aagccatcaa tatgatgtct cgccaggaac
     8281 atgtcgaaga agagaaaatc aattacgatt tcttcctcaa cctgccagaa atcgaccgta
     8341 gcaaactgga acgtattgat attcgtacat cgcagctgat tacaccgctg tttgaatatt
     8401 caggtgcttg ccccggttgt ggcgagacgc cgtatattaa attgctgact cagctctatg
     8461 gtgaccggat gttgatcgct aacgccaccg gctgttcttc aatttatggc ggtaacctgc
     8521 cctctacacc gtataccacc gatgccaacg gtcgtgggcc ggcatgggcg aactctctat
     8581 ttgaagataa tgccgaattt ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc
     8641 gcgtgctgcg tctgctggat caatttgccg ataaaatccc ggcggaatta ctgacggcgt
     8701 tgaaatcaga cgctacgcca gaggttcgtc gagaacaggt cgcagcttta cgccagcaac
     8761 tcaacgatgt tgccgaagca catgaactgc tgcgtgacgc agatgcactg gtggaaaaat
     8821 caatctggct gattggtggc gacggctggg cttacgatat cggctttggc ggtctggatc
     8881 atgtattgag tttgacggaa aacgtcaaca ttctggtgct ggatacgcaa tgctattcca
     8941 acaccggtgg tcaggcgtcg aaagcgacac cgctgggtgc agtaactaag ttcggcgagc
     9001 acggcaaacg taaagcgcgt aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt
     9061 atgtggcgca gatttctctc ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag
     9121 cggaagcgta tccgggacca tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt
     9181 acgatctggc tctcagccac gaccagatgc gccaactcac tgctaccggc ttctggccgc
     9241 tatatcgctt tgatccgcgt cgtgccgatg aaggcaaact gccgctggcg ctggattcac
     9301 gcccgccgtc agaagcactg gaagaaacgt tacttcacga gcagcgtttc cgtcgtctga
     9361 attcgcagca gccagaagtg gcagaacagt tatggaaaga tgctgcagct gatttgcaaa
     9421 aacgctatga cttcctagca caaatggccg gaaaagcgga aaaaagcaac accgattaaa
     9481 tgctctggat aaggattatc caattctaaa aaaaaagccc ggacgactgt tcgggcttgt
     9541 ctttttatat gttggaaaat cagtggcaat gcaatggccc aacagaaaat aaaatctgtg
     9601 acaataaagg catataaccc gcgcagaata acgtatagaa aattaaaagt ataattttta
     9661 ttttgtatgt attatcttcg atacattcac cagacttatt atcatttcag aggaattatt
     9721 tgattaaggt tttacttaag gcgtaacaaa tgatatttat cggcaaactg aacttctcct
     9781 ttaggatgtt ttcactcccc gaaatgggga taaaaggcaa ataaaataac taaaggattt
     9841 attcaatgaa aagaaaagta ctggcacttt taatccctgc cctgctcgcc gcaggtgctg
     9901 cacatgcagc cgaagtttat aataaagacg gcaacaaatt agatctgtat ggcaaagttg
     9961 atggcctgca ttatttttct gataattcag cgaaagatgg cgaccagagc tatgcgcgtc
    10021 tgggttttaa aggcgaaacc caaattaacg atcaacttac tggctacggt caatgggaat
    10081 acaatattca ggcaaacaac actgaatctt caaaaaacca gtcatggacc cgtctggcat
    10141 ttgccggact gaaatttgca gattacggtt ctttcgatta cggacgtaat tatggcgtaa
    10201 tgtacgacat cgaaggctgg accgatatgc tgcctaaatt tggcggtgac tcttatacca
    10261 atgcagacaa ctttatgact ggtcgagcca atggcgtcgc gacttatcgt aatactgatt
    10321 tcttcggtct ggtaaatggt ctgaacttcg cggtgcagta tcaaggtaac aacgaaggag
    10381 ccagtaatgg tcaggaaggc accaataacg aacgcgatgt tcgtcatgaa aacggtgacg
    10441 gctggggtct ttccacaaca tatgatttag gcatgggctt tagcgctggt gcggcataca
    10501 cctcttctga ccgcaccaat gaccaggtta accatactgc ggcgggtggt gataaagcag
    10561 acgcgtggac tgctgggcta aaatacgatg ctaacaatat ttacctagca accatgtatt
    10621 cagaaacgcg taatatgacc ccgtttggcg acagcgatta tgcagttgcg aacaaaaccc
    10681 agaactttga agttactagt accagtttga ttttggtctg cgtccggcag tctctttcct
    10741 gatgtctaaa ggccgtgacc ttcacgctgc tggtggtgca gacaacccgg caggtgttga
    10801 tgataaagat ctggttaaat acgccgatgt tggcgcgact tactatttca ataaaaacat
    10861 gtccacctat gttgactata aaatcaacct gttggatgaa gatgacagtt tctacgctgc
    10921 caacggcatc tctaccgatg atattgtcgc tttaggtctg gtttatcagt tctaaatcct
    10981 cctgcccgct gttatggcgg gctttttctg cttattcttc ctcctttgat ctaaattaaa
    11041 aatgtgaact ccgtcattac acaaaaagtg tcatctggcg ttacacttta tgcggatact
    11101 aaaacaggag gttttatgaa cagaacgatt cttgtcccta tcgatatttc agattcagaa
    11161 ttaactcaac gcgtgattag ccacgttgag gcagaggcaa agattgatga tgcagaggtt
    11221 catttcctga cggtaatacc ttcactgccc tactatgcct ctctgggttt agcgtattcc
    11281 gcagaattac cggcaatgga tgacctgaaa gcggaagcca aatcgcaact ggaagagatc
    11341 attaaaaaat ttaaactgcc aaccgacaga gtgcatgtcc atgttgagga aggctcgccc
    11401 aaagaccgca ttctggaatt ggcgaagaag atccccgctc atatgatcat cattgcttcc
    11461 catcgaccgg atatcaccac ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac
    11521 gcagagtgct ccgtgctggt tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt
    11581 ttgattcatt tcgcaaatgt gctgacattt tcccctctaa tccgtaccat acacgccaca
    11641 gtttttatat cagatttctt atgctgagcg ttccggcatg acgcatactc ttctgatgcc
    11701 atataacgaa ttgagtcgct tttaaatgtc gcaaaatcaa gaaattagta agaaagaaca
    11761 atacaacctg aacaaattac aaaaacgtct gcgtcgtaac gtgggcgaag ccattgctga
    11821 cttcaatatg attgaagaag gcgatcgcat catggtttgc ctctccgggg gtaaagacag
    11881 ctataccatg ctggagattc tgcgcaattt gcagcaaagc gcgccaatca atttttcgct
    11941 ggtggctgtt aacctcgatc aaaagcaacc gggcttcccg gaacacgttc tgcccgagta
    12001 tcttgaaaag ctgggcgttg agtacaagat tgttgaagag aacacttacg gtatcgtgaa
    12061 agagaagatt ccagagggca aaaccacttg ctcactgtgt tctcggctac gtcgcggtat
    12121 cctttatcgc accgcaacgg aactggggac gacgaagatc gcgttgggtc accatcgtga
    12181 cgatatcctg caaacgttgt tcttaaatat gttctacggc ggcaagatga aaggtatgcc
    12241 tccgaaactg atgagcgatg atggcaaaca tatcgttatt cgtccactgg cctactgccg
    12301 cgagaaagat attcagcgat ttgccgatgc aaaagcgttc ccgattattc cgtgcaacct
    12361 gtgcggttca cagcctaacc tgcaacgtca ggtgattgct gacatgttgc gtgactggga
    12421 taaacgttat ccagggcgta tcgagacgat gttcagcgcg atgcagaatg tggtgccgtc
    12481 gcatctgtgc gataccaacc tgttcgattt caaaggcatt acccacggtt ctgaagtggt
    12541 taacgggggt gatctggcgt ttgatcgcga agagatccca ctacaaccgg cgggctggca
    12601 gccagaagaa gatgaaaatc agttggatga gttacggctg aatgtggttg aagtgaaata
    12661 accaggatag cgcccgatgc gcaagcgtat cgggctactc ttatggaggc cggataagac
    12721 gcggccagcg tcgcatccgg caatcccgaa taagatgttt actcttgcac ccggcaattc
    12781 aacatttcat tattttaata accgcacccg gcacgttttt cctttaatct tcccgccctg
    12841 taactgtttc catgctttat gagcaacagc ctgacggacc gcgacataga catgcgccgg
    12901 atgcacggcg attttgccaa tatctgcgcc atcaagcccg atatctcctg tcagtgcacc
    12961 taatacatca cccgggcgca ttttggcttt tttcccgcca tcgatacaca acgttgccat
    13021 ttctgcttcc agcgtcacaa tggaactatt agctggcggc gtttgccagt taagttttat
    13081 ctgcaacatg tcagaaatga tattggcccg ctgtgcttct tccggagcac agaaactgat
    13141 cgccagaccg ctatttcctg cacgagctgt acgaccgatg cgatgtacat gaacttcagg
    13201 gtcccacgcc agctcaaagt tcaccaccag ctcaagcgat ttaatatcca gaccacgcgc
    13261 agcaacatca gtcgcaacca gtacacgggc gctaccgtta gcaaaacgta ccagggtctg
    13321 atcgcgttgc tccagatcgc cgtgtaacga caatgcactt tgctctactt cattcagcgc
    13381 gtcgcagaca gcctggcaat cttttttggt attgcaaaac accacacaag aggatggctg
    13441 atgcaagctt aataaccgtt gcaacagagg aattttgcct ttactggatg tctcataaaa
    13501 ttgttgttca atgggtggca aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg
    13561 ttgcactcgt ccgctgattg cggcgatggc ttccggccag gttgccgaaa acagaagcgt
    13621 ctgtcgagat gcaggcgcaa aacggatgac atcatcaatg gcatcgctaa atcccatatc
    13681 cagcatgcgg tcggcctcat ccatcaccaa agtattcagc gcatccagtg ataccgtgcc
    13741 tttttgcaag tgatccagca aacgacccgg cgttgccacg ataatatgcg gcgcatgttg
    13801 caacgaatca cgctgcatac cgaacggttg accaccgcac aacgtcaaaa ttttggtatt
    13861 tggcagaaaa cgcgccagcc gacgcaattc acctgccacc tgatccgcca gttcacgcgt
    13921 aggacacagc actaaagcca gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc
    13981 gaggccaaaa gccgccgttt tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc
    14041 tgcaaggatc gccggaagcg cggcggcctg caccggcgtc atggttaaat aacccaactc
    14101 attaaggttc gtgagttggg cgggaggcaa aacattcagg gtagaaaaag cggtcacaat
    14161 ctattctcgt ggtcatcgac gcaaagttag caggcgcgta tcctcgcaga tctacgctca
    14221 cgatgcgaca atttaatcgg ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga
    14281 gggatcggat cgggcactgg aacaggatcg ccaggaatcg gttcagggac aggaatttgc
    14341 aaataaataa gtgtcgtcat atttccctcc ggtcattggg tggactctta aagggtagac
    14401 gctgataaat aacaggcaaa aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat
    14461 tgtgctatgc agtaattcaa aaaaggaagt aagacaatat ggagcgcaac gcccatcgct
    14521 tgacgttgca ttcacctgca agggagatat tgccctgaat gggtagagag tttattgact
    14581 tcgctcaaac tttgcggcgt ttttgtatac agacagccga aaaaattgct tttgttacaa
    14641 ccatttacta cggtgcaacc ataaagcaac accaccaata agaacaacta acagaataca
    14701 aaaaattgaa aatccgaatt gccacccgcc gccagggatc ccaccaaggt tgacgccaaa
    14761 taacccggtc agaaaggtac tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt
    14821 acgacgagct aaattttcct gcatcacctg agcgatttca tccgccatca cgccagtccg
    14881 tgctatacag gcgtcgattt cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat
    14941 ccgacggcgt tggtcatcgc tcatccacgg caaacgttca ctggcaagac gagcataaac
    15001 atcacgttgc ggtgccatat agcgacgcat cacaattaat tgtttgcgca gcagagccag
    15061 gaatccacgc ggtggaattt gctgatcaag gagattatct tcaaggtcga taattttatc
    15121 gtgcagctgc tcgataaatt cactggaatg atcggtcaac gcatcgcaca catccaccag
    15181 ccatcccccg caatcggtcg gacccgtgcc ctcttccaga tcgctcacca catcgtccag
    15241 cgccagcact ttgcgttgtc gggtcgaaac aattaaccgc ccgtccatat atacacgcat
    15301 ggcgaccagt tgatcggggc gttcatcggt gctgccgttt atacagcgca atgtaatcag
    15361 cgtgccttca ccgaaacggc tgactcgggg ccgtgtgctc tcgcccgcca gcgcatcacg
    15421 tacgttattg ggaagcagcg gtgttgtcgc cagccattgg gcgctatcat ggtgtacata
    15481 attaaggtgg agccagcagg gatgcgcttc atcaatcaca tctgtatttt ccagcggttt
    15541 aacgccgcct ctaccatcca gcatccaggc aaatactgca tccgggacat taacgtccga
    15601 tcccttaatc gcttccacag tgcctccatc atcaacgcat tattttgtag tctagccttc
    15661 tggccctgtt acgcaacatc tcatcacccc attaccctga aatgattaat aaaattctgt
    15721 ctaaattgaa tacaaaaagc aaaatgcttt tccgtataca aaccgtgtga agtgttaaat
    15781 agcgtctatc attatcagaa ttatctgatc atatgacgtg gcttttttgc gatcggatag
    15841 caacaaaaat tgataaaaat aacgggatct caatgattac gcacaacttc aataccctgg
    15901 acttactcac cagtcctgtc tggattgttt cgccctttga ggaacagtta atttatgcca
    15961 atagcgcggc gcgactgttg atgcaagacc tcacgtttag tcagctacga accggaccct
    16021 attccgtctc ctcacaaaaa gaactgccga aatacctctc cgatctgcaa aaccaacacg
    16081 atattatcga aatcctcact gttcagcgta aagaagagga aacagcatta agctgtcggc
    16141 ttgttttgcg agagctgaca gaaacagaac cggtgattat tttcgaaggt atcgaagcgc
    16201 cggcaacgct gggtttaaaa gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt
    16261 tttatgcgcg cttttttctg actaactctg caccaatgtt gttgattgac ccgtcacgag
    16321 atggacaaat cgtcgatgct aacctcgccg cgctcaattt ctatggttat aaccatgaaa
    16381 cgatgtgcca gaaacatacc taggaaataa atatgcttgg gcgtcgcgtc atgcctatca
    16441 tgcatgaaat ttcgcattta cccggcggtc ataacccttt gaattttgtt cataaactgg
    16501 cggatggttc tactcgtcat gtgcagacct atgccggacc gattgaaatt tatggcgaca
    16561 agctcatgtt atgtattgtg catgatatta ctgagcaaaa acggctggag gagcagctgg
    16621 aacatgctgc tcaccatgac gcgatgaccg gattactgaa tcggcgacag ttttatcaca
    16681 ttacggaacc aggccaaatg cagcatctcg ccatcgctca ggattacagc ttgttgctca
    16741 tcgacaccga tcgttttaaa cacattaacg atctctatgg gcattctaaa ggtgatgagg
    16801 tgttatgcgc cctcgcccgc cccctcgaaa gttgcgctcg caaaggcgat ttggtgtttc
    16861 gttggggagg cgaagagttt gtcttattgc taccaagaac cccactggat accgcgcttt
    16921 cgctggctga aactatccgc gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct
    16981 ttaccgtcag cattggtgtg gcgcatcacg aaggaaatga aagcatcgat gaactgttta
    17041 aacgcgttga tgatgctttg tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg
    17101 cataagccgc ggatgcgtct cgagatcaac gactgcgctt agcgtggcgc tcccagtttt
    17161 cttgcttcgc ctgcgccgtt ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat
    17221 gcggtagcgc ggtgcaatat gcctgaacat catcaaattc ggtcagccag cgcgccacat
    17281 agctgcggac aatattggca tgtgatttat cttcccgccc tttaccatga ataatcagca
    17341 cattacgcaa accatccgcc agggcttgtt gaatgaaact gaacaccatt ttgcggcact
    17401 cttccaccgg ctggcgcaaa agattcaggc tcgcctgttg cggatattta ccactgcgca
    17461 gcttatccag caccccatgc tgcaaccctt cctgccgaaa ctccagcggc tgacttagcg
    17521 ggatgatgtc gagaaatccg gtagtgagga aattatcaag ctgcagcgtg tcgatacgct
    17581 gcggcgcacg ttggttacgc gttggatgcc agtggacatc tgtagcacgt ttcagcggct
    17641 ggacatcttc catggcgtca agaaacagcg atttgtcgtc aaggttcatg taacctccta
    17701 tgcaccttca cacgtcgtga ttcagacggg ttaattgttc atacttactg catcgttccc
    17761 ttcacagatt gaaacaatac tctacttcat acctgtgagt atctgcgcag gcggggcatt
    17821 ttatactagc ctgttagcag tatcgaacag tttagcccga gtttaattca gcacaggctc
    17881 cgatcaggat gatttattca tagcaggaac caacaaaaat gcgataaata acagaccact
    17941 gattagcaga aaggtaccga aaatgccata gtttgcgcct ccctcagcat caattgactc
    18001 aataatttgg ttgaagccag tatttccgat gtctggtcac ttctactgcg attaaaaaat
    18061 aaagcacttc tggaaatgaa gtcattcacc gtatggggaa cagaaaccat tttttcaccc
    18121 attctcgaaa aatgaaaatt agttcgatta ttgagaatag agccacacag ggcaatacca
    18181 atcgcgcccc caagattacg tgttaaatta aatacaccag aggctaactt cagccgttct
    18241 tttggaattc cccccagcgt taatgttacg atgggggcca tcgcaaactg ctgcaaaatc
    18301 cctctaattg cctgtggaaa cagtaattcc tgccatcccc attcgtgggt tataggggta
    18361 aaaagataca ttgaaaacac aaatccgccc agccccgcca tcaacagcca tcgcagattt
    18421 atttttttac ttaaccagaa gtaaaaggga acagaaaata actggaaaat acctgttgtg
    18481 caaacggcaa aacctatctc ttcggcattt aatccgcgaa tctgccccaa aaacacaggt
    18541 attaaatata cagtagagaa tatcccaacc cctccagaaa aagaaaaaaa acatcccaga
    18601 gtgaaatttt tatccttaaa agcatgaaga tccattatcg gattggatat tgtaagtgta
    18661 cgcgcagcaa ataaaataaa actcaccaat gccagaactg atgtcagcaa aatagtatta
    18721 tcatccagcc atccccagcg cgctccttct tcaagggtat attccagaca tcccagagtc
    18781 atggccaaca ggataatact cggataatca gcaactttca gtaaagagag atcaggttta
    18841 tcaaaattaa ccagaaaagg aatactcaaa actaaatata tgccaggtaa tatattgata
    18901 taaaatagcc atcgccagtc gaggttatca gtgagccagc cccccagagt tggccctagc
    18961 gtcggtgaca gtgaggccag cgcactgact accgcagcag ccaaccccaa ctctttaccc
    19021 tggtaataaa taaacgccgt cgtgaaaact aaagggatca tggatgcgcc agcagctccc
    19081 tgtaaagcac gaaacaaaat catgatctga atattccatg caagtccaca cgcgatactc
    19141 attaaagtaa atattcccgc ggatagagta aaaagccaac gagtggaaaa tacccgtgac
    19201 agccacccag agagcggaat aacaataatc tcagcaatta aatagctggt ctgaagccag
    19261 gccatttcgt ctttacctgc ggacagacca ccacctattt catttaggga tgacgataca
    19321 atttgaatat caagctgagc aataaataat cccatacaca tactggcgaa agcaatatgt
    19381 ttacgcatag acatcatctc tcatcaacac tgacggtgac ggacagtccc gggcgaagaa
    19441 gttgctttaa ttcctttgcg tcgtcaaatg caatccgtac cgggacacgc tgaacaattt
    19501 tggtgaaatt gccagtggca ttttctatcg gaatcaagct gaaactggct cctgtcgctg
    19561 gggaaaggct ttcaatgtga ccgtgaaaca cttccccttt gagaatatca gcacggatct
    19621 cagccttcat tcctggcttc attcccgcta tttggttttc ttta
//
LOCUS       DAJVQH010000073        19629 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJVQH010000073 DAJVQH010000000
VERSION     DAJVQH010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.073"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..1224
                     /locus_tag="OQM87_002575"
     CDS             <1..1224
                     /locus_tag="OQM87_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1508663.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTRKAALLGSEHKRVPDDLENH
                     PVITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="OQM87_002576"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="OQM87_002576"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1508664.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="OQM87_002577"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="OQM87_002577"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCS1508665.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="OQM87_002578"
     CDS             2714..3427
                     /locus_tag="OQM87_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCS1508666.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="OQM87_002579"
     CDS             complement(3399..4076)
                     /locus_tag="OQM87_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1508667.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="OQM87_002580"
     CDS             complement(4070..4747)
                     /locus_tag="OQM87_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCS1508668.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTCSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="OQM87_002581"
     CDS             complement(4735..5118)
                     /locus_tag="OQM87_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCS1508669.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNELAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="OQM87_002582"
     CDS             complement(5134..5442)
                     /locus_tag="OQM87_002582"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508670.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="OQM87_002583"
     CDS             complement(5443..6021)
                     /locus_tag="OQM87_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCS1508671.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="OQM87_002584"
     CDS             complement(6044..6475)
                     /locus_tag="OQM87_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1508672.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELEFSRQPCALSGYDELHISPVK"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="OQM87_002585"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="OQM87_002585"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCS1508673.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHSEAHIAAGAKKVLFSHPGSNDLGATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="OQM87_002586"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="OQM87_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCS1508674.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="OQM87_002587"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="OQM87_002587"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCS1508675.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="OQM87_002588"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="OQM87_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCS1508676.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="OQM87_002589"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="OQM87_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCS1508677.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="OQM87_002590"
     CDS             12291..13031
                     /locus_tag="OQM87_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCS1508678.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="OQM87_002591"
                     /pseudo
     CDS             13198..14148
                     /locus_tag="OQM87_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OQM87_002592"
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OQM87_002592"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCS1508679.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OQM87_002593"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OQM87_002593"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCS1508680.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OQM87_002594"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OQM87_002594"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCS1508681.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OQM87_002595"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OQM87_002595"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCS1508682.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLGKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKMDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMTEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRAARDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVAGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
BASE COUNT         4714 a         4909 c         5276 g         4730 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cccggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg ggattattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggccgcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tctgcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgactctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa accgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcagacgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggtgctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agtcgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ctatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggcgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgaccacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct acaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagctcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaactcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc caggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgggta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagcca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctagat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat agcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcggcg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ttggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcacattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgtgattg ggctttctcg tttctcgccg ttataaagag
    13741 cgtcaagagc aagagctgct tcaggagctc ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggtgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgcg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagcc
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgtaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttattgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgacgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccgctacg tgaacgtcgt tttctacggc caggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggc ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcggtca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tccattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttaccc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgcc
//
LOCUS       DAJVQH010000074        19550 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJVQH010000074 DAJVQH010000000
VERSION     DAJVQH010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.074"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(47..139)
                     /locus_tag="OQM87_002596"
     tRNA            complement(47..139)
                     /locus_tag="OQM87_002596"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:Ser,seq:gct)
     gene            complement(455..640)
                     /gene="csrA"
                     /locus_tag="OQM87_002597"
     CDS             complement(455..640)
                     /gene="csrA"
                     /locus_tag="OQM87_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCS1508683.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(875..3505)
                     /gene="alaS"
                     /locus_tag="OQM87_002598"
     CDS             complement(875..3505)
                     /gene="alaS"
                     /locus_tag="OQM87_002598"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCS1508684.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3633..4133)
                     /gene="recX"
                     /locus_tag="OQM87_002599"
     CDS             complement(3633..4133)
                     /gene="recX"
                     /locus_tag="OQM87_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCS1508685.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4376..5437)
                     /gene="recA"
                     /locus_tag="OQM87_002600"
     CDS             complement(4376..5437)
                     /gene="recA"
                     /locus_tag="OQM87_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCS1508686.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5517..6014)
                     /gene="pncC"
                     /locus_tag="OQM87_002601"
     CDS             complement(5517..6014)
                     /gene="pncC"
                     /locus_tag="OQM87_002601"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCS1508687.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(6159..7244)
                     /gene="mltB"
                     /locus_tag="OQM87_002602"
     CDS             complement(6159..7244)
                     /gene="mltB"
                     /locus_tag="OQM87_002602"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCS1508688.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7500..8063
                     /gene="srlA"
                     /locus_tag="OQM87_002603"
                     /gene_synonym="gutA"
     CDS             7500..8063
                     /gene="srlA"
                     /locus_tag="OQM87_002603"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCS1508689.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8060..9019
                     /gene="srlE"
                     /locus_tag="OQM87_002604"
                     /gene_synonym="gutE"
     CDS             8060..9019
                     /gene="srlE"
                     /locus_tag="OQM87_002604"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCS1508690.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9030..9401
                     /gene="srlB"
                     /locus_tag="OQM87_002605"
                     /gene_synonym="gutB"
     CDS             9030..9401
                     /gene="srlB"
                     /locus_tag="OQM87_002605"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCS1508691.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9405..10184
                     /gene="srlD"
                     /locus_tag="OQM87_002606"
     CDS             9405..10184
                     /gene="srlD"
                     /locus_tag="OQM87_002606"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCS1508692.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10289..10648
                     /gene="gutM"
                     /locus_tag="OQM87_002607"
     CDS             10289..10648
                     /gene="gutM"
                     /locus_tag="OQM87_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCS1508693.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10715..11488
                     /gene="srlR"
                     /locus_tag="OQM87_002608"
                     /gene_synonym="gutR"
     CDS             10715..11488
                     /gene="srlR"
                     /locus_tag="OQM87_002608"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCS1508694.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11481..12446
                     /gene="gutQ"
                     /locus_tag="OQM87_002609"
     CDS             11481..12446
                     /gene="gutQ"
                     /locus_tag="OQM87_002609"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCS1508695.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSSTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12443..13957)
                     /gene="norR"
                     /locus_tag="OQM87_002610"
     CDS             complement(12443..13957)
                     /gene="norR"
                     /locus_tag="OQM87_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCS1508696.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGNDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14144..15583
                     /gene="norV"
                     /locus_tag="OQM87_002611"
     CDS             14144..15583
                     /gene="norV"
                     /locus_tag="OQM87_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCS1508697.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15580..16713
                     /gene="norW"
                     /locus_tag="OQM87_002612"
     CDS             15580..16713
                     /gene="norW"
                     /locus_tag="OQM87_002612"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCS1508698.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16841..19093)
                     /gene="hypF"
                     /locus_tag="OQM87_002613"
     CDS             complement(16841..19093)
                     /gene="hypF"
                     /locus_tag="OQM87_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCS1508699.1"
                     /translation="MAKNTFCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19246..>19550)
                     /locus_tag="OQM87_002614"
     CDS             complement(19246..>19550)
                     /locus_tag="OQM87_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1508700.1"
                     /translation="SCDKGFVHVMQERCIGCKTCVVACPYGAMEVVVRPVIRNSGAGL
                     NVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKLEQLSAEKRRRTALMF"
BASE COUNT         4587 a         5450 c         4989 g         4524 t
ORIGIN      
        1 tcggttcgta gccgagtact ctatccagct gagctacgga tgcaaatggc ggtgaggcgg
       61 ggattcgaac cccggatgca gcttttgacc gcatactccc ttagcagggg agcgccttca
      121 gcctctcggc cacctcacca cacgcctctt acgagtgctt cgaagaactt gtttatcgct
      181 catcgtcgct gcgtggcgca catattactt tctgagactt ataagtcaaa caatttttcc
      241 cacactttta tcgtttgcac acttcacgct caattagtct gtaaaaacgt caaaaagagt
      301 gttttatcta cagaaaaacg gaggtctggc agatagcagt aatgcaaaaa aatggagact
      361 taagttgaat gaacgggagt aaagcgaaaa gactatagag tgaaggagaa attttgaggg
      421 tgcgtctcac cgataaagat gagacgcgga aagattagta actggactgc tgggattttt
      481 cagcctggat acgctggtag atctcttcac ggtgaacaga aacttccttc ggggcattta
      541 cgccaatacg tacctggttg ccctttaccc ctaaaactgt cacggtgacc tcatccccaa
      601 tcatgagggt ctcaccaact cgacgagtca gaatcagcat tctttgctcc ttgaaagatt
      661 aaaagagtcg ggtctctctg tatcccggca ttatccatca tataacgcca aaaagtaagc
      721 aacgacaaac acattacatg taagcagtca tggcattaca ttctgttaaa cctaagttta
      781 gccgatatac acaacttcaa cctgacttta tcgttgtcga tagcgttaat gcgaatgccg
      841 tgaagcgagt ccacggcatt gcctgacgct tatattattg caatttcgcg ctgacccagc
      901 ctttcacact ggctaacgct gcaggtaagg ccgcagcatc cgtaccaccg gcttgcgcca
      961 tgtcaggacg tccaccaccc ttgccgccca cctgctgagc gaccatacca atcagttccc
     1021 ctgctttcac acgatctgtg acgtccttag atacgcctgc aatcagagaa accttacctt
     1081 cggctaccgt tgccagcacg ataattgtcg accccagctg attttttaaa tcgtcaacca
     1141 tggtacgcaa cattttcggc tcaacaccgc taagctcgct aaccaacagc ttaacaccat
     1201 taacatcaat tgccttactg gaaagatttg cgctctcctg tgcggcagct tgttctttaa
     1261 gctgttgtag ttctttttct aactgacgcg tacgttccag tactgagcgc actttatccg
     1321 ccagattatt gctatcgcct ttcagcagat gcgcgacttc gcttaagcga tcgctgtctg
     1381 catgaacggt ggtgatagcg ccttctccgg ttaccgcttc gatacgacga acgcctgcag
     1441 cagtacccga ttcagagatg atgcggaaca gaccaatatc accagtgcgg ctggcgtgag
     1501 taccgccaca cagctcggtg gagaaatcgc ccatgctcag cacgcgtaca cgctcatcat
     1561 acttctcgcc gaacagcgcc atcgcacctt tcgctttcgc cgcttcgaga ttcatgatgt
     1621 tggtttcgat cggcaaattg cgacgaatct gtgcgttcac caggtcttcg accgcacgaa
     1681 tctcttccgg tttcatcgct tcgttgtgtg agaagtcgaa gcgcagcacc ttgtcgttaa
     1741 ccagtgaacc tttctgcgat acatgagtcc ccagaacctg gcgcagcgca gcgtgcatca
     1801 ggtgcgttgc ggagtgattc agacgaatac gggcgcgacg agcctcatca atatcagcct
     1861 gcacagcgtc gcccactttc agagaacccg cagcaagttt accgatgtga ccaatcgcct
     1921 ggccgtattt ctgcgtatct tccaccgcga aggagaagtt agcgcctttc agttcgcctt
     1981 tatcaccaac ctgaccgccg gattccgcat agaatggcgt ttgatccagc acgaccacag
     2041 cttcctggcc tgcattgatg gcatcaaccg ctttaccatc aacaaacagc gcggtcactt
     2101 tgccgttcag ttccagatgg tcatagcctt taaattcaga tgcactatca acacggatca
     2161 ttgcgttgta atcggcacca aagccgctgg cttcgcgcgc gcgacgacgc tgctcttcca
     2221 ttgctgcttc aaaaccagct tcgtcaactt tgatgttgcg ctcacgacaa acatcagccg
     2281 tcaggtcaac cgggaagcca taggtgtcgt acagacggaa agccgtttca ccatccagcg
     2341 tatcaccaga aagttttgcc agctcttcat ccagcaacgc cagaccgcgc tccagagtac
     2401 gggcaaactg ctcttcttca gtcttcagca cctgctcaac ctgcgcctgc tggcgtttca
     2461 ggtcttcacc cgcagagccc ataacgtcga tcagcggacc aaccagtttg tagaagaagg
     2521 tttctttcgc gccgagcata ttgccgtgac gcactgcgcg acgaatgata cgacgcagta
     2581 cataaccacg gttttcattg gacggcatta cgccatccgc gatcaggaac gcacaagaac
     2641 gaatgtggtc agcgattacg cgcagcgatt tattgctcag atcggtcgcg ccagtgactt
     2701 tcgctaccgc ctggatcaac gtgcggaaca ggtcgatgtc atagttagag ttaacgtgtt
     2761 gcagcaccgc agcaatacgc tccagaccca taccggtatc tacagacggc ttcggcagcg
     2821 gttccatcgt gccatctgcc tggcggttga actgcatgaa gacgatgttc cagatctcaa
     2881 tgtagcggtc gccgtcttct tccggacttc ccggagggcc accccaaatg tggtcgccgt
     2941 gatcgtagaa gatttcggtg cacgggccgc acggaccagt gtcacccatc tgccagaagt
     3001 tgtcagatgc gtatggcgca cctttgttat cgccgatgcg aataatacgt tcgcgcggga
     3061 tcccgacttc tttttcccag atttcgtagg cttcgtcgtc gctttcatag acggtaaccc
     3121 acagacgctc tttcggcagg gcaaaccatt tttcgctggt cagcagttcc catgcaaact
     3181 gaatggcatc gtgtttgaaa tagtcgccga agctgaagtt gcccagcatt tcgaagaagg
     3241 tatggtgacg cgcggtgtaa ccgacgtttt ccaggtcgtt gtgtttacca cccgcacgca
     3301 cgcagcgttg ggaagtggta gcgcgggaat aattacgctt gtcgagccca aggaacacat
     3361 ccttgaactg gttcatcccg gcgttggtaa acaacaaagt tgggtcgtta tggggtacca
     3421 gggagctgct ggcaactacc tgatgtccct tactatggaa aaagtcgaga aacgcctgac
     3481 ggatctcagc ggtgctcttg ctcataatta tcctgaaatc aagctaacga aatatcgcca
     3541 ccagctccag cgtgtcttaa ccgccgggct ggtaactgaa aagtgggaat aagataagtt
     3601 ttcttgactg ggaagtaaaa taccgtatgc gttcagtcgg caaaatttcg ccaaatatcc
     3661 tggatatctt ccatcagata gccacgatag agcagaaaac gctggatctt aactttttct
     3721 gaaaagacag ttggcaaagg ttcaccatat tttcgcgtcg cctgatcgcg cgccagtgcg
     3781 caccagtcga tgtcacattc acgcatcgct ttttctgtcg cttcgcggga aatacctttc
     3841 tgattcagtt cctgacgaat acgtgcaggt ccataacctt tgcggctacg gctggcgata
     3901 aagcgcgcaa caaatcggct gtcatcgaga tagccatgtt catggcacca ggcaataacg
     3961 cgctcgtaat cttctgccgt agcatcaatc tcttctgggc catttttgcc cataatcggt
     4021 gccgcgagtt tacgtcgcag ttcttgctca ctgtgatcgc gcaccgccag aatgcgtacc
     4081 gcacgatcca acaggcgagc atatgccggg cgacgggatg ttgattctgt catggcatat
     4141 ccttacaact taaaaaagca aaagggccgc agatgcgacc caggttttgc tgataaacag
     4201 atttaatagt caatgagtat gcagaatgtt cccgcaggcc caattcgtta gaggatcgag
     4261 gtgagagtaa tttttcacgc cagtgctggc ctgttcccct caccctaacc ctctccccaa
     4321 aggggcgagg ggaccgtccg tgcacacatt atctattatc atcaataacg aacgattaaa
     4381 aatcttcgtt agtttctgct acgccttcgc tgtcatctac agagaaatcc ggcgttgagt
     4441 tcgggttgct cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt
     4501 tatctttcag ccaggcagtt gcattcgctt taccctgacc gatcttctca cctttgtagc
     4561 tgtaccacgc gcctgctttc tcgatcagct tctcttttac gcccaggtca accagttcgc
     4621 cgtagaagtt gataccttcg ccgtagagga tctgaaattc agcctgttta aacggtgcag
     4681 cgattttgtt cttcaccact ttcacgcggg tttcgctacc caccacgttt tcgccctctt
     4741 tcaccgcgcc gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac
     4801 caccggtagt ggtttccggg ttaccgaaca tcacaccaat tttcatacgg atctggttga
     4861 tgaagatcag cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc
     4921 tcatcatacg tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt
     4981 tcggcgtcag tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca
     5041 gggcgtcaca gatttccagt gcctgctcgc cggtatccgg ctgggagcac agcaggttat
     5101 cgatatcgac gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa
     5161 acgcacaggt tttaccttca cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt
     5221 taccggaaga ttccggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc
     5281 caagcgcgat atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt
     5341 cttcacccag gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg
     5401 ccgccaacgc tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta
     5461 ataccggata gtcaatatgt tctgttgaaa caattatact gtatgctcat acagtatcaa
     5521 gtgttttgta gaaattgttg ccacaaggtc tgcaatacat acgcagtagc ctgacgacgc
     5581 accgcatcac ggtcgccgct gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg
     5641 gcaaaagcaa accagacggt gccgacaggc ttctcttcac tgccgccatc cggcccggcg
     5701 ataccactaa tagacacggc ataatcagca cgagccgctt tcagtgcacc tatcgccatt
     5761 tccaccacga cgggttcact caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg
     5821 atcatctgcg ctttggcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag
     5881 ctaccggcaa tatcggtaat cactttcgct acccaaccac cggtacaaga ctcggcagtt
     5941 gttacggttg cgccacgggc tttcagcgcc tgcccaacct gttcgcttaa ctgcatcagt
     6001 tcactgtcag tcatcacaac ctctgtcagt caaaaattta tgccggacaa gatagcactt
     6061 tcgtcaggaa gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat
     6121 tagccagagg gaagctcacg cccccctctt gtaaatagct actgtactcg cgccagcgcc
     6181 acggcttgtc ctaactgcca gaccgccatt gcgtaatggg tgctgtggtt gtaacgggtg
     6241 atggtgtaga agttcggcag accgtaccag tactggtagc cggtgccaac atccagacgc
     6301 agcaggctgg cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgcggcggca
     6361 agctgcgaaa tgctgtactt agttttgaaa ccatttggca agcctggagc ctgaccgttt
     6421 gccattatcg cgacctgatc gcctttcacc cagccgtgcg ctttgaaata gttcgccacg
     6481 ctaccgatcg catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc
     6541 gcatattgtt tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa
     6601 cctttcagat tgagcggatc gtcctgctcg tcgcgcgcca tcagcaggaa ggtttccagc
     6661 tcgccagaga aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg
     6721 aggatgcgag ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg
     6781 ataatttccg gcggtacgcc atacacctgc cacgcgcgat tcaacgcatc ttcatactga
     6841 ttccagaaaa ccacaccgtt ctgcacgttg tccggcgtaa taaatttttt gcgataacgc
     6901 agccatgcgc cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc
     6961 atcagccgca gtaccgaatc cagacgcttc gcctgggaga gaatttcctg caactgctga
     7021 cgatcgaaac cgtgtttgtt caccattttg tcgatgaact gctgggcatt cgggttatta
     7081 gcgaaatcgc cgcccatctg catcacattg tgctgcggct caagcaggaa gccgccagac
     7141 ggcgttccgg tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc
     7201 aacacaaaaa ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac
     7261 agattcaacc aggggcaagt atggtaaagc atcacgcccc gcacaaggaa gcggtagtca
     7321 ctgcccgata cggactttac ataactcaac tcattcccct cgctatactt ttactcaaac
     7381 tttcaaatta aaatatttat ctttcatttt gcgatcaaaa taacactttt aaacctttca
     7441 acctgattag attaggttgc cgtttggtaa taaaacaata aatcctgaag gagagaacaa
     7501 tgatagaaac cattactcat ggtgcagagt ggtttatcgg gctgttccaa aagggcggag
     7561 aggtgtttac cggaatggtg accggcattc ttccgctgtt gattagcctg ctggttatca
     7621 tgaacgcact gattaatttt atcggtcagc atcgtattga acgttttgct caacgttgcg
     7681 ccggtaaccc tgtttcccgt tacctactgt taccgtgcat tggcacgttt gtcttttgca
     7741 atccgatgac cttaagccta ggtcgcttta tgccggaaaa gtacaaaccc agctactacg
     7801 cggcggcctc ttatagctgc cactcaatga atggcctctt cccccatatc aaccctggcg
     7861 aactgtttgt ttatcttggc attgccagcg gtctgacaac gctgaacctg ccacttggcc
     7921 cactggcggt gagttatctg ctggttggtc tggtcaccaa tttcttccgc ggctgggtga
     7981 ccgatctgac caccgccatt tttgagaaaa agatgggcat tcaacttgaa caaaaagttc
     8041 acctggcagg agcaacatca tgacgcgtat tcggatcgaa aaaggaacgg gtggctgggg
     8101 cggcccgctt gagctggaag ctacgccggg caaaaaaatc gtctatatca ccgccggtac
     8161 ccggcctgcg attgttgaca aactggcaca acttactggc tggcaggcta ttgacggatt
     8221 taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac
     8281 attacgctgc ggcatctatc cgaaacgacg tattcccacc attaatatcc actcgacggg
     8341 caagtccggt ccgctggcgc agtacattgt ggaagatatt tatgtctctg gcgtaaaaga
     8401 agaaaacatc actgtagtgg gtgatgcgac accacaaccc tcttccgtgg gccgtgacta
     8461 tgacaccagt aagaaaatca ccgaacaaag cgatggttta ctggcgaagg tgggaatggg
     8521 catggggtcc gccgttgccg tgttgtttca gtctggtcgt gacaccatcg acactgtatt
     8581 aaaaaccatt ctgccgttta tggcattcgt ctcggcgctc attggcatca ttatggcttc
     8641 tggccttggt gactggattg cccacggtct tgctccgctg gcgagccatc cactgggtct
     8701 ggtcatgctg gcgctcatct gctccttccc actgctttca cctttcctcg gcccaggcgc
     8761 agttatcgca caggttatcg gcgtattgat tggcgtgcag attggtctcg gcaatattcc
     8821 gccgcatctg gctttaccgg cactgtttgc catcaacgcg caggcggcct gcgacttcat
     8881 cccggtcggt ttgtcgctgg cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc
     8941 tgtactggtg agccgctttt taaccggcgc gccgactgta ctgatcgcct ggtttgtctc
     9001 cggttttatc tatcaataga ggctgaaaca tgaccgttat ttatcagacc accatcaccc
     9061 gtatcggcgc gagtgccact gacgccctca gcgaccagat gctcatcacc tttcgtgaag
     9121 gcgcgccagc ggacctcgaa gagtattgct tcattcattg ccacggcgag ttgaaaggtg
     9181 cactccatcc cggtttgcaa ttttcactcg ggcagcatcg ctatccggtg accgctgttg
     9241 gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt
     9301 taaacgaagc ggaatttccg ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg
     9361 cgccgggatc ggttttgaag tttgaatctg ttaaggagta aaaaatgaat caggttgccg
     9421 ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg ccacggtctg gctgccgagg
     9481 ggtatcgcgt cgcggttgtc gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa
     9541 ttaacgccga atatggtgaa ggtatggcgt acggttttgg tgctgacgcc actagcgagc
     9601 aaagcgttct ggcgctctct cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg
     9661 tctatagcgc cggaatagca aaagcagcct ttatcagcga cttccagctc ggcgattttg
     9721 accgttcgct acaagtgaat ctggtgggtt atttcctgtg tgcccgtgaa ttttcgcgtt
     9781 tgatgatccg cgacgggatt cagggtcgca ttattcagat caactcgaaa tccggcaaag
     9841 tgggcagcaa acacaactct ggctacagcg cagcgaaatt tggtggcgtc gggctgactc
     9901 aatcactggc gctggatctg gcggagtacg gcattacggt gcattcactg atgctcggta
     9961 acctgctgaa atcgccgatg ttccagtcac tgttgccaca atacgcgacc aagctgggta
    10021 tcaaaccgga tcaagtcgag cagtattaca tcgacaaagt accgctcaaa cgcggctgcg
    10081 attatcaaga tgtgctgaat atgctgctgt tctacgccag tcctaaggcg tcgtactgca
    10141 ccggacagtc gatcaatgtc accggcggtc aggtgatgtt ctgatcaaca gcggagatcc
    10201 gttaaggatc tccgtgagac tatagaatgc ctgatgcgct acgctcatca ggcatacagg
    10261 acttccgcca ctacattaag gaaaagttat ggtatccgca ctcatcaccg tcgccgttat
    10321 cgcctggtgt gcgcaactgg ccttaggcgg ctggcaaatt tctcgtttta accgtgcctt
    10381 cgacacacta tgccagcaag ggcgggttgg cgtgggccgt tccagcgggc actttaaacc
    10441 gcgggtcgtg gtcgccatcg cgctggacga tcagcagtgc atcgtcgaca ccttgtttat
    10501 gaaaggactg actgtcttcg cccgaccgca aaaaattccc gcaattaccg gcatgcatgc
    10561 gggtgattta cagcccgatg tgatctttcc ccatgatcca ctatcacaga atgctctatc
    10621 attggcgctt aaactgaaac gtggataatt tcgttgtgaa tgttacttgc ttgcgaagtt
    10681 atcattttga aacctaaatc aggtaatcac gcccatgaaa cctcgtcagc gtcaggccgc
    10741 cattctggag tatctgcaaa agcagggtaa atgctcggtt gaagaattgg cgcaatactt
    10801 tgacaccaca ggcacaacca ttcgcaaaga tctggtcatt ctggaacatg ccggaaccgt
    10861 cattcgtact tatggcggag tggtgttgaa taaagaggaa tccgatccgc ctatcgatca
    10921 taaaacgctc atcaacaccc acaagaaaga gctgattgca gaagctgccg ttagttttat
    10981 ccatgatggc gattcgatca ttcttgatgc tggcagtacc gttttgcaga tggttcccct
    11041 gctctcgcgc tttaataaca tcacggtgat gaccaacagc ctgcatatcg tcaatgcgct
    11101 atccgaactg gataacgaac aaactatcct gatgccaggc ggaacatttc gcaaaaaatc
    11161 ggcctcattt cacgggcaac tggcagagaa tgccttcgag catttcacct tcgataaatt
    11221 gtttatgggc accgacggca tcgatctcaa tgcgggcgta accacgttca acgaggttta
    11281 taccgtcagt aaggcaatgt gcaatgccgc gcgcgaagtg attttgatgg cggactcatc
    11341 aaagtttggc cgtaaaagcc ccaacgtagt ttgcagtctt gaaagcgtcg ataagctaat
    11401 taccgacgca ggtatcgatc cggcgtttcg tcaggcgctg gaagagaaag ggatcgatgt
    11461 gatcataacc ggagagagca atgagtgaag cactactgaa cgcgggacgt cagacgttaa
    11521 tgctggagtt gcaggaagca agccgtttac cggaacgtct gggcgatgat tttgttcgcg
    11581 ccgccaatat catcctgcac tgcgaaggca aagtggtggt ttcgggaatt ggcaaatcgg
    11641 gccacattgg taagaaaatc gccgcaacac ttgccagtac cggcacaccg gctttttttg
    11701 tccatccggc agaagcactg cacggcgatc tggggatgat tgaaagccgc gatgtgatgc
    11761 tgtttatctc ttactccggt ggcgcgaagg aactggatct gattattccg cgtctggaag
    11821 ataaatctat cgcgctgctg gcgatgaccg gcaaaccgac gtcaccgctg ggcctggcgg
    11881 cgaaagcggt gctggatatc tccgtagaac gcgaagcctg cccgatgcac cttgcgccga
    11941 cctccagcac cgtcaatacc ctgatgatgg gtgacgcgct ggcgatggcg gtcatgcagg
    12001 cgcgcggttt taatgaagaa gattttgccc gctcccaccc agctggggca ctgggcgctc
    12061 gcttgctgaa taaagtgcat catctgatgc gtcgtgatga cgctatccca caggtagcgt
    12121 taaccgccag cgtgatggat gcgatgctgg aactcagcag caccggtctg gggctggtgg
    12181 cggtatgtga cgctcaacaa caggtacaag gcgtctttac cgacggcgat ttacgtcgct
    12241 ggctggtagg cggcggcgca ctcaccacgc cagttaatga agcgatgacg acaggcggca
    12301 ccacattaca ggcacaaagt cgcgccatcg acgccaaaga agttctgatg aaacgcaaaa
    12361 tcactgccgc accagtggta gatgaaaacg gcaaactcac cggcgcaatt aacctgcagg
    12421 atttctatca ggccgggatt atttaatcct tcaatcccag acgtttcgcc agccgatgca
    12481 ggttggcgac gtcagtttcc agcattcgcg cacaggctgc ccagttgtga tgattttgtg
    12541 ccagtgcctg gcgaatagtt tcacgctgga acgcttctgt cgcttcacgc aggttttgct
    12601 taacaacggg caccgccgcc gcttctggcg gcggcaacgt cacctcagga aaagcaaaat
    12661 gttgcgcctc aagaatcact tcatcgccgt tgcgggtggc tctcgccagc actaccgccc
    12721 gatgaatagc atgttccagt tcgcgcacgt ttcccggaaa actgtagtgt tgcagtaaat
    12781 ttcgcgcccc ggcacttaat accacgcggg agagcccctg ccgcaaacga cactgctcgc
    12841 agaaataccc cgccagcaga atgacatcat cgccccgctc acgcagcggc ggcaccgaaa
    12901 gtggaaacac gctcaggcga tgaaacaaat cggcgcggaa tcgccctgcc agcacctctt
    12961 cgcgtaagtc gcggttagtc gccgccagca cgcgcacatc gacccgcaaa ctacggtcat
    13021 tgccaacgcg ctgaatatcg ccatactgca acaccctcag cagcttggcc tgcaatgcca
    13081 acgacaactc gccgatctca tcgagaaaca gagtaccgtt atccgccatt tcaaacttcc
    13141 cgctgcggtt actgatagcg ccagtaaacg ctcctttcac atgcccgaac aactcacttt
    13201 ccgccacact ttccggcagt gcggcacagt tgagatagac cagcggattc accgcccgtg
    13261 gcgaggcttc atgaatcgct ttcgccacca gctccttacc ggttcccgtc tcaccgctga
    13321 tcagaacgtt gagatcggac gccgccacaa tctcaatctc ttttttcagt tgcgtcatgc
    13381 caggggacaa gccaatcatc tgcgtctgtt tcaccgcttc aaacggcgtg gcatcgcctg
    13441 gcagcatatt ctggctttcc agttgttcaa tcagcaacgc attgcttaac gctcccgccg
    13501 ccagcgcggc aatcagccgt aactcttcgt cgctgaaaac atcgaactga tcgggctgca
    13561 tcccgtcgag cgtcagtgcg ccgatcaggt tttgtccgac aaacaatggc agaccaacgc
    13621 aggcgtgaac cttcagactc tcctgcccag gaatcaaacc gtcataggga tcgggcaatt
    13681 cgctgtctgc gggaaagcgc accacatccc cggcgcgggc aatcgcttcc agccgtggat
    13741 gcccttccag cgcaaagcgt ctaccgagta catcctttgc cagaccgtcg atggcaagcg
    13801 gaataaactg ccgcgaatcg taacgtagca acgcagacgc atcgcactcc agcacctgac
    13861 gtagcgtggt gatcaggcgc tgaaaacgat cctgatgacc aatcccacgc tgcaattcga
    13921 tggcgatatt cgccagcaca tcaacggaaa aactcatctt tgcctcactg tcaatttgac
    13981 tatagatatt gtcatatcga ccatttgatt gatagtcatt ttgactactc attaatgagc
    14041 ataattttat ttatagagta aaaacaatca gataagaaac tggcacgcaa tctgcaatta
    14101 gcaagacatc tttttagaac acgctgaata aattgaggtt gctatgtcta ttgtggtgaa
    14161 aaataacatt cattgggtgg gtcaacgtga ctgggaagtg cgtgattttc acggcacgga
    14221 atataaaacg ctgcgcggca gcagctacaa cagctacctc atccgcgaag aaaaaaacgt
    14281 gctgatcgac accgtcgacc ataaattcag ccgcgaattt gtgcagaacc tgcgtaatga
    14341 aatcgatctg gcggatatcg attacatcgt gattaaccat gcagaagagg accacgccgg
    14401 ggcgctgacc gaactgatgg cgcaaattcc cgatacgccg atctactgta ctgccaacgc
    14461 tatcgactcg ataaatggtc atcaccatca tccggagtgg aattttaatg tggtgaaaac
    14521 tggcgacacg ctggatatcg gcaacggcaa acagctcatt tttgtcgaaa caccaatgct
    14581 gcactggccg gacagcatga tgacttacct gacaggcgac gcggtgctgt tcagtaacga
    14641 tgctttcggt cagcactact gcgacgagca tctgttcaac gatgaagtgg atcagacgga
    14701 gcttttcgag cagtgccagc gttactacgc caatatcctg acgccgttca gccgcctggt
    14761 aacgccgaaa attaccgaga tcctgggctt taacttacca gtcgatatga tagcgacctc
    14821 ccacggcgtg gtctggcgcg ataacccgac gcaaattgtc gagctgtacc tgaagtgggc
    14881 cgctgattat caggaagaca gaatcaccat tttctacgac accatgtcga ataacacccg
    14941 catgatggct gacgctatcg cccagggaat tgcggaaacc gacccacgcg tggcggtgaa
    15001 aattttcaac gtcgcccgaa gcgataaaaa cgaaatcctg accaatgtct tccgctcaaa
    15061 aggcgtgctg gtcggcactt cgacgatgaa taacgtgatg atgccgaaaa tcgccgggct
    15121 ggtggaggag atgaccggat tacgcttccg taacaaacgc gccagtgctt tcggatctca
    15181 cggctggagc ggcggtgcgg tggatcgcct ttccacgcgc ctgcaggatg cgggtttcga
    15241 aatgtcgctc agcctgaaag ccaaatggcg accagaccag gacgctctgg agttatgccg
    15301 tgaacacggt cgcgaaatcg cccgtcagtg ggcgctcgcg ccgctgccgc agagcacggt
    15361 gaatacggta gttaaagaag aaacctctgc cacgacgacg gctgacctcg gcccacgaat
    15421 gcagtgcagc gtctgccagt ggatttacga tccggcaaaa ggcgagccaa tgcaggacgt
    15481 tgcgccagga acgccgtgga gtgaagtccc ggataacttc ctctgcccgg aatgctccct
    15541 cggcaaagac gtctttgacg aactggcatc ggaggcaaaa tgagcaacgg cattgtgatc
    15601 atcggttcgg gcttcgccgc ccgccaactg gtgaaaaata ttcgcaaaca ggacgccact
    15661 attccattaa ccctgattgc cgccgacagc atagatgagt acaacaaacc cgacctcagc
    15721 catgttatca gtcaggggca acgtgccgac gaccttaccc gccagacggc gggtgaattt
    15781 gccgggcagt ttaatctgcg cctgtttccg cacacctggg tgacggatat cgatgccgaa
    15841 gcccatgtgg tgaaaagcca gaataatcag tggcaatacg acaagttagt gctggcaacc
    15901 ggtgccagcg cctttgtccc gcctgtgcct gggcgtgagt taatgctgac gttaaatagt
    15961 cagcaagagt atcgcgcctg tgaaacgcaa ctgcgggatg cccgacgcgt gttgattgtc
    16021 ggcggtggtt tgattggtag cgaactggcg atggattttt gtcgtgcagg caaagcggtc
    16081 acgctaatcg acaacgctgc cagtattctg gcgtcattaa tgccaccaga agtaagcagc
    16141 cgcttgcagc atcggttgac ggagatgggc gttcatctgc tgttgaaatc tcagttgcag
    16201 gggctggaaa aaacggattc tggcattctg gcaacgctgg accaccagcg tagtatcgaa
    16261 gtggatgcgg taattgctgc caccggactg cgcccggaaa cagccctggc acgacgcgcc
    16321 gggctgacga ttaatcgcgg cgtttgcgtc gatagttatc tgcaaaccag taatgccgat
    16381 atttacgcgc tgggcgattg cgcggaaatt aacggtcagg tattgccgtt cctgcagccg
    16441 attcaactta gcgggatggt gctggcaaaa aatcttctcg gcaataacac gccgctgaaa
    16501 ctaccggcga tgctggtgaa aatcaaaacg ccggaattac cgctgcatct ggcaggcgaa
    16561 acccagcgtc aggatttacg ctggcagatt aataccgaac gccagggaat ggtggcgcgc
    16621 ggcgttgacg atgctgacca gcttcgcgcc tttgtggtca gtgaggatcg gatgaaagag
    16681 gcatttgtat tgttgaaaac gttgccggtg taggtgggct actgtgccta aaatgtcgga
    16741 tgcgacgctg gcgcgtctta tccgacctac ggggacgcat gtgtaggccg gataaggcat
    16801 ttacgccgca tccggcaagg gtgtccaaat gcaacacgct ttatccgttc tggacttcac
    16861 ccgctaacca acgcgccgca gcaataaccc cctgccccag agacaaaccg ccatcacccg
    16921 ccggtaaact ctgtggaaag agcaatgtga aatcagcgag ataatgcgcc agacgtgcac
    16981 gcagcaaacg gttatgaata accccgccgc taaataccag cgtagtgata ccacgcatcg
    17041 tggcctgctc acgcatcaac gcggcaaaac cctgcgccag cgcatcatga aacgcccacg
    17101 cgcgttgatt aaccggtgcc tgccagttca gccactgctg ccagaaagtg gcgagatcca
    17161 gttgattgtc cacccgcggc atcgtcaccg gatgcgtcac tccgtcgcat gaggctgcga
    17221 gcgcctccag agcacaagcc gcttcacctt cataacttaa cgtggctggc gcacagccca
    17281 gtgccgccgc cactgcatcg aacaaccgcc cacacgatga tgccagcggc gcgttaattc
    17341 cacgctcaat ggcccgcgcc agtacgctcc agttttgctg ttgcacactt gccgtttcag
    17401 ggtaattctg ccactccggc acaaagcgca ggcactgcgc cagcaggttt cgccacggct
    17461 gcttcgccgc caaatcgcca cccggaagcg ccactgcagg caagccgccc aggtgctcac
    17521 attcgcgata gttcacccgc aggcactcgc cgccccacaa agcgccattc tcccccatac
    17581 cgataccgtc gagcgtcaaa gcaatgacat caccgccatc cagcggccac tgatgctctg
    17641 ccagacacgc cgctgcatgg gcatgatgat gcagtaccgt ttgcgtcggc agattcattt
    17701 cgctcgccca ctggctggag acatagcccg gatgcacatc atgcacaatg tattgcggag
    17761 tgaaatcgta gatgttttgc atcaggcgta acgcttcgcg ccactgcatc tggatgccat
    17821 cgtcacttaa atcgcccaga tgctgactca acaccacttg ttcaccgcgc accaggcaga
    17881 aggtattttt cagatccgcg ccaagacaca gcacaggcgg aacattttta aagcccggag
    17941 gcaaaaccag cgcatccggc acataccccc gcgaacggcg cagcatttcg ccgctttcgc
    18001 gcaccactga atcatccatc cgctgcacga tgtcgcggtt atgtagcaag aatccgtcgg
    18061 caatgccctg caaatccgcc agcgcctgtt cgttgctgat agctggtggt ttaccgctca
    18121 ggttgccgga ggtcatcacc agcgggcatt gcagttcctg taacagcaaa tgctggagcg
    18181 ggttcgcagg caacatcacc ccgacttcgt taaggccagg ggcgatatca tcacaaagct
    18241 caggtacgta ttttttatcc accagcacaa tcggcgcagc gggcgtggta agcaactggc
    18301 gcgcagcgtc tggtaaactt tctgccactg gcaacatgac tgccagcggt ttcgccgggc
    18361 gatgtttgcg ctcccgcagt gtcgccaccg cgttactgtt acgtgcatcg caggcaagat
    18421 gaaatccgcc aatccctttg atggcgacaa tgttgcccat ttttaactgt gcgatagctg
    18481 cctgtaatgc cgcctcttgt tctgcatgtt caccatgact tacccattca agatgcgggc
    18541 cacactccgg gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg tcgcggtact
    18601 ctttgtcaca ggccggacat agcggaaacg ccgccatcac ggtaaacggg cggtcgtaag
    18661 gcatcgcgcg aataatggtg aaacgcgggc cgcagtgggt acagttgata aacggataac
    18721 gataacgccg ctcgcctggg gtattcattt cggcaaggca agcagggcaa gtggcggcat
    18781 ccgggacaat ttgcgtattc atggtgccgc cggtgctctg gcggatggtg aactcggtgg
    18841 gcagttgtga ccagataaac ggctcacgct cgacgctatc aatacgcgcc agtggcggac
    18901 agtgctgatg caattgaaca agaaacgttt ccggatcttc cagcagccgg acttctacgc
    18961 catcgccgtc attacataca tcgccgtgaa gatttaattg ctgtgccagc tgccagacaa
    19021 acggacgaaa accgacgccc tgcactttgc cacgaatacg cagttggaca ccgcaaaatg
    19081 tgttttttgc cattgagtta ttcccgccat catgaattgc gtaacccgcc ctgccggaca
    19141 cgacagcgtc gtatccggca gtctcaggtc ggcgataccg cccgctccgt attctacgaa
    19201 tatttccggg aattcctttg atgccagaac agttctgtaa gatttttaga acatcagcgc
    19261 cgtacggcgg cgtttttctg cgctcagttg ttcaagttta ttacgatcga cacaaatcag
    19321 cgcatgggtc gggcaagccg ccatacacgc cgggccgtct tcacgatggt tgcacaggtc
    19381 gcatttattg gcttcggctt tgtcagcccg tacattcaga cccgcgccgc tgttgcgaat
    19441 cacaggacgt accaccactt ccatcgcgcc atacgggcaa gccaccacgc aggttttgca
    19501 accaatgcaa cgttcctgca tcacatgaac aaacccttta tcacagctga
//
LOCUS       DAJVQH010000075        19379 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJVQH010000075 DAJVQH010000000
VERSION     DAJVQH010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.075"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(33..1862)
                     /gene="glmS"
                     /locus_tag="OQM87_002615"
     CDS             complement(33..1862)
                     /gene="glmS"
                     /locus_tag="OQM87_002615"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCS1508701.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2024..3394)
                     /gene="glmU"
                     /locus_tag="OQM87_002616"
     CDS             complement(2024..3394)
                     /gene="glmU"
                     /locus_tag="OQM87_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCS1508702.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGAGMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3746..4165)
                     /gene="atpC"
                     /locus_tag="OQM87_002617"
     CDS             complement(3746..4165)
                     /gene="atpC"
                     /locus_tag="OQM87_002617"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCS1508703.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4186..5568)
                     /gene="atpD"
                     /locus_tag="OQM87_002618"
     CDS             complement(4186..5568)
                     /gene="atpD"
                     /locus_tag="OQM87_002618"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCS1508704.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5595..6458)
                     /gene="atpG"
                     /locus_tag="OQM87_002619"
     CDS             complement(5595..6458)
                     /gene="atpG"
                     /locus_tag="OQM87_002619"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCS1508705.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6509..8050)
                     /gene="atpA"
                     /locus_tag="OQM87_002620"
     CDS             complement(6509..8050)
                     /gene="atpA"
                     /locus_tag="OQM87_002620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCS1508706.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8063..8596)
                     /gene="atpH"
                     /locus_tag="OQM87_002621"
     CDS             complement(8063..8596)
                     /gene="atpH"
                     /locus_tag="OQM87_002621"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCS1508707.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8611..9081)
                     /gene="atpF"
                     /locus_tag="OQM87_002622"
     CDS             complement(8611..9081)
                     /gene="atpF"
                     /locus_tag="OQM87_002622"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCS1508708.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9143..9382)
                     /gene="atpE"
                     /locus_tag="OQM87_002623"
     CDS             complement(9143..9382)
                     /gene="atpE"
                     /locus_tag="OQM87_002623"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCS1508709.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9429..10244)
                     /gene="atpB"
                     /locus_tag="OQM87_002624"
     CDS             complement(9429..10244)
                     /gene="atpB"
                     /locus_tag="OQM87_002624"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCS1508710.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10253..10633)
                     /gene="atpI"
                     /locus_tag="OQM87_002625"
     CDS             complement(10253..10633)
                     /gene="atpI"
                     /locus_tag="OQM87_002625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCS1508711.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11250..11873)
                     /gene="rsmG"
                     /locus_tag="OQM87_002626"
     CDS             complement(11250..11873)
                     /gene="rsmG"
                     /locus_tag="OQM87_002626"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCS1508712.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11937..13826)
                     /gene="mnmG"
                     /locus_tag="OQM87_002627"
     CDS             complement(11937..13826)
                     /gene="mnmG"
                     /locus_tag="OQM87_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCS1508713.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14205..14648)
                     /gene="mioC"
                     /locus_tag="OQM87_002628"
     CDS             complement(14205..14648)
                     /gene="mioC"
                     /locus_tag="OQM87_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCS1508714.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14738..15196)
                     /gene="asnC"
                     /locus_tag="OQM87_002629"
     CDS             complement(14738..15196)
                     /gene="asnC"
                     /locus_tag="OQM87_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCS1508715.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15348..16340
                     /gene="asnA"
                     /locus_tag="OQM87_002630"
     CDS             15348..16340
                     /gene="asnA"
                     /locus_tag="OQM87_002630"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCS1508716.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(16345..17796)
                     /gene="viaA"
                     /locus_tag="OQM87_002631"
                     /pseudo
     CDS             complement(16345..17796)
                     /gene="viaA"
                     /locus_tag="OQM87_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(17790..19286)
                     /gene="ravA"
                     /locus_tag="OQM87_002632"
     CDS             complement(17790..19286)
                     /gene="ravA"
                     /locus_tag="OQM87_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCS1508717.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4630 a         5122 c         4861 g         4766 t
ORIGIN      
        1 ggtcggcagc ccttttttat ctatgatagt gattactcaa ccgtaaccga ttttgccagg
       61 ttacgcggct ggtcaacgtc ggtgcctttg atcagcgcga catggtaagc cagcagctgc
      121 agcggaacgg tgtagaagat cggtgcaatc acctcttcca catgcggcat ctcgatgatg
      181 tgcatgttat cgctacttac aaaacccgca tcctgatcgg cgaagacata caattgaccg
      241 ccacgcgcgc gaacttcttc aatgttggat ttcagttttt ccaacagttc gttgttcggt
      301 gcaacgacga taaccggcat atccgcgtca atcagcgcca gcgggccgtg tttcagctcg
      361 cctgcagcgt aggcttcagc gtgaatgtaa gagatctctt tcagcttcaa tgcgccttcc
      421 agcgcgattg ggtactgatc gccacggccc aggaacagcg cgtgatgttt gtcagagaaa
      481 tcttctgcca gagcttcaat gcgtttatcc tgagacagca tctgctcaat acggctcggc
      541 agcgcctgca gaccatgcac aatgtcatgt tcaatggagg catccagacc tttcaggcga
      601 gacagcttcg ccaccagcat caacagcaca gttaactgag tggtgaatgc tttagtggac
      661 gccacgccga tttccgtacc cgcgttggtc atcagcgcca gatcggattc gcgcaccaga
      721 gaagaacccg gaacgttaca gattgccagc gaaccaaggt aacccagctc tttcgacaga
      781 cgcaggcccg ccagggtatc cgcggtttcg ccagactgtg acaaggtgat catcaggctg
      841 ttacgacgca cggcagattt acgatagcgg aattcagagg cgatttcgac gtcgcacgga
      901 atacctgcca gcgattcaaa ccagtagcgg gaaaccatac cggagttata agaagtacca
      961 caggcgagga tctgaatatg ctcaaccttc gacagcagtt cgtcggcgtt cggtcccagc
     1021 tcgcttaaat caacctgacc gtggctgatg cgcccggtaa gggtgttttt tatcgcgttc
     1081 ggctgttcgt agatctcttt ctgcatgtag tgacggtaaa tacctttatc gcccgcgtca
     1141 tattgcagat tggattcgat atcctgacgt tttacttccg cgccagtttt atcgaagatg
     1201 tttaccgaac ggcgagtgat ttccgcaata tcgccctctt caaggaagat aaagcgacgg
     1261 gtcaccggca acagcgccag ctggtcagaa gcgataaagt tttcgcccat acccaggcca
     1321 atcaccagcg gactaccaga acgtgccgcc agcagggtat ccgggtgacg ggagtccatg
     1381 atcactgtac cgtacgcacc gcgcagctgc gggatagcac gcagaacagc ctcacgcaga
     1441 gtcccgcctt gtttcagctc ccagttcacc agatgggcaa tcacttcggt gtcggtttca
     1501 gaaacgaagg tatagccacg cgcttttaac tcttcacgca gcggttcatg gttttcgatg
     1561 atgccattat gcaccaccac aatgtgttca gaaacatgcg gatgcgcgtt agcttctgaa
     1621 ggttcaccgt gggtcgccca gcgagtgtga gcaataccgg tgccgccatg cagaggatgt
     1681 tcttccgctg cctgagccag catctggact ttaccgaggc gacgcaggcg agtcatatga
     1741 ccttccgcat caacaacggc cagaccggca gagtcatatc cgcggtattc cagacgacgt
     1801 aaaccttcaa gaaggatttc tgctacatca cgttgcgcga tcgcgccaac aattccacac
     1861 atagtttttg attccgattt atatcgttgt cggtcaacct gtatgcccgt tttttcgggc
     1921 gccccgagcc ttgtagagag tggggttatt tttatagtta ctgcttgtgg gagggagatt
     1981 atgttatctc ctcatcccag atgaccggat tacccggcca gaattacttt ttctttaccg
     2041 gacgacgcca gccttctttc tgagtctgcg gcacacggct gatagctaat gcattttcgc
     2101 cgacattacg cgtcacagtt gtacccgcag caatggtcgc gcctttgcct actgttaccg
     2161 gggccaccag ctgagtgtcg gaaccgacaa acacatcgtc gccgataatg gtcttaaatt
     2221 tattcgcacc atcgtagttg caggtaatgg ttcccgcgcc gatgttaacg ttatcgccaa
     2281 tttccgcatc gcctaggtaa gtcagatgcc cggctttcgt gcctttaccc agacgcgctt
     2341 ttttcatctc aacgaagtta ccaacgtgtg caccttccag caactcagca ccaggacgca
     2401 gacgggcaaa cgggccaata gtacaggccg ctgccagatt cgcgtcttcc acaacggtat
     2461 acggactgat ttcgcaatca tcgccaatca cgctgttttt aatcacgcaa ccggtgccga
     2521 ttttcacgcg atgaccgaga gtcacgttgc cctcgatgat aacgttagta tcaatttcaa
     2581 catcgcgccc gtgagtaagc gtaccgcgca gatcaaaacg cgccggatcg cgcagcataa
     2641 cgcctgctaa cagcagtttt tcagcctgtt cggactggta aacgcgctcc agacgggaga
     2701 gttgcaggcg gttattcaca ccttctactt cgcttaaacg ttgcggatga acggcgacga
     2761 tttcacgccc ttcctgatac gccagcgcaa taatgtcggt gatgtagtat tcgccctgag
     2821 cattattgtt ggtcagcttc gccagccagc gtttcatacc tgcgccgttg gcaatcagaa
     2881 tgccggtgtt gatctcctga atctgacgct gctcgtcggt ggcatctttg tgctcaacaa
     2941 tgccggtaac tttgccattt tcacgggtga tacgtccata accggtcgga tcatccagtt
     3001 tcaccgtcag cagaccaatg ccaccctggg gtttagcatc acgcaggcgc tggagtgttt
     3061 cgacagagat cagcggcacg tcgccgtaga gcattaaaat gtcttcatca tcggcaaaga
     3121 aaggtgcggc ctgctgcatt gcatgacccg tacccagctg ctctgcctga agcacccagt
     3181 taaggttgtc gtctttcagc gcctgtttaa gcagatcgcc gccgtgaccg tacaccaggt
     3241 gaacgtgcgc tgcgcctaat tcattcgcag catcaatgac atgctgaacc atcgctttcc
     3301 cggcaagggt atgcagcact ttcggaagat cggaatacat gcgcgtgcct ttgcctgcgg
     3361 caaggatcac tacgctcata gcattattca acatacgcgt cctgactgta atttgagaac
     3421 gaatttaaac cgcttcacct tgaaaaaact acattttttt catcgtgaaa tggacagagg
     3481 ataaattgtt caataacgga ttatccccgc tgacgaaagt gccattttcg accatcgtca
     3541 cgccttttgt ctgcagaaaa ttaaggcagt accttcattt aagcgtcagg tggatgtttt
     3601 tgctcttatt tcgatcaatg aataaacggg aaaacagggg ggttttatat cagcaggatc
     3661 tatgtgaacg ctcttcagga cgggtcaacg cgtaaaaaaa agccagcctg tttccagact
     3721 ggcttttgtg cttttcaagc cggtgttaca tcgctttttt ggtcaactcg ataacgcgca
     3781 gctgcgcgat cgctttggcc agttccgcag acgcctgagc gtaatctacg tcgccgtgag
     3841 agctgctaat gtgctcttca gccttacgtt tcgcttccat ggctcgcgct tcgtcgagat
     3901 cctggccgcg aattgcagtg tcggccagaa cggtcacgtt gccaggctgc acttcaagaa
     3961 tgccgccaga cagatagata aactcttcgt gaccgtgctg tttcacgatg cgaatcatac
     4021 caggcttaat ggcggtgagc agcggtgcgt ggccagggta gatccccagt tcaccttcgc
     4081 tacccgttac ctggattttc tcgaccagac cagagaacat ttgttgctct gcgctgacga
     4141 cgtccaggtg gtaagtcatt gccatatcac cctccgatta aggcgttaaa gttttttggc
     4201 tttttccaca gcttcttcga tggaaccgac catgtagaac gcctgctccg gcaggtgatc
     4261 gtattcgcct tccatgatgc ctttaaagcc acggatggtg tctttcaggg agacgtattt
     4321 acccggagaa ccggtgaata cttctgccac gaagaacggc tgggacagga agcgctggat
     4381 cttacgagca cgcgctacca ccagtttgtc ttcttcagac agttcatcca tacccaggat
     4441 ggcgatgatg tctttcagtt cctgataacg ttgcaggatg gactgaacgc cacgcgcggt
     4501 gtcgtagtgt tcctgaccaa ccaccaacgg gtccagctga cggctggtgg agtccagcgg
     4561 gtcaacggcc gggtagatac ccagagacgc gatctgacgg ctcagtacca cggttgcgtc
     4621 aaggtgcgca aaggtggttg ccggagacgg gtcagtcaag tcatccgcag gtacgtatac
     4681 cgcctgtacg gaggtgatag aaccagtttt ggtggaggtg atacgttcct gcagaacgcc
     4741 catctcttcc gccagggtcg gctgataacc taccgctgaa ggcatacggc ccagcagtgc
     4801 ggatacttcc gtaccggcca gggtgtaacg atagatgttg tcaacgaaca gcagaacgtc
     4861 acgaccttcg tcacggaatt tctcagccat ggtcagaccg gtcagagcaa cgcgcagacg
     4921 gtttcccggc ggctcgttca tctggccata caccagggat actttgtcga taacgttgga
     4981 gtcggtcatt tcgtggtaga agtcgttacc ctcacgagta cgttcaccta cgcccgcaaa
     5041 cacagagtaa ccggagtgct cgatcgcgat gttacgaatg agctccatca tgtttacggt
     5101 tttacctaca cccgcaccac cgaacagacc aactttaccg cccttagcga acggacacat
     5161 caggtcgata actttgatac cggtttccag cagttcctga gagtttgaca gctcttcgta
     5221 ggaaggtgct gcgcggtgaa tcgcccaacg ctcttcttca ccgatctcgc ctttcatgtc
     5281 gaccggttca cccagtacgt tcatgatacg gcccagagtc gctttaccta ccgggacttc
     5341 gatcgggtgt tcgaggtctt ttacatccag accgcgacgc agaccgtcgg aggaacccat
     5401 tgcgatggta cgcacgatac cgccgccgag ctgctgctga acttccagca ccagacgctc
     5461 attaccattt tgcacctcaa gagcatcgta cacgcgcggt acggcatcct gagggaattc
     5521 gacgtcaact acggcgccga ttacctggac aatctttcca gtagccatct taaatcctct
     5581 acgaaataac ctgtttaaac cgcggcggcc cccgagacga tctcggtgag ttcctgagta
     5641 atgctggcct gacgagcttt gttgtatacc aactgcagct ctttaatcag gctgccgcca
     5701 ttgtcggtcg cggctttcat cgccaccata cgggcggcct gctcgctggc caggttttca
     5761 accacgccct gataaacctg agattcgaca taacgacgca gcagggtatc cagcaacgcc
     5821 ttcggatcgg gttcgtacag gtaatcccag gatttatgtt tcagatcatc atcatctgat
     5881 gccggtaacg gcagcagctg gctgatggtc ggaacctgag acatggtgtt aataaatttg
     5941 ttgctgacaa tgtaaagctt gtccagacgg ccttcgtcgt aggcctgcaa catcactttt
     6001 accggaccga tcagttcgga cagggaaggg ttatccccca tgccggtgac ctgggcaaca
     6061 acattgccgc ccacggagtt gaagaacgac acgcctttcg agccgatcat tgcgaggtcg
     6121 cattgaacgc ctttgtcggt ccaggtcttc atttccgcca gcagtttttt gaacaggtta
     6181 atgttcaaac caccgcacaa accacggtcg gtcgacacca ccaggtagcc cacgcgttta
     6241 acgtcgcggt cttccaggta agggtgctta tattccagat taccgtgtgc aaggtgacca
     6301 atcactttgc gcatggtttc tgcataagga cggctggccg ccatgcgatc ctgcgattta
     6361 cgcattttgg aagcggcgac catctccatc gctttagtga tcttttgcgt gttctggacg
     6421 cttgcgatct tactacgtat ctcttttgcg ccggccatga gcttctcctc aatgccttgc
     6481 ggcctgccct aaggcaagcc gccagacgtt accaggattg ggttgctttg aaggaatcga
     6541 ggatgccttt cagcttgcct tcgatttcgt cgttgtagcc accggtctgg ttgatctctt
     6601 gcatcaacgg agcgtgatca cggtcgacgt aagccagcag agcggcttcg aagctgccaa
     6661 ttttcgacag ttcaacatcc gccaggtaac cacgttctgc tgcgaacaga accagagact
     6721 gctgcgcaac ggacatcggc gcatactgtt tctgtttcag cagttcggtc actttctgac
     6781 cgtggtcaag ctgcttacgt gttgcatcgt caaggtcgga tgcaaactga gagaacgctg
     6841 ccagttcacg atactgtgcc agagcggtac ggataccacc ggacagtttt ttcatgatct
     6901 tggtctgtgc tgcaccacca acacgggata cggaaatacc cgggttaacc gcaggacgaa
     6961 taccggcgtt gaacaggttg gtttccagga agatctgacc atcggtaatg gagattacgt
     7021 tggtcggaac gaacgcagaa acgtcacccg cctgagtttc gataatcggc agtgcggtca
     7081 gagaaccggt tttccctttc acttcacctt tggtgaaggc ttcaacgtat tcggcgttaa
     7141 cacgtgcagc acgctccagc agacgagagt ggaggtagaa aacgtcgccc gggaatgctt
     7201 cacgtcctgg cggacgacgg agcagcaggg agatctgacg gtaagcaaca gcctgtttag
     7261 acaggtcatc gtaaatgatc agcgcatctt caccgcggtc acggaagtat tcgcccattg
     7321 cgcaaccggc atacggtgcc aggtattgca gtgcagcgga ttcagacgcg gttgctacca
     7381 caacgatggt gttagccagt gcgccgtgct cttccagttt acgtaccacg ttagaaatgg
     7441 tggacgcttt ctggccgata gcgacataga tacatttgat accggaatcg cgctggttga
     7501 tgatggcatc gatagccagt gcggttttac ctgtctgacg gtcaccgatg atcaattcac
     7561 gctgaccacg accgattggg atcatggagt caacggcttt ataaccggtc tgtaccggct
     7621 gatctacgga ctgacgttcg ataacgcccg gagcgattgc ttctacagca gagaagccgt
     7681 cgtgatccag cggaccttta ccgtcgattg gtgcacccag agtgttaacc acacggccca
     7741 gcaggccacg gccaaccgga acttccagga tacggccagt acacttaact ttcatgcctt
     7801 cggcaaggtc agcgtacgga cccataacaa ccgcacctac agagtcgcgc tcaaggttca
     7861 gtgcgatagc gtaacggtta cccggcaggg agatcatttc accctgcata caatcggcca
     7921 ggccgtgaat gcggataaca ccgtcactta cagaaacaat agtaccttcg ttgtgagctt
     7981 cactcacaac attgaactga gcaatgcgct gcttgatcag ttcgctgatt tcggtggaat
     8041 tcagttgcat gctccagtcc ccttaagact gcaagacgtc tgcaaggcgc tcaagacgac
     8101 cgcgtacgct gccatcaatg accatatcac ccgctcggat gataacgcct gccattacag
     8161 acttatcgat tttgcaattc agcttaactt tgcgtgacag acgtttttcc atcgcagcag
     8221 aaattttcgc gagctgttgt tcactcagtg cggcagcgga aatgacgtct acctcagcgg
     8281 tagcctcact cacggcacga aggtgaataa actgctccag aacatccggg agcgcgttaa
     8341 gacgaccatt ttcagccatt acccgaatca ggttctgacc gttttcgtcc agttgctcac
     8401 cacaaactgc gataaacgac tcggcgagcg tttctggcgc aagcgcgcca gagagaagct
     8461 ctgccatttg ttcgtttttg gttacctcgg cggcaaacgc cagcatgtcc tgccagcgtt
     8521 ctacactttg gtgttcgacg gcaaagtcaa aagctgcttt ggcgtagggg cgagctaccg
     8581 taataaattc agacatcagc ccctccctcc ttacagttca gcgacaagtt tatccacgat
     8641 gtcgctgtta gcagcttcat ccacggaacg ttcgatgatc ttctcggcgc cagcaacagc
     8701 caggatagca acttgcttac gcagctcttc acgggcacgt ttacgctcgg cttcaatttc
     8761 agcctgcgcc tgggccacga ttttagtacg ttcctgttct gcctcagctt tcgcttcgtc
     8821 cagaatctgc gagcggagtt tattcgcctg ctcgatgatt acctgggctt ccgctttcgc
     8881 ttttttcagc tggtcggtcg cgctggcctt tgcaaggtca aggtccttat gtgctcgttc
     8941 tgcggaagca aggccgtcag caatttcttt ttgacgtttt tcgatggctg ccattaatgg
     9001 cggccatacg tacttcatgc agaacagaac gaacaggaca aacgcgatgg cctggccgag
     9061 gattgttgcg ttaagattca cagcacaatg cctctattta gttaacgttc tgatattgct
     9121 ctttaaataa aagcaacgct tactacgcga cagcgaacat cacgtacaga cccagaccta
     9181 cagcgatcat cgggatagca tccaccagac ccataacgat aaagaactga gtacgcagca
     9241 gaggaatcag atcaggttga cgcgctgcgc cttccaggaa tttacccccg aggatgccga
     9301 taccgatcgc agcaccgatt gccgccagac ccatcatcac agcggcagcc atgtacagca
     9361 gatccatatt caggttttcc atgacagtct ccagtttgtt tcagttaaaa cgtagtagtg
     9421 ttggtaaatt aatgttcttc agacgccatc gacagataga cgatcgtcag aaccatgaag
     9481 atgaaggctt gcagcgtaat gatcaggatg tggaaaatgg cccacggcac attcaggatc
     9541 cactgtgacc accacggcaa cagaccagca atcagaatga aaatcagctc accggcatac
     9601 atgttaccga acagtcgcaa accgagtgaa actggtttgg acagcaggct taccccttca
     9661 aggattaagt tgacaggaat gaacgcccag tgattgaacg gctgcagcgt caactctttc
     9721 gtgaagccgc cgatgccttt cattttgatg ttgtagaaca gaatcaggat aaatacgccc
     9781 agtgccatag acagcgttac gttcacgtcc gcagacggaa ccacacgcag tgcaggcaga
     9841 cccagtacat gttcagcaat gtacggcagc aagtcgatag gcagtaaatc catcagattc
     9901 atcaggaata cccagacgaa gatcgtcagg gccagcggag caatcagctt gcttttgcca
     9961 tggtacatgt ctttcacgct accattaaca aagccgatca ccagctcaat cgcggtctga
    10021 aacttacctg gcacaccgct ggtcgccttt ttggctacgc tacggaataa aaccaagaac
    10081 aacagaccca gcaccaccga gaagaacatg gagtcaatat tgattgtcca gaaggtggct
    10141 ggggggtttt gtggatccac cagcgagaat gtacgcaggt ccagctgaag gttattcagg
    10201 tggtgtccta tgtaatcctg cggcgtcata ttttctgaag ccatgatgcc ttttaccctt
    10261 tgttgttaat tacagccggt gccagtatct gaaccaccag caccaaaacc cacgtaacga
    10321 tcagcggcaa gaataccgcc tttaaaaccg ccaacgccac caccagtaac accaacatcg
    10381 ccagaacttt gaaagcttcg ccaaatgcga atgtccaagc cacccggcct ttcgctggtg
    10441 tatgcgcctg gtgacgccag gcaaatatca taaacaaaac gttaggcaga aagactgcca
    10501 ggcccccgct tattgcagag acgccccaga aggggtcttt gaggctgaac agcaatccac
    10561 ttgctatcac caccagtaac tgaacgagca gaagcttccg agcaacgttt cgactcacga
    10621 gcgacacaga catcacgttt ttcactcctg ctcccttcga ggtatgccgc gtgtcgtata
    10681 aaactttctt taaggcttag agtcaagcat caaaaagcgg tcaaattata cggtgcgccc
    10741 ccgtgatttc aaacaataag tagccaaaag gtgaataaat gtttaaatat ttttccagtg
    10801 catacaattg cgacttttcc gctacccctg ttcgatcatg aaaaactgta aataacgcgt
    10861 aaacactggt gataaagcgt gcttcagatc acatattgcg catgttcgcg cacagcatat
    10921 ttatttactt ggcaaatgat gcctttgcaa gtttatgata tttcagtcta aaaacagata
    10981 ctgttttaat aaatgacatt tacacaacaa aaaccaccca ttgacatttt taataatgtt
    11041 ttaacagcca atgatggttc ttagcgccga tttttagcag actgatattt tcactaatga
    11101 cttattttct gcttaccaaa aaaagccacg ttatcttgtt gatgcaaaag agtgaacgtg
    11161 gcgttaaatg taaccagtta tatcagtaga aaacctggtt gttgttaaca gtctaaccgg
    11221 tcaatttttt atgatttttt tgataaaaat taaattttat ttgctttaat caccaccaga
    11281 tgacgttcgc catccagggc tggaacctga agtttaacca ctgattcgac ctgatattct
    11341 tcgggcaaca aagcgatttc atcttccggc atttgccctt tcagcgcgta gaaacggcct
    11401 tgctcaccag gaagatggtg gcaccagctc accatatcgt tcagagaggc aaaagcgcgg
    11461 ctaattacgc catcaaatgg cggctctgaa ggaaactctt ctaccctgct ctgtactggt
    11521 tcaatattct ccagtttaag ctcatgttgc acctgacgaa ggaaacgcac gcgtttacca
    11581 aggctatcca acagagtgaa atgggcttca ggacgcacga tagagagtgg aatgcctggc
    11641 agtcctggtc cggtgccgac atcgataaac cgttcacctt gcagatacgg tgccaccaca
    11701 atgctatcga gaatatggcg taccagcatc tcattaggat cgcggaccga agtcaggttg
    11761 tacgctttgt tccatttatg cagcatattc acgtaggcaa taagctggtt tttctggtga
    11821 tcagtaagcg aaatacctgc gtctttcagc agtaaggaga gtttgttgag cacggtgatt
    11881 acctgttctt gatgcgttgc ttggtaagcg ggtgcttacc aggcattttt aatgcgttat
    11941 gcgctacgac gcagcatacc ctgttttttc agccacacca gcagaatgga gatggccgca
    12001 ggcgtgacgc cagaaatacg cgaagcctgg ccgatagagg ccggtttgtg atcgttaagt
    12061 ttggcgatca cttcgttaga aagaccggat acctggcggt aatccagtgt cgcgggtagc
    12121 agggtgttct cgttacgcag ctgcttttcg atctcatctt gctggcgcgc gatataacct
    12181 tcgtatttaa cctgaatctc aacctgttcc gccgcctgtt cgtctgtcaa cgcaggggca
    12241 aacggcgtca gcgtggttaa tttttcataa gtcatttccg gacgacgcag cagatcttca
    12301 ccactggctt cacgggaaag cggcgcagtc aggtgagcat tcacttcggc tgcagcttcc
    12361 gccgacgggg ttacccaggt cgatttcaga cgctgacgct cacgctcgat attctcaagt
    12421 ttctcgttaa agcgcgccca acgttcgtca tccaccaggc ccagttcacg accgatttca
    12481 gtcaaacgca gatccgcatt atcttcgcgc agcatcagac gatattctgc gcgcgaagta
    12541 aacatacgat acggttcttt ggttcctaaa gtgcacaggt catcaaccag tacgccgaga
    12601 tacgcctgag aacgtgccgg agcccaacct tctttgtcag cagacagacg ggcagcgtta
    12661 agaccggcca gcaaaccttg cgcagcggct tcttcgtaac cggtagtgcc gttaatctga
    12721 ccagcaaaga acagcccctg gataaacttg ctctccagcg tcggtttcag gtcgcgagga
    12781 tcgaagaagt catactcaat ggcataaccc ggacgcacga tcttcgcgtt ttccatcccc
    12841 tgcatagagc ggacgatttg catctgcaca tcgaacggca ggctggtgga gataccgttc
    12901 ggataaattt cattagaggt cagtccttcc ggttcaagga agatctgatg ctgatttctg
    12961 tctgcgaagc gcatgacttt gtcttcgatc gacgggcagt agcgtgggcc gacaccttcg
    13021 atcacccctg catacattgg gctacgatcg aggttactgc ggatcacatc atgggttttc
    13081 tcgttggtat gagtgatata acacggcacc tgctggggat gcagggacgc attgcccata
    13141 aacgagaata ccggcattgg gttatcgcca tgttgttgcg ccagcacgct aaagtcgatg
    13201 gttcgagcat caatacgcgg cggagtaccg gttttcagac gaccaacgcg cagcggcagt
    13261 tcacgcaaac ggcgagagag cggaatggat ggcggatcgc cagcacggcc accgctgtaa
    13321 ttatccagac cgatatgaat tttgccgtcg aggaacgtcc caacggtgag caccacagct
    13381 ttggcacgga acttcagtcc catttgggta acagcaccga ccacgcgatc gttttcgaca
    13441 ataagatctt caaccgcctg ctggaagatc atcaggttcg gttggttctc cagcgccgta
    13501 cgtaccgcct gacggtagcg cacacgatcc gcctgagctc gggtagcgcg aaccgccggt
    13561 cctttgcttg cgtttagtat cctaaactgg atacccgctt gatcgatcgc tttcgccatc
    13621 agaccgccga gtgcatccac ttcttttacc agatgtccct tcccaatacc gccgatcgcc
    13681 gggttgcagc tcatctgccc cagagtgtcg atattgtgtg tcaaaagcag agtctgttga
    13741 cccatacgcg ccgcagccat cgcggcctcg gtgcctgcat gacccccgcc aatgatgatg
    13801 acgtcaaaag gatccggata aaacatggtg attgcctcgc ataacgcggt atgaaaatgg
    13861 attgaagccc gggccgtgga ttctactcaa ctttgtcggc ttgagaaaga cctgggatcc
    13921 tgggtattaa aaagaagatc tatttattta gagatctgtt ctattgtgat ctcttattag
    13981 gatcgcactg ccctgtggat aacaaggatc cgacttttaa gatcaacaac ctggaaagga
    14041 tcattaactg tgaatgatcg gtgatcctgg accgtataag ctgggatcag aatgaggggt
    14101 tatacacaac tcaaaaactg aacaacggtt gttctttgga taactaccgg ttgatccaag
    14161 cttcctgaca gagttatcca cagtagatcg cacgatctgt atacttattt gagtaaatta
    14221 atccacgatc ccagccattc ttctgccgga tcttccggaa tgtcgtgatc aagaatgttg
    14281 atcttcagtg tttcgcctgt ctgttttgca ccggaatttt tgagttctgc ctcgagttta
    14341 tcgatagccc cacaaaaggt gtcatattca cgactgccaa taccgattgc gccaaagcgg
    14401 accgcagaaa gatcgggctt ctgttcctgc aatgcttcat agaaaggaga aaggttgtcc
    14461 ggaatatctc cggcaccgtg ggtggagctg ataaccagcc agatccccga ggcagataaa
    14521 tcttccaaca gcggaccgtg cagcgtttcg gtggtaaaac ccgcctcttc cagcttttca
    14581 gccaggtgtt ctgctacata ttcggcaccg ccgagggtgc tgccgctgat aagagtgata
    14641 tctgccataa accgccacct ttattaagag tggcgtattg tacgctgtga acgcgttggg
    14701 atctacctgt ggaaaagtat gggattaaaa aagccgatca gggcttgatg gtacgcatga
    14761 tcgggttttg caggacgatc aatgtctcgg tggactgaat ttcatcaatt gtttggatct
    14821 tgttgataag tacatgctgg agagcgtcga tcgaacggca cataactttt ataaagatgc
    14881 tgtagtggcc ggttgtgtaa taggcttcag tgacttcatc aaggctttcc agctttgcca
    14941 gcgcggaagg gtagtctttg gcgctcttta atataatgcc gataaagcag cctacgtcat
    15001 aaccgagctg cttcgggctg acatcaatac gcgccccggt aatgatcccc gcctgcttca
    15061 ttttctctac tcgaacgtga atcgtccccg gactgacgcc aaattgtttc gccagttcgg
    15121 cgtaagcggt gcgcgcattg cccattaatg cttccaggat gccacggtcc agattgtcga
    15181 tcagataatt ttccatagga ttttcttatg cagattgatg attcattcta ttttagcctt
    15241 cttttttaat gaatcaaaag tgtgttaggc tttttattga atgattattg catgtgtgtc
    15301 ggtttttgtt gcttaatcat aagcaacagg acgcaggagt ataaaaaatg aaaaccgctt
    15361 acattgccaa acaacgccaa attagcttcg tgaaatctca cttttctcgc caactggaag
    15421 aacgtctggg gctgatcgaa gtccaggcgc cgattcttag ccgtgtgggg gatggcacgc
    15481 aggataactt gtcgggctgt gaaaaagcgg tgcaggtaaa agtgaaagct ctgcctgatg
    15541 cccagttcga agtggttcat tcactggcga agtggaaacg tcagacctta gggcaacacg
    15601 acttcagcgc gggcgaaggg ctgtacacgc acatgaaagc ccttcgcccc gatgaagacc
    15661 gtctttctcc gttgcactcg gtctatgttg accagtggga ctgggaacgc gtaatgggcg
    15721 acggtgagcg tcaattctcg actctgaaaa gcacggtaga ggcgatctgg gcgggaatta
    15781 aagcaaccga agctgcggtt aacgaagagt ttggcctggc accgttcctg ccggatcaga
    15841 ttcacttcgt acacagccag gagttactgt ctcgttatcc ggatctcgat gccaaagggc
    15901 gtgagcgggc gattgcgaaa gatcttggcg cggtattcct cgtcgggatt ggcggcaagt
    15961 tgagtgatgg tcatcgtcac gacgtgcgcg caccggatta tgatgactgg agcaccccgt
    16021 cagagctggg ccatgcgggc ctgaacggcg atattctggt gtggaacccg gtactggaag
    16081 atgcgtttga gctttcttcc atggggatcc gcgtggatgc cgacacgctg aagcatcagc
    16141 tggcgctgac cggtgatgaa gatcgtctgc agctggaatg gcatcaggcg ctgctgcgcg
    16201 gtgaaatgcc gcagaccatc ggcggcggta tcggccagtc tcgtttgact atgctgctgc
    16261 tgcaactgcc gcatatcggc caggttcagt gtggagtatg gccagttgct gttcgcgaga
    16321 gcgtcccttc tctgctgtaa taatttatcg ccgccagcgt ctgagcaggc ggcttcgcat
    16381 cccggtatca aagcgccaga tatgatcgaa aatgcgcatg atgccgggtt tgccgtgtgc
    16441 cgacatcgcc acggcatgaa agcgatgctg atgtacccgc tgcagctctt tcactttact
    16501 cgtcacgtcg tcaggcaacc gctgagcgat aaaatcagaa atcaccaccg catcggcatc
    16561 aaaccattcc ctgctttgca agcgttccat aatggcgcga aaacaactgg caagatcggt
    16621 gccgccacga aactgctggc ttaaaaaacg gattgcttgt tcgatgcctt gtgggcctga
    16681 aagctcataa cggacgatct cggtggaaaa tagcataata taacagcgcc ggttttcagc
    16741 gagagcaatg cgcatcaaag ccaggcagaa cgctttcgca cactgttcat taaagccgcc
    16801 cattgagccg gaagtatcca cacagacaat aaacggcccg cgcggctgtt catcgtaatc
    16861 tttatgtacc accggacgtt cgagcacttt ttcacgccac gactcaccgt gcaggcgata
    16921 ggtgagcaac tgtttttcca ccagccgacg gtaaaactca tactccagtt ccgttatccc
    16981 tagtgtcgcc agttctggcg gcaggagacg taaaatatca tcgctttgtt gcagaccatc
    17041 aacctgctca ggaaccgtcg ccggttcgcg caccatggtg cggaaggttt ccatctacgc
    17101 atcgttgcgc ggtattgatt tggcttcccg agaacgcccc ggctgctctg ccaggcgttt
    17161 cagttccggc tgttcgttaa gaaattcacc gtatttcaca ataaactgat agtcgccacg
    17221 tttaagctga ccggcgctca tatcccacag acgaccagct gcggtattgt tatctgcgag
    17281 aatcggttca agttgtccgc tcagcgtcat gcgttcctga acttcactta acagttgttc
    17341 gcgttcttct tctaatagct gttgatttaa cgtcgttgct tgcacgatca gacttaaacg
    17401 ccaacgctgg agaaaaagcg tgtgtaacgc tgaagttatt gtgctgttag catcaaccaa
    17461 ctgccgggct tgttctgccc atggggaatt cagacgatgc agtaagtcta ggatctgtgg
    17521 tagctgcaca ataaactgcg gcgtggagag gagctggctt tgctgatagc acatcacctc
    17581 ttcggtgagt tccggcggga ctcgggcatc tttcagccga ctgcgcagcg cctcacgcca
    17641 gcggggaaca tcatcagtga ttgccgcctt cagtcgtggg aatttttcaa agaagaccgc
    17701 cagctgcggt gaggccagca gtgcgatgat catctcttcg atcaatccct cttcgctgac
    17761 ggccagcatc acattaagcg tatccagcgt tagcattgtt gtgcctggcg aatctgtgca
    17821 ccgacatcct gtaggctggc ttcaatgcga cctaaccagt cgccagggat aaacagacat
    17881 ttttgctgtt cgctgaataa agcgtgttgc ttacgccagt cgctttcaag ctcttccagt
    17941 tgttgtttta tttcacctgg cagaccttca gccgatgaac cgggaagtgc cagcgtactg
    18001 ccttgtaaac ttacatcgcg tacaacaaga tgttgggtgc tatcaacttc cagattcagt
    18061 ttctgggcaa agccgatacc gttcagtttg ccgcgaattt caccaccttt actcagccac
    18121 tgttccagcg cgctacgctc aaaggagata tgaaccactt ccatatcatg cagttttaac
    18181 ggtttttgca gcagcagagt cagagtggaa gcagtaacgt taacaggaag ttgatactgc
    18241 tgacgacggc tgaaaatgcc gcccagacga attaacgtta aggctgtttt atcgctttgt
    18301 tgctgctgga gttgcaggtg acgttgcaca atcgcgccca ggcgggtcaa catcccttgc
    18361 tgttgccagg catgaccggt catcaataca tcaatttgtt gttgtatcaa attcaggctt
    18421 tgcgcgtcat accacaggca atctttcagc aaaatgagat caaccggggc aacagcactg
    18481 cgaccgctaa aaaaggcgct ggcctgcaat aagcggatcg cttttttcca gcgacgatcc
    18541 gagacataag gcgcatccgg taatttatcc agttgctggc gcagcataaa aatcagctca
    18601 aatacatgat cgggcagcgt aatttcacca atctctttct gccagcgttc atattcttca
    18661 tctgtgacct gcaaggcatc aggaaccgga ttgtcgtttt catcctgttg actggtcagc
    18721 atggagcgga aattcgcttt atcttgcact ttatctaacc atagacgaat cagcatgcgg
    18781 tcatataacg cttccagact gctgtctgct tccggcagct cgttggaggc cgccaccagt
    18841 agacgcatcg ggattttttc tacgtgtgca ccgttgcgga actggcgctc gttaatggcg
    18901 gtgagcaagg tattaagaat tgccgggccc gctttccaga tctcatccag aaagacgatt
    18961 tctgcttccg gcaggtaacc gctggttaaa cgttcatagc gcccttcatc ttttagcgcc
    19021 tgaatagaaa ggggaccaaa aacttcttcc ggcgtggaga agcgggtcat cagatattca
    19081 aacgcgcggg cattctgaaa ggcgaatttt aagcgccggg cgatcaaact tttggcaata
    19141 cctggcgggc caaggaggaa cacactttca ccacttaatg ccgctaataa acacaagcgg
    19201 atggcgtggc tacgttcata aagccccttt tccaacgaac tgctcaggcg ggaaattctt
    19261 tccgctaata aatgagggtg agccataatg aagtggcgtc ctttcgtcaa aagttctgcg
    19321 taaattgcga gtatagacgt ttcttgctgg tggctaaaat agtctcaaag gggggtaat
//
LOCUS       DAJVQH010000076        17831 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJVQH010000076 DAJVQH010000000
VERSION     DAJVQH010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.076"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..550)
                     /gene="aroD"
                     /locus_tag="OQM87_002633"
     CDS             complement(<1..550)
                     /gene="aroD"
                     /locus_tag="OQM87_002633"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCS1508718.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLT"
     gene            complement(581..1447)
                     /gene="ydiB"
                     /locus_tag="OQM87_002634"
     CDS             complement(581..1447)
                     /gene="ydiB"
                     /locus_tag="OQM87_002634"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCS1508719.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1459..2730)
                     /locus_tag="OQM87_002635"
     CDS             complement(1459..2730)
                     /locus_tag="OQM87_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1508720.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(2913..4117)
                     /locus_tag="OQM87_002636"
                     /pseudo
     CDS             complement(2913..4117)
                     /locus_tag="OQM87_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4217..4573)
                     /locus_tag="OQM87_002637"
                     /pseudo
     CDS             complement(4217..4573)
                     /locus_tag="OQM87_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4709..4816)
                     /gene="rprA"
                     /locus_tag="OQM87_002638"
     ncRNA           complement(4709..4816)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OQM87_002638"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5002..6114)
                     /gene="ydiK"
                     /locus_tag="OQM87_002639"
     CDS             complement(5002..6114)
                     /gene="ydiK"
                     /locus_tag="OQM87_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCS1508721.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAILAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6503..9559
                     /locus_tag="OQM87_002640"
     CDS             6503..9559
                     /locus_tag="OQM87_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1508722.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVVLIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9556..9966
                     /gene="menI"
                     /locus_tag="OQM87_002641"
     CDS             9556..9966
                     /gene="menI"
                     /locus_tag="OQM87_002641"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCS1508723.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10066..10254
                     /locus_tag="OQM87_002642"
     CDS             10066..10254
                     /locus_tag="OQM87_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCS1508724.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            10802..11170
                     /gene="sufA"
                     /locus_tag="OQM87_002643"
     CDS             10802..11170
                     /gene="sufA"
                     /locus_tag="OQM87_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCS1508725.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11179..12666
                     /gene="sufB"
                     /locus_tag="OQM87_002644"
     CDS             11179..12666
                     /gene="sufB"
                     /locus_tag="OQM87_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCS1508726.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12676..13422
                     /gene="sufC"
                     /locus_tag="OQM87_002645"
     CDS             12676..13422
                     /gene="sufC"
                     /locus_tag="OQM87_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCS1508727.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13397..14668
                     /gene="sufD"
                     /locus_tag="OQM87_002646"
     CDS             13397..14668
                     /gene="sufD"
                     /locus_tag="OQM87_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCS1508728.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14665..15885
                     /gene="sufS"
                     /locus_tag="OQM87_002647"
     CDS             14665..15885
                     /gene="sufS"
                     /locus_tag="OQM87_002647"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCS1508729.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQAQGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            15898..16314
                     /gene="sufE"
                     /locus_tag="OQM87_002648"
     CDS             15898..16314
                     /gene="sufE"
                     /locus_tag="OQM87_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCS1508730.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16463..17467
                     /gene="ldtE"
                     /locus_tag="OQM87_002649"
     CDS             16463..17467
                     /gene="ldtE"
                     /locus_tag="OQM87_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCS1508731.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17531..17767)
                     /gene="lpp"
                     /locus_tag="OQM87_002650"
     CDS             complement(17531..17767)
                     /gene="lpp"
                     /locus_tag="OQM87_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCS1508732.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4685 a         4320 c         4592 g         4234 t
ORIGIN      
        1 acgtcagcac atcgctggta ctttgcggca tcagcgcaat cttaggaata tcggcgtcga
       61 aggactgcat tttgcgcaga cgggcaatga tttcttcggc ttccggcgtt ttatggaagt
      121 catggttgga catgacaact ttcacatcat gcgcgtgggc gtaggcgacg gtttctttga
      181 cctgatcatc gccggtaaat aactccagat cgatcatatc aaccaggccg ctgtcgatgg
      241 ctgcacgatt gagagcaata taagcctcgg tggaaatcgc ctgctcgccg ccttctttgg
      301 cactgcggaa ggtaaacagc agcggttttt ctggcatggt ttcacggaga atttttgccg
      361 ccgccatgac agactccaca ttggagaggt cggcaaagtg gtccacacgc cattccagaa
      421 tatcaaagtc cgcttcacga taggcgagag cttcggattt cacgcgggcg atatctttcg
      481 ccatcagcga gacgatgatt ttaggtgcgc ccgcaccaat gacgagatct tttacagtta
      541 cggttttcat tttttaccct ttctgcacgc ggtcagcctg tcaggcaccg aaccccatga
      601 cctgtttaac atattccaga gggaaatctt tgccagtcca taacgtgaac tgttcagccc
      661 cttgccacaa caacatgccg tatccatcaa tcgttttgca accagcttgt tgtgcctgct
      721 gcaataactt cgtcatatgc gggttataca cgcattcagt gaccagcagt cccggatgta
      781 acagactgat atcattaacc aatgattcat tctcaagggg tttcatacct acttttgtgc
      841 cattggttaa aatgtcggcg gaagccaggg cttcagcaaa ggcttgctga tcggcgagat
      901 cggtaaccgt gacgacacaa tcggtgtttt cgttaacccg ctgcgcgaag gcgagggctt
      961 tatcgaagaa ctcatcccga cggttaaaga gtttaatttc ttttaaacct tcaattgccc
     1021 cctgcgcgcc aatcgccgtt gaggcacctc cggcccctaa cagcaccatc gttttgcctt
     1081 tgatatcaaa accgctctct ttaatggcgc gaatatggcc cgtgccgtcg gtgttatagc
     1141 cacgcagata gccatcatca ttaacgatgg tgttgatggc acccaccagt ttggctgctg
     1201 gcgttaattc atcaacatat tcacacgcca gttgtttgtt cggcatcgac acaccagttc
     1261 cgcgcatttt gagggctttt aatccttcaa ttgctccggg aaagctatcg ttatccactt
     1321 cgaaggccat ataggtaaat ggcaatcccg ctttttctaa ggctttattc tgcatttcag
     1381 gcgataaact gtggcggata ggataagcca tcaatccaat caattcgtat tttgcggtaa
     1441 catccataat taactccttt aacctctatg cttaattgtg tttaaccggg tgctaaattt
     1501 gcgttcgcca aaccgcacat cattctcagg aataataaaa acgcggtaat agcggctaaa
     1561 aacaataatt gcggtaatca gggccagcag cgcgaaagta aaatcgagaa caatgatata
     1621 ttgcaggccg atgttcgaca gataaccggt aatcagcgga ataacaaagt tagccagtcc
     1681 acccatcatc atataaatac tggtgacttt ggctttgctt ttggggaaaa actctgacat
     1741 gaccgaaaca ccgagctgta aaatgccgcc agctgctgag aaaccgataa caaaggcgcc
     1801 ggcattacac accagtggag aagggtacag gtagataatg gcagctgtta ttgttgccag
     1861 cgcagagtta aatacattag cccagatggg ccggaccatt tttttcagta gtgcggcaaa
     1921 aataaagaca cagaccaacg agcccatact gtaataagag atggttttta atgcctcagc
     1981 ttctgacata ccagcaaaag ccatcgcata tttgggcatc cacaccacaa tcacataaaa
     2041 ggtcgagaat gcggctacac cgaacagtac cgatgaaaca ccttccagcc agactaacgg
     2101 tttgctgttc atttgcggta attcattagc tacgctggcg tccaccaact ggctggggaa
     2161 tttgcttttc aacagcatca gcgtgatcag tacaaacaga atacccggaa taatcagccc
     2221 atagccgtac cagatattat tgagcaacat atagctcacc agcattgggt agaacatttg
     2281 cccaaatgac accatcgctt taaccagtat gaccgccgaa ccagaggctt tcggaaagca
     2341 ttccatgagc gcggggtagc cacccgtatc cagcgctgag ttagcgatac ctacgcacac
     2401 tgccagaccg taggcgagag ttaaattcgg gcaagcggga ataccaaaga agaatagcag
     2461 atacattatt actgccatta atatcaccgc ccgacgacca aacttatcgg agatcacacc
     2521 gaagaataaa atactgatca atcgccccaa accgataccg gaaattaaat aggcaatgcc
     2581 cgcgttgtca gtggaaaact tttctgccag agatgtcata ttttgggcaa gcgtaataac
     2641 actaatgccg tgcaggaaat agctgaagta aatacaaaga acagccagga taaatggcgt
     2701 gctgaaagat aatttcgtta ccgtacgtat tgttctccga cggtttacat aattactata
     2761 ctgaagatga ttttccatgg gtacatggca taaagtttat gtttccagga atattgcatg
     2821 tcatagtttg tttttgatcg cgattttcct tagttagcgc agaaagagtt atttatccgt
     2881 aagatcttgc atgacctgaa aagaatattg cattacccac ccggagcgac attttccttt
     2941 agcgagtgat gccgcgtttt tttgcggctg cgtaagccga taaacagtgc cagtaaaaaa
     3001 ccgatggcag tatcgaacca cataatatcg gcaatacttc tttgggacag atgagccgta
     3061 atcaacggaa tagtaaaggt cgcaatactg cccgcactgt aatagatccc tgtagcttta
     3121 cctttagcgt aagggaaacg ttcagctatt aacgtcaggc caatttgcac aacacctcca
     3181 gcagaggtaa aaccaatgac aaaagcaaat attatcacca cataaaatgt gggatgcagg
     3241 cagacggtaa acagagcaat aaatgagata aaggtgtaca gcatcagtaa tgttgttggg
     3301 cgaacggtat tacgaatgag tggagcggta ataaatacac aaagcagcga acccacggtg
     3361 tagatactga gtagtttgat cgacatagtg tatgacatgc ctgcaacaaa ttgtccgtac
     3421 tgtgccagcc actggctaac cagataaaac gttgccattg agatatagcc atataaggta
     3481 taactggcta aatcgataat tgaacagcga tgttccgtag agctggtggt tttctttatg
     3541 acaggtaagc gacgacccgg atggggtggg aacgtacaac ggtataaaaa cagagcgtta
     3601 ataaacataa tgcctgcagc aatcataaag gaccaaccga accacagttc agcccacact
     3661 aacaggctaa tgattagcgg taataaaaat tgtccgctgg aaacaaatgc tttaattaaa
     3721 atattggctg tcccaggtga gcgtggaaaa gcttccatca aactgggata agtgcctgca
     3781 tcgagaaaac tgtttgccat tcccgccaga aagccaaaaa cataagcgat aatgatgtta
     3841 ttggtctgca ggatgccaaa aaagaaggcc atatagcagc acattccgag catgataaaa
     3901 gggcggcgac caaagcgatc ggataataat cctgcaaaaa gcaagacact taatcgacca
     3961 atgcccagcg atgagataac tatcgagaca cccgcggcat tagtctgaca aagtgtctcc
     4021 agcgaggcca tattcaggct catcaaaatg acgcccatac catgcaccag gtaataaaat
     4081 acaacccaag tgcggtaggg taatagggat ttttcatagg ttctacttag caaaggtgct
     4141 ttaaaaagtg tagcgatgtt gtgtcagtat ccattcatta ttgttgtaac aacgacaatt
     4201 cctttctgtg gaaaaattaa caaagccgct ccagatcgtg tgcatataat tcagcggcta
     4261 ccgattgata gatcttccag tcaataaaat tgccatcagg gtagacttgt tgaaacgcgg
     4321 ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg gttatttatt ttctctatga
     4381 tggcatgaag atgttttttt cgttactgac gcatagccaa cagttgctcg actacacaat
     4441 caggaatggc gcatttgcca ttttcccact gacgccagct ttcactgtca ccggtctggg
     4501 caatccaggt cgcgcattca tcaatagtca tggcaaaaat atggcggagt gcctggagtt
     4561 cataagcgtt catcgtgtaa tggctgcatg aaataaacaa taaaatataa ataacattat
     4621 cagcctgctg acggcttgaa gagagtcaca gtatcttgtg caacattatt ggaaagagtg
     4681 aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc ccatcgtggg agatgggcaa
     4741 agactacaca cagcaattcg ttgtttcact caggggattt ccatgcttat aaatcaatat
     4801 gttgatttat aaccgtgagc taatagtagg catacggact ttttgcgtcg atcagattcg
     4861 tctcattagt ttaaaaactg taaagaattt gcgacaggta ctaccagctg aatggcggta
     4921 agcaggaaaa tatggggaag agcaagcaaa tatcaattag cggtgattaa caatcaggcg
     4981 gcagagccgc ctgatgagaa attacttatt cggtttctcg atttcgccaa gttcttcgag
     5041 aatttcttcc ggttggtccg tcggcggcgg gacttcttcc acccacgcgg caaacagacg
     5101 ccaggaaacg gctaacagaa ccggaccaat aaacagaccg atcatcccga aagcaatcaa
     5161 accaccaata acgccagaga ggatcaggat cagcggtaaa tcggcaccca tgcgaattaa
     5221 cattgggcgg atgacgttat ccagcgtgcc aaccacaccg ctccacacta acaataccgt
     5281 tccccaggtg gtatcgccgg tccagtagag ccagataatc gccggaatca gtaccggcaa
     5341 cgggccaagc tggacaaggc aggagaggat cattaacacc gttagcagcg ttgcataagg
     5401 tacgccggat acggcgaggc cgataccgcc aagcactgcc tgtactaacg ccgtcaccac
     5461 cacacccagc gccaccgcga ggatagcctg tgccgccagc agcacggcgg catctccgcg
     5521 aacgcctgcc agacgggttg caaaatggcg aatgccttgt gccacctgtt caccgcgcca
     5581 gtacagcagg gcactgaaga gaagcatcag cgcacaatgc accataaagc gcccgatatg
     5641 cgccgcctgc ccaacgaacc aagtggtggt ggtgccaata taagggcgga ctttcgccat
     5701 gatcgccgta ccccccatat ccagcaagtt gtgccagcct gcatacagct tcgcgccaat
     5761 caccggaatg gtattaagcc acgccagatc gggtaacgtc atgtcgccgc tggaaatggc
     5821 tttaattagc gggccgctgc cgtcgacgat actgttaacc agcaaagcaa tagggatgat
     5881 aaacaccatc actaataaca gcgtcatcac cagaacggcg agagagcggc ggccaaacat
     5941 gatcttttgc aaacgtaaca ataccggcca ggtggcgata accaccgtac cggcccatgc
     6001 aaagccgaga ataaagggtt gaacaatcca cagacatgcc acaatcatga tggctaaaaa
     6061 cagcaccgaa agcagaattt gtgcgacatc cctgggctga cgaacattta ccataactac
     6121 ttttcacctt ttttgtgcgt caaatcgtcg acgcgacgtg gacacgcgaa actcacctgc
     6181 ataatgatga gcaatttcag cggtttttaa caggccgatt ctgtccttaa ttctgatggg
     6241 cgtgttagaa aaaaatgtgg tacaacaact caggcaacac gcaaacgatt tactcgtcgt
     6301 atttcaagtt gcatgtgctg cggctacgtt cgttcacccc agtcacttac tattgtaagc
     6361 tcctggagtt cactcgcttg ccgccttcct gcaactcgaa atccatagag taaataaaca
     6421 tcacctttca gtgcgctatt gcagccgcct gaaagaatag tgtatcgacc tacatcacag
     6481 accgcaggaa agggtcaata taatgattcc acagatttcc caggcacccg gcgtcgttca
     6541 actggtgctt aattttttgc aagagctgga gcaacaaggt tttaccggcg atgcggcgac
     6601 aagttatgcc gatcgtctga caatgtcgac cgacaacagt atttaccaac ttctccccga
     6661 tgcggtggta tttccgcgtt caaccgcaga tgtggtgctg atcgcccgtc ttgccgcgca
     6721 ggaacgctat tcatcgctga tctttacccc ccgcggcggc ggcaccggca ctaacggtca
     6781 ggcgctcaac caggggatta ttgttgatat gtcccgccat atgaaccgca tcatcgaaat
     6841 taaccctgaa gagggctggg tgcgcgttga ggccggggtg ataaaagacc aactcaatca
     6901 gtacctgaaa ccgttcggct acttttttgc gccggaactt tcgaccagca accgggcaac
     6961 gctcggtggg atgatcaata ccgatgcatc cggtcaggga tcgctggtct atggcaaaac
     7021 gtcagatcac gtacttggcg tacgcgcggt gttgttgggg ggcgatattc tcgatacgca
     7081 acctttaccc gtcgagctgg cggaaacgct gggtaaatcc aataccacaa tcgggcgaat
     7141 ttataacacg gtttatcaac gttgccgtca gcaacgccag ttaatcatcg acaacttccc
     7201 caaacttaac cgctttctta ccggttacga tctgcgtcat gtctttaacg atgagatgac
     7261 cgagttcgac ctgacgcgca ttctgacggg ttcagaaggg acgctggcct ttattaccga
     7321 agcgcggctg gatattacgc gcttgcctaa agtgcgccgt ctggtgaatg tcaaatatga
     7381 ctcttttgac tctgcgctgc gtaacgcgcc gtttatggtt gaggcgcggg cgctttcggt
     7441 agagacggtg gactcaaaag tgctgaatct ggcgcgggaa gatattgtct ggcattccgt
     7501 cagcgagttg attaccgatg tgcctgacaa agagatgctc ggactgaaca ttgtggaatt
     7561 tgctggtgat gatgaggcgc tgattgacga gcgagttaat gcactctgtg tgcggcttga
     7621 tgagctgatt gctagccaac aggcaggtgt gattggctgg caggtgtgcc gcgagctggc
     7681 gggggttgaa cgtatctatg cgatgcgcaa aaaagccgtt ggtctgcttg gcaatgccaa
     7741 aggtgccgct aagccaattc cgtttgctga ggatacctgc gtaccgccgg aacatctggc
     7801 cgattatatt gctgaatttc gcgcgctgct cgacagccac ggcttaagct acggtatgtt
     7861 cggtcacgtc gacgcaggtg tcttgcacgt ccgtccggca ctggatatgt gcgatccgca
     7921 acaagagatt ttgatgaagc aaatctctga tgacgtggtg gcgctgactg cgaaatacgg
     7981 tggtttgttg tggggcgagc acggcaaagg ttttcgcgct gaatacagcc cggcgttttt
     8041 cggtgaggaa ctttttgcag aactgcgcaa agtgaaagcg gcatttgacc cgcataaccg
     8101 actcaaccca gggaagattt gcccgccaga aggtctcgat gcgccgatga tgaaagtgga
     8161 cgcggtgaag cgcggtacat tcgatcggca gatccccatt gcggtacgcc agcagtggcg
     8221 cggtgcgatg gagtgtaacg gcaacggttt atgcttcaac tttgatgccc gtagtccgat
     8281 gtgtccgtcg atgaagatca cccagaaccg gattcattca ccgaaagggc gcgcaacgct
     8341 ggtgcgtgaa tggctgcgct tgttggcgga tcgcggcgtt gacccactca aactggaaca
     8401 agaactgcct gaatccggcg tcagtttgcg gacgttaatt gcccgcacgc gcaatagctg
     8461 gcatgcgaat aaaggcgaat atgacttctc acacgaagtc aaagaggcga tgtcgggctg
     8521 tctggcctgt aaagcttgtt cgacccagtg ccccatcaaa attgatgtgc cggagtttcg
     8581 ctctcgtttt ctgcagctct atcacacccg ttatttacgc ccgcttcgcg accacctcgt
     8641 tgctacggtc gagagctacg caccgctgat ggcacgcgcg ccgaaaacct ttaacttctt
     8701 cattaaccag ccgctggtgc gcaaactctc ggaaaaacat atcggcatgg ttgatttgcc
     8761 actgctgtcg gtcccctcgc tacaacaaca aatggtgggg catcgctcgg caaacatgac
     8821 gctggaacag cttgaagctc tcaatgcaga gcagaaagcg cgcacggtgt tggtggtgca
     8881 ggaccccttt accagctatt acgatgcgca agtggtggcg gattttgtcc gtctggttga
     8941 aaaattaggt ttccagcctg tgttactgcc attttcgcca aatggcaaag cccagcatat
     9001 caaaggcttt cttaatcgtt ttgcgaagac ggcgaaaaag acggcggatt tcctcaaccg
     9061 tatggctaag cttgggatgc caatggtggg cgtcgatccg gcgctggtac tttgttatcg
     9121 cgatgaatat aaactggcgc tgggcgagga acgtggcgag tttaacgtct tacttgcgaa
     9181 tgaatggctg gcaagcgcac ttgagtcaca tccagtggct acagtcagcg gtgaatcatg
     9241 gtatttcttt ggtcactgta ccgaggttac cgctttgccg ggcgcgccag cacaatgggc
     9301 cgcgatattt gcccgttttg gcgcgaaact ggaaaatgtc agcgtgggtt gctgcggcat
     9361 ggcagggact tacggacatg aagcgaaaaa ccatgaaaat tcgctcggga tctatgagtt
     9421 atcctggcat caggctatgc agcgactgcc gcgtaaccgc tgtctggcga ccggatattc
     9481 ctgccgtagc caggtaaaac gggttgaagg cacgggggta cgccatcctg tgcaggcttt
     9541 actggagatt attaaatgat atggaaacgg aaaatcaccc tggaagcact gaatgctatg
     9601 ggtgaaggaa acatggtggg gttcctggat attcgctttg aacatattgg tgatgacacc
     9661 cttgaagcga caatgccagt agactcgcgg acaaagcagc ctttcgggtt gctgcatgga
     9721 ggagcatccg tggtactggc cgaaagtatc ggttccgttg ccggttattt atgtaccgaa
     9781 ggtgagcaaa aagtggttgg tctggaaatc aatgctaatc acgtccgctc ggcacgagaa
     9841 gggcgggtgc gcggcatatg taaaccgttg catctcggtt cgcgtcacca ggtctggcag
     9901 attgaaatct tcgatgagaa agggcgtttg tgctgttcgt cacgattgac gaccgccatt
     9961 ttgtgacgtt gtcaccagaa aagtgtgacg catattgctt ttggtcatta tctgcacagt
    10021 aaacggtggt atactgtgca ggtcttgtct aaaagcgagg atgctatgtc tactcaactt
    10081 gatcccaccc agctggccat tgaattttta cgccgtgacc agagcaatct ttcacctgcc
    10141 caatatctca aacgtctgaa acaactagaa cttgaatttg ctgacctgct tactctctct
    10201 tctgctgaat taaaagaaga aatctacttt gcctggcgtc tgggcgtgca ttgatcctga
    10261 cttaatgccg caagttcgcc aaatcgacct ggattaacct ccgtccaggt tcgatgacct
    10321 tcttaatact gcttatctac cgcctgatat tgttcaaaaa taatactaat cgcagtttgt
    10381 gttaaaacgg cgggttagct ttatgagatt attcttatcg ccccttcaag agctaagcca
    10441 ctgagagtgc cggagataag cgccggatgg ggtagaaacc cttaagcctg tgtcgcacag
    10501 acttaagggt tttcttattt ctgaacgctg cactttttta cctatctctt aaatagtcat
    10561 tttcaataca actttttctt tttcatcaca gccttaatgc tccttagata ataaccatta
    10621 tctaacaatg agatacctaa ttcttagcgt gcctgttaac ccacacatca ggggctatgc
    10681 ttattaaaag caggcaaaaa gcatgtttta cggtaaagcc cctgcgtttg ctgggttgaa
    10741 ctgataatca ttatcactaa catgctgtta tacgctgaaa gcgatgaagt gaggtaaata
    10801 gatggacatg cattcaggaa cctttaaccc acaggatttc gcctggcaag gcttaacgct
    10861 gacacccgca gcggcgatac acatccgcga gctggtggca aagcagccag gtatggtcgg
    10921 cgtgcgctta ggtgtgaagc aaacgggctg cgcgggcttt ggctatgtgc tcgacagtgt
    10981 tagcgagccg gacaaagacg atctgctgtt tgaacacgac ggcgcgaagc tgtttgtccc
    11041 gctgcaatcg atgccgttta ttgatggcac ggaaatcgat ttcgttcgtg aaggacttaa
    11101 tcagatattc aaatttcaca accctaaagc ccagaatgaa tgtggctgtg gcgaaagctt
    11161 tagggtatag gcggtactat gtctcgtaat actgaagcaa ctgacgatgt caaaacctgg
    11221 accggcggcc cgctgaatta taaagaagga ttcttcaccc agttagccac cgatgagctg
    11281 gcaaagggga taaacgaaga ggtggtgcgc gcaatttcgg cgaagcgtaa tgagccggag
    11341 tggatgctgg agtttcgtct aaacgcctat cgcgcatggc tggagatgga agaaccgcac
    11401 tggttgaaag cgcactacga caagctgaat tatcaggatt acagctacta ctcagcacca
    11461 tcgtgcggta attgtgacga cacttgcgcg tctgaacctg gcgcggtgca gcaaactggc
    11521 gcgaacgcct ttttaagtaa agaggtggag gcggcgtttg agcagttggg cgttcccgtg
    11581 cgggaaggca aagaggtggc ggtggatgcc attttcgact ctgtttcggt tgccaccact
    11641 tatcgcgaaa aactggcgga gcagggaatt attttctgtt cctttggtga ggcgatccac
    11701 gatcacccgg aactggtgcg taaatatctc ggcaccgtgg tgccggggaa tgacaacttc
    11761 tttgcagcac ttaatgcggc ggtagcctct gacggtacgt ttatttatgt gcctaaaggc
    11821 gtgcgctgcc cgatggaact ttccacctat tttcgcatta acgcggaaaa aaccgggcag
    11881 tttgagcgca caattctggt ggccgacgaa gacagctacg tcagctacat tgaaggctgt
    11941 tccgctccgg tgcgtgacag ctatcagttg cacgcggcag tggtggaagt catcatccat
    12001 aaaaacgccg aggtgaaata ttccacggta caaaactggt tccctggcga taacaacacc
    12061 ggcggtattc tcaacttcgt caccaagcgt gctttgtgcg aaggcgaaaa cagcaaaatg
    12121 tcatggacgc aatcagaaac cgggtcagcg attacgtgga aatatcccag ctgcattttg
    12181 cgcggcgata actccattgg tgagttttac tcagtggcgc tgaccagcgg tcatcagcaa
    12241 gcggataccg gcaccaagat gatccacatc ggtaaaaaca ccaaatcgac cattatctcg
    12301 aaagggatct ctgccggaca tagtcagaac agttatcgcg gcttagtgaa aatcatgccg
    12361 acggcaacca atgcgcgcaa tttcactcag tgcgactcaa tgctgattgg cgctaattgt
    12421 ggggcgcata ccttcccgta tgtcgaatgt cgcaataaca gcgcacaact ggagcacgag
    12481 gcaacgacat cacgtattgg tgaagatcaa ctgttttact gcctgcaacg cgggatcagc
    12541 gaagaagacg ccatctcgat gattgttaac ggtttctgca aagacgtgtt ctcagaactg
    12601 ccgctggaat ttgccgttga agcacaaaaa ctcctcgcca tcagtcttga acacagcgtc
    12661 ggataaggaa taaacatgtt aagtattaaa gatttacacg tcagcgtgga agataaagct
    12721 atcctgcgtg gattaagcct cgacgttcgt cccggcgaag ttcacgccat tatggggcca
    12781 aacggttcgg gcaaaagtac tttatcggca acgcttgccg ggcgcgaaga ttatgaagtg
    12841 acgggcggca cggttgagtt caaaggcaaa gatttgcttg cactgtcgcc ggaagatcgt
    12901 gcaggcgaag gcatctttat ggccttccag tatccggtgg agattccagg cgtcagtaac
    12961 cagtttttcc tgcaaacggc acttaatgca gtgcgcagct atcgcggcca ggaaacgcac
    13021 gaccgctttg attttcagga tttgatggaa gagaaaatcg ctctcctgaa gatgccggaa
    13081 gatttattaa cccgttcggt aaacgttggt ttttccggcg gcgagaaaaa gcgcaacgat
    13141 attttgcaaa tggcggtgct ggaaccggag ttatgcattc ttgatgagtc ggactccggg
    13201 ctggatattg acgcattaaa agtggtcgcc gatggcgtga actcgctgcg tgatggcaag
    13261 cgctcattca tcattgttac gcactaccaa cgcattctcg actacatcaa gcctgattac
    13321 gttcatgtgc tatatcaggg acgaattgtg aaatccggcg atttcacgtt ggtcaaacaa
    13381 ctggaggagc agggttatgg ctggcttacc gaacagcagt aatgcgctgc aacagtggca
    13441 tcacttgttt gaagctgaag ggacaaaacg ctcaccgcaa gcacagcagc atttacaaca
    13501 attgctgcgt accggactgc cgacacgtaa acatgaaaac tggaaatata cgccgctgga
    13561 agggctgacc aatagtcagt ttgtcagcat tgcgggagat atatccccac agcagcgtga
    13621 tgccttagcg ttaacgttag acgccgtgcg gctggtgttt gtcgatggac gttacgtgcc
    13681 cgcgctgagc gatgcaattg aaggcagcgg atatgaagtg agcattaacg acgaccgtca
    13741 ggggttaccc gacgctattc aggcggaagt gtttctgcat ttgacggaaa gcctggcaca
    13801 aagcgtgacg catatcgccg tgaagcgcgg tcaacggccg gcaaagccat tgctgttaat
    13861 gcatatcacc cagggcgtgg caggtgaaga ggtgaacact gcccattacc ggcatcatct
    13921 ggatctggcg gaaggtgccg aagcaacggt gatcgaacat tttgtcagcc ttaatgatgc
    13981 tcgtcatttt accggggcac ggttcactat caatgtcgca gcgaacgccc acttgcagca
    14041 tatcaagctg gcgtttgaaa acccggtcag tcaccacttt gcccataacg atttgttgct
    14101 ggctgacgat gccaccgcat ttagccatag tttcctgctg ggtggcgcag tgttacgaca
    14161 caacaccagt acgcaactca atggcgaaaa cagcacgctg cggatcaata gcctggcgat
    14221 gccggtgaaa aacgaggtgt gtgatacccg tacctggctg gaacacaata aaggtttttg
    14281 taacagccga cagttgcaca aaactatcgt cagcgacaaa ggccgcgcgg tatttaacgg
    14341 tttgatcaac gtcgcgcagc acgccatcaa aacggatggt cagatgacca acaataatct
    14401 gctgatgggc aaactggcgg aagtggatac aaaaccgcag ctggaaatct atgcagatga
    14461 tgtgaaatgc agccacggcg cgacggtggg gcgtattgat gatgaacaga tgttctatct
    14521 gcgctcgcgc gggatcaatc agcaggatgc ccagcagatg atcatttacg cctttgctgc
    14581 cgaactgacg gaagcactgc gtgatgaggg gcttaaacag caggtgctgg cccgaatcgg
    14641 tcaacggctg ccaggaggtg caagatgact ttttccgtcg acaaagtgcg ggccgacttt
    14701 ccggtgcttt cgcgtgaggt aaacggtttg ccgctggctt atctcgacag cgccgccagt
    14761 gcacagaaac cgagccaggt gattgacgcc gaggccgagt tttatcgtca tggctacgcg
    14821 gcggtgcatc gcggtattca taccttaagc gcccaggcga ccgagaaaat ggagaacgtg
    14881 cgcaagcggg catcgctgtt tattaatgcc cgttcggcgg aagagctggt gttcgtccgc
    14941 ggcacgacgg aagggatcaa tctggtcgcc aatagctggg gcaacagcaa tgtgcgggcg
    15001 ggcgataaca tcatcatcag tcagatggag caccacgcta acattgtccc ctggcagatg
    15061 ctttgcgcac gcgttggcgc agagctgcgt gtgatccctc tcaaccccga cggtacgctg
    15121 caactggaga cgctgcctac gctgtttgat gagaaaactc gcctgctggc aattactcat
    15181 gtctccaacg tgcttggcac agaaaatcca ctggcggaaa tgatcacgct tgcgcaccag
    15241 catggcgcaa aagtgctggt ggatggcgct caggcggtga tgcatcatcc ggtggatgtt
    15301 caggcgcagg gttgcgactt ttacgtgttc tccgggcata aactgtatgg ccccaccgga
    15361 attggcattc tttatgtgaa agaagccttg ttgcaggaga tgccgccgtg ggaagggggc
    15421 ggttctatga tcgccaccgt cagcctgagt gaaggcacta cctggaccaa agcaccatgg
    15481 cggtttgaag ccggtacacc caataccggg ggcatcattg gtcttggcgc ggcgctggag
    15541 tatgtttcgg cgctggggct taatagcata gccgagtatg aacagaatct gatgcattat
    15601 gcgctatcac agctggaatc tgtaccggat ctcactctct atggccaaca aaacaggctt
    15661 ggcgttattg cttttaatct cggtaaacac cacgcctatg atgttggcag ttttctcgat
    15721 aattacggca ttgctgtgcg taccggacat cactgcgcta tgccattaat ggcctattac
    15781 aacgtccctg cgatgtgtcg ggcgtcgctg gccatgtata acacccatga agaagtggat
    15841 cgtctggtga ccggcctgca acgtattcac cgtctgctgg gataacaggg aggcactatg
    15901 gctttattgc cggataaaga aaaattgctg cgtaattttt tacgctgcgc caactgggaa
    15961 gagaaatatc tctacattat tgagctgggc cagcgtctgc cagaattacg cgccgaagac
    16021 ggaagcccac aaaatagcat tcagggctgc cagagtcagg tgtggattgt catgcgccag
    16081 aatgcgcagg gaattattga attgcagggc gacagcgatg cggcgattgt gaaagggctt
    16141 attgcggtcg tctttattct ctacgatcag atgacgccgc aggatattgt caatttcgat
    16201 gtgcgtccgt ggtttgaaaa aatggcgctc acccaacatc tcaccccatc tcgttcacaa
    16261 ggtctggaag cgatgattcg cgcaattcgc gccaaagccg ctgcacttag ctaaactaaa
    16321 gggacagctt tcatccggtt ggctcacatt gccggatggt caatgtttac actgacctgc
    16381 acaggagtgt tttggtttca ggtgaacata agcaccatcc gacgtttcag cgtgagtctc
    16441 cggcaaatac aggaggttta caatgaaacg cgcgtctttg cttacactca cgcttatcgg
    16501 cgcttttagc gccatccagg ctgcctgggc ggttgattat ccgctaccac caaccggaag
    16561 ccgactggtt gggcaaaatc aaacgtatac ggtgcaagaa ggggataaaa accttcaggc
    16621 tattgcccga cgttttgata ctgcggcaat gttgatcctt gaagccaata acactatcgc
    16681 cccggtgcca aaacctggta cgacgataac tattccttca caactgttat tacctgatgc
    16741 accgcgtcag gggattatcg ttaaccttgc agagctgcgc ctttattatt atccgccggg
    16801 agaaaatatt gtgcaggttt atccaatagg tattggattg caggggctgg aaacgccggt
    16861 gatggaaacg cgtgttgggc agaaaatccc taacccaacc tggacgccta cggcaggcat
    16921 tcgtcagcgt tcgctggagc gtggcattaa attaccgcca gtcgttcctg ccggaccaaa
    16981 taacccgcta ggacgttacg cactgcgcct cgcgcatggt aatggcgaat acctcattca
    17041 tggcaccagt gcgccggaca gcgttggttt gcgcgtcagt tcagggtgta ttcgcatgaa
    17101 tgcaccggat attaaagcct tgttctccag cgtgcggacg ggaacgccgg tgaaagtgat
    17161 caacgaaccg gtgaaatatt ccgtggaacc taacgggatg cgttatgttg aagtacatcg
    17221 accactatca gcagaagaac agcaaaacgt tcagacaatg ccatacacac tgccagcagg
    17281 ctttacgcaa tttaaagaca ataaggctgt agatcagaag ttagtcgata aagcgttgta
    17341 tcgtcgggca gggtatccgg tttcggtgag cagtggagca actcccgcag ccagcaatgc
    17401 gccttcagta gagtcagcgc agaatggtga accagagcaa gggaatatgt tacgcgcgac
    17461 gcagtagtgg taaatagcag acaaaaaaaa tggcgcacaa tgtgcgccat ttttcacttc
    17521 acaggtacta ttacttgcgg tatttagtag ccatgttgtc cagacgctgg ttagcacgag
    17581 ctgcgtcatc tttagcagcc tgaacgtcgg aacgcattgc gttcacgtcg ttgctcagct
    17641 ggtcaacttt agcgttcaga gtctgaacgt cagaagacag ctgatcgatt ttagcgttgc
    17701 tggagcaacc tgccagcaga gtagaaccca ggattaccgc gcccagtacc agtttagtag
    17761 ctttcattat taataccctc tagattgagt taatctccat gtagcgttac aagtattaca
    17821 caaagttttt t
//
LOCUS       DAJVQH010000077        17389 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJVQH010000077 DAJVQH010000000
VERSION     DAJVQH010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.077"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..1139)
                     /locus_tag="OQM87_002651"
     CDS             complement(<1..1139)
                     /locus_tag="OQM87_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1508733.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADDIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPSCVVPKLNSSWEPVYQVTFPDGSADVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGG"
     gene            complement(1139..1321)
                     /locus_tag="OQM87_002652"
     CDS             complement(1139..1321)
                     /locus_tag="OQM87_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508734.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            complement(1333..2829)
                     /locus_tag="OQM87_002653"
                     /pseudo
     CDS             complement(1333..2829)
                     /locus_tag="OQM87_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(3063..3557)
                     /locus_tag="OQM87_002654"
     CDS             complement(3063..3557)
                     /locus_tag="OQM87_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCS1508735.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEELPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3683..4033)
                     /locus_tag="OQM87_002655"
     CDS             complement(3683..4033)
                     /locus_tag="OQM87_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1508736.1"
                     /translation="MPPRIPKACRVRGCRSTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDVIRVRVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKEKTARERLK"
     gene            complement(4084..4416)
                     /locus_tag="OQM87_002656"
     CDS             complement(4084..4416)
                     /locus_tag="OQM87_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508737.1"
                     /translation="MPVNIDPEKLNDEREQVIAKWLFKDVDLISQQIELGEENVKRFD
                     ELLSIFDCCQSSWFATEHLFDNTELEKVWHEFESNFNKYINGGESKDLLMKMLDKLIS
                     SRFVFESR"
     gene            complement(4879..5271)
                     /locus_tag="OQM87_002657"
     CDS             complement(4879..5271)
                     /locus_tag="OQM87_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCS1508738.1"
                     /translation="MKLSYKLLIVGFFVTVIGSFIWSANHYYSKYQYEKKRADEAVRN
                     AESATAITNNVLQSLQIVNTVLEANQHAKQQIALESQRAREDIKVAVADDDCASRPVP
                     DVAADRLRKYADSIRAGSNDAVTDEPAR"
     gene            complement(5255..5731)
                     /locus_tag="OQM87_002658"
     CDS             complement(5255..5731)
                     /locus_tag="OQM87_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCS1508739.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLALKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKEAGGVVNEAEL"
     gene            complement(5735..6061)
                     /locus_tag="OQM87_002659"
     CDS             complement(5735..6061)
                     /locus_tag="OQM87_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCS1508740.1"
                     /translation="MPHNPNTWLELVQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGGSKE"
     gene            6358..7689
                     /locus_tag="OQM87_002660"
     CDS             6358..7689
                     /locus_tag="OQM87_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HCS1508741.1"
                     /translation="MLDTLSFTERDEFQRRNIAENIIKLLKPEADISPLVIDGAWGTG
                     KTEFSIKLKNLIIEQETESKVVYIDAFKGDHAESPLLLITSAIASILPEEEKQHFIKR
                     SLPAIRFGLKTVLKAGAGWFLRQEASEVSEEFQDAMKKASNAAIDGTIENLLEDHMES
                     EKNINSLKSCIESISESQKIVIIIDELDRCKPSFSTNVIETIKHIFDINNVFFILVTN
                     TEQLKASINHIYGYSINSQKYLDKFIKYTITLPDTCLINGHVVCKTSVIYWNYLVDKT
                     KSLNEINRLSGRFIRDLIQRTNLSLRETQTFSRNLNIFQLLNDDEYKSSDPLINKIFV
                     VAVFIHCFGDKEKLKQEITAESISHLADLLNIKEIPYSYERRSQIPEISIVFFGIIKD
                     SITLNERFAPKSDEELKKFTTVYTDYEHLNFWSTTPRELLIKYINQMSFIQ"
     gene            complement(7718..8086)
                     /locus_tag="OQM87_002661"
     CDS             complement(7718..8086)
                     /locus_tag="OQM87_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HCS1508742.1"
                     /translation="MMRDIQMVLERWGAWAASDSSGVDYSPIAAGFKGLLPYTSKKRL
                     ACSDSDALIIEGCLARLKQKRPYEHSLLVAHYLYRISKRKIAKARGKDEKLIRIEIQL
                     AEGFIDGCLSMLDLTLDLDV"
     gene            complement(8101..9090)
                     /locus_tag="OQM87_002662"
     CDS             complement(8101..9090)
                     /locus_tag="OQM87_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1508743.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(9098..9895)
                     /locus_tag="OQM87_002663"
     CDS             complement(9098..9895)
                     /locus_tag="OQM87_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCS1508744.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSQDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVSRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(9915..10304)
                     /locus_tag="OQM87_002664"
     CDS             complement(9915..10304)
                     /locus_tag="OQM87_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1508745.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITINKYEKTAGAAA"
     gene            complement(10301..10627)
                     /locus_tag="OQM87_002665"
     CDS             complement(10301..10627)
                     /locus_tag="OQM87_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCS1508746.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRATHWLH
                     ERDLKV"
     gene            complement(10624..11277)
                     /locus_tag="OQM87_002666"
     CDS             complement(10624..11277)
                     /locus_tag="OQM87_002666"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCS1508747.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQVLTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(11277..11771)
                     /locus_tag="OQM87_002667"
     CDS             complement(11277..11771)
                     /locus_tag="OQM87_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCS1508748.1"
                     /translation="MSMSLLNDVQKFIEAHRGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(11768..12709)
                     /locus_tag="OQM87_002668"
     CDS             complement(11768..12709)
                     /locus_tag="OQM87_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1508749.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTAEQEFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(12699..12878)
                     /locus_tag="OQM87_002669"
     CDS             complement(12699..12878)
                     /locus_tag="OQM87_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1508750.1"
                     /translation="MRQVNRWFKEHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(13054..13605)
                     /locus_tag="OQM87_002670"
     CDS             complement(13054..13605)
                     /locus_tag="OQM87_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508751.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDEQYLSISKLQEHAALVYKIFCVSE
                     SSDARECAAPGAVACRDCGETNA"
     gene            complement(13649..13849)
                     /locus_tag="OQM87_002671"
     CDS             complement(13649..13849)
                     /locus_tag="OQM87_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1508752.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            13940..14614
                     /locus_tag="OQM87_002672"
     CDS             13940..14614
                     /locus_tag="OQM87_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HCS1508753.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            14787..15083
                     /locus_tag="OQM87_002673"
     CDS             14787..15083
                     /locus_tag="OQM87_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508754.1"
                     /translation="MLKQVYEMKVQILNNNCEVVWSYDIAAPVDQSGDSWTNGKHQIM
                     AGVVFSLRRALEQAEVFPSDPEWKWPFSICPNSESTFQKIGQKVALEEHQPTVS"
     gene            15359..15669
                     /locus_tag="OQM87_002674"
                     /pseudo
     CDS             15359..15669
                     /locus_tag="OQM87_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15666..16046
                     /locus_tag="OQM87_002675"
     CDS             15666..16046
                     /locus_tag="OQM87_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508755.1"
                     /translation="MMRSSKMASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMN
                     TKTKGKVSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTI
                     LQEDQGQLLTLAGEPDGKLRAAGH"
     gene            16112..16936
                     /locus_tag="OQM87_002676"
     CDS             16112..16936
                     /locus_tag="OQM87_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HCS1508756.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCIPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            17064..>17389
                     /gene="yfdR"
                     /locus_tag="OQM87_002677"
     CDS             17064..>17389
                     /gene="yfdR"
                     /locus_tag="OQM87_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCS1508757.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDSVIR
                     EKYGLPP"
BASE COUNT         4460 a         4566 c         3965 g         4398 t
ORIGIN      
        1 ccaccgggac gcggattcat atcttccagg tcgcggcagt cattgggaga gtaaattccc
       61 cagttaatcc cggtggcgta ggcttcaaaa cgggacttca tatccccgcg cagtaacgcc
      121 ccggcgttaa atttggcgta ataaacgccc tgcttacttt ttcgtaccag tccggtgttg
      181 atccgctgtt cgatgcgggt cagatacggc accagtgaat agttgataaa tcccagcccc
      241 agctcttcaa tattgttgaa agtggcgcga tcggtgttct gcaccatgtg caacggcacc
      301 cggaacagac gacagatttc ttcaagctga aacttgcggg tttccaggaa ctggctgtcc
      361 tcggcgttca gcgccatcga cttccagtcc agccccatct caaggatcat cgggcggtga
      421 gcattgccaa gcccggtgtg acgctcctca aaatctttct tcaggcgctc gtaagcctga
      481 tctgacagcg tctgctctgt acgcaacaca cccgacgtca ccgcgccatt gctgaacagt
      541 ctggccccgt gctcttcggt cgctgccgcc agcgatattg cctcgcgggc ataggcgatg
      601 ggattcagcc ccaccagtcc gtccagcgtc agcgtgcgca catgccagat atcctcctgg
      661 ctcagtacat ccgcggagcc atccgggaat gtgacctgat agaccggctc ccagctactg
      721 ttaagcttcg gtaccacaca gctgggatcg acgggcagca gttcagccac ttcgccaaat
      781 gctttcactt tgtaggcgta aaagtttccc cgcaggcaca gacaggtgac caccagctcc
      841 cagaactcct gcggcgtcat atagccattg ggatgcgtgg agatcagctt atgcagacgt
      901 tcgccagtgg ctctctgctt caggctgccg ttcaggtgat acaggttgca gggcaacatc
      961 ccgaccgact ccgccagcac cctgacacag gaaaaaaccg ccgtcagtcg catggcccgc
     1021 tggctgctga tctgctttcc ggtataggtg tcgtaggaca acccgatatc atccgccagc
     1081 tctgctggcg tggtcaccgg tgcgtcactt tttcgttgaa ataatcccga aaagaacact
     1141 atttacctcc gccgacagac gactgtgtac ggtcgagata tcgcgccacc agccacgacc
     1201 agaacaggca caacgccccg gcaacaacaa accccgccgg gggataaatc agccaggcac
     1261 catacgccag caaaagcgcc cccagcacgc ccaccagagg cgcgagaatc agcatgatca
     1321 taattacctc agttaaagcg agcggatccc ataggactca atgtggtcag acagcgtgtc
     1381 ttctttctcg tacagcatgg ctctgccaac cgccataatc agcgcaactg caccgtcaat
     1441 tttgttttcc gcctgctctt tgacgggctt caccacatca tcgttacccg gaatggtttt
     1501 gccgaccacg ttgccgatac actaggtcat gatgggattg ccatcatgat gaaagcgccc
     1561 cgattcaatt gccgcttcca gctctttcat cgggtcggac atgttggtgt agttctgaat
     1621 gatagtgatg gggttcaggt cttcatcagc aaggtcatgt gacaacccgg tcgccccgaa
     1681 ggggtcgatg ggtgactcac tgaccggact gattttgttc gccgctttgg cctcctcgag
     1741 gatgtagcga taatccacct ccgcaccatc ggtaacggtc agaacgccca tttccaccca
     1801 tttctgaaag cgttcggctg tccgtcgatc ttcatttttc tcgacgctgt acaccgtgtc
     1861 atacggtacc cagaaacgcg gggccacact gtagtaatgc gttttaccgt caatctcgcg
     1921 ggtataaagt cgcgccatgc tgttcatatc cagcttacgc gccaggtcaa aggccagaat
     1981 gcacggctgc ccctcgaact gctcaagggt cagtgattta tcctcgcagc tctgccagct
     2041 caccaggttg aaatacgccg aacgcgccga cacccagata ttgaggtgtt ttgttttaaa
     2101 gacgtttgcc agacgggcgt tatttttcgc acgctgctgc tgacttaaca aaaattcgcg
     2161 ataaaccgac acgccaatat ttggattggc tttttccagc acctgcgggt cggtccagtc
     2221 gtcaccttca tcaacggtat agataatccc gaacagttca tcgttgggta ccgaaccgtt
     2281 gagcatctcg atgacttccc gccgtttgtc gtagcacggc ccctcaatgt tgtacccggc
     2341 ggtagtgata gcccacatca gtggctgacg tcgcgccccc atcccggtaa gcatcgtggt
     2401 gtaaagcgca tctgtggcgt gctcgtgata ttcatccacc accgcacagt ggggtgatga
     2461 accatcaccg gggttaccaa tcagcggttc aaaccgcgcc ccatcctccg gacggttcat
     2521 gtttgaggcg ttaacctcaa tcccgaacgc ttccgtcagc atgggtgtgc gtttacacat
     2581 cagtcgcgcc gggcgaaaga cttcccacgt ctgtttctct gtcgtggcac cggaatacac
     2641 ttccgcgcca aactcgttat cacaggcaaa acaatacagg gcaacaccgg cagagattgc
     2701 cgatttgccg ttcttacggg ggatttcggt atacacctcc cggaagcggc gcagccggga
     2761 ccctttattg acccagccaa acgcacagca gatcacaaag agctgccacg gctccagcgt
     2821 gatgggcatc ctcttgaatg cccactcccc cttggtgtgt ggcaacagct gaataaattt
     2881 cgcggcccgt tcagccagct ccttgtcgaa gcggtaacga aacgacttac ttttttccgc
     2941 catcaggtca tcaagatggc gctggcaggc ctgaatcaca aactggcagg ccacaatctt
     3001 tccgcgcacg acatcacggg catactgatt ggcagcattt acgttggggt aagatttccg
     3061 gctcatgact cgatgatttt cagaaacggg ttagtggctt tcttctgccc cgccaggcca
     3121 atcagacgct ggcggctact ggggtcgagt ccgagcattg cccccgtgct gctcatctcg
     3181 gactcctgtt cttttttggc ggtcagctcc ggatttttga ccataccgcc cattgcaccg
     3241 gtgatggtgt tgccctgtct ggcaatattt ttcacggcac gccgccagaa ctcataggcc
     3301 acacaccacc gctcaagcac cgcaaggtca gtcacgcaca gcaggccctg accgcagagt
     3361 tctttggttg tcagttgcca catgatcgtg gcgagaggaa gctcttcttc agcgaaccac
     3421 tccggtggct caacaccttt gatgggcgta aaaacaggtt catctttgtt cagggctcgc
     3481 ttgccggggt ttccggccag cgccttgcgc gccgttggct tagggcgacg cccggaacgc
     3541 cccgccgttc cagccatatg cggcactcct ggttaaattt catttttcgc gggtataaaa
     3601 aaacgatggg gcgggcagtc cggaagacgt caggtcacag agatttgacc cgcccctccc
     3661 ctcagacagt tgagaattat gatcacttta gccgttcacg ggccgtcttc tccttatggc
     3721 acggccagca caggctctgc agattgctgt cggcatctgt gccgccatgc gctttaggga
     3781 tgatgtggtc aacagttttc gcctcacgca ccacaccagc acgcagacat agctgacaca
     3841 ggcctttgtc acgcttcagg acacgcacgc ggataacatc ccacttcgaa ccataaccgc
     3901 gctgatgacg agattgtcct ggcttgtatt gcttccagcc ttcgctttta tggctttcgc
     3961 agtagcctga cgggtccgtg gttgtagagc ggcagccgcg aacacggcag gcttttggga
     4021 ttctaggggg catataaaaa atttataata agtaaaataa taaagaaaaa tactcaaagg
     4081 caattatctt gactcaaata caaatctaga agagatgagc ttatcaagca ttttcataag
     4141 taaatcctta ctctcaccac catttatata tttattaaag ttagactcga actcatgcca
     4201 aactttttct aactcagtat tatcaaataa gtgctctgtc gcaaaccatg aagactggca
     4261 acaatcgaaa atactcaaaa gttcatcaaa tcttttaaca ttctcttctc ccaactcaat
     4321 ttgctgacta ataagatcta catctttaaa tagccatttc gcaataactt gctctcgttc
     4381 atcattgagt ttctcggggt caatattgac aggcataaat aatagagctg tctttaattt
     4441 ctttaatgca ttcctaaaat ctatttttac ttttgttttc tcttgttccc gccacgtaaa
     4501 caatgccttg aacgccaaaa ccaaagtaac aatagtagct ccagcactca cccaagatgc
     4561 aatcattgcc caacaagccc actcagcagc agcacggttt gctacaagtg tctcatatgc
     4621 aatataattt tcgttcattt ttacctcact gttaatagtg agagtattgt aactaaatgc
     4681 ataataacta gaacagtcaa ataataatat ttcacctaat gaatattatt attcaaatgc
     4741 agattgtact tttgctctcc taacctacga agatcagact tatcacggtt acatagcccc
     4801 agtgctgata gcagacttac attcaaacta agactatccc cataagtcag aggattgggt
     4861 ataactggct gtggagtttc agcgagcagg ttcgtcggta acggcatcgt tggaacctgc
     4921 acgtatactg tccgcgtact tccgcaaccg gtcagcagcg acatcaggca cagggcgtga
     4981 agcacaatca tcatccgcaa cagccacttt gatatcttcc cgggctctct gtgactccag
     5041 tgcgatctgc tgttttgcat gctggttagc ctccagaact gtattgacga tttgtagtga
     5101 ttgcaggacg ttattggtaa tggcagttgc tgattcagca tttcgtacag cctcatcagc
     5161 acgtttcttt tcgtactgat atttgctgta gtagtggttg gccgaccaga tgaaagaacc
     5221 aatgacagta acgaagaaac caacgataag cagcttatag ctcagcttca tttaccaccc
     5281 caccagcctc tttaaaccgg gcaatcaaat caccgatttt atgttcatac tgaccataac
     5341 ctgcaccggg taacgacgcc cagatattgc tgcaacggtc gatagcctga cgaatatcac
     5401 cgcgatcaat catcggtaaa gcgccacgct ctttaatctg ttgcagtgcc acagcatcct
     5461 ggcttttggg ggagaagtct ttcagagcaa gctgcttacg gtaggcatcc caccagcgtg
     5521 aaagaagctg ataacgtccg gcggctgttg atttgagttt ggggtttagc gtgacaagtt
     5581 tgcgagggtg atcagagtaa tcagtaaaca gttcgccgcc aacaataaca tcataaccgt
     5641 gattacgtgt cggttgtcgc ccgttatccg ttccttctga ccatgccacc atatcgagga
     5701 aagctttacg ctgggaattt agtacctgca taaattactc cttagagcca ccaaacttat
     5761 taccgattac tctcattgca gccccacgaa tagcatcgac cccgattagc ccaacgccgc
     5821 caccaatggc aacagaaaga gatttaggcc atccgacata ctcaagagcg gatgcaaaag
     5881 tcagcgtcag agcaccacag agcaaaatct cgagcgtttt tcgtttccag cccccaccac
     5941 cgccaaaata tgcaatgcgc aagccagcca taacaatcga cataatcact gcgcccagcg
     6001 gtgtgtctcc acgccaccag ctctggacca actccaacca ggtatttggg ttatgaggca
     6061 tttgtagtta tctctcacct cgctgataca gcaggtgcaa attgagggaa catcatgtac
     6121 cgcaaatcag aagcggaaac gtcaaagagc cgaaccaatg gataactgcg ggataggcca
     6181 ggcccaacga atccccagca acaaaaacga caaaacccgc tcgacggcgg gtttaagctg
     6241 tgtggcgaag taaccactct taacagatta caagaatttt tgcggaccgc gttaatgatt
     6301 tttaatccca aagtcgtatt attcgttttt tactgtagga gttaataggt ttatattatg
     6361 ttagatacac tatctttcac tgaacgtgac gagttccaac gcagaaacat cgctgaaaat
     6421 atcatcaagt tgctaaaacc agaggcagac atttcaccac tggtaataga cggcgcatgg
     6481 ggaacaggga aaacagaatt tagtatcaaa ctgaaaaatc tcattattga gcaagaaact
     6541 gaatctaaag ttgtttatat tgatgccttt aaaggggatc atgcagaatc tccattactt
     6601 ctcataacct ctgcaattgc cagcatttta cctgaggaag aaaaacaaca cttcattaag
     6661 agatctcttc ctgcaattcg ctttggttta aaaacggtac taaaagctgg tgcaggttgg
     6721 tttttacgac aggaggctag tgaagtttcc gaagaattcc aagatgcgat gaagaaagca
     6781 agcaatgcag caatagatgg gactattgaa aatttgcttg aagaccatat ggaatcagag
     6841 aaaaacataa attcacttaa atcttgcatc gaaagcattt cagaaagtca aaagatagta
     6901 attataattg atgaattaga ccgatgtaag ccgagttttt caacaaatgt cattgaaaca
     6961 ataaaacaca tttttgacat caataatgtc ttttttattt tagttacaaa cacagaacaa
     7021 ttaaaagcat cgataaatca tatttatggt tatagtatta actcacaaaa gtatcttgac
     7081 aagtttataa aatataccat cacgcttcca gacacatgtt taataaatgg tcacgtcgta
     7141 tgtaaaacat ctgttatata ttggaattac cttgtagaca agacaaaatc attaaatgaa
     7201 attaataggt tatctggtag atttatccgt gatttaattc aacgaaccaa cttatcctta
     7261 cgtgaaacac aaactttttc acgcaatctt aacatttttc aactattaaa cgacgatgag
     7321 tacaaaagca gtgatccttt aataaataag atctttgtcg tagctgtctt catacattgc
     7381 tttggtgaca aggaaaaact aaaacaagaa attaccgcag aatctatatc tcatttagca
     7441 gatctgctta acataaaaga aataccttat tcttatgaga gaagatcgca aattcctgaa
     7501 atatctattg tattcttcgg aataattaaa gacagtatta ctcttaatga gcgatttgcc
     7561 cctaaaagtg atgaagaact taaaaaattc accactgttt atactgatta tgaacaccta
     7621 aatttttgga gtactacacc cagagagtta ttgataaaat atattaatca aatgtcattc
     7681 atccagtaaa taatacgccc ctgcatgggg cgtattatta aacgtccaaa tctaatgtta
     7741 gatctagcat ggaaaggcag ccatcaataa atccttcggc tagctgtatt tctatacgta
     7801 tcaatttctc atcctttcca cgcgcctttg caatcttacg cttggatatt ctgtataaat
     7861 aatgtgccac aagaagcgaa tgctcataag gtcttttttg ctttagacga gcaagacaac
     7921 cttcaataat taatgcatca ctatccgaac aagccaagcg tttcttgctg gtatagggaa
     7981 gaagtccctt aaacccagca gctataggtg aatagtcaac gcctgaacta tcactcgccg
     8041 cccatgctcc ccaacgctca agaaccatct gaatatcacg catcaacttt ctccaccaaa
     8101 tcaggccagc acgccaattg ccagcgcacg atcgataaaa cgaaatatca gctccagctg
     8161 ggagccatac ttctcttcaa atgccacggt atccgcatgc agctcgtcgt gatgctttct
     8221 gcacaaaggc aacacaaaga ggtcatgcgc ttttgtaccc attcccccct gaccgtggcc
     8281 tatcaggtgg tggggatcat cagcgggctt tccacaacat gcacacggct gtgtcttaac
     8341 ccagcgcgtg tacttctcat taacccagcg gcgacgtttt gggcgtaaca taaaagactc
     8401 cggcgactcc ggatccactt tcagcgccag cacctttttt gccttatctt ggatgatgct
     8461 ggtggcagga accgaaggca caaggtcact ttcccgggtg acagacggca caacaggctt
     8521 tggtaatctc agtgccttac gggctgcact ttccggtaag gcatccgcca ggtcattacg
     8581 aatcagccac cagcacagtt ccggcattgt cacaacgtga ctatcatcaa aaccgagatc
     8641 ccgacgcaca acagacaaca cccagcgggc acagttatcc gttgccattg attccagccg
     8701 ttccgtgaac tgatcgcgca gctggttatc acagtgccag cacagacgga ttgcgcccgg
     8761 agcgtgtcgc attgtggtca tgttctcgct gtgccagtcg gaatgaggcc actggcagcc
     8821 tttttcacga agtagccagc tttcaagaca ttccacgcca ccagcacgac ggatcactgc
     8881 ctcattgcgg aacacggccc gaacagcagg atcatccgcc agcggttgtg atgccgcggg
     8941 aacggcacca ctggcgaaag atgaataacg ttccggctca ggctccagca gtacacgccc
     9001 ctgcataaac aggggcatca gctctgaacc tggtctgaac aatacgatcc ccatacgcgg
     9061 ggcaatttca ggggtcagta gtgctctcac ggtcacctca atgaacggta tcgagcagct
     9121 ttaacagctc agggaatcgg gattcgaaga aatgcggctg cgtctcgcgc ggatttgcag
     9181 gactggtaat gttcttgccg aacatgcagc ctttcgccgt cagcgaccag aattttttga
     9241 tgttgttaat cgcggtacgg ctgtatcgtt cgcgttgttc aacgatcccc agcttcgcca
     9301 tctggtgata tgcctgatta gctgtcaggc ggataccata ctgtttcagc agtgcactca
     9361 gcgacagcgt agggcgactt gagccatcag gcgcgtcagc aggagcatca atggcatagc
     9421 gtggtgccag attcggtaag ccaacagcct cctggagttt ctgacaggcc ccaagcacag
     9481 atgagttaga caggtttaac tcccgccgca taaagtccag cagaatcacg ccagcctgca
     9541 tcttgtcagc agcctgcccg gataattttt ccggtgcgct ggttaccata tcgaaagtac
     9601 ggatcacctt cagatggaat gacgggctga tccacattgc ataggcatac accagttctt
     9661 tgcagacata cgtcccctgg ttatttccgc cacgactaac gttaactggc tctatattga
     9721 ccgagttgca aatctgcaac tcgcttatta aacgttcggt ttgctcattg cggagccaga
     9781 atgcaggctt atgcttatcc tgagaaccgg cagccctgtg cagatcgttc aggctgtaac
     9841 gcccataagc atcacgacga acttcaatac catcaatgac catcagatta ttcatacttc
     9901 gtttctcctc ttaatcaggc ggctgcaccc gccgttttct cgtacttatt gatagtgatc
     9961 tcgaccttcc cttccgggat aaccggcccc cactccacca gcattctttt cacctgactg
    10021 tcgtcttccc acacacccgc gtgggtcagg gcgtcaaaca gcgccttgtt atagttgtcc
    10081 agatcgcgga tccggttatc cggaggaaac aacacgatct ccactgaagc aggtgccgac
    10141 gttggtttcg gcagacgacg taactgctca actattgctg cgcacgccgc gctctggaat
    10201 tttcgccccg ccgcgcttat caggctctta ccagcaaacg cccctttgtt ggggtgtcgc
    10261 cagtacgtgt tcacgctggg cggaaaaggc aggatcagct tcatactttc aggtccctct
    10321 catgtaacca gtgggttgca cgcagccttg cgttttcctc accggcaagc agtgcgcgga
    10381 taatcccgac cgcctcgctg tcgtcgtcct tcaccgcggt atgaagcgtt atcccccggg
    10441 ccacgccacg ctttatcgtg atgacgcctt ttttctccag tgcgcgaaga tgctccaccg
    10501 ctgcattcac cgaacggtat cccagcatgg ttgccacctc ctgattggtt ggcgggaagc
    10561 cacgttcttt ctgataagaa atcagcatat ccagcacctg ctgctggcat tgagttaacg
    10621 tcgtcatgcc gccatctccc tgaccagttt ttccgcctgc tggcgaacct gcgccagaaa
    10681 cgcctcacca catgcctcaa gttcatcgcg cccgatgtag ctgattgccg gtcccttcca
    10741 ggtcttgtca aaaacagcaa tagcaccagc gaaaaaagct cctgtcagta cctgcttctc
    10801 gtctttcggg ataaaccaga caggcagttc aaaaccaata cgcccgcgaa taaaagcaat
    10861 atgatctgca tcttccggcc accacacttc gctggtggca gctttgatca ggaaaacata
    10921 gcgcccgccc ttatcacgca tggcactggc atgtttcatg atgtaacgca tgccggtgat
    10981 gtattgcccc tcatgctgac tggcgcggct gtatggggga ttaccaaagg cagcaccttt
    11041 aagctccgca agacgttctg accagtcatg cgccagcgcg ttgtcttccg ccgtgtaata
    11101 cgcggcacat ttggcgttat caccgtcagt gaacagatcc agaacaaacg ggccaaacag
    11161 ggtgttaatt ccccagaaaa tgttgtccgg cgtgcgccac tgatcgccca cttccttcag
    11221 ttcatgggct ggtttgttcc gcagttccac cagcgcctgg caatatttat tactcattaa
    11281 gcccccacgt aattccctga cagataccac tcttcacccg atgcagcgcg cttgctgctt
    11341 ttccgtaagc accgctcacg acgcgccaga aaattgtttc gttctggctg ggagtggctt
    11401 tcacggaatg ccgccatcca cacggttgca gcacgacggt ataagcccct ggactccagt
    11461 tcttccgcct ggcgggtcag gcacaaaatc acacggggat cgttagtgcc gacatagaaa
    11521 ttgcgcacag gtctggtttc acgaactggt tgtggttccg gttcctgcgc tctctcagtc
    11581 aggcgcggga aatgtctgcg tgtatctcct tcacaacggt gagccacacg cccactctga
    11641 cgtaacttgc ttgctgactg cagaacgcgc tgccgtgagt aaccggcaaa agcatccgca
    11701 atatctccgg aagtacagcc ccgatgggct tcaatgaatt tctgaacgtc attcaaaaga
    11761 ctcatgctca ccccctgaat cctgccggga tttggctgta gtccacgttg tcgtaactag
    11821 ctttgaagta cgggtcttcg cgtttttcgg tgtacgtgct gacggacggc gataagcgca
    11881 gggaaagctc atcccatttt tcccgcagct tcgacgggct gagcacgtta cggcaccaga
    11941 acggatcgcg actgacgcgg ctgtacatct cgcagatttg tttatgagta cgaccatcct
    12001 gcacacacat caggcgaatt tcgtttgccc aggctgtcca gttcggttct ttgggacgaa
    12061 ccacctcgcc gtcacattcg gcggcctgct cgtacagggc gatgattttt ttccagagcc
    12121 actgtgcgca ggtcaaatca tcctgcgttc cccactggcg ctttttaggg ctgaatacaa
    12181 ccgcatcagg atggcgagtt aaaaactcct gttcagccgt ctgcgtgtcc ggttgcgaag
    12241 cgtccggacg agaagttttt ttatctgacg gatcatgttt tgattttact gacggatccc
    12301 cgccagattc tgacgggtga aaacccgctt ttttgccaga tttcgacgca tcaaattttg
    12361 acgggtcaga ttttgatgcg tcagattttg acgggtcaga atctgacagt tgagaaaatg
    12421 ccgctgcctg aagcttcgca acgttaagct gataaacatt cgacgcattg cggttaccct
    12481 ggcgacgcgc cttacgcgtt aaccagcctt ctgcttccag ccgtgcgata gccgttctga
    12541 cggtactcat tcccgcgcca atctggcggg caatggtttc aattgatggc cagcacacac
    12601 cttcgtcatt actgaaatca gccaggcggg ccataattgc cacgctggat aatttcatgc
    12661 ctgatgcagc gcaaccatcc catacatagc cggttaattt agtgctcatg accgacctct
    12721 atttccctga atttacgacg aaactgttcg agcgggctga agcactcatg ctcatagcct
    12781 tcgcggaggt agataacacg ttgtgtttcc ggctcccaac gaatgactct gacgggcact
    12841 ccgtagtgct ctttgaacca gcggttaact tgtcgcaaag gactgtctcc ttctgccggt
    12901 tgaaatcacc cacagcccac tctgcaaagc tgtgggttac aatttccctg tcacctggta
    12961 catttactgc atagcaatac tccaccttcg cttttccacc cggtacagga agcgcaatca
    13021 gttgcgagcg acggtagtgt gttgttaaac tgttcatgcg ttagtttctc cacagtcacg
    13081 acatgccacg gcgcccggag ctgcacactc gcgggcgtca ctactttctg aaacgcaaaa
    13141 gattttgtag accagtgctg catgctcctg cagcttcgaa attgagaggt actgctcatc
    13201 gttaattgct gtcttctcat gcggttccac tacaccgtct tcaattgctg aacgaatctg
    13261 ttttgaataa ctgccgatct gttcaatgac ttccagcagg cgttggttga tatcggcgtt
    13321 gtccacatcc tcgacgtcag gaagagacac aaagacgcca tttgcagact gcgccacagc
    13381 atcagcaatg aagtgagtgc caccagcacg ctgtaaaacc attgcccatc ccagcgggaa
    13441 aatctgatcg ccatctgcac gaaggcggtt gaataaagcg ttttctgtta catcgagcca
    13501 gtcagccgct tcagcgtaac cacccggcaa cgccgcgata gtttttctga cagctttcac
    13561 gtaccactca ggctgttttt ctattttcca gtgatgctta cccacggtta gcctcatcgt
    13621 tctgtggtta aaaattgaaa gtgttctgct aatctttcgg atagatatcc ggtcttaagt
    13681 cagatttcgt aattgcacct gacgtgcatt gctcaagttt tttagccagc acaaaactgg
    13741 cttttttata gccattgaaa accagccgta agtagccagg tgttgagcca acttttccgg
    13801 ccaactcgcc ctgctgttct ttggttaaag agtcccaata cgctttcata caatatgtac
    13861 ctccggtgta catgttacat gattgaaatg aacctccaag acacttgtac cttaacggta
    13921 caagggtttt aatttcgtta tgaaaacaat ccatgacatc cggcggtcta acgccagaaa
    13981 actgagagat ggtgttggcg ggaattcttc ctttgccact atgattgatc gcgagccaac
    14041 ccagaccagc aggtttatgg gagatggtgc tactaaaaat atcggtgaca gcatggcacg
    14101 acacatcgaa aaatgtttcg acctgcctgt cggatggctc gatcaagaac accagacaac
    14161 gaacatcaca aaaaaacctg atgtttcaat cactaataaa caaatcacat tagtccctgt
    14221 catatcatgg gtacaggccg gagcatggaa agaagttgga tattctgagg ttgatttgag
    14281 cacagcagaa acgtatccct gccctgtacc ctgtggggaa atgacttata tcttgcgggt
    14341 gataggtgat tcaatgattg atgagtaccg cccgggagac atgatttttg tcgatcctga
    14401 agtacctgcc tgccacggtg acgacgttat tgcattgatg cacgatacag gcgaaaccac
    14461 cttcaaaagg ttgatagaag atgggacaca gcgttatctc aaagcgttaa acccaaactg
    14521 gcctgagcct tacattaaga tcaacggtaa ttgctctata attggtacag tgattttctc
    14581 aggaaaacca agaagataca aaatcaaagc ctaatcaatg tttatgaacc tgcttcggca
    14641 ggttttttta tacttgacaa tgtacccttg ggatacataa tgtacccaag cgaaacaacg
    14701 aacaggcagg acgcccacga agtagccgcc tggggcatat gaagtccagg atgattcgtt
    14761 gagtcatgtt gtgccactag gcactcatgt taaagcaggt gtatgaaatg aaagtccaga
    14821 ttttaaacaa taactgtgaa gtcgtttggt catacgacat agccgcccct gtagatcaga
    14881 gcggcgatag ctggaccaat gggaaacatc agattatggc tggagttgtg ttctctttac
    14941 gccgtgcttt ggaacaggct gaagtatttc catcagaccc tgaatggaaa tggccttttt
    15001 ctatttgtcc aaattcggag agcacatttc agaaaattgg tcagaaagtc gcactcgaag
    15061 agcatcagcc aactgtttcc tgattttttc aggtaactcg tcggcatcgc agaaacaaca
    15121 acgctcgatc atgttgaaag ccgattcgta gaactgtttc tgctgagtgt cgctgagaca
    15181 ggaaaagagc gacgttacga tgattttatt aattgcatta tcaagttctt tttcatcaaa
    15241 agtcatttga ttttcctttt atgtatacgg gcttaaaagg ataccaccga gcctgaagtg
    15301 gtgaaaagac aggcacataa cagctaagta ttttcaacca aagagaatcc ttagagttgt
    15361 ggtgaatgcg gctcagcgca cgcgggttaa ggttgaggct gacagtcgac cttctgtgga
    15421 tacccacccg tctggtgtgc aaccttcgcc aggcaccggg aggcacccgg caccacaact
    15481 ttatgctgtg tgtagtcctg gcggtaccag tttgtaccct tgcttccggc tggtaccgtc
    15541 ctttttacaa aacagagaag agcatcaccg gacgacgggc tcataaccca atccatccgg
    15601 gcggctgcta ccgcaggtgt tcttctctgt tttgtggaga aactaatcgg ccttgcaggg
    15661 tcgatatgat gaggagcagc aaaatggcta gcgaacgcag tactgatgtg caggcattta
    15721 tcggggagct ggacggcggc gtatttgaaa ccaaaatcgg cgcagttctc agtgaagtcg
    15781 cttccggtgt gatgaacacg aaaaccaaag gtaaggtctc actcaacctg gaaatcgaac
    15841 catttgatga gaaccgtgtg aaaatcaaac acaaactctc atatgttcgc ccgactaacc
    15901 gcgggaaaat ttccgaagaa gacaccaccg aaacgccgat gtatgtcaat cgcggtggtc
    15961 gcctgactat tctgcaggaa gaccagggac aattactgac tcttgccggt gaacctgacg
    16021 gaaaactccg cgcagcaggt cattaatatc gttcttaatt aactgattat ttatctcatc
    16081 actgaatatc tttatatagt taggacttat tatgtctcag aacttagacg caaccgcaat
    16141 taatcaaatc catgccctta tttctgctca gggtgttaat gaaattatca gtaagattgg
    16201 tgccgatgct gtggcattgc ctgagaattt ccgcattcat gatctggaaa aatttaattt
    16261 aaatcgcttc cgtttccgtg gtgcgctttc cactgccagc atcgatgact ttacccgtta
    16321 ttctaaagat cttgcagatg aaggcacccg ctgctttatc gatgctgata atatgcgtgc
    16381 cgtcagtgtg cttaacctgg gtactattga tgaaccaggt cacgcagata acaccgccac
    16441 tctcaaactg aaaaagacag caccgttctc tgctctgttg tctgttaatg gcgagcgtaa
    16501 ctcccagaaa tcactggcag aatggattga agactgggcc gactaccttg tgggctttga
    16561 tgctaatggt gacaccattc aggcaacaaa agcggctgcg gcggtccgta aaatcacgat
    16621 tgaagcaaac cagaccgctg attttgaaga taatgacttc agcggcaaac gctccctgat
    16681 ggagtctgtc gaagcgaaga ccaaagacat tatgccagtg gcatttgaat ttaaatgcat
    16741 tccgtttgaa ggtctgaaag aacgtccgtt taaattacgc ctcagcatta tcactggcga
    16801 tcgtccggta ctggttctgc gcattattca gctggaggcg gtgcaggaag aaatggctaa
    16861 cgaatttcgt gatctgcttg ttgagaaatt caaggacagc aaagtagaaa cctttattgg
    16921 tactttcacc gcctgatttc attactgcaa atgcccctgc gggggcattt atggaaacgt
    16981 aatttactca ataatcgccg gatggtgagg gattcttttt accagaattc agcgcggtgc
    17041 agcgcatata cgtggagaac aaaatgtcat ttattaaaac tttttccggg aagcattttt
    17101 attatgacag gataaataaa gacgacatcg atattaacga tatcgcggtt tccctttcaa
    17161 atatctgtcg ctttgccggt catctttcgc acttctacag cgtcgcccaa catgcggttc
    17221 tttgcagcca gctggtgccg caggaatttg cttttgaagc gttaatgcat gatgcaacag
    17281 aagcgtattg ccaggacatt cccgcaccac tgaaacgcct tcttcctgac tataaacaaa
    17341 tggaagaaaa aatagactcc gtaatccgtg agaaatacgg attaccccc
//
LOCUS       DAJVQH010000078        17254 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJVQH010000078 DAJVQH010000000
VERSION     DAJVQH010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.078"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(768..1964)
                     /gene="hemY"
                     /locus_tag="OQM87_002678"
     CDS             complement(768..1964)
                     /gene="hemY"
                     /locus_tag="OQM87_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCS1508758.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1967..3172)
                     /gene="hemX"
                     /locus_tag="OQM87_002679"
     CDS             complement(1967..3172)
                     /gene="hemX"
                     /locus_tag="OQM87_002679"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1508759.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3194..3934)
                     /gene="hemD"
                     /locus_tag="OQM87_002680"
     CDS             complement(3194..3934)
                     /gene="hemD"
                     /locus_tag="OQM87_002680"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCS1508760.1"
                     /translation="MSILVTRPSPAGEELVRRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3931..4872)
                     /gene="hemC"
                     /locus_tag="OQM87_002681"
     CDS             complement(3931..4872)
                     /gene="hemC"
                     /locus_tag="OQM87_002681"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCS1508761.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5259..7805
                     /gene="cyaA"
                     /locus_tag="OQM87_002682"
     CDS             5259..7805
                     /gene="cyaA"
                     /locus_tag="OQM87_002682"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCS1508762.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7845..8165)
                     /gene="cyaY"
                     /locus_tag="OQM87_002683"
     CDS             complement(7845..8165)
                     /gene="cyaY"
                     /locus_tag="OQM87_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCS1508763.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8213..9526)
                     /locus_tag="OQM87_002684"
     CDS             complement(8213..9526)
                     /locus_tag="OQM87_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCS1508764.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9523..11001)
                     /locus_tag="OQM87_002685"
                     /pseudo
     CDS             complement(9523..11001)
                     /locus_tag="OQM87_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            11190..11393
                     /locus_tag="OQM87_002686"
     CDS             11190..11393
                     /locus_tag="OQM87_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1508765.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11430..12254
                     /gene="dapF"
                     /locus_tag="OQM87_002687"
     CDS             11430..12254
                     /gene="dapF"
                     /locus_tag="OQM87_002687"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCS1508766.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12251..12958
                     /locus_tag="OQM87_002688"
     CDS             12251..12958
                     /locus_tag="OQM87_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCS1508767.1"
                     /translation="MKQLGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12955..13851
                     /gene="xerC"
                     /locus_tag="OQM87_002689"
     CDS             12955..13851
                     /gene="xerC"
                     /locus_tag="OQM87_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCS1508768.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13851..14567
                     /gene="yigB"
                     /locus_tag="OQM87_002690"
     CDS             13851..14567
                     /gene="yigB"
                     /locus_tag="OQM87_002690"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCS1508769.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14651..16813
                     /gene="uvrD"
                     /locus_tag="OQM87_002691"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14651..16813
                     /gene="uvrD"
                     /locus_tag="OQM87_002691"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCS1508770.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16960..>17254)
                     /locus_tag="OQM87_002692"
     CDS             complement(16960..>17254)
                     /locus_tag="OQM87_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508771.1"
                     /translation="SGAMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQA
                     TNFYDFACYAKAKLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         4013 a         4440 c         4579 g         4222 t
ORIGIN      
        1 gttgacgggg tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca
       61 agacgtttgg gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat
      121 gatgacgtaa ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa
      181 taatagtgac aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag
      241 cgttttcaat cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg
      301 attattgatg ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa
      361 ttttatcatt cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa
      421 gttattactc aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg
      481 ttatttcccg attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt
      541 cgccttagtg cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca
      601 ctgacagatg tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc
      661 acttgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt
      721 atgcatcaga aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg
      781 ttattctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg
      841 tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc
      901 agcgccgcgc ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga
      961 cccaatgtgc tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact
     1021 ttttccagct gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg
     1081 tcatactggc gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat
     1141 tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt
     1201 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
     1261 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
     1321 ttcgccattg atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc
     1381 tgttccgcca gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatctacg
     1441 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
     1501 accggaatgg tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt
     1561 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
     1621 tcagcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc
     1681 agtttcagca gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac
     1741 catccacggg tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac
     1801 agcactacca tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg
     1861 tagttgtcgg tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact
     1921 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
     1981 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg
     2041 caggtttagc ttccgttgtc cccgctgccg gttgtgccag caggttacgc acgcgagtct
     2101 gcatcagttt ttccagcatc gcctggcttt gcagggtttc cggaagatcc atcgagatat
     2161 tttgctggct taactggtcc acctcgtcga ggaacgcttt ggtggtggca tcatcagtat
     2221 cgtagtaagc acgtacccag gtggagacgt tctccagcgc ctggcgataa gtctcttcct
     2281 gatgacgcgg tacagcttgt gctgcgacca gcaggcgaga gcgaatattt tcgcgcagat
     2341 agatatcctg atttggcgct aacagcggta cggcggtgtc atcacgacgg cgaatcgtaa
     2401 tgaagttgtc cataaagttc tgccagcttt tttgcagatt gatacgccat tcgctgatgg
     2461 aactggaaag ctcttcaccg tctgaatcca tcggcgaacc atcgctgtca ttatcggcca
     2521 gacgcaggtt atctacctga tttgaaagct gattaagctt aaggatgatg ccgtcataat
     2581 ccacctgcga tactgcagaa aggctggcga tatcatcggt aattgcccga cgaacggtaa
     2641 tcagactcgg gtcattcata tccgccaggc tggcgtctgc acttttcagc aacgctgcag
     2701 aggtcgtgac gtcctgatcg ctccacagct tccgtccggc gagtttcacc agaaaatcgg
     2761 cctgagccag cagccaggtt ttagcatcgc tgccggaaat ggtggcgatc ttttgttgga
     2821 cttcatccaa ctgttttgcc agcgtttctt gctgacgatt cgcctgctta agctgcgcag
     2881 cttgttgctt aataatgcct tccagctcgg ctttttggct ctcctgggct ttttgcaatg
     2941 ccgtcagttg gttagccagg gcatcgctgg tggcggtctg attgacggcc tgttgtttac
     3001 cccagccata caaaccgaca cccgccgcca gagcaatagc gatagccacc gcgctgagaa
     3061 tcaatgcagt attgttctta ctcttttttt ctgttgcgac aggttgtgac gtggtgtcca
     3121 cggcctccct ggtctcttca accacggcgg aggttttttc ttgttccgtc attatggctt
     3181 cctgttatga gagttattgt aatgcccgta aaagcgcatc gttgtcagcg ttatcggcga
     3241 ccttaatgtc ttgccagccc agttcccggg cgagtttcgc caaacgctca ctgacgacca
     3301 atagtcgaca gtgtagtaac cagtgctcac gataccattg tgggatcagc gaccagagtt
     3361 gctgcaacat ttcaccgctg gtaacaacga ccatcgtcac ctcgcgggat tgccagcgca
     3421 tcgcttcttc tgcaccatcg taatggattg cgcatcgttg ataacattca caaaaagtga
     3481 cctcagcacc gcgcaccgtc agggtatccc caattagctc acgaccgcca ttgccacgta
     3541 atatcagcgc acgtttgccc gcaatatttt gtaattcagg taattgtagc aagacttcgc
     3601 tgatttcccg atcctgtggg tagagaatct tctgtccgct tacggtatgt agcgccagtg
     3661 cggtggtgcg tccaatggtg aaataagtag gtagttgggg ccatttacga tcttgctgat
     3721 gcagctgtga ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac agatcgctct
     3781 cccccagcgc tgccagttga tcagcaagtt gcggtaattg tcgacccgga gaaaactcaa
     3841 tcagcggaaa atgccaggcc acctgcccca gtgtgcgcag acgtctcact aactcttctc
     3901 cagcgggaga cgggcgggtg accaggatac tcatgccggg gcctctccgt tatagacttc
     3961 agcgaggatc tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa tccccatttg
     4021 ttcggcatct tgcggcgcac cgcggcgttc accgcgaata atctgcgaac cgtccggcgc
     4081 gccgaccagc gcacatagcc agatttcgcc atcaataagc tcggcgtagc taccaattgg
     4141 cacctgacat ccgccttcga gacgggtatt catggcgcgt tctgcggtaa cgcgcagtgc
     4201 agtttcgtgg tgattcagcg cggcaagcag ctcgcgagtg cgtgtatcat caaggcggca
     4261 ttcaataccg actgcacctt gtcctaccgc cggaagagaa atctcgggtg gtaacgcggc
     4321 gcgaatgcgg gactccagac ctaaacgttt tagtccggct acggcaagaa tgatggcatc
     4381 gtattcgccg ttatctagtt tgctcaggcg agtgccgacg ttgccgcgca gggagcggat
     4441 aatcagatcc gggcggcgtt cagccagttg gcactggcga cgtaaactgg acgtcccgac
     4501 gatactgcct gctggtaacg catccagact gtcatagtta ttggacacaa aggcatcgcg
     4561 aggatcttca cgctcgcaaa tagtgaccag ccccagacct tgcgggaatt caaccggcac
     4621 atctttcatt gaatgtacgg cgatatcagc gcgattctcc aggagcgcga cttccagctc
     4681 ttttacaaat aagccttttc cgcctacttt cgccagcggc gtatcaagaa tcacatcgcc
     4741 gcgcgtcacc atcggtacca gttcaacgac caggcccgga tggctcgcca tcaacttgtc
     4801 tttgacatag tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa ttcttaaaac
     4861 attgtctaac atgcttgtta ccgtcattat catccgtggt ccatcctaac atccttgcca
     4921 gagtgatgtc agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa atgaggtctg
     4981 gcagtggatc ctgacaggcg tttcacgccg tcgtaataag gaatttacag agaataaacg
     5041 gtgctacact tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt gttaaattga
     5101 tcacgtttta gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga aaagtggtaa
     5161 cggttacctt tgacatacga aatatcccga atgccgcgtg ttaccgttga tgttggcgga
     5221 atcacagtca tgacgggtag caaatcaggc gatacgtctt gtacctctat attgagactc
     5281 tgaaacagag actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt gctgctatgg
     5341 ggcctgcatt ccaacaggtc tacagtctac tgccgacatt gttgcactat caccatccgc
     5401 taatgccggg ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac acgcctgatg
     5461 aaactcaacg ccactacctg aacgagcttg aactgtatcg tggaatgtca gtacaggatc
     5521 caccgaaagg tgagcttcca attactggtg tatacaccat gggcagcacc tcgtccgtag
     5581 ggcaaagttg ttcctctgac ctggatatct gggtctgtca tcaatcctgg ctcgatagcg
     5641 aagagcgcca gttactacaa cgtaaatgca gcctgctgga aagctgggcc gcctcgctgg
     5701 gtgtggaagt cagcttcttc ctgattgatg aaaaccgctt ccgtcataat gaaagcggca
     5761 gcctgggggg cgaagattgt ggctccaccc agcatatact gctgcttgac gaattttatc
     5821 gtactgccgt gcgtctcgcc ggtaagcgta ttctgtggaa tatggtgccg tgcgacgaag
     5881 aagagcatta cgacgactat gtgatgacgc tttacgcgca gggcgtgctg acgccaaatg
     5941 aatggctgga tctcggtggc ttaagctcgc tttctgctga agagtacttt ggtgccagcc
     6001 tttggcagct ctacaagagt atcgattccc catacaaagc ggtgctgaaa acactgctgc
     6061 tggaagccta ttcctgggaa tacccgaacc cacgtctgct ggcgaaagat atcaaacagc
     6121 gtttgcacga cggcgagatt gtatcgtttg gcctcgatcc atactgcatg atgctggagc
     6181 gtgttactga atacctgacg gcgattgaag atttcacccg tctggattta gtacgtcgct
     6241 gcttctattt aaaagtgtgc gaaaaactca gccgtgaacg cgcctgtgta ggctggcgtc
     6301 gcgcagtgtt gagccagtta gtgagcgagt ggggttggga cgaagctcgt ctggcaatgc
     6361 tcgataaccg tgctaactgg aagattgatc aggtgcgtga ggcgcacaac gagctgctcg
     6421 acgcgatgat gcagagctac cgtaatctga tccgctttgc gcgtcgcaat aaccttagcg
     6481 tctccgccag tccgcaggat atcggcgtac tgacgcgtaa actgtatgcc gcgtttgaag
     6541 cattgccagg taaagtgacg ctggtaaacc cgcagatttc acccgatctc tcggaaccga
     6601 atctgacctt tatttatgtg ccgccgggcc gggctaaccg ttcaggttgg tatctgtata
     6661 accgcgcgcc aaatattgag tcgatcatca gccatcagcc gctggaatat aaccgttacc
     6721 tgaataaact ggtggcgtgg gcatggttta acggcctgct gacctcgcgc acccgtttgt
     6781 atattaaagg taacggcatt gtcgatttgc ctaagttgca ggagatggtc gccgacgtgt
     6841 cgcaccattt cccgctgcgc ttacctgcac cgacaccgaa ggcgctctac agcccgtgtg
     6901 agattcgcca tctggcgatt atcgttaacc tggaatatga cccgacagct gcgttccgca
     6961 atcaggtggt gcattttgat ttccgtaagc tggatgtctt cagctttggc gagaatcaaa
     7021 attgcctggt aggtagcgtt gacctgctgt accgcaactc gtggaacgaa gtgcgtacgc
     7081 tgcacttcaa cggcgagcaa tcgatgatcg aagccctgaa aactattctc ggcaaaatgc
     7141 atcaggacgc cgcaccgcca gatagcgtgg aagtcttctg ttatagccag catctgcgcg
     7201 gcttaatccg tactcgcgtg cagcaactgg tttctgagtg tattgaactg cgcctttcca
     7261 gcacccgcca ggaaaccggg cgtttcaagg cgctgcgcgt ttctggtcaa acctggggct
     7321 tgttcttcga acgcctgaat gtatcggtac agaagctgga gaacgccatc gagttttatg
     7381 gcgctatttc gcataacaaa ctgcacggct tgtcagtgca ggttgaaacc aatcacgtca
     7441 aattaccggc ggtggtggac ggctttgcca gcgaagggat catccagttc tttttcgaag
     7501 aaacgcaaga cgagaatggc tttaatatct acattctcga cgaaagcaac cgggttgagg
     7561 tgtatcacca ctgcgaaggc agcaaagagg agctggtacg tgacgtcagt cgcttctact
     7621 cgtcatcgca cgaccgcttc acctacggct caagcttcat caacttcaac ctgccgcagt
     7681 tctatcagat tgtgaaggtt gatggtcgcg aacaggtgat tccgttccga acaaaatcga
     7741 ttggcaacct gccgcccgcc aatcaggatc acgatacgcc gctattacag cagtattttt
     7801 cgtgatgagc gtgccggaaa gcgaggctta tccggcatgc aatcttagcg gaaactgact
     7861 gtttcacccg cctgttgcgt cgccgcctgt tccagcaaat cccagaaggt ttcgccgctg
     7921 cgatcgcaaa tccattcatc gcctttcagg tcaaaatggt agccgccctg tttggttgcc
     7981 agccacacct ggtgcagcgg ctcctggcgg ttaataatga ttttgctgcc gttttcaaag
     8041 gtaatggtca gtacgccgcc gttgatttcg cagtcgatat cgctgtcgcc atccctgtcg
     8101 tccagacgct cttcaatggt cagccacagt tgatcagcca ggcgatgaaa ttcactgtcg
     8161 ttcattgttg taccctgttt ttaagtgatg gcggcagtat agcggcatgg ggtcagggct
     8221 tcaaagtttg cacctctgct gctgcgttcc ggcatgattc atccgtcacc ggaataatga
     8281 tgtctctgtt ttgaacttca cgtacgttct cctctctgac acgagttttc gccgggcagg
     8341 gggagggcgt aatccttacc tctgtatcgg tccatgtata aacattgcga tcgtcataag
     8401 cgaagcgatc gtttttagtt agcggaccgt taactggaat aatttgcaag ctctttgacc
     8461 gtaattggcg gataaagtaa gcatgctgtt tgccgctatt aaatgagcga tcaatgacca
     8521 agtctggcgt atccagcggg attatctcaa gctggtcttc accaaatgtc gataattttg
     8581 ttacatacag ccgatcttta tattgcgcaa tattgccagt aatcagatgg aattgttcag
     8641 gttcaatacc tggtaggtct atccacttgc tccactgacg gtcgggcgtg agctgtttac
     8701 gccagcgaac gcgactttcc cctacttcaa aagagacgtc atcatctacg ggtatcgctt
     8761 tgcctgataa ttgaccaaag ggataacgat gcagaccctt attgtcgcga aaaaccagca
     8821 gtgaatgagg tatccagcga attatctcaa atgagtctgc attcgccgta aggtgaacgc
     8881 catttaaata aatattctgc ccactgcgga taagcagatc attggaatat gaatttctgt
     8941 ttacactcat aagacgtgga ttaatacccc accatctttt ttctaataca gtaacatcac
     9001 tgctgctccc ctgacgtcga ccggacaagt agagattttt actatccacc agcgtggtag
     9061 agttaccttc gatagctttt agtgtcgcca gatcaacacg tgacgcgccg ctattacttt
     9121 cggtgcgctg tccgtcaaaa tagaggctat atttatcgac cgcgaagcgg cccatagcct
     9181 gaaagctggc aatatcgaca gtgggtgttc ccggtggatt gctaacgatt ttgccacgcc
     9241 agagaataac gcggccgtct gtcacccagt ggtattcctc attttctatg cttatttcat
     9301 cggaaggtat ataacccgtt ttcccagaga tatttttcac aaggcgcaaa ccggccctgc
     9361 tcatctccgg aaactgggca atgattttct tatcccccca ataaccttta ccgtaagaca
     9421 taacaccgtg atcattgtaa tagtcgaacc ctatggcctg tacagcaggc gtcagaaatg
     9481 tgtgtcccaa caggatgagt gcaactgtga ttatttgacc cttcattttg tcacctcatt
     9541 gcgcggggca ggaataaatt cgccgtcatc agtaattttt ccgtccagat atcccgtttt
     9601 gtaatttacg ccatagacat atcccgaatg ttgttccttc cagattaagc gaacttgttt
     9661 cggatcagcg gtgcggtatc gccagagctg cgcgccatca aagctgtaaa tgtagcggtt
     9721 atcgcgggca taccatctgc gcaaaaaggg atggtcatta agatgggctt cgcgttcatc
     9781 cggagaacgg ttatccagta agatcagcgg tccgtcagat tcaaacacgt gtaaaccgga
     9841 atggtatggc tacccttcgg cacgcgtaag taaatagccg tggcgtttcc cggcgttgaa
     9901 acgtttgttg ataataagat tgggatcatc aagtttcacg atctccagct tacggtcacc
     9961 agactctgtt tcgatgatgg tataaagcga gtcctgatac tgggcgacag catcactgat
    10021 gggttgaaac tgctccggat cgagtccggg taattcttcc tgtttacaag caggtccttt
    10081 acgccagaac actttctttt ccagcagaga aaaatccaga cagtgctttg ttaaatcttc
    10141 atcccacgcc agattttcct tgtcgaggac tttacgctgc agcccgtttt tatcacgcca
    10201 ggtcaagagt gagccaggca tccagcgcac gacggaaaag gtgtccgggt cggcatcaag
    10261 ctgttcgccg ttgagcagga ttttggtccg tgtacgagcc agggtatccc aggggccaaa
    10321 tggtacggca atgcaggggt taaacgtggt ggaaaacttt cctcgctgat cccaggactt
    10381 ctgcgccagc acacggaaac tctcggggtc agcgaggcga tgcccattga tatacaagaa
    10441 attagcgtca cgtaatatca gccccagtaa gtcaggatcc cagggaggcc ggaattccac
    10501 ctgatgcagc gttttgatat ccaagctatt tccaccacca ttgtcatcgg tgcgcttgcc
    10561 ttcaaaatag agactgtgtt tatctgcggc aaaatcaccc catgccttga acgaggcgac
    10621 atcgacggga ggtgtgccgg gagggttttg cacaacttca cccgcgtaga ggatattgcg
    10681 accatcgcta tgccatgaat aatgaggata ctaggcctgt gcgccctcat agaggtagtc
    10741 ggttatcaaa tcagtcagag gcggttcttt atcccattcg tctagtgttg tcgggtctat
    10801 taataaattg ggtaaccgac gcaaactctt gtaattcacg tcaggtagag aaagatttgt
    10861 ttctttataa gggcgcaggt acaccacgcc ttttccctgt ttctggtaaa tattatgttc
    10921 aggtgcggaa agccgacatc cccatgccga cgataaaaac aatgaacaag ccaacaaaca
    10981 aaccacattg cgatagtgca taaagccatc ctggcgcgag gtgttgatca cgaaactgcc
    11041 agcagaacat aaatcctcac gagtaagcgt tatactcgca gcatttcctc acttttcaga
    11101 cttcataaag agtcgctaaa cgcttgcttt tacgccttct cctgcgatga tagaaagcag
    11161 aaagcgatga actttacagg caatccataa tgaaaaacgt gtttaaggca ctcactgtat
    11221 tacttactct cttcagcctg acgggctgcg gtctgaaagg tccgctctat ttcccgcctg
    11281 cagataaaaa cgcaccgccg ccgaccaaac cggtagagac gcaaacgcaa tccacggtgc
    11341 cggataaaaa cgatcgcgcc actggcgatg gtccatccca ggtgaattac taaaagtcag
    11401 tttctgtacc cgcgtgattg gagtaaatga tgcagttctc gaaaatgcat ggccttggca
    11461 acgattttat ggtcgtcgac gcggtaacgc agaatgtctt tttttcaccg gagctgattc
    11521 gtcgcctggc ggatcggcac ctgggggtag ggtttgacca gctgctggtg gttgagccgc
    11581 cgtatgatcc tgaactggat tttcactatc gcattttcaa tgctgatggc agtgaagtgg
    11641 cgcagtgcgg taacggtgcg cgctgctttg cccgttttgt gcgtctgaaa ggactgacca
    11701 ataagcgtga tatccgcgtc agcaccgcca acgggcggat ggttctgacc gtcaccgatg
    11761 atgatctggt ccgcgtaaat atgggcgaac ccaacttcga accttccgcc gtgccgtttc
    11821 gcgctaacaa agcggaaaag acctatatta tgcgcgccgc cgagcagaca atcttatgcg
    11881 gcgtggtgtc gatgggaaat ccgcattgcg tgattcaggt cgatgatgtc gataccgcgg
    11941 cggtagaaac gcttggtcct gttctggaaa gtcacgagcg ttttccggag cgcgccaata
    12001 tcggttttat gcaagtggtt aagcgcgagc atattcgttt acgcgtttat gagcgtgggg
    12061 caggagaaac ccaggcctgc ggcagcggcg cgtgtgcggc ggtggcggta gggattcagc
    12121 aaggtttgct ggacgaagaa gtacgcgtgg aactccccgg cggtcgtctt gatatcgcct
    12181 ggaaaggtcc gggtcacccg ttatatatga ctggcccggc ggtacatgtc tacgacggat
    12241 ttattcatct atgaagcaac taggggaaga actgcaggaa acactcacgg agcttgatga
    12301 ccgggcggtt gtcgattatc tgattaaaaa tcctgagttt tttatccgca atgcgcgcgc
    12361 agtagaagcg atacgtgtgc cgcatccggt acgcggcacc gtttcgttgg tcgagtggca
    12421 catggcccgc gcacgtaatc acattcatgt tctggaagag aacatggcgt tgttgatgga
    12481 gcaggctatc gccaacgaag ggctgtttta tcgcctactc tacctgcagc gcagtctcac
    12541 cgccgccagc agtctcgacg atatgctgat gcgctttcac cgctgggcgc gcgatctcgg
    12601 cctggctggt gcgagtctgc gcctgtttcc agatcgctgg cgcttaggtg cgccgtcgaa
    12661 ccacactcat ctggcattaa gccgtcagtc tttcgaaccg ctgcgtattc agcgtttggg
    12721 gcaggaacag cactatcttg ggccgcttaa cggaccggag ctgctggtgg tgctaccgga
    12781 agcgaaagcg gtgggatcgg tggcgatgtc gatgctggga agcgatgctg atttgggtgt
    12841 tgtgctgttt accagtcgcg atgccagtca ctatcaacaa gggcagggaa cgcagttact
    12901 tcatgaaatt gcgctgatgt tgccggagct tctggagcgt tggattgaac gcgtatgacc
    12961 gatttacaca ccgatgtaga acgctaccta cgttatctga gcgtggagcg tcagcttagc
    13021 ccgataaccc tacttaacta ccagcgtcag cttgaggcga tcatcaattt tgccagcgaa
    13081 aacggcctgc aaagctggca acaatgcgat gcagcgatgg tacgcaattt tgctgtacgc
    13141 agtcgccgta aagggctggg agcagcaagt ctggcgttac ggctttctgc gctacgtagc
    13201 ttttttgact ggctggtcag ccagaacgaa ctcaaagcta acccggcgaa aggagtttcg
    13261 gcaccgaaag cgccgcgtca tctgccgaaa aatatcgacg tcgacgatat gaatcggctg
    13321 ctggatattg atatcaacga tcccctcgct gtacgcgacc gtgcaatgct ggaagtgatg
    13381 tacggcgcgg gtctgcgtct ttccgagctg gtggggctgg atatcaaaca cctcgacctg
    13441 gagtccggcg aagtgtgggt gatggggaaa ggcagcaaag agcgccgcct gccgattggt
    13501 cgcaacgctg tggcgtggat tgagcactgg cttgatttgc gcgacctgtt tggtagcgaa
    13561 gacgacgcgc tttttctgtc gaaactgggc aagcgtatct ccgcgcgtaa tgtgcagaaa
    13621 cgctttgccg aatggggcat aaaacaaggg ctgaataatc acgttcaccc acataaatta
    13681 cgtcactcgt tcgctacgca tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag
    13741 ctactgggtc atgccaacct ctccaccacg caaatctata ctcatcttga ttttcaacac
    13801 cttgcctcgg tgtacgatgc ggcgcatcca cgcgccaaac gggggaaata atgcgttttt
    13861 accggccttt ggggcgcatc tcggcgctca cctttgacct ggatgatacc ctttacgata
    13921 accgtccggt gattttgcgc accgagcgag aggcgcttac ctttgtgcaa aattatcatc
    13981 cggcgctgcg cagcttccag aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag
    14041 cggaacccga gatttatcac gacgtgacgc gttggcgttt tcgttcgatt gaacaagcga
    14101 tgctcgacgc cgggctgagt gccgaagaag ccagtgcagg cgcacacgca gcaatgatta
    14161 actttgccaa atggcgcagc cgaatcgacg tcccgcagca aactcacgac accttaaaac
    14221 agctggcgaa gaaatggccg ctggtggcga tcaccaacgg taacgcccag ccggaactgt
    14281 ttggtttggg ggattatttt gagtttgtgc tgcgcgctgg cccgcacggg cgctcaaaac
    14341 cgttcagcga tatgtacttt ttggctgcgg aaaaactcaa cgtgccgatc ggcgagatct
    14401 tacatgttgg ggacgatctc accactgacg tgggtggggc aattcgcagc ggaatgcagg
    14461 cttgttggat cagaccggaa aatggcgatc tgatgcaaac ctgggacagc cgtttactgc
    14521 cacatctgga aatttcccgg ttggcatctc tgacctcgct gatataatca gcaaatctgt
    14581 atatataccc agctttttgg cggagggcgt tgcgcttctc cgcccaacct atttttacgc
    14641 ggcggtgcca atggacgttt cttacctgct cgacagcctt aatgacaaac aacgcgaagc
    14701 ggtggccgcg ccacgcagca accttctggt gctggcgggc gcgggcagtg gtaagacgcg
    14761 cgtactggtg catcgtatcg cctggctgat gagcgtggaa aactgctcgc catactcgat
    14821 tatggctgtg acgtttacca acaaagcggc ggcggagatg cgtcatcgta tcggacaact
    14881 gatgggcacc agccagggcg gcatgtgggt aggcaccttc cacgggctgg cgcaccgcct
    14941 gctgcgtgcg caccatatgg acgccaatct gccgcaggat ttccagatcc tcgatagcga
    15001 agaccagcta cgcctgctta agcgtctgat caaagccatg aacctcgacg agaagcagtg
    15061 gccgccgcgc caggcaatgt ggtacatcaa cagccagaaa gatgaaggcc tgcgtccgca
    15121 tcatattcaa agctacggta atccggtgga gcagacctgg cagaaggtgt atcaggcgta
    15181 tcaggaagcg tgcgatcgcg cgggcctggt ggacttcgcc gagctgctgc tgcgcgctca
    15241 cgaactgtgg cttaacaagc cgcatatcct acaacactac cgcgaacgtt ttaccaatat
    15301 cctggtggac gaattccagg ataccaacaa cattcagtac gcgtggatcc gcctgctggc
    15361 gggcgacacc ggcaaagtga tgatcgtcgg tgatgacgac cagtcaatct acggctggcg
    15421 cggggcgcag gtggagaata ttcagcgttt ccttaatgat ttccccggtg ccgaaattat
    15481 tcgtctggag caaaactacc gctctaccag caatattctg agcgccgcta acgccctgat
    15541 tgaaaacaat aacgggcgtc tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc
    15601 tatttccctc tattgcgctt ttaacgaact cgatgaagcg cgttttgtgg ttaaccgcat
    15661 caaaacctgg caggacaacg gcggggcgct tgccgagtgc gccattctct accgcagtaa
    15721 cgcccagtcg cgtgtgctgg aagaagcact gttgcaggcg agtatgccgt accgtattta
    15781 cggcgggatg cgcttcttcg aacgccagga aatcaaagat gcgctctcgt atctgcgcct
    15841 gattgccaac cgtaacgacg acgcggcctt tgagcgtgtg gtgaatacgc caacgcgggg
    15901 tattggtgac cggacgctgg acgtggtacg tcagacatcg cgcgatcgcc agttaacact
    15961 ctggcaggca tgtagtgaac tgttgcagga aaaagccctc gccggacgtg ctgccagcgc
    16021 cttacagcgg tttatggaac tgatcgacgc cttagcgcag gaaactgccg atatgccgct
    16081 gcatgtacag actgaccggg taattaaaga ctccggcctg cgtaccatgt acgagcagga
    16141 gaagggcgaa aaaggtcaga cgcgtatcga aaacttagag gaactggtga cggcaacgcg
    16201 ccagttcagc tacaacgaag aagacgaaga tttaatgccg ctgcaggcgt tcctatccca
    16261 tgcggcgctg gaagcaggcg aagggcaggc ggatacctgg caggatgcgg tacaactgat
    16321 gaccatgcac tcggcgaaag gtctggagtt cccgcaggtg tttatcgtcg gtatggaaga
    16381 gggcatgttc ccaagccaga tgtcgctgga tgaaggcggg cgtctggaag aagaacgccg
    16441 tctggcctac gttggcgtaa cccgcgcgat gcagaaactg acgctgacct acgcggaaac
    16501 tcgccgtctg tatggcaaag aggtttacca tcgcccgtcg cgctttattg gcgagttgcc
    16561 ggaagagtgt gtggaagagg tgcgcctgcg cgccacggta agccgcccgg tcagccatca
    16621 gcggatgggt acgccgatgg tcgagaacga cagcggctac aagctcggcc agcgtgtacg
    16681 ccacgctaag tttggtgaag gcaccatcgt caatatggaa ggcagcggtg aacatagccg
    16741 tttgcaggtg gcattccagg gccagggaat caaatggctg gtggcagctt acgcccggct
    16801 ggagtcggtg taacgttgcc ggatgcggtg ctgcgcacct tatttggcct aaaaaatcat
    16861 tcagattcaa taaattgcaa cgtcatgtag gcaggataag gcgtttacgc cgcatccggc
    16921 atcagcagca tatttagtat ctgacacaaa gctatcattc taacctttcc gctcgacgga
    16981 aatcatggtg acaaaaggat agcgttgcca cgggattgcc ccgcctttca tatacatatg
    17041 tgaaatcgtg ccaccaagat aaagcagctg ctcaacgttc agtttcgctt tggcataaca
    17101 ggcaaaatca taaaaatttg tcgcctgctg gctcaacaaa aacacggcat tcccatgttt
    17161 attaatccca acaccgttac gaattttacg tgaggcgacg ttgggatgaa tacgcggatt
    17221 aattacaccg ttttccatca acattgctcc tgac
//
LOCUS       DAJVQH010000079        17123 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJVQH010000079 DAJVQH010000000
VERSION     DAJVQH010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.079"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..736
                     /locus_tag="OQM87_002693"
     CDS             <1..736
                     /locus_tag="OQM87_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1508772.1"
                     /translation="SQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVT
                     YPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLGS
                     GNPRNFTCNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFY
                     LSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGLS
                     ATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1070..3349
                     /gene="ydeP"
                     /locus_tag="OQM87_002694"
     CDS             1070..3349
                     /gene="ydeP"
                     /locus_tag="OQM87_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCS1508773.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3597..3794
                     /gene="safA"
                     /locus_tag="OQM87_002695"
     CDS             3597..3794
                     /gene="safA"
                     /locus_tag="OQM87_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCS1508774.1"
                     /translation="MHATTVKNKIMQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3868..4629
                     /gene="ydeO"
                     /locus_tag="OQM87_002696"
     CDS             3868..4629
                     /gene="ydeO"
                     /locus_tag="OQM87_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCS1508775.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5031..5438
                     /locus_tag="OQM87_002697"
                     /pseudo
     CDS             5031..5438
                     /locus_tag="OQM87_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(5464..6657)
                     /locus_tag="OQM87_002698"
     CDS             complement(5464..6657)
                     /locus_tag="OQM87_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCS1508776.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            6740..8024
                     /locus_tag="OQM87_002699"
                     /pseudo
     CDS             6740..8024
                     /locus_tag="OQM87_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
     gene            8076..9231
                     /locus_tag="OQM87_002700"
                     /pseudo
     CDS             8076..9231
                     /locus_tag="OQM87_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(9197..9319)
                     /locus_tag="OQM87_002701"
     CDS             complement(9197..9319)
                     /locus_tag="OQM87_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508777.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            9522..11207
                     /locus_tag="OQM87_002702"
                     /pseudo
     CDS             9522..11207
                     /locus_tag="OQM87_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            11245..13617
                     /locus_tag="OQM87_002703"
     CDS             11245..13617
                     /locus_tag="OQM87_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1508778.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNTPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            13674..16457
                     /locus_tag="OQM87_002704"
     CDS             13674..16457
                     /locus_tag="OQM87_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1508779.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETFESMG
                     LRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     SEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFLLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            16819..>17123
                     /locus_tag="OQM87_002705"
     CDS             16819..>17123
                     /locus_tag="OQM87_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1508780.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT         4975 a         3756 c         3719 g         4673 t
ORIGIN      
        1 gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg atcatataaa
       61 cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat cgctgtactg
      121 gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg atattggtga
      181 taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg cggccggtgg
      241 agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa ttgcaacatt
      301 aggaagcgga aatccgcgta attttacgtg taatattatt tcaaataaca gtgttgttat
      361 gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc cggactttcc
      421 tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc tctcatttta
      481 tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag cacctgatgc
      541 aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac tggctacggg
      601 agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt taagcgcaac
      661 ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca ttggtgtcac
      721 ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc acgaaaattg
      781 cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc cgcgcacaat
      841 tgaagccaga gtcttatttt ttatattgaa aaatatgtta atttaaacac tatgagagat
      901 gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa aatagaaaga
      961 gagtagtccc ctactcttat atatccatgt tggcgataat ccctttgtat gtactgtgca
     1021 tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga tgaagaaaaa
     1081 aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg tagcgaatgc
     1141 agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca tgaataagcc
     1201 agagggcttt gactgtccgg gttgtgcatg gccagatcct aagcacagtg cgtcattcga
     1261 catttgtgaa aacggcgcaa aagcaatcgc ctgggaagtc acggataagc aggtaaatgc
     1321 ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc acgagcttga
     1381 ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact gttacaagcc
     1441 attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc
     1501 caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct ttctttatca
     1561 gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca tgtgccatga
     1621 accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg tgttgctgga
     1681 agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta caaaccaccc
     1741 tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa
     1801 tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt ttgaaatgct
     1861 gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg gtggcgatat
     1921 ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc
     1981 aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg gctttgacga
     2041 gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt ctggactaag
     2101 tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca ccattatctg
     2161 ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc aactggtcaa
     2221 tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc cactacgtgg
     2281 tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt
     2341 tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg gacatgctgc
     2401 aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct gcatgggggg
     2461 caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa cgcaattaga
     2521 tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg cacggcatag
     2581 ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg gtgcgcaggc
     2641 ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc
     2701 cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc
     2761 ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc gcaatgacat
     2821 tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc ccggtggttt
     2881 tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt
     2941 cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta agctggtcat
     3001 ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg atgatcgcta
     3061 tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag ctaaaatttg
     3121 ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg gtaagcgcag
     3181 ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc gctcactggt
     3241 gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc cattaagtgg
     3301 cattcctggc tataaaagta ttccggttga attagaacca tcaaattaat gcctcttctc
     3361 atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta ttgcgcaatt
     3421 tactcacatg gattaaatta aactacatac tataagatat aaacttctgc ctacagctgt
     3481 aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc agcctgttat
     3541 attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata tttgaaatgc
     3601 atgcgaccac agtgaaaaac aaaatcatgc aaagagataa ctataaagaa atcatgtctg
     3661 caattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg gcaattgatc
     3721 agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc attatcaata
     3781 gtttgcaaga ctgaaaataa acagcaaatt ataaatgtga aatgtttaaa aggtatcgat
     3841 aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat atttattcat
     3901 catgctttca acgctaacat tttagataaa gattacgcct tctctgacgg cgagatcctg
     3961 atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt taaagaaatc
     4021 ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca gacagtgacg
     4081 gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt gcttaatgaa
     4141 atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga acttttgctt
     4201 ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt aactaacggt
     4261 gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc gcacccatgg
     4321 aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaacta
     4381 aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca gcacgcaaaa
     4441 aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg ttatgccagt
     4501 acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa gagagtttct
     4561 aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat gagtgcttta
     4621 gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga attatatggg
     4681 gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat aaataagtga
     4741 catccatcac atatttatgc acttgcataa cctgttgcat gattatttat aatctcaatt
     4801 cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag ttatttatcg
     4861 gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact gttatttccc
     4921 tttttttagc taaatctgct atctctttat gtgactaact tcacttacat ccacttattt
     4981 ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt atgaagtctg
     5041 cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct ggtatggctg
     5101 catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca aacgttgctt
     5161 tctcagactt tacgccgact gaatacagta ccaaaggaaa gccaaatatt atcgtactaa
     5221 ccatggatga tcttggttat ggacaacttc cttttgataa gggatctttt gacccaaaaa
     5281 caatggaaaa tcgtgaagtt gtcgatacct acaaaatagg gatagataaa gccattgaag
     5341 ctgcacaaaa atcaacgccg acgctccttt cattaatgga tgaaggcgta cgttttacta
     5401 acggctatgt ggcacacggt gtttccggcc cctcccgtct gagggatccc cacaaaatta
     5461 ctgctaataa accagcacca tatttcggta ggttctggac agatggttta gaactgtgct
     5521 cagtactgtt cgtcttaaaa taagtggcga gtgtcgaaga acattcttcg ctaacgtcag
     5581 atatgagata acccgccggg atttaatgct gttagcctga tatttcaggt gtaacccttt
     5641 attttcagcg tgataaccga gtaaccacat tacgatagtg ctcaacgttg ccagcagact
     5701 cagaaccaga attcttcctg ttgaacgact tttgctggcc cgaagaccaa atccgaagcg
     5761 cccgttcttt tcatcacgaa agttttgctc tatctgcatt cgccgactgt ataacttgat
     5821 gatttctcgc gctctgaagt catttgtact gctgaagatc aaccaggctt cttttgctga
     5881 tttgctctgt tctttgtctg ttttgtttaa accactctga cctttcgaac gtttcttttt
     5941 tcgccctttt gccgatttct tataggtgta aaaatgcccc cggatgcttt ttttcctttc
     6001 tttaaccagc cttccagccc ccatatattc cggtgtttta cattcagggc tatccgacac
     6061 ttttaaccat ctttcaggcg cgttatcgag gcaatactga acgttatttc tgatccgacc
     6121 aataaaatcc cagcccagag aagtaatatg gtgaaaccat gcgctctgaa atccagcatc
     6181 cgtgacaata ataaccctgg catcgggggg cagggattga gcaagggaat caaggaaatc
     6241 atgttgtatc aatggattat tttgtttttc agaaggaaca accttactca ataacggaat
     6301 ggaacgcccg tcacacagaa ggctggcacg aagaacatga tgctcctggg agggatagcc
     6361 actccagtct acggctatta cacataaaga taactgccgt gtcagcattg aagtgatatt
     6421 cctgaaaatc attggaatat cgcgatggag cgcctcatta cccatgaggc gatcaatacg
     6481 tttgatctta ttcttaactt gtgcattacc cggcaaaaaa cgtccgatac tggtctgcgt
     6541 cagagatgct ccgttgatta acgccattgt ggcatccatc agggcatttt gtcggtactg
     6601 atgaaatggt gctaaggcat cccggaagaa attctgacat actttatgaa caggcatagc
     6661 agtgatctca ttgaattgtt ggcacaaatc agtagatcac ataatgctat gtttgtctcg
     6721 ttttctgggg attcctcagc cctcccgtgc cgcaataatg accggtcgag ctcccgcccg
     6781 ctttggtgtc tattccaata ccgatgctca ggatggtatt ccgctaacag aaactttctt
     6841 gcctgaatta ttccagaatc atggttatta cactgcagca gtaggtaaat ggcacttgtc
     6901 aaaaatcagt aatgtgccgg taccggaaga taaacaaacg cgtgactatc atgacaactt
     6961 caccacattt tctgcggaag aatggcaacc tcaaaaccgt ggctttgatt actttatggg
     7021 attccacgct gcaggaacgg catattacaa ctccccttca ctgttcaaaa atcgtgaacg
     7081 tgtccccgca aaaggttata tcagcgatca gttaaccgat gaggcaattg gcgttgttga
     7141 tcgtgccaaa acacttgacc agccttttat gctttacctg gcttataatg ctccgcacct
     7201 accaaatgat aatcctgcac cggatcaata tcagaagcaa tttaataccg gtagtcaaac
     7261 agcagataac tactacgctt ccgtttattc tgttgatcag ggtgtaaaac gcattctcga
     7321 acaactgaag aaaaacggac agtatgacaa tacaattatt ctctttacct ccgataatgg
     7381 tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa aaaggctata agagtcagac
     7441 ctatcctggc ggtactcacc accccaatgt ttatgtggtg gaaaggaaaa cttcaacccg
     7501 gtaattatga caagctgatt tccgcaatgg atttctaccc aacagctctt gatgcagccg
     7561 atatcagcat tccaaaagac cttaagctgg atggtgtttc cttgctgccc tggttgcaag
     7621 ataagaaaca aggcgagcca cataaaaatc tgacctggat aacctcttat tctcactggt
     7681 ttgatgagga aaatattcca ttctgggata attaccacaa atttgttcgc catcagtcag
     7741 acgattaccc gcataacccc aacactgagg acttaagcca attctcttat acggtgagaa
     7801 ataacgatta ttcgcttgtc tatacagtag aaaacaatca gttaggtctc tacaaactga
     7861 cggatctaca gcaaaaagat aaccttgccg ccgccaatcc gcaggtcgtt aaagagatgc
     7921 aaggcgtggt aagagagttt atcgacagca gccagccacc gcttagcgag gtaaatcagg
     7981 agaagtttaa caatatcaag aaagcactaa gcgaagcgaa ataactaaac cttcatgcgg
     8041 cggatttttc cgccgcctta ttgagcgaga tagcaatgca cgttacagcc aagccctcca
     8101 gttttcaatg taatctcaaa tgtgattact gtttttacct tgaaaaagag tcgcagttta
     8161 ctcatgaaaa atggatggat gacagcactc tgaaagagtt catcaaacaa tatatcgaag
     8221 cgtctggcaa tcaggtctat tttacctggc aaggcggtga acccactctg gctggcctgg
     8281 attttttccg taaagttatt cactatcaac aacgctatgc aggccaaaaa cgtattttta
     8341 atgcattaca aacgaatggc attttattga ataatgaatg gtgtgccttt ctcaaagaac
     8401 atgattttct ggttggtatc tcgatcgatg gcccccagga gttacatgac cgttacagac
     8461 gcagtaattc aggtaacggt acttttgcaa aagtgatagc agccatcgag cgtctgaaat
     8521 catatcaagt agagtttaat acgttaaccg tcattaataa cgttaatgtc cattaccctc
     8581 ttgaggttta tcatttttta acatctatcg gcagtaaaca tatgcaattt atcgaattgc
     8641 tagaaaccgg gacgccgaat attgatttca gtggtcatag tgagaacaca ttccgtatca
     8701 ttgatttttc tgtgcctccc acggcttatg gcaagtttat gtcaaccatt tttatgcaat
     8761 gggttaaaaa cgatgtgggt gaaattttca ttcgtcagtt tgaaagcttt gtcagccgtt
     8821 ttttggggaa tgggcataca agttgtattt tccaggagtc ctgcaaggat aatctggttg
     8881 ttgaaagtaa tggagacatt tacgaatgcg accattttgt ctatccacag tacaaaattg
     8941 gaaacattaa taaatctgaa ctcaaaacga tgaacagtgt acaactgaca gcgcaaaaaa
     9001 cggatttcag cgaaatgtca gcaatgtgca tataaaccta tctgcaatgg cggttgtcct
     9061 aagcatcgta ttactaaagt aaacaatgag actgtttctt atttttgcga aggttataaa
     9121 atcctttttt caaccatggt accttatatg aacgccatgg ttgagttagc taagaacaga
     9181 gtaccgcttt accacattat ggatgttgca aaacaaatgg agaataatta ataacgctct
     9241 cttttaactc cattcttagc ctccttctta tgaccttaac aaggcacccg acataaattc
     9301 ctgtcgggtg tttcgtcatc atcatcttcc cttgcgaaaa tcctggctta cgtggtatgc
     9361 gtcagggcat tagcaatacc cttgattatc aatagcataa ccataaccac tgaaaattaa
     9421 aaatttctat atatttcata ttgttaaatg atacttaaat aaatttcatc agtaaatcat
     9481 tgccatagat aatcattgga tggcacaatc aattttcgct aatgataacc attcccatta
     9541 cgttttgtat gttgatagca aagtatctct gtctgctgaa gcccttttgg ctgcgtaaga
     9601 ataataaaac ttcagtgttg ttaatcatta ttatcctcgc aatgatcctt ggcgtggtta
     9661 aaattcaggt ctggttaaac gactggaata atgatttttt caatgcgcta agccagaaag
     9721 aaaccgataa gctttggcag cttgttcttt ggttccctgc cctgctgggg atctttgtat
     9781 taatatccgt aaacaaaaca tggttaatta aactactgac tattcgctgg cgcgaatggt
     9841 taacagatta ctaccttaat cgctggtttg cagataaaaa ctactacttc acgcagatct
     9901 acggcgagca taaaaataca gataaccctg accaacgtat cgctgaagac attctcttac
     9961 tgatcaacaa aacgctcagt ctttcatttg gcttcatcca gtcacttagc atgctgatca
    10021 cctttaccgt tattctttgg caaagcgcgg gtacgctctc attcactgtt ggtggaacag
    10081 aatggaatat ccaggggtat atggtctata ccgtcgtgct cattgtgatc ggtggaactt
    10141 tatttaccca taaggtgggt aaacgtattc gtccgcttaa tgtggaaaaa caacgtagcg
    10201 aagcgacatt tcgaactaat cttgtgcagc ataataagca ggcagaactg attgcgctaa
    10261 gcaatgctga gtctctacaa cgccaggagt tgcgcgacaa ttttcatacc attaaagaga
    10321 actggcatcg tttaatgaat cgtcagcggt ggcttgatta ctggcaaaat atttattcgc
    10381 gctcgcttag cgttcttccc tactttctgt tattgccgca gtttattagc gggcagatca
    10441 atctgggcgg actgatgaaa tcgcgccagg catttatgct agtatcgaac aatttaagct
    10501 ggtttattta taaatatgac gaacttgctg aactggctgc ggttatcgat cgcttgtatg
    10561 agttccatca actcactgaa tagcgcccta cgaataagcc taaaaattgc caacatgcgg
    10621 tacaagtggc taatgcgagt attcgtacgc ctgataataa gatcatatta gagaacttga
    10681 actttcatgt ttcgccaggc aaatggctat tactgaaagg ctactctggt gcgggaaaaa
    10741 ccacactgct taaaacatta tcccactgct ggccgtgatt taaaggtgat atttcttctc
    10801 ctgctgacag ttggtatgtg tcacaaacac cgttaatcaa aagcggctta ctgaaagaga
    10861 ttatttgtaa agcacttccc ctgcccgtag acgataaatc gttgagcgaa gtactgcatc
    10921 aggttggtct ggggaaattg gctgcgcgta ttcatgacca cgatcgctgg ggagatattc
    10981 tttccagcgg cgaaaaacaa cgtatcgccc tggcacgatt aattttacga cgtccgaaat
    11041 ggatatttct tgacgaaact acctctcatc ttgaggaaca agaggctata cgcttactgc
    11101 gtttagtgcg tgaaaaacta cccacaagcg gcgtcattat ggttacacat caacccggtg
    11161 tctggaacct ggctgatgat atttgtgaca ttagcacggt tttataacaa caataaaagc
    11221 cctgctgtac ggagatataa ataaatgaag cgagttctta ttcctggcgt cattttatgt
    11281 ggcgctgatg tggcgcaggc cgtcgatgac aaaaacatgt acatgcattt ttttgaagag
    11341 atgacggtct atgctcctgt ccctgtaccc gtaaacggca acacgcatta caccagtgaa
    11401 agcatcgagc gtttaccgac cgggaatggc aatatcagcg atctgctgag aaccaaccct
    11461 gcggtacgca tggattcaac gcaaagtacc tcgttgaacc agggagatat tcgccctgag
    11521 aaaatctcta ttcacggtgc gtcgccctac cagaatgcct atttgattga cggtattagt
    11581 gcaactaata acctgaaccc agcgaatgag tccgatgcca gtagtgcaac caatattagc
    11641 gggatgtcac aggggtatta tcttgatgtc agcttactgg acaatgtgac gctttatgac
    11701 agttttgtgc cggttgaatt tggtcgcttc aatggcgggg taattgatgc aaagatcaaa
    11761 cgcttcaacg ctgatgatag caaggtgaaa ttgggttatc gcactacgcg tttggactgg
    11821 ttaacatcgc atatcgatga gaataacaag agcgcattta atcaaggttc ttcaggaagt
    11881 acctatttct ctccagattt taaaaagaac ttttatacct tgtcgtttaa tcaggagctg
    11941 gctgataact ttggcgttac cgccggttta tcgcgccgcc agtctgatat cacccgcgcg
    12001 gattatgttt cgaatgacgg cattgtcgcc ggtcgggcac agtataaaaa cgttatcgat
    12061 actgcattga gcaaatttac ctggtttgcc agcgaccgct ttacccacga tttaacctta
    12121 aaatataccg gctccagccg tgattataat accagcacct tcccgcaatc tgatcgcgaa
    12181 atgggtaata aatcctatgg tctggcatgg gatatggata cgcaactcgc atgggccaaa
    12241 ctacgtacca ccgttggttg ggatcatatt agtgattata cccgtcacga tcatgacatc
    12301 tggtacaccg aactttcatg tacatatggt gatattacag ggcgttgtac ccgtggcgga
    12361 ttaggacaca tttcccaggc tgtagataat tacaccttca aaacacgcct ggactggcaa
    12421 aaattcgccg tgggtgatgt ttcgcatcaa ccctacttcg gcgcggaata catctattcc
    12481 gatgcatgga ctgaacgcca taaccagtct gaatcctatg tgattaatgc tgcaggaaag
    12541 aaaactaacc ataccattta ccataaaggt aaaggcagcc tgggaattga caactacacg
    12601 ctgtatatgg cggatcacat tagctggcgg aatgtgtcgt taatgcccgg tgtgcgttat
    12661 gactatgaca actatctgtc aaaccacaat atctccccgc gctttatgac ggaatgggat
    12721 atttttgctg atcaaacctc aatgattacc gccggttata accgttacta tggcgggaat
    12781 attcttgata tgggattacg tgatatccgc aatagctgga cggaatcggt atcaggtaat
    12841 aaaaccctga cgcgttatca gaatttgaaa acgccttata acgatgaact ggcaatggga
    12901 ttgcagcaaa aaatcgataa gaacgttatt gcacgcgcaa actatgttta ccgtgaagcg
    12961 catgatcaaa tcagcaaaag cagtcgtacc gacagcgcga ctaaaaccac cattactgaa
    13021 tataacaacg atggcaaaac caaaacgcat tcgtttaacc tcagttttga actggccgaa
    13081 cccctgcata tccgccaggt agatattaac ccgcaaattg tctttagcta tatcaagagc
    13141 aagggcaact tgtcgttaaa caatggttat gaggagagca ataccggtga taaccaggtg
    13201 gtttataacg gtaatctggt ctcttacgat agcgttccag tggcagattt taatacccca
    13261 ttaaagatct ccttaaacat ggatttcacg catcaaccga gcgggttagt gtgggcgaat
    13321 acgctggcct ggcaagaagc gcgtaaagct cgcattatcc ttggtaagac aaatgcgcaa
    13381 tacatcagcg aatattcaga ttacaagcag tatgttgacg aaaaactgga tagcagcctg
    13441 acctgggaca cccgcttgtc ctggacgcca caatttctga aacaacaaaa cctgacgatc
    13501 agtgccgata ttctcaatgt actggatagc aaaaccgctg ttgatacaac gaacaccggt
    13561 gtggcgacct acgccagtgg ccgtactttc tggcttgatg tcagcatgaa attttaatag
    13621 ttcggatttt acttaaccgg gtaaattcgc ccggttttcg catggagata attatgagaa
    13681 acctctgttt cttactgacg ttagtggcaa ctctgttgct ccccgggcgg ctgattgccg
    13741 ccgccttaca gcaggatgaa aagttaatta ccgggcaact ggacaatggc ttgcgatata
    13801 tgatttatcc gcatgctcat ccaaaggatc aggtaaattt atggctgcaa attcataccg
    13861 gttcattgca ggaagaagac aatgagcgcg gcgtggctca ttttgtagaa catatgatgt
    13921 ttaacggcgc aaaaacatgg ccgggtaata aagtcatcga aacatttgag tcaatgggcc
    13981 tgcgttttgg tctcgatgtt aatgcctata ccagctatga cgaaacggtg tatcaggtga
    14041 gtttgccgac tacgcagaaa caaaatctgc aacaagtgat ggcaatcttc agtgaatgga
    14101 gtaatgccgc aacctttgaa aaactcgaag tagacgctga acgtggcgta atttctgagg
    14161 aatggcgtgc ccatcaggat gcgaaatggc gcacctctca ggcgcgccgc cctttcctgc
    14221 tggctaatac ccgtaattta gaccgtgaac ctatcggcct gatggatact gtcgccacgg
    14281 tcacaccggc acaattgcgc caattttatc aacgctggta tcaaccaaat aatatgacct
    14341 ttatcgtggt cggcgatatc gacagtaaag aagcgctggc gctgataaag gataatttaa
    14401 gtaagtttcc ggctaacaaa gcagctgaaa atcgcgtctg gccgacaaaa gccgaaaacc
    14461 acctgcgctt taatatcatt aatgataaag aaaaccgggt gaacggcatc gcactctatt
    14521 atcgcctgcc aatggtacaa gtgaacgatg agcaaagctt tgtcgaacaa gctgaatgga
    14581 gcatgttagt tcagctgttc aatcaacgtc tgcaggaacg catacagtcg ggcgagttga
    14641 agactatttc tggcggcact gcgcgcagcg ttaaaattgc acccgattat cagtcgctgt
    14701 ttttccgtgt aaatgcacga gacgataata tgcaggatgc tgcgaatgca ttaatggcag
    14761 agttggcaac cattgatcag catggttttt ctgctgaaga actcgatgat gtcaaatcta
    14821 cccgcctcac ctggctgaaa aatgcggttg atcagcaagc tgaacgtgat ttacgtatgc
    14881 tgaccagtcg cctggcatcc agctcattaa ataatacgcc gttcttgtcg ccggaagaga
    14941 catatcaact ttcgaaacgt ctgtggcagc aaattaccgt gcaaagtctg gcggaaaaat
    15001 ggcagcagtt aagaaagaac caggacgcat tttgggagca aatggtaaac aatgagcttg
    15061 ccgccaaaaa agcattgtct cctgcggcta tcctggcgct ggaaaaagag tacgccaaca
    15121 aaaagctggc ggcttacatc ttcccaggca gaaatttatc gttaacagta gacgctgacc
    15181 cacaggcgga aattagcagc aaagaaacgc tggctgagaa tctgacatca ttaacacttt
    15241 ccaatggtgc caaggttatt ctggcaaaat ccgcgggtga agagcaaaag ctacaaatta
    15301 ttgccgtatc gaataaaggc gatttaagtt tccctgcgca gcaaaaatca cttatcgcgc
    15361 tggcaaataa agcagttagc ggaagcggcg ttggcgaact ctcctcttcc agcctgaaac
    15421 gctggagtgc ggaaaactcg gtaaccatga gcagtaaagt cagtggcatg aatacgttgc
    15481 tctccgttag cgcgcggact aataaccctg aacctggttt cctgttgatt aaccagcgaa
    15541 tcacccacag cacgattaac gacaatattt gggcatcgct acaaaatgct caaattcagg
    15601 cgttgaaaac gctcgaccag cgtccagcgg agaaattcgc ccagctgatg tatgagacgc
    15661 gctatgctga tgaccgcacg aaattactgc aagaaaatca gattgcacag tttactgccg
    15721 cagatgcgct ggctgccgat cgccagttgt tttcatctcc agcggatatc acgtttgtca
    15781 ttgtcggtaa tgtcgcagaa gacaaactcg tggcgttaat tacgcgttac ttaggctcaa
    15841 tcaaacactc tgattcgcca ttagccgcag gtaaaccatt aactcgcgcg acggacaacg
    15901 catcggttac tgtaaaagaa caaaatgaac ctgtggcaca ggtttcacag tggaagcgtt
    15961 atgattcccg gacacctgtt aatctggcga cgcgtatggc gctcgatgct tttaacgtcg
    16021 cactggcaaa agatctacgc gttaatattc gtgaacaggc atctggggca tacagcgttt
    16081 cttctcgcct ctcggttgat cctcaggcta aagatatcag tcatttgctg gcttttactt
    16141 gtcaaccaga acgacatgat gaacagttaa cgttagcgaa tgaagtgatg gttaagcgtt
    16201 tgactaaggg gatcagtgag caagaactga acgaatacca gcaaaacgtt cagcgcagcc
    16261 tcgatatcca acagcgtagc gttcaacaat tagcgaacac tattataaat agtcttattc
    16321 aatatgacga tcctgcagca tggactgagc aggagcaatt gttgaaacaa atgacgatag
    16381 agaatgttaa cactgccgtt aaacaatatc tttctcatcc ggtaaatact tataccggag
    16441 tattattgcc aaaataataa cagccccgtc aacacatcgt tggcggggat tttagcaata
    16501 ttcgctattt ttatgtaata attttataaa tgcgttcaaa ataataacca agtactaata
    16561 gtgatatttt aatgtctgat ttttacgtga taattcagga ggcacagaaa gcgcataaaa
    16621 ataacaacat aaaacacctt gccaccaccc aaaaattctc tattgcattg tttttaatga
    16681 aaaaaatatc attccaacaa tatctcacga taaataacat taggattttg ttatttaaac
    16741 acgagtcctt tgcacttgct tactttatcg ataaatccta cttttttaat gcgatccaat
    16801 cattttaagg agtttaaaat ggataagaag caagtaacgg atttaaggtc ggaactactc
    16861 gattcacgtt ttggtgcgaa gtctatttcc actatcgcag aatcaaaacg ttttccgctg
    16921 cacgaaatgc gcgacgatgt cgcatttcag attatcaatg atgaattata tcttgatggc
    16981 aacgctcgtc agaacctggc cactttctgc cagacctggg acgacgaaaa cgtccacaag
    17041 ttgatggatt tatccattaa caaaaactgg atcgacaaag aagaatatcc gcaatccgca
    17101 gccatcgacc tgcgttgcgt aaa
//
LOCUS       DAJVQH010000080        17099 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJVQH010000080 DAJVQH010000000
VERSION     DAJVQH010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.080"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(25..1053)
                     /gene="epmB"
                     /locus_tag="OQM87_002706"
     CDS             complement(25..1053)
                     /gene="epmB"
                     /locus_tag="OQM87_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS1508781.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1095..1661
                     /gene="efp"
                     /locus_tag="OQM87_002707"
     CDS             1095..1661
                     /gene="efp"
                     /locus_tag="OQM87_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS1508782.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1713..1838
                     /gene="ecnA"
                     /locus_tag="OQM87_002708"
     CDS             1713..1838
                     /gene="ecnA"
                     /locus_tag="OQM87_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS1508783.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            1949..2095
                     /gene="ecnB"
                     /locus_tag="OQM87_002709"
     CDS             1949..2095
                     /gene="ecnB"
                     /locus_tag="OQM87_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS1508784.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2271..2500
                     /locus_tag="OQM87_002710"
                     /pseudo
     CDS             2271..2500
                     /locus_tag="OQM87_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2497..3030)
                     /gene="blc"
                     /locus_tag="OQM87_002711"
     CDS             complement(2497..3030)
                     /gene="blc"
                     /locus_tag="OQM87_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1508785.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3119..4252)
                     /locus_tag="OQM87_002712"
     CDS             complement(3119..4252)
                     /locus_tag="OQM87_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1508786.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            complement(4315..4674)
                     /gene="frdD"
                     /locus_tag="OQM87_002713"
     CDS             complement(4315..4674)
                     /gene="frdD"
                     /locus_tag="OQM87_002713"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1508787.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4685..5080)
                     /gene="frdC"
                     /locus_tag="OQM87_002714"
     CDS             complement(4685..5080)
                     /gene="frdC"
                     /locus_tag="OQM87_002714"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1508788.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5091..5825)
                     /gene="frdB"
                     /locus_tag="OQM87_002715"
     CDS             complement(5091..5825)
                     /gene="frdB"
                     /locus_tag="OQM87_002715"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1508789.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(5818..7626)
                     /gene="frdA"
                     /locus_tag="OQM87_002716"
     CDS             complement(5818..7626)
                     /gene="frdA"
                     /locus_tag="OQM87_002716"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1508790.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            7951..8928
                     /gene="epmA"
                     /locus_tag="OQM87_002717"
     CDS             7951..8928
                     /gene="epmA"
                     /locus_tag="OQM87_002717"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCS1508791.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9147..10649
                     /gene="yjeM"
                     /locus_tag="OQM87_002718"
     CDS             9147..10649
                     /gene="yjeM"
                     /locus_tag="OQM87_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCS1508792.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(10800..14111)
                     /gene="mscM"
                     /locus_tag="OQM87_002719"
     CDS             complement(10800..14111)
                     /gene="mscM"
                     /locus_tag="OQM87_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCS1508793.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSIAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(14133..15101)
                     /gene="psd"
                     /locus_tag="OQM87_002720"
     CDS             complement(14133..15101)
                     /gene="psd"
                     /locus_tag="OQM87_002720"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCS1508794.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(15198..16250)
                     /gene="rsgA"
                     /locus_tag="OQM87_002721"
     CDS             complement(15198..16250)
                     /gene="rsgA"
                     /locus_tag="OQM87_002721"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCS1508795.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNIKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDG"
     gene            16345..16890
                     /gene="orn"
                     /locus_tag="OQM87_002722"
     CDS             16345..16890
                     /gene="orn"
                     /locus_tag="OQM87_002722"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCS1508796.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         3927 a         4426 c         4496 g         4250 t
ORIGIN      
        1 tgtttaactt ccctgtttaa tcagttactg ctggcgtagc tggagatcca gcggcgtttt
       61 gctgggttcg ccgccaattt ctcgcgccag tttcggcacc agatatcccg acaccaatgt
      121 cagcaactca cgcataatct gccgtgcttc gtcatcactc accataaaat gcgccgcgcc
      181 ctgtacttta tctagcacat gcaggtaata cggcatcacg ccggcatcga acaacgcatt
      241 actcaggttt gccaacgttt gtgcgttatc gttcacacca cgtaacagaa cgctctggtt
      301 aagcagggtg acaccgacac ggcgcaattt agccatcgcc tgacggaacg tttcatctac
      361 ctcattggca tgattgatgt gattcaccag caagatttgc agcgtagaac gggcaaagcg
      421 ttcaaccagc gcctcggtga tacgtgccgg gatcacaatc ggcagacggc tgtgaatccg
      481 cagacgtttg atatgcggga tggcttccag ttgtgtgagc aaccagtcca gctcgtgatc
      541 tttcgccatc agcggatcgc cgccggagaa aatcatctcg tccagttccg gatgcgcagc
      601 aacatactca agtgcagtct gccagttacg cttgttgccc tgattttcgg cataggggaa
      661 gtggcgacgg aagcaatagc ggcaatttac cgcgcagccg cctttgacca gcaaaagcgc
      721 ccggttgtgg tatttatgca acaaaccagg cactacgctg tgctgttctt ccagtgggtc
      781 ggtggagaat ccggacgcga cgacaaactc atcttgcgag gtaagtacct gacgcaaaag
      841 aggatcgtcc ggattgcctt tctccatgcg atcgataaat gagcggggca cacgcagggc
      901 aaacagcttt ttggcgctgc gtccggccaa cagtttttcg tccgcgtcta tattcaaaag
      961 acgcagaagt tcatcaggat cggtcacaac atcggcaagt tgcgttaacc aatcttctct
     1021 ggatggggta tttagggtta caatatgcgc cattttgtgg cttagctacc aattaacaaa
     1081 tttcagaggg ccttatggca acgtactata gcaacgattt tcgtgctggt cttaaaatca
     1141 tgttagacgg cgaaccttac gcggttgaag cgagtgaatt cgtaaaaccg ggtaaaggcc
     1201 aggcatttgc tcgcgttaaa ctgcgtcgtc tgctgaccgg tactcgcgta gaaaaaacct
     1261 tcaaatctac tgattccgct gaaggcgctg atgttgtcga tatgaacctg acttacctgt
     1321 acaacgacgg tgagttctgg cacttcatga acaacgaaac tttcgagcag ctgtctgctg
     1381 atgcaaaagc aattggcgac aacgctaaat ggctgctgga tcaggcagag tgtatcgtaa
     1441 ctctgtggaa tggtcagccg atctccgtta ctccgccgaa cttcgttgaa ctggaaatcg
     1501 ttgataccga tccgggcctg aaaggtgata ccgcaggtac tggcggcaaa ccggctaccc
     1561 tgtctactgg cgctgtggtt aaagttccgc tgttcgtaca aatcggcgaa gtcatcaaag
     1621 tggatacccg ctctggtgaa tacgtctctc gcgtgaagta atgcggttgt ggtgcggcct
     1681 gcaggctgca ccatcactta ttcaggtcag agatgatgaa acgccttatc gttcttgttt
     1741 tgcttgccag cacgctgctc acgggctgta acaccgcttg cggtttcggc gaagacatca
     1801 aacatctcgg caactccatc tctcgcgctg ccagctaatt tttcttctct tccgaaaaat
     1861 catcagattt ccatcatttt tggcgatgtt gtctattatt aatttgctat aggcaaacat
     1921 aaataacatt acctaaaagg aagacgttat ggtgaagaag acaattgcag cgatcttttc
     1981 tgttctggtg ctttcaacag tattaactgc ctgcaacacc acgcgtggcg ttggtgaaga
     2041 catttctgat ggcggtaacg cgatttctgg cgcagcaacg aaagcgcagc aataagcaat
     2101 aacggtacga cagcagtgtc gtgccgtttg ttttttctgc gatcgtcata aaggtaatag
     2161 ttgaaattcc cctgccacct ggcaaaatat ccgttcaacc atcagctttg caggacgacc
     2221 tgcaaacgcc tcttttcacc ggggacggcc ccaaatttcc ggagcctgat atgtcctgga
     2281 ttatcttagt tattgctggt ctgctggaag tggtatgggc cgttggcctg aaatataccc
     2341 acggctttag ccgtttgatg ccgtgtggac gggtattggc gcagtcggcg cggccatcac
     2401 cggcattgtg ctgctcggtg agtccgctaa cccgatgcgt ctggcgagtc tggcgttaat
     2461 cgtattgggg attattggtc tgaaactcag cactcactaa ctaccgggct gctgtaccca
     2521 aataaactta ctgacatcaa acccttcccg ggtcgcgact gccagcatct cctgtttcac
     2581 ttcgtcagaa atggttggcg tgcgggagag tatccacagg tagtcgcggt ccgggccgca
     2641 aaccagcgca tggcggtatt cccgatcgag tgcaataacg ttataaccgc catagaaagg
     2701 accaaagaat gacactttca gcgcagcgcg agttggtgcg ccggtaaagt acgctttccc
     2761 ttcactctgc tgccacattc ctctgtcagg gttatagcct ttattaatga cattcaggcc
     2821 gccgtcatca cgcaggctgt atgttgcggt gactttttcc agtccacgtt caaaacggtg
     2881 atcaaaacgg gcaatctcat accaggtacc gagataacgt ttggcgtcga aattattcac
     2941 tacggtcacg ccacgcggcg gcgtaggaga actacaggca acgaccagaa atgcagctgt
     3001 cgctgcggca acgagaggga gcaggcgcat aaatgtttcc ttactggttt ttttctaagt
     3061 gtagatgaca gcaaggaaaa gcgcagaaaa ggtccgaaaa ttcggacccg atgaaatttt
     3121 actgtagagc gttgagaatc tgacaggcgg cggcgactct ctctggattg ggatagtttt
     3181 tgttagccag catcacgata cccagctctt tttctggaat aaacgcgaca tagctaccaa
     3241 atccgccggt cgcccccgtt ttatgtaccc atgatgcgcg tactgcagga gttgggggct
     3301 taatcgcttt taccgggcgt gctgccagtg caattttatt gtcactgccg ttaatgatgc
     3361 tgtcaggatt gaccggccag tccagcattt cccagcccag gccctgatac atatcgccgg
     3421 tttgccagta gcgagattgt gccagttgta tcccttgttg aagcgttttc tcattgatat
     3481 caaggggggg taaattgctt tgcacccagc gggccatatc ttcaatggtc gacttcacac
     3541 cataagcttc agcatctaac gcccctggcg aaacatgcac tgccttacct tcgcgatatc
     3601 cccaggcgta attcttttct tctgggggag gtacattaat ccacgtatgg ttgagtttga
     3661 gtggctggaa gacacgagtt tgcatcgcct gctcaaaact caaaccagac ggcttcacag
     3721 ccagtgcgcc gaacaaaccg atactggagt tggcatacag acgttgtgtt cctggggccc
     3781 atgcaggctg ccagttttga tagaagcgca gcaagtcgct tgaggatttc acttcatccg
     3841 gcacctgcaa tggcaggccg ccagcggtgt aggtcgcaag atgtaatagt gtgatcccat
     3901 tccactgttt agcggtaagt tcaggccagt attttgttgc gggatcgctt aacttgattt
     3961 cccctcgagc aatagcgtcg ccaccaagca cgcccgtaaa tgttttgctg accgaaccta
     4021 actcaaacaa cgtttgctgt gtgacgggct gctttttggc gatgtccgca tagccccaag
     4081 taaagtaata aggtttaccc tgataaatta ccgccaccgc cataccgggg atcttttgtt
     4141 gctctataag cggggtaatt gtgcgatgca caatatcgtt gatttgttga ggggcagcaa
     4201 atgtggagca agaggcggta attaataagg cgcagagcgt cgttttgaac ataaggtctg
     4261 gtttccatac aaaacggccc gccataggcg ggccggattt acattggcga tgcgttagat
     4321 tgtaacgaca ccaatcagcg tgacaactgt caggatagca gccagaccgt agaaaaccca
     4381 tttgcccgca ggcacgtgga ttttcagatc gtgcatcgcg tggtgcatac ggtgtaaacc
     4441 acaccacagc ggcagaacga tcatcaggaa caggaatacg cgaccaatga agctctgcgc
     4501 gaacgccaga acgcgctcgt agctcagcgc atcacccgga aacagcccca gtggcagcag
     4561 aatacccacc agcaggatca tcaccggcgc aataattgcg ctccacatac caccggcccc
     4621 gaagaggccc cagaataccg gttcgtcaga acgctttgga tttggattaa tcatctcagg
     4681 ctccttacca gtacagggca acaaacagga ttacgatggt ggcaaccaca gttaccgccc
     4741 agagactttt gataattggc tctggtccca ttttttcgtc ttttacaatg atattggccg
     4801 ctttcggtgc cagttcaaac caggttttgg tgtgcagcag agctgccgtc agagtgatca
     4861 ggttaatgat cacgataacc gggttttgta aaaagtcgac gaatcccgcc caggcttccg
     4921 ggccattttt cagggcaaac agcccgaaaa tcagttcaat gctgaaccac acagccggaa
     4981 ccgccgtgcc ttcgcgcagc atgtaaaagc gataaaacgg caattttttc caccaggtgg
     5041 acgtcattgg ccgtacatac ggtttacgtt tagtcgtcat gttgcactcc ttagcgtggt
     5101 ttcagggtcg cgataagaaa gtctttcgaa ctttctactt tgccctgctg aatggccgca
     5161 gccggatcga cgtgtttcgg gcagacttcg gagcaatagc ccacgaaagt acagctccat
     5221 acaccgttct ggctgttcaa ctgcgccata cgctccttct taccgtggtc gcggctatct
     5281 tcgttataac gatgcgccag cgtaatggca gccggaccga tgaactctgg gttcaggcca
     5341 aactgcgggc acgcggcgta gcacagacca cagttgatgc aaccggagaa ctggtgatac
     5401 tttgccatct gcgccggggt ctggatgtta gtaccctgat ccgcggtgcg ggagttgccg
     5461 atgatgtacg gtttgatcgc ttccagactt tcgatgaagt gggtcatatc gaccaccaga
     5521 tcgcgttcaa tcgggaagtt agctaacgct tcaaccttca taccgtcggt gtaatcacgc
     5581 aggaaggttt tacatgccag ttttggcacg ttgttaacca tcatgccgca ggaaccacaa
     5641 atcgccatac ggcaggacca gcggtaactc aggtccggtg ccaggttgtc tttgatgtag
     5701 cccagcgcat ccagtaatga ggtagttgcg tcataaggca cttcatagaa tgcgctatgc
     5761 ggtgcggtat cgacttccgg gttatagcgc accacctcaa ttttcaggtt tttcatctca
     5821 gccattcgcc ttctccttat tattggctgc ttccgcctta tcggctgcat ccgcttcgcc
     5881 accgtaaacg cgtttagctg gcggcagcgt agtaatcttc acgtcgctgt actccaggcg
     5941 agtcgtgcca tcagcatcgc ggaaggcgag ggtgtgtttg agaaagttga cgtcgtcacg
     6001 ctcggtgcaa ccttcgtcca gacgctggtg tgcgccgcgg gactctttac gtgccattgc
     6061 ggagtgcgcc atacattcag caacgttcag accgtggctc agttcaatgg tgtagagcag
     6121 gtcggtgttg aacacgctgg aggtgtcggt gatgcgcacg cgcttgaagc gttcctgcag
     6181 ctctgccagc ttgtcgatgg ttttctgcat cagttccggc gtacggtaga taccgcaacc
     6241 ttcttccatt gccaggccca tttcgtcgcg gatcttcgcc cagttttcgc cgccatcctg
     6301 gttaaccaga tctttcagac gttgttcaac gccagctgcc tgcgcttcaa ttgccgcttc
     6361 gttgccatta ccggcagttg ctgcacgctc tgtcgcttgt tcaccggcca gacggccgaa
     6421 gaccaccagt tccgccaggg agttggagcc cagacggttt gcaccgtgca gaccaacaga
     6481 ggaacattca cccacggcga acagaccttt aatgcgggtt tcacagttct gatcggtttc
     6541 gataccgccc atggtgtagt gtgcagtcgg acgtaccgga atcggttctt taaccggatc
     6601 gacgccaacg tacgctttcg ccagttcgca gatgaacggc agacgttcat gcagtttttt
     6661 ctcgccgagg tgacgcaggt cgagataaac cacatcacca cgcggcgtgg agatggtgtt
     6721 gcctttacgc cattcgtgcc agaaggcctg agaaactttg tcgcgtggac ccagttccat
     6781 atatttgttt ttcggctcgc ccagcggagt ttccgggccc atgccgtaat cttgcagata
     6841 acggtagcca tttttgttga ccagaatacc gccttcacca cggcagcctt cggtcatcag
     6901 gataccggaa cctggcagac cggttgggtg atactgaacg aattccatgt cacgcagcgg
     6961 aacgccgtgg cttagcgcca tacccatacc gtcaccggta acgatgccgc cgttggtgtt
     7021 gtaacgataa acgcgacccg caccgccggt agccataacg accgcgttag cacggatctg
     7081 caccaacgtg ccttccatca tgttcattgc taccaggccg cgaacatgac catcatcaac
     7141 cagaatatcc agcacgaaat gttcgtcaaa acgctggatc tgcgggaatt gcagagaggt
     7201 ctggaacagc gtgtgcagca tatggaagcc ggtcttatcg gcggcgaacc aggtgcgctc
     7261 gattttcatg ccgccgaagc gacgtacgtt gacgctacca tccgggcgac ggctccatgg
     7321 gcatccccac agttccagtt gggtcatttc ggttgggcag tggtggacga aataatccac
     7381 gacatcctgc tcacacaacc agtcgccacc cgctactgta tcgtgaaagt gatattcgaa
     7441 gctgtcatga tcctgcgcga cagcggcgga gcccccttct gcagcaacgg tatggctacg
     7501 catcgggtat acttttgaga ttagtgcgat ttttgcattt ggatttgcct gcgcggcagc
     7561 aattgcagca cgtaatcccg cgccaccggc gcctacaatg gcaagatcgg cttgaaaggt
     7621 ttgcacgaca ttcctccaga ttgtttttat cccacagccc cgtacttcag ggtaagtgcc
     7681 tgaaagttac ggtctgcgaa cgctattcca ctgctccttt ataggtacaa cagtatagtc
     7741 tgatggataa gtctgaaatt tgacgagatc gattttttta gtgcgcaaga gggctaaatt
     7801 atcactgaag atgattaatt taattactaa accatcagat cgtgcttttt ttagtcactg
     7861 cccaccgctg tttgattcct gcgtaaaatt tgtctcgccg tcgcgttgcg agtagacttc
     7921 gtgcccttgt caaaaactgg agatttaact atgagcgaaa cggcatcctg gcagccgagc
     7981 gcatccattc ctaacttatt aaaacgcgcg gcgattatgg cggagatccg tcgtttcttt
     8041 gccgatcgtg gagtgctgga ggtggagacg ccttgtatga gccaggcgac ggtaaccgat
     8101 attcatttgg tcccgtttga gacacgtttc gttggccccg ggcattcgca ggggatgaat
     8161 ctctggttaa tgaccagccc ggaataccat atgaaacgcc tgctggttgc tggttgtggg
     8221 ccggtattcc agctgtgccg cagcttccgt aatgaagaga tggggcgtta tcacaaccct
     8281 gagttcacta tgctggagtg gtatcgaccg cactatgata tgtaccggtt gatgaacgag
     8341 gtggacgatc tcttacaaca ggtgctggac tgcccggcag cagaaagcct ttcttatcaa
     8401 caagctttct tgcgttatct ggaaattgac ccgctctctg ccgacaaaac gcaactgcgg
     8461 gaagtcgcag cgaaactgga tttgagcaat gttgctgata ccgaagaaga ccgcgacacg
     8521 ttgctacaat tgctgtttac ctttggcgta gagccaaata ttggtaaaga aaaaccgacc
     8581 tttgtgtacc actttccagc cagccaggca tcactggcgc aaatcagtac cgaagatcat
     8641 cgggtcgctg aacgctttga ggtttattat aaaggtattg agctggcgaa tggtttccat
     8701 gaattaacgg atgcccgtga gcagcaacaa cgctttgaac aagataaccg taagcgcgcg
     8761 gcgcgcggtt tgccgcagca ccccattgac cagaatctga ttgaagcctt gaaagtcggt
     8821 atgcctgact gctccggcgt ggcattaggt gttgatcgtc tggtgatgtt ggcgctgggc
     8881 gcggagacac tggctgaagt catcgccttt agcgttgacc gggcataatt ctgaaaaatt
     8941 actgaattaa cagcgaagaa tggcgtgata agcgccattc tgtagcatat gttttcattt
     9001 ttccgttaaa ggccacaatt taagactgga tatttgatat catcaaggta tcaattctgt
     9061 attggttttt acgctgcctg ctcaatgttg cgcagtgcag ttcagtgggc acgtgttata
     9121 cacgcgctga aatgaaggat ggtttcatgc ctcacacgat aaaaaagatg agtctgatag
     9181 gactcatatt gatgatcttt acttccgtat ttggatttgc caatagccca tcggcttatt
     9241 acttaatggg ttatagtgcg attccctttt atatattttc tgcattgtta ttctttattc
     9301 cattcgcctt aatgatggct gaaatgggag ctgcttatcg caaagaagaa ggtggtatct
     9361 attcctggat gaataatagt gtcggaccac gttttgcctt cattggtacg tttatgtggt
     9421 tttcctctta tatcatctgg atggtgagta cctccgcgaa agtttgggta ccgttctcaa
     9481 cattcctcta tggtagtgac atgacccagc actggcgtat tgccggactg gagcctacgc
     9541 aggtggttgg tctgctggca gtagcatgga tgattctggt caccgtcgtt gcttcaaagg
     9601 ggattaataa aattgcccgc attactgcgg tgggcggtat tgcagtaatg tgtctgaatt
     9661 tagtattgct gttagtaagc attactattt tgttattaaa tggtgggcat ttcgcgcagg
     9721 atattaattt ccttgcatca ccgaacccgg gttatcagtc cggtctggca atgctatcgt
     9781 ttgtggtatt tgctattttt gcctatggcg gaattgaagc ggttggtggt ctggtcgata
     9841 aaacggaaaa tccagaaaag aactttgcca aaggtattgt ttttgccgct attgttattt
     9901 caatcggtta ttcgctggca atatttttat ggggcgtcag cacaaactgg cagcaggtat
     9961 taagtaatgg ttccgttaac cttggcaata ttacctatgt gctgatgaag agcctcgggg
    10021 tgacgctggg taacgcactg catttgtcac ctgaagcgtc attgtcgctg ggcgtatggt
    10081 ttgcgcgtat taccggactt tcgatgttcc tcgcttatac cggtgcgttc tttacgcttt
    10141 gctattcacc gctgaaagcc atcatccagg ggacgccgaa agcgttgtgg ccggaaccga
    10201 tgacgcgcct gaatgcgatg gggatgccgt ctatcgccat gtggatgcag tgcgggttgg
    10261 ttactgtctt cattctgctg gtttcgtttg gtggcggtac cgcatcggcg ttctttaaca
    10321 agctgacgct gatggcgaac gtgtctatga cgcttcctta cctgttcctc gcgctggctt
    10381 tcccgttttt taaagcacgt caggatctcg acagaccatt tgtgattttc aaaacgcgta
    10441 tgtcggcaat gattgcgacg gtggttgtcg ttctggtggt gacatttgcg aacgtcttca
    10501 ccattattca gcctgtggtt gaagccggag actgggacag cacattgtgg atgattggcg
    10561 gccctgtctt cttctcgctg ttagcgatgg cgatttacca gaactattgc agtcgcatgg
    10621 cgaataaacc tgagttagct ctcgactgat catatgaaat cctgcaactc atcgtctctc
    10681 cggcgatgag ttgtcaggaa gtaatgcgta tggatgatac tctccagggc cggtgataac
    10741 tgaagatgat gttaagctgc taaatgagcc acgttgacat catttggcag cttcatgcat
    10801 tacaaacttc ccgcctgacg acctttgccc gcagacgtca gcgttctccc cgtttgttta
    10861 ccgttgaggc tttccagacg catctggaag ggcgggaatg gcatatcgat accgtgggcg
    10921 tggaagcctg ccagaatcag ctggtggatc tcatggcgta gcggcatacg gtgacccatc
    10981 tcagcggcgt aaatacgcag ctcgaaaatc tgaatcccct gttgcagatc caccaggaag
    11041 acttccggtg ccgggttgtc gatcaccagc gagcagcgac gcgctgcggt gagcaggatt
    11101 tccgtcactt cttcactgtt ggcatcggca ggggccggaa tcgtcaacac tacgcgcgtg
    11161 accgagtcag agagcgacca gttgataaac tgctcggtaa taaacgcctt gttcggcacg
    11221 attatctctt tacggtccca gtcgctgatg gtggtggcgc gggtgttaat ttttgtcacg
    11281 ctaccagtaa gatcgcggat cgttaccgta tcgccaatgc gaatcggttt ttcgaacagg
    11341 atgatcaggc cagagataaa gttggcgaaa atttcctgca aaccaaaacc gagaccaaca
    11401 ccgagcgcgg caaccagcca ctgcaatttc gaccactcaa taccaatcat tgagaagccg
    11461 accagcccgc caatcagcat cagcagatat tttgtgatgg tggtgatggc gtaacccgta
    11521 cccggcgtta aatccaggtg ctgcaaaatc gccagttcca gcagcgcggg caggttgcgc
    11581 accagctgcg tggtgatgat aaataccaga atggcaatca gcaccgcacc gagggtaatt
    11641 ggctccagac tttctacgcc ctgtaccgtg gaggtgacat cccacagcga aatattttcg
    11701 aggaagccga aagcggaatg gatttctgac cacagcacaa tgaccgaaag cagggcgatc
    11761 aacatcaaaa ttgagcgcac cagccgcaag gattgcgcac tgatggcatc gagatcgact
    11821 tcgctttcat cgacttcaat tgctccttcc gggctactgt gatgatgcgc ttcctcttcg
    11881 ccacgcgcac gttgcgctaa catctctgcc cggcgatgct tcgcccggtc aaacgccagc
    11941 ctgcgacgct ggatcagcat ccagcggcgg ataacgtgat aaaccaccag tagcaggaac
    12001 cagatggcaa tcgaggtttc aagcctcgct aacagtgcct gcgccgttgc cagataaccc
    12061 accgccgacg ccagaatggc aaccagtggt gcgccaatca tcatgttcca cagcatatgg
    12121 ttggtaatgt tgtcgccgct gccttctttg ttgagataca acggaatacc ggcttttttc
    12181 aggcttaggg tgaccaccgc cagcgcaccg caaatgagga taaagcaaag ccgtcccagc
    12241 gaaccggaaa attcgcggtc gtcgaggtta tcgaacatca tcagcgccat aatcagcccg
    12301 atgctcatca ggtagtagcg catcccacgg gaaacacgtt cgcgcggcca gccaaaatga
    12361 gcgataaaca agccgttcgg gcgggcaaag gtggcgcaaa tcatcactac ccacagcagc
    12421 ggcacggtgg ccgttacacc atcgccaatc gcgaccgcca gcggataagg ccacgcctcg
    12481 cgcaagccgt aacccagcgt catccacagc accggtaacg gtgacgcgac gagaatcgac
    12541 cagaaaagag tgcgcaacgt cagccagaag tgatcctgag tcactttgcc gactttcgcc
    12601 gccgaacgtt caagaaaacg ggtgaaatag cggcgcgagt aaatactgca accgaccaga
    12661 atcaacgcgc caaacagcgg caaaattgtc tctttgctgg tcagcatcat cacactggct
    12721 ttgcccaact gactgaaggt gtccagcgaa atgagacgac gcagatcctg ggcgatttcc
    12781 agcggccagg cgatggtcat cgggcgcacg tcagaggtcc agaacagata gcggtgcgtt
    12841 gcttcgttca cctctttcag cgcatcctcc agttgcccgt tggagacttt cagcttggtc
    12901 agttccaata gtagcgtgtc gccaccctgc aacaatgagt tcagcaactc acgctgagtg
    12961 cgcagttgtg cttccagaat acggttttgc tcggcggtca gcggctgacc gtcggcctga
    13021 tggatttgcc gtagcagcgg ctgtttatta agtaaatcct cataacgtaa ccgttgcaca
    13081 cgcaactgcg ccatttcggt atcaagctgt tgtggttttg gcatttctgg tagccgtgcc
    13141 acctgcgccc gcagcgcttc gccgagcagg ttggacgatc ccagccattg cgactgttca
    13201 cgcagcgtat tcaacgcctg ccggacctgt aacgtctggc tggcagcctg acgctgttgc
    13261 gaggcaacga gctccatccg ctgcgcctgt tgattcagcg ctgccgatag ttcgcggtta
    13321 attttgaatt gcgcgacgat atctttcggc aaatcggcac tgttttctgc cagtaattcg
    13381 gtactttcca gcgcccgctc cgcctcaagc tgacgttggc tattcaactg attacgtaag
    13441 ccctgcaaat acgcatccag ttgctggctc tctttttccg ccagctctga gcgtaagcgc
    13501 gctaattcct ggcggttatt ggcagacagc tgcgccagct ccagttcatc aacgagcgcc
    13561 ttaagacgtg cagagtcaga ctgcaacgcg aaattttgtg cctgattgag cggagtattg
    13621 ccggtaagcg ttcccaggcg gcgctcgatc tcatttaact gacggcgggc gtcggtttgc
    13681 tgttgcggca gttgattcag cgaatcggca atctcgcggg cgcgctcctg ctcttgctgg
    13741 gcctgacggc ttttatccag caactggctg ctgacctgga gaatttcctg attcagcgcg
    13801 tcggtagaca ttcccggcga cacgctgcgc ggctcgtcac gcatgttgtt taattgtgcg
    13861 cgcagagtag cggagagttt cggataatta tcgataactt gctgatattg tttgatgcgc
    13921 tcaagggaac cttttcgttc ctcaagcgca tttaaggcag actggagcgc ctctacgact
    13981 tccggctgtg cgggtttcgc cgcttttgcc tgctccagtt cctgagtgat ttgtttgcta
    14041 tcgggggccg tcgcggcgta cgccccccaa ctgaggcacc aggccatcag aaaagtgata
    14101 atcaggcgca cgtcagcgtt tcctttgatg gattagacct ggtctttttt atcgtcaacc
    14161 aatggcctgg cgtcgtgttc tgcttcgatc tcttcagcag gaagtggggc gggttcagcg
    14221 tctggcgtaa caaaggtttc ggtagatact gccagcggct ggccaatttt cgtgacagac
    14281 aggctttcca gttgctcaac cagattcact ttacccggtg caaacaggtt gataacggtg
    14341 gaaccgagtt taaagcgacc catttcctgg cctttcagca gtgccacaga accgtcgttt
    14401 tccccggcag gccaggtcca gcgcttgatg ataccttcgc gcggcggcgt aatggtgccc
    14461 gcccagaccg tctcaatgct gccaacaatc gtcgctccga ccagaatctg cgccattggg
    14521 ccaaattcgg tatcgaaaag gcaaatcacg cgttcgttac gggcaaacag attcggcacg
    14581 ttctgagcgg tgagatggtt aacggaaaag agatcgcccg gcacgtagat catctcacgc
    14641 agaataccgt tgcacggcat gtgtacgcga tggtagtcac gcggggagag gtaagtggtc
    14701 acaaacgtac cgttgcggaa caggtccgcc atcagatagt tgcctgccag cagggcttcg
    14761 aggctgtagt tgtggccttt ggcttgcagg attttatctt cttcgatttt acccaactgg
    14821 ctgataacgc catcggcagg catgaccagt acattcggat cggtatcgat tgggcgtact
    14881 tcgtcacgca gcggacggac aaagaattcg ttaaaggtgc ggtagctggc ggtgtccggc
    14941 ttttgcgcct ctttcatgtc gaccttgtag tatttaacga acagatcgat aaccagtttt
    15001 gtcagccatc ctgcccgctt gcttgcgccc caacccgcca ggcgagtaag ccatagtttc
    15061 ggcagaatgt actgtagcga aagtttaaat gaatttaaca aggtagcctc caggccattg
    15121 ttttgtcgtt cctgatccgg ccaacatgcc ggatcctgaa aaaaagggga cgattctaac
    15181 gacggttagc ttaattgtca gccatccgta tcagaaaagt ttttacgcgt ttttacctgc
    15241 gccatgcttt ccagaatacg gtgatagttt tcgaaacggg tttccgcgat tttcccttcc
    15301 tcaaccgctt cccggatagc gcagcccgga tcggtatcgt gtttgcaatc gcgatattta
    15361 cacagaccta aatagtcatg gaattcgaca aagccctgag tgatttgttc cggctccagg
    15421 tgccagaggc cgaactcacg cacaccaggg gaatcaatca catcaccgcc gtgcgggaag
    15481 tgatacagcc gagcggcggt cgtggtgtgc tgaccgagcc ccgagttgtc agagacatcg
    15541 ttggtcagga tctctttttg cagccccagc aacgcgttca gcaggctgga tttaccgacg
    15601 ccagactgcc cggcaaaaat gctgatgcgc ccggtcaacg cctcttccag cggtttcagc
    15661 ccatcctgag tatggctgga aaccatcaat acgcgataac cgatgttgcg gtagatatcc
    15721 atctgctcgt tgacgaacgc catgccttcg tcgtccagca ggtctatctt gttgagcacg
    15781 ataatcggct caatctgcaa ggtttcgcag gccaccaggt aacggtcgat aatattgagc
    15841 gacagctccg gcaaaatggc ggagacaatg acaatctggt cgatgttggc ggctatgggt
    15901 ttcacgccgt cgtagaaatc cggacgcgtt aacaccgagg tgcgctcatg caccgcttcc
    15961 acgatccctt tgatatttac gccttccgcc gccggtttac ccggacgcca gactacgcgg
    16021 tcgccggtta ccagcgaacg gatggtacgg cgaatattgc agcggtgaac gtcgccatcg
    16081 gcggattcca catcagcgtg cataccaaag cggctgatga cgataccttc atcaggctcg
    16141 ccaaacagat tgtcgtcgta gtcgggcttc tccttagacg ttttaagacg acgctggtga
    16201 ttggcgttca cgcggcgctg ctggcctttg gagagtttat ttttactcaa tcgtactgac
    16261 tcctggtcgc ccctgatggg caaaacatct atgatacacg caattgtgga tcaatatagt
    16321 cactgtgaat gggtggaaaa tagcatgagt gccaatgaaa acaacctgat ttggatcgat
    16381 cttgagatga ccggtctgga tcccgagcgc gatcgcatta ttgagattgc cacgctggtg
    16441 accgatgcca atctgaatat tctggcagaa gggccgacca ttgcagtaca tcagtccgat
    16501 gaacagctgg cgctgatgga tgactggaac gtgcgcaccc ataccgccag cgggctggta
    16561 gagcgcgtga aagcgagcac gatgggcgat cgggaagctg aactggcaac gctcgaattc
    16621 ttaaaacagt gggtgcctgc gggaaaatcg ccgatttgcg gtaacagcat cggacaggac
    16681 cgtcgtttcc tgtttaaata catgccggag ctggaagtct acttccacta ccgttatctc
    16741 gatgtcagca ccctgaaaga gctggcgcgc cgctggaagc cggaaattct ggatggtttt
    16801 accaagcagg ggacgcatca ggcgatggat gatatccgtg aatcggtggc ggagctggct
    16861 tactaccgcg agcattttat caagctgtaa aatttttcct ggtcacgtaa gcgccggaat
    16921 ccacttgcgg cggcgtgatt ttgacgctaa atcgaacgtc ttgctgataa tttcagcact
    16981 tgagataaaa acgcaaaaaa aacttttttg gggggttgca gagggaaaga tttctcgtat
    17041 aatgcgcctc ccgtaacgac gcagaaatgc gaaaattacg aaagcaaaat taagtagta
//
LOCUS       DAJVQH010000081        17037 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJVQH010000081 DAJVQH010000000
VERSION     DAJVQH010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.081"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..170
                     /locus_tag="OQM87_002723"
     CDS             <1..170
                     /locus_tag="OQM87_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCS1508797.1"
                     /translation="SRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAV
                     FGGELTLVQLQ"
     gene            complement(171..362)
                     /locus_tag="OQM87_002724"
     CDS             complement(171..362)
                     /locus_tag="OQM87_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCS1508798.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(467..703)
                     /locus_tag="OQM87_002725"
     CDS             complement(467..703)
                     /locus_tag="OQM87_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCS1508799.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDARIMRLA"
     gene            complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="OQM87_002726"
     CDS             complement(821..2260)
                     /gene="rsmF"
                     /locus_tag="OQM87_002726"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCS1508800.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2340..4838)
                     /gene="yebT"
                     /locus_tag="OQM87_002727"
     CDS             complement(2340..4838)
                     /gene="yebT"
                     /locus_tag="OQM87_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCS1508801.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTITIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLMTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKV
                     TGEMTVDPSVVTLLRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVV
                     VPGEKALLQEPDVLTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAI
                     EPQHRELVKGDSKFVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASY
                     PLYANLEKALENSLSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAF
                     DIDLHIKPEYRNLLTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGA
                     SASQRKGDKRILYASETAARAVGRQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLI
                     TARNEVQAKAVLYPEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGN
                     PRRDFELEEATITDSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLS
                     DRVMIAMRISKRYQHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATP
                     PGTPLAPKAQEGKHFLLQESEPKEWREWGTALPK"
     gene            complement(4807..6091)
                     /gene="yebS"
                     /locus_tag="OQM87_002728"
                     /pseudo
     CDS             complement(4807..6091)
                     /gene="yebS"
                     /locus_tag="OQM87_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6221..6718
                     /gene="msrC"
                     /locus_tag="OQM87_002729"
     CDS             6221..6718
                     /gene="msrC"
                     /locus_tag="OQM87_002729"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCS1508802.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6815..7513
                     /gene="proQ"
                     /locus_tag="OQM87_002730"
     CDS             6815..7513
                     /gene="proQ"
                     /locus_tag="OQM87_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCS1508803.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7533..9581
                     /gene="prc"
                     /locus_tag="OQM87_002731"
     CDS             7533..9581
                     /gene="prc"
                     /locus_tag="OQM87_002731"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCS1508804.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9773..10654
                     /gene="htpX"
                     /locus_tag="OQM87_002732"
     CDS             9773..10654
                     /gene="htpX"
                     /locus_tag="OQM87_002732"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCS1508805.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10700..12073)
                     /locus_tag="OQM87_002733"
     CDS             complement(10700..12073)
                     /locus_tag="OQM87_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1508806.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12250..13041
                     /gene="kdgR"
                     /locus_tag="OQM87_002734"
     CDS             12250..13041
                     /gene="kdgR"
                     /locus_tag="OQM87_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCS1508807.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13184..13423)
                     /locus_tag="OQM87_002735"
     CDS             complement(13184..13423)
                     /locus_tag="OQM87_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508808.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13582..13725
                     /gene="mgrB"
                     /locus_tag="OQM87_002736"
     CDS             13582..13725
                     /gene="mgrB"
                     /locus_tag="OQM87_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCS1508809.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13800..14087
                     /locus_tag="OQM87_002737"
     CDS             13800..14087
                     /locus_tag="OQM87_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCS1508810.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14757..14900
                     /locus_tag="OQM87_002738"
     CDS             14757..14900
                     /locus_tag="OQM87_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCS1508811.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14913..15122
                     /gene="cspE"
                     /locus_tag="OQM87_002739"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14913..15122
                     /gene="cspE"
                     /locus_tag="OQM87_002739"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1508812.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15288..16097
                     /gene="rlmA"
                     /locus_tag="OQM87_002740"
     CDS             15288..16097
                     /gene="rlmA"
                     /locus_tag="OQM87_002740"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCS1508813.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16094..16660)
                     /gene="mntP"
                     /locus_tag="OQM87_002741"
     CDS             complement(16094..16660)
                     /gene="mntP"
                     /locus_tag="OQM87_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCS1508814.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4497 a         4127 c         4305 g         4108 t
ORIGIN      
        1 gtagccgctc gcgattaggt gaacgcccaa aagggcaggg aattacaggt gctgatcatt
       61 tctggtttgg tcatacaccg ttgcgacatc gcgtggatat tggcaacctg cattatattg
      121 ataccggtgc tgtctttggg ggcgaactga ctcttgtgca actgcaataa ttaaaaatca
      181 ccatactcct gtgctggtcg ccagaaacca tctataaaat cctcaatcgg aaaacaaccg
      241 ccatggcgga tccgttgatc gctcatagaa taaagacact gctgttccgt gttgtagaca
      301 tccacaacaa tatcttcaca accgccatcc aggtagcaaa caaaaagtac cagcgcgaac
      361 atttcatccc cgaagtgtgg tgccgtaccg ttaagtttag gagagatttt acaacggggg
      421 aataaccagg acaaataacc cgccatgccg acgggttctt tttggatcag gcaagacgca
      481 taatcctggc atcagattgt tgatcgtaac gggtacggta aaggacagag tgttcgccat
      541 ttagcagggc agggatactg gtttcagcat cccaggcatc cagttgcata cataaatcgg
      601 ggtcagattt acaaggaatg gttaacgttc gatcacccgg cgattcaccg tgtaattcgc
      661 cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt catcttcgtg ctctccattt
      721 agctgctgat tgtggtacga ccagtccgat cgcacaattt ttcagcctag ttgaggccgc
      781 cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg tcatgcgtta ctggtaaaaa
      841 gtttcccgtc ccgcaccagt tcacgcgggt agctgttttt cagtcgcgaa ccaatccgtt
      901 tggctaaacc aatcggctga tgctggaaag taaccaacac gtcatccgcc actggcgcgg
      961 cttgcgggta aacatcgcgc ccgcgatacc actcctccgc ttcctgcggt gtcagttcaa
     1021 aagcgtttac attgtcgggg gaggcatggg caataaccgc ttcatgctgc cagcgataac
     1081 ctttgttatg cgtttcggca agtttaatcc ctaagcgaga aaatcggact ttaccgatca
     1141 gggcttcaat gcctaccggg aacaaccaca gttctttgtc gcgttgccag agtcgcaggt
     1201 ttccatccca gtttaagcca acacttgcag ccgcctgacg aatttgtccg gcttcgcgat
     1261 ctttcaccgg gctgaacggg aaattgccca ctttgtattt tggggcgggt aaggcgggga
     1321 tcgcctgagt tttacgcaga cgagcaacga agaagccttc gcagtcgtaa atttgtggga
     1381 aaacatgcaa aaaaccttct tcggtcagcg ccttgtttgc accagggaag agatcgccaa
     1441 gtggtaaaaa ctctacttcg tcggggtaag tctctttcag ccaccggcaa acggcttcgt
     1501 tttcttcccg gtttaaggta caggtcgagt aaaccagcgt accgccagga cgtaatgcat
     1561 gaaaggcgct gtcaatcagt tcccgttggg tagctgcgat ttcctgattg ctttcgggtg
     1621 accagttttt tagcgcatcg ggatctttac gcaccacgcc ttcgccggag cagggagcgt
     1681 ccagcaaaat ggcatcaaac atttctggca ctgacgcacc aaacacgcgg ccaccaaaat
     1741 gtgtgagcgc aacattactg atgccacagc ggctgatatt ggcatgtaac acttttaccc
     1801 gactggcgga aaactcattg gcaaggattg ccccttcgtt attcatccgc gcggcaattt
     1861 gcgtcgtttt tgagcctggc gcagcagcga catccatcac ccgctgtggt gcattaccgt
     1921 cagcaaacaa ggcggcgacg ggcaacattg aactggcttc ctgaatataa aacaggccac
     1981 ttaaatgctc ggtggtacta cccaatggca atgcatcttc attgtcgcgt tcaatccaga
     2041 aaccttcttc acaccacgga attggcgtaa gcgtccagcc ataaggagcg gttaattgca
     2101 ggaaatcagc aacggagatt ttcagcgtat taacgcgaat gctgcggcgc aacgggcgct
     2161 gacaggcggc aagaaaatca tcaaatgaga gcgtcgaagg catagcttcg cgcatttgtg
     2221 tcagaaacgc gtccgggaaa taaacggcgt gttgggccac gagcatgtac cactggcaaa
     2281 aaaacaggcg cgcagtttag cataaacgct ccggcgcagg cacgccggag cagtgggcat
     2341 tatttgggaa gcgcagttcc ccattcacgc cactctttcg gttcactttc ctgcaacagg
     2401 aagtgtttgc cttcctgggc tttcggtgca agtggcgtac ccggaggcgt ggcgaaggcg
     2461 atgccgccac ggataaattg gttaaaggtg ccggttttca ctacgccgcc cgtcagacca
     2521 aagtccagac tgtaacccga tgccaaccag aagacggaat tgttacgcac caggtgttga
     2581 tagcgtttac tgatgcgcat cgcaatcatc acgcgatctg acaatgtccc cagcgtcatt
     2641 ccggtaaccg tacctacttc cagaccacgg aacagcacag gcgtaccgat gcctaacgaa
     2701 ccggcttccg gcgcttcaac aataatgctt aaaccatcta ggtaacgcga atcagtaatg
     2761 gtggcctctt ctaattcaaa gtcgcgacga ggattgccac ggcctggttc gacgttgata
     2821 tacggctgga ggatagtatc tagatgctca acgccagctg ccgagatttg cggtgtgacc
     2881 actgagaagc gcgtaccacc gcgagcaaag gtctggacat attccggata gagcaccgcc
     2941 tttgcctgta cttcattgcg tgcggtaatc agatccagcg tctggatttg cccgatatca
     3001 ataccgagat agcgaattgg catcccgacc gccagttttc cggcatcgaa agcgtgaagc
     3061 gtaatctgcc tgccaaccgc acgggccgct gtttcggaag catacagaat tcgcttgtcg
     3121 cctttacgct gactggcgct ggcaccactg aggttatcga agctaatggc cccctttaat
     3181 gctctggaga gcggggatgc ctgtacggtc aggccactac cattcagctg aactttcgcc
     3241 ccgccttctg cccagaacac gctgttgctg gtcaaaaggt tgcgatattc cggtttaatg
     3301 tgcagatcga tatcgaacgc gttagctcgc ggacgcacgg taataacttc accaacttca
     3361 aatttacggt acagcactac cgatcctgcc tgcacatccg gcagcgtctc tgcactcaaa
     3421 ctcacggttg tggtgggtaa atcgctaagg ctgttctcca gcgctttttc cagattggca
     3481 tacagtggat agctggcttt catctcgcct ttatcgcccg gcagaatacg tatcccgccg
     3541 ttaatccatt ctgaggcgct ggcaccgaga aactcaacgc catccagccc caccttcacg
     3601 tcgacacggc tgttgacgac aaatttgcta tcgcctttta ccagttctcg atgctgaggc
     3661 tcgatggcga cggtaaaggt gacgcctttg ctggtgagtt tacgatcgat aacctggcct
     3721 acctgcacgc cgtgaagaat gagcggctga cccgcatcaa taccgtaact ttccggtgct
     3781 gtcagggtca gtgtcagaac gtccggctct tgcagcaggg ctttttcgcc cggcacaaca
     3841 acgaactctt tgcgtggctc gccatcgccg ggcaccaact caaaggtttt tccggtcagc
     3901 agggcgctga gattagcatc gctaagggat aatttcgggt tgcgtaattc tatgcgggta
     3961 ttttcacgaa gcagggtaac gacgctggga tcaacggtca tttccccagt gactttacca
     4021 ccaggattta aatccagttt agtcagctgt ccgacttcca gcccctgata cattaacggc
     4081 gtcgagtcgg cggttaatcc tgctccactc ggcagttcca gtttcacctt cgcgccactg
     4141 atactgacgt tggcatcaac gccggaaacg ttccagaaac ggctaccttt tttcaccaga
     4201 tcggtaaaac gccgctcgat caggacatca atcaccacgc cttgcttgtt gggattgatg
     4261 gcatagtcgt agacttttcc caccgggatc ttgcggaaat agaccaatga accgctgttc
     4321 agcgaaccga gatcgggggc ttgcaggtgg atcatcagat cgccattgtc cagccgatat
     4381 ttcggttggg tatcgagcgc gacaaagtga tcctgctctt tacctttacc cggcatcatg
     4441 ccgatatagt tcccaccgac gagggcgtcc agcccggaga cacctgccaa cgatgctttt
     4501 ggcgtcatca gccagaactg agtctcttcg cgcagcgcat ctttcatatc ggacttgatg
     4561 ctgaccttga cttcaatctt acgaagatcg tcgctgaggc tgatatcctg cactgttccg
     4621 acttcaacgc cctgataacg aacaggcgta cggcccggaa caataccatc cgccgacata
     4681 aagtcgatgg tgatggtatt accccggtcc tgataactgt cccaaatcag ccaaccggca
     4741 atcattagcg cgatgaaagg cagcagccag aaaggtgaga tacggcgttt atttttaatc
     4801 tgcgcttcag tcgtcgaagc gggcgtttcc tgactcatgt gcatcccaaa gtaagcggct
     4861 gtccagccat tccacagcaa gaatagtcaa aattacggca gcgccaaaat aaaacgcagc
     4921 cggtcccata gtaaaagcga ggatctgatc gcgattaatc agcgacatgg ttaaagatat
     4981 gacaaacagg tcgagcatcg accagcgtcc aatccaggtc accatccgca gtaacagaat
     5041 gcgtgtgcgt aaaccttgct ggcatttaaa atgaatgctc aacagtaaag tgaacatgac
     5101 gatcacttta gtaaacggta ccagaatact ggcgataaac acgattcccg caaccgcaat
     5161 gttgctactt gccagcgaca taattccgga aagaatcgta tcttcctgcc ggccaccatt
     5221 cagataaatg atagaaatag gcaataggtt ggctggtaac aacaaaacga ttgacgctaa
     5281 cagcgccgcc cagcattttt gcaaactatg acgacgacgc aggcgtagcg ggatatggca
     5341 acgcgggcag agaccacgct gatcaggata gccggtaaaa tggcacccaa gacagacacg
     5401 aagtttctcg tccctacgcg tagcggggcg ctgcggataa tatcgctccc acagctcctc
     5461 aacattaaga tgtgacaacg tcaccgtcgt taaaatcacc aacgccacaa aagagaacaa
     5521 gccgacaccc gcctggatat gggcataatc ctgtacctta tatagaagca acgccaatgc
     5581 cgaccaggta gatatccagc attacccact ctttaagtcg ctcaagcatc agcagtaccg
     5641 gacgtaaatt cattcccagt cggttaccaa accataaata tgctatggag gacaccagaa
     5701 tgaggggggc accaataacg cagaaaaaga ccattgatcc cgttatcgca tcgccctgtt
     5761 tggtcatttg ccagatgcct tgcatgacgt tggcgtcaat acgaatgcct aacagccaga
     5821 tatgcaacag cggttcgccc caggcaaacg gcataagcaa cagcatggtg aaggccattg
     5881 ccgccaggcg cgttagcgac cagtcgcgcc cgtcgcgaat ttttgcctga cagcgcggac
     5941 aataggcact ttgatgagaa tttatctcgg gcaggctaaa cagcatgtca cattgcgggc
     6001 aacgttggta atcaccacgc ggcagttcct cgccgattgc cctcactgtt atcttttttg
     6061 tcggcgtaat ttgtggtgtg ttaagagcca tgacgtgcca aatgtaataa gaattaacat
     6121 taatcttaac ccatgatggg aatgatcttg agcattaacg catttaatgc ttattttaat
     6181 aggctggcag ttccaggtaa gacaaccaag tgattaaata atgaacaaaa cagaatttta
     6241 cgcggattta aatcgcgact ttaacgcgct gatggcggga gaaaccagtt ttctggcaac
     6301 gcttgcgaac accagtgcgt tgttatacga gcgtctcact gacgttaact gggcaggttt
     6361 ttatttgctt gaggacgata cactggtact cggaccattt cagggcaaaa ttgcctgtgt
     6421 ccggataccc gtcgggcgcg gggtgtgcgg cacagcggtt gcccgcaatc aagtgcagcg
     6481 tatcgaggat gttcacgcgt ttgacgggca tattgcctgt gatgcggcga gtaattctga
     6541 aattgttctg ccgctggtgg tgaaaaatca gattattggt gttctcgaca tcgatagtac
     6601 cgtcttcgct cgctttacag acgaggacga gcaaggctta cgtcagcttg tggcacagct
     6661 tgaaaaagtg cttgcaacga cggattacaa aaaattcttt gcgagcgtcg caggataatc
     6721 aacggataac gtagcattta ctggtagcgt cattataatg acgcctgttc atgcctgcgc
     6781 ttgttggcta cgtccgttgt aatcaggaaa tttcatggaa aatcaaccta agttgaatag
     6841 cagtaaagaa gtaatcgcgt ttctcgccga acgttttccc cactgtttca gtgcggaagg
     6901 tgaagcgcgt ccgctgaaaa ttggtatttt tcaggatttg gtcgatcgtg ttgccgggga
     6961 aatgaacctg agcaaaacgc aattacgatc cgctttacgt ctctacactt cgagctggcg
     7021 ttatctttac ggtgttaaac ccggcgcaac gcgtgtcgat cttgacggca acccatgcgg
     7081 tgagctggac gagcaacatg tagagcatgc tcgcaagcag cttgaagaag cgaaagcgcg
     7141 tgttcaggca cagcgtgctg aacagcaagc gaaaaaacgc gaagctgccg cagctgctgg
     7201 tgagaaagaa gacgcaccgc gccgcgaacg caagccacgt ccgaccacgc cacgccgcaa
     7261 agaaggcgct gaacgtaaac ctcgtgcgca aaagccggta gagaaagcgc caaaaacagt
     7321 aaaagcacct cgcgaagaac agcacacccc ggtttctgac atttcagctc tgactgtcgg
     7381 acaagccctg aaggtgaaag cgggtcaaaa cgcgatggat gccaccgtat tagaaatcac
     7441 caaagacggc gtccgcgtcc agctgaattc gggtatgtct ttgattgtgc gcgcagaaca
     7501 cctggtgttc tgaaacggag gccgggccag gcatgaacat gttttttagg cttaccgcgt
     7561 tagctggcct gcttgcaata gcaggccaga ccttcgctgt agaagatatc acgcgtgctg
     7621 atcaaattcc ggtattaaag gaagagacgc agcatgcgac ggtaagtgag cgcgtaacgt
     7681 cgcgcttcac ccgttctcat tatcgccagt tcgacctcga tcaggcattt tcggccaaaa
     7741 tctttgaccg ctacctgaat ctgctcgatt acagccacaa tgtgctgctg gcaagcgatg
     7801 ttgaacagtt cgcgaaaaag aaaaccgagt taggcgatga actgcgttca ggcaaactcg
     7861 acgttttcta cgatctctac aatctggcgc aaaagcgccg ttttgaacgt taccagtacg
     7921 ctttgtcggt actggaaaag ccgatggatt tcaccggcaa cgacacttat aaccttgacc
     7981 gcagcaaagc gccctggccg aaaaacgagg ctgagttgaa cgcgcagtgg gacagtaaag
     8041 tcaaattcga cgagttaagc ctgaagctga caggaaaaac ggataaagaa attcgtgaaa
     8101 ccctgacacg ccgctacaaa tttgccattc gtcgtctggc gcaaaccaac agcgaagatg
     8161 ttttctcgct ggcaatgatg gcgtttgcgc gtgaaatcga tccgcatacc aactatcttt
     8221 ccccgcgtaa taccgaacag ttcaacactg aaatgagttt gtcactggaa ggtattggcg
     8281 cagtgctgca aatggatgat gactacaccg ttatcaattc gatggtggca ggtggtccgg
     8341 cagcgaagag taaagctatc agcgttggtg acaaaattgt cggtgttggt caaacaggca
     8401 agccgatggt tgacgtgatt ggctggcgtc ttgatgatgt ggttgcctta attaaagggc
     8461 cgaagggcag taaagttcgt ctggaaattt tacctgctgg taaagggacc aagacccgta
     8521 ctgtaacgtt gacccgtgaa cgtattcgtc tcgaagaccg cgcggttaaa atgtcggtga
     8581 agaccgtcgg taaagagaaa gtcggcgtgc tggatattcc gggcttctat gtgggtttga
     8641 cagacgatgt caaagttcaa ctgcagaaac tggaaaaaca gaatgtcagc agtgtgatca
     8701 tcgacctgcg tagcaatggc ggtggggcgt tgaccgaagc ggtatcgctc tcaggtctgt
     8761 ttattcctgc gggtcccatt gttcaggtcc gcgataacaa cggcaaggtt cgtgaagaca
     8821 gcgataccga cgggcaggtg ttctataaag gcccactggt ggtactggtt gaccgtttca
     8881 gtgcttcggc ttcagaaatc tttgccgcgg caatgcagga ttacggtcgt gcgctggttg
     8941 tgggtgaacc gacgtttggt aaaggcaccg ttcagcaata ccgttcattg aaccgtattt
     9001 acgatcagat gttacgtcct gaatggccag cgctgggttc tgtgcagtac acgatccaga
     9061 aattctatcg cgttaacggc ggcagtacgc aacgtaaagg cgtaacacca gacatcatca
     9121 tgccgacggg taatgaagaa acggaaacgg gtgagaaatt cgaagataac gcgctgccgt
     9181 gggatagcat cgatgccgcg acttatgtga aatcaggaga tttaactgcc tttgaaccgg
     9241 agctgctgaa ggaacataat gcgcgtatcg cgaaagatcc tgagttccag aacatcatga
     9301 aggatatcgc gcgcttcaac gctatgaagg acaagcgcaa tatcgtttct ctgaactacg
     9361 ctgtgcgtga gaaagagaat aatgaagatg atgcgacgcg tctggcgcgt ttgaacgaac
     9421 gctttaaacg cgaaggtaaa ccggagttga agaagctgga tgatctaccg aaagattacc
     9481 aggagccgga tccttatctg gatgagacgg tgaatatcgc actcgatctg gcgaagcttg
     9541 aaaaagccag acccgcggaa caacccgctc ccgtcaagta atatcaatca ggcacaagaa
     9601 attgtgcctg attttttaac agcggcaaga tgccgtaaat cagatgctac aaaatgtaaa
     9661 gttgtgtctt tctggtgact tacgcactat ccagacttga aaatagtcgc gtaacccata
     9721 cgatgtgggt atcgcatatt gcgttttgtt aaactgaggt aaaaagaaaa ttatgatgcg
     9781 aatcgcgctc ttcctgctaa cgaacctggc cgtaatggtc gttttcgggc tggtactgag
     9841 cctgacaggg atacagtcga gcagcgttca ggggctgatg atcatggcct tgctgttcgg
     9901 ttttggtggt tccttcgttt cgcttctgat gtccaaatgg atggcattac gatctgttgg
     9961 cggggaagtg atcgagcaac cgcgtaacga aagggaacgt tggctggtca atactgtagc
    10021 aacccaggct cgtcaggcgg ggatcgctat gccgcaagtg gctatctacc atgcgccgga
    10081 catcaacgct tttgcaaccg gtgcgcgccg tgatgcctct ctggttgctg tcagcaccgg
    10141 tttgctgcag aacatgagcc cggatgaagc cgaggcggta attgctcacg aaatcagcca
    10201 catcgccaat ggtgatatgg tcaccatgac gctgattcag ggcgtggtga acaccttcgt
    10261 tatctttatt tcccgtattc tggcgcagct tgccgcgggt tttatgggcg gaaatcgtga
    10321 tgaaggtgaa gagagcaacg gcaacccgct gatctacttt gcggttgcaa cggttctgga
    10381 actggtgttt ggtattctgg cgagcattat caccatgtgg ttctcgcgtc atcgtgaatt
    10441 ccatgctgat gccggttcgg caaaactggt tggtcgcgag aaaatgattg ccgcgctgca
    10501 gcgcctgaaa accagctatg aaccgcaaga agcaaccagc atgatggctt tctgcattaa
    10561 cggtaagtcg aaatcgctca gtgagttgtt catgactcac ccgccgctgg ataaacgtat
    10621 tgaagctctg cgtacgggtg aatacctgaa gtaatatgaa gtaaatacta aaaagcgcgt
    10681 cctgatcgac gcgctttttt tatgccctgg ctcgtggctg agtgatacgt aaaccactga
    10741 cacaggcagc aatcactgcc agaatagccg cagccatcag cgagacgtgc gtaccattat
    10801 caccaaactg atttagcatc agcgccacca gcgccgcgcc actactctga cccagtagac
    10861 gagccgttcc taacatgcca ctggccccac cgctacgttc gcgcggagcg gaggtaataa
    10921 tggtgtggtt attgggtgac tggaataagc caaatccagc gccacataag atcatcggcc
    10981 agataatatt gatatccgca ggtgacgcgg gcagcagaac cagggaaaaa agccccgcag
    11041 ccatgatgaa caaccctaaa gcacccagca atcctgcatg tacgcgttca atcaaatagc
    11101 ctgccagcgg agccatcacc atcgttgcta acggccacgg tgtcagaagt aaacctgttt
    11161 cgacttcact acgcccgagc acggtttgca ggtaaaaggg cagggaaacc attgccagca
    11221 tttgtgcgca gaaagagcaa acagatgtgc aaatagaaag tgaaaacagc gggatacgca
    11281 gtaaatccac cggtagcagc ggtacgggaa gagaaagctg gcggcgaata aagaaaatac
    11341 caacaacaac cattaccacc agttccgcag caattaacgt cagcgattgc ccctgagcga
    11401 aaccactcaa cgcagtgata agcaggccga aggttaacgc gttcatcacg gcgctgggca
    11461 ggtcgaaacg gggtttactg gcgcgagaac cattgggtgg cagaaaacgc atcgccagaa
    11521 gcagggcgat aatacctaac ggtacgttga ttaaaaataa ccatttccag gatgcgatgg
    11581 agaggattgc tgcagcaatt gtcggcccgg cagcagaaga gacggcaaca ataaacgagt
    11641 ttatgcccat ccctctcccc agaaaacgtt gtggatagat caggcggata agtgcggtat
    11701 taacgctcat caacgctgca ccgccgaaac cttgtatgac acgcgcaagg gtgagcattt
    11761 gcagcgaatc agaaagggcg cagaacagtg aagacaacag aaaaacgacc agaccgcatt
    11821 tataaatacg tcgatagcca aacatatcgc ccagaaacga aaacgagagc agggagatga
    11881 caatggcgat ttgataggcg ttcactaccc agatggaact ggctggtgtg gcatgaaggt
    11941 ccgtggcgat tgttggcagg gcgacgttgg cgattgcgcc gtcaaggaca gccatcgaaa
    12001 taccaatcac aatggttaat atcgcaccgt atcgctgggg caatggcagg ccgtcggcct
    12061 gaacttttgg cataggaatt ttatatcttt ggtgaataat caacaggggt atgattctag
    12121 cactgatatt cacggtggat gtcgcagaat tgtaactaac cggcaaaaga tggattgcga
    12181 ccaccctggt gcgaaattat aataaaaaac agttctgatt tttataaaac actcgcaatg
    12241 aggtgataaa tggctaacgc agatctggat aaacagcctg attctgtatc ttccgtgcta
    12301 aaagtttttg gcattttgca ggcgctgggt gaagagcgcg aaatagggat aaccgagctg
    12361 tcgcagcgcg tcatgatgtc aaaaagcacc gtttatcgct ttttacagac catgaaaacc
    12421 ttaggttatg tggcgcagga aggggagtcg gagaaatatt ccctgaccct gaaattgttt
    12481 gaactgggcg ctcgcgcgtt acaaaacgtc gatttaattc gtagcgcaga tatccagatg
    12541 cgtgagatct cccgcctgac caaagaaact atccacctcg gcgcactgga cgaagacagt
    12601 attgtttaca ttcacaaaat tgactctatg tacaatttgc gcatgtattc acggattggg
    12661 cgtcgtaatc cgctgtacag caccgcgatt ggtaaggtac tgctggcatg gcgcgatcgc
    12721 gatgaagtga agcaaattct tgagggcgtg gagtataaac gcagtaccga gcggaccatc
    12781 accagtacag aagcgttatt acccgttctg gaccaggtgc gcgagcaggg gtatggcgaa
    12841 gataacgaag agcaggaaga agggctgcga tgcattgcgg taccggtatt tgatcgcttt
    12901 ggcgtggtca ttgccggttt gagcatctcc ttcccgacgt tgcgtttctc tgaagagcgt
    12961 ttacaggaat atgtcgcaat gttgcatacc gcagcgcgca aaatttctgc ccaaatgggt
    13021 tatcacgact atccgttctg atgagagtaa gaacctgtcg gaatatcaaa cagacaggtt
    13081 ctttatttag catgagaaaa ataaagttga aggtgcgtta tattaaacgc gcttgctata
    13141 agaatatttt actcatgagt gagaatctgg ttatttatta cccttaacca ttatcgacca
    13201 cgatattgct tttacgtaac agtgggcaat ctgttatccc caagaaacca cttttagtgt
    13261 gcaagtattg taccgtgctg gtgcctctgg ccgtcagata ggtacattgc aaacctaatc
    13321 ctgcggcatt ctctttgctt ccaatcaaaa cgccatatcc gctgagtaat aatcctatcc
    13381 ataccagtgc tatcagcata actgtgcgaa tgatgaatcg cattacaatc tcttctcttt
    13441 ttatgttcgc ttaatcgtag cggcaatatg cgctgaagca agctactcat tccgaaaaag
    13501 cacgaatatc gacatagtta ggcgctgttt aactaacgca tgctagttta atgacataag
    13561 gtaggtgaag cggagattgg agtgaaaaag tttcgatggg tcgttctggt tgtcgtggtg
    13621 ttggcttgct tgctgctttg ggcgcaggta ttcaacatga tgtgcgatca ggatgtacaa
    13681 tttttcagcg gaatttgtgc cattaaccag tttatcccgt ggtgatatca ttttttcagc
    13741 actagatttt cttcctctcc agcagtggta gaatagcgcc tctactcaga ggtggtgaaa
    13801 tgaacgaagt tgtaaattca ggcgtgatga acattgcgtc tttggttgta tcggtggtgg
    13861 ttcttcttat cgggctcatc ttgtggtttt ttatcaatcg tgccagttca cggactaacg
    13921 aacagattga actgcttgag gcgttgctgg atcagcaaaa acgtcaaaat gcactgttac
    13981 gtcgtttgtg cgaagcaaac gaaccagaga aagcagataa aaagaccatt gagagtcaaa
    14041 aatcggttga tgacgaagat attattcgcc tggtcgccga acgataatcc ttcctcaatc
    14101 ttgagtaacc ccgtcgttga agggttactc aattcatcct gattatacgc actgtcatgg
    14161 tgcaactgct tcaaattaaa actctggtct gacttcaaat aattatcata ttatttattt
    14221 gcgtaacggg atatcagaaa aagatatttt cctcattaat ttattaggat gtttacatca
    14281 gatttgtgat taagcgtggt attatttatt agcgaaacgt ttctctcttg atttttttgc
    14341 tcactcatca atttttctta ttttaaattt acaatccctt tggggattga cttctcttta
    14401 gggtaattga tagccgttaa ctgactgttt tatgagaaaa agtgatataa ctttttattc
    14461 attgcatagc agaaaatgtg atattgcacg cactatgtaa taacttctcc ctctggcctg
    14521 gaacaactga acttattgaa ctatgttaga aactacgcca gtttaagtat ctgcctgaac
    14581 tggcaaggtt aagcacaatg atatatcggc gcgtattccg ttgcataagt gtgcaaaaaa
    14641 agtggaagac gtatcgagat ttgtgcgtct gatcgagaca tgtttaaaaa tggcttgcca
    14701 taattaacgt tgtatgtgat aaaagatttc gggttaaacg aggtacagtt ctgtttatgt
    14761 gtggcatttt cagtaaagaa gtcctaggta aacacgttga cgttgaatac cgcttctctg
    14821 ccgagcctta tattggtgcc tcatgcagta atgtgtcagt tttatctatg ttatgcctgc
    14881 gggcgaagaa aacaatctaa ggaatttttc aaatggcaaa gattaaaggt caggttaagt
    14941 ggttcaacga gtctaaaggt tttggcttca ttactccggc tgatggcagc aaagatgtgt
    15001 tcgtacactt ctccgctatc cagggtaatg gcttcaaaac tctggctgaa ggtcagaacg
    15061 ttgagttcga aattcaggac ggccagaaag gtccggcagc tgttaacgta acagctatct
    15121 gatcgaatcc actgatctga agtgtgaata cgcttcaatc tcgctataaa gcctcgtcga
    15181 atgcgaggct ttttactatg ctttatcttc gctcctggcg ttcggatatt tgccagccac
    15241 gtgattcgcg ttacacttgc ggcctttagt atcctgccgg agttgtcatg tcgttttcct
    15301 gtccactttg ccatcagcct ctttcgcgtg aaaaaaacag ctatatctgt ccccaacgac
    15361 atcagtttga tatggcgaaa gaagggtatg tcaatctgct gcccgttcag cataaacggt
    15421 ctcgtgatcc gggcgacagc gcggaaatga tgcaagcacg ccgcgcattc ttagatgccg
    15481 gacattatca gccgctgcgt gatgcaattg tcgcccaact gcgggaacgg cttgatgaaa
    15541 aggccacggc ggtgctggat attggctgtg gtgaagggta ttacacacac gcatttgccg
    15601 atgcgttgcc cgaaatcacc acgtttggcc tggatgtctc gaaggtagcg ataaaagcgg
    15661 cggcgaaacg ctatccgcag gtcactcttt gtgtcgcttc cagccaccgt ttgccgtttt
    15721 ccgataccag tatggacgcc ataatacgta tttacgcgcc gtgtaaagca gaagaattag
    15781 cacgagtagt gaagcccggc ggctgggtca ttactgccac gccgggaccg cgacatttga
    15841 tggagctgaa ggggctgatt tacaatgaag tacatcttca tgcacctcat gcagaacaac
    15901 tggaaggttt tacattacag cagagtgcgg agttgtgtta tccgatgcgt cttcgcggtg
    15961 atgaagccgt cgcattattg cagatgacgc cgtttgcctg gcgtgcgaag ccagaagtct
    16021 ggcaaacact ggcagcaaaa gaagtgttcg actgccagac ggactttaat attcacctct
    16081 ggcagcgttc ttattaaccg tggaagtgcg tccagaggat ctggacgccg atgccgatca
    16141 gcaccagccc gccgagaatt tccgcttttt tcccaataat tgagccgata aagcgaccaa
    16201 ccatcatccc taatgtcgac ataatcaagg ttgcacaacc aatggccaat gcggtcgcga
    16261 taatgttgac ctgaaggaaa gcaagaccaa cacccacagc catggcatcc aggctggtgg
    16321 caatcgcggt ggttaccagt agccagaaac cgtgccgacg gcgcggctct tcatcttcat
    16381 catctgagcc acgaaaaccc tcaataatca ttcgcccgcc gaggaatatc agcagcacaa
    16441 acgcaatcca gtgattccat tcaaggacaa accggctggc taacatgccc attccccagc
    16501 cgatcagcgg cgtcagggtt tcgacggcac caaaaataag gccggttcgc aatgcttcag
    16561 aaaatttcgg tttatggagg ggggcacctt taccgattga tgcagcaaat gcatccatcg
    16621 acataccaaa cgcaagaaga acagtagcag tgatattcat gacaacgtcc tgaccggggt
    16681 atccataaaa cacatcatcg cccccagcaa acatgcatac atgaacaaca agccgcgatg
    16741 ggttttcgcg ttcatgcagg catagcgatt gatgtgcata tggtctcgcc tgactgaaag
    16801 tcttcagtcc gtacgtgcca cgttttgcaa cgagtatgtt gacacgtaca tttccggaat
    16861 ctggaaatcg gctactcccc aaatgagggc gcaaccttaa catattttga gaatataaaa
    16921 caactgttgg aatatattat ttcattgctt tattgataac gattttcatt tatgtttata
    16981 tatcaatgaa acgttaaaat aaataacaaa taagggaaga gataatatag cctaagc
//
LOCUS       DAJVQH010000082        16826 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJVQH010000082 DAJVQH010000000
VERSION     DAJVQH010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.082"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="OQM87_002742"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="OQM87_002742"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCS1508815.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1019..1702
                     /locus_tag="OQM87_002743"
     CDS             1019..1702
                     /locus_tag="OQM87_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCS1508816.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1699..2160
                     /locus_tag="OQM87_002744"
     CDS             1699..2160
                     /locus_tag="OQM87_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCS1508817.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2173..3345
                     /gene="iadA"
                     /locus_tag="OQM87_002745"
     CDS             2173..3345
                     /gene="iadA"
                     /locus_tag="OQM87_002745"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1508818.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTLFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3410..4321
                     /gene="hypT"
                     /locus_tag="OQM87_002746"
                     /gene_synonym="qseD"
     CDS             3410..4321
                     /gene="hypT"
                     /locus_tag="OQM87_002746"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCS1508819.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4314..4706)
                     /gene="iraD"
                     /locus_tag="OQM87_002747"
     CDS             complement(4314..4706)
                     /gene="iraD"
                     /locus_tag="OQM87_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCS1508820.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4703..4786)
                     /gene="idlP"
                     /locus_tag="OQM87_002748"
     CDS             complement(4703..4786)
                     /gene="idlP"
                     /locus_tag="OQM87_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCS1508821.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5379..6209
                     /locus_tag="OQM87_002749"
     CDS             5379..6209
                     /locus_tag="OQM87_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1508822.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(6350..7123)
                     /gene="uxuR"
                     /locus_tag="OQM87_002750"
     CDS             complement(6350..7123)
                     /gene="uxuR"
                     /locus_tag="OQM87_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCS1508823.1"
                     /translation="MKSVTSVQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7338..8798)
                     /locus_tag="OQM87_002751"
     CDS             complement(7338..8798)
                     /locus_tag="OQM87_002751"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCS1508824.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLTLGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8879..10063)
                     /gene="uxuA"
                     /locus_tag="OQM87_002752"
     CDS             complement(8879..10063)
                     /gene="uxuA"
                     /locus_tag="OQM87_002752"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCS1508825.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10403..11746
                     /gene="gntP"
                     /locus_tag="OQM87_002753"
     CDS             10403..11746
                     /gene="gntP"
                     /locus_tag="OQM87_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCS1508826.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11997..12899)
                     /gene="fimH"
                     /locus_tag="OQM87_002754"
     CDS             complement(11997..12899)
                     /gene="fimH"
                     /locus_tag="OQM87_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCS1508827.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(12919..13422)
                     /gene="fimG"
                     /locus_tag="OQM87_002755"
     CDS             complement(12919..13422)
                     /gene="fimG"
                     /locus_tag="OQM87_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCS1508828.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(13435..13954)
                     /gene="fimF"
                     /locus_tag="OQM87_002756"
                     /pseudo
     CDS             complement(13435..13954)
                     /gene="fimF"
                     /locus_tag="OQM87_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
     gene            complement(13964..16600)
                     /gene="fimD"
                     /locus_tag="OQM87_002757"
     CDS             complement(13964..16600)
                     /gene="fimD"
                     /locus_tag="OQM87_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCS1508829.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSIMAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQFYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(16667..>16826)
                     /locus_tag="OQM87_002758"
     CDS             complement(16667..>16826)
                     /locus_tag="OQM87_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone FimC"
                     /protein_id="HCS1508830.1"
                     /translation="ELNAGTRVLENALVPPMGESTVKLPSDAGSNITYRTINDYGALT
                     PKMTGVME"
BASE COUNT         4086 a         4332 c         4095 g         4313 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagcc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaacaatgg ggattgttat
     1021 gacacaacaa ggggatgctg tagccgggga gctggcgaca gaaaaagtgg gcattaaagg
     1081 gtatctggct ttttttctca ccataatctt tttttccggt gttttttccg gaacagacag
     1141 ctggtggcgc gtttttgatt tttccgttct gaacggatcg tttgggcaac tgcctggtgc
     1201 gaatggagcc accacctcat ttcgcggggt gggtggcgcg ggggctaaag atggctttct
     1261 ctttgcgctg gagctggcac cgtcagtcat tctctctctg gggattattt caattaccga
     1321 tggtcttggt ggattacgcg ccgcgcagca gttaatgaca cctgtgctaa aaccgttgtt
     1381 gggcattcca gggatctgct cgctggcact gatcgctaac ctgcaaaaca cggatgccgc
     1441 cgccggtatg accaaagagc tggcgcagga aggtgaaatt accgaacgcg ataaagtgat
     1501 tttcgccgca tatcaaacca gcggtagcgc catcatcacc aactacttct cttccggcgt
     1561 cgcggtgttt gcctttctgg gcacgtccgt aatcgtacct ttagccgtca tcctggtgtt
     1621 taaatttgtc ggagccaaca ttttgcgcgt ctggcttaac ttcgaagaac gtcgcaaccc
     1681 aacgcaagga gcgcaagcat gacaactcag gtccgcaaaa acgtcatgga tatgtttatc
     1741 gatggcgcgc gccgtggctt tactatcgcc acgaccaacc tgctgccaaa cgtggtgatg
     1801 gcgtttgtca tcattcaggc gctgaaaatt accggcctgc tcgactgggt ggggcatatc
     1861 tgtgaacccg taatggcgct gtggggatta cccggtgaag cggcaaccgt ccttttagcc
     1921 gcattaatga gtatgggcgg cgcagtcggt gttgccgcca gtctggcaac cgctgacgca
     1981 ttaaccggcc acgatgtcac cgttttactg cccgcaatgt atttaatggg caacccggtg
     2041 caaaatgtcg gtcgctgcct ggggacagcc gaagtgaatg ccaaatatta cccgcatatc
     2101 attacggtct gcgtgattaa cgcattgctg tcgatctggg ttatgcagct tattgtttaa
     2161 caaggagtta ccatgattga ttataccgca gccggtttta ccctgctgca gggagcgcat
     2221 ttgtatgcgc cggaagatcg gggaatttgc gatgtcctcg tcgctaacgg caaaattatc
     2281 gccgttgcca gcaatatccc ttctgacatt gtaccgaact gcacggttgt cgatctcagc
     2341 gggcagatcc tctgcccagg ttttattgat caacacgtcc atttgattgg cggtggcggc
     2401 gaagcaggtc ccacgacgcg cacgccggaa gtggcgctaa gtcgcctgac ggaagcgggc
     2461 gtcacgtcag tggttggtct gctgggcacc gactctatct ctcgccacct ggaatccctg
     2521 ctcgccaaga cccgtgcgct caatgaagaa ggcatcagcg cctggatgct gaccggcgct
     2581 tatcatgtcc cttcccgcac tattacgggt tccgtggaaa aagacgtggc gattatcgat
     2641 cgtgtgattg gcgtgaaatg cgccatctct gatcaccgtt ctgccgcacc ggacgtttat
     2701 cacctggcta atatggcggc agaatcccgc gttggcggtt tgctcggcgg taaacctggc
     2761 gtcaccctgt tccacatggg cgacagtaaa aaggcgttaa agcctattta tgacctgctg
     2821 gaaaactgcg atgtgccgat cagcaagctg ctgccgaccc acgttaaccg caacgtaccg
     2881 ttgtttgagc aggcgctgga gttcgcgcgc aaaggcggca ccatcgatat caccagcagc
     2941 attgacgaac cggtcgcccc tgccgaaggt attgcccgcg ccgttcaggc gggtattctg
     3001 ctggcacgcg tcaccctcag ctccgacggc aacggtagcc agccgttctt cgatgacgaa
     3061 gggaatttaa cccatatcgg tgttgcaggt tttgaaacgt tgctggaaac cgtgcaggtg
     3121 ctggtcaaag actatgattt cagtatcagc gatgccctgc gcccgctcac cagtagcgta
     3181 gccggtttcc ttaacatgag cgggaaaggc gaaattctgc caggcaatga cgcagactta
     3241 ctggtcatga cgccagaatt gcgcattgag caggtatacg ctcgcggcaa actgatggtc
     3301 aaagacggca aagcctgcgt gaaaggaacg tttgaaacgg cttaaacggt tgtcagccgc
     3361 cctaatggac gtagaatgcc cccaagggcg gctgacagag taaaacgtaa tggatgactg
     3421 tggtgcggtt ttgcataata ttgaaaccaa atggctttat gattttctga ctctggaaaa
     3481 atgccgcaat ttttcccagg cggcagtcag tcgcaacgtc tcgcaaccgg cattcagccg
     3541 ccgcatccgt gcactggaac aggcgattgg cgttgaattg tttaaccgcc aggtgacgcc
     3601 actgcaactc tcagaacaag ggaaaatctt ccattcgcag atccgccatc tgttgcaaca
     3661 gttagaaagc aaccttgcag agctgcgtgg cggcagcgat tacgcgcaac gtaaaatcaa
     3721 gatagccgct gcacactctc tttccctcgg tctgttaccg tccattatca gccagatgcc
     3781 gccgctcttt acttgggcaa ttgaagccat tgatgtcgat gaagcggtcg ataaactgcg
     3841 tgaagggcaa agtgactgta ttttttcctt tcacgacgaa gatttgctgg aagcgccgtt
     3901 tgaccacatt cgcttatttg aatctcaatt gttccctgtc tgcgccagtg acgaacacgg
     3961 agaagcactt tttaacctcg cgcagccaca ctttccgtta ctgaattaca gccgcaactc
     4021 ctacatgggg cgattgatta atcgcaccct gacgcgccac agtgagttaa gtttcagcgc
     4081 cttttttgtc tcttcgatga gcgagctttt aaagcaggtt gccctcgacg gctgtgggat
     4141 tgcctggctg ccggagtatg ccatccaaca agaaattcgc agcgggcagc tcgttgtgct
     4201 gaatcgggac gaacaggtga tcccgattca ggcctacgca taccggatga atacccgcat
     4261 gaatccagtt gccgaacgct tctggcgtga actgcgcgag ctggagattg tgcttagctg
     4321 acattctcaa gcgtcgcact gcgccaaccg ccattatccc agaccagatc aaacgttagc
     4381 ggttcatcgt cgtggtacag cattatctca aggctgatcg ccagagtgtg gcagtacgct
     4441 tctttcaaat taacctgcaa cgccgcaatc cgtggctcat gaaataacag tgcagaacgc
     4501 agctgacggc aataccagct ctggagatgt ttgtcgctcg ggttatgcac ttgccactcg
     4561 ggcatgcctg aaacaagtaa tgacggtaat gccgaatgag gagaattaaa aagagtaagg
     4621 agatcgctgc ataccgattt acgcagcaat gacgttttat tgcctgaaat ttcaggtaaa
     4681 acagcctgaa gtgattggcg catcattttg cgcactcctg acgtttagca acatatgcgg
     4741 cttgccctga acaccgggca ccactgtatt gatgctcatt ttccatagcc attccctttt
     4801 gttcattaca tccatgtcag tctgagacag gcctgaatcc tgtcagcaaa gtataaaacc
     4861 ttacttgcct atgaatatct actgactttt caggaaaaaa gtcatgattt gataaaaatc
     4921 atcaaactca aaaaaaaacc gctagtatat tctgaatatt ccgaatagtt acgtagatga
     4981 aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc cgcataatca
     5041 gccatgtatt atttgaaaca attagtttgt aaataatata aattgataac atatcaaata
     5101 catcaaaaat accattcaca ggcattatta atatagacct caatatagca acatcaaatt
     5161 ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat gagcgttttt
     5221 tgatacttta tagtgtttcc taccctttaa tatcactgtg tagagttgat atcactacaa
     5281 cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt tatctatttt
     5341 tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca ttaagcaatg
     5401 ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa gtcgacagtg
     5461 agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat cttctgcctc
     5521 ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt agaaaactta
     5581 atattggaaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga gaattcaatg
     5641 gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca gctttagacg
     5701 aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca gaaggtttac
     5761 ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca gttcatgaaa
     5821 taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata ggatatattt
     5881 caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga attgatatct
     5941 atattgatca gataataact gtctcagggg tagcaaattc ttccggattc gcccttgccg
     6001 cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata gaagcctatc
     6061 ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt gatgaagatg
     6121 cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt gaattaaaaa
     6181 atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact aatattcagg
     6241 ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt gcgacttctt
     6301 aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt caggcgtcga
     6361 ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg tcaacgttcg
     6421 agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc agcttcgccg
     6481 ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc aaccactctt
     6541 tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga ttgttttccc
     6601 gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt gcttcagcaa
     6661 tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg gaagccagtt
     6721 cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg gtagcctgca
     6781 gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac agctcaaaag
     6841 gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg cctgagctat
     6901 caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc tccagcatga
     6961 tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt tcacgctccg
     7021 gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg atcatcgccc
     7081 cgacttcctg gtaaggtctt tgcacagagg tgacagattt catggtgttg tgtccgttag
     7141 gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc ctacaacggg
     7201 caattttacg gcccccgtta atctcaaact ataccattag cactggaagt tggcacgtac
     7261 aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac cagtaagtct
     7321 gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg ttcgcacagc
     7381 tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag atcatcggca
     7441 aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata ctgcgcgtta
     7501 atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc ctgctcatcc
     7561 acgccctggg tgtaacgcat ccagccagcc acgcccagcg tcaggtgacg ccagctgccg
     7621 ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt ctggctgccg
     7681 tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg ctcgatcagc
     7741 agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg cgcttgttcc
     7801 tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt gtcggcaatg
     7861 gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc gttcagcata
     7921 cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgcgcc cactttatcc
     7981 cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa cggttcgcag
     8041 gcaatggcgc atgggtcgta aacacccagc tggtcagcaa tttcctgtaa ggtttctggc
     8101 gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt ctcttcaatc
     8161 catgccgcta gttgcgggtc acgcgcctga gccagcccca gtaccgcgac cttcgccaca
     8221 tgaccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag ccctttttca
     8281 cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc agtcgggttt
     8341 tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc cgcatcagcg
     8401 cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg cgccatcgcg
     8461 ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat cgaaccgata
     8521 attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag ttgctgtttt
     8581 ttcaggtttt cgatcagcat acggtcgttg cctggcatca ggttaacttc gcagatgccc
     8641 cagtcgctgt cggtgctttc cagcagatga tgggtataca acgcctggtg cgcgcggtga
     8701 aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc ccatgacggg
     8761 cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag aatccgtctt
     8821 cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc ggtgggtatt
     8881 aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg gccaggcctt
     8941 tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg tccagcatct
     9001 gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct ttacgacggt
     9061 gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg ttcaggtgcg
     9121 ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga tgggtgaagt
     9181 aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca cgcacgccgt
     9241 aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca accatccact
     9301 gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc ggcggatcgt
     9361 ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc gctttcagga
     9421 agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc tccaggtgct
     9481 tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca atgatgttac
     9541 gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct gcctgagcaa
     9601 tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc atttcgaatg
     9661 cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc acgtattcga
     9721 ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg cgaatgccac
     9781 actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca tagttgccag
     9841 tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac accaggcctg
     9901 cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact tcgccgttcg
     9961 ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca tcagctaaag
    10021 aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt cctctttcac
    10081 atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta tccggatgaa
    10141 caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt ctaccttttg
    10201 gtaagacatg tcaacccgaa ctctgaaatt ggttaaccac atcacaagaa tttcactctc
    10261 atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca gaggatgtga
    10321 cattcatcgc aacaatggtt gaccaatttg catgacatgt cgcccaaaat aacaacggtt
    10381 caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg tattcggcat
    10441 tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg ctttgttggt
    10501 ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc tgcacaccat
    10561 gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt tcggtgcggt
    10621 catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata cgctgctggc
    10681 gcgtctaggt ctgcgctatg ttcagctgtc ggtgattatc atcggcctta ttttcggtct
    10741 ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg ttattgccgc
    10801 cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg ccactaccgc
    10861 acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt atggcgcgga
    10921 tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa tctgcgcagg
    10981 tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat tcctgaaagc
    11041 agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc tggtgccgct
    11101 gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg taaaagatac
    11161 ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt ttattgcgat
    11221 ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt gggtgatgaa
    11281 cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg gtgcgggtgg
    11341 cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca tgttgatgtc
    11401 ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa ttcgtctggc
    11461 gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg ctgcaatcct
    11521 tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac tggcgacggc
    11581 tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt tcaaaggtta
    11641 ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc tggtcaactc
    11701 cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga acaccagagc
    11761 ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg acagtcccct
    11821 cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc gctttgttta
    11881 tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt gcaggttttg
    11941 taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta atttttttat
    12001 tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac ctgccctccg
    12061 gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac tgctcctaac
    12121 gataccgtgt tattcgttgg aataatcgta ccgttgcgcg tcaactgtac gccgacgccc
    12181 tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc tgcggttgtg
    12241 ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag aggaattggc
    12301 actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac atcacaaccg
    12361 ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg aaaatcatcg
    12421 ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc agctttaatc
    12481 gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca cggcttatcc
    12541 gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg atagctactg
    12601 ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc cgagcctcgt
    12661 tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa gatttgcgtc
    12721 gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt tacataaaca
    12781 ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca ggcgaatgac
    12841 caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc tcgtttcatt
    12901 acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg cctgaatggt
    12961 tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga agtgcgctga
    13021 ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg tgttgccact
    13081 gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat aatatccggt
    13141 gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg gacaattagt
    13201 caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa gactgaaaga
    13261 ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg gtttggcgac
    13321 gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac ttgcgagcgc
    13381 caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct ccagttactg
    13441 atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag gcacctgtgt
    13501 cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca gggtcgtcca
    13561 cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat tcagaagctg
    13621 tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt tggcattgtg
    13681 gctatctgca acgccggtaa acccaacttt tacggcagaa acagggtgac agcaaaatac
    13741 gaaatggaac gacaggagtc gtcgcgccaa tgttgttaaa ttgcttcgcc gcgttttcca
    13801 tcagatcaac agtaaaattg gttgattcag cggccacact acagccgtta tctctgacat
    13861 agccgcggat agtaatcgtg ctatccgcag ccaaaacgcg acttaccgcc agccacaaaa
    13921 aagcgcacag aagataaaaa ggtttgtttc tcatcacgcc cccttaacga cattcagctg
    13981 atagctgggt taataactgc tgctgactct ctggtggcag ttgataattg gcgacacagt
    14041 gagcattttc ctcttctccc catttcacct gaacttttcc cgctaaaggc attccgctga
    14101 ggtaaaactg accattatcc gcaacaatgc cgctactctg gctactctct gatgtcacca
    14161 tcgccccaaa cggcagcggc ttattattgt gagttagcgt catgagcagt tttatcccaa
    14221 cgcgcgcttt aaactctgct cgcacgatcg ccccacgagt gggaacaacg ttagcgaccg
    14281 cgttatctaa atcgacgtta tcagccaagg tattggtatc cagcgccact ctattttccc
    14341 gatattcagt ggcataaggc agcacggcat aaccacgcca gtcggtacgc acccccgtct
    14401 ggttttcgac ttttgcatct tttgcgccag gcgctttaac aagcaccacc gtatcgttta
    14461 acggctgccc cagcgttacg ccattggcat gagccaatac cccaccgctg actccgtaat
    14521 agagctgctt aatatcatcg ctatggctgt aaccgatatt ggcattgccg taaccaccgc
    14581 gataattcag cgtggcgtag cctgtacttc cgctattacc atcgcctccc ccggcatagc
    14641 cggtttgcac gctataactg aggttgttgt cttccagcaa cgtaccgtat acaccagcca
    14701 gattggtcat ccgaccgttg agatcgtgtg acatgctgta gctggcactg gcatgtcgcc
    14761 actgagattt actgtcagaa cgcagccagt ggctgaaagg aatattgacg ttaagcgcta
    14821 acatctgatc ccgtcctttt tgccaggcgt ttttcgtcag gctatagctg agcgtccagt
    14881 tgatatcttc gaacgcagta tttaatccag cctggaattg ctcatcgaca ttactcgttc
    14941 cccaataagt ttgatggcta ccactcaaat acagtgttga tgtgcgcccg agttgctgag
    15001 taacggtgag ctgtaatttc ccgcgtttgt tataagcgag gttgtaatag tcggtgaatt
    15061 tcggcttaac ctgaataact ccatcctgtg tttcgatgtt gtagccattc attcgactgt
    15121 atgttgtatc agcgaaatta aaatatccgc tggtcgaata acggtaaccc actaactgaa
    15181 tattcgtgcc tgattcattg agcgatttgt tatagagaaa acgcaccgat tgtccgtcat
    15241 gctgactgtc atcgggaagt gtggaattag cctgcgtcat atccacagac agagcgccca
    15301 gtgcctccat gtttttcccg ataccgaaat taaaagcacg ataacgatcc gccagttgcg
    15361 ttccaccata tattgtccag ccagccggaa ggccgtggag taatgtactc tggaaaaagc
    15421 ggggtttttc ctgctgcgca tttccactac ggtattctcc tgccataatg gaataacgag
    15481 tatgcccttc acgttgcaaa agcgggactg acgaataggg tacggtaaaa atctgcgtgc
    15541 tgccgtcagc ctctttgatc gttacctgca agtcaccact attacctgcg gcatagatat
    15601 cgttgatggt aaaaggcccc ggtggcaccg tactattata aatgccatat ccattttgtt
    15661 taatagtgac ctgtgcagta ccatgagcaa taccgtggat caccggggca aatcctcttt
    15721 ggctatcggg taacatattg tcatctgagg ccaattgtgc gccgcgaaag ttaataccat
    15781 cgaaaatatc gccctgggta taaccatcac ccaacgtcag ccgggaacgt aaaggaatta
    15841 tgtctcgctc aatccaggta atgatatgct gccatttatt tttgctacct gatgaactgt
    15901 cgctactgtt ataactccag gtggtattgt cgcgtaaacg ccacgcacca atatttaacc
    15961 cactctgtag gtttaaatat gcataatggc tgttaccccc aatccgattc tgtacactat
    16021 ttccgctgaa attataattg agcaatccgg cattaatacc gggatcccat aactcaggag
    16081 gaatataacc acgcgcgcga ttactcataa atgcctgagg gatcgtcagg ttcagtcgct
    16141 gctgaccaac atccagatgc gcagtagcgt cctggaccat tgtggttaat ggcacacagg
    16201 catcatccgc cagcagattc ataccggcga cagaagccgt attcagcccc atactggcga
    16261 gttgcgcgcg tgtcaggcag ggaacaatcc cttgttcact gtcgcccgta ttaaatgtga
    16321 catcacgcgt tgccatataa ccattattca aatagatatc gacgcgatac gtccctggcg
    16381 gtaattcttg cccattttca aaacgcgata aatcggccac agcctgggga tcatccgcta
    16441 aaaagcgcag attaaaatag aggtcggcag atgacaaagg tgcctgtgcg gcaaaagcac
    16501 aggcgacaac gagtcggaca aaaaaaccag ccaaacgatg cttacgaata tgcaagcatt
    16561 gtgtgtttcg ctggtaaagt cttaaattca gatatgacat tattcctccg gtttaaaatc
    16621 acccggcagt caattctttt tattcaggcg aaaaattccc cctgcgttat tccattacgc
    16681 ccgtcatttt gggggtaagt gcgccataat catttattgt tcggtaagta atattgcttc
    16741 ctgcatcaga aggcaattta accgtgcttt cgcccattgg aggcaccaat gcattttcaa
    16801 gaacccgggt tccggcattc aactct
//
LOCUS       DAJVQH010000083        16680 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJVQH010000083 DAJVQH010000000
VERSION     DAJVQH010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.083"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..2088
                     /gene="pbpC"
                     /locus_tag="OQM87_002759"
                     /pseudo
     CDS             1..2088
                     /gene="pbpC"
                     /locus_tag="OQM87_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2238..2669
                     /gene="ndk"
                     /locus_tag="OQM87_002760"
     CDS             2238..2669
                     /gene="ndk"
                     /locus_tag="OQM87_002760"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCS1508831.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2819..3973
                     /gene="trmG/rlmN"
                     /locus_tag="OQM87_002761"
     CDS             2819..3973
                     /gene="trmG/rlmN"
                     /locus_tag="OQM87_002761"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCS1508832.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4258..5271
                     /gene="rodZ"
                     /locus_tag="OQM87_002762"
     CDS             4258..5271
                     /gene="rodZ"
                     /locus_tag="OQM87_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCS1508833.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5298..6416
                     /gene="ispG"
                     /locus_tag="OQM87_002763"
                     /gene_synonym="gcpE"
     CDS             5298..6416
                     /gene="ispG"
                     /locus_tag="OQM87_002763"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCS1508834.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6527..7801
                     /gene="hisS"
                     /locus_tag="OQM87_002764"
     CDS             6527..7801
                     /gene="hisS"
                     /locus_tag="OQM87_002764"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCS1508835.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7819..8439
                     /gene="yfgM"
                     /locus_tag="OQM87_002765"
     CDS             7819..8439
                     /gene="yfgM"
                     /locus_tag="OQM87_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCS1508836.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8450..9628
                     /gene="bamB"
                     /locus_tag="OQM87_002766"
     CDS             8450..9628
                     /gene="bamB"
                     /locus_tag="OQM87_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCS1508837.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9746..11218
                     /gene="der"
                     /locus_tag="OQM87_002767"
     CDS             9746..11218
                     /gene="der"
                     /locus_tag="OQM87_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCS1508838.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11288..11503
                     /locus_tag="OQM87_002768"
     CDS             11288..11503
                     /locus_tag="OQM87_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCS1508839.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11500..12870)
                     /gene="xseA"
                     /locus_tag="OQM87_002769"
     CDS             complement(11500..12870)
                     /gene="xseA"
                     /locus_tag="OQM87_002769"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCS1508840.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13032..14498
                     /gene="guaB"
                     /locus_tag="OQM87_002770"
     CDS             13032..14498
                     /gene="guaB"
                     /locus_tag="OQM87_002770"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCS1508841.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14567..16144
                     /gene="guaA"
                     /locus_tag="OQM87_002771"
     CDS             14567..16144
                     /gene="guaA"
                     /locus_tag="OQM87_002771"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCS1508842.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16239..>16680)
                     /locus_tag="OQM87_002772"
     CDS             complement(16239..>16680)
                     /locus_tag="OQM87_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCS1508843.1"
                     /translation="QQGKNEEQRQHDEWVAERNREIQQEKQRRANAQAAANKRAATAA
                     ANKKARQDKLDAEATADKKRDQSYEDELRSLEIQKQKLALAKEEARVKRENEFIDQEL
                     KHKAAQTDVVQSEADANRNMTEGGRDLMKSVGKAEENKSDSWFN"
BASE COUNT         3845 a         4168 c         4669 g         3998 t
ORIGIN      
        1 ttaccttgaa gcgctgatca attatgaaga tcgctggttc tggaagcatc cgggggttaa
       61 tccattctcg gtggcgcgcg cagcatggca agatctcact tcgggacggg ttatttccgg
      121 tggcagcacg ctcactatgc aggttgctcg tctgcttgat cctcacccca aaacatttgg
      181 tggcaaaatt ctccagctct ggcgcgcgtt gcaactggag tggcatttgt ctaagcgtga
      241 aattctgatc ttgtatctta accgcgctcc gtttggcgtt acgttgcagg ggatcggtgc
      301 ggcaagttgg gcttatctcg gaaaatcgcc tgcgaattta agctattccg aggcggcaat
      361 gctggcggtg ttaccgcaag tgcccagccg actgcgcccg gatcgttggc cggagcgtgc
      421 cgaagccgcg cgtaataaag tgctcgaacg gatggctgcg caaggtgtgt ggtcccgtga
      481 gcaggtaaaa gagtcaaggg aagaacccat ctggctggca ccccgacaaa tgccgcaact
      541 ggcaccgctg ttttcgcgca tgatgctcgg taaaagcaaa agcgacaaaa tcgttactac
      601 gctggatgcc ggtcttcaac gacgtctgga agaactggcg caaactggaa agggcggttg
      661 ccaccgcgca gctcactggc gatgatcgtg gttaatcata ccgatatgcg tgttcgcggc
      721 tgggtgggat cggttgatct caacgatgat tcacgctttg gtcatgttga tatggtcaat
      781 gcgatccgat cgccaggatc ggtgctcaaa ccgtttgttt atggtctggc gctggatgaa
      841 ggcttgatcc acccggcatc actgctgcaa gacgtccccc ggcgcaccgg tgattatcga
      901 ccaggtaact ttgatagcgg ttttcatggc ccgatcaaca tgagcgaggc gctggtgcgc
      961 tcgctgaact tacctgctgt gcaggtgctg gaagcctatg gaccgaaacg gtttgcggca
     1021 aagttacgca atgttggatt gccgttatat ttgcccaacg gcgctgcgcc gaatctttca
     1081 ctcattctcg gcggcgctgg tgcaaaactg gaagatatgg cggcagcgta taccgcgttt
     1141 gctcgccacg gcaaggcagg caaattgcgc ttacagcctg acgatccgct gcttgaacgg
     1201 cctttaatgt cgtcgggggc ggcgtggatc attcgccgga ttatggctga tgaagcgcaa
     1261 cccttgccgg atagtgcctt gccgcgcgtc gccccactgg catggaaaac gggcaccagc
     1321 tatggctatc gtgacgcctg ggcgattggg gttaacgctc gctatgtcat tgggatctgg
     1381 actggcagac ctgacggcac gcccgttgtt ggtcagtttg gctttgccag tgccgtacca
     1441 ttgttgaatc aggtcaataa tatcttactg tcgcgcagtg cgaatctgcc ggaagacccg
     1501 cgcccgaact cagtcactcg tggcgttatc tgctggccgg gcggacagtc tttgccggaa
     1561 ggtgacggta actgtcgccg ccgcctggca acctggctgc tggatggcag ccagccgcca
     1621 acgctgttac tgtcagagca ggaagggatt aatggcattc gcttccccat ctggctggat
     1681 gacaaagggc agcgcgtcgc cgctgactgc ccgcaggcga agcaagaaat ggtaaatgtc
     1741 tggccgctac cgctggaacc ctggctgccc gcatcagaac gtcgcgctgt gcgcttacca
     1801 ccggcctcga caatttgccc gccatacggt cacgatgccc agctccctct gcagctgaca
     1861 ggcgttcgcg atggcgcgat tattaaacgt ttaccgggcg cagcggaagc gactttgccg
     1921 ttgcaatcaa gtggaggggc aggtgaacgc tggtggtttc tgaatggcga accgttaact
     1981 gaacgcgggc gcaacgtgac cctgcatttg acggataaag gtgattatca attgctggtg
     2041 atggatgatg tggggcaaat cgcgacagtg aaatttgtca tgcaatagtc tgttttgtcc
     2101 ctgattgttg ctaaaactca ttttatttta aaaaaaatgt taccttcatc aatagtcaac
     2161 ggccctgttg ctcattataa tccgcgccat ctcgtacgct ggtacagaca acaacagaac
     2221 aatttacaga ggtaaaaatg gctattgaac gtactttttc catcatcaaa ccgaacgcgg
     2281 tagcaaaaaa cgtcattggt aatatctttg cgcgctttga agctgcaggg ttcaaaattg
     2341 tcggcaccaa aatgctgcac ctgaccgttg aacaggcacg tggcttttat gctgaacacg
     2401 atggaaaacc gttctttgat ggtctggttg aattcatgac ctctggcccg atcgtggttt
     2461 ccgtgctgga aggtgaaaac gccgttcagc gtcaccgcga tctgctgggc gcgaccaatc
     2521 cggcaaacgc actggctggt actctgcgcg ctgattacgc tgacagcctg accgaaaacg
     2581 gtacccacgg ttctgattcc gtcgaatctg ccgctcgcga aatcgcttat ttcttcggcg
     2641 aaggcgaagt gtgcccgcgc acccgttaat aatttcgtaa atgccgcgtg caaacgtggc
     2701 atccgtgcgc cagaatttgt acaatgcagc gcccccggac gagcagccgc tcaccggggc
     2761 gtttcttttt tcaaccctcc aggggccata acgtgtaata acgaggccgg aatagcatat
     2821 gtctgaacaa ttagtcacac ctgaaaacgt caccacgaaa gatggaaaaa tcaacctgct
     2881 ggatctcaac cgtcagcaga tgcgggagtt ttttaaagat ttaggtgaaa aaaccttccg
     2941 cgccgatcag gtgatgaagt ggatgtatca ctattgctgc gacaactttg atgagatgac
     3001 cgacatcaac aaagtgttgc gcggcaaact gaaagaggtg gcggaaatcc gcgcaccgga
     3061 agtggttgaa gaacagcgtt catccgacgg caccattaaa tgggcgatcg ccgttggcga
     3121 tcagcgcgtc gaaacggtgt atatcccgga agacgaccgt gccacgctct gcgtctcttc
     3181 gcaggtgggg tgtgcgctgg agtgtaaatt ctgttccacc gcccagcagg gctttaaccg
     3241 caacctgcgg gtatcggaaa ttattggcca ggtgtggcgt gcggcgaaaa tcgtcggcgc
     3301 agcaaaagtc accggtcagc gcccaatcac taacgtcgtg atgatgggca tgggcgagcc
     3361 gctgctcaac ctgaacaacg tcgttccggc gatggaaatc atgcttgatg atttcggttt
     3421 tggcctgtct aaacgtcgcg tgacgctttc cacttccggt gtagttccgg cgctggataa
     3481 actgggcgat atgatcgacg ttgcactggc aatttccctg cacgcgccga acgacgaaat
     3541 tcgtgacgaa attgtgccga tcaacaaaaa gtacaatatc gaaacgttcc ttgctgcggt
     3601 gcgccgttat ctggagaaat ccaacgccaa tcagggccga gtcactattg aatacgtgat
     3661 gcttgaccac gttaacgacg gcactgaaca cgcgcaccaa ctggcggaac tgctgaaaga
     3721 tacgccgtgt aagatcaacc tgatcccgtg gaacccgttc ccgggcgcgc cgtatggacg
     3781 cagttcgaac agccgtatcg accgtttctc aaaagtgctg atgagctacg gtttcaccac
     3841 tattgtgcgt aaaacgcgtg gtgatgatat cgatgctgcc tgtggtcagt tggcgggcga
     3901 tgttatcgac cgtacgaaac gtactctgcg taaacggatg cagggtgaag ccatcgatat
     3961 taaagcggtc tgatagccat tacgccacgg ttactttatg taccgtggcg taatctttta
     4021 tgaatgaagt attaagttgc aattgtctgg tcaatattaa cggtgcgttt ttgctgactt
     4081 taaggcagta tgtaacgatg caacagtaag ttagcctcag tgaatgtggg ctttgtcacg
     4141 agcacacaga cggtcttata ctgtatgata acggttaact taacggatgt ttcgcggtgt
     4201 gggtgagcat gatggttcac cggcatctca attctcattt aaacgtacct gcagcgaatg
     4261 aatactgaag ccacgcacga ccaaaatgaa gcacttacta ccggcgctcg cctgcgtaat
     4321 gctcgcgaac aactaggact tagtcagcag gccgttgccg agcgactttg cctgaaggtt
     4381 tccacggtac gcgacattga agaagataag gcacccgccg atcttgcttc aacattcctg
     4441 cgcggatata tccgctctta tgcgcgtctg gtacatattc cagaagaaga actgctgcca
     4501 gggctggaaa agcaggctcc acttcgggct gcaaaagttg cgccaatgca gagtttttcc
     4561 ctcggtaaac gccgcaaaaa acgcgacggc tggctgatga ccttcacctg gctggtgttg
     4621 tttgtggtta tcggcctgag cggtgcctgg tggtggcaag accacaaagc tcagcaggaa
     4681 gagatcacca ctatggccga tcaatcttcg gcggaactga gcagtaatag cgagcagggg
     4741 cagagtgttc cgttaaatac gtcgacaact acagacccgg ctacaacgtc cacgccgcca
     4801 gcgtctgtgg atactaccgc aaccaacacg caaacacctg ccgtaactgc gccagcacca
     4861 gctgttgatc cgcaacagaa tgcggttgtt tcgccttcgc aggcaaatgt tgataccgcc
     4921 gcgaccccgg taccaacggc agcaacaacg ccagatggtg ctgcgccgtt gccaaccgat
     4981 caggctggcg tgaccacgcc ggcggctgat ccgaatgcgc tggtgatgaa ctttactgcc
     5041 gattgctggc tggaggtcac tgatgctacc ggtaaaaaat tgtttagcgg gatgcagcgt
     5101 aaagacggta atttgaattt aaccggccag gcaccgtata aactgaaaat tggtgcgcca
     5161 gccgcagtac agatccagta tcaagggaaa cctgtcgatc tgagtcgttt tatcagaact
     5221 aaccaggttg cgcgtctgac cctcaatgcc gaacaatcac cggcgcagta acagacgggt
     5281 aacgcgggag atttttcatg cataaccagg ctccaattca acgtagaaaa tcaacacgta
     5341 tttacgttgg gaatgtgccg attggcgatg gtgctcccat cgccgtacag tccatgacca
     5401 atacgcgtac gacagatgtc gaagcaacgg tcaatcaaat caaggcgctg gaacgcgttg
     5461 gcgctgatat cgtccgtgtc tccgtaccga cgatggacgc ggcagaagcg ttcaaactca
     5521 tcaaacagcg ggttaacgtg ccgctggtgg ctgacatcca cttcgactat cgcattgcgc
     5581 tgaaagtagc ggaatacggc gtcgattgtc tgcgtattaa ccctggtaat atcggtaatg
     5641 aagagcgtat tcgcatggtg gttgactgtg cgcgcgataa aaacattccg atccgtattg
     5701 gcgttaacgc cggatcgctg gaaaaagatc tgcaagaaaa gtatggcgaa ccgacgccgc
     5761 aggcgttgct ggaatccgcc atgcgtcatg ttgatcatct cgatcgcctg aacttcgatc
     5821 agttcaaagt cagcgtgaaa gcgtctgacg tcttcctcgc tgttgagtct tatcgtttgc
     5881 tggcaaaaca gatcgatcag ccgctgcatc tggggatcac cgaagccggt ggtgcacgca
     5941 gcggggcagt aaaatccgcc attggtttag gtctgctgct gtcggaaggc atcggcgaca
     6001 cgctgcgcgt atcgctggcg gccgatccgg tcgaagagat caaagtcggt ttcgatattt
     6061 tgaaatcgct gcgtatccgt tcgcgaggga ttaacttcat cgcctgcccg acctgttcgc
     6121 gtcaggaatt tgatgttatc ggtacggtta acgcgctgga gcaacgcctg gaagatatca
     6181 tcactccgat ggacgtttcg attatcggct gcgtggtgaa tggcccaggt gaggcgctgg
     6241 tttctacact cggcgtcacc ggcggcaaca agaaaagcgg cctctatgaa gatggcgtgc
     6301 gcaaagaccg tctggacaac aacgatatga tcgaccagct ggaagcacgc attcgtgcga
     6361 aagccagtca gctggacgaa gcgcgtcgaa ttgacgttca gcaggttgaa aaataataac
     6421 gtgatgggaa gcgcctcgct tcccgtgtat gattgaaccc gcatggctcc cgaaacattg
     6481 agggaagcgt tgagggttca tttttatatt cagaaagaga ataaacgtgg caaaaaacat
     6541 tcaagccatt cgcggcatga acgattacct gcctggcgaa acggccatct ggcagcgcat
     6601 tgaaggcaca ctgaaaaacg tgctcggcag ctacggttac agtgaaatcc gcttgccgat
     6661 tgtagagcag accccgctat tcaaacgtgc gattggtgaa gtcaccgacg tggttgaaaa
     6721 agagatgtac acctttgagg atcgcaatgg cgacagcctg actctgcgcc ctgaagggac
     6781 ggcgggctgt gtacgcgccg gcatcgagca tggtcttctg tacaatcagg aacagcgtct
     6841 gtggtatatc gggccgatgt tccgtcacga gcgtccgcag aaagggcgtt atcgtcagtt
     6901 ccatcagttg ggctgcgaag ttttcggtct gcaaggtccg gatatcgacg ctgaactgat
     6961 tatgctcact gcccgctggt ggcgtgcgct gggtatcttc gaacacgtaa ctcttgagct
     7021 gaattctatc ggttcgctgg aagcacgcgc caattaccgc gatgcgctgg tggcattcct
     7081 tgagcagcat aaagaaaagc tggacgaaga ctgcaaacgc cgcatgtaca ctaacccgct
     7141 gcgcgtgctg gattcaaaaa atccggaagt gcaggcgctt ctcaacgacg ctccggcatt
     7201 aggtgattat ctggacgagg aatctcgtga gcattttgcc ggtctgtgca aactgctgga
     7261 gagcgcgggg atcgcttaca ccgtaaacca gcgtctggtg cgtggtctgg attactataa
     7321 ccgtaccgtt ttcgagtggg tgactaacag tctcggctcc cagggcaccg tgtgtgcagg
     7381 cggtcgttat gacggtcttg tggaacaact gggcggtcgt gcaacaccgg ctgtcggttt
     7441 tgcgatgggc ctcgaacgtc ttgtattgtt agtacaggcc gttaatccgg aatttaaagc
     7501 cgatcctgtt gtcgatatat acctggtggc ttcaggtgct gatacacaat ctgcggctat
     7561 ggcattagct gagcgtctgc gtgatgaatt actgggcgtg aaattgatga ccaaccacgg
     7621 cggcggcaac tttaagaaac agtttgcccg tgctgataaa tggggtgccc gcgttgctgt
     7681 ggtgctgggt gagtctgaag tggctaacgg cacagcagta gtgaaggatt tgcgctctgg
     7741 tgagcaaacg gcagttgcgc aggatagcgt agccgcgcat ttgcgcacgt tactgggtta
     7801 aggaaggaga aggacagcgt ggaaatttac gagaacgaaa acgaccaggt agaagcggtt
     7861 aaacgctttt ttgctgaaaa tggcaaagca ctggctgttg gggtgatttt gggcgttggc
     7921 gcactgattg gctggcgcta ctggaacagc catcaggttg attctgcacg ctccgcttct
     7981 cttgcctatc aaaatgcggt taccgcagtg agcgaaggca aaccggatag catcccggcg
     8041 gcggaaaaat ttgctgctga aaataaaaat acttatggtg cgctggcttc tttggaactt
     8101 gcgcagcaat ttgttgacaa aaatgaacta gagaaagctg ccgcccagtt acaacagggg
     8161 ctggcagaca cgagcgatga aaatctcaaa gccgtgataa atctgcgtct tgctcgcgtt
     8221 caggtacagc tcaagcaggc tgatgccgcg ctgaaaaccc ttgataccat caaaggtgaa
     8281 gggtgggctg ccattgttgc cgacctgcgt ggtgaagcat tgctgagcaa aggtgataag
     8341 caaggtgcgc gtagtgcatg ggaagcaggc gtgaaaagcg atgttactcc ggcactgagc
     8401 gaaatgatgc agatgaaaat taataatttg tccatctgag agggacccga tgcaattgcg
     8461 taaattactg ctgccaggac tgctttccgt taccctttta agcggctgtt cgctgtttaa
     8521 cagcgaagaa gatgtggtaa agatgtcccc attgccaacc gttgaaaacc agtttacgcc
     8581 gaccacggcg tggagcactt ccgttggtag cggcattggc aacttctatt ccaatcttca
     8641 tccggcactg gcggacaacg ttgtctatgc agcggaccgc gctggtttgg taaaagcgct
     8701 gaatgcggat gatggcaaag aaatctggtc tgtcagcctg gccgagaaag atggctggtt
     8761 ctctaaagag cctgcattac tttctggcgg tgtgaccgtg tctggtgggc atgtctacat
     8821 tggcagcgaa aaggcgcagg tttacgcgct gaataccagc gatggtactg tggcatggca
     8881 aactacagtc gcgggtgaag cactttcgcg cccggtggtc agcgacggtc tggtgttaat
     8941 ccacaccagt aacggtcagt tacaagcgct gaacgaagct gacggcgctg tcaaatggac
     9001 agttaacctc gatatgcctt cgctctcttt gcgtggcgag tctgcgccgg caacggcttt
     9061 tggtgcggct gtcgtgggtg gcgataatgg tcgcgtcagc gcagtgctga tggaacaggg
     9121 ccagatgatt tggcagcagc gtatttccca ggcgaccggt tctaccgaaa ttgaccgtct
     9181 gagcgatgtt gacacgactc ccgtcgttgt taacggcgtt gttttcgcgc tggcctataa
     9241 tggtaacctg acggcgcttg atctgcgcag tggtcagatt atgtggaaac gcgaactggg
     9301 ttcggtgaat gatttcatcg tcgatggcaa tcgcatctat ctggtcgatc aaaatgaccg
     9361 ggtgatggcg ttgaccattg atggcggcgt tacgctgtgg gcacaaagcg atctgctgca
     9421 tcgcctgctg acttctccgg tgctgtataa tggcaacctg gtggtcggtg acagtgaagg
     9481 ttatctgcac tggattaacg tcgaagatgg tcgtttcgtt gcccagcaaa aagttgatag
     9541 ttccggtttc cagactgaac cggttgccgc tgacggcaaa ctgctgatcc aggcaaaaga
     9601 cggaaccgtg tactctatta cacgttaatc gtctctgtcg ttcactttga aaacggctcc
     9661 tggacagggg ccgttttcct gtttttaaca acgacgcgaa tatagcgtgc gttgtctgat
     9721 gatttataaa aatgaggctt taaacatggt acctgtggtc gcgcttgtcg ggcgccctaa
     9781 cgtaggaaaa tccacgttat ttaaccgtct aactcgcacc cgagatgcgc tggttgcgga
     9841 tttcccgggt ctgactcgtg accgtaagta cggtcgtgcg gaaattgaag gccgtgagtt
     9901 tatctgtatt gataccggcg ggattgatgg cacagaagac ggtgtagaaa cccgcatggc
     9961 ggaacagtcg ctgctggcga ttgaagaagc ggacgtcgta ctgtttatgg tggatgcgcg
    10021 cgcgggcctg atgccggcag atgaagcgat tgccaaacat ctgcgctccc gtgaaaaacc
    10081 gaccttcctg gtggcaaaca aaactgacgg tctggatccc gatcaggcag tggttgattt
    10141 ctactcgctt ggtttaggtg aaatctaccc gatcgccgcg tctcacggtc gtggcgtatt
    10201 aagtctgctg gagcatgtgc tgctgccgtg gatggaagat ctcgcaccgc aagaggaagt
    10261 cgacgaagac gctgaatact gggcgcaatt tgaagcggaa gagaacggcg aagaagaaga
    10321 ggaagacgac ttcgacccgc aaagtctgcc gatcaaactg gcgattgtgg gtcgtccgaa
    10381 cgtaggtaag tctacactca ctaaccgtat tcttggagaa gagcgcgttg ttgtttacga
    10441 catgcctggc acgacgcgtg acagcatcta catcccaatg gaacgcgatg gacgtgagta
    10501 tgtgctcatt gacaccgctg gcgtacgtaa acgcggcaaa atcaccgatg ctgtagagaa
    10561 attctccgta atcaaaacgt tgcaggccat tgaagacgcc aacgtggtga tgttagtgat
    10621 tgatgcgcgc gaaggtattt ccgatcagga tctctcgctg ctgggcttta ttctcaatag
    10681 tgggcgctca cttgtcattg tggtgaataa gtgggatggc ctgagccagg aagtgaaaga
    10741 gcaggtgaaa gaaacgctgg acttccgtct gggctttatc gattttgctc gtgtgcactt
    10801 tatctctgcc ttgcacggca gtggtgttgg taacttgttt gaatcagtac gtgaagcgta
    10861 tgacagctcc acccgtcgtg tggggacctc tatgctgacg cgcatcatga cgatggctgt
    10921 tgaagatcac caaccgccgc tggtacgcgg tcgtcgtgtg aagctgaaat atgcccacgc
    10981 tggtggttat aacccgccga ttgtggtgat tcacggtaat caggtgaaag acctgcctga
    11041 ttcctacaag cgctacttga tgaactactt ccgcaaatcg ctggacgtaa tgggatcgcc
    11101 gattcgtatt cagttcaaag aaggggaaaa cccgtatgcg aacaagcgta acaccctgac
    11161 gccaacccag atgcgtaaac gtaagcgtct gatgaagcac atcaagaaaa ataaataatt
    11221 atccgtaagt attttatctg ccattccctc tatgaatgta gagggaatgg ctaccggagg
    11281 aattcatatg gaactgcatt gtccaaaatg tcagcatgtg cttgatcagg ataacggcca
    11341 tgcgcgttgc ccatcctgcg gggaatttat tgaaatgaaa gctctttgtc cagactgcca
    11401 tcaaccgttg caggtgttaa aagcctgcgg tgcagtagat tacttctgtc agcacgggca
    11461 tggcttgata tcgaaaaaac gcgttgaatt cgtgctggct taatgaacct ttttacgcga
    11521 tttttttacc ggctggatgt tttttacttc actttctatc cagccgtctt ccagacgtgt
    11581 ggttagcatt tcacccgctt taacttgctt cacttttttc agtacattgc cgtcagtagc
    11641 agtagtaacg ctgtatccac gcgccagcgt tgacagtggg cttacggctt cgaggtgcgt
    11701 tactgcatta ccgaaacgtt cacgcgtggc gctaagctgt gcgcgcagga tttctgctaa
    11761 acgatattcc agttgctgaa tgcgcgtttg cgtgcgatga atcttcggct gtggattttg
    11821 ctgattcagc cgctgtgtta atcgctgctg ctgttgcccg gcacgcttaa gctgattttc
    11881 cagcgcaaag ctcatccgtt tttgcaggcg ttcaagcatg gtttgctggc gtgccagccg
    11941 gagctgcgga tgctgctgct gcaagcgatg atgaatctgc gtaaagcgac gcgtacggtt
    12001 ggcgagataa taatccatcg ccatctccag ccgttgacgg gccgattgca cctggcgcag
    12061 taactcttgc tgattacggc tcactacttc agcggcggca gacggcgttg gcgcacgcag
    12121 atcggcaaca aaatcggcaa tggtcacatc cgtctcatgc ccgacggcgc tgacgaccgg
    12181 aatgcggctg gcaaaaatcg cccgcgctac gcgttcgtcg ttaaaactcc ataaatcttc
    12241 cagcgaaccg ccgccgcgcc caacgatcaa cacgtcgcac tcattgcgct gattcgccag
    12301 ttcaatggcg cgaacaattt gccccggcgc gtcatcgccc tgaacggagg tggggtagat
    12361 gatcaccggt agagaaggat cgcgacgttt taacacatgc aaaatatcat gtagcgcagc
    12421 accggttttt gaggtgatca caccaacgca atgcgcaggg gagggaagtg gttttttgta
    12481 ttgctgatcg aacaaacctt cagcctgcaa cttcgctttg agctgttcgt acttctgttg
    12541 cagcagccct tcaccggccg gctgcatact ctcaacgatt atctggtagt cgccgcgcgg
    12601 ctcgtagagc gtaatattgg cgcgaactaa aacttgttgc ccatgctgcg gacggaaggt
    12661 gacccggcgg ttgctgttgc ggaacatcgc gcagcgtacc tgggcggtgt cgtctttgag
    12721 tgtaaagtac cagtgaccgg aagccggttg tgtgaaatta gagatttcgc cgctgatcca
    12781 aacctgtccc atctcatgct caagcagcag acgaaccgtt tgattcaggc gactaacggt
    12841 aaaaattgca ggggattgag aaggtaacat gtgagcgaga tcaaattcta aatcagcagg
    12901 ttattcagtc gatagtaacc cgcccttcgg ggatagcaag cattttttgc aaaaaggggt
    12961 agatgcaatc ggttacgctc tgtataatgc cgcggcaata tttattaacc actctggtcg
    13021 agatattgcc catgctacgt atcgctaaag aagctctgac gtttgacgac gttctcctcg
    13081 ttcctgctca ttctaccgtt ctgccgaata ctgctgacct cagcacccag ctgacgaaaa
    13141 ctattcgtct gaatatccct atgctttccg cagcaatgga taccgtaacg gaagcgcgcc
    13201 tggctattgc tctggctcag gaaggcggta tcggctttat ccacaaaaac atgtccattg
    13261 aacgccaggc agaagaagtt cgccgtgtga aaaaacacga atctggtgtg gtgactgatc
    13321 cgcagactgt tctgccaacc acgacgctgc gcgaagtgaa agaactgacc gagcgtaacg
    13381 gttttgcggg ctatccggtc gttaccgaag aaaacgaact ggtgggtatt atcaccggtc
    13441 gtgacgtgcg ttttgttacc gacctgaacc agccggttag cgtttacatg acgccgaaag
    13501 agcgtctggt caccgtgcgt gaaggtgaag cccgtgaagt ggtgctggca aaaatgcacg
    13561 aaaaacgcgt tgaaaaagcg ctggtggttg atgacgaatt ccacctgatc ggcatgatca
    13621 ccgtgaaaga cttccagaaa gcggaacgta aaccgaacgc ctgtaaagac gagcaaggcc
    13681 gtctgcgtgt tggtgctgca gttggcgcag gtgcgggtaa cgaagagcgt gttgacgcgc
    13741 tggttgccgc tggcgttgac gttctgctga tcgactcctc ccacggtcac tcagaaggcg
    13801 ttttgcagcg tatccgtgaa acccgtgcta aatatccgga tctgcaaatc atcggcggca
    13861 acgtggcaac agctgcaggt gcacgcgctt tggcagaagc tggttgcagc gcggttaaag
    13921 tcggtatcgg ccctggttct atctgtacga ctcgtatcgt tactggcgta ggtgttccgc
    13981 agatcactgc cgttgctgac gcagttgaag ccctggaagg caccggtatt ccggttatcg
    14041 cagatggtgg tattcgcttc tccggcgaca tcgccaaagc tatcgccgct ggcgcaagcg
    14101 cggtgatggt aggttccatg ctggcgggta ctgaagaatc tccgggtgaa atcgaactct
    14161 accagggccg ttcttataaa tcttaccgtg gtatgggttc cctgggcgcg atgtccaaag
    14221 gttcctctga ccgttatttc cagagcgata acgctgccga caaactggtg ccggaaggta
    14281 tcgaaggtcg cgtagcttat aaaggtcgcc tgaaagagat cattcaccag cagatgggcg
    14341 gcctgcgctc ctgtatgggt ctgaccggct gtggtactat cgacgaactg cgtactaaag
    14401 cggagtttgt acgtatcagc ggtgcgggca ttcaggaaag ccacgttcac gacgtgacca
    14461 ttactaaaga gtccccgaac taccgtctgg gctcctgatt ctcttcgccc gacttcatgt
    14521 cgggcgattt atattatctg tttcacttgc ctcggaataa gcgtcaatga cggaaaacat
    14581 tcataagcat cgcatcctca ttctggactt cggttctcag tacactcaac tggttgcgcg
    14641 ccgcgtgcgt gagctgggtg tttactgcga actgtgggcg tgggatgtga cagaagcaca
    14701 aattcgtgac ttcaatccaa gcggcattat tctttccggc ggcccggaaa gtactactga
    14761 agaaaacagt ccgcgtgcgc cgcagtatgt ctttgaagca ggcgtaccga tattcggcgt
    14821 ttgctatggc atgcagacca tggcaatgca gttgggcggt cacgttgaag cctctaacga
    14881 acgtgaattt ggctacgcgc aggtagaagt cgtaaacgac agcgcactgg ttcgcggtat
    14941 cgaagatgcg ctgaccgcag acggtaaacc gctgctcgat gtctggatga gccacggcga
    15001 taaagttacc gctatcccgt ccgacttcgt caccgtagcc agcaccgaaa gctgcccgtt
    15061 tgccattatg gctaacgaag aaaaacgctt ctatggcgta cagttccacc cggaagtgac
    15121 tcacacccgc cagggtatgc gcatgctgga gcgttttgtg cgtgatatct gccagtgtga
    15181 agccctgtgg acgccagcga aaattatcga cgatgctgta gctcgcatcc gcgagcaggt
    15241 aggcgacgat aaagtcatcc tcggcctctc tggtggtgtg gattcctccg taaccgcaat
    15301 gctgctgcac cgtgctatcg gtaaaaacct gacttgcgta ttcgtcgaca acggcctgct
    15361 gcgtctcaac gaagcagagc aggttctgga tatgtttggc gatcactttg gtcttaacat
    15421 tgttcacgta ccggcagaag atcgcttcct gtcagcgctg gctggcgaaa acgatccgga
    15481 agcaaaacgt aaaatcatcg ggcgcgtttt cgttgaagta ttcgatgaag aagcgcagaa
    15541 actggaagac gtgaagtggc tggcgcaggg caccatctac cctgacgtta tcgaatctgc
    15601 ggcttctgca accggtaaag cacacgtcat caaatctcac cacaacgtgg gcggcctgcc
    15661 gaaagagatg aagatgggcc tggttgaacc gctgaaagag ctgttcaaag acgaagtgcg
    15721 taagattggt ctggagctgg gcctgccgta cgacatgctg taccgtcatc cgttcccggg
    15781 accaggcctt ggcgttcgtg ttctgggtga agtgaagaaa gagtactgtg acctgctgcg
    15841 ccgtgctgac gccatcttca ttgaagaact gcgtaaagcg gacctgtacg acaaagtcag
    15901 ccaggcgttc accgtcttcc tgccagtacg ttccgttggc gtaatgggcg atggtcgtaa
    15961 gtatgactgg gttgtctctc tgcgtgctgt cgaaaccatc gactttatga ccgcacactg
    16021 ggcgcatctg ccgtacgatt tcctcggtcg cgtttccaac cgcattatca atgaagtgaa
    16081 cggtatttcc cgcgtggtgt atgacatcag cggcaagccg ccagctacca ttgagtggga
    16141 atgattttga tccctgcact atgaatgaac aaaaccctcc gttactacag agggtttttt
    16201 atcttcaaga atcataggct tgaagttact aacatcgatt aattaaacca gctgtccgat
    16261 ttgttctctt ctgctttgcc cacgcttttc atcagatcgc gaccgccttc agtcatattt
    16321 ctgtttgcgt cagcttcaga ttgcaccaca tcggtttgcg cagctttgtg cttcagttcc
    16381 tgatcgataa attcgttttc gcgcttaacg cgggcttctt ctttcgccag cgccagtttt
    16441 tgtttctgaa tctctaagct gcgtagctca tcttcataac tttgatcgcg ttttttgtcc
    16501 gcagtggctt cggcgtccag tttatcctga cgagctttct tatttgccgc tgccgttgcc
    16561 gctcttttgt tcgcggcggc ctgggcattt gcgcgacgtt gcttctcttg ctggatttcc
    16621 ctgttgcgct ccgcgaccca ttcgtcatgc tgcctttgct cttcattttt accttgctgt
//
LOCUS       DAJVQH010000084        16565 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJVQH010000084 DAJVQH010000000
VERSION     DAJVQH010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.084"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..439
                     /locus_tag="OQM87_002773"
     CDS             <1..439
                     /locus_tag="OQM87_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCS1508844.1"
                     /translation="LRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIR
                     QLLAQRHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINA
                     VADMFKGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            430..903
                     /locus_tag="OQM87_002774"
     CDS             430..903
                     /locus_tag="OQM87_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCS1508845.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1010..1549
                     /gene="dnaT"
                     /locus_tag="OQM87_002775"
     CDS             1010..1549
                     /gene="dnaT"
                     /locus_tag="OQM87_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCS1508846.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPVSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            1552..2289
                     /gene="dnaC"
                     /locus_tag="OQM87_002776"
     CDS             1552..2289
                     /gene="dnaC"
                     /locus_tag="OQM87_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCS1508847.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2338..2832
                     /locus_tag="OQM87_002777"
     CDS             2338..2832
                     /locus_tag="OQM87_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCS1508848.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3086..5377
                     /gene="opgB"
                     /locus_tag="OQM87_002778"
     CDS             3086..5377
                     /gene="opgB"
                     /locus_tag="OQM87_002778"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCS1508849.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(5515..6537)
                     /gene="lgoD"
                     /locus_tag="OQM87_002779"
     CDS             complement(5515..6537)
                     /gene="lgoD"
                     /locus_tag="OQM87_002779"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCS1508850.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            6676..7590
                     /locus_tag="OQM87_002780"
     CDS             6676..7590
                     /locus_tag="OQM87_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1508851.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGILTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            7804..9165
                     /locus_tag="OQM87_002781"
     CDS             7804..9165
                     /locus_tag="OQM87_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1508852.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9214..10878)
                     /gene="tsr"
                     /locus_tag="OQM87_002782"
     CDS             complement(9214..10878)
                     /gene="tsr"
                     /locus_tag="OQM87_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCS1508853.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(10997..11443)
                     /gene="hpaR"
                     /locus_tag="OQM87_002783"
     CDS             complement(10997..11443)
                     /gene="hpaR"
                     /locus_tag="OQM87_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCS1508854.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            11715..13004
                     /locus_tag="OQM87_002784"
     CDS             11715..13004
                     /locus_tag="OQM87_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCS1508855.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPQVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13001..14467
                     /gene="hpaE"
                     /locus_tag="OQM87_002785"
     CDS             13001..14467
                     /gene="hpaE"
                     /locus_tag="OQM87_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1508856.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVDDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLHQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            14469..15320
                     /gene="hpaD"
                     /locus_tag="OQM87_002786"
     CDS             14469..15320
                     /gene="hpaD"
                     /locus_tag="OQM87_002786"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCS1508857.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15330..15710
                     /locus_tag="OQM87_002787"
     CDS             15330..15710
                     /locus_tag="OQM87_002787"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCS1508858.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            15778..>16565
                     /gene="hpaH"
                     /locus_tag="OQM87_002788"
     CDS             15778..>16565
                     /gene="hpaH"
                     /locus_tag="OQM87_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCS1508859.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGHPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSI"
BASE COUNT         4007 a         4444 c         4205 g         3909 t
ORIGIN      
        1 attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg cctgtttctg
       61 taaactcaat aacggtggct gggatggtgc cgtcatcacc ttctttgcca gtacgaccgc
      121 gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccacagatca acttttgcct
      181 taccgctttc gccgccacca ccatttccgg attgcttttg caactcccca ctttcagcaa
      241 tacccccacc attgcgatgg ccgccagcgt tctgctgcta gtgccgggct ttccgttgat
      301 taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat
      361 cgccagtctg ctgacactgg ctacctgcgt cggcgtagtg atggcactga cgatttgggg
      421 tctacgcgga tgggtgtgat cgaatttctg ttagcgttgg cgcaggatat gatcctcgcc
      481 gccattcctg cggtcggctt tgcgatggtg ttcaacgttc ccgtgcgggc gttacgctgg
      541 tgtgcgctgc ttggctcgat aggtcatggt tcccgaatga tcttgatgac cagcgggttg
      601 aatattgagt ggtcaacctt tatggcttct atgctggtcg gtaccattgg tattcaatgg
      661 tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc
      721 ccgggcatat cggcttatac cgcaatgatt tcgtcggtaa aaatcagcca gttaggttac
      781 agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt
      841 gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta
      901 taaaattgcc tcgcttaagc acacggatga gagacagcct cctctcctcc gtgtgttact
      961 ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaacagta tgtcttccag
     1021 agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc aaaccgttct
     1081 ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg cgttttatgc
     1141 cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaagctag cgcgtccggt
     1201 aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagccgctc ccgttgctgt
     1261 acccatgggg aaattcgcca tgtatccgga ctggcaaccc gatgccgatt ttatccgcct
     1321 ggcggcgcta tggggcgtgg cgctaagaga gccggtgacc accgaagaac tggcctcatt
     1381 cattgcctac tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc aacaaaaact
     1441 ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac gagatgtgaa
     1501 tacggtcagt gaacctgaca gccaaattcc accaggattc agagggtaac gatgaaaaac
     1561 gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa acctgcattc
     1621 aaaacgggtg aagagctact ggcctggcag aaagaacaag gggtgatccg ctccgccgct
     1681 ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc cggtattcgt
     1741 ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg gcagatgaat
     1801 gcgttaagca aagcgcgcca gtatgtcgaa gagtttgacg gcaacatcgc cagcttcatc
     1861 ttttctggta agccaggaac cggcaaaaac catctggcgg cggcaatctg caacgagctg
     1921 ctgctacgcg gtaaatccgt attgatcatc accgtggccg atattatgtc ggcgatgaaa
     1981 gataccttca ggaatagcgg taccagcgaa gaacaactgc ttaacgatct gagcaacgtt
     2041 gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga aaaagtgatc
     2101 atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat gctgaccaac
     2161 agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg tatgcgcctg
     2221 ggtaacagtt tatgggtgat cttcaactgg gatagctacc gtagccgggt aacgggtaaa
     2281 gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttccaccttg agtaatgatg
     2341 atgaaaacta ttaaacatct tctgtgctgt gccattgccg ccagcgcatt aatttccacc
     2401 ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga acttggcaac
     2461 caaaacagca cgacacagga aggcggttgg tcgctcgcgt cattaactaa cttgcttagc
     2521 agcggaaacc aggccttaag cgcagataac atgaacaatg ccgcaggcat tctgcaatac
     2581 tgcgcgaagc aaaagctggc ttcggtaacc gatgccgaaa acatcaagaa ccaggtgctg
     2641 gaaaagctgg gcctgaacag tgaagagcaa aaagaagaca ccaactatct ggacggtatt
     2701 cagggtttgc tgaaaacaaa agatggtcag caactaaatc tggataacat cggaacgact
     2761 ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca ggggctgaac
     2821 ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac gcggctctct
     2881 cctgcccttt tccctgctcc tggacggttt tccccctgtt acacgggaag cgaccgcgat
     2941 caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga cactagaatt
     3001 gcagcaatat ggcgacgcca ttacattgtg cttctcaaaa agcttaaacg ttggctaaac
     3061 ggcccggttt agtgaggatc gcccgttgtc agaactactc tctttcgccc tttttctcgc
     3121 ctctgtgctg atttacgcat ggaaagcggg acgtaacacc tggtggtttg cagccacgtt
     3181 aacggtgctg gggctatttg tcgttttaaa tatcaccctg tttgccagcg actattttac
     3241 tggcgatggt attaacgacg cggttctcta taccttaacc aacagcctga ccggtgctgg
     3301 cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg gggctgacgg cggtgttcgg
     3361 tgcgctgggc tggatcctgc gccgtcgtcg tcatcatccg caccattttg gttacagcct
     3421 gctggcgctc ttactggcgc tgggttcggt ggacgccagc ccggcatttc gtcagataac
     3481 ggaactggtg aaatcccagt cacgcgatgg cgacccggac tttgcggctt attataaaga
     3541 gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctacg gcgaaagtct
     3601 cgagcggacc tattttgata acgaagcttt cccggatctc acgcctgaac tgggcgcgtt
     3661 gaaaaatgaa ggcctggatt tcagccacac gcagcagctg ccaggaacgg attacacgat
     3721 tgcgggcatg gtggcttctc agtgcggcat accgctgttc gccccctttg aaggcaacgc
     3781 ctccgcctct gtctccagct tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa
     3841 ctcgggttat cagaactatt tcgtgcaggg cgcaaatctg cgttttgccg gtaaagatgt
     3901 gttcctgaaa tcgcacggct tcgaccactt atacggctca gaagagctga aaagcgtggt
     3961 agccgacccg cactatcgca acgactgggg attctacgac gataccgttc tcgatgaagc
     4021 gtggaaaaag tttgaagagc tttcccgctc aggtcagcga ttctcactgt ttaccctgac
     4081 agtcgatacc catcacccgg atggttttat ctcgcgcacc tgtaaccgca aaaaatatga
     4141 ttttgacggt aagccgaatc agtcattcag cgcggtaagt tgcagccagg agaatatcgc
     4201 gacgtttatc aacaaaatca aagcgtcacc gtggtttaaa gatactgtca tcgtcgtctc
     4261 ttctgaccat ttagcgatga acaacacggc gtggaaatac ctcaataagc aggatcgcaa
     4321 taacctgttt tttgtcattc gtggcgacaa gccgcagcaa aagacgctgg cagtgaagcg
     4381 taacacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata actatctcgg
     4441 acttggtcgt agcagtttat ccgggcagtc gatgtcggaa atcttcctca atatcaaaga
     4501 gaaaacattg gcgtggaagc cagatatcat ccgcctgtgg aaattcccta aagagatgaa
     4561 agagttcacc atcgaccagc agaaaaacat gattgccttc tcgggtagcc atttccgttt
     4621 gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa gcgaatactc
     4681 agcaccgctg cgtttccagc tggccgattt cgctccacgc gacaatttcg tctgggttga
     4741 cagttgctac aagatggcac aactctgggc tccggaactg gcactttcca ccgactggtg
     4801 tgtctcgcag gggcagcttg gcggtcagca aattgttcag catgttgaca aaacaacatg
     4861 gaagagcaag acggcattta aagatacggt catcgacatg gcgcgttaca aaggcaatgt
     4921 cgatacgctg aagattgttg ataacgatat tcgctacaaa gccgacagtt tcatctttaa
     4981 cgtcgccggt gcgccagaag aggtgaaaca gtttagcggg atttcccgtc cggagtcgtg
     5041 gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa atcgagtaca agcatccgct
     5101 gccgaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg ccagccgtcc
     5161 tattccggta cgcgtaggca atgaagaaca aactcttgtg ctgggcaatg aagtgaccac
     5221 caccacactg catttcgata acccaaccga tgccgacaca ctggtaattg tgccgccgga
     5281 acctgtctca accaacgaag ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg
     5341 catggtggaa attaaagtgg tagaacgtga agggtaattc ctgactgatg tgtaggtcgc
     5401 atagggtgcg acctacacgc cttctccggc ttacaacaca ttattaagaa acaaaaaaaa
     5461 cagcgcccaa cacttccccg gacgctgcat ggcaacttta ataatctcgc cttatcagaa
     5521 agtaattaca cccttaatca actcacgatt gttaatcaca tcgcgctcgt aggtttctgc
     5581 cagcgtggcg aacggatagc gatgggttaa catcatgtcg gcagtgattt tcccttccgc
     5641 catcagtcga ccgactttag cgaaatcttc cggcgtggcg ttgcggctgc ccatcatcgt
     5701 cgtttctttt ttatggaatt ccggatcgga gaactgcaac tcacctttaa acagaccgac
     5761 aaataccacc gtgccgccgt gacgaatcag attcacggta ttattcatcg catgttgatt
     5821 acctgtcgcg tcgatcactt tctgcgccag cgaaccacca aactgcgccc gcagctgcgc
     5881 gtcaaaatct tcggctgacg ggtccagcac gggcaactcc agacgcgttg ccacatgttc
     5941 acggcgcgcc ggactggtat ccgccaccac cacctgtgcg ccatcggctt tagcgattgc
     6001 cgccgcgccc agaccgattg gccccgcccc gaccaccagc acctgctcgc cgggagcaat
     6061 ggctgcgcga cgcaccgcat gagcgctaat agcgaaaggt tcaatcaatg cggccgcctg
     6121 cgggtcaata ccgtctgcgg gcaaaatgtt cgccaccggc acgctcaaat actcactaaa
     6181 accgccatcc tgatgcacgc caatgactga aattttttca cagcagttgg tacgcccgct
     6241 tttacacgcc gggcattgct gacaggcaac ataagggatc acagcaactt gctgaccatt
     6301 tttaagatca gcaatatttt tacccagccc aacaatctcc ccacatattt catggcctaa
     6361 aacccgtgga taactaaaaa atggttgatt tccaccccag gcatgaatat cggtaccgca
     6421 aatcccgaca gacttaattt ttattaatgc ttcattgtca cccggaatag gtatctcgcg
     6481 ttgtttccag actaattctt tcggctgctg gcaaattaaa acattcatcg tagacatgat
     6541 tcggctctcc attttttgtt gccttagata tcaatgaaaa tgaaaaatgg cagcggaacg
     6601 gaaaatctgt tttgtgaaaa cacacgcata aaaatgtttt aaatcgggtt ttaatggact
     6661 cacaaggagg aaaatatgag tcgttcacaa aatttacgcc acaatgtgat taaccaggtc
     6721 attgatgata tggcgcgggg tcatatccct tccccacttc catcgcaaag tgcgctggcg
     6781 gaaatgtaca acatcagccg cactacagtg cgtcatattc tcagccaatt acgcgaatgc
     6841 ggcatcttaa cgcaggttgg aaacgactat gttattgcgc gtaaacctga ccatgatgac
     6901 ggtttcgcct gtactaccgc ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt
     6961 ttcacgatga taaaccagcg tcagttacgc cctagggaaa ctttttctga actgcaactg
     7021 gctcgggccg cgggtgtcag cccggtggtt gtaagagaat atcttttaaa attcggccgt
     7081 tataatctta ttcaaagcga aaagcgcggc cagtggagca tgaagcaatt cgatcaatcc
     7141 tacgccgagc agctgtttga gctacgcgaa atgctcgaaa cacattcact tcagcacttt
     7201 ctcaacctgc ctgatcatga tccccgctgg ctgcaggcga aaaccatgct agaacgtcat
     7261 cggctactgc gtgacaacat cggcaatagc tttcgcatgt tctcgcaact tgacagagat
     7321 tttcactcct tactgctttc agctgccgac aatatctttt ttgatcaatc gcttgagatc
     7381 atctcagtca tctttcattt tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat
     7441 atcattgccg tcgatgaaca tatgaccatt ctcagcgctc tgatttgccg cagtgacctt
     7501 gatgccaccc ttgcgctccg taaccatttg aattcagcta aacaatcaat gattcgatcc
     7561 attaatgaga acacgcgtta tgctcattaa gtacggatta aaaacagcaa actattagcg
     7621 ggtgcacggt tttatcaaaa gataaatacc ggaaattccc gcttacctat gctcacaatc
     7681 agtcgactaa aaatgttttt gtgagcggta ataaagtcct aaaactttaa cctgccgtta
     7741 atgtcaacct tgcgtaatgc acaattacgt aaacaattaa aattataaat acaggagttc
     7801 ggcgtggaaa aagaaaatat caccctcgat ccgcgttctt catttactcc atcttcgtcg
     7861 gcagatattc ccgtgccacc agatggatta gttcaacgca gtacccgaat taaacgcatt
     7921 caaaccaccg ccatgttgtt attatttttt gcggcggtaa tcaattatct cgaccgcagt
     7981 tcgctgtcgg tagcaaattt aacgattcgt gaagaattgg gattaagtgc caccgaaatc
     8041 ggcgctttgc tctccgtgtt ttcactcgct tacggcattg cgcaacttcc ttgcggccca
     8101 ctattggatc gtaaaggccc acgcctgatg ctgggactgg ggatgttctt ctggtcactg
     8161 ttccaggcca tgtctggcat ggtgcacagc tttacgcagt tcgtgttggt gcgtatcggt
     8221 atggggattg gtgaagcgcc gatgaaccca tgcggtgtaa aagtcattaa cgactggttc
     8281 aacatcaaag agcgcggacg cccgatgggc ttcttcaacg cagcttctac cattggcgtt
     8341 gcggtaagcc caccgattct ggcggcaatg atgctggtga tgggctggcg cgggatgttt
     8401 attaccattg gtgtactggg gatttttctc gccatcggct ggtatatgct ctatcgcaac
     8461 cgcgagcacg tagaactgac tgccgttgaa caagcttatc tcaatgcagg tagcgtcaat
     8521 gcccgccgag atccgctcaa ttttgccgaa tggcgcagcc tgttccgtaa ccgcacaatg
     8581 tggggaatga tgctcggatt cagtggcatc aactacactg cgtggctgta tctggcctgg
     8641 cttcctggtt acctgcaaac agcctataac ctggatttaa aaagcacagg gttgatggcg
     8701 gctatccctt tcctgtttgg ggctgccggg atgctggtca acggttacgt tactgactgg
     8761 ctggtcaaag ggggaatggc tccgattaaa agccgtaaga tctgcattat taccgggatg
     8821 ttctgttctg ccgcctttac gctgatagta ccacaagcga caacatccat gatggcggtt
     8881 ctgctgattg gcatggcact gttctgtatt cactttgccg gaacatcctg ctggggcttg
     8941 atccacgtcg cagttgcttc tcgcatgact gcgtcggtgg gcagtatcca gaactttgcc
     9001 agcttcatct gcgcctcttt cgcgccgatc attactggtt ttattgttga tacaacccac
     9061 tcattccgtc tggcactaat catttgcggt tgcgtcaccg cagcgggagc actggcgtac
     9121 atcttcctgg ttcgccagcc gatcaacgac ccgcgtaaag attaaactcc ctatgcccga
     9181 taacattttg cttatcgggc atttttatgg cgattaaaac gtttcccagt tctccccgct
     9241 atctgccact gccattttac gcggcgtagc tggcgtcacg gtttttacca cagccgatgt
     9301 ttcacgctgc tgttgctgct gttgctgaat ccggaacact gccacagctt cggtcaggcg
     9361 actggcctgc tcttccagcg cggcggcggc agcggcagat tcttccacca gcgcggcgtt
     9421 ctgttgagtt acccggtcca tctcagcaac cgctaagcca acctgatcga taccacggct
     9481 ctgctcatca gaagcagaag ctatttcgcc cataatgtcc gtcacgcggg tcacagcact
     9541 gacaatctcc gccattgttt ccccggcgct ttcgaccagc gtagagccaa catccacttt
     9601 ccccaccgag tcttcaatca ggcttttaat ttcacgagcc gcctgggcgc tacgctgggc
     9661 cagattacgc acttctcccg caaccaccgc aaaaccacgt ccttgctcac ccgcacgcgc
     9721 tgcttcaacc gccgcgttca aagccagaat attggtctgg aaggtaatgc cgtcaattac
     9781 gctgataata tcggcgattt tctgcgaact ggtggagata tcgcgcatag tctgcaccac
     9841 gttatctacc actttgccgc cgcgttgcgc cgtttcagaa gcacttaatg ccagatggct
     9901 ggcctggcgc gcattctcgg cgttctgttt caccgttgcg gtcagttgct ccatgctggc
     9961 tgccgtctct tccagcgaag cggcctgttg ctcggtgcgc gaagagagat cattattgcc
    10021 ggtagcgatt tcgctggcac cgctatagat ggcattggcc ccgttgcgca catcaccgac
    10081 ggtacgcatc agctctccct gcatatggcg caaactctct gccagttgcc ccatctcatt
    10141 agagccatcc acctcaatcg gtttcaccag atcgccgcct gcaatatgac gaatgctgtc
    10201 aatcaggcga ttcattggcg ctaccagcga ggctttaata ccgaaccaga cggcgaagat
    10261 gaccgccagt acgacgatca tcacgcccac cagaatccac atcgcctggc tgtaggaggc
    10321 attgttatcg ctgacggcga tatcatagag ccgatcgttt tgctccatgt aagccacata
    10381 ctgcttctcg aaaccgtcct gatatccctg ggtcggctga tcaaagaact cgttgatttt
    10441 gcctgcacct aacagttgga tcagctccgc cagcgcattg tgataaatat cgtaattacg
    10501 tttgatctct gccgctgcgg cggtgctctg acgcgggtca cgcggcaacg cttcgtaatc
    10561 cgcccagttt ttttccgcct gtttcagcga aatactggca ctctgcatca gctcagcaac
    10621 ggttgaaccg ctaccaatat tattctgatc catcatgtag cggatacccg cgcggttgag
    10681 ggtgttacgc gtctgcaaca acgcgaccca gctgccattc agcgtggatt gctgctggcg
    10741 aatggtttgt aaaacagtga aattttcttt gtcattcttt aaggcattaa aaaacagacc
    10801 gcctgatgtc agttgtaaaa ggccaaaaac ggccaaaacc agcagtaagc tggtcacgat
    10861 cttgatacgt tttaacatgg tttctctttc ctgtggaccg atgcaagatt ttcggcctgg
    10921 aaagcaaaaa ctttatgaaa aggtgtaacc cgccatccat ggaaaaacca ggcgctggtt
    10981 atcagatact aaaaagttat tcattatcgc ccgggatgtc ttcttgtcgg gaattgccca
    11041 gagcaataaa ttcctctaac agatgggtta actgttgcat tttctcggca gtaaactgag
    11101 cttcaatctg ccgataggct tcttcaatct gtgtctgagc gcggttatac agcgcctgcc
    11161 cctctttggt cagcgagata tagagcttcc gctgatcatt gatcggcttt aatcgcaaca
    11221 ctaaaccgtc gcgttccatc cgcgtgagga tcccggtcag gcttgggcgc aaaatgcagg
    11281 cgcgatacgc cagatcgtga aaatccattg atgggctttc cgccagaata cgcacgatgc
    11341 gccactgttg ctcagtcaaa ttatgccgtt taacgattgg gcgaaaataa ctcatcgccg
    11401 cttctcgcgc ctggagcaac gcaatggtta gtgagtcgtg cattatcttt cccctgaaaa
    11461 attcaaaatc attaatatag aaacaattaa tcatcacgtt accgggactg agctgccccc
    11521 gcaattcttc attttatcaa aattgatgat attttattaa cttactgata tttaatattt
    11581 tattatcttt aaatgtaaat agtttgttaa ttagatcaca tttatatcac ttacttttgc
    11641 caaactacgc aatcgggaat aaaagtaatc attaacatat taatgattaa gatgatcgaa
    11701 tccgaggagt ggtaatgaaa ggcactatct tcgccgtagc gttgaaccat cgcagccaac
    11761 ttgatgcctg gcaggaagcg ttccagcaat ctccctacaa agccccgcct aaaactgcgg
    11821 tctggtttat taaaccgcgc aatacggtga ttggttgcgg tgaaccgatt ccctttccac
    11881 agggtgaaaa agtactgagc ggtgcgactg ttgcgctgat tgtggggaaa acggcgacga
    11941 aagtacgtga agaagatgcg gcagagtaca tcgccggata tgcgctggct aacgacgtca
    12001 gcctgccgga agagagcttt taccgtccgg caatcaaagc aaaatgtcgt gatggattct
    12061 gccccattgg cgaaaccgtg gctctcagca atgtcgataa tctgactatc tataccgaga
    12121 tcaacgggcg tcctgccgat cactggaata ccgccgattt acaacgtaac gccgcacagc
    12181 tgctgagcgc cctgagcgaa tttgccacgc tgaatccagg cgatgctatt ctgctcggca
    12241 cgccacaggc gcgcgtggaa atacagccaa gcgatcgcgt gcgtgttctc gcagaaggtt
    12301 tcccgccgct ggaaaatccg gtagtggacg aacgtgaagt gaccacgcgc aagagcttcc
    12361 caacgctgcc acacccgcac ggtacgctgt ttgccctcgg cctgaactac gccgaccacg
    12421 ccagcgaact ggaatttaag ccaccggaag agccgcaggt gttcctgaaa gcgccgaata
    12481 ccctcactgg cgacaaccag acctccgtgc gcccgaacaa tattgaatac atgcactacg
    12541 aagcggagct ggtggtagtt attggcaagc aggcgcgtaa cgtcagcgaa gccgatgcca
    12601 tggattatgt cgcgggctac accgtgtgta acgactacgc cattcgcgac tatctggaaa
    12661 actactaccg ccctaacctg cgggtcaaaa gccgcgacgg actgacgccg atgctttcaa
    12721 ccatcgtgcc gaaagaggcg atcccggacc cgcataatct gacccttcgc accttcgtca
    12781 acggcgagtt acgccagcaa ggcaccaccg ccgatctgat cttcagcgtg cccttcctga
    12841 tcgcctactt aagcgaattt atgaccctga atccgggcga catgatcgcc accggcacac
    12901 caaaaggctt atctgacgtg gtgcctggcg atgaagtagt ggtggaagta gaaggcgtgg
    12961 gccgcctggt gaaccgaatt gtgagtgagg aaacagcgaa atgaaaaaag taaatcattg
    13021 gatcaacggc aaaaatgttg caggtaacga ctacttccag actaccaatc cggcaacggg
    13081 tgaagtgctg gcggatgtgg cctctggcgg tgaagcggag atcaatcagg cggtagcggc
    13141 agcgaaagag gcgttcccga aatgggctga tctgccgatg aaagagcgtg cgcgcctgat
    13201 gcgccgtctg ggcgatctga tcgaccagaa cgtgcctgag atcgccgcga tggaaaccgc
    13261 ggacacgggc ctgccgatcc atcagaccaa aaatgtgttg atcccacgcg cttctcacaa
    13321 ctttgaattt ttcgcggaag tctgccagca gatgaacggc aagacctatc cggttgacga
    13381 caagatgctc aactacacgc tggtgcaacc ggtaggcgtt tgtgcgctgg tgtcgccgtg
    13441 gaacgtgccg tttatgaccg ccacctggaa ggtcgcgccg tgtctggcgc tgggcaatac
    13501 cgcggtgctg aagatgtcgg aactctcccc gctgaccgcc aaccgcttgg gtgagctggc
    13561 gctggaagct ggtattccgg cgggcgtgct aaacgtggta cagggctacg gcgcaaccgc
    13621 aggcgatgcg ctggtccgtc atcatgacgt gcgtgccgtg tcgttcaccg gcggtaccgc
    13681 caccgagcgc aacatcatga aaaacgccgg gctgaaaaaa tactccatgg agctgggcgg
    13741 taaatcaccg gtgctgattt ttgaagatgc cgatattgag cgtgcgctgg acgccgccct
    13801 gttcaccatc ttctcaatca acggcgaacg ctgcaccgcc ggttcgcgca tctttattca
    13861 acagagcatc tatccagaat tcgttaaacg ctttgccgaa cgcgccaacc gtctgcgcgt
    13921 ggacgatccg aacgatccga atacccaggt tagggcgctt atcagtcagc aacactggga
    13981 aaaagtctcc ggctatatcc gtctcggcat tgaagaaggc gcaaccctgt tggcgggcgg
    14041 cccggataaa ctgtccgacc tgcctgcgca cctgaaaggc ggcaacttcc tgcgcccaac
    14101 ggtgctggcg gacgtagata accgtatgcg cgttgcccag gaagagattt tcgggccggt
    14161 cgcctgcctg ctgccgttta aagacgaagc cgaaggctta cgcctggcaa acgacgtgga
    14221 gtacggcctc gcgtcgtaca tctggacaca ggatgtcagc aaagtgttac gcctggcgcg
    14281 tggcattgaa gcaggcatgg tgttcgtcaa cacccagaac gtgcgcgacc tgcaccagcc
    14341 atttggcggc gtaaaagcct ccggcaccgg gcgtgaaggc ggtgagtaca gcttcgaagt
    14401 gttcgcggaa atgaagaacg tctgcatttc catgggcgac catccaattc cgaaatgggg
    14461 agtctgatat gggtaagtta gcgttagccg ccaagatcac gcacgtaccg tcgatgtatc
    14521 tctctgaact accaggaaaa aaccacggtt gtcgccaggg tgcgatcgac gggcataaag
    14581 agatcagcaa gcgttgccgg gaaatgggcg tcgataccat tatcgttttc gatactcact
    14641 ggctggtcaa cagtgcttat cacatcaact gtgcagacca ttttgaaggc gtctacacca
    14701 gcaacgagct gccgcatttt attcgcgaca tgacctacaa ctacgagggc aacccggagt
    14761 tggggcagct aattgccgag gaagccttaa agctcggcgt gcgggcaaaa gcgcacaata
    14821 ttcccagcct gaaactggaa tacggcacgc tggtaccgat gcgctacatg aatgaagaca
    14881 agcacttcaa agtggtctcc atttcggctt tctgcacggt tcacgatttt gccgacagcc
    14941 gcaagctggg cgaagcgatt ctgaaagcga tcgaacagta cgacggcacc gtggcggtcc
    15001 ttgccagcgg ttcgttatcg caccgcttta ttgacgatca gcgtgcggaa gaagggatga
    15061 acagctacac ccgcgagttc gaccgccaga tggacgagcg tgtggtgaag ctgtggcgcg
    15121 aaggccagtt caaagagttt tgcaatatgc tgccggagta cgccgactac tgctacggcg
    15181 aaggcaatat gcacgacacg gtgatgctgc tggggatgct cggctgggat aaatacgacg
    15241 gcaaggtgga gtttattacc gagctgttcc caagctctgg caccggtcag gttaacgctg
    15301 ttttcccgct gcccgcgtaa ggacgtttta tgccgcactt tatcgtggaa tgtactgaaa
    15361 atattcgtga agaagcccgc ttacctgagc tgtttgccag tgttaacact gcactggcgg
    15421 caacggggat ttttccgctt gggggaatac gtagccgggc gcactggatc gatacctggc
    15481 aaatggctga tggcaagcac gattacgcct ttgtgcatat gacgctaaaa atcggcagcg
    15541 gacgcagtct ggaaagccgt caggaagtgg gcgaaatgct gtttgatctg attaaaacgc
    15601 acttcgcctc actgatggag agccgttacc tagcgctttc atttgagatt gcagagctgc
    15661 acccgacgtt gaatttcaaa cagaacaacg tacacgcgtt gtttaaataa atttgcacca
    15721 gcttccaggc cggatgataa ggcgctgccg catccggcaa tgccacagga tatcgctatg
    15781 ttcgataaac acacccacac cctgatcgcc cagcgtctgg atcaggcaga aaaacagcgc
    15841 gaacagatcc gcgcgatctc gctggattac ccggagatca ccatcgaaga cgcctacgcc
    15901 gtgcagcgcg aatgggttcg cctgaaaatc gccgaaggtc gcacgctgaa aggccacaaa
    15961 atcggtctga cctcgaaagc gatgcaggca agctcgcaga tcagcgaacc ggattacggt
    16021 gcactgctgg acgacatgtt cttccacgat ggcagcgata tcccgaccga tcgctttatc
    16081 gtgccgcgca ttgaagtgga gctggctttt gtgctggcaa aaccgctgcg tgggccaaac
    16141 tgcacgctgt tcgacgttta caacgccacg gactatgtga tcccggcgct ggagctgatc
    16201 gacgctcgct gccacaacat cgatccggaa acccagcgcc cgcgcaaagt attcgacacc
    16261 atttccgata acgccgccaa cgccggagtg atcctcggag gtcatccgat caaaccagac
    16321 gaactggatc tgcgttggat ctccgccctg atgtatcgca atggcgtgat tgaagaaacc
    16381 ggcgtcgccg ctggtgtgct gaatcatccg gcaaacggcg tggcctggct ggcgaacaaa
    16441 ctcgcaccgt atgacgtaca actggaagcc gggcaaatca ttctcggtgg ttcgttcacc
    16501 cgcccggttc cggcgcgtaa gggcgacacc ttccacgtcg attacggcaa catgggctcc
    16561 attag
//
LOCUS       DAJVQH010000085        16389 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJVQH010000085 DAJVQH010000000
VERSION     DAJVQH010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.085"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..1682
                     /gene="ldtD"
                     /locus_tag="OQM87_002789"
     CDS             <1..1682
                     /gene="ldtD"
                     /locus_tag="OQM87_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1508860.1"
                     /translation="TAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAA
                     RDMQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMM
                     GYLHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQ
                     YAAMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLP
                     GDDTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQ
                     GLGADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQ
                     NGNQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLE
                     SHGYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAI
                     YLHDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYV
                     NIRQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            1863..2411
                     /locus_tag="OQM87_002790"
     CDS             1863..2411
                     /locus_tag="OQM87_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCS1508861.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2438..3085
                     /gene="gloC"
                     /locus_tag="OQM87_002791"
     CDS             2438..3085
                     /gene="gloC"
                     /locus_tag="OQM87_002791"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCS1508862.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3308..4498)
                     /gene="aspC"
                     /locus_tag="OQM87_002792"
     CDS             complement(3308..4498)
                     /gene="aspC"
                     /locus_tag="OQM87_002792"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCS1508863.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(4683..5771)
                     /gene="ompF"
                     /locus_tag="OQM87_002793"
     CDS             complement(4683..5771)
                     /gene="ompF"
                     /locus_tag="OQM87_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCS1508864.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6373..7773)
                     /gene="asnS"
                     /locus_tag="OQM87_002794"
     CDS             complement(6373..7773)
                     /gene="asnS"
                     /locus_tag="OQM87_002794"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCS1508865.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(7943..9145)
                     /gene="pncB"
                     /locus_tag="OQM87_002795"
     CDS             complement(7943..9145)
                     /gene="pncB"
                     /locus_tag="OQM87_002795"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCS1508866.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9411..12026
                     /gene="pepN"
                     /locus_tag="OQM87_002796"
     CDS             9411..12026
                     /gene="pepN"
                     /locus_tag="OQM87_002796"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCS1508867.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12233..13000)
                     /gene="ssuB"
                     /locus_tag="OQM87_002797"
     CDS             complement(12233..13000)
                     /gene="ssuB"
                     /locus_tag="OQM87_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1508868.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(12997..13644)
                     /locus_tag="OQM87_002798"
     CDS             complement(12997..13644)
                     /locus_tag="OQM87_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCS1508869.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNI
                     DRGLVEMARSYGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGI
                     GYLAMNAREFLQTDVVVVAIILYALLGKLADVSAQLLERLWLSWNPAYHLKEATI"
     gene            complement(13656..14801)
                     /gene="ssuD"
                     /locus_tag="OQM87_002799"
     CDS             complement(13656..14801)
                     /gene="ssuD"
                     /locus_tag="OQM87_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCS1508870.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(14798..15757)
                     /gene="ssuA"
                     /locus_tag="OQM87_002800"
     CDS             complement(14798..15757)
                     /gene="ssuA"
                     /locus_tag="OQM87_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCS1508871.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTIGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(15750..16325)
                     /gene="ssuE"
                     /locus_tag="OQM87_002801"
     CDS             complement(15750..16325)
                     /gene="ssuE"
                     /locus_tag="OQM87_002801"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCS1508872.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4092 a         4245 c         4188 g         3864 t
ORIGIN      
        1 ccacggcaat aatggcgggg atccagccat tgcctgaagg tgcggcagaa aaagcccgca
       61 cgcaaatcga atctcaatta cccgcaggtt acaagccggt ttatcttaac cagcttcaac
      121 tgttgtatgc cgcacgcgat atgcaaccca tgtgggaaaa ccgtgatgct gttaaagcct
      181 tccagcaaca gctggcagag gtggcgattg ctggtttcca gccgcagttt aataaatggg
      241 tagagttact gaccgatcct ggtgttaacg ggatggcacg tgacgtggtg ctctctgatg
      301 cgatgatggg ctatctccat ttcattgcaa atattccgat caaaggcact cgctggctat
      361 atagcagtaa accttatgcg ctttcaacgc cgccgctttc ggtgattaac caatggcagc
      421 tagcgctgga taaaggtcaa ttgcctacgt ttgttgcagg actggcaccg cagcatccgc
      481 aatatgcggc gatgcatgaa tcgttactgg ccttactcag tgacaccaaa ccgtggcccc
      541 aactgaccgg caaagcaacg ttgcgcccag ggcagtggag taacgacgta ccggcgttgc
      601 gcgaaatatt gcaacgcaca ggcatgttgg acggggggcc gaaaattact ctacctggcg
      661 atgacacgcc aactgacgcg gtagtcagcc catccgctgt tactgttgaa acagcagaaa
      721 ctaagccgat ggacaagcaa acgacgtctc gtagtaaacc tgcgcctgcc gttcgtgccg
      781 cctacgataa tgaactggtg gaagccgtta aacgttttca ggcatggcaa ggattgggag
      841 cagatggtgc tattggcccg gcaacgcgtg actggttaaa cgtaacgccc gcccagcgtg
      901 ctggcgtgtt ggcactcaac atccagcgat tgcgcttgct gccaacagag ctttctaccg
      961 ggatcatggt taacattcca gcctattcgc tgctctacta tcagaacggc aatcaggtgc
     1021 tggattcgcg agtcattgtc ggtcgccccg atcgcaaaac gccgatgatg agcagtgcac
     1081 ttaacaacgt agtggtaaac ccgccgtgga acgtaccacc aactctggca cgcaaagata
     1141 ttctgccgaa agtgcgcaac gatccgggat atctcgaaag ccatggctat acggtgatgc
     1201 gcggctggaa cagcagagaa gcgattgacc catggcaggt tgactggtct acaatcacgg
     1261 cctcgaattt accgtttcgc ttccagcagg ctccaggccc acggaactcg ctggggcgct
     1321 ataaattcaa tatgccgagt tcagaggcca tttatttgca tgacacgccg aaccacaatc
     1381 tgttcaagcg tgatacacgc gcattgagct caggctgtgt acgagtgaat aaagcttccg
     1441 atctggcgaa tatgctgttg caggatgcag gatggaatga caaacgtatt tctgatgcgc
     1501 tgaagcaggg tgatacacgt tacgtcaata ttcggcagtc gattccggtg aatctctact
     1561 acctgacggc ctttgttggt gcagatgatc gtacccagta tcgtacagat atttacaatt
     1621 atgatctgcc tgcgcgatcc agctcgcaaa tcgtatcgaa agcggaacaa ttaatcaggt
     1681 aaatgaagta gttcggggaa atcaattgtc gtaataatta gcatgattgg gggcgattct
     1741 ctgcagcccc cgtcactgct ggggttgagt catcttgacg tctgctttac gggcggttaa
     1801 ggtgcctctt gtgcgccaga agtgcatata aacgataaca ttgacctgta gacttgatta
     1861 tcatggacaa attcgacgct aatcgccgca aattgctggc gcttggtggc gttgcactcg
     1921 gtgccgccat cctgccgacc cctgcgtttg caacactctc taccccacgc ccgcgcattt
     1981 tgacactcaa taatcttcat accggagagt caatcaaagc ggagtttttc gatggcagag
     2041 gctatattca ggaagaattg gcaaaactta accatttttt ccgcgattac cgcgcgaaca
     2101 aaataaagtc catcgaccca ggattattcg accagttgta tcgcctgcaa gggttgttag
     2161 gcacgcgcaa accggtgcaa ctcatttccg gttatcgttc cattgatacc aacaatgaac
     2221 tacgcgcccg cagccgtgga gtagcgaaga aaagctatca cactaaaggc caggcgatgg
     2281 atttccatat tgaaggtatc gcgttaagca atattcgcaa agccgcgtta tctatgcgcg
     2341 caggtggtgt aggatattac ccacgtagta actttgtgca tattgatacc gggccagcac
     2401 ggcactggta gtaatcgctt aacgaaacag gggcagtatg aactatcgta ttattccggt
     2461 caccgcattc tcccagaact gttcattgat ctggtgtgaa caaacccgcc tggccgcact
     2521 ggtcgatcct ggcggcgatg cggaaaaaat caaacaggaa gttgatgaca gcggcctgac
     2581 actgatgcag atcctgctga cgcatggtca tctggaccac gttggcgcag cggcggaact
     2641 ggcgcaacat tacggcgtgc cggtttttgg cccggaaaaa gaagatgagt tctggctgca
     2701 aggcttgcct gcgcaaagtc gtatgtttgg tctggaagag tgccagtcgc tgacgccaga
     2761 tcgttggctg aacgaaggcg ataccatcag catagggaat gtgactttac aggtgttaca
     2821 ttgccctggg catacgccgg gtcatgtcgt gttttttgat gatcgggcaa agctgctgat
     2881 ttctggcgat gttattttca aaggcggagt agggcgcagt gacttcccgc gtggcgatca
     2941 taatcaactg atttcttcaa tcaaagataa attgctgcca ctgggggatg acgtgacatt
     3001 tattccgggt cacggaccat tatctacact tggttatgag cgcctgcaca atcccttcct
     3061 gcaagacgaa atgcccgtct ggtaaaggcg cataaaaaag tccactttta atgcgggcct
     3121 ggatttctgg caaagtgcgc tttgtttatg ccggatgcgg cacgagcgcc ttatccggcc
     3181 tacaaaatcg tgcaaattca aaatattgca ggggacgcgt aggcctgata agcgtagcgc
     3241 atcaggcaat gttgcgtttg tcatcagtct cagcccgctt ttcagcgggc ttcattgttt
     3301 ttaacgctta cagcactgcc acaatcgatt cgcacagcgg agccatgtta tctggtgtca
     3361 tcccggccac atttacgcga ccagaagcaa cagcatatac gccaaactct tcgcgcagac
     3421 gcagcacttg ttcttttgtc agaccactga aggagaacat gccgttctgt ttgatgataa
     3481 agctgaagtc gcggtttgca cctttttcct gcagcgtatt gacgaacaac tgacgcatac
     3541 gctgaatacg ctggcgcata tcagtcagct cttgttccca aatcgcacgt aatgcatcgt
     3601 tgctcaggat ggtggcaaca acagaagcgc cgtgtgctgg agggttagag tagttagcgc
     3661 gaatcgccgc tttcatttgg ctgaatgcgc gatcaacgat ttcactgtcg gcagcaacca
     3721 gagtacaagc gccaacacgc tcgttgtaca ggccaaagtt tttagagtag gaactggcaa
     3781 caatcagctc tttatgcata gccgagaaag cgcgcagtcc ttcagcatct tcttccagac
     3841 cacgggcaaa accctggtaa gcgaagtcaa acagcggtaa ccagcctttc tcaacggaga
     3901 gttgtgccag tgtctgccat tgttccagcg tagggtcgat accggttggg ttatggcagc
     3961 agccatggaa cagcactacg tcgccagcct gagcttcgtt cagactgtta atcagtgcat
     4021 cgaagtcaag ggtgtgattt tccgcatcat aataagcgta ttcacgaact tccagacctg
     4081 cagagttaaa gacgctctta tggttcggcc agcttgggtt gctcacccac acacgcttaa
     4141 cgctggtatt ttttgccagg aagtcggcag ccacgcgtag tgcgccagtg ccacccggag
     4201 tctgtgccgt gcgagcacgt ttgtcattga tcagggcgct acctttacca aacagcagtt
     4261 cctgagtgca gcgaccaaat tcagggatgc cgtcaatgcc gaggtaattt ttggtggttt
     4321 cattttcgag cagatactgt tcagcctttt tcacgctggt cagtaccggg gttttgcccg
     4381 tctcatcttt atagacacca atcccgaggt taattttgcc gggacgttca tcggcacgaa
     4441 acagatcggc caggcccaga atcgggtcgg caggagcggc ggtaatgttc tcaaacatga
     4501 cgaggttcca ttatggttac agaagggaag tccgctatca gggtaacggg agatttacaa
     4561 aattccaatt attactgatg aaaacgcagg ctgtttttgc aagacgtgag attgctctgg
     4621 aaggcataaa aaaaacagga ccaacgtcct gttttttcgg catttaacaa agaggtgtgc
     4681 tattagaact ggtaaacgat acccacagca acggtgtcgt ctgaacctac gcccagtttg
     4741 ttgtcagaat cgatctggtt gatgatgtag tcaacatagg tggacatgtt tttgttgaag
     4801 tagtaggttg cgcccacttc aaagtagttc accagatcaa catcaccgat accttctacg
     4861 tctttcgctt tagatttggt gtaagcgatg gacggacgca gaccgaaatc gaactggtat
     4921 tgcgcaacta acagaacgtc ttgcgttttg ttggcgaagc cgctggtgtt tgtaaattta
     4981 ttagtgatcg gcgtagcgtt acgggtttca ccgtagttcg ctgccaggta gatgttgttc
     5041 gcgtcgtact tcagaccagt agcccactgt tcagcttttt taccgttgcc aagaggttga
     5101 gcttcttgca ggttggtacg gtcagctgca ccataagcac caacgatacc aaagccttcg
     5161 tattcgtagc tgatagaacc gccaacaccg tcgccgttag aacggcgtgc agtgtcacgc
     5221 tcgtttttac ccaggtactg aacagcgaag ttcaggccat caaccagacc aaagaagttg
     5281 gagttacgat aggtagcaac gccgccaaca cgaccaacga agaagtcatc gctgtatgca
     5341 gtatcaccac caaattctgg cagcatatcg gtgtaaccca gtgcatcata aaccacaccg
     5401 tagttacggc cgtaatcgaa agaaccaacg tcagcgtatt taagacccgc gaatgccaga
     5461 cgcgttttgt taccagtttg agcgtcagcg ccttcagagt tgttaccctg gaagttatat
     5521 tcccactgac cataaccggt cagatcggaa ttgatttgag tttccccttt aaaaccaaga
     5581 cgggcatagg tcatgtcgcc attgccaccg taactgtttt caccgttacc cttggaaaaa
     5641 taatgcagac caacagcttt accgtacaga tctactttgt tgccatcttt gttatagatt
     5701 tctgcagcgt ttgcagtacc tgctactaac agagcaggga cgatcactgc cagaatattg
     5761 cgcttcatca ttatttatta ccctcatggt tttttttatg acacctgcca ctgccgtcaa
     5821 taagttctgt caataaaaat ttacggaact attgatgaga gtttggtgtc tttatgtgtc
     5881 tgcaggcatc tttccattca aactaacgtt tcgctaccgt gaaagtgcta caaagataaa
     5941 gatttagttt caaaaagaaa aaatatgtaa ccaaaagtaa aaattaagga gctttgtgaa
     6001 caccgtcata tttccataga aacgtgatga tatttacagc aatttttatc tatttatatg
     6061 atttacttat atttaaatta actaaacgga aattttgttt ctgatggaaa ctttatcgac
     6121 ctggcacaaa atcctctttc agacatccag aatgccaaaa aatagtatga aaattgtgct
     6181 atcagctaag aaaaaactaa tccgcaataa gaatgcgggt tattttttgt gcgtaaatgt
     6241 tgcaatgtaa tattccgtaa caggatgatc gttatggcgg gggtgcaaat agattgcttg
     6301 acttaacaaa cagataactt gacagaaagg ataaaaaaga gccagcaggc gctggctctt
     6361 tgaggaagaa aatcagaagc tggcgttgcg cggagtacgt gggaacggaa tcacatcacg
     6421 cacgttttgc acgccagtta cgtaagcaat caggcgttca aaaccaagac cgaaacctga
     6481 atgcggaaca gtaccgtagc gacgcagatc gcgataccac cagtaatctt ctttattcag
     6541 gcccatttcc agcatacgct cgtccagaac gtccagacgt tcttcacgct gggagccacc
     6601 aatgatctca ccgatgcccg gagccagaac gtccatagcc gcaacggttt taccgtcttc
     6661 gttgaggcgc atatagaacg ctttaatatc tttcggatag tttttaacca ctaccggtgc
     6721 tttaaagtgt tcttccgcca gataacgctc atgctcagaa gagagatcga cgccccagta
     6781 aaccgggttt tcaaacttcc tgccgcagtt ttcgagaatg gtcactgcgt cggtgtaatc
     6841 cacctgtgcg aaatcggctt caatgaagcg ttccagacgt gaaacggcat ctttatctac
     6901 gcgttcagcg aagaatttca tgtcgtctgc gcgttcttcg agaaccgctt tgaagacata
     6961 tttcagcatg gcttcagcca gacccgcgat atcgttcagg ttagcaaacg ccacttccgg
     7021 ctccagcatc cagaattccg ccaggtgacg gctggtgttg gagttttcag cacggaaagt
     7081 cgggccgaag gtataaattt tggataatgc gcaagcgtag gtttcgccgt tcaactggcc
     7141 agatacggtc aggaaagact ctttaccaaa gaagtctttg tcgaaatcca ctttgccctg
     7201 atcgttacgc ggcaggtttt ccagatccag cgtagaaacg cggaacattt cgcctgcacc
     7261 ttcggtatca gatgcggtaa tcagtggcgt tgaaacccag aagaatccct gctcgttaaa
     7321 gaagcgatgc agcgcctgcg ccagcgtatg gcgaacgcgc gcgacggcac caatcaggtt
     7381 tgtgcgcgga cgcaggtgag cgacttcacg cagatactca atgctgtggc gttttgccgc
     7441 catcgggtaa gtgtctggat cttcaaccca accagcaact tcaaccttgc tggcctgaat
     7501 ttcaaattgt tgcccctggc ccggcgatgc cacgacttta cccgtcacaa tgaccgagca
     7561 gccggtggtc agacgcagga cgtcttcatt gtaattgggc agagaattat tgatgacagc
     7621 ctgtacagga tcaaagcagg aaccgtcata aacggcgagg aaggagatgc cagcttttga
     7681 atctcggcgg gtacgtaccc atccgcgtac ggtgacttcg ctgtcaacgg ctacgcggcc
     7741 ctggagtacg tcggctacag gcacaacgct cataatattc tctctgttaa tagtcggaaa
     7801 aaataaacac ttgtccaccc gtaaatgggg ggggatacct atgttacctg gcatctgcaa
     7861 tcagacaagc agaattcgca aatgcagcga aagatttcgg aattaaaggt aaaaaaggga
     7921 gccgattagc tccctgatga tattaactgg cttttttaat atgcggaagg tcgaacgctt
     7981 tgcgcagcgc ccgaacaaac gctttatcat ggcagatagt tttgccaggg ctgtcagaaa
     8041 gtttcgccac cggtttaccg ttacactcta ccaacttaat cacaatattc aggggtttta
     8101 cctgggggat atcgcaggtc aggcgagtcc caataccaaa acttaattgc acgcgggaag
     8161 agaagtggcg gtatagctca accgctttgc gtaaatccag attgtcagag aaaaccagcg
     8221 ttttactctg tggatcaatt cccagctttt cataatgtgc aatggctttt tcaccccatt
     8281 caaccgggtc gccagagtca tgacgcaggc cctgataccg actagcgaac tcgacaccga
     8341 aatcacgcag gaaagcatcc atagtgatgc agtcggttaa tgcaatgcca agttggtcgg
     8401 gatactcttc cagccaggca gcaagtgcag ctcgctggct gttggctaga tccgggctga
     8461 tttgctgatg tgcctggaac cattcgtgtg cctgtgttcc catcggcgtg agggaaagcc
     8521 gacgcgccag atcgtagttg ctggtgccca cgaaccagga ttcctgttgc agacgcttaa
     8581 cgatggtttc ttgtacttcg cgagaaaaac ggcgacgggt gccaaaatcc atcagatgga
     8641 agcgcgacat atcaagaccg gcggttaacg ccgagaagtc gactaatttg ctttccagcg
     8701 tgtcgagggc ttgcgcaacg tcggcctgcg gtgagcgata gcgatgtacc atttcactga
     8761 taaccgccag caaaggaact tcccagagga tgacttcacg ccacggaccg cttaagcgaa
     8821 tatccagctt gccattatcg ttggacacgg tgacttgttc cgggttaaag cggaactcgc
     8881 gtaaccagtt aagatagtcg gccttaaaga aaggcagggc agagagccac tgatattcat
     8941 catcctgcag gcgcaggtgc tgcatcgcct gaatctgttc acgaatagca tcggcataaa
     9001 tacccagcag atcgtcacct cggcaacgaa actccgccgc gacatgcaca tcgtaatagt
     9061 gatgaaacac ggcttgctgc atatgcaact tataagcatc tgtatccagc aacgagtgca
     9121 gaacaggaga agcgaattgt gtcataggtg cgcagtagcg tcctcgtaca ggagcgttta
     9181 gtacaataaa catcttcagg aactgctgga gtataccttg tttcgcaatt tattgaaccc
     9241 cgatcacacc atatgccacc tttctggtcg atggcattac gcggtgtatg ttataaaaat
     9301 gtagcaataa aggcgtttgt acctgaaaag atgaagattc tgcatagcgc gatttacgca
     9361 acaggaatag actgaacacc agactctata aaagatgcta aaggttattt atgactcaac
     9421 agccacaagc caaataccgt cacgattatc gtgcgccgga ttaccagatt actgatattg
     9481 acttgacctt tgacctcgac gcgcaaaaga cggtcgttac cgcggtcagc caggctgtcc
     9541 gtcatggtgc atcagatgct ccgcttcgtc tcaacggcga agacctcaaa ctggtttctg
     9601 ttcatattaa tgatgagccg tggaccgcct ggaaagaaga agaagagggc gcactggtca
     9661 tcagtaattt gccggagcgt tttacgctta agatcattaa tgaaataagc ccggcggcga
     9721 atacggcgct ggaagggctt tatcagtcag gcgatgcgct ttgcacccag tgtgaagccg
     9781 aaggtttccg ccatattacg tattatctcg accgcccgga cgtgctggcg cgttttacca
     9841 ccaaaattat tgccgataaa atcaaatatc ccttcctgct ttccaatggt aaccgcgttg
     9901 cgcaaggcga actcgaaaac ggacgccatt gggtacagtg gcaggacccg ttcccgaaac
     9961 cgtgctacct gtttgcgctg gtggcaggcg actttgatgt actgcgcgat acctttacca
    10021 cgcgttctgg tcgcgaagta gcactggagc tgtacgtcga tcgcggcaac cttgatcgcg
    10081 cgccgtgggc gatgacctcg ctgaaaaact ccatgaaatg ggatgaagaa cgctttggcc
    10141 tggagtatga cctcgacatc tatatgatcg tcgcggtgga tttcttcaat atgggcgcaa
    10201 tggagaataa gggtctgaat atctttaact ccaaatatgt gctggcccgc accgacaccg
    10261 ccaccgacaa agattacctc gatattgaac gcgttatcgg ccatgaatat ttccataact
    10321 ggaccggtaa ccgagtgacc tgccgcgact ggttccagct cagcctgaaa gaaggtttaa
    10381 ccgtcttccg cgatcaggaa ttcagctctg accttggttc ccgcgcagtt aaccgcatca
    10441 ataatgtgcg caccatgcgc ggattgcagt ttgcggaaga cgccagcccg atggcgcacc
    10501 caatccgccc ggacatggtc attgagatga acaacttcta caccctgacc gtttacgaga
    10561 agggcgcgga agtgatccgt atgatccaca ccctgctggg cgaagaaaac ttccagaaag
    10621 ggatgcaact ctatttcgag cgtcatgatg gtagcgcagc gacctgtgac gactttgtgc
    10681 aggcgatgga agatgcgtcg aatgttgatc tctcccattt ccgccgttgg tacagccagt
    10741 ccggtacgcc ggttgtgacc gtcaaagacg actacaatcc ggaaactgag cagtacaccc
    10801 tgaccatcag ccagcgcacg ccagccacgc cggatcaggc agaaaaacag ccgctgcata
    10861 ttccgtttgc catcgaattg tatgacaacg aaggcaaagt gatcccgttg cagaaaggcg
    10921 gtcatccggt gaattccgtg ctgaacgtca cccaggcgga acagaccttt gtttttgata
    10981 atgtctactt ccagccggtg cctgcgctgc tgtgcgaatt ctctgcgcca gtgaaactgg
    11041 aatataagtg gagcgatcag caactgacct tcctgatgcg tcatgcgcgt aatgatttct
    11101 cccgttggga tgcggcgcaa agtttgctgg caacctacat caagctgaac gtcgcccgtc
    11161 atcagcaagg gcagccgctg tctctgccgg tacatgtggc tgacgctttc cgcgcggtgc
    11221 tgctcgatga gaagattgat ccggcgctgg cggcagaaat cctgacgctg ccttctgtca
    11281 atgaaatggc tgaactgttc gatatcatag acccgattgc tattgccgaa gtacgcgaag
    11341 cactcactcg tactctggcg actgaactgg cggatgagct gctggctatt tacaacgcga
    11401 attaccagag cgagtaccgt gttgagcatg aagatattgc aaaacgcact ctgcgtaatg
    11461 cctgcctgcg cttccttgct tttggtgaaa cgcatctggc tgacgtgctg gtgagcaagc
    11521 aataccatga agcaaacaat atgaccgatg cgctggcagc gctttctgcg gcggttgctg
    11581 cacagctgcc ttgccgtgac gcgctgatgc aggagtacga cgataagtgg catcaggacg
    11641 gtctggtgat ggataaatgg tttatcctgc aagccaccag cccggcggcg aatgtgctgg
    11701 agacggtgcg tggtctgttg cagcatcgct catttaccat gagcaacccg aaccgtattc
    11761 gttcgttgat tggtgcgttt gcgggcagca acccggcagc gttccatgcc gaagatggca
    11821 gcggttacca gttcctggtg gaaatgctta ccgacctcaa cagccgtaac ccgcaggtag
    11881 cttcacgtct gattgaaccg ctgattcgcc tgaaacgtta tgatgccaaa cgtcaggaga
    11941 aaatgcgcgc ggcgctggaa cagttgaaag ggctggaaaa tctctctggc gatctttacg
    12001 agaagataac taaagcactg gcttgatact tttgagccaa aatgcctgat gcgacgcttg
    12061 acgcgtctta tcaggcctac aactccgttg aaattgctgg ttttgtaggc tggataaggc
    12121 gttcacgccg catccggcag ccgtgcccaa ctgcctgatg cgatgcttga cgtgtcttat
    12181 caggcctaca agtccgttga aaatgctggt tttgtgcgcc acatccggca tattaaccat
    12241 gacgccgaat cagttgttca gaatgcccac gccgcattac ccgttgcaat acttccgctt
    12301 ccagctccgc taatcgcacc gaccctaatc gacgcggacg ggggatatcc actgtcaaat
    12361 ccagactaat ttttccctct tcaattaaca acacccggtc agccattgct accgcttcgc
    12421 tcacatcatg cgtcaccaac agtacggtaa agccgtgctg ctgccaaagt gacacaatca
    12481 aatcctgcat ctcgagtcgc gttaaggcgt ccagtgcccc gagcggttca tcaagcaaca
    12541 ataaacccgg tcgatgaatc aacgctcttg ccagcgccac acgctgtttc tgcccgccag
    12601 aaagtgcggc aggccattcc ccggcgcgat tctccagtcc taccgcagcc aacgcccgac
    12661 gcgcggcatc gcgccactga cctttaaggc ctaacccaac gttatcaatc accgatttcc
    12721 acggcagcag acgcgcatcc tgaaacatca ttcgcgtatc atcctgaatt tcagccagcg
    12781 gtgtggtgcc cgctaacaca tcgcctgcgg ttggcgtttc cagaccggcc agcaggcgca
    12841 gcagggtact tttaccacca ccgctgcggc ccaccaccgc cacaaactga cctgccggaa
    12901 tatgtaaatc cagttggttc aggacgatat tttccgcgta atgtttgctt actgcattga
    12961 gcaacaatgg tgtgccctgg ttcagacgag cagtattcat atcgtggcct ccttcaaatg
    13021 ataagccggg ttccagctca accagaggcg ctctaacagc tgcgcgctga cgtcagcaag
    13081 tttgccgagc agggcgtaaa gaataatagc gactaccacc acgtctgttt gcaaaaactc
    13141 tcgcgcattc atcgccagat aaccaatgcc ggaattggca gaaatggttt cggcaacaat
    13201 cagcgtcagc cacatcaggc ccaacgcaaa acgcacgccg accataattg agggcagggc
    13261 atcgggcagg atcacatgga taaacagcgg tatgccggat aatccatagc tacgcgccat
    13321 ctccaccagc ccgcgatcga tattacggat gccatgccag gtgttgatat aaatggggaa
    13381 cagcgtaccg agcgccacca gaaagatttt cgcggattca tcaatgccaa accacaaaat
    13441 caccagcggg atcagcgcca gatgctgcca cagttcgccg ctggcggaga gcgtccagaa
    13501 cgccgtcacc actccttccg gtgaaggcaa aatacgcgtc gacagccagc caaccgagga
    13561 ggccagttgc cacaccgcca cgatgcccac cggtaaaaac cagggggcaa cgcgcaataa
    13621 ccacttcttc actggcgttg ccatcatcgt gttccttagc tttgcgcgac tttacggggg
    13681 ataaaatcat tcgccaccgc ttcgccttgc ggattcagcg gctgcggctg gggaatttcc
    13741 ggaatggcga catccagatg tgggaacagc aactcgccaa cccgatacgc ttcttccaga
    13801 tgcgggtagc ccgaaagcac aaagctgtcg atgccaagcg cggcgtattc gttgattcgc
    13861 gcggcgaccg taggaccatc gcccaccagc gccgttccgg caccgccgcg cactaagcca
    13921 acgcccgccc ataaattggg gctgatctcc agattgtcgc gcttgccgtt atgtagcgcc
    13981 gccattcgct gttgtcctac ggaatccgtc cgggcgaatg cggcttgtgc tttggcgata
    14041 gtctcatcat caagatgcga gattaaccgc tcggcggcct gccacgcttc gtcgttagtt
    14101 tcacgaacaa tcacatgcag acgaataccg aaacgaattt tgcgcccata cgcggcagct
    14161 ttcgcccgca cttgttcgat tttctcttta acaagttccg gcggttcgcc ccaggtgagg
    14221 tagagatcaa cttgttctgc cgccagctcc tgggcgacat ctgacgatcc gccaaagtaa
    14281 agcggaggat aaggctgttg aatcggcggg aagagcagtt ttgctccgcg cacatgaata
    14341 tgtttaccgt tgaaattgac ggtttccccc agcaataagc gccgccagac ctgggtaaat
    14401 tccgccgagg cttcgtagtg ctcgctatga tcaaggaaca cgccgtcgcc tgccagctct
    14461 tgtggatcgc tgcctgtgac cagattaaac aacgcacgac catttgagag acggtcaagc
    14521 gtggcggcct ggcgggcggc aacagtaggt gaggttacgc tgggacgcag ggcgacaaga
    14581 aacttcagcc gctgcgtcac cgggatcatc gacgctgcaa ccagccacgc atcttcacac
    14641 gatcgccccg ttgggatcag cacaccggta tagcccagac gatccgccgc ttgcgcaatt
    14701 tgttgcagat aaccgtgatc aaccgggcgt gaaccttctt ccgttcccag atagtgcccg
    14761 tcaccgtggg tcggtaaaaa ccagaacata ttcagactca taattgtttt ccttccagtt
    14821 gagtgggctg ccagatgcgc tggcgaatat cgactttttt cggcaccaga cggttttcat
    14881 aaaacagatc tgccgtttgc tgctgtaagg cggcaacctc ggcgttaacc ggtttgatgg
    14941 tggtaggagg gcgatgatct aagtaagagg caatcaccgg tgccggtaag ccaatcgttt
    15001 ttgccagtaa agcgatgctt tgttcgcgct ggctgcgggt taacgcatcg gcctcactaa
    15061 aggttgccag tacgccctga ataaaagcgc cgtttttttc tgcataggga cgagctgcca
    15121 gataaaacga tccagtttga ttgagatcgg tgccgtcttt cagcacccgc acgccgccct
    15181 gtaataacgc agcggagtag tagggatccc agatagccca ggcgtcaacg ttaccttgct
    15241 ggaacgcggc gcgggcaccg gcaggcgtca ggtaagtggg ctggatatcg gtgaatttaa
    15301 gtccggcctg acgcagtgca cgcagtaaaa ggttgtgtga actggaacct ttctgaaagg
    15361 caactttgtg acctttaaga tcggctacgg ttttgatcgg gctgttttct gccaccagaa
    15421 tcacttcggc tttgggcttc ggtggctcga cgcccacata taccaaatca gccccggcag
    15481 cctgggcaaa gattggcgga atatccccgg tactgccgag atcaatactg ccaacgttta
    15541 acgcttccaa catttgcgga cccgcgggga actccaccca ggagattttt gattgcggat
    15601 agcgtttttc cagtaactgg tggctttttg ccagcaccat accaatactg cctttctgat
    15661 agcctatacg taacgcttca ggcgaggatt ctgcagcaac cgcaaaagta gagacgctaa
    15721 gcaatcccgc cagcgccagt ttaatgatgt tacgcatggg cattacctcg cagagacaga
    15781 aggtcaggaa cctgaacatc gcggcggtgc aatgcctgcc agaaagtttc tagcgcggta
    15841 tcaagatggg gttgcagatt tggcgtgaac tggggtctgt gatggtaatc aattaattgt
    15901 gagtcatcgg caaacacgcc gtgcaggatc tcctgagctt tcagtgcgct taaaactggt
    15961 ttaagggcat aatcgaccgc cagcagatgg gccacggtac cgcccgtcgc cagcggtagc
    16021 accactttgc cttgcaaagc gcgctctggc agcaggtcga gcagggtttt cagcgcgccg
    16081 gaataggcgg ctttatacac aggcgtggcg acaatcagcc catcagcctg ttgcagctgt
    16141 tcggtgaagg tcttgagtgc cggactatcg aaacgagcat aaagcagatc ttccggggcg
    16201 aagttttgca gattccagtg ataaacctct acatctaggc catttagttt ttcccgcgca
    16261 tattccagca aggagctgga gcgagaagga aagcgaggac tacccgccag ggtgatgaca
    16321 cgcatactct ctccttataa ccaattgttc ttttttttgt taacattgat aacaattcgg
    16381 tcagtctgt
//
LOCUS       DAJVQH010000086        16224 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJVQH010000086 DAJVQH010000000
VERSION     DAJVQH010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.086"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..1963)
                     /gene="speA"
                     /locus_tag="OQM87_002802"
     CDS             complement(<1..1963)
                     /gene="speA"
                     /locus_tag="OQM87_002802"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCS1508873.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYEVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTY"
     gene            complement(1972..2103)
                     /gene="yqgB"
                     /locus_tag="OQM87_002803"
     CDS             complement(1972..2103)
                     /gene="yqgB"
                     /locus_tag="OQM87_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCS1508874.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2239..2454
                     /locus_tag="OQM87_002804"
     CDS             2239..2454
                     /locus_tag="OQM87_002804"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508875.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2758..3912
                     /gene="metK"
                     /locus_tag="OQM87_002805"
     CDS             2758..3912
                     /gene="metK"
                     /locus_tag="OQM87_002805"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCS1508876.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4349..5743
                     /gene="galP"
                     /locus_tag="OQM87_002806"
     CDS             4349..5743
                     /gene="galP"
                     /locus_tag="OQM87_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCS1508877.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWRTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5820..6317
                     /locus_tag="OQM87_002807"
     CDS             5820..6317
                     /locus_tag="OQM87_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCS1508878.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6412..7119
                     /gene="endA"
                     /locus_tag="OQM87_002808"
     CDS             6412..7119
                     /gene="endA"
                     /locus_tag="OQM87_002808"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCS1508879.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7199..7930
                     /gene="rsmE"
                     /locus_tag="OQM87_002809"
     CDS             7199..7930
                     /gene="rsmE"
                     /locus_tag="OQM87_002809"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCS1508880.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            7943..8893
                     /gene="gshB"
                     /locus_tag="OQM87_002810"
     CDS             7943..8893
                     /gene="gshB"
                     /locus_tag="OQM87_002810"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCS1508881.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9002..9565
                     /locus_tag="OQM87_002811"
     CDS             9002..9565
                     /locus_tag="OQM87_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCS1508882.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9565..9981
                     /gene="ruvX"
                     /locus_tag="OQM87_002812"
     CDS             9565..9981
                     /gene="ruvX"
                     /locus_tag="OQM87_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCS1508883.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10157..11137)
                     /locus_tag="OQM87_002813"
     CDS             complement(10157..11137)
                     /locus_tag="OQM87_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCS1508884.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11155..11859
                     /gene="yggS"
                     /locus_tag="OQM87_002814"
     CDS             11155..11859
                     /gene="yggS"
                     /locus_tag="OQM87_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCS1508885.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11877..12443
                     /gene="yggT"
                     /locus_tag="OQM87_002815"
     CDS             11877..12443
                     /gene="yggT"
                     /locus_tag="OQM87_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCS1508886.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12440..12730
                     /locus_tag="OQM87_002816"
     CDS             12440..12730
                     /locus_tag="OQM87_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCS1508887.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANNHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12738..13331
                     /locus_tag="OQM87_002817"
     CDS             12738..13331
                     /locus_tag="OQM87_002817"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCS1508888.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITHEPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13324..14460
                     /gene="hemW"
                     /locus_tag="OQM87_002818"
     CDS             13324..14460
                     /gene="hemW"
                     /locus_tag="OQM87_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCS1508889.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14774..15737
                     /locus_tag="OQM87_002819"
                     /pseudo
     CDS             14774..15737
                     /locus_tag="OQM87_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15782..>16224
                     /locus_tag="OQM87_002820"
     CDS             15782..>16224
                     /locus_tag="OQM87_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCS1508890.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYA"
BASE COUNT         4001 a         4192 c         4091 g         3940 t
ORIGIN      
        1 gataagtata accgtacaaa cctgcctcga actcttcaag gaactgttgt tgcagttcag
       61 catcaagatc ggttttcttc acttgatcgc ggaactgggt taacagcgtt ttcggatcga
      121 gctgtacata ttgcagcatg tccgccacgg tatcgccttc gtcagacagt tctacttcta
      181 cgctaccgtc agggaacacg aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca
      241 tattgccaag gatctcctga tatgcaccga ccataaagaa accgagcatc ggcggattct
      301 ctggatcgta ctccggcatt ggcattgtcg tggcaatacc atcaccatca atatagtggt
      361 cgatagcacc gtcagagtca caggtaatat ccagcagcac agcgcgacgt tccggcactt
      421 gatccagccc ttccagcggt agaaccggga acaactggtc gatcccccat gcgtccggca
      481 ttgactggaa cagcgagaaa ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt
      541 cgattatcgg acgatgcgca cggttttgcg gatccagttg cttctgcact tcatggcaca
      601 tgctcagata gagttgttct gcccatgcac gttcttgcag gctaaaggtg ccggaagagt
      661 agccgatatg aatgtcgtgc agatccatct ggctgtcgtg taaccattca cgcaaagaac
      721 ggcgagttcc cggttcgtgc atttcctgcc aggtttccca catgctttgc agcgcacgcg
      781 gcgcatcttc tgcgggcgcg gtcggcaccg tatattcgtt acgttccacg ccgatgatat
      841 tcgacaccag cacggtatgg tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg
      901 gatgcggcag accattttct tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt
      961 attcattgag gccatagttt acggagcagt cagactgcga acgggtgcct tcataatcca
     1021 cgcccagacc gccgccgacg tcgaagcact gaatattgac gcccagcttg tgcagttcta
     1081 catagaaacg cgcggattca cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg
     1141 aaccgaggtg gaagtgcagt agttgcaggc tgtcgagacg cccggcttca cgcagggttt
     1201 caaccagttg cagtacctga gtcgcagcca ggccgaactt cgatttttcc ccgccggagg
     1261 actgccattt acccgagccc tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga
     1321 cattcagacg ttctgcttca tccagcacaa tggcgatttc tgacatcttc tcaatgacca
     1381 gatagacctt gtgccccatc ttctcgccaa ttaatgccag gcggatatat tcgcggtctt
     1441 tataaccgtt gcagacgatg acgctacgag tcatgccagc atgtgccagc actgccatca
     1501 actcggcttt ggaaccggct tccagaccca gcggttcacc cgaatgaatc agggactcga
     1561 tcacgcggcg gtgctggtta actttgatcg gataaacaag gaagtaatcg ccgttatagc
     1621 cgtaggattc cctcgcacgt ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga
     1681 tctgtgggaa acagaacagt gcaggcagac gctgaccctg tgcttcacga gttttcacta
     1741 actgcgcgag atcgacgcga gcttccggga cgtccgggtc cgggcacacg ctgatgtggc
     1801 ccagctcgtt aacctcatag tagttattgc cccaccaggc aatattgtaa gtacgcagca
     1861 tcttgctggc ttcctgggag ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt
     1921 cgcccgctga cgaaggcaaa cccatagaca tgtcgtcaga catagcgaac ctcaaattat
     1981 tttattaagt gtaaaacagt taacgactgt cgcagcctgg aactgcgata acaacccata
     2041 agcacatgga ttttccagca gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt
     2101 cataacatta ttaagcacat aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa
     2161 aactcttgtt tttacaagag cgcccttgtt cagtcctcag taactgtaac cagctcttga
     2221 atcctgagaa gcgccgagat gggtataaca tcggcaggta tgcaaagcag agatgcagag
     2281 tgcggggaac gaatcttcac cagaacggtg agacaggtta agcagcagac aacggttcat
     2341 tatttcgtat cacctccacg gccgcctgtt aagacgaacc cacaagccaa aactctgatt
     2401 tcaacccggc tggaagtggc aacacgaaaa aaacgtcgtg tgctttttat ttaagccgcg
     2461 cgccgcgttt tataccccac aatggcagaa aattgcaaaa gataaatacg cagaatgccg
     2521 gcattgtcag gaaaaatttc cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa
     2581 agtggaaatc gggtgaagaa ttgacctaaa atagccatcc agatgttaat ccatccatac
     2641 cgattaacac tcagactgcc agtgttttta acctgcagag tcgtggtagg atccgctacc
     2701 acagaaaatc cacacaacag tttgagctaa ccaaattctc tttaggtgat attaaatatg
     2761 gcaaaacacc tttttacgtc cgagtccgtc tctgaagggc atcctgacaa aattgctgac
     2821 caaatctctg atgccgtttt agacgcgatc ctcgaacagg atccgaaagc acgcgttgct
     2881 tgcgaaacct acgtaaaaac cggcatggtt ttagttggcg gcgaaatcac caccagcgcc
     2941 tgggtagaca tcgaagagat cacccgtaac accgttcgcg aaattggcta tgtgcattcc
     3001 gacatgggct ttgacgctaa ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct
     3061 gacatcaacc agggcgttga ccgtgccgat ccgctggaac agggcgcggg tgaccagggt
     3121 ctgatgtttg gctacgcaac taatgaaacc gacgtgctga tgccagcacc tatcacctat
     3181 gcacaccgtc tggtacagcg tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg
     3241 cgcccggacg cgaaaagcca ggtgactttc cagtatgacg acggcaaaat cgttggtatt
     3301 gatgctgtcg tgctttccac tcagcactct gaagagatcg accagaaatc gctgcaagaa
     3361 gcggtaatgg aagagatcat caagccaatt ctgcccgctg aatggctgac ttctgccacc
     3421 aaattcttca tcaacccgac cggtcgtttc gttatcggtg gcccaatggg tgactgcggt
     3481 ctgactggtc gtaaaattat cgttgatacc tacggcggca tggcgcgtca cggtggcggt
     3541 gcattctctg gtaaagatcc atcaaaagtg gaccgttccg cagcctacgc agcacgttat
     3601 gtcgcgaaaa acatcgttgc tgctggcctg gccgatcgtt gtgaaattca ggtttcctac
     3661 gcaatcggcg tggctgaacc gacctccatc atggtagaaa ctttcggtac tgagaaagtg
     3721 ccttctgaac aactgactct gctggtacgt gagttcttcg acctgcgccc atacggtctg
     3781 attcagatgc tggatctgct gcacccgatc tacaaagaaa ccgcagcata cggtcacttt
     3841 ggtcgtgaac atttcccgtg ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc
     3901 ggcctgaagt aatctttctt cacctgcgtt caaaggccag cctcgcgctg gcctttttct
     3961 tttacctcac cgcggtggat aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca
     4021 ataacatcat tcttcctgat cacgtttcac ggcaaattac catcacaatt gaaaccgatt
     4081 acaccaacca caccagcaaa atatttgtaa tattttcata ttattattca gctttcacag
     4141 ttgttacatt tcttttcagt aaagtcttaa ttgcagatga cagcgtttaa tctatgatga
     4201 tataactcaa ttattttcat gcacttaaat cataactaag ataaatgtta gtgtaagcga
     4261 ttacactgat gtgatttgct tcacatcttt ttacgtcgta ctcacctatc ttaattcaca
     4321 ataaaaaata accatattgg agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa
     4381 caaggcaatg acgtttttcg tctgcttcct tgccgctctg gcgggattac tctttggcct
     4441 ggatatcggt gtaattgctg gcgcactgcc gtttattgca gatgaattcc agataacttc
     4501 gcacacgcaa gaatgggtcg taagctccat gatgttcggt gcggcagtcg gtgcggtggg
     4561 catcggctgg ctctccttta aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct
     4621 gtttgttgcc ggttcactgt tctctgcggc tgcgccaaac gttgaagtac tgattctttc
     4681 gcgcgttctg ctggggctgg cggtgggtgt ggcctcttat accgcaccac tgtacctgtc
     4741 agaaattgcg ccggaaaaaa ttcgcggcag tatgatctcg atgtatcagt tgatgatcac
     4801 tatcgggatc ctcggtgctt atctttctga taccgccttc agctacaccg gtgcatggcg
     4861 ctggatgctg ggtgtgatta tcatcccggc aattttgctg ctgattggtg tcttcttcct
     4921 gccagacagc ccacgttggt ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct
     4981 gctacgcctg cgtgacacca gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag
     5041 tttgcaggtt aaacagagtg gctgggcgct gtttaaagag aatagcaact tccgccgcgc
     5101 ggtgttcctt ggcgtactgt tacaggtaat gcagcaattc accgggatga acgtcatcat
     5161 gtattacgcg ccgaaaatct tcgaactggc gggttatacc aacaccaccg agcaaatgtg
     5221 gaggacagtg attgtcggcc tgaccaacgt acttgccacc tttatcgcaa tcggccttgt
     5281 tgaccgctgg ggacgtaaac caacgctaac gctgggcttc ctggtgatgg ctgctggcat
     5341 gggcgtactc ggtacaatga tgcatatcgg tatccactct ccgtcggcgc agtatttcgc
     5401 catcgccatg ctgctgatgt ttattgtcgg ttttgccatg agtgccggtc cgctgatttg
     5461 ggtactgtgc tccgaaattc agccgctgaa aggccgcgat tttggcatca cctgctccac
     5521 cgccaccaac tggattgcca acatgatcgt tggcgcaacg ttcctgacca tgctcaacac
     5581 gctgggtaac gccaacacct tctgggtgta tgcggctctg aacgtactgt ttatcctgct
     5641 gacattgtgg ctggtaccgg aaaccaaaca cgtttcgctg gaacatattg aacgtaatct
     5701 gatgaaaggt cgtaaactgc gcgaaatcgg cgctcacgat taatctcctc aggcttcctc
     5761 ccgtctcgga ggaagccgcc tcttgcagtc atcttttctt cgctctatcc tctgccgcta
     5821 tgaaaacatc ccgtctccct atagccatcc aacaggccgt tatgcgccgc ctgcgggaaa
     5881 aactcgccca ggccaacctg aagctagggc gtaactaccc ggagccaaaa ctctcttaca
     5941 cccagcgcgg aacctccgcc ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc
     6001 ccgttttgct gttggaaaac agtgaagctt ttattgaaga agtggtaccg cacgaactgg
     6061 cacatttgct ggtatggaaa catttcggcc gcgtagcgcc acatggcaaa gagtggaagt
     6121 ggatgatgga aagcgtgctg ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat
     6181 ccgtgcgtcg caacaccttc ccctaccgct gtaagtgcca ggaacatcag cttaccgtac
     6241 gccgccataa tcgcgtagtt cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac
     6301 agctggttgc gaaataacca cctgaactat caggaacttt cctgatctgg ctgattgcat
     6361 accaaaacaa ctttcgctac gttgctggct cgttttaaca cggagtaagt gatgtaccgt
     6421 tatttgtcta ttgctgcggt ggtactgagc gcagcatttt ccggcccgac gttggccgaa
     6481 ggtatcaata gtttttctca ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc
     6541 ggtacgtttt attgcggatg taaaattaac tggcagggca aaaaaggcgt tgttgatctg
     6601 caatcgtgcg gctatcaggt gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa
     6661 cacgtcgttc ccgcctggca attcggtcac cagcgccagt gctggcagga cggtggacgt
     6721 aaaaactgcg ctaaagatcc ggtctatcgc aagatggaaa gcgatatgca taacctgcag
     6781 ccgtcagtcg gcgaggtgaa tggcgatcgc ggcaatttta tgtacagcca gtggaatggc
     6841 ggcgaaggcc agtacggtca atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa
     6901 ccaccagcgc gtgcacgcgg tgccattgcg cgcacctact tctatatgcg cgaccaatac
     6961 aacctgacac tctctcgcca gcaaacgcag ctgttcaacg catggaacaa gatgtatccg
     7021 gttaccgact gggagtgcga gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac
     7081 ccgtatgtgc aacgcgcttg ccaggcgcga aagagctaac ctacactagc gggattcttt
     7141 ttgttaaccc ctaccccacg cgtacaaccg cgtggggaga cgacgcggat ttttaactat
     7201 gcgtatcccc cgcatttatc atcctgaacc actgaccagc cattctcaca tcgcgctttg
     7261 cgaagatgcc gccaaccata tcgggcgcgt actgcgcatg gggccggggc aggcgttgca
     7321 attgtttgac ggtagcaacc aggtctttga cgccgaaatt accagcgcca gcaaaaaaag
     7381 cgtggaagtg aaggtgctgg aaggccagat cgacgatcgc gaatctccgc tgcatattca
     7441 cctcggtcag gtgatgtcgc gtggtgaaaa aatggaattt actatccaga aatcgatcga
     7501 actcggtgta agcctcatta cgccactttt ttctgagcgc tgcggcgtta aactggatag
     7561 tgaacgtctg aacaagaagc ttcagcagtg gcagaagatt gcaattgctg cctgtgagca
     7621 gtgtggtcgt aaccgggtgc cggaaatccg tccagcgatg gatctggaag cctggtgtgc
     7681 agagcaggat gaaggactga aactgaatct tcacccgcgc gccagtaaca gcatcaatac
     7741 gttgccttta ccggttgaac gcgtccgcct gctgattggc ccggaaggcg gtttatcggc
     7801 agatgaaatt gccatgactg cccgctatca atttactgat atcctgttgg gacctcgcgt
     7861 tttgcgtaca gagacaactg cgctcaccgc cattaccgcg ctacaagtac gatttggcga
     7921 tttgggctaa cggagaagaa taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa
     7981 catcaacatc aagaaagatt ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta
     8041 cgaacttcac tatatggaga tgggcgatct gtatctgatc aatggcgaag cccgcgccca
     8101 tacccgcacg ctgaacgtga agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca
     8161 ggatctgccg ctggccgatc tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac
     8221 cgagtttatc tacgcgacct atattctgga acgtgccgaa gagaaaggga cgctgatcgt
     8281 taacaagccg cagagcctgc gcgactgtaa cgagaaactg tttaccgcct ggttctctga
     8341 cttaacgcca gaaacgctgg ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa
     8401 acacagcgac atcattctta agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt
     8461 gaaagaaggc gatccaaacc tcggcgtgat tgccgaaacc ctgactgagc atggcactcg
     8521 ctactgcatg gcgcaaaatt acctgccagc cattaaagat ggcgacaaac gcgtgctggt
     8581 ggtggatggc gagccggtac cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg
     8641 tggcaatctg gctgccggtg gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa
     8701 aatcgcccgt cagatcgggc cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga
     8761 tatcatcggc gaccgtctga ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat
     8821 tgaagcagag tttccggtgt cgatcaccgg aatgttaatg gatgccatcg aagcacgttt
     8881 acagcagcag taacccacct tagcgagaag gatctcgttg agactctgag tgacagcgcc
     8941 cttctttcca cgcatactgg gcgctgttgc ttttttgaac caggaaacag aacctctgac
     9001 aatgaattta cagcatcact ttcttattgc catgcctgct ctccaggatc cgattttccg
     9061 tcgttccgtg gtctacattt gcgaacataa taccaatggt gcaatgggga tcatcgtcaa
     9121 caagccgctg gaaaatctca aaattgaagg gattctggaa aagctgaaga tcacgccgga
     9181 gccgcgtgat gaatcaatcc gtctggataa accggttatg ctcggcggcc cactggctga
     9241 agatcgcggg tttattttgc atactccgcc ctccaatttt gcttccagca ttcgcatttc
     9301 agacaacacg gtgatgacca cttcccgcga tgtgctggaa acgctcggca ccgataaaca
     9361 accgtctgac gtattggtgg ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca
     9421 agaaattctc gataacgcgt ggctaacggc cccggcggat ctgaatattc tgttcaaaac
     9481 gccgattgcc gaccgctggc gcgaggcggc aaaactgatt ggtgtggata ttctcaccat
     9541 gcctggtgtg gcaggacacg cctgatgagt ggaaccttac tcgccttcga cttcggcacc
     9601 aaaagcattg gcgtagcggt cggccaacgc attaccggca ccgctcgccc tttgcctgca
     9661 attaaagcgc aggacggtac gccggactgg aacctcatcg aacgtttact gaaagagtgg
     9721 cagcctgacg aaatcatcgt cggtttgccg ctgaatatgg acggcaccga acagccgtta
     9781 accgccagag cgcgcaaatt tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag
     9841 ctccatgacg agcgtcttag cactgtggaa gcccgttccg gtctttttga acagggcggc
     9901 tatcgggcgc tcaacaaagg caaagttgac tctgcctctg cggttattat tctcgaaagc
     9961 tatttcgagc agggatatta aggcgatcta aaagcctggc ggatagtcgt taccgccatc
    10021 ttgccggatg cggcgtgaac gccttatcca gcacacatct ggcagcggct gtaggtctga
    10081 taagacgcgt tagcgtcgca tcagacattt attgccttta taggccctat aaacttgcgc
    10141 atcgggcatg gcaacgtcac aaacgccctt cccccacccg ctgctgataa ctctgctgaa
    10201 acgttaacat ccccacctgc tgcccggttt gaataacatg cggtaactgg tgggttttcc
    10261 cttcgcgaat caaattcccc accgcgggag tgttaatcag taattcaaac agcgccacgc
    10321 gtccttcctg tttatccact tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa
    10381 gttgattacg tacgggatct ttttcctgcg ccggaaatga atccaccagt cgctcaactg
    10441 cctgcgcggc accacgtgta tgtaaggttg ccagcaccag atgcccggtt tctgccgccg
    10501 tcagcgccag acggattgtc tcactgtcac gtagctctcc gagcaaaatc acatcaggat
    10561 cttcccgcaa tgcagcccgc aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc
    10621 gctgctggat caaacatcgc tggctggcat agagatattc cacaggatct tccagcgtca
    10681 gaatatgcgc atcggcatgt tgattaagat agccaaccat cgccgccagc gtggtagatt
    10741 tgccactccc cgtagccccc gtcaccagaa tcaggccatt ctcgctcttg agtaattccg
    10801 gcaataccgg tggcgcacca agctgttcga gctgtggaca gtgcgaaggt aacaaccgta
    10861 atgccagaga aataccttgc cgttgcgcga acgcactgcc acgcaaccgc tggttttccg
    10921 ccagcgacac ggcaaaatcc agctggccat tttccagcaa tattgcccgc tgatcgtcat
    10981 ccagccactc ccgcagtagc tcttcgacgt ccggcgcatc aaacggcgca gcttccattc
    11041 tgccacgaat gcgccatcgt gcgggccagg cgctgcacag gtgtagatcc gagacgttat
    11101 gctttacact aagggccaca atttcttcca tattcatact aagatcctcg gaaaatgaac
    11161 gatattgcgc ataacctggc acaggtccgg gacaaaatct cagcggctgc aacgcgttgc
    11221 ggccgttctc cagaagaaat tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc
    11281 atcgcagaag ccattgatgc cgggcagcgt caatttggtg aaaactacgt tcaggaaggg
    11341 gtagataaaa ttcgccactt tcaggaactg ggcgtaacag gattagaatg gcattttatt
    11401 ggcccgttgc agtctaataa aagccgccta gtggcagagc atttcgactg gtgtcatacc
    11461 atcgaccgtt tgcgcattgc tacccgcctc aacgatcagc gcccggcaga acttccccct
    11521 cttaacgtac tgattcaaat taatattagt gatgaaaaca gtaagtccgg gattcaactg
    11581 gcagaactgg acgagctggc agcggcggtc gctgaactac cgcgtttacg tctgcgcggg
    11641 ctgatggcaa tccctgcgcc tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa
    11701 atggctgtag catttgccgg actgaaaacg cgctacccgc atatcgacac gctctctctg
    11761 ggaatgtcgg acgatatgga agccgccatt gcggcaggta gcacgatggt tcgtatcggc
    11821 actgcaattt ttggtgcgcg tgattactct aaaaaataag gaattaaagg aacgccatga
    11881 atacgttgac tttcctgctt tcaacggtca ttgagctgta taccatggtg ctgttattac
    11941 gcatctggat gcagtgggct cattgtgatt tttacaaccc cttctcacag tttgtagtga
    12001 aggtagcgca gccaattatc gggccactgc gccgcgttat tccggcaatg ggtcccattg
    12061 acagcgcctc gctgctggtt gcctatattc tcagttttat caaagccatt gtgctgtttg
    12121 aagtggtgac cttcctgcca atcatctgga ttgccggttt actgattctg ctgaaaacca
    12181 tcggcttgct gattttctgg gtcctgctgg tgatggcgat tatgagctgg gtaagccagg
    12241 gtcgtagccc gattgaatac gtgctgattc agctggccga tccgctgctg cgcccgattc
    12301 gccgcttgct accggcaatg ggtgggattg atttctcgcc gatgatcctc gttctgctgc
    12361 tgtatgtcat caatatgggt gtcgcagaag tattacaggc aaccggaaac atgctgctgc
    12421 cggggctgtg gatggcgtta tgagtgccgt aacagttaat gatgacggtc tggttttacg
    12481 gctctatatt cagccgaaag ccagccgtga ttctattgtc ggtttacatg gcgacgaagt
    12541 taaagtcgcc attaccgcgc cgccggttga cggccaggcc aacaatcatc tggtgaagtt
    12601 tctcggtaag caattccggg ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg
    12661 ccgccacaaa caaattaaaa tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt
    12721 aattaattag gtatcctatg caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc
    12781 gtgagctggc gtcgctgctt agcgactttg gtcttgatat cgtggcccaa acagacctcg
    12841 gcgttgattc cgctgaagaa accggcctaa cctttatcga aaacgcgatt ctgaaagcgc
    12901 gccatgcggc aaaagtgacc ggtttaccgg caattgccga cgactctggt ctggcggtag
    12961 atgcgctggg tggcgcgccg ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg
    13021 atcaaaagaa tctgcaaaaa ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc
    13081 aggcgcgttt ccactgcgtg ctggtatatc tgcgtcacgc ggaagatccg actccgctgg
    13141 tatgccacgg cagctggccg ggcgtgatta ctcacgaacc agcgggcact ggtggctttg
    13201 gttatgatcc aatcttcttc gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg
    13261 aagaaaagag cgccatttcc caccgtggtc aggcgttgaa actgctgctg gacgctttac
    13321 gtaatggtta aattaccgcc gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa
    13381 tgcccgtact gcgatttcaa ctctcacgcg ttgaaaggag aagtgccgca cgacgattat
    13441 gttcagcatc tgctttgcga tctggacaat gatgtggctt acgctcaggg ccgtgaagta
    13501 aagacaatct ttattggcgg tggtacgccg agcctgcttt ccggcccggc gatgcaaacg
    13561 ctgctagacg gcgtgcgttc gcgtttgccg ctggcagcgg atgcagaaat tactatggaa
    13621 gcgaacccag gcacggtaga agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac
    13681 cgcatctcta ttggtgtgca gagttttagc gaagaaaagc tgaaacgact tgggcgtatt
    13741 catggcccgc aagaagcgaa acgagcggca aagctggcga gcggtttagg gttacgtagc
    13801 tttaaccttg atttgatgca tggactaccg gatcaatcac tggaagaggc gcttggcgat
    13861 ctacgccagg ccattgaact gaatccgccg catctttcct ggtatcaact gaccatcgaa
    13921 cctaatacgc tgtttggctc gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat
    13981 attttcgaac aggggcatca gttattaacc gcagcgggtt atcagcaata tgaaacttcc
    14041 gcttacgcca aacccggtta tcagtgccag cacaatctca actactggcg ctttggtgac
    14101 tacatcggta ttggctgcgg cgcgcacggc aaagtgacct tcccggatgg gcgcattctg
    14161 cgtaccacta aaacgcgtca tcctcgtggt tttatgcaag gaaggtatct ggaaagccag
    14221 cgtgatgtcg aagccgcaga taagccgttt gagttcttta tgaatcgctt ccgtctgctg
    14281 gaggccgcgc cgcgcgtgga gtttattgcg tataccgggc tttgcgaaga tgtgattcgc
    14341 ccacagttag acgaggcgat tgcccagggt tatctcaccg aatgtgcgga ttactggcag
    14401 ataacggaac atggtaaatt attcttaaac tcacttcttg aattattcct cgctgagtaa
    14461 tcattaatgc gcctgcgggc gcattaatca cttcttttat ccccccgtta attaacctcg
    14521 gcagaaatat atttcttact gaataaatat cgacgacatt tattatatat atttagtgaa
    14581 tatttactaa tcattcattg attataaata tgtacaacct ggcgcacaaa tttaatattt
    14641 aatttctgac tttatttcaa gttgtgataa acatagacaa gttgtcagac aggttaacca
    14701 atttgtcgca caaagcactg ataagcacaa ttagatatca accaatttcc atccttcaac
    14761 agggagtttt tttatgaaaa tgaggtcatt tacccgctca ttagtctgcg catccttgct
    14821 ggctctggtt agcactggcg taaacgccgc agaaaaagtg acgttaaagt tagcacacaa
    14881 cctggaaaga agtcatgttg tacatcagag ttttgaagag ctggcgaaag aggtaaaaca
    14941 gctgtcaaaa ggtaatatgg tgatccgtat ttatcccagc agccagatgg gtaatgcgcg
    15001 agaaactatg gaattgttgc aaaatggcgc actggatatg accaaaggtt ccgccagtga
    15061 tctggagtct tttgataata tttatgctat ctataatttg ccgttcttat taagttgttt
    15121 ttggtgaagt tggtaaagaa attatggact caacaaaaga caaaggcttt tttgctttat
    15181 cagcttatgt agcgggtact cgcagtttct atgcgaaaaa accgattaca aagccagaag
    15241 atcttaaagg actgaaaatt cgtgttcagc caagtccgac caccatcaaa atgattgaac
    15301 tgatgggtgg ttcacccact ccaatttcat ttggcgaagt ttatactgcg atgcagcaag
    15361 gtgtagtaga tggcgcagaa aataacgtgc cttcctggat gcagactcga catattgaaa
    15421 ttgccaaagt tttttctgaa gatgaacacg cctcaattcc tgactttttg gttatctcca
    15481 ccaaaacatg gaataaatta cccccagaac agcaacaaat cctcgaaaca gcggcgaaga
    15541 aatccgaagc ttatcaacaa aaattgtggg aaaaaattga cgctgacaca cgtgctcagg
    15601 cgaaagctat ggggggtgaa attgtaaaag tggataaagc gccattccgt aaagccgtgc
    15661 aacctctgtt tgacgacttc aaaaaagatc caaaacaagc tgctttgtta gagaaattcg
    15721 acaacgctgc tcaataatat tccgggccag cctctggccc tgattttcca ggatgcttac
    15781 aatgatattt aaccgcctga aacttgcggt agaccgcgtc attgccgcgt tttctgtcgc
    15841 cgtaatgctt gctctggttg tatgcgtagt ctggcaagta tttagccgct acgtacttaa
    15901 ccagccaagt acattaaccg atgagctggc acgctttttg atgatctggg taggattatt
    15961 aggtgctgca tataccgtcg gcgcgcagcg acatctctct attgatttat ttgctctcgc
    16021 cctgaataag cgcaaacaat tacttcttag tatagtcatt aacgttctca ttttaggttt
    16081 tgccggttcg gtcattgtga ctggtggcct gaaattaatt gataaaacac tggcaacatc
    16141 acaggtatct gccgctatgc aaataccgat gggatacgta tatatcattc tgccattaag
    16201 tggagtggta atgatgtttt atgc
//
LOCUS       DAJVQH010000087        16152 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJVQH010000087 DAJVQH010000000
VERSION     DAJVQH010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.087"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(188..520)
                     /gene="emrE"
                     /locus_tag="OQM87_002821"
     CDS             complement(188..520)
                     /gene="emrE"
                     /locus_tag="OQM87_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCS1508891.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(865..1179)
                     /gene="fliE"
                     /locus_tag="OQM87_002822"
     CDS             complement(865..1179)
                     /gene="fliE"
                     /locus_tag="OQM87_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCS1508892.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1393..3051
                     /gene="fliF"
                     /locus_tag="OQM87_002823"
                     /pseudo
     CDS             1393..3051
                     /gene="fliF"
                     /locus_tag="OQM87_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            3044..4039
                     /gene="fliG"
                     /locus_tag="OQM87_002824"
     CDS             3044..4039
                     /gene="fliG"
                     /locus_tag="OQM87_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCS1508893.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4032..4718
                     /gene="fliH"
                     /locus_tag="OQM87_002825"
     CDS             4032..4718
                     /gene="fliH"
                     /locus_tag="OQM87_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCS1508894.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            4718..6091
                     /gene="fliI"
                     /locus_tag="OQM87_002826"
     CDS             4718..6091
                     /gene="fliI"
                     /locus_tag="OQM87_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCS1508895.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRSQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6110..6553
                     /gene="fliJ"
                     /locus_tag="OQM87_002827"
     CDS             6110..6553
                     /gene="fliJ"
                     /locus_tag="OQM87_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCS1508896.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6550..7677
                     /gene="fliK"
                     /locus_tag="OQM87_002828"
     CDS             6550..7677
                     /gene="fliK"
                     /locus_tag="OQM87_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCS1508897.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSASLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGDDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7782..8246
                     /gene="fliL"
                     /locus_tag="OQM87_002829"
     CDS             7782..8246
                     /gene="fliL"
                     /locus_tag="OQM87_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCS1508898.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8251..9255
                     /gene="fliM"
                     /locus_tag="OQM87_002830"
     CDS             8251..9255
                     /gene="fliM"
                     /locus_tag="OQM87_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCS1508899.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDILPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9252..9665
                     /gene="fliN"
                     /locus_tag="OQM87_002831"
     CDS             9252..9665
                     /gene="fliN"
                     /locus_tag="OQM87_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCS1508900.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9668..10033
                     /gene="fliO"
                     /locus_tag="OQM87_002832"
     CDS             9668..10033
                     /gene="fliO"
                     /locus_tag="OQM87_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCS1508901.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10033..10770
                     /gene="fliP"
                     /locus_tag="OQM87_002833"
     CDS             10033..10770
                     /gene="fliP"
                     /locus_tag="OQM87_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCS1508902.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10780..11049
                     /gene="fliQ"
                     /locus_tag="OQM87_002834"
     CDS             10780..11049
                     /gene="fliQ"
                     /locus_tag="OQM87_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCS1508903.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11058..11762
                     /locus_tag="OQM87_002835"
     CDS             11058..11762
                     /locus_tag="OQM87_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HCS1508904.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFNGHLWLISLLVDTFHTLPIGSEPLNSNAFLAPTKAGSLIFLNGLMLA
                     LPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFS
                     EIFNLLADIISELPLI"
     gene            12047..12670
                     /gene="rcsA"
                     /locus_tag="OQM87_002836"
     CDS             12047..12670
                     /gene="rcsA"
                     /locus_tag="OQM87_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCS1508905.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12714..12902)
                     /gene="dsrB"
                     /locus_tag="OQM87_002837"
     CDS             complement(12714..12902)
                     /gene="dsrB"
                     /locus_tag="OQM87_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCS1508906.1"
                     /translation="MKVNDRVTVKTDSGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13065..13292
                     /gene="yodD"
                     /locus_tag="OQM87_002838"
     CDS             13065..13292
                     /gene="yodD"
                     /locus_tag="OQM87_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCS1508907.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13590..14405
                     /locus_tag="OQM87_002839"
                     /pseudo
     CDS             13590..14405
                     /locus_tag="OQM87_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(14402..16096)
                     /gene="yedQ"
                     /locus_tag="OQM87_002840"
     CDS             complement(14402..16096)
                     /gene="yedQ"
                     /locus_tag="OQM87_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCS1508908.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDHA"
BASE COUNT         4023 a         4156 c         4237 g         3736 t
ORIGIN      
        1 aaacgataag ttttttcata aaaacctcat tgttgtgacc tatcgttatt ttatttgatg
       61 tgccttgatc tcattatgca taaaggcaca atgatcgcgc aaatgcaatc ataattataa
      121 aggtgccccg aacattagcc ataatatgca caaatcagat tcctgttatc tcaaggagaa
      181 ttatttttta atgcggtgca cttcgtgaca acaagttaat gactaacaca ccggcacaaa
      241 tcaacatcat gcctatgata gctggcaggt ccagtcgttg accaaaaaat ccccaggaaa
      301 gcaaactaat cagaacgata ccgactcctg accagatagc ataagcaatc cctgtaggta
      361 tataagccag cgtctgagct aataaccaga atgatgcaca atagcaaata attgtgccaa
      421 cagagggcca taaccgtgta aacccctctg aaaacttcat taaggttgta ccaatgacct
      481 ctgcaagtat tgcgccacca agataaatat aagggttcat agcatattct ttcctgttca
      541 tactggagag aattgtacta cagtttgaac acaactcacc gttttcatcc tagtgtatgt
      601 gtatccaagg ggctctggcg tggcaagaga taaaggcagg aacaataatg atcagaagga
      661 accccaggct aacgaatgag ttcactcttc ctgggagcaa agcgaagaca aaacatgtta
      721 tcgcgtttac ggatcgtgga tggcagaaaa caaccaggat caggtatcca ttttaaacca
      781 gaaattgagt gagtttgccc catccatgcc ccacgctgtg ggatcggata taattaaaca
      841 agcatagtca acaggttaca caacctacac ctgcatagac atcacttcct gatacgccgc
      901 caccagctta ttacgcacct gaatccccat ttgcatagaa actgaggctt tttgcatatc
      961 ggtcatcaca tcgtttagcg ccacgccggg ttcaccgagg gtgaattttt ctgcctgcgt
     1021 gcgggcgact gtttgtgtat cgcttatgcg atcgatcgcg gcgtgcagct gcccggcaaa
     1081 actaatggtc ggttgcggca gtgattcctg cgcacgcgca ctcatcgccg tagcctgtaa
     1141 ctggctgata accccttcaa tcccctgtat cgctgacatt ttcgtctccc ggataatttc
     1201 tggtaacaaa gcctaccagt aagtcaataa gacaaaggcg ctaaatagca acaaaaaaac
     1261 gggtttattg gcggatagaa aaaacgaaag cacaaataat gggagcgtca atttttcgag
     1321 tttgctgacc cgagagtgag tcttgttcca ctttgccaat aacgccgtcc ataatcagcc
     1381 acgaggtgcg cgatgaatgc gactgcagcc cagacaaaat ctcttgagtg gcttaatcgc
     1441 ctgcgtgcga atccgaaaat tccattgatt gttaccggtt ccgcggcagt ggcggtcatg
     1501 gtcgcactga tcctgtgggc gaaagccccc gactaccgca cattattcag caatctttcc
     1561 gatcaggatg gtggcgcaat tgtcagccaa ctgacgcaaa tgaatattcc ttaccgcttc
     1621 agcgaagcca gcggcgctat tgaggttccg gcagataaag ttcacgaact gcgcctgcgc
     1681 ctggcgcaac aaggtttgcc caaaggcggc gcggtcggtt tcgaactgct cgatcaggaa
     1741 aagtttggta tcagccagtt cagcgaacag gtgaattatc agcgggcgct ggaaggcgag
     1801 ctttctcgta ccatcgaaac tatcggcccg gtaaaagggg cgcgcgtaca tctggcaatg
     1861 ccgaaaccgt ctttattcgt ccgtgaacaa aaatcccctt ctgcatcggt gacggtaaat
     1921 ctgttaccca gccgcgcact cgatgaaggg caaattagcg ctattgtaca tctggtttcc
     1981 agcgcggttg ctggtctgcc gcctggaaac gtcacgctgg tggatcaggg cgggcatctg
     2041 ttaacccagt ccaataccag cgggcgcgat cttaatgacg ctcagttgaa atatgccagc
     2101 gatgtcgaag gccgtattca gcggcgtatt gaagcgatcc tgtcgcctat tgttggtaac
     2161 ggtaatattc acgcctaggt cacggcgcaa ctggacttcg ccagtaaaga acaaacggaa
     2221 gaacagtatc gccctaacgg tgatgaatct catgcggcgc ttcgttcacg ccagcttaat
     2281 gagagcgagc aaagcggttc cggttatccg ggcggcgtac cgggggcgtt gtcgaatcaa
     2341 ccggcacctg cgaataacgc gccaatcagc acgcctccga caaatcaaaa taaccgccag
     2401 cagcaggcga gcaccaccag caatagtggg ccgcgtagca cacagcggaa tgaaaccagt
     2461 aactacgaag tcgatcgcac cattcgtcat accaaaatga acgtgggcga tgtgcaacgt
     2521 ctgtcagtcg cggtagtggt gaattacaaa accttgccag acggtaaacc attgcctctc
     2581 agcaacgaac agatgaagca aattgaagct ctgacccgcg aggcgatggg cttttctgaa
     2641 aaacgcggtg attcgctcaa tgttgttaac tcgccgttca atagcagtga cgaaagcggc
     2701 ggagcactgc cgttttggca acagcaagtg tttatcgatc agttgctggc tgccggtcgc
     2761 tggttgctgg tactgctggt ggcgtggttg ctgtggcgga aagcggtacg cccgcagcta
     2821 acacgtcgcg ccgaggcggt gaagactgtg cagcaacagg cgcaggcccg cgaggaagtg
     2881 gaagatgcgg tggaagtccg tctgagcaaa gacgaacaac tccaacaacg gcgcgctaac
     2941 caacgactgg gggcagaagt catgagccag cgtatccgtg aaatgtctga taacgatccg
     3001 cgcgtggtgg cgctggtcat tcgccagtgg ataaataacg atcatgagta acctgacagg
     3061 caccgataaa agcgtcatcc tgctgatgac cattggcgaa gaccgggcgg cagaggtgtt
     3121 caagcacctc tcccagcgcg aagtgcaaac cctgagcgct gcaatggcga acgtcacgca
     3181 gatctccaac aagcagctaa ccgatgtgct ggcggagttt gagcaagaag ctgaacagtt
     3241 tgctgcactg aatatcaacg ccaacgatta tctgcgttcg gtattggtca aagctctggg
     3301 tgaagaacgt gccgccagcc tgctggaaga tattctcgaa acccgcgata ccgccagcgg
     3361 tattgaaacg ctcaacttta tggagccgca gagcgccgcc gatctgattc gcgatgagca
     3421 tccgcaaatt atcgccacca ttctggtgca tctgaagcgc gcccaggccg ccgatattct
     3481 ggcgttgttc gatgaacgtc tgcgccacga cgtgatgttg cgtatcgcca cctttggcgg
     3541 cgtgcagcca gccgcgctgg ccgaactgac cgaagtactg aatggcttgc tcgacggtca
     3601 gaatctcaag cgcagcaaaa tgggcggcgt gagaacggca gccgaaatta tcaacctgat
     3661 gaaaacgcag caggaagaag ccgttattac cgccgtgcgt gaattcgacg gcgagctggc
     3721 gcagaaaatc atcgacgaga tgttcctgtt cgagaatctg gtggatgtcg atgatcgcag
     3781 cattcagcgt ctgttgcagg aagtggattc cgaatcgctg ctgatcgcgc tgaaaggggc
     3841 cgagcagcca ctgcgcgaga aattcctgcg caatatgtcg cagcgtgccg ccgatattct
     3901 gcgcgacgat ctcgccaacc gtggtccggt gcgtctgtcg caggtcgaaa acgaacagaa
     3961 agcgattctg ctgattgtgc gccgtcttgc cgaaactggc gagatggtga ttggcagcgg
     4021 cgaggatacc tatgtctgat aatctgccgt ggaaaacctg gacgccggac gatctcgcgc
     4081 caccaccggc agagtttgtg ccaatggtcg agtcggaaga aaccattatt gaagaggccg
     4141 aacccagcct tgagcagcaa ctggcgcaac tgcaaatgca ggcccacgag cagggttatc
     4201 aggcggggat tgccgaaggt cgccagcaag gtcacgaaca gggctatcag gaaggactgg
     4261 cccaggggct ggaacaaggt ctggcagagg cgaaggctca acaagcgcca attcatgccc
     4321 ggatgcagca actggtcagc gaatttcaaa ctacccttga tgcgcttgat agtgtgatag
     4381 cttcgcgcct gatgcagatg gcgctggagg cagcacgtca ggtgatcggt cagacaccaa
     4441 cggtggataa ctcggcactg atcaaacaga tccaacagtt gttgcagcaa gaaccgttat
     4501 tcagcgggaa accacagctg cgcgtacacc cggatgatct gcaacgtgtg gatgatatgc
     4561 tcggcgcaac tttaagtttg catggctggc gcttgcgggg cgatcccacc ctccaccctg
     4621 gcggatgtaa agtctccgcc gatgaaggcg atctcgacgc cagtgtcgcc actcgctggc
     4681 aagaactctg ccgtctggca gcactaggag tggtgtaatg accacgcgcc tgactcgatg
     4741 gctaaccacg ctggataact ttgaagcaaa aatggcgcag ttgcctgcgg tacgtcgcta
     4801 cgggcgatta acccgcgcta ccgggctggt gctggaagcc accggattac aattgccgct
     4861 cggcgcaacc tgtgtcattg agcgccagaa cggcagcgaa acgcacgaag tagaaagcga
     4921 agtcgttggc tttaacggtc aacggctgtt tttaatgccg ctggaggaag tggaaggtgt
     4981 cctgcccggc gcgcgtgtat atgccaaaaa catttcggca gaagggctgc aaagcggcaa
     5041 gcagttgccg ctcggtccgg cgttattagg tcgtgttctg gacggtagcg gtaaaccgct
     5101 cgatggcctg cccttccccg atacgacgga aaccggtgcg ctgattaccc cgccatttaa
     5161 cccgttgcaa cgtacaccga ttgaacatgt gctggacacc ggcgtgcgcc caatcaatgc
     5221 cctgcttacc gtcggacgcg ggcagcgtat ggggctgttt gccgggtcgg gcgttggtaa
     5281 aagtgtgctg ctggggatga tggcacgtta cacccgcgcc gatgtcattg tcgtgggttt
     5341 gattggtgaa cgtgggcgcg aagtaaaaga ttttattgag aatatcctcg gtgccgaagg
     5401 acgtgcacgc tcagtggtga ttgccgctcc ggcggatgtt tctccgctcc tgcgaatgca
     5461 aggtgccgcc tatgccacgc gcattgccga agattttcgc gatcgtagtc agcatgtgtt
     5521 gctgattatg gactccctca cccgctacgc gatggcccag cgtgaaattg cgctggcgat
     5581 tggcgaacca cctgccacta aaggttatcc accgtcggtg tttgccaaat taccggcact
     5641 ggtcgagcgt gccggtaatg gcattagcgg cggcggctcg attaccgcgt tttataccgt
     5701 gctcaccgaa ggcgatgacc agcaggaccc cattgctgac tccgcgcggg caatcctcga
     5761 cggtcacatt gtgctgtctc gccgactggc ggaagccggg cactatccgg ctatcgatat
     5821 tgaagcgtcg atcagtcgcg caatgacggc gttgatcagt gagcaacatt acgcgcgagt
     5881 gcgcaccttc aaacagctgt tgtcgagttt tcagcgtaac cgcgatctgg ttagcgtcgg
     5941 cgcgtatgcc aaaggcagcg atccgatgct cgataaagcc atcgccctgt ggccgcagct
     6001 ggagggctat ttgcaacaag gcatttttga acgcgcggac tgggaagcgt ctctccaggg
     6061 gctggagcgt attttcccga cagtgtcata acccaggaga taacggcaga tggcggaaca
     6121 tggtgcgctg gcgaccctga aagatctggc agaaaaagag gtagaggatg ccgctcgcct
     6181 gctgggtgaa atgcgtcgcg gatgtcagca ggcggaagaa cagctcaaaa tgctgatcga
     6241 ttatcagaat gaatatcgca ataacctcaa tagcgatatg agtgccggga tgaccagcaa
     6301 ccgctggatc aactatcagc agtttatcca gacgctggaa aaagccatta ctcagcatcg
     6361 ccagcaactt aatcagtgga cgcagaaagt tgacattgcc ctgaacagct ggcgagaaaa
     6421 gaaacaacgt ttgcaggcct ggcagacact gcaggaacgg caatccacgg cggcactgct
     6481 tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg ccgccatgag
     6541 gaaacctgaa tgattcgctt agcgcccttg attaccgccg acgttgacac caccacattg
     6601 cctggcggca aagccagcga tgctgcacaa gattttctaa cgttgttgag cgaagcatta
     6661 gcaggcgaga caacaactga caaagcggcc ccccagttgc tggtggcaac agataagccc
     6721 acgacaaaag gcgagccgct ggtcagcgat attcttgccg atgcgcaaca agcggattta
     6781 ctgatccctg tggatgaaac acttcctgtc atcaacgacg aacaatccac atcaacaccg
     6841 ttaaccaccg ctcagacaat gacgttggct gcggtggctg acaaaaatac gacaaaagac
     6901 gaaaaagcgg atgatcttaa tgaagacgtc accgcaagtc tgagcgccct ttttgcgatg
     6961 ttgccgggtt ttgacaatac gcccaaagtg actgatgcac cgtcaaccgt gttaccggca
     7021 gagaaaccaa cgctcttcac aaaactgact tctgcgcaac tcactacagc acagcctgat
     7081 gacgcccccg gcacaccagc tcagccatta acaccgctgg tagcagaagc ccagagtaaa
     7141 gcggaagtca tcagcacgcc ttcgccggtg accgctgacg ccagcccgct aatcactcca
     7201 caccagacac agccactgcc caccgtcgcc gcgcctgttt tgagtgcatc gctgggttct
     7261 cacgaatggc aacaatcatt gagccagcat atttcgctgt tcacccgcca ggggcaacaa
     7321 agtgcagagt tgcgtctgca cccacaggat ttaggtgaag tgcaaatttc cctcaaagtg
     7381 gatgataacc aggcgcagat ccagatgatt tcaccgcatc agcacgtacg cgccgccctg
     7441 gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa gtggcattca gttagggcaa
     7501 agcaacatta gtggcgaaag ctttagtggt cagcagcagg ccgcttccca gcaacagcaa
     7561 agccaacgca cagcaaacca tgaacctctg gcgggggatg acgacgatac gcttccggtt
     7621 cccgtctctt tacaagggcg tgtaacaggt aacagcggcg ttgatatttt cgcctaacgt
     7681 cagaggtagc accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag acgcaggata
     7741 attagccgat aagcagtagc gacacaggaa gaccgcaaca catgactgat tacgcgataa
     7801 gcaagaaaag caagcgatcg ctttggatcc cgattctggt attcattacc ctcgcggcct
     7861 gtgccagcgc aggttacagc tactggcatt cgcatcaggt tgccgctgac gacaaagcgc
     7921 agcaacgcgt cgtgccctcc ccggtcttct atgcgctgga tacctttacg gtcaatttgg
     7981 gcgatgcgga tcgcgtgctt tatatcggca taaccctgcg actgaaagat gaagcaaccc
     8041 gctcgcggct gagtgagtat ttgccggaag tccgtagtcg cttgctgtta ctgttttcac
     8101 gtcaggatgc tgccgtactg gcgacagaag aaggcaagaa aaacctgatt gccgagatta
     8161 aaaccacact ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc accgacgtgc
     8221 tgtataccgc ttttattctg cgataacgac atgggcgata gtattctttc tcaagctgaa
     8281 attgatgcgc tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc cagtgttagc
     8341 ggcgaaagtg acattcgtcc gtacgatccg aatacccaac gacgggttgt gcgcgaacgt
     8401 ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc attttcgtat ggggctgttc
     8461 aacctgctgc gccgtagccc ggacattacc gtcggggcca tccgcattca gccataccat
     8521 gaatttgccc gcaacctgcc ggtgccgacc aacctgaacc taatccacct gaaaccgcta
     8581 cgcggcacag ggctggtagt gttctcgccg agtctggtgt ttatcgccgt ggataacctg
     8641 tttggcggcg atggacgctt cccgaccaaa gtggaaggtc gcgagtttac ccatactgaa
     8701 cagcgcgtca tcaaccgtat gttgaagctg gcgctggaag gctatagcga cgcctggaaa
     8761 gcgattaatc cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgga atttaccaat
     8821 atcaccacct cgccgaacga cattgtggtt aacacgccgt tccatgtgga gattggcaac
     8881 ctgaccggcg aatttaatat ctgcctgcca ttcagcatga tcgagccgct acgggaattg
     8941 ttggttaacc cgccgctgga aaactcgcgt aatgaagatc agaactggcg cgataacctg
     9001 gtgcgccagg tgcagcattc acagctggaa ctggtggcca actttgccga tatctcgcta
     9061 cgcctgtcgc agattttaaa actgaaaccc ggcgacatcc tgccgataga aaaacccgat
     9121 cgcatcatcg cccatgttga cggcgtcccg gttctgacca gtcagtacgg cactctcaac
     9181 ggtcagtatg cgttacggat agaacatttg attaacccga ttttaaattc tctgaacgag
     9241 gaacagccca aatgagtgac atgaataatc cggccgatga caacaacggc gcaatggacg
     9301 atctgtgggc tgaagcgttg agcgagcaaa aatcaaccag cgaaaaaagc gctgccgacg
     9361 cggtgttcca gcaatttggc ggtggtgatg tcagcggaac gttgcaggat atcgacctga
     9421 ttatggatat tccggtcaag ctgaccgtcg agctgggccg tacgcggatg accatcaaag
     9481 agctgttgcg tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg ggcgaaccgc
     9541 tggatattct gaacaacggt tatttaatcg cccaaggcga agtggtggtt gttgccgata
     9601 aatatggcgt gcggatcacc gatatcatta ccccgtccga gcggatgcgc cgcctgagcc
     9661 gttagtgatg aataaccatg ctactgtgca atcttccgcg ccggtttctg ctgagccact
     9721 gctgcaggtg agcggcgcac tcatcgccat tattgccctg atcctcgctg ctgcctggct
     9781 ggtaaaacgg ctgggatttg cccctaaacg cactggcgtt aacggtctga aaattagcgc
     9841 cagtgcttcg ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag atgcacggct
     9901 ggtgctcggc gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc cttcagcacc
     9961 aacggaagag ataccgcaga ccgattttca gtcggtcatg aaaaatttgc ttaagcgtgg
    10021 cgggagatcc tgatgcgtcg tttgttttct gtcgcaccgg tccttctctg gctgattatg
    10081 cccctcgcct tcgcgcaact gccgggtatc accagccagc cgctacctgg cggtggacaa
    10141 agctggtcgc tcccggtgca gacgctggtg ttcatcacct cgttgacgtt cattccagcg
    10201 attttactga tgatgaccag tttcacccgc atcattattg tttttggttt attgcgtaac
    10261 gcgctgggaa cgccctccgc gccacctaac caggtattgc tggggctggc actgtttttg
    10321 acctttttta ttatgtcgcc agtgatcgac aaaatttatg tcgatgcgta ccagccattc
    10381 agcgaagaga aaatctcaat gcaggaggcg ctggaaaaag gggcgcagcc gctgcgtgag
    10441 tttatgctgc gtcagacccg cgaggcagat ttagggttgt ttgccagact ggcgaatacc
    10501 ggcccgttgc aggggcctga agctgtaccg atgcgcattt tgctcccggc ctacgtgacc
    10561 agcgagttga aaaccgcatt tcagataggc ttcacgattt tcatcccttt tttgattatc
    10621 gacctggtga ttgccagcgt gttgatggca ttggggatga tgatggttcc ccccgccacc
    10681 attgctctgc cctttaaact gatgctgttt gtactggtgg atggctggca attgctggtc
    10741 ggttcgctgg cgcagagctt ttacagctag agaggcaaaa tgacacctga atcggtcatg
    10801 atgatgggaa ctgaagcgat gaaagtcgcg ctggcactgg cagccccgct gttgctggta
    10861 gcgctggtca cgggccttat catcagtatt ttgcaggcag ccacgcagat taacgaaatg
    10921 acgctgtcgt ttattccgaa aatcatcgcc gtatttatcg ccatcattat tgccggaccg
    10981 tggatgctca atctgttgct ggattacgtc cgcaccttgt tcactaacct gccgtatatc
    11041 atcgggtagc ccgtacaatg atgcaggaga caagcgatca atggctttcc tggttaagcc
    11101 tgtacttctg gccgttactg cgcgtgctgg cgctcatttc taccgcgcca attctgagcg
    11161 aacgcagcgt gccgaaacgg gtaaaactgg gtctggcaat gatgatcacg ttcgccattg
    11221 ccccatcatt acctgccaac gatgttcctg ttttttcgtt ctttgctctg tggctggccg
    11281 tgcagcagat cctgatcggc attgcgcttg gttttaccat gcaatttgcc tttgccgctg
    11341 tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct gtcatttgcg acatttaacg
    11401 gtcatttatg gttgatttca ctgctggtcg atacctttca caccctgccg attggtagcg
    11461 aaccgttgaa cagcaatgcg tttctggcac ccaccaaagc agggagtttg attttcctta
    11521 acgggctgat gctggcgtta ccgctcatta ctctgttgct gaccctgaat ctggcattag
    11581 gtttactgaa tcgtatggcc ccgcaattat ccatttttgt tattggattt ccattaactc
    11641 tgactgtcgg catctcttta atggcggcat taatgccgtt aattgcgcct ttttgcgaac
    11701 atttattcag tgaaattttt aatttgctgg ctgatattat tagtgaatta ccattaatat
    11761 aattccgtaa cgtttatcat gctaccctaa ggattatccg aaaaataata tctgcgaaca
    11821 tcttccagga tactcctgta gcgaaatatt tgttttaagc tcactcacat atcgcaacat
    11881 ttactttaag acaattccag gcaaattata caacacttta cgggatagta agtccgcctg
    11941 aaaaattgcg agagtggcgc attaggtgac ccatgttgtt ccgtttagtc atgatgaaat
    12001 attcaggtaa ggggaattat cgttacgcat tgagtgaggg tatgccatgt caacgattat
    12061 tatggattta tgtagttaca cccgactagg tttaaccggg tatctgttga gtagaggggt
    12121 taaaaaaaga gaaatcaacg acattgaaac cgttgatgac cttgccatag cttgtgattc
    12181 acagcgtcct tcagtggtgt ttattaatga ggactgtttc atccacgatg cttctaacag
    12241 tcagcgcatc aagcacatca ttaatcaaca tcccaatacg ttatttatcg tttttatggc
    12301 aattgccaat gttcattttg atgaatatct attggtcaga aaaaatttat tgatcagttc
    12361 taaatcgatt aaaccggaat ctctcgacga tatccttggc gatattctga aaaaagagac
    12421 aacgataacc tcgtttttaa atatgccgac gttatcattg agccgaaccg aatcgagtat
    12481 gttgcgaatg tggatggcag gtcagggaac cattcaaatc tccgaccaaa tgaatatcaa
    12541 agccaagacc gtttcatcgc ataaaggtaa tattaaacgt aagatcaaaa cgcataataa
    12601 acaggttatc taccatgtcg tccgactgac ggataatgtg actaatggta tttttgtcaa
    12661 catgcgctaa cacattctga ctggtggttt cccaccagtc aggctgaata agattactct
    12721 gctttctcaa caaagatacc gtcctgatgc cctgcttcat taaagaacca gatgccgagc
    12781 gggtagtctt ccagcgcaac caggtacatt gtgccttcac taaactcctc aactgccagt
    12841 accactccag gacgacgcgg accgctatcc gttttgactg ttacccgatc attcaccttc
    12901 atcgttttcc tcctgtggct ttgtgccagt gtagaacaat ttcgtttttc tggcagcgcc
    12961 aagcgcgcgc gagtgctgat tttctcgacg gtctatactt aagagatgcc aggcggactt
    13021 aacgactggc ggcaacaaca gagtaacggt tgcgaggaaa gatgatgaaa accgcaaaag
    13081 tgtacagcga taccgcaaaa cgtgaggtca gcgtcgatgt cgatgccctg ctggcggcga
    13141 tcaatgaaat tagcgaaagc gaagttcatc gcagccagaa cgattctgaa cacgttagcg
    13201 tcgatggacg tgaatatcat acatggcgtg aattggcgga tgccttcgaa ctggatattc
    13261 atgacttcag cgtctctgaa gtgaatcgtt gaatgcacaa taaaaaaatc ccgaccctga
    13321 gggtgtcggg atgaaacttg cttaagcaag aagcacttaa aaaattcgtt acaccaggaa
    13381 atctgatgtg ttcatcacct tatctgcaat ttttttcgct gacaagaaaa tattcgcgct
    13441 atgaatgatt aacgctactt atgaatagaa atgtgacgcg cgtcacccct tggtggctct
    13501 ggtgtcaacg ggatcgctcc cgaaaaaatc atctttgatt atttgtgctg ttttgagaat
    13561 catcctggta aggggagtac tgcgcgacca tgttttcaat tcaacaacca ctactggttt
    13621 ttagcgatct tgatggcacc ctgctggaca gtcatagtta tgactggcaa ccggcagccc
    13681 cctggctcag ccgtttacac gaagccaata ttcccgtcat tctctgtagc agtaaaactt
    13741 cagcggaaat gctgtacttg caaaaaatgt tggggctaca aggcttaccg ctgattgcag
    13801 agaatggcgc agtgatccag cttgctgaac aatggcagga tatagacggt tttccacgca
    13861 tcatctcagg tattagccat ggcgaaatct gccaggtttt aaatacgcta cgcgagaaag
    13921 aacattttaa attcacgact tttgatgatg tcgacgatgc aaccattgcc gaatggacgg
    13981 gattaagccg tagctaggcg gcgctgacgc agctgcatga ggcgtcggta acgctaatct
    14041 ggcgcgacag tgacgagcgt atggcacaat ttatcgctcg tctgaacgaa ctgggcttgc
    14101 agtttatgca gggtgcgcgc ttctggcacg tactggatgc ctctgccgga aaagatcagg
    14161 ctgccaactg gattatcgcg acctatcaac aattgtcagg cagacgccca accacacttg
    14221 gcctgggcga tgggccaaac gatgcgcctt tactggaggt aatggattac gcggtgattg
    14281 tgaaagggct aaaccgtgaa ggggtacatc tgcatgatga ggatccggcc cgcgtctggc
    14341 gaacgcagcg tgaaggaccg gaaggctggc gtgaagggct ggaccatttt ttctccgccc
    14401 gttaagcgtg atcgctcgcg aatacccgat tgcgcccggc ctgttttgcg aggtaaagcc
    14461 gacggtcggc caatgactgg agttgttcaa aatcataatc accggtttcc tcgctgctac
    14521 ttacccccag cgaggcactg atgcgtatcg ttgtactctt ggcgatcagc atctcttttt
    14581 catttaactt caggcgaata cgttctgcga cctccgcagc ctgcgtcaga ttcgcgcctg
    14641 gcagaatcac acaaaactcc tcaccaccga cccgcccggc aacgtcctgc gcacgcaagg
    14701 aactgctaat taatccggca gcatgagaaa gaacacggtc gcccgcctga tgaccaaagc
    14761 ggtcattaat cgctttaaaa tggtcaagat cgacctgaat gacagaaaaa ggatgttggt
    14821 gcgtctgaca caatttagcg agcggacggg ctttttcgaa cagtgcgcca cggttatata
    14881 aacgcgttaa ggtgtcgtgc cacgcctgcc actgcaacga gctttgcaga acatacatgt
    14941 tgctgaccat ccggcgaatc acataccagg aaaggagtaa catggtggta aagagcgccc
    15001 acagcagggt taatgcaatg ctgatactgc cgaaatcgcc gcgcacgcct tcgcttagcg
    15061 tatggacgcg cgccagcaca ccgtcgaaat ggtccagacg ttcccagcta acatagcgac
    15121 tgtccatacg aatgccgccc cgcgtgtcat gttccattgc ctgcgccagc aaggccagtt
    15181 cacgaggatc aaaaatattc cctgttggat ggtcaggatt ggaagaggtc aaaaatatca
    15241 gcttactgtc atagagctga tactcaccat cgaggttttt atcgatggcg tttcttaaaa
    15301 attgctgcat agtacgcacg ggaatactca tccccagcac gccataccag tagttattac
    15361 tgtctaccgg aacactgacg gtaacctgcg gttcagtatt gctggcgtgt tccggttgcg
    15421 aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg cgaatggccg ataaaccagg
    15481 gttgggtgac atagccgtaa taacgcgttg gtacattgcg cataaacaag gtcggctgcg
    15541 tcgaaacgta aaatccagca cgcgagacat acatcgcctg ttcaaccatc gacgaggagt
    15601 tgtgcgccag tcgcagcaag taaccaactt ccagtgcagc ggtaatttca ttgtcgaggc
    15661 tttcattttc gcgagacagg aaattcccct cgctgactaa tgcatccgat acgccattga
    15721 ctggcagggt gcgccgtcgg ttgagttcga tttgccaggc gtgctcgtcg cgatgctgtt
    15781 cgaactcggt tacggcatta cgcagagagg tgaaatccag tggcgccacg agagcttcgc
    15841 gcatgccatt acgcaaaaag atcagtttgt cgacgttata ctgcaaatgc ttatcgagcg
    15901 cgttggctac gttttccaga tgattacgct ggctggagat ataggcatct tccagcacca
    15961 ccacttcacg ccaggtgagc aaggtggaaa aaagcaatac cacgataaag cagagattaa
    16021 cgacatgacc gggccccagg cggcgtgcga gttttttcaa ccagctctgg ttttccattt
    16081 ttgtctcgtg ctgcaccctg acgactccca acctgctttt ttatgattct ggcataaccc
    16141 ggcaatagcg ta
//
LOCUS       DAJVQH010000088        15908 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJVQH010000088 DAJVQH010000000
VERSION     DAJVQH010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.088"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(121..825)
                     /locus_tag="OQM87_002841"
     CDS             complement(121..825)
                     /locus_tag="OQM87_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1508909.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(962..1414)
                     /gene="moaE"
                     /locus_tag="OQM87_002842"
     CDS             complement(962..1414)
                     /gene="moaE"
                     /locus_tag="OQM87_002842"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCS1508910.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1416..1661)
                     /gene="moaD"
                     /locus_tag="OQM87_002843"
     CDS             complement(1416..1661)
                     /gene="moaD"
                     /locus_tag="OQM87_002843"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCS1508911.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1654..2139)
                     /gene="moaC"
                     /locus_tag="OQM87_002844"
     CDS             complement(1654..2139)
                     /gene="moaC"
                     /locus_tag="OQM87_002844"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCS1508912.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2142..2654)
                     /gene="moaB"
                     /locus_tag="OQM87_002845"
     CDS             complement(2142..2654)
                     /gene="moaB"
                     /locus_tag="OQM87_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCS1508913.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2676..3665)
                     /gene="moaA"
                     /locus_tag="OQM87_002846"
     CDS             complement(2676..3665)
                     /gene="moaA"
                     /locus_tag="OQM87_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCS1508914.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3653..3797)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4062..4970
                     /gene="yvcK"
                     /locus_tag="OQM87_002847"
     CDS             4062..4970
                     /gene="yvcK"
                     /locus_tag="OQM87_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCS1508915.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5162..7183)
                     /gene="uvrB"
                     /locus_tag="OQM87_002848"
     CDS             complement(5162..7183)
                     /gene="uvrB"
                     /locus_tag="OQM87_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCS1508916.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7762..8439)
                     /gene="bioD"
                     /locus_tag="OQM87_002849"
     CDS             complement(7762..8439)
                     /gene="bioD"
                     /locus_tag="OQM87_002849"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1508917.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8432..9187)
                     /gene="bioC"
                     /locus_tag="OQM87_002850"
     CDS             complement(8432..9187)
                     /gene="bioC"
                     /locus_tag="OQM87_002850"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCS1508918.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9174..10328)
                     /gene="bioF"
                     /locus_tag="OQM87_002851"
     CDS             complement(9174..10328)
                     /gene="bioF"
                     /locus_tag="OQM87_002851"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCS1508919.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10325..11365)
                     /gene="bioB"
                     /locus_tag="OQM87_002852"
     CDS             complement(10325..11365)
                     /gene="bioB"
                     /locus_tag="OQM87_002852"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCS1508920.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11452..12741
                     /gene="bioA"
                     /locus_tag="OQM87_002853"
     CDS             11452..12741
                     /gene="bioA"
                     /locus_tag="OQM87_002853"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCS1508921.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12719..13189
                     /locus_tag="OQM87_002854"
     CDS             12719..13189
                     /locus_tag="OQM87_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCS1508922.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            13743..14744
                     /locus_tag="OQM87_002855"
     CDS             13743..14744
                     /locus_tag="OQM87_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1508923.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(14778..>15908)
                     /locus_tag="OQM87_002856"
     CDS             complement(14778..>15908)
                     /locus_tag="OQM87_002856"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCS1508924.1"
                     /translation="DTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVM
                     VNEVETAYLMQGLWHSKFIACQAGTCRVGLHFLGQCVSVSVSSCHFSRGNYSADESFG
                     IRIQPQTYAWSSEAVRSEAIILDSETMCIGFKNAVYVHDCLDLHMEQLDLDYCGSTGV
                     VIENVNGGFSFSNSWIAADADGTEQFTGIYFRTPTSTQSHKIVSGVHINTANKNTAAN
                     NQSIAIEQSAIFVFVSGCTLTGDEWAVNIVDINECVSFDKCIFNKPLRYLRSGGVSVT
                     DCYLAGITEVQKPEGRYNTYRGCSGVPSVNGIINVPVAVGATSGSAAIPNPGNLTYRV
                     RSLFGDPASSGDKVSVSVVTINVTRPSPVGVALPSMVEYLAI"
BASE COUNT         3766 a         4204 c         3978 g         3960 t
ORIGIN      
        1 gatacggaaa acgattccgc tcgctcataa ggcatatttt atacctggta cttgttaagt
       61 gtcgttttta gtaagaaaaa aatcccgaca catggccggg atagtgatgg tgaaaaagaa
      121 ttaacggcgg ttgccgaaga tccgtaacaa catcaggaac aggttgatga agtccagata
      181 caaggttaac gcgccaagaa tggaatattt gcgcaggttc gacgtgtcgc gggtatcaat
      241 ctgctcaccc atatttttca atttctgagt gtcatacgcc gtcaatccga caaagacaat
      301 cacgccgatg taggtaactg cccacatcaa tgcttcgctt ttcagccaga agttgaccag
      361 cgatgccagc acaatgccga ttaacgccat aaacagcata ttgcccaagc cacttaaatc
      421 gcgcttcgtg gtgtaaccgt acaggctcat tgcgccgaac atcccggcag taacgacgaa
      481 agtactggcg atagaagcag cggtatagac aatgaatata ctggaaagcg taagacccgt
      541 cagcgccgaa taaagcataa agagcatcgt cgttacacct gcgctcagct tttgaatcat
      601 cgctgataac acaataacca atgctaattg cgcgatgatc agaccgatta aaaagacacg
      661 gttagtgaac aacagctcca tcacggccgc ggaattagcc gcataccagg caacaaatgc
      721 ggtcagcaac aagccaacgg tcatccagcc atagacttgc gccatataag tttgcaagcc
      781 agcccggggt tgtacgattg aatcagaacg tgggaatctg tccatgacga tctcctgaag
      841 atataaggaa tatcttaagg atactgcaaa atgatgaggc tgtgcatcga cgcagcgtaa
      901 acgcatgtac tgagcggtga aattgccgga cgcagcggtg ccttatccgg ctaacaaaaa
      961 actaccagcg ttttgccgcc tgcagatcgc tctcccgggc ttcaacccag cggtcgcctt
     1021 ccggcgtggc ttcgcgcttc cagaacggtg cgcgggtttt gagataatcc ataataaact
     1081 gcccggcttc aaacgcactg ctgcgatgcg cactggtgac accgacaaaa acgatttcat
     1141 cgcccggcca taattccccg atgcggtgaa tcaccgtgac gcgccccagc ggccagcggt
     1201 tacgcgcttc atcaacaatt tctgccagtg ctttttcagt catccccgga tagtgttcga
     1261 gggttaatgc tttgacgctg tcgcccaggt tatggttgcg cacctttccg gtgaaggtga
     1321 ctaccgcacc gtcttcgtca cgctccgcca gccacgggta ctcttctcct acgctgaacg
     1381 gctgcgggcc gacaacaatt ttggtttctg ccatcttaac ctccggttac cggcgggagg
     1441 aaagctactt cgtcgccgtc agtcagcgga tggtcaaaac tcaccagcgt ctggttgacg
     1501 gcagccagca atttgccatc ttccagcgcc agcgcccagc gatcgctctg cgcagccagg
     1561 tgctggcgta acgcttcaac ggttgggtaa tccgcagcca cttcggttgc atctgttccc
     1621 accaactcgc gcacctgggc gaaaaaaaga actttaatca tccgcttcca ccttaaagtc
     1681 acccgacttg ccgccgctct tcgccagcaa acgtaccgga ccaatcacca tatctttttg
     1741 caccgctttg cacatgtcat aaatggtcag cgccgccacg gaggccgcgg ttaacgcttc
     1801 catttcgaca ccggttttcc cggtcaggcg gcataaggtt tctatacgca cccgattgtg
     1861 ctccggctcg gcctgtaaat tgacttcaac tttgctgagc atcagcgggt gacacagcgg
     1921 gatcagatcc caagtgcgtt ttgccgcctg aataccggca atacgcgcag tggcaaatac
     1981 gtcgcctttg tggtggcgac catcaataat catcgccagc gtctcgctgc gcatggtgac
     2041 aaaggcttcc gcccgcgctt cacgcacggt ttccgctttg gcggagacat ccaccatgtg
     2101 cgcttcgcca gcggcgttga tatgggtcag ttgcgacata cttatttctt caaatgtgga
     2161 tggaaattac acggacgcgt acgggcatcc agctgcggcg cgatgatatt ttcccatgcg
     2221 gtacggcacg ctttggtcga acccggcatg gcgaaaatca gcgttttgtt agcgacgccc
     2281 gctaccgcac gagattgcaa cgtggaagtg ccaatctctt caaacgacaa catacggaac
     2341 acttcaccaa aaccttcaac ctcacggtcg aacaacggca gcagcgcttc gggagcctga
     2401 tcaccttccg tcaggccagt accacccgta atcaatacca cttgtacatc gtcgctggcg
     2461 atccacgcag atacctgagc gcgaatagcg tagcggtttt ctttcacaat ggctttatcg
     2521 acaacgtgat ggcccgcttc ttgcgccgaa tcgcgcagat agtgaccgga ggtatcgtct
     2581 tcttcaccgc gacgattaga aaccgtaaga atagcaatac gggtcgggat aaattcagtg
     2641 cttacctgac tcatctgatc tctccttttg acgttttagc cgccaatgta cgataagttt
     2701 tgcgtaatac cggtgttgtt ttgatgcagg aaatgggtct gtttcttctc ccgcagcgcc
     2761 gctgaaatac gcgcttccag cgcctgttgc tgggtatcgt cttccagcag atcgcgcagg
     2821 ttaacgccgc cttcaccaaa caggcagaga tggagtttac caatggagga aacgcgcagg
     2881 cggttgcaag tggcgcagaa gtctttttca tacggcatga taaggccaat ctctccggcg
     2941 taatctggat ggcaaaagac ttgcgcggga ccgtcgctgc gttgacgtaa ttggtggatc
     3001 cagccgcgac gcagtagctc gtcacgcaga acctgaccag agatgtgatg cttacggaag
     3061 agctcgctgc cctcgcccgt ttccatcagt tcgatgaaac gcagctggat agggcgatgc
     3121 tggatccagt tcagaaaggt gtcgagctgg tgatgattaa catcacgcat cagcacggta
     3181 ttgactttga ccttctcaaa accggcctca aatgcagcat caatccctgc catgacctgg
     3241 ttgaatttat cctgcccggt aatagcgtga aactggcggg cgtccaaact gtcgacactg
     3301 acgttaatgc cagtaagtcc cgcatcgcgc cagttcgcca catcgcgttc cagacggtaa
     3361 ccattggtgg tgaccgcaat ctggcggata gcgtcgtttt cccgcacagc ggcgatgata
     3421 tcggtaaagt cgcggcgtaa agacggctct cctcctgtca ggcgcacttt ttcggtgccc
     3481 aggctggcga aggcgcgcgt aacccggcga atttcatcga cggtaagaaa gcctttattg
     3541 gtgacgccgc tcggtttgta gccatccggc aggcagtagg tgcaacgaaa gttacacaca
     3601 tcggtaatcg acaggcgcaa gtagtaaaac ttacgcgcaa atgcatcagt cagttgtgaa
     3661 gccatgtaca cctttccaga tacgggaggc gaagtcattt cttccttcgc cctggtggca
     3721 atctttacga gcattgtcac ggccaaagca ccgtatcagt tgacccaggt gcagaggcta
     3781 gagtgtttag tggttaagcc gatactagcg tgtaaatgag atttttacca tccacacttt
     3841 cgctatgcaa tcatgtatat agcgtcatga tcgtgctatt tcgctacaga ataagggaaa
     3901 atggcggttt tactttgata tacatcatca gggaggcata gcgtgcatcg tcctttagag
     3961 atagaagaaa atagtttgta tcctcaatat tggcaggtta attgctgttt ccccgcaatt
     4021 tgcgctactg tagcgcggat aatctgactc caggaaacta tatgcgcaat cgtacgctgg
     4081 ctgatcttga tcgtgtcgtt gctctcggcg gagggcatgg actgggacgc gttctctcat
     4141 cactttcgtc tttgggttct cgtttaacgg gtatcgtcac caccacggat aatggtggct
     4201 cgacggggcg tattcgccgt tcagaaggcg gcattgcttg gggcgatatg cgcaactgcc
     4261 tcaaccagct gataacggaa ccgagcgtcg cctccgcgat gtttgaatac cgttttggcg
     4321 gcaatggcga actttccggt cataatctcg gaaacttgat gttaaaggcg ctggatcacc
     4381 ttagcgtgcg gcctctggaa gccatcaatt taattcgtaa tctgctgaaa gtggatacgc
     4441 atttgattcc aatgtcagag catcctgttg atctgatggc gattgacgat caggggcatg
     4501 aagtttacgg cgaggtcaat atcgaccagt taactacgcc gattcaagag ttattgttaa
     4561 cgcctaatgt acccgcaacg cgtgaggcgg ttcacgctat caatgaagcg gatctcatca
     4621 ttattgggcc tggcagtttt tataccagcc tgatgccaat tctgctgctg aaggaaatcg
     4681 cccaggcatt acgccgcacg ccagcgccga tggtttatat cggcaatctg gggcgtgagt
     4741 tgagtttacc tgcggctaat ttgaagctgg aaagcaagct ggcaattatg gagcagtatg
     4801 ttggtaaaaa ggtcattgat gcggtcatcg tcgggccaaa agtggatgtc tcggcggtga
     4861 aagagcggat tgtgatccag gaggtactgg aggccagcga tattccgtat cgtcatgacc
     4921 gccagttgtt acataacgcg ctggaaaagg cgttacaggc tttaggttaa cggaaatccg
     4981 aaggaaaatt ccggcttcct attgaagaca aagtgcgcgt tgtttatgcc ggatgcggcg
     5041 tgaacgcctt atccggccta caaaccgcgc aaattcaata tattgcggag aaaatgtagg
     5101 cctgataagc gtagcgcatc aggctgtttt ccgtttgtca tcagtcttct tcgctatcct
     5161 gttacgatgc ggcaataaac aactcacgca actgatgcaa ctggtcacga atttgtgccg
     5221 cttcttcgaa ctccagattc tgcgcgtgtt gcatcatcag cccttccagt tcatggattt
     5281 tctgctgcaa cgctttcggc gacatatcca tcggcacatt atccggctca acaatcgggc
     5341 gcgattttcc tctgcccttc gctttggttt tggcaatgtt ctgccccagc gccaggatat
     5401 cgaccacttt cttgttcaag ccttgcggcg taatgccgtg ttcctcgttg tacttctgct
     5461 gtttctcacg gcgacgttcg gtttcgccaa tcgctttcgc cattgatgga gtgatcttat
     5521 cgccgtagag aatcgcttta ccgttaacgt tacgtgccgc acgaccaatg gtctggatca
     5581 acgaacgttc ggaacgcagg aagccttctt tgtcagcgtc gaggatcgcc accagcgaca
     5641 cttccggcat atccagacct tcacgcagta agttgatccc taccaatacg tcgaactcac
     5701 ccagacgcaa gtcgcggata atctccatac gctcgacggt gtcgatatct gagtgaagat
     5761 aacgcacgcg ctcaccgtgt tcttcgagat attcggtaag atcttccgcc attcgcttgg
     5821 tcagtgtggt gaccagcacg cgttcgttaa ttgccgctcg ctgacgaatc tccgaaagaa
     5881 gatcatcaac ctgtgtcgcc accggccgca cttcgataat cgggtcaagc aatccggttg
     5941 gacgcaccac ctgatccacc acatcgccgc cggatttttc cagctcgtaa ttacccggcg
     6001 tcgccgaaac atagatggtt tgcggcgcta atgcttcgaa ctcttcaaac ttaagcggac
     6061 ggttatccag cgctgatggc aggcggaagc cgtactccac cagtgtctct ttacgcgccc
     6121 ggtcaccgcg atacatgccg ccaatttgtg gaatggtgac gtgagattca tcgacgacca
     6181 gcagcccatc ggcaggcagg taatcaaaca gcgtcggcgg tggctcaccc ggtccacgac
     6241 cggagaggaa gcgcgagtag ttttcaatcc ccgaacagta gcccagctcg ttcatcatct
     6301 ccagatcaaa ctgggtacgc tgggtcagcc gctgctcttc cagcagtttg ttgttttcca
     6361 acagcacttt gcgtctggcg gccagttctt ctttgatctc ctccatcgcc tgtacgatgc
     6421 gctcgcgcgg tgtgacgtag tgcgttttcg ggtagatggt aaaacgtgga atagtggaaa
     6481 caatctgccc ggtcagcggg tcaaataacg acaatcgttc cacttcctcg tcaaacagtt
     6541 ccacgcgaag tgcaatgtca tccgattctg ccgggaagat atctatcacc tcgccacgaa
     6601 cgcggaaagt accacgctgg aatgcttgat cattacgagc gtattgcagc tccgccagtc
     6661 ggcgcagaat cgcgcgctga tcgataatca taccgaccgt gagatggagc atcatcttga
     6721 gatataaatc aggatcgccc agaccataaa tcgcggaaac agacgccact acaatcacat
     6781 cacgccgctc cagcatcgct ttggtggcgg acaaacgcat ctgctcaata tgttcgttaa
     6841 ccgaggcatc tttctcaatg aaggtgtcgg aactcggtac ataggcttcc ggctgatagt
     6901 agtcgtagta ggagacgaaa tactccaccg cgttttccgg gaagaactct ttcatttcgc
     6961 catacagctg ggccgccagc gttttgttgg gcgcaagtac catggttggg cgctgaaggt
     7021 cagcaatgac attggcaatg gtgaaggttt tcccggagcc agtcacgcca agtaacgtct
     7081 ggtgcgccag gccatcttcc agcccctctt cgagacgtcg aatcgcctct ggctgatcgc
     7141 cagaaggttt aaaagcggaa ttcagtttga acggtttact catgagtcgc tacctgaagg
     7201 agttgggcgg gcaggtatgt aattttactc gtcgtactta attatgccaa caaattatac
     7261 tggataaaaa aacagttcat caccataata tttctgatac agcgtaaact ccgctgtcaa
     7321 tcatgagcaa aatttactct gtggcgagat aaaactccgg ccttaccggg ttatccccaa
     7381 agcaacggct tttttaacat ttgtcaagat gagtgatgac agttttatgg caagagatgc
     7441 ctgtacagtg actgccattg catttatcta accagttaaa aaataaaaga tatttctttg
     7501 agccgtcttt aacgccaatt cgcgtgcaag ccgcgtattc tctcgcttgc ctcgtgtttt
     7561 ctaactctaa tacacatggt tatccacagg aatagtggat aactgtctcc agcccctatc
     7621 ccccgccgcc tgggcaattc ccacattcgc cgcatgaggc ggaagtaaaa tttttttcgt
     7681 gattatttta gaatttaatt ggttaaattg cagtcaatcg aagacgcaat ctcgctcgca
     7741 atttaaccaa atacagaatg gctacaacaa ggcaaggttt atgtactttc cggttgccgc
     7801 attttctgga ttttctgcaa gccaggggat ctctcccagc agcggcgcgg gaatcatgcg
     7861 ggtgagcgtg gtcatatatt cagcgtgacg ttttcccgga ggcgtaacat cgttcgccac
     7921 ccaacccgcc agagtcagtc cggcgtgttg tattacctgc gcagtcaaca tcgcgtgatt
     7981 aatacagccg agtttcacac caactaccag tatcaccggc agttgttcct gtgttaccca
     8041 atctgcaaaa gtgaaagtgt cagaaagcgg cgtaaaccag ccgccagcac cttccactaa
     8101 cacccagtca gcctgttgtt caagcgcgcg taatccggcg ctcattacca atgattctat
     8161 cggcctgccc tcttgggcgc tgatgatgtg cggcgaggtg ggttctgcga aggtgtaagg
     8221 atttaccgtt gcgtaatcca gctgcaggct gctgttgtgc tgtaacgcca gcgcgtcact
     8281 attacgtaag ccttccgggg tcttttcgct gccagaggcg accggtttat aacctgccgt
     8341 ccgataacct actgccttcg cggcttgtaa aagtgcacaa ctggcgacgg ttttccccac
     8401 ttcggtatcc gttccggtga caaaataacg tttactcacg agcaatcact cccaaaaaaa
     8461 gatgatacgt cagaggatat cgcccctgtt gttgcggcca ggccagttgc aatcgctgca
     8521 actgcgaacg cgttaatatt cgcgggtcgc gcccttcatg aagatgcgta gcaccgatgc
     8581 ctttcagcga acgcatggca ctgagcgcat catcaaacca tagtgtgatg agctgaatat
     8641 gatgttgata acgcacgccg ttcagcgact cttcgatttc atctggcggt aaaaagcgat
     8701 tagcatgcgg acgctcgtcc accgcctgcc gcgcctgatg cagttcgggt aacgatccct
     8761 gcaccagcgt ggtaaacgcg accacgcctc cggggcgcac cacccgatac agctcgcgga
     8821 gtgccgtgga taaattaccg caccactgca ccgcgagatt gctccatgca agatcgaacg
     8881 tcgcagtcgc taacggcagg gattcgatat ctcccgccag ataatgatct gcggcatctt
     8941 tatggcgggc ttgcgcaagc attggcggcg agagatctaa cgccgtcacc tgcgcgtgac
     9001 gttcccgcca gtggcggctc atccagccag gtccacaacc cgcgtccagt acgtgggtgt
     9061 atttacgctg tggaagcatt gccagtaagg cgtcagcact ctggcgctgt agatctgcat
     9121 gttgctcata gtgtgcggct gcccgaccaa atgccgctgc aatggcttgt ttattaaccg
     9181 ttgccatgca gcacctccag cagacggtcg atatcctgca tttcatgcgc agcggttagc
     9241 gttaagcgaa gtcgcgcagt accagcgggt acggttggcg ggcgaatcgc cgtgacccag
     9301 cagccttgct ggcgcagttt ttctgccagt tgtaacgcac ggctgttatc accgacgatc
     9361 aatggctgga tggcgctgca tgaatcagcc agcgtaaacg gcaaatcctg tactccggca
     9421 cgaaaacgcg caatgagtga caccagtttt tcgcgccgtg catccccctc atcacggcga
     9481 atgaccgcca gcgacgcacg taatgcctgc gcctgagcgg gcggcatact ggtgctgtag
     9541 ataaggtggc gggcgaattg cagcagataa tccgccaccg tacttgagca aagcaccgca
     9601 gccccactga cgccaaatcc tttgccaaaa gtcactacca gcaattctgg ttttaccttt
     9661 tgcagccagc agctgccgcg cccctgctcc ccgataacgc ccgtgccgtg ggcatcatcg
     9721 accatcaacc agccattgtg ctgttgcgtt acctgctgga tttccgccag tggcgcgcta
     9781 tcgccgtcca tgctgaacac gccttctgtc accaccagtt gctgccccgg acagggggaa
     9841 gcaagcagtc gcgccagatg agcgacatcg ttatgagtaa aacggcgaag ctgcgacggg
     9901 cttaaactgg cagcctccag caatgaggca tggctaagcc ggtcggcaac aatacggtcc
     9961 tctttcgcca tcatcgcggc aatgactgcc tgattcgcgg caaaaccaga gataaacagc
    10021 agcgcccgcg aatagccaag ccactcggcc agttcttctt ccagcgcctg atgcaccacg
    10081 ctataaccgc tgacgtgacc ggagccgccg ctaccgatgc caaattgctc cgccccctgc
    10141 ttccaggcac ggataatttg cggatgatgg cttaaaccta aataatcgtt actggaaaag
    10201 ttcagatact ggcgatcatc cgccaccagc cagcgtccgg ctccttgcgc caccggataa
    10261 cggcgacgca gggcatcggc agcacgccgc gcatcgagcg ccgcgttgat tttctcctgc
    10321 cagctcataa tgctgccgcg ttgtaatatt cgtcggtgtc cggggtcatc agcgcctgtc
    10381 caagacgttg ctgttgttcg ttatcccctg ccagcacggc agtttgctgc ggatttagcc
    10441 ccagtttgcg gaacagttgc aggtctttat cttcttccgg attcggcgtg gtcagcagtt
    10501 tgcaaccgta gaaaatcgag tttgcgcctg ccataaagca catcgcctga gtctgttcgt
    10561 tcatctgctc gcgtccggca gaaaggcgca cgtaagaggt cggcatcatg atccgcgcta
    10621 cggcaatggt gcgaataaaa tcaaaggcat caacgtcatc gttatcggca agcggcgtgc
    10681 ctttcacctt caccagcatg ttgattggta cgctttccgg cggcgtcggc aggtttgcca
    10741 gttgcagcaa taatccggcg cgatctttta ccgtttcgcc taagcccaca atgccacctg
    10801 agcagacttt gatcccggca tcgcgcactt tttccagcgt atcgaggcgt tcctgataag
    10861 tgcgtgtggt gatgatattg ccgtaaaact ccggcgaggt gtccaggttg tggttgtagt
    10921 aatccagccc ggcgttcgcg aggcgctgcg cctgagattc actcaacgtg cccagcgtca
    10981 tacacgcctc cagccccatc gcttttaccc cctgcaccat ttgttccagg tacggcatat
    11041 cgcgttcgtg gggattcttc catgccgcgc ccatacagaa gcgcgtcgat cctgccgctt
    11101 tcgctttgcg cgccgactcc agcacctgtt caacttccat caaccgctcg gcttccagcc
    11161 cggttttgta gcgcgagctt tgcgggcagt atttgcaatc ttccggacaa gctccggtct
    11221 taatcgacag caacgtgctg acctgcacct gacgaggatc gaaatgctgg cgatgcacct
    11281 gctgcgcttc aaacagcaga tccagcaacg gtttttcaaa taattctgtg acttgcgaca
    11341 atgtccagcg tgggcggtga gccatggggc ttctccaaaa cgtgtttttt gttgttaatt
    11401 cggtgtaggc ttgtaaacct aaatcttttc aatttggttt acaagtcgat tatgacaacg
    11461 gacgatcttg cctttgacca acgccatatc tggcacccat acacatccat gacctcccct
    11521 ctgccggttt atccggtggc gagcgccgaa ggttgcgagc taattttgtc tgacggcaga
    11581 cgcctggttg acggtatgtc gtcctggtgg gcggcgatcc acggctacaa tcacccgcag
    11641 cttaatgcgg cgatgaagac gcaaattgat gccatgtcgc atgtgatgtt tggcggtatc
    11701 acccatgcgc ccgccatcga gctatgccgc aaactggtgg caatgacgcc gcaaccgctg
    11761 gagtgcgttt ttctcgcgga ctccggttcc gtagcggtgg aagtggcgat gaaaatggcg
    11821 ttgcagtact ggcaagccaa aggcgaagcg cgccagcgtt ttctgacctt ccgcaatggt
    11881 tatcatggcg atacctttgg cgcgatgtcg gtgtgcgatc cggacaactc aatgcacagc
    11941 ctgtggaaag gctatctgcc agaaaacttg tttgctcccg ccccgcaaag ccgcatggat
    12001 ggtgaatggg atgaacgcga tatggttggt tttgcccgcc tgatggcggc gcatcgtcat
    12061 gaaatcgcag cggtgatcat tgagccgatt gtccagggcg caggcgggat gcgcatgtac
    12121 catccggaat ggttaaaacg aatccgcaaa atgtgcgatc gcgaaggtat cttgctgatt
    12181 gccgacgaga tcgccaccgg atttggtcgt accggcaaac tgtttgcctg tgaatatgca
    12241 gaaatcgcgc cggacatttt gtgcctcggt aaagccttaa ccggcggcac aatgaccctt
    12301 tccgccacac ttaccacgcg cgaggttgca gaaaccatca gtaacggcga agccggctgc
    12361 tttatgcatg ggccaacttt tatgggcaat ccgctggcct gcgcggcagc aaacgccagc
    12421 ctggcgatta tcgaatccgg cgaatggcag cagcaggtgg cggctattga agtgcagctg
    12481 cgcgagcaac tggcaccagc ccgtgatgcc gaaatggttg ccgatgtgcg cgtactgggg
    12541 gcaatcggtg tggtcgaaac cactcgtccg gtgaatatgg cggcgctgca aaaattcttt
    12601 gtcgaacagg gggtatggat ccggcctttt ggcaaactga tttacctgat gccgccctat
    12661 attattctcc cgcaacagtt gcagcgtctg accgcagcgg ttaaccgcgc ggtacaggat
    12721 gaaacatttt tttgccaata atggcgataa gttgccgcat cgtcatgtct ttaacggcat
    12781 gggttacgat ggcgataata tttcaccgca tctggcgtgg gatgatgttc ctgcgggaac
    12841 gaaaagtttt gttgtcacct gctacgaccc ggatgcgcca accggctccg gctggtggca
    12901 ctgggtagtt gttaatttac ccgctgatac ccgcgtatta ccgcaagggt ttggctctgg
    12961 tctggtagca atgccagacg gcgttttgca gacgcgtacc gattttggta aaaccggata
    13021 cgatggcgca gcgccgccga aaggcgaaac ccatcgctac atttttaccg ttcacgcgct
    13081 ggatgtagag cgtattgatg tcgatgaagg aggcagcggc gcgatggtcg ggtttaacgt
    13141 tcatttccac tctctggcaa gcgcatcgat taccgcgatg tttagttaat cactctgcca
    13201 gatggcgcaa tgccatctgg tatcacttaa aggtattaaa aacaactttt tgtcttttta
    13261 ccttcccgtt tcgctcaagt tagtataaaa aagctgaacg agaaacataa aacgctgtaa
    13321 atatcaatat gttatgaaag atttagtcta aataatagac tgcataatac tacaaaacac
    13381 aacatatcca gtcactatga atcaactact tagatagtat tagtgacctg aggcagagca
    13441 ttagcgcaag gtgatttttt gtcctcttgc gctaattttt tgtcaacgga ctgggttagt
    13501 tgaatttaca accatgctaa gtatctcgat aagcgcagag aaatgatgca gtggtgggcg
    13561 gattggcttg atgagaaggt taggtaggca aatccatgcc tgagcagagc cttgccctct
    13621 acctgagccc atgctcattc tttggcctca acgcgagcat gacctcactg gctggaccca
    13681 aaatggacta tgacagaaag atctgatatt tttcctcgca aaaacttaga ggctttattt
    13741 taatgcataa gagcaactgc tttgacacgg caaggcttgt ggcagctatg atggttctgg
    13801 tcagtcacca ctacgcgctc tctgggcagc cggagccgta cctgttcggc tttgaaagtg
    13861 ctggcggcat tgcagttatc atattctttt caatatcagg atatctgatt tcaaaatcag
    13921 ctatcaggag tgattccttt attgatttca tggctaaaag agcgagaaga atattcccgg
    13981 cgctggtccc ttgctctata ctgacatatt ttttatttgg atggatactt aacgacttca
    14041 gcgcagaata ttttagccat gacattgtca ggaagaccat aagctctatt tttatgagtc
    14101 aggctccaga tgctgacatt acatcacatt taattcatgc cggaattaac ggcagtttgt
    14161 ggaccctacc gcttgagttc ctgtgttata taataacggg agtagcagtt gccctactaa
    14221 agaacggaaa agcattcata gttatactgt tggtttttgt ttctctttca ttaattggaa
    14281 gtgtgtcaga aaacagggat gtaatgttct ctattccgtt atggctgtat cctttgcgtg
    14341 gactggcgtt cttttttggt gccactatgg caatgtatga gaagtcatgg aatgtaagta
    14401 atgttaaaat tactgtagtt tctttgcttg ctatgtacgc ctacgctagt tatggcaaag
    14461 gcattgatta tactatgact tgctatatcc tggtttcatt ctcgacaata gccatatgca
    14521 catccgttgg cgacccactg gttaagggca ggtttgatta ctcgtatggt gtgtacattt
    14581 atgcattccc ggttcagcag gttgttataa acacattgca catgggtttt tacccgtcaa
    14641 tgttgttaag tgccgtgact gttcttttct tgtcccatct ttcatggaat ctggttgaaa
    14701 aaagattcct gacacgaagt tcgcctaaat tatccctgga ttgaacaata aaaggggcca
    14761 tatggcccct aaaataatca gatggccaga tactcaacca ttgaaggcag cgcgactcct
    14821 actgggcttg ggcgagtaac attaattgtc accacggaaa cactaacctt gtcaccactt
    14881 gatgcagggt caccaaaaag gcttcttact ctgtatgtca ggttccccgg gttcggtata
    14941 gctgcagatc cgcttgtggc acctaccgca actgggacat taatgatccc atttacagac
    15001 ggtacgcctg aacagccacg atacgtattg tatctgcctt ccggtttctg cacctcggta
    15061 atgccagcca aataacagtc agtaactgac acgccaccgc tacgaagata gcgtagaggc
    15121 ttattgaata tgcacttatc gaaagaaaca cattcattga tgtcgacaat atttacagcc
    15181 cattcatcac cagttaacgt acaaccactt acaaagacga agatcgccga ctgttctatc
    15241 gctatacttt gattgtttgc agccgtgttt ttgtttgcag tgttgatatg aacaccactg
    15301 acaattttat gtgactgcgt tgaggtcggc gttctaaaat atatccccgt aaattgttca
    15361 gtgccatcgg catctgctgc tatccatgag tttgagaaag aaaatcctcc gtttacattc
    15421 tctataacca cgcctgttga accgcaataa tctaaatcca gttgttccat atgcaaatca
    15481 aggcaatcat gaacatagac ggcattttta aaaccaatgc acatggtctc actgtctaaa
    15541 attattgctt ctgacctaac tgcctctgac gaccacgcat acgtttgagg ctgaatcctg
    15601 atgccaaagc tttcatcagc agaataattt cctctgctga aatggcagga gctgacacta
    15661 acacttacgc actggcctaa aaagtgaaga ccgactctac aggttccagc ctgacatgct
    15721 ataaattttg agtgccagag cccttgcatt aaataagcgg tttcaacttc attcaccatt
    15781 acattatcaa atacccagcc ataaacctgt ctgatgacag agtcttctgc tgtaatgttt
    15841 gtgtcaatac cattgcaggt gttattacca gttactcgta tatttctcag ataacggccg
    15901 atagtgtc
//
LOCUS       DAJVQH010000089        15887 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJVQH010000089 DAJVQH010000000
VERSION     DAJVQH010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.089"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..1896)
                     /gene="rnr"
                     /locus_tag="OQM87_002857"
     CDS             complement(<1..1896)
                     /gene="rnr"
                     /locus_tag="OQM87_002857"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCS1508925.1"
                     /translation="MSQDPFQGREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVAD"
     gene            complement(1935..2360)
                     /gene="nsrR"
                     /locus_tag="OQM87_002858"
     CDS             complement(1935..2360)
                     /gene="nsrR"
                     /locus_tag="OQM87_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCS1508926.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            complement(2565..3863)
                     /gene="purA"
                     /locus_tag="OQM87_002859"
     CDS             complement(2565..3863)
                     /gene="purA"
                     /locus_tag="OQM87_002859"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCS1508927.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(3967..4164)
                     /locus_tag="OQM87_002860"
     CDS             complement(3967..4164)
                     /locus_tag="OQM87_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCS1508928.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(4246..5250)
                     /gene="hflC"
                     /locus_tag="OQM87_002861"
     CDS             complement(4246..5250)
                     /gene="hflC"
                     /locus_tag="OQM87_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCS1508929.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(5253..6512)
                     /gene="hflK"
                     /locus_tag="OQM87_002862"
     CDS             complement(5253..6512)
                     /gene="hflK"
                     /locus_tag="OQM87_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCS1508930.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(6598..7878)
                     /gene="hflX"
                     /locus_tag="OQM87_002863"
     CDS             complement(6598..7878)
                     /gene="hflX"
                     /locus_tag="OQM87_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCS1508931.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(7954..8262)
                     /gene="hfq"
                     /locus_tag="OQM87_002864"
     CDS             complement(7954..8262)
                     /gene="hfq"
                     /locus_tag="OQM87_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCS1508932.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(8348..9298)
                     /gene="miaA"
                     /locus_tag="OQM87_002865"
     CDS             complement(8348..9298)
                     /gene="miaA"
                     /locus_tag="OQM87_002865"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCS1508933.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(9291..11138)
                     /gene="mutL"
                     /locus_tag="OQM87_002866"
     CDS             complement(9291..11138)
                     /gene="mutL"
                     /locus_tag="OQM87_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCS1508934.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(11148..12485)
                     /gene="amiB"
                     /locus_tag="OQM87_002867"
     CDS             complement(11148..12485)
                     /gene="amiB"
                     /locus_tag="OQM87_002867"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCS1508935.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(12504..12965)
                     /gene="tsaE"
                     /locus_tag="OQM87_002868"
     CDS             complement(12504..12965)
                     /gene="tsaE"
                     /locus_tag="OQM87_002868"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCS1508936.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(12937..14484)
                     /gene="nnr"
                     /locus_tag="OQM87_002869"
     CDS             complement(12937..14484)
                     /gene="nnr"
                     /locus_tag="OQM87_002869"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCS1508937.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            14483..15622
                     /gene="queG"
                     /locus_tag="OQM87_002870"
     CDS             14483..15622
                     /gene="queG"
                     /locus_tag="OQM87_002870"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCS1508938.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(15605..15658)
                     /locus_tag="OQM87_002871"
     CDS             complement(15605..15658)
                     /locus_tag="OQM87_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508939.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3627 a         4528 c         4005 g         3727 t
ORIGIN      
        1 gtcagcgaca tcgcgcgttg cttcgtcggc acgacgttcc gccatcgaac agtgctgacc
       61 cagttgcaac atctcttcca tcgaataatg gtagccgccg gtttcagtgg tgttgccctg
      121 atgcccctgc tctttcgcca gcagatattt aatggcgcgg tgcagcgtca ggtcaggata
      181 acgacgaatc ggcgaagtaa agtgcgcata ggactgcaat gccagaccga agtgaccacg
      241 gttttctgga tcgtaaatcg cctgtttcat cgagcgtagc agcatggttt gcagcatttc
      301 tgcatcagga cggtcggcaa ccgactccag cagttccgcg tagtcacgcg gttccggctt
      361 gttaccaccc ggcagctcca gccccagctc cgccagcact gaacggaaag aggtaatcgc
      421 ttcggtgctc ggcttgtcgt gaatacggaa cagtgccggt tctttcgctt tctcaacgaa
      481 acgcgccgcc gagatattcg ccagaatcat gcactcttca attaatttgt gcgcgtcgtt
      541 acgctgggtc tgttcgatac gttcaatacg gcgttcagcg ttgaaaatga acttcgcttc
      601 ttcgctctca aatgagatcc caccgcgttc ttcacgggct ttatccagca ctttatagag
      661 gttatgcaac tcttcgagat gcttaaccag cggggcgtac tgctcgcgca gatcctgatc
      721 gccctgcaga atatgccaga ctttggtgta ggtcagacgc gcgtgagagc tcatcaccgc
      781 ttcgtagaat ttgtagcccg tcaggcggcc tttcgacgaa accgtcatct cgcacaccat
      841 acacaggcgg tctacctgcg ggttgagcga acacaggccg ttagagagca cttccggcag
      901 catcgggata acctgcgaag ggaagtacac cgacgtgcca cggttacgcg cttctctgtc
      961 cagcggcgtt ggcggacgca catagtagct gacgtcggca atcgcgaccc ataaacgcca
     1021 gccgccgccg cgttttttct cgcagtaaac tgcatcgtca aagtcacggg cgtcttcgcc
     1081 atcaatggtg accagcggta aatcgcgcaa atcgacacgg cccgcttttg cttcttccgg
     1141 cacttcttct ttcagccccg caacctgttg ctcaacagcc tgcggccaga tatacggaat
     1201 ttcatgggta cgcagagcga tatcaaccgc catgccggtg cccatattgt cgcccagcac
     1261 ttcgacgatt ttacccaccg ctttggtgcg gcgagtcgga cgctgagtca gttcgactac
     1321 gaccacaaag cccatccgcg cgcccatgat ctgatcgggc gggattaaga tatcgaagct
     1381 cagacggcta tcgtcaggaa ccacaaagcc gacgcccgct tcggtaaagt agcgaccaac
     1441 aatctggctg gtttttggca ccagtacgcg gacaatacgc gcttcacgac gacctttacg
     1501 gtcagcaccc agcggctgcg ccagcacctg atcgccatga atgcaggttt tcatctgctc
     1561 gctggagaga tacaaatcat ctttacgccc ttcaacccgc agaaagccgt agccatcacg
     1621 gtggccaata acggtacctt tcaccaggtc gaggcgttcc ggcagcgcat agcactgacg
     1681 acgagtgaag accagttgac catcgcgctc catcgcgcgc aggcggcgac gcaggccttc
     1741 aagctgctct tcgccttcaa tgtgcagttc taccgccagc tcatcacggc tggccggttt
     1801 ttcacgtttg gttaaatgtt cgaggataaa ttcccgacta gggatgggat tcgcgtattt
     1861 ttcagcttcg cgtccctgga aaggatcttg tgacatctcg gttcctccgt tgtcatctct
     1921 gatgaagatt ttcgtcactc caacagcaat aatttataaa gcggttgatt ctcttcaacc
     1981 aaatcggcaa gcgtgtagtt atccagttcc gtaagaaaac tttgcacggc cttagaaagc
     2041 gcctgtttca accgacaggc aggtgtaatg tggcaaaact cactgctgca attcaccagc
     2101 gataagggct ccagctcgcg caccacatca ccaatacgta tcgcactcgc cggtttaccc
     2161 aggcgaatgc cgccattttt tccacgaaca gcagtcacgt agccggcacg actaagttga
     2221 ttgattattt tgaccatatg attacgggag acgccgtaga cgtcagtcac ttcagaaata
     2281 ctggtcatcc gcccttctgg caatgacgcc atgtagatca gcgcacgtaa tccgtaatca
     2341 gtgaaactcg ttaactgcac atcaacctca aaagggaaat cgggaaaaaa tacatttata
     2401 ttgatgataa accagccaca agctgtgtcg ctaatttatt tcagttcggg gaaggaaaaa
     2461 agcgaggatt taacactgtg tcggatagcg taatggctta accgacacag caatgtatca
     2521 cactgttagc ccggcaggca aaatatctgc caggcgtacc agaattacgc gtcgaacggg
     2581 tcgcgcagaa tcatggtttc agtacgatcc ggaccggtag agatgatatc gatcggcaca
     2641 ccggtcagct cttcaatacg cttgatgtag ttcagcgccg cctgcggcag gccgctacga
     2701 tctttcacgc cgaaagtgga ttcagaccag cccggcatgg tttcgtaaat cggctctaca
     2761 cctttccagt cgtcagctgc cagcggagtg gtagtcactt cgcgaccatc cggcatacgg
     2821 taagccacgc agagtttaac ctctttcagg ccatccagaa cgtccagttt agtcaggcag
     2881 aagccagaca gggagttcag ctgtaccgca cgacgaacgg caacggtgtc cagccagccg
     2941 gtacgacgac gacgacccgt agttgcaccg aattcgttac cctgcttgca gaggaactcg
     3001 ccagtttcat caaacagttc ggccgggaaa ggacctgcac ctacacgagt tgagtaagct
     3061 ttgaggatac ccagaacgta atcaacataa cgcgggccca ggccggaacc ggtcgccacg
     3121 ccaccagcag tggtgttgga agaagttacg tacggataag taccgtggtc gatatccagc
     3181 agcgtaccct gcgcaccttc gaacatgacg aaatcgccac gctgacgcgc ctggtcgagc
     3241 aggtcagaaa cgtcaaccac catagaagtc aggatgtcgg caacagccat cgtatcatcc
     3301 agaactttct ggtaatcaac cgcttcagct ttgtagtagt taaccaactg gaagttgtga
     3361 tattccatca cttctttcag tttttcagcg aaggtttctt tgtcgaaaag gtcgccaaca
     3421 cgcagaccgc gacgtgccac tttatcttca taagcaggcc cgataccacg accggtggtg
     3481 ccgatcgctt tcgcgccacg cgctttctca cgcgcgttat ccagcgcaac gtgataatca
     3541 aggatcagcg gacatgcttc agacagcagc agacgctcac gaacggggat gccacggtct
     3601 tccagttctt tcatctcttt catcagcgcg gccggagaca gcacaacacc gttaccgatg
     3661 atgctggtta cattctcgcg gagaatacct gatggaataa gatggagaac ggttttttca
     3721 ccgttgatta cgagagtatg gcctgcgttg tgaccgccct ggtagcgtac aacatattta
     3781 gcccgttcag tcagaagatc gacgatctta cctttacctt cgtcacccca ttgggtgccc
     3841 agtacgacga cgttgttacc catttttcaa aatcaccgtt tgcttaaaaa tggattctac
     3901 catcgctttt tcagagttac agcacttttt gcactcaaaa atgacctcga acagtctgtt
     3961 ttttgttcag ccaatcgttt tcctcaacat gtagtagacc acaacgcccg caaccacaag
     4021 tccaccgcca aaacgacgta aaatattatc gggcaaattg gtcatcgcag agatcatctt
     4081 cttccatgcc ttcgggtaaa gcatcggccc taaaccttcc agtaccaaaa ccagggcaag
     4141 cgccagccag attgtcgaat tcattcatta tccttataga aaaagaaaac caccgacatc
     4201 cctgaggatg cggtggcttt attgacctgt accgcagtcg ttatattaac gcgttgcgga
     4261 agtcggcgtc ttcatgtagc ggaagaaatc gctatccggg ctcatgacca tcacgtcctg
     4321 attgccagag aagctgttct cataagcacg caggctacgg atgaatgcgt agaagtccgg
     4381 atctttactg aatgcatcag caaacagttt ggctgcttcg gcatcgcctt caccacgcat
     4441 aatgcggccc tgacgctcag cttctgccag cgttctggtc acttcatagt cggcagtcgc
     4501 gcgcagtttt tccgcttctt cctgaccttg tgaacggtga cgacgcgcta ccgcttcacg
     4561 ctcggcgcgc atacggttgt agatcgcttc agacacttcg gtcggcaggt tgatctgctt
     4621 gatacgcaca tcgacaactt caatacccag cgccgccata ctgttcgggt tgatgaccgg
     4681 aactttgccc ttcgtctcag ccgttacgcg ctctgccgct tcggcaatgg cgttatctgc
     4741 tgccggggta gtaacttcat cttctgtacc cgcagaaccg gagttcagcg cgtcacgtac
     4801 ttcgagggtc agacgaccac gggaatcggt gacgatatct ttcacgtcca ggcgaccaat
     4861 ttcagaacgc agacggtcag agaacttacg tttcaacagc acttccgctt gcgaaatgtc
     4921 gccaccaccc gttgccaggt agtaacggct gaaatcgctg atgcgccatt tgatgtaaga
     4981 gtcgacgatc aggtctttct tctctttggt cacaaagcgg tcggcctggt tgtccatggt
     5041 ctgaatacgt gcgtcgagca ttttcaccgt ttcaatgaac ggtatcttga aatgcagacc
     5101 aggctcataa accaaaggtt tgttgtcatc gtcacgcagt accttaccaa aacgcagcgt
     5161 aataccacgc tcaccttctt tgacgacaaa gacagacatg taaagcacta ccagcacgat
     5221 gatgataatc gcgataactg acttacgcat cgttattccc cctgacgctg gtagtcgtta
     5281 cgctgcgcgt tggcgcggcg ttggtccata atatcgccct gactggtgga cgacgtgttg
     5341 cttgctccac tggttgtgga ggaagaggct ggcggcagac gcagcagatt gctggcaccg
     5401 ttatcgctct tcgccgcagg ggcgttacca cctttcagca tctggtctaa cggcagaacc
     5461 atcaggttgc cacctttatc gttaaccagc actttgcggg tgttacccaa cactttttcc
     5521 atcgtctcga tatacagacg ctcgcgagta atttccggcg cggctttata ttccggcaga
     5581 agtttagcaa agcgcgccac ttcaccctga gcttccagga tggtctgggc cttgtacgca
     5641 cgcgcctctt cgaggatacg ttgcgcctga ccgttcgcac gcggctgaac ttcgttggta
     5701 tacgcttctg cttcacgaat gtattgctgt tcgttttcac gcgcggcaat cgcatcgtca
     5761 aacgccgctt ttacttcttc cggcggacga gcagcctgga agttgacgtc cagcagcgtg
     5821 atacccatgt catacggacg aatcgtctct tccagttcgc gctgagtatc gctacgaatc
     5881 acggtacgac cttccgtcag aatgcggtcc atggtgtatt taccgataac tccacgcagg
     5941 gcgctgtcgg tagcctgacg caggctgtca tccgggctgg tcacgctata cagatatttt
     6001 tccggattgg tgacgcggta ctgcacgttc atctcaacgc gcactacgtt ctcgtccgac
     6061 gtcagcatca caccagaagc ggccagttca cgcacggctt ccacgttcac cggtttgact
     6121 tcgtcgataa acgtcggttt ccagttcaga cccggctcaa ccagatggct gaatttacca
     6181 aagcgtgtta ccacgccgcg ttcggcttct ttaatggtat agaaaccact ggccgcccag
     6241 ataatgacaa tcgctgccgc tgcgatggta acgacacgac cgccaagctg cgggcgcggg
     6301 ccttgcgatg aactgccacc gccagatccg gtgcctttac cgccgcccag accaccgagc
     6361 tttttgctca gtttgcggaa gatatcatct aaatcaggtg gcccttgatc gcgaccgcct
     6421 ttgtttccat ttccctcaga gttgccgcca ggtttgctgc ttccccacgg gtcgcggtct
     6481 tgtccgttat taccgggctg attccacgcc atgtttgtgc tccatatttg ttatgcggtg
     6541 atccctgttg tctttcgacg ccagaggata tgactccacg cttcagacgc tacgccgtta
     6601 gatcaggtaa tcgatcaacg ccggttcttg tttacagagg cgacgccagt caacgatcgg
     6661 catacgaact tgcagactta cgctgccgtc ctcctccatc cactcttttt ctattgcctg
     6721 aagctgataa aaacgactcc tcagacgccc ttcctgcggt ggcagacgca atgtatgctg
     6781 cgccacctcg ccggaaagcc gctccgtcaa agcctgaaaa agctgtggta tccccgctcc
     6841 ggtctgtgcg gaaagccaga cacggatcgg tttgttctct tcgtcccgat caatacgcgg
     6901 ttcgaaatct tccagcatat cgatcttgtt catcaccagc agggttggga tctcgtgagc
     6961 gtcgatctct tcaagaaccg tattcaccgc ttcgatgttt tcttgtacac gcacatccgc
     7021 cgcatcaatg acgtgcagca gtaatgtggc ttgccgcgtc tcttgtaacg tggctttaaa
     7081 tgctgccacc agatcgtgcg gcaggtggcg aataaaccct acggtatctg caagtacggt
     7141 ttcaccgaca tctgcaacgt caatacgccg caacgtcggg tcgagggtgg caaacaactg
     7201 gtctgccgcg tagacccgcg cttcggtgat gcgattgaaa agggtagatt taccggcgtt
     7261 ggtatatccc accagcgaaa cagtaggaac gtcggctttg atacgcgatt gccgcccctg
     7321 ctcacgctgc ttttcaactc tttccaggcg cgactgtatc tgcacgatgc gattacgcaa
     7381 caaacgacgg tcggtttcga gctgggtttc acccggacca cgcaaaccta tcccgccttt
     7441 ctgtctttca aggtgggtcc agccacgcac caggcgcgta gccagatggc gcagctgcgc
     7501 cagctcaacc tgcaacttac cctcatgggt acgcgcacgt tgggcgaaaa tatctaaaat
     7561 aaggccggtg cggtcgataa cacgacactc gcacaaacgc tccaggttac gctcttgcgc
     7621 cgggctcagg gcatggtcaa aaagaacgac cgaagcaccc gtcgctttga cagcttccgc
     7681 aatttcaact gctttacctt cacctacaaa atactttggg tgcggcgctt tacggctacc
     7741 ggtaatcacc tgcaatgctt cgacaccggc ggaagagacc agagattcaa actcctggag
     7801 gtcttccata tctttgtctt gcgtaaaata gatgtgtacc agtaccgcct gctcaccagc
     7861 atcataacgg tcaaacaagc gtataaccct ctaaatagat cagcggggaa cgcaggatcg
     7921 ctggctcccc gtgtaaaaaa acagcccgaa accttattcg gtttcttcgc tgtcctgttg
     7981 cgcggaagta ttctgtgcgc tgctaccatg atggtagtta ctgctggtac cgccaccggc
     8041 gttgttactg tgatgagaaa ccgggcgaga tgggacaaca gtagaaatcg cgtgcttgta
     8101 aaccatctgg ctgaccgtgt ttttcaacag gatcacgaac tgatcaaaag actcgatttg
     8161 cccttgcagc ttaataccat tcaccaaata aatagaaact ggaacacgtt cccgacgcag
     8221 tgcgttcagg aacggatctt gtaaagattg ccccttagcc attctctctt ttccttatat
     8281 gcttatttgt actttgaacc tttcgattct gaaaaaattg cgcacgatac gtctcaattg
     8341 tacacattca gcctgcgata gcaccaacaa cctgtaatac ttcgtcacgc gcctgttctg
     8401 gtttttcact gtcaagccag tgaacccctt cccaaccacg cagccaggtt atctgccgct
     8461 tcgccaactg tctcgtggcg caaacacctc gataaaccat ttcatcgtat gagatttcgc
     8521 cttcaaggta agaccacatc tggcgataac ccacgcaacg aatggaaggc aagtccgtat
     8581 gcaaatctcc tcgggcaaaa agcgcccgga cttctgcttc aaaacctgaa gccaacatct
     8641 gatgaaaacg ctgctcaatg cgttgatgga gcagttcacg gctcgccggg gcgatggcga
     8701 actgatgcac ctgatacggt agagcgtctc ctgacgtttg cgtcagttcc gttaaagttt
     8761 tacccgaaat aaaaaaaact tccagtgccc gggaaagcct ttgtggatca tttggatgaa
     8821 tccttgctgc cgcaaccgga tctacctcct gaagttgacg atgcaatgac tcccaacctt
     8881 gctctgccgc ctgttgctca attctggccc gcacttccgg gtctgccgac ggtagcggcg
     8941 acaacccttc cagcaatgcc ttgaaataca acatcgtacc gcccactaac agtgggatcc
     9001 gccccgccgc ggtgatatcg gccatttccg ccagcgcatc gcggcgaaaa tcagcagccg
     9061 agtaagcctg cgacggatcg cgaatatcca gcaatcggtg cggcgcggcg agtaactctt
     9121 cagcgttcgg cttcgccgtc ccgatatcca tccctttgta aataagggca gaatcaacgc
     9181 ttatcaactc tactggtaaa attttacgca gctcaatggc taacgccgtt ttaccggagg
     9241 ccgtcggccc catcaaaaaa atcgccttag gcaggctcgc cttactgata tcactcatct
     9301 ttcagggctt ttatcgccgg atgtaaatca acagattgta acagaccacc cggcggcgtt
     9361 ttcacaagtt gcggacataa ccgttccacg tccgccagca gggttatggc ctgtgccatt
     9421 gaccactgcg catgttcgct catcagattt cgtgcaatcc actgcgcaat attgccaggt
     9481 tcgaatacgg actgcttcgc caggtagcct atcagttcag gaatcaagat ttgtaaattt
     9541 tgttggcgta agggtaaagg cacggccctg atggtcacat gctgtgcatc tgactggaaa
     9601 tcaataccca attccgctag ggcagactgc gctttttcta atgccgattt ttcttcgcca
     9661 gaaactttta gccgcaacgg aatcagcagc ggctgggcgc aaacgggcgc ttcacccggc
     9721 gtcaattgtg cctgacgcag ccaacgttct gccactggca aggataaaag tgaaatgttg
     9781 ccatcgcgct ccagcaacgc acagtcggaa tggacgatag tcagtacccg accaaaactc
     9841 tgactgttcg ccgcaagtgc aggttcctgc ggttccggcg cttttggttt ttgcatcggc
     9901 gcgggcgttt gcaaaagctg gcgatacact tcaccttgct gtttctggta gcctggctgc
     9961 gcattcggcc agggggcagc cggacgactg cctgaggcgg gcgcaggaga gtagcgcgga
    10021 gctaccggct cacgagctgc cggttctgca aagtgattgc gccccgccgc cacgcggttt
    10081 tccgggatgg cacgcggtgc gggttgtggt tcatcgtcca gcggtagcgg cgtttccagt
    10141 tgctgttgta gcacgctcag cacgccctga tagataaaat catgcaccag acgcgactga
    10201 tggaaacgca cttcgtgttt ggcggggtgt acgttgacgt ccacctgatg cgggtcgatc
    10261 tccagataca acacaaatgc cggttgctga tcggccccca gtttgtcttc gcaggcctgg
    10321 cggatcgcat gattgatcag gcgatcgcgc atcatgcgac cattcacgta gcaatactga
    10381 atttccgcca gtgcgggcgt agtgtgattt ggatcggcca cccagccgcg cagggtgaga
    10441 tcgccgtgtt gccattcaat cgccagcgct tgttcgagaa aagcggtgcc gcaaatcgcg
    10501 cctaagcgcc gttctttttg cccgccttcc ggcactgcgc ggtactgacg cacaatttta
    10561 ccgttatgcg acaggttgat cgtgacgtcg aaacgcgcca gcgcaatgcg gcggatgatt
    10621 tcatcaatat ggctaaattc ggttttctcg gtgcgcagga atttgcgccg cgccggggtg
    10681 ttgtagaaca gatccagcac ctccagcgtc gttcccaccg gatgcgccgc cggtttaacc
    10741 gtcacgtcca tgtcgcgtcc ttcggcatag gcctgccagg cttcctgctg ttctgcggtg
    10801 cgtgaagtga gcgtcaggcg ggaaaccgaa ctgatactcg ccagcgcctc accgcgaaag
    10861 cccaggctga taatggcttc gagatcgtcc agagaggcga ttttactggt ggcatgacga
    10921 gccagcgcca gcgccagctc atcttttttg ataccgcagc cgttatcacg aatgcggata
    10981 agtttcgccc caccgcgttc gatatcaata tcgatacgcg tcgcacctgc atcgaggctg
    11041 ttttccacta gttctttgac taccgacgca ggtcgctcga ccacctcacc tgcggcaatc
    11101 tggttcgcca gttgtggcgg taagacctga attggcatca atcgtcctta gtttggcagc
    11161 gtgcgatctg gcgtcgtcac cgtactggca gtttgtgccg ttgcaccctg cggcgcagat
    11221 tgcatcggat gcgcaaggaa gtaattgcgc aggcctttgt aaatggcttc tgccagctgt
    11281 tgttggtaat cgtcgctcgc cagcaaacgt tcttcgctgt tgttgctgat aaaaccggtt
    11341 tcgaccagta ctgatgggat atccggcgag cgcagaacgc caaggctggc gtgttctggt
    11401 cgacgtttat gtatttcgcc aatgcgttgc aactgactga tcatactggt cgctacatca
    11461 taccctaccc gctgggaatg accgaactgt aaatccagca ccgcctggct taaatagggg
    11521 tcagactgac tgttcgccag cacatcaccc gccccaccca gcagctccga ctgtttctcg
    11581 tgctgctcca gccagctggc catttcactg ttggcgcgac ggttagagag caccaatacg
    11641 gaagcgccag tcgcactgcg gttcggtgcg gcatcagcgt gaatcgacac gaggaaattg
    11701 gcgttttgct tatgtgccac atcactgcgc cccatcaccg agataaagta atccccgtca
    11761 cgggttaaaa cgcctttaaa catcggatcg tcattgagca aagtacgcaa tttacgcgcg
    11821 atggcgatgg tgacattttt ctcccgcgta ccaccggggc cgatagcgcc agggtcctga
    11881 ccgccgtgtc cggcatcaat agcgatgata attttatcgc cagtgttagc cgtcgcgcgc
    11941 gctgccggac gcgttaccgt attactgctg ataacacccg tagtgcggtt actttccgtt
    12001 ttaaacggat tgcgcgccgg ttcgctgacg cgcggtgcga caaccgcagg cgtttcaacg
    12061 cgtttcgcaa ccacgggcgg cggaggaggc ggtggcggca catcggcgtt aatcgtaaag
    12121 acgacagtgt aattgctgcc attctgccgc ttcaccgctt cggttttacc gttttcggta
    12181 agatcgacca ccagccgtag cgtttgtgca tctttaggcg ttccagagcg aatcgccttc
    12241 accagattat tgccgctgaa caacaacggc agtccctgaa tcacgcccgt ttgtttgata
    12301 tcgagcgcca cggtgcgttt gctttgatgg ctaaacgcat aatcaggatc gccaataaaa
    12361 ctcaacgtta tccgtgcctg ttgattaccg ttagaaacct gaatatcaga gagcgtcgcg
    12421 gcacccaccg gcgtgcacag cagcagcagc gtcgctacca accaatttct gatgcgatac
    12481 atcatcccgc cacctttcaa aggttaaccg gctaaacgcg ccagcaacaa ttcacccgca
    12541 gaggaaaccg cactcacgcg cgcctcacgg ccttgtgcct gataatcaat gtgtatttcg
    12601 acatccgggt caggaagaac acctgtacct tgttgtggcc actccacaag gcagatggca
    12661 tcgttggcaa aataatcgcg gatccccata aactccagct cctcgggatc ggcaaggcgg
    12721 tacaaatcaa agtgatagac cattaagttg tcgagcgtat agggttcgac cagcgtataa
    12781 gtggggcttt tgacattacc ctgatgaccc agagcctgta aaaagccccg gctaaaggtg
    12841 gttttacctg cgcctaaatc gccatacaga tagattacgg ttgctccatc gcaggctttc
    12901 gctacccgct cgcccaggtc taatgttgcc tgctcatcag ggagcggaat tactcgattc
    12961 atcatggttt ttatcagtca cttccgggtt aacaatacgc tgtagcgtgg aaaagagatc
    13021 ggttgccagc atcccgcgcg ttccaaaacg cgccgccagt acgtcagctg ccgcaccgtg
    13081 cgcgacacag cctgcacagg ctgcatcata cggcgacagt ttttgcccaa gcaatgcgcc
    13141 aataatacca gagagcacat cgcccatgcc gccgctcgcc atgcctgcat ttccggcatc
    13201 aataatgcct aaagcgtcag gatgggcggc gaccacggtt ccggcacctt tcagcaccgc
    13261 tacgccgcca taacgttgta ccagacgttt ggcgcaatgt aagcggtcac tttcaatttc
    13321 agcgacggaa cagcctaaca accgtgcggc ctcgctagga tgcggcgtga tcacgcgatt
    13381 gtgacgctta tcgggattga ttgccagcag gttcaatgca tcggcatccc acaacatcgg
    13441 tttgcgaaaa ttctcaactt tttgcagggc ttttttcccc cactcttgct ggcccagacc
    13501 gggaccaatc accaccacat cggcccattc caggctttcg gtaagagagt ccatagtcag
    13561 ttcatgcacc atcaattctg gtcgtgcagt cagcagcggc gcaatgttct cactgcgggt
    13621 cagtactcgg actaaaccag caccagcacg tagcgccgct tcccccgtca tacgaatagc
    13681 ccccgccgtg ccgtgatcgc caccgataat caccagccgc ccgtgatcgc ctttatgcga
    13741 agtcgggcga cgcggtttca gccagtgaga aagttgttct gccgaaaacc gctgaatttt
    13801 cgtctcctga cctgccagcc agctatccag ccccagtgag tcaaaatgca gttgtcctgt
    13861 aacatcccgc gcttttccag tgagcaagcc tagtttcagc gcaataaaag tgatggtgtg
    13921 atcggcgttg atcactgcgc ctggcgtagc gccggtttca gccagcaggc cggaagggat
    13981 atcaaccgcc acaatcggcg caggatggga attagcgtgg tcgattaact ggctaatgga
    14041 ttcgcggggc gcttgctgca agccggtacc gagcagcgca tcaacaatca gatctaccga
    14101 ttcgggccag acaatattcg aagcatggat ctcgccaccc gcgtttaacc atgcttcgcg
    14161 tgccagcgcg gcctcttccg gcaacggttt gtcgctctcc tgggccaaca acgtgacctc
    14221 aatgccgacc gctttggcca gtcgcgcgac cacgtagcca tcgccgccgt tattaccatg
    14281 accgcacagc accagccagt ggcgggcgtc aggatacgcc gaacgacaca cctggaatgc
    14341 ggcctcgcca gcgcgaagca tcagctcata gagtgtgagc cccagcacat ctgccgcctc
    14401 gcgttctccg cggcggatat cgtcggcgta ccagacggtg tgtggtatac ttacggggtt
    14461 tttcttcatt gtatggtccg tcatgtcaga gcccctcgat ctcaatcagt tagcgcaaaa
    14521 aattaaacag tgggggctgg aactgggctt tcagcaggta ggtattaccg ataccgatct
    14581 cagcgagtcc gagcccaaac tgcaagcatg gctggacaaa caataccacg gcgaaatgga
    14641 ctggatggca cgtcacggta tgctgcgcgc tcgcccccat gagttattac ccggtacgct
    14701 gcgtgtaatc agcgtgcgga tgaattacct tcctgctaac gccgcatttg ccagcacgct
    14761 gaaaaacccc aaactcggct atgttagccg ttatgcgctg ggccgtgact atcacaaact
    14821 tctgcgcaac cgactcaaaa agctgggcga gatgattcag caacattgtg tttcgctgaa
    14881 ttttagaccg tttgtcgatt ctgcgcctat tctcgagcgc ccgttagctg aaaaagctgg
    14941 gctcggctgg acaggtaagc actcacttat cctcaatcgc gaggccggtt cgttcttctt
    15001 tttaggcgaa ttgctggtcg atattccgct gcccgtggat caaccagtcg aggaaggatg
    15061 cggcaaatgc gtggcctgta tgacgatttg cccgaccggt gccatcgtcg agccatatac
    15121 cgtcgatgct cgccgctgta tctcttatct caccatcgaa cttgaagggg cgatcccgga
    15181 agagttgcga ccgttaatgg gaaaccgtat ttacggttgc gatgactgcc agcttatctg
    15241 cccgtggaat cgctattcgc aactcactac agaagacgat ttcagcccgc gtaagccgct
    15301 acacgcaccg gaactcattg agttatttgc ctggagcgaa gagaagtttt taaaagtcac
    15361 ggaaggatcg gcgattcgcc gtataggtca cctgcgctgg ctgcgtaata tcgccgtggc
    15421 attaggcaat gccccttggg atgaaacgat tttgacagcg ctggaaagtc gtaaaggtga
    15481 gcacccactt ctcgatgagc acatagcgtg ggcgattgcg cagcaaatag agagacgaaa
    15541 tgcgtgcata gtcgaagtgc aattgccgaa aaaacagcgt ctggttcggg tgattgaaaa
    15601 agggttaccg cgtgacgcct gattcatcca cagcttgtga ataaaaataa aaacgcattg
    15661 caattcaaga cgaataaaat cgtcaagtga tcgactcgac aaaacggaat cttgattttt
    15721 aacttattta tcaataggtt aaaaaatacc attcaccgtg cgaagtttct ttgaaattaa
    15781 gattacagcc tgaggctgtg gataactctg tttacaagag tttgtcagag acaacaaaaa
    15841 ccagcaataa cgcatcgcgt cgctgtggat attttattga gagaaga
//
LOCUS       DAJVQH010000090        15881 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJVQH010000090 DAJVQH010000000
VERSION     DAJVQH010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.090"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..378
                     /gene="tuf"
                     /locus_tag="OQM87_002872"
     CDS             <1..378
                     /gene="tuf"
                     /locus_tag="OQM87_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1508940.1"
                     /translation="RAGENVGILLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILS
                     KDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDD
                     GLRFAIREGGRTVGAGVVAKVLG"
     gene            561..755
                     /gene="bfd"
                     /locus_tag="OQM87_002873"
     CDS             561..755
                     /gene="bfd"
                     /locus_tag="OQM87_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCS1508941.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            828..1304
                     /gene="bfr"
                     /locus_tag="OQM87_002874"
     CDS             828..1304
                     /gene="bfr"
                     /locus_tag="OQM87_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCS1508942.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1301..1768)
                     /locus_tag="OQM87_002875"
     CDS             complement(1301..1768)
                     /locus_tag="OQM87_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCS1508943.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            2147..2458
                     /gene="rpsJ"
                     /locus_tag="OQM87_002876"
     CDS             2147..2458
                     /gene="rpsJ"
                     /locus_tag="OQM87_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCS1508944.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2491..3120
                     /gene="rplC"
                     /locus_tag="OQM87_002877"
     CDS             2491..3120
                     /gene="rplC"
                     /locus_tag="OQM87_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCS1508945.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3131..3736
                     /gene="rplD"
                     /locus_tag="OQM87_002878"
     CDS             3131..3736
                     /gene="rplD"
                     /locus_tag="OQM87_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCS1508946.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3733..4035
                     /gene="rplW"
                     /locus_tag="OQM87_002879"
     CDS             3733..4035
                     /gene="rplW"
                     /locus_tag="OQM87_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCS1508947.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4053..4874
                     /gene="rplB"
                     /locus_tag="OQM87_002880"
     CDS             4053..4874
                     /gene="rplB"
                     /locus_tag="OQM87_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCS1508948.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4891..5169
                     /gene="rpsS"
                     /locus_tag="OQM87_002881"
     CDS             4891..5169
                     /gene="rpsS"
                     /locus_tag="OQM87_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCS1508949.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5184..5516
                     /gene="rplV"
                     /locus_tag="OQM87_002882"
     CDS             5184..5516
                     /gene="rplV"
                     /locus_tag="OQM87_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCS1508950.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5534..6235
                     /gene="rpsC"
                     /locus_tag="OQM87_002883"
     CDS             5534..6235
                     /gene="rpsC"
                     /locus_tag="OQM87_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCS1508951.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6248..6658
                     /gene="rplP"
                     /locus_tag="OQM87_002884"
     CDS             6248..6658
                     /gene="rplP"
                     /locus_tag="OQM87_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCS1508952.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6658..6849
                     /gene="rpmC"
                     /locus_tag="OQM87_002885"
     CDS             6658..6849
                     /gene="rpmC"
                     /locus_tag="OQM87_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCS1508953.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6849..7103
                     /gene="rpsQ"
                     /locus_tag="OQM87_002886"
     CDS             6849..7103
                     /gene="rpsQ"
                     /locus_tag="OQM87_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCS1508954.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7268..7639
                     /gene="rplN"
                     /locus_tag="OQM87_002887"
     CDS             7268..7639
                     /gene="rplN"
                     /locus_tag="OQM87_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCS1508955.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7650..7964
                     /gene="rplX"
                     /locus_tag="OQM87_002888"
     CDS             7650..7964
                     /gene="rplX"
                     /locus_tag="OQM87_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCS1508956.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7979..8518
                     /gene="rplE"
                     /locus_tag="OQM87_002889"
     CDS             7979..8518
                     /gene="rplE"
                     /locus_tag="OQM87_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCS1508957.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8533..8838
                     /gene="rpsN"
                     /locus_tag="OQM87_002890"
     CDS             8533..8838
                     /gene="rpsN"
                     /locus_tag="OQM87_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCS1508958.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8872..9264
                     /gene="rpsH"
                     /locus_tag="OQM87_002891"
     CDS             8872..9264
                     /gene="rpsH"
                     /locus_tag="OQM87_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCS1508959.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9277..9810
                     /gene="rplF"
                     /locus_tag="OQM87_002892"
     CDS             9277..9810
                     /gene="rplF"
                     /locus_tag="OQM87_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCS1508960.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9820..10173
                     /gene="rplR"
                     /locus_tag="OQM87_002893"
     CDS             9820..10173
                     /gene="rplR"
                     /locus_tag="OQM87_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCS1508961.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10188..10691
                     /gene="rpsE"
                     /locus_tag="OQM87_002894"
     CDS             10188..10691
                     /gene="rpsE"
                     /locus_tag="OQM87_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCS1508962.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10695..10874
                     /gene="rpmD"
                     /locus_tag="OQM87_002895"
     CDS             10695..10874
                     /gene="rpmD"
                     /locus_tag="OQM87_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCS1508963.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10878..11312
                     /gene="rplO"
                     /locus_tag="OQM87_002896"
     CDS             10878..11312
                     /gene="rplO"
                     /locus_tag="OQM87_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCS1508964.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11320..12651
                     /gene="secY"
                     /locus_tag="OQM87_002897"
     CDS             11320..12651
                     /gene="secY"
                     /locus_tag="OQM87_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCS1508965.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12683..12799
                     /gene="rpmJ"
                     /locus_tag="OQM87_002898"
     CDS             12683..12799
                     /gene="rpmJ"
                     /locus_tag="OQM87_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCS1508966.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12946..13302
                     /gene="rpsM"
                     /locus_tag="OQM87_002899"
     CDS             12946..13302
                     /gene="rpsM"
                     /locus_tag="OQM87_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCS1508967.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13319..13708
                     /gene="rpsK"
                     /locus_tag="OQM87_002900"
     CDS             13319..13708
                     /gene="rpsK"
                     /locus_tag="OQM87_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCS1508968.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13742..14362
                     /gene="rpsD"
                     /locus_tag="OQM87_002901"
     CDS             13742..14362
                     /gene="rpsD"
                     /locus_tag="OQM87_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCS1508969.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14388..15377
                     /gene="rpoA"
                     /locus_tag="OQM87_002902"
     CDS             14388..15377
                     /gene="rpoA"
                     /locus_tag="OQM87_002902"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCS1508970.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15418..15801
                     /gene="rplQ"
                     /locus_tag="OQM87_002903"
     CDS             15418..15801
                     /gene="rplQ"
                     /locus_tag="OQM87_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCS1508971.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4087 a         3739 c         4234 g         3821 t
ORIGIN      
        1 cgtgctggtg agaacgtagg tattctgctg cgtggtatca aacgtgaaga aatcgaacgt
       61 ggtcaggtac tggctaagcc gggcaccatc aagccgcaca ccaagttcga atctgaagtg
      121 tacattctgt ccaaagatga aggcggccgt catactccgt tcttcaaagg ctaccgtccg
      181 cagttctact tccgtactac tgacgtgact ggtaccatcg aactgccgga aggcgtagag
      241 atggtaatgc cgggcgacaa catcaaaatg gttgttaccc taatccaccc gatcgcgatg
      301 gacgacggtc tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgtt
      361 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
      421 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      481 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      541 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      601 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      661 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      721 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      781 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      841 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      901 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      961 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
     1021 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
     1081 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
     1141 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
     1201 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
     1261 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
     1321 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1381 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1441 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ccaggcgcca agggcggcga
     1501 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1561 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1621 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1681 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1741 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1801 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1861 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1921 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1981 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     2041 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     2101 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     2161 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     2221 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     2281 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     2341 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2401 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2461 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2521 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2581 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2641 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2701 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2761 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2821 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2881 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2941 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     3001 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     3061 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     3121 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     3181 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     3241 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     3301 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3361 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3421 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3481 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3541 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3601 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3661 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3721 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3781 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3841 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3901 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3961 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     4021 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     4081 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     4141 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     4201 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     4261 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     4321 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4381 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4441 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4501 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4561 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4621 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4681 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac tgcgatgaac ccggtagacc
     4741 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4801 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4861 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4921 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4981 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     5041 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     5101 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     5161 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     5221 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     5281 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     5341 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5401 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5461 cagatcgtat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5521 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5581 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5641 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5701 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5761 tatcggtaag aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5821 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5881 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5941 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     6001 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     6061 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     6121 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     6181 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     6241 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     6301 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6361 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6421 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6481 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6541 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6601 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6661 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6721 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6781 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6841 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6901 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6961 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     7021 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     7081 ttgtagagaa agcggttctg taatacagta cactctctcg atatgaataa acggctcaga
     7141 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     7201 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     7261 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     7321 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7381 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7441 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7501 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7561 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7621 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7681 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7741 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7801 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7861 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7921 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7981 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     8041 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     8101 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     8161 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     8221 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     8281 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     8341 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8401 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8461 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8521 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8581 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8641 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8701 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8761 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8821 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8881 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8941 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     9001 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     9061 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     9121 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     9181 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     9241 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     9301 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9361 aaaaacggcg agctgacccg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9421 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9481 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9541 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9601 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9661 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9721 cgcgcctacc gtcgtcctga gccttacaaa ggcaagggtg ttcgttacgc cgacgaagtc
     9781 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9841 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9901 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9961 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
    10021 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
    10081 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
    10141 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
    10201 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
    10261 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
    10321 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10381 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10441 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10501 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10561 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10621 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10681 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10741 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10801 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10861 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10921 cgggtaaacg cctggggcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10981 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    11041 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    11101 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    11161 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    11221 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    11281 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    11341 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11401 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11461 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11521 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11581 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11641 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11701 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11761 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11821 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11881 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11941 gagcaagcgc gtcaaggtga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    12001 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    12061 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    12121 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    12181 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    12241 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    12301 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12361 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12421 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12481 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12541 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12601 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12661 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12721 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12781 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12841 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12901 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12961 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    13021 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    13081 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    13141 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    13201 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    13261 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    13321 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13381 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13441 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13501 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13561 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13621 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13681 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13741 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13801 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13861 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13921 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13981 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    14041 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    14101 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    14161 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    14221 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    14281 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    14341 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14401 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14461 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14521 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14581 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14641 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14701 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14761 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14821 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14881 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14941 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    15001 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    15061 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    15121 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    15181 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    15241 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    15301 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15361 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15421 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15481 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15541 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15601 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15661 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15721 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15781 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15841 ttatacccgt agtatcccca cttatctaca atagctgtac t
//
LOCUS       DAJVQH010000091        15876 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJVQH010000091 DAJVQH010000000
VERSION     DAJVQH010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.091"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..358)
                     /locus_tag="OQM87_002904"
     CDS             complement(<1..358)
                     /locus_tag="OQM87_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCS1508972.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQT"
     gene            complement(355..3039)
                     /locus_tag="OQM87_002905"
     CDS             complement(355..3039)
                     /locus_tag="OQM87_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCS1508973.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNTDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            complement(3131..3631)
                     /locus_tag="OQM87_002906"
     CDS             complement(3131..3631)
                     /locus_tag="OQM87_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1508974.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            complement(3661..4377)
                     /locus_tag="OQM87_002907"
     CDS             complement(3661..4377)
                     /locus_tag="OQM87_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1508975.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            4673..5449
                     /gene="exuR"
                     /locus_tag="OQM87_002908"
     CDS             4673..5449
                     /gene="exuR"
                     /locus_tag="OQM87_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCS1508976.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            5794..6456
                     /locus_tag="OQM87_002909"
     CDS             5794..6456
                     /locus_tag="OQM87_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1508977.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            6460..6843
                     /gene="mzrA"
                     /locus_tag="OQM87_002910"
     CDS             6460..6843
                     /gene="mzrA"
                     /locus_tag="OQM87_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCS1508978.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            6990..7344
                     /locus_tag="OQM87_002911"
                     /pseudo
     CDS             6990..7344
                     /locus_tag="OQM87_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            7382..7687
                     /locus_tag="OQM87_002912"
     CDS             7382..7687
                     /locus_tag="OQM87_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1508979.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            7690..8094
                     /locus_tag="OQM87_002913"
     CDS             7690..8094
                     /locus_tag="OQM87_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS1508980.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            8084..8383
                     /locus_tag="OQM87_002914"
     CDS             8084..8383
                     /locus_tag="OQM87_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCS1508981.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            8569..8961
                     /locus_tag="OQM87_002915"
     CDS             8569..8961
                     /locus_tag="OQM87_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1508982.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTTLFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            9031..10017
                     /gene="yqjG"
                     /locus_tag="OQM87_002916"
     CDS             9031..10017
                     /gene="yqjG"
                     /locus_tag="OQM87_002916"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCS1508983.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            10310..10678
                     /locus_tag="OQM87_002917"
     CDS             10310..10678
                     /locus_tag="OQM87_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1508984.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(10885..11781)
                     /gene="yhaJ"
                     /locus_tag="OQM87_002918"
     CDS             complement(10885..11781)
                     /gene="yhaJ"
                     /locus_tag="OQM87_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCS1508985.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            11886..12587
                     /locus_tag="OQM87_002919"
     CDS             11886..12587
                     /locus_tag="OQM87_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCS1508986.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            12610..12774
                     /locus_tag="OQM87_002920"
     CDS             12610..12774
                     /locus_tag="OQM87_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1508987.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(12986..14278)
                     /locus_tag="OQM87_002921"
     CDS             complement(12986..14278)
                     /locus_tag="OQM87_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCS1508988.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(14306..15637)
                     /locus_tag="OQM87_002922"
     CDS             complement(14306..15637)
                     /locus_tag="OQM87_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCS1508989.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTCWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
BASE COUNT         4133 a         4039 c         3677 g         4027 t
ORIGIN      
        1 tcgtctgaag ccctttgccc cccttgcatc ctagcggcgc ttcatcgcta cttacaatgg
       61 ctgttttaaa tcctttgagt tttaccggcc aacgcatccc actgcgttgt tcagttaaat
      121 agatttgtac tgtcgatata tttccattag ctgtacaggt ttgttggagg tcaggatacg
      181 aacattcata ccacatatat ccattatttc ctgccgacgc ttcatccttc cagaagtaat
      241 aatccgctct accagatgcc aaatcatacg taatttcact ggtcacgtca ggagctgcct
      301 gaacgccagt gagcgtgcca aacaagccca atatcgccgc aatcaatcga tttctcatgg
      361 tgcaacctcc ctttgcgccg tctgaacgtt tccacgtaat ttaggctgtt tcaactgtgc
      421 ctgaacctga atcgactccg gtaattgtga ataagtcacc acactacaag taacatctcc
      481 gctgtaacga atgctggcgc gtttctttac aacactcagt ggacacttaa ggaattgctg
      541 cccggaaagc agatagagct ctttttcgct gccgttaatt tcaagtgtaa atccgccatc
      601 ttcagctacc agtaacatgg cgctgttcag ccccacaatc gggaattttt tcaccgctgc
      661 cggcagtaat aaacgaccga tatagttgta gttaaagctg gcagacactt cgcgatagcg
      721 cagcttgcca ggcaacaaca ttaattcacg attccctgca ccgttaatga tgtttgttgt
      781 cccctgctga ttgccctggc tactgtcgtt aacctcaaca aaaccagact gaaatccagg
      841 ataagggaac aacacactgt cactttgacc aagctgcaaa gttcggctgc ctgccacccg
      901 cgcttcgagt tgcagatcct gactctcttc atcctgttca gagaagcctt tcacatccac
      961 cagtaccgca cccagcaaat cgtctgtacc actggctccc aaattaacgc catagcgact
     1021 gacagccagc gtagagctat aagtgcccgt aaaggccgaa agatgatcat gattcttacg
     1081 gtcataacta tcagatacgg tagcgtagac atttccgtat tgcccatccc aacgcccgtt
     1141 aaccgcgctg tttatggtat cggtgttcag gcctccgacg gttacgccaa gttctttatg
     1201 gctgaatgaa tcgttataaa aggtatgcga taactgccat gacgtttgat cgccatcctg
     1261 ttcgctataa cgataatccg tagacacatt ggttgaatgg ctgttgttat tgctatccat
     1321 cgttggcgtg tcagataacg aaaacgataa gtacaagccg ttgtcgtcgt tatcgccacc
     1381 gttgtcatta cgatggaaaa caccgactga cgtactcaaa atccagtcac gcatcacaat
     1441 agcgcgggaa gcgctaagtt gtacagtttc agagatactt tgcgctgttg tctggcgaag
     1501 atagttattt tcaaagctct gctgtttatc ccaacgcaaa ctatcatcag tgttattttg
     1561 tgtgcaggta taacccagca tcatattcca gccgacaaaa ttcagtgaaa ctgtcgcgga
     1621 aatactttca taacagctta aggcgttata gctctctctg ctgtcggttg tacaagcatc
     1681 gccgtcagag ttggtccgat aaaagcccaa cgtcggccat cccggattac tccagttggc
     1741 ctgttgcata tcacctttgc cgccgtcatc gttacggaac acgctggcgc tgattgcaag
     1801 attccccacc ccgcctaaat ctgccgtcca gttattacct aactcgaaag cactcacatc
     1861 atcggcattc gccagccctg cgtagagctc atattgcgga tgtaatggca ggcgtactcc
     1921 tagctgataa gctgaacttt catcatcaga aacctgtgat gtagttttac ctgcctgtaa
     1981 gaaccattgc gcatttccgt cagtcagacc gccggttttg gtaaacggca caagctcggt
     2041 gcgggtgagt tggttatttt cgtagacttt taatgccacg ctgtagctgc ctggcggaaa
     2101 ggaactggta tcaataaatt gcgaaccact attgagataa aacgatccca aaagttgctc
     2161 attacgataa gcgtcaacac gagaattgcg cgaaagcaga accattactg gggttccttg
     2221 ctggctttgc gcctggttta aatagctcag tgtcgacccg atacgcatcc catcgattgc
     2281 accgagtggc agaaagttaa aggtaaagtt cccgccttgt gcattaaata gtgtgcggtt
     2341 gtccatgcga cccgcctgca cataataacg acgtaaaaaa tcataacgat aatagagatc
     2401 gctgacgtca gtattactgt cactgacatc atctgcatca taaccgttga aattccagtg
     2461 tgcaccaata tagctatttt ctgttattcc cagcgcaccg tttccctgaa tagacaacga
     2521 ttggtaatca tcctgtgcca gaacattgat gtcttgctga tgtataaaag cgttaacagt
     2581 atttttgtct ggatttaaat ataatgactt cgcatcgaaa gctgaattcc attgcggatt
     2641 aataaacaac gttgccgaac tttcttcatc attatagatt atttccagcg tatcggtatt
     2701 cagaaaacca caccctgagt cagtacgtac ataaccgcaa gccaattcac cgtggcgagc
     2761 tagcggctgc gataattttt ctttcactat atgcgcgatt ttctgatcgt ctacatccag
     2821 ttccagcttg ttatatagac ttgctggatc aagaaatgtt acggtatcaa gattaaccat
     2881 tgcataatat ttaccgagac ttttccctga aaaataaacc tcgatatact cctgctgtcc
     2941 ctgagcaata acctcaaacc ctgcgggaat tttggttaat gtcgcctctg ccgggctgag
     3001 actcgccagg atcaaaagag ccagtaattt tttatccatg gcaatcctga aatccataac
     3061 aaatactaaa acagttaaaa taagggaggg ctacccctcc ctttgttacg tgttaattct
     3121 gttatccgga ttaaatatcc tgagacagat aaatgcttac tacaccacgg tagatgcctg
     3181 tttccagaac acctttggtg gtttgagcaa acatcaggtt tttagccaga gaaccgttag
     3241 ttaacgcatc ggaagcaaag attttggttg cggtaaaagt ggttgccgca tcagctttaa
     3301 tttcttcacc accccaggtt actgtcagcg gaacaatctt gctagcatcc aggctttgaa
     3361 ccagttgcgc tggggacact aactgcattt ttacatcttt ggtaacgtcg ttagaccaga
     3421 ttttggtcat caactggtaa gattgcagac cctgccccgg cagatattgc atttctactg
     3481 ctttcggcaa cgcggtgtta tcggtctgtg tcatatccag agcggcatca acgttggccg
     3541 ttacagtaat atccttttgt actgccaatg cggagcctgc aacagaaaac atcgctgcga
     3601 ccagaagaga ttttgcaaat acctttttca ttactattcc ttcgtttatt acaatgataa
     3661 ttaatggacc gccagttcaa actcgccatt atctttggtg atccagttat tatatttgac
     3721 aacgattttg tcgtagccag atatgctttc aagatttctt tcctgaccag ggaatatgtt
     3781 tttatttacg gtctgtatct tacagttttc tttgctatta tttttacaat aagcgatcgt
     3841 taaaagcttt aagcgttgat tccctgcatt aattaatttt tgggcagaat ttacttctaa
     3901 cttagctata ggttgctgag gtggaacact aacaacgatt ccccaaatta agttaactgt
     3961 taactgcata gagagatctt ttttatctgt ggcaacgtca tcaatactgg gaaccgcttc
     4021 aaatttaacg cgataatttt tctctttttg ctctggttcc atcgcaacaa aacgtacggt
     4081 tttgctacta ccggctggta aagcgaattt aggtggcata acaaccagat gatttgcatc
     4141 accggattta atctcaactt cattttcctg aggcgtcgat ggattatcga tacggaatac
     4201 tgtcgccttg atgtactgaa cttcattact tttagatatt acgcgaacgt tgccttcacc
     4261 ctgactatta atggaaacag gcataggata aacagtcata tttgactgtg ccaaaaaagg
     4321 agcgaacacg aataaaagcg gcgtaagccg taataagaga ttaattttcg ccaccatata
     4381 attttgatct tacaatatcg tttcaaaaaa gttggtgtat tagagatgta gatttattgt
     4441 aagtcaatat aaaaaagcat ttttagttaa cattaaattt aaaggctggg aattaactta
     4501 ctgaaattta gtagctttca caatgacgat acttattttt taggaattta taaatattca
     4561 caatattaat ttaatacatt aattcaatca tcaaatttat atatgaccaa tggcataccc
     4621 atgaataaag tggtatgata acttcgtccg cattccactc cgggagacgc atatggaaat
     4681 cactgaacca cgccgtttgt atcaacaact tgctgctgac ctgaaagagc gcattgaaca
     4741 gggcgtctat ctggtgggtg ataaactgcc tgccgaacgc tttattgccg atgaaaagaa
     4801 cgtcagccga acggtagttc gtgaagccat catcatgctg gaagttgaag gctatgtgga
     4861 ggtgcgtaaa ggttcaggta ttcatgtggt ttccaaccag ccgcgtcatc agcaggcggc
     4921 tgacaataat atggaattcg ccaattacgg tccgtttgag ttgcttcagg ctcgccagct
     4981 catcgaaagt aatattgccg agttcgcggc aactcaggta acgaaacaag acatcatgaa
     5041 actgatggcc attcaggaac aggcgcgcgg cgaacaatgc ttccgtgatt ccgagtggga
     5101 tttacagttc cacattcagg tagccctggc gacgcagaac tccgccctgg cggccatcgt
     5161 tgaaaaaatg tggacccagc gtagccataa cccgtactgg aaaaaactac acgaacacat
     5221 tgattcccgt accgttgata actggtgtga tgaccacgat caaattctca aggcgttaat
     5281 tcgcaaagat cctcatgctg ctaagctggc aatgtggcag catctggaaa acaccaaaat
     5341 catgttattt aacgaaacca gcgacgattt cgagttcaat gccgaccgct atctgttcgc
     5401 cgaaaacccg gtagtgcatc tcgatacggc caccagcggc agcaaatgaa attcctgtcc
     5461 gacaggcgct gcaatgcgcc tgtttgtctc gtcggtaagc gaaaagtata aagtgtcagc
     5521 ctgtgtaaat cctctcgcca ccctcccctg caatcagcaa aatcagactc cacggacatg
     5581 taattttgat aacgaacaac gttgaccttt gttacaatta gattcaattt gaatttatgc
     5641 ttttgaatgc tttcttatct cacgttttaa cagggaatag ttcaggctgt gttgatgtat
     5701 caaacccgca gaacatacca aaacagcaat aacattgcgg tagtgcatct tttaaaacca
     5761 gcgtggcgtt aaccgattca ccaggaataa tgaatggaac ttttgaccca attgctgcaa
     5821 gccctgtggg cgcaggattt tgaaaccctg gccaatccat cgatgattgg catgttgtat
     5881 tttgtcttgt ttgtaatttt gttccttgaa aatggcttgc ttccggcggc ctttttaccg
     5941 ggcgacagtt tactggtatt ggtcggcgtg ttgattgcga aaggcgcgat gggttacccg
     6001 caaacgattc tgctgctgac cgttgccgcc agcctcggtt gctgggtcag ctatattcag
     6061 gggcgatggc tgggcaatac ccgcaccgta caaaactggc tatctcattt acccgcgcat
     6121 tatcatcaac gcgcacacca tctttttcat aaacacggtt tatcggcgct gttaattggt
     6181 cgctttattg catttgtcag aacactgctg ccgacgattg ccgggttatc agggctgaat
     6241 aacgcgcgct ttcagttctt caactggatg agcggtctgc tgtgggtatt gatcctgaca
     6301 actctgggtt acatgctcgg caaaacgccg gtatttttaa agtacgagga ccagctgatg
     6361 tcatgcctga tgctgctccc ggtggtgctg ctggtgtttg gcctggcagg ttctctggtc
     6421 gtgttatgga aaaagaaata tggaaatcgg gggtaaggga tgcaaatacc tcgcatgtcg
     6481 cttcgccagc tagcctggtc cggcactgtt ttacttctgg tcggcacgct gttgctggcc
     6541 tggtccgcgg ttcgccagca agagtctacg ctggcgattc gtgccgttca tcaaggcaca
     6601 acgatgccag acggtttttc gatctggcat caccttgacg ctcatggcat tcctttcaaa
     6661 agtatcaccc ccaaaaacga cactctgtta attacatttg actccagcga ccagagcgcc
     6721 gccgcaaaag cggtcctcga cagaacattg ccccatggct acatcattgc gcagcaggac
     6781 aataacagtc aggctatgca gtggctgacc cggttacggg ataactctca tcgcttcgga
     6841 taacttcctg ttatccgaaa catatcactc actttggtga tttcaccgta actgtctatg
     6901 attaatgagg cggtggttaa cactaccgct tcgttgtctg gacaatcgtt cctttgtaat
     6961 aggtccgaat cacaatggaa ggttcaagaa tgaaataccg catcgcttta gctgtttctc
     7021 tctttgctct tagtgccggt agttatgcca ctaccctgtg tcaggaaaag gagcaaaata
     7081 tccttaagga gatcagctat gccgaaaaac accaaaacca gaatcgtatt gacggtctga
     7141 ataaagccct gagtgaagtc cgggccaact gttcagatag ccagctgcgt gccgatcatc
     7201 acaagaaaat cgcaaagcag aaagatgagg tggcggaacg ccagcaagat ttagccgagg
     7261 cgaagcaaaa aggcgatgcc gatagaacgg aaactggcag aagcgcagga agagctgaaa
     7321 aagctggaag cgcgcgacta ctaactcaca atagtcacta cttactcacc tggagaaaac
     7381 tatgtcgaaa gaacacacta cggaacatct gcgtgctgag ttgaaatccc tttccgatac
     7441 gctggaagag gtgcttagct catctggcga gaagtcgaaa gaagagttga gtaagattcg
     7501 tagcaaagcg gagcaggcac tgaaacagag ccgttatcgc ctgggtgaaa ccggtgatgc
     7561 catcgccaaa caaacccgtg tcgcggcggc gcgtgctgat gagtatgtgc gtgaaaatcc
     7621 gtggaccggc gtgggaattg gcgctgcaat cggtgtagtg ctcggcgttc tgctgtcgcg
     7681 tcgttaatta tggcggacac tcatcacgcg caagggcccg gtaaaagcgt tctgggcatc
     7741 gggcagcgaa ttgtttccat catggttgaa atggtggaaa cacgtctgcg gctggcggtg
     7801 gtggagctgg aagaggaaaa agctaatctc tttcaacttt tactgatgct gggcctgacg
     7861 atgcttttcg ctgcatttgg tcttatgagc ctgatggtgc taattatttg ggcggttgat
     7921 ccgcaatatc gcctgaatgc gatgattgcc accaccgtgg tgttgctgct actggcactg
     7981 attggcggta tctggacgtt acgtaaatcg cgtaaatcca ccttgctgcg ccatacacgc
     8041 catgagctgg caaacgatcg gcaactgctc gaggaggagt cccgtgagca gtaaagtcga
     8101 acgtgaacga cgtaaggcgc aactgcttag ccagatccag caacaacggc tggatctttc
     8161 cgccagtcgt cgtgaatggc tggaggcaac aggcgcttac gatcgtcgct ggaatatgct
     8221 gctaagtctg cgctcctggg cgctggttgg cagtagcgtg atggcgatct ggacgattcg
     8281 ccatcctaat atgctggtcc gctgggccag acgcggtttt ggcgtatgga gcgcctggcg
     8341 tctggttaaa acgaccctca agcagcaaca gcttcgcggt taacttccct ctggccggag
     8401 caattccggc cttatccctc aaattttttg aatatttttg acagttttcc ttgctaacaa
     8461 tcatcattca ccacgtttat gattctctcc atcgacagca acgacgctaa taccgcgccc
     8521 ttgcacaaaa aaacaatcag cagcctgagt ggcgcagtgg agagtatgat gaaaaaatta
     8581 gaagatgttg gtgtactggt agcgcacatt ttaatgccga ttctgtttat taccgcaggc
     8641 tggggaaaaa tcaccgctta ctctggtacc caacaatata tggaagcaat gggtgtcccg
     8701 ggttttatgc tgccgctggt gattctgctt gagttcggtg gtggtctggc aatcctattc
     8761 ggtttcctga ctcgcaccac aaccctgttt actgcgggct ttacgctgct gacggcattt
     8821 ttatttcaca gcaactttgc tgaaggcgtc aactcgctga tgttcatgaa aaacctgaca
     8881 atttctggcg gattcctgct gctggcaatt accggtccgg gcgcgtatag catcgaccgc
     8941 ctgctgaata aaaagtggta agcacgcgct atacttaacg ataaaaaacg aggaggaagc
     9001 tcctcgtttt tgctattgga ggagagaaaa atgggtcaac tgattgacgg cgtctggcat
     9061 gacacctggt acgataccaa atctaccggc ggtaaatttc aacgttcagc ttccgcattt
     9121 cgtaactggc tcactgccga tggcgcacct ggccccactg gcacaggcgg ttttatcgca
     9181 gagaaagatc gttatcatct ctatgtttca ctcgcctgcc cgtgggcgca ccgcacgctg
     9241 atcatgcgca aactcaaagg actggaaccg tttatttccg tttccgtagt gaacccgctg
     9301 atgctggaaa acggctggac ctttgatgac agttttccgg gagcaaccgg agatacgctc
     9361 tatcaacatg aatttctgta tcagctttat ctccacgccg atccacacta cagcggacga
     9421 gttactgttc ccgtgctgtg ggacaaaaag aaccacacca tcgtcagcaa cgaatcagca
     9481 gaaatcatcc gcatgttcaa taccgcgttt gatgcgctgg gcgcaaaagc gggtgattac
     9541 tacccaccag ccctgcaaac gaaaattgac gaacttaacg gctggattta tgacaccgtt
     9601 aacaacggcg tgtataaagc tggttttgcc accagccagc aagcctacga cgaggcggtg
     9661 gcgaaagtgt ttgaatcgct ggcgcgactg gagcagattt taggccagca tcgttacctg
     9721 accggcaacc agctaaccga agccgatatt cgcctgtgga ctacgctggt gcgttttgat
     9781 ccagtgtatg tgacccactt caagtgtgat aagcaccgca tcagcgatta cctgaatctg
     9841 tatggcttcc tgcgcgatat ctaccagatg ccggggatcg ccgaaactgt caatttcgat
     9901 cacatccgta atcattactt ccgcagccat aagaccatca accctacggg gattatttca
     9961 attggtccgt ggcaggatct cgatgaaccg catggacgag atgttcgctt cggttaaaaa
    10021 aaggcacctt acgaggtgcc tctgataatt agctaagtgc atgttgttca tgtcggatgc
    10081 ggcgtgaacg ccttatcaaa cctacaaagc gtttatattc aatatgttac aatgaccgtg
    10141 taggccagat aagcgtagcg catctggcga ttttgcgctt gccatcagtc ttagcgcgcc
    10201 ttcacggcac tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt
    10261 cttaatttgc tgctttaaaa acaagtgatt gagcaaaatt gaggcaaaaa tggactggta
    10321 tctgaaagta ctaaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg
    10381 gatgtttatt ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt
    10441 aggctggcaa cgcgctggtg gcgaaggcat cctgacgaca atctacggta ttctggtgtt
    10501 tttaccgtgg tgggcggttc agttccgccg cctgcacgac accgaccgct cggcgtggtg
    10561 ggcactgcta ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca
    10621 ggcgggtacg ccaggcgaaa accgctttgg accagatcca aaactggaac aagaataatg
    10681 attaagcacc ttgtaaaagg tgcttcaaat tgttgacaaa gtgcgctttg tttatgccgg
    10741 atgcggcgta aacgccttat ccagcctaca aattgccaaa attcaatata ttgcagtgac
    10801 tgcgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgtcagc agtcagaagc
    10861 accttgtgaa aggtgcttaa tagattattt tccagcgaac aactttggaa tttcccgcag
    10921 acaccaggat ttcgcttccc ccatactgtc acgacgccag gccataataa tatcgatctc
    10981 gctggtcgat tccgggctga cgacacgcaa ccgcccttcc gcaatatctt tttcgaccat
    11041 cggatacggc atcgtcgcca cgccaagccc cgccagtaat gcctgacgtt tatcttcaat
    11101 cgtgctcacc gttaagcgcg gctgtttgtc cagcagctgt acggtcaaca ccgggcgctc
    11161 acgagcggta tccgctaccg caattccacg atatttcaca cgcgtcactt cagataacgg
    11221 ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg tagacgttca ttaacgtata
    11281 gagcttgcgc gagttgatct ccgacgagga acgaaaatgc atatccggcg cgataacgat
    11341 atccgcccgc ccctgctcca gccgttccca cgcccccgcc agcacttctg tgatgattgc
    11401 cagttgggta ttggcttttg ccgccagttt gtcgattaac gggaaaaagg caggtgtcgg
    11461 taccagcgct tcggtcacaa tggtgagatg cgtttcccaa ccgcgcgcga gagcttccgc
    11521 atcagtagtc agtttatctg cggcttccag caaaacgcgc cctcgctcca gcaacatcct
    11581 cccgacattg gtgaatttgg tgcgatggcc cgagcggtca aacagcacca catccagctc
    11641 ttcttccagt ttttgcatga tgtagctaag tgcggaaggc acgcgtccca gctcatccgc
    11701 tgccgccgca aaactgcccc ggcgatcgat cgcatccata acccgtagtg cttccagcgt
    11761 taatgccctt tctttggcca tttcgttctc attcaggaaa tttgaacata ccgggcagaa
    11821 tatctggcta acaatgtagc gtccagcccc ttaccataaa aggaagtaaa gagaggtcaa
    11881 gaattatgat tactacccga actgccaggc agtgtggaca agcagactac ggatggttgc
    11941 aggcccggta tactttttcc tttggacact acttcgaccc gaaattgtta ggctatgcct
    12001 ccctgcgtgt gcttaaccag gaagtgctgg ccccaggtgc cgcctttcag ccgcgaacct
    12061 atcccaaagt cgatatttta aatgtgattc tggatggaga agcagagtat cgcgacagcg
    12121 aaggcaatca tgttcaggcc agcgccggag aggcgttgct gctctctacc cagccgggtg
    12181 tcagctatag cgaacacaat ctcagcaaag acaaaccgtt aacgcgaatg cagctttggc
    12241 tggatacctg cccgcagcga gagaatccgc tgattcaaaa gctggcgctt aatatggaca
    12301 agcagcaatt aatcgcctcg ccagagggtg cgatgggaag cctgcaatta cgccaacaag
    12361 tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc gaatttccag ttgcatgggc
    12421 cacgcgcgta tttgcaatcg attcacggga aatttcatgc gcttacgcat catgaagaga
    12481 aagcggcgct aacctgcggt gatggggcgt ttattcgtga cgaggctaac attacgctgg
    12541 ttgccgattc cccactgcgc gctttgctga tagatttgcc tgtctagttg tttttacagg
    12601 gagatgatga tgagtaaaaa atcgaccaaa aagcgccagc tagcgaagcc cgtggtggcg
    12661 aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag agatgttgag cgagctggaa
    12721 gctatcgtcg cggatgctga aacgcgttta gccgaggatg aagctaccgc gtaaagacag
    12781 gttgccggat gcggcgtaaa cgccttatcc ggcctacaaa acacaacaac atcaacaaat
    12841 taaacacccc gtaggccgga taagatgcgc cagcatcgca tccggcatca ggtacaaaac
    12901 acaacaacat caacaagttg agcaccccgt aggccggata aggtgcgcca gcatcgcatc
    12961 cggcacaata ctcaaaacct ggcgtttatc tggccttgct cgccataatc tcgataatct
    13021 gccgatccgt ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct
    13081 caacatcatg cgccacgatc ccttcattgc cggtcacggc ggtatcatcc agcgccatta
    13141 acaccgcttt ccacgcagcc gaagcactgg tcgaaacctt cgcaccatcg caaatcatgc
    13201 cgctgacatc gccgatcata ctgctgatcg ccatcgagat agtttcataa cgcccatcca
    13261 ccagccatgc catcccggcg gcggccccca ttgctgcggt cgttgcggca caaagtgcag
    13321 acaaacgcgg taactggtta tggatgtaaa ttgcgctcaa atgagaaagc atcagcgcac
    13381 gcgccagtcg ttcatcatcc gctccgaagt gttctgctac aaccaccaca ggcattgttg
    13441 cggtgatccc ctggttaccc gagccggagt tactcatagc cggaagcgta gcgccgccca
    13501 tacgcgcatc ggatgccgcg ctggtacgaa tcacaatgga tgaagagaga tctttcgcca
    13561 gcaaaccgcg ctcgcactgt ttttccagcg tcgcgccaat atgcagcccc cacttaccgc
    13621 tcaaaccttc ctgcgataac gcacaattta gcttcgcgga atcgagaata aagcggatcg
    13681 ccgcaaatgg gacttcattg acgaacttca ggatctcagc cagcgtcgtt ctggaaagca
    13741 ccgtcagcgg cgactcttgc tcgccctctg ccacacacgc ctgctgggta aacaccacac
    13801 cattgtgcgt ctcaatatgc acaatgttgg tatgcccgcc gacgatggtg acacacgccc
    13861 acttctcacc gttccagact ttggcgcgtg agaagaggat ttcattgcaa ggttcctgga
    13921 tcttaacaga gactttcccc gccgccagca gtgctttggc atcggcaatt gcctgcgcag
    13981 ttgcgtcttt cagcacttcc agcccggcgt tggcatttcc acctaacgcc cccagcgccg
    14041 ccgcaatcgg cagccccacc attcccgtgc cgggaacggt gatgcccaga ccgttcttca
    14101 tcagatttgg cgaaacccag gcttctacac gttcaaccgg accttccagt tctgccgcag
    14161 caaccgccgc cgccagcgcc agtgaaatcg gttcagtaca tcccagcgcc ggttttactt
    14221 cctcctgaac ggcgaggatg taacgctgcc ataacggatt taaagtcgaa tcaaacatat
    14281 taaaaacctt aaaatttcag gtaaatcaag aaaatgccag gaacagagaa acacaaagca
    14341 acaaaccagt gacgataatc aggtacagag acatcccttt gtatttgtgc aatgccggta
    14401 ctttgtaaac cagccacgcc gggatcaggc accctaccat gccgaaaatc gggctacaga
    14461 tagaggtgaa gctcaacacc ggtgcgttca gtacgatggc gctccaggcc agcaaaatgg
    14521 cgaaaatcat gatgccgcgc tgaacaagat tttcgttaat cttctcggca ggcatcttgc
    14581 gacgcaggat gttcattacg atcccttgcg ttgcttcgcg aaagcctaaa tagacgccaa
    14641 agaatgcagt catgacagca aagatattga gaatgacgct gaccactttc acccacgctg
    14701 cgccgtcacc gctaataaac tgtgcggcaa tcgccagcgc ggaaatattc tgctcgtagg
    14761 cttttaccgc ttcgtcatgt cccatcgcca gcgtgaacga cacggcgtag aaaaagacgg
    14821 tgacaaacaa aatgccaaac gcgatattca ttgcccgcaa tgctttatgc cgcgccactt
    14881 caattgattt ttcccgcgag cgataagaga tcaccatcgg gcttaacgtc tggataaaca
    14941 gaatcgacgt cagggtaaac ggcagcgtaa taatggcgtt tttcaccagc agccccagcg
    15001 gcggtagcga accgacgtta tacagatgcc acattcccac catcgacacg cccagcgcag
    15061 cgactaccag cagcttggtc agcaccatgt cggtcgaaat tttgaataac aatttctcgc
    15121 cgcgtgaaga gatcgccacc agaatgcaaa tcagtaccag accataaaag ggactgtctg
    15181 acagcaaccc ttccgtcacg ccgaaggtat gcagatagga agcactatcg ttggtgatgg
    15241 cggtggaata gacgaacatc caaatcacca gcattacgaa atagagcgca cctaacagga
    15301 tgccccagtt tttacctaaa taaccgctaa tgacactcgg gtaatcttta cattctggtg
    15361 attctgccag cgtattaata aacaaccgct gaaacagata cattgccggg taaccaatca
    15421 ccgatgagag caaaaatacc cacaatccca tcaaaccgac ctgcaccggg agaaaaacaa
    15481 tccccgcgcc aatcgccatc ccgatactca taatcaccca gcaggtgtca gtactgtcga
    15541 atttgatcgc ttctcgccac tcgctctcac tcattccagc acgacccgcc ggggtcgaag
    15601 cgtctgcaat gacgccttta ttcgatgcaa tttccataat ttctcgctca atattttgta
    15661 gggcttatta tttttttccg agccgcatca aggcgatatg cggtctacgt ttttgcaggc
    15721 gagttattag aataaagaaa tgatacgcgc gaggtcagag aaaatcttca caatcaagac
    15781 tttaaaataa cagagatgga taagaatttt ctactaaatt aatcgcaaga gaatattcat
    15841 tctctatttg cggcagatca caaaaaaaag gtgcac
//
LOCUS       DAJVQH010000092        15829 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJVQH010000092 DAJVQH010000000
VERSION     DAJVQH010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.092"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..882)
                     /locus_tag="OQM87_002923"
     CDS             complement(<1..882)
                     /locus_tag="OQM87_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1508990.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGA"
     gene            989..1285
                     /locus_tag="OQM87_002924"
     CDS             989..1285
                     /locus_tag="OQM87_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1508991.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="OQM87_002925"
     CDS             complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="OQM87_002925"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCS1508992.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="OQM87_002926"
     CDS             complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="OQM87_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCS1508993.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="OQM87_002927"
     CDS             complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="OQM87_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCS1508994.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4298..4381
                     /locus_tag="OQM87_002928"
     CDS             4298..4381
                     /locus_tag="OQM87_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCS1508995.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4435..5787)
                     /gene="gorA"
                     /locus_tag="OQM87_002929"
     CDS             complement(4435..5787)
                     /gene="gorA"
                     /locus_tag="OQM87_002929"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCS1508996.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5859..6701)
                     /gene="rlmJ"
                     /locus_tag="OQM87_002930"
     CDS             complement(5859..6701)
                     /gene="rlmJ"
                     /locus_tag="OQM87_002930"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCS1508997.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6904..8946
                     /gene="prlC"
                     /locus_tag="OQM87_002931"
     CDS             6904..8946
                     /gene="prlC"
                     /locus_tag="OQM87_002931"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCS1508998.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRSGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8954..9706
                     /gene="rsmJ"
                     /locus_tag="OQM87_002932"
     CDS             8954..9706
                     /gene="rsmJ"
                     /locus_tag="OQM87_002932"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCS1508999.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9755..11224)
                     /gene="dtpB"
                     /locus_tag="OQM87_002933"
                     /pseudo
     CDS             complement(9755..11224)
                     /gene="dtpB"
                     /locus_tag="OQM87_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(11530..11964)
                     /gene="uspA"
                     /locus_tag="OQM87_002934"
     CDS             complement(11530..11964)
                     /gene="uspA"
                     /locus_tag="OQM87_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCS1509000.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12355..12690
                     /gene="uspB"
                     /locus_tag="OQM87_002935"
     CDS             12355..12690
                     /gene="uspB"
                     /locus_tag="OQM87_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCS1509001.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12761..14260)
                     /gene="pitA"
                     /locus_tag="OQM87_002936"
     CDS             complement(12761..14260)
                     /gene="pitA"
                     /locus_tag="OQM87_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCS1509002.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14492..15691
                     /locus_tag="OQM87_002937"
     CDS             14492..15691
                     /locus_tag="OQM87_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCS1509003.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPVLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITT
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3982 a         4048 c         4154 g         3645 t
ORIGIN      
        1 tgcgccggca ctgccgatga ttaatgccat taatgctccc agacccattc cttttgtcat
       61 cagaacagat gccagaggga taaccgcctc agcccggata tacagcggaa taccgacaac
      121 ggcgcttaat gggatggcga agggattatc tgctcctgca tgagcggcaa tccacgctga
      181 gggaataaag ccataaataa aagatcctat taatactcct aacaaaagat atggcagtac
      241 atctttaaac tgttgcaacg catctttggt ggctagtttt atgggattaa tacttactac
      301 agctttggta ctgcaacagg taactacggg gttctcaatg gctttagccg ctggactaca
      361 acaactcact tttatgggcg tatatgtcgt tcccggcgaa gtttttgctg gagcacaaca
      421 atttgctgat gagcttttac tggcaatgat atgacgttca aaccccagag aatccaggat
      481 aatactggca agaacggaga cgccggctgc gataatcgcg tacaacaagg tgactttcca
      541 accaaaggtc atccacatta gtccgacgat aatgggatta agtaatggtg aaacaaataa
      601 aaaagtgaga gtcggaccaa acccggcttt cgctgacaac aatccacgta acatgggtat
      661 tgtcgagcaa ctacagaacg gggtaacggc tcccaacaga gcagccagga gatagcctct
      721 cccttttctg gcccccatca tctgctggat tttatggtct ggcacctttt gtcttatcag
      781 gctgacaccg gcactaatca ctataaacag caacgaaagc tcgacggcga gaaacacaaa
      841 catctctgcg gcatcttgca gcatcgcaag ccaactactc atacgtaacc tcattgggta
      901 aatctatatt tccagaataa tcgaaatgaa attcagatca agatatttct ggtattatcg
      961 aaatatcaac cagacagagg ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa
     1021 agagctaggt cacccgaccc gtttgttcat atttaaacat cttgtcaaag ccggggaaca
     1081 agggttgccg gtcggtgagt tgcaaaagca gcttgggata cctggctcca cactcagcca
     1141 ccatatatca gcactggtat cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct
     1201 gatgtgtgtt tcgcaatacg caatgctgga agcgataatt gaattcttac gcgaggagtg
     1261 ctgcgtaaat agcaaaattg cctgatgcgc tacgctctac atatttcatg ccatatattg
     1321 aatttgcacg gttttgtagg tcggataaga cgcgttagcg tcgcatccga cataaacaaa
     1381 gcgcacttta ccaactatcg gcagtggata tcgccgcttt atttcaggcg cttacccgct
     1441 tcatcaacca ctttctcgcc atcttccttg gagaatgcgc ctttttgcgc atctggcaga
     1501 atttccagca ccacttctga agggcggcac aggcgagttc ccagcggcgt caccacaatc
     1561 gggcgattaa tcagaatcgg gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat
     1621 ttatcttccg caaggcccag ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt
     1681 acggaaatcc ccatatcggc aatgagtttg accagttcat cgcgcgttgg cggagtttcc
     1741 agataatgga taatggtcgg ttctgtgccg ctgttgcgga tcatctccag cgtattacgc
     1801 gacgtgccgc aggccgggtt gtgataaatg gtaatgttgc tcatatcagt atctcattac
     1861 aaagtgaaag agagacgtag cgccagcgca gccagcgtca caaacagcac aggcagggtc
     1921 atgataatcc ctgtacggaa gtaatatccc cagctgatag tcatattctt ctgcgaaagt
     1981 acgtgcagcc agagcagcgt tgccaggcta ccaatcgggg taattttcgg ccccaaatcg
     2041 cagccaatca cgttggcata aaccatcgct tctttgataa cgccagatgc cgtgctgcca
     2101 tcaatggaca acgcgccaac cagtaccgtc ggcatattgt tcataataga agagaggaag
     2161 gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg
     2221 agtacgccag aaagatattc cgttaatccg gcattgcgca ggccataaac caccagatac
     2281 atgccgagcg agaagatgac aatctgccag ggggcaccgc gcaggacttt acccgtatta
     2341 atcgcatgac cgcgtttagc gacgacaaat aatatcagtg cgcccacagc tgcaatggcg
     2401 ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca ccagcagaag cagtaaaaca
     2461 acccagccag ttttgaacgt agcaggatct ttgatcgctt ctgcgggaga tttcagcagc
     2521 gccatatcgt agttctgcgg aatatctttg cgaaaataga gatgtagcat caccagcgtg
     2581 gcaacaatcg cggcgatatc caccggcacc atcaccgagg cgtattcgcg aaagccgagg
     2641 ccaaagaaat cagcggaaac gatattcacc aagttggaga caataagcgg caggctggcg
     2701 gtatcggcaa tgaatccggc cgccatcacg aacgccagcg tcgtgccttt actgaatcct
     2761 aaagccagca gcatggcgat gacaatcggc gtcaaaataa gcgccgcgcc atcattggca
     2821 aacagggccg caacggcggc accgagcagg acaatccagg taaacagcaa gcgaccgcgt
     2881 ccattgcccc agcgtgaaac gtgcagcgct gcccattcaa aaaagccgga ctcatccagc
     2941 agcaggctga tgataatgac ggcaataaac gccgccgtcg cgttccagac gatattccac
     3001 accaccggaa tatcacccgg atgaaccacg cccgtaacca acgccagtac cgcgccgagc
     3061 gttgcactcc agccgatgcc taaacctttc ggctgccaga taaccaatac gatggtcagg
     3121 acaaagatag cgcctgccag taacataatg cctcccggat aaaacacatg tgcaaatcca
     3181 tatgtgttta gctaaatttt taactgcaaa tgttcttact gtctccggaa cagttttgtc
     3241 tggccaggtt gcggacaatt gcctgaatct tttcctgttc acatcgccag gcttcatcaa
     3301 taattttcgc cgcccatgct ggaatatgcg gcgataagcg gtaatgaacc cacttacctt
     3361 gcttgcggtc cagcaatagc ccgctttcac gcagcaatgc cagatggcgg gatatcttgg
     3421 gctgcgactg atcgagggca gtgcagagat cgcagacgca taactctccc agttcgctaa
     3481 gcagtaaaac gatgcccaga cgggtttcat cagcaagaat tttgaacaat tggatgggta
     3541 acagaaatga catattgcgc tcctgattgt tgcaggtagt gtctctcttc gaagcggata
     3601 agtcaaaaac atatatgact taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa
     3661 ttttgagttg gctattcatt tgaaaggcgg tttgaatcag gacgtaaaaa aaaccgactt
     3721 tgcgtcggtt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
     3781 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
     3841 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
     3901 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
     3961 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
     4021 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
     4081 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
     4141 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
     4201 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
     4261 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
     4321 tcttggggcg ttaattgtgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
     4381 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
     4441 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
     4501 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     4561 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     4621 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     4681 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     4741 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     4801 cttgttatta aacaagcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     4861 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     4921 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     4981 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     5041 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     5101 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     5161 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     5221 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     5281 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     5341 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     5401 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     5461 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     5521 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     5581 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     5641 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     5701 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     5761 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     5821 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     5881 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     5941 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     6001 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     6061 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     6121 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     6181 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     6241 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     6301 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     6361 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     6421 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     6481 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     6541 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     6601 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     6661 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     6721 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     6781 aggccgccgc cgcgaaagcg taacgtttcc cattgaaatt cactacactt aaccccatgc
     6841 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     6901 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     6961 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     7021 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     7081 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     7141 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     7201 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     7261 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     7321 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     7381 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     7441 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     7501 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     7561 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     7621 agcacccgcg cctccgatca aggcccgaac gccggtaagt gggataacag caaggtgatg
     7681 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     7741 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     7801 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     7861 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     7921 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     7981 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     8041 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     8101 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcagc
     8161 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     8221 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     8281 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     8341 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     8401 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     8461 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     8521 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     8581 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     8641 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     8701 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     8761 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     8821 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     8881 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     8941 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     9001 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     9061 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     9121 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     9181 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     9241 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     9301 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     9361 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     9421 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     9481 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     9541 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     9601 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     9661 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     9721 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     9781 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     9841 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     9901 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     9961 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
    10021 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
    10081 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagtcg
    10141 tcaaaaagcc cagtgagcac ataaacatgc cgagagtaaa tttcatcggc atcgagaggt
    10201 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
    10261 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
    10321 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
    10381 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
    10441 tatccagctt gaatgcctga cgaaagaaga tgatggtgac gacgatggag agaacaatca
    10501 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
    10561 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
    10621 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
    10681 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
    10741 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
    10801 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
    10861 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
    10921 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
    10981 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
    11041 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
    11101 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
    11161 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
    11221 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
    11281 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
    11341 tattacaaca gaaaataacc agctgatcac actaatgatg gtgttaaatt ttgtattggc
    11401 aggagatttt gattgcgtac gcagatcgta ggccagataa ggggtttacg ctgcatcagg
    11461 taacaaggcg cgccaaaaag caaaacgccc gcatatgcgg gcgttcagga acacgtcgtg
    11521 gagggatgat tattcttctt cgtcgcgcag cggaacaatc agcatatcaa cgtgaacggt
    11581 gttgatcagc tgacgtgcgg aagacatcag tttgctccag aagtcctggt ggtgaccaca
    11641 aaccaccaga tccatatcgt atttcttgat tgcatcgacc agaacctggc ccaggtcgcc
    11701 gctgccgctc agggtttcag tgattggata gcctgcatta gtggaaagct cggtcagcgc
    11761 atgatgtgtc tcttcagaga tgcgtttctg catatcaccc agattcacat caataagccc
    11821 ggtgtatagg tcagagtagt ttacatctac gtggatcaga gaaactttcg cattgtaggg
    11881 gcgagccata gagactgctt tctctaccag aactttgctt tccggggaga gatcgactgc
    11941 gatgagaatg tgtttataag ccatagtgtt actccttcca taaagttgtc gatgactggc
    12001 cagctagcgt ttcttgtgcc gttacgtcac ccgcgtcctg cgaactacgt gcgcggggct
    12061 aacccagccg taaagattca gtgttaagac catccttacc ttatagcgac ccggatgatc
    12121 cgtcaatccg ccttgcttac ccgttagata aacaaatttt cgacatgaat atattgatag
    12181 tggttaacct tctggaaaaa aaacaacctg atctcctaca ctatctatag agccgctcgg
    12241 atgttggtca ccacaaaagc ggtttctcca ggacctggtg ttaactgatt ggcagcgtat
    12301 cgggagcggt agccccgggg tggaactccg cgggcaggtc gccggggagg aggtatgata
    12361 agcaccgtcg cattattttg ggctttatgt gtcgtttgca ttgttaacat ggcgcgctat
    12421 ttctcatcac tacgcgcgtt gttagtggta ctgcgtaact gcgatccatt gctctatcaa
    12481 tatgttgatg gagggggctt ttttacctca catggccaac ccaacaaaca ggtgcgtctc
    12541 gtttggtata tctatgccca acgttatcgc gatcatcacg atgatgagtt tattcgccgc
    12601 tgtgagcggg tgcgtcggca gtttattctg actagcgcat tgtgtggtct ggtggtggtc
    12661 agcctgattg cattgatgat ttggcattaa gcacttcagc aataataacg cataaaaaaa
    12721 gcgggccagt aaactgaccc gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga
    12781 accagtacag cccgccggaa agtagtaccg cagccggaag ggtaaacacc caagccatca
    12841 gaatactggt tacggtttta cgctgtaagc cgccgccatc taccaccatc gtccccgcga
    12901 cagaagagga gagtacgtga gtggtggaaa ccggcatccc ggtataactc gccaggccga
    12961 ttgacactgc cgccgtcatc tgggcagaca tcccctgagc gtaggtcatg cctttcttac
    13021 cgattttctc accgatagtc gttgccacac gacgccagcc aatcatcgta ccgataccta
    13081 acgccagcgc gaccgccatg atgatccaca ccggtgcata ctcgatggtg ctaagcatgt
    13141 cggacttcag tttcttcaac aggcgctgat cgtcagcact cacaccaggc atcttcacca
    13201 ctttgtcgat agtgtcagaa acgcacagca taatgcggcg catctggcta cgttgatcaa
    13261 gcgacagctt gtcgtagctt tccacatcgg tggtcaacat acctttcagg cggttgagcg
    13321 cgttaatggt attcgacgga tggcagtgga actccgcagg ttgcgttgcg ccagcttccg
    13381 gagccggtac taactgatca gcaccggtag cctgtttgag cagcgcagga tgctgctcaa
    13441 agtaagcttc gacgttgttg atggcatcac gggtacgggt gatttcgtag ccagtggcat
    13501 tcatgttcac cacgaagcct gctggcgcga cgccaatcaa taccaacata accagaccaa
    13561 tgcctttctg accatcgttc gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca
    13621 gcgcgatacg cgtccagaac ggcggctttt tcttgccgtc tttcttttca cgctccgctg
    13681 gggtcaggtg gatacgggcg cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa
    13741 tcagaccgcc agcaaacacc aggccgacaa taggggaaac gatcagagaa ccgaaaatac
    13801 ttaatacttt cgggatattg agtgcatcca ccactgacgt cccggtcatc aacgcattgg
    13861 ttaaaccaat cccgatgatc gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa
    13921 agtaccaggt acccaggttc cagataatcg ccgccagcaa catagagaac accatggcaa
    13981 ggccatgaga cgatcccata ttaagcagca gatccgtcgg cagcatatgc acaatggcat
    14041 aggcaacact cagaccaccc agcaaaacac ccaaaaagtt gaacaccgcc gccataacca
    14101 cggcgagctg agaacgcatc gcgcgggtat agataacggt tgccacggcg ttggctgtgt
    14161 catggaaacc attgatggct tcgtagaaca gcacaaaagc cagtgcaagc aataataaca
    14221 gcccggtatg caaatccagg ccagcaaaca aatgtagcat aggacgttac gccattttga
    14281 ggacatgaac gcggcgcatt atcagtgact ttcgcggcgc gggcaaagtg aaatatagac
    14341 tttttttgat ttgcctcctg tatggatttc actcaaaaaa taattatctt atataattca
    14401 ggcaaatact tccttttagt aatattgatg ctggtgcgac cactgaggaa tctttacaat
    14461 tcacgcccgt tttttctaag aggagcgcaa cgtggaaagg tttgatgcca ttattatagg
    14521 cgctggtgcg gcgggtatgt tctgttctgc gctggcaggt caggcaggac gccgggttct
    14581 gctgatcgat aatggtaaaa tactagggcg caaaatcctt atgtctggcg gtgggcgctg
    14641 caactttacc aacctttatg tcgaaccagg cgcttatctg agccagaatc cgcatttttg
    14701 taagtctgca ctcgcacgtt ttacccagtg ggatttcatt gatctggtca ataaacacgg
    14761 tatcgcctgg cacgagaaaa cgttagggca actcttctgc gatgactccg cgcagcagat
    14821 tgtcgacatg ctggtggatg agtgcgagaa gggcaatgta accttcagat tgcgtagcga
    14881 agtgctgagt gtggcgaagg atgaaacagg cttcacgctt gatctgaacg gcatgactgt
    14941 cggttgcgaa aagctggtca tcgcgaccgg tgggctgtca atgccggtgc tgggcgcatc
    15001 gccgtttggt tataagattg ccgaacaatt tggcctcaac gtgctgccga cccgcgcggg
    15061 tctggtgcca ttcactctgc ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt
    15121 ggcggtgcct tccgtgatta ccactgaaaa cggcaccgtt ttccgtgaga acttactctt
    15181 cacccaccgc ggcttatctg gaccggcggt gttgcagatt tcaagctact ggcaaccggg
    15241 tgaatttgtc agtatcaatc tgctaccgga tgtggacctc gaaaccttcc tgaatgagca
    15301 gcgtaacgca catccgaatc aaagcctgaa aaacacactg gcggttcatc taccgaagcg
    15361 gttggttgaa cgcttacagc aactcgggca aatcccggat gtttcgctaa aacagctcaa
    15421 cgtgtgtgac caacaggcac tgattagcac attgaccgac tggcgcgtac aacccaacgg
    15481 cactgaaggc tatcgcactg ccgaagtgac gctcggcggc gtggacacca acgaactttc
    15541 ttcacggacg atggaagcgc gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga
    15601 cgttaccggc tggctggggg gctataactt ccagtgggcg tggtcgagtg catgggcttg
    15661 tgcgcaggat ttggcgtcaa ttgtgaaata accatgacgt tttcccgtca gccgtttgta
    15721 tatgtcgtta ttaagtcatt ttggtacggt tctgcatccg acattctggt gttaactcgc
    15781 tgttttgtta gcgaattaac gctattgccg ttgtggttat tttatagtg
//
LOCUS       DAJVQH010000093        15668 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJVQH010000093 DAJVQH010000000
VERSION     DAJVQH010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.093"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..2945
                     /gene="mdtB"
                     /locus_tag="OQM87_002938"
     CDS             <1..2945
                     /gene="mdtB"
                     /locus_tag="OQM87_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCS1509004.1"
                     /translation="SAVTAPLERQFGQMSGLKQMSSQSSGGASVITLQFQLTLPLNVA
                     EQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPMTQVEDMVETRV
                     AQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAITGANVNSAKGSL
                     DGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAENSWLGAWANKE
                     QAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTTNIRASVDDTQF
                     ELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFSINNLTLMALTI
                     ATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFSLIAVLIPLLFM
                     GDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQNRFSRASEKMFD
                     RIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVALSTLLLSVLLWVFIPKGFFPVQDNG
                     IIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQIN
                     LKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQATSL
                     DALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNALY
                     NAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKIEQ
                     RFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGSTLA
                     FQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGSEL
                     DVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTTLA
                     ALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLVLWTKSR
                     FARHEEEA"
     gene            2946..6011
                     /gene="mdtC"
                     /locus_tag="OQM87_002939"
     CDS             2946..6011
                     /gene="mdtC"
                     /locus_tag="OQM87_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCS1509005.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLRITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            6012..7427
                     /locus_tag="OQM87_002940"
     CDS             6012..7427
                     /locus_tag="OQM87_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1509006.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            7424..8827
                     /gene="baeS"
                     /locus_tag="OQM87_002941"
     CDS             7424..8827
                     /gene="baeS"
                     /locus_tag="OQM87_002941"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCS1509007.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            8824..9546
                     /gene="baeR"
                     /locus_tag="OQM87_002942"
     CDS             8824..9546
                     /gene="baeR"
                     /locus_tag="OQM87_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCS1509008.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            9737..10069
                     /locus_tag="OQM87_002943"
     CDS             9737..10069
                     /locus_tag="OQM87_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCS1509009.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            10216..11577
                     /gene="yegQ"
                     /locus_tag="OQM87_002944"
     CDS             10216..11577
                     /gene="yegQ"
                     /locus_tag="OQM87_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCS1509010.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(11919..12236)
                     /locus_tag="OQM87_002945"
     CDS             complement(11919..12236)
                     /locus_tag="OQM87_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509011.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            12642..13541
                     /gene="yegS"
                     /locus_tag="OQM87_002946"
     CDS             12642..13541
                     /gene="yegS"
                     /locus_tag="OQM87_002946"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCS1509012.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(13623..14396)
                     /locus_tag="OQM87_002947"
     CDS             complement(13623..14396)
                     /locus_tag="OQM87_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1509013.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(14502..15542)
                     /gene="gatD"
                     /locus_tag="OQM87_002948"
     CDS             complement(14502..15542)
                     /gene="gatD"
                     /locus_tag="OQM87_002948"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1509014.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
BASE COUNT         3592 a         4182 c         4099 g         3795 t
ORIGIN      
        1 cctctgccgt taccgcgccg ctcgaacgcc agttcgggca gatgtctggc ctgaaacaga
       61 tgtcgtcgca aagttccggc ggtgcgtcag ttatcacttt gcagttccag ctaacattac
      121 cgctcaatgt cgccgagcag gaagtgcagg ccgcgattaa cgctgcgacc aacttgttgc
      181 cgagcgatct gcctaacccg ccggtttaca gcaaagtgaa cccggcagat ccgccgatca
      241 tgacgctcgc cgtcacctca accgccatgc cgatgacgca agtggaagat atggtggaaa
      301 cccgcgtcgc gcagaaaatc tcgcagattt ccggcgtcgg cctggtgacg ctttccggcg
      361 gtcagcgtcc ggctgttcgc gtcaaactta acgctcaggc gattgccgcc ctcggcctga
      421 ccagcgaaac cgtgcgcacc gccattaccg gcgctaacgt taactcggca aaaggtagcc
      481 tcgacggccc ttcccgtgcg gtcacgcttt ccgcgaacga ccagatgcaa tccgccgaag
      541 agtatcgcca gctaatcatc gcctaccaga acggcgcgcc aattcgtctg ggcgatgtag
      601 caactgtaga gcaaggtgca gaaaacagct ggctcggcgc gtgggcgaac aaagaacagg
      661 ccattgtgat gaatgttcag cgccagcccg gtgctaacat tatctccacc gccgacagca
      721 ttcggcagat gctgccacag ctcactgaga gtctgccgaa atcggtgaag gtgacagtgc
      781 tttccgatcg caccaccaat atccgcgcat ccgtcgatga tactcagttt gaattgatga
      841 tggctatcgc gctggtagtc atgattatct acctgttttt gcgcaatatt ccggcgacca
      901 tcattcccgg tgttgctgta ccgctgtcgt taatcggcac tttcgcggtt atggtgtttc
      961 tcgatttttc aatcaataac ctgacactga tggcgttaac tatcgccacc ggattcgtgg
     1021 tcgatgacgc catcgtggtg atcgaaaaca tttcccgcta tatcgaaaaa ggcgaaaaac
     1081 cgttggcggc ggcgctcaag ggcgcaggtg aaatcggctt taccattatc tcgctgacct
     1141 tctcactgat tgcggtgttg atcccactgc tgtttatggg cgatatcgtc gggcgactgt
     1201 tccgcgaatt tgctattacc ctggcggtag cgattttgat ctcagcggtg gtgtcgctga
     1261 ccctgacacc gatgatgtgc gcgcggatgc tcagccagga gtcgttgcgt aaacagaacc
     1321 gcttctcccg tgcctcggaa aaaatgttcg acaggataat cgccgcctat ggtcgtggac
     1381 tggcgaaagt gctgaatcat ccgtggctga ccttaagcgt ggcactcagc acgctgctgc
     1441 ttagcgtggc actcagcacg ctgctgctta gcgtgctgct gtgggtgttc attccgaaag
     1501 gtttcttccc ggtacaggac aacggcatta ttcagggcac tttgcaggca ccgcaatcca
     1561 gctcctttgc caatatggcc cagcgacaac gccaggtcgc ggacgtgatt ttgcaggatc
     1621 cggcagtgca aagcctgacc tcatttgttg gcgttgatgg cactaacccg tcgctgaata
     1681 gtgcacgttt acaaatcaac ctcaaaccgt tggatgaacg tgatgatcgg gtgcaaaaag
     1741 tcatcgcccg tctgcaaacg gcggtagata aagtgccggg cgtcgatctc ttcctgcaac
     1801 caacgcagga cctgaccatc gatactcagg tcagccgcac ccagtaccag tttaccttgc
     1861 aggccacgtc actggatgcg ctcagtacct gggtgccaca gttgatggaa aaactccagc
     1921 aactgccaca gctttctgat gtctccagcg actggcagga caaagggctg gtggcgtatg
     1981 tcaatgttga tcgcgacagc gccagccgtc tggggatcag catggcggac gtcgataacg
     2041 ccctgtacaa cgcgtttggt cagcgactga tttccactat ttatactcag gctaaccagt
     2101 atcgcgtggt gctggagcac aataccgaaa ataccccagg cctcgcggcg ctggatacca
     2161 ttcgcctgac cagcagcgac ggtggcgtgg tgccgttaag ctcaattgcc aaaattgagc
     2221 agcgttttgc gccgctttcc atcaaccatc tggatcagtt cccggtaaca accatctcct
     2281 ttaacgtgcc ggataactat tcgctgggcg atgcggtgca ggcgattatg gacaccgaaa
     2341 agacgctgaa tctgccggtg gatatcacca cgcaattcca aggcagcacc ctcgccttcc
     2401 agtcggcgct gggcagcact gtctggctga ttgtcgcggc ggtggtggcg atgtatatcg
     2461 tgctcggcat tctgtacgag agctttattc acccgatcac cattctctcg acgctaccca
     2521 ccgcaggggt cggcgcgctg ctggcattaa tgattgccgg tagcgaactg gatgtgattg
     2581 cgattatcgg cattattttg ctgatcggta ttgtgaagaa gaacgccatc atgatgatcg
     2641 acttcgcgct ggctgctgag cgcgagcaag gcatgtcgcc gcgcgatgca atctaccagg
     2701 cttgtctgtt gcgttttcgt ccgatcctga tgaccaccct ggcggctctg cttggcgcgc
     2761 tgccgctgat gttgagtacc ggggtcggcg cggaactgcg tcgtccgtta ggtatcggca
     2821 tggtcggcgg tctgattgtc agccaggtgc tgacgctgtt caccacgccg gtgatttatc
     2881 tgctgttcga ccgcctggta ttgtggacca aaagtcgctt tgctcgccat gaagaggagg
     2941 cgtaagtgaa gttttttgcc ctcttcattt accgcccggt ggcgacgatt ttactgtcgg
     3001 tcgccattac cctgtgcggc atactgggtt tccgtatgct gccggtcgcc ccgctgccgc
     3061 aggtcgattt tccggtgatt atggtcagcg cctcgctgcc cggtgcgtcg ccggagacaa
     3121 tggcgtcttc cgttgccacg ccgctggagc gctcacttgg gcgcattgcc ggggttagtg
     3181 aaatgacctc cagcagttcg ctcggcagca cgcgcattat tttgcagttt gattttgacc
     3241 gggatatcaa cggcgcagcg cgtgatgtgc aggcggcgat caacgctgca caaagtttgc
     3301 tgcccagcgg gatgcccagc cgcccgacct atcgcaaagc gaacccgtcg gatgcgccaa
     3361 ttatgatcct cacgctgaca tccgatactt attcgcaggg tgaactgtac gatttcgcct
     3421 cgacgcagct ggctccgacg atttcgcaaa tcgacggtgt cggtgatgtc gatgtcggtg
     3481 gcagctcgct gcccgccgtg cgcgtcgggc tgaacccgca ggcgctgttt aatcagggcg
     3541 tgtcgctgga cgacgtacgc actgccatca gcaatgccaa cgtgcgtaaa ccgcaggggg
     3601 cgctggaaga tggcactcac cgctggcaga tccagaccaa cgatgaactg aaaaccgccg
     3661 ctgaatatca gccgctgatt attcactaca acaacggcgg cgcggttcgt ctgggcgatg
     3721 tggcgacggt gactgactca gtgcaggatg tgcgcaacgc cgggatgacc aacgccaaac
     3781 cggctatttt actgatgatc cgcaaactgc cggaagccaa tattatccag acggtagaca
     3841 gcatccgggc aaaattacca gagttgcagg agaccattcc ggcggcgatt gatctgcaaa
     3901 ttgcccagga tcgctccccc accattcgcg cctcgctgga agaagtcgag caaacgctga
     3961 ttatctcggt ggcgctggtg attctggtgg tctttttatt cctgcgctcg ggtcgcgcca
     4021 ctattattcc cgccgttgcg gtgccggttt cgctgattgg tacgtttgcg gcgatgtacc
     4081 tgtgcggatt cagtctcaat aatctttcgc tgatggcgct caccattgct actggtttcg
     4141 tggtggatga cgccattgtg gtgctggaaa acattgcccg tcatctggaa gcggggatga
     4201 aaccactaca ggccgcgctg caaggtactc gcgaagtcgg ctttacggtg ctgtcgatga
     4261 gtctgtcact ggtggcggtg ttcctgccgc tgctgttgat gggcggattg ccgggccgac
     4321 tgttacgcga gtttgccgtg acgctttctg tcgccattgg tatttcgttg ctggtttccc
     4381 tgacgctaac gccaatgatg tgcggctgga tgctaaaagc cagcaagccg cgtgagcaaa
     4441 agcgactgcg tggttttggc cgcatgttgg tagccctgca acaaggctac ggcaagtcgc
     4501 tgaaatgggt gctcaatcat acccgtctgg tgggcgtggt gctgcttggc accattgcgc
     4561 tgaatatctc gatcccgaaa accttcttcc cggagcagga caccggcgta ctgatgggtg
     4621 ggatacaggc ggatcagagt atttcgtttc aggcgatgcg cggcaaattg caggatttca
     4681 tgaaaattat ccgtgacgat ccggcagtgg ataatgtcac cggctttacc ggcggttcac
     4741 gagttaacag tgggatgatg tttatcaccc tcaagccacg cgacgaacgc agcgaaacgg
     4801 cgcagcaaat tatcgaccgt ctgcgcgtga agctggcgaa agaaccgggg gcgaatctgt
     4861 tcctgatggc ggtacaagat attcgcgttg gtgggcgtca gtcgaacgcc agctaccagt
     4921 acacgttgtt atccgacgac ctggcggcac tgcgcgaatg ggagccgaaa atccgcaaaa
     4981 aactggcgac gttgccggaa ctggcggacg tgaactccga tcagcaggat aacggcgcgg
     5041 agatgaatct ggtttacgac cgcgacacca tggcacggct gggaatcgac gtacaggccg
     5101 ccaacagtct gttaaataac gccttcggtc agcggcaaat ctcgaccatt taccagccga
     5161 tgaaccagta taaagtggtg atggaagtgg atccgcgcta tacccaggac atcagtgcgc
     5221 tggaaaaaat gttcgttatc aataacgaag gcaaagcgat cccgctgtcg tatttcgcta
     5281 aatggcaacc ggcgaatgcc ccactatcgg tgaatcatca gggattatcg gcggcctcga
     5341 ccatctcgtt caacctgccg accggaaaat cgctctcgga cgccagtgcg gcgatcgatc
     5401 gcgcaatgac ccagcttggt gtgccttcga cggtgcgcgg cagttttgcc ggcacggcgc
     5461 aggttttcca ggagacgatg aactcgcagg tgatcctgat tatcgccgcc atcgccacgg
     5521 tgtatatcgt gctgggtatc ctttacgaga gttacgtaca tccgctgacg attctctcca
     5581 ccctgccctc ggcgggcgtt ggagcgctgt tggcgctgga gctgttcaat gccccgttca
     5641 gcctaatcgc cctgataggg atcatgctat taatcggcat cgtgaagaaa aacgccatta
     5701 tgatggtcga ttttgcgctt gaagcccaac ggcacggtaa cctgacgccg caggaagcta
     5761 ttttccaggc ctgtctgctg cgttttcgcc cgattatgat gaccaccctg gcggcgctgt
     5821 ttggcgcgct gccgctggta ttgtcgggcg gtgacggctc ggagctgcgg caacccctga
     5881 ggatcaccat tgtcggcggt ctggtgatga gccagctcct gacgctgtat accacgccgg
     5941 tggtgtatct ctttttcgac cgtctgcggc tgcgtttttc gcgtaaacct aaacaaacgg
     6001 taaccgagta aatgacagat cttcccgaca gcacccgctg gcaattatgg attgtggctt
     6061 tcggcttctt tatgcagtcg ctggacacca ccatcgtaaa caccgccctt ccctcaatgg
     6121 cgcaaagcct cggggaaagt ccgttgcata tgcacatggt cattgtctct tatgtgctga
     6181 ccgtggcggt gatgctgccc gccagcggct ggctggcgga caaagtcggc gtgcgcaata
     6241 ttttctttac cgccatcgtg ctgtttactc tcggttcact gttttgcgcg ctttccggca
     6301 cgctgaacga actgttgctg gcacgcgcgt tacagggcgt tggcggtgcg atgatggtgc
     6361 cggtcggcag gttgacggtg atgaaaatcg taccgcgcga gcaatatatg gcggcgatga
     6421 cctttgtcac gttacccggt caggtcggtc cgctgctcgg tccggcgctc ggcggtctgc
     6481 tggtagaata cgcgtcgtgg cactggatct ttttgatcaa cattccggtg gggattatcg
     6541 gtgcgatcgc cacattgatg ttaatgccga actacaccat gcagacgcgg cgctttgatc
     6601 tttccggctt tttattgctg gcggttggca tggcggtatt aaccctggcg ctggacggca
     6661 gtaaaggtac aggtttatcg ccgctggcga ttgctggcct ggtcgcagtt ggcgtggtgg
     6721 cactggtgct ttatctgctg cacgccagaa ataacaaccg cgccctgttc agtctgaaac
     6781 tgttccgcac tcgtaccttt tcgctgggcc tggcggggag ctttgccgga cgtattggca
     6841 gtggcatgtt gccctttatg acaccggttt tcctgcaaat tggcctcggt ttctcgccgt
     6901 ttcatgccgg actgatgatg atcccgatgg tgcttggcag catgggaatg aagcgaattg
     6961 tggtacaggt ggtgaatcgc tttggttatc gtcgggtact ggtagcgacc acgctgggtc
     7021 tgtcgctggt caccctgttg tttatgacta ccgctctgtt gggctggtac tacgttttgc
     7081 cgttcgtcct gtttttacaa gggatggtca actcgacccg tttctcctcc atgaacaccc
     7141 tgacgctgaa agatctcccg gacaatctgg cgagcagcgg aaacagcctg ctgtcgatga
     7201 ttatgcaatt gtcgatgagt atcggcgtca ccatcgccgg gctgttgctg gggctttttg
     7261 gttcgcagca tgtcagcgtc gacagcggca ccacacaaac cgtctttatg tacacctggc
     7321 ttagcatggc gtttatcatc gcccttccgg cgttcatctt tgccagagtg ccgaacgata
     7381 cgcatcaaaa tgtagctatt tcgcggcgaa aaaggagcgc gcaatgaagt tctggcgacc
     7441 cggtattacc ggcaaactgt ttctggcgat tttcgccacc tgcattgtct tgctgatcag
     7501 tatgcactgg gcggtgcgta tcagttttga gcgtggcttt attgattaca tcaaacacgg
     7561 caatgaacag cgattacagc tgttaagtga tgcgcttggc gagcagtatg cgcagcatgg
     7621 taactggcgc ttcctgcgca acaacgatcg ctttgtcttt cagatcctgc gttcatttga
     7681 acacgataat tcggaagata aacccggccc gggtatgccg ccgcacggct ggcgtaccca
     7741 gttctgggtg gtcgatcaaa acaacaaagt actggttggt ccgcgagcgc cgattccacc
     7801 tgacggtaca cggcgaccca ttctggtcaa cggtgcggaa gtgggcgcgg tgattgcctc
     7861 ccctgttgag cggctgaccc gcaatactga tatcaatttc gataaacaac agcggcaaac
     7921 cagctggctg atcgtcgccc tggcaacgtt actcgcggcg ctcgccacct ttctgctggc
     7981 gcgcggtttg ctggcaccgg taaaacgact tatcgatggc acgcacaaac tggcggcggg
     8041 cgatttcact acccgcgtaa cgcccaccag tgaagatgaa ctgggcaaac tggcgcaaga
     8101 cttcaaccag ctcgccagca cgctggagaa aaaccagcaa atgcggcgcg attttatggc
     8161 cgatatttct cacgaactgc gtacgccatt agcggtgctg cgcggtgaac tggaagccat
     8221 tcaggatggc gtgcgcaaat tcacgccgga gacggtggcg tctttacagg cggaggtcgg
     8281 tacactgacc aaactggttg acgatcttca tcagttgtcg atgtctgatg aaggcgctct
     8341 cgcctatcaa aaagcaccgg tagatttgat cccactgctg gaagtggcgg gcggcgcatt
     8401 tcgcgaacga ttcgccagcc gtggcctgaa actgcaattt tccctgccag acagtatcac
     8461 cgtatttggc gatcgcgacc gtttaatgca gttattcaat aacttactgg aaaacagcct
     8521 gcgctacact gacagcggcg gcagcctgaa aatctctgcc gagcagcacg acaaaacggt
     8581 gcgcctgacc tttgccgaca gtgcgccagg tgtcagtgac gatcagctac aaaaattgtt
     8641 tgaacgtttt tatcgcaccg aaggttcccg caaccgcgcc agcggcggtt ccgggctggg
     8701 gctggcgatt tgcctgaaca ttgttgaagc ccataatggc cgcattattg ccgcccattc
     8761 gccttttggc ggggtaagca ttacagtaga gttaccgctg gaacgggatt tacagagaga
     8821 agtatgaccg agttaccaat cgacgaaaac acaccgcgta ttttgatcgt ggaagatgaa
     8881 ccgaagctgg ggcagttgct cattgattat ctgcgtgctg cgagctatgc gccgacgctt
     8941 atcagccacg gcgatcaggt actggcgtat gtgcgccaga caccaccgga tctgatcctg
     9001 ttggatctga tgctccctgg caccgatggc ctgacattat gccgggaaat tcgtcgtttt
     9061 tccgatgttc cgatcgtgat ggtgacggca aaaatcgaag agatcgaccg cctgctgggg
     9121 ctggagattg gcgccgatga ctacatctgc aagccgtaca gcccacggga agtggtagcg
     9181 cgtgtcaaaa ccattttgcg ccgttgtaaa ccgcaacgcg aacttcagca acaggatgcc
     9241 gaaagcccgt tgattatcga cgaaggtcgt tttcaggctt catggcgcgg taaaatgctt
     9301 gacctgacgc ctgcggaatt tcgtctgctt aaaacgctct ctcacgaacc aggaaaagtg
     9361 ttctcccgcg agcaattgct caatcatctt tatgacgact accgcgtagt aaccgaccgc
     9421 accattgaca gccacattaa aaatctgcgc cgcaagctgg aatctctcga cgccgaacag
     9481 tcatttatcc gcgccgttta cggcgttggt taccgctggg aagccgacgc ctgccgcatc
     9541 gtttagtttt agcgacatta ctttgttagc cggagatggc gttcagaccc gatccggcaa
     9601 cattattcca cgcatggtca gcaaactgac attatctctc cgccctggca ggcatcagaa
     9661 aatcgctcat actttaatca gtaaacagca cctttagatg ctgttttcga taagcaattt
     9721 caatcaagga gtcattatgg ctggttggtt tgaactcagt aagagcagtg ataatcagtt
     9781 ccggtttgtg ctaaaagcgg gcaatggtga gactatcctc accagcgagc tgtatacctc
     9841 aaaagcctct gcggaaaagg gcatcgcgtc ggtgcgtagc aacagcccgc aagaagagcg
     9901 ctatgagaaa aaaacggcaa gcaacggcaa attctatttc aatctgaaag ccgctaatca
     9961 tcaaattatc ggctccagcc agatgtacgc caccgcgcaa tctcgtgaaa ccggaattgc
    10021 ctccgttaaa gccaatggca caagccagac ggtgaaagac aatacgtaat tacgatgccg
    10081 ggcgtgggtt gttggcgtcc ggcgctttac cagatccccc ctacagcgct acaatgcccg
    10141 cccttaaagt gggggcctcc cctaaccgct tcatcaggtg aagcggatct gacctgtcat
    10201 cagaacgaga gaattatgtt taaaccggaa ctcctttccc cggcgggaac gctgaaaaat
    10261 atgcgttacg ctttcgctta tggcgcagat gctgtttatg cgggccagcc gcgttactcc
    10321 ctacgtgtgc gcaacaacga attcaaccac gaaaatcttc agctcggcat caatgaagcc
    10381 cacgcgctgg ggaaaaagtt ttatgtcgtg gtcaacattg caccacacaa cgccaagctg
    10441 aaaaccttta tccgtgacct gaaaccggtg gtggaaatgg ggccggatgc gctgattatg
    10501 tccgatccag ggctgattat gctggtgcgt gagcacttcc cagaaatgcc gatccacctc
    10561 tcggtgcagg ctaacgccgt gaactgggcg acggtgaaat tctggcagca aatgggactg
    10621 acccgcgtga tcctctctcg cgagctgtcg ctggaagaga ttgaagagat ccataatcag
    10681 gtgccggata tggagatcga gatcttcgtt cacggcgcac tgtgcatggc ctactccggt
    10741 cgctgcctgc tctctggcta tatcaacaag cgcgacccga accagggcac ctgcaccaac
    10801 gcctgccgct gggagtacaa cgtccaggaa gggaaagaag atgacgttgg caacatcgta
    10861 cacaagtacg agccgattcc ggtgcaaaat gttgagccga cgctgggtat cggcgcgcca
    10921 accgacaaag tgtttatgat cgaagaagcc cagcgtccgg gcgagtatat gaccgcgttt
    10981 gaagatgagc acggcactta catcatgaac tcgaaagatc tgcgcgccat cgcccatgta
    11041 gaacgcctga ccaaaatggg cgtgcattcg ctgaaaatcg aaggtcgtac caaatctttc
    11101 tactattgcg cacgcaccgc acaggtttac cgcaaagcta tcgatgacgc cgctgcggga
    11161 aaaccgttcg ataccagcct gctggaaact ctggaaggtc tggcgcatcg tggctatacc
    11221 gaaggtttcc tgcgtcgtca tactcacgac gattatcaga actacgaata cggttattca
    11281 gtttctgacc gccagcagtt tgttggtgag tttaccggtg agcgcaaggg ggacctcgca
    11341 gcggtagcgg tgaaaaataa attctccgtt ggcgacagtc ttgagctgat gacgccgcaa
    11401 ggcaacatta actttaccct tgagcacatg gaaaacgcca aaggcgaagc tatgccggtc
    11461 gcaccaggcg atggttatac tgtgtggctc ccggtcccgc aggatcttga gctaaattac
    11521 gcgctgctaa tgcgtaattt ctccggggaa accacgcgta acccccacgg taagtgatta
    11581 atttcgatta tttttcccgg atggaaaatt cttagaaacc gatcacatac agctgcattt
    11641 attaaggtta tcatccgttt cgctgaaaaa cataatccat aaaatgctag ctgtaccagg
    11701 aaccacctcc ttagcctgtg taatctccct tacacgggct tattttttgt acataagctg
    11761 taaagaagga ttgatccctg ataaccgtct tccattgacc acatcgatag aatccccctt
    11821 catagcacaa tgcctttcac gtaacggtat cgtgctcgcc caggttccgg ctacgcacag
    11881 ccagaacgcg catgtttgac gcttaccaaa aaatattctc actctccaca tttgaatgtc
    11941 agacgagcga cgccatgtaa tcctgcacct tctgtcttca ggtcaactat ctgcattttt
    12001 ttgccctgag taacacagaa atgggctgca tcatttttta ctatattttc tgcaccagat
    12061 attctgcccc tggctaaaga agcttcggct tcggtgtagt attggttatc gagtttacgc
    12121 tgaatattac ttttatatgc aagaccaaat ttaccgatac ttgtttcatc attatgcaca
    12181 gcacaaccag atataagaaa aatactaatt aatgatatag cagctatctt tttcatctca
    12241 ccttccccca ttaaatacca acgacactct ctagtgttta aatataataa tggcatgatt
    12301 attataattg aataggatta taataaatgt tctgtacaac atttcctaca taagtaggaa
    12361 ttacggacat tgaggccctt cagggtaact ccatgggggc tttaatatat tatattgaag
    12421 atgccactgt ttagttgaat attaggtata tgctcttttt tgaaatttat aggtggcagc
    12481 cgttagtatt cgctgtcccc attgcaagct cctggtggta accactgaat cctccatact
    12541 tgaactgact ttttatcctc cgactttcat cctgttctga ctccaccttt tgttttctgc
    12601 tctacactat ctacagacca atcataaagg cacatacgat catggcagaa tttcccgcca
    12661 gcttactgat tcttaatggc aaaagtactg acaatctacc cttgcgcgaa gcaattatgc
    12721 tgttgcgtga ggaaggaatg acgatccatg tgcgggtcac ctgggagaaa ggtgatgccg
    12781 cacgatttgt agaggaggcc cggaagttgg gcgtcgcaac ggtgattgcc ggtggtggcg
    12841 atggcaccat taatgaagtt tctacggcgt tgattcagtg tgagggggat gacatacccg
    12901 cgctgggaat tttgccatta ggaaccgcca atgattttgc caccagtgta gggattcctg
    12961 aggcactgga taaggcgctg aaactggcaa ttgccggtaa tgccattgcg atagatatgg
    13021 cgcaggtcaa caaacaaacc tgttttatta atatggcgac aggcggattt gggacgcgta
    13081 ttaccacaga aacgccggaa aaattaaaag ccgcgctggg tggcgtctct tacatcattc
    13141 atggcttaat gcgcatggat actctgcaac cggaccgttg tgaaatccgc ggtgaaaact
    13201 ttcactggca aggtgacgcc ctggtcattg gtattggtaa cgggcgtcag gccggtggcg
    13261 gtcaacaatt gtgcccgaac gcgttaatta acgatggctt gctgcaactg cgcattttta
    13321 ccggcgatga aattcttccg gctctcgtat caaccttaaa atctgacgaa gataacccga
    13381 atattatcga aggcgcttcg tcgtggtttg atatacaagc cccacacgaa atcactttta
    13441 atcttgatgg cgaaccgttg agtgggcaaa acttccatat tgaaatactt ccggcggcgt
    13501 tgcgttgtcg attaccacca gattgtccat tattgcgtta atcaaactat tctctcttga
    13561 accagggaga atccgtaatg ccggatgcgg tgtaaacacc ttatccggca tacagaacaa
    13621 tactacaccg taattaattt tacccgtgtc ttctgcaatg ccaatttatc cacctctgac
    13681 acagcatcat cggtaacaat aatgtcaatt ttttcgattg gcagtacctg attaaaaccg
    13741 cgacgattaa atttcgatga atcgagaacg gcaatcactt tatttgcggc ggtgaccatc
    13801 gccccgctaa tcgaataacc ttcattaaag gtcgtaatac cattaaccgc atcaatacca
    13861 tcagcaccga caaacattaa atcggcatta atatcctgca atgaacgctc ggcaatggaa
    13921 ccatgcatcg agcgcgtttt atggcggacc gtgccaccac agacgaccag agtaatgtct
    13981 ttattttcgg aaagggcaaa cgctgccggg agactgttgg taatcaccgt gatattttta
    14041 gcggtcatta atccttcggc gataagcatt gtggtacttc cgctgtcgag aataacggtc
    14101 atcccttcat ggatcatttt gactgccgcc tgcgctattc tgttttttgg cgcgctggcg
    14161 agctgaaatc gctctttaaa cccgacttcc tgggtctcgg tttcactatt accggacatt
    14221 attttcgccg caccgccatg aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa
    14281 tcggcacgga ttgtcgcttc cgaggcagca aatactcccg ccagatcctg aacaagcacg
    14341 gttccctgtt cattcactaa ttggatgatc ttattccttc gctcgaatga attcatcgtc
    14401 atttacccat attcaattgt ggctagtgta aacgaagggg gaccattggt gaatgatctg
    14461 ttttgcacaa taccgggcca gcatcacgct ggcccgcggt ttcatggaat gagcaacact
    14521 ttgcccggca tggcattacg agcgatgtca cgcaccgcct gggtgaagct ttcaaagctt
    14581 ccacggtgag cgattaatgg atccaggctt aacttacgtt ctgtcagcaa ccggctcgcc
    14641 gtttcccact cctgccccgg ccaagggctg gaatagttca tccaactgcc gataaccgtc
    14701 agctctttac gcaatatttt gccaaacgtt gccgatgtta aatgcagatc ctgatgcaac
    14761 gtgcccacca gcgccagttg ggcatgagga ccggcaatct ctaccgccag ttcgacagtt
    14821 tgcggtacgc cagccgtctc gaggataagc tgattaaagc gcagttcgcg taaaacgctc
    14881 tgcatttgcg gcgcgctcat ttcgctactg ttaaatgttt gcatcgcacc gaaagatttt
    14941 gccagtgcca gtttttctga gctaatgtcg atcgccgtca cactctttgc tcccagcgcg
    15001 acagcgcact gaatagccag caggccaatg gttccggcac caataataat aacgttttta
    15061 ttctcacaac cttgcgctaa atgaaaagca tgcaggccaa cggtaatcgg ctcaataaaa
    15121 gccccatcct caataggcat atccgtgggt agagcaaaga cattttttcg cttaacgaca
    15181 atatattcag caaatccacc atcacgccgc gagccaataa aatcatattt tgcgcactgg
    15241 gaataaaacc ctttcaaaca ctctggacaa gtaaaacagg gtaataacgg cacacaggca
    15301 accgcatcgc cagggtgtaa atcatcaaca ccggatccca ccgcatcaat atagccgcta
    15361 aattcatggc ctaacgttat tggataataa tgtgcaccat ttttaaatat cctgggtaaa
    15421 tcggaaccac ataagcccga gctggcaatt tttacccgca cctcatcctg atgtttaatt
    15481 tcaggaatga cgctttttgc aacgcgcacg ataccatcag tatcattcac tactgatttc
    15541 ataaaaactc ctgattgtta agggggataa cccccccctc aggtaaaaat tattctgcga
    15601 gaacggcttt ctcttgttta gtaaaaccac gcgctctacg ccaggtcatg aaaataccgg
    15661 tcagataa
//
LOCUS       DAJVQH010000094        15387 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJVQH010000094 DAJVQH010000000
VERSION     DAJVQH010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.094"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..578
                     /locus_tag="OQM87_002949"
     CDS             <1..578
                     /locus_tag="OQM87_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCS1509015.1"
                     /translation="GSVFGVKDSHDDVVMSGAFAASLRAWSDRKALPALLWQHRMDEP
                     IGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHAHMKAGSLTGLSIGYVLKDWEYDRTK
                     EAFLLKEIDLWEVSLVTFPSNDEARISDVKNALARGEIPEQKKIERVLRDVGLSRTQA
                     KAFMAGGYIALSLRDAEDVGSALNALKNLNF"
     gene            593..1798
                     /locus_tag="OQM87_002950"
     CDS             593..1798
                     /locus_tag="OQM87_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCS1509016.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            1848..2048
                     /locus_tag="OQM87_002951"
     CDS             1848..2048
                     /locus_tag="OQM87_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509017.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            2051..2374
                     /locus_tag="OQM87_002952"
     CDS             2051..2374
                     /locus_tag="OQM87_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCS1509018.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            2371..2781
                     /locus_tag="OQM87_002953"
     CDS             2371..2781
                     /locus_tag="OQM87_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCS1509019.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRLRVDMPADNFGVEPQYL
                     ITFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECGQSHGGNNDDFLFAR"
     gene            2756..3262
                     /locus_tag="OQM87_002954"
     CDS             2756..3262
                     /locus_tag="OQM87_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509020.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            3259..3819
                     /locus_tag="OQM87_002955"
     CDS             3259..3819
                     /locus_tag="OQM87_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509021.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLEPGNGPDGDIEHHTEITLPS"
     gene            3828..3995
                     /locus_tag="OQM87_002956"
     CDS             3828..3995
                     /locus_tag="OQM87_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCS1509022.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNEKVNTDDEL"
     gene            3982..5475
                     /locus_tag="OQM87_002957"
     CDS             3982..5475
                     /locus_tag="OQM87_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCS1509023.1"
                     /translation="MTSFNTIPSNTLVPIFYAEMDNSAANTAQDSGASLLIGHANNGA
                     EIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATVT
                     LTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIQDAINAVPALPFTASSSAG
                     VVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAAM
                     ADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAGD
                     QFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKGK
                     RFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAYV
                     LRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYEL
                     FKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            5475..5831
                     /locus_tag="OQM87_002958"
     CDS             5475..5831
                     /locus_tag="OQM87_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCS1509024.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPASKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            5831..6100
                     /locus_tag="OQM87_002959"
     CDS             5831..6100
                     /locus_tag="OQM87_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCS1509025.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            6242..8077
                     /locus_tag="OQM87_002960"
     CDS             6242..8077
                     /locus_tag="OQM87_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1509026.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGSAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETISPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYIPYPAVDLAQSNNASDATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            8138..9466
                     /locus_tag="OQM87_002961"
     CDS             8138..9466
                     /locus_tag="OQM87_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCS1509027.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEENAGTGRRVETHEYPNRDKPYTE
                     DLGKVTFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKGEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDVARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNAAVEQATTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            9463..10542
                     /locus_tag="OQM87_002962"
     CDS             9463..10542
                     /locus_tag="OQM87_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCS1509028.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEISRQWPGD
                     GGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRELVQIAQALAAPFGIEVVNNGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEDPGARKKKKARVQEDPF"
     gene            10542..11090
                     /locus_tag="OQM87_002963"
     CDS             10542..11090
                     /locus_tag="OQM87_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCS1509029.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCLTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            11090..11515
                     /locus_tag="OQM87_002964"
     CDS             11090..11515
                     /locus_tag="OQM87_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCS1509030.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYASRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            11502..12560
                     /locus_tag="OQM87_002965"
     CDS             11502..12560
                     /locus_tag="OQM87_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCS1509031.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDEFWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRLPIACSTAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWAY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGADGEYSHQLLAPVD
                     NISIAKNELAVLGTISWT"
     gene            12551..13135
                     /locus_tag="OQM87_002966"
     CDS             12551..13135
                     /locus_tag="OQM87_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCS1509032.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSSSDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGIQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRQDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNSTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            13139..13789
                     /locus_tag="OQM87_002967"
     CDS             13139..13789
                     /locus_tag="OQM87_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509033.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            13761..14195
                     /locus_tag="OQM87_002968"
     CDS             13761..14195
                     /locus_tag="OQM87_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HCS1509034.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYLTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(14968..>15387)
                     /locus_tag="OQM87_002969"
     CDS             complement(14968..>15387)
                     /locus_tag="OQM87_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1509035.1"
                     /translation="FGFIVFSITAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAI
                     NTIITTVGLIIFNIFIAKIICGNDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSV
                     TIPNPICAIIYTCIYVYVSDKIGHWLSRTVGGPFLIK"
BASE COUNT         3696 a         3743 c         4499 g         3449 t
ORIGIN      
        1 atggctccgt ctttggtgta aaggacagcc acgatgatgt ggtgatgtcc ggggcatttg
       61 ccgcttccct gcgggcgtgg agtgacagaa aagcgttacc tgcgctgctc tggcagcacc
      121 gcatggatga gcccatcggt gtttacaccg aaatgaagga agacgatgtc gggctttacg
      181 tcagggggcg gttgctcatt gatgatgatc ccctggcaaa acgcgcacat gcacacatga
      241 aggccggttc gttaaccggc ctttctattg ggtacgtcct gaaggactgg gaatacgacc
      301 ggacgaaaga agcctttctg ctgaaagaaa tcgacctctg ggaagtcagt ctggtgacgt
      361 ttccgtctaa cgacgaggcg cggatcagcg acgtcaagaa cgcgctggcc cgcggggaaa
      421 tccccgaaca gaaaaaaatc gaaagagtcc tgcgtgatgt cggactctcc cgtacccaag
      481 ccaaagcatt catggccggg ggctatatcg cactgtccct gcgcgacgct gaggatgtgg
      541 gctctgcact gaatgcactg aaaaatctga acttctaatc aggagaaata cgatggcggt
      601 agatattaaa gatgtcgaac aggtcgcgca ggagctgcag cagaagtttg acgacttcaa
      661 agcaaagaac gacaagcgcg tggatgcgat tgagcaggaa aaaggcaagc ttgccgggca
      721 ggtggaaacc ctgaacggga aactcagcga gctggaaaat ctcaaaagcg atcttgaaaa
      781 agagctgctt gagctgaaac gtccggcagg tggcgcgcaa aataaactgg ccaccgagca
      841 taaagaagcg tttgtgggct ttttgcgtaa aggccgtgaa gatggtctgc gcgatctgga
      901 gcgtaaggca ttgcaggtgg gcaccgatga agacggtggc tatgccgtgc cggaagcgct
      961 ggatcgcaac attctcaccc tgctgaaaga tgaagtggtg atgcgccagg aagccacggt
     1021 gatcaccgtt ggcggttccg actacaaaaa actggtgaat ctgggcggca cggcttccgg
     1081 atgggtgggg gaaacggata cgcgatccca gactgccacc tccagactgg agctgattga
     1141 acctctcatg ggggaaatct acggcaaccc gcaggccacc cagaaaatgc tggacgatgc
     1201 cttcttcaac gtggaggcct ggatcaacag cgagctggca accgaatttg ccgaacagga
     1261 agaaattgcc tttaccaccg gcgatggtac caagaagccg aaagggttcc tggcgtatga
     1321 atccactgat gaaaccgata aggtccgggc gttcggcaaa cttcagcata ttgtatccgg
     1381 cgaagcgacg gcggtgaccg cagacgccat tatcaaactg atttacacgc tgcgtaaggc
     1441 acaccgcacc ggcgcgaagt tcatgatgaa caacaacagc ctgtttgcca tccgtctgct
     1501 taaagacagc gagggtaact atctgtggcg tccggggctg gaactggggc agccgtcctc
     1561 tctggcgggt tacggtatcg ctgaaaacga acagatgccg gatatcgccg ctgatgcgaa
     1621 agccattgca tttggtaact tcaaacgggg ttacaccatc gttgaccgta tcggcacccg
     1681 cattctgcgt gacccgtaca ccaataaacc gtttgtcggt ttttatacca ccaagcgcac
     1741 cggcgggatg ctggtcgatt cgcaggccat caaactgctg aagattgcag cggcgtaatc
     1801 actcaggggc gcggaaccgc gccccctgtt ctgacgggtg aagaatcatg atcctgaaac
     1861 aagatctgaa atggtcaccg gacggtatgc gtgttgaggt cattcgggcc ggtgagtatg
     1921 acgacggggc gcttcctgcc cgggtgcagg agattgcact tcaggccggg ttagcagagc
     1981 gcggaaccag tgcaaaaagc agtaaagcga caaaagagaa aaaagccacg accagtaaag
     2041 agggctgagt atgcttctga caatggaaga gattaaagcc caactccggc tggatgagga
     2101 tttcgatgct gatgaccgcc atctgcaact gctggcctgt gcggcacaaa agcggacgga
     2161 aacgtatctg aaccggaagc tctatgcacc ggatgaaacc attccggaca gcgatccgga
     2221 cgggctgcac ctgccggatg atattcgtct ggggatgctg atgcttatca gccattttta
     2281 cgaaaaccgc tcgtcggtta cggaagtgga gaaacttgac atgccgcaga gttttggctg
     2341 gcttgtcggc ccgtacaggt actttccgca atgaaaattc gtcaggcgca gaccagcgca
     2401 acctacattc tgccggaccc cggtgaactg aataaacgcg tcctgatccg cctgcgggtg
     2461 gatatgcccg cggataactt tggcgtggag cctcaatacc tgattacgtt ccggacatgg
     2521 gcgaaggtta tccagaccag tgccaccacc tggcaggaaa ccgcgcagac cggggacgcc
     2581 atcacccatt acatcaccat tcgttaccgc cgggggatta ccgctgatta tgaggtggtc
     2641 tgcggtgaca gtgtgtaccg ggtgaaacgt cagcgcgatc tgaacggggc gcggcgcttt
     2701 ctgctgctgg agtgtacgga actgggcgaa tgtgggcaga gtcacggagg caacaatgac
     2761 gacttccttt ttgcacgttg attttcagca gcccgcggag atgcgcttta accgcgcccg
     2821 tgtccggcgg gcgtttgtca cgattggtca gcgtcatatg cgtgatgccc gtcggctggt
     2881 gatgcgccgt gcgcggtcgg caccgggtga aaaccccggt tatcagaccg gacgcctggc
     2941 tcgttcgatt ggttacatgg tacccagagc cagtaaacat cgccctggtt ttatggcacg
     3001 tatagcccct aaccagcgta atggagaggg aaaccgccgt atcaccggtg atttttatcc
     3061 ggcttttttg ttctatggcg tgaggcgagg ggcaaagcgt cgtcgcagcc atcatcgtgg
     3121 tgcatccggt ggcagcggct ggcgactggc tccacgtaat aactttatgg tggaaacgct
     3181 tgaaaagaac cgcagctgga cacgctattt tctggcgcgg gaattgcgta aatcactgaa
     3241 gccggagcga cgacacagat gaaactgacg cctgttattg ctgcgctgcg tgcccgctgc
     3301 ccgtattttg aaaaccgggt ggcaggcgcg gcacagttca aaaatctgcc ggaggtcgga
     3361 aagctgagac tcccggcggc gtatgtggta ccgggtgatg actctccggg agaaaacaaa
     3421 agccagaccg actactggca ggagctgaaa gagggcttct ccgtggttgt catactgagt
     3481 aacgggcgtg atgagcgcgg tcagtttgcc tcgtatgatg tggtggacga tgtccggcaa
     3541 atgctcttta aggccctgct gggctggaac ccggaagcgt gcggtaaccc gattacctat
     3601 gacggcggca cgctgctgga tctgaatcgt catgagctga tttatcagtt cgatttttcg
     3661 gtcatcagcg agttgaccga agacgatacc cgccagcagg atgacctgaa cagtctggat
     3721 gaactgcgaa cgctggcgat tgatgttgat tatctagagc ccggtaacgg gcctgacggc
     3781 gatatcgaac atcacaccga aataaccctt ccttcctgag aatcttcatg tttgtgaaac
     3841 ctgttaaagg gcggtcagtt cctgaccctg cccgcggaga ccttttgccc gccgaggggc
     3901 gaaatgttga tgagaacaac tactggctgc gccgtgaagc agcgggtgat atccggcgcg
     3961 tgaatgaaaa ggtgaatacc gatgacgagc tttaacacca ttccgtcaaa tacgctggtt
     4021 ccgatttttt atgcggaaat ggataactcg gcggcgaata ctgcacagga cagcggagca
     4081 tcgctgctga ttggtcatgc caataacggt gcagagattg ttgccaacag tctggtgctg
     4141 atgccatcgg cagactatgc acgccagatt tgtggtgcgg gaagtcagct ggcgcgtatg
     4201 gtcgaggctt atcgccagac cgacccgttt ggtgagctgt atgtgattgc cgttccagaa
     4261 gccacaggcg cggcggcaac ggttacgctg acggtgaccg gggaagcaac cgaaagcggc
     4321 acggtgaatg tctatgtggg acgtacccgc gtgcaggctc cggtgaccaa cggcgataac
     4381 gtcacgacga ttgccagcag tatccaggat gccatcaatg ccgttccggc cctgccgttt
     4441 acggcttcat cttcggcagg cgtggtcaca ctgaccgcgc gtcataaggg gctttgcggg
     4501 aatgaaattc ctgtcagcct caattactac ggctttggtg ggggcgaagt gctgccagcg
     4561 ggcgtacaga ttgccgtggc gacgggtacc gccggaacgg gtgctccggt tctcaccggc
     4621 gcggtggctg caatggcgga tgagccgttt gattatatcg gcctgccgtt caacgacacg
     4681 gcctccgtta acacgctggt gaccgagatg aacgatacca gcggtcgctg gagctatgcg
     4741 cgtcagctgt atggtcatgt gtatacggca aagatcggca cgctgtcaga actggtgacc
     4801 gcaggtgacc agtttaacca gcagcacatt accctggcgg gatacgaaaa agagacccag
     4861 acgcctgccg acgagctggc ggcaagccgt accgcccgcg cagcggtgtt tattcgcaac
     4921 gatccggcac gtcccacgca gaccggtgag ctggtgggta tgctgcctgc gccgaagggg
     4981 aaacggttca cgatgaccga acaacagacc ctgctgtctc atggcgtggc aacggcgtat
     5041 gtcgaaagcg gggtgctgcg cattcagcgt gatgtcacca cgtacaggaa aaacgcttac
     5101 ggtgttgcgg ataacagcta cctcgacagc gagacgctgc ataccagcgc gtatgtgctg
     5161 cgcaaactga aatccgtcat taccagtaag tacgggcgtc acaagcttgc cagcgacggt
     5221 acccgctttg gtcccggtca ggcgattgtc acaccggcgg tgatcaaagg ggaactgctg
     5281 gcaacctacc gtcagcttga gcgtgcgggg atcgtggaaa actacgaact gtttaagcag
     5341 tacctggttg tggagcgtga tgccagcgat ccgaaccgcc tgaacacgct gttcccgcct
     5401 gactatgtta accagttgcg tgtctttgcc gtggttaacc agttccgtct tcagtattca
     5461 gaggagtctg cataatggcc cgtatcgggg gaacctgtta tttcaaaatt gacggtcaac
     5521 agctatcgct gaccggcggc attgaggtgc ccatgaacag gacggtcaat gatgacatca
     5581 tcggcctgga cggttcagtg gaccgcaagg aaactcaccg tgcgccttat gtcaaaggga
     5641 ccttcaaggt gccgaagaat tttccggcga gcaaaatcac ctcgtctgat gagatgacca
     5701 tcactgccga gctggcgaac ggtcaggtct atgtactgtc gtctgcctgg ctgcacggcg
     5761 aagcgaacca taatgccgaa gaaggcacgg tcgatcttga gttccacggt gaagaagggg
     5821 attaccagta atgaaagagc ttgagttaaa gaaaccgatt accgctcatg gcgagacact
     5881 ctccgtactg gagtttgatg agcccaccgg gaaagatgtc cgcgagctgg ggtatcccta
     5941 ccagatgaat caggatgagt ccgtcagact tctggcgcat gtggtatcga aatatattgt
     6001 gcggctggcg aaagtgccgc aaagctctgt cgaccagatg tctccggcag acctgaatgc
     6061 agcggcgtgg cttgtggctg gttttttcct ccaggcctga cggctgaata ccttactgat
     6121 cgcttctttg attgcgccag ctactggcgc attaatccct tcgaattgct gaatatgccg
     6181 atcagtgaaa ttcccttgct ggtcagtcag gcaaacagga tagagcagga gaaacgcaca
     6241 catggctgaa tttgagctta aggcgttgat caccggtgtc gacaggcttt ctcccgcgct
     6301 gtcgaaaatg caaaagaaaa tccggggatt taaacgccag gcggaagaag cgtcacaggg
     6361 tgggctggcg cttggtggcg gactggcagc gggtctgacg ctttccctga aatcttatgc
     6421 cgatcaggaa aacgccgcca ccgggctgaa agtcgccatg atggatgcga atggcgaggt
     6481 tggaaagagc tttcaggaca tcaataaact ggctattggc ctgggtaacc agctacccgg
     6541 tacaacggct gatttccaga acatgatgca gatgctggtg cgtcagggga tcccggcaga
     6601 aaatattctt ggcggtgtgg gtaaagcgac agcttatctt gcggtacaac tgaaaaaaac
     6661 accggaagcg gctgctgagt ttgctgcaaa gatgcaggat gctaccggaa cggcgtcaga
     6721 agacatgatg gggctgttcg acactatcca gaaggcgttt tatctgggcg ttgacgatac
     6781 caacatgttg tccttcttca ctaaaaccag ttctgttctg aagatggtga acaaggacgg
     6841 tcttcaggct gcacagagcc ttgcccccat cagcgtcatg atggatcaga tggggatgaa
     6901 cggggagtcg gcaggtaatg ccctgcgaaa agttatccag tccggattaa gcgttaagaa
     6961 aatcagggac gtcaataaaa tcatggcccg ccagaaactc ggggtacagc tcgattttac
     7021 tgacggcaaa ggaagttttg gcggtcttga taacatgttc aggcaactgg caaagctgcg
     7081 aaaactgacc gacgttaagc gaacaggtgt acttaaggca atatttggtg atgatgccga
     7141 aacccttcag gtggtcaatg cactaatcga taaaggaaag gatggctacg atcagatcca
     7201 gcagaagatg aataaacagg ccagcctgaa taaacgtgtt caggcccagc ttggtacgct
     7261 gtccaacctg tgggaggcaa tgacggggac cgcaactaac ggccttgcgg ctattggcgg
     7321 cgcattttct ggtgacgcca aaaatatcac gcaatggctg ggggagttgg gggagaaatt
     7381 cacgaagttt gcggatgaaa atccccgggt tattcgcggc gtcgtcgggc ttgctgccgg
     7441 tcttgcgatt ctgaaactgg gattgatggg cgtgggcagt gccatcagta tcgtcagcag
     7501 gatcatgtcg atgacgccga ttggcatgat tgcgacggcg attgccctgg ctgcgggatt
     7561 aattatcact aactgggatg ttgtcggacc ttattttaag aaactctggg aaaccattag
     7621 tccttatttt gaggctggct gggaacttct gaagaaggtt tttgcctggt cgccgctggg
     7681 gatggtgatc aataactggg gaccggttgt taagtggttt caggatatgt gggataagct
     7741 gaagccaatt attgagtggt ttaccgacag ttcaggtgac acggtcgatg ccattaactc
     7801 ggcgcagtgg ggcgcgggtg cttatgatgc ttatgggacg ggaataccgg cgcggggata
     7861 cataccttat ccggcggtgg atctggctca gtcaaacaac gcctccgatg ccacaggccc
     7921 gaatcccttc atgattaaca aagcttctgc gccaaaagtt gatggtgaga tcaaggtctc
     7981 ttttgtgaat tcgcctccgg gtatgcgggt tatggaaacg cgatccagcg gttttgatgt
     8041 cagccatgat gttggctata cgcgctttgg caggtaatga aaaattaatc tgttaatgag
     8101 tcccactccg gtgggatttt ttatgtacgg agtttatatg acgtggaaag acagacttca
     8161 ggacgcgtca tttcgcggtg tgccgtttaa ggttgaagaa gaaaatgcgg gaaccggtcg
     8221 tcgtgtggaa acgcacgaat acccgaaccg cgacaaaccc tataccgaag atctgggaaa
     8281 agtcactttc cgcccgtcca tcacggctta tgtggtggga gatgactgct ttgaccagcg
     8341 cgatcgcctg attgacgcgc tgaataaacc cggtcccggc acgcttgtcc atccgacata
     8401 cggtgagctg aaagtctgtg ttgacgggga ggttcgggtc agcacatcga agggtgaagg
     8461 gcgtattgtc cgctttgacc tgaagtttgt cgaagcggga gaactctctt accccacatc
     8521 aggtgcggcg acggcgcaga cgctgatgtc atcctgttct gcactggatg actgcatcag
     8581 tgacagtttc agtggtttca gtatcgatgg cgtggcagat tttgtgcaga acgacgtcgt
     8641 cggtaatgcc agcacaatgc ttgggtatgt ttctgatgcg atgaaagtgg tggattctgc
     8701 cgtatcggat gtcgccaggc tgttgcaggg ggatatctcg gtacttctgc cgccgccatc
     8761 gtcaggcaaa aatttcgttg agcaggtgca gaaaatgtgg cgtaccggga aacgccttta
     8821 tggtaacgcc agcgacctgg tcaccatgat caaaacgctt tccggtgtca gcctcggcag
     8881 cgatctgcaa ccgcgcggcg tctggaaaac ggacagtaaa accaccgcca cggcgacgca
     8941 gcagcgtaac gtggttgcca gcacccttcg tacgaccgca atcagcgaag cggcgtatgc
     9001 cgtcacccga ttgcctgcgc caacaacttc cgcggtgatg cagaatgccg cagtggagca
     9061 ggcaacaaca cctgcgcaga gcactggctg gccttccgtc acgcatccgg cactgaacaa
     9121 tgcaccggcg gtgaaaaaca cggttgacct gccgacgtgg gaagaactga ctgacattcg
     9181 cgacacactg aatacggcaa ttgataagga gttgtcccgt acaaccagtg atgcgctgtt
     9241 tctggcgctg cgccgggtga aagcagatct gaatgcggat atcaacacgc gccttgaaca
     9301 gtctgcacgg atcattcagc gcacaccgga tgaggtttta cccgcgctgg tgctggcggc
     9361 gacctggttt gataacgcgg cgcgtgacgc ggacattatc cggcgtaatg ccattacgca
     9421 tcccggcttt gtgccggtga tccctctgaa ggtgccagtg caatgaacga taacgtcacg
     9481 ctacgggtaa atggccggga gtggaatggc tggacatcgg tgcgcatcgg tgccggtatt
     9541 gaacggctgg cgcgggattt cagtgtggag atctcccgcc agtggccggg agatgggggt
     9601 atcaccacgc ttcagccgcg cattaaaaac ggttcaaaag tggaagtgct gattggtgat
     9661 gagctggtga tcaccggctg ggtggaggcg acgcccgttc gttacgatgc ccgttcggtc
     9721 agcaccggta ttgccggacg cagtctgacg gctgacctga ttgactgtgc agccgaaccg
     9781 acacagttta acggacgaga gctggtacag attgcgcagg cgcttgctgc gcctttcggc
     9841 attgaggtgg tgaacaacgg tgcgccgtcg ggtgttattc ctgacgtcca gcccgatcac
     9901 ggtgaaacgg tgattgaggt aatcaacaaa atactcggtc agcagcaggc actggcttac
     9961 gacgacccgc acggcaggct ggtgattggt ggtattggct caacgcgggc acatactgcg
    10021 ctggtactcg gggaaaacat cctttcctgc gatacggaga agagtatccg ggagcggttt
    10081 tctgtttacc aggtggcggg gcagcgtgcc ggaaacgacg atgatttcgg tgaggccacc
    10141 accaccgcgc tgcgggcccg cacagaggac gcatttattg cccgttaccg tccgatgtat
    10201 atcaggcaga cagggcaggc cacgggggca ggctgtattg cgcgtgctga ctttgaagcc
    10261 cggcaacggg cggcgcggac ggatgaaacc acctatgtgg tgcagggctg gcgacagggt
    10321 aacggtacgc tgtggcagcc caaccagcgg gtgattgtct tcgatccggt ctgtggtttt
    10381 gacaataccg aactgcttgt ctcggaagtc acgtttactc aggaccagaa tggcactctg
    10441 acggaaatcc gtgtcggccc gcctgatgct tatctgcctg aacccgaaga ccccggcgcg
    10501 cggaaaaaga aaaaagccag agtacaggag gacccgttct gatgaggacg attgaagcca
    10561 tgcagcgaca actcctcggc ctgattgggc gggccgtggt gaaaagcatc agtgccgcca
    10621 cgaaatgtct gaccgtggat gtgtccctga ttgccggtga acccaaagcc ggggttgaac
    10681 atcttgaacc ctacggtttt accgcaaggg caaacagcgg tgcggaagcg gtggtgttgt
    10741 ttccggatgg cgaccgttct catgcggtgg ttgttacggt gtcagaccgg cgctaccgcc
    10801 tgaaagggct gcagacgggt gaggtggctg tctatgacga tcaggggcag tccgtgacgc
    10861 tgacccggga ggggatcgtg gtggacggtg caggtaaaac gatcacgttt cgcaatgcac
    10921 ctgaagcacg ttttgaaatg gacctggaag taacaggaca ggtgaaagac ctgtgcgact
    10981 ccggcggcac caccatgtca gcaatgcggc ttgcctataa cggtcatcgt cacagagaga
    11041 acggtcaggg cagtaacacc gataaacctg ataaagctat ggaggcatga tggaactgtg
    11101 gctgacggtg aacggtaaac gcacctgcgc cagcgcaccg ctggatccgc tgacccgcgc
    11161 cgtggtgatt tccctgttta cctggcggcg ggcggagcct gatgacaacg ccgacgtccc
    11221 gatgggatgg tggggggata cctggcctgc ggtacagaat gaccgttacg cctccagact
    11281 gtggctgctt cagcgcagca aactgaccaa tcagctggtg cagacggtaa gggggtatat
    11341 ccgcgaatgc ctgcaatgga tgactgatga cggcgtggtg tcccgtattg atctggatat
    11401 ccgccgcacc gggattaatg aactgggtaa cagtatcact ctctggcgtc gtgacggacc
    11461 ggtaatgatt tcttttgatg atctgtggag tgcgataacg catggcggac agtgaatttc
    11521 agcgcccgac gctggcagaa aatatcagta tgctccgtaa cgatttattc gccaggctgg
    11581 acgtcagcga cacgctccgg cgcatggatg aagacgtgcg ggcaaaggtg tatgcggcgg
    11641 cgctgcatac ggtttacggg tacatcgatt atctggcaat gaacatgctg cctgacctgt
    11701 gcgatgagtt ctggctggcg cgacatgctg cgatgaaacg gtgtccgcgc aagggggcca
    11761 cggctgccag cgggtatatg cgctgggaag gtgtcagcga tggcctgaag gtgaccgccg
    11821 gaagtgttat tcagcgcgat gacctggttc agtacacggc aactgccgat gcaaccagct
    11881 ccggtggtgt cctgcgcctg ccgatcgcct gttcaactgc aggcgcggtc ggtaacgctg
    11941 acgacggtac ggcattaatc ctggtcacgc cggtgaatgg tctgccgtct tccggtgtgg
    12001 cagataccct gacaggtgga tttgatactg aagatctgga aacgtggcgc gcccgcgtca
    12061 ttgagcggta ttactggacg ccgcagggcg gggctgacgg ggactatgtc gtctgggcta
    12121 aagaagtgcc cggcattacc cgcgcatggg cataccgtca ctggatggga acggggactg
    12181 tcggtgtgat gattgccagt agtgacctga ttaatcccat tccggaagaa tcaacggaaa
    12241 cggcggcaag acaacatatc gggccgctgg ccccggtggc aggctctgat ttgtatgtgt
    12301 tcaggccggt ggcgcatacg gtggattttc atatccgtgt gacgccggat acaccggaaa
    12361 tacgggctgc cattaccgcg gagttgcgtt cgttcctgct gcgtgatggt tatccgcagg
    12421 gagaactgaa ggtatcgcgt atcagtgagg cgatttccgg tgcggacggg gaatacagcc
    12481 atcagttgct tgcaccggtg gacaatatct ccattgcgaa aaacgaactg gcggtactgg
    12541 ggacgatttc atggacgtga caaacgatga ttacatccgc ctgttatcgg cactgttgcc
    12601 gcccggtccg gcgtggtcat ctagcgatcc ggcgattgcc ggtgcggcac cgtcattaac
    12661 ccgtgttcat cagcgtgcgg atgccctgat gcgggagctg gatccgcgca ccaccactga
    12721 actgataaac cgctgggagc gtctgtgcgg tctgccggat gaatgtattc ccgcaggaat
    12781 acagaccctt cgccagcgtc agcaacgact ggatgcgaag gttaacctgg cgggcggcat
    12841 caatgaggat ttttaccttg cacagcttgc tgccctgggc agacaagacg ccaccatcac
    12901 gcgatacgac aaaagcacgt tcacctgctc atcggcctgt actgacgcgg tgaatgcgcc
    12961 ggaatggcgg tattactggc aggtcaacat gccagccgcc accaacagca cctggatgac
    13021 atgtggcgat ccctgtgatt ccgcactgcg tatctggggc gacaccgtcg ttgaatgtgt
    13081 gcttaacaaa ctctgccctt cgcataccta cgtaattttt aaatatccgg agtaatccat
    13141 gcatcgtata gacacgaaaa ccgcgcagaa ggataagttc ggcgcgggta agaacggttt
    13201 tacccgtggt aacccccaga ccggcacacc tgccaccgat ctggatgatg actactttga
    13261 catgttgcag gaggagcttt gcagcgtggt ggaggcatcc ggtgccagcc tggagaaggg
    13321 gcggcacgat cagttgctta ccgcacttcg cgcgctgctg ttaagccgca agaatccgtt
    13381 tggcgatatc aaatcggatg gcacggtgaa aacggctctc gaaaaccttg gtttgggaga
    13441 agcagctaaa aggaatgtag gtacaggggc gaatcagata cctgatatga gcctgttcgc
    13501 gtcaattaat accgtaacgg ctgctgcgca aaaatttccg tctggattaa ttttacagtg
    13561 cggtcagttg aatggtgccc cgaatgtatc ttcaacatac gggatgaggt tcccgatgac
    13621 gttctcaaga gtcattgctg tcgtagttac attgaacgtt actggcgcgg cagggcagcc
    13681 gactgtatcg gcgacaagtg tccagaacac tggatttaat attacggtgt cgcccggttc
    13741 aggatacggt tcatctgctg atgcgtatta cattgcaatg ggatattaac aaaatgtcat
    13801 atttttattc tgcatcgaca aacggatttt atttgactga atttcacggc accaatattc
    13861 ctgatgatgc agtggaaatc tcggaatcag agtggaaaac actgattaat gcacagagtg
    13921 taacaaaaat gattacctgt ggtgagaacg gtcatcctgt cattgttgac cgtccttctc
    13981 caacaccaga acaattagcc ttaataaatg atgaaaagaa atctgcactg atagcagagg
    14041 caacgaatgt aatagctccg cttcaggatg cggttgattt aggtatggca acagatgatg
    14101 aaacgaaact gttactggca tggaaaaaat atcgggtgct gcttatgcgt gttaatgtag
    14161 taaaacccga gtggcctatg catccaaata aatagacttg ttaatctagg ataattgttg
    14221 gaataatact catttttcag taggttcagg tagcctgggt ataatgctca cttctacaat
    14281 gatttcaact tattttattt aattgatatt acgaaactta actcatacat aaacaatatg
    14341 actcaatact gtcctaaata gctgcgcaga aaagtgctcc actttcgctc agcccttgct
    14401 gggcgaagca tcaggaaggc gttctcagct aggttgtgtt atatctggct tgagttttgt
    14461 tgcccatacg caacacatta cccatctgta aacgacaagt gtgacagaat taggtgcagc
    14521 tatttagact atatcgacgc agtaacgata atagatatcg aaactgtact agatattagc
    14581 tacttatggt taccggggga agtcttttga gcactggtgt tatcaatcgg cttcaaataa
    14641 ttcaccaata aattatattg cgatagctca tcaccttcct ggttgtttat agccagttta
    14701 tcagcgtcac cacctactgt ttatgctttt atttctttga ttaatgagta tatgatattt
    14761 taattcatct ggtgtatttc tttctcatgt gttggccgac tgaatatctg ttcagtgaag
    14821 gggtaatttt atgtttgcac ttcgaacatt cccttcagtt gccaagaaag aaaaccgtaa
    14881 catttgcgca ttaattttaa cagtataatc tgagcataaa tatgcgctat atatctataa
    14941 aaaggcgcaa ttaattgcgc ctttttatca ttttatcaaa aatggcccac cgactgtcct
    15001 cgatagccaa tggccaattt tatctgatac atatacataa atacaggtat atatgattgc
    15061 gcagatagga tttggaatgg tcacgctgaa gcctatgatt gctaaagcca tagggataaa
    15121 taatttggtg atatactcca ttccacatat gttaagagta tttcttccag ctcttacaat
    15181 aaagtcattc ccacaaatta tttttgctat aaaaatatta aatattatca aacctaccgt
    15241 ggtgataata gtattaatag cttgaatata actacttgat attatgtctg cattgaatat
    15301 tgagataatc ttaaaaagca aaggtttctg gttaaggagt tgatacgcac tcattatcgc
    15361 ggtaattgag aagacaatga aaccaaa
//
LOCUS       DAJVQH010000095        15386 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJVQH010000095 DAJVQH010000000
VERSION     DAJVQH010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.095"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..445)
                     /locus_tag="OQM87_002970"
     CDS             complement(<1..445)
                     /locus_tag="OQM87_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCS1509036.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDT"
     gene            complement(432..1454)
                     /locus_tag="OQM87_002971"
     CDS             complement(432..1454)
                     /locus_tag="OQM87_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1509037.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNLVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(1468..2970)
                     /locus_tag="OQM87_002972"
     CDS             complement(1468..2970)
                     /locus_tag="OQM87_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1509038.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDMREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLNTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3110..4067)
                     /gene="ytfQ"
                     /locus_tag="OQM87_002973"
                     /pseudo
     CDS             complement(3110..4067)
                     /gene="ytfQ"
                     /locus_tag="OQM87_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4377..4907
                     /gene="ppa"
                     /locus_tag="OQM87_002974"
     CDS             4377..4907
                     /gene="ppa"
                     /locus_tag="OQM87_002974"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCS1509039.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5287..5628)
                     /locus_tag="OQM87_002975"
     CDS             complement(5287..5628)
                     /locus_tag="OQM87_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCS1509040.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(5631..9410)
                     /gene="tamB"
                     /locus_tag="OQM87_002976"
     CDS             complement(5631..9410)
                     /gene="tamB"
                     /locus_tag="OQM87_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCS1509041.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9407..11140)
                     /gene="tamA"
                     /locus_tag="OQM87_002977"
     CDS             complement(9407..11140)
                     /gene="tamA"
                     /locus_tag="OQM87_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCS1509042.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11346..11984
                     /gene="msrA"
                     /locus_tag="OQM87_002978"
     CDS             11346..11984
                     /gene="msrA"
                     /locus_tag="OQM87_002978"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCS1509043.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12307..13650
                     /locus_tag="OQM87_002979"
     CDS             12307..13650
                     /locus_tag="OQM87_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1509044.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(13712..13918)
                     /locus_tag="OQM87_002980"
     CDS             complement(13712..13918)
                     /locus_tag="OQM87_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCS1509045.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14243..14797
                     /locus_tag="OQM87_002981"
     CDS             14243..14797
                     /locus_tag="OQM87_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1509046.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPRSQFIVDSNGVARGAWQLDDESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(14860..>15386)
                     /locus_tag="OQM87_002982"
     CDS             complement(14860..>15386)
                     /locus_tag="OQM87_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509047.1"
                     /translation="ACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRG
                     KFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLED
                     EDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQ
                     LALPVLVRCKNSET"
BASE COUNT         3644 a         4198 c         3790 g         3754 t
ORIGIN      
        1 gcgtgtcata aatgggatgg tttatcggaa tcgactcttc cgaaacgaga tagctgacgc
       61 cgcgcaaaaa gaacatcccc gcaagcgtaa tgataaatgc cgggatcttc agggcgtcga
      121 tcagaagccc cataaatgcg ccgaaggcac agcccatcac cagcactagc ggaaacgcca
      181 gcagcggcga gaggccgaaa tcgccaacca cttttgccag aaacacgcca gtaaaggcga
      241 tcaccgaacc gacggagaga tcgataccgc cagagaggat cacaaaggtc atgccaacgg
      301 cgattatccc aagaaaggcg ttatcggtca ggatattgca gatcaccctt gtggaagcaa
      361 aaccgggaaa ctgggtcagg cagtagagat agcccagcac aaagacgccg atggtgatca
      421 tcagcggcaa gttacgtttt atcatggcta cgtactcctt taatcagact gataaagcgt
      481 tgcgactgaa caatcagcac acaaagcacc accaccgctt tcaccaccag gttcatctcc
      541 ggcggaaagc ccgaaagcaa aattccggtg ttcatcccct gaataatcag cgcccccact
      601 accgagagca gcaggttaaa gcgcccgccc atcagcgatc cgccgccaat caccaccgcg
      661 agaatggcgt ccagctccag ccataatccg gcgttgttgg catcggcacc gcaaatatcc
      721 gccgccacga taatgcccgc aatcgccgca catagcccgc tcaacacgta ggtgagcatg
      781 acgatgattc gcgtgtttac cccggcattt tttgccgccc gaatgttgat accaacgact
      841 tcgataaaca tccccagcgc cgttttgcgg gtcaacagcc agaacaggag aagcgtcagc
      901 accgcgataa tgaccggcgt tggcaggaac aacagcgatc cgctgccaaa ccatgagaga
      961 tccggcgagt taaacgtgac gatctgcccg gcggtgatca gttgcgccac gccgcgcccg
     1021 gcgaccatca ggatcagggt ggcgacaaac ggctgaattt tgaggatcgc taccagtatg
     1081 ccgttccaca atcccgccag gatgccggtg cccagggcgc ttaacaaaac aatcggcagg
     1141 ctgaatcccg cgaccgtcat cgcagccgtt gtggctccgg cgatagccat caccgccccc
     1201 acagagagat cgataccacc ggtggcgatc accagcgtca taccaatcgc cagtaacgca
     1261 acgggagctg cacggttaag aatgtcgata gggctaccga acaaacgccc atcctgaagc
     1321 accacctgcc agaaatgcgg ggctaccagg ctatcgacca gcagcaccag caacagtgcc
     1381 gccagctgcg gcatccccgt cggccagtga aaacgccttt ttggcggcgt agtgtcaggg
     1441 agagattgag gcatcacact gttctcctta cgccgcaatg gcgttcatga tcgccggaac
     1501 ggaaagctgt gccagcggga tctccgccac ctgtttgcga tcgcgcatga tgatcacccg
     1561 gtcggcatag cccaccagct cttccagttc ggaggagatc accagcagcg ccagaccgtc
     1621 agcacacagc gtttcaatca ggcggatgat ctcggcgtgg gcaccaacat caatgccacg
     1681 ggttagctca tcaaggatca gaaattgcgg tcgggtcagt agccaacgtg aaagcagcac
     1741 tttttgctga ttgccaccgg agagaaattc aatcggttgt tcagttgaag gtgtgcgaat
     1801 gccaagctgg cggataaagc gttcggcaat ctcttgctgc tctttgcggg aaatgggtcg
     1861 tagccagccg cgctgggcct ggagagcaag gatgatattt tcccgcaccg aggcggcggc
     1921 gatgatgcca tcggttttcc tgtcttccgg gcagaatccg atgcccagta ctgaagcctg
     1981 atgtggcgat cgcagggttt gcggtttgcc tttgatcaac gccgtgccgc tgtcagcagg
     2041 tttgataccg aagatcactt cggcggtttc ggtacgtccg gatcccagca agccagccag
     2101 accgacgatc tcgccggggc gcacttcgag atcaaacggt gctatcgttc cttttttgcc
     2161 gtaatttttg aacgcggcaa cgggtttgtc gctcaacaat gttcgcccgg cacgctgtag
     2221 cgcgtgggta tccagctcgc gccccagcat catttttacc agttcgatct gcggtagctc
     2281 gcgcgtttcc cgacagccta cgaaactgcc gttgcgtaag acggtgatcc gatcgctgac
     2341 ctgatagacc tgatcgagaa agtgagtgac aaagatcagg ctgacgccgc gatcgcgcaa
     2401 ctgacgcatc aggccaaata atagctccac ttcttgggtg ttgaggctgg cggtgggttc
     2461 atcgaggatc agcactttgg cggagagatc gatagcccgg caaatggcga cgatttgctg
     2521 catcgccacc gaaaaacggt taagcggttc gcgcatgtcg agggagaaac cgtaagatgc
     2581 catcagttcg gtggcgcgct tttccatctc tttacggcgt agaaggccga aacgtttggg
     2641 ttcgcgacct atgaatagat tatcagcaac cgacatattg ggtagcaggt tgacttcctg
     2701 atagacggtg ccgataccga gttgttgtgc atgggcggta tttttcggtg agatagtctg
     2761 gccttccagc cagatggtgc cgcgatcggc gtggtataca ccagttaatg ctttgattag
     2821 cgttgatttt cccgccccgt tttcaccaag cagcgccatg atttcgccac ggcgcaggct
     2881 gaaatcaacg ttgtctaacg ctttgacgcc ggggaaaaat ttacttaatc cttcggtgcg
     2941 gaggatctcc tggtgttggt cggtcgtcat ggtttccccc ggtgtaaagc gtgctcccag
     3001 taggcatgat aagacgcggt aagcgtcgca tcaggcttca gtgcaccatc gccggatgcg
     3061 gcgcgagcgc cttatccggc ctacgaatcg gcatcaggca tagcaaccct caatacccca
     3121 tatttttctt cttctctaac tcttcttttg cggtatcagg caggtaaagg gtggatttgg
     3181 ttaacgtcag cttttcaggc atggtgccgt cttttttgta tttctccagc gcatcgaaag
     3241 cgggtcccgc catgttcggc gtcagttcaa cactggcgtt cgcttcgcca tccatcatcg
     3301 ctttgtagat gtccggtaca ccgtcaatgg aacccgtgag gatatctttg cccggtttca
     3361 ggcccgcttc tttaattgcc tgaattgcac caatcaccat gtcgtcgtta tgggcgtaaa
     3421 ccatgcagat gtttttgccg ttgttttccg ctttgataaa gctctccatg acttctttgc
     3481 ctttactgcg ggtgaagtca cctgactgcg agcggataat tttgatattt ggcgcattct
     3541 taatggcttc ggcaaagcct ttcttaacgg tcaatggcga cgctggcccc aacggtgccc
     3601 tgcagctcca ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc
     3661 aacttacctt cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc
     3721 acatcgatgg aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat
     3781 accggttccc aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttgtgca
     3841 acgaaggaac gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc
     3901 aacgtaattc cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag
     3961 ccggattccg atctgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac
     4021 gacatggctg ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg
     4081 tcggggtaat tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg
     4141 taacggattg cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt
     4201 aatcgctttc atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat
     4261 accgtcttaa aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg
     4321 cgagttggct ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga
     4381 gcttactcaa cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga
     4441 tcccggctaa cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg
     4501 ttgaccgctt catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca
     4561 ccctgtctct ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc
     4621 ctggttctgt gatccgttgc cgtccggttg gcgttctgaa aatgaccgac gaagccggtg
     4681 aagatgcgaa actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta
     4741 aagacgtaaa cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact
     4801 acaaagacct cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg
     4861 ctaaagctga aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc
     4921 aataaccctg aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa
     4981 ctctctattc caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca
     5041 aacccgacca ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat
     5101 actcaccgtt caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt
     5161 tttttatgcg attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt
     5221 tacaaaaaaa aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt
     5281 ttattattac ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac
     5341 gggtcgttga taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc
     5401 gtattcaccg cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg
     5461 ataaacttca ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct
     5521 atacaactgg tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg
     5581 actgttgcct tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg
     5641 aactgataga gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta
     5701 ggcatcaggc gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat
     5761 ttcacttgca gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact
     5821 cccagggtgt cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc
     5881 tggccacttt gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc
     5941 tgatcgctct ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc
     6001 gccgggtcag agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg
     6061 gcgattacat cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt
     6121 tgatccggcg gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag
     6181 gcatggaagc gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc
     6241 tgtacgacgt taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca
     6301 ttgttgccaa cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct
     6361 tccggttgca ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc
     6421 ggcagatcgt gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac
     6481 aggtttggtg tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc
     6541 acggtgatcc gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg
     6601 ctccagtcgg catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca
     6661 agcgtcgagc gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc
     6721 ataaagttgc cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt
     6781 tgcacatcac cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg
     6841 gtaaagatgg ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga
     6901 ttacggcggc cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc
     6961 aggcggatgg tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt
     7021 gtctgaaacg ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc
     7081 cccgaaaggg tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc
     7141 gctttacccg tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg
     7201 gcgaggtcga agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt
     7261 tgcggcacgc acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt
     7321 tgctccttat tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc
     7381 gtctggaagc gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt
     7441 cctgccagat taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc
     7501 tgcttttcgc tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa
     7561 atttgctcaa cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct
     7621 tccacgcgaa cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct
     7681 gccagtagtt gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg
     7741 ccaagccccg gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga
     7801 tcttttaccc ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc
     7861 ccagggatca tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg
     7921 ttctgtttga cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg
     7981 tacaggctac cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct
     8041 ttggcggtgt aaatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag
     8101 tcgagcaacg ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga
     8161 ttgacctgct gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt
     8221 cccttcactg ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt
     8281 ttcagatcat ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg
     8341 ttcacttcca cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc
     8401 atatccaccg gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc
     8461 actttcagct tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg
     8521 atatccaccg gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg
     8581 ttggaatcga tatccaaggc gtccagttta gtattgccgt caatgctgct cactttcagc
     8641 agcatggtgc gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac
     8701 tcttcaatgt tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga
     8761 gaaaagagat ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt
     8821 ttcggttcga ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg
     8881 cctttcagcg acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg
     8941 aagtccatca ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc
     9001 cgtgtcaggg tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct
     9061 tcaacctgtt cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc
     9121 gccaggtcat taatacaaac gctactgttc cacaggcact caagaccgac cgccagatgc
     9181 agattacccg cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga
     9241 tcgcgccagc cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct
     9301 ttaaatacaa gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac
     9361 agtaagataa cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc
     9421 ccagaccgat gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat
     9481 cgagtttgat tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa
     9541 agtcgctgcg gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc
     9601 attttccggt cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt
     9661 tcaggtcacc gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc
     9721 gatcgccccc gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa
     9781 tccagcccag cgtaccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga
     9841 cgttctgcgc ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt
     9901 cgatagagta gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc
     9961 ggctaatcat caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa
    10021 agtggtcgag gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt
    10081 agcgagaagc caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa
    10141 aaccgccctg caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc
    10201 tgaagtcgag ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt
    10261 atgagttcat ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt
    10321 aaccgccccc ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta
    10381 atactttcgt ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc
    10441 cggtagcaga aagtcggcgg ttcagttccg ccaaatcttt cgattcatac tcatcgccct
    10501 ctttaaacgg caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca
    10561 catgcccaaa gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca
    10621 ggccgagcgc aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca
    10681 acgcaatact ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac
    10741 caatagccgg gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc
    10801 gcaataccac atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca
    10861 atacctgccg ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat
    10921 aacccagcgc tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt
    10981 ctggcgtcac ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct
    11041 gtcccgataa cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc
    11101 agagtaagct tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt
    11161 ttttcctgcc cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa
    11221 aagcagacta aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat
    11281 tgtgtgcaaa tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg
    11341 acaccatgag tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac
    11401 gtaacacccc gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg
    11461 tacctgacgg aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc
    11521 tgttctggca gttacccggc ctttacagca ccgccgcagg ctataccggc ggctatacgc
    11581 caaatccgac ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca
    11641 ttgtttacga tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc
    11701 acgatcccgc ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga
    11761 tttatccgct gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg
    11821 cggcgatgct tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac
    11881 cgttttatta tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttact
    11941 gtggaattgg cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat
    12001 gtagtttgtt gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg
    12061 cctacaaaat cgtccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc
    12121 gcatcaggca gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag
    12181 taccttacgc tatactagcg actgaaaatg ccggttcact ttcttcgaat cggctttcaa
    12241 tgtgtatttc acacaaatta atcaactttc ccttccgagg atctggcctg aaaggtcgga
    12301 taagatatgt taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc
    12361 tcgatgtccg agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat
    12421 gaaggcaata taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt
    12481 accgtggtcc aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg
    12541 gcattttctc cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag
    12601 caactgagct ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat
    12661 ttaaccccga aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac
    12721 ccgatgcgct tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg
    12781 aacatgatct tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat
    12841 gacatctacg cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac
    12901 gagctgattg aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg
    12961 cgtgaaaacg tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg
    13021 gcggaacatc cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt
    13081 tatgtcgatt ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac
    13141 agcggcgtac aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg
    13201 ttggaaagtt ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg
    13261 gtggtgggga tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt
    13321 caggggctgg aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc
    13381 accccaattg acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac
    13441 tacgaaacca tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat
    13501 tcggtgaaat tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac
    13561 cagctgctgg tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac
    13621 gctaaagata aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca
    13681 ggagccgtct tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa
    13741 cctgacggtt aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa
    13801 gaatcttacc cttatcaaat tcaaaagcgc caacgtcctt gatgtataac cgtccacgga
    13861 acaggatctt tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg
    13921 ttatctcctg ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc
    13981 gcaaatttta atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact
    14041 ttttttacct aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag
    14101 tataagcatt catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc
    14161 gggaaattcg ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg
    14221 catatcataa gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct
    14281 gttgccgatg atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg
    14341 cattaccgat cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa
    14401 cagcgcgcag ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc
    14461 aaaagagaaa aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg
    14521 ttaccagacc accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt
    14581 gcgcagcagt ctggagagta ataaaaagct ttatcccagg tcgcagttta ttgtcgatag
    14641 caatggcgtc gcgcgcggtg cctggcagct ggatgacgaa agttccgctg tagtggtgct
    14701 ggataaagac ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca
    14761 gcaagtgatg gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa
    14821 aaataatttt cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca
    14881 ccgaactaat actggtaacg ctagttgctc ttttttaaac gcctcaatga tcgatttata
    14941 tttctcgcaa atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat
    15001 cgcatccact tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata
    15061 aataagagag taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc
    15121 ccctccatag attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc
    15181 aattattttt tcgtaaatat accatccatc acattcttta attccaccgc ctggtatttg
    15241 gagttcaaat ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa
    15301 agatacgtca ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg
    15361 ttttttctgc aaaatatcac aggcat
//
LOCUS       DAJVQH010000096        15350 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJVQH010000096 DAJVQH010000000
VERSION     DAJVQH010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.096"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..1115)
                     /locus_tag="OQM87_002983"
     CDS             complement(<1..1115)
                     /locus_tag="OQM87_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1509048.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQER"
     gene            complement(1314..1398)
                     /locus_tag="OQM87_002984"
     tRNA            complement(1314..1398)
                     /locus_tag="OQM87_002984"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1362..1364),aa:Leu,seq:caa)
     gene            1594..2613
                     /gene="ahr"
                     /locus_tag="OQM87_002985"
     CDS             1594..2613
                     /gene="ahr"
                     /locus_tag="OQM87_002985"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCS1509049.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2742..4244
                     /locus_tag="OQM87_002986"
     CDS             2742..4244
                     /locus_tag="OQM87_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCS1509050.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4363..5445)
                     /gene="lptG"
                     /locus_tag="OQM87_002987"
     CDS             complement(4363..5445)
                     /gene="lptG"
                     /locus_tag="OQM87_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1509051.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5445..6545)
                     /gene="lptF"
                     /locus_tag="OQM87_002988"
     CDS             complement(5445..6545)
                     /gene="lptF"
                     /locus_tag="OQM87_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1509052.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPIRRLRASFSRKGAV"
     gene            6812..8323
                     /gene="pepA"
                     /locus_tag="OQM87_002989"
     CDS             6812..8323
                     /gene="pepA"
                     /locus_tag="OQM87_002989"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCS1509053.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8457..8900
                     /gene="holC"
                     /locus_tag="OQM87_002990"
     CDS             8457..8900
                     /gene="holC"
                     /locus_tag="OQM87_002990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCS1509054.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8900..11755
                     /gene="valS"
                     /locus_tag="OQM87_002991"
     CDS             8900..11755
                     /gene="valS"
                     /locus_tag="OQM87_002991"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCS1509055.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11809..13005)
                     /locus_tag="OQM87_002992"
     CDS             complement(11809..13005)
                     /locus_tag="OQM87_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCS1509056.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSAGMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNSVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILTFLDLLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13198..13701
                     /locus_tag="OQM87_002993"
     CDS             13198..13701
                     /locus_tag="OQM87_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1509057.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13747..14163)
                     /gene="rraB"
                     /locus_tag="OQM87_002994"
     CDS             complement(13747..14163)
                     /gene="rraB"
                     /locus_tag="OQM87_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCS1509058.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14325..15338
                     /gene="argF"
                     /locus_tag="OQM87_002995"
     CDS             14325..15338
                     /gene="argF"
                     /locus_tag="OQM87_002995"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCS1509059.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3956 a         3911 c         3957 g         3526 t
ORIGIN      
        1 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
       61 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      121 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      181 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      241 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      301 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      361 tctacttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      421 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      481 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      541 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      601 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      661 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      721 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      781 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      841 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      901 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
      961 gcaagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1021 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1081 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1141 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1201 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1261 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1321 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1381 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1441 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1501 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1561 acttcccaaa caacaccaga gaaggaccca aaaatgtcga tgataaaaag ctatgccgca
     1621 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1681 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1741 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgtg
     1801 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1861 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1921 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     1981 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2041 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2101 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcat
     2161 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2221 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2281 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2341 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2401 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2461 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2521 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2581 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2641 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2701 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2761 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2821 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2881 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     2941 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3001 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3061 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3121 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3181 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3241 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3301 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3361 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3421 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3481 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3541 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3601 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3661 ttccggataa tgtgctcggc cagctcggta atcgtgttca gcacgctttg cgagctttta
     3721 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3781 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3841 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3901 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     3961 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4021 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4081 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4141 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4201 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4261 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4321 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4381 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4441 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4501 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4561 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4621 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4681 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4741 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4801 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4861 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4921 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     4981 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5041 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatcg ccatcgtcag
     5101 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5161 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5221 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5281 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5341 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5401 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5461 cgcgaaaaac tggcgcgcag gcggcggatc ggcacggtgt cccaaaggtt gagaacaatc
     5521 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5581 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5641 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5701 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5761 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5821 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5881 aaatcacgta acagtgcagt gccttcgaag cgtgttccct ggttgagagt gacgacctgg
     5941 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6001 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6061 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6121 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6181 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6241 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6301 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6361 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6421 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6481 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6541 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6601 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6661 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6721 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6781 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6841 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6901 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     6961 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7021 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7081 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7141 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7201 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7261 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7321 tgagcgcgcg atccagcacg gtctggcgat tgccgccggg ataaaagcag cgaaagatct
     7381 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7441 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7501 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7561 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7621 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7681 gaagtacgat atgtgcggcg cggcggcggt ttacggcgtg atgcgtatgg tcgcggagct
     7741 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7801 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7861 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7921 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     7981 tcatattact ggtctgatgg cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8041 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8101 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     8161 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8221 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8281 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8341 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8401 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8461 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8521 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8581 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8641 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcggtgcg ccggtggaga
     8701 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8761 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8821 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8881 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     8941 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9001 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9061 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9121 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9181 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9241 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9301 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9361 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9421 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9481 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9541 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9601 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9661 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9721 ctgcgtgaag atcaccccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9781 cctgccgatg atcaacatcc tgacctttga cggtgatatc cgtgaaagcg cccaggtgtt
     9841 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9901 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
     9961 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10021 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10081 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10141 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10201 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10261 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10321 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10381 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10441 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10501 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10561 gatgtccaaa tccaagggta acgttatcga cccgctggat atggttgacg gtatttcgct
    10621 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10681 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10741 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10801 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10861 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10921 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    10981 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11041 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11101 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11161 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11221 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11281 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11341 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11401 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11461 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11521 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11581 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11641 ctttgttgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11701 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11761 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11821 taaagaaggc atgactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11881 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    11941 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    12001 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12061 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12121 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12181 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12241 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaag gtaataaatc
    12301 taaaaatgtg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12361 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12421 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12481 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12541 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12601 aaaactaaat cttacgctgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12661 tgccataaat ataagcaaaa ccaacagtaa taaagtcaaa acacagccaa caagcggcat
    12721 acctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
    12781 aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc
    12841 ataaagataa cgcttacatt tcattaatgc ccatggtaaa tagataccta gcgttataat
    12901 cgttaacaac acattcacca cacaaataag aaaatatctc tcacctgtac catgaaaaac
    12961 aaaagaatgg gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat
    13021 taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa
    13081 tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt
    13141 aagaagagag tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg
    13201 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct
    13261 atcgccagcg tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac
    13321 accgttgccg atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct
    13381 tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca
    13441 ggtagtgaag cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt
    13501 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggctcgaga gatgggcttc
    13561 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    13621 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg
    13681 cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc
    13741 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc
    13801 ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc
    13861 cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
    13921 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
    13981 ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc
    14041 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
    14101 taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc
    14161 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg
    14221 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat catacacata
    14281 aatttaattt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
    14341 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
    14401 tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta
    14461 aaaacatcgc gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg
    14521 ccgcatatga ccagggtgct cgcgttactt atcttggccc aagcggtagt cagattggtc
    14581 ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt
    14641 atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc gtgccggtat
    14701 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
    14761 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
    14821 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
    14881 tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg
    14941 cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg
    15001 ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg
    15061 aacgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
    15121 acccggaggt caaattcctc cactgcctgc ccgcgtttca tgacgaccaa acgacgcttg
    15181 gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct
    15241 tcgagtctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag
    15301 cggtgatggt cgcgacgctc agtaaattaa ataattaata tctctataaa
//
LOCUS       DAJVQH010000097        15185 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJVQH010000097 DAJVQH010000000
VERSION     DAJVQH010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.097"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(1..592)
                     /locus_tag="OQM87_002996"
                     /pseudo
     CDS             complement(1..592)
                     /locus_tag="OQM87_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
     gene            complement(995..1240)
                     /locus_tag="OQM87_002997"
     CDS             complement(995..1240)
                     /locus_tag="OQM87_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509060.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1885..1960)
                     /locus_tag="OQM87_002998"
     tRNA            complement(1885..1960)
                     /locus_tag="OQM87_002998"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1925..1927),aa:Phe,seq:gaa)
     gene            complement(2066..2773)
                     /locus_tag="OQM87_002999"
     CDS             complement(2066..2773)
                     /locus_tag="OQM87_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCS1509061.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3171..5306
                     /gene="speC"
                     /locus_tag="OQM87_003000"
     CDS             3171..5306
                     /gene="speC"
                     /locus_tag="OQM87_003000"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS1509062.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LVIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVVMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(5355..6611)
                     /gene="nupG"
                     /locus_tag="OQM87_003001"
     CDS             complement(5355..6611)
                     /gene="nupG"
                     /locus_tag="OQM87_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCS1509063.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6813..7892)
                     /gene="mltC"
                     /locus_tag="OQM87_003002"
     CDS             complement(6813..7892)
                     /gene="mltC"
                     /locus_tag="OQM87_003002"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCS1509064.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7957..8232)
                     /locus_tag="OQM87_003003"
     CDS             complement(7957..8232)
                     /locus_tag="OQM87_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCS1509065.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(8260..9312)
                     /gene="mutY"
                     /locus_tag="OQM87_003004"
     CDS             complement(8260..9312)
                     /gene="mutY"
                     /locus_tag="OQM87_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCS1509066.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQMLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPKFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            9473..10192
                     /gene="trmB"
                     /locus_tag="OQM87_003005"
     CDS             9473..10192
                     /gene="trmB"
                     /locus_tag="OQM87_003005"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCS1509067.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            10192..10518
                     /locus_tag="OQM87_003006"
     CDS             10192..10518
                     /locus_tag="OQM87_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCS1509068.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10702..11421
                     /locus_tag="OQM87_003007"
     CDS             10702..11421
                     /locus_tag="OQM87_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCS1509069.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11597..12643
                     /gene="ansB"
                     /locus_tag="OQM87_003008"
     CDS             11597..12643
                     /gene="ansB"
                     /locus_tag="OQM87_003008"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCS1509070.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12760..13767
                     /locus_tag="OQM87_003009"
     CDS             12760..13767
                     /locus_tag="OQM87_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCS1509071.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13823..15124)
                     /locus_tag="OQM87_003010"
                     /pseudo
     CDS             complement(13823..15124)
                     /locus_tag="OQM87_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         3934 a         3649 c         3915 g         3687 t
ORIGIN      
        1 gaggcctttg ccctcagtcc ctgacggcgc aggcttgccg ccacggtttt tacgttaaag
       61 gggtaaccct gagcacgcag ttcatccgtc aggcgtgggg caccgtaacg ctgttttgac
      121 cgggtaaaag ccgcgaggac aacgctgtcg cagtgttggc ggaactgctg acgcgtgctt
      181 atccttgtcc gccgctgaca ccacgtatac cagccgctgc gggccacccg gagcacgcgg
      241 cacattgctt tgatgctgaa ctcagcctga tgtttttcaa taaagacata cttcatttca
      301 ggcgcttcgc gaagtatgtc gcggcctttt ggaggatagc cagctcttca tcccgttctg
      361 ccagctggcg tttgagacgt gcaatctcgg tagacatctc cagttcacgt tcagaagacg
      421 tctgctgatt ttgctgttta ctgcgccagt tgtagagctg tgattcatac aggctgagtt
      481 cacgggctgc ggcagtaaca ccgatgcgtt cagcaagctt cagggcttca ctgcgaaatt
      541 caggcgaatg ctgtttacgg ggttttttac tggttgatac tgtttttgtc atgtgagtca
      601 cctctgactg agagtttact cacttagccg cgtgtccact attgctgggt aagatcaggc
      661 tgaggagtcc ccagaaaaga agacaggcat agagttttgt gatctactga ttgcgctaac
      721 aaattcaatg agatcacctc tatgcctgcc cgttaagtat gtcaggcttt tttccgtaat
      781 gctctaactt ccacgcattg gtttctgaca tcaaataagg ctatgacttt ctctcccttt
      841 taatctcatt ctgatctaaa accatatctt aaaaaaatgc atgggtattt taattatttt
      901 gatgttgatc taacgcactc aaggaccatt ttattaccga ttatccattc tatttaactg
      961 ttgcgatata aatgtttgtt ttaggtgctt taattcagta caagtcatca aaccgtacca
     1021 gaccctgact gtcagtgaca ccgacaggtg ggggcggcgg tgtggggtca aactgagtgt
     1081 gtctgcctct ggcccacaga gccagctctg ccgccattgc ccgtggatga tactccagct
     1141 gcacgttgag accggaaact gggttgttgt tctcatccac gaactgcagc gccagccagt
     1201 gcatccgctg cttaccgcag gcgacttctt tgtacttcat tttgtgactc aggcaggtga
     1261 ctgtcgtgtt gccggttgcg tccattttgc ggttaaaccc ttttaattca atttgttaaa
     1321 ttgctttcca ccttttgcgg ggcgcgactt aaccaaaata ttcactgtta tcataatatg
     1381 caatgggctg gaggttgcag gtcatttagg gattttctgt aagccccgtt ttgcagaacg
     1441 ttctatctcc gattgagatc ctgtctctcg gtattaccgg tagtctggcc ataatcccct
     1501 atggatcata ttgatatagt aggcataaca cccaaaagtt gactaaagct aaatcaatca
     1561 gagtgaaaaa tgttcggttt gatgtagggg gggactgagg aggggccaac gcaggtggta
     1621 tgcgttttac ttcagaccgg acaaagaccg gatgtatccg tgcagtaatg aatccgttag
     1681 ccaggtgctc atgtggaaat agtgtctatt ggggatttga agtttgacag aaaaggccac
     1741 agtgaccaga agggggctac ttagaagaaa tgttcaggct ggatggaaga ggtaaagcag
     1801 tgacatcagc attcaaagtg accggatatc taccagaacc ccagaaagca aaaaaccagc
     1861 ccgtaggctg gttcttctaa gtagtggtgc ccggactcgg aatcgaacca aggacacggg
     1921 gattttcaat cccctgctct accgactgag ctatccgggc aacggggcgc attaaacctg
     1981 attcgcctcg tctcgtcaat caaatttctt caatttactg cagactgcac aatctatcat
     2041 cactttgccg ctattgcacg cattgtcagg caaaccaggc ggtccaggcg gcggaaagcg
     2101 gcattgccag aaggagtgct ggaagcatgt tgaccaccgg gaacatttta atgccacaga
     2161 tgcgtaatcc ggttgccagc agcaataaac cgcctacggc gctgaagtct gccatcatcg
     2221 acggtgtggt cagcggtaat atcagcgcgg cagcccacgc cagcgttaac tggatgatca
     2281 gtaatgggat actaattacc gacaccgcaa tacccagcga gcaggcgaag atcatcgccg
     2341 taaagaaatc aagaaatgac ttggcgatta aaatactcgg atcgccggtc atcccttcgt
     2401 tcatcgcccc gaagatcccg gtgccgctgg cgcaaaacag gacaataatc gcgacataat
     2461 tctgaataaa agattcatgc gctggcttct tacgtgagtg acgaaacaga ttttgtgctt
     2521 tggcgactgc tgtattgaca cctttctcca gcaaacaaat ttcgccaatt agagcgccaa
     2581 gtagcgtagc taaaaccatc gctggaaggt tggcgcattt cacaaccagt aaaataccaa
     2641 tccccagcga tgccagacca aaaatagatg tcatggagac gcggatacgt tccggtaagc
     2701 gttggctgag cagtgcgccg agaacgccac ccagtaagac tgcactagcg ttgataaaag
     2761 ggccgatgac cacaagagtt cctgttagct gtattgtttg attttcttat tatgaacttt
     2821 ttgccttgtt gatggcttgt ctggtcgcaa agtcctggct tgcacgcctt agcgaaaggt
     2881 gccatgattg cgcgaatttt ctcctctctg tacggagttt gcccgatgca cgccatctcc
     2941 ttacattctc tcgcttatcg ccgtttcgcg cgaaacgttt ccctttctat gttactgctc
     3001 atgcggtgaa gttaacgcac gctcactgca ggacaacagt aaaatcagag cgtttctgct
     3061 tttactgatg tctggcggtc ggagctggtg accagtttga cccatatctc atggggcagg
     3121 gttttccacc ttgtccggta ttcttacttc cccgaaacgg gtttgcgctt atgaaatcaa
     3181 tgaatattgc cgccagtagt gaactggtat cccgactttc ttctcatcgc cgcgtggtgg
     3241 cgttgggaga tactgatttt acggacgtcg cggcagtcgt cattaccgct gcggatagtc
     3301 gcagtggcat tcttgcgttg cttaagcgca ccggttttca tctaccggtg tttttgtatt
     3361 ccgaacatgc tgttgaatta cctgcgggcg ttacggcggt aatcaacggc aacgagcagc
     3421 agtggctgga gctggaatcc gcagcctgtc agtatgaaga gaatttgctg ccaccgtttt
     3481 atgacacgct gacgcagtac gttgagatgg gcaacagcac ctttgcttgc cctggacatc
     3541 aacatggtgc gttctttaaa aagcatcctg ccggacgcca tttttacgat ttctttggtg
     3601 agaacgtctt tcgcgccgat atgtgtaacg ctgacgtaaa attgggcgat ctgcttattc
     3661 atgaaggatc ggcgaaagat gcgcagaaat tcgcagccaa agtctttcat gccgataaaa
     3721 cctattttgt gctgaacggc acatcggcag cgaataaagt ggtgacgaat gcgctgttaa
     3781 cgcgtggcga tctggtgctc ttcgaccgta acaaccataa gtcgaatcat cacggcgcgc
     3841 tgattcaggc ggggacgacg ccggtctatc tggaagcttc acgtaacccg tttggtttca
     3901 ttggcggtat tgatgcgcac tgttttaatg aagagtatct gcgccagcaa attcgcgacg
     3961 ttgcgccaga aaaagccgac ctgccgcgcc cgtttcgcct ggtgattatt cagctgggaa
     4021 cctatgacgg cactgtctat aacgcccgtc aggtgatcga taccattggg catctctgtg
     4081 attacattct gtttgattcc gcgtgggtcg gttacgaaca gtttatcccg atgatggcgg
     4141 atagctcgcc gctgctgtta gaacttaacg aaaacgatcc ggggattttt gtgacccagt
     4201 tggtgcacaa acagcaggct ggattctcac agacgtcgca gatccataaa aaagataacc
     4261 atatccgcgg acaggcgcgt ttttgcccgc ataagcggtt gaataacgcc tttatgctcc
     4321 atgcttctac cagccctttc tatccgctgt ttgctgcact ggatgttaac gccaaaattc
     4381 atgaagggga gagtgggcgt cggctgtggg ctgagtgcgt tgagttgggt attgagtcgc
     4441 gcaaggctat tcttgcgcgc tgtaagctgt tccgcccgtt tatcccgccc gttgttgatg
     4501 gcaaattgtg gcaggattat ccgacatcag tgttagccag cgaccgccgt tttttcagtt
     4561 ttgagccggg ggcgaagtgg cacggctttg aaggatatgc cgcggatcag tattttgttg
     4621 atccgtgcaa gctgttactc actacaccag gtatcaatgc cgaaaccggc gaatatagcg
     4681 actttggcgt tccggcgacg attctggcgc actatctgcg tgagaacggc attgtgccgg
     4741 agaagtgcga tctcaactcc attctgttct tattaactcc ggcggaaagc cacgagaagc
     4801 tggcacaact ggtggtgatg ctggcgcaat ttgaacagca tattgaggat gactcgtcgc
     4861 tggctgaggt gttgccgagc gtttataaca aatatccggt gcgctatcgc gactacaccc
     4921 tgcgccagtt gtgtcaggag atgcacgatc tgtatgtcag tttcgacgtc aaagacctac
     4981 aaaaagcgat gttccgccag cagagtttcc cgtcagtggt gatgaacccc caggatgcgc
     5041 atagcgctta tattcgcggt gaagtggagt tggtgcggat tcgtgatgcc gaagggcgaa
     5101 ttgcggcaga aggggcgttg ccttatccac ctggcgtgct ttgcgtggta cccggggaag
     5161 tctggggtgg ggcggttcaa cgttatttcc ttgcgctgga agaaggcgtg aatttgttgc
     5221 cgggattttc gccggagttg caaggtgtct atagcgaaac cgatgcggat ggcatgaaac
     5281 gattgtacgg ttatgtgttg aagtaagaat aaaaaaacgg gtcaccttct ggcgacccgt
     5341 ttttctttgc gtaattagtg gctaaccgtc tgtgtgcctg tcgggacacg aacgtgttta
     5401 tatttgaaca tcgccatgaa cgcgaaggcc agaaccacgg aataaccagc gaaaatcaac
     5461 catacggtct gccagtcggt aatgccgttt tgggtgtaca tctcaacaac tttaccgctc
     5521 acgatgccgc cgaggataca gccgaagccg ttagtcatca tcaggaacgt cccttgtgca
     5581 ctggcgcgaa ttgccgggct aacttctttt tcgacaaaca ccgaaccaga gatgttgaag
     5641 aagtcgaatg cgcaaccgta aacgatcatc gacagtacca gcagtacagt accgaacgga
     5701 gtcgggtcgc cgtaagcaaa cagcgcaaaa cgcaggatcc acgccacaat actgatcatc
     5761 attacgttct taataccgta gcggcttaag aagaacggga tggtcagaat gaacagggtt
     5821 tcagagatct gcgaaatcga catgatgatt gacgcatgct gcacgatgaa gctgctggca
     5881 aacatcggat ctttgtcgaa gctgtgcagg aaggtattac cgaacatgtt ggtaatctgc
     5941 agttccgcgc ccagcagcat tgagaagatg aagaagattg ccatacgctt gtttttaaac
     6001 agcgcgaatg catcgaggcc cagcagggtt gtccagctct gattcgcttg ctgtttagca
     6061 accggaatat gcggcagagt cagggtaaac agaaccagaa tggcggaaag tgctgcgcca
     6121 atatacagct gcatgtggct taattcgaag ccagacaggc tcaccaccca cattgccatg
     6181 ataaagccga tggtgcccca gatacggatt ggcgggaagt cagtaacgat atccatcccg
     6241 gcattttgca ggcgatagta agagatggtg ttgattaacc caagcgttgg catataagca
     6301 aacgagttaa tcaatatcac aaggaacatc gcttccggcg tcgtgacctg tgccgccatg
     6361 aacagcgtga tagcgccaat ggtgtggcaa atggcatata cccatttcgc acttaaccat
     6421 ttgtcggcca caatccccag tagcgcaggc ataaataccg cggcgatacc cagtgagcta
     6481 taaactgcgc caatagaagc accgtcaaac ttcagggtaa caaacatata ggagccgagg
     6541 gtcgtcagcc aacttcccca cagacagaac tgcagaaaag agaggatttt cagctgcagc
     6601 ttaagattca tgttaatttc ctcacatcgt gattcggatg agtgtttgaa aaagcacatt
     6661 tgaccgtgcg agatgatggg attctatcaa cttcgcggac tggttttttg ttacctgtgg
     6721 caaataattg caaacattcc caatttgcaa aacgaaattg tgatgtggat aacatttttg
     6781 cccctgagca tcgtcagggg cggttaatgg aattatcggc ggcggtagga tttttgcgcg
     6841 gtattcactt tataaagata acggcgagat tccgcagagg gatggcgggt cgtcagcgtc
     6901 tgataaacat cgcccggcgt catggtgtta ataatattgg cagcctgaat cttatcattc
     6961 gaaaagactc gcagcacgct gcctgcgccg ccgttatagg cggtgatgac ggcataacgc
     7021 cgcgatgttg ggttatcaat tccgccgaga taaacattgt tcagcatcgc cagatacgcg
     7081 gtgccggtat caatattgct ggcaggatca aacaagaaac tgcggctcgg cgtgccggat
     7141 ttcccctgcg agcggaacac atctttcccg gcagtatttt gtaccacctg cattaatccc
     7201 agcgcatcgg aacggctgac cgcatacggg ttaaaggaag attcagtctg cataattgcc
     7261 agaatcagcg actcatcaac gccatatttc cgtgacgcct ggcggaccat gccgagatat
     7321 ttgtgcgcac gtttatcaag gtggttcggc accatgttaa tggtgacgct gtagatgata
     7381 cgtagcccgt tgctgcggct tttcagacgg tttttcagca gataatccgc gaagttgctt
     7441 gcgcgacctt cccagcgaat cggctgcccg gtgttgtcca ccacctgacc gtaaaggaaa
     7501 ggttctttcg aaattgtaat atcatcaacg tcggaataga gatcgaccga actcggatcg
     7561 tcacccatca gcaacgtttt gataattgcc cggcgcaaat gcgcggcagg ttctgtcccg
     7621 gcgatggttt cgatagtaat cgtaccgtca tcgaagttga tgtggctgcg ggtctgatat
     7681 tgatcggtgt atttcacgta gtccttagga ccggcgatca ccacctcttt gaagccccag
     7741 atgttctcaa tattgtgggc aaattgcccc atcagaatat caaaaccgtt ggtatctttg
     7801 acccaggctt cgttataggt atcgcctttt ttggtcgtcg aacaggagat gagcaacggc
     7861 gcaatcaaag ccagcgcgag atattttttc atcattccgg gtgcgtgttg tgtttgtttt
     7921 tatgcaagct gccggaggcg tgctccggca ctgtttttat tttttatctt ccggcgtata
     7981 gccctcgata tgcacctctt taccctcgaa caggaagttg accatctcct gctccagcag
     8041 cttgcggtgc tcggcattca tcatgttgag tttcttttca ttaatcagca tggtttgttt
     8101 gtgctgccac tgcgcccagg cttctttgga gatctcgtta tagatgcgtt ttcccagctc
     8161 gccggggtac agctgaaaat cctgaccttc tgcttcacgt tgcaggaaag tacaaaaaat
     8221 cgttctgctc ataaatcatc ctctttatcg actcacgcgc taaaccggcg cgccagtgcg
     8281 taactgctgt aacaaacgct ccacgggagc cgccaggcca actgacggcg gttgcgctaa
     8341 gttataccag agcgcattgc cttcatccat gcagccggtg aatgacgaca cgggaagcca
     8401 cataggcaca atatctaagt ggaaatggct gaaggtatgg cgaaacgcgg tcagttgcgt
     8461 caggttatcg gcagcaatct gccgttgtgc cagccaatgc cgcaaacttt cttcgtcggc
     8521 aaacttcggg aaacagtata aaccgcccca caatccgctc ggcggacgct gcgccagcaa
     8581 tacttcatct tcgtgctgta acaggagaaa gtagccggtg cgctccggca gcatctgttt
     8641 cggttttttg cccggataaa gcgaccagct attgttggtg gcggcaatac atccgttttg
     8701 tagcggacag agcgaacatt tcggcttcga acgcgtgcaa atcatcgcgc ccaaatccat
     8761 catcgcctga ttaaaccgtt ccacgccgac cgcgggcgtc acctgctcgc tcaaactcca
     8821 tagtttattc tcgacctctt ttttcccagg ccagccgctt acagcatagc agcgcgccag
     8881 tacgcgtttg acgttaccgt cgagaatcgg aaagtgcttg cccagagaaa gcgagagaat
     8941 cgcgcctgcg gtggaacgcc cgacgcccgg taacggcgcg acttcttcaa aggtttccgg
     9001 gaatttacca ccgtgtaagg tcgccacttg ttgtgccgct ttatgcaggt tgcgcgcgcg
     9061 ggcgtaatag ccaagcccgg tccacaagtg gagaacttcg tcgagcggcg cattggcgag
     9121 atcggtcacc gtcgggaagt gcgccataaa gcgttcaaaa taggggataa cggtcgcaac
     9181 ctgagtttgt tgcaacatca cttctgagag ccatactttg tagggcgtct tgtcaatttg
     9241 ccagggcagc gttttccgcc cgtatttatc gtaccagtcc agaacctggg ctgaaaattg
     9301 cgacgcttgc atggtcaccg acttcactgt tgttgggggc aagattgcag cacagcgacg
     9361 gcagggtgta aaccggaact ttccgcagcc acggccttat catgcttgca tccggcaact
     9421 aactttggat aatgcccgtt ttcagaacac tttcacaagc gactaaatct ttatgaaaaa
     9481 cgacgtcatt tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta tccgtagttt
     9541 tgtgcgccgc caggggcgac tgaccaaagg ccaggaacat gcgctggaaa attactggcc
     9601 ggtgatgggc gttgagttca gtgaagatat gctggatttc cccgcgcttt ttggccgtga
     9661 agcgccggtg acgcttgaga ttggttttgg catgggggcg tcgctggtgg caatggctaa
     9721 agatcgcccc gagcaggact tcctcggcat tgaagtgcat tcaccgggcg ttggtgcgtg
     9781 cctggcttct gcgcatgaag aaggtttaag caacctgcgc gtgatgtgtc acgatgcggt
     9841 tgaagtgctg cataaaatga ttcctgacaa ttcattgcgc atggtgcagc tctttttccc
     9901 tgacccgtgg cacaaagcgc gccataataa acgccgtatc gttcaggtgc cgtttgccga
     9961 actggtaaaa agcaaactgc agctgggggg cgtattccat atggcgaccg actgggaacc
    10021 ttatgcggaa catatgcttg aagtgatgtc ttctattgac ggttataaaa acctgtcaga
    10081 gagcaatgat tacgtaccgc gtccggcatc acgtccggtg acgaaatttg aacaacgtgg
    10141 tcatcgtctt ggtcacggag tatgggactt aatgttcgag agggtgaaat aatggcaaag
    10201 aaccgtagcc gtcgtctgcg taaaaaaatg cacatcgacg aattccagga attaggattt
    10261 tcggtggcat ggcgattccc ggaaggtaca tcggaagaac agattgataa aatcgttgat
    10321 gactttatta acgaggttat cgaaccgaac aaactggcct ttgacggcag cggttatctg
    10381 gcctgggaag gtctgatctg catgcaggaa atcggcaaat gcaccgaaga acatcaggcg
    10441 attgtgcgta agtggctgga agagtgcaaa ctggatgagg tacgcaccag cgaacttttc
    10501 gacgtttggt gggactaaga aagcatacgg gcgatgacaa atgcaaaact gcctgatgcg
    10561 ctacgcttat caggcctaca gagatgcacg atcgagtagg cgggataagg cgtttacgcc
    10621 gcatccggca tggaaaacgc gtactttgtt atcaatctgg ggccagcata tgctggcctg
    10681 atttgttctt gagggaagac tatgatgcgc aaaatgctgc tggcggcagc actttcagtg
    10741 acggcaatga ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga cgatgtgatt
    10801 gtcagcccgc aaaccgtgca ggtgaagggc gaaaacggca atctggtgat cacgccagac
    10861 ggcaacgtga tgtataacgg taagcaatat tccctgaatg ccgcccagcg cgagcaggcg
    10921 aaggattatc aggctgaact acgtagcacg ctgccgtgga ttgatgaagg cgcgaaaagc
    10981 cgcgtcgaga aagcccgtat tgcgctggat aaaattatcg ttcaggagat gggcgacagc
    11041 agcaaaatgc gcagccgtct gaccaaactt gatgcgcagc ttaaagagca gatgaatcgc
    11101 attatcgaaa cgcgcagcga tggcctgacg ttccactata aagccattga tcaggttcgc
    11161 gccgaaggcc agcaactagt gaatcaggca atgggcggaa ttttacagga cagcattaat
    11221 gaaatgggcg cgaaagcggt gctgaaaagc ggcggtaacc cattacagaa tgtgctggga
    11281 agcttgggcg gtctgcaatc ctcaatccaa accgagtgga aaaagcagga aaaagatttc
    11341 cagcagtttg gcaaagatgt ttgtagccgc gttgtgactc tggaagatag ccgcaaagcc
    11401 ctggtcggga atttaaaata atcctctatt ttaagacggc ataatacttt tttatgccgt
    11461 ttaattcttc gttttgttac ctgcctctaa ctttgtagat ctccaaaata tattcacgtt
    11521 gtaaattgtt taacgtcaaa tttcccatac aaagctaagg gataatgcgt agcgttcacg
    11581 taactggagg aatgaaatgg agtttttcaa aaagacggca cttgccgcac tggttatggg
    11641 ttttagtggt gcagcattgg cattacccaa tatcaccatt ttagcaaccg gcgggaccat
    11701 tgccggtggt ggtgactccg caaccaaatc taactacaca gcgggtaaag ttggcgtaga
    11761 aaatctggtt aatgcggtgc cgcaactaaa agacattgcg aacgttaaag gcgagcaggt
    11821 agtgaatatc ggctcccagg acatgaacga taatgtctgg ctgacactgg cgaaaaaaat
    11881 taacgccgac tgcgataaaa ccgacggctt cgtcattacc cacggtaccg acacgatgga
    11941 agaaactgct tacttcctcg acctgacggt gaaatgcgac aaaccggtgg tgatggtcgg
    12001 cgcaatgcgc ccgtccacgt ccatgagcgc agacggtcca ttcaacctgt ataacgcggt
    12061 agtgaccgca gctgataaag cctccgctaa tcgtggcgtg ctggtggtga tgaacgacac
    12121 cgtactggac ggtcgcgatg tcaccaaaac caacaccacc gacgtagcga ccttcaagtc
    12181 tgttaactac ggtcctctgg gatacattca caacggtaag attgactacc aacgtacccc
    12241 ggcacgtaag cacaccagcg atacgccatt cgatgtctct aagctgaatg agctgccgaa
    12301 agtcggcatc gtttataact acgctaacgc atccgatctt ccggctaaag cactggtaga
    12361 tgcgggctat gatggcatcg ttagcgctgg tgtgggtaat ggtaacctgt ataaatccgt
    12421 gttcgacacc ctggcaaccg ccgcgaaaaa cggcactgca gtagtgcgtt cttcccgcgt
    12481 accgacgggt gctaccactc aggatgctga agtggatgat gcgaaatacg gcttcgtcgc
    12541 ctctggcacg ctgaacccgc aaaaagcgcg cgtcctgctg cagctggctc tgacgcaaac
    12601 caaagatccg cagcagatcc agcagatttt caatcagtac taatcacctc gtcccggcat
    12661 cgtgccgggg ctttttcact tcagacttac gtccattgcc aattttcatt accctaatga
    12721 taatcaccgg aataaattat tccgcgcgag ggttttcggg tgaaaaagca atggattgtt
    12781 ggcacggcgc tgcttatgtt gatgactggt aatgcctggg cagatggcga accgccaact
    12841 gaaaatatct taaaagatca attcaaaaag cagtatcacg gcattctcaa gcttgatgcc
    12901 atcagcttaa aaaatcttga tgctaagggt aatcaggcca cctggtcagc ggaaggcgat
    12961 gtctcttcca gtgacgatct ctatacctgg gtcggtcagt tggcagatta cgaactgctc
    13021 gaacagacct ggacgaaaga taaaccggta aaattctcgg cgatgttaac cagtaaagga
    13081 acgcccgcgt ctggctggtc ggtgaacttt tactcttttc aggcggcagc cagcgatcgt
    13141 gggcgggtgg tcgacgatat caaaacgaat aataaatatc tgatcgtgaa tagcgaagat
    13201 ttcaattatc gctttagtca gcttgaatct gcgttgaata cccagaagaa ctcgattcct
    13261 gcgctggaaa aggaagtgaa agcgcttgat aagcaaatgg tggcggcgca gaaagcagcg
    13321 gatgcatatt ggggaaaaga tgcaaacggt aagcaaatga cccgcgaaga agcctttaaa
    13381 aaaattcacc aacagcgtga tgagtttaat aaacagaacg atagcgaggc gttcgcggtt
    13441 aaatatgaca aagaggttta tcaaccggcg attgcggcat gtcataaaca gagtgaagag
    13501 tgttatgaag tgccgattca gcagaaacga gatttcgata tcaacgaaca acggcgacag
    13561 acttttctgc aatcacaaaa actcagccgt aaattgcagg atgactgggt aacgctcgaa
    13621 aaagggcaat atccgttaac gatgaaagtt tcagaaataa acagtaaaaa agtcgcgatt
    13681 ttgatgaaaa ttgacgatat caaccaagct aatgaacgct ggaaaaaaga taccgaacaa
    13741 ctgcggcgca atggggtgat taagtaactc atgttaagtg cctttgccgg ataatataaa
    13801 aagttatccg gcaaattatt ttttacatta gccctagtag ctgtggaatg aacaaggata
    13861 tttgcggaat gtaagtgatc atcatcaacg ctactagcaa tgcagcataa aatggcagca
    13921 atggtttgat taaatgttga atcttcacac ccgagataga acaaccaaca aatagcgcac
    13981 tacccaccgg cagtgttaac agcccaatac aaaggttggc caccatcata ataccgaaat
    14041 gtactgggtc cattcccagc tcttgtgcaa tcggcaagaa gatcggtgta aagattagta
    14101 cagccggtgt catgtccata aagatgccga caattaataa gacgatatta atcagcagca
    14161 atatgatcag tgggttatca gaaatcccca tcaatgcgtc gctgatcata taaggaatat
    14221 cagcattagt cattgcccag gacatgccca cggaaaaacc gatcaacagt aatacaatcg
    14281 aagtggtaac aaccgactcc agaatcagtt tgggtaaatg gcgccatttt acttcccggt
    14341 aaatcagcac cgagagaata aatgtgtaga caacggcaat tgctgacgct tcagttgcgg
    14401 taaaaatgcc gcccagaatg ccgcccatca caatgaacac cagaagcaaa cttggtaagg
    14461 catccagaaa cgctttacag gcctgagcaa aggttgctct ttctgaaagg gggtagccac
    14521 ggcgtttagc aataatgccg cagactatca ttaccgcaag acccattaag atccctggca
    14581 aatatcccgc cataaacagc gatgctacag aaacgccgcc agctgtcagg gaaaacacaa
    14641 ttaacacgtt tgagggcggt atcagtaatc cagtaataca agatgagacg tttacagcgg
    14701 tagaaaatgc cggatcataa ccttctttgg tctgaatggg attcagtgtg ccaccaacgg
    14761 cagcagctgc cgcaacggct gaaccagaaa tggaaccaaa catcatattc gccaggacgt
    14821 tcacatgacc aagcgaacca ggaacgcggc caaccattac ctaggcgagg ttaatcaaac
    14881 gtatcgcaat accgccgctg ttcatcagtg ttccggcaaa gatgaaaaag gggattgcca
    14941 acagggcaaa gttatccagt ccattagcca gtcgttgcga aacagtgatt gccgcgatat
    15001 cccagggaaa catcaaaagc attgaagcta cagtggcaat accgattgag aacgaaatgg
    15061 gcacaccaat aaagaccagc acaaaaaaac agccaaacag tgtaatcgcg atataactat
    15121 ccattaactg gcctcctgcg caggttgttt cagttgttga atgctttgat taataaaaca
    15181 caagg
//
LOCUS       DAJVQH010000098        15028 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJVQH010000098 DAJVQH010000000
VERSION     DAJVQH010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.098"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..237
                     /locus_tag="OQM87_003011"
     CDS             <1..237
                     /locus_tag="OQM87_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCS1509072.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            247..885
                     /locus_tag="OQM87_003012"
     CDS             247..885
                     /locus_tag="OQM87_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCS1509073.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLGRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            888..2051
                     /locus_tag="OQM87_003013"
     CDS             888..2051
                     /locus_tag="OQM87_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1509074.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2135..3759
                     /locus_tag="OQM87_003014"
                     /pseudo
     CDS             2135..3759
                     /locus_tag="OQM87_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3876..4151)
                     /gene="yjfN"
                     /locus_tag="OQM87_003015"
     CDS             complement(3876..4151)
                     /gene="yjfN"
                     /locus_tag="OQM87_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCS1509075.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4300..4629)
                     /gene="bsmA"
                     /locus_tag="OQM87_003016"
     CDS             complement(4300..4629)
                     /gene="bsmA"
                     /locus_tag="OQM87_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCS1509076.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4811..5560
                     /gene="yjfP"
                     /locus_tag="OQM87_003017"
     CDS             4811..5560
                     /gene="yjfP"
                     /locus_tag="OQM87_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1509077.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5557..6312)
                     /gene="ulaR"
                     /locus_tag="OQM87_003018"
     CDS             complement(5557..6312)
                     /gene="ulaR"
                     /locus_tag="OQM87_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCS1509078.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6420..7484)
                     /gene="ulaG"
                     /locus_tag="OQM87_003019"
     CDS             complement(6420..7484)
                     /gene="ulaG"
                     /locus_tag="OQM87_003019"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCS1509079.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7839..9236
                     /gene="ulaA"
                     /locus_tag="OQM87_003020"
     CDS             7839..9236
                     /gene="ulaA"
                     /locus_tag="OQM87_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCS1509080.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9252..9557
                     /gene="ulaB"
                     /locus_tag="OQM87_003021"
     CDS             9252..9557
                     /gene="ulaB"
                     /locus_tag="OQM87_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCS1509081.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9567..10031
                     /gene="ulaC"
                     /locus_tag="OQM87_003022"
                     /pseudo
     CDS             9567..10031
                     /gene="ulaC"
                     /locus_tag="OQM87_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
     gene            10045..10695
                     /gene="ulaD"
                     /locus_tag="OQM87_003023"
     CDS             10045..10695
                     /gene="ulaD"
                     /locus_tag="OQM87_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCS1509082.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10705..11541
                     /gene="ulaE"
                     /locus_tag="OQM87_003024"
     CDS             10705..11541
                     /gene="ulaE"
                     /locus_tag="OQM87_003024"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCS1509083.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11541..12227
                     /gene="ulaF"
                     /locus_tag="OQM87_003025"
     CDS             11541..12227
                     /gene="ulaF"
                     /locus_tag="OQM87_003025"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCS1509084.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12356..12631)
                     /locus_tag="OQM87_003026"
     CDS             complement(12356..12631)
                     /locus_tag="OQM87_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1509085.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12958..13353
                     /gene="rpsF"
                     /locus_tag="OQM87_003027"
     CDS             12958..13353
                     /gene="rpsF"
                     /locus_tag="OQM87_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCS1509086.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13360..13674
                     /gene="priB"
                     /locus_tag="OQM87_003028"
     CDS             13360..13674
                     /gene="priB"
                     /locus_tag="OQM87_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCS1509087.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13679..13906
                     /gene="rpsR"
                     /locus_tag="OQM87_003029"
     CDS             13679..13906
                     /gene="rpsR"
                     /locus_tag="OQM87_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCS1509088.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13948..14397
                     /gene="rplI"
                     /locus_tag="OQM87_003030"
     CDS             13948..14397
                     /gene="rplI"
                     /locus_tag="OQM87_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCS1509089.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14468..>15028)
                     /locus_tag="OQM87_003031"
     CDS             complement(14468..>15028)
                     /locus_tag="OQM87_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1509090.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     RTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3509 a         3663 c         4050 g         3806 t
ORIGIN      
        1 ttgttaggtt acgttatccc attatccagt gcggcaatca atgcggtgag tattccagac
       61 tatttcgcct ggggcgggat tgcactggtg attcagttac tcgtttttgc tggtgtcaga
      121 ctttatatgc ccgcattaag cgaaaaaatt attaatcaca ataccgcagc aggaatgttt
      181 atgggaaccg ccgcgctggc tggcggtatt tttaacgcag cttgtatgac atggtaatgg
      241 acgatcatgg ccagaaaacg caaatcaaga aataacagta aaattggtca cggagcgatc
      301 agtcgcattg gtagaccgaa taatcctttt gaaccgcgtc gcaatcgcta cgcacaaaaa
      361 tacttaacgt tggcgctaat gggcggtgcc gcttttttcg tattgaaagg ttgtagcgat
      421 agtagcgatg tcgataacga cggcgacgga acgttttacg cgacagtgca ggattgtatt
      481 gatgacggta ataatgccga catttgcgcg cgtggctgga acaacgccaa agcggcattt
      541 tatgccgatg ttccaaagaa tatgactcag cagaactgtc agtctaagta cgaaaattgc
      601 tactacgaca atgttgaaca gagttggatc ccggtcgtct ctggattttt gttaggccgg
      661 gttattcgta aagatcgcga tgagccgttc gtctataaca gcggcggatc ctcatttgct
      721 tcccgccccg tctggcgcaa cacttccggt gattactcct ggcgcttcgg ttctggcaaa
      781 aaagagtctt actcttcagg cggctttacc accagaaaag cgtcgaccgt ttcgcgcggc
      841 ggctatggtc gttcttccag cgcccgtggg cattggggag gctaatcatg ctgagacaca
      901 acgttcctgt gcggcgggat ctggaccaga tcgccgctaa caacggtttc gactttcata
      961 tcatcgacaa tgaaatctat tgggacgaga gtcgggctta ccgttttact ctgcgccaga
     1021 ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca gatgtgcctt gaggtggtgg
     1081 atcgcgcggt taaagacgaa gagatcctga cgcaactggc gatcccgccg ttgtactggg
     1141 atgtgatcgc cgaaagctgg cgcgcacgcg atccttcgct gtatggccgg atggattttg
     1201 cctggtgtgg caatgcgccg gtgaagctgc tggagtacaa cgccgatacg ccgacctcgt
     1261 tgtatgagtc ggcttatttc cagtggttgt ggctggagga tgcccggcgc agcggcatta
     1321 ttccgcgtga tgccgatcag tacaacgcca ttcaggaacg cctgatttcg cgctttagtg
     1381 agctttacag tcgggaaccg ttttattttt gctgctgtca ggacaccgat gaagacagga
     1441 gtaccgtgct gtacttgcag gactgcgccc agcaggcagg gcaggagtcg cggtttatct
     1501 acattgaaga tctcggtttg ggcgtcggcg gcgtactgac cgatcttgat gataatgtca
     1561 tccagcgtgc atttaagctg tatccgctgg agtggatgat gcgtgacgat aacggtccgc
     1621 tgctgcgcaa gcgtcgcgag caatgggtgg agccgttatg gaaaagtatc ttgagtaata
     1681 aagggctaat gccgctgctt tggcgcttct tccctggtca tcctaacctt cttgcatcct
     1741 ggtttgaggg tgaaaaatcg cagattgccg ctggcgaaag ctatgtgcgt aaaccgatct
     1801 actcgcgcga aggcggaaac gtcaccattt tcgacggtca gaataacgtc gttgaccacg
     1861 ctgatggtga ttacgccgat gaaccgatga tctaccaggc gtttcaacct ctgccgcgat
     1921 ttggcgatag ctacacactc atcggtagct ggattgtcga tgatgaagcg tgcggaatgg
     1981 ggatccgtga agataacaca ctgatcacca aagacacctc acgtttcgtt ccgcattaca
     2041 ttgctggata agaatgtttt agcaatctct ttctgtcatg aatccatggc agtgaccata
     2101 ctaatggtga ctgccattga tggagggaga cacagtgcac tggcaaactc acaccatttt
     2161 taatcaacct ataccattaa ataacagcaa cttatacctg tctgatggcg cgctttgcga
     2221 agcggtaacg cgtgaaggtg ctggctggga tagcaatttt ctagccagta ttggtcagca
     2281 gttaggaacg gctgaatccc ttgaactggg gcggctggcg aatgtaaacc cgcctgaact
     2341 gttgcgctac gatgcccagg ggcggcgtct ggacgatgtt cgtttccatc ccgcatggca
     2401 cctgctgatg caggcgttat gtaccaatcg ggtgcacaat cttgcctggg aagaagacgc
     2461 tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg ttacatggca ggttgaggct
     2521 gggtcgttat gtccaataac gatgaccttt gccgccacgc cattgttgtt acagatgtta
     2581 cccacgccgt ttcaggactg gaccacgccg ctgctgagcg atcgctacga ttctcactta
     2641 ttgccaggtg ggcaaaaacg cggtttgttg attggcatgg gaatgacgga aaagcagggc
     2701 ggttccgatg tcatgagcaa caccatccgt gcagagcgtt tggaagatgg ctcttatcgg
     2761 ctggtggggc ataaatggtt tttctcggtg ccgcaaagcg atgcgcatct ggtgctggcg
     2821 cagaccgcgg gcggtctgtc ctgctttttt gtgccgcgct ttttgcctga cgggcaacgc
     2881 aacgcgattc gcctcgagcg gctgaaagat aagctgggta atcgctctaa cgccagttgc
     2941 gaagtggagt ttcaggatgc cattggctgg ttattggggc aggaagggga aggaattcgt
     3001 ctgatcctga aaatgggggg gatgacgcgt tttgattgcg ccctgggtag ccatgccatg
     3061 atgcgccgtg cattttcgct ggcgatttat catgcacatc aacgccatgt ttttggtaat
     3121 ccattgatcc aacagcccct tatgcgtcat gtcttaagtc gcatggcact tcagcttgaa
     3181 gggcaaacgg cgttgctgtt tcgtcttgcg cgagcgtggg accggcgtgc cgatgccaaa
     3241 gaagccctgt gggcgcgttt atttacgcct gcggcaaaat ttgtgatctg caaacgaggt
     3301 atgccgtttg tggccgaagc gatggaggtg ctgggcggca ttggttattg cgaggagagc
     3361 gagctgccgc ggctttaccg ggagatgccg gtaaacagta tttgggaagg ttccggcaat
     3421 attatgtgcc tggatgtgtt gcgcgttctc aataagcaag cgggcgtata cgacttattg
     3481 tcggaagcat ttgtggaagt gaaagggcag gatcgctatt ttgatcgcgc ggttcgtcgt
     3541 ttacagcagc agctgcgtaa gccagctgaa gaactggggc gagagattac tcatcagcta
     3601 ttcctgctgg gctgcggtgc gcaaatgttg aaatatgctt ctccgccaat ggcgcaggcg
     3661 tggtgtcagg tgatgttaga tacgcgcggc ggcgtacggt tgtcagagca gatccagaat
     3721 gatttattgc tgcgggcgac ggggggagtg tgtgtgtaag cgtatacgac tgatgcgacg
     3781 ctggtttcga ttaactaaat gaaatatgtg aaaattgtag gccggacaag gcgctcgcgc
     3841 cgcatccggc attgttcatt tgctgtagat gacactcatg catacagtat cgcctgtacg
     3901 cgccagttat caggctgctg gtcttcgtac atggtaataa tgcggtacca tgacgcacct
     3961 tgttcatcgg cctttaacgc cacgatacgt tccacgtcct gcggatcccc tgaaatattg
     4021 ctaactgaga tctgacctat ttcattcagg cccgtcacgc agtcagcact ggcgaattct
     4081 gcagactgtg ccgtggcgct cgcaggataa gtcactaatt gcagcgaggg tgaggcaagt
     4141 aattgtttca tcgtcagctc catttttcgt ttccccacat gttgcagggc attccatcgc
     4201 ggtaataaac gcccaagctt ccagccggta atttattgtg cacaggtaaa cgcataggga
     4261 cgcaaagcaa tgtaaagctg actatgtaag ttgtctgatt tatttacggt acaaaatggc
     4321 ttgtgaatac cactgtcctg tcacgatggt ttcgtccacc atgacgacaa cgtaataatc
     4381 agcttttgca gcgacagctt ttgctctgat ttctgctaat gcgtcatccg gagaaccccg
     4441 aaccatcgtg cttacgctac ctattcgctg taacccttgc gtctggtcgc ggcgaatctc
     4501 ttgcggatgg tccgttactg gcggtgctgg ctgtggcgta ccttgcagtg cgctacaggc
     4561 acttaacatc aacaccaata ataaactggc aaaccggtaa ataacgctat tacgtttcct
     4621 gctaaccata gtgtagtgcc ttattaattt aactttgggg gaatgttccc gaattgttac
     4681 ctggtacgcg attttcgaat gaaaacgtga cgaaagcgta acccacatct caacattatg
     4741 aactaatacc gccagcagaa acttgtgcta aggatttctt catgtcatac cagacaaaaa
     4801 ggagagacag atgattgaaa tagaatcacg cgagctggca gatattcccg ttcttcatgc
     4861 ttatcctgtc gggcaaaaag ataccccgtt accgtgcgta attttttatc acggctttac
     4921 ttcatccagc ctggtgtata gctattttgc cgttgcgctg gcgcaggctg gtttgcgggt
     4981 gatcatgccg gatgcgcccg atcacggtag ccgttttagt ggcgacgaag cgcggcggtt
     5041 aaatcaattc tggcaaatct tgctacaaag tatgcaggaa ttcactactt tacgtgcggc
     5101 aatagccgaa gaaaactggc tgcttgatga ccgtctggca gtcggtggcg cgtcgatggg
     5161 cgcgatgacg gcactgggaa ttaccgctcg ccaccccacg gtgaaatgta ccgccagcat
     5221 gatgggatcg ggctatttta catcgctcgc ccgttcactg tttccaccgc tgatacctga
     5281 aacggcagca cagcagaatg aattcaataa aattgtcgcg ccgctggcag agtgggaagc
     5341 gacaaaccac ctagaacaac ttggggacag acctctactg ctgtggcatg gcctcgacga
     5401 tgacgttgtg cctgccgacg aatcactacg tttgcagcag gccttaagcg agacgggacg
     5461 ggataaactg ctaacctgtt catggcagcc aggcgtgcgt caccgcatta cgcctgaggc
     5521 gttagatgct gccgtgacat ttttccgcca gcatctttaa acacgcagaa tgctgacccc
     5581 ttgcgcttcc agttgttgca ggatttccgg gttagcattt ttgccggtga tcagcatatc
     5641 gatttgatcg gcacggctaa aaagcattcc cgcgcgttcg ccaatcttac tgctatcaac
     5701 cagtaccacc agtttcccta ccacgctcag catcttctgc tctgccattg ctgtcagcat
     5761 atcggtttta tacaaccctt ctgcggtcag cccttttccg ctggtaaaca tccagtgccc
     5821 ggcatagaga ctgttttcgc tgccctgcgg gcttaaagtg atggactgac ttttgttgta
     5881 ctgtccaccc ataatgatca cgctgtcatg ttcttgatcg atcaggtaat ttgccagcgg
     5941 tagataatta gtgatgattt gcactggctt gccacacatt tcccgcccaa gcagaaacgc
     6001 ggtggagccg cagttgatga ctacgctttc gcccggatta accagctgcg aggccgcttt
     6061 agcaatacgt actttttcat cgtgattctg cgcctgatgc agattcatcg gcgtccagcg
     6121 cgggcgctgt tgggtaatag cttctgcgcc attgcgcacc tttttcagtt tgccgctttc
     6181 gccaagttta ttgatatcgc gtcgcgcagt ggcaggcgaa attcccagac gctcaacgac
     6241 tttctcaacg gtcacaaagc ccaattgtgc gagcatttcc aggaggattt gatgtctttg
     6301 tgcttcagtc atgagctatt ccgataaaat ttgatttttt tagatgatat ttgaaatagc
     6361 caggaaatac tacgccggat agcgcgaact tctccacgct atccggtata aacgacagat
     6421 tacaggaatg acttgaacgg cagatccggt tcaatagtga agcaatcatc gaaaccgcgc
     6481 ggatagtggt actcgaagtt gtctttatcc agcggccagg taaatttgcc gccaacctgc
     6541 cagataaacg gcttgaagcc atacttcaga cgatcttttt tcatctccca cagcacgcgg
     6601 atctcttgcg gatcggcctg gaagtttgac cagatgtcgt ggtggaacgg gataaccact
     6661 ttcgcattca gggcttcacc catacgcagc atatcggcgc tggtcatttt gtcggtaata
     6721 ccgcgcgggt tctcaccata cgatcctaac gccacatcga tctgatgttc gttaccgtgc
     6781 ttcgcataat agttagagta gtgggaatcg ccactgtgat atagggaacc gccaggggtt
     6841 ttgaacaggt agtttactgc gcgatcgtcc atgccatctg gcagtacgcc agccgctttt
     6901 tgatcggcag gcagggtgat cagtgcagta cggtcgaaag cgtcaagcgc atgaatttca
     6961 atgtctttca cttttacaac atcgcccggt ttgaccacga tgcaacgctc tttcggtacg
     7021 ccccagccaa tccacaaatc aacgcaggtt ttcggtccaa tgaatggtac gtcatcagca
     7081 cagttctgca tcacggcagc agcgacgtta acgtcgatat gatcgttgtg atcgtgagtc
     7141 gccagtaccg cgtcgatctg gcgaatcgca aacggatcaa gaacaaacgg ggtggtgcgt
     7201 aggtttggct gcagtttttt cacgccagcc atgcgctgca tctggtgacc ctgtttcatt
     7261 aacgggttac cgtgactttg tttgccagtg ccgcaccaga aatcaacgca aacgttggtg
     7321 ccaccttctg atttcaacca gatcccggtg cagcctagcc accacatcgc aaatgtgcca
     7381 ggggcgacct gctcttgttc aatttcttca ttcagccagc taccccactc cgggaaagtg
     7441 ctcaggatcc aggattctcg ggtgatactt ttcactttac tcatcgccat ttaccttcat
     7501 gatagttcaa ttcgaatcaa tatgtgattg gttttgatta atcctgacac tattttttca
     7561 ggaaggcaat gaccattttt tgactttttg ccagggaagt tgttgttgat tttgagtgta
     7621 gaaagattta atggaatgta tagttaaatt aactaaatga atttaaataa ataatttttt
     7681 cgttgtgtga atcccactct atccgtgtgg aattatttgc gggtcgcgtc acatttaatc
     7741 ataaataatc ttgttgtgat tacttttgaa aattagagtg agtgcacaac attccgggtg
     7801 tgcggaatac ccggttacct cttcttcagg agatcgttat ggagatcctc tacaacatct
     7861 ttaccgtgtt ttttaaccag gtcatgacca atgccccgtt gttgctgggt attgtgacct
     7921 gtctgggcta catcctactg cgcaaaagtg tcagcgttat tattaaaggc acgattaaaa
     7981 ccataattgg tttcatgttg ttgcaggcag ggtccggcat cctcaccagc accttcaaac
     8041 cggtggtggc gaaaatgtcc gaagtctacg gcattaacgg cgcaatttcc gatacttacg
     8101 cgtcgatgat ggcaaccatc gaccgcatgg gcgatgccta tagctgggta ggttacgcgg
     8161 tgttgttagc gctggcgctg aacatctgtt acgtgctgct gcgtcgcatt accggtattc
     8221 gtaccatcat gctgactggt catatcatgt tccagcaggc ggggctgatt gccgttacgt
     8281 tgtttatttt cggctactcc atgtggacaa ccattatctg cacggcgatt ctggtttcgc
     8341 tctactgggg cattacctcc aacatgatgt acaagccgac tcaggaagtg acggacggct
     8401 gcggtttctc catcggtcac cagcaacagt ttgcatcatt aattgcctat aaagttgcgc
     8461 cgttcctcgg caaaaaagag gaaagcgttg aagacctcaa actgccaggc tggctgaata
     8521 ttttccacga caacatcgtc tccacggcga ttgtcatgac catcttcttt ggtgccattc
     8581 tgctctcctt cggtatcgac accgtgcagg cgatggcagg caaagtgcac tggacggtct
     8641 atatcctgca aaccggtttt tccttcgcgg tggcgatctt catcatcact cagggtgtgc
     8701 gcatgtttgt ggcggaactc tctgaagcgt ttaacggtat ctcccagcgc ctgatccctg
     8761 gtgcggttct ggcgattgac tgtgcggcta tttatagctt cgctccgaac gccgtggtct
     8821 ggggctttat gtggggcacc atcggtcagc tgattgcggt tggcatcctg gtcgcctgcg
     8881 gttcctcgat cctgattatc ccaggcttta tcccgatgtt cttctccaac gccaccatcg
     8941 gcgtgttcgc taaccacttc ggcggctggc gtgcggcgct gaagatttgt ctggtgatgg
     9001 ggatgatcga aatctttggt tgcgtctggg tggtgaaact taccggtatg agtgcctgga
     9061 tggggatggc ggactggtcg attctggcac cgccgatgat gcagggcttc ttctccatcg
     9121 gtatcgcctt tatggccgtc atcattgtaa ttgcactggc ttatatgttc ttcgctggcc
     9181 gcgcactgcg cgcagaagaa gatgcagaaa aacaattggc agaacagtct gcttaataag
     9241 gagttttgat tatgaccgta cgtattctgg ctgtgtgtgg caacggacaa ggcagttcca
     9301 tgatcatgaa gatgaaagtg gaccagtttt taacccaatc aaacattgac cacacggtaa
     9361 atagctgcgc ggttggcgaa tacaaaagcg agttgagcgg cgcggatatc atcatcgctt
     9421 ctacgcacat tgcgggcgaa atcaccgtga ccggcaacaa atacgtggtt ggcgtgcgca
     9481 acatgctctc tcctgccgac tttggcccga aactgctgga agtgatcaaa gcgcatttcc
     9541 cgcaggatgt gaagtaagga cgcgccatga aattacatga ttcgctggcg gagaataaat
     9601 ccatccgcct gcaggctgaa gctgagacat ggcaggacgc agtaaaaatc ggcgttgacc
     9661 tgctggtggc ggcggatgtt gtcgagccgc gttactacca ggcgattctg gatgcggttg
     9721 aacagcacgg tccgtacttt gtgctcgcac caggcctggc aatgccgcac ggtcgcccgg
     9781 aagagggcgt taagaaaacc ggttttgcgc tggtgacgct gaaaaagccg ctggagttca
     9841 accacgaaga taacgacccg gtggatatcc tcatcaccat ggcggtggtc gatgccaaca
     9901 cgcactagga agtgggcatc atgcagatcg tcaacctgtt tgaagatgaa gaaaatttcg
     9961 accgtttacg tgcctgccgt accgagcagg aagtactgga tctcattgac cgcaccaacg
    10021 cggcagctta agaaggaatt gaagatgtca ttaccgatgt tgcaagtcgc gctggacaac
    10081 cagactatgg atagcgccta cgaaaccact cgcctgattg ccgaagaagt cgacattatc
    10141 gaagtgggta ccattctgtg cgtgggcgaa ggcgtgcgtg cggttcgcga tctgaaagcg
    10201 ctctacccgc acaaaatcgt gctggcagac gccaaaattg ccgatgcggg caaaattctt
    10261 tctcgtatgt gcttcgaagc caacgctgac tgggtgaccg tgatttgttg tgcggatatc
    10321 aacaccgcca aaggtgcgct ggacgtagca aaagagttta acggcgacgt gcagatcgaa
    10381 ctgaccggtt actggacctg ggaacaggcg caacagtggc gcgacgcagg tattcagcag
    10441 gtggtttatc accgcagccg tgacgcgcag gccgctggcg tggcgtgggg tgaagccgat
    10501 atcactgcga tcaaacgtct ttccgatatg ggcttcaaag tcaccgtcac cggtggcctg
    10561 gcgctggaag atctgccgct gttcaaaggc attccaattc atgtgtttat cgccggtcgc
    10621 agcatccgtg atgccgcttc tccggttgaa gccgcgcgtc agttcaaacg ttccatcgcc
    10681 gaactgtggg gctaaggagc ggatatgttg tccaaacaaa tcccgcttgg catctatgaa
    10741 aaagcgctcc ccgccgggga gtgctggctg gaacgcctgc gactggcaaa aacgttgggc
    10801 ttcgattttg tcgagatgtc ggtagatgaa actgacgagc gcctgtcgcg cctcgactgg
    10861 agccgcgagc agcgtctggc gctggtgaat gcgattgttg aaaccggcgt gcgtgtgccg
    10921 tccatgtgcc tttctgccca tcgtcgtttc ccgttgggca gcgaagatga cgcggtgcgg
    10981 gcgcaggggc tggagattat gcgtaaagcc atccagttcg cccaggacgt tggcattcgc
    11041 gtgatccagc tggcgggcta tgacgtttac tatcaggaag ccaataacga aacgcgtcgt
    11101 cgtttccgtg acggcctgaa agagagcgtt gagatggcga gccgcgcaca ggtgacgctg
    11161 gcgatggaga tcatggatta tccgttgatg aactccatca gcaaggcgct gggttacgcg
    11221 cactatctca acaacccgtg gttccagctc tacccggata tcggcaacct gtcggcgtgg
    11281 gacaacgatg tgcagatgga gttgcaggcc ggaatcgggc atatcgtcgc ggtgcatgtg
    11341 aaagacacca aaccgggcgt cttcaaaaac gtgccatttg gcgaaggtgt ggtggatttc
    11401 gaacgttgct tcgaaacgct caaacagagt ggctattgcg ggccgtacct gattgagatg
    11461 tggagcgaaa cggcggaaga cccggcggca gaagtggtga aagcgcgcat ggccaaagca
    11521 ggcatggtgg aggcggcata atgcaaaagc taaaacagca ggtgtttgaa gccaacatgg
    11581 atctgccgcg ctatgggctg gtgaccttta cttggggcaa cgtcagcgct atcgaccgcg
    11641 aacgcgggct ggtggtgatc aagcccagcg gcgttgccta cgaaaccatg aaagcagacg
    11701 atatggtggt ggtcgatatg agcggcaagg tggtggaagg ggagtatcgc ccatcttccg
    11761 acactgcgac gcatctcgaa ctctaccgtc gttacccgtc gcttggcggc attgtccata
    11821 cccactccac tcatgccacc gcatgggcgc aggcggggct ggcgatcccg gcattaggca
    11881 ccacgcacgc tgactacttc tttggtgata tcccgtgtac gcgtggatta agcgaagaag
    11941 aggtgcaggg tgagtatgaa ctgaacactg gcaaagtgat tatcgaaacg ctgggcaacg
    12001 ccgagccgct gcatacgccg ggaattgtgg tgtatcagca cggaccgttc gcctggggga
    12061 aagatgccca cgacgcggtg cataacgcgg tagtgatgga agaagtggcg aaaatggcgt
    12121 ggattgcgcg cagtattaat ccacaactca atcatatcga cagctacctg atgaataaac
    12181 acttcatgcg taagcacggt cctaacgctt attacgggca gaagtaaaac acgcgctgcg
    12241 gaaatttcct tcctcgggag ataactggtc taattccgca gccgtttttc aaaaaaagcc
    12301 ccctgcgaag ggggcaaagc aaactatggc aatgtttcgt tggttatacc tggtgctagc
    12361 gataaatatc cgcgctggcg tgcatattgc cgttactccc cggttcccgc atcagaatta
    12421 cgtggtagta cgttgcgcct tgcgcatctg tttcttcatt taatgcctga cgtgcttcgc
    12481 tttcagtggc gaaattatga ttgatataaa tcacgcctaa gctttgcaca tcgtccatat
    12541 ttctggcctg gtggttatta atttcaatgg ctgcccatgt atttgcactt agcaaaagca
    12601 cagccagaag ggctaaaact cgactgaaca tagatacctc ctcgacggct gactttgtgt
    12661 gctctcctct gtgatgatct tctgatttaa ttttaatcaa tgataaagaa gttgatggtg
    12721 accatttctg atgcagttgt tcaaaaaaac actgtgacgg aatgtgatga acttcaaatc
    12781 agcggtttag aggttatttg cgaaagggga gatttatttc tgctccgccc ttgagtttag
    12841 cggggtatgt cagtactata acgcgtcatt tttcagccga cctttaacac gttccttgcc
    12901 tccccgggat tcggctgacc cagacaggag gctgaataat ccgtaaggag caattcgatg
    12961 cgtcattacg aaatcgtttt tatggtccat cctgaccaga gcgaacaggt tccgggcatg
    13021 atcgagcgct acactgctgc catcactggt gcagaaggca agatccaccg tctggaagac
    13081 tggggccgcc gtcagctggc ttacccgatc aacaaactgc acaaagcaca ctacgttctg
    13141 atgaacattg aagctccgca ggaagtgatc gatgagctgg aaactacctt ccgcttcaac
    13201 gatgccgtta tccgcagcat ggttatgcgt accaagcacg ctgttaccga agcatctccg
    13261 atggttaaag cgaaagacga gcgccgtgag cgtcgcgatg atttcgcaaa cgaaaccgct
    13321 gatgatgctg aagctgggga ttctgaagag taatttctga tgaccaaccg tctggtgttg
    13381 tccggcaccg tgtgcaggac tccccttcga aaggtcagtc catcaggaat tcctcactgc
    13441 cagttcgtgc ttgagcatcg ttctgtgcag gaggaagccg gctttcaccg gcaggcatgg
    13501 tgtcaaatgc ccgttattgt tagcggacac gaaaaccagg ccattactca cagtataacg
    13561 gtcggcagtc gcataaccgt tcaggggttc atttcatgcc acaaggcaaa gaacggactg
    13621 agcaaaatgg ttttgcacgc cgagcagatt gaattgatag attctggaga ctagccatat
    13681 ggcacgttat ttccgtcgtc gcaagttctg ccgtttcacc gcggaaggcg ttcaagagat
    13741 cgactataaa gatatcgcta cgctgaaaaa ctacatcacc gaaagcggta agattgtccc
    13801 aagccgtatc accggtaccc gtgcaaaata ccagcgtcag ctggctcgcg ctatcaaacg
    13861 cgctcgctac ctgtccctgc tgccgtacac tgatcgccat cagtaatcgg tcacggtcca
    13921 ttaatacgac tttgagagga taaggtaatg caagttattc tgcttgataa agtagcaaac
    13981 ctgggtagcc tgggtgatca ggtaaacgtt aaagcgggct atgctcgtaa cttcctggta
    14041 ccgcagggta aagctgttcc agctaccaag aaaaacattg aattcttcga agcacgtcgc
    14101 gctgaactgg aagctaaact ggctgaagtt ctggcagctg ctaatgctcg cgctgagaaa
    14161 atcaatgcac tggaaactgt taccatcgcg tctaaagctg gcgacgaagg taaactgttc
    14221 ggttccatcg gtactcgcga catcgctgac gctgtaactg cagctggcgt tgaagtggct
    14281 aagagcgaag ttcgtctgcc gaacggcgtt ctgcgtacca ctggcgaaca cgaagtgagc
    14341 ttccaggttc acagcgaagt attcgcgaaa gtgatcgtaa acgtagtagc tgaataattc
    14401 gttattcaac gagacgtaaa aacgccgacc attggtcggc gttttgcttt ctatttttcg
    14461 tcaggcatta atttcgcaag tagatccatt caccattctc ttgtgtccat aagtcaggat
    14521 ggtttgctgg cgttagatgc atacgatcac tccttatgtc tcggtcctgc ggcactcgct
    14581 cgaaacctaa acccgagtag attgcggctg aatttgataa ggaacgtaga ccaattttca
    14641 aatcatgggg taaatcagaa tacgcgttta gcaatgcagc aatgatgaat ccgagggcat
    14701 tagaaacgcc tgtaacagtg cgaagttggt cgatataaag atcgattgtt ggtagttcag
    14761 atgaccagca attaggcatt tgggcaggaa gggttacaac gtatcctatc gtttggccgg
    14821 tatagtctgg agtactataa ccaggtccca aaacaacctc gtgtattgtg tatttactgt
    14881 tgggttcgaa ggattcgaca tcttgcaata aatatttcag ttctgcggtt aacctcgcac
    14941 ccgcgtaagc tttattgtat ttctgggcgt gaatattatc taatgtagct tcatggttcc
    15001 atccaaaaga attatcacca ttcattgt
//
LOCUS       DAJVQH010000099        14945 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJVQH010000099 DAJVQH010000000
VERSION     DAJVQH010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.099"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..347
                     /locus_tag="OQM87_003032"
     CDS             <1..347
                     /locus_tag="OQM87_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HCS1509091.1"
                     /translation="QVFFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPASL
                     LTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSQLRDLVKTGATRKI
                     RSEARHRLEEKQ"
     gene            344..799
                     /locus_tag="OQM87_003033"
     CDS             344..799
                     /locus_tag="OQM87_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509092.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(877..1788)
                     /gene="rdgC"
                     /locus_tag="OQM87_003034"
     CDS             complement(877..1788)
                     /gene="rdgC"
                     /locus_tag="OQM87_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCS1509093.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1913..2821
                     /gene="mak"
                     /locus_tag="OQM87_003035"
     CDS             1913..2821
                     /gene="mak"
                     /locus_tag="OQM87_003035"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCS1509094.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            complement(2964..4145)
                     /gene="araJ"
                     /locus_tag="OQM87_003036"
     CDS             complement(2964..4145)
                     /gene="araJ"
                     /locus_tag="OQM87_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCS1509095.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMILTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(4271..7414)
                     /gene="sbcC"
                     /locus_tag="OQM87_003037"
     CDS             complement(4271..7414)
                     /gene="sbcC"
                     /locus_tag="OQM87_003037"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCS1509096.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(7411..8613)
                     /gene="sbcD"
                     /locus_tag="OQM87_003038"
     CDS             complement(7411..8613)
                     /gene="sbcD"
                     /locus_tag="OQM87_003038"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCS1509097.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8803..9492
                     /gene="phoB"
                     /locus_tag="OQM87_003039"
     CDS             8803..9492
                     /gene="phoB"
                     /locus_tag="OQM87_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCS1509098.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            9550..10845
                     /gene="phoR"
                     /locus_tag="OQM87_003040"
     CDS             9550..10845
                     /gene="phoR"
                     /locus_tag="OQM87_003040"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCS1509099.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            11252..12571
                     /gene="brnQ"
                     /locus_tag="OQM87_003041"
     CDS             11252..12571
                     /gene="brnQ"
                     /locus_tag="OQM87_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCS1509100.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            12647..14020
                     /gene="proY"
                     /locus_tag="OQM87_003042"
     CDS             12647..14020
                     /gene="proY"
                     /locus_tag="OQM87_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCS1509101.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            14176..>14945
                     /locus_tag="OQM87_003043"
     CDS             14176..>14945
                     /locus_tag="OQM87_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HCS1509102.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDS"
BASE COUNT         3316 a         3799 c         4022 g         3808 t
ORIGIN      
        1 tccaggtatt ttttgcggaa ctggctacga taaaagataa atcagatgat gaatggtggc
       61 gtgcattgct gcaaagtcgc aatgatcatt tgcgccaaac ggcattacgc aacgcacata
      121 ccccggcgtc gttgttaacg acattgactg agcctcagga tcggtcgctg gctattaata
      181 atccacagct ggctgccgat gtgaaaacgg cgtggttaaa agaggatcca tcattactct
      241 tatttgtcga acaacccgat ctttcgcagt tacgtgattt agtgaaaacc ggggcaacgc
      301 ggaaaattcg cagtgaagcg cgtcaccggc ttgaggaaaa acaatgacac aacgtccatg
      361 gagcaagtta cagcgggaga tctatgactt actgacgcca acgatcaacc tgcaaattca
      421 ctgtacccgt tatcccatgc gcagccaaaa tggtggaagt accgatttac cgcgttattg
      481 gatcacgttg gataaaaacg tgatctggga ttacccgaaa gactttattg caggtaacgc
      541 tggagtgcgt aattttcacg gtgaaacttg ctggtatcct tacctgacgg atatttgttc
      601 aatttctgat ttattgcgtg aatatattga taccccaaaa gcggagctgt taacaaaaca
      661 atttaccagc gataaatggg ggttagtgaa tattctgcgt gctgctgaca ggcgaatagg
      721 gatgaggcga ttagatcagt tacggcgtaa aacgcataat attgctgcgc taaaaattat
      781 tgctcgccgt agcgaataat tatatgcctg gtgtggcttc gtacgccgga taagacgcgg
      841 caggcgtcgc atccggcatt aaaggaaaat cagcaattaa cgttgtgctt cgccacctaa
      901 tccttcaatc aggttttgaa ttaacgctgc cagttcacca gtcatcagga tgaaatcggc
      961 atcaaaacgc tgggcgaaat cttcacggtc gatatcttcg ttttgatcgt gcagctcgtc
     1021 gcagaactta agacgcttga gcgaaccatc gtcgcacatc acaaactgaa tgcgctgctg
     1081 ccagtcgagc gccagtttag tcaccacttt tccggcttca atgtgattgg tgatctcttc
     1141 gctggtcaga tcttgtttct tcgcgcggat cacgccgcca tcttccagca acgatttcag
     1201 ctcggcttca tcaagcagct ggaagccctg tgccgcacta ccggagcgaa cccattcggt
     1261 cagcgtcagt tcaatcgggt tttccatgct caatggtaca accggtaacg accccaggct
     1321 tttacgcagt aatgccagcg tatcttccgc ttttttggca ctggcgcagt ccaccataat
     1381 caaaccgtta accgtgtcga gccacatcat tgtctggcta aaacggctga aagcacgcgg
     1441 cagcagagag tgcagcactt cgtctttcag cgaatctttt tcggttttct tcagcttacg
     1501 cgcctgttcc gcttccagtt tggcgatttt cgcttccagc gcctgtttaa tcaccggaga
     1561 cgggaggatt ttttcttctt tgcgcgcgca gataacaatt tgaccattgg caacgtgcgt
     1621 taacgcatcg ctgtgcgatc ccatcggagg aacccagccc atcttcgcca tgtcctggct
     1681 gccgcatggg gtaaatgcca tcgaggctag ctgtttttcc atctcttctg cacgcagcga
     1741 aatctcgcgg ctaagacggt aaaccattaa atttttgaac cacagcatga taatttccac
     1801 ggccttgtcg ttaaatttag cgggcatgat aacgaattgt cggcggcctt gcattgccaa
     1861 tccggttgtc cgtctctacg ctattgatat tgaaaaaaat aaggagagta cagtgcgtat
     1921 aggtatcgat ttaggcggca ccaaaactga agtgattgca ctgggcgatg caggggagca
     1981 gttgtaccgc catcgtctgc ccacgccgcg tgatgattac cggcagacta ttgaaacgat
     2041 cgccacgttg gttgatatgg cggagcaggc gacagggcag cgcggaacgg taggtatggg
     2101 cattccaggt tcgatttcgc cctacaccgg tgtggtgaag aatgccaatt caacctggct
     2161 caacggtcag ccattcgata aagacttaag cgcgaggttg cagcgggaag tgcggctggc
     2221 aaatgatgcc aactgtctgg cggtttcaga agcagtagat ggcgcggcag cgggagcgca
     2281 gacggtattt gccgtgatta tcggcacggg atgcggcgcg ggcgtggcat tcaatgggcg
     2341 gacgcatatc ggcggcaatg gcacggcagg tgagtgggga cacaatccgc taccgtggat
     2401 ggacgaagac gaactgcgtt atcgcgagga agtcccttgt tattgcggta aacaaggttg
     2461 tattgaaacc tttatttcgg gcacgggatt cgcgacggat tatcgtcgtt tgagcggaca
     2521 tgcgctgaaa ggcagtgaaa ttatccgcct ggttgaagaa agcgatccgg tagcggaact
     2581 ggcattgcgt cgctacgagc tgcggctggc aaaatcgctg gcacatgtcg tgaatattct
     2641 cgatccggat gtgattgtcc tggggggcgg gatgagcaat gtagaccgtt tatatcaaac
     2701 ggttgggcag ttgattaaac aatttgtctt cggcggcgaa tgtgaaacgc cggtgagtaa
     2761 ggcgaagcac ggtgattcca gcggcgtacg cggcgctgcg tggttatggc cacaagagta
     2821 aaaaacgtag gcaattggcg catcatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     2881 tacaaaatgt gccagaaccg cgtagggcgg ataaggcgtt cacgccgcat ccggcaataa
     2941 gcaacgcgca ccgtgacagt aatctacccc agtggtttcg ccagcaccgg actatccgcc
     3001 gcttgctggc gcttatagcg accatacagc agcaacgatg acatcgcagc aaacgaaaac
     3061 agggcggcag gcagtgccac gtaattatat gccagcccca gcgtcagtat catacctccg
     3121 caatatgcgc cgacggcgct accgaggtta aacgctattt gcccacctgc ggcacctaat
     3181 aactctccgc ctttggcatt ttgcagtaac aatatttgca gaggtgctga aagggcaaat
     3241 aatcccgcgc aacaaataaa agcaaaaata agcgacgttg ttttcatgcc gccgcagaaa
     3301 aagagcatca gcagtgccag tacaatgata aagtcagtca ctgctgcaat gcgcagtggt
     3361 gaataacgtc ctgaaatcct gccacttagc atatttccca gcaccatccc tagcccaact
     3421 aacatcataa taaaggtcat cgccgtttcc gaaaaaccgg aaataaacat catgtatggc
     3481 tttacgtagc tgaaccaggc aaacacacct gcgttgccaa acatcgtggc ggcgaaaatt
     3541 aaccacgggg ccgggctgcg caaaaagtga aattgttcgc gcagatttcc tttcgcctcg
     3601 tcgcgaatat ctggcaccca aaaatagacc gatgccatca ccgcaatatt aaaaacagcg
     3661 atcaataaaa aggtgtaacg ccagcattcc tgacttaaat acgttcccag cggaatgccc
     3721 agcaaattgg cgactgtcat cccggaaacc atccccgcca cggcggcggt gacttttccg
     3781 ggtttgataa tttttgataa cacgatcgct ccgacgccaa aaaatgcgcc atgcggaaag
     3841 ccggatacca gccgaccaat ggcgagcatc aggtaagacg aagagagcgt gaacatggcg
     3901 ttgccaatga cgcacaacgc caccagaaac aacaagatat gtttgagtga gtagcggctg
     3961 gaaaagagtg cgatgattgg cgcaccgacc accaccacca gtgcataata cgagatcata
     4021 tgcccggcag caggaatcga aattcctacg ttatgcgcca gctccgtgag cacgctcata
     4081 atgccaaatt cggctatccc caaaccaaac gtgcccagag ccaaagataa aatgactttt
     4141 ttcataccac catcacaacc accagaacgg ctaccaatca ccgtagccaa taaagtgata
     4201 gcttgcagga taacgaccag caaggccaag agataattcg cccctctgta ttcattatcc
     4261 tgctgaatag ttatttcact gcaaacgtac tttccagttt gctgtagccc aggccgttga
     4321 tctttttcac tttgatctgc accggaatac gctctttcat cgcttctacg tggctaatca
     4381 caccgatggt tttgccactg gcgttcaggg catccagcgc atcaagggcg gtatccagcg
     4441 tttcgctatc cagcgtgcca aaaccttcat caaggaacag cgagtcaata cgtgttttat
     4501 ggctgaccag atccgaaagc gccagcgcca gcgccagact aacgaggaaa ctttcgccgc
     4561 cggaaagggt acgggtatcg cgtaccgcat ctgcctgcca ggtatcaaca acctcgactt
     4621 ccagcgcctc gctggctttg cgctgtaaca gatagcgccc gtgcagccgg gtaagttgct
     4681 gattagcgag atggactaaa ttatccagcg tcagcccctg ggcaaacttg cggaatttat
     4741 cgccctcttt ggaacctatt agcgaattca gatatcccca gtcctcaacc tgctgcgtca
     4801 tttgagcaat ttgctgcatt aaggtttgtt gttgctgacg gttatctgca tcctgcttca
     4861 gctgctggcg aatctcgcct tgactcgtgg tgttttcacg caacttttgg tgagtttgcg
     4921 ctaactcttg ctgaatctgc tccaccgtca cagtgagagc caacccgtca ggtcggtgtt
     4981 gttgatgctg tgccagcgtt tctgctgtct gagtgaccag cgtttgcgcc tgacggcgct
     5041 ggttttccag attctgcttg agctgttcca gctgcgttag tgtttgttca tccattagcg
     5101 ccgcaaggaa cgcctgctga tcgtcaaaga cgctggcctg tagcgtggtg tcaaactgcg
     5161 cttgggcttt ttgcagactt tgcgccgcca gaacatcctg ttgctgtaac gtctgctgct
     5221 ggctgtgtaa tgcgagacat tgttcatgaa cctgccgcca gttatccagc gccacagttt
     5281 cttcgctgtg cgggagatca tcactttgcg gcaacgtttc cagaatcggc gtcagctgct
     5341 gaatacggtt ttgcagcgcg gttaattcgt tctggcgttg ctgccagctc tgcgcttctt
     5401 gctgacgtgt cgccaaccag ctctcttctt catcttcctg tggcaatgtc agtgcataac
     5461 ccgccaatgc cgttaagagc tgctgctgac gttgttcaat ttgctgttga tactggataa
     5521 tttgctgatt atgcgcggca atctgccctt gtaattcatg tcgttggctg agtaaccgca
     5581 gctggcgttc gtgctcatct tgtgcatcca gccacggttg aatatcgtcc tgtggctgca
     5641 aggtgatatt gaggctggcc gtgacggctt gccattgttg agtaagtgct tgctcatctt
     5701 gtcggaggct ttgcgcttcg ttttcatcgc gctgaagctg cttcgttaat gcatccagtt
     5761 gcccgcgcag cgccgcacct tcttcgccga gctttttaac ttcgttttcc agcgccagta
     5821 atcgagactg attaacacca ggctccagcg cctgatacgc ctcgaccgcc gggtggctgg
     5881 tggaaccaca aagtggacaa ggctgacccg cctgtaactg tgcacgctgg gcttccagcg
     5941 ttttgatgcg cgcttcctgc tcgcaaatgg ttttcacatc ggcaagttgc tgcgtctttt
     6001 ctttataacg ctggcgcatt tcgttaagtg cgacattacg ttgcgtctgt tccagagtga
     6061 cattctggat agtgaccatt aactgcgcca gacgtttttg ttggggaaca atctgcccat
     6121 gcagcgcgac caggcgctga cgcagtgggc gttgctcggc atgttgcgcc agggcggtag
     6181 caacttcatc ggcggttaac gtcaacgtga tcgccgcaag cgcattaagt ttttgctcag
     6241 catgggttaa ctgttgctgc cattgccgca gatgctcgcg atcgctggtt tgttgggaga
     6301 actgcgcacg ccaacccgcc agttcgttgt tccactgacg gaagcggtcg tgttcctgta
     6361 accaggtatt caggctttgt tgctgctgct gtaattctgc tgactgcttc gccgcgtggt
     6421 ggcgaatgct cgcgcgaagc gccattgtgc tctgtaagcg agtatttact tcttcaatct
     6481 gctggcgaat atgcgccagc gccgcgctgt gttctgcgat gcgttcccag tgtggacgaa
     6541 gatttcgtgc cggttgtgcc agactaagcg ccgccagttg aggttgcgct ttttcttctt
     6601 cggctaacgc ctgttgcaag gcctgctgac ggcggctggc ttcttgctgc aattcgtcct
     6661 gacgcgttaa ccagtttagc gattgttgtt cttgctgctg cgcggtaatt aactgttttt
     6721 cttcgtcagt aagtacctgc aaactcgctg tcagcgattg cacttgttcc ggcgtgagca
     6781 acgtgacgcc gctggcctgc gcttgcagct tctccagctc agtacgggcc gatttgtgct
     6841 gctcaaaaac catcgccgag atttgcccgt agatttcagt gccggttaac tcctcgagca
     6901 attccgcgcg ttctttgggt ttggcattca ggaaggcagc aaattgcccc tgcgaaagca
     6961 gcatcgaacg ggtgaagcgc ccgtaatcca gcccggttaa cgtcgctgtc agttccagct
     7021 tatctttcac tttgtcggcg agaattttgc cgtcggcgca gcgcgccagc tctacgcgag
     7081 gcacctgcaa attaccgtcc ggctggttac gcgcccgatt ctggctccag aacgcgcgat
     7141 acgcttcacc tttcacttca aactccacct ccgccagaca ttcagcggta tcgcgggtca
     7201 tgagatcatt ttgcgattgt gaaacgttag agagacgcgg agtttcgtga tacagcgcca
     7261 gacaaatggc gtccagcagg gtggttttcc ccgcaccggt tgggccggta atggcaaaca
     7321 gcccgttgct ggcgaacggc tcgcgggtga aatcaatctt ccattcgcct tttaatgagt
     7381 tcaggttttt caggcgcagg ctgagaattt tcatgcttcg tgttctccgg cgagggtatg
     7441 caacgtcgtg gtgaaaagat gctgcagacg ttgctgctgc gattcatcca gttcttccag
     7501 tgccagacgg cgattgaaca cctcttcgac gctgagttcg ctgagggttt cacgctgttg
     7561 gctggctaac acacgctcgc gctgttcacg actccgacgt accagcaata cttcgacagg
     7621 caatgattcg gttaatgcct ggattttgcg ctgaatatca tgcagatact catcagtagt
     7681 gatttcgata tccagccaga caggtggctc ctgcgataca tcgcgccact gttccagctg
     7741 tgcggtaatc gacgccagat cgcctttcag caccgccatg ggttgagtta ccggtacgtt
     7801 caggttttcc acgctctcta atttgccgtt tgaaaatgtc accagatgga catatttact
     7861 cttaccgcat tcatcaaaac tcagtggaat gggggaaccg caatagcgaa catgttccat
     7921 gccgccaata atctgtgcgc ggtgaatatg cccgagcgcg atgtagtcgg ctggtggaaa
     7981 gttttgtgcc ggaaacgcgt ccagcgtgcc aatataaatg tcacgcacgg cgtcactttt
     8041 actggcaccc acggtcgtta aatgtcccgt ggcgatgatg ggcagaggct gatcgccgcg
     8101 cagtttgcag gcatcggcat agtgttgttg gtaataatcg gtaatcgctg ccagtaaatg
     8161 ctgctgtttt tcaataccgt taagccccgc ctggctggaa ataatgtcac gcgggcgtaa
     8221 aaacggaatg gggcacagca ctgcgcctgg cgtcccgtcg cgacgaggca agatttgcgg
     8281 cgcatgtccg gcgctggcga ccacggttgt attgaggaac gccatgatat cgcgcgattc
     8341 attcagcgtg gcgaccgagt catggtttcc tgccagtacc accagatgac agccagtttg
     8401 ctgtaaattg acaacaaaac ggttgtataa cgtgcgggcg taactgggcg gcgagccggt
     8461 atcgaaaaca tcaccagcaa caataatcgc atccacctga tgggtttgtg ctgtctccag
     8521 cagccagtca agaaaagcct gatgttcagc ttcgcggctt ttactgtaga agttctggcc
     8581 gagatgccag tctgaggtgt gaaggatgcg cataacggtt ccctggcgaa aaagcatggt
     8641 cgcgattata cccaaacaga tgtgccattt gcttttttct gcgccacgga aatcaataac
     8701 ctgaagatat gtgcgacgag cttttcataa atctgtcata aatctgacgc ataatgacgt
     8761 cgcattaatg atcgcaacct atttattaca acagggcaaa tcatggcgag acgtattctg
     8821 gtcgtagaag atgaagctcc aattcgcgaa atggtctgct tcgtgctcga acaaaatggc
     8881 tttcagccgg tcgaagcgga agattatgac agtgctgtga atcaactgaa tgaaccctgg
     8941 ccggatttaa ttctccttga ctggatgtta cttggcggct ccggtatcca gttcatcaaa
     9001 cacctcaagc gcgagtcgat gacccgggat attccagtgg tgatgttgac cgccagaggg
     9061 gaagaagaag atcgcgtgcg cggccttgaa accggcgcgg atgactatat caccaagcca
     9121 ttttcgccga aggagctggt ggcgcgaatc aaagcggtaa tgcgccgtat ttcgccaatg
     9181 gcggtggaag aggtgattga gatgcaggga ttaagtctcg acccgacatc tcaccgagtg
     9241 atggcgggcg aagagccgct ggagatgggg ccgacagaat ttaaactgct gcactttttt
     9301 atgacgcatc ctgagcgcgt gtacagccgc gagcagctgt taaaccacgt ctggggaact
     9361 aacgtgtatg tggaagaccg cacggtcgat gtccacattc gtcgcctgcg taaagcactg
     9421 gagcccggcg ggcatgaccg catggtgcag accgtgcgcg gtacaggata tcgtttttca
     9481 acccgctttt aacgccttgc tcatcggacg cagagcaggg cttatgattt cttaactgga
     9541 gtatcttacg tgctggaacg gctgtcgtgg aaaaggctgg tgctggagct gctactttgc
     9601 tgcctcccgg ctttcatcct gggtgcattt tttggttacc tgccctggtt tttgctggca
     9661 tcggtaacag gactgcttat ctggcatttc tggaatttat tgcgcctttc atggtggctg
     9721 tgggtggatc gcagtatgac cccgccaccg gggcgtggta gctgggaacc gctactatac
     9781 ggcttacacc agatgcagct gcgaaataaa aaacgccgcc gtgaactggg caatctgatt
     9841 aaacgctttc gtagcggcgc ggagtcgctg cccgacgcgg tggtgctgac cacggaagag
     9901 ggcggtattt tctggtgtaa cggtctggcg caacaaattc ttggtttgcg ctggccggaa
     9961 gataacgggc agaacatcct taacctactg cgttacccgg agtttacgca atatctgaaa
    10021 acgcgtgatt tttctcgccc gctcaatctg gtgctcaaca ccgggcggca tctggaaatt
    10081 cgcgtcatgc cttataccca caaacagttg ttgatggtgg cgcgtgatgt cacgcaaatg
    10141 catcaactgg aaggggcgcg gcgtaacttt tttgccaacg tgagccatga gttacgtacg
    10201 ccattgaccg tgttacaggg ttacctggag atgatggatg agcagccgct ggaaggcgcg
    10261 gtacgcgaaa aagcgttgca caccatgcgc gagcagaccc agcggatgga agggctggtg
    10321 aagcagttgc taacgctgtc gaaaattgaa gcctcgccga cgcaattgct caatgaaaag
    10381 gttgatgtgc cgatgatgct gcgcgttgtt gagcgcgagg ctcagactct gagtcagaaa
    10441 aaacagacat ttacctttga gatagataac ggcctcaagg tgtctggcaa cgaagatcag
    10501 ctacgcagtg cgatttcgaa cctggtctat aacgccgtga atcatacgcc ggaaggcacg
    10561 catatcaccg tacgctggca gcgagtgccg cacggtgccg aatttagcgt tgaagataac
    10621 ggaccgggca ttgcaccgga gcatattccg cgcctgaccg agcgttttta tcgcgttgat
    10681 aaagcgcgtt cccggcaaac cggcggtagc ggattagggt tagcgatcgt gaaacatgcg
    10741 gtgaatcatc acgaaagtcg cctgaatatt gagagtacag taggaaaagg aacacgtttc
    10801 agttttgtta tcccggaacg tttaattgcc aaaaacagcg attaatccgc ctttgtcatc
    10861 ttttattgcc ataagccagc cgatgctggc ttattttctt tgcagtcaaa atacgggcgt
    10921 tagattttac aacggttggt gattttttgt tcgcatgatt agccatgtct ttttcacgga
    10981 aatagtgttt tatactggtt ggtgatttct tatcgctata tacctctggt ttttagatcc
    11041 ctccttgctt taaaacgtta taagcgttta aattgcgctt caggtgctgt catactgact
    11101 gcattaacgc ggtaaatcga aaaactattc ttcgccgcgc ctggttggga gtattttccg
    11161 ctaaaattgt ttaaatatac cgctgtatca tccccaggga ttggcacaaa aatttaacgt
    11221 tacaacacca catccacagg cagtatgatt tatgacccat caattaagat cgcgcgatat
    11281 catcgctctg ggctttatga catttgcgtt gttcgtcggc gcaggtaaca ttattttccc
    11341 tccaatggtc ggcttacagg caggcgaaca cgtctggact gcggcattcg gcttcctcat
    11401 tactgccgtt ggcctgccgg tattaacggt agtggcgctg gcaaaagttg gcggcggtgt
    11461 tgacagcctc agcacgccaa tcggtaaagt cgctggcgta ctgctggcaa cggtttgtta
    11521 cctggcggtg gggccgcttt tcgctacgcc gcgtacagct accgtttcct ttgaagtggg
    11581 gattgcgccg ctgacgggtg attccgcgct gccgctgttt atctacagcc tggtctattt
    11641 cgctatcgtt attctggttt cgctctatcc gggcaagctg ctggataccg tgggcaactt
    11701 ccttgcgccg ctgaaaatta tcgcgctggt catcctgtct gttgtcgcga ttgtctggcc
    11761 ggcgggttct atcagcacgg cgactgaggc ttatcaaaac gctgcatttt ctaacggctt
    11821 cgttaacggc tatctgacca tggatacgct gggcgcaatg gtgtttggta tcgttattgt
    11881 taacgcggcg cgttctcgtg gcgttaccga agcgcgtctg ctgacccgtt ataccgtctg
    11941 ggctggcctg atggcgggtg ttggtctgac tttgctgtac ctggcgctgt tctgtctggg
    12001 ttcagacagc gcgtcgctgg tcgatcagtc tgcaaacggc gctgctattc tgcatgctta
    12061 cgttcagcac acctttggcg gcggcggtag cttcctgctg gcggcgttaa tcttcatcgc
    12121 ctgtctggtc acggcggttg gcctgacctg tgcttgtgca gaattctttg cccagtacgt
    12181 accgctctct tatcgtacgc tggtgtttat cctcggcggc ttctcgatgg tggtgtctaa
    12241 cctcggctta agccagctga tccagatctc cgtaccggtg ctgaccgcta tttatccgcc
    12301 gtgtatcgca ctggttgtat taagttttac acgctcatgg tggcataatt cgtcccgcgt
    12361 gattgctccg ccgatgttta tcagcctgct ttttggtatt ctcgacggga tcaaagcatc
    12421 tgcattcagc gatatcttac cgtcctgggc gcagcgttta ccgctggccg aacaaggtct
    12481 ggcgtggtta atgccaacag tggtgatggt ggttctggcc attatctggg atcgcgcggc
    12541 aggtcgtcag gtgacctcca gcgctcacta aatcactgaa catttgtttt aaccacgggg
    12601 ctgcgatgcc ccgtggtttt ttattgtgtt gatgggttag gaattgatgg aaagtaagaa
    12661 taagctaaag cgtgggctaa gtacccgcca catacgcttt atggcactgg gttcagcaat
    12721 tggcaccggg ctgttttacg gttcggcaga cgccatcaaa atggccggtc cgagcgtgtt
    12781 gttggcctat attatcggtg gtatcgcggc gtatatcatt atgcgtgcgc tgggggaaat
    12841 gtcggtacat aacccggccg ccagctcttt ctcgcgttat gcgcaggaaa accttggccc
    12901 gctggcaggt tacattaccg gatggaccta ctgctttgaa atccttattg tcgccatcgc
    12961 cgatgtgacc gcttttggta tctatatggg ggtcttgttc ccgacggtgc cgcactggat
    13021 ttgggtactg agcgtggtgc tgatcatttg cgccgtaaac ctgatgagcg tgaaggtctt
    13081 cggtgagctg gaattctggt tctcgttctt taaagtcgcc accatcatca tcatgattgt
    13141 cgccggtttc ggcatcatca tctgggggat tggcaacggc gggcaaccga ccggtattca
    13201 taacctgtgg agcaacggcg gcttcttcag taacggctgg cttggcatgg taatgtcgtt
    13261 gcaaatggtg atgtttgctt acggtgggat cgaaattatc gggattaccg ccggtgaagc
    13321 gaaagatcct gagaaatcga ttccgcgtgc gattaactcc gtgccgatgc gtattctggt
    13381 gttctacgtc ggtacgctgt tcgtcattat gtctatctac ccgtggaatc aggttggcac
    13441 tgccggtagc ccgttcgtgc tgacgttcca gcatatgggc attacctttg ccgccagcat
    13501 tcttaacttt gttgtgctga ccgcttcgct gtcggcaatt aacagtgacg tatttggcgt
    13561 aggccgtatg ctccacggta tggcagagca gggcagcgca ccgaaaattt tcagcaaaac
    13621 gtcgcgtcgc ggtattccgt gggttacggt gctggtgatg actaccgcgc tgctgtttgc
    13681 ggtgtatctg aactacatca tgccggaaaa cgtcttcctg gtgatcgctt cgctggcaac
    13741 cttcgccacg gtatgggtgt ggattatgat cctgctgtcg caaatcgcct tccgtcgccg
    13801 tttgccgcca gaagaagtta aggcgctgaa atttaaagtg ccgggtgggg tagcaacgac
    13861 catcggcggg ctgattttcc tgctctttat tatcgggttg attggttatc acccggatac
    13921 gcgtatctcg ctgtatgtcg gtttcgcgtg gattgttgtg ctgttgattg gctggatgtt
    13981 taaacgccgc cacgatcgtc agctggctga aaaccaataa tacttgcgtt ctgtaagccg
    14041 gttcaggagg aaatcctgat ccggctattc cgaaagttat ccgcccccgt cctcctcccc
    14101 caaatatcct tcagatgatg agtgatcctg cattaggcta tggcaaggtg atcagatttt
    14161 catcacaggg gaattatgat gttaaatgca tggcacctgc cggtgccccc atttgttaaa
    14221 caaagcaaag atcaactgct cattacactg tggctgacgg gcgaagaccc accgcagcgc
    14281 attatgctgc gtacagaaca cgataacgaa gaaatgtcag taccaatgca taagcagcgc
    14341 agtcagccgc agccaggcgt caccgcatgg cgtgcggcga ttgatctctc cagcggacaa
    14401 ccccggcggc gttacagttt caaactgctg tggcacgatc gccagcgttg gtttacaccg
    14461 cagggcttca gccgaatgcc gccggcacga ctggagcagt ttgccgtcga tgtaccggat
    14521 atcggcccac aatgggctgc ggatcagatt ttttatcaga tcttccctga tcgttttgcg
    14581 cgtagtcttc ctcgtgaagc tgaacaggat catgtctatt accatcatgc agccggacaa
    14641 gagatcatct tgcgtgactg ggatgaaccg gtcacggcgc aggcgggcgg atcaacgttc
    14701 tatggcggcg atctggacgg gataagcgaa aaactgccgt atctgaaaaa gcttggcgtg
    14761 acagcgctgt atctcaatcc ggtgtttaaa gctcccagcg tacataaata cgataccgag
    14821 gattatcgcc atgtcgatcc gcagtttggc ggtgatgggg cgttgctgcg tttgcgacac
    14881 aatacgcagc agctgggaat gcggctggtg ctggacggcg tgtttaacca cagtggcgat
    14941 tccca
//
LOCUS       DAJVQH010000100        14743 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJVQH010000100 DAJVQH010000000
VERSION     DAJVQH010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.100"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            590..787
                     /locus_tag="OQM87_003044"
     CDS             590..787
                     /locus_tag="OQM87_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509103.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(737..1930)
                     /locus_tag="OQM87_003045"
     CDS             complement(737..1930)
                     /locus_tag="OQM87_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1509104.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2066..3790
                     /gene="iucA"
                     /locus_tag="OQM87_003046"
     CDS             2066..3790
                     /gene="iucA"
                     /locus_tag="OQM87_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCS1509105.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3791..4738
                     /gene="iucB"
                     /locus_tag="OQM87_003047"
     CDS             3791..4738
                     /gene="iucB"
                     /locus_tag="OQM87_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCS1509106.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4738..6480
                     /gene="iucC"
                     /locus_tag="OQM87_003048"
     CDS             4738..6480
                     /gene="iucC"
                     /locus_tag="OQM87_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCS1509107.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6477..7793
                     /gene="iucD"
                     /locus_tag="OQM87_003049"
     CDS             6477..7793
                     /gene="iucD"
                     /locus_tag="OQM87_003049"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCS1509108.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7799..9994
                     /gene="iutA"
                     /locus_tag="OQM87_003050"
     CDS             7799..9994
                     /gene="iutA"
                     /locus_tag="OQM87_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCS1509109.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10545..10688)
                     /locus_tag="OQM87_003051"
     CDS             complement(10545..10688)
                     /locus_tag="OQM87_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509110.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10938..>14743
                     /gene="sat"
                     /locus_tag="OQM87_003052"
     CDS             10938..>14743
                     /gene="sat"
                     /locus_tag="OQM87_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HCS1509111.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLR"
BASE COUNT         3977 a         3510 c         3665 g         3591 t
ORIGIN      
        1 aacaaaggca gcttatcgtt gtttttcaaa cgataaagtc tctgctaata aaatcatggc
       61 tcctcacaaa gaaaatatca ctgaaagagc tcagcagttt aaaagagttt tagttttaca
      121 ggatacaaca gaacttaatt attctggtca gaaagaaaaa caaggagttg gtcccaaaaa
      181 acataaagat gaaagaaact tatttcttca tcctcaactc gtcatcagtg agtcaggtgt
      241 ttgcctcggt gtttatgatg actatcagtg gttcagggat gaattaaaaa ccagtaaaaa
      301 tacccgacaa gaaatctgca atgatttact tcataaaaaa catgtcagtg aaaaagaaac
      361 ctggcgttgg gttgaaggat ataataaagc aacagaactg gctcgccaat gccctgatac
      421 ccatgtattg tcaatttctg atcgtgaagg cgatttttat gacctgtttg aacgcgcagc
      481 gcagactccc ggaataaaag ctgactggct tgtcagaatg aaatttaata acagagcaac
      541 gctgaatatt aatggtaaac gggatcatag gttgttgcac gaaagaatca tggagattac
      601 accgcagcaa ctggttgaat tcactatacc tgatgggcgg gggcagcaag ccagaacact
      661 gcctgacatg ctttcatcag acgcaggtcc tctctcatat cctctgattt actccagtaa
      721 ggtgatgatc atatctttat ttgatagtac ggtaatgcca gataaataca gacacaatgg
      781 taaataatat agttgcaaat aatgctgcca taatcagcgc gattgcgaca cctgttttcg
      841 ataccagcgg caccagcaaa gcgcacagtc catagccaag tgtgtgactg gtagcgagcc
      901 agcccgcgcc tgcgccatca gccagcttat cattcagtag aagttgataa gcgggtgttg
      961 ccagagctgc tccaaatgac agcactgcac agcccgggta aaacaaccat agctgttcgg
     1021 agagcatgat agccagacca ccactcatca acactccggc actcaggagc agggccaccg
     1081 gagtcaggcg ctgcggacgg agtaccccga actgcgcgat aagcgcagct actgcggaca
     1141 gacccaacag ccaggccacc tgttggctaa tggcggtggt atccgtagcg aactggcgag
     1201 taagggcagg cgaaagtcca agctgcatca tgctcaccgc cgcagccagt aatattgcgc
     1261 aaagcagata aggcagacaa tcccgcttca gactgacgct cttgcactcc ggtgtgggct
     1321 gtggtggtgt gccgggcaac cgcagcagca tcagcagcgc cagcactggg gctgccatca
     1381 gcagccccag tggcgccaga ggatgaatgg ccagcattgc tgccgcgcac agcggcccga
     1441 atagcctgcc gcaactcagg ccggagctga tggttgccag agcggccatg cgattccctt
     1501 ctccagctct ctgcaatgcc cagacctgac aggctggcac cattgccgac acggtcagcc
     1561 cgtaggcgat ccgggcgatg attaatattc ccagtcccac cgctgttgtt atggcgctgg
     1621 tagccatcag cacgcttccc agccctagca gggtaaagct tatgccatat cctcccagag
     1681 cccacaacac cacaggttta ctgccagtac gggaaatttg ctttccccac cacggggacg
     1741 atggcagaaa cagcattgag ccaagcatca gcagggcagc ccatacagac agactcagat
     1801 ttgtctgtat gaccagaaca gggagcacaa ccagcagacc gttctgtccg ataccgagaa
     1861 gcccggcact gaacgcaagt ggccagcagg acagtggttt ttggggcgta tcttcgatcc
     1921 ctggtgacat tataatgttt caactccatg tattaattgt gtttatttgt aaaattaatt
     1981 tatctgacaa taacatttat tgttgataat gagaatcatt attgacataa ttgttattat
     2041 tttactgtgt gggagctgtt tgactatgac cctgccctct gaaaaaccag ccacagatgt
     2101 ggctgcgcag tgcttcctga atgcactgat tcgtgaaacc acagactgga aactgacaga
     2161 atacccgcca gacgaattgc tcatcccgct ggatgagcag aaatcgctcc atttcagagt
     2221 ggcttatttc tccccaaccc agcatcaccg ctttgcattt cctgcacgtc tggtcacggc
     2281 atcaggcagt tatcctgtcg actttaccac tctctcccgg cttattattg ataagctacg
     2341 ccatcaactg tttctgcccg ttcccctctg cgaaactttc caccagcgcg tgctggaaag
     2401 ccatgtccat acgcaacagg caattgatgc ccgtcatgac tgggccgctc tgcgtgaaaa
     2461 agcgttgaat tttggcgagg ctgagcaggc gctgctgaca ggacacgctt tccaccctgc
     2521 gcctaagtct catgaaccgt ttaaccggcg ggaggctgaa cgctacctgc ctgatatggc
     2581 accccacttc ccactgcggt ggttttcggt ggataaaacg caaatcgccg gtgaaagttt
     2641 gcatctcaac cttcaacagc ggctgacgcg atttgccgca gagaatgcgc ctcagttact
     2701 caacgaatta agtgacaacc aatggctgtt cccgttgcac ccgtggcagg gggaatatct
     2761 tttgcagcag gggtggtgcc aggcacttgt tgctaaaggg ctgattaaag acttaggtga
     2821 ggccggcacg tcgtggctgc cgaccacctc ttcccgttcc ctctactgtg ccaccagccg
     2881 cgatatgatc aagttctccc tgagcgtacg gctgaccaac tccatccgta ctctgtccgt
     2941 gaaagaagtg aagcgtggga tgcgcctggc acgcctggct caaaccgacg gctggcagat
     3001 gctacaggtc cgcttcccga ctttccgggt aatgcaggag gatggctggg ccgggctgct
     3061 cgatcttaac ggcaacatca tgcaggaaag tctgtttgcc ctgcgtgaaa atctgctggt
     3121 ggatcagccg aaaagccaga ctaacgtact ggtctccctg actcaggccg cacctgatgg
     3181 cggtgattcg ctgctggttt cggcggtaaa acgcctgagc gatcgcctcg gtatcactgt
     3241 gcaacaggcc gcccatgcat gggtcgatgc atactgtcag caagtgctaa agccgctgtt
     3301 tacggctgaa gcggattacg gcctggtgct gctggcgcat cagcaaaata ttcttgtcca
     3361 gatgcttggg gatctgccgg tcggatttat ttaccgtgac tgtcagggca gcgcttttat
     3421 gcctcacgcg acagactggc tcgattctat tggcgaggcg caggcggaaa atattttcac
     3481 ccatgagcag ttgctgcgct atttccctta ttacctgctg gttaactcca cttttgctgt
     3541 gaccgctgcg ctgggggctg ccgggctgga cagcgaatcg aatctgatgg ctcgtgtacg
     3601 agcatcgctg gctgaagtgc gtgatcaggt gactcataaa acctgtctca actacgtgct
     3661 ggaaagtccg tactggaacg taaaaggtaa ctttttctgt tatctgaacg atcataacga
     3721 gaacaccatt gttgaccctt cggtgatcta tttcgatttc gctaacccgc tgcaggctca
     3781 ggaggtctga atgtctgagg caaacattat tcacagcaga tatggactgc gctgtgaaaa
     3841 actcgacaag cccctgaatc ttggctgggg actggacaat agcgcggtgt tgcactgtcc
     3901 cggggagctg ccgacagggt ggctgtgcga tgcgctggat cagatattta tcgccgcacc
     3961 acaactgtca gcagtggctc tcccctgggc cgaatggcgt gaggagccac aggcgctgac
     4021 gctttttggg caggtaaaaa gcgacattat ccatcgtact gccttctggc agttaccgtt
     4081 atggttgagt tcaccggcaa accgggcctc cggtgaaatg gtttttgatg cagagcgtga
     4141 gatttatttc ccgcagcgcc caccccgtcc acagggtgag gtttaccgcc gttacgatcc
     4201 acgtattcgc aggatgctga gtttccgcat tgccgatccc gtttctgatg cagaacgttt
     4261 cacccgctgg atgaatgatc cgcgcgttga gtatttctgg gagcaaagtg gctcactgga
     4321 ggtacagacc gcctatctgg agcgccagct caccggtaaa catgcattcc cgctgattgg
     4381 gtgcttcgac gatcggccat tcagctattt cgaaatctac tgggcggcgg aagaccgcat
     4441 tggtcgtcac tattcgtggc agccctttga ccgtggcctg catctgctgg ttggtgaaca
     4501 gcaatggcgc ggggcccact atgtgcaaag ctggctgcgc gggctgacac attacctgct
     4561 gctggatgag ccccgtacgc agcgcacagt actggagcca cgcaccgata accagcgcct
     4621 gttccgccat cttgagcctg cgggataccg gacaattaaa gagttcgact tcccacataa
     4681 gcgctcgcgc atggtgatgg cggatcgtca tcacttcttc acggaggtcg gtctgtaatg
     4741 aatcacaagg actgggattt tgttaaccgt cggctggtgg caaaaatgtt gtctgagatg
     4801 gagtatgagc aggttttcca cgcggaatct cagggcgatg accactactg cattaacctg
     4861 ccgggagcac aatggcgctt catcgctgaa cgtggtatct ggggctggct gtggattgat
     4921 gcccaaacgt tgcgctgcac ggacgaacca gtactggcgc agacgctgct gatgcagcta
     4981 aagccggtgc tgtcaatgag tgatgccact gttgcagagc atatgcagga tttgtatgcc
     5041 acgctactgg gcgacctgca actgctgaag gcccgtcgcg gactgagtgc cagtgacctg
     5101 attaatcttg atgccgatcg tctgcaatgc ctgctgagcg gtcatcctaa attcgttttt
     5161 aataaaggtc gccgtggctg gggcaaagag gcgctggaac gatatgcgcc agagtatacc
     5221 aacaccttca gactgcactg gctggcggta aaacgtgaac atatgatctg gcgctgcgat
     5281 aacgatctgg atattcagca gttgttgact gccgcaatgg atccgcagga gtttacacgc
     5341 ttcagtcagg tctggcagga aaacgggctg gatcacaact ggctgccgct gccggtacat
     5401 ccgtggcagt ggcagcaaaa aatcgccaca gacttcatcg ctgattttgc cgaaggcagg
     5461 atggtgtctc tcggcgagtt tggcgaccag tggctcgccc agcagtcgct gcgtaccctg
     5521 accaacgcca gccgccgggg agggctggat atcaagctgc cgctgaccat ctacaatacc
     5581 tcatgctacc gggggatccc tggcagatat atcgctgccg gaccactggc ttcacgctgg
     5641 ctgcaacagg tttttgcgac cgacgccacc ctggtgcaaa gcggcgcagt gatccttggt
     5701 gaaccggctg caggctatgt gtcccatgaa ggctatgccg cgcttgcccg ggctccctat
     5761 cgctaccagg aaatgcttgg tgttatctgg cgggagaatc cgtgccgctg gctgaaaccg
     5821 gatgaaagtc ctgttctgat ggcaacactg atggagtgcg acgaaaacaa tcagccgctg
     5881 gcaggcgcat atatcgaccg ctccggactg gacgctgaaa cctggcttac gcaattgttc
     5941 cgggtggttg tggttccgct gtatcacctg ctttgccgct acggtgtcgc gcttattgca
     6001 cacggacaaa atataacgct cgccatgaaa gagggggtac cacagcgtgt tctgctgaaa
     6061 gacttccagg gcgatatgcg gctggtgaaa gaagcgtttc ccgaaatgga ctctttgcct
     6121 caggaggtgc gtgatgtcac atcccgtctg agtgcggact acttaatcca tgatttgcag
     6181 acgggtcact tcgtgacagt actgcgtttt atttcgccac tgatggttcg tcttggcgta
     6241 cctgaaaggc gattttatca actgctggca gaagtgttga gtgattacat gaacaaacat
     6301 ccacaaatgg cagagcgttt tgcgcttttc tcactcttca ggccacaaat cattcgcgtg
     6361 gtgctgaacc cggtaaaact gacctggccg gacctggatg gcggcagtcg catgctgccg
     6421 aattaccttg agaatctgca aaatccgctg tggctggtaa ctcaggaata tgaatcatga
     6481 aaaaaagtgt cgattttatt ggtgtcggga cagggccatt taatctcagc attgctgcat
     6541 tgtcacatca gatcgaagaa ctgaactgtc tcttctttga cgaacatcct catttttcct
     6601 ggcatccggg tatgctggta ccggattgtc atatgcagac cgtctttctg aaagatctgg
     6661 tcagtgcagt tgcacctaca aatccctaca gctttgttaa ctatctggtg aagcacaaaa
     6721 agttctatcg cttccttaca agcaggctac gtacagtatc ccgtgaagag ttttctgact
     6781 atctccgctg ggctgctgaa gatatgaata acctgtattt cagtcatacc gttgaaaata
     6841 ttgatttcga caaaaaaagt cgattgtttc tggttcagac cagtcgggga gaatattttg
     6901 cccacaatat ctgccttggt acaggaaaac aaccttattt accaccctgt gtgaagcatg
     6961 tgacacaatc ctgtttccat gccagtgaaa tgaatcttcg tcggccggac ctgagtggaa
     7021 aacggataac cgtggttggt ggaggacaga gtggtgcaga cctgttcctt aatgcattac
     7081 gcggggaatg gggagaagcg gcggaaataa actgggtctc acggcgtaat aattttaacg
     7141 cactggatga ggctgctttt gctgatgagt attttacacc tgaatatatt tcaggcttct
     7201 ccggactgaa ggaagatatt cgccatcagt tactggatga gcagaaaatg acatcggatg
     7261 gcatcactgc cgattcttta ctgaccattt atcgtgagtt gtaccaccgt tttgaagttc
     7321 tgagaaaacc aagaaatatc cgtctgctac ccagtcgctc ggtaacaact ctggaaagta
     7381 gtggtccggg ctggaggtta ttgatggaac atcatctgga tcagggcaga gagagcctgg
     7441 aaagtgatgt ggtgattttc gccacaggtt accgttctgc attgccacaa atacttccct
     7501 cactgatgcc cttgatcacc atgcacgata agaacacctt taaagtgcgt gatgacttca
     7561 ctctggaatg gagtggcccg aaagaaaata acatctttgc ggtcaacgcc agcatgcaaa
     7621 ctcatggcat cgccgaaccc cagctcagcc tgatggcctg gcgatctgca cgtattctta
     7681 atcgcgtact ggggcgtgat ttattcgatc tcagtatgcc gcccgcactg attcagtggc
     7741 gcagcggcag ccctgcttct ttaactcact atacagcaaa tattcaggaa taacgatgat
     7801 gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt cttgtcgtgc tgtcaccagc
     7861 tgtcgcccag caaaacgatg ataatgagat catagtgtct gccagccgca gcaatcgaac
     7921 tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat gccgaactgg agcagcagat
     7981 tcagggcggt aaagagctga aagacgcact ggctcagtta atccccggcc ttgatgtcag
     8041 cagccaaagt cgaaccaact acggtatgaa catgcgtggc cgcccgctgg ttgtcctgat
     8101 tgacggtgtg cgcctcaact cttcacgttc cgacagccga caactggact ctgtcgatcc
     8161 ttttaatatc gaccatattg aagtgatctc cggcgcgacg gccctgtacg gtggcgggag
     8221 taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag ccggaaacca tgatggagtt
     8281 tgaggctggc acaaaaagtg gctttaacag cagtaaagat cacgatgagc gcattgccgg
     8341 tgctgtctcc ggcggaaatg accatatctc cggacgtctt tccgtggcat atcagaaatt
     8401 tggcggctgg tttgacggta acggcgatgc caccctgctt gataacaccc agaccggcct
     8461 gcagcactcc aatcggctgg acatcatggg aaccggtacg ctgaacatcg atgaatcccg
     8521 gcagcttcaa ctgataacgc agtactataa aagtcaggga gacgacaatt acgggcttaa
     8581 tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca ccatacgtca gtaaggggct
     8641 gaattctgac cgcattcccg gcactgagcg gcatttgatc agcctgcagt actctgacag
     8701 tgatttcctg ggacaggaac tggtcggtca ggtttactac cgcgatgagt cgttgcggta
     8761 ctacccgttc ccgacggtaa atgcgaataa acaggcgacg gctttctcct cgtcacagca
     8821 ggataccgac cagtacggca tgaaactgac tctgaacagc caactgatgg acggctggca
     8881 aatcacctgg gggctggatg ctgagcatga gcgctttacc tccaaccaga tgttcttcga
     8941 tctggctcag gcaagtgctt ccggagggct gaacaaccat aagatttaca ccaccgggcg
     9001 ctatccgtca tatgacatca ccaatctggc ggccttcctg caatccagct atgacattaa
     9061 tgatattttt accgttagcg gtggcgtacg ctatcagtat actgagaaca gggtagatga
     9121 tttcatcgac tacacgcagc aacagaagat tgctgccggg aaggcgatat ctgccgacgc
     9181 cattcctggt ggttcggtag attacgataa ctttctgttc aatgctggtc tgctgatgca
     9241 catcaccgaa cgtcagcagg catggttcaa tttttcccag ggggtggcat tgccggatcc
     9301 ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg aacggccatc ttcccctgac
     9361 aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg aaagtcgatt cttatgaact
     9421 gggctggcgc tttatcggtg acaacctgcg gactcaaatc gcggcatatt actcgctttc
     9481 caataagagc gtggaaagga ataaagatct gaccatcagt gtgaaggacg acaggcgccg
     9541 tatttacggc gtggaaggtg cggtggacta cctgatcccg gatactgact ggagtaccgg
     9601 tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac ggtcaatggc aaaaatatga
     9661 cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt aactgggcgc cggaaccgtg
     9721 gagtctgcgt gtacagagca ccacttcttt cgacgtaagc gatgcagagg gtaacgatat
     9781 taatggttac actaccgtcg attttatcag tagttggcag cttccggtgg gaacactcag
     9841 cttcagcgtt gagaacctct tcgaccgtga ctataccact gtctggggac agcgtgcacc
     9901 tctgtactac agcccgggtt acggccctgc ttcactgtac gactacaaag gccggggccg
     9961 aacctttggt ctgaactact cagtgctgtt ctgacgggga tgcttttgca acaagggtat
    10021 gctgtaaaca acatggtcct tggtggccat gttgtttaca gccagtataa tatacagcct
    10081 taacaagata ttccgtctcc ggaatgggct gttcaccact gaataacatt atgtctgttt
    10141 tatggatctt tgactgaaat ttatcgcata tcctcatttc attgtccgtc tgaaaactaa
    10201 ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc ctgaaattcc tgagaggatg
    10261 atctgcgccc aatatcctga gccggaactt tgctgcaatg aaggccaacg aaaaatgggg
    10321 gagtatgtta tagagtactt gctacagggt aaaaagaggt acctgtcgct ggttctccgt
    10381 tcagtttaac cgggagattt tctcctgcgc ctgtcataaa gtccggtaat ggagattgtt
    10441 aatacgatgg tgcacgatgc atcgtcagtg cccggcccgg aggatgcccc gtagctacat
    10501 acggatcagg gttggcaata tcgaatggca ggctataggc aaagttaaag ccccatggag
    10561 tagcacaaaa tatgccgcac aaaggaaacg gtctgaataa cgcagtgatg gagaacttct
    10621 tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt cgtcgtgaat cactgaacga
    10681 tgttccatgc gaacagatat tcgatcatat cgaaatatta aacattaccc ctccccacat
    10741 tgggcaaaaa aaaacaaaaa acgatcaatt aacgatcaat tagtattgat tcacataatc
    10801 tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta cttttagcaa
    10861 gctgttagta accaaccgtt actaataatt ttaattctta atactttgtt cattcaataa
    10921 atgagttgag agaatatatg aataaaatat actcccttaa atatagtgct gccactggcg
    10981 gactcattgc tgtttctgaa ttagcgaaaa gagtttctgg taaaacaaac cgaaaacttg
    11041 tagcaacaat gttgtctctg gctgttgcag gtacagtaaa tgcagcaaat attgatatat
    11101 caaatgtatg ggcgagagac tatcttgatc ttgcacaaaa taaaggtatt ttccagcccg
    11161 gagcaacaga cgtaacaatc actttaaaaa acggagataa attctctttc cataatctct
    11221 caattccgga tttttctggt gcagcagcga gtggcgcagc taccgcaata ggaggttctt
    11281 atagtgttac tgttgcacat aacaaaaaga accctcaggc cgcagaaact caggtttacg
    11341 ctcagtcttc ttacaaggtt gttgacagaa gaaattccaa tgattttgag attcagaggt
    11401 taaataaatt tgttgtggaa acagtaggtg ccaccccgga agagaccaac cctacaacat
    11461 attctgatgc attagaacgc tacggtatag tcacttctga cggttcaaaa aaaatcatag
    11521 gttttcgtgc tggctctgga ggaacatcat ttattaatgg tgaatccaaa atctcaacaa
    11581 attcagcata tagccatgac ctgttaagtg ctagtctatt tgaggtcacc caatgggact
    11641 catacggcat gatgatttat aaaaatgata aaacatttcg taatcttgaa atattcggag
    11701 acagcggctc tggagcatac ttatatgata acaaactaga aaaatgggta ttagtcggaa
    11761 caacccatgg tattgccagc gttaatggtg accaactgac atggataaca aaatacaatg
    11821 ataaactggt tagtgagtta aaagatacct atagtcataa aataaatctg aatggcaata
    11881 atgtaaccat taaaaacaca gatataacat tacaccaaaa caatgcagat accactggta
    11941 ctcaagaaaa aataactaaa gacaaagata ttgtgttcac aaatggggga aatgttctgt
    12001 ttaaggataa tttggatttt ggtagcggtg gtattatctt tgacgaaggc catgaatata
    12061 acataaacgg tcagggattt acatttaaag gagcaggaat tgatatcgga aaagaaagca
    12121 ttgtaaactg gaatgcattg tattccagtg atgatgtttt acacaaaata ggccccggta
    12181 ctctgaatgt tcaaaaaaaa cagggggcaa atataaagat aggtgaagga aatgttattc
    12241 ttaatgaaga aggaacattt aacaatatat accttgcaag cggaaatggt aaggtaatac
    12301 taaataaaga taattccctt ggcaatgatc aatatgcggg gatatttttt actaaacgtg
    12361 gtggcacgct agatttaaat ggacacaatc agacttttac tagaattgcc gccactgacg
    12421 atggaacaac aataactaac tcagatacaa cgaaagaagc cgttctggca atcaataacg
    12481 aagactccta catatatcat gggaacataa atggcaatat aaaactaacg cacaatatta
    12541 attctcagga taagaaaact aatgcaaaat taattctgga tggtagtgtc aacacaaaaa
    12601 atgatgttga agtcagtaat gccagtctta ccatgcaagg ccatgcaaca gagcatgcaa
    12661 tattcagaag cacaacgagt cattgctccc tggtatttct ttgtggaacg gactgggtca
    12721 ccgttttgaa agaaacagag agttcatata ataaaaagtt caattctgat tacaaaagta
    12781 ataatcagca gacctcattt gatcagcctg actggaaaac cggggtgttt aaatttgata
    12841 cattacacct gaacaatgct gacttttcaa tatcacgcaa tgccaatgtt gaaggaaata
    12901 tatcagcaaa taaatcagct atcacaatcg gcgataaaaa tgcttacatt gataatcttg
    12961 cagggaaaaa tattactaat aatggttttg acttcaaaca aactatcagt actaatctat
    13021 ccataggaga aactaaattt acaggtggca tcactgcaca taacagccaa atagccatag
    13081 gtgatcaagc tgtagttaca cttaatggtg caacctttct gaataatact cctataagta
    13141 tagataaagg agcaaaagtt atagcacaaa attccatgtt cacaacaaaa ggtattgata
    13201 tctccggtga actgactatg atgggaatcc ctgaacagaa tagtaaaact gtaacgccgg
    13261 gtctccacta cgctgctgat ggattcaggc tgagtggtgg aaatgcaaat ttcattgcca
    13321 gaaatatggc atctgtcacc ggaaatattt atgctgatga tgcagcaacc attactctgg
    13381 gacagcctga aactgaaaca ccgactatat cgtctgctta tcaggcatgg gcagagactc
    13441 ttttgtatgg ctttgatacc gcctatcgag gcgcaataac agcccccaaa gctacagtta
    13501 gcatgaataa tgcgatctgg catctaaata gccagtcatc aattaatcgt ctagaaacaa
    13561 aagacagtat ggtgcgtttt actggtgaca atgggaagtt tacaaccctt acagtggaca
    13621 accttactat agatgacagt gcatttgtgc tgcgtgcaaa tctggcccaa gcagatcagc
    13681 ttgttgtcaa taaatcgttg tctggtaaaa acaaccttct gttagtcgac ttcattgaga
    13741 aaaatggaaa cagcaacgga ctgaatatcg atctggtcag cgcaccaaaa ggaactgcag
    13801 tagatgtctt taaagctacg actcggagta ttggcttcag tgatgtaaca ccggttatcg
    13861 agcaaaagaa cgatacagac aaagcaacat ggactctgat cggctataaa tctgtggcca
    13921 acgccgatgc ggctaaaaag gcaacattac tgatgtcagg cggctataaa gccttccttg
    13981 ctgaggtcaa caaccttaac aaacgtatgg gtgatctgcg tgacattaac ggtgagtccg
    14041 gtgcatgggc ccgaatcatg agcggaaccg ggtctgccgg cggtggattc agtgacaact
    14101 acacccacgt tcaggtcggt gcggataaca aacatgaact cgatggcctt gacctcttca
    14161 ccggggtgac catgacctat accgacagcc atgcaggcag tgatgccttc agtggtgaaa
    14221 cgaagtctgt gggtgccggt ctctatgcct ctgccatgtt tgagtccgga gcatatatcg
    14281 acctcatcgg taagtacgtt caccatgaca acgagtatac cgcaactttc gccggccttg
    14341 gcaccagaga ctacagctcc cactcctggt atgccggtgc ggaagtcggt taccgttacc
    14401 atgtaactga ctctgcatgg attgagccgc aggcggaact tgtttacggt gctgtatccg
    14461 ggaaacagtt ctcctggaag gaccagggaa tgaacctcac catgaaggat aaggacttta
    14521 atccgctgat tgggcgtacc ggtgttgatg tgggtaaatc cttctccggt aaggactgga
    14581 aagtcacagc ccgcgccggc cttggctacc agtttgacct gtttgccaac ggtgaaaccg
    14641 tactgcgtga tgcgtccggt gagaaacgta tcaaaggtga aaaagacggt cgtatgctca
    14701 tgaatgttgg tctcaatgcc gaaattcgcg ataatcttcg ctt
//
LOCUS       DAJVQH010000101        14677 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJVQH010000101 DAJVQH010000000
VERSION     DAJVQH010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.101"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            146..1186
                     /locus_tag="OQM87_003053"
     CDS             146..1186
                     /locus_tag="OQM87_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCS1509112.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1219..2412)
                     /gene="lplT"
                     /locus_tag="OQM87_003054"
     CDS             complement(1219..2412)
                     /gene="lplT"
                     /locus_tag="OQM87_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCS1509113.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2405..4564)
                     /gene="aas"
                     /locus_tag="OQM87_003055"
     CDS             complement(2405..4564)
                     /gene="aas"
                     /locus_tag="OQM87_003055"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCS1509114.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            4953..5984
                     /gene="galR"
                     /locus_tag="OQM87_003056"
     CDS             4953..5984
                     /gene="galR"
                     /locus_tag="OQM87_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCS1509115.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(5991..7253)
                     /gene="lysA"
                     /locus_tag="OQM87_003057"
     CDS             complement(5991..7253)
                     /gene="lysA"
                     /locus_tag="OQM87_003057"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCS1509116.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7375..8310
                     /gene="lysR"
                     /locus_tag="OQM87_003058"
     CDS             7375..8310
                     /gene="lysR"
                     /locus_tag="OQM87_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCS1509117.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8297..8989)
                     /gene="ygeA"
                     /locus_tag="OQM87_003059"
     CDS             complement(8297..8989)
                     /gene="ygeA"
                     /locus_tag="OQM87_003059"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCS1509118.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAS
                     IHAEDAVAFMLS"
     gene            complement(9118..10506)
                     /gene="araE"
                     /locus_tag="OQM87_003060"
                     /pseudo
     CDS             complement(9118..10506)
                     /gene="araE"
                     /locus_tag="OQM87_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(10821..11582)
                     /gene="kduD"
                     /locus_tag="OQM87_003061"
     CDS             complement(10821..11582)
                     /gene="kduD"
                     /locus_tag="OQM87_003061"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCS1509119.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11612..12448)
                     /gene="kduI"
                     /locus_tag="OQM87_003062"
     CDS             complement(11612..12448)
                     /gene="kduI"
                     /locus_tag="OQM87_003062"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCS1509120.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(12768..13916)
                     /locus_tag="OQM87_003063"
     CDS             complement(12768..13916)
                     /locus_tag="OQM87_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCS1509121.1"
                     /translation="MKDVVVVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            14171..14677
                     /locus_tag="OQM87_003064"
                     /pseudo
     CDS             14171..14677
                     /locus_tag="OQM87_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
BASE COUNT         3799 a         3977 c         3469 g         3432 t
ORIGIN      
        1 ttaaccgtga tgatgtttcg caaattattg aacgttaaca aataaggtca gcatccggct
       61 ggccttaaga tttttctctt tcccttttcc cttccctctg ccatttttat attccttatg
      121 tcgtgattat aaaaaggaaa cggctatgca atatcaccgt ataccccaca gttcgctgga
      181 agtcagcacg ctggggcttg gcacgatgac gtttggtgaa cagaacagcg aagccgacgc
      241 ccacgcacaa ctcgactatg ccgtcgctca gggcattaac cttatcgacg ttgccgaaat
      301 gtacccagta cctccgcgcc ccgaaacgca agggttaacc gaaacctacg tcggcaactg
      361 gctggcgaaa catggcagcc gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc
      421 gcgcaataat gacaagggca tccgcccgga tcaggcgctg gatcggaaga atatccgcga
      481 agcgctgcat gacagcctca agcgcctaca gactgattac ctcgatcttt atcaggtgca
      541 ctggccgcag cgcccgacca actgcttcgg caaactcggt tatagctgga cggattctgc
      601 gcctgcggtt tcgctgctgg atacgctgga cgcactggca gagtaccaac gcgcgggaaa
      661 aattcgttat atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct acctgcatct
      721 ggcggacaaa cacgatctgc cgcgtattgt caccattcag aacccttaca gtctgttaaa
      781 ccgcagtttt gaagtaggtc tggcagaagt cagccagtat gaaggggtcg aactgctggc
      841 ctattcgtgc ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg caaaacccgc
      901 tggcgcacgt aatacgctct ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa
      961 agccgtcgcg gcgtatgttg atatcgccag acgtcatggc cttgatcctg ctcagatggc
     1021 gctcgcgttt gttcgccgtc aaccgtttgt tgccagcact ctgctgggcg caaccacgat
     1081 ggatcagctg aaaactaaca tcgaaagttt gcatctggag ttaagcgaag acgtattagc
     1141 tgaaattgaa gcggtacatc aggtttatac ttatcctgca ccataaagat taacgccggt
     1201 taaaaccggc gttaaatatt aatgacggcg ctgccagatc cacagcgccg ttattgccag
     1261 cgcaaatagc gcaccgaagc caatgccaat gggcacgacc gggatgccta ccattaccgc
     1321 cagcgagtaa atgcccagca tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc
     1381 aatcgcattc cccgccccga cgcttttttt accccgctcc tgtagcaacg cattgagcgg
     1441 aacgacaaaa aagcccccca gcacgccaat cagcatcaac aaggcatagg ctggcagcag
     1501 ctcgtgttgc agggagaaaa tcagtaccac cacgccaatc aaaatcccgg ctggcataca
     1561 gcgtgacacg gtttccagcg taaccagctt cgctgccgca cctgcgccaa ccacgatacc
     1621 aatcgctacc atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac
     1681 cggtacccac agcaccaaca ggaaacgcag cgtgacaccc gctccccaga ataaactggt
     1741 gcccaccagc gaaaaacgcg tttcaccatt gcaccatagc gaggtgcagg cattcaggaa
     1801 actgcgggtc atgttgatga gattccagga ctgccccgga cgcgccgccg ccagtttggg
     1861 aatgtagata ttggcaacga ccgcaccacc gtaggccagt gcgcatgcgg ccagggcgac
     1921 caggacatgc cagtcagcca gcacaccacc ggctacggaa ccgagtaaaa tcgccgctat
     1981 ggcagaagct tccattaaac cgttagcttt tactaactta ctacccgtgg ttaattcgcc
     2041 gagaataccg tatttcgccg gtgaataggc tgcagcacca acacccacca gcgtatagcc
     2101 gagaaacgga ttgataccaa agcagatact ggctgcgccc agcagcttca ggccgttggc
     2161 aaacatcatc acccggcctt tggcgaagct atccgccacc tgcccgacaa acggcgccaa
     2221 aagaatgtaa gcacctacaa acaccatttg caggatgggc tggctccact ccggatagaa
     2281 ctgcgctttc agtaacgcca gagtggcaaa cagtagcgcg ttatcgccaa acgcagagag
     2341 aaactgcgcc acgataaccg ctttcatccc cttcgaccac aacgaagtgt tagtgtgcac
     2401 tgactcactc atcgtgttgt tccgcttcgt ctacccagct tttcaacgtg acaaagtcag
     2461 gtttgccgct gccaagtaat ggcatctgtt tcagatagcg aatatcgcgc ggtacagcaa
     2521 gctccggcac gccgtgctcg cgggcatact gttgcaactt atcgcgcgtc agttcgttat
     2581 ctgtggtgaa aagcaccagt gcctcgcctt tgctggcatc gctcttaatc gcagtggcat
     2641 ggactttatc tggcgaaaca ccaagtgcca gttgttccac catttccagc gacaccattt
     2701 cgcctgcaat tttggcaaag cgttttgcgc ggccctgaat ctgcacaaag ccctgctcgt
     2761 caaaacgcac aatatcgcca gtgtcatacc agccgcgctc catttcgccg cgaacattct
     2821 cggcggtggg cacttccagt acacctggct tctccacccg cagatagccg ttcattatgt
     2881 tcggcccttt cagttgcagg cgtccgccct cttcgatacc agggaccgac aacaggcgtg
     2941 catccattcc tggtagaata cgccctaccg taccgggttt cgccgccatc ggtacgttga
     3001 tagaaacgac aggcgcgcat tcggtcacac cgtagccttc aaggatgcgc aggccaaatt
     3061 tatcctgcca aagctgtttg gtactttctt gtagtttttc tgcgcctgcc accacatagc
     3121 gtagacgata gaagtcatac gggttggcga aacgcgcgta gtgaccgagg aaagtcgagg
     3181 taccgaacaa cacggtgcaa ctgcggtcat acaccaagtc cggcacaatg cggtaatgca
     3241 gcgggcttgg ataaaggaac acttctgcac ctgtaagcag tggcgtaaac aggcctaccg
     3301 tcagcccaaa ggagtgaaac agcggtaatg ccgacataaa gcgatcgttg gtggtgaagt
     3361 cggcaatcgt tttaatctgc tcgacgttcg ccagaatgct tttatggcta tggacgacgc
     3421 cttttggatg gccttcagaa ccggaggtaa agaggatcag cgcctcttct tccggctgct
     3481 gttttacctg cgccaggcgc ggcatcagca aatgagcgaa gatccatact ttgtcggcag
     3541 tggtgacatc tgcttttaaa tcttccagat agacccagcg cacctgagta agttgctccg
     3601 gcagatgcca gagtttgcct ttatcgagaa actggcggga agtgaagatg gttttgattt
     3661 cagccgccgt aatagcactg gtcagccctt ttaccccggc agtgtagttc atcattgcgg
     3721 gaatgcggcg acgcgcgatg gccccaaaaa tcactgccgc actgatgcct gcattgggca
     3781 gcattaagcc gatgcgttcg ccttcaacac tgtatttttc aaggatgcgt ccaacaaaca
     3841 gcgttttcgt aagcaatttg cgataggagt ctggggtaaa gttgacgtct tcgacacatt
     3901 tcttcccggc tccgaagcgg tacattgcac tcagtaaaga ttcgtacaga gtttcacgcg
     3961 ggcgcaccgc catacgcgct tccatcatta tttgatgcag catttcgcca gcgattttgc
     4021 gacggtcacg ggcacgcggc gcatccggca tcgccacctg cgttggaggc aaaatatgca
     4081 gagtaatttg cgggaacaag cgacgtttaa ccagaccttt caggcggctg aagtgcgtaa
     4141 gttccgcccc ttcaatacgc acaggaataa ccgttgcacc agacttcgcc gcgacaaaac
     4201 ccgcgccatc gtagattttc atcagcgagc ctgtagtggt gatgcgtcct tcagggaaaa
     4261 tcaccactgg tcggccctgt tccaccagac gtaccagatg tttaatagcc ataggttgcg
     4321 tcgggtcgag aggaacaaag tcgataaatg atttcagcca acgcatatac cactgttggc
     4381 ttattgaggt gtaaacggca aacactggac gcacaggtaa aaacagtcca agcaaaatgc
     4441 catcaataaa agagacgtga ttaggcgtaa ttagaacgcg ctcgcccttc agtgcctggg
     4501 tgtcacccgt aacgcgaacg cgatacaaaa cacggcacaa atttcgaaaa aagctaaaaa
     4561 gcattccaac tccctttgct ctgattcagt aaaagcgaat ggagggagat tacacgagat
     4621 aaagaacgcg agcgacagta aattaggtgc gaaaaaaaac ctgcgcatcc gcgcaggttg
     4681 gtgcaagaga cagggtacga agagcgtacc gaataatctc accaatcaat acctctggga
     4741 tcaccacttt agcaacctga agccaaacgc caccagcggt caatcgcaac agcgtttcgc
     4801 aaagtgtaac caaaggtttg caatctcttt ttttgtctta cttatttgcg tttggctcac
     4861 attcccacga tgaaaacacg ccaccccttg aaccaacggg cgttttccgt aacactgaaa
     4921 caatgtaagc gtttacccac taaggtattt tcatggcgac cataaaggat gtagcccgac
     4981 tggcaggcgt ttcagtcgcc accgtttccc gcgtcattaa taattcaccc aaagccagcg
     5041 aagcttcccg gctggctgtg catagtgcaa tggagtctct tagctatcac ccgaacgcca
     5101 acgcccgtgc gctggcgcag cagaccactg aaacggtcgg tctgatcgtt ggtgatgttt
     5161 ccgatccgtt tttcggtgca atggtgaaag cggtcgaaca ggtggcttat cacaccggta
     5221 atttcttatt gattggcaac ggttaccaca acgaacaaaa agagcgtcag gctattgagc
     5281 aactgatccg ccatcgctgt gctgcgttgg tcgtccatgc caaaatgatc ccggatgccg
     5341 atttagcctc attaatgaaa caaatgcccg gtatggtgct gatcaaccgt atcctgcctg
     5401 gctttgaaaa ccgttgtatt gctcttgacg atcgttacgg tgcctggctg gcaacgcgtc
     5461 atttaattca gcaaggtcat acccgcattg gttatctgtg ctctaaccac tctatttctg
     5521 acgccgaaga tcgtctgcaa gggtattacg atgcccttgc tgaaagtggt attccggcca
     5581 atgatcggct ggtgacattt ggcgaaccag acgaaaacgg cggcgaacag gcaatgaccg
     5641 agcttttggg acgaggaaga aatttcactg cggtagcctg ttataacgat tcaatggcgg
     5701 cgggcgcgat gggcgttctc aatgataatg gtattgatgt accgggtgag atttcgttaa
     5761 ttggctttga tgatgtgctg gtgtcacgct atgtgcgtcc gcgcctgacc accgtgcgtt
     5821 acccaatcgt aacgatggcg acccaggctg ccgaactggc tttggcactg gcggataatc
     5881 gccctctccc ggaaattact aatgtattta gtccgacgct ggtacgtcgt cattcagtgt
     5941 caactccgtc gctggaggca agtcatcatg caaccagcga ctaaccgcag ttaaagcaat
     6001 tccagcgcca gtaattcttc gatggtctgg cgacggcgaa tcaaccgcgc ctgaccatta
     6061 tcaaacagaa cttctggtaa cagcggacgg ctattgtagt tggatgacat tgatgcgcca
     6121 tatgcccctg tatcatgcag taccagataa tcacctgcct tcacttccgg caaggcgcgg
     6181 gtttcaacat ttcccccttc ctgctgggta aagacatcgc ccgattcaca taacggcccg
     6241 gcgacgacgg tttccaccgt tggcgcgtgt tccagagaac gaccatcagc tgccagggca
     6301 ctgatatggt ggtaactacc gtacattgcc gggcgcatca gatcgttgaa cccggcatca
     6361 accagcacaa agtggcggct acccatttgt ttgacgctcc gcacctgagt aattaatacg
     6421 ccagactgcg ctaccaggaa gcgacccggt tcaatttcca gtttcacagg gtggcccaaa
     6481 tggcgagcga tttgctcacg cgcggcattc cacagaccat aataatgttc ggtatcaacc
     6541 gcctcttcac cctgttgata aggaatagaa agcccaccgc ccgcagaaat agcctgtaaa
     6601 tcctgaccga attcgatgac ctgacgcacc atagcaccac acacctgctc cagatgggca
     6661 taatcaacgc cagaaccaat gtgcatgtga atgccgacta gctgcagatg atgacgttgt
     6721 atcacgtcca gtgcggcggg cagatcggtg taccagatac cgtgcttgct gttttcgcca
     6781 ccggtattgg ttttttggct atgtccgtga ccaaaccccg gattaacgcg cagccatacc
     6841 cgatgccctg gcgaaacctg gcccagttgg tcgagcatat caacagaacc cgcattcacc
     6901 ggaatttgca attcactgac gcgttcaagc gtcgcctgat cgataacatc tgccgtaaaa
     6961 acaatatcat cggggtgcgt ttgcggattg taacccgccg ccaacgcacg ctctatttcg
     7021 cctaacgaga cggaatccac tttcacgccc tgctcacgca ttaagcgcaa aatatgaata
     7081 ttggaacagg ctttctgtgc aaagcgcacc acttcaaact gtttcagcgc tgctatctgc
     7141 cgacgaataa tttgcgcatc gtagacccac accgggcagc caaattcagc gggcaaacgc
     7201 agcagatttt cggcggtgag atcggtatcg gtgctgaaca gtgaatgtgg cataacaaac
     7261 tccagatgag tgctttttta tgattacgcc acatcataaa aagaataaaa aatatcgatt
     7321 tatgtcgaat ctatgcaaaa atgatatgga ttaccggatt gcgagagagc gctaatggcc
     7381 gccgttaact tacgtcatat tgaaattttt catgcggtaa tgaccgccgg aagcctgact
     7441 gaggcagcac acctgctaca cacctcacag ccaaccgtca gccgcgaact ggcgcgcttt
     7501 gagaaggtga tcgggctgaa attgtttgag cgcgtacgtg gacgattaca tcctaccgtg
     7561 caaggactgc gtctgtttga agaagtgcaa cgatcctggt acggactgga tcgcattgtc
     7621 agcgccgcag aaagtctgcg cgagtttcgc cagggagaac tgtctattgc ctgcctgccg
     7681 gtcttttcgc aatctttttt accgcagctc ctgcaaccct ttctggcacg ttatcccgat
     7741 gtcagcttaa atatcgtgcc ccaggaatca ccgctacttg aagagtggct ctcggcccag
     7801 cgtcatgatt taggactcac tgagacactc catacgcctg cgggaacaga acgtaccgaa
     7861 ttactctctt tagatgaagt gtgtgtgtta cctccgggtc atccgctggc ggtaaaaaag
     7921 gtattaacgc cggatgattt tcagggtgag aattacatca gcctttcccg tactgacagc
     7981 tatcgccagt tgctggatca gctatttact gaacatcagg ttaaacgacg catgatcgta
     8041 gaaacccaca gcgccgcgtc agtctgcgca atggtacggg cgggggtagg tgtttcggtg
     8101 gttaacccgc tcaccgcact agattatgcg gcaagcggtt tagtggtgcg gcggttcagt
     8161 attgcggttc cgttcaccgt cagcctgatc cgccccctgc accgcccgtc atcagcgctg
     8221 gttcaggcgt ttagtgggca tttacaagcg gggttaccga aactggtcac ttctcttgac
     8281 gctattttgt cgtcagctac gacagcataa aagcgacagc atcctcggca tggatcgacg
     8341 cggtatcaaa cacaggcaaa acactgcgct cttctggcac cagtaaacca atttctgtgc
     8401 agccaaaaat gacgccctgt gcgccctgtt ctgcaaggcg agcaatcact tgcgcataat
     8461 aagcgcgtga cgcttcggta aatttcccca gacacagttc ttcaaaaata atctgattaa
     8521 ttttcgcccg ttcatccgct tcaggaataa gacaattgat ggaaaattgt tccgtcagcc
     8581 gcccgcgata aaaatcctgt tccatggtgt aacgcgtacc cagcagcgcc acacgagtca
     8641 ttccggcccc ggtaattgca cgtccggtgg catccgcaat gtgtaaaaaa ggcagagtgc
     8701 aacgtgactc aatggcatcc gccactttat gcatcgtatt ggtacataac acaatacctt
     8761 ctgcgcccgc ccgctgtaag ccaagcgccg cctcagccag aatgtccccg gttttatccc
     8821 attccccgcg acgctggcac tcttctattt catgaaaatc gacgctatgt agcagcattt
     8881 gcgcagagtg aagcccacca agccgctgtt taatgccttc atttatcaga cgatagtaag
     8941 gaatggtgga ttcccagctc attcctccca gcaaaccaat tgttttcatt cctctctcct
     9001 gatgtgtgtt accgcaacaa ggctgaataa ggaacgagac aaacgcctca acggccaggt
     9061 gcccaatctc tattaacgaa aaaagggccg gatgtacagc acatccggcc cgtgaaatca
     9121 gacgccgata tttctcaact tctcgcctgc catcagtttg cgttcgatat gttccagcgt
     9181 gacatttttg gtttccggaa tgagccagaa agtaatgccc acaaacgcaa tgttcaacgc
     9241 agtgtagagc cagaacgtac cggcagcgcc aatgctatca agcagtgtca ggaaggccgc
     9301 gccgataatc atattcgaca cccagtttgt cgtcgtcgaa caggtaatac cgaaatcgcg
     9361 gcatttcagc ggctgaattt cagagcacag gatccacacc actggcgcgg cgctcatcgc
     9421 ataaccggca atacacatca tcgtcatgcc aacagagagc caggacaagc cactggaagc
     9481 cgtaccgtta tcaaactgca tcaggcaata gcccagcacc agagtgccta acgccatcac
     9541 gctgaaacca attttcagag ccggtttacg acctgcttta tctaccgtaa agaccgcaat
     9601 gaaggtcgcg aacataaagg tcagtcccac gaccagagtc gcaatcatct gttgttctgt
     9661 ggtcgtaaag cccgccattt tgaagatacg cggcgcgtag tacatgatga tgttcatacc
     9721 ggtaaactgc tgcatcgcct gcaacaacat accgagaaac acagcacgac ggacgttacg
     9781 gttgatctta aacagtgccc aaccgccctg ttttaacttc aggctttcac gaatttcgtt
     9841 gagttcttct cgcgcttttt ccgacgtatc gcgcagcata cgcaacactt cttccgcctc
     9901 aatatgacgc cccttttccg ccagccagcg cgggctattt ggcaggaaga ccaccagaat
     9961 aatcagcaga actgctggta aagcaagaac cccaacattg cgcgccagtt accgctataa
    10021 ctgaacgctg tatcggataa aaacgccagc acgatgccga gtgtgaccat caactggtac
    10081 atactgatca tcttaccgcg aacgtacttg ccatttcaga aagatacaga ggagcggtgt
    10141 aagacgcgat cccgacagca atgcccagca ccacacgagc ggcgattaac atctctacgc
    10201 tggtcgcaaa agcggaccct atagaaccga gtacaaacag gatggcccca ggcggaacga
    10261 cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac taaccaccca
    10321 ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg ctccggcgat
    10381 tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg aaacaaacat
    10441 attcatacgc cgcgtatcac gcaaagaacg tggcgttaaa gcagattccg tattgatagt
    10501 aaccattttt tcctgccagc agagagtaag acattgtgaa aaaatacgtg aacaattcac
    10561 acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcgaca tgccgttatg
    10621 tgatggatat tccaatttta aaattaagtt gaattattga gattattatt aaccacctaa
    10681 ttttaaagta gataaaattc ataaagttca ttaattgata attgatatgg attatttcat
    10741 aaccatgata tggattatga tgatctagag atataaaaaa ccctgccatg cggcagggtc
    10801 ataaaagtaa gaggaatgaa ttaacgcgcc agccaaccgc catccacagc aatggtataa
    10861 ccatttacat aatctgaagc gctggaggca agaaacacta ccggccccat caggtcactc
    10921 ggcagtcccc aacggccagc tggaatgcga tcgagaattt ccgcgctacg ttgttcatct
    10981 gcccgcagtt gttgggtatt gttggtcgcc atgtaacccg gagctatcgc attaacatta
    11041 atgttgtgtt tagcccattc gtttgccatc aatcgcgtca cacccatcac tccgcttttt
    11101 gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga cgcgatattg
    11161 ataatcttgc cgccattgcc ttgcgcgata aagtgtttcg ccgctgcctg agacatgaag
    11221 aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct gaactcgaga
    11281 gcgtcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg accaaattca
    11341 gctaccgcgc gatccagcag tccaggaatg ccatcaatct ttcgcagatc ggcggtcagg
    11401 cttaaaaaac gacgccccag cgccgtgacc tgctcgatgg tttcagtcgg ttcaacgatg
    11461 ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc ctggcccagc
    11521 ccagtatcac aaccagtgac gaccgcaact ttaccttgga gagaaaatgc acttaaaatc
    11581 ataacaacac cttattcgtt tatgcccaca actagcgcaa atctttaacg gccacatggt
    11641 ccatatcatt aaagacctgg ttttcaccga ccatgcccca gataaaggta taagctttgg
    11701 ttccgacgcc ggaatggatc gaccagctcg gggagatcac cgcctgctcg ttatgcatca
    11761 caatgtgacg cgtttcttgc ggctgcccca tcatgtggaa aacgcaggcg tcatcatcca
    11821 tattgaaata gaaataaact tccatccggc gctcgtgggt gtgacacggc atggtgttcc
    11881 acaagttacc tggagccagc tccgtcagcc ccatactcaa ttgacaggtt tccagtacat
    11941 ccgggacaaa atatttgtta atcgtgcgac ggttactggt gaggttatcg cctaacgtga
    12001 ctggagatac ttcgtccggt gtgacttttt tggtgggata cgtcgtatgc gcgggtgcgc
    12061 aattgtaata aaacttcgcc ggagtggcgg tatcaatact ggcaaagaca acttcttttg
    12121 cacctttacc aacatacagg gcgtcgcggt gaccgatttc atagcattga ccatcgacag
    12181 taatcgtacc ggcaccgcca atattgataa cacctaactc gcgacgttca aggaaatagc
    12241 ttacgccgag ttgtttacca acttccccgc caacggaaac cgttttagtt atcggcataa
    12301 tgccgccaac aataattcgg tcaatgtggc tgtaaaccat ggtgtactca tcggcgacaa
    12361 ataccttttc aaccaaaaat tcattacgca gcccttgggt atccagcgtt tttgcgtgcg
    12421 caccgtggat gctctgtctt acgtccacat caacctccga taatcagtga agcgtgaaaa
    12481 tagaaaacga tagtgaaaca ctgtttcgtt ttctctataa agggatgata tgcccatggg
    12541 ctagggtttt caattaaaaa taaaacaatg ttttattttt aatttaagga ttaaaaaaag
    12601 tgcagtgtcg atcacgaata tgtcatgaga atggaaaaat gagggagccc agaaaatgta
    12661 tgtcgacttg ccagccatca ttattcaaca gattaatgac ggcagatatt tatcggtaag
    12721 gaggaaatgc agagctactc gtcacgttca atggtcaatg ccacgcccta gcccccgccg
    12781 atacaaagcg ttgccagccc tttgcgggca ttacgtttca ccatttcatg aaccagagaa
    12841 accaggattc ggcaaccgga agcgcctatc gggtgaccga gtgcgatcgc gccaccattg
    12901 acattgaccc gacgctcatc ccactcaagc atcttgccaa ccgaaagcgc ctgtgcagca
    12961 aacgcttcat tagcctcgat aagatcgaca tcagccaact gccagcctac acgctccagg
    13021 caacggcggg tcgcatacac cggcgcaatt cccatcaatg ccggatctac accaacgctg
    13081 gcaaatgcgc ggatgcgggc cagcacgggt aaattcaacg ctcgtgcttt ggcttcgctc
    13141 atcatcatta ccgcagctgc gccatcgttt atggatgatg cattccccgc ggtcacagaa
    13201 ccgagactat caaatgaagg atttaaacgg gctaaacctt ctgcactggc gtcagtgcgt
    13261 ggctgttcat cggtatcaac aaccaacgtc tgaccgttac tttggatcat taccgggacg
    13321 atctcatctt taaatcgtcc ggcgtcaatc gccgctcgcg ctttttgttg cgagctaagt
    13381 gcgtaagcat cctgcaactg acggctgatg ccatattcgc gagccagatt ttcggcggtg
    13441 acaccaatat gataatcatt gaaggcatcc cacaacccat catgcaccag actgtcaacc
    13501 aactggctat tgccaagctg tgcaccggtg cggctatcag tcagaacatg tggtgcgcgg
    13561 ctcatgtttt cctggccacc ggcgatgaca atatcagcct cgccacactg tatcgcctga
    13621 gtagccagat gcagtgcttt aagaccggaa ccgcaaacgt cattaatcgt gattgcagaa
    13681 acgctgttag gaagaccacc tttaatagcc gattgccttg ccggattctg ccctgcccct
    13741 gcagtcaaca cctgaccaag aattacttca tccaccgcat atgcaggaac gccggtacgt
    13801 tctattaacg ctttcacgac cagactacca agttccacgg cggaatgacc cgctaacgca
    13861 ccacgaaagc agccgatagg tgtccgtaac gccccgacaa ccacaacgtc tttcatcaca
    13921 acctcgcacc aattaacagt gcaatagtaa atgattgtta attacagtta tcttaattgt
    13981 ttaaaaaaag tgatttttat cacaaaggaa atatgcctga gcagtagtca gagtcataac
    14041 tggcacgtaa ggtttgcaac cactaaccca ccaatagatg ggtagatagg gcgttaatct
    14101 cccatactta acttggttta tggtaaattg ccctccattt tgtttaattt gtagatgata
    14161 cgttcagata atgtctaata tttggtctaa agaagaaact ctgtggagtt tcgcgctcta
    14221 cggcacagcc gttggtgcag gcacgctatt ccttcctatt cagttaggtt cggcaggggc
    14281 tgtggtcctg tttattactg ctctggtcgc ctggccttta acatattagc cacataaagc
    14341 cttatgccag ttcatcctct catcaaaaac atcagcaggt gaagggataa cgggcgcggt
    14401 aacacactac tatggcaaga agattggtaa tctgattacc acgctgtact tcatcgcctt
    14461 ttttgtcgtc gtgttgatat atgcagtggc aattaccaac tcacttacgg aacagctggc
    14521 aaagcatatg gttattgatc ttcgcatccg tatgttggtg agtctgggtg ttgtattaat
    14581 tctgaatctc atttttctga tgggacgtca tgccactatt cgggtaatgg gatttttggt
    14641 attcccattg attgcctatt tcttatttct ttccatt
//
LOCUS       DAJVQH010000102        14624 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJVQH010000102 DAJVQH010000000
VERSION     DAJVQH010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.102"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..211)
                     /locus_tag="OQM87_003065"
     CDS             complement(<1..211)
                     /locus_tag="OQM87_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1509122.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQW"
     gene            219..719
                     /gene="tnpA"
                     /locus_tag="OQM87_003066"
     CDS             219..719
                     /gene="tnpA"
                     /locus_tag="OQM87_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCS1509123.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(758..1105)
                     /gene="grxD"
                     /locus_tag="OQM87_003067"
     CDS             complement(758..1105)
                     /gene="grxD"
                     /locus_tag="OQM87_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCS1509124.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1440..2267
                     /gene="mepH"
                     /locus_tag="OQM87_003068"
     CDS             1440..2267
                     /gene="mepH"
                     /locus_tag="OQM87_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCS1509125.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2395..2976
                     /gene="sodB"
                     /locus_tag="OQM87_003069"
     CDS             2395..2976
                     /gene="sodB"
                     /locus_tag="OQM87_003069"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCS1509126.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(3122..4291)
                     /locus_tag="OQM87_003070"
     CDS             complement(3122..4291)
                     /locus_tag="OQM87_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1509127.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4457..4546)
                     /gene="ynhF"
                     /locus_tag="OQM87_003071"
     CDS             complement(4457..4546)
                     /gene="ynhF"
                     /locus_tag="OQM87_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCS1509128.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            4845..5870
                     /gene="purR"
                     /locus_tag="OQM87_003072"
     CDS             4845..5870
                     /gene="purR"
                     /locus_tag="OQM87_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCS1509129.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5867..6799)
                     /locus_tag="OQM87_003073"
     CDS             complement(5867..6799)
                     /locus_tag="OQM87_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1509130.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            6912..8123
                     /locus_tag="OQM87_003074"
     CDS             6912..8123
                     /locus_tag="OQM87_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCS1509131.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8414..9562
                     /gene="cfa"
                     /locus_tag="OQM87_003075"
     CDS             8414..9562
                     /gene="cfa"
                     /locus_tag="OQM87_003075"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCS1509132.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9602..10243)
                     /gene="ribE"
                     /locus_tag="OQM87_003076"
     CDS             complement(9602..10243)
                     /gene="ribE"
                     /locus_tag="OQM87_003076"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCS1509133.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10458..11831
                     /gene="mdtK"
                     /locus_tag="OQM87_003077"
     CDS             10458..11831
                     /gene="mdtK"
                     /locus_tag="OQM87_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCS1509134.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11872..13128)
                     /locus_tag="OQM87_003078"
     CDS             complement(11872..13128)
                     /locus_tag="OQM87_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS1509135.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13436..13512
                     /locus_tag="OQM87_003079"
     tRNA            13436..13512
                     /locus_tag="OQM87_003079"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13470..13472,aa:Val,seq:gac)
     gene            13517..13593
                     /locus_tag="OQM87_003080"
     tRNA            13517..13593
                     /locus_tag="OQM87_003080"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13551..13553,aa:Val,seq:gac)
     gene            13701..14006
                     /locus_tag="OQM87_003081"
     CDS             13701..14006
                     /locus_tag="OQM87_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCS1509136.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            14132..>14624
                     /locus_tag="OQM87_003082"
     CDS             14132..>14624
                     /locus_tag="OQM87_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCS1509137.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATDH"
BASE COUNT         3594 a         3767 c         3463 g         3800 t
ORIGIN      
        1 gccattgcgt ttcagtggcg tttttccact caaccagcca ggaagccatt ttcccgtaag
       61 ggatgtattt atttccggcc tcatgattct cattctgaag cgccagagca cgattgaaaa
      121 cgaaacgaca tgcgcctgcg aagcgcctca tttcacgctc ctgttgacca ccgggtctta
      181 actggaattt aaatgcctgt aatcgtttca caggtcaaat gatactttgg tctgtgaaaa
      241 aagaaaccga tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt cacctggtct
      301 ttgtcaccag ataccgacgc cagatttttg accatgacac gacagaaaaa ctacgcactt
      361 acttttcaaa tgtatgtgct gattttgcag ctgaactggt tgaaatggat ggcgaaccag
      421 atcacgtcca tttgttaatt aactatcctc ccaaactggc gatatccagt ctggtaaaca
      481 gcctcaaagg catatcgaat aggttactgc gacgagatcg accagatatt gcagtcaggt
      541 attactacaa aggcgttttg tggagtcctg gctattttgc cagtagctgc gaaggtgcgc
      601 caatatccgt catccgccaa tacattgaac aacagcaaac acctggtcag gtggaaaacc
      661 gcgccttata tccccgccct gaaggacgag gttttacggc gcaccggata aaggactgac
      721 ggatgcccgt tcgcatccgt cagtattgca ggacggatta ttccgcgtcc ggctcttcag
      781 acttgtattt agcggcagtt tctttgatca gctgctgcag ttcgccacgc tgatacattt
      841 cgatcacgat atcacaaccg ccgaccagct cgccgtcaac ccacagttgc gggaaggtcg
      901 gccagttagc atatttcggc agttccgcac gaatgtccgg attctgcaga atatcaacat
      961 aggcaaaacg ttcgccacat gcggcaagcg cctggactgc ctgggcagag aaaccgcagc
     1021 tcggcagttt cggtgaacct ttcatgtaca gcaggatcgg gttttcagca atctggcgtt
     1081 ggattttttc gatagtggtg ctcattgtct tgcttcctca aacttcttta cggcagtaat
     1141 ctgacattgt agcgggtcag tgcggcatcg gaaaataaca ttttcatcac gcttttgcta
     1201 ttttatccct ttgctctatt tgttgcattt caaatattcg tttcttctct gatgcgcggc
     1261 aaactggcgt atttataacc atttttagct ttcactgctg catttttttg cgctcgccaa
     1321 cgaaacgtat tttttaacaa taaaagctat taactttctc ttcttctatg cattagaatc
     1381 atcaagtttt gtaaatcaga cgcaggcatg atagacctgc cttaacagag ggacgctcag
     1441 tggcgcggat aaaccgtatt tcgatcacgc tctgtgcttt actttttacc accctgcctt
     1501 taacgcctat ggcccatgct tcaaagcaag ccagggagag ttctgctacc gctcatatca
     1561 ccaaaaaagc agataaaaag aaaagcacgg caaccaccaa aaagacccag aaagccagcc
     1621 agaaaacagc gaaaaaagcc gccagtaaaa gtacgaccaa aagcaaaacc gcttcttccg
     1681 ttaaaaaatc ttccattacc gcttctaaaa acgccaaaac tcgcagcaaa cacgccgtca
     1741 ataaaacggc ctcagccagc tttaccgaaa agtgtaccaa acgtaagggc tataaatcgc
     1801 attgtgtgaa agtcaaaaat gccgcgtcag gaacccttgc cgacgcgcac aaagcgaagg
     1861 tgcaaaaagc cacgaaagtg gcaatgaata aactgatgca gcaaattggt aagccatatc
     1921 gttggggcgg cagctcaccg cgtaccggtt ttgattgcag cggcctggtt tattacgctt
     1981 ataaagattt ggtgaaaatt cgtattccgc gtacggcgaa tgaaatgtat cacctgcgtg
     2041 atgcaggacc tatcgaacgt agtgagctga aaaacggcga cctggtcttt ttccgtactc
     2101 agggacgcgg tacagccgat catgtcggcg tgtatgtcgg caacggcaaa tttattcagt
     2161 caccgcgcac aggtcaggaa attcaaatca cttctctcag tgaagactac tggcagcgcc
     2221 actatgttgg cgctcgtcgg gtaatgaccc caaaaacact tcgctaaaac tttaccctgt
     2281 tgttacggca acagggtaag ttcatctttt gtctcacctt ttaatttgct accctatcca
     2341 tacgcacaat aaggctattg tacgtatgca aattaataat aaaggagagt agcaatgtca
     2401 ttcgaattac ctgcactacc atatgctaaa gatgctctgg caccgcacat ttctgcggaa
     2461 actatcgagt atcactacgg caagcatcat cagacttatg tcactaacct gaacaacctg
     2521 attaaaggta ccgcgtttga aggtaaatca ctggaagaga ttattcgcag ctctgaaggt
     2581 ggcgtattca acaacgcagc tcaggtctgg aaccatactt tctactggaa ctgcctggca
     2641 ccgaacgccg gtggcgaacc gactggaaaa gtcgctgaag ctatcgccgc atcttttggc
     2701 agctttgccg atttcaaagc gcagtttact gatgcagcga tcaaaaactt tggttctggc
     2761 tggacctggc tggtgaaaaa cagcgatggc aaactggcta tcgtttcaac ctctaacgcg
     2821 ggtactccgc tgaccaccga tgcgactccg ctgctgaccg ttgatgtctg ggaacacgct
     2881 tattacatcg actatcgcaa tgcacgtcct ggctatctgg agcacttctg ggcgctggtg
     2941 aactgggaat tcgtagcgaa aaatctcgct gcataataac tgatggcaaa tgcagcattg
     3001 cctgatgcgc ttcgcttacc atgccaacgc ggatgaggcg ttaacagcgc attcggcaag
     3061 ataaatcgca cgttgtcagc aactgtaacg cagaaggtta tccttctgcg tttttgttta
     3121 attagctgtt agcaacgcaa actgtttcag gttgttttct ggctgacata aacaccagca
     3181 ataatgccag tcccgcgaca atcgcccccg tcaccggcac aaagctgtat cccagcccag
     3241 cggaaattac cgcaccacca gcagctgctc ccagcgcatt tccaagatta aaggcaccaa
     3301 tattgactga tgaagacaga cctggcgctt cactggcgac atgcatcacg cgcatctgta
     3361 acggaggtac aaccgcaaag gttgctgcgc cccacaccac catgctaata gctgcgccga
     3421 actcattgcg ggccaggaac gggattgcca gcataatcac catcagcagc aacaaaaagc
     3481 ctttcaacgt gccgttaact gaacgatctg ccagtttgcc gccgagatag ttaccgatag
     3541 agaatccgac accaatcagc accagcattg ccgtgacgaa caccggtgtt gcgtgggtaa
     3601 tactttgcag taccggagag atataggtgt agagggtaaa cattgcacca gctcccagta
     3661 ccgtcgtcag caatgcagac agcacctgcg gacgcattaa taccgccagc tcttttttca
     3721 cttcaggtcg tgcccctgta ccacctttag gtaatgagaa gaacagactt accattgaaa
     3781 tcactcccag ccccgccgtt gccagaaatg acatccgcca gccgatggtt tcacccaacc
     3841 aggtcgccgc cggcacgcca ccgatatttg ccagggttaa ccccataaac atagtggcaa
     3901 ctgcgctggc ctgtttatgt tttggcacca cgcttgcggc cacgactgaa cccaaaccaa
     3961 aaaatgctcc gtgattcagg ctggtcaaaa tacgtgaaag catcagggtc atataatccg
     4021 gcgcgatggc ggaaagaacg ttgcctagcg tgaaaattgc catcaggaaa atcaacgcac
     4081 tgcggcgggc acgatgagaa agtagaagcg tcatcagcgg cgcgcccacc attacgccaa
     4141 ctgcataggc actgattaac attccggcag cgggaatcga gacatccaca ccgcgcgcaa
     4201 tgacgggcaa caagcccatt ggcgagaact ccgttgtccc gataccaaac gcgccaatcg
     4261 ccagcgccag caacggatag ttaattttca tgccttatct ccacctcttc gcgtcattac
     4321 gcgatattca ttaaagtggc gaaagcatga cagcaatcac aaaaaaatga aaataacaaa
     4381 aagagaaaac acttttgcca ttttgctaac aaacaggaag gagatgcgag ggagaacgcg
     4441 ctccctcgag aggaaatcag tgcagcgcgg cagtcaaacc cacggctacg atcaaaccga
     4501 ggacgataat cgttgttacc agtgaaaatt taaggtcggt gctcatcaag ttttctcctt
     4561 ttttattacc acacaaaaag tgatattacg catttttaca cactgttatg aaaaaatctc
     4621 ccgtcattta taatgataag tgtttttacc acttcccctt ttcgtcaaga tcggccaaaa
     4681 ttccacgctt acactatttg cgtactggcc attgacccct tcctgacgct ccgtgtcgtt
     4741 tttccggcgt accgcaacac ttttgttgtg cgtaaggtgt gtaaaggcaa acgtttacct
     4801 tgcgattttg caggagctga agttagggtc tggagtgaaa tggaatggca acaataaaag
     4861 atgtagcgaa acgagcaaac gtttccacta caactgtgtc acacgtgatc aacaaaacac
     4921 gtttcgtcgc tgaagaaacg cgcaacgccg tgtgggcagc gattaaagaa ttacactact
     4981 cccctagcgc ggtggcgcgt agcctgaagg ttaaccacac caagtctatc ggtttgctgg
     5041 cgaccagcag cgaagcggcc tattttgccg agatcattga agcagttgaa aaaaattgct
     5101 tccagaaagg ttacaccctg attctgggta atgcgtggaa caatcttgag aaacagcggg
     5161 cttatctgtc gatgatggcg caaaaacgcg tcgatggtct gctggtgatg tgttctgagt
     5221 acccagagcc gttgctggcg atgctggaag agtatcgcca tatcccaatg gtggtgatgg
     5281 actggggtga agcaaaagct gacttcaccg atgcggtcat tgataacgcg ttcgaaggcg
     5341 gctacatggc cgggcgttat ctgattgaac gcggtcaccg cgaaatcggc gtcatccccg
     5401 gcccgctgga acgtaacacc ggcgcaggcc gccttgccgg ttttatgaag gcgatggaag
     5461 aagcgatgat caaggtgccg gaaagctgga ttgtgcaggg tgactttgaa cctgaatccg
     5521 gttatcgcgc catgcagcaa atcctgtcgc agccgcatcg ccctactgcc gtcttctgtg
     5581 gtggcgatat catggcaatg ggcgcacttt gtgctgctga tgaaatgggc ctgcgcgtcc
     5641 cgcaggatgt ttcgctgatc ggttatgata acgtgcgcaa cgcgcgctat tttacgccgg
     5701 cgctgaccac gatccatcag ccaaaagatt cgctgggtga aacagcgttc aacatgctgt
     5761 tggatcgtat cgtcaacaaa cgtgaagaac cgcagtccat tgaagtgcat ccgcgtttga
     5821 ttgaacgccg ctccgtggct gacggcccgt tccgcgacta tcgtcgttaa tcacccgttg
     5881 cgggagtctc ttccggctcc cgcagccact ccttattcag cgtctcacta tcgccgagat
     5941 actcaagcag ccaggttaac gcaggcgaca tatcattttg ctgccatgtc agacaacatg
     6001 ccgaatccgg gaaggggttt tccagttcaa gtgctaccca cttcccctca ttaagccacg
     6061 gtttggcgaa atgtgttggc accatcccta tgcataatcc tgccgagata caggttgccg
     6121 atgattccca gtcaggtacg acaagtcttt tttggttatc cagcaaccag gtaatacgtt
     6181 taggtagcgt tcgcgaggtg tcttcgcgca ccaacgacgg ccagttgcgc aacgtatcat
     6241 cgctgaacgg gccatccatc aacgccagcg ggtggtgact ggcaacaacg caactccagc
     6301 ttagcatccc catatcccgg aaggcataac gaccgcctac cggaatcgcg cgtgttgcgc
     6361 caattgccag ttccacgcgc ccgtcggaaa gcgcatccca gacaccgttg aacacttcct
     6421 gaaagacaag aagttcaaca tcatcaaaat ggcgataaaa atcaacgatc atctgccgtg
     6481 tacgttctgg cctgacaata ttatccactg cgatagctaa ctgaccgcgc cagccgttcg
     6541 ctatctgctg acattgctgg cgggtgatct gcattttttt gacaacagag cgcccttctt
     6601 tgagaaacca cgctccagca gcggtcagct ccacatcacg gtgccgtcgt tcaaagagcg
     6661 gtaccgccag ccactcttcc agctgacgca cggtatagct gaccgcagaa ggaacgcgat
     6721 gcagctcctg tgccgcagcg ctaaaactac cattacgcgc taccgcatca acaacttcga
     6781 gtgaatattc tgaccacata gtcagcctgc aaaatttttg aaaccagtca tcaaatatta
     6841 ccgtttcaca acactaattt cactccctac actttgcggc ggtgtttaat tgagagattt
     6901 agagaatata catgcaacct gggaaaagat ttttagtctg gctggcgggt ttgagcgtac
     6961 tcggttttct ggcaaccgat atgtatctgc ctgctttcgc cgccatacag gccgacctgc
     7021 aaacgcctgc gtctgctgtc agtgccagcc ttagtctgtt ccttgccggt tttgccgcag
     7081 cccagcttct gtgggggcca ctctccgacc gttatggtcg taaaccggtg ttattaatcg
     7141 gcctgacaat ttttgcgtta ggtagtctgg ggatgctgtg ggtagaaaac gccgccacgc
     7201 tgctggtatt gcgttttgta caggctgtgg gggtctgcgc cgcggcggtt atctggcaag
     7261 cattagtgac agattattat ccttcacaga aagtcaaccg tatttttgcg accatcatgc
     7321 cgctggtggg tctgtctccg gcactggctc ccctgttagg aagctggctg ctggtccatt
     7381 tttcctggca ggcgattttc gccaccctgt ttgccattac cgtggtgctg attctgccta
     7441 ttttctggct caaacccacg acgaaggccc gtaacaatag tcaggatggt ctgaccttta
     7501 ccgacctgct acgttctaaa acctatcgcg gcaacgtgct gatatacgca gcctgttcag
     7561 ccagtttttt tgcatggctg accggttcac cgttcatcct tagtgaaatg ggctacagcc
     7621 cggcagttat tggtttaagt tatgtcccgc aaactatcgc gtttctgatt ggtggttatg
     7681 gctgtcgcac cgcactgcag aaatggcaag gcaagcagtt attaccgtgg ttgctggtgc
     7741 tgtttgctgt cagcgtcatt gcgacctggg ctgcgggctt cattagccat gtgtcgctgg
     7801 tcgaaatcct gatcccattc tgtgtgatgg cgattgccaa tggcgcgatc taccctattg
     7861 ttgtcgccca ggcgctgcgt cccttcccac atgcaaccgg tcgcgccgca gcgttgcaga
     7921 acactctgca actgggtctg tgcttcctcg caagtctggt agtttcctgg cttattagta
     7981 tcagcacgcc attgctcacc accaccagcg tgatgttatc aacagtagtg ctggtcgcgc
     8041 tgggttacat gatgcaacgt tgtgaagaag ttgactgccc gaatcatggc aatgccgaag
     8101 tcgctcatag cgaatcacac tgatctatat cgatatactt atacttaggc tgctaacaaa
     8161 attttgttgt atgattgaaa ttagcggcct atactaattt cgagttgtta aagctacgat
     8221 aaatattatg tttttacggg gacaggatcg ttcccgactc actatggatg gtcatttcgg
     8281 caagggttcc tcctttccct ctgttctacg tcggattata gactcgcggt tttttctgcg
     8341 agatttctca caaagcccaa aaagcgtcta cgctgtttta aggttctgat caccgaccag
     8401 tgatggagaa actatgagtt catcgtgtat agaagaagtc agtgtaccgg atgacaactg
     8461 gtaccgtatc gccaacgaat tacttagccg tgccggtata gccattaacg gttctgcccc
     8521 ggcggatatt cgtgtgaaaa accccgattt ttttaaacgc gttctgcagg aaggctcttt
     8581 ggggttaggc gaaagttata tggatggctg gtgggaatgt gaccgactgg atatgttttt
     8641 tagcaaagtc ttacgcgcag gtctcgagaa ccaactcccc catcatttca aagacacgct
     8701 gcgtattgcc ggcgctcgtc tcttcaatct gcagagtaaa aaacgtgcct ggatagtcgg
     8761 caaggagcat tacgatttgg gtaatgactt gttcagccgc atgcttgatc ccttcatgca
     8821 atattcctgc gcttactgga aagatgccga taatctggaa tctgcccagc aggcgaagct
     8881 caaaatgatt tgtgaaaaat tgcagttaaa accagggatg cgcgtactgg atattggctg
     8941 cggctggggc ggactggcgc actacatggc atctaactat gacgtaagcg tggtcggcgt
     9001 caccatttct gccgaacagc aaaaaatggc tcaggaacgc tgtaaaggcc tggatgtcac
     9061 cattttgctg caagattatc gtgacctgaa cgaccagttt gatcgtattg tttctgtggg
     9121 gatgttcgag cacgtcggac cgaaaaatta cgatacctat tttgcggtgg tggatcgtaa
     9181 tttgaaaccg gaaggtatat tcctgctcca tactatcggt tcgaaaaaaa ccgatctgaa
     9241 tgttgatccc tggattaata aatatatttt tccgaacggt tgcctgccct ctgtacgcca
     9301 gattgctcaa tccagcgaac cccactttgt gatggaagac tggcataact tcggtgctga
     9361 ttacgatact acgttgatgg cgtggtatga acgattcctc gccgcatggc cagaaattgc
     9421 ggataactac agtgaacgct ttaaacggat gtttacctat tatctgaatg cctgcgcagg
     9481 tgctttccgc gcccgtgata ttcagctctg gcaggtggtg ttctcacgcg gtgttgaaaa
     9541 cggccttcga gtcgctcgct aaaggctatt ctatcgcccc ctcttcgggg gcgagttcag
     9601 atcaggcttc tgtacctggt tgattcatgg cattttctcg tgccgccagc acacgttcta
     9661 ccgtatctac cactgcctga gtttgtggat cgatttcaat gttgacgcgt gcgccaagtt
     9721 ttttcttccc aagagtcgtg cgttccagtg tttccggaat taaatggacg caaaaacgcg
     9781 ttggcgtgac ttcgccgacg gtcaggctaa taccgtcgat gccaataaat cctttgtaca
     9841 ggatatattt catcaactga ctatcctgga ctttaaacca gatctggcga ttattttccg
     9901 aggttaatat tttcgccact tcagcggtgg tcataatatg acctgacatt aagtgtccgc
     9961 caatttcatc actgaatttc gccgcacgct caacgtttac ccaatccccc acttttaaat
    10021 cgccaagatt ggtaatgcgt aacgtttctt tcatcaggtc aaaactgaca tggttgccgt
    10081 taatttccgt cacggtcagg cagcaaccgt tatgcgccac ggaagcaccg gtttccaggc
    10141 cgtccagcat gtggtcgggt aactccacca catgcgtacg aaaatttggt ttctcgtcaa
    10201 tcgacaccag ttttgcggtg ccctgtacaa tccccgtaaa catacttaca actcctgaaa
    10261 tcagttaaga cattctgttc agcacaatag caggtggaaa acgcccttac cagtgaaggg
    10321 gtaagaatgg ctattttttc actggagaat taataaatcc tcgctacaat agactgaatt
    10381 tcccctgctt cttctttttg ctgcccattc aggcggcttt ttagtctctc atataactac
    10441 aaataaaagg tgttcacgtg cagaagtata tcagtgaagc gcgtctgtta ttagcattag
    10501 caatcccggt gattctcgcg caaatcgccc aaactgcgat gggttttgtc gataccgtga
    10561 tggcgggcgg ctatagtgcc accgacatgg cggcggtcgc tatcggtact tctatctggc
    10621 ttccggcgat cctctttggt cacggactgc tgctggcatt aacgccggtt atcgcgcaat
    10681 taaatggttc cggtcgacgt gagcgcattg cgcatcaggt gcgacaaggt ttctggctgg
    10741 caggttttgt ttccgttctc attatgctgg tgctgtggaa tgcaggttac attatccgct
    10801 ccatggaaaa catcgatccg gctctggcgg acaaagccgt gggttatctg cgtgcgttgt
    10861 tgtggggcgc gccgggatat ctgttcttcc aggttgcccg taaccaatgt gaaggtctgg
    10921 caaaaaccaa gccgggtatg gtaatgggct ttatcggcct gctggtgaac atcccggtga
    10981 actatatctt tatttatggt catttcggta tgcctgagct cggtggcgtt ggttgtggcg
    11041 tggctactgc ggcggtgtat tgggtcatgt tccttgccat ggtttcttac attaaacgcg
    11101 cccgctccat gcgcgatatt cgtaacgaaa aaggcaccgc taagcccgat cctgcggtta
    11161 tgaaacgact gattcaactc ggtttgccga ttgcgctggc actgttcttt gaagtgacac
    11221 tgtttgccgt cgtggctctg ttagtgtctc cgctcggtat tgttgatgtc gcaggacacc
    11281 agattgccct gaactttagt tcactgatgt ttgtgcttcc aatgtcgctg gcggcagcgg
    11341 taactatccg cgtaggttat cgtctgggtc agggctcaac gctggatgcg caaaccgctg
    11401 cgcggaccgg gcttatggtg ggtgtctgta tggcaaccct gacggccatt ttcacggttt
    11461 cactgcggga gcaaatcgcc ctgttgtaca acgacaatcc cgaggttgta acgctggctg
    11521 cgcatttgat gttgctggcg gcggtatatc agatttctga ctcaatccag gtgattggca
    11581 gtgggatttt gcgtggttat aaagatacgc gttccatttt ctatatcacc tttacagctt
    11641 actgggtgct gggcttgcca agcggctata ttctggcact gaccgatctg gtcgttgaac
    11701 ctatggggcc agcaggcttc tggataggct ttattatcgg cctgacatcg gcagccatta
    11761 tgatgatgct gcgtatgcgg ttcctgcaac gtatgccgtc agccatcatt ctgcaacgag
    11821 cgtcccgcta ataaagacaa ggcgcaacct tcacgggttg cgcctgtgtt tttacgcagg
    11881 ctggagcgtt gcgccaatcc cgtcttcgtc tggctgtaat ttcagagcgt tacacagagt
    11941 ttgccgactg gcatctgtta tctcaacgcg tatatccccg ttaacttccc ctttcacatt
    12001 gatgaagtca ggtgaaattt ctttcgcact ggtttgcagg caaatcgctc cctctccgcg
    12061 caggcgtttc actttcagtt gcacatgcgt tgccgcacca gtgatcagcc acagttttcc
    12121 gccattcaga ttgatgttac caacaatgaa agtgacgctg tcacctttta ccgtactgcc
    12181 gtcgagaatc aacacaccgc cctgctgtac agtaatcgtt gattgttgca gctctgcgcc
    12241 ataaccaata atcattgaag cgccgtccag taatgcaaca ctcccggtca gttcaaccgg
    12301 tgcgcgatct tcctctacga caaaattttc gtccgcgctg gtgggtgaca acaggatcac
    12361 tgttccacct tgtattaccg cattttccag cgtaccggca tgcacttcag cccgatcccc
    12421 cacattcacc gacaggtttt gcgtcttacc ggaactgtag agctgaaggc catccgtccc
    12481 cagacgataa atggatccat tctcaacaat agtgtcagag tcttcaccac cgagattatt
    12541 catggtgcca ccagcaagca acgtatcact ggctttcccg gccagcataa aaacgccgcc
    12601 ctgattaatc tcacaattgc tggcttctcc acttggatac accaacaatt caccaccttc
    12661 atctaccacg accgctgagg ttgtcccatt aaccaacagg ccgccctctt gatcgagaat
    12721 gattttttcc gcgcgatgtc cttccagtac acgcaggtta ccgccatttt ccagcaacaa
    12781 accgtaggca taaccctgat caacgctgaa ttcaccttcg ggatggcggc cattaaccgt
    12841 agcgagcgta gagagactaa ttgcgcctcc ctcggactga acaatatctg tggcaatggc
    12901 accgtcgctg accgatagcg tgccattgtt accgtactgt gtgcatttgg cgatcccacc
    12961 ggttgccact ttcaaccagc cagcccccgt gaccacggta ttttcggctc ttccgccagc
    13021 ttcaacaatc aactcgcctt cagtaagttg cgtcgcgcca atgacctcgc cggtcttaac
    13081 aatttgcaca ttcccgtcgc tcatcaagtt tttcgcatca gatcccatga tttattcctt
    13141 tgctgcatct gtgtgccttt attgctacct aagtgtaaag gctacggagg atttatccac
    13201 gacagatttg agatggtggc aaacaactct gtttaaactc tgatacacga attattgggt
    13261 tgtatcagat gtaaatgcga tcctgaataa aaatcgccct tgcaaatcaa caaaatatag
    13321 ccaatttgct taaaagctct gcaaacggtc gaaatagcgc agaaaattac gttttgcctc
    13381 ttgccacctt tccactctgc cgctaatatt cgtccccgtt gtcacctaca acgttgcgtt
    13441 catagctcag ttggttagag caccaccttg acatggtggg ggtcgttggt tcgagtccaa
    13501 ttgaacgcac catcctgcgt ccgtagctca gttggttaga gcaccacctt gacatggtgg
    13561 gggtcggtgg ttcgagtcca ctcggacgca ccagattttc ttaatctggt cttttccttt
    13621 ttccctctgt ttcttctctg tatccaatac gttaaaagat ttacactgtc ttcgtatgcg
    13681 ttatcagaag gagaatcgct atggcaactt tgttacaatt tcattttgct tttaatggcc
    13741 catttggcga cgcaatggct gagcagctta aaccacttgc tgagtcgatt aatcaggaac
    13801 ctggttttct gtggaaggta tggacagaaa gtgaaaagaa ccacgaagcc ggtgggatct
    13861 accttttcac tgatgaaaaa agcgcccttg cctatctgga aaaacatact gcccgactga
    13921 aaaatctcgg cgttgaggaa gttgtcgcca aagttttcga tgtcaatgag ccacttagtc
    13981 aaatcaatca ggcaaaactc gcctgacaga atttaatcaa gggcggttag cgcccttttc
    14041 atccctgtct gaaatttctc aaattctaaa aatctcaacc aaacttatct gataacacta
    14101 aattcgaaag aatgcgtaca ggtaagtaac aatgaaaaaa attgctattg tgggtgccgg
    14161 gcctacgggg atctacacct tattctcgct tctacagcaa caaactccac tttctatttc
    14221 tatcttcgag caggctgacg aggccggtgt cgggatgcca tacagtgatg aggaaaactc
    14281 aaaaatgatg ctggcaaata ttgccagtat tgaaataccg ccgattaatt gtacgtatct
    14341 cgaatggcta caaaagcaag aagccagcca tctccagcgt tatggcgtta aaaaagaaac
    14401 cttgcacgat cgtcagtttt taccgcgaat tctgctgggc gaatatttcc gcgatcaatt
    14461 tttacgatta gtagaccagg cacgacagca aaaatttgca gtggctgttt atgaatcatg
    14521 ccaggttacc gatctgcaaa ttacaaatgc tggcgtcatg ctcgctacaa atcaggattt
    14581 acccagcgag acgtttgatt tagcggtgat tgccacggat cacg
//
LOCUS       DAJVQH010000103        14590 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJVQH010000103 DAJVQH010000000
VERSION     DAJVQH010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.103"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..227
                     /locus_tag="OQM87_003083"
     CDS             <1..227
                     /locus_tag="OQM87_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509138.1"
                     /translation="ADEQATQPDSVIAENYARVVAEVMRRDGIELNGVDMRNIRTRVL
                     ELLAYRRRVEMYREKEKITYHGKKPERLRR"
     gene            complement(1024..2484)
                     /gene="phoQ"
                     /locus_tag="OQM87_003084"
     CDS             complement(1024..2484)
                     /gene="phoQ"
                     /locus_tag="OQM87_003084"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCS1509139.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2484..3155)
                     /gene="phoP"
                     /locus_tag="OQM87_003085"
     CDS             complement(2484..3155)
                     /gene="phoP"
                     /locus_tag="OQM87_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCS1509140.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3324..4694)
                     /gene="purB"
                     /locus_tag="OQM87_003086"
     CDS             complement(3324..4694)
                     /gene="purB"
                     /locus_tag="OQM87_003086"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCS1509141.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4698..5339)
                     /gene="hflD"
                     /locus_tag="OQM87_003087"
     CDS             complement(4698..5339)
                     /gene="hflD"
                     /locus_tag="OQM87_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCS1509142.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5375..6481)
                     /gene="mnmA"
                     /locus_tag="OQM87_003088"
     CDS             complement(5375..6481)
                     /gene="mnmA"
                     /locus_tag="OQM87_003088"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCS1509143.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGHEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6535..6996)
                     /gene="nudJ"
                     /locus_tag="OQM87_003089"
     CDS             complement(6535..6996)
                     /gene="nudJ"
                     /locus_tag="OQM87_003089"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCS1509144.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(7006..7659)
                     /gene="rluE"
                     /locus_tag="OQM87_003090"
     CDS             complement(7006..7659)
                     /gene="rluE"
                     /locus_tag="OQM87_003090"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCS1509145.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7831..9081
                     /gene="icd"
                     /locus_tag="OQM87_003091"
     CDS             7831..9081
                     /gene="icd"
                     /locus_tag="OQM87_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCS1509146.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(9184..9507)
                     /gene="iraM"
                     /locus_tag="OQM87_003092"
     CDS             complement(9184..9507)
                     /gene="iraM"
                     /locus_tag="OQM87_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1509147.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
     gene            complement(9760..9804)
                     /locus_tag="OQM87_003093"
     CDS             complement(9760..9804)
                     /locus_tag="OQM87_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509148.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            complement(10040..10150)
                     /locus_tag="OQM87_003094"
     CDS             complement(10040..10150)
                     /locus_tag="OQM87_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509149.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
     gene            complement(10203..10607)
                     /locus_tag="OQM87_003095"
     CDS             complement(10203..10607)
                     /locus_tag="OQM87_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCS1509150.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(10828..11559)
                     /gene="bluR"
                     /locus_tag="OQM87_003096"
     CDS             complement(10828..11559)
                     /gene="bluR"
                     /locus_tag="OQM87_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCS1509151.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(11764..12975)
                     /gene="bluF"
                     /locus_tag="OQM87_003097"
     CDS             complement(11764..12975)
                     /gene="bluF"
                     /locus_tag="OQM87_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCS1509152.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            13289..13525
                     /locus_tag="OQM87_003098"
     CDS             13289..13525
                     /locus_tag="OQM87_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCS1509153.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            13568..13840
                     /locus_tag="OQM87_003099"
     CDS             13568..13840
                     /locus_tag="OQM87_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCS1509154.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            13869..14135
                     /gene="ariR"
                     /locus_tag="OQM87_003100"
     CDS             13869..14135
                     /gene="ariR"
                     /locus_tag="OQM87_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCS1509155.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            14248..14496
                     /locus_tag="OQM87_003101"
     CDS             14248..14496
                     /locus_tag="OQM87_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509156.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         3913 a         3328 c         3397 g         3952 t
ORIGIN      
        1 aggctgacga acaggccacg cagccggaca gcgtgatagc cgaaaactat gcgcgtgttg
       61 ttgctgaggt gatgcgccgt gatggtatag aacttaatgg cgtggatatg cgcaacatac
      121 gaaccagagt tcttgagttg ctggcctacc gtcgccgcgt ggagatgtat cgggagaagg
      181 agaaaatcac gtaccacggg aagaagccgg agcggttacg gcggtaactc actgagatta
      241 aagcaaagcg caaaattgcg ctggctacat ccctcacatc tgaggactgt taaccagcag
      301 gccggaatat attcagaaac tacgaaattt tcgcagttta attactcgtc agcttaatcg
      361 ctaacccgct ctgatatttt cggaaacctc aatttgagga agtcggcgag gtaactgttt
      421 gatattcccg atagcgcaaa aatgcgcggt cgggcagaat gaccaccacc tcaatcgtga
      481 cggagagtga caaccagtga cacaccgtga caaaaagacg gtttaaccgt tcattaaaaa
      541 tcgacgtgat tttgtgcttt ccggatgacg tggccaacgt cattttttag cgaaaaattc
      601 tgattaaggt cactggctga acggtcataa tgaccacgca gaccatcgag gcaaccatag
      661 cgatcatcgt tacggttgga gcttttccgc cattggtgga aaaggtgcac atctggatca
      721 tcattacggt gatcaagggt atgtaaaacc caccagcctg attaaaggtt aaccggaaaa
      781 aaaatccggt agccccgtta tggggatccc catatcgaca ttaacgcccc tcatgaattg
      841 gtgcgcttcc ccctggaaag attacccgcc tgtatatttc ttgtgtctat ttgttccacg
      901 ttgtttcata cagtgcaccg aacggtgtag ttaatggtgt agtcaatttg cgatttttgg
      961 cactttttca atgaaaattg tttaatcgaa tcaatgactt gatgtagtgg taaaaggaca
     1021 tatttattca tctttcggcg cagaatgctg gcgaccaaaa atcacctcca tccgcgcacc
     1081 gcccagcatg ctctctccgg cgacgatttt accctcatat tgctcggtga tttcgcgggc
     1141 taccgccagc cctacacctt gcccagggcg taaagtatca acccgttgac cacggtcgaa
     1201 aatgacctct cgcttgctta atggaatacc ggggccatca tcctcgacca caatatagag
     1261 atgctcgtcg gtttgccttg cagaaatttc gacaaactcg aggcaatatt tacaggcatt
     1321 atccagcacg ttgcccatca cctcgacaaa atcgttctgc tcaccgacaa agctgatctc
     1381 tggcgaaata tcgagagaga tattgacccc tttgcgttga tacactttgt tcagcgctga
     1441 ggtgagattg tccagcagtg gggcgaccgg atgcagctcg cggctgagca atgtcccgcc
     1501 gcgcatactg gcacgatgca ggtagtagcc aatttgctgt gaaatgcggc tgatttgctc
     1561 cagcattacc ggctcagcat cactgacgct catcttttca ctacgcagag aacgcagcgt
     1621 actttgcagc accgccagtg gcgttttcag actatgggtc aggtcggtga gcgtcgtacg
     1681 gtatttgtcg taacgttcgc gttcactttt taacaatcgg ttcaggtttc gtaccagact
     1741 ggtcagttct cgcgttgtgg ctggattgag caattcgcgg ttatgttctt ccagttcgcg
     1801 gacttctttt gccagggctt cgatggggcg taaactccac caggcggcga cccacagcag
     1861 cgggatcact aacagcagat tggctgagag tacatagata aaccagctcc agaccatata
     1921 ggaacttttt agctccaccg gaatggtatc caccaccaca atggttaatt ttggcatccg
     1981 cgatgttgcc gggtagacgt ttaccgccac cgagtgggtc atctccgcgt cgtcatcatc
     2041 ttcccgcact tcctgcaact gttgctgtat cgaatgatct ccactcagca agaggctggt
     2101 atcgttaaca tccgcttcaa tttcatgaaa accattcgat ttcagccagt caggctggat
     2161 cattttcatc agccagggca cgtcacgttg cgcccataaa agctgcccgt tctcatcata
     2221 aattagcgtc atggtggggc tttgcttgtc gatattttcg ggtaactcga catgcaactt
     2281 attgttttcc cactgcgcaa gggtatagaa cagattgctc tcgccacgta acagccgaaa
     2341 cgtagtttta tcgaaactga cgctataacc gatcagcgcg accattccgt aggcaagcga
     2401 aagcaccagt actaccgctg ccgttgccaa cagaaaacgt acccgcagcg agagcgggaa
     2461 aaaaagacgc agtaattttt tcatcagcgc aattcgaaca ggtagccctg gccgcgaacg
     2521 gtggtaatga cttcttgcgg atattgtgcc tgaatttttt tgcgcagacg tcccatcagt
     2581 acatcaatgg tatggctttc ccgcagctcc gcatccggat agagttgtag cattaacgaa
     2641 tctttgctga ccactttgcc attattgcgt atcaacgttt ccataatggt gtattcgaac
     2701 gcggtcagtt tgatcacttc gtcattaata gataattcac ggcgagagag atcaacctga
     2761 aacgggggga gcgaaatgac ctgtgaagcc agaccgctat tacgccgcat taatgcctgc
     2821 attcgcgcca tcacctcttc aatatgaaac ggtttagtca cgtaatcatc agcaccggca
     2881 cttaatactt cgactttgtc ctgccagctt tcacgggcgg ttaataccag aatcggcagt
     2941 gaaacatcgt tgctacgcca gcggcgaatc agtgacagac cgtcctcgtc aggcaatccg
     3001 agatcgacaa tcgcaatatc cggtaaatgt tcattgagat aataatcggc ttctttggca
     3061 tcttctgcgt catcgacctg atgaccagca tcctgaatct gaactttaag gtggtgacgt
     3121 aacaacgcat tgtcttcaac aaccagtacg cgcattttta tttctccctg tcttaattat
     3181 taaaatagtg taacgcgatt atgtgtttat gggggtaaac attaaataaa ccagcgggga
     3241 ggggaggtaa agtgaaaaaa gaaaaagtgg ataatcttaa taagcaggcc ggacagcatc
     3301 gccatccggc actgatttga ggcttatttc agctcatcaa ccatcgtgat agctcgacca
     3361 atatagttag ccggcgtcat cgctttcagg cgggctttct cttcttctgg caacgccaga
     3421 ccatcgataa actgcttcat gccttcggcg tcaacgcgct taccgcgagt aagctctttc
     3481 agcttctcgt acggtttttc gatgccatag cgacgcataa ctgtctggat tggttctgcc
     3541 agcacttccc agttgtgatc cagctcatcc agcagatggt cacggttcac ttccagtttg
     3601 ctcacgcctt tcagggtgga ttgatacgca atcagcgcat aaccgatacc cacgccgagg
     3661 ttacgcagca cggtggagtc ggtcaggtca cgctgccagc gggaaaccgg cagtttgctt
     3721 gccagatgct gcaataccgc gttggaaagg cccagattcc cttcggagtt ttcgaagtcg
     3781 atcgggttaa ctttatgcgg catggtggaa gaaccaatct caccagcaat ggttttctgt
     3841 ttgaagtggt taagggcgat ataaccccag acgtcacggt caaagtcgat cagaatggtg
     3901 ttgaagcgcg caacgcaatc aaacagttcg gcaatgtagt cgtgcggttc aatctgggta
     3961 gtgtacgggt tccattgaat acccagcgag gtgacgaact cttcgctgaa ctgatgccag
     4021 tcaacttccg ggtaagcggc gatgtgggcg ttatagttac cgaccgcgcc gttgattttg
     4081 ccgaggatct ccacctggtt aagctggcgg tgctggcgct ccatacggta ggcgacgttt
     4141 gccatctctt taccgatggt tgacggcgtg gctggctgac cgtgggtacg agacagcagc
     4201 gggatatcgc ggtactgaac ggcgagatct ttaatgccat caatcagttg acgccagtac
     4261 ggcaggatca cttcatcacg cgcggttttc agcattaatg cgtgggagag gttattgata
     4321 tcttccgaag tacaggcaaa gtggatgaat tcagaaaccg cgtgcagttc cgggatctcc
     4381 gccacttttt ctttcaggaa atactcaacc gctttaacgt cgtggttagt ggtacgctcg
     4441 atggttttga tgcgtgcggc atcttcttcg ctgaaattgg cgacaattgc atcaaggtaa
     4501 ccgattgcgt cggcagcaaa agcaggaact tccttgatcg ctgcgtgcgc ggccagtttt
     4561 tgcagccaac gtacttcaac ttgtacacgg aatttcagca aaccatattc gctgaaaatc
     4621 ccgcgcagcg cgctgagttt atcgccgtag cgtccatcga caggggaaac ggcggtcagt
     4681 gaggataatt ccatagatca caactccggg gttaaatgag caagaatttg ttttgcctga
     4741 gtggtcaggc gattacgaga aaacatcagt tgtagacgtc cgccgccgac ctggtgccag
     4801 agcacggcgg cgcgaatgcc tgccagcagg gttgcgcgaa ctttcgcctg cacttgtggg
     4861 ctttgcagta cagcagggga accggtgacc tgaatgcgcg ggccaagcgg gctgatcaca
     4921 tcaacataga tagcagccat cgcgctcatc agcgtttcgg actgtaaatc gaagtgttcg
     4981 agctggcgtt gcaggccgtt gatccggttg cccagagtgt cgagcgcgcc tttcgctgag
     5041 gagagtttgc gctcaagcac catcaagctg agtgtgtagc gggttaattc ggcgtttaag
     5101 ccctggcaac tgctggcatt gagcacgccg agcagggttt ccagcccgac gcgcaggttg
     5161 gcttcgctac cgccaaaaac cgccagcgtc gagctggggt tcatatcaat aatactgttg
     5221 agtgagacgt gtagcgcatc ggcatcacaa tgcccctggt gagcgagttg ttgcaccagg
     5281 cgtgccgact gacaaatacc ggccagggcg agggtgatgt catagtaatt ctttgccacg
     5341 ttcactgctt ccttgtttta agtaaagata ataatcagac cggcagcggc agacgctgct
     5401 caataatacc gccaccgagg cacacttcac cgttatagaa gacggcagac tggcccggcg
     5461 tcacggcggc aaccggctca tcgaaaatca ctttaatgcg atcgtcgtcc agcgccttga
     5521 cggtgcacgg aatgtcggtc tggcgatagc gggtttttac cgtgcaacgc atagtgccgg
     5581 tgaatggttc gcgatcgacc cagtgcagct gctgggcaat caacccgaca gacatcagcc
     5641 gcgggtgttc atggccctga gcgacaacca gaatgttgtt ttcgacgtct ttgtccacca
     5701 cataccacgg ttcttcggtg ccttctttgg tgccaccgat acccagacct ttacgctgac
     5761 cgagagtgtg atacatcagc ccctggtgct caccaatttc atggccatcg acggtaatga
     5821 ttttgcccgg ttgcgccggg agataacggc ccaggaactc gcggaattta cgttcgccaa
     5881 tgaagcaaat gccggtagag tcttttttct tcgcggtgac cagaccaaga tcttcagcaa
     5941 tcttacgcac ctgcggtttt tccagttcgc cgaccgggaa caggctttgc gcaatctgct
     6001 catggctgag cgtataaagg aagtagctct ggtctttatt gctgtccaga ccacgcagca
     6061 ggcggctctt gccatcgaca tcggcccgac gtacgtaatg accggtagcg atataatcgg
     6121 cacctaaatc ttcggcggca aattcgagga aggctttaaa tttgatctcc ttgttgcaca
     6181 gaatatccgg attcggcgtg cgaccggctt tatattcggc aaggaacagt tcgaagacgt
     6241 tgtcccagta ctcggcagca aagttaacgg tgtgcagttc aatgccgagc ttgtcgcaga
     6301 ccgcctgggc atcagccaga tccgctgccg ctgtgcaata ttcctcaccg tcgtcttctt
     6361 cccagttctt cataaacagg ccttcgacct gatatccctg ttgttgcaac agccaagcag
     6421 aaacggagga atcgacaccg ccggacatgc cgacgattac tttttttgcg gtttcagaca
     6481 ttggatcact cacgacattg aacttcaagg cggcgtattc tatcacgcag ccttttagat
     6541 gacacccttt gtaaaaggcc agttaaaatc gccaatcatc tccagcggat aacgttgccc
     6601 gctttgataa caacgaatac tttccgccac cagcggcgaa cgaagatttg acgcctgtaa
     6661 aatttcttcg gcgctgaccc aacggcagca gtcgatatcg ctgtcatgag gctgggtcgg
     6721 gcatatttgc tcaagctcaa tggcaaagag gaaacgcaaa aacggcgttt tatctggcgc
     6781 aatccactga tgcatacgaa taaagtgttg cggctgcgcg ctgatgccgg tttcttccca
     6841 cagttcacgg gcggcggctt ccactaaggt ttcatcggct tccagatgcc cggcaggttg
     6901 gttccataac gctttaccat taatcgtctc ttcaacgact aaaaatttgc cttctgcgtg
     6961 caccacgcaa gcaacggtaa cgtgcggttt aaacattttg catccttaat ctgtcacttc
     7021 tcgccattcg ccattggcaa gattatccaa agagtaatct cccatcgcat agcgaatcag
     7081 tcgcagcgtg gggaaaccaa catgggcggt catgcggcgc acctggcgat tacgtccttc
     7141 atataaggtg atcttcagcc agctggtggg aatacttttg cgttcacgaa ttggtggatt
     7201 ccgcggccat aaccacgcgg gttcgtcaac cagctccgcg cctgcgggca gggtagggcc
     7261 atcatttaag gttacgccat tgcgcaaggc ttcaagtgcg tcttgtgtgg gaataccttc
     7321 cacctgcaca taatagattt ttccggtgcg tttacccggc tgggttaaac gcgcctgcaa
     7381 cgcgccgtta ttggtcagca ccagcaaccc ttcgctatcg cggtcaaggc gacctgctgc
     7441 ataaacgccc tgaaccggga tgaattcttt taatgttttg cgtccggctt catcggtgaa
     7501 ctgcggaaga acatcgtagg gtttattgaa gaggatcaca cgcgtgggct ggttttcatg
     7561 tttacgcctg gtagaacgtt gcgagctgaa tcgcttaacc tggtgatttc taaaagaagt
     7621 tttttgcatg gtattttcag agattatgaa ttgccgcatt atagcctaat aacgcgcatc
     7681 tttcatgacg gcaaacaata gggtagtatt gacaagtcaa ttacaaatca ttaacaaaaa
     7741 attgctctaa agcatccgta tcgcaggacg caaacgcata tgcaacgtgg tggcagacga
     7801 gcaaaccagt agcgctcgaa ggagaggtga atggaaagta aagtagttgt tccggcacaa
     7861 ggcaagaaga tcaccctgca aaacggcaaa ctcaacgttc ctgaaaatcc gattatccct
     7921 tacattgaag gtgatggaat cggtgtagat gtaaccccag ccatgctgaa agtggtcgac
     7981 gctgcagtcg agaaagccta taaaggcgag cgtaaaatct cctggatgga aatttacacc
     8041 ggtgaaaaat ccacacaggt ttatggtcag gatgtctggc tgcctgctga aacccttgat
     8101 ctgattcgtg aatatcgagt tgccattaaa ggcccgctga ccactccggt tggtggcggt
     8161 attcgttctc tcaacgttgc cctgcgtcag gaactggatc tctacatctg cctgcgtccg
     8221 gtacgttact atcagggcac tccaagcccg gttaaacacc ctgaactgac cgatatggtt
     8281 atcttccgtg aaaactcgga agacatttat gcgggtatcg aatggaaagc tgactctgcc
     8341 gacgccgaga aagtgattaa attcctgcgt gaagagatgg gcgtgaagaa aattcgcttc
     8401 ccggaacatt gcggtatcgg tattaagccg tgttctgaag aaggcaccaa acgtctggtt
     8461 cgtgcagcga tcgaatacgc aattgctaac gatcgtgact ctgtgaccct ggtgcacaaa
     8521 ggcaacatca tgaagttcac cgaaggcgcg tttaaagact ggggctacca gctggcgcgt
     8581 gaagaatttg gcggtgaact gatcgacggc ggcccgtggc tgaaagttaa aaacccgaat
     8641 accggcaaag agatcgtcat aaaagacgtg attgctgatg cattcctgca acagatcctg
     8701 ctgcgtccgg ctgaatatga tgttatcgcc tgtatgaacc tgaacggtga ctacatttct
     8761 gacgctctgg cagcgcaggt tggcggtatc ggtatcgccc ctggagcaaa catcggtgac
     8821 gaatgcgccc tgtttgaagc cacccacggt actgcgccga aatacgccgg tcaggacaaa
     8881 gtaaaccctg gctctattat tctctccgct gagatgatgt tacgccatat gggctggact
     8941 gaagccgcag acctgattgt taaaggtatg gaaggcgcga tcaacgccaa gaccgtaacc
     9001 tatgacttcg agcgtctgat ggaaggcgct aaactgctga aatgttcaga gtttggtgat
     9061 gcgatcatca agaatatgta atcactacat gtgttaaata tttaaacggg cgtataacac
     9121 gcccgttgtt ttatttatgt ggatattatt aatagcatat cgagcatatt tatataaaac
     9181 ctattacttg agcccatatg ggcatatttt tataatacaa ctcttatgca aacatttatt
     9241 tgttatttcg ctttctcctg gaggacactc ttgactgctt ttgagtaaac tccataaatt
     9301 cttgttgaat ggtgcaatgt tataaatagt aataggatat tctttttcct taaggataat
     9361 accaagctta accggtgtaa atatccttcc aggaggaaga aatatagtag attgatacca
     9421 gatgatcatt ttcatatcac cccatatggc tgaaaaagat atgccgcatg taggttgaat
     9481 taccgtgtca attactaccc acttcatttg ttatgtctta tcccacggta tttaatatag
     9541 ttcatttgga tgttcatttc tttattttgc atatgagtat attacccctt caaaaaataa
     9601 attaattaaa acgattgctt atataaaaca aaatttaaat caaggaatct caatggatgt
     9661 taaacaaaat gagatttagt gaaaacaata aattattcac ttcgttttag atttgtttag
     9721 ctataatgtt atacattcaa atgactgaac atcctgtaat taaaacatag cctttatgct
     9781 acttggtgcc aatttgctaa acattatggt tgccttttta tataacgata ataatgaata
     9841 taaacatgac atgagaataa ggtttcaatt tttgagttat ataggaaagg tttaacctgt
     9901 tcctggctaa aatacatata actggatgat gactaaacca aaacacatat gcgttaagta
     9961 ttgaaacggg cgtatggcac acccgttgtt tttataaata tattaaccgt tataaaataa
    10021 cgtataaaaa gtcaagtgat cacatttcaa atatcaattt gatagtatgg gcttggtgat
    10081 tatttatggg tagcaataaa aggacagtat ttatcatcca tagggatagt ctctgtactt
    10141 ttattcccat tatgctaatg ccttactgaa ttatgaagca tttcttaagc atccaacttt
    10201 agctagatta atggtttatt attttctaca tcttcaatat ataaaagcgt attatcaatg
    10261 gcgtagtaac tgcgtttgtt atgattaaca tcagtaaccc aacggaaaac gcccgcgcct
    10321 gctagtgttg aacagtattc ccgaaatgta gattttccgc aaatatgaag caatgcggcc
    10381 tcttttattt tagcaggatt cttggtcgta ctaactttta actggttcct ggttcctctt
    10441 aataacaaaa ccgtgtcatc gtgagtaata attctgatgt tatccgtagc cagataataa
    10501 atgtaatgtg caatacggtg atgttttaat tctgaataaa accaggagaa gttttgctct
    10561 tttctcactt gctcaaacat cttttgaaaa acaacgacct gatccatcag gataataacc
    10621 tcttgttagt tgtgagactg cgtagtgtgc acgatcggtt ttaccacttc aatctggtct
    10681 gtcctttggc tgtgatatgt acagagtgtg atagagggat tacctgaatt ctcccggtga
    10741 gcattttgca acggaccagc tccggtacaa atgctgttgt gggttcagat tataacattc
    10801 tgtctaaggg gcgggataaa ggtgaaatta gggggcatga aagatgactt tataaccttg
    10861 ctcaccccaa tgttgtaaaa gttcgttttg ccttctcgtt ggtgccatgc ctgtccagac
    10921 aatcaatgtt tgcgtcggga acagttccgg gcgcggcgat tcaatgggat cagcaagaac
    10981 agaaatgtgc catcctgaca atgataaacg ccatgcttcc agccacaatc gtgctctctc
    11041 ttcaacattc cacgccatca gaatggcttc tttaccgggc ttacggcgcg tttcgaaaag
    11101 cgaggttgct gcgtattcaa ttaatgcgcc gtcaaacata ctgctcataa tgcgggaggt
    11161 gttgtgatca agcacgagac gctggcgaac aggaaggtaa acatgattaa tcaattgatc
    11221 tactgggtac tctcgaccca gtgaaataat tctcgcgcgt agtttggcag gattagccat
    11281 acgaagaatt gacatcatct cttcttgcag gcggctccag tcatcttccg tatcctggct
    11341 ggtggtttcc agtaatgctt taactttgcc tacagggacg ccattactga tccaacgctt
    11401 gatctcttcg atgcgttgta tgtcttcttc atcaaagagt cggtgtccgc cttcactgcg
    11461 ctgtggtttt aacaaaccgt agtggcgttg ccaggcccgg agagtgacag gattaatccc
    11521 gcaacgttca gcaacatcac caatgctgta ataagccaca attcctcctt gcggtcacaa
    11581 atctccgtcg ccggtacacg acccaataat actttgtaca atatacgcta aaattgtaca
    11641 aagtataaat aagatttcag ctaaattgga tgaaacatta tttttaatgt agattaaatt
    11701 taaacataac gtattcattt tcacgatgat ttactgaaat catgtgaaag aatgtgctga
    11761 aaattatttt ttctctggcc acgctatgga agggatacca ttcaatttag ctttagcaaa
    11821 cagatctccc tgaaacatct caattcctgc ggattcaagc cacatccact cttctggtgt
    11881 tgccacgccc atagcactga cttgaatttc aagtgatgta cagcatttta tgatcgcttg
    11941 aataattgcc tgccgtggcc cgcttttatg aacattggta atcaattcct gactgatttt
    12001 aattctgtca ggctggaagc gtgacaggag taacaaaccg gcaaaacctg cgccaaaatg
    12061 atcaattgct acactgatac cggcagcctt aagcgattta atcgcttctg caaactcatc
    12121 aaaccgagat ataacttcac tttcagtaaa ttcaacgatg atttgttcag gcaccagagc
    12181 attggccttt atttcatcaa gtaaaaaaga gactgcgtca ggttcgttaa ccagggtcat
    12241 aggtaataga ttgattgaaa tcattttatc accgagctca agtgcgtgtg tcatcgcgaa
    12301 tgcaagcgcc ttacttttga gatccgctgt gtagatttcc ccgtctttac gctgcccaac
    12361 cgctatggct gatgggctat cttcattttt ttgcacaatg gcttcaaaag cgattatccg
    12421 ccgcgataag ggatcgacaa taggatgaaa ggcaaagtgg tcgtttatag ttggtgaaag
    12481 ggtacaggaa tcaagttctt tatcagatcc gtcagcgata aaaagccagg agtcttcggc
    12541 agggatctcg aaataggttg attgttcggt tgcaaggaca aaagtacgaa aaaattgtag
    12601 cgctctgtca tcataagtta gctgaaattt tgatgtgcct ttgtcgaata cggcctgtaa
    12661 aacgtcatct cgctcgtgca ggcgcaaatc aaataattcc attcccgctt tgccaaaacg
    12721 gcgagcaggc gcgtaatcgc acagcagctc aacaatatta tagtgccgtg gatcttggca
    12781 tatagcccga tatatcattt taacctgttc ttccggacct tccagaagct ggaaaaaatg
    12841 agaaccatta aacagtaaga tccctgttac gtcagactgc atgttcctgc gatttgctat
    12901 cgaaaccatt tcttcgattt ttttgacagg ttcgtcgtca cgtatatggc tacgataaat
    12961 aagggtggta agcattaaca atccagggta atgggtgagg cgagagtaag acggtaacag
    13021 acatatcttc ttgtgtcttt cttttaatac caaaacataa ccgtttcttt acattgataa
    13081 aaaatggaaa aagttgaaca cgagttggcg aaaaatcttg tatagattgt cagttaaatg
    13141 atgcaatatg ttttatcata acacattgtt ttatatacat tagtactaat tgcaaaaaat
    13201 taatttatca ttctgtacac atatttcgta caagtttgct attgttacct cacttcacat
    13261 tgattaacat ttttaacaga ggcgtagcat gcatcaaaat tcagtgactt cagattctgc
    13321 gggagcaatc actcgttact ttgcaaaagc taacttgcct actcagcagg aaactctcgg
    13381 ggaaattgtg actgagattt tgaaagatgg acgtaatctg agtcgaaagt cgcattgtgc
    13441 caaacttctt tgccgactgg aacaagcgac aggggaagag gaacagaaac attataatgc
    13501 actaattggg ttgctttttg aataatgcaa cagcctgatt atttaacagg ttagctatga
    13561 agtcgttatg aagacatctg ataatgaacg tataaaatat gaaattactg gccaggcggt
    13621 gctccagata ctgcgcatga agataaattt ttcattgcag actcttatta agcaattact
    13681 cgtaatgaaa tcggctgagg aagatgcttt ccgacgcgat ttaattgaca gcataattcg
    13741 tgattttagc aatagtgatt caggagggcc aaaccgaaga acagcgacag ctgacaataa
    13801 aagtatgttc aatggtaaga aaataaacag aatacattaa aatttcataa gtaagatgag
    13861 aggttaccat gcttgaagat actacaattc ataatgcaat aactgataaa gcgttagcaa
    13921 gttactttcg cagttcgggt aatttgttag aagaagagtc agcagtgtta gggcaggctg
    13981 tcaccaattt aatgctttca ggcgataatg ttaataataa aaatattatc ttaagtctga
    14041 tacactccct ggaaacaaca agtgatattc tcaaagctga tgtgattaga aaaacactgg
    14101 aaatcgtgtt gcgatacaca gctgatgata tgtaacttct aattattaag tataagttta
    14161 tagaaaactc attcatctta tttttgtctg tcgctttaga ctatacacag cataatttta
    14221 ttgggttaat atttctacga ggctgacatg aataattcaa tcccagagag atttattttt
    14281 caatgtgctt tgtttaaaaa tctcgagagg gaggtgttca tgacgcatgg gtatgttgat
    14341 agtcatatta ttgatcaggc tttacgcttg cgtttaaaag atgaaaccag tgtgattctt
    14401 tctgatctct atctgcaaat attgcagtac attgaaatgc ataagactac actaacggat
    14461 atcattatta atgacaggga atccgtgctc tcttagaact tgagtaaggt tttagaataa
    14521 acatcagctg tatcaccatg ctgatgcaaa gtgagcattt caggcgttat gctttcttat
    14581 tatgtccgca
//
LOCUS       DAJVQH010000104        13983 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJVQH010000104 DAJVQH010000000
VERSION     DAJVQH010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.104"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..161
                     /locus_tag="OQM87_003102"
     CDS             <1..161
                     /locus_tag="OQM87_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1509157.1"
                     /translation="FAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKRYR
                     KKLTEFNQ"
     gene            266..1618
                     /gene="celF"
                     /locus_tag="OQM87_003103"
                     /gene_synonym="chbF"
     CDS             266..1618
                     /gene="celF"
                     /locus_tag="OQM87_003103"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS1509158.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1631..2389
                     /gene="chbG"
                     /locus_tag="OQM87_003104"
     CDS             1631..2389
                     /gene="chbG"
                     /locus_tag="OQM87_003104"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCS1509159.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2436..4697)
                     /gene="katE"
                     /locus_tag="OQM87_003105"
     CDS             complement(2436..4697)
                     /gene="katE"
                     /locus_tag="OQM87_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCS1509160.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIREHVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4880..5143
                     /gene="cedA"
                     /locus_tag="OQM87_003106"
     CDS             4880..5143
                     /gene="cedA"
                     /locus_tag="OQM87_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCS1509161.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5420..6236
                     /locus_tag="OQM87_003107"
                     /pseudo
     CDS             5420..6236
                     /locus_tag="OQM87_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6240..7630)
                     /gene="tcyP"
                     /locus_tag="OQM87_003108"
                     /pseudo
     CDS             complement(6240..7630)
                     /gene="tcyP"
                     /locus_tag="OQM87_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(7763..8353)
                     /locus_tag="OQM87_003109"
     CDS             complement(7763..8353)
                     /locus_tag="OQM87_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1509162.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(8516..9183)
                     /gene="hxpB"
                     /locus_tag="OQM87_003110"
                     /pseudo
     CDS             complement(8516..9183)
                     /gene="hxpB"
                     /locus_tag="OQM87_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            9330..9866
                     /locus_tag="OQM87_003111"
     CDS             9330..9866
                     /locus_tag="OQM87_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCS1509163.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(9907..10767)
                     /locus_tag="OQM87_003112"
     CDS             complement(9907..10767)
                     /locus_tag="OQM87_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCS1509164.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPSFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(10873..11163)
                     /gene="ghoS"
                     /locus_tag="OQM87_003113"
     CDS             complement(10873..11163)
                     /gene="ghoS"
                     /locus_tag="OQM87_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1509165.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(11264..12193)
                     /gene="pfkB"
                     /locus_tag="OQM87_003114"
     CDS             complement(11264..12193)
                     /gene="pfkB"
                     /locus_tag="OQM87_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCS1509166.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            12479..13237
                     /locus_tag="OQM87_003115"
     CDS             12479..13237
                     /locus_tag="OQM87_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCS1509167.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(13290..13397)
                     /locus_tag="OQM87_003116"
     CDS             complement(13290..13397)
                     /locus_tag="OQM87_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509168.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(13569..>13983)
                     /locus_tag="OQM87_003117"
     CDS             complement(13569..>13983)
                     /locus_tag="OQM87_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509169.1"
                     /translation="EPGLYAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLLKGA
                     TAQGPPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSAVHI
                     AIHHKHYKTVETYYAAINVISQSLSFSADEIKTYL"
BASE COUNT         3607 a         3212 c         3455 g         3709 t
ORIGIN      
        1 attttgccaa aaaacaactg gaaatgacca actattccgt gacggatatt gcgtttgagg
       61 ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg tcctttacgc
      121 ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata cctgacaatt
      181 cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg ccagatatca
      241 gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca ctattggtgg
      301 cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc acgaattgcc
      361 ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg atattatttt
      421 cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt ataaaacgct
      481 ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc gcgttggcca
      541 attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc ttggtcagga
      601 aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga tttttgacat
      661 cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca ctaacccggc
      721 gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta tcggcgtgtg
      781 taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga aagacagcga
      841 tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaaga atgtgctggt
      901 aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc agttaaaagc
      961 atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc gttctctgaa
     1021 tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc tggctattga
     1081 aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag tcgagaaaca
     1141 actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac tggaacagcg
     1201 cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct acaacgacaa
     1261 gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata ttccggcaga
     1321 ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac atccgcgcat
     1381 tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct tcgagattgc
     1441 tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc taaaccttag
     1501 tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga ttctggcgca
     1561 cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag cacattaacc
     1621 gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct taagcaaagg
     1681 tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga cgacggcgct
     1741 ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac cgagtctggc
     1801 catagggatg cactttgtcc ttactatggg caagccactg acagctatgc cggggttaac
     1861 ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg ctttaccgct
     1921 ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc tatttggacg
     1981 caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga ttttcccgat
     2041 tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc agccgctatc
     2101 taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta gcgcgtttta
     2161 tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa gccatcgggg
     2221 tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta tccgtcagag
     2281 tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt cgttgaaata
     2341 tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag taaggtggta
     2401 aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt gtcaatctta
     2461 ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc cataaaacta
     2521 ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac cttgattgtt
     2581 gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt gtaggcttcc
     2641 atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc gcaagggaca
     2701 atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat aggcaacacc
     2761 gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc atgaacgcct
     2821 ttggccttca gggccttgag aatggcaaga aggtctgccg atctcacttc atcattaagt
     2881 aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa acttaaggat
     2941 ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg gtcgtcagtc
     3001 agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat atgcgccagc
     3061 tggtcaacaa cgtgctcacg aatatacgga cgaacgactt tgcttaactc aaaactgaaa
     3121 ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag acgcggatgg
     3181 gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc cacgcgctct
     3241 tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg ccagttatcg
     3301 ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg atgcatgccg
     3361 tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc atggaaattc
     3421 ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc ctgcaacagg
     3481 ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc tgcttgttcg
     3541 ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac acgctgaacg
     3601 ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc gaacttgaat
     3661 tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc gcctgcttca
     3721 atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag tttttgtgct
     3781 tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg tacaaacgtt
     3841 gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc catggtgcgg
     3901 taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag agtttcaggt
     3961 tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg aattgcccag
     4021 tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc ctggataaag
     4081 aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata gaacttggtg
     4141 gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg aacggtagag
     4201 aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc tttggtgata
     4261 tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgatccgcg tgcatgaaca
     4321 atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag aataaaatct
     4381 tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc ggcgatgcgc
     4441 acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc ttccagagaa
     4501 ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt aggttgtgca
     4561 cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc cagtgagtcc
     4621 atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg tgggttcttt
     4681 tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg ccgctgtttt
     4741 ggtcaaaaaa ctctctaact atagaccacc agccagattc cgcttcggag actattctgg
     4801 aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg gctaaactac
     4861 agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac cgctccgcca
     4921 gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc cagaacatgc
     4981 gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg ttgcgtttgt
     5041 actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat ctgcccaacg
     5101 ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac atacctcctg
     5161 gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt cattcaaacc
     5221 acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagtgat cttttttctt
     5281 taattgttta tttattttaa aatggttaag ttttgctaat catgatgagt tatttttgat
     5341 agttcatcct gtggcgtcgc tcttaaactt atattgtaaa tggcttagat ttaaataaga
     5401 taataatttt gttaacaata tgaggcggga gatgttttct acaaacttaa tacagagtaa
     5461 ttatggtgat ttaaatatta aaagcctggc tttcgactct tttaaagaaa ggcttgagtc
     5521 taccatggcg gcactcactt tctttatatc aacgggtcaa tgtgactgcg atgaaatagc
     5581 agagtcgaac ttcaatcgta tgattgccta tatgtctaac attaactatg atgcctcaaa
     5641 gctaggagcg ccagcactta gttttgatac gtatctgcag gacaatgtta aatatagagt
     5701 gataattaat aatctttatg gcagtgagtt tcggataaga gatattaatg aggattttgt
     5761 tgggatggat tttacctgtg aattccgacc cgaaaaaatg acgaatttga tcaatataaa
     5821 aaataggttg gttattcatt atttaaggac tatatactat gaaaaaaatt atatacatcc
     5881 tgccgggagc atttttgctg cgattcaaaa aaaatgagag tctcctaaag tttccttcga
     5941 taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg ggaatgggaa
     6001 gtggtgtgct tgaagatatt atgagtattc cagatagttc acttagaaaa cgtttcgggt
     6061 atgaggtttt aagcttctca ctacaggcac attcattgag ccaggaatgt attgataagt
     6121 tggacatttt tttcgctgac gatctgttta aatatgagtc agtatgcatc gccgcgatgg
     6181 aacatttaaa aagtaaagca acggccccta tacaaaacgg accgttgcca gcataagaat
     6241 taacggtgtg ccagttcggc gtcgtcttcg ctatccagaa tggctttatc ggtttgcttc
     6301 agccactggc tggtcagcgt gccagctgtc atcgagccac taacgtttag cgccgtacgg
     6361 cccatgtcga taagcggttc aacggagatt aacagcgcca ccagggttac tggcaggccc
     6421 atcgcaggca gcacaatcag tgcggcgaaa gttgcaccac caccgacacc ggcaacgcct
     6481 gcggaactaa cggtaacaat accgaccagc gtcgcaatcc acatcgggtc cagcgggtta
     6541 atgccaaccg taggcgcaac catcaccgcc agcattgccg gatacaaacc ggcgcagccg
     6601 ttctgaccaa tggttgcacc gaaagaggcg gcgaaactgg cgatggattc aggaacgccc
     6661 agacgacgcg tttgtgcttc cacattcagt gggatagacg cagcactgga acggctggta
     6721 aaggcaaacg tcagcacagg ccatacctta cggaagtact tcagcggact cacgccatta
     6781 atgcccagca gaatgccatg cacagcaaac ataatcagca gaccgaggta ggacgcgaca
     6841 acgaaacttc ccagtttgat gatgtcttgc aagttagaac ctgcaaccac tttggtcatt
     6901 agagccagaa cgccgtaagg ggtcaactgc atgacaaggc gaaccagttt catcacccag
     6961 ctttgtaggg tatcgatagc ggttaagacg cgttcacctt tcggcgcatc atccttcagc
     7021 agtttcaatg cagctacgcc gaggaatgcg gcaaaaatta ccacgctgat aattgacgtc
     7081 ggattggctc cggtaagatc ggcaaacggg tttttcggga taaaggacaa gaccagctgc
     7141 ggaacgctca gatcagagac tttaccaaca tagttacttt caatggcgtt cagacgtgca
     7201 gtttctgcac caccctgaac cagaccttca gccgtcaaac caaacaggtg gtgaccagca
     7261 caccgaccag cgccgcaatc agcgtggtaa acaaaagcgt accgatggtc agaaaactga
     7321 ttttgcctaa ctgagatgcg ttatgcagac gggcaaccgc gctcagaata gaggcgaaga
     7381 ctaacggcat aacgatcatt tgcagcagtt gaacattgcc gttaccaacg atgttaaacc
     7441 actgtacaga atctttaagt acctggctgt cagaaccata aatggtatgc agggcaaggc
     7501 caaaaaccac acccatcacc agacccacca gcactttttt cgccagactc cactgtttat
     7561 ggcgggtctg agccagcgca aacagcagta caacgaacac cacgatgttc gcaattaatg
     7621 gaaagttcat ccccgttctc ctgatcttat tatttctatc actgcgtagc agtcatagtg
     7681 ttggcgcaag attaacagaa ctgtgagctg gctcttatat tcaaatggaa ttgcttattc
     7741 cagaaaggtg atttttgcct ttttagtatt caacctgatg cttaataagc cgatgaaact
     7801 ggatctgcaa ggtattgatc atttgcgatg accaacgaac agcgctgttc tcgggtaatg
     7861 aatggggcat ccataccgac cagacaaata atgccatgca gataaaacgt tccagttggt
     7921 tgcccttttg gggaaccagg ataggcaagc ggaaacgcca gcggcatggc cagagcaggg
     7981 gaacaccggc gggtgtcagc atatcggcaa gtatgtggct caaataaccc agcaccattc
     8041 cttgtagcgc atcagccgga atgaaccagc cttccggaac cttaaggtaa aaggttgcca
     8101 gcagcgcaaa taccgccagc agactgtggg taaaaccacg atgcccaaaa gcgcgggcga
     8161 tcggttttga tatccatttt aatcgctgcc caagaaacga ctttgggtga tcgatgtccg
     8221 gtaacaaaca cgtcaggatt gcggaaggga caatatgcca ccagtcaccc tgtgccagca
     8281 cgggcgtcag ctcggcattt ttggcaaata ccgcacaagc aatagaaaag agaaggtgac
     8341 cttccgccgt catgataaaa ctcactaaac tgtcgatacg tacagtatag gatttttata
     8401 cagtgcacgg aagtggtgac ggtgtaactc tttctcttaa attgacgatt aatccgtcag
     8461 ttgaatgcaa attcagataa agaaccaggg gcagcgtgat cactgcccct ggtcattaac
     8521 cgagaaggtc ttttgcggtg agttctgtca gcgatgagag tttgacgttt gctaatacaa
     8581 aacgtggatc attttgcgct tctggcgcag gaacgacgat ggaacgcatg caggctgctt
     8641 tagaagcgat catgccattt accgagtctt ccagcgccac gcaggtcagt gggtcaacgc
     8701 ccagttttgc tgcgcagtcg agatatactt gcggatgcgg cttgctgtaa ggcagttttt
     8761 cagccgaggc gagggcatcg aaactgtcgc gtaagtcaaa catggtcaac actttttcca
     8821 gcatatgtag tggtgacgcg gaggccagtc ccaccaataa accttgttct ttgcataacg
     8881 caacagcttc gcgcacgcct ggtaataatg gacgtgtctc ttcaaccagt gaaatggcac
     8941 gggcaataac ccgttctact acttcctgac ggcttggccc attccacggt tgccgggcgt
     9001 acaaagatcg accaccatat cgatgcgtaa acctaaggtg tccggcagct cgttacgacg
     9061 ggagatatct acccccaggc ttgccatcac atccagttcg gctcgatccc ataaaggttc
     9121 tgagtcgata agtaatccat ccatatcaaa aattgcagca agaatctgac gcggggttga
     9181 cattacaacc tctcctttag agcattatgt gtaaggtatt aagccgtgct tatgattaag
     9241 cgcctatcat atcgccttta aagaatgggc gaaaacagat atctacaggt aaactaagtg
     9301 aaacgacgcg tcttaatcaa ggagaactca tgacgtatca acaagctgga cgcattgctg
     9361 ttttgaaaag gattttgggt tgggtgattt ttatacctgc attgatctct acattgattt
     9421 ctttgctgaa atttatgaat acccggcagg aaaaccagga aggcattaat gcggtcatgc
     9481 tcgactttgc tcatgtcatg atcgatatga tgcaggcgaa tacgcctttt ttgaatctgt
     9541 tctggtataa ctccccgacc cctaatttta atggtggtgt gaacgttatg ttctgggtga
     9601 ttttcatcct gatttttgtc ggactggcgt tgcaggattc cggtgcccgg atgagtcgcc
     9661 aggcgcgctt cttgcgggaa ggtgttgaag atcaactcat tctggaaaaa gccaaagggg
     9721 aagaggggct aacgcgtgaa caaattgagt cccgtattgt tgttccgcac catactattt
     9781 tcctgcagtt tttctcacta tatatcctgc cggttatttg tattgctgca ggttatgtgt
     9841 tcttttctct gcttgggttt atttaatcaa aaggccgcac ggagcggcca ttatcctcaa
     9901 cccatatcat gctgctaata atctatccaa tgactgctga gcaataacca aatgctgacc
     9961 gccaaataat cttgcacgat ttaacagcgt gtagagttgg taaaccggtt gacgttcaag
    10021 gaaatcggca ggtagcgggg atactgactg atagccgtca tagatttgtg gcggctgttc
    10081 agtatgcagc ggtaacatcg ccaggtcgca ctctcggtca ccccagtagc aggcagggtc
    10141 gaaaatgtac gggccatccg gacccagcgc acagttgccg gaccataaat cgccgtgcaa
    10201 cagagaaggc tgcggttgat gcgaggccag acgctgctga atatgctcga cgatggcgtc
    10261 gatattgccg aaagcgatcc ctttctctgc tgccagttcc aactgccagc caatccgttg
    10321 ttcagcaaaa aacgttgacc agcgacgttg ccaggtgttg ggctgtggag ttgtggagag
    10381 cgagttatca aaatcgaggc caaattgtgg ttggtcactc cattgatgta aacgcgcaat
    10441 ttgctgacca agaataaatg cgctatgcgc atccagcgga cgaggtggga gataatccat
    10501 caccagaaaa ctgtagtcac ggtcagcgcc tactgcccaa accttaggca cggtgacggt
    10561 tttactacgc gacagtaact ccagttggtc ggcttcggcg gtaaaactgg gaagcagttc
    10621 cctttcatca catttgacga aaaagtcatg tcctgcatag cgcaaatgcc atgcggcatg
    10681 gacttctccg ccaggcagtt cattacgcag ttcgatttcg ccttcaccta actgctcgct
    10741 caaaagacga ctgattgcct gccacatgat gtctctccca tgttgtctgc cagatcataa
    10801 ggttagcgca agaatgcggt gtaaaaacac gagctaacgc acaccacaga ctgattattg
    10861 cccaaaaacc atttattttc tgttgctatt ccattcctcc caggtcgtga tggtgatttc
    10921 aggatctttg ccggttgcac tttgggtaag ccccgaaacc agttctctaa tttcttcttc
    10981 actttgggta ctgataagtc caaaagtgtt agtccccagc tcatggatat ttccctcatc
    11041 atccgtcatg gtgagcaaaa aaccgtcgcg agtcaggtaa ttattcaact cgttaatttc
    11101 agtcaatgta tcctcatgca acatgacggt tatgacataa cggacaagat cgccactggc
    11161 catagttcac ccctttgtta tcacatgact tttgagcata gtcggagaaa cgcgttgccg
    11221 acaggttggt ggtgattccc ccaatgctgg gggaatgttt ttgttagcgg gaaaggtaag
    11281 cgtaaatttt ttgcgtatcg tcatgggagc acagacgtgt tccctgattg agtgtggctg
    11341 cactccccgc agctacgcca aaacggacca tctcttcaag agaggcattt tctgccagtt
    11401 tcagtgtcat cgcgccgacc atgctgtcac cagcgccaac ggtactctgg cttttcaccg
    11461 gtggtggcac cacctgaatg cagttttcac tatcaacacc cagcgctcct tgtggaccaa
    11521 gggaaacgac aacccgtttg gccttgccgc tattaacgat ttcctgcgcg gctttgcgga
    11581 catcgtctgg ctgggtgagt tcgcgactca ccagcgcact gagttctttt tggttaggct
    11641 taaccaactc gatgttacca atcgccagtg ctgcacttaa cgcttcgcca gaactgtcga
    11701 tgatgcagcg gatcccttgg ttttgcgcag cggaaatcag ttgggttaat ttttccagct
    11761 tcacacctgg cggcaggctt ccgcttatga ccaggatggc cccggattca atttccagaa
    11821 cttgctcttc aagctggcga aactcatctt catttaatgc cgcgcctggc ataacaaaac
    11881 gatactgttc accgcttgct tccacatgta cgtgtaaatt ctgccgggtc cagtctttgg
    11941 cttctacagt agcgacgggg acattttcat ccgccaacag tgaaaccagg tgttcgccgg
    12001 tcgcgccacc cgccgggaag atcgctgtgg cactgcctcc aagatgggca atggcgcggg
    12061 cgacgttgat gccgccgccc ccgggttcga acaccggtgc ggtacagcgc agttttcctt
    12121 cgggataaat ttgcggggta attgttgcgc tatcgagaga gggcgcaagt gtcaacgtat
    12181 agatacgtac catcatttcc tcctataggc tgatttcagt ctggcaccga atcagcggaa
    12241 agtgaagaaa ttaacaatat gatttattag agctttattt aaaattttgc agataaatat
    12301 atataaataa aaatctgttt tcgccacatg cttattcaaa aaatgaaata agaatacatt
    12361 gctaagttat tcgcgccttt ttataattcg ttacctttcc agggacgcta atctttgatc
    12421 cttaagaaag tttccgggac cgtgatggtc tcttttttgt tgtacggact cttgataaat
    12481 gaagcttttg aagacagttc ctgccatcgt tatgttggcg gggggcatgt ttgcgtcact
    12541 gaatgcagct gccgatgatt ccgtttttac tgtcatggac gatcctgcca gcgctaaaaa
    12601 acctttcgaa ggtaatctga acgcaggata tcttgcacaa tcaggcaata cgaaaagctc
    12661 ctcacttacg gctgacacca ccatgacctg gtatggacaa accactgcct ggtcgctgtg
    12721 ggggaacgcc agtaatactt cttctaatga tgagcgttct tcagaaaaat atgcggcggg
    12781 cgggcgtagc cgctttaatt taactgatta tgactattta tttggtcagg caagctggct
    12841 gacagaccgt tataacggct atcgagagcg cgatgtgttg accgcgggtt atggtcgtca
    12901 gtttttgaat ggcccggtac acagtttccg ctttgaattc ggtccaggtg tgcgttacga
    12961 caaatatacc gacaatgcca gtgaaaccca gccgctgggc tatgcctccg gggcttatgc
    13021 atggcagttg actgacaacg ctaaatttac gcagggtgtt tcggtctttg gcgcggaaga
    13081 cacaacgctg aactccgaaa gcgcattaaa tgttgctatt aatgaacatt ttggactgaa
    13141 ggtggcttac aacgtcacat ggaactctga acctccagag tctgcaccgg aacataccga
    13201 ccgtcggaca accttatcgc tgggctacag catgtaaaat tatctgccgg accgtgttat
    13261 gcggtctggc tgtcttcgtt tgtcactgct cagccccaaa tcattgctgc ccaacgtagc
    13321 aacaacatag cgccacagat tgccagtagc accaccacca tcttccagtg ttttttatca
    13381 aagcgtttag tcggcatacc agtgtcctgc atatctcttg tgcgtgataa cggagtgtaa
    13441 caaaagggac aggcatcaag ggattttttg ttattacagc cgtcgggtgg cgttgtgcac
    13501 atggtcactg tttctgcgtt ttatgaaaaa aataaaatta tgtatcgata tatatcgtaa
    13561 atagctggtt ataaatacgt ctttatttca tcagcactaa aactcaggct ttggctgatg
    13621 acatttatag cagcataata tgtttctaca gttttataat gcttatgatg aatggctata
    13681 tgaacagctg acatgttgtc atgattttta gcggccaata gtgtaaataa ctgatgttga
    13741 ctaaaagaat gtttttttgc aaaagcaccc agcgtcgata tataagataa cacgacgtct
    13801 tcattaccct tgctcatggc gatgtataat gcagggggtc cctgtgctgt agctcctttt
    13861 aataaatcca tgatgttagc tttgctcaac ttgtatttaa aggcgatgcc attaatttta
    13921 gagaggaacc gtgtgacaca caaagggtga ttattttcca ttgcggcgta taatcctggc
    13981 tca
//
LOCUS       DAJVQH010000105        13955 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJVQH010000105 DAJVQH010000000
VERSION     DAJVQH010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.105"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..494)
                     /locus_tag="OQM87_003118"
     CDS             complement(<1..494)
                     /locus_tag="OQM87_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1509170.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(525..1217)
                     /gene="fimC"
                     /locus_tag="OQM87_003119"
     CDS             complement(525..1217)
                     /gene="fimC"
                     /locus_tag="OQM87_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCS1509171.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1418..1960)
                     /gene="fimA"
                     /locus_tag="OQM87_003120"
     CDS             complement(1418..1960)
                     /gene="fimA"
                     /locus_tag="OQM87_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCS1509172.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2441..3307
                     /gene="folD"
                     /locus_tag="OQM87_003121"
     CDS             2441..3307
                     /gene="folD"
                     /locus_tag="OQM87_003121"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCS1509173.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3309..3521
                     /gene="ybcJ"
                     /locus_tag="OQM87_003122"
     CDS             3309..3521
                     /gene="ybcJ"
                     /locus_tag="OQM87_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCS1509174.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3629..4150
                     /locus_tag="OQM87_003123"
     CDS             3629..4150
                     /locus_tag="OQM87_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1509175.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4186..5571)
                     /gene="cysS"
                     /locus_tag="OQM87_003124"
     CDS             complement(4186..5571)
                     /gene="cysS"
                     /locus_tag="OQM87_003124"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCS1509176.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5745..6239
                     /gene="ppiB"
                     /locus_tag="OQM87_003125"
     CDS             5745..6239
                     /gene="ppiB"
                     /locus_tag="OQM87_003125"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCS1509177.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6242..6964
                     /gene="lpxH"
                     /locus_tag="OQM87_003126"
     CDS             6242..6964
                     /gene="lpxH"
                     /locus_tag="OQM87_003126"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCS1509178.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7082..7591
                     /gene="purE"
                     /locus_tag="OQM87_003127"
     CDS             7082..7591
                     /gene="purE"
                     /locus_tag="OQM87_003127"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCS1509179.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7588..8655
                     /gene="purK"
                     /locus_tag="OQM87_003128"
     CDS             7588..8655
                     /gene="purK"
                     /locus_tag="OQM87_003128"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCS1509180.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8792..9685)
                     /locus_tag="OQM87_003129"
     CDS             complement(8792..9685)
                     /locus_tag="OQM87_003129"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS1509181.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRWVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9682..10329)
                     /locus_tag="OQM87_003130"
                     /pseudo
     CDS             complement(9682..10329)
                     /locus_tag="OQM87_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10332..11564
                     /gene="allC"
                     /locus_tag="OQM87_003131"
     CDS             10332..11564
                     /gene="allC"
                     /locus_tag="OQM87_003131"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCS1509182.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11575..12360
                     /gene="allE"
                     /locus_tag="OQM87_003132"
     CDS             11575..12360
                     /gene="allE"
                     /locus_tag="OQM87_003132"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCS1509183.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(12588..13733)
                     /gene="glxK"
                     /locus_tag="OQM87_003133"
     CDS             complement(12588..13733)
                     /gene="glxK"
                     /locus_tag="OQM87_003133"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HCS1509184.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDALFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(13755..>13955)
                     /locus_tag="OQM87_003134"
     CDS             complement(13755..>13955)
                     /locus_tag="OQM87_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCS1509185.1"
                     /translation="QQITFTERNIYRLALPLFVGIFLMALPPVYLQDLPLTLRPLLSN
                     GLLVGILLAVLMDNLIPWERIE"
BASE COUNT         3386 a         3680 c         3497 g         3392 t
ORIGIN      
        1 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
       61 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
      121 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
      181 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
      241 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg ataaacgccc
      301 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct
      361 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
      421 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
      481 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
      541 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
      601 actcctgagg gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac
      661 ttttggctgc ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca
      721 ttgatatagt taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct
      781 tgtaattgaa ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca
      841 ttcctgccgt tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac
      901 agagactcac gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
      961 cttttgggtt cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg
     1021 ctgctgtttt caatccacga attgaccaga aagagctcat tggtatggct atttttaatc
     1081 cataccgcag tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc
     1141 cctccggcag catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac
     1201 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa agattgcttt
     1261 ggcttcttac aacacatcat tcccttttca tttaataaag cggcatgata caaagtgaac
     1321 gtggtttcga tttacattgc caccataccg cttcagacaa ccggtacatt actttgttat
     1381 aagacgtgat atataattca aaacaacgtg gttttgatta ttcgtatctc ataataaaag
     1441 tcgcgtcagc atttgcttgc cctgctgatg cacttgtacc ggtgccttta taacgtgcag
     1501 aaaaatgaag aacgttggtc ccggggatca agttctggtt ggttgagaag ctattcccat
     1561 caggtttgag aattgcggat gtattatcaa gtatttcaat accgacaccc gttgctgttg
     1621 ttgtatttgt actggatgca atggccaata aattatcatt gattgcatca gcctgaccag
     1681 aaaatgcaac ggcagcatta gcggcaacaa caggatcgca gtcgttcaac tgaatggtga
     1741 atggaattaa tgcagaggta ttaccaacag cattgaaaat atcggtacga tattgaccga
     1801 gtgtgacaac ctggtctgcc gagtcagtat tcactgaaca ggcagcattc accagttcgc
     1861 cttcaaagtg aattgtaccg ccatctacaa cggcagcata actacccgta gcggcgaata
     1921 gtgcggcagc cagcgcagac gaaataaatc ttaatttcat atatattcct tcaatctcat
     1981 ttatcgactc cacatccgta tataaccgat tactttattt aagacactga tagtagtaaa
     2041 attccttttt atcctctaag aatgtcttaa ttgaaaatat gcactctatt ctaaaaaata
     2101 gagagccccg ttagatgaat acttccgcgc aaaatatatt caacacaaat atagacctga
     2161 agcggtaaat taccaggctg aaaattcttt ttatattgtc aggtatttct taaattatct
     2221 taatccttaa acaaggaaat aaatcagttc cagatttaca acgccatcat gtacgaaaaa
     2281 tgaagctttc aatctcagcg acctcagcga cggtgcgcct aaccttcgca agaggtcgct
     2341 tcacgcgata aatctgaaac gaaacctgac agcgcgcccc gcttctgaca aaatagacgc
     2401 atccccttcg acctacgtaa cagatggaat cctctctctg atggcagcaa agattattga
     2461 cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct caaaaagttc aggctcgtat
     2521 tgcagccgga ctgcgggcac caggactggc cgttgtgctg gtgggtagta accctgcatc
     2581 gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa gtcgggttcg tctcccgctc
     2641 ttatgacctc ccggaaacca ccagcgaagc ggagctgctg gagcttatcg atgcgctgaa
     2701 tgccgacaac accatcgatg gcattctggt tcaactgccg ttaccggcgg gtattgataa
     2761 cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg gacggtttcc atccttacaa
     2821 cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc tgcaccccgc gcggtatcgt
     2881 cacgctgctt gagcgttaca acattgatac cttcggcctc aacgccgtgg tgattggcgc
     2941 atcgaatatc gtcggtcgcc caatgagcat ggaactgttg ctcgcaggtt gcaccaccac
     3001 cgtgactcac cgcttcacta aaaatctgcg tcatcacgta gaaaatgccg atctgttgat
     3061 cgttgccgtt ggcaagccag gctttattcc cggtgactgg atcaaagaag gcgcaattgt
     3121 gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt gtgggcgacg tcgtgtttga
     3181 agacgcggct aaacgcgcct catacattac gcctgttccc ggcggcgttg gcccgatgac
     3241 ggttgccacg ctgattgaaa acacgctaca ggcgtgcgtt gaatatcatg atccacaggg
     3301 tgagtaacat ggcgacattt tctttaggta aacatccgca cgttgagctg tgcgacttgc
     3361 tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa aatcgcgatt gccgaaggcc
     3421 aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg caaaatcgtc gccggtcaga
     3481 cagtgagttt tgcaggacac agcgtacagg ttgttgcctg atccctttcc cggcctgtat
     3541 caacaggccg gttctctttt cagacttttg ccctctacgc cccgttcatc catgctgata
     3601 gcatcgacat tttgccgaag cgaccatcat gccaaccgtt attacgcacg ccgccgttcc
     3661 cctttgcatt ggtttagggt tgggttcgaa agtcatccct ccgcgtctgt tatttgccgg
     3721 aatcatccta gcgatgctgc ccgatgccga cgtattgtcg tttaaatttg gcgttgctta
     3781 cggcaatgtt tttggtcatc gcgggtttac ccattcgctg gtgtttgcgt ttgttgtccc
     3841 gctcttatgt gtgctgattg gacgtcgatg gttcagggct gggctgattc gctgctggct
     3901 gtttttaacc gtctcgttgc tatcgcacag cttactggat tcggtaacca ctggcggtaa
     3961 gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt ttcgctccct ggcaggtgat
     4021 taaagtcgcg ccgtttgcat tgtctcgtta caccacagcg tacgggcatc aggtgattat
     4081 ttccgaattg atgtgggtgt ggttgccggg gatgctactg atgggaatgt tgtggtggcg
     4141 cagacgataa ccggatgcga aaactcgcat ccggcaattg cgcaattact tacgacgcca
     4201 ggtggtccct tgcgggccat cttccagcac gatccccatc tcgttaagac ggtcacgtgc
     4261 cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc agacgctgtt gaattaacgc
     4321 ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg ctttgcagga acgcttccgg
     4381 ttcttgctcc agcaggccca atacagcgga aagtttacgc aggtgagatg ccattgcatt
     4441 cgccgctgcc atatcttctg ctttcagacg gttcacttca cgcgccatat caaacagcac
     4501 ggaataggct tccggggtgt tgaaatcgtc gtccatcgct tcaataaagc gcgcttcaaa
     4561 cgcttcgcca ccggcaggcg caacggtttt atctgtgccg cgcagcgcag tgtagaggcg
     4621 ctccagcgcc gcacgcgcct gcttcaggtt ctcttcgctg tagttcagct ggctgcgata
     4681 gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg tagtatttca gcacatcgcg
     4741 cacggtaaag aagttaccca gcgatttgga catcttctcg cggtcaacca tcaccattcc
     4801 ggagtgcatc cagtagttca catactgacc atcatgggca caggtggact gcgcgatttc
     4861 gttctcgtgg tgcgggaaca tcaggtctga accgccgccg tggatatcaa agtggttacc
     4921 cagctgcttg cagttcatgg ccgaacattc aatgtgccag ccaggacgac ccgcgcccca
     4981 cggagacggc cagctcggtt cgccctcttt cgacatcttc cacagaacga agtccatcgg
     5041 gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc tgcagctggt cgagatcctg
     5101 acgcgacagc acgccataag ttggatcggt cgggacgtcg aacatcacgt cgccgttgtc
     5161 cgccacataa gcgtgacctt tggcgatcag ttgttcagtg agttcaataa tttctgcgat
     5221 atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg ttcaaagcat caaaatcttt
     5281 gtgcatttcg gcaatcatgc gatccaccag cgccacaaag ctttcgccat tttcattggc
     5341 gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc acatacttca gtttatagcc
     5401 gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca aaggtacgcc cgtgaccgat
     5461 atgacagaga tcgtaaacgg tgattccaca cacgtacatg ccgacttccc cggcgtgaat
     5521 aggcttaaat tcctcttttt ggcgtgtcag agtattgaag atttttagca tcgaagattc
     5581 cgtttagaca tgtgtgggta attgagttgc gtataatacc catatttccc gcgcgaatca
     5641 gcatacattg cgtgatgatc cgatcgtgcg gttatgctat aacaccaccc tatatatgac
     5701 ccgaactggg ttgaagcacc aatcaaacgg aacaggatgc aaaaatggtt actttccaca
     5761 ccaatcacgg cgatattgtc atcaaaactt ttgacaataa agcacctgaa acagttaaaa
     5821 acttcctgga ctactgccgc gaaggttttt acaacaacac cattttccac cgtgttatca
     5881 acggctttat gattcagggc ggcggttttg aacctggcat gaaacaaaaa gccaccaaag
     5941 aaccgatcaa aaacgaagcc aacaacggcc tgaaaaatac ccgtggtacg ctggcaatgg
     6001 cacgtactca ggctccgcac tctgcaactg cacagttctt catcaacgtg gttgataacg
     6061 acttcctgaa cttctctggc gaaagcctgc aaggttgggg ctactgcgtg tttgctgaag
     6121 tggttgacgg catggacgtg gtagacaaaa tcaaaggtgt tgcaaccggt cgtaacggta
     6181 tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag cgtgaccgtt agcgagtaat
     6241 cgtggcgaca ctctttattg cagatcttca tctctgcgtg gaagaaccgg cgatcaccgc
     6301 cggttttctg cgttttttag cgggggaagc ccgcaaggcc gacgcgctgt atattcttgg
     6361 cgatctgttt gaagcatgga ttggcgacga cgatcccaac ccactccatc gccagatggc
     6421 ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt tatttcattc atggcaaccg
     6481 tgattttctg ctcggcaaac gctttgcccg tgaaagcggc atgacattat tgccggaaga
     6541 aaaggtgctc gaactttatg gtcgccgggt gttgattatg catggcgaca cgctgtgcac
     6601 cgatgacgcg ggttatcagg cttttcgcgc caaagtccac aaaccctggc tgcaaatgct
     6661 attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc gcgcgaatgc gcgcgaacag
     6721 caaagaagcc aacagcagta aatcgctggc gatcatggac gttaaccaaa acgctgtggt
     6781 cagtgcgatg gaaaaacatc aggtgcaatg gctaatccac gggcataccc atcgcccggc
     6841 ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc gtggtactgg gtgcctggca
     6901 tacggaaggt tcgatggtga aagtcacggc ggatgacgtt gagctgattc attttccgtt
     6961 ttaaaaaacc cacaactttg ctgatttcac agccacgcaa ccgttttcct tgctctcttt
     7021 ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc accacaggag ttttaagacg
     7081 catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg atggggtcca aaagcgactg
     7141 ggctaccatg cagttcgccg tcgaaatctt cgaaatcctg aatgtcccgc accacgttga
     7201 agtggtttct gctcaccgca cccccgataa actgttcagc ttcgccgaaa gcgccgaaga
     7261 gaacggttat caggtgatta ttgcgggcgc aggcggcgca gcgcacctgc caggcatgat
     7321 tgccgccaaa acgctggtgc cggtgctggg cgtgccagta cagagcgccg cactgagcgg
     7381 tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc attccggtgg gtacgctggc
     7441 gattggtaaa gctggcgcgg caaacgcggc attactggca gcacaaattc tcgcgactca
     7501 cgataaagag ctacaccagc gtctgaatgg ctggcgcaaa gcccagaccg acgaagtgct
     7561 ggaaaacccg gacccgcgag gtgcggcatg aaacaggttt gcgtcctcgg taacgggcag
     7621 ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca ttgctgtctg gccggtcggg
     7681 ctggacgctg aaccggcggc ggtgcctttt caacaaagcg tgattaccgc tgagatcgaa
     7741 cgctggccgg aaaccgcatt aacccgcgag ctggcgcgtc atccggcctt tgtgaaccgc
     7801 gatgtgttcc cgattattgc tgaccgtctg actcagaaac agcttttcga taagctccac
     7861 ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg agtggcctgc ggtgtttgat
     7921 cgtttaggtg aactggcgat tgttaagcgt cgcactggtg gctatgacgg tcgcggtcaa
     7981 tggagtttac gcgccaatga aaccgaacag ttaccggcag agtgttacgg tgaatgtatt
     8041 gtcgagcagg gcattaactt ctctggtgaa gtatcgctgg ttggcgcgcg cggctttgat
     8101 ggcagcaccg tgttttatcc gctgacgcat aacctgcatc aggacggtat tttgcgcacc
     8161 agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc aagccgaaga gatgctgtcg
     8221 gcgattatgc aggagctggg ctatgtgggc gtgatggcga tggagtgttt tgtcaccccg
     8281 caaggtctgc tgatcaacga actggctccg cgtgtgcata acagcggtca ctggacacaa
     8341 aacggtgcca gcatcagcca gtttgagctg catctgcggg cgattaccga tctgccgtta
     8401 ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc tgattggtag cgatgtgaat
     8461 tatgactggc tgaaattgcc gctggtgcat ctgcactggt acgacaaaga agtccgtccg
     8521 gggcgtaaag tggggcatct gaatttgacc gacagcgaca catcgcgtct gactgcgacg
     8581 ctggaagcct taatcccgct gctgccgcca gagtatgcca gcggcgtgat ttgggcgcag
     8641 agtaagttca gttaagcagg tgctcatttg attgccggat gcggcgtaaa cgccttatcc
     8701 ggcttaccgc tccggtaccc attgtaggcc tgataagacg cgtcaagcgt cgcatcaggc
     8761 atggtgcacc gattgccgga tgctctcatc actacagcga aatacaggtc cccgcttcgc
     8821 ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca cgcgggttta ctacggcttc
     8881 tgacataacc actcaccgcc gttaccttcg gccccatcga accatcggct ttggcaaatg
     8941 gcgctaactc atccggtgtg gcatggcgaa tggcacgttg ctgcggcgtt ccccagtttt
     9001 catataccgc atcagcatcg gtgaggatca ccagtccatc ggcattaatc tgctcggcga
     9061 gcaacgcagc ggcgagatct ttatcaatca ccgcttcact ccctgcccca tcttccgtca
     9121 caggcacacc gccgccgcca ctgcaaatca ccacatgccc ctctttgagc aacaactcga
     9181 tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc caccacccag cgcaaatatt
     9241 taccatcacg tttcatctgc cagccgtaag ccgcttccag tgcctcttgt tcttctggct
     9301 gataaaccgg accaataaat ttctctggct gcaaaaacgc cggatcatca ggcgaaacct
     9361 caatgcgcgt cagcaccgtc gtaacaggcg gcatctgcgg ctgtgcgctc aagctctgcg
     9421 ccagcatata gccaatcatc ccctggcttt ccgccaccag cacatccagc ggatacggtt
     9481 ctacctcttt ccacgccaga ttctgtaacg ccagcagccc cacctgcggc ccgttgccgt
     9541 gaacaatcgc cagccgataa gaacgggcca ggcgtgccag cgcgggtaca gcactggcga
     9601 tattgcgata ttgattttcc gccgtcagcg cctcaccgcg ctggagtaag gcgttgcccc
     9661 caagagctac aaccagtgtt ttcatggttt tccttgtaat aattgttggc caagccagaa
     9721 ccccagcagc gtatctgccc ctgacgtatg ccccagcgcc agcagcgaat caatcgcaac
     9781 agcggtacgt ttcgggcaac tcagagcatg gacaaagtgc aggagtggcg aggcgaaata
     9841 tccttgcgcg gcataacgta aataactgac gctcaccgct gtggtaacga gttgaagatt
     9901 gtcggaacag gcaaaaaacg ggcgaccggc gcgcgcatct aaagcgccat aataccaggc
     9961 cgccagcagc attccgctca acgtgtcatc atgacttggc gttaatccgg ggcctttacc
    10021 cagccagtgc cgccagtcgg tcttaacgcc attgagcgcg gcctgaaaac agtgacgaaa
    10081 ctggcgtaat tcagcgggca gcggatcgct tgccgccatc gccagtggcc cgaaaagtcc
    10141 ggtttcctcc gcgcgttgca tccatgcagc tgcaagcggt tgaggatgcg aaggcggcgt
    10201 aatacgcagc aaacaataac gctgtggctg tttaaccgtg aaacgcccga ggcgaatccc
    10261 tttagccaca acctgtggtc gttcattgcc gcataatcca ccgcataacg catcgaattg
    10321 cgtgcggcga aattacacat tcccgtcaag ctatagaaga aacgctgccc tggctttcct
    10381 cttttggcgc tgacccaacg ggtgggatga cccgtttact ttattcgccg gaatggctgg
    10441 aaacccagca gcaatttaaa aaaagaatgg cagcaagtgg gctggaaaca cgtttcgatg
    10501 aagtggggaa tttatacggt cgcctgagtg gcaccgaata tccacaggaa gtggttctga
    10561 gcggttcgca tatcgatacc gtggttaacg gcggtaacct cgacgggcaa ttcggcgcgc
    10621 tggcggcgtg gctggcaatt gactggttga aaacgcaata cggcgcaccg ttacgtacgg
    10681 tcgaagtggt ggcgatggca gaagaagaag gcagccgctt cccgtatgtc ttctggggca
    10741 gtaaaaatat ctttgggctg gcgaatcctg acgacgtgcg gaatatctgt gatgccaaag
    10801 gaaatagttt tgccgatgcg atgaaggctt gcggatttac tctgcctaac gccccactaa
    10861 ctccgcgtca ggatataaaa gcctttgttg aactgcatat tgaacagggc tgtgtgctgg
    10921 aaagtaatgg gcaatcaatt ggcgtggtga atgcaattgt cgggcagcgt cgctatacgg
    10981 tgacgctgaa cggcgagtca aaccatgcag gcaccacgcc gatgggttat cgtcgtgata
    11041 cggtttacgc tttcagtcgc atttgccatc agtcgatcga aaaagcgaaa aagatgggcg
    11101 atccgctggt tctgaccttt ggcaaagtag agccgcgccc gaatacggta aatgtggtgc
    11161 cgggtaaaac cacgttcacc attgattgtc gtcataccga cgctgccgtg ctgcgcgatt
    11221 tcacccaaca gttagaaaac gacatgcggg cgatttgcga tgaaatggac attggtattg
    11281 atatcgattt atggatggac gaagaacccg tgccgatgaa taaggagctg gtcgccaccc
    11341 tgacagaatt gtgtgaaaga gaaaaactga attaccgggt gatgcacagt ggtgccgggc
    11401 acgacgcgca aattttcgcg cctcgcgtac caacctgcat gatttttatc cccagcatca
    11461 acgggatcag ccataacccg gcggaacgca ccaatattac tgaccttgcc gaaggggtca
    11521 aaacgttggc actcatgctt tatcaacttg cctggcagaa ataaggagtc ataaatggga
    11581 tatttaaata acgtcaccgg ttaccgcgaa gatttactgg ctaaccgtgc gattgttaaa
    11641 cacggtaatt tcgcactgtt aaccccggat ggtctggtaa aaaatattat tccgggcttt
    11701 gaaaattgtg acgcgacaat cctctccacg ccaaagctgg gtgcctcttt tgttgattat
    11761 ctggtcacac tgcatcaaaa cggtggcaac caacagggct tcggtggcga aggcattgaa
    11821 acgttcctgt atgtgatctc tggaaatatc actgccaaag ccgaaggcaa aacatttgcc
    11881 ttaagcgaag gtggctatct ttattgccca ccaggctcct taatgacgtt tgttaacgcc
    11941 caggccgaag acagccaaat ctttttatat aagcgccgct atgttccggt agaaggccat
    12001 gcaccgtggc tggtttctgg caatgccagc gaactggaac gcattcacta tgaaggtatg
    12061 gatgatgtga tcctgctgga ttttctgccc aaagagttag gttttgatat gaacatgcat
    12121 atcctctctt ttgcaccagg tgccagccac ggttatatcg aaacacacgt tcaggaacat
    12181 ggtgcctata ttctttccgg tcagggggtt tataacctcg acaataactg gatcccggtg
    12241 aaaaaaggcg attacatctt tatgggcgct tattctttac aggctggtta tggcgtaggg
    12301 cgtggtgaag cgttcagcta tatttactcg aaagattgca accgcgacgt agagatttaa
    12361 gtcataccac tcgcctgatt atttttagct atgttggatt ttgccacggt ttataccgtg
    12421 gctttttttg taacattgcc tgatgcgctt cgcttatcag gcctacatga cctctgcaat
    12481 atatagaatt tgcgcgattt tcaggaagga tatagtactc atgccgcatc cggcatgagt
    12541 actgcgctct tatacgaaat aaatcgcatc cgctttaaag gcaagagtca gtttttaatt
    12601 ccctgaccta ttttaatggc gcaggcaata tttcgcgcgc tgttgaagag attggtttca
    12661 ccgctggcga gcacttcggc taaaggtgcc agacgaggca aaatgctgaa tagcgcgtca
    12721 atgccgtact ggtgcaccac ttccacgcca tcacccaata caccagcaat cccaatcacc
    12781 ggtacattaa actgcttcgc caccgacgca acgcccagcg gcgctttacc gcctgccgtt
    12841 tgcgagtcaa tgcgcccttc cccggtgatc accagtgctg ccccctgcac cgcctgcgca
    12901 agattgaccg cattcaacac aatctcaatg cccggtttaa tatccgcatt gagaaatacc
    12961 gccgccgcaa tccccatacc gcccgcagcg ccgccgcccg ccatctgaca gacattaatt
    13021 tcagtttgct gttgtaaaac acgggcgtaa ttttgcagcc cctgttccag ctcttcaacc
    13081 atctccggcg ttgccccttt ttgcgggcca aataccgccg ccgcgccgcg tgcccctacc
    13141 agcgggttat caacgtcaca tgccacttca atatggctgt tcgccagacg cggatcgcat
    13201 tcctccatct caatgcttgc cacgcgcgct aaattgccgc cgttagccgc cagcacctgg
    13261 ccgtcggcat caaggaaacg cacaccaagc gcctgcgcca tgcccatacc gccgtcgacc
    13321 gtcgcactgc cgccaatgcc gagaataata tggcgaatgc cgttatccag cgcatggcga
    13381 attaactccc ccgtaccaaa actggaggcc agcaacggat tacgcttttc aggcgcgacc
    13441 agcatcaggc cacttgctgc cgccatctca atcaccgctg ttttcccgtc tccggtaagg
    13501 ccataaaaag cattcacttt ttcgcccatc ggcccgcaga cttcaagcgt cacgatgttg
    13561 ccacccgtcg cggcgaccat cgcatccacc gtcccttcgc cgccatccgc tatcggcaaa
    13621 cagatatagt gcgcatcggg aaagagggtc gaaaacccgg ctttaattgc ctgacaacat
    13681 ttttctgcac ttaagctctc cttaaaagag tctggcgcaa tgacaatctt cattatcaat
    13741 cctttttcaa caaattattc gatgcgttcc cacggaataa gattatccat aagaacagcc
    13801 agtaaaattc cgaccagtaa gccgttactg agcagaggac gaagcgttaa tggaaggtct
    13861 tgcaaataca caggcggtaa tgccattaaa aatatgccga caaataacgg caatgcgagt
    13921 cgataaatat tgcgctcggt aaacgttatt tgctg
//
LOCUS       DAJVQH010000106        13939 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJVQH010000106 DAJVQH010000000
VERSION     DAJVQH010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.106"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..106)
                     /locus_tag="OQM87_003135"
     CDS             complement(<1..106)
                     /locus_tag="OQM87_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCS1509186.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(147..602)
                     /gene="csgB"
                     /locus_tag="OQM87_003136"
     CDS             complement(147..602)
                     /gene="csgB"
                     /locus_tag="OQM87_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCS1509187.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1356..2006
                     /gene="csgD"
                     /locus_tag="OQM87_003137"
     CDS             1356..2006
                     /gene="csgD"
                     /locus_tag="OQM87_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCS1509188.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2011..2400
                     /gene="csgE"
                     /locus_tag="OQM87_003138"
     CDS             2011..2400
                     /gene="csgE"
                     /locus_tag="OQM87_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCS1509189.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            2425..2841
                     /gene="csgF"
                     /locus_tag="OQM87_003139"
     CDS             2425..2841
                     /gene="csgF"
                     /locus_tag="OQM87_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCS1509190.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSVLQNNSTDF"
     gene            2868..3643
                     /locus_tag="OQM87_003140"
                     /pseudo
     CDS             2868..3643
                     /locus_tag="OQM87_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3707..4198)
                     /locus_tag="OQM87_003141"
     CDS             complement(3707..4198)
                     /locus_tag="OQM87_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCS1509191.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="OQM87_003142"
     CDS             complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="OQM87_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCS1509192.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="OQM87_003143"
     CDS             complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="OQM87_003143"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCS1509193.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="OQM87_003144"
                     /pseudo
     CDS             complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="OQM87_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6842..6929
                     /locus_tag="OQM87_003145"
     tRNA            6842..6929
                     /locus_tag="OQM87_003145"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6876..6878,aa:Ser,seq:gga)
     gene            complement(7079..7360)
                     /locus_tag="OQM87_003146"
                     /pseudo
     CDS             complement(7079..7360)
                     /locus_tag="OQM87_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7642..8646)
                     /locus_tag="OQM87_003147"
     CDS             complement(7642..8646)
                     /locus_tag="OQM87_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCS1509194.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8718..9278)
                     /locus_tag="OQM87_003148"
                     /pseudo
     CDS             complement(8718..9278)
                     /locus_tag="OQM87_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9363..9560)
                     /locus_tag="OQM87_003149"
     CDS             complement(9363..9560)
                     /locus_tag="OQM87_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509195.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9572..10063)
                     /locus_tag="OQM87_003150"
     CDS             complement(9572..10063)
                     /locus_tag="OQM87_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509196.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10060..10434)
                     /locus_tag="OQM87_003151"
     CDS             complement(10060..10434)
                     /locus_tag="OQM87_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCS1509197.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10481..10855)
                     /locus_tag="OQM87_003152"
     CDS             complement(10481..10855)
                     /locus_tag="OQM87_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCS1509198.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11018..11239)
                     /locus_tag="OQM87_003153"
     CDS             complement(11018..11239)
                     /locus_tag="OQM87_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCS1509199.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11302..11778)
                     /locus_tag="OQM87_003154"
     CDS             complement(11302..11778)
                     /locus_tag="OQM87_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCS1509200.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11794..12267)
                     /locus_tag="OQM87_003155"
     CDS             complement(11794..12267)
                     /locus_tag="OQM87_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCS1509201.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12609..13430)
                     /locus_tag="OQM87_003156"
     CDS             complement(12609..13430)
                     /locus_tag="OQM87_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCS1509202.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13530..13703)
                     /locus_tag="OQM87_003157"
     CDS             complement(13530..13703)
                     /locus_tag="OQM87_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCS1509203.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
BASE COUNT         3576 a         3413 c         3334 g         3616 t
ORIGIN      
        1 cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac
       61 cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc
      121 ggattgattt aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac
      181 tgtctctgca ctacaattgc cgttttttga gtaccatact gtgtaatatt tgctttatta
      241 ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga aatactggca
      301 tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc
      361 tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc
      421 cgtaactgag cactattatt agtcccagct tgaccaatta tggctgcctg attaaatgaa
      481 gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct
      541 gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc
      601 atgttgtcac cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt
      661 taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat
      721 ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa
      781 cccgcgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt
      841 ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
      901 tgtcttgcgc acaagccgtg acaaacctcg tctttttgca gtaaaaaaat tgtccacgga
      961 ggtgtggaga aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatatttttt
     1021 atatgtatta ttagtaagtt agcaccattt gtatgatttt ttaaaattgt gcaataaaaa
     1081 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaatgt taacatttta
     1141 atataatgag ttacattttg ttacatattt aacacttgat ttaagatttg taatggctag
     1201 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
     1261 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
     1321 caggtgtgcg atcaataaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc
     1381 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
     1441 accttaaaca atcgctagca attacaggaa aattacataa tattcaacgt tctctggacg
     1501 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
     1561 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
     1621 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
     1681 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgtggcgaat
     1741 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
     1801 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
     1861 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
     1921 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
     1981 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
     2041 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
     2101 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
     2161 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
     2221 agctggatca caataacggt caatcaggat gttattttcc agactttttt attcccgttg
     2281 aaaagagact tcgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     2341 cgtcgccaga taaatcaggc gttattaagt acgggcgatt tggcgcatga tgaattctaa
     2401 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     2461 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     2521 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     2581 gatccgagct ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact
     2641 caggccatcc agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg
     2701 ggccgcatgg tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag
     2761 ttgaacgtga cagatcgtaa aaccggacaa acctcgacca tccaggtttc ggttttacaa
     2821 aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat
     2881 ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg
     2941 ccagaccgac attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc
     3001 cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac
     3061 cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccagggct tacaaaacct
     3121 gcttaacgag cgcaaggtta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa
     3181 ccgaatcccg ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattattgg
     3241 ttatgaaagc aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga
     3301 cacgcaatac cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg
     3361 tgagatcctc tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg
     3421 ggttttccgc tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa
     3481 cgaacccgtt atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa
     3541 tgatggtatc gaccgtggtc tgtgggattt gcaaaataaa gcagaacggc agaatgacat
     3601 tctggtgaaa taccgccata tgtcggttcc accggaatcc tgacagataa aaatgccgga
     3661 attaataatc cggcatttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt
     3721 tgatttcctg ctcacggtga gccgtctttg cactacgcgc cgccagccac aggccgctgt
     3781 ttttcattgc gtaaccaaat accagcccaa gcgccagcga aggtaacacc agtttccagt
     3841 caccctgccc ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca
     3901 gcagctgttt ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga
     3961 gaatttccag atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc
     4021 cactaagcag ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaagcaa
     4081 agtaggccgt acagcccagg aagcccgccc agcttagtaa acccagggaa acagccaccc
     4141 atccccagat accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc
     4201 cgcgcatgat agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa
     4261 caaattatct gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc
     4321 cagctcgtcc cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa
     4381 aggcgtggtt gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata
     4441 ctcactgaac agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca
     4501 ggaagccgca agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtggcatccc
     4561 tcgttcggaa agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg
     4621 atatggcggc acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg
     4681 cgtcatatca cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt
     4741 cgcagccagt ttcccctcac gaatcagagt aaacagcggc accagtaaag gatcttgcgg
     4801 ttggcggtaa tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat
     4861 atttccatta ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat
     4921 tcaaggaagt tcagtaagcg gcgcggagaa acattcaaaa tgcgctctgg cggaaaatct
     4981 accgcgtcga ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa
     5041 tccgagccta atgccaccca acctccagca tcacgtaccg ctgcagctac cgcacgacag
     5101 ttgtcttcac tgcccttacg tgagtgtaaa aatgaggaat tattgatttc cagcgccacc
     5161 tgatgtttcg cggctgcttc agcaacggct ttaacatcta tttcatattt cggatttccg
     5221 ggatggctta ttatatgcac attgccgctg gcgatagtgg cgatcatcgc ttgtgtattg
     5281 gtcgctttgt catgtggcgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc
     5341 gagtcaaaca ttttaccgct gcagtcaatt tcaccatcaa catttttaat gttagcttca
     5401 atgccgcgca ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag
     5461 tgccagtgat gcggcgcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta
     5521 atgccctttt gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg
     5581 gcaacggtat gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg
     5641 ccaaggatag caggaatcct gatgctttat tagtagccgc gtgcgcggtc gacttgcccg
     5701 cagaccctct cccctttttc gagctgggca atagtgcgag aaatgtactc cacagcttca
     5761 gcgggacggg taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc
     5821 ggactttcag gcggtaaggg ttcacgatta aaaacatcca gcattgcgcc tttaactttc
     5881 ccgctatcca gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg
     5941 aggagatacg cgccatcagg taatttttcg agtaattgtt gattaataat gccgacggtt
     6001 tcaggggtat tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt
     6061 tcttcccgtc cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa
     6121 cgcagcggaa agcgctaggt ttgcagactc tgagcaactt tacagcccag tacgcctgcg
     6181 cccaaaatgc cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa
     6241 ctattttgct ggatgcgata atcatcaaaa cgtcgaaacc aatgcagcac ctgactgaca
     6301 gcatattcct gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa
     6361 gggttcagca tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg
     6421 agtgcgaaca ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact
     6481 aaaacataat cagcagagtc attatctccg cttttccatg ccctgactct tgcctgagga
     6541 atagctttgc gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg
     6601 atatccatac tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat
     6661 ctacaaaggc caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt
     6721 attaaaacca cacattgatt gaagtttgaa taaacgcgcg attctttcaa aaagtttgtt
     6781 gacctcaggt catgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt
     6841 tggtgaggtg tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat
     6901 cgggggttcg aatccccccc tcaccgccag attataagtg catgttattg tttaacatgc
     6961 gcttttttat ttttccttcc agacagtatg ccatcgggta tgccatctga aaccggttta
     7021 tatgccccgc gatcatatct tatcggacaa tttccgatct ttgagcatac tggcaaagct
     7081 atactgccag catatcagga tagtgttttg cgataatatc attcagtctg tcaaccagtt
     7141 ctggttttct gaacgtgata tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct
     7201 catcttcata gctctcttta ccctgaacag actgaatgtg atcatccaga cgaagggtga
     7261 tgtttccccg gttgtcgggg attggtttac cgctgaacag gtgcaacatt ctttccagat
     7321 cggcgagtcg gtcagcctgt tgtccggtga taagatgaaa tctatggtca cccccgtttt
     7381 tgcaacactg atttttgatt aatggttggc ttgcttaaat ctatccggcg tctctgtggg
     7441 caaggatgcc cgcgccccga tgagttccgc acctgataat cctaaaaatc ccctcggctt
     7501 cagtgagcca tttcatctgt taggttttta atcaggtcga tttgccgttc atgtcatcaa
     7561 taatcagtct tcgcaaaacc agaaaggtaa cttgtatctt attgtttaaa ataaactaat
     7621 tgagttatat cagcatctat atcaggcctg atattctttt ttctgagtag tgattgccca
     7681 ggcaattcga gccagtttat ttgccagagc gcaggtaacc acattcgaat gcttcttgtt
     7741 gagttgctcc cgaacccact cagcaagttt tccatgcttg cgttcaagtc gcattatgaa
     7801 tgagcgggca cactgaacca gtaaccggcg taaattttta tcacctcgct tactgatacc
     7861 caacagagtc gattttcctc cagtgctata ctgccgggga acaagtcctg tggaagctgc
     7921 aaaatccctg ctgcatgaga actgtttccc gtcaccaagt tgcgacgata gtaagctggc
     7981 agtaatcgga ccaacaccag gaattgtcat aatgcgctga gcggtatcat cagcgttgat
     8041 tgactgactt aattctgatt ccagctctgc tatttgctca acaaggtaaa agtagtgggt
     8101 atgcaacctc accaaaaggc ggctcagata ttcggggatt tcgtgttcag ccaacacgag
     8161 agacaaccgt tttattacag catcacctat cggcaggctg attccaaatt ccagcaagaa
     8221 accgtggatc tggttggttg ttttcacctt atctctgatg agcgattctc tgacccggtg
     8281 taacgccctc atggcctgct gggtctcagt tcttggctgg acaaaacgca ttgatggtcg
     8341 tgaagccgct tcgcatatgg cttctgcatc aacaaaatca ttcttattac ttttgacaaa
     8401 cgggcgaacg aactgtggag aaatcagctt agtttcatgc ccaaaatcag agatacagcg
     8461 agccataaag tgtgcacccg cacaggcttc cattacaaca actgagggcg gacatgtcgc
     8521 cagaaactgc atcagtttag tacgggtaaa ttttttacgc agaagagcct taccagactt
     8581 gtcttgacaa tgaatgtgga aggagtgctt tccgagatcg ataccaataa gtgtgatgtt
     8641 ctgcatgatg gtctctccgt taaagggatt taccttaaca gcgtactgct gttaaggtgg
     8701 gggtgaccat atcattatcc ccaccggtcc catctcacca gattgttgac gataatttta
     8761 ctgccaaatt tacaggggca attggttttg taattccagt tcagccctct gaagacgttg
     8821 atcacccctc gctcaaacac ctcatcctta ttctggtgca gttgttcaaa tgtgctcaga
     8881 atgttcgctt cgctgatttc cggaaaatta tcgtactcca gtgaccggta ccatgtatca
     8941 cgggcctgcg tatccattaa tgacagcatc ccggaacgtt gcatcaggtc acgccagatt
     9001 tcgcgatcaa gattccgggt aatggctttc attgctgttt cgggtttctc catcagccag
     9061 cagccgcagc gaaaatcctg tttcatggcc cagtccgggg cagttttacc tccgatgctt
     9121 tccgtcagca ccgagatttc agccagttgt cggataagtg tgtcgatttg caacagtgta
     9181 ctctttcgta cagccagaat gtggcgaatg ctggttgagc agataacgtc cgtatgcccg
     9241 ttgaccactt ccggacggga gattgtgata tctgacataa tcgatattcc tttttgcaga
     9301 taaatgaaaa cgccagccct gtgcaggctg gcgtcagagg aatgaagtct ggtgagtgag
     9361 cttcaccggt tctggtgcag aagctgcaga tgacgcaggg tacggacatc ctcccgggcc
     9421 tgttcgatac agggcaacag tgctgctgaa tccgttttat cctcatcgtt gtcaaagata
     9481 attcccgatc cgcagtcgat attgtcctgc agccacgcaa tcagaatatc cagcgctgtt
     9541 tccgtggtta atgatttcat gttgttcctg gttaccagtc gaaagtgggt aaacccggca
     9601 ggcattcagc gctggcatcc agatgaatga gactgacgcc ataacgccgg atgagtgtga
     9661 cgaccagacg acggaacgtt tttgacagcc ccaggcgttt aagccgcaga accgggtatg
     9721 accacgcatc ggtacgtaac agatatccgc tacctgtaaa atgaatccat tctgattcac
     9781 cgaagtcact ggtctggtgt gacagcgagt acaaccaggc gttgtccgtt tccgtgatat
     9841 gtgcggtact gcagcgtatg ccggtaaggg tagcaaatgg tggcgtgcat tctgctgagg
     9901 tctggtcaga ttcatcaaca acacgaaggg agtagccgtt atcgcagacc atgttaatca
     9961 gttcagcgag ctcaatacca tcgacgtcaa cgacaatgcg ccctatgttc agtgcctgta
    10021 cgttaacgct gtcggcttcc agtgtcaggg ccagtttcat catttcgcct ccggatactt
    10081 acccagggta atgttattta ccgttctgta attgtcgcgg gtcatcaggc cggtcgcccg
    10141 gcgagcccgg aggatatcaa tactgtttat taactgagag cggggacagg tgctgaatcc
    10201 cggctggtcg gtacgcacca gcgcgtattt ttccacgaga aaattaactg catcacacag
    10261 tgaaatgcct gcctcaatat gctgctcaat cacacgttca tcgacaaacg gtgtgtcatt
    10321 cagtgtgagg ccataatgct ggtccagcag tcggatgagc agtgtctgcc agatggtgac
    10381 gggagacggg cagcgagata cctcccgtac atgtgtgtcg gataatgttt tcatcttaag
    10441 ttctctgtaa ctggtagtgc taaaggtgag agcttcgggg ttatgaggtg gttgcgggtg
    10501 ctggtgtcgg ataaacagcc agataaacgt aaccacagga gccgagggtg tcggcttcgc
    10561 aggtcagccc ttttgcatac agcgtgacgg tatgctgata tcggggattc agttcaccgc
    10621 tggtgagcat gagttccagt tgtttcatca gcagcggaaa ggcctggtcc agatggtatg
    10681 catccgcatt gctgaaccgg cctctgatac cggcgcggtc tgcaaggtaa tacaggcggt
    10741 taccctcttg caccagacga gcgccaaaac agggcctcac tgtgcaggga agcccccacc
    10801 aggggcggtc gtgattgtca tcgggatgag ttatcccgga gagtttgtct gacacgataa
    10861 aatcctctct gaaaagtggt taagaatggt ttaacgatga caggtcagcc ggcgcgctga
    10921 ccggtttcgg tgaagatgta tgcattgatg agaatggcct catccacagc ggcgtctgag
    10981 gtcatctggt cgtactcgtt tcccgggtga cggaacatca gttcagggtg acactcatca
    11041 gccggacata cgggccaaaa ctgtccttac ggcgttctgc aaacacggca agaacgccgg
    11101 gaatatcctg cacttcacga ccagtatacg cttcagcact gccgtgccag cgatacttac
    11161 cggtacagaa cggaaaaaga cgggatgcag gatgctgttg gtgaatacgc atggcttcac
    11221 cacgggtgat gattctcatg atgtgatacc tctgaagaca gaagataaaa gtgaaaacag
    11281 gtgtgatgtg gttgtgacgg gttaaagcag accatgttcg gcaaaggaga aaacctggtt
    11341 gccaccgact atcagatggt ccggcacccg gatatccacc aggcccagtg cctgtaccag
    11401 acgttcggtg ataagccggt ctgccttact gggtgtgact tcaccggacg ggtgattgtg
    11461 cgccagcacc acggcagcgg cattgtggta cagggcgcgt ttaatcactt cccggggatg
    11521 aacttccgta cggttgatgg tgccggtgaa gagggtttca ccggcaatca gctgattctg
    11581 gttgttcaga tacagcaccc ggaactcttc ccgctccagt cccgccatgt tcagaatcag
    11641 ccattcccgt gccgcacggg tggaggtgaa ggccacgccg ggttcatgaa gatggcggtc
    11701 cagggtttgc agggcccgca gaatgagact gcgctcgccg ggcgtcatgt ttccgggcag
    11761 aaaggaaagc tgctgcattg tccctgcctc cattcagtcg atgatgcgca taatggcgct
    11821 gcattccgga tgctgcaggg cgtaatcccg cagccggtaa taatggaccg tcatggcata
    11881 acactccgta cgacaggcat gatgactgta cgtcatcaga caggcagcaa tgccggcggc
    11941 ttccgggctc atttcagcgc ggttaccgtt catggcattg aacagtaccc atgtttcgtc
    12001 atcatcgtca tccggttcgg gtgccataaa tgccccgccg ttgttcaggg tgtacagatt
    12061 ccagatacca ccgcagtagt cttcacacag acggtccatc cagccgaaga cacggggttc
    12121 cagggtgacc cactgtggaa tgaggccaaa atgctgcggc cagaagctga tgcgctgttc
    12181 atcagggacg ggagtggcaa ccagttgttg caggccagtc tccggtgcac aggccgaagc
    12241 ggtggtgttc tgagacaatg ttttcatgta tgaaatcctt gtaatgtgaa taaatgagag
    12301 ggagtaaagg tcgggagctg gcaacatcac gttcatcact gaccgtgatg acccggtaat
    12361 atcagggatg aatgatattc ggctgaaatt ccggacagcc gtacgcgtaa ccgttccggt
    12421 cacagacgga tggccagatg ccattgcctg tatactcagt ctgcacagac tgatttctcc
    12481 gggacggtaa agcagacggg tacaccaggc gcacaggtca tcatcagaaa tggaacgaca
    12541 ccccggcagc gcctccgggg cgtcgggtga ttctggtgcc tgaatcacgc cttcagggac
    12601 atgctgcctc agcgcaggct ctccagcagc gtttctgcca ttacccacaa tgcgcggttg
    12661 agcttaatat cggtgtcgat gttgtgaatg gcacgggtgt ggatacgttt tccttttgca
    12721 ctgcggccgg aaatcccgcc tttcagcata ttctcctgga tggtctgata tgcgctccac
    12781 aggtctttac cgtaatcctc cctgcgtcgt ggtgtcagaa tgtcagcggt ggtgactggc
    12841 tgatgttcgt caccataacg gtaagtcagt gccgcctgtg ccagcgcctg gcgtgccggt
    12901 ggcggcagga ccagcgactg catggcatca cgcttctcct ctatccggtc aaacacgccc
    12961 accacctcgt aagccccttc aataactttc tccaccacat ttccccggtg tggaacacgc
    13021 aattctccca gagactgacc gcagaccagt ccattggtac aaatggcacg aaaatatccg
    13081 ggtaacatct ggtagctgga tgaaccgtca tgagagttga gcagaataat ttcagggaca
    13141 tgctgaccgg ttatctgtcc ggcccgccgc agacgcagca tgtgttttgt gtattcccgg
    13201 cgacccgggt cacgcacacg tgtctggcag gcgaagaatg gctgaaagcc ttcccgctgc
    13261 aggctttcca gtacggtgat ggtgggagtg tacgcatagc gtttactgcg ggaagtatgc
    13321 cggtcttcac cgaaaatact gggaacatag tgcatcagtt cttcgtgtgt cagcggacgg
    13381 tcacggcgta tctggtttgc agcataacca aaacgactgg ctagtcgcat aatttgctcc
    13441 ttatcggtgg ttaagattta ctggtgtaat aaatgaaaaa gccacgtctc ccggagaaga
    13501 cgcggcctga tggacgaaat taatgaggtt tattgtctga gaagaccgtc actggcgaac
    13561 cttccggcgt cagtctctgc gttgcggata acccgacgga aatgagttcc ctggtcatcg
    13621 tcgataaaca cattgcggta agctgttgtg atgacaacag cctgctcgcg tgagaaaggg
    13681 acttcgggca ataaacgtga catacaacca gacctgtatt tggttccctg aaaatataag
    13741 tccctgtcat catggcagag ccgtatttac ttttgatatc aataaattaa taacgcgaag
    13801 atgaaccgca taacttacgc ctttaatttg ttttgtttac gtagtccctg accaggtacg
    13861 cgaattcgtg aacgccagtt tcaccaaacc aaatcaggta accagggtcg atgttgtaat
    13921 agtaaccaac gactttatg
//
LOCUS       DAJVQH010000107        13766 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJVQH010000107 DAJVQH010000000
VERSION     DAJVQH010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13766
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.107"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(184..1437)
                     /locus_tag="OQM87_003158"
                     /pseudo
     CDS             complement(184..1437)
                     /locus_tag="OQM87_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1544..2437
                     /locus_tag="OQM87_003159"
     CDS             1544..2437
                     /locus_tag="OQM87_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1509204.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2572..3792
                     /gene="mlc"
                     /locus_tag="OQM87_003160"
                     /gene_synonym="dgsA"
     CDS             2572..3792
                     /gene="mlc"
                     /locus_tag="OQM87_003160"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCS1509205.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3917..4612
                     /gene="bioD"
                     /locus_tag="OQM87_003161"
     CDS             3917..4612
                     /gene="bioD"
                     /locus_tag="OQM87_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1509206.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4565..5857)
                     /gene="clcB"
                     /locus_tag="OQM87_003162"
     CDS             complement(4565..5857)
                     /gene="clcB"
                     /locus_tag="OQM87_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCS1509207.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6016..6630)
                     /gene="dmsD"
                     /locus_tag="OQM87_003163"
     CDS             complement(6016..6630)
                     /gene="dmsD"
                     /locus_tag="OQM87_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCS1509208.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6673..7527)
                     /locus_tag="OQM87_003164"
     CDS             complement(6673..7527)
                     /locus_tag="OQM87_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS1509209.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7529..8146)
                     /gene="dmsB"
                     /locus_tag="OQM87_003165"
     CDS             complement(7529..8146)
                     /gene="dmsB"
                     /locus_tag="OQM87_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1509210.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(8157..10427)
                     /locus_tag="OQM87_003166"
                     /pseudo
     CDS             complement(8157..10427)
                     /locus_tag="OQM87_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(10436..11102)
                     /locus_tag="OQM87_003167"
                     /pseudo
     CDS             complement(10436..11102)
                     /locus_tag="OQM87_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            11085..12685
                     /locus_tag="OQM87_003168"
                     /pseudo
     CDS             11085..12685
                     /locus_tag="OQM87_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397936.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            13179..13547
                     /locus_tag="OQM87_003169"
     CDS             13179..13547
                     /locus_tag="OQM87_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509211.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
BASE COUNT         3448 a         3511 c         3519 g         3288 t
ORIGIN      
        1 gcgcatcagt aataaatgac acacagcaaa atgaatccgt ttatttgggt acttataatc
       61 ctgatgacgc tagatgcgct gcgggaattg gctggcgctt cgtctatttt aggatggcta
      121 ttaacgttgg tttgagcagg caataagtcc ggacgggtat ttaccgcagt ccggacttat
      181 ttttcaggcg tgcagacgac gatgcaaacg cgccccgacc agcagagcaa tgaccagcat
      241 cagagcaata aatgcgccga cgccgttcca gccatagtta tgccagaaaa caccacccag
      301 cgtcccggca atactcgacc ccagatagta actgaacaga tacagcgagg aggcctggcc
      361 tttagcgcgt tttgcgcggg ggccgatcca gctgctggct actgagtggg ctgcgaagaa
      421 tccggctgag aagagtaaca ttccggcaaa gatcagccac agcgagctaa ataaggtcat
      481 cagtaaacca aacggcataa ctcccgtcga aaacaacatc accggaccgc gcccatagcg
      541 ggtggtcatg gttccggctt tgggtgagct ccatgtaccg gtcaaataag cccgcgataa
      601 taagccaacc acggcctgac tgacatgcta gggggaaagc atcaaccgat agccgatgta
      661 attaaacagc gtgacgaacg accccatcag caaaaagcct tctgcgaaca ataacggtaa
      721 tccccggtca cgccagtgca gacgaaagtt gataaataac gtcttagggc gcagcgaagt
      781 cgggcaaaaa tggcgtgatt cagggaggat tttccagaac atcaacgccg aggccagcgc
      841 gaaacaaccg attgccgcca gagcaattcg ccagttgaaa aagtccgtga agacaccact
      901 aattaagcgt ccgctcatgc cgccaattga gttgccgctg atatacaacc ccatcaaaaa
      961 ggccacgaaa ctggggggga tttcctcgct aagataagtc atgccaacag ctgccacgcc
     1021 acttaacgaa agcccaatca aggcgcgcat aatcaaaatg ccgtgccagc tggtcatcat
     1081 tgtcgaaagt aacgtacaaa tggaggccaa cagtagcgcc gtgaccatca ctggtttgcg
     1141 accaatggca tcggatagcg ggccagtaaa tagcaaaccg atagccaaca tcgccgtgga
     1201 aatggacagt gaaatactac tgttcgcggg cgttaagcca aactcctgcg aaagcaccgg
     1261 aaggataggc tgcacacaat agagaagtgc aaaggttgcc agtccggcag agaacagcgc
     1321 cagggtgacg cgcataaatt gcggcgtacc gcgtttaata aattgatttg gctgagaaat
     1381 gctttgcttg tcagtgtcgc ttgccggagc gccatcaaca gttgtagtac ggctcacttg
     1441 aaatccttgc taaatatgcc tgtggatcag gcttatacat agggtaggaa aatcgaattg
     1501 ttctgtctaa tatattaata atctcaaata agatgtttta aatatgaata ttgaacttcg
     1561 tcatctgcgt tactttgttg ctgttgcgga agagctgcat tttgggcgcg cggctgcccg
     1621 cctgaatatt tcgcaaccgc cgctaagtca gcagattcag gcactggagc aacaaatcgg
     1681 tgcccgactg ctggcacgaa ctaatcgcag tgtattgctg acggcagcag gaaaacagtt
     1741 tcttgcagat agtcggcaaa tcctgtctat ggtggatgac gctgccgctc gtgctgaaag
     1801 actgcatcag ggtgaagcgg gggagttgcg tattggtttt acttcgtccg ctccttttat
     1861 tcgggcggtg tccgatacgt tatcgctgtt tcgccgtgat tatcctgatg tccatttaca
     1921 aacccgcgaa atgaacactc gcgagcaaat cgctccgctc attgaaggaa cgctggatat
     1981 gggattgctg cgtaacacag cgttaccgga gacgcttgaa cacgcagtca tcgtccatga
     2041 accgcttatg gcgatgatcc cgcacgatca tcccctggca aataatccga gtgtaacact
     2101 ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga ctgggctgta
     2161 tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca ctcaggaggt
     2221 gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt caattttgcc
     2281 tgcgtcattt aaacgtgttc agctcaatga aatgcgctgg gtgccgattg ctgaagagga
     2341 tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc cagctgcgcg
     2401 taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc gaaaaagccc
     2461 gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta ttgaagtgct
     2521 tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc ggtggttgct
     2581 gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc ggtttatcgc
     2641 ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc gcagctggct
     2701 cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt gcgagagctg
     2761 gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt tgaaactgaa
     2821 gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc tctgcgcgat
     2881 ctgagcagca aactggtggt ggaagagtcg caggaattgg cgttaaaaga tgacttgcca
     2941 ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca gaaaaaactt
     3001 gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga aaatggtatt
     3061 gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga ggcgctggag
     3121 cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac gatggcagag
     3181 gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat cgatcacaac
     3241 gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag tagtctcgtg
     3301 gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg gaaccacggc
     3361 tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct gcgtctcaat
     3421 caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt gtgtcaggcg
     3481 gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc gcatgtaggg
     3541 cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat tggctcaccg
     3601 ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg tcagcaggcc
     3661 cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa ccagggcacg
     3721 atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt gattcgtctg
     3781 ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca atttgggcca
     3841 ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt tgtgaagcat
     3901 aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca cttctgtagg
     3961 gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa aaacggttgc
     4021 gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc gtaataaaga
     4081 tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag ttaatcctat
     4141 cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt acaccctcat
     4201 ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag aagggactgg
     4261 cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag tgcaggaaca
     4321 actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg cactgctaac
     4381 agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta accgaatcaa
     4441 cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac ttccggcacc
     4501 gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg ggcaatacat
     4561 ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt aacgtccgcg
     4621 aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga tactccccgg
     4681 tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc gccaacagtg
     4741 tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg aaccataatc
     4801 ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc ggtgtaaaga
     4861 caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta caaaggaaga
     4921 tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg gtgctatagc
     4981 cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac ccgcctagtg
     5041 ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag gcgttcatta
     5101 acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata atcagcgcat
     5161 agtcacgagc ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg tcgctatgat
     5221 taatcagatt gctaaccagc aatgccacga cggcggaaat aatcaccggg ccgagagagg
     5281 ccaacatcat agtgccaaac agcacttcgg ctataaataa actgccagca agcggggcac
     5341 gataggccgc agccattccc gccgcggccc cacaggcgat ccataatttc cattcctggc
     5401 gtggcgtaaa acgttgggca aaacaggagg cggcaagggc agctaaaaga atcatcgcac
     5461 cttcgcgacc aattgcactg ccgctggtta ctaccagcag cgaggcaagc gatttaacca
     5521 ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa tcggtcggcg
     5581 catgagggcg ttgttgggta aatttctgcc agcccatcag caacaaaccc gccgccagtc
     5641 cgccgagcgc cggagttagc agccgtcgcc agggggaaag gtttgtcgct gcattgacca
     5701 gactgccgga gtcattattg aggaacaacc actccagtag cagcatcgca tgacgaaacc
     5761 cggcaacggc aaagacagcg agaataccga cgactgttgc gataagcaga cggcggaaca
     5821 tagtgcgcag atccggatag atgtgtagat gatgcatgag cagaaatggg caatgaaaca
     5881 cgggcgacta ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc tttaagagat
     5941 aaaaaaacgt gacattgtaa cgtattattg ccgaatcgcg cgtaatcgtc accatccggc
     6001 aatattacgg tgatcctatc gaaagagtgg tttaaccgcg acaggaatca acagttgtga
     6061 ttgccattgc gccagcgtta agcgagccag ttcacccagt gcacggtaga aagggtgttc
     6121 tgctttttcg ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt gccatgccag
     6181 caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca tcaacagcag
     6241 tgaaccaaaa tgatcttccg gttcgttttg cttcatttca aactgaatgc ctttctcgcg
     6301 catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc gatccagcca
     6361 gaccgacccc cacggcggag acggcagtgc ccacgggccg acaaacaaac gctgccaggc
     6421 ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc accagcggcg ctaatgacgc
     6481 ttctggtaga ggccactgag tttcccagcc gtcactggtg agtaccgcaa ccagaggtgc
     6541 ggcttccgca ctctctggag cgtaataaaa cagcgctccc aacacgcgcg ccgcgacaga
     6601 aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg cgggttgccc
     6661 cgcaccaatt tgttaacctg caatagccat tcctactgtc atgtgcaggc cataaaagag
     6721 cacacgcccg atcatctcac caacaagtat gaggattagc cccagaacaa gtccggcaac
     6781 gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg cgcataacaa
     6841 taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg cttgttgcac
     6901 ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcacctcgg cacaagcgat
     6961 cagcgccaga acgctaatga tggcaaatgg tgtggtatta aatgtaacgc gggctgcccg
     7021 cagaattgcg gcagcaagta ttggaccgct caacaatacc gtcaggaaga aagccagcgt
     7081 agtgtaaccg ttatgccagg ttggcacggt gtcgatttga tacacgcagg tcatcatcca
     7141 gacgaaaatg acgccgagcg ccatgctgac aagtaaccag agtttcccca acgcttgcgg
     7201 cattttaccg atgaccgcca ccagccacca tagtccgcca acggcaaaaa atatggaacc
     7261 ggcagcaatc tcgttactca gaccagaagc tccgattcga ttcagcgagt taaacgctcg
     7321 cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca acagccagag
     7381 gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg cgaaccagcc
     7441 aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa agataaccag
     7501 tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc agataaccag
     7561 tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg ggttttgtga
     7621 aatgcgcgcg cggcagtggc gcgacggcag ccagagtacc gtgcttctgg cgcagctctt
     7681 caattggacc gaactcaagt gcacgcagcg ggcaggattc gacgcatatg ggttgtttcc
     7741 cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt tcagcattgt
     7801 actgtggtgc gccgtacgga caagccatgt ggcagtagcg gcagccgata caaacatctt
     7861 catcaaccac cacaaagcca tcttcacgct tatgcattgc gccgctgggg cagacttttg
     7921 tacatgcggg gtcatcgcaa tggttacagg agatggagag ataataagcg aacacgttct
     7981 ggtgccagat gccattatct tcctgccagt cgccgccagc atattcataa atacggcgga
     8041 aactgacttc cgggccaaga tctttaaaat ctttgcacgc cagttcgcag gttttacagc
     8101 cagtacagcg gctggaatcg ataaaaaatc catattgtgt ggtcatgggc tactccttaa
     8161 accttttcga tctggacaag attgctgtgc gacgggtttc cctttgctag cggtgaaggg
     8221 cggtgagagg tcagaatatt gatactgccg ccgtgatcga cccggtcacc aaacatatcc
     8281 gctttaagcc acgcaccttg cccgatggcg gtaacgccag gcagaatacg cggagtcact
     8341 tttgcggcaa tcagcatttc tccattattg ttaaataccc gcacggtatc gccatgacgg
     8401 ataccgcgtg cctgagcatc aatggggttg atccacacct cttgtgggca ggcctgctgt
     8461 aacacatcaa tattgccgta gctggagtgg gtacgcgctt tgtagtggaa gcccgttaac
     8521 tgcagtggat aggttttccg caggggagcg tcccagccat caaaacctga ggtatacgca
     8581 ggaatgggat gaataatttc atcttttttc aattcccagg tatctgcaat cttcgccagt
     8641 cgttcagaat aaatttcgat tttccccgaa ggtgttttca acgggtttgc ctgtggatct
     8701 tcacggaatg cgcggaaagc gacgtagtgt tcttccgggc attttttctt aaagatcccg
     8761 gtcgttttca tctcctcgta gtcgggcatc tcagggttac gttccttcgt tttcgcatgg
     8821 agatatttga tccattcatg ctgactgcga ccttcagtaa aggtttgata aacgtctggt
     8881 cctaagcgtt tggcgacttc actcagcatc cagtagatgg gtttgcgttc aaattttgct
     8941 gaggttgcgg gttgggcgag gatcacatag cccatattcc ctgcagattc atgagagata
     9001 aggtcttctt gctctgttgg catcaggtca ggcaacagga tatcgcaata cttagccgag
     9061 gccgtcatga agtggtcaat gccaacaatc atctcgcact tgctgtcatc ctgaagcacc
     9121 tcatgggtgt gattgatgtc gccatgttga ttgatcaatg tgttactggc gtagcaccat
     9181 aaaaacttga tggggacatc cagtttttct tttccacgaa caccatcacg ggtcgcggtc
     9241 attttcgtac catggtcgat ggcatctgtc catgtaaaaa cggaaatctg cgttttaaca
     9301 ggattctcga gcatcgggaa ccattctacc cccagatccc agctaccttt gcgtacacct
     9361 gagttgccgc cgtttatgcc gacgttaccg gtaagaacgg aaagcatggc aatagcgcgg
     9421 gacgtttgtt cgccgttgga gtgtcgttgt ggcccccaac cctgacaaat ataagcaggt
     9481 tttgctgaac cgatctctcg tgccaactgg ataatttttt ctgccgggat gctggtgatt
     9541 tttgctgccc attccggcgt tttagctatg ccgtcaggcc cttcgcccag aatataggct
     9601 ttataatgcg cgttacgtgg tgcgttggcg ggcagcgttt tttcatcgta accaacacaa
     9661 tatttgtcga gaaatggctg atcgaccatg ttttcagtaa tcagtaccca ggcaatcgca
     9721 caggccagtg cgccatcggt gccagggcga atgggcagcc attcatcttc acgccctgca
     9781 gctgtgtcgt tatagcgtgg atcgatgacg atcatgcgtg cgtttgaacg ttcgagggct
     9841 tgctcgacgt agtaagtgac accaccgccg ctcatccgcg tttctgccgg gttatttccg
     9901 aacataacga ccagtttcgt attggcgata tcatccgggc tgttgccatc attggcaccg
     9961 aacatataac tcattgcggc actgatctgt gcggtactgt agctgccata gcgactgaga
    10021 aaacccccgc aagagttcat cagacggtac gggacgttcg agttggtgat atttccgcca
    10081 tctacgcctg ttccgtacag gacatgtaca gcctcattgc cgtaatcttt caggatccgc
    10141 cgaagattat cactgatggt atccagggct tcgtcccaac ttatccgttc aaatttacct
    10201 tcaccgcgct cgccgacgcg cttcatggga tatttcaacc tgtcaggatg attcatccgt
    10261 cggcggatag agcgcccgcg taaacatgcc cgaacctgat gattaccgta aacgtcgtca
    10321 cctgtcgtat cagactccac ccagtacacg gtgtcatctt tcacatgcaa acgtagcaga
    10381 cagcggctcc cgcagttaac ggtgcaggaa ctccagaccg ttttctcttc tacccatctt
    10441 tttccagttc ccaggtacgg gcaatttccg ccagcttgct ggaataaatt tcaatcttac
    10501 cggaaggcgt tttaagtgga tttgcctcgg ggtcgtcacg aaatgctttg taggcgacaa
    10561 aatggccatt gggatcttta cgcttataga tacccatttt tttcagttcg tcgtaagacg
    10621 gtaacgccgg atctttggca agcattttgg cgtacagatg ttgtaaccat tgttcctgcg
    10681 tgcgaccttc tgtgaacttt tgatagacgt caggtccaag acgtttcgcg acttcactca
    10741 ggatccagta aatcggttta cgttcgaatt tttcgctggt gactggctgg aggaaaatga
    10801 gatatcccat gttaccggcg tagtcgttag gaataatatc ttcctgctca acggtcatca
    10861 ggtctgccag cagaatgtcg gcatattttg ccgtatgagg tcataaagtt ttcgatgacc
    10921 acaatcattt cgcatttcga ttcgtcctgc agaatttcat gcgttttgtt gatgtcagaa
    10981 tgctgattaa cgagggtatt tcccgcgtag ttccagatgg actccactgt cagacggatg
    11041 gatggacttg aagtagttga ctcaacgcta cttaaggtcc tgatatgact gacattaacc
    11101 atgataatac tgtatatgca tccagttctt tctctgggtc tccctatctg tatcagcaag
    11161 tctacacctc aagtcctgac acaaggctga cacaaatcaa ggaccttgca gtcccgcact
    11221 tgctcgcaag gctcgctacg ccacagttag tccgtagagc taacggtcga tatactatcc
    11281 gcttcagact caagggacag accacaccgt ttctttcagt atcgacccgc agcacagaca
    11341 ggagagtagc cacaatgcgt cagagagagc ttgcagccac cgctaaggcc ttcatgttgg
    11401 ataggcctga agtctcacta caggagctta cagagcatct ccggtctatg gctgagcagt
    11461 tcctgacaga cgctagtgac gattactgga atggacttga ggtcgctact ttggtggacg
    11521 agaagtccaa cctgagggag ctagccgcta cgcaagcctt gagtctggac cagcagaagg
    11581 gcataaggtt agcgcttgaa gtcctgaccg cagcacagca gagggtagac actggtgata
    11641 cctctggtct gattaagctg attgatgata ataaccatac tgattactca actataggcg
    11701 actctacgtc aattctgaag aatgagcagg gggatagcca gcagttttca cacaggagcg
    11761 ccaaccatct gtagtttttt caagcctagt gtcctcgctg ctggctgaga aggcccagac
    11821 cctgaagtca tccagctaca aggacctgtc gtcctcactg aataccgtct ctcggttctt
    11881 acctgaagac atggacctga tgtcccgctc tgagtggctg gctgtcaggg acgctatgct
    11941 ggcttctgag gtgagaccgt cgacgattaa caagctgctg actaaggcga agatgtgtct
    12001 tgattacggt ctgatgaatg ggcaacttga gggccgcaac cctattgaac ggatgaagct
    12061 gaccaaggat atcgactcca agcgcagagc gttcacagac gaggagctgg agagacttct
    12121 ggtccgtgtt gaggctgagt atcagttcac acgacacacg gctcacacga cctctgaggc
    12181 ccgtagatgg gcgtctctgg tttctgtagt tactggcgct cggtctgctg aggtctgcca
    12241 cctgactaaa cgtgacattg taaccttaga caatggactg gtctgtatcg atataaacga
    12301 agatggggac ggtaagtctg tgaagaacaa gcattcagtc cgacttgtgc cgctgaccga
    12361 tggcgttcat gggttcgacc ttgagtcttt cctcaaatgg gtagacatgc agcctgatga
    12421 tggtcctctc ttcgggatga ctccgagcgc ctactctagc tggtttaact caagggtcct
    12481 gactgaggcc ctaggagact ctcaggatgt ctctctccac agtctgagac attggctggc
    12541 gacccgaatg aaagagcgtg gagtcaatct ggtggacgct caaggcatct taggtcacag
    12601 cagtcagtca attacgtatg acctgtacgg gaaaggacac gctgtgggta ggctggctga
    12661 tgtgcttaag acagctttac tttaagtagg gagacccaag gtggagacca tatacggctt
    12721 gatggttgag accttgggtc actgaagtct gactgaagtt agggagggtg ataataatca
    12781 taatccctcc tgactatagg cccactggtc cgccttcggc tgtccgactc taagtctgtc
    12841 tgtggtctta tttaaactgc gtcctaaact ccagtccctc caaattccta tggtctcctc
    12901 ttgacattcc tgtttaactg tgagtcgcct ctggtctctc tttatatata cttccttaat
    12961 tcctgaggcg tctttagtcc ttgacatttg gttcaacctg tgagtcacgt ccggtccccc
    13021 tctttatatt tacttcctta attcctaggg tcgccttaat ccgtgacatc cagtccagcc
    13081 tgtgagtcac atcctgtcgc gctactgtat gcatcaccag tgatggactt gattatcact
    13141 ataggggaac caactaattc aactacagga gatactctat ggcacgacca atcgcaactc
    13201 acgacaacac gttcaccaaa gcctacttgc agcagcactg cggggacctg ctgtcattcg
    13261 atggtcaggg cgacctgagt ggttggctgg atgatgtcct tactggtgct ggtcgcctga
    13321 acgaatcaat ggcaagcaat accaagcctg tcagcccata cttgattctg acccaactac
    13381 taacgcacga cacattaact gtaagcgctg tgcaggagtc cctgagccgt aagcgtgttg
    13441 ccttgggtga acctatggtt tcaactcgct acgctcgcta cgtctatgct accgtcgtca
    13501 gcgccagtaa atccgtccag taccacgcca gtaaagctgg ttcatgatta cggaccctaa
    13561 actatcacta tagggagacc agcctccgcc tctgagtctg atgcgtcaga acatgcgagc
    13621 atctggtcaa ccgacattaa gtatctctcc taagtaatca aggcatgtga ccgcctctgt
    13681 cagacctcgt gtatcgacct ccagctcgtt agcgttcttc agtcatctca gttagtagct
    13741 cgtctactca ctacattggc ttaagt
//
LOCUS       DAJVQH010000108        13716 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJVQH010000108 DAJVQH010000000
VERSION     DAJVQH010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.108"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..444
                     /locus_tag="OQM87_003170"
                     /pseudo
     CDS             1..444
                     /locus_tag="OQM87_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            504..611
                     /locus_tag="OQM87_003171"
     CDS             504..611
                     /locus_tag="OQM87_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCS1509212.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(592..1323)
                     /gene="yehW"
                     /locus_tag="OQM87_003172"
     CDS             complement(592..1323)
                     /gene="yehW"
                     /locus_tag="OQM87_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCS1509213.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1328..2254)
                     /gene="yehX"
                     /locus_tag="OQM87_003173"
                     /pseudo
     CDS             complement(1328..2254)
                     /gene="yehX"
                     /locus_tag="OQM87_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(2247..3405)
                     /gene="yehY"
                     /locus_tag="OQM87_003174"
                     /pseudo
     CDS             complement(2247..3405)
                     /gene="yehY"
                     /locus_tag="OQM87_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(3412..4329)
                     /gene="osmF"
                     /locus_tag="OQM87_003175"
                     /pseudo
     CDS             complement(3412..4329)
                     /gene="osmF"
                     /locus_tag="OQM87_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
     gene            complement(4581..6878)
                     /gene="bglX"
                     /locus_tag="OQM87_003176"
     CDS             complement(4581..6878)
                     /gene="bglX"
                     /locus_tag="OQM87_003176"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCS1509214.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7074..8789
                     /gene="dld"
                     /locus_tag="OQM87_003177"
     CDS             7074..8789
                     /gene="dld"
                     /locus_tag="OQM87_003177"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1509215.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8827..9759)
                     /gene="pbpG"
                     /locus_tag="OQM87_003178"
     CDS             complement(8827..9759)
                     /gene="pbpG"
                     /locus_tag="OQM87_003178"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCS1509216.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9933..10520)
                     /locus_tag="OQM87_003179"
     CDS             complement(9933..10520)
                     /locus_tag="OQM87_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCS1509217.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10690..11268
                     /locus_tag="OQM87_003180"
     CDS             10690..11268
                     /locus_tag="OQM87_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1509218.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11385..12146)
                     /locus_tag="OQM87_003181"
                     /pseudo
     CDS             complement(11385..12146)
                     /locus_tag="OQM87_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12199..13635)
                     /gene="mdtQ"
                     /locus_tag="OQM87_003182"
     CDS             complement(12199..13635)
                     /gene="mdtQ"
                     /locus_tag="OQM87_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCS1509219.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGMETDINASKTRQQLNDVVGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
BASE COUNT         3245 a         3857 c         3476 g         3138 t
ORIGIN      
        1 atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag acactcacca
       61 cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact ctccaggcgc
      121 tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg gcttcggcac
      181 gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat accacccgtc
      241 tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc ctcgctcgcc
      301 cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct ggtgtggtgc
      361 ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga acaaggccat
      421 gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact gtacaatcct
      481 ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc gccctgttcc
      541 tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc tttattttgc
      601 gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga tcgccgccag
      661 tgccaccagt aacgccccct ggatcacata cgcggtatta aatccgctaa gcccgatgat
      721 gatgggcgta cccagcgtac tggcccctac cgttgaggcg atcgtcgccg taccaatgtt
      781 gataatcacc gaagttcgca cgcccgccag aatcaccgga gccgccagcg gtagctcgac
      841 cttacgcagt cgctgacctc gactcattcc catacctttc gcaacttctg tcacgctggg
      901 atcaatcgct cccagcccgg caagtgtcac ctgcaggatg ggcagcacac cgtaaaggat
      961 caaggcgata atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa tcgccagcac
     1021 tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg ggcgaaattc
     1081 cgcgccccac gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca ccgcaaacaa
     1141 actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac tttcttgctg
     1201 ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc tgtaaggcag
     1261 ccagaaaatc agcgccacaa aaagagcaat gagccagaac agcggatcgc gcaacatctt
     1321 catacgctta cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa ggctggccct
     1381 gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagggag agcgcatcgc
     1441 gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga cgcacgtaat
     1501 ccgccacgct acgtaacgaa agcaggtgca cccccagttc actacgtcca aaaaactggc
     1561 ggacaaaatc attagccgga cgagtcagca tcgtcagcgg attcccctgc tgcaccactt
     1621 caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca atatcatgag
     1681 tgaccagcac aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct tgttgcaacg
     1741 cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc catcagtaag acttgcggat
     1801 cggcagccag tgcacgcgcc actcccacac gttgctactg accaccggaa agctgatgcg
     1861 gataacgctc acgcaaattt gactccagcc ccagtagcgc cattaattcg tcgatacgat
     1921 cgtcaatccg cgcccgcgac catttttgta attgcggcac ggtagcgatg ttttgcgcca
     1981 cgctccagtg ggggaacagg ccaatagatt gaatggcata gcccatccgg cggcgcaact
     2041 ccagtactgg cagcgagcga atttcttctc cggcaaagcg gatcactccg ctgtcatgct
     2101 ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc ggagccagat gtgccaatca
     2161 gcaccgaaaa actcccttcc tgaaaattga gattgagatc gttaacggct ttttgtgcgc
     2221 cgaacagttt gctgacatgg ctaaattcaa tcattacgtt tcaccttcag cagtgcgata
     2281 agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa ttaccgggat cacccccagc
     2341 aacactaaat caatggcgct gcttagcagc ccctggaaaa ccagcgcacc aaaaccgcct
     2401 gcgccgatta acgccgcaat caccgccata cctacagttt gcaccatcac cacccgcagg
     2461 ctgcgcagaa ataccggtaa cgccagcggt aactgaacat gcaggaaacg ctgcgccccg
     2521 ctcatcccca tcgctctggc gctctccagc acatcgcgcg ggatctggtt caaaccgact
     2581 accacgccgc gcaccagcgg cagcaaggca tagagcacca gcgcaatcag tgcgggtgtc
     2641 attccggttc ctgctatgcc gagcttcccc agccacggaa aggccgtaac cagcgcggca
     2701 agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca cggtctgaat gacattgagc
     2761 agagaaaaaa ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc caacggcaca
     2821 ccaatcacta acgcaggcag caccgcacca aacagcaacg tcagatgttg tgccagcgcg
     2881 tcgtcaaaca catcctgacg gttggcgtat tctttcattt agtgagagat cgttaagcgt
     2941 gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca accagcgcca
     3001 cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg ccagcgccgc
     3061 cgccagccag aaaccactgc cgaggctggt acgcgccagc gcactgccat tttgcgccag
     3121 ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt gcgccagaat
     3181 gagtgcaaaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc aggcgcaacc
     3241 aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgaa gcccttcacc
     3301 cgagactaaa cgattaggcg cgtaactgat aaacggcagc gccgctgcga ttgccgtcag
     3361 caacaacagc agcgccagaa caggattaat acgtagataa gtcacgggaa attacttcgc
     3421 ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc cttccactgc
     3481 aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga ctggctgtag
     3541 ccactgtgcc atttgcggat actccttcaa caccgattca cgcaccactg gtgcaggcgc
     3601 gtagataggt tgcacgcctt gcggatcgct taaggtttgc agccccagcg ccgcgaccgg
     3661 agcgtcagtg ccgtaagcca ttgcggcatt aacgccagag gtttgctggg cagcggcttt
     3721 aatcgtcacc gccgtgtcgc caccagccag tgacagcaac tgatcctgac cgagcttaaa
     3781 gccataggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact ctgccgaggc
     3841 tgccagtttg aaggtgccgc cctcttgcag ataacgactc aggtcggcaa gcgaagtgag
     3901 tttgtttttc tctgccacat cctgacgcac ggcgatagtc caggtgttat ttgcaggcgc
     3961 gggcgtcagc cagattaact tgttgtgctc cgcatcgagc tttttgactt tctcgtagcc
     4021 ctgcccggca tttttccacg ctgcatcgtt ttcatcttta aagaagaaag cgccattgcc
     4081 ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca ccacaggagt
     4141 cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg ctttcgagta cctgcaagat
     4201 gatgttgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa cgggggaagc
     4261 cgcctatagc agcaggctca cggctgccaa cataaccagt gaacctgccc agagctttaa
     4321 gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta agagaaaagc
     4381 atagttgata atgacagagt tagcctgagc ggaaggattc agttgcagaa tcagataaat
     4441 acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc gccttatccg
     4501 gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa gggcgcaaac
     4561 gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac gcgtgcggaa
     4621 tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat ctgttgattc
     4681 cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc gcccggtttc
     4741 agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga agccgtcaca
     4801 tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt cgtcacctgc
     4861 acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt cacatcagag
     4921 acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc gttagcttca
     4981 tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg accggtgttc
     5041 agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat tggcagcttg
     5101 ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc ttcagtcccc
     5161 gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag cgccagcgga
     5221 cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc cgcaatcaag
     5281 tcacgttggc cttgcggaat agtgatatcg gtccggctgg aggcttcgtg cgccatcccc
     5341 tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg caccgcttca
     5401 tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata ctgattcagg
     5461 aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag cactttaccg
     5521 ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc ggcaacaccg
     5581 gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc aaccaccgca
     5641 atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa cactaggctt
     5701 tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt ggtatccacc
     5761 gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaaccc catatcatat
     5821 ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatggtcac tttgccggat
     5881 ttaatcaacc caggcagata cttcgagtag tactcgtcgc tcatactcat gttgattccg
     5941 gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg tttaatcagc
     6001 tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg gtcgcgcaga
     6061 acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga gttcagcgcc
     6121 accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat ataatcatta
     6181 aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc taccgcgccg
     6241 tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg gcttttaccc
     6301 tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc ttcgccaaaa
     6361 ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg tgcccaggtc
     6421 atattcaggc catcatctgc cgcttcataa gcagagacac gcccgaccgt tttcaccgca
     6481 tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg ctgaccgtgc
     6541 agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat cacctgatcc
     6601 tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac ctgaccgtct
     6661 ttgatcatct cgcggatcgc ctctttcgga ttatccgggc ctacgctgat taagcgcagc
     6721 tgaccaattt tctcatcaac catcattttc ttgagcagat cggtgacgaa cgcatcccgc
     6781 gcttcaggcg ttaatggatg gttaccgaat aaatcatccg ccagtgctgg ctgcaggacc
     6841 agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct tcctttgttg
     6901 cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat gatggagaga
     6961 gaaaaagaat actcagttta ttgtctgaat tttcaaaata ttcactcgct gaattgtcat
     7021 acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg aaaatgtctt
     7081 ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg gtcggtcatt
     7141 cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc cgttctggtc
     7201 agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg cgggtgctga
     7261 aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca ggcctgaccg
     7321 aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc agcaccctgc
     7381 gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat ccgggcacca
     7441 cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcat tcggtgattg
     7501 gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc ggcggctcgc
     7561 tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata gatgaagatg
     7621 gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg gagcagatcc
     7681 ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat ggtcgtcacg
     7741 cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg cccgcacgtt
     7801 ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag ctggccgtct
     7861 ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt tatatcggca
     7921 ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac ttcgaaaatc
     7981 tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa aaatacggca
     8041 aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc ttctttaatc
     8101 tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg cattttactg
     8161 accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg cgcatgaaaa
     8221 actggcgcga taaatacgag catcatctgc tgttaaaaat ggcaggcgat ggcgtcggtg
     8281 aagccaaatc gtggctggtg gattatttca aacaggccga gggcgatttc tttgtctgta
     8341 cgccggagga aggtagcaaa gcgtttttac accgtttcgc cgctgcgggc gcagcaattc
     8401 gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcactggat atcgctctgc
     8461 ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc cagctggtgc
     8521 acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac atagtgaaaa
     8581 aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag cagcgcggcg
     8641 cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag acgttgcaga
     8701 agttctatcg cgagaacgat ccgaccaata gcatgaatcc ggggatcggt aaaaccagta
     8761 aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat cgccatccga
     8821 attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg ctttttgttt
     8881 tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa tccaggtacg
     8941 caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca ctaccagcgc
     9001 caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg cattggtaaa
     9061 gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat tagtattgcg
     9121 gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg tagtacttaa
     9181 ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt cacgggcagt
     9241 tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt tcattccgag
     9301 cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg gataatggtg
     9361 cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca gcatatcttt
     9421 acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct ccggcgtctg
     9481 gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca caaccatcgc
     9541 ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt tcgaatagat
     9601 cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt ccggttgtga
     9661 agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gcaaaaggca cagccagcat
     9721 cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc agatagtggt
     9781 tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc gacgccatca
     9841 taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag taaatgctgc attgctgaca
     9901 tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc gtagccccat
     9961 agtataacgg ttagggtgag cagcacttcc agtaccagca cgccaatcgc cagcgtcgaa
    10021 ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat accaaggtaa
    10081 agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa ccagaccagt
    10141 ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc cgcgaagacc
    10201 atgcagtgcg caagagacgg acgctgcggg taattgcgcg ccatccacca gatgacccgc
    10261 cccatcaccg caaccccagc cagcataacg ccgtaaaaca agacagccag cgccagtccg
    10321 gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat ctgtgtagtg
    10381 ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg ggtgtaatgg
    10441 tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg atgggaaaac
    10501 agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct gacagttcaa
    10561 gtataagcca gcttgtgatt attttttgtt catccatgta atttgcaccg tctgatgtat
    10621 gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca aagggagaca
    10681 gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc gctggttatc
    10741 ggtagcctag cggaaggtga aaccgtgact ttgctgggag gcgttgcggc acatcagggg
    10801 ctattaaagt tcccactggt ggtactttct gtggcgcttg gtggcatgat tggcgaccag
    10861 gtactctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt ctcgaaacat
    10921 caggataaaa ttgagcgggc gcagaaactt atccaacgcc atccgtatct gtttgtcatt
    10981 ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg tgccagccag
    11041 ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg ggcgttgatt
    11101 tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca caatctcgac
    11161 cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt gggcgtgcgc
    11221 tggtggctga aacggcgcgg gaagaaaaag ccggataatc aggcgtaaag cagttgccac
    11281 agataagcat tcactccaca tccggcaccg ggtgttccat gcctgatgcg acgctggtgt
    11341 cttatctggc ctaacccatt gaaatatcca cgcacctcac tgcgctattc tgggttgaac
    11401 tgcggattcg ccaacataaa gccgccatcc actatcaacg actgccctgt cgtgtaattt
    11461 gcgccctccg aacaaagcca caccaccagg ctggcaatct catgcgttgc gccaaaacgc
    11521 cgtagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tatcatccat gctgttcatt
    11581 ggcgtggcta tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct gaccaactcc
    11641 agcgccatcg ttttggttaa cccgccgagc gcatgtttag cggctgtgta ggcgctggca
    11701 tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc gccctgccct
    11761 tatttcacca tctgacgagc cgcaatttgc gagcataaaa atgcaccatc aacatcaacg
    11821 gtaaaaatct tgcgccactc atcaaaagcc atatcaagaa acggcgcttt ggtcattgca
    11881 cccgcattat tcaccagcac atcaatacgc ccaaatcgct ggatgagtgt ctccagcgcc
    11941 tgcgcacctt ctggcagatt gccgagatcc agttgcacga tctccgcacg tacgccgtgg
    12001 gtaaccacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca ggtaatgcca
    12061 atatcaaacc cctgatgcgc cagtaataac gcgcactctt tgccgatccc cgaatcggaa
    12121 gcggtaatta tcgcgaccta tgccatcgag ttctccactt aacgctgaat aaacgttaag
    12181 tatagaaggc gcatatcatc agcgtttgta acccccgccc agcgcaccgg tgagtcgaat
    12241 ggaggcatcc agccactgcc cttgcaataa caggccattg gcccgctcac gcagcgccgg
    12301 gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc gggcctgcgc
    12361 aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt ttttctccgc
    12421 cagtgtctga acctgactgg ctgcccgcgc cacgtcattc actgcttcaa ccaccgcttt
    12481 gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcgagat tggcgttaag
    12541 acgaccacta tcgaaaatag gtagcgtcag gcctgccgta acgcccattt gctgcgcgga
    12601 atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca tcaggttgat
    12661 gtcaggataa aatgccgctt ttgccgcatc aatagtgctt aacgatgact caacgtacca
    12721 gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag taccccagtt catcaggaag
    12781 ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgacttag tctggtgatt
    12841 tgtgagtgca tttaaccgtg cctcaataat tttcattttc cccacgacat cgttgagctg
    12901 ctgccgggtt ttgctggcat taatatcggt ttccataccg tcaactgaag aagtaatccc
    12961 gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt ctatttgctg
    13021 caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg ctacgctgcc
    13081 agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg tacccaggcg
    13141 ggcagtaacc tccgcccgat tctttcccca gatatcgaga tgccagcccg ccgttaagcc
    13201 aaaagttccg ttggtgtacc acggaccggt cgtacctgcg gccggatcgt tcagagcaaa
    13261 cggccccatt agcccttctg ccgacatttt ttgccgctcc atatccgccg aaaagtcgat
    13321 ctgcggacca tcctgagtgg caactgcctt cgcctgggct tcagctagct gaatgcgctg
    13381 ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaatg aagtgagctg
    13441 attatcgtga tactccagcc accattggct gtctggccaa ccatttttca gcgccgtggg
    13501 taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc gggtttcatg
    13561 cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg ctggataaaa
    13621 ggaatcacga ttcatggggg aataatcagg taagaaacgg tgcgcggaga ttaccgtgtg
    13681 ttgcgatata ttttttagtt tcgcgtggca atacat
//
LOCUS       DAJVQH010000109        13562 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJVQH010000109 DAJVQH010000000
VERSION     DAJVQH010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.109"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            296..1549
                     /gene="kch"
                     /locus_tag="OQM87_003183"
     CDS             296..1549
                     /gene="kch"
                     /locus_tag="OQM87_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCS1509220.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1604..1777)
                     /locus_tag="OQM87_003184"
     CDS             complement(1604..1777)
                     /locus_tag="OQM87_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCS1509221.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1920..3380
                     /gene="cls"
                     /locus_tag="OQM87_003185"
     CDS             1920..3380
                     /gene="cls"
                     /locus_tag="OQM87_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCS1509222.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3415..3744
                     /locus_tag="OQM87_003186"
     CDS             3415..3744
                     /locus_tag="OQM87_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCS1509223.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="OQM87_003187"
     CDS             complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="OQM87_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCS1509224.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="OQM87_003188"
     CDS             complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="OQM87_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCS1509225.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGAATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="OQM87_003189"
     CDS             complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="OQM87_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCS1509226.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="OQM87_003190"
     CDS             complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="OQM87_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCS1509227.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="OQM87_003191"
     CDS             complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="OQM87_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCS1509228.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="OQM87_003192"
     CDS             complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="OQM87_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCS1509229.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11245..>13562
                     /gene="adhE"
                     /locus_tag="OQM87_003193"
                     /gene_synonym="adhC"
     CDS             11245..>13562
                     /gene="adhE"
                     /locus_tag="OQM87_003193"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCS1509230.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARR"
BASE COUNT         3548 a         3357 c         3143 g         3514 t
ORIGIN      
        1 agatccaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc tttccttata
       61 gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt tctttatatg
      121 atgtgagcat gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt
      181 tgattactgc ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt
      241 tcagaaaatg tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag
      301 tcactgggct acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat
      361 tctcgcgctg gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc
      421 ggtcaatttg cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct
      481 ggcaaacgcc ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact
      541 gttccgggca aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta
      601 caccctgcat ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt
      661 gtttttgctg atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt
      721 tgcatttatt agtttcacga cattactgtt ttactcaacc tacggtgcac tttatttaag
      781 cgaaggtttt aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac
      841 catgtcaacc gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac
      901 tatttcggtc attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg
      961 tccgcttatc cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg
     1021 taaagatcat tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact
     1081 gaatcaacgc ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca
     1141 acttgagcaa cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc
     1201 agtattaaag aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga
     1261 tgcagataac gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt
     1321 tctcgccgtc agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat
     1381 tctctcaccg caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat
     1441 taataatgat atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa
     1501 cgatgaacaa gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaacgac
     1561 ggaagagtaa acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg
     1621 caaacaataa cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca
     1681 tatttcggcg cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca
     1741 tgcgccagcg ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca
     1801 gaatggtcat tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg
     1861 ccagatgaat ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta
     1921 tgacaaccgt ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg
     1981 caggcgtaac tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc
     2041 tgttgattat ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc
     2101 tccatttagg caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat
     2161 ggcttaacga ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg
     2221 cgccattatt caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc
     2281 tacaactgat gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg
     2341 cgcgccataa tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg
     2401 tggctgaatc attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact
     2461 ccgccgggag tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta
     2521 ttgaagtggt cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc
     2581 tgcgccaaca tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga
     2641 atatggtcga tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga
     2701 tggcgcgtat ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg
     2761 agattgaaac cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg
     2821 aacaggccag tggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc
     2881 tcattcacca ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca
     2941 cgccctactt tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg
     3001 gggtggatgt cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca
     3061 gtcgcgcatt ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg
     3121 ggttactgca taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta
     3181 accttgatat gcgtagtctg tggctgaatt tcgagattac cctggcaatc gacgataaag
     3241 gttttggtgc agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg
     3301 atgcccgttt atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact
     3361 tcttcagccc gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat
     3421 atggatctaa acaatcgcct gactgaagat gaaacgctta agcaggccta cgatattttt
     3481 ctcgaactgg ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt
     3541 gaagagcgcg gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat
     3601 ttcgatttga acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat
     3661 ggtgaaatca acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt
     3721 tgccatatca tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg
     3781 tcagccctta ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac
     3841 tcccctccag aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac
     3901 ggaaaacaca accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga
     3961 tggttttgtt cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg
     4021 tgtaaggatg tagtggattg tggtagacct catcataggt ccccagttct actgcatggc
     4081 cgagatacat caccaacaca cgatcggaaa tgtgttttac cacggccagg tcatgagcaa
     4141 taaaaattaa tgacaatccc atctcacgtt gcagctgctg gagcaggttg accacctgcg
     4201 cctgaattga cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa
     4261 gaataagagc acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat
     4321 agcggttaat caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc
     4381 gaacttcctg gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc
     4441 cgatggtcat acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac
     4501 tgcgaacggc acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa
     4561 catgaccgtc ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc
     4621 atcccgattc ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat
     4681 cgacggcttt gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa
     4741 agtgcacttt aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca
     4801 taacagttcc tccaccggtt taaagcaagc acgcagacgg ccaggcgtaa actcttccag
     4861 cggcggtgcg ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc
     4921 tttcggtaat cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc
     4981 cgcatcaaga cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata
     5041 aaagacatcg cgcgcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt
     5101 gtcacagatc cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa
     5161 ttcccgcttc agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc
     5221 tgtagtgggt tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat
     5281 gactcgctga cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc
     5341 ttccggcatt tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct
     5401 catgttctta tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt
     5461 caacgaagtc attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag
     5521 ttcacgctct ggcaaattga ggatttcacg cccattgaag gttgccgctc cgccaatgcg
     5581 tccgttggca gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga
     5641 ctcacctaca atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc
     5701 cgtgacgtcg ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc
     5761 gtcagcctgt tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact
     5821 ccttaacgat ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa
     5881 aacagtgtca ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc
     5941 gagttcgcgc catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac
     6001 cccaggaagc taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag
     6061 accaccacca caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat
     6121 acaccgccaa cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca
     6181 cgcacaatac gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg
     6241 atgttttggc cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg
     6301 aggatttcca gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc
     6361 gaaccataaa gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt
     6421 gagatacgcc cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca
     6481 aagtagtgac cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca
     6541 taggcaaact gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact
     6601 atcagactgg caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag
     6661 ctgcgccctt cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta
     6721 cttaacatca tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata
     6781 gcacatcgac aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta
     6841 acactaagga atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca
     6901 aaccataaat ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca
     6961 tataggagag cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca
     7021 taggtaaccc tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag
     7081 agccacgggt aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca
     7141 atatcatgaa tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga
     7201 tcgcaaatat catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca
     7261 ttgccagccc catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc
     7321 cagcactaac acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa
     7381 cgggaaaact ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac
     7441 cgaaatcgcc atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat
     7501 taagatgata tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg
     7561 taaaagggct tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata
     7621 gcgtcggaat cgcttccaga cagcgacgta gaataaattt taacattgcc ctaccttctg
     7681 gcctgtgcct tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc
     7741 acgtattgcc attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga
     7801 tctttgccgg tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa
     7861 acaggaacaa tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg
     7921 cgctgcgcct cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc
     7981 ttataatgcg cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt
     8041 tcgttgtagt ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg
     8101 tcgaggaacg ttttccactc ctggttaacc agtttgacgt ttacaccaat gtttttcttc
     8161 cacaatgaag aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg
     8221 ttgatggtca acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct
     8281 tcgttacgtt tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca
     8341 gtatacggtg gagtgtaacc ataggcgggc atgtcgccct gcgctttcac tttattaaca
     8401 atgatatcgc ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc
     8461 ggtttctggt tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg
     8521 tccgggatct cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg
     8581 tcgatttcac cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag
     8641 gttacctggt taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg
     8701 attcgttcgt ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca
     8761 ggctgggtcc atttttcgcc gaatttctcg atagcggctt ttggcaccgg tgaagttgat
     8821 gggtgaacaa gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg
     8881 tgatcatcaa tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca
     8941 tcaataccgg cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga
     9001 tcaacagaac gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac
     9061 catttcgcat ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat
     9121 tcagcgacgc caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg
     9181 tctcggctga tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact
     9241 tctgaaccat tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca
     9301 tcagctgcca gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa
     9361 cttctcttgg tgatgttggt cattgttttt tggactccct cattataatt actggtatta
     9421 accagcatgt gtaatcccgg attgggaccc tgcacaggct cggagacttt ctgctgtcag
     9481 gttactttat cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat
     9541 caccttatgt cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc
     9601 tctttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
     9661 taccaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt
     9721 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
     9781 tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg
     9841 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg tagtggaatt
     9901 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
     9961 tgatggtgag gagaagcagt taaggaaatc cttataacta actgtaattt gagaagaata
    10021 tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga
    10081 agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
    10141 aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca
    10201 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca
    10261 tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
    10321 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
    10381 ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg
    10441 atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta
    10501 tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt
    10561 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
    10621 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
    10681 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
    10741 cgggaaaatc aaaaagggtc tgaatcacgg ttagctccga agcaaaagcc ggataatgtt
    10801 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
    10861 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
    10921 atgtagccac caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat
    10981 gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt
    11041 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
    11101 aactctttta gtaaatcaca gtgagtgtga gcgcgaataa gcttttgatt ttcatcggtt
    11161 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
    11221 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
    11281 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
    11341 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
    11401 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
    11461 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
    11521 gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg
    11581 accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac
    11641 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
    11701 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
    11761 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
    11821 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta
    11881 ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca
    11941 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
    12001 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat
    12061 ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg
    12121 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
    12181 ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc
    12241 gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg
    12301 gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
    12361 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct
    12421 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
    12481 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
    12541 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
    12601 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
    12661 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
    12721 aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc
    12781 ttcttcgaag tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
    12841 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg
    12901 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc
    12961 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg
    13021 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
    13081 gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg
    13141 attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
    13201 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
    13261 ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa
    13321 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc
    13381 gcatttgcga acgccttcct gggggtatgt cactcaatgg cgcacaaact gggttcccag
    13441 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat
    13501 gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
    13561 cg
//
LOCUS       DAJVQH010000110        13544 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJVQH010000110 DAJVQH010000000
VERSION     DAJVQH010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.110"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(1..892)
                     /locus_tag="OQM87_003194"
                     /pseudo
     CDS             complement(1..892)
                     /locus_tag="OQM87_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1133..1933
                     /locus_tag="OQM87_003195"
     CDS             1133..1933
                     /locus_tag="OQM87_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1509231.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2011..2781
                     /locus_tag="OQM87_003196"
     CDS             2011..2781
                     /locus_tag="OQM87_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1509232.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2829..4187)
                     /gene="mltD"
                     /locus_tag="OQM87_003197"
     CDS             complement(2829..4187)
                     /gene="mltD"
                     /locus_tag="OQM87_003197"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCS1509233.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4259..5014)
                     /gene="gloB"
                     /locus_tag="OQM87_003198"
     CDS             complement(4259..5014)
                     /gene="gloB"
                     /locus_tag="OQM87_003198"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCS1509234.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5048..5770
                     /locus_tag="OQM87_003199"
     CDS             5048..5770
                     /locus_tag="OQM87_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1509235.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5767..6234)
                     /gene="rnhA"
                     /locus_tag="OQM87_003200"
     CDS             complement(5767..6234)
                     /gene="rnhA"
                     /locus_tag="OQM87_003200"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCS1509236.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6299..7030
                     /gene="dnaQ"
                     /locus_tag="OQM87_003201"
     CDS             6299..7030
                     /gene="dnaQ"
                     /locus_tag="OQM87_003201"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCS1509237.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7163..7239
                     /locus_tag="OQM87_003202"
     tRNA            7163..7239
                     /locus_tag="OQM87_003202"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7197..7199,aa:Asp,seq:gtc)
     gene            complement(7370..8416)
                     /locus_tag="OQM87_003203"
     CDS             complement(7370..8416)
                     /locus_tag="OQM87_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCS1509238.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8427..9422)
                     /gene="tssG"
                     /locus_tag="OQM87_003204"
                     /pseudo
     CDS             complement(8427..9422)
                     /gene="tssG"
                     /locus_tag="OQM87_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9419..11302)
                     /gene="tssF"
                     /locus_tag="OQM87_003205"
     CDS             complement(9419..11302)
                     /gene="tssF"
                     /locus_tag="OQM87_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCS1509239.1"
                     /translation="MDPRLLGYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRPLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11318..11812)
                     /gene="tssE"
                     /locus_tag="OQM87_003206"
     CDS             complement(11318..11812)
                     /gene="tssE"
                     /locus_tag="OQM87_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCS1509240.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11809..12639)
                     /locus_tag="OQM87_003207"
     CDS             complement(11809..12639)
                     /locus_tag="OQM87_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HCS1509241.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12626..13540)
                     /gene="tagK"
                     /locus_tag="OQM87_003208"
                     /pseudo
     CDS             complement(12626..13540)
                     /gene="tagK"
                     /locus_tag="OQM87_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3102 a         3521 c         3617 g         3304 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattatcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aaaattgttc cgctgcccgg ctaaagctgc
      841 cgctttcgac gaccgaaaca aaaatggcga gttcttccga cgtggctttc atttttgctc
      901 ctgttgcaaa atagatgaga tattttgaat ttatttgtca ttaaaccatc aggatgcgtg
      961 atatgtcatc cgatttaatg ttctcaataa tgagcaaatt tctgaccggt gtaagcactt
     1021 gcttacataa caatatacaa ttgctcgttg aaagagtgag ctaaaatccc tataacagta
     1081 gaaccctccc gagtgcggaa gggttgacgt aatagaggtt tcaaagtcaa aagtgcgaaa
     1141 aaacacctat gccatgcgct atgttgccgg acaacctgcg gaaaggatct taccgccggg
     1201 gtcttttgcg agcatcggcc aggcattacc acctggagaa ccgttaagta ccgaagagcg
     1261 tattcggatc ctggtgtgga acatatacaa acagcaacgc gctgaatggt tgtcggtatt
     1321 aaagaactac ggcaaagatg cacatctggt gttattgcag gaagcgcaga caacgccaga
     1381 gttagtacag tttgcgaccg ctaactatct tgccgccgat caggtgcccg ctttcgtgct
     1441 gccacaacat ccttccggtg taatgaccct ttcggcggcg catccagtgt attgctgccc
     1501 gttacgtgaa cgagaaccca ttttgcgtct ggcgaagtcg gcactggtga cggtctatcc
     1561 attacctgac acccgcctgt tgatggtggt taatatacac gccgtcaact tcagtctggg
     1621 cgtggatgtc tatagtaagc agttacttcc tattggtgat cagatagctc accacagcgg
     1681 cccggtcatt atggcgggag atttcaatgc ctggagccgt agaaggatga acgcgttata
     1741 tcgctttgcg cgggaaatgt cgctgcgcca ggtgcgtttt accgatgatc agcgccgtcg
     1801 ggcgtttggc cgcccgctcg attttgtttt ctaccgtggt ctgaacgtca gcgaagcttc
     1861 tgtattggtt acgcgcgctt ccgatcacaa tccgctactc gttgaattca gtcccggcaa
     1921 gcctgataaa taaggtatgt caggtctgcc acagggcaga ccaacgtttg gcgctgtcct
     1981 tttatactca aacaacaaaa ggacagcgca atgacaacac aatcccacca tgaccacgta
     2041 gaaaagcagt ttagctctca agcctgcgaa tatctgacca gtaccgtaca tgcatccgga
     2101 cgagatttac agcgcctggc ggtgcgtctg gctgattatc ctggtgcaag tgtacttgat
     2161 atgggctgcg gagcagggca tgccagcttt gttgctgcgc aaaacgtgag cgcggtggtg
     2221 gcgtatgact tatctgccca aatgctggat gtcgtggcac aagctgccga agcccggcaa
     2281 ctgaaaaata tcaccacccg ccagggatat gctgaaagtc tgccatttgc cgataacgca
     2341 tttgatattg ttatcagccg ttattctgcc catcactggc atgatgttgg tgcagcactg
     2401 cgtgaagtga accggatatt gaaacctggc ggtaggatga ttgtgatgga cgtaatgtct
     2461 ccgggtcatc cagtgcgcga catctggtta cagacggtag aagcattacg cgatacctct
     2521 cacgtacgaa actacgccag cggtgagtgg ttgaggttaa tcaatgaagc caatctgata
     2581 gttgataatt taattacaga taagttaccg ctggaatttt gttcatgggt cgcgagaatg
     2641 cgtacgccag aagcgttagt agacgctatt cgcatttacc aacagagcgc atcgacagag
     2701 gtgagaacgt attttgcctt gcagaatgac ggctctttca ccagtgatat catcatggta
     2761 gaagcacata aagcggcata aataaaaaag gcaccggggg aatcggtacc tttttattat
     2821 ctggtttgtc aggaatctgg catgttgttg tttttcacaa acaacgtcag cttgtcgcct
     2881 ggttgcagat tcgcagtgtc gctgttccag cgcatcacat ctttgatgtt cacgccgtgg
     2941 cgtttagcaa tgcttgaaag cgaatcgcct ttgcgcacac gatacgtaat gctatcgctg
     3001 ttgtttgcca gtcgctgtgc gctactgcct gcaccaatcg tcaaactttg gcctggcttc
     3061 agcttagatc cgcgcagttt gttccactgc tgcaaatctt tggtgcttac gccgagacgt
     3121 gaagcgatgc ttgaaagcgt gtcgccagag cgtacggtgt aaacacggct gttaagcggc
     3181 gtattgtcgg caaccagcgt cgactgtaca gcagcaattt cgcctgaagc gagagattca
     3241 cgcagttgat ctgcatgctt ctttggcacc atcacgtact gcggcccact tgcgcccagc
     3301 gtggagcctt tcacgccagc gttgaatgtc ttcagcttgc tgacggaaat ccccgccata
     3361 tctgcaacct tcgccatttc aaccgggcta ctcaggtgca cacgcgccag agcacggctt
     3421 tcatcggtcg ttggcagacg tacgccataa cgcttgctgt ttttgagaat atcactcaat
     3481 gccagcattt taggcacgta ctgcttcgtt tcctgcggca acggtaacga ccagaagtcc
     3541 gtggatttcc cacgcgcttt gttcgtttta attgccttca tgacccgacc ttcgccgctg
     3601 ttataagccg ctacggtcag aagccagtcg ccgtcaaaca ttttgttcag acgctgcatc
     3661 atattcagcg cggcagttgt tgaagcaaca acatcgcgac gcgcgtcata attgcgggtc
     3721 tgtttcaaac cataattgcg ccccgtgctc ggaatgatct gccagatgcc tgcggcattg
     3781 gcgccagacg ttgcgtgagg atcaaaagcg ctctccacta tgggtagtag taccagttcc
     3841 ataggcatgt tacgtttttt aacttgccct gctatccagt acatatacgg ctctgcccgt
     3901 aaagttacat cgtggagata gctcttattg cgtaaatatt tctgtttctg ttcgcgaatc
     3961 cggtcatttt ccggaattcc catctttagc tcgtcgccaa tgaaagccca caagtcacca
     4021 tctggcgcga tagacgtccc atcgtccatc catcgtgcct gacttgtaaa ctttgctgct
     4081 tccccttgac cagctgcaga aaggctctgt gcgtgctgtt gaacgttgcc ggtactctgg
     4141 caacccacga gcaggacaga ggcgagtaat atcgcttttg ccttcatgtg tgtgtcaata
     4201 gttgcttaaa agacgaccga tcataacggc gaacggagcc gatgacaaga aagttttatc
     4261 agaacctatc tttctttgac cttaaccatg caaaacgctc ttcaggttgt tgcaataatg
     4321 tttcttcatt aattacatta attaaatcaa tatcttccgt tcttaaaaaa acattaattt
     4381 gccgctcatt tttcagaatt acgggtagtg ttatttgatt ttttgcccgt aactccttaa
     4441 ctttacgata ataatcattt atggacaaat cgtgcggaaa aatactcaaa gcaaacttca
     4501 tatttgataa cgtatattca tgagcacaac ataccaatgt atcgtcaggt aacgcactta
     4561 gctttttaag tgattgatac atttgtaaag ctgtcccttc aaacaaccga ccacacccac
     4621 cagaaaacag cgtgtcgccg caaaatagat aaggtttact gaagtaacag atatgtccta
     4681 aagtgtgacc cggtgtggta attacactaa attcatgccc taaaacgaag gcagtttcgc
     4741 catctttgac tacctgtgtt gttcccttat cttgtgtctc ttgtggacca tacaccacaa
     4801 tttgcggaaa cttttccacc agttctttta cgccgccaac gtgatcgtgg tgatggtggg
     4861 ttagaaatat ggcctccggt tgccagttat tggcggtaat ggcgtttaat actggctctg
     4921 cgtctccggg atcgacaatc aggcagcgac ctgcctcatc attcaaaacc cagatgtaat
     4981 tgtcatcaaa ggcgggaata ctgttaagat tcatagatta cctctcagtg taaaacggaa
     5041 ggttgtgatg aaaccggcaa gagtccctca aactgtcgtg gctcctgatt gctggggcga
     5101 tttgccctgg ggagagcttt atcgcaaggc gctggagcgc cagctcaacc cgtggttcac
     5161 taaaatgtat ggttttcatc tgcttaagat tggcaattta agcgcagaaa tcaattgcga
     5221 agcgtgcgcg gtttctcatc aagtgaatgt ttctgcgcaa ggaatgcccg tccaggtgca
     5281 ggcggatcca cttcatcttc cttttgccga taaatccgtt gatatttgtc tactggcaca
     5341 taccttgccg tggtgcaccg atccgcatcg tttattacgt gaagccgatc gggtactgat
     5401 tgatgatggc tggctggtga ttagtggctt caaccccatc agtttaatgg gattacgcaa
     5461 acttgtgccg gtattgcgca aaacctcgcc ctataatagc cggatgttta ctctgatgcg
     5521 gcaactggac tggctctctt tgttgaattt tgaagtgctg cacgccagcc gtttccacgt
     5581 tctcccgtgg aacaaacacg gaggaaaact attgaatgcg catattcctg cgcttggttg
     5641 cttacaactt attgttgccc ggaaacggac tattccttta acgctaaatc cgatgaaaca
     5701 gagtaaaaac aagccacgaa ttcgccaggc ggttggagcc acccggcaat gtcgtaaacc
     5761 acaggcttaa acttcgactt ggtagcccgt atcttccagt gtgggattca tcgccgcggc
     5821 acgagccagt tcatcacagc gttcgttttc cgggtgtccg gcatggcctt taacccattc
     5881 ccatttgatt tgatgctgcc ccaatgcagc atcaagacgt tgccagagat cgacattttt
     5941 tactggtttt ttgtctgctg ttttccagcc acgttttttc cagttatgga tccactgggt
     6001 gataccctgg cggacatact ggctgtcggt actcaaaatg acttcgcaat gttcttttaa
     6061 cgcctccagc gcgacaatag cggccatcaa ctccatacgg ttgttggtgg tgcgggtgta
     6121 gcctgcgcta aaggttttct cgcgtccgcg atagcgtaaa atagcgccgt aacccccagg
     6181 tccaggatta cccagacacg aaccatcggt gaaaatttct acctgtttaa gcatctctgg
     6241 tagacttcct gtaattgaat cgaactgtaa aacgacaagt ctgacataaa tgaccgctat
     6301 gagcactgca attacacgcc agatcgttct cgataccgaa accaccggta tgaaccagat
     6361 tggtgcacac tatgaaggcc acaagatcat tgagattggt gctgttgaag tagtgaaccg
     6421 tcgcctgacg ggcaataact tccatgttta tctcaaaccc gatcggctgg tggatccgga
     6481 agcctttggc gtacatggta ttgctgatga atttttgctc gataagccca cgtttgccga
     6541 agtagccgat gagtttatgg actatatccg cggcgcggag ttggtgatcc ataacgcagc
     6601 gttcgatata ggctttatgg actacgagtt ttcgttgctt aagcgcgata ttccgaagac
     6661 caataccttc tgtaaggtca ccgatagcct tgcggtggcg aggaaaatgt ttcccggtaa
     6721 acgcaacagc ctcgatgcgc tatgtgcccg ctacgaaata gataacagta aacgaacgct
     6781 gcacggggca ttactcgatg cccagatcct tgcggaagtt tatctggcga tgaccggtgg
     6841 tcaaacgtcg atggcttttg cgatggaagg agagacacag cagcaacaag gtgaagcaac
     6901 aattcagcgc attgtacgtc aggcaagtaa gttacgcgtt gtttttgcga cagatgaaga
     6961 gctggcagca catgaagccc gtctcgatct ggtgcagaag aaaggcggaa gctgcctctg
     7021 gcgggcataa atacatgtaa aaggcgctaa aaatagcgac ttgggcgatt tttgcagcaa
     7081 acgattcaaa agatgagaaa aatcgttgac gaaggtcgag gcaatccgta atattcgcct
     7141 cgttcccaac ggaacacaac gcggagcggt agttcagtcg gttagaatac ctgcctgtca
     7201 cgcagggggt cgcgggttcg agtcccgtcc gttccgccaa tattcagaaa gcccgaatca
     7261 gtaatgattc gggctttcgt cgttttggtg aagtgagaat ttttcactcc gaagtaaaac
     7321 ggatatgatt taaaaaatgg ccagacatta taaattctgg ctacttctgt tactccagcg
     7381 catcccattt ctccagcagt tctactggaa tttcatttat tacctccgag acgcgttttc
     7441 cgaccaggcg ttctgcctgg cgcagcaaag gaatggtcgg gctgctcggt tcgctgtgct
     7501 caaaccagcg acggatgaca cgcaggcgtt ccagcgcgtc gtttcggtcg cggatctgcc
     7561 ggggttctgc gccggtcatc ggtgatattt cctccgcgat atgcaccggt agcgcaatct
     7621 gctctgcctg catctgttca gtatgaacga tggtggcgtt ttccggctgc tcagcctgtg
     7681 gttgtggcag gatctccggc gtggtgctct gcactgctcc cggtaacagg gccagcagtt
     7741 gcgtcagacg ggagaagtcc ggtgcctggt cattcagcgt ttcacgcgca tggcgttgca
     7801 gccgctcagt ggtctctgct gcctggcgaa aggcatccag cggcagtgtg ccgcgcgctt
     7861 ccaggtctgc cagttgctgg cgtaccgatt ctggtgccag cgcatccgcc gggcgtaatg
     7921 cagatagcga acgctccaca tcccgcacct gaaggcgcat ggcagcatta tttgacagtg
     7981 taatgccacg aatatccgcc attacacctt cgtggtccag caacgccgcc agcgcgttac
     8041 tgcgtgccac cgcagcatct tcggcggtga tatcttcagt cgcgagcagt tgaggatgga
     8101 tggcgtccgg gtacgtggag cacaacgttt ccagcagcgt gagcgcctcc gacagtcccc
     8161 tggcaccggc ctgctggata cggcagcgca gcaggacaac cagaatgcgg atatctttgc
     8221 tgcgcatcag cagacgatgg gcgtcccgct caagctccgc ccagttaatg ttttccggcg
     8281 tactgacaaa atcgccatat tgcgcttctt cacgcggtgc ggcgcgggtg aacagcagca
     8341 ggtattccgg atcgtactcc agatccgggc cgcaggggca ttccgccgaa attgggtcag
     8401 acaggaatat ttccatttaa ttatcctcag tgcgccaggt agtgttctgg ttcgaaaatc
     8461 atgcccgtca ccgggttgtc gtgcatggct ttacccgccc atgcagtcca acccagttgg
     8521 tacggaccac cggtaacagc cggaggggcg gcgtggggct ttagctgtag ctcaatttcc
     8581 cagcagtatt cataaccact aaaggcgcgg accagttctg tcaggacggg caggttattg
     8641 ctgccaggca gaaaacgtcg gtaatcctcc agcgtcagcg ggccgataac cagccggaac
     8701 ttatactgca tgtccggcac ggcctggccg atgagtgcac tattgccaat aacggaagag
     8761 ggggcaggca tccccagccg ggtgatctca tcgtccgcca cggctatcca gtgcaggaca
     8821 tactcctgta tccggaaagg tacaccgaaa tagtgcgcca gcgtggtggc cagaccgtcc
     8881 ggattgcggg actcacgcac cagatgggcc gatgccgcca gacgagcgtg atctggcaac
     8941 gggctgtcag cgctctcccg caaatcctgc ccgctgaggc tggcgacata aaaggcaaag
     9001 cggtcatgtt gcggtttatc cagaccgccc ccagcggact gggcgctgcg ccatgcctgg
     9061 taaaactgcg tcagccagcg gtgatgaaaa agatcagaaa aacgggtcag cgtcggatcg
     9121 cgtcggcttt cagtccggtt acgcgccagt tcggtgtagt gcagcggcag tggaccgttc
     9181 ggcccccaca gcccgaggct gtacagactg atgtgcaggc gcccttcctg ccagcccaca
     9241 ctggcgattt ctcgtggcgc aaaggtcagc gcgggcgttt gtccgaggcg aaatttttcc
     9301 atccgtggtt gctaggtgtt gcccgccgga acggtacaca actgcacatc cacgcggcgc
     9361 atcaggttca gaaaaccgta atgccagggg ggttcctgcg cctgagtgaa atcgttgctc
     9421 atacgcttcc ccgctgtccc gccctgacag gccaggtcat aattttgccg cgctgcatgc
     9481 tgtgcagcgt catctgcgag aaggtattaa tggaaacatg gcgggcgata tagtgttcca
     9541 gcaccaggcc gaacagatac ggactgatgc cggaaaaacc ttcttcatcc accgtcagtt
     9601 cacaactgac gccacgccca tagaccagta gtccggaacc gggcagacgg cgggtgaccg
     9661 gggtcgtttt acaaccaatc aggctgcgca cctggcgcga ttgcggactg tcatgagcag
     9721 gaataaacag gttcagcaga tctcgcaacg cctggccgcc ggtacggtga tccagatcgg
     9781 ccagcggcag ataattaaaa gataactggc ggatcaaccg ccatgccatc tcacgttcag
     9841 ccagcggggg ctgcggcgag cgtggcgggc ggataagccc tacgcccgcc accgggatgg
     9901 ctgcatccac ggttagatcg tcccggccat tgcgggcaat aagacagggc agatcgcggt
     9961 tagtgaccat ggccgtgatg gtgatgtggc gcagattttc cgggtacggc gcttcatact
    10021 ggtcaaccag cgacaggaac acttccgagc ccgtataggg ggtacgggtg ccgtaacggc
    10081 gggcgttttc tgataagcgg cggggctccc ggcgcaggga aaaataacgt ccgtgattgc
    10141 cttcatcatt attgcgggtg tggtagagcg ggcgaaaggc catcttccgt gtggtgtcgg
    10201 tttccagccc ttccacttcc tggaccgaga acacctcata atccagcgga tgggttctgt
    10261 ccaccaccag atgctgttcc gtcgtgctgt gcgtgacatc gatccgggcg gtgacgcggg
    10321 gaaacaggtt gataaccggg gtacagaaca ggctgaactg tgccgcattt gtctggtgga
    10381 tcagccagtc cggcggcagg cggttcagca ggataacaat ctccgccacg ccaccgtcaa
    10441 ttttctgtaa tccggcagac aggcctgtcg gggtgaaaaa atagaagcgt tccggacagg
    10501 caaaatattc atgcagcagg ttatgaccgt gaaacacgtt ccacgccagc ggcagcagcc
    10561 cctgatccgg ctccaggcct tcgtacataa cgggctgttg cagattcaca tccagcgcgc
    10621 cgtcaaaatg ccccggaatg ccagccaggg tcgcgatggc gctggtatgt agaagttcta
    10681 acaggtgtga ggccgtacgc tcctcgccgc agagataaaa cggcagcctg tccagtccgg
    10741 caagctgact gaacgttagt tcaccaaagg tgcgcagggt aatacgcagc gccccggcga
    10801 cgtgaatgtt ggccggcagg tagcggtgca gggcaggcat atccggtggc gcggcggtca
    10861 gtcgcgcctc ttcgatggcg agcggccaca gcgttacatc ctggctgctg cgaaactggc
    10921 aggcagtgct ttcgccttcc ggaatggcgg agaaaaaggc tgtgtcgcgc gggacggtaa
    10981 atcctttcgc cagatcgcct tcttccgtat cgggatgcaa ctgcgccacg ctcattgagg
    11041 gcgtgggtgt gacgtaattg gggctgacca cttccagcgg gcgctgggtg aaacggggga
    11101 attcagcgtc aatttttagc tgcgtgcggg cggtcaggaa gctgaaggcc tcgaccattc
    11161 gctctacata cggatcggca atatccgttc cctgcatgcc cagccgggcg gcgactttgg
    11221 gatgacgggc ggcaaattcg gctcccgttt cccgcagata gctcagttcg cggttgtagt
    11281 atcccagtag ccgtggatcc atcggtctct ctctgaatta aaaaaacgta atgcgactct
    11341 gctccatatc cagcgcgcta cgtacccgga atgcagtcgg atacggctgc gtgaggatct
    11401 cgccgcgaat ttcaaactgt aatgtgttat ggccactctg gcggcgctca tcctgaagtg
    11461 ggatgatttg cagggtggac gctttcagcc ggggttcaaa ccgggtgatg gttcgcaaaa
    11521 tggccctgcg gatatcgtcc cacttgtgct catacagaaa gctgcccgcc agtggcggca
    11581 ggccgtagtt aagtacggac gctgccgcgt acggataacg tgcagcatca atatcatctt
    11641 catggctgac ggtgttgagc agaaatgaca aatcccggcg gatgatctcc ttaatctgaa
    11701 ccgggctgac gctgataccg tggtcgcgtt tctggtttgg ggcgtcgtca cacaggcggt
    11761 caaacagcgt gggcagcagg tgattgcctg gacgctggcg ggaagcgctc acgcgtcgct
    11821 tccttcagcg gtgttaaagg tacaggtgga caaatccagc aggctgatat cgccgtgacc
    11881 ggtgagccac accctctgac ccagcgcctg taccagtgtc tctcccggac cgtcctgcca
    11941 gaccgtttcc cggcacagac gcagggagtc ggcagccttt tcagagccgc tgtagcgggg
    12001 aaacagccag gcactgtgtg tatcgccatt gttcagggtg acgttgacgg gtttccagat
    12061 caaatcgatc agatgcgcag gccgcggcga tttcagcgaa cagatttgtg aaaacggcag
    12121 ccagatatat gctccgcccg cgatcagttc gagtaccgga ccggtacggg agtcgctgtc
    12181 gctaatccag tcaaatgcgc cgccattcca ctgcccggaa gtgtctgtta tggcctcaag
    12241 cgcgcggctg cggtgagcat cagcttcctg cgcttcacca cgggcgttac aggcgagggt
    12301 ggtcagcaga tcctccatcc aggcgggcgg cgggagaata acaccgggcc gctgttcgcc
    12361 ctgaaagcag gcgtggcggt atatctcaca gcggatcagc tcgccaaata cctgcgcttc
    12421 gcgagtataa tttgcgtcca tccgggcgca tagctggatc tgttgcagtg cccgcgacca
    12481 gtcgcctgcc acgcacaata actgaaacag gctgtggcgg cgggaggcct gcgctgggtg
    12541 catcctgacc tgttgctctg ccatctgaat gccctccacc acggaataat cccgcagcag
    12601 cgccgacaag gtaatcggta gctcgttatt gtttgtcatg atatatctct ccatgttgtg
    12661 ccgggtttat ccggtaatgg ttgtcaatgc caatgatgcg atgctctttc cgtgtcagat
    12721 ctggcagtac ggtgttatgc gctgtttccg gggcgtattc cggggaaagt aaatgcagga
    12781 tatcaggggg agtcttttcg acaggccaga tagtttcatc ttcgccagtg gcatccagta
    12841 cattgagaat agcgtcaatg cttaacccac cagtgaccat atcctgcaat gtctcggctt
    12901 cggtgaccag gtcacgcaat gaatccagat cctgcgagga gggctcttcg ttaaaaggaa
    12961 aaggatcagc atggcgatta agatcttgtt cgggagataa caacgcctcg cggtattcct
    13021 gataaagtcg ggtcagggca tttccttttt ccgcaccttc tccggtggag gaggctccat
    13081 gaacaggaat gatatcgaat ggattttgtg atgtcagatg ctgctgcctg aaccaatcca
    13141 gatccagata ttcggttatt gcttccgacg tgatggcagt ctgcgatatc gtttttactg
    13201 tgctcaggtc cggagatgat ggattgccat gtacaaggca ctatgaagat aacccccatt
    13261 ctatcaggtc gccttcattc agacgcatac ggtgatgagg ttccacaatc tggtcgttta
    13321 ccgtacagca atattcggta gactgattga taatccacca tgcttcttcg tggcgggtca
    13381 gagtgagggc aatctttaca tcatcattca ccttaaaaac aggaatccag ggagaggtaa
    13441 cggtgaagat aatttgtgtt ccttcagtcg cgtaaattgt gttatcggcg atcccgtgcg
    13501 cctgtatttt ttggagtccc cacgtaatat ttggtttcat tgtg
//
LOCUS       DAJVQH010000111        13457 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJVQH010000111 DAJVQH010000000
VERSION     DAJVQH010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.111"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..189
                     /locus_tag="OQM87_003209"
     CDS             <1..189
                     /locus_tag="OQM87_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCS1509242.1"
                     /translation="PFVRLDPSRDRSTGGCGLGLAIVHSIALAMGGTVNCDISELGGA
                     RFSFSWPLWHNIPQFTSA"
     gene            265..1194
                     /gene="tus"
                     /locus_tag="OQM87_003210"
     CDS             265..1194
                     /gene="tus"
                     /locus_tag="OQM87_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCS1509243.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(1191..2594)
                     /gene="fumC"
                     /locus_tag="OQM87_003211"
     CDS             complement(1191..2594)
                     /gene="fumC"
                     /locus_tag="OQM87_003211"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCS1509244.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(2737..4383)
                     /gene="fumB"
                     /locus_tag="OQM87_003212"
     CDS             complement(2737..4383)
                     /gene="fumB"
                     /locus_tag="OQM87_003212"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1509245.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            4582..5757
                     /gene="manA"
                     /locus_tag="OQM87_003213"
     CDS             4582..5757
                     /gene="manA"
                     /locus_tag="OQM87_003213"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCS1509246.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLRDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANKSPVTVKGHGRLARVYNKL"
     gene            5858..7366
                     /locus_tag="OQM87_003214"
     CDS             5858..7366
                     /locus_tag="OQM87_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1509247.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7592..8857)
                     /gene="uidC"
                     /locus_tag="OQM87_003215"
     CDS             complement(7592..8857)
                     /gene="uidC"
                     /locus_tag="OQM87_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCS1509248.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSIRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(8896..9063)
                     /locus_tag="OQM87_003216"
     CDS             complement(8896..9063)
                     /locus_tag="OQM87_003216"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCS1509249.1"
                     /translation="MGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKK
                     VQQQLISDITN"
     gene            complement(9079..10269)
                     /gene="uidB"
                     /locus_tag="OQM87_003217"
     CDS             complement(9079..10269)
                     /gene="uidB"
                     /locus_tag="OQM87_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCS1509250.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQV"
     gene            complement(10266..12077)
                     /gene="uidA"
                     /locus_tag="OQM87_003218"
     CDS             complement(10266..12077)
                     /gene="uidA"
                     /locus_tag="OQM87_003218"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCS1509251.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(12465..13055)
                     /gene="uidR"
                     /locus_tag="OQM87_003219"
     CDS             complement(12465..13055)
                     /gene="uidR"
                     /locus_tag="OQM87_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCS1509252.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(13284..>13457)
                     /locus_tag="OQM87_003220"
     CDS             complement(13284..>13457)
                     /locus_tag="OQM87_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1509253.1"
                     /translation="WSHYPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQ
                     ILTVSGGGVQELN"
BASE COUNT         3387 a         3523 c         3193 g         3354 t
ORIGIN      
        1 ccttttgttc gcctcgatcc cagccgggat cgctcaaccg gcggctgcgg gctggggctg
       61 gcaattgtcc actctatagc actggcaatg ggcggtacgg ttaattgtga catcagcgaa
      121 ctgggtggtg ctcgcttctc gtttagctgg ccgttatggc ataacatccc gcaatttacc
      181 tctgcctgac actacgcgca cgatggtcaa gtcacaacga ctgtgctata aaataagtat
      241 gttgtaacta aagtggttaa tattatggcg cgttacgatc tcgtagaccg actcaacact
      301 acctttcgcc agatggaaca agagctggct acatttgccg ctcatcttga gcaacacaag
      361 ctattggttg cccgcgtgtt ctctttgccg gaggtaaaaa aagaggatga gcataatccg
      421 cttaatcgta ttgaggtaaa acaacatctc ggcaacgacg cgcagtcgct ggcgttgcgt
      481 catttccgcc atttatttat tcaacaacag tccgaaaatc gcagcagcaa ggccgctgtc
      541 cgtctgcctg gcgtgttgtg ttaccaggtc gataaccttt cgcaagcagc gttggtcagt
      601 catattcagc acatcaataa actcaagacc acgttcgagc atatcgtcac ggttgaatca
      661 gaactcccca ccgcggcacg ttttgaatgg gtgcatcgtc atttgccggg gctgatcacc
      721 cttaatgctt accgcacgct caccgttctg cacgaccccg ccactttacg ctttggttgg
      781 gctaataaac atatcattaa gaatttgcat cgtgatgaag tcctggcaca gctggaaaaa
      841 agcctgaaat caccacgtag tgtcgcaccg tggacgcgcg aggagtggca aagaaaactg
      901 gagcgagagt atcaggatat cgctgccctg ccacagaacg cgaagttaaa aatcaaacgt
      961 ccagtgaagg tgcagccgat tgcccgcgtc tggtacaaag gagatcaaaa acaagtccaa
     1021 cacgcctgcc ctacaccact gattgcactg attaatcggg ataatggcgc gggcgtgccg
     1081 gacgttggtg agttgttaaa ttacgatgcc gacaatgtgc agcaccgtta taaacctcaa
     1141 gcgcagccgc ttcgtttgat cattccacgg ctgcacctgt atgttgcaga ttaacgcccg
     1201 gcttttatac tgccgaccat ctgttctggc cgtacccagc tgtcaaactc ggcttcgcta
     1261 agatacccca gcgcaagggc cgcagctttt aaggtcagcc cttctttatg cgcttttttg
     1321 gcgatctccg cggctttgtc ataaccaatg tgggtgttaa gcgcagtcac cagcatcagc
     1381 gattcattga gtaattgatt gattcgctca cgattcggtt caatacccac tgcgcagtgt
     1441 ttgttaaaac tttccatgcc atctgccagc aagcgcaccg attgcaggaa attatggatc
     1501 accatcggac ggaagacgtt cagttcaaag ttaccggaag cgccacccat gttgatcgcc
     1561 acgtcgttcc ccattacctg acagcagagc atggttaatg cttcacactg ggttgggttc
     1621 actttccccg gcatgattga gctgcccggc tcattttccg ggattgagat ttcaccaatt
     1681 ccgcagcgcg ggccagaggc cagccagcgg acatcattgg cgattttcat cagtgacgca
     1741 gccaaccctt tcaacgcgcc gtgcgcctga accagggcat cacaggtcgc aagcgcttca
     1801 aatttgttcg gcgcggtaac aaacggtgca caggtaatga ctgccagttc atctgctacg
     1861 cgccgcgcat actccggatg ggtatttagt ccagtaccca ccgctgtacc gccaagagcc
     1921 agttccgcta cgtgaggcag gctgtattcg atatgtttga gattatgctc gagcatcgct
     1981 acccagccgg aaatctcctg ccctagtgtt agcggcgtgg cgtcctgcaa gtgggttcga
     2041 ccgattttga cgatatcggc aaatgcacgc gatttttcac tcagcgtctg tgtcagggtt
     2101 ttaagctgcg gaatgagttg cttgcgcagt gccagcagcg ccgcaacgtg catcgccgtc
     2161 ggaaagacat cgttggaact ttggcttttg ttcacgtcgt cgttagggtg aactttacgt
     2221 tccatcccgc gcacaccgcc gagtaattca ctggcccggt tagccagcac ttcgttcatg
     2281 ttcatgttac tttgcgtgcc ggagccggtc tgccagatag ccagcgggaa ttcgtcgtca
     2341 tgctgtcctg ccagtacttc atccgccgcc tgccgaatgg cgctcgcttt ctcttcagac
     2401 aacaagtcta aatcttcatt aacttttgcc gctgcgcgct tggttagcgc cagcgcatga
     2461 atcagtgagg tgggcatttt ctccgtcgaa atgcggaaat gctccagcga gcgttgagtt
     2521 tgtgcgcccc acagcttatc tgccgggaca tcaatcgccc ccatcgaatc tttttcgctg
     2581 cgtactgtat tcatggcctg ctcctcacct gattaatttt ttctttctgt tttgctttcg
     2641 ttaagcaact tttagctcac tcattattta ctatttgata acaaatgttt ggtctttcgt
     2701 gccatgtaaa aaaaccgccc cgaagggcgg ctctgtttat ttcacacagc gagtgcactg
     2761 tgtgagttgt atctgctgga agaagtcatt tcctttatca tccacaagga taaacgccgg
     2821 gaaatcttcc acttcaattt tccagatggc ttccattccc agttccggat attcaacaca
     2881 ttccaggctc ttaatacttc cctgcgccaa tacagcggcc ggaccaccga tactgccaag
     2941 gtagaagccg ccgtgttttt tacaggcatc cgtcacctgc tggctgcggt tgcctttcgc
     3001 cagcatgatc atacttccgc cctgcgcttg cagttgatcg acataagaat ccatccgtcc
     3061 ggcggtcgtt gggccaagag aaccggaggc ataaccttcc ggcgttttgg ccggacccgc
     3121 gtagtaaatc ggatgatctt taatgtattg cggcagccct tcaccgttat ccatccgctc
     3181 tttcagtttg gcgtgagcaa tatcacgacc aacgataatc gtgccgttaa gcgataagcg
     3241 tgtagaaacg ggatactgcg acaactgtgc gaggatctct ttcatcggac ggttaaggtc
     3301 aacgcgcacc gcttcgcctt ctcccgcttt acgcagctct tcagggatat atttacctgg
     3361 attatgttcc agtttttcga tccagatccc ctgacggttg atcttcgctt tgatattacg
     3421 gtcagcagag caggaaacac ccataccaac cggacaggat gcgccgtgac gtggcaggcg
     3481 aatcacgcgg atgtcgtgag cgaagtattt accaccaaac tgcgcaccca gaccgagatt
     3541 ttgcgcttcg atcagtaatt ctttttccag ttccacatcg cggaacgcct gaccgtgctc
     3601 attcccttcc gttggcagtt catcatagta tttcgcggaa gccagtttca ccgttttaag
     3661 gttcgtttct gcagaagttc caccaataac gaacgcaata tgatacggag gacaggccgc
     3721 ggtacccagc gtgcgcatct tctcaaccag gtaatttttc agtttccccg gcgtcagtaa
     3781 cgctttggtt tcctgataga gatacgtctt gtttgccgaa ccaccacctt ttgcgataca
     3841 gaggaatttg tattcgtcgc catcaacggc ataaagatcg atctgcgctg gcagattggt
     3901 gccggtattc acttctttat acatatccag cggcgcgttt tgcgagtagc gcagattatc
     3961 ttcgatataa gtgttataga caccgcgcgc cagcgccgct tcatcaccac caccggtcca
     4021 tacacgctgc ccttttttac caacaataat cgcggtgccg gtatcctgac aggttggcag
     4081 aacgcctttc gccgcgatgt cggagttacg caggaattgc agcgccacat atttatcatt
     4141 ttcgctggcc tccgggtcac gcagaatgtc ggccacctgt tgttggtgag ccggacgcag
     4201 cataaacgac gcatcatgaa acgcctgacg cgccaacaga gttaacgctt cgggtgcgac
     4261 tttcaaaatc tcctgccctt caaattcaga tacgctaacg tgttcactgg ttagcaggta
     4321 atactcagta tcatcttttt tgagtggaaa aggagactga taatgaaagg gtttgtttga
     4381 cattgttctc tcacttactg cctggtttgg ttatgctctg ggcgggtgtt ccgttgccct
     4441 gttaaaagcg agtaacaata tcctacacag ttttttaaca aaaactgaga ctagtacgac
     4501 tttttgcggc tccaggttac ttcccgtagg attcttgctt taatagcggg attaatttcc
     4561 acattaaaac agggattgat catgcaaaaa ctcattaact cagtgcaaaa ctatgcctgg
     4621 ggtagcaaaa cggcgttgac tgaactttat ggtatggaaa atccgtccag ccagccgatg
     4681 gccgagctgt ggatgggcgc acatccgaaa agcagttcac gagtgcagaa tgccgccgga
     4741 gatatcgttt cactgcgtga tgtgattgag agtgataaat cgactctgct cggagaggcc
     4801 gttgccaaac gctttggcga actgcctttc ctgttcaaag tattatgcgc agcacagcca
     4861 ctctccattc aggttcatcc aaacaaacac aattctgaaa tcggttttgc caaagaaaat
     4921 gccgcaggta tcccgatgga tgccgccgag cgtaactata aagatcctaa ccacaagccg
     4981 gagctggttt ttgcgctgac gcctttcctt gcgatgaacg cgtttcgtga attttccgag
     5041 attgtctccc tactccagcc ggtcgccggt gcacatccgg cgattgctca ctttttacaa
     5101 cagccacatg ccgaacgttt aagcgaactg ttcgccaacc tgttgaatat gcagggtgaa
     5161 gaaaaatccc gcgcgctggc gattttaaaa tcggcgctcg acagtcagca gggtgaaccg
     5221 tggcaaacga ttcgtttaat ttctgaattt tacccggaag acagcggcct gttctccccg
     5281 ctgttactga atgtggtgaa attgaatcct ggcgaagcga tgttcctgtt cgctgaaaca
     5341 ccgcacgctt acctgcaagg cgtggcgctg gaagtgatgg cgaactccga taacgtgctg
     5401 cgtgcaggtc tgacgcctaa atacattgat attccggaac tggttgccaa tgtgaaattc
     5461 gaagccaaac cggctaacca gttgttgacc cagccggtga aacaaggtgc agaactgggc
     5521 ttcccgattc ctgtggatga ttttgccttc tcgctgcatg accttaggga taaagaaacc
     5581 accatcagcc agcagagtgc cgccattttg ttctgcgtcg aaggcgatgc aacgttgtgg
     5641 aaaggttctc agcagttaca gcttaaaccg ggtgaatcag cgtttattgc cgccaacaaa
     5701 tcaccggtga ctgtcaaagg ccacggccgt ttagcgcgtg tttacaacaa gctgtaagag
     5761 cttactgaaa aaattaacat ctcttgctaa gcttattaaa ggcttataac accttcaggc
     5821 ggccagtccg cctgatttca ttttatggat aatcattatg aataaatcgc tggtagcggt
     5881 aggcgtcatt gttgcgctag gcgtagtctg gacaggcggc gcatggtata caggcaagaa
     5941 gattgaaacc catctcgaag acatggtcgc gcaggcgaac gcgcaactca aactgacagc
     6001 tcctgaatcc aacctggaag tgagttatca aaactatcat cgcggcgtat tcagcagcca
     6061 gttgcaactg ttggtgaaac ccattgctgg gaaagaaaat ccgtggatta aaagcggtca
     6121 gagcgtcatc ttcaacgaat cggttgatca tggtcccttc ccgcttgccc agcttaaaaa
     6181 actgaacctg atcccgtcga tggcatcaat tcaaaccacg ctggttaata acgaagtaag
     6241 caaaccactg tttgatatgg caaaaggtga aacgcctttt gagattaact cgcgcattgg
     6301 ttacagcggt gattccagtt ccgatatttc gctcaatcca ctgaattacg agcaaaagga
     6361 tgaaaaagtc gcctttagcg gcggcgagtt ccagttaaat gctgacagag acggcaaagc
     6421 catctccctt tccggggagg cgcaaagtgg tcggatagac gcagttaacg aatacaacca
     6481 gaaagtgcag ttgaccttta ataatctgaa aaccgacggt tccagcacgc tgggaagttt
     6541 tggtgagcgc gtaggaaacc aaaaactgtc actggaaaaa atgaccattt cagtggaagg
     6601 caaagaactg gcactgctgg aaggcatgga gatcagcggt aaatcggatc tggtcaatga
     6661 cggtaaaacg atcaatagcc aactggatta ctcgctaaac agcctgaagg tacagaatca
     6721 ggatctgggc agcggcaagc tgactttaaa agtcggccag attgatggtg aagcctggca
     6781 tcagtttagc cagcaatata acgcgcaaac tcaggcgctg ctggcacagc cagaaattgc
     6841 caacaatccc gaactttatc aggagaaagt gacggaagcc ttctttagcg ccctgccgct
     6901 gatgttgaaa ggcgatccgg tgattactat cgcgccgcta agctggaaaa acagtcaggg
     6961 tgaaagtgcg ctgaatctgt cgctgttcct gaaagatccg gcaacgacta aagaagcgcc
     7021 gcaaacgctg gcgcaggaag tagatcgttc ggttaaatct ctggatgcga aactgaccat
     7081 tccggtggat atggcaactg agttgatgac tcaggtagcg aagctggaag gttatcagga
     7141 agatcaagcg aaaaaactgg cgaaacagca agttgaaggt gcatcagcaa tggggcagat
     7201 gttccgtctg accaccttgc aggacaatac catcaccacc agcctgcaat atgctaacgg
     7261 tcagataacg ttaaatgggc agaaaatgtc gttagaagat tttgttggca tgtttgcgat
     7321 gccagctctt aacgttccgg ctgtaccggc aattccgcag cagtaattca cggccaaaac
     7381 gccggagaat tttcactccg gcgttttcat ctgaaaacaa accagagatt gcctgatgcg
     7441 ctacgcttat caggcctacc ttttcctgca acactttgaa tttatgagtt tttgtaggct
     7501 ggataaggcg tttacgccgc atccggcata aaaaacgcgc actttgtcaa caatctgaaa
     7561 cgccggaggt ttttctctcc ggcgttattt cttacttcag cataaagtca tactgaatac
     7621 tcattttcac atcccagtaa ttcaggcgat cgcccagcgt actgccatcg cgcttttccc
     7681 gccctttatc ggcagcaaga aagaaattca tcccttgtaa cggcccttcg ctgacccgat
     7741 agttcatacc acctaagata cccgcgcctt caatccgcac atctcgttta ctggattcat
     7801 agccgcgagt cacaatcggt gcaaatgtca gcgtaaattg cggagttaca cggtagttga
     7861 cacctaattg ccaggactgc attccttcac ggttacgatc aagacttaat gaaaagggat
     7921 agccaatgtc ggtatccacc acggcaccat tcattgcatg gcgcaaataa ccaccactgc
     7981 caaatacgct tccccacggc gcattccagg tcaattgtcc actaaccaac gccgtttcat
     8041 tgggctggct ggtattacga cttaaacctt cgagctgcgc gtaaaagccg agcaactccc
     8101 ctttcaacgt ataattcggg tcgagcggta tgcttttctc gagaatactt tgtatctgat
     8161 tggaaacatc tttctgtacc gatgccccca gagaatagcg actggtatca tcatgccaac
     8221 tggcggctaa aaaagaacgt gcgcgatgtt tatcgtattt tcccgtctgg ctgttataca
     8281 ccccttcatc acgaataccg gttgttgaat cagtccataa aaatactcgt gaacgccaca
     8341 ttccccacag atcaatattt ggtgttaaag cccaataacc taatgctcct tcagaaactg
     8401 tcggtaatgt acgttgcgaa ctgccagcca ttaacggaat acgataaggt aagctaccat
     8461 aactataatc cgtcccgaag cgacctaatt taagcaggct attttcactg ggttttattt
     8521 ttactgcccc cagggcaaag ccaaaacttt tatcaccatc aagataccac cggatactca
     8581 tatcagcacg agcacctaat ttataaacat aatacgctcc gccttcaacg ccaataatat
     8641 tggaataata accactattg aaatcgagca atcctccctg tacccatgcg taaatatctc
     8701 ttcccgcgcc agcactgggc ttatcagccc tgcgcaattc gtttttcagt ctgatgcgta
     8761 gtcccgcttc atcgtcagcc agttgcgcca cgtaagcaga ggtaaggcca gaggcagccg
     8821 tcaggcaaat aacggccatg gccactattt ttctcatagt tgcacctttg atgttctgat
     8881 tatttttatt gaatattaat tagtgatatc gctgattaat tgctgctgca cttttttacg
     8941 attatcaatt tcaaccacga tttctttgaa ttttttatcc gtgagcggat aaaaccagat
     9001 aataacgaat gccagtagca taaatccgca aggtactaag gcaattgatg tgcggatgcc
     9061 cataataact tccggcgttt acacctgatt ggcgatatat ccgcttaatc ccaaaataaa
     9121 ggcaggaatt gaacctccga ttgcctgacc gcatttacgg gtaaatgaga atagtgaata
     9181 ggtgagccct tcaattcgca cgccggtcag gtattcaccg tattctacgg tatcagcttc
     9241 cagtgcccac atcacagtca tagtaacgcc ctgaccaatt gaagcgatgg ccaacgcaac
     9301 caacgccacc ggcagtgacc agacggaaac ccagaagaat agcagataac cgcaggttcc
     9361 cagcaaagcg ccaatcagga aggtattctt tttaccgatc ctcgcgacca tccccggcac
     9421 aagcggtgcc gatgccacag taccaaccag gttttgcacc agtaccagca cagtgaacag
     9481 cccggtatca tttaacacat agcgcacgta gaacaacgac gaggcgctga ccgcgaaggt
     9541 cgaaatcagc acacacagcg caccgatgca caacataaac agcgggcgat tccgtttcag
     9601 ggtttgcaaa ctgatcttca atgacggctg cgccacgata cgtaccacat tttcacgcgt
     9661 cgatttgaag cagatgaagt aaagcaccat tccggcaatc gccagcacaa tcgtccagaa
     9721 atggtatacc gacaccatct cttccgggct ggagttctta atgctcggtc ctatcagaaa
     9781 tgccaggcat acaaaggtca atgaagcggc aatcccacga gccgcgccca gacgggcgcg
     9841 agattgtggt tgttgggtca tcgcggtagc aagtgaacca taaggaatat tcaccaggct
     9901 gtagcaaagc ccgaggccca tgtaggtcaa atatgcatac accactttgc taccatggct
     9961 ccagtcggtc ggtacccaga ataccagcac gctgaagatc attaacggcg cagtaccgaa
    10021 gagtaaaaac gggcggaatt ttccccagcg ggtattcaca ctgtccacca ctcgtccggc
    10081 aaagacgtcg gcgaaggcat caaatacccg caccagtaac agcatggtgc ctgccgcagc
    10141 ggcaccgacg ccagcgacgt cggtgtagta actcaatagg aagagcgccc ccattgcgaa
    10201 ggcgaagtta ttggcgacgt caccgaggct gtagccgacg atggtgcgcc aggagagttg
    10261 ttgattcatt gtttgcctcc ctgctgcggt ttttcaccga agttcatgcc agtccagcgt
    10321 ttttgcagca gaaaagccgc cgacttcggt ttgcggtcgc gagtgaagat ccctttcttg
    10381 ttaccgccaa cgcgcaatat gccttgcgag gtcgcaaaat cggcgaaatt ccatacctgt
    10441 tcaccgacga cggcgctgac gcgatcaaag acgcggtgat acatatccag ccatgcacac
    10501 tgatactctt cactccacat gtcggtgtac attgagtgca ggccggctaa cgtatccacg
    10561 ccgtattcgg tgatgataat cggctgatgc agtttctcct gccaggccag cagttctttt
    10621 tccagtacct tctctgccgt ttccaaatcg ccgctttgga cataccaacc gtaataacgg
    10681 ttcaggcaca gcacatcaaa gagatcgctg atggtatcgg tgtgagcgtc gcagaacatt
    10741 acattgacgc aggtgatcgg acgcgtcggg tcgagtttac gcgttgcttc cgccagtggc
    10801 gcgaaatatt cccgtgcacc ttgcggacgg gtatccggtt cgttggcaat actccacatc
    10861 accacgcttg ggtggttttt gtcacgcgct atcagctctt taatcgcctg taagtgcgcc
    10921 tgctgagttt ccccgttgac agcctcttcg ctgtacagtt ctttcggctt gttgcccgct
    10981 tcgaaaccaa tgcctaaaga gaggttaaag ccgacagctg cagtttcatc aatcaccacg
    11041 atgccatgtt catctgccca gtcgagcatc tcttcagcgt aagggtaatg cgaggtacgg
    11101 taggagttgg ccccaatcca gtccattaat gcgtgatcgt gcaccatcag cacgttatcg
    11161 aatcctttgc ctcgcaaatc cgcatcttca tgacgaccaa agccagtaaa gtagaacggt
    11221 ttgtggttaa tcaggaactg ttcgcccttc actgccactg accggatgcc gacgcgcagc
    11281 aggtagatat cacactctgt ctggcttttg gctgtgacgc acagttcata gagataacct
    11341 tcacccggtt gccagaggtg cggattcacc acttgcaaag tcccgctggt gccttgtcca
    11401 gttgcaacca cctgttgatc cgcatcacgc agttcaacgc tgatatcacc attggccacc
    11461 acctgccagt caacagacgc gtggttacag tcttgcgcga catgcgtcac cacggtgata
    11521 tcgtccaccc aggtgttcgg cgtggtgtag agcattacgc tgcgatggat cccggcatag
    11581 ttaaagaaac catggaagta agactgcttt ttcttgccgt tttcgtcggt aatcaccatt
    11641 cccggcggga tagtctgcca gttcagttcg ttgttcacac aaacggtgat acgtacactt
    11701 ttcccggcaa taacatacgg cgtgacatcg gcttcaaatg gcgtatagcc gccctgatgc
    11761 tccatcactt cctgattatt gacccacact ttgccgtaat gagtgaccgc atcgaaacgc
    11821 agcacgatac gctggcctgc ccaacctttc ggtataaaga cttcgcgctg ataccagacg
    11881 ttgcccacat aattacgaat atctgcatcg gcgaactgat cgttaaaact gcctggcact
    11941 gcaattgccc ggctttcttg taacgcgctt tcccaccaac gctgatcaat tccacagttt
    12001 tcgcgatcca gactgaatgc ccacaggccg tcgagttttt tgatttcacg ggttggggtt
    12061 tctacaggac gtaacataag ggactcctca ttaagataaa aatactggtc aacctttaat
    12121 ctgattagtt aaccaattct gtgttgcaca cagttatctg tgagaaaccc atggaagaga
    12181 aatggaaata aaaagaactg acaccgcgat caaaaaacca aaaactgcaa tacccacagc
    12241 gtgatatgga tcgcattaat ctttaaaaca gcgttgacag aagccataaa acaagcaatc
    12301 gagcgttcgt tcgagagtat tgggaacgat aaactctcat gacatatggc tacggtgaat
    12361 attttggggg ggaattctcc gaacacgggg aggataattt ttgcacagag aaattacgat
    12421 atgaaacgcg ggcacagtta tcaccagccc gcgaaatgag aggatcagga tgcggttaat
    12481 ataccgccaa tcatcgcgcg taatccctga gcaaacgacg cttcacgcgc caggtcttcc
    12541 gcttcgatat cggccagtac accgtaggtc agatccagca gtaaacgtga agtcattgcc
    12601 gtgttgatgt ctgggcttat ctcgcctttt tgctgggcat cacgcatccg ctgggcaaca
    12661 aattcagtga tcgtcatatg cttattttta agcatggcgg cgacctgtgg gttacgcatc
    12721 ccttccgcca taatttcgac caccagcgcc cgttgcccaa aggcttcatg ggtgagagag
    12781 acaatagact cgaccatata gtcaacaaaa tgaatccctt caatcggttc ccggaaacga
    12841 gccagcgccc tctcctggtc ctgtaagata atcgcctgaa tcaaggcttc tttggagatg
    12901 aaatggtgat agagcgtccc aggactaatg gcgcaagatt tacagatggc tttcatcgag
    12961 gcactgtgaa atccattttc tgaaaaaatc tctctggcag cattgaggat ccgggtccgt
    13021 gtcggttgtg cttcagtctg catgttatcc atcattaaac cattatagtt agcgtgaata
    13081 actagtttac cgcgttgtca ggtgagcgcc tatcattgct gttgaatagt gcgaaggtac
    13141 actctattcg tatacttttc acccagcaat tggttaacca attgccgtga tttggctgcc
    13201 agttgtttag cagagacgtt gatgaattgc acacgctatc ccggtaaaca acacatacga
    13261 ccgtttaccg gttcgttagt gtattaattg agctcctgta ccccaccacc ggagacggtg
    13321 agaatttgtc cgcttaccca gctggctgca ggcgagcaaa ggaacagcgc cgcgttagca
    13381 atatcttgcg gttggcccag acgtctgatc ggcgtgtgct gtaacatttt ttgttcaatt
    13441 tctgggtaat gactcca
//
LOCUS       DAJVQH010000112        13444 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJVQH010000112 DAJVQH010000000
VERSION     DAJVQH010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.112"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..353
                     /locus_tag="OQM87_003221"
     CDS             <1..353
                     /locus_tag="OQM87_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509254.1"
                     /translation="FRKECPLNSGYLRALDNVLQELGRERTIAMSLPEFEQSLFMAAQ
                     PDNLLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIV
                     WLRETIKNLYASMA"
     gene            510..1259
                     /locus_tag="OQM87_003222"
     CDS             510..1259
                     /locus_tag="OQM87_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCS1509255.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1281..1847
                     /gene="yieF"
                     /locus_tag="OQM87_003223"
                     /gene_synonym="chrR"
     CDS             1281..1847
                     /gene="yieF"
                     /locus_tag="OQM87_003223"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCS1509256.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1901..3238)
                     /gene="adeP"
                     /locus_tag="OQM87_003224"
     CDS             complement(1901..3238)
                     /gene="adeP"
                     /locus_tag="OQM87_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCS1509257.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3403..4068
                     /gene="yieH"
                     /locus_tag="OQM87_003225"
     CDS             3403..4068
                     /gene="yieH"
                     /locus_tag="OQM87_003225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCS1509258.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            4135..4608
                     /locus_tag="OQM87_003226"
     CDS             4135..4608
                     /locus_tag="OQM87_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCS1509259.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            4657..5244
                     /locus_tag="OQM87_003227"
     CDS             4657..5244
                     /locus_tag="OQM87_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCS1509260.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(5306..6028)
                     /locus_tag="OQM87_003228"
     CDS             complement(5306..6028)
                     /locus_tag="OQM87_003228"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1509261.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPILGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNMLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6030..7205)
                     /locus_tag="OQM87_003229"
     CDS             complement(6030..7205)
                     /locus_tag="OQM87_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCS1509262.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYLLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            complement(7232..8848)
                     /gene="bglH"
                     /locus_tag="OQM87_003230"
     CDS             complement(7232..8848)
                     /gene="bglH"
                     /locus_tag="OQM87_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCS1509263.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(8934..10331)
                     /gene="bglB"
                     /locus_tag="OQM87_003231"
     CDS             complement(8934..10331)
                     /gene="bglB"
                     /locus_tag="OQM87_003231"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCS1509264.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            complement(10347..12224)
                     /gene="bglF"
                     /locus_tag="OQM87_003232"
     CDS             complement(10347..12224)
                     /gene="bglF"
                     /locus_tag="OQM87_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCS1509265.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(12358..13194)
                     /gene="bglG"
                     /locus_tag="OQM87_003233"
     CDS             complement(12358..13194)
                     /gene="bglG"
                     /locus_tag="OQM87_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCS1509266.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         3551 a         3374 c         3080 g         3439 t
ORIGIN      
        1 atttcaggaa ggagtgtccg ttaaactcag gctacctcag ggcgctggac aatgtgttac
       61 aggagctggg gcgcgaacgc acgattgcta tgagcctgcc ggaattcgag cagtcactgt
      121 ttatggcagc gcaacccgac aatctgctac tggcgaccgc gccgcgctac tgtcagtact
      181 acaatcaact ccatcaactg ccgttggttg ctcttcctct cccgtttgac gaaagccagc
      241 aaaaaaagct ggaagttcct tttaccctgc tgtggcataa acggaacagc cataatccga
      301 agatcgtctg gttacgggaa accattaaaa acctttacgc gtcgatggca taaccgaatc
      361 gtatgaaacg cgacccaatt tcacaataaa atgtaaaaaa gttgtaataa gcttgtctga
      421 atcgaacttt tagccgcttt agtctgtcca tcattccagt aaatgattac tcttgtattc
      481 ataatggacc attaagcatg gagcgaaaaa tggcgactca ctttgcccga gggattttaa
      541 cggaaggaca tctgatttct gttcgtctcc cctcccagtg tcatcaagaa gcccgaaaca
      601 ttccccctca tcgtcaaagc cgttttctgg cgtccagagg tttactcgca gaactgatgt
      661 tcatgctgta tggcattggc gaattgccgg aaatcgtcac cctgccgaaa ggtaaaccgg
      721 ttttcagtga taaaaatttg ccttcgtttt ctatttccta tgccgggaat atggttggcg
      781 tggcgttaac aaccgaaggt gaatgtggcc tcgatatgga actacagcgt gcgacgcgcg
      841 ggtttcatag cccacacgcg cccgataacc acaccttttc cagcaatgaa tcgctatgga
      901 tcagtaaaca aaacgatcct aacgaagcgc gggcgcagct cattacgctg cgccgaagcg
      961 tgctaaaact aaccggtgat gttttgaatg acgatccgcg cgatctgcag ctgctgccca
     1021 ttgccggacg cctgaaatgt gctcatgtaa atcatgtaga agcgttatgc gacgcggagg
     1081 acgtgctggt gtggtccgtg gcggtcacgc ccgcgattga aaagctcagt gtctgggagt
     1141 tagatggcaa acacagctgg aaaagcctgc cggatattca cagccgcgcc aacaatccta
     1201 ccagccggat gatgcgtttt gcccagctct ctaccgtgaa gtctttttcg ccaaattgat
     1261 agacaaccac aggagtcatt atgtctgaaa aattgcaggt ggttacgtta ctggggagcc
     1321 tgcgcaaagg ctcatttaat ggcatggttg cacgtaccct gccgaaaatt gctccggcga
     1381 gcatggaagt caatgcgtta ccttccattg ccgacattcc cctgtatgac gctgacgtac
     1441 agcaggaaga aggttttcca gcaacggttg aagctctggc ggaacagatc cgtcaggctg
     1501 acggtgtggt gatcgtcacg ccggaatata actactcggt accgggtggg ctgaaaaatg
     1561 ccatcgactg gctttcccgc ctgccggatc aaccgctggc cggtaaaccg gtgttgattc
     1621 agaccagctc aatgggcgtg attggcggcg cgcgctgtca gtatcacctg cgccagattc
     1681 tggttttcct cgatgcaatg gtgatgaaca agccggaatt tatgggcggc gtgattcaga
     1741 acaaagttga tccgcaaacc ggagaagtga ttgatcaggg tacgctggac cacctgaccg
     1801 ggcaattgac cgcatttggt gagtttattc agcgagttaa gatctaaata aaaaacccgc
     1861 cagtaatcat gcatggcggg tttttaatgc gctatcgatt ttaatgagcg tcgataaata
     1921 caatcttcag gataaacagc agcgcaacga tgattacgca cgggctaagg tcacgcagac
     1981 gcccggtacc aatcttcatc acgcagtagg agataaagcc cagcgcaata ccttcggtaa
     2041 tcgaaaagct gaacggcatc atcacggcgg taataaacgc cggaacagat tcagtaagat
     2101 cctgccagtt cacgcgagca agacttgagg tcatcaacac gccaacgtag atcaacgcgc
     2161 cagctgcagc gtagcctggc accattcccg ccagcggcga cagaaagata accagcagga
     2221 acagcagacc aacaaccact gccgtcagac cggtacgacc gccaaccgat acgccggaag
     2281 aggactcaat ataagccgta acggaagaag taccgataaa cgaaccggtc acggaagaga
     2341 tactgtcgac atacagcgcc tgcttcatgc gcgggaattt ccccttctca tccgccagac
     2401 ctgctttatc ggtcacgcca atcagcgtac cggaggagtc aaacaagttg accaacatga
     2461 aagagaaaat cacccctgcc agcccgaggt taaacgaccc ggctaaatct acatgaccca
     2521 caactgtcat tacgctcggc ggcgcagaaa cgatgccatt gtagtgcaca tcacccagca
     2581 tccagcccag cagcgtcgtc accacgatag aaaccagcac cgctgcgtga atgttgcgcg
     2641 aggccagaat agcaatgatg aaaaagccga ggatacccag aagtacgctg tgagaagtca
     2701 gattaccgat gctcaccagc gtttccgagt tagcgacaat cacacctgcg tttttcagcc
     2761 ccatcatgcc gataaacagg ccgataccgc tggtaatacc cacacgcaga cttaccggaa
     2821 tgttagcaat catccagtaa cgaacgcgga aaatggtcaa aagaagcagt ccgacagcgc
     2881 cccagaagat tgcgcccata ccgatctgcc acggcagccc cattgcctgg acaaccacaa
     2941 aagcgaaaaa ggcgttaagc cccatcgcag gagccagcgc caccggaagg tttgcaaaca
     3001 atcccatcat aatgctaccg aaagcagcaa tcagacaggt tgttacgaag acggcactgg
     3061 tgtccatgcc agcaacgccc agaatttgtg ggttaacaaa tacgatgtag accatcgtca
     3121 ggaaggtggt gaacccggca atcacttcag ttcgtgccgt agtaccatgt tcgcgcagtt
     3181 taaaaacgcg ttccagcatc ctctgaccag aagtctgggt ggtatgttga tgactcatta
     3241 tctatttccg aacaaggagg gaaaatccgt cgctatcgta taccaaaatg cgacaatagg
     3301 cgcgtttgtg agagactttt ttattggatt tacttatacg gcaacgattg cgttgcgcaa
     3361 atcggctcta cgaaaacgtt aaactgatta aaaaggaaag gcatgtcccg gatagaagcg
     3421 gtatttttcg actgcgacgg tacgctggtc gacagtgaag tcatttgctc ccgcgcatat
     3481 gtaacgatgt ttcaggaatt tggtattacg ctcgatcctg aagaggtatt caaacgtttc
     3541 aaaggtgtaa aactgtacga aattatcgat attgtttccc ttgaacatgg tgttacgtta
     3601 gcgaaaacag aagctgaaca cgtttaccgt gcagaagtcg ctcggctgtt cgattcagaa
     3661 ctggaagcca tcgaaggggc tggagcgctc ctgtcagcga tcactgcgcc aatgtgtgtg
     3721 gtatctaacg gcccaaataa caaaatgcag cattctatgg gcaagctgaa tatgttgcac
     3781 tacttcccgg ataaactgtt cagcggctac gatattcagc gctggaagcc agacccggcg
     3841 ttaatgttcc atgcggcaaa agcgatgaat gtaaatgtag aaaactgcat tctggttgat
     3901 gactcagttg ccggtgcgca atctggtatc gacgcaggta tggaagtgtt ctacttctgc
     3961 gccgacccgc acaataagcc gatcgttcac ccgaaagtca ccacctttac ccatctttcg
     4021 cagttaccgg aactgtggaa agcgcgtggt tgggatatta cggcatagtt cttcacactc
     4081 ccttcactta ccccgcttaa attggcgctc aaaggtaagt aaagggagtt tgatatgtct
     4141 gtttcacgtc gggtaataca tcacggactt tattttgcgg ttttaggacc gttaattggt
     4201 gttctgtttc ttgtcctcta catattcttc gcaaaagaac cgctggttct tttggtaata
     4261 atacaggtac ttccattgtt tctcttattg tcgattacta cgggagctat tcctgcattg
     4321 ttaaccggcg tgatggtcgc ctgtctgccg gaaaagatcg ggtcacagaa aaattatcgt
     4381 tgtctggctg gtggcatagg tggcgtcgtt atcaccgaga tctattgtgc agttattgta
     4441 catattaagg gcatggcttc ctcggagttg tttgaaaaca ttctttctgg tgacagtctc
     4501 gttgtccgca tcattcctgc attgctggca ggtgtggtga tgagcagaat cattacccgt
     4561 ctgcccggat tggatatttc atgtcctgaa acagactctt taagttaagc gggatacttt
     4621 atctttgggc tactcaaaag cagacaggat gttactatga ctcaaaatat caggccgtta
     4681 ccccaattca aatatcatcc caagccactg gaaacaggcg catttgaaca ggataaaacc
     4741 gtagagtgcg attgctgtga acaacagacg tcagtttatt actcgggtcc cttttattgc
     4801 gttgatgaag ttgaacatct ctgtccgttg tgtattgcgg acggttctgc tgctgaaaag
     4861 tttgcaggta gttttcagga tgatgccagc atagaaggtg ttgaatttga gtatgatgaa
     4921 gaggacgaat ttgccggtat taagaacaca tatcctgatg aaatgctgaa agagttggtt
     4981 gaacgcacgc caggttatca tggatggcaa caggaattct ggcttgcgca ttgtggcgat
     5041 ttctgtgctt ttatcggcta tgtgggctgg aatgatataa aagatcgcct cgatgaattt
     5101 gccaaccttg aagaagattg tgagaatttc ggtattagaa attctgatct agctaaatgc
     5161 ctgcaaaagg gtggtgattg tcagggttat ctcttccgct gtctccactg cggcaagctg
     5221 agactgtggg gtgatttttc gtagttattt aaataatgag aacaggccgg agcgtaattc
     5281 atacatccgg ccttatttct tcagcttaac cgaacgctaa ttctgcggca gcggctttat
     5341 cagcgattac catcagcgaa ggatgcagct gcaacacgga agcaggaaca tcttcggtaa
     5401 ccggaccttg cagcatattt ttcagtgcct gcgctttacc tgcgccactg acaattatca
     5461 gcaagttttt cgccgccatg atgcttttcg gccccatcgt cacgtagctg tcaggcacta
     5521 acgaaaagtc gccgcccaac tcaccatgcg ccacaatatc aaccatctct cccaggatcg
     5581 ggaactccac ggtttgctca tggaaatggg tcgtattcgg caaattgcca caaaaatgac
     5641 catctgcacc taatcccagc accaccaaat ccaggccgcc ttcacgcgcc agtttctgat
     5701 catgctcgcg gtagttatca atagtgagct tctggatatt ctcttctttg atccccgcag
     5761 gggtgaaaaa cagattacgc agattggtaa tcgttacgcc ttctccctct ttgccgcgaa
     5821 agggaatttc atcgaaatta tagaaatagc agttatcgta ccaaggctta cctttaacca
     5881 gggtggtgag gtattcatac atgcctttgg gcgtgctacc ggcggtaatt gccaggttaa
     5941 cacgacgcgt cttcgacata taaccaagca gatggtgtgc cgcgacacgg ctcatttcct
     6001 ggtaatcttc ggtaatgatt aatttcatct taatgcctta ttttaaattt ctgtacgaag
     6061 gctgcatagg caggccgcca gacatccatt tcatggttca ggccgggata ttcctggtaa
     6121 tcaaacttaa tttttttctg ctcaagctca gttttcagcc cggcgatatc cttgccggtt
     6181 acgacatctt tatcaccaac gaccacagta aaattacgta gttgctggtt aatagctgcc
     6241 ggatcgttca gccgggccgc gacaccttca tccggtacgg ttgtcgtggt aacaccactg
     6301 aatgtggcca gccagccaaa gctttccaga tgattcattc cggaaaccag cgcctggtac
     6361 ccgccttgtg aaagccctgc cagcgcgcga ccatcggcat ctttacggac actaaaacgc
     6421 ttgctaatca gcgggataat atcgttcatc agttcgcgat ctgccgcttt agcattcagc
     6481 agataaaaga ctttacgtct ttcctgaggc acgaaatctt cgggaataat gcccttcgca
     6541 tcggtttcgg tatccgggat caccaccagc atcggtttaa ttttcccttc agccagcagg
     6601 ttatccatga tttgcgggat acgcccctga tcgatagcgg aacgtccagt atcaccaaaa
     6661 ccgtgataga aatagagcac tggcaaaggc tcgcccatgc cggtgtatcc tggcggggtc
     6721 cagacataca tctgacgttc agattgcaat gcgttggagt ggtaagttat ggcgatcaaa
     6781 tcaccatgcg caacagaacg cgtatccaga taactgccta gcaccagaat catactggag
     6841 ttaacctggc gctgaggctt agtcattgcg gtgcctgtat caatgctgcg tacaccatca
     6901 acattgaaaa aatactcgta caggttgcct ttcaggatag gtgtgcgcca cgaccagact
     6961 cctgcttcgt ctttggtcat cgggtgaata ttgtccggaa ccggaacacc caccactaca
     7021 gagacatttt ttgccccagg ggcaaagtag cgaaaggtca cgctgttatc ggcattgacc
     7081 tgagtcacat actgtttcac cgggatagtg ggtgccgggc tggctggcat atccgctgct
     7141 atggcccact gagcagaaat accgctggca atagccagcg ttaaagcagc aatttttata
     7201 ttcatcgtaa tattcctttc attaagttcc attaccacca gatttcagcc tgggcaccga
     7261 cagcaaactg gtcgtcttta ttgtcttcga aggtgaagcc atccagttcg ttttccaggg
     7321 ctttgatata cgtggcgtag aaacgaattt ccagacgcga ggtcaacata ctggtattga
     7381 ctttaaaggt atggaagagc gtggttttat aaccagactc attaaattta ttactattcg
     7441 catctttgtt ttgctgggtg aaatagccca gttcaacacc tgtctggtta tattggtccc
     7501 aaatataggc cgggcgaaca accgcacgaa tagattcgaa atcagagtgt gcgcctgttt
     7561 cgtagctata aatatcgtta ccgaaggagt aaacaatcgc gttagccaca ataaaatgat
     7621 cgccgatata ggcttcgccc tgcgaggtca gacgaactgc tgttccgccg gtgtgatcac
     7681 cataataacg accattaaag gtggtaaatg gacttgcgcc agcataacgg ccaaagttcc
     7741 tggcgataga gttattcgca accaggaagg agaattcgtt gaagccacct ttatcaaatt
     7801 tctgcgttaa agatgtgccg aacatccagg tatctttcca gtcataatac ccgttattat
     7861 cctgattatc tttttccgat gcgctttcgt ttgccgtgac ataacgacca ctcaccatta
     7921 aggtcgcttt atcccataac gggatatctt tatagcgtaa atcaatagta tgggtattaa
     7981 tctgctgttt gttttgcagg ctgcgatcgt aatcatcgac atcttcgcga accagcgcga
     8041 tatcaatttt acccggaccg actttccagt tttccagacc tacacccgct gcggcatcag
     8101 tacgctgcgt tttccagtcc agcatctgga tttcaatttt cggcgcaccg tgtttaccca
     8161 cccagaaatc agcctctggc gcaaagggca ggaaaccttt ggtggtaacg tacatatcgg
     8221 agaactgcat aaagttctcg ccaccggcgt tatcgccaaa ccagccggta gagtactgct
     8281 gaccaacgtt accatccatc atcacaacgg catcaacccg tttgccgttt tcgttgtaga
     8341 cacgttgttt taactgcaaa tcaaaccagc cggagtattc gttaccaaag cggcccagag
     8401 aaccaatcgc ccatgattta ggtgaaccat gagaggcggt cccccaacca gaacggtaat
     8461 agccgttgta actaaagcca atttcatctt tcacaaactt actgaaatct ttcagagtca
     8521 tggaagaata aatcgagcct gtctcactgg catttttctc ttcatttttg accagtacag
     8581 catcaggttt tgcggcacta ctggtcggga acggattggc ggcataccct tgatcattcg
     8641 tacttacgct acgattcacc gtcgctggcg tatatttttt cttctcttca tctttatact
     8701 ttttgagttc gctttgcgtt tctcttaacg ctttttctaa taactcaagg cgttgttcca
     8761 ccgttaatga ttcagccaat gattgtgcag aaaaggctaa cggtgccatt aataagatgg
     8821 cagaggtaat aagatttcgt ctaaacataa tatcccttta tggtgcaaag aaagaattaa
     8881 cgtatcgcat ccagactgtt ctgaatgcga cgataattaa ggtgctttat tggttatttt
     8941 tttaatgaca gcccccgcgt tttgatcact tctgcatacc atccgaagct tttcttacgc
     9001 gttcttgtga ggcttccttc gccattatca tcacgatcca cataaataaa gccgtagcgc
     9061 ttagacattt gtgaatgaga tgcactgact aaatcaattg gcccccaact ggtgtacccc
     9121 ataatatcca caccatcggc aatcgcttca tttacctgta ccaggtgatc gtttaaatag
     9181 gcaattcgat aatcgtcctg tatcgaacca tccgcttcaa cgctgtcttt tacgcctaat
     9241 ccattctcga caataaataa cggtttttga taacgatccc aaagcgtatt taacagaacc
     9301 cgtaatccaa ccggatcaat ttgccacccc ctctctgaac ttttcagatg cggattgggg
     9361 atcatattca gtatgttgcc ctgcgcattt ttattaatgc tttcgtcgtg ggaaacacaa
     9421 ccagtcatgt aataactaaa agagatgaaa tctacagtat gttttaaatc ttctgcgtca
     9481 ctttcagtca tctcaatggt gatattgtgg tcgcggaaga aacgctgcat atagccggga
     9541 tactggccac gcgcctgaac atcaccaaag aacatccagc gccggttctc ttccatggcc
     9601 tgcaacatat cctgtggctg gcaggtgagg gggtaaacca gcccaccgag aagcatattg
     9661 ccgattttcg cttcggggat caggctatga taggctttaa ctgcccgcgc actggcaacc
     9721 agttgatgat ggatcgcctg ataaacttcc gcctcgccac tctcttctgc cagccccacg
     9781 cccgtgaatg gcgcgtgtaa cgacatattg atttcattaa acgtcagcca taacgctact
     9841 ttatgttggt agcgggtaaa gaccgtgcgg gcgtaatgct cgaagtgatc gatgaccgcc
     9901 cgattagccc aaccgccgta gtttttcacc agcccatata gcatttcgta atgggataac
     9961 gttaccagcg gcttgatccc cgcctgcgcc atttcatcaa acagccgatc gtaaaacgct
    10021 aaccccgctt cattcggttc ggcttcgtcg ccctgaggga aaattcgcgc ccaggcaatg
    10081 gaaatacgca gacaggtgaa gcccatctcg gcaaataacg cgatatcttc cgggtaacgg
    10141 tgataaaaat cgatggcgac atctttgata ttctctttcc ccaggatgcg cggttccatt
    10201 tttcccatta cgccatgagg ctgtaaatct gaggtcgtga tccctttgcc atcttccagc
    10261 caggcacctt ccacctgatt ggcagctgtt gcgccacccc aaagaaatgt ttctggaaat
    10321 gctttcgtaa ttaactcctt ttatcgttag cgaatgatgg ataacagcgg ttcacctgcg
    10381 cttatctgcg ccgtgccgtg gggtaatacg tctgtaaaat catcgctatt actgattaat
    10441 accggcgtcg tcagatcaaa tccggcctcg cgaatagcag ggatatcaaa agaaatcagc
    10501 cgatcgcctg tattgacctt gtcacccacg ttgacgtgag cggaaaagaa tttgccgtcc
    10561 agttttacgg tgtcgatacc gacatgaatc aggatctcca caccatcatc tgactcaatg
    10621 ccaatggcgt gtaatgtggc gaacaacgaa gcaattcgac ccgcaaccgg agaacgcact
    10681 tcaccaaccg agggcagaat ggcgatacct ttacccagca ggccactggc aaacgtggta
    10741 tcagcgacgt gaatgagcgg cacaatctct cccgtcatcg gtgaacagat accgccctgc
    10801 tcaggtggtg taataacctc tggtgttttc tcttgcgggg caccctgcgc tggctgacgt
    10861 ttactggtga tgaaatgaag catcaacgta ccaacaaatg cgcaacctat ggcaatgaca
    10921 ccgccaataa cgctggccca gacggtgaaa tcaatccccg ttgacgggat ggtctgcatg
    10981 aaggtgaaaa tacttggcaa accaaaggag taaactttcg tttgcgcgta gccaatgatg
    11041 gtggccccca atgctccact gatacaggcg ataacaaagg ggtacttacg cggcaggttg
    11101 acgccatata ccgctggttc ggtgatacca aacagacccg tcaacgccgc tgatcccgcc
    11161 accacttttt tctgcgcatc gcgttcgcag aggaagacgc cgagcgccgc cccgacctgc
    11221 gccataatgg cgggcattaa cagcgggatc atggtgtcgt agcccagcac ggtgaagtta
    11281 ttgatacaca gcggcaccag gccccagtgc agtccgaaca tgacgaagat ttgccagaag
    11341 ccgcccatta ccgcgcccgc aaatgcagga accgcctgat aaagccagag ataaccggcg
    11401 gcaatcattt cgcttatcca ggttgatagc ggccccacca gcagaaaggt gacgggtgtg
    11461 ataaccatca gacatagcaa tggtgtgaag aaatttttga ttgccgacgg taaccacgca
    11521 ttaagtcggc gttccagaat gctgcacaac caggcagaaa aaataatggg aataaccgat
    11581 gacgagtaat tcaacaatgt gaccggaata cccaggaaat ccagccccgg cgcatccgct
    11641 ttttgcccgt tctcgaaggc agtcagaatt aatggatgca ctaacgctcc accaatcacc
    11701 atggcagtaa atggattacc gccgaagcgt ttgcccgcgg tgtatcccag gattatcggg
    11761 aagaacccaa acaaggcatc actggcgctg aataaaatta aataagtacc actttgttcg
    11821 gtcgtccact gaaaagtgag cgccagagcc agcatacctt tcaggatccc ggttgccgcc
    11881 atcagaccga tcagaggcgt aaaaatacct gaaataacat aaacaaagcg gtttagcaga
    11941 ttacctttct catcattttc cggtgcctgt tgcgcttttt cgccaaggcc tgccacgctg
    12001 ttaaccgcca ggaagacatc ggccacatgg ttacctatga ccacctgaaa ctgaccaccg
    12061 ctttccacca ccataataat accgggggtc tttttcagta cctcagcttg cgctttgctt
    12121 tcatccttta acttaaaacg taatcgcgtt gcacaatgca tcagactcac aatgttatct
    12181 gcgcccccga ctcctgcgac tatttttctg gctaactccg tcataacttg ccctctaccg
    12241 ctttgcggca aaactccaaa aaaaaacctg aaaaaaacgg cctgacgtga atcaagcaat
    12301 ttttttcagg ttttgcccgc ttagtgcggt aacaatcctt tactcagtaa taatatttca
    12361 gtgttctttg cgcacgcgct ctatatttat ggctaaaaac ataatctctg cgggtgaaat
    12421 tttacgttga tactgcaaac caataaaaat ggcgatccgt tccgcacatt gccatgcttg
    12481 cgggtaattt tgttttactg cttgttgtaa tgattcatca ctatcgttaa ttgaagcatg
    12541 ttcaagaata cgccaggata aaaacttcag atgtgtaacc agtcgctgat aactcaagct
    12601 ttcttcctgg taattaaggc tgaactgaaa ttttattaat tgcagcattt cgcgcattaa
    12661 ctgcgtgaca cctgcaacat cctccatatt tccgctcatt tgggcactga ccagatgcat
    12721 ggcaataaag cccacttcat ctttcggtaa ctgcacgccc aaccgtttat caataatggt
    12781 taacgcttct tcccctagct ggaactcttt cgggtaaagc cgctggatat cccacagcaa
    12841 cgggttgggc agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt
    12901 tagcgagata taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataag
    12961 acgatcacag gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc
    13021 gttcagttca tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc
    13081 gccagcgcgt ttttgaaagc caattccgcg ccccatgacg actttttccc gctgttgatc
    13141 atcaataacc accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag
    13201 gacctttttt ataaacaaaa aaacccgact tcaccagtat tctctggtta tgtcaggttt
    13261 tgcctgcgaa tgcagtaaca atccagtcat ttattaatgg tttttataac gaacatccag
    13321 gttcggaaat taatttaatt gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat
    13381 gaccatgctc gcagttatta actttgtgta attttaggaa tttataaagt tatatataac
    13441 aaat
//
LOCUS       DAJVQH010000113        12910 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJVQH010000113 DAJVQH010000000
VERSION     DAJVQH010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.113"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(506..1597)
                     /gene="ychF"
                     /locus_tag="OQM87_003234"
     CDS             complement(506..1597)
                     /gene="ychF"
                     /locus_tag="OQM87_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCS1509267.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1714..2298)
                     /gene="pth"
                     /locus_tag="OQM87_003235"
     CDS             complement(1714..2298)
                     /gene="pth"
                     /locus_tag="OQM87_003235"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1509268.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2576..2854
                     /gene="ychH"
                     /locus_tag="OQM87_003236"
     CDS             2576..2854
                     /gene="ychH"
                     /locus_tag="OQM87_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCS1509269.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2909..4588)
                     /gene="dauA"
                     /locus_tag="OQM87_003237"
     CDS             complement(2909..4588)
                     /gene="dauA"
                     /locus_tag="OQM87_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCS1509270.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4713..5660)
                     /gene="prs"
                     /locus_tag="OQM87_003238"
     CDS             complement(4713..5660)
                     /gene="prs"
                     /locus_tag="OQM87_003238"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCS1509271.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5811..6662)
                     /gene="ispE"
                     /locus_tag="OQM87_003239"
     CDS             complement(5811..6662)
                     /gene="ispE"
                     /locus_tag="OQM87_003239"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCS1509272.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6662..7285)
                     /gene="lolB"
                     /locus_tag="OQM87_003240"
     CDS             complement(6662..7285)
                     /gene="lolB"
                     /locus_tag="OQM87_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCS1509273.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPTNMELTDGGQRIKLKMDNWIVK"
     gene            7499..8755
                     /gene="hemA"
                     /locus_tag="OQM87_003241"
     CDS             7499..8755
                     /gene="hemA"
                     /locus_tag="OQM87_003241"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCS1509274.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8797..9879
                     /gene="prfA"
                     /locus_tag="OQM87_003242"
     CDS             8797..9879
                     /gene="prfA"
                     /locus_tag="OQM87_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCS1509275.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9879..10712
                     /gene="prmC"
                     /locus_tag="OQM87_003243"
     CDS             9879..10712
                     /gene="prmC"
                     /locus_tag="OQM87_003243"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1509276.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10709..11101
                     /locus_tag="OQM87_003244"
     CDS             10709..11101
                     /locus_tag="OQM87_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCS1509277.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11105..11914
                     /locus_tag="OQM87_003245"
     CDS             11105..11914
                     /locus_tag="OQM87_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCS1509278.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11950..12804
                     /gene="kdsA"
                     /locus_tag="OQM87_003246"
     CDS             11950..12804
                     /gene="kdsA"
                     /locus_tag="OQM87_003246"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCS1509279.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3229 a         3243 c         3343 g         3095 t
ORIGIN      
        1 cgcttgatgt atctgcataa caaaaacaat atgcgccatt tttgcattac taataagaaa
       61 aagcaaaaaa catcgatatt catttttggt caatggattt tctggcctat cgttattatt
      121 gtgctactgg ttatcatttt catcttgcca ggatcgcacg taatcacgtt attcggggac
      181 tttatctgcg ggatattacc cttcaggtaa gtccttttct cgccagcata ttggagcgag
      241 atcaatattg atcagctgtg aagagggctg atgtgtagtg gaacaaaaac tgacgttata
      301 tcactgctaa tgagatatca gtcatctgaa cacttatgtc tttaattatt ctcgtggttc
      361 actgtaggca ataagcacaa aagtgtagga tgttacaaga atgattagga ctcggtgaaa
      421 tgaaaaatcc acgcaattgc gtggatttta tatacttttg cgctcttcat gagatttagc
      481 gaaacctcat gagacaataa attaattaga cgttgaaaag gaagttcatc acatcgccat
      541 ctttcacgat gtaatcttta ccttctgcac gcattttgcc tgcttctttc gcgccttgtt
      601 cacctttgta agtgatgaaa tcttcaaacg agatggtttg tgcacggata aagccttttt
      661 caaaatcagt atggattttg cccgctgcct gcggcgcggt tgctccaacc ggaatggtcc
      721 atgcacgcac ttctttcacc ccagcggtga agtaagtttg caggttcagc agcttataac
      781 cggcacggat cacacggttc aggcccggtt cttccagccc aagctcctgc ataaactcgt
      841 cacgttcttc gtcgtccagt tcggcaatgt ctgcttcaac agcagcacaa accggaacca
      901 caacagaacc ttctttcgcc gcgatttcac gcacctggtc aagatatggg ttgttttcaa
      961 aaccgtcttc gttgacgttg gctatgtaca ttgttggttt cagcgtcagg aagctcaggt
     1021 aacgaatagc cgctttctct tcagcgctta aatccagcgc gcgcagcata cctgcgtttt
     1081 ccagctgggg caggcatttt tccaggaccg ccagctcagc tttcgcgtct ttatcgccac
     1141 ctttggcttt cttctgtacg cgatgaatcg cacgttcgca ggtgtcgagg tctgccagcg
     1201 ccagttcggt gttgataact tcaatatcgt cagccgggtt aactttgccg gaaacgtgaa
     1261 tgatgttgtc attttcaaag cagcgaacaa cgtgaccgat cgcttcggtt tcacggatgt
     1321 tggtcaggaa ctggttaccc agaccttcgc ctttcgatgc gccttttacc agaccggcga
     1381 tatcgacaaa ttccatggtc gtgggaagcg tacgttgcgg ttttacgatt tcagccagtt
     1441 gatccaggcg agggtcaggc attggtacga cgcctgtgtt cggctcaatg gtgcagaatg
     1501 gaaagttggc cgcttcaata ccggctttgg tcagcgcgtt gaacagggta gatttcccga
     1561 cgttgggcaa accgacgata ccgcatttga atcccatgat ttaaatcacc ttaatatctt
     1621 aataatcaac ctgttattgc taacagattg cagaaatgga aataactttg cctattatac
     1681 acggcactcg gcaaaaatgc cgcagacaac gacttattgc gctttaaagg cgtgcaatcg
     1741 gttcgttgct ttggtcaagc catctgtaaa ccacatttca gtacaacgcg ccgcttcgtc
     1801 gatggcttca tcaattaact tctgttcact aacaggcggt ttgcctaaca caaaaccgac
     1861 aactttattt ttatcgcccg gatgaccgat tccgatgcgt aaacggtgaa agttagggtt
     1921 attacccaat ttactgatga tgtctttcag tccattgtga ccaccatggc caccgcccaa
     1981 cttaaatttg gcgacgccag gaggcagatc cagttcgtcg tgggccacca gaatttcgtc
     2041 cggattaatg cggaaaaaac tggccatcgc cgcaacggct ttgccgctga gattcataaa
     2101 tgtagtcggg actaacaggc ggacatcttc gcctccaagg gtgactcgcg aagtataacc
     2161 aaagaattta gcctcttcgc gcagcggagc gcgcaaacgc tctgccagta agtcaacgaa
     2221 ccaggcacca gcattatgtc gcgttgcggc gtattcagca ccggggttcg ccaggccgac
     2281 aatcaattta atcgtcacgt ttttttgtcc tgagtgtgta cataactggc gcgtagttta
     2341 ctggttgcgg ccccgcttga caaaaaactg cgtatcaaat gcagataacg taataattgc
     2401 ctgagtggac tattagaaag tcaaggtgtt caggcgttta tttgtaaagt tttgttgaaa
     2461 taagggttgt aattgtgatt acgcccgcac ataacccaca gggtgttgtc tatactttac
     2521 acataaggaa gaggggtatt ccctgttaca acccagaaag ttccggaggt gacatatgaa
     2581 acgcaaaaac gcttcgatac tcggtaacgt gctcatgggg ttgggtctgg tggtaatggt
     2641 ggtcggcgtg gggtattcaa tcctcaacca gttaccacag tttaatatgc cccagtattt
     2701 cgcacatggt gcagtgctaa gtattttcgt cggtgccatt ctctggctgg cgggtgcccg
     2761 tgttggcggg catgaacagg tgtgcgaccg ttactggtgg gttcgtcact atgacaaacg
     2821 ttgccgccgt agcgataatc gccgtcatag ctaacaaata atgcagtttg ctgaccagtc
     2881 agtttgcgct gactggtcaa tctcgtactt ataaatccgc catcgccgcg cgacgattcg
     2941 ggaagaacgc gaggcgtccc gggatcggtt gaatgcctgc gcgagccata gttcgcagtg
     3001 gctggaattc cacgttgcac acgcgcagtt cacatccttc gggcagacgc ttcacaaaac
     3061 gctggaacgc atcaagacca ccagcatcaa gtaccggaac ggcatcccac ttcagaatca
     3121 caatccgttt gccttcaaga cgtgactcca ggtccgtgaa taagccttca gcagcagcaa
     3181 aaaacagcgg gccaataacg cgcagaacca ggacatcgtc tggaacatct acgactaccg
     3241 gtgccaggcg agtcatacgt gcgatacgac gcataaacag cagcgatgcc agcacgatcc
     3301 ccacgctgat ggcaataacc atatcaaaca gcacggtcag tgacatacac agcagcatga
     3361 cgatgatgtc atctttcggc gcatgacgca gcaagtcgac cactttgtgc gcttcactca
     3421 tgttccacgc caccatcaac agcagggctg ccatggcgga aagcggcagc caggagagca
     3481 gtggtgccag taccagcagg gcgagaataa ccagaataga gtggatcacc gccgagatag
     3541 gggaagttgc cccggcacgg acgttagcgg cagaacgcgc gatggcagca gtagcggtaa
     3601 taccaccaaa gaatggagcg atgatattcc ccagcccctg accgaccagt tcactattcg
     3661 ccttgtgctt agtcccggtc ataccatcca gtaccacagc gcagagcaga gattcgatcg
     3721 ccccgagcat tgccattgag aatgccgcag gcagcagggt gcgaatagaa tcccaggtta
     3781 gcgtgaattc tgaattaggc agatcccacg gcagcaccag ttgcggcagc agttgcggaa
     3841 taccgttacc ctgagaacca tcggccagga cgtagtggaa ttgcgaaccg atggtagcaa
     3901 catgtccgcc gagcaggtta acaatcccca tcaccgcgca accagccagc aaggccggaa
     3961 ggtgaccggg taaacgaatg cccagacgcg gccagaaaac cagaatgcca agcgtcacaa
     4021 tgccaatggc agcatcaccc acattaatgg tcggcagcgc cataaataat gcgccgactt
     4081 tttgtagata atgttccggg acatgggcca tttgcagacc gagaaaatct ttaatctgca
     4141 tggtaccgat ggtgatcccg atacccgagg tgaaacctaa ggtgacggaa accggaatat
     4201 actcaatcag acgaccaaag cgtgccagcc ccatcagaat caaaaagatt cccgacaaca
     4261 aggtcgcaac cagcagtcct gccagtccaa actgttgcga aacgggatag agaattacca
     4321 caaatgccgc agtcggaccg gaaacgctaa agcgtgaccc acccgttaga gcaatgacaa
     4381 tccccgcaac agctgcggta tataaaccgt actggggtgc cacaccacta ccaatagcca
     4441 acgccatcgc cagcgggata gcaataatcc cgacggttat cccggcaatc aggtcacggg
     4501 taaaccgtgc ggcagtatat ttttctttcc agcaagcgtc gatcagagcg cggaaaggca
     4561 tcacatgtga ggaaaatatt ttgttcacaa taatgtttca tccgtgagcg catcatctgt
     4621 caactaaatg gcaggtgaag gaggcatagg tcatacaaat ggatattaca gacaaaaaaa
     4681 cccgccgcag cgggtctttg agccgggttc gattagtgtt cgaacatggc agagatcgat
     4741 tcttcgttgc tgatacgacg aatcgcttcg gccagcatac ctgacagggt cagagtacgc
     4801 acgttcggca gtgatttgat ttcatcgctc agcggaatgg tatcgcagac aacgacttca
     4861 tcaattacag agttacgcag gttgttcgcc gcgttgccag agaagatcgg gtgagtcgcg
     4921 tacgcaaata cacgtttagc accacgttct ttcagcgctt cagcagcttt acacagcgta
     4981 ccgccagtgt cgatcatatc atcgaccagt acgcagtcac gacctgcaac gtcaccgatg
     5041 atatgcatca cctgggaaac gttcgcacgc ggacgacgtt tgtcgatgat tgccatatcg
     5101 gtatcgttca gcagcttagc gatagcgcgg gcacgcacaa cgccgccgat gtccggagaa
     5161 accacaattg ggttatccag attcagctgc agcatgtctt ccagcaggat cgggctacca
     5221 aatacgttat caaccggaac gtcgaagaaa ccctgaatct gttcagcgtg cagatccact
     5281 gtcagcacac ggtcaacacc gacgctggag aggaagtctg caaccacttt cgcagtgatt
     5341 ggtacacgag cggaacggac gcgacggtcc tggcgcgcat agccaaagta ggggataaca
     5401 gcggtgatac gacctgcgga agcacgacgc agggcatcaa ccataacgac taattccatc
     5461 aggttgtcgt tagtaggggc acaagtggac tggatgatga aaatatcacc accgcgtaca
     5521 ttttcgttaa tttgtacgct gacttcgccg tcgctaaagc gacctacagc ggcgtcgccg
     5581 agtgaagtgt acaggcggtt ggcaatacgt tgtgctagta ccggggtggc gttaccagca
     5641 aaaagcttca tatcaggcac gagaagaacc tcaggcatgc gtccattggt ggaaagaatc
     5701 tgccgaaaac tgtgcgggcc aggcatgatc ctttccaggc ggtgtattaa agagcgcgat
     5761 gcaacgtctg gaacaaggtg acgttgtcac cgaaactcag cttgcccggc ttaaagcatg
     5821 gctctgtgca atggggaaag attaacgcct ttcgccacaa agccattgag ccattccggg
     5881 gcttgctcta gcacctggcg ggcttcagac tctgtatcaa attcagcaaa gacacaggcc
     5941 cctgtcccag tcaggcgcga cggggcgtat tttaacagcc aggaaagcac cgcatcaacc
     6001 tcgcgaaaac gttttcttgc gataacctcg caatcattgc tgaattcaca ttttagcaac
     6061 gtttctattg acctttttgg cgtattgcgc gggagttcag gatctttaaa aatcaccgga
     6121 gtcggaatac ttacaccagg gtgcgccacc agataccact tctctggcgg atccaccggc
     6181 gttagtattt caccaacgcc ttcggcaaac gcggcatgcc cccgaacaaa gacaggaaca
     6241 tctgcgccca gcgtcagccc catttccgcc agctcatcca tgcttagccc gcattgccag
     6301 agatgattta atgccaccag gaccgtcgcg gcattggatg aaccaccgcc gagaccgccg
     6361 cccatcggca aacgcttgtc aatgctgata ttcgcaccgc ttcccgtcgg aagacgcccg
     6421 ctgtctgccg cagttttcat caacaaccgc gctgcgcgaa cgatcaggtt atcttcatgt
     6481 tccacgcctt caacgggcgt taacagacga atatccccat cgtcacgaag ctcaatgctg
     6541 atggtgtcgc cgtaatcaag aaactgaaac agcgtttgca gcgtgtggta accatccgca
     6601 cgctgaccgg taatgtataa aaacagatta agttttgccg gagagggcca ctgtgtccgc
     6661 attatttcac tatccagtta tccattttta acttgatgcg ttgaccaccg tcggtgagtt
     6721 ccatattggt tggcatcgca ggttgcgttt tggtgtcata accaccataa acaaccttcc
     6781 agtttttgcc attctggctg taggtaattt cgctcaggcg gtactggtcg tccagtttgt
     6841 agtcggttgc atcacccggt aaacctaaaa tccactggcg caagctgttg agcggaattg
     6901 gcattccggt caatttgcca atcatctctt cggcgtcatc ggcggtataa cgctgacctt
     6961 tattgtcgac taactgcacg ttacccggtt gagcattcag ctccagttcc gtgctgccca
     7021 atgggttagt gagcagcaga cggtagcgat cctggccggt ttgctgccag aaaaagcggg
     7081 cgtacacttt ttgttggtca gaaatataag cgaacgcgcc gcgagtctga tactgattaa
     7141 gattgcgcac gtcttgctga tgctgacgcc attgtggcga atccgggctt ttgccaggac
     7201 ctttgggcgt ggtaacggaa caggcagtga gcacaagagc agccagcggt agcaggcgga
     7261 taagacgaaa atcgggcagg ggcatagtga tgacaagtcc ttgagatacg ttgcagttat
     7321 aacccttaat gctagcgtta ccgtccgcta tcgtctatgt tcaagttgtc ttaattgcca
     7381 gaatctaacg gctttcggca attactccaa aagggggcgc tctcttttat tgatcttacg
     7441 catcctgtat gatgcaagca gactaaccct atcaacgttg gtattatttc ccgcagacat
     7501 gaccctttta gcactcggta tcaaccataa aacggcacct gtatcgctgc gagaacgtgt
     7561 atcgttttcg ccggataagc tcgatcaggc gcttgacagc ctgcttgcgc agccgatggt
     7621 gcagggcggc gtggtgctgt cgacgtgcaa ccgcacggaa ctttatctta gcgttgaaga
     7681 gcaggataac ctgcaagagg cgttaatccg ctggctttgc gattatcaca atcttaatga
     7741 agaagatctg cgtaaaaacc tctactggca tcaggataac gacgcggtta gccatttaat
     7801 gcgtgttgcc agcggcctgg attcactggt tcttggggag ccgcagatcc tcggtcaggt
     7861 taaaaaagcg tttgccgatt cgcaaaaagg tcatatgaag gccagcgaac tggaacgcat
     7921 gttccagaaa tctttctctg tagcgaaacg cgttcgcact gaaacagata tcggtgccag
     7981 tgctgtgtct gtcgcttttg cggcttgtac gctggcgcgg cagatctttg aatcgctctc
     8041 tacggtcaca gtgttgctgg taggcgcggg cgaaaccatc gagctggtag cgcgtcatct
     8101 gcgcgaacat aaagtacaga agatgattat cgccaaccgc actcgcgaac gtgcccaaat
     8161 tctggcagat gaagttggcg cggaagtgat tgccctgagt gagatcgacg aacgtctgcg
     8221 cgaagccgat atcatcatca gttccaccgc cagcccgtta ccgattatcg ggaaaggcat
     8281 ggtggagcgc gcattaaaaa gtcgtcgtaa ccaaccaatg ctgttggtgg atattgccgt
     8341 tccgcgcgat gttgagccgg aagttggcaa actggcgaat gcttatcttt atagtgtgga
     8401 cgatctgcaa agcatcattt cgcacaacct ggcgcagcgt aaagccgcag cggttgaggc
     8461 ggaaactatt gtcgctcagg aaaccagcga atttatggcg tggctgcgag cacaaagcgc
     8521 cagcgaaacc attcgcgagt atcgcagcca ggcagagcac gttcgcgatg agttaaccgc
     8581 caaagcgtta gcggcccttg agcagggcgg cgacgcgcaa gccattatgc aggatctggc
     8641 atggaaactg actaaccgct tgatccatgc gccaacgaaa tcacttcaac aggccgcccg
     8701 tgacggggat aacgaacgcc tgaatattct gcgcgacagc ctcgggctgg agtagcagta
     8761 catcattttc tttttttaca gggtgcattt acgcctatga agccttctat cgttgccaaa
     8821 ctggaagccc tgcatgaacg ccatgaagaa gttcaggcgt tgctgggtga cgcgcaaact
     8881 atcgccgacc aggaacgttt tcgcgcatta tcacgcgaat atgcgcagtt aagtgatgtt
     8941 tcgcgctgtt ttaccgactg gcaacaggtt caggaagata tcgaaaccgc acagatgatg
     9001 ctcgacgatc ctgaaatgcg tgagatggcg caggatgaac tgcgcgaagc taaagaaaaa
     9061 agcgagcaac tggaacagca attacaggtt ctattactac caaaagatcc tgatgacgaa
     9121 cgtaacgcct tcctcgaagt ccgtgccgga accggcggcg acgaagcagc gctgtttgca
     9181 ggcgatctgt tccgtatgta cagccgttac gccgaagccc gccgctggcg ggtagaaatc
     9241 atgagcgcca gcgagggtga acatggtggt tataaagaga tcatcgccaa aattagcggt
     9301 gatggtgtgt atggtcgtct gaaatttgaa tccggcggtc atcgcgtgca acgtgttcct
     9361 gctacggaat cgcagggtcg tattcatact tctgcttgta ccgttgcggt aatgccagaa
     9421 ctgcctgacg cagaactgcc ggacatcaac ccagcagatt tacgcattga tactttccgc
     9481 tcgtcagggg cgggtggtca gcacgttaac accaccgatt cggcaattcg tattactcac
     9541 ttgccgaccg ggattgttgt tgaatgtcag gacgaacgtt cacaacataa aaacaaagcg
     9601 aaggcgcttt cagtgctcgg tgctcgcatc cacgctgctg aaatggcaaa acgccaacag
     9661 gccgaagcgt ctacccgtcg taacctgctg gggagtggcg atcgcagcga ccgtaaccgt
     9721 acttacaatt tcccgcaggg gcgcgttacc gatcaccgca tcaacctgac gctctaccgc
     9781 ctggatgaag tgatggaagg taagctggat atgctgattg aaccgattat ccaggaacat
     9841 caggccgacc aactggcggc gttgtccgag caggaataat ggaatatcaa cactggttac
     9901 gtgaagcaat aagccaactt caggcgagcg aaagcccgcg gcgtgatgct gaaatcctgc
     9961 tggagcatgt taccggcaga gggcgtactt ttattctcgc ctttggtgaa acgcagctga
    10021 ctgacgaaca atgtcagcaa cttgatgcgc tactgacacg tcgtcgcgat ggtgaaccca
    10081 ttgctcattt aaccggggtg cgagaattct ggtcgttgcc gttatttgtt tctccagcga
    10141 ccttaattcc gcgcccggat acggagtgtc tggtggagca ggcactggcg cggttgcctg
    10201 aacaaccttg ccgtattctc gatctcggga cggggaccgg ggcgattgcg cttgcgctgg
    10261 ctagcgagcg cccggactgc gaaattatcg ctgtagatcg tatgcctgat gctgtctccc
    10321 tggcacaacg taatgcccag catctggcga tcaaaaatat ccacattctg caaagcgact
    10381 ggtttagcgc gctagccggg cagcagtttg cgatgattgt cagcaatccg ccgtatattg
    10441 acgagcagga cccacatctt caacaaggcg atgtccgctt tgagccgctc actgcgctgg
    10501 ttgcggcaga cagtggaatg gcagacatcg tgcatatcat cgaacagtcg cgtaacgcgc
    10561 tggtatccgg cggctttctg cttctggaac atggctggca gcagggcgaa gcggtgcgac
    10621 aagcatttat cctcgcgggg tatcatgacg tcgaaacctg ccgtgactat ggtgataacg
    10681 agcgcgtaac gctcggccgc tattatcaat gacaagtttt tctacactgc ttagtgttca
    10741 tcttattagt atcgcgcttt ctgttgggct attaacctta cgtttctggc tacgttatca
    10801 gaagcatcca caggcatttg ctcgctggac gcgcattgtg ccgccggttg tcgatacggt
    10861 gttattgtta agcggcattg cgttgatggc taaagcgcac atcctgccat tttccgggca
    10921 ggcacagtgg ctgactgaaa agctgtttgg agttatcatt tatatcgttt tgggttttat
    10981 tgcactcgat tatcgtcgta tgcacagtca gcaggcgcgc attattgcct tcccgctggc
    11041 gttggtggtg ctgtacatca tcattaaact cgccaccaca aaagtaccgt tactggggta
    11101 agttatgaga tcgttagctg atttcgaatt taataaagcg ccattgtgcg aaggcatgat
    11161 cctggcttgc gaagcaatcc gccgcgattt tccctcgcaa gatgtttacg acgaactgga
    11221 gcgtctcgtt agtctggcga aggaagaaat cagccagctt ctgcctttag aagagcagct
    11281 ggaaaaactg atcgcgctgt tttacggcga ctggggattt aaagcctcac gcggcgttta
    11341 tcgtctttcc gatgcattat ggctggacca ggtgttaaag aatcgacagg gcagtgcggt
    11401 atcattaggt gcggttttat tatgggtcgc gaatcgtctc gatttgccgc tgctgccggt
    11461 gattttccct acgcagctga tattgcgcat tgaatgtccg gatggcgaaa tttggctgat
    11521 taatcctttt aacggtgaat cgttaagcga acatatgctg gacgtatggt taaagggaaa
    11581 tatcagcccg tcggcggaac tgttttatga agaccttgat gaagctgata acattgaggt
    11641 aatccgcaaa ttgctggata cactcaaagc ctcgttgatg gaagaaaatc agatggagct
    11701 ggcgttacgc accagcgaag ctttattaca attcaaccct gaagatccct atgaaattcg
    11761 cgatcgcggg ttgatttatg cgcaactgga ttgcgaacac gttgcgttga acgatttaag
    11821 ttatttcgtt gaacagtgtc cggaagaccc gatcagcgaa atgatccgtg cgcaaataaa
    11881 taacatcgcg cataaacata ttgtgctgca ttaattaatc gacattttac tcaagattaa
    11941 ggcgatccta tgaaacaaaa agtggttagc attggcgaca tcaacgtagc aaatgacctg
    12001 ccgttcgtac tgtttggcgg tatgaacgtg ctggaatctc gcgatctggc gatgcgcatt
    12061 tgcgagcact acgtaactgt gacccagaaa ctgggtatcc cttacgtgtt caaagcctct
    12121 tttgacaaag ccaaccgctc ctccatccac tcttatcgtg gaccgggcct ggaagaaggg
    12181 atgaaaatct tccaggagtt gaagcagact tttggcgtga aaattatcac cgacgttcac
    12241 gaaccaagtc aggcacagcc cgttgctgat gtcgtggatg tgattcagtt gccggcgttt
    12301 cttgctcgcc agactgacct ggttgaagcc atggcgaaaa ccggtgcggt aattaacgtc
    12361 aagaaaccac agtttgtcag cccggggcag atgggtaata tcgttgataa attcaaagaa
    12421 ggcggcaacg aaaaagtgat tctttgcgat cgtggtgcta acttcggcta tgacaacctg
    12481 gttgtcgata tgctgggctt cagcattatg aagaaagtgt ctggtaactc gccggtgatt
    12541 ttcgacgtga cccacgcact gcaatgccgc gatccgtttg gcgcagcttc cggtggtcgt
    12601 cgtgctcagg tggctgagct ggcacgagcc ggtatggcgg taggtctggc ggggctgttt
    12661 attgaagcgc atccggatcc ggaacatgcg aaatgtgatg gtccatccgc gctgccgctg
    12721 gctaaactgg aaccgttcct caagcagatg aaagcgattg atgatctggt gaaaggtttc
    12781 gaagaactgg ataccagcaa gtaatctttt ttgcttgaaa aataaagtct tagcgttctg
    12841 cgttaagact tttttcatgg gtgccagata caaaaaaggc cgcaggctgt tacccctgcg
    12901 gccggtttcg
//
LOCUS       DAJVQH010000114        12860 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJVQH010000114 DAJVQH010000000
VERSION     DAJVQH010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.114"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..393)
                     /gene="yafO"
                     /locus_tag="OQM87_003247"
     CDS             complement(<1..393)
                     /gene="yafO"
                     /locus_tag="OQM87_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCS1509280.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMR"
     gene            complement(396..689)
                     /gene="yafN"
                     /locus_tag="OQM87_003248"
     CDS             complement(396..689)
                     /gene="yafN"
                     /locus_tag="OQM87_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCS1509281.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            complement(741..1796)
                     /gene="dinB"
                     /locus_tag="OQM87_003249"
     CDS             complement(741..1796)
                     /gene="dinB"
                     /locus_tag="OQM87_003249"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCS1509282.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(1867..2652)
                     /gene="lafU"
                     /locus_tag="OQM87_003250"
     CDS             complement(1867..2652)
                     /gene="lafU"
                     /locus_tag="OQM87_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCS1509283.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            2624..4336
                     /locus_tag="OQM87_003251"
                     /pseudo
     CDS             2624..4336
                     /locus_tag="OQM87_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4553..5050)
                     /locus_tag="OQM87_003252"
     CDS             complement(4553..5050)
                     /locus_tag="OQM87_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1509284.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTIQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(5226..5747)
                     /locus_tag="OQM87_003253"
                     /pseudo
     CDS             complement(5226..5747)
                     /locus_tag="OQM87_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            6131..6871
                     /locus_tag="OQM87_003254"
     CDS             6131..6871
                     /locus_tag="OQM87_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCS1509285.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6842..7609)
                     /locus_tag="OQM87_003255"
     CDS             complement(6842..7609)
                     /locus_tag="OQM87_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCS1509286.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(7815..8393)
                     /gene="lpcA"
                     /locus_tag="OQM87_003256"
     CDS             complement(7815..8393)
                     /gene="lpcA"
                     /locus_tag="OQM87_003256"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCS1509287.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            8633..11077
                     /gene="fadE"
                     /locus_tag="OQM87_003257"
     CDS             8633..11077
                     /gene="fadE"
                     /locus_tag="OQM87_003257"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCS1509288.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDTMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(11120..11566)
                     /gene="ivy"
                     /locus_tag="OQM87_003258"
     CDS             complement(11120..11566)
                     /gene="ivy"
                     /locus_tag="OQM87_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCS1509289.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            11747..12517
                     /gene="yafV"
                     /locus_tag="OQM87_003259"
     CDS             11747..12517
                     /gene="yafV"
                     /locus_tag="OQM87_003259"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCS1509290.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            12626..12858
                     /locus_tag="OQM87_003260"
                     /pseudo
     CDS             12626..12858
                     /locus_tag="OQM87_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
BASE COUNT         3044 a         3330 c         3411 g         3075 t
ORIGIN      
        1 acgcatgcga aacgcttctg ccatttttcc aattttatgc atttggttgt tatctcgagc
       61 cagtttatga ggttcaggct tcagaatggc aatgagcaac catgcttgct catcaaacgc
      121 accctgacaa tataccaaat gcgcttcgtc attcgttctg ctgaattggc gcaactgtgg
      181 cggaaatgga ttattcacat ttgccagatg aatatgagca actcgctcaa atttgattaa
      241 tggccaggta aacgagtcgt cgtagagtgc atcgcgacca aatatatctg gcaaaacacc
      301 gtcacgctta taggaaataa aatccgccgt taacgcatca agttcctctg ctgtaagttg
      361 caggcgaata agttttgttt tgaatacccg catccttatt ccttaaagtc attgaaatca
      421 tcatccgtca tatcattaag atattgttta gcgcggcgtg taatttcctc taatcttgca
      481 gcacttggac gaaagcgcgc ctttatgggc tttttctcct cttgttcagc aagcatgtcc
      541 attaacgctt cgaatgcgct ggcacttaag agatatcctg cggggcgatt attagaaaga
      601 accgcaactg gttgatcaat aaagtattta gctgggtttt tacgtaactc agtgatattg
      661 accgattttt cagcgagaat ccgatgcata cagtgatacc ctcataataa tgcacaccag
      721 aatatacata atagtataca tcataatccc agcaccagtt gtctttccat ttgcgggtca
      781 agcaacgtca catgcagccc caccagacgc acaccgcgcc cgccgcggcg ttcatcccag
      841 gttttacgcg cggtggcgat taagtcagct ttattcagcc gcggccagac gtgctcctgg
      901 gtggtctgct gaaaatcgtc gaactttaat ttcaccccct ggcgagcaat cagtaaatca
      961 ggttttacct ttgccagacg gcgttcaagt tccggataca gccgctcgat aatcgcttca
     1021 cattcagacc agtgatgaat atcttccgcc atcgtgcgtt ccacgccgac ggattttcgt
     1081 aaccgttcgc tgttaacgtc gcgctcgtca atcccctgac tacgctccca caaaatgcgg
     1141 ccaaatttgc caaagcgttt aagcagaatc accagatcac acttttgtac atcaccgcag
     1201 gtccgcagcc ccatcgcttc cagttttgcc gccgagactt tgccgacgcc ggggattttt
     1261 tccagtggta aggtttgtaa aaatgccgga acttctgccg gcgtaatcac aaactgaccg
     1321 ttgggtttat tcatgtcgga ggcgattttg gcgagaaact ttactggtgc cacgcccgca
     1381 gatgccgtca gttgcagctc gttgaagatc gtctggcgga tttcctgggc gatgagggtc
     1441 gcagaaccgt ggcaatggac gctatcggta acatcgagat aagcctcatc cagtgacaac
     1501 ggttcaatgc gcgaggtgta gcgcgagaag atttcacgga tatgatttga ggcttctttg
     1561 taggcgtcaa agcgccccgg aagcaaggtg agatgtgggc ataatttgag cgccatccca
     1621 gtaggcatag cgctacgtac gccaaattta cgcgcgggat aattggcggt gctgatcacc
     1681 ccccgacgtt cgcggctgcc gccaatagca atagggatat cgcgcagagc gggattgtcg
     1741 cgcatctcca ccgctgcgaa aaagcagtcc atatccacat gaatgatttt acgcattgct
     1801 cacctctcaa cactggtaaa gtatacagtg atttcaggtt ttgagaaatg cgtaaagatt
     1861 cagcatttag cgcattcgct gcgaaagcac ctgctgctta tccagctttt gcaccagcgg
     1921 ctgcaccact ttatccccat gctgaccaaa gtattgatac agcgtatcgg acgcactttt
     1981 ggtcagtacc ataatctcaa tacgccgatt gcccgcgctt tgcggattct tcgcgtccag
     2041 cagcatctgg tccgccattg cgcttacctg catcacttta tcttccggca ttccggcctc
     2101 ttccagcacc cgacgagctg aaagcgcgcg atcgcccgaa aggttccagt tgttgtagat
     2161 attgtttttg taggccatcg catcggtatg cccggtaata ataattttgt tatagagcga
     2221 gtcgaacact ggcgcgagct ccaccagcaa agttttaaag aacggcataa tctgcgcgct
     2281 gccgcgttcg aacatattgc gattctggtc gtctttaatc agcacgcgta atccctgggg
     2341 aacaatctcc atctccagat tcgcttccat atgcgcatca cgggcaatgg tgttgatact
     2401 ggttgccaac tcgccaagct cggtggcgga tttcttcttc agcagcgccg ttttctcatt
     2461 cacatcacgc gcttttttct ccgtctcttc cggcaccgcc acggtggcag gttttggcgg
     2521 atgagacggg ctgattttat ttaacggcga cagcccacca ccgttaaaaa tcgactgccc
     2581 gtgcagcgcg gcgataatgc tttcacgctc ggatttactg acgctgttga caatcaactt
     2641 tatcgtcgtc accaaagagg ccgagcgtat ttccgaggtt tctgcccgct ttaccctgga
     2701 cgcgatgccc ggcaaacaga tggcgattga cgccgatctt aacgccggat tgatcaacca
     2761 ggcgcaggcg caaacccggc gtaaagatgt tgccagcgag gccgatttct acggcgcgat
     2821 ggacggggca tcgaagtttg tacgcggaga cgccatcgcc gggatgatga ttctggcgat
     2881 caacctgagc ggcggcgtct gtatcggcat cttcaaatac aacctgagcg ccgacgccgc
     2941 cttccagcaa tacgtgctga tgaccatcgg cgacggcctg gtggcgcaga tcccttccct
     3001 gctgctctcc accgcggcgg cgattatcgt cacccgcgtc agcgacaacg gcgatatcgc
     3061 ccatgacgtg cgccaccagt tgctggcaag cccgtcggtg ctctacaccg ccaccgggat
     3121 catgttcgtg ctggcggtgg tgccggggat gccgcatctg ccgtttttgc tgttcagcgc
     3181 cctgcttggc tttaccggct ggcggatgag caaacgcccg caggcggcgg aggcggaaga
     3241 gaaaagcctc gaaacgctga cccgcaccat cactgaaacc agcgaacaac aggtcagttg
     3301 ggaaactatt ccgctgatcg agcccatcag cttaagcctc ggctacaagc tggtggcgct
     3361 ggtggacaaa gcgcagggca acccgctcac ccagcggatt cgcggcgttc ggcaggtgat
     3421 ttccgacggt aacggcgtgc tgctgccgga gatccgcatt cgggaaaact tccgcctcaa
     3481 gcccagccag tacgctattt tcatcaacgg cattaaggct gatgaagcgg atattccggc
     3541 ggataaactg atggcgctgc cctccagcga aacctacggc gagattgacg gcgtgctggg
     3601 gaacgacccg gcgtacggga taccggtcac ctggatccag cctgcgcaga aagcgaaggc
     3661 tctgaatatg gggtatcagg tgatcgacag cgccagtgtg attgccaccc atgtgaacaa
     3721 gattgtgcgc agctatattc ctgatttgtt taactatgat gacatcacgc agttgcataa
     3781 ccgtttgtcg tcgatggcac cgcgcctggc ggaggattta agcgcggcgc tcaattacag
     3841 ccaattgctg aaagtgtacc gggcgctgct gaccgaaggc gtttccctgc gcgatatcgt
     3901 caccatcgcc accgtgctgg tcgccagtag cacggtgact aaagatcata ttctgctggc
     3961 ggccgatgtg cgcctggcgc tgcggcgcag cattactcat ccgttcgttc gcaagcagga
     4021 gctgacggtg tatacgctga ataatgagct ggaaaatctg ctgaccaacg tagtgaatca
     4081 ggcgcaacag gccggcaaag tgatgctcga cagcgtgccg gttgacccga atatgctcaa
     4141 ccagttccag agcacgatgc cacaggtgaa agagcagatg aaagcggcgg ggaaagatcc
     4201 ggtgctgctg gtgccgccgc agctacgccc tttgctggcg cgttatgcaa ggttgtttgc
     4261 gccggggctg catgtgctgt cgtataacga agtgccggat gagctggagt tgaagattat
     4321 gggggcgttg atgtaagaat ccggcatcgg tgcacaattg ccggatgcgg cgtaaacgcc
     4381 ttatccggcc taccgttcag gcacaagttt ttaggcatga taagacgcgc cagcgtcgca
     4441 tcaggcatct gcgcataact gccggatgcg gcgtgaacgc cttatccggc ctaccgttaa
     4501 agcacgattt gtaggccgga taagcgtaag cgcatccggc accagcgcac agttataaaa
     4561 tgatacgctc cccggcactt aaatccgtta tgtctcccgc ccaatcgatg ggatataacc
     4621 ctcgcgcgac atcgcgatgg aacgttgaga atggccaatc actcacctgc tttacccagc
     4681 catgtttctc tggatttata taaatataat caacatgatg gcgataatct tttgtattgc
     4741 gaatggcatg ctcccaaaaa cgcggttgcc agatattttt caatccgcaa gcatgggtga
     4801 actgcttttt aatctcccgc cagcgagagg aaaaatcatc atcgctttcg ggtaatgtcc
     4861 atatgcaatg catatgctcc ggcaaaacga cccaggcgtt aatttcaaaa ggtctgtctc
     4921 gcttaacgtt aataatggcg ttacggagcg tctggaactg gatggtcaga agatggcttc
     4981 gacgattgcg taaatttacc gtgaaaaacc atgttccccc cttgatgtaa taacgacgat
     5041 attccgacat agcgactcct ttttctcaac ggtaagagga aaaggaaagg ccgacaatga
     5101 cgctgtgtta tatcgggaaa gcggctcgca aaaacgcaca gatttgtagg catgataaga
     5161 cgcgtcagcg tcgcatcagg catccgtgca caaccgccgg atgcggcgta aacgccttat
     5221 ccggcctaca aaatcatctc ttccgtcaaa atcctgcgtg cgcctaaaaa atggtcctgc
     5281 cagaaaggtt cggctaaccg gcttacccga atggtttccc cggtgcgcag tgactcaata
     5341 aactgaccgt cgcccagata cacccccata tggtcggcaa tctcgcggct gtggatatgg
     5401 aaaaatagca aatctccccg gcgcaggtcg ttattcgcca caatcgttgc ccgacggtag
     5461 tggtacatct cattagccgt gcgcgggagt ttcgcctcaa ggatcttgtt gtaggcataa
     5521 aaaactaacc cactacaatc gaagccttga tcgggccgcg tgccgcccca gacgtaaggc
     5581 ttgccaagct gctgctccag gcggtgaatc gccacttcgg tgatgttatg caggtgatcg
     5641 ctgcttaaaa agtggttgtt ttccgccagc tcctgccagc gtctgtcact cgccttgagc
     5701 ggcgcaggaa accattctgg atgctgtttt atctgctccc ggttgtggtg gcgctggcga
     5761 gtaaagggag caaaacgcat aacgaaaaca cgcctggatg caaaaatctg ccgagcaaga
     5821 acgaagacat gaaagacata aaccggctga atgaaggtga gaattttaaa agaccggagg
     5881 agtaaacggg attattaatt gcttatttgc atattactat tgagatttca taatattagg
     5941 aaataaatga tacatgcgct tttcggacta ttgaaaactg cccctgtgct aaatttgtaa
     6001 acccagcgcc gacaggcttg cgtaaaactc ttttggtgat aatgtgagcc ttccaaatcc
     6061 agactattcc gatttgggag ccattttgcc gtcctggcgt gtgtaaccgt tttatcaagg
     6121 aataagcagt atgcgtaaaa tcgcattaat tcttgcgatg ttgttgatcc cgtgcgtttc
     6181 gtttgccggt ttgctgggca gcagtagctc gaccacgcca gtgagcaaag agtataagca
     6241 gcagttgatg ggatcccctg tctacatcca gatcttcaag gaagaacgta cgctcgatct
     6301 ttacgtcaaa atgggcgagc aatatcaact gctcgacagt tataaaatct gtaaatattc
     6361 cggcggctta gggccaaaac agcgtcaggg cgatttcaaa agcccggaag ggttttatag
     6421 cgtccagcgt aatcagttaa aaccagacag ccgttactac aaagcgatta atattggatt
     6481 ccccaatgct tatgaccgtg cgcatggtta tgaagggaaa tacctaatga tccacggcga
     6541 ttgtgtatcc atcggctgct acgcaatgac caatcagggt attgatgaga tcttccagtt
     6601 cgttactggt gcgctggtgt ttggtcagcc gagcgtgcag gtgagtattt acccattccg
     6661 catgaccgac gccaatatga agcgccataa atattccaac tttaaggact tctgggagca
     6721 gctgaagccg ggctacgact actttgagca aacccgtaag ccaccgaccg tttctgtggc
     6781 caatggccgt tacgtggtca gcaagccgtt aagccacgaa gtggtgcagc cacaactggc
     6841 atcaaactac acgctccccg aggcaaaata agcgccattc gcctggcata attttttgcc
     6901 aggtttcatt gcccgtcagc ggctgtgtcg caatcaccgt gaccacatca ttcggtgtgg
     6961 tctgcgagct gaagtcgatt tccacatcct gatccagcaa cgttgccacg ccaaacggcg
     7021 cgcggcgggt gatccagtgt aaattagtcg agcaatacgc cattacatag cgcccgtccg
     7081 aaagcagcat gttgaaaacg cccttcgccc gcagttcatc cgccagtgag gcgatatatt
     7141 taaatactgc cgccatgttg cccggcgtgc gcgggtaacg ctgcgttaat ttatgcagca
     7201 gccagcaaaa ggctttttcg ctgtcggttt caccgaccgg gcggaagttg ccggtttcca
     7261 gtgatttgta gcccgtcagt tgtccgttat gggcataagt ccagttacgc ccccataact
     7321 cgcgggtaaa tgggtgagta ttttccagcg ccacctcgcc ccgattagcc tggcgaatat
     7381 gagccaccac cgaacaggat tttatcgggt agtcctggac aagtttggcg atgggggaat
     7441 taaagctggg ttgtggatct ttaaatgtgc gacagccttt accttcgtaa aaggtaatgc
     7501 cccagccatc tttatgtggc ccggttccac caccacgctg tacaagtccg gtgaaactaa
     7561 agcagatatc ggtagggacg ttggcgctca tcccgagcaa ttcgcacatg ggtaacctcc
     7621 acaacagcgg gagcgcatgg accccggcga agattgattt aagaattttc aggtcggatg
     7681 cggcgtaaac gaattattcg gcctacggtt cgggtacaga tttgtaggca tgataagacg
     7741 cgtcagcatc gcatcaggca tcagcgcaca agtgccggat gcggcgtaaa cgccttatcc
     7801 ggcctacgcc agacttactt aaccatctct ttttcaatta actggatcag gatatggatc
     7861 actttaatgt gaatctcctg aatgcggtcg gcataaccaa agtgcggaac gcgaatttcg
     7921 atatccgccg tgccagccat tttaccgccg tctttaccgg tcagggtgat cactttcatc
     7981 cccttctcac gcgccgctgc gatcgctttg atcacgtttg cagagttacc ggaggtggag
     8041 atccccagca gtacatcgcc ttcgcgacct accgcttcaa cgtagcggga gaaaatatca
     8101 ttgaaaccga aatcattacc gacgcaggaa atatgactaa cgtcagaaat agcaatcgcc
     8161 gggtagcccg gacggttttc acggtagcga ccggtcaact cttcggcaaa gtgcatagcg
     8221 tcgcaatggg aaccgccgtt gccgcaggaa agcactttgc caccggcttt aaagctgtct
     8281 gctaacagga ccgccgcgcg ctgaatggcg tgaatattgg cgtcatcttt taaaaagtta
     8341 gccagcgttt ccgccgcttc gttcagttcg ttacgaataa gatcctggta catgaggata
     8401 tccttcagca taaatgtaat agacaaaatg cagtgtaccg gataccgcca aaagcgagaa
     8461 gtacgggcag gtgctatgac caggactttt tgacctgaag tgcggataaa aacagcaaca
     8521 atgtgagctt tgttgtaatt atattgtaaa cacattgcta aatgttttta catccactac
     8581 aaccatatca tcacaagtgg tcagacctcc tacaagtaag gggcttttcg ttatgatgat
     8641 tttgagtatt ctcgctacgg ttgtcctgct cggcgcgttg ttctatcacc gcgtgagctt
     8701 atttatcagc agtctgattt tgctcgcctg gacagccgcc ctcggcgttg ctggtctgtg
     8761 gtcggcgtgg gtactggtgc ctctgaccat tatcctcgtg ccatttaact ttgcgcctat
     8821 gcgtaagtcg atgatttccg cgccggtatt tcgcggtttc cgtaaggtga tgccgccgat
     8881 gtcgcgcact gagaaagaag cgattgatgc gggcaccacc tggtgggagg gcgacttgtt
     8941 ccagggcaag ccggactgga aaaagctgca taactatccg cagccgcgcc tgaccgctga
     9001 agagcaagcg tttctcgacg gcccggtaga agaagcctgc cggatggcga atgatttcca
     9061 gattactcat gagctggcgg atctgccgcc ggagttgtgg gcgtacctta aagagcatcg
     9121 cttcttcgcg atgattatca aaaaagagta cggcggactg gagttctcag cttatgccca
     9181 gtctcgtgtg ctgcaaaaac tctctggcgt gagcgggatc ctggcgatta ccgtcggcgt
     9241 gccaaactca ttaggcccgg gcgaactgtt gcaacattac ggcactgacg agcagaaaga
     9301 tcactatctg ccgcgtctgg cgcgtggtca ggagatcccc tgctttgcac tgaccagccc
     9361 ggaagcgggt tccgatgcgg gcgcgattcc ggacaccggg attgtctgca tgggcgaatg
     9421 gcagggccag caggtgctgg ggatgcgtct gacctggaac aaacgctaca ttacgctggc
     9481 accgattgcg accgtgcttg ggctggcgtt taaactctcc gacccggaaa aattactcgg
     9541 cggtgcagaa gatttaggca ttacctgtgc gctgatccca accaccacgc cgggcgtgga
     9601 aattggtcgt cgccacttcc cgctgaacgt accgttccag aacggaccga cgcgcggtaa
     9661 agatgtcttc gtgccgatcg attacataat cggcgggccg aaaatggccg ggcaaggctg
     9721 gcggatgctg gtggagtgcc tctcggtagg ccgcggcatc accctgcctt ccaactcaac
     9781 cggcggcgtg aaatcggtag cgctggcaac gggcgcgtat tctcacattc gccgtcagtt
     9841 caaaatctct atcggtaaga tggaagggat tgaagagccg ctggcgcgta ttgccgggaa
     9901 cgcttatgta atggatgctg cggcatcgct gattacctac ggcattatgc tcggcgaaaa
     9961 acctgccgtg ctgtcggcta tcgttaagta tcactgtacc caccgcgggc agcagtcgat
    10021 tattgatacg atggatatta ccggcggtaa aggcattatg ctcgggcaaa gcaacttcct
    10081 ggcgcgtgct taccagggcg caccgattgc catcaccgtt gaaggggcta acattctgac
    10141 ccgcagcatg atgatcttcg gacaaggagc gattcgttgc catccgtacg tgctggaaga
    10201 gatggaagcg gcgaagaaca atgacgtcaa cgcgttcgat aaactgttgt tcaaacatat
    10261 cggtcacgtc ggtagcaaca aagttcgcag cttctggctg ggcctgacgc gcggtttaac
    10321 cagcagcacg ccaaccggcg atgccactaa acgctactat cagcacctga accgcctgag
    10381 cgccaacctc gccctgcttt ctgatgtctc gatggcactg ctgggcggca gcctgaaacg
    10441 tcgtgagcgt atctcggcac gtctggggga tattttaagc cagctttacc tcgcctctgc
    10501 cgttctgaag cgttatgacg acgaaggccg taatgaagcc gatctgccgc tggtgcactg
    10561 gggcgtacag gatgcgctgt atcaggcaga acaggcgatg gacgatttac tgcaaaactt
    10621 cccgaaccgc gtggttgccg ggctgctgaa tgtggtgatc ttcccgaccg gacgtcatta
    10681 tctggcaccg tctgacaagc tggatcataa agtggcgaag attttacaag tgccgaacgc
    10741 cactcgttca cgtattggtc gcggtcagta cctgacgcca agcgagcata acccggttgg
    10801 tttactggaa gaggcgttgg tggatgtgat tgccgccgac tcgattcatc agcggatctg
    10861 taaagagctg ggtaaaaatc tgccatttac ccgtctggat gaactggcgc acaacgcgct
    10921 gacgaagggg ctgattgata aagatgaagc cgctattctg gtgaaagcgg aagaaagccg
    10981 tctgcgcagt attaacgttg atgactttga tccggaagag ctggcgacga agccggtaaa
    11041 gttgccggag aaagtgcgga aagttgaagc cgcgtaattg aataacggag ccgaaaggct
    11101 ccgtttcttt atccgctaat tatttaaaat taaagccatc cggatggttt tccaggctgc
    11161 cggtcaacgc cgcgaacagc accgttttac catcaatcga aagcgcatcg ttcacattca
    11221 gccaggtgag tttctcttgc gacgttttct catcaatagc cgagaacagc cccgtcatct
    11281 gattagattt ctcggaccac atcacagcga tacgttgcga gccacagtca tgcggtttgc
    11341 acgcgctcat cacctgatac gtctcatctc ccaacgttac ggtttgtgcg ggagtataag
    11401 taccgccttt catcacccat gcaggtagct tatgcccttg caccatctga ttaaacgcag
    11461 ctttggtggt ttcgcccttt gcaaggctgc taatggttaa atcatcctgc gccattgcac
    11521 tggtggcgat gaccagtgcg gcgactgtcg ttattgcctt aaacatcatt cctcccgagc
    11581 ttatcctgcc catgttatta acctcctaaa gcctggcgat aacacgataa tttgcaaatc
    11641 agatatttcc aatatcagca tttgttatca atttattctg cgctaaggca actgcctgat
    11701 aaggtgttaa agttataaaa cccttcgcat aaaggagttg tacaccgtgc ctagtttgaa
    11761 gattacgctt ttgcagcaac cgttggtgtg ggtggatggt cctgccaacc tgcgccattt
    11821 tgatcgtcaa ctggaaggta ttaccgggcg cgatgtgatc gttctaccgg agatgtttac
    11881 cagcggcttt gccatggaag cggcagcttc gtcgcttgca caagatgacg tagtgaactg
    11941 gatgacagct aaggcgcagc agtgcaatgc gctgattgca ggcagtgttg cattacaaac
    12001 ggagtctggt tcggttaacc gctttttgct ggttgagccg ggcggcacgg tacattttta
    12061 tgataagcgt catctgttcc gcatggcaga tgagcatctg cattataaag cgggcaatgc
    12121 gcgagtgatt gtggaatggc gcggctggcg tattttgccg ctggtgtgct acgacttacg
    12181 ttttccagtg tggtcgcgca atctcaacga ttatgacctc gccctgtacg tcgccaactg
    12241 gccagctccg cgctctctgc actggcaggc attgctgacg gcccgcgcga ttgagaatca
    12301 ggcgtatgtg gcgggatgca atcgcgtcgg cagcgatggc aacggctgcc attatcgcgg
    12361 tgacagccgg gtgattaatc cgcaaggcga gattatcgct accgccgacg cgcataaggc
    12421 aacgcgtatt gatgcggagc tgtcgatggt ggcattgcgg gaatatagag aaaagtttcc
    12481 ggcatggcgg gatgcggatg agtttaggtt gtggtgagtg ggggttgtgt ttagggccgg
    12541 ggctgggcta gattacgaaa gcccctaact taaacacaat attacgccag aagtcaccat
    12601 ttccaaaaca cagcgtcgct gtaccgtggg ttatctgccc aaccgcagcg acacgccggt
    12661 gatcaaaatc agcgccaaat ggctgagaaa agcgggattt gagatcggca cgggcgttac
    12721 cgtgaagatc tcggagggat gtttgatcct gcttgggata acaacgaagt acaggaactg
    12781 cgcagggaac tttatcaggt taaacagtca gttaagggga tgcgagacgg gatgtttagt
    12841 gtgctgaacg aaagttaaat
//
LOCUS       DAJVQH010000115        12817 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJVQH010000115 DAJVQH010000000
VERSION     DAJVQH010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.115"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..490
                     /locus_tag="OQM87_003261"
     CDS             <1..490
                     /locus_tag="OQM87_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1509291.1"
                     /translation="VSVATRQVAGNTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTA
                     RIRVNRGNLPAIKLNTTRRRAGEGLRVGKYFFRGAFVQQLANGRWHVLRCLPEARFAT
                     GHDHQGRLRKNRLPVEVVKIPMSGPLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHL
                     SK"
     gene            487..886
                     /locus_tag="OQM87_003262"
                     /pseudo
     CDS             487..886
                     /locus_tag="OQM87_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            897..1637
                     /locus_tag="OQM87_003263"
     CDS             897..1637
                     /locus_tag="OQM87_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCS1509292.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1696..2082
                     /locus_tag="OQM87_003264"
     CDS             1696..2082
                     /locus_tag="OQM87_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCS1509293.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2091..2420
                     /locus_tag="OQM87_003265"
     CDS             2091..2420
                     /locus_tag="OQM87_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCS1509294.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2392..5331
                     /locus_tag="OQM87_003266"
                     /pseudo
     CDS             2392..5331
                     /locus_tag="OQM87_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5331..5660
                     /locus_tag="OQM87_003267"
     CDS             5331..5660
                     /locus_tag="OQM87_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1509295.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5660..6358
                     /locus_tag="OQM87_003268"
     CDS             5660..6358
                     /locus_tag="OQM87_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS1509296.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYDRFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            6363..7106
                     /locus_tag="OQM87_003269"
     CDS             6363..7106
                     /locus_tag="OQM87_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1509297.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            7043..7644
                     /locus_tag="OQM87_003270"
                     /pseudo
     CDS             7043..7644
                     /locus_tag="OQM87_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            7705..11184
                     /locus_tag="OQM87_003271"
     CDS             7705..11184
                     /locus_tag="OQM87_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1509298.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLERWDTPKVVKGVSFLLRLTVAADDGSERLVSTGRTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDYAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKTLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTTNKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            11252..11851
                     /locus_tag="OQM87_003272"
     CDS             11252..11851
                     /locus_tag="OQM87_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS1509299.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11915..>12817
                     /locus_tag="OQM87_003273"
     CDS             11915..>12817
                     /locus_tag="OQM87_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1509300.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPWYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKTANHLSEIAAAG
                     EKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSD
                     FLAWVRNALPGEYSVAGPYGIIIPD"
BASE COUNT         3020 a         3055 c         4087 g         2655 t
ORIGIN      
        1 ggtctcggtt gccacccggc aggttgccgg gaataccgtt gcgggcgata accaggtgaa
       61 agggatcccc ctgaaactgg tacgtcagcg tgtccgggtg tttaaagcca gtccgtcagg
      121 aaaaatgacg gccaggatcc gcgttaaccg gggcaatctg cccgccatca aactgaacac
      181 aacacggcgg cgtgctggtg aaggactgag agtgggaaaa tactttttcc ggggggcatt
      241 tgttcagcaa ctggcgaatg gccgctggca tgttctgagg tgtcttcctg aagcgcgttt
      301 tgcaacaggg catgaccatc agggcaggct aagaaaaaat cgtcttcctg tggaggtagt
      361 gaaaatcccg atgtccggac cgctgacaca ggcatttgaa gatgcccgcg atcgcatcat
      421 tgctgcggaa atgccgaaac agctggggta tgccctgaaa caacaactga ggttacatct
      481 gagtaaatga accggcacac acaaatccgt cagagcgtgc tggcacgcct tcgggaacag
      541 tgtggagaca gcgccacgtt ttttgacggg cttccggcat ttattgatgc gcaaactgcc
      601 tgccgtggcg gtgtggctga gtgatgctca gtacaccgga aaaatgacgg atggagatga
      661 ctggcaggct gttctgtata ttgccgtctt catccgggca caggcaccgg attcagagct
      721 ggatatgtgg atggagagca ccattttccc ggccctgaat gatgtaccgg cactttccgg
      781 actcatcgac accctgaacc cactcggttt taactatcaa cgtgataatg agatggctac
      841 ctgggcgatg gcggaaatca cgtaccagat cacgtacacg aattaaggag gtggtaatga
      901 ccacaccaaa tccactggca aaaacgaaag gtgcgggaac gacgttatgg atgtataccg
      961 gcaacggcga cgcatttgcg aaccctttgt cggacactga ctggctgcgt cttgcgatgg
     1021 tgaaggatct gcaacctggc gagatgaccg ctgatgcaga agatgacact tatctcgatg
     1081 atgaagatgc agactggaaa acgacaaccc aggggcagaa atccgtcggt gatacttcgg
     1141 cgacgctggc ctggcgtccg ggtgacagcg ggcagaaaaa actggttcag ttgttcgatt
     1201 ccggtgaagt ctgcgcgttt cgtatcaaat accccaacgg tacggttgat gttttccgtg
     1261 gctggctgag ttcactgggt aaaaccatta cctcaaaaga ggtgatgacc cgtactgtaa
     1321 aaatcagcgg tgtggggcgt ccgtatctgg cagaggaggg cgctgaaacc gtgggtgtta
     1381 ccggactgac ggtggcaccg gcatctgtca gtgtcaaagc gggagcaacc accacactga
     1441 cctttacagt aaaacctgac ggggccagtg acaaagcgat cagtgtgcat tcgtcagatc
     1501 ctcagactgc ttcggtgccc ctgagcgggc ttgtggccac ggtgaaaggc gtgcagcagg
     1561 gcagtgtcag catcgtgggc atgacctctg acggagagtt tgtggcagtg gctgcggtga
     1621 ccgtcagcgc accataacag gacgatactc atcattgccc cggttatccg gggctttttg
     1681 catccggaga acatgatgtt tctgaaacag gatacgttta attatgaaaa acagtccgtg
     1741 gtgctcagtg agctgtccgg gctgcagaga attgaatatc tgacgtttgt tcagcagcga
     1801 aaggcaaagt ttgatgcaca ggagggagaa ctgccggagg ctgaacgaca gattgctttt
     1861 ctgcgtatgg gaatggatat caatgcctgg ctggtttccc gctcactgtg gaatgctgag
     1921 cagtctcagg atgttgagac gctttgcgca tccattatga caacatggtc gtatgatgcg
     1981 ctgggcgcgg gggcggagag ggttctgtcg ctgagcggta tggggaccat tgagaatgcc
     2041 ggggatgatg atcatgaggc gctgacgccg gaaaagtcct gacgcgggaa atgcagtttg
     2101 tcatgcggct tgcccgggag ttccggcggg cagactggcg gcagatgctg tcggatatgt
     2161 cggccactga gcttggtgag tggggcgatt atttccggat gcagagcttc agtgatgtgt
     2221 ggatggatgc gcagtttgcc tcgctgaagg cattgatcgt gagaatggtg tccggcagca
     2281 gcgatgctgc ggtggctgat ttcagccttt taccggaaga gaacgggata ccggagcgaa
     2341 cggacgaaga actgatgcat cttggggaag gtatttccgg aggtgtgcgt tatggaccag
     2401 atagccaacc tggtcattga tttggggatt gatgcggcag agtttaaaaa tgaaattccc
     2461 cgtatcaaaa accttctgaa tggtgcagcc agcgatgcag aacggtcttc tgcccgtatg
     2521 cagcgtttta tggagcgtca gactcaggcg gcccggcaga caatgcaggc ggcttcttcg
     2581 gctgcaacag cagcatcagc ccatgcgcag acggtggaga agaatgcacg ggctcatgaa
     2641 cgcatggccc gtgaggtgga acaaacccgt ctgcgcgtgg atgccctgaa tcagaaaatg
     2701 cgcgaggaac aggcgcaggc cagggcactg gcggaggcgc aggataaagc ggctgccgcc
     2761 ttttatcgcc agattgacag tgtgaaacag gccggtgcgg ggcttcagga attacagcgt
     2821 attcagcagc agatccgaca ggccagaaac agtggcgggg ttggtcagca ggattatctg
     2881 gcgctgattt cggagatcac ggcgaaaacc cgtgccctga cgcaggcaga ggaacaggcc
     2941 acccggcaga aagcggcgtt tatccgccag cttaaagagc aggcaacccg ccagaatctg
     3001 tcgtcttctg agttgcttcg cgccagggcg gctcagctgg gggtgttaat cagtcagatg
     3061 gcgcgcggca attttggtgc gctgagggga tccgggataa cgctggctaa ccgtgccgga
     3121 tggacaggcg cactgatgtc gccgaaaggc atgatgactg gcggcgttat tggcggactt
     3181 gtcgcggcgg tcctgggtct gggtaaagcc tggcatgacg gcggaaggag ggcgaggaat
     3241 ttaaccgtca gctggcgctg acgggacatt atgccggtgt cactgtcggg cagttgtgga
     3301 aactcagccg ggccatatcc gggaatggta tcacgcaaca tgcggcagcc ggtgcgctgg
     3361 cacaggtggt gggcagtggg gcatttcatg gaaacgatat tggtatggtg gcgaaagctg
     3421 ccgcacagat ggagcgatcg gttggccagt cggtcagcga taccataaat cagtttaagc
     3481 ggctgaagga tgatcctgta aatgccgcga aggcgctgga caatgcgctg cattttttga
     3541 ctgccactca gcttgagcag atacgcgtcc ttggggagca ggggcggtcc agtgatgcgg
     3601 cccggatcgc catgtctgcg ctggcagagg aaaccggtaa acgcacatct gatattgata
     3661 ataatctcaa tgcgctgggt agtacgctgc aaaccttgtc tgactggtgg aagcagttct
     3721 gggatgcggc catgaatatt ggtcgtgaag actcgctgga tgcgcagatt gatgcgttac
     3781 aggaaaaaat tcagcgcgcg aaaaaatatc cgtggacaaa cgcctccaca caggtggagt
     3841 acgatcagca gcgtcttaac gatcttcagg agaaaaaacg ccggaaggat ttgcaggatg
     3901 caaaagcgca ggcagaacgg aattaccagg agcaacagaa acgccggaat gctgaaaatg
     3961 ccgcgctgaa ccggatgaat gaaacggaag ctgcacgaca tcagcgggaa attgcgcgta
     4021 ttaatgccat gcagtacgcc gaccaggctg tcagggatgc ggcgatacag cgtgaaaatg
     4081 aacgttacga aaaagccatt aagaaaaata cacgggcaac ccgtaatgat gaggccaccc
     4141 ggttattgct gcagtacagt cagcagcagg cacaggtgga agggcagatt gctgctgcca
     4201 gacagtcagc aggcattgcc actgaaagga tgactgaagc gcataaacag cttctggccc
     4261 tgcagcagcg catcagcgac ctggacggga aaaagctgac ggcagatgaa aagagtgtgc
     4321 tggcccgtaa gaatgagctg attcaggcgc tgacgctgct ggatgtgaaa cagcaggagc
     4381 tgcagaaaca gacagcgctt aacgacctga gaaaaaaaac ggttcagctg accagccagc
     4441 tggcagacaa agaacgtgca ctgcgtgagc agcacaatct ggatattgcc actgcaggta
     4501 tgggggataa gcagcggcag cgctaccagg cacagttgcg catccggcag gaataccggc
     4561 aacagttgca acagcttgag aatgacagtc gccagaaagg cacttacggg acggaggact
     4621 accggagggc tgaggaggtg ctgaagggga gcctgaagcg acaactgaat gaaaacaaac
     4681 gctactggca ggaactggaa gtggcgcagg gcgactggaa aaacggtgcc atgcgggcgt
     4741 ttcagaattt tacggcggat gcggataatg cggcgggaac ggcagaacag atgtttacag
     4801 tggcattcag cagtgccggt aatgcactgg cgacattctg taccaccggt aagctgaatt
     4861 ttaaatcctt cacctcttcc ctgttgtcag atatggccag aattatggca cagatggcca
     4921 tgatgcaggc ggtaaagggc gtcggttctt tattcggctt cacgactaat gctgatggcg
     4981 gtgtttacca gtctgctgat ttgagtcgct acagtggcac ggtggttaac cgtccgacgt
     5041 tttttgcttt tgcaaaaggc gcgggtgtga tgggggaagc gggacctgaa gccattctgc
     5101 cactgcgtcg cggtgctgat ggtaagctgg ggggttgtgg cggatactgg tggttcaggc
     5161 atggtgatgt ttgccccgca gtacaacatt gagatcaata acgacggcac gaacgggcag
     5221 ataggtccgg ctgccctgaa ggtggtttat gacctcggga aaaaagcggc cgcggacttt
     5281 atgcaacagc aggcccgtga tggtggccgg ttaagtggag cataccggta atggagacgt
     5341 ttcactggaa agtgcgcccg gatatgaatg tggtatcaga gccgaaagtg gtgacagtga
     5401 agctgggcga tggttatgaa cagcgtcgtg cggcgggact gaataaccag ttgtcgactt
     5461 acagcgtgac gatacgtgtt cgtaaaggtg aacacccatc tttaaaagcc tttctggaac
     5521 ggcacggtgg cgtccgcgca tttcagtgga cgccacctta tgactggaaa ctgatcaggg
     5581 aggtttgtcg taaatggtcg gcaagcgtgg gggcgttatg ggtgactgta acggccgatt
     5641 ttgaacaggt ggtgaactga tgcaggatat ccggcaggaa acactgaatg aatgcacccg
     5701 tgcggagcag tcagcccggg tggagctctg ggaaatcgat ctgacagagg tcggtggtga
     5761 gcgttatttt ttctgtaatg agcagaacga aaaaggtgag ccggtcacct ggcaggggcg
     5821 gcagtatcag gcatacccca ttcagggggc ggggtttgaa ctgaacggca ggggctgtgc
     5881 tgcccgtccg acactgacgg tctctaacct gcacggcatg gtcaccggga tggcggaaga
     5941 tctgcagagt ctggtcggcg gaacggtggt ccgccgtaag gtttacgacc gttttctgga
     6001 tgcggtgaac ttcgtcaacg gaaacagcga cgccgatccg gaacaggagg tgatcagccg
     6061 ctggcgcatc gagcagtgca gcgaactgag cgcggtcagt gcctcttttg tgctggccac
     6121 accaacggag acggatggcg cggttttccc ggggcgtatc atgctggcga atacctgtat
     6181 gtggacttac cgttctgatg agtgtggtta cacgggcagg gcagtggctg acgagttcga
     6241 caaaccaacg acggatatcc ggaaggacaa atgcagcaag tgtatgcgcg ggtgtgagtt
     6301 gcgcaacaat accggtaatt tcggcggttt cctttccatc aataaacttt ctcagtaaat
     6361 ccatgacaca gacagaatca gcgattctgg cgcacgcccg gcgatgtgcg ccagcggagt
     6421 cgtgtggatt cgtggtgaga acgccggagg gagacaggta tcttcccagc gagaatatct
     6481 ccggtgagcc ggaggaacgg ttccggatgg ctccggagga ctggctgcgg gcacaaatgc
     6541 agggtgagat tgtggcactg gtccacagtc atcccggtgg tctgccctgg ctgagtgagg
     6601 ctgaccggcg gctgcaggtg cagagtgatt tgccgtggtg gctggtctgc cggggggcga
     6661 ttcacaagtt ccgctgtgtg ccgcatctca ccgggcggcg ctttgagcac ggggtgacgg
     6721 actgttacac gctgttccgg gatgcttatc atctggcggg gattgagatg ccggattttc
     6781 accgcgggga tgactggtgg cgtcacggtc agaatctcta tctggataat ctggaggcca
     6841 cagggctgta tcaggtgccg ttgtcatcag cacaaccggg cgatgtgctg ctgtgctgtt
     6901 ttggttcatc ggtgccgaat catgccgcca tttactgcgg cgatggtgag ctgctgcacc
     6961 atattcctga acaactgagt aaacgagaga ggtataccga caaatggcag cgacgcacac
     7021 actccctctg gcgtcaccgg gaatggcacg catctgcctt tacggggatt tgcaacgatt
     7081 tggccgccgc atcgaccttc gtgtgaaaac gggggctgaa gccatccgcg cactggccac
     7141 acagctcccg gcgtttcgtc agaaactgag tgacggctgg tatcaggtgc gcattgccgg
     7201 gcgtgatgca ggtgaaaccg aattgtctgc ccgtcttaat gagccgctgg caaatggtgc
     7261 agtgatccac atagtaccgc gtctggtggg agctaaaagt ggcggtgtgt ttcaggcggt
     7321 gctgggggca gctgttatgg cggttgctat atggatgccg gggtaggaat tatggcgagt
     7381 aatctgctgt tttctctcgg tgccagtatg acgcttggcg gtgttgcaca gatgctggca
     7441 ccgaaagcca gaactccccg tacacagaca acggataacg gcaaacagaa cacctgtttc
     7501 tcctcactgg ataacatggt tgcccagggc aatgttctgc ctgttctgta cggtgaaatg
     7561 cgcgtggggt cgcgggtggt atctcaggag atcagcacgg cagacgaagg ggatggtggt
     7621 caggttgtgg tgattggtcg ctgatgcaaa atgtttcatg tgaaaccgcc tgcgggcggt
     7681 tttgtcgttt atggagcgtg aggaatgggt aaaggcagca gtaaggggca taccccgcgc
     7741 gaagcgaagg acaacctgaa atccacgcag ttgctgagtg tgattgatgc catcagcgaa
     7801 gggccggttg acggtccggt ggatggatta aaaagcgtgc tgctgaacgg tacgccggtc
     7861 ctggacagcg aggggaagac aaacttttcc ggtgttacgg tggtgttccg cgccggcgag
     7921 caggagcaga caccgccgga ggggtttgaa tcttccggct cagagactgt gctgggtacg
     7981 gaagtgaaat acgacacgcc gatcacccgg accatcacgt cggcaaacat tgatcgtctg
     8041 cgcctgacct tcggtgtgca ggcactggtg gaaaccacct caaaggggga ccggaatccg
     8101 tcggaagtcc gcctgctggt tcagatacag cgtaacggtg gctgggtgac ggaaaaagac
     8161 atcaccatta agggcaaaac cacttcgcag tatctggcct cggtggtggt gggtaacctg
     8221 ccgccgcgcc cgtttaatat ccggatgcgc aggatgacac cggacagcac cacagaccag
     8281 ctgcagaaca aaacgctctg gtcgtcgtac accgaaatca tcgatgtgaa acagtgctac
     8341 ccgaacacgg cgctggtcgg cgtgcaggtg gattcagagc agttcggtaa ccagcaggtg
     8401 agtcgcaatt atcatcttcg cgggcgcatt ctgcaggtgc cgtcgaacta taacccgcag
     8461 acgcggcaat acagcggtat ctgggacgga acgcttaagc cagcatacag caacaacatg
     8521 gcctggtgtc tgtgggacat gctcactcat ccgcgctacg gcatggggaa acgtcttggt
     8581 gcggcagatg tggacaaatg ggcgctgtat gtcatcggcc agtactgcga ccagtcagtg
     8641 ccggacggat ttggcggcac ggagccgcgc atcacctgta acgcttacct gaccacacag
     8701 cgtaaggcgt gggatgttct cagtgatttc tgctcggcga tgcgctgtat gccggtatgg
     8761 aacgggcaga cgctgacgtt cgtgcaggac cgaccgtcgg ataaagtgtg gacctataac
     8821 cgcagtaatg tggtgatgcc ggatgatggc gcgccgttcc gctacagctt cagcgccctg
     8881 aaggaccgcc ataatgtcgt tgaggtgaac tggattgatc cggacaacgg tcatgagacg
     8941 gcgacagagc ttgtggagga cacgcaggcc attgtccgtt acggtcgtaa cgtcacgaag
     9001 atggatgcct ttggctgtac cagccgggga caggcgcacc gcgccgggct gtggctgatt
     9061 aaaacggaac tgctggaaac gcagaccgtg gacttcagcg tcggcgcaga agggcttcgc
     9121 catgtaccgg gtgatgtcat tgaaatctgc gatgatgatt atgccggtat cagcaccggt
     9181 ggtcgcgtac tggcggtgaa cagccagacc cggacgctga cgctcgaccg tgaaatcacg
     9241 ctgccgtcct ccggtaccac gctgataagc ctggttgacg gaaatggcaa tccggtcagc
     9301 gtggaggttc agtccgtcac cgacggcgtg aaggtgaaag tgagccgggt tcctgacggc
     9361 gttgcagaat acagcgtgtg ggggctgaag ctgccgacgc tgcgccagcg cctgttccgc
     9421 tgcgtgagta tccgtgagaa cgacgacggc acgtatgcca tcaccgccgt gcagcatgtg
     9481 ccggaaaaag aggccatcgt ggataacggg gcacactttg acggtgaccg gcgcggcacg
     9541 gtgaatggtg tcacgccgcc agcggtgcag catctgaccg ccgaagtcac cgcagacagc
     9601 ggggaatatc aggtgctgga gcgatgggac acgccgaagg tggtgaaggg tgtgagcttc
     9661 ctgcttcgcc tgaccgtggc agcggacgac ggcagtgagc ggctggtcag cacaggcagg
     9721 acgacggaaa ccacataccg cttcaggcag ctggcgctgg ggaattacag tctgacagtc
     9781 cgggcggtaa atgcccgggg gcagcagggc gatccggcgt cggtatcgtt ccggattgcg
     9841 gcaccggcag cgcctgtcac tattgaactg ataccggggt attttcagat aacggtggtc
     9901 ccgaaactgg ctgtatatga cccgacggtg cagtttgagt tctggttttc ggaaaagcgg
     9961 attgcggata tcaggcaggt tgaaaccagc gcccgctatc ttggcacggc gctgtactgg
    10021 atagccgcca gtatcaatat caggccgggc catgattatt atttttacgt tcgcagtgtg
    10081 aacaccgttg gcaaatcggc atttgtggag gccgtcggtc gggcgagcga ttatgcggaa
    10141 ggttatctga gtttttataa agggttgatc aataaaacgc atctcggcaa ggagttgtgg
    10201 acgcagattg ataacggtca gcttgcgccg gacctgactg aaatcaggac gtccattacg
    10261 aatgtcagca atgaaatcac gcaaaccgtc aataaaacac tggaaaatca gagtgctgca
    10321 atccagcaga tacagaaagt tcaggttgat acaaataata acctgaacag catgtgggct
    10381 gtgaagctgc aacagatgaa ggacggacgc ctttatattg cgggtatcgg tgccggtatt
    10441 gagaatacgc cagcaggaat gcagagtcag gtgctgctgg cggcagacag gattgcgatg
    10501 attaatcctg cgaatggcaa cacaaagccg atgtttgttg gtcagggcga tcagatattc
    10561 atgaacgacg tgttcctgaa acgcctgacg gctccgacca ttaccagcgg cggtaatcct
    10621 ccggcatttt ccctgacacc tggcggacgg ctgacggcga aaaatgccga tatcagcggt
    10681 aacgtgaacg cgaactccgg gacgctcaac aacgtcacga ctaataagaa ctgtcgggtt
    10741 ctgggaaaat tgtccgcgaa ccagattgaa ggcgatctcg ttaaaacagt gggcaaacct
    10801 ttcccacggg actcccgggc accggagagg tggccatcag ggaccattac cgtcagggtt
    10861 tatgacgatc agccgtttga tcggcaaatt gttattcccg cggtggcgtt tcgcggtgct
    10921 aaacatgagc ggaagaataa caatatttat tcgtcatgcc gcctgatagt gaagaaaaac
    10981 ggtgctgaaa tttataaccg aacgaccctg gataatacgc tgatatatac gggtgttatt
    11041 gatatgcctg ccggtcacgg tcacatgacg ctggagtttt ctgtatcggc atggctggta
    11101 aatggctggt atcccacagc aagtatcagc gatttgctgg ttgttgtgat gaagaaagcc
    11161 actgcaggca tcacgattag ctgaatttta taacccagat acgggcacca gaaatggtgc
    11221 cttttttatt gcagaaaagc gagaggtaat tatgcgtaaa gtttgtgcag ccattttgtc
    11281 cgcagccatc tgtctggctg tatccggtgc gcctgcatgg gcgtctgaac atcagtccac
    11341 gctgagcgcc gggtatcttc agccccatac tgatatgcca ggcagcgatg acctgaaggg
    11401 cattaacgtg aaataccgtt atgagtttac ggacacgctg gggctggtga cgtcattcag
    11461 ctatgccaac gctgaagatg agcaaaaaac gcattacagc gatacccgct ggcatgagga
    11521 ttccgtgcgt aaccgctggt tcagcgtgat ggcggggccg tctgtgcgcg tgaatgaatg
    11581 gttcagcgcg tatgcgatgg cgggcgtggc ttacagccgt gtgtcgacct tctccgggga
    11641 ttatctccgc gtaactgaca acaaggggaa aacgcacgat gtgctgaccg ggagtgatga
    11701 caaccgccac agcaacacgt ctctggcgtg gggggctggt gtgcagttta acccgaccga
    11761 atccgtggcc attgaccttg cttatgaagg ttccggcagt ggcgactggc gcactgacgg
    11821 tttcattgtg ggtgtcggtt ataagttctg attagccagg taacacagtg ttatgacagc
    11881 ccgctggttc aggcgggctt tttgtggggt gaatatggca gtaaagattt caggtgtact
    11941 gaaagatggg gcgggtaaac ctgtcgtaaa ttgtgcgatt gaactgcggg ccagaagaac
    12001 cagtccgacc gttgtggcac acgttgttgc cacttgcgtg acggacaata acggtgctta
    12061 tgtgattgag gctgagccgt ggtattacga ggttgcgctt cactgtaacg gctggcagcc
    12121 aacccgtgtc ggggatattg atgtggcacc gactgatgca ccggggacac tgaacgcgtt
    12181 tctgaatgca ccaaaggatg gtgatttacg tccggaggtg atgaagcgct ttgaggaaat
    12241 ggtggcgcag gcgcagcaga gtgccggggc tgcagccgga aacgcacagc agacggcgca
    12301 ggatgtggcg gcagccgcaa cggcccgtga tgatgcacaa cgttttgcgg agaaagcccg
    12361 acaggatgca accgtcacag ctgaggacag aaaggccact gcggaagatg tgacaagcac
    12421 aggagcaaat gcagccgcag ccggacagag cgcacaggat gccgcaggtt atgcccgcgc
    12481 agcagaacag gccaaaaatg acattgatgc tgcgctgacc ggcaccctga aaacggctaa
    12541 ccatctgtct gaaatcgcag cagcaggcga aaaggcacaa cagaagtccc gggataatct
    12601 ggggctgaaa agtgcggcca cgatggaagc acagagcgac atttacgacc ggacaaaagg
    12661 ccgtctggcg atacccggcg cattcggctt tgggtgtgct tttctgcctg aagatgttat
    12721 ccgttttgac actaagagtg atttcctggc ctgggtaagg aatgcgctgc caggtgaata
    12781 ttccgttgct ggcccttacg gcatcatcat acccgac
//
LOCUS       DAJVQH010000116        12807 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJVQH010000116 DAJVQH010000000
VERSION     DAJVQH010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.116"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(68..2017)
                     /locus_tag="OQM87_003274"
     CDS             complement(68..2017)
                     /locus_tag="OQM87_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1509301.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2102..2438)
                     /locus_tag="OQM87_003275"
                     /pseudo
     CDS             complement(2102..2438)
                     /locus_tag="OQM87_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            complement(2438..2794)
                     /locus_tag="OQM87_003276"
     CDS             complement(2438..2794)
                     /locus_tag="OQM87_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCS1509302.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2794..4290)
                     /locus_tag="OQM87_003277"
     CDS             complement(2794..4290)
                     /locus_tag="OQM87_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCS1509303.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAH
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4287..4448)
                     /locus_tag="OQM87_003278"
     CDS             complement(4287..4448)
                     /locus_tag="OQM87_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCS1509304.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4458..5021)
                     /locus_tag="OQM87_003279"
     CDS             complement(4458..5021)
                     /locus_tag="OQM87_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509305.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLSNERDEKGQWAAYDAVHDVRRELWKALLG
                     WMPDPQGGEIVYVGGTLLDLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLS
                     IDVDYIDPGTGPDGDIEHHLEMRFPQN"
     gene            complement(5018..5542)
                     /locus_tag="OQM87_003280"
     CDS             complement(5018..5542)
                     /locus_tag="OQM87_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509306.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            complement(5508..5924)
                     /locus_tag="OQM87_003281"
     CDS             complement(5508..5924)
                     /locus_tag="OQM87_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCS1509307.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            complement(5921..6244)
                     /locus_tag="OQM87_003282"
     CDS             complement(5921..6244)
                     /locus_tag="OQM87_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCS1509308.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            complement(6289..7392)
                     /locus_tag="OQM87_003283"
                     /pseudo
     CDS             complement(6289..7392)
                     /locus_tag="OQM87_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(7393..8019)
                     /locus_tag="OQM87_003284"
                     /pseudo
     CDS             complement(7393..8019)
                     /locus_tag="OQM87_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
     gene            complement(7997..9238)
                     /locus_tag="OQM87_003285"
     CDS             complement(7997..9238)
                     /locus_tag="OQM87_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1509309.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(9238..9420)
                     /locus_tag="OQM87_003286"
     CDS             complement(9238..9420)
                     /locus_tag="OQM87_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509310.1"
                     /translation="MFLIILTPLVGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(9432..11189)
                     /locus_tag="OQM87_003287"
     CDS             complement(9432..11189)
                     /locus_tag="OQM87_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1509311.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSAGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(11189..11671)
                     /locus_tag="OQM87_003288"
     CDS             complement(11189..11671)
                     /locus_tag="OQM87_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCS1509312.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(11819..12169)
                     /locus_tag="OQM87_003289"
     CDS             complement(11819..12169)
                     /locus_tag="OQM87_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1509313.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(12212..12676)
                     /locus_tag="OQM87_003290"
     CDS             complement(12212..12676)
                     /locus_tag="OQM87_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS1509314.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(12673..12807)
                     /locus_tag="OQM87_003291"
                     /pseudo
     CDS             complement(12673..12807)
                     /locus_tag="OQM87_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
BASE COUNT         2883 a         3797 c         2968 g         3159 t
ORIGIN      
        1 aaaacaaact ccattgttag catgttacgc gataattttg cctcatcttt caccacaaaa
       61 aaagaactca ccttttaaat ggagaatacc caacatcatg ggtgattttc ataaagggat
      121 cgcctgtttt cggcaaatcg ataacgcgca ttccaggcgg cgcgttgtca aacgtcacct
      181 taagttcgct gcgggttgcc ggtgaagatg caaggcttaa cagctgattc ctttccagtg
      241 atacattcgg ggtgtagcgt ttatcgggag ataaataccc atcaccacgt ggcatctgcc
      301 atccggtcat gtcatagaca aaatcatgga actgtttgcc ccactcgtcg aacttatcat
      361 tcagaccaaa tccactgttc agtgcatcag ccacaaaatt tttaacaagc cagggatgtt
      421 ctttttcaaa ctcctgcgcc atcatgccca gttcaagcaa tccaccaatc agactgatct
      481 ttccgaggcc ctttaatttc agccctgttt ttcctgccgc cgttgtccat tgattctggg
      541 cgactgttgc cgcacgaata cttgccaccg ccttcacacc aatatacagg gcagaaaccg
      601 ttgctattgt ttttactgcc tgttcccatc cccccattgc tctgacaacc tgatcaacct
      661 cctgccagac ttttttcaca accggaccga ccgtttccca gttatcaatt atgagatatg
      721 caccaccgac gagaagcgca attaatccct ttgccggcgt catattcatc acgctaccca
      781 tgatttttgt tatgcgtgtc aatgtgccaa tcgccacgcc catcgtcagt aatgccgcac
      841 cagttttcgc gatggttttt acgacctcag gattttctct gacaaacgtg cgcacttctt
      901 ccagaaaagg cttcatctct tttatgcctt cattgagtga aggcaggaag gtttccccca
      961 gcgtggaaga aatcgcattg atctggttct gcagcagcaa caactgattt tctgtcgtcg
     1021 cggcacgggc ggcatattct ttctgcattg agccgccata ctgctgcgca tccgccacac
     1081 gattaaagtt ggttcgcaac aaatccagat tcgtgagcag cggcgcaatg gcccccaggg
     1141 attccttccc gaacagcgct ttcagcacag cagcctgttt ttctttcggc acttttgcca
     1201 gagaatccag tacgtgcagc atggccccgc gggcgtcttt ctgcatatcc gccgccagtt
     1261 ttttcgggct aatgcgcaac gagcggagca cttttttctg cgatttggtg gcagaatccc
     1321 tcgctgtcag cgacaacata aaattcttta tccccgtcgc agcaatttct gactccaccc
     1381 ccattccggc aatggttgcc cccattgcgg caatctctcc ggaggccaca cccgcaacac
     1441 tgcctaaagg gccaatacgg gtcacaacat cagaaatctt tttcgcactt gcaggaccgg
     1501 tattgccaag atagttaatc ttatccgcaa gccctgccac ctctccctgt gtcagtttaa
     1561 aggcagtgcg ccactgcgcc atcatctgac cggattcttc tgccgtggcg tcaaaggcca
     1621 cgcccatctt cacggcatcg tcagtaaact gcatcagctc gtcacgggcg ataccagcct
     1681 gaccacccgc cgccacaatt tcggcgatac cttccgccga catgggcagt tctgttgaca
     1741 ggtcgcgcac ctgctccgtc attgccttaa acgcttccgg cgtatccaga ccatccacca
     1801 ctttccggac atccgccatt tttgattcaa gggcaatggc agatttgacc gggagcgcca
     1861 gcgcccccag tactgcggtt ccggcacccg ctgcgcccag tgacaggctg gcaaactcct
     1921 ttttaaaacc tttcagctgg cgctgcattc ccttcagcgg tgccgatacc ttatccacgg
     1981 cagtgatgat cgccttcagc tgaaaactgt cagccatgct tcatctcctc gttaatgcgg
     2041 acggcctcgg cttccagttc tgtaaactgc gaaacagcca cccgtcgcag ctccagtgga
     2101 ttcagtttcc agaaccaggc aacattgtag agtcgtttcc cggaggtgct tcccgtctcc
     2161 gactgggtaa aaaaacgcag gatctgcatg ctggtcttaa aaatatccag ttttgccatc
     2221 tgcgccgccg atgaacgcgg gatccccgcc agcaacggga tatatttcag tgccacctgg
     2281 ctgtccagtt taatactgcc ttcaccggaa ataatgaaag gaaaacccag cgcctcgatt
     2341 tcgtcatacg tgggctcgcg caactccagc acatgcagca tttcgttatg cgctgtcacc
     2401 ggttttttta aaactatttc tgtaacgttc attgccatta ctgataccct ccctcttcgc
     2461 cgtggaattc aagatctgcc gtgccttctt cggcattatg attagcctct ccgtgcagcc
     2521 atgccgccga aagcacatac accatgccgt ttgccagctc agcggtaatc gtcatctggt
     2581 ctgaggtggt aattttgtcg accggaaagt ttttgggcac tttaaacgtg cccttaacgt
     2641 aaggtgaccg ccaggtctcc ttgtaatcca catcccctgc catgccgacg atgtcatccc
     2701 ggacattggt gttcatcggc acttcaatgc cacctgtcag cgataactgc tgaccatcca
     2761 ctttaaaaaa acaggttccg gcgatctttg ccattatgct gactcctctg aatactgaag
     2821 acggaactgg ttaaccaccg caaagacacg taactgatta acataatccg gcgggaacag
     2881 cgtattcagt cggttcgggt tatctgcatc acgctcaact atcagatact gtttaaacag
     2941 atcgtaattt tccacaatac cggcacgctc catctgacga tatgtcgcca gaagttcccc
     3001 tttgataacg gcaggagtga caatcgcctg ccccggacca aaacgggtac catcatttgc
     3061 cagcttgtga cgtccgtact tgcttgtgat gaccgatttc agtttgcgaa gaacatgtgc
     3121 actggtatgc agagtttcac tgtccagata gctgttgtct gccacgccat acgcattctt
     3181 tttgtaggtg gttacggaac gctggatctg caacgtgccg ccttccacat aagccgtcgc
     3241 cacgccgtga gataaaaggg tctgctgctc tgtcatgatg aatcgcttac ctttcggtgc
     3301 cggaagcatc cccaccagct cccccgtctg tgtcggacgg gcaggatcat tccggataaa
     3361 taccgcttca cgggcaaggc gactggcaac cagttcatcg acaggcgact gggtttcttt
     3421 ttcgtaaccg gcaagcgtga tatgttgctg attatgcata tctccggcac caaccagctc
     3481 tgacagcgtt cccagttttg cggtatagac atgcccgtat aactggcgcg cataactcca
     3541 gcgaccgctg ctgtcattca tttcggtcat catcatattg atggaggcgg catcgttgaa
     3601 cggcagaccg ataaagtcga atgcctcatc gcccatagca gcaacagcgg cggtaagatc
     3661 aggcgctccg ctaccggcag caccgacttc cgtcaccacc cgaagccctg ctggcagaat
     3721 ctcaccacca ccagaaccat aataattcag acagaccggt aactcattac catacagccc
     3781 cttatgacgg gcagtcagcg tcaccacgcc tgcatcagat gacgccgtaa acggcagggt
     3841 gataaccccg tttaccgctt ccttaatcgc ggtggcaacc gcagtggcat catcgccatt
     3901 caccacaggc acctgtacac gggagcgccc gacatacaga ctcagggtgc cgctttcctc
     3961 tgcttctccg gcaaccgtca ccctgaccgt cgccgccgcc cctctggctt ccggtactgc
     4021 aataacatac agttcaccga aaggatctgt ctgacggtag acttccacca tacgcgccag
     4081 ctggctcccc gccccacaaa tctggcgggc ataatctgcc gatggcatca gcaccaggct
     4141 gttaacctca atggctgcat cgttgctggc atgcccgatc agtaatgcag gcgcgctggt
     4201 caccgctgta tttgccgcag aattgttcat ctcggcgtag aacaacggca ccagcgtatt
     4261 cgacggaaca gtattaaaac ttatcgtcat gattttttag ccttatcctg tttaacacgt
     4321 accacatcac ccgccgctat acggcggaac cagtagctgc tttcttccac atttcgccct
     4381 tcggaaggca aaaggtctcc acgggcaggg tcaggaaccg accgcccttt caggggtttc
     4441 acaaacatga gggactctta attctgaggg aaacgcattt ccagatggtg ctcaatgtca
     4501 ccatccgggc cagtaccagg atcgatgtaa tccacatcaa tactcagcag ggataaatcc
     4561 ggcagggcat tcacgtcctc tgcctgtcgc gtgtcttctt ccgtgatttc atacttcgcc
     4621 gtaaaatcaa actggtaata cagttcgtaa cggttcagat ccagcagggt gccaccgaca
     4681 taaacaatct cgcccccttg cgggtccggc atccatccaa gcagggcttt ccagagttct
     4741 ctccggacat catgaacggc gtcataggct gcccactgcc ctttttcatc acgctcattg
     4801 ctgagcacca caatcacgga gaagccttca gtcaaatcct gccagtaatc ggtctgtgat
     4861 ttttgttccc ccggcgcatc gtcagaggga accacatagg cagcaggcag gcgaagtttt
     4921 ccggcatcag ggatcgcctt aaactgcgcc gcgccaccaa cacgctcctc aaaacgcggg
     4981 catcgctcac gcagcgccgc aattatcgtt gtcagtttca tttatgcttc ctttttaccg
     5041 gacgtaacga acgctgcagc tcactggaca acagttcctg cgtccagtga cgctgacgct
     5101 caataacgtc agccataaag ttattacgcg gggccagccg gaaagtcgaa gaatggtgct
     5161 tcttctgccg cttatccttt ttatccattc catacgctga atggcgaacg ccgtaataca
     5221 gaaacgccgg ataataagga gcaccttcag gaaaacggcg attcccctgc ccgtttttct
     5281 ggtcagggga aattttcacc atcagtccgg ggcgacgcgt cgtttttttg ggaacgtaat
     5341 aaccaatgga gcgggccaga cgcccggtct gatatcccgg gttctctccc ggaccggagc
     5401 gtccgcgttt aatcaccaga cgtctggcat cacgcatgta aacgcgcccg atttgcacaa
     5461 acgcccgacg cagacgggcg cgattaaact ccagctcctc tggttgttta aagtcgacgt
     5521 gtaaaaatgc tgtctgattc atggcattca ccccgtcgtc gcgctgtacg cagttcttca
     5581 cattccagta ataaaaaacg ccgctgaccg ttcaggtcac gtattcgccg gatccggtac
     5641 tcttggccgt aataaaccac ttcgtgatct gccgtgatgt cgtgccggaa acggatcgta
     5701 aaataatgcg taacgatatt ttctgtctgc actgagccct gataagcggc agcgcctggc
     5761 tgagccacct ttgcccagac atcaaacgac tccggatacg ttggctccgt accaaaatca
     5821 gcggtgggtt catccacacg aaggcggatc tttatccggc gattcagctc tccgggatcc
     5881 ggtaaaaggt aagtggcact ggtctgactt tgcctgattt tcatagcggg acaatcctgt
     5941 aagggccagc aagccatctg aaactcattg gcgtttccag tttctccaca tcggtgatcg
     6001 ttgagcgatt ttcataaaaa tgactgacca gcatcagcat tgccagacga acgtcatcag
     6061 tcagatgcat cccgtcagga tcatcttccg gaatcgtctc ttccggtgca tacaacttcc
     6121 tgttcaggta tgtctctgtt tttttctgta ccgcctgtgc cagcagtttc agaaaatcgc
     6181 cgtcactgta caaaccatca tcgagccgga gatgagattt aatctcctct tctttcagga
     6241 gcatattttc ctcctgtgcc cgcgattacg cgggcacaaa aaaccgcatt acgcagcggc
     6301 tttctggcgg gttgcagccc caattttcat cagcttaatc gcctgagaat ccaccagcat
     6361 accgccggtt cgcttggtgg tataaaaacc cacgaacggt ttgttggtgt acgggtcacg
     6421 caggatacgg gtaccgatgc gatcaacgat ggtatagccg cgtttgaagt tcccaaacgc
     6481 aatggctttt gcatcggcgg caatatccgg catctgctca ttctcaacga tgccataccc
     6541 tgccagagaa gaaggctgac ccagctcaat acccggacgc cacaaataat ttccgtcgtt
     6601 atccttcagc agacgaatgg caaacaggct gctgttgttc atcatgaact tcgcgccgct
     6661 gcggtgcgcc ttacgcaagg tgtaaatcag tttaatgatc gcatcagcgg tcacgccgga
     6721 agccgcgccg gaagcaatgt gctgaagttt gccaaacgca cgggtcttgt catcttcatc
     6781 ggtggactca taagccagaa aaccttttgg ttttttgcta ccgtcgccac tggtaaaggc
     6841 aatttcttcc tgttcggcaa attccagcgc cagctcactg ttgatccagt cttccacatt
     6901 aaagaaagca tcatcgagca ttttctgtgt ggcctgcggg ttgccgtaga tttcccccat
     6961 aaagggctca atcagtccca gttttgaggt ggcggtttcc ggacgcgctt ccgtttcccc
     7021 cacccatccg gacgtcgtgc cgcccagatt caccagtttt ttataatccg agccactcag
     7081 agtgattacg gtggcctcct ggcgcatcac cacttcatct ttcagcaacg tcagaatggt
     7141 gcgatccagt tcttccggaa tggcataacc accatcttca tcattaccca cctgcagtgc
     7201 ctttcgctca agctcacgca ggccatcttc acgccccttg cgcataaaac cgataaacgc
     7261 ttctttatgt tccccggcaa ctttattttg cgtaccacct gccggacgct taacttcagc
     7321 cagctcagcc tcaagatcgc ttttgagatt ttccagttca gtcagttttc cgttgagggt
     7381 ttccacttct ccgttcagtg cagagcccac atcctcagcg tcgcgcaggg acagtgcgcc
     7441 atagcccccg gccatgaatg ctttggcctg ggtacgggag agtccgacat cacgcaggac
     7501 tctttcgatt tttttctgtt cggggatttc cccgcgggcc agtgcgttct tgacgtcgct
     7561 gatccgcgcc tcgtcgttag acgggaacgt caccagactg acttcccaga ggtcgatttc
     7621 tttcagcaga aaggcttctt tgctccggtc gtattcccag tctttcagga cgtacccaat
     7681 agaaaggccg gttaacgaac cggccttcat gtgtgcatgt gcacgttttg cgaggggatc
     7741 atcatcaata agcaaccgtc ccctgacgta aagcccgaca tcgtcttcct tcatttcggt
     7801 gtaaacaccg atgggttcat ccatgcggtg ctgccagagc agcgcaggta acgcttttct
     7861 gtcactccac gcccgcaggg aagcagcaaa tgccccggac atcaccacat catcgtggct
     7921 gtcctttaca ccaaagacgg agccatgccc ttcaaactca ccggagtcac tgacagattt
     7981 cagactcagc ggtacatcaa gacgttgttt cgtctgcatt ggcgttatcc ttctgcttac
     8041 cggctttact gccatcggag ggtttcgtgg tcatgttcat cggtgtgaga tagacatccc
     8101 caccgggacg cggattcata tcttccaggt cgcggcagtc attgggagag taaatccccc
     8161 agttaatccc ggtggcgtag gcttcaaaac gggatttcat atccccgcgc agtaacgccc
     8221 cggcgttaaa tttggcgtaa aacttcccct gtttgctctc cctgaccagc cctgtattga
     8281 tccgttgttc aatacgggtc agatacggca caagggaata gttgataaat ccgagcccca
     8341 gctcttcaat attgttgaaa gtggcgcgat cggtgttctg caccatgtgc agcggcacgc
     8401 ggaaaagacg acagatttct tccaactgaa acttgcgggt ttccaggaac tggctgtcct
     8461 cggcattcag tgccaccggc ttccagtcca gccccatttc cagaatcatc ggacggtgcg
     8521 cattacccag cccaagatga cgctcctcaa aatccctctt catgcgctca taagcatccg
     8581 gcgtgagctt ttgttccgtg cgcaacacac cggatgtcac cgcaccatta ccaaacaacc
     8641 tggcaccgtg ctcctcggtt gccgctgcca gtgaaatggc ctcgcgcgca tatgcaatgg
     8701 gattcagccc gaccagtccg tccagcgtca gggtgcgcac atgccagatt tcatcctggg
     8761 tcaacacatc cacggaacca tccggaaacg tcacctgata aaccggctgc cactggctgt
     8821 tcagcttcgg ttccacacag cccggatcta tcggaagaag ctccacaact tcccccagcg
     8881 ccttcacctt gtaagcgtaa aaattccccc gcagacacag gcagacgata accagctccc
     8941 agaattcctg cggcgtcatg tagccattgg gttttgctga aatcagctta tgcagccgtt
     9001 catccaccgc ccgtgtttta agggtgccgc tgattttgta gagactgcag ggcagcatac
     9061 caacagactc agccagcacc ctgacgcagg aatagaccgc cgtcagccgc atggcccgct
     9121 ggctgctgat ccgctttccg gtataggtgt cgtatgacaa ccccagctct tccgcaagca
     9181 tcccgggcgt tgtgacgggg gtgttatttt tgcgttgaaa aagcccctgg aaaaacatta
     9241 ctcacctccg gaggcgaccc ggtgaccgcg atcgagataa cgcgcaacca gccacgacca
     9301 gcacagacac agcgccccgg caacaacaaa acctgccggg ggataaatca gccatgtgcc
     9361 atacgacagc aaaagcgccc ccagcacgcc caccagtggc gtgagaatta tcagaaacat
     9421 aatgacctcg gttaaagcga gcgaatacca acgctgacca gatgctcaga cagatccggc
     9481 tccggttcac cgccattgac cagcatccgg ctcattgctg taaacatcgc aacagggccg
     9541 tcgattttgg cttccggcgt ggatttgttc gggaagatat tgtcgttttt gtccggtttc
     9601 accgtaacgt tagacatcat ccagttcatg accgggtgat tactgtgatg gaaacgcccg
     9661 gcatagacca gtgattccgt ttccttcatg gcctctgaca gattgcggac cgtctgcgga
     9721 acttccacca gcggtatacc ttcttcagcc agcgccagac tgaactgcat tgcgctccac
     9781 gggtcaaatc ccagttccct caggttttca ccgccaatcc attccagtaa gtcacttttt
     9841 atctgagcat gatcgataac atcaccatcc gtcagaatca gcttatccat ctccgcccac
     9901 ttccggtaaa gttctgcctg ctgccgcgag catcgttcca gccgtccttc cggaagccag
     9961 aatttaaaat cggcatgaac atgcccgtta tccgttcgcc agagttttgc cgccgcacag
    10021 atatcaatct tatgagcaag gtcaacgccg acccacatgg gatacgtttt cagctcatgt
    10081 cgtggggcaa tgtattcgca cttctcccac ttaatcatgt ccatccaggc agactctgct
    10141 gttacccaca cattcatgtg tttggtaaaa aaattcaccc gcgcagagac ctgttctttc
    10201 gcttttttcg ccaggcgacg cagatcatcc cagcgtttac agatgcccag gccgggattc
    10261 gctttctgcc agaccgtttc atcaaacgga tcatctccct catcgagggt gtaaataatc
    10321 gcaaagtagg agtcgtcttt taccgcgccc tccacgtcgc tgttatagcc acgcaatacc
    10381 ttgatggcgt aatcacgctg ctcgtaacaa attccttcct tgttaaaccc tgccgtggtg
    10441 ataccaaata aaagggactg cagacgggca ccggttgccg tttccagaac gtcccacacg
    10501 tcacgggttt tatgtgcatg cagctcatca ataatggcgc agtggatgtt cagaccatcc
    10561 aggttgtttg catctgaaga aagcggttca aattttgatg cgctctgctc ctgataaatc
    10621 gccagcttgt tgaaatcaaa caaccgcccg agtgtcgacc gggcttttct gaccatattt
    10681 ttggcgtctt caaacacgat tctggcctgg tcacgcgtgg ttgcggctga atacacctca
    10741 gccccgcctt caccatctgc ccccgtcata tacagaccga tacctgctga cagggttgat
    10801 tttgcgtttt tacgggcgac ttcgttgtac gctgtccgga aacggcgcac catcaccggg
    10861 cgtccgctgc catcgctgcg catgacaact tccccggtct cttcattcac cagcggaatg
    10921 acaaaaccaa aaatattaat gaggataaac acatgccagt ccatcaactc aatgggctga
    10981 cctgccagcg ccccttttac atggggcaca aatttgtaga aattcaggat gtgctgcgca
    11041 cggggttcac tgaaataaat cccccgctct tcgccgtact tcagatcatc aagaaaacgc
    11101 tggcaggcca gacggacaaa ttcaccagcg acaatttctc ctgcaacaac acgttcggcg
    11161 tagcggatcc cgtcagccac ttttgccatc agtctctcgc ttttaaaagt tctgccagcg
    11221 gatcaacatc atccggtccg gcgatattta ctttcgcccg gcttgccggt gacataccaa
    11281 attctgcaag catcgcccgg atccgcttcc aggcatccgc tttcatcgca gcagccgggt
    11341 gtgccttaat cagcacatca ccgttctgcg tttccgtgcg gtaggtataa ccctcaacat
    11401 cgagggtttc gcagtgatgc cggtattcgg tgtaggcttc caccagcaac tcgagtgcac
    11461 gcgcatcaag ctgagaaatg atcccttccg cattcagctc ttccgccatt cgcctgaacc
    11521 agtactttcc ctgcgcccct aaatgctgcg gaattttagg gagacctttt tcgtcctttt
    11581 tagcggtttt tttggcgtct ttaacgggac gctttgaggg attgcctcgt atcaaatgca
    11641 ggcgtggcgg ggttttcggg ggtcctgaca taatcgattt tacctatcaa tcgcttgatc
    11701 gcattcccaa aaaaaagttt tcgaacctgc ggcgatgcga ggaagggttg gcgggcggtc
    11761 ccggacagcc agggctgcag ggatttgacc cgcccctccc tacaagtgag aataattatc
    11821 acctgattcg ttcgcgcgct gttttcgctt tatggcaggg ccagcacaga ctctgcaggt
    11881 tgctgtctgc gtctgttccg ccatgcgctt tcgggatgat gtggtcgacg gttttcgcct
    11941 cgctcaccac accgacacgc agacacaact gacacagacc tttatcgcgc ttcagaatac
    12001 gggcacgaat caccgtccat tttgagccat agccacgctg gtggcggctc agtccgcgct
    12061 ggtgctgcac ccagccttca ccgcgatgct tatcgcagta acccgaactg tctgtggttg
    12121 tacctgcaca tccacgctta cggcaggcac gtgggattag tgatggcata aatacctcat
    12181 accctgcgaa atgtttacca cgataaaaag gctacttaat gcactgagtg cggatatact
    12241 cctgtgcccc ttccagttgc atctgcatcg tcatcagccg ctctctgagg gtgaaataat
    12301 cccgttcagc ggtgtctgcc agtcgggggc tggttgcatt atccacgccg gaggcggtgg
    12361 tggcttcacg cactgactga cagactgctt tgatgtgcaa ccgacgacga ccagcggcaa
    12421 catcagcgcg cagagtttca ttttcagctt tcgcatcagc taactccttc gtgtatttag
    12481 catcgagtgc attaacatca cgctgtctct gctgcatgtc agtaatggtt gcgttcgcca
    12541 gcttcagttc tcttgcgttt ttgtcgcgct gctctttgta ggtaatagcg ttatcacggt
    12601 aatgattaac agcccatgac aggcagacga tgatgcagat aaccagagcg gagataatcg
    12661 cggtgactct gctcattgct gcccccacaa acagacttca cgctcaatct cacgacgggt
    12721 catcagccct ttccattgct taccgccagc atatgtccgg cgacgtagct ggtcacatgc
    12781 gcctttgata tcgccctagt ttatttt
//
LOCUS       DAJVQH010000117        12177 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJVQH010000117 DAJVQH010000000
VERSION     DAJVQH010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.117"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(124..291)
                     /locus_tag="OQM87_003292"
     CDS             complement(124..291)
                     /locus_tag="OQM87_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509315.1"
                     /translation="MHFRVTGEWNGEPFDRVIEAEDINDCYNHWMIWAQIAHADVTNI
                     RIEELKEHHAA"
     gene            complement(479..1318)
                     /locus_tag="OQM87_003293"
     CDS             complement(479..1318)
                     /locus_tag="OQM87_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCS1509316.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPVAYIFKHPAGKLFWALTDESNKEQADVIPVYAAAPASVVPDNASEPLAYAYKELT
                     PEIMRNHLAVFERYGIAPNDSSTTIQALRIALDGIERSDAMLHGAEPVSQTYKLPPLS
                     SNEVNDAAWKLHNMLTEHGPLNGRQFNNLKGCFYEALKVAMRNYPVTPDGWISCSERM
                     PEDTKMLLAFSQGQIVAAYWNWVMSPIDYKKYRAFTYLSGNILDDVTHWMPLLEPPEQ
                     DGE"
     gene            complement(1320..2006)
                     /locus_tag="OQM87_003294"
     CDS             complement(1320..2006)
                     /locus_tag="OQM87_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCS1509317.1"
                     /translation="MSEINYQALRKAAQLATQGEWVAFISSGTGTYAVHTPGDKRCED
                     VIKWTGFDGQNNAENNARYIAAFNPEVAQALLDERERNQQYIKRRDQENEDIALTVGK
                     LRVELEGKDSKIANLTAERDALREGEMGDARYSNTRAAADIYFQLVEECEIPAGGSLV
                     EYVDDMREKLESAEKRIAELEEREILLPERSSMLHRTDFHEDYQTVMAYKVSEVIDAI
                     RATGIRIKGE"
     gene            complement(1993..2307)
                     /locus_tag="OQM87_003295"
     CDS             complement(1993..2307)
                     /locus_tag="OQM87_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509318.1"
                     /translation="MTITKQRVEKIIYRHEMGLSSDVTAEEVYDLAVLALNLSNIANL
                     KRYELDMGGCDSCGQDCGADMTEDSDGDYVLFDDVVKLFEFDTTTQKLESPAKEATSE
                     RN"
     gene            complement(2304..2789)
                     /locus_tag="OQM87_003296"
     CDS             complement(2304..2789)
                     /locus_tag="OQM87_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509319.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVNETNAEYLVRKFAEAEAKCA
                     ALAAENAGLKAALNHEVIPDTAVEAFNETAIIDHDWNDTSEWSWVENDTDVIRAVLEA
                     IKPETPATDAFLAEIERKAIRKFINSIEHILRDKLSPYDTEEMLETMRIFLEEQGGEQ
                     K"
     gene            complement(2786..2950)
                     /locus_tag="OQM87_003297"
     CDS             complement(2786..2950)
                     /locus_tag="OQM87_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HCS1509320.1"
                     /translation="MRGLAYNLGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRNGKK"
     gene            complement(2961..3254)
                     /locus_tag="OQM87_003298"
     CDS             complement(2961..3254)
                     /locus_tag="OQM87_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HCS1509321.1"
                     /translation="MPAPQYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(3268..3774)
                     /gene="ssb"
                     /locus_tag="OQM87_003299"
     CDS             complement(3268..3774)
                     /gene="ssb"
                     /locus_tag="OQM87_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCS1509322.1"
                     /translation="MASRGVNKVIILGRVGQDPEVRYSPSGTAFANLTIATSEQWRDK
                     NTGEQKELTEWHRVAVSGKLAEVVGQYVKKGDQIYFEGMLRTRKWKDQSGQDRYTTEV
                     HVGINGVMQMLGGIGDSKQQAASRQSQKPQQQSSPAQHNEPPMDFDDDIPFAPVTLPF
                     PRHAIHAI"
     gene            complement(3775..4482)
                     /locus_tag="OQM87_003300"
     CDS             complement(3775..4482)
                     /locus_tag="OQM87_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCS1509323.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIRIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKHGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQRDWQLMAGNEEAQA
                     KCVQVMNIRVNELKQAA"
     gene            complement(4737..4889)
                     /gene="kil"
                     /locus_tag="OQM87_003301"
     CDS             complement(4737..4889)
                     /gene="kil"
                     /locus_tag="OQM87_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HCS1509324.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(4874..5005)
                     /locus_tag="OQM87_003302"
     CDS             complement(4874..5005)
                     /locus_tag="OQM87_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HCS1509325.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVDVLLCAMK"
     gene            complement(5030..5998)
                     /locus_tag="OQM87_003303"
     CDS             complement(5030..5998)
                     /locus_tag="OQM87_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HCS1509326.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVHDRLDELKAKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAKRERIEAEQRAQ
                     REAKEAAERAEREKQAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRIKDEEERRA
                     KDKAHRKEVNNKILADLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            complement(6166..6408)
                     /locus_tag="OQM87_003304"
     CDS             complement(6166..6408)
                     /locus_tag="OQM87_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509327.1"
                     /translation="MRVKTMGASLLSGRIFQGTLNTEKGMWVGKREDVTDQAVRAVAE
                     HMMIKDQKYAYETKDGKWLIISHQLVDKLPEEFVDG"
     gene            complement(6611..6943)
                     /locus_tag="OQM87_003305"
     CDS             complement(6611..6943)
                     /locus_tag="OQM87_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1509328.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRTERKNKRTWYSKPRSEIGVTCSGRQKQRG
                     KSIPAYYD"
     gene            complement(7404..7808)
                     /locus_tag="OQM87_003306"
     CDS             complement(7404..7808)
                     /locus_tag="OQM87_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509329.1"
                     /translation="MIRIAALLSILLTTSANSECWIVTNLHGYGSMNGDRYEFTKDST
                     EDSVFHVTINGDKSSVYESVSGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTDK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNQ"
     gene            complement(7805..8437)
                     /locus_tag="OQM87_003307"
     CDS             complement(7805..8437)
                     /locus_tag="OQM87_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCS1509330.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            8541..8756
                     /locus_tag="OQM87_003308"
     CDS             8541..8756
                     /locus_tag="OQM87_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1509331.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            8876..9169
                     /locus_tag="OQM87_003309"
     CDS             8876..9169
                     /locus_tag="OQM87_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HCS1509332.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            9202..9348
                     /locus_tag="OQM87_003310"
     CDS             9202..9348
                     /locus_tag="OQM87_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HCS1509333.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            9341..10174
                     /locus_tag="OQM87_003311"
     CDS             9341..10174
                     /locus_tag="OQM87_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HCS1509334.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     AAHLWVHLILKAKHTPEYVMTDAGEILVGRGKLLGGRNSLAFETGLKPDRVQYLLRKF
                     KKLGMIDWVSHGKFSVFSVEKYDDYQSNFVPADYQQITTDKEYNNIISNTDVLESTAA
                     DKKSDKKKPSVSCQDVVDAYHEILPEAPKIRALNDKRKNQIRTFWRKAGVITRQLDGH
                     GFTMQDWRNYLSYVGENCRWMFEERPNHQRGTVWHKKGFDFLLNDNTYLKVREGEHDD
                     R"
     gene            10164..11600
                     /locus_tag="OQM87_003312"
     CDS             10164..11600
                     /locus_tag="OQM87_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1509335.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKIIFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDLLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPSETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            11677..12117
                     /locus_tag="OQM87_003313"
     CDS             11677..12117
                     /locus_tag="OQM87_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCS1509336.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            12114..>12177
                     /locus_tag="OQM87_003314"
     CDS             12114..>12177
                     /locus_tag="OQM87_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCS1509337.1"
                     /translation="MRRQRRSFTDIICENCKYLPT"
BASE COUNT         2947 a         2992 c         2714 g         3524 t
ORIGIN      
        1 ttttcagtcc acatgcgatt gaggcaagag ttgcgctact gatgccaaca tcgccagtcg
       61 attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccaa taaaaaaccg
      121 ccatcaggcg gcatggtgtt ctttcagttc ctcaattcga atattggtta cgtctgcatg
      181 tgctatctgc gcccatatca tccagtggtt atagcagtcg ttgatgtcct ctgcttcgat
      241 aaccctgtcg aatggctctc cattccattc acctgtgact cggaagtgca tttatcatct
      301 ccataaaaca aaacccgccg tagcgagttc agataaaaga aatccccgcg agtgcgagga
      361 ttgttagttg ctatctgctg cttccttggt cattaccata tccacccaat ttcaccagac
      421 atgattctcg caatcactat tatcaccaaa gtgataatca caactttaac tggcggcatc
      481 attcaccatc ctgctccggc ggttctagca gcggcatcca gtgggttacg tcatccaaga
      541 tatttcctga taaatatgtg aaagctctat attttttgta atcaattgga ctcattaccc
      601 agttccaata tgctgccact atttgacctt gactaaatgc cagtaacatt ttggtgtctt
      661 ccggcattcg ctcactacag cttatccaac catccggagt taccggatag ttgcgcattg
      721 cgacctttaa tgcctcatag aagcaacctt tcagattgtt gaactgacgc ccatttagcg
      781 ggccatgttc agtcagcatg ttgtgtaatt tccatgccgc gtcgtttact tcgttggatg
      841 acaggggagg caacttgtaa gtttggctta caggttcggc accatgaagc atggcgtcgc
      901 tccgctctat gccatccagc gcgattcgta gtgcctgaat tgtggtagag ctatcgtttg
      961 gggctattcc atatcgctcg aatacagcta aatggttgcg cataatctca ggcgtaagct
     1021 ctttgtaagc ataagcgaga ggctctgatg cattatccgg cacaaccgac gcaggcgcgg
     1081 cagcataaac aggaataacg tccgcttgct ctttattgct ttcatccgtt aaagcccaga
     1141 ataatttccc ggccggatgt ttgaaaatat aagcaactgg ctctgcttcc agcgatgcca
     1201 gtgcaattcg tgccagttct tccgcttctt ctgctggcag tacaacgttg ctaccaggtc
     1261 cgtatgtttc gcgccactgc ttgattgtca gtagtcgctc tttggtaata gtggtcatct
     1321 cactctcctt tgatgcgaat accagtagcg cggattgcat cgatgacttc agaaactttg
     1381 tatgccatta ccgtttggta atcctcgtga aaatctgttc gatgaagcat gctgctacgt
     1441 tccgggagca gtatttcccg ctcctccagt tctgctatgc gcttctctgc ggattccagc
     1501 ttctcgcgca tatcgtcaac gtactcgacc agagaaccgc cagcaggaat ttcgcactcc
     1561 tcgaccagtt ggaagtagat atcagctgcg gcccgtgtgt tgctatacct agcgtcgccc
     1621 atctcacctt cacgaagagc atcgcgttcg gcggtaagat tggctatttt gctgtctttg
     1681 ccttccagct caacgcgcag cttccctacc gtaagcgcaa tatcctcgtt ctcctggtcg
     1741 cggcgtttga tgtattgctg gttcctttcc cgttcatcca gtagtgcctg cgctacttca
     1801 gggttgaaag ctgcgatata acgagcgttg ttctctgcgt tgttctgtcc atcaaagccg
     1861 gtccatttga taacgtcttc acatcgttta tcaccaggtg tatgcaccgc atatgtacca
     1921 gtaccagacg aaataaatgc gacccattca ccctgtgttg ccagttgcgc cgctttacgc
     1981 agtgcctggt aattaatttc gctcactggt tgcctccttt gctgggcttt ctaacttttg
     2041 agtggttgta tcaaattcaa acaacttaac cacgtcatca aacaggacat aatcaccatc
     2101 agaatcttca gtcatgtcag cgccacaatc ctgaccgcat gagtcacaac cacccatatc
     2161 aagctcgtat cgcttcaggt ttgcgatatt tgataaattc agcgccagta cagccaggtc
     2221 ataaacctct tcggcagtga catcgctgct cagtcccatt tcatggcgat atatgatttt
     2281 ttctactcgt tgttttgtga ttgtcatttt tgctcgcctc cctgttcttc cagaaaaata
     2341 cgcatagtct caagcatctc ttcggtgtca tacggtgaca acttgtcacg caggatgtgt
     2401 tcaatgctgt taatgaactt gcggattgct ttgcgttcaa tttcagccag aaaagcatcg
     2461 gttgccgggg tttcaggttt aatggcttca agcacggcgc gaatgacatc ggtatcgttc
     2521 tccacccatg accattcgct tgtatcattc cagtcgtggt ctatgatagc tgtttcattg
     2581 aatgcctcga ctgccgtatc cgggattact tcatgattta aagctgcctt cagccccgca
     2641 ttctccgccg ccagcgccgc gcacttggcc tccgcttcag caaatttacg caccagatat
     2701 tcagcgtttg tttcgttaac ctttaaatca cttgggatgc atttaccttt cagaaatcca
     2761 tccatctcaa ttagtgtcat ttgtttcatt tcttcccatt ccgccacatt gcattcagat
     2821 atttgttttg atttactgac ggaaaagaat ttcttttaag caattcctct ctcgatggca
     2881 ttggctttac gcgttggcga ataatcattt ctgccggaag aatgccgaga ttgtatgcaa
     2941 gtcctctcat ggtaaattcc tcagtcatta ctgatagcgc catagcgtga gcggtaatta
     3001 cgcaggcgcg ggtcaatttc agggaagtgg gtatatgtgg ctttgcggaa tggtcggatt
     3061 gatgtctggt aaattcgctc gcgctcttct ttctctgcaa gccatatgca gtggcgaaat
     3121 tccttttcct ctttcgtttc ctgcggtagc gacattatcc ggtcgtagtt tttcctgaat
     3181 ttatccagca cctccgatac ggaattgccg gaacagcggc gcgggtcatc cgcaccatac
     3241 tgaggcgctg gcatgatttt ctcctgatta aattgcgtga atagcgtgac gagggaaggg
     3301 gagagttact ggtgcaaagg gtatatcgtc gtcaaaatcc atcggaggtt cgttgtgttg
     3361 tgctggtgat gattgctgct gtggcttctg tgattgcctg ctggctgctt gttgtttgct
     3421 gtcgccaatg ccgccaagca tttgcatcac gccattaatt ccgacatgaa cctcggttgt
     3481 gtaacggtct tgccctgact ggtctttcca ctttctggtt ctcagcattc cctcgaaata
     3541 aatctgatca ccttttttca catactgccc cacgacctca gccagtttcc cggatacagc
     3601 aacacgatgc cattcagtca attccttttg ctcgccagta tttttatctc gccattgttc
     3661 tgacgtggct attgtcaggt tagcgaacgc tgttcctgat ggtgagtatc gaacttccgg
     3721 gtcttgtcct acccgaccaa ggataatcac cttatttacg cctctgcttg ccatttatgc
     3781 cgcctgtttt agttcgttaa ctctgatgtt cattacctga acgcatttag cctgcgcttc
     3841 ctcgttgcca gccattaatt gccagtcacg ctgataacgc tcgatgagtt ttttcttgtc
     3901 agtttctgtt gacgcataat cgctgaagtc tttcaggatt tgttcgcagt caaccgatgg
     3961 agatttctgg ttggtatttt ctggtgatgg tttgttatct gatgctggga ttgcccatcc
     4021 cggcagcgat ggagggagcc agtaaaatcc tgttccatcc ttcagttttg ccctgtgcca
     4081 cccatgcttt ttatcgagag atgtttgtgc gaaaccttcc tcaaggttat acagataccg
     4141 accgattccc cactgaacgg cagcacgctt cattgcacct gaacgaccac ctttgacggc
     4201 ttctacctgc gtgttttcag cagcatccca tttggttacc cattcggaat caatcctgat
     4261 tgatatgccg cattcaacgc ctccgttgtt gggaatatcg cggtattcat tgcgccatcc
     4321 tgctttgccg caaacatcgt ccaggcgttt catgattgcc cggttcgtga cataagccag
     4381 caccatagcc cacactttgc catcgcgtgt tttaccgctt tgctgtattc gccattcgat
     4441 atcttcaggg ctgaatggct catcgaattt attcaaatcc ataaatcacc tcagaatgga
     4501 catggcccaa gaaaataacg ctgatttaat acttcgactc gggacaaatt aaggcatacc
     4561 cgcattcctt cgcggtcgcc attatggcga taccagagag ctttctgggt gtacatgcgt
     4621 ctctgtaact tgctctcctt cactgtggtt gcaagtgaca tgaatatctc cttcgtttcc
     4681 gattaattct ttcatctgac gaatgaattc ttcgtctgac cagttatctg taaaactcat
     4741 ttcctgcgat accacggaag gttgatagct gacttcatcg ctttatttgc ttcaagccac
     4801 attttggaat caccaataaa tctggctatt actgctttgt tctgtgcagc acgaagcatc
     4861 tggtgattga tggctatttc attgcgcata ataagacatc aactcttttc catccgtcac
     4921 gtaatttacg ggtgattcgt tcaagtaaag attcatttaa ttggaaggca cccatgcgag
     4981 cgcctcccgc gattgcgtaa atcatgggtg gttccttatg ttggttttat tagtaggtta
     5041 tttttgttgc gaatacttcg ccttttacga tggctgttat gatattttta gcaacatctt
     5101 ctgatgcgcc aaccttgata aggtcagcaa gtattttgtt atttacttct tttcggtgag
     5161 ctttatcctt tgctctacgc tcttcttcgt ccttgattct tttttcttct gctattctgg
     5221 cttgctcttt tgcttcagct tcgcgccgga ttcgttcagc ctcctcctgt gcttttcggc
     5281 gttctgcttc aattgccgcc tgcttttctc tttcagctcg ttctgctgcc tcttttgctt
     5341 cgcgctgtgc tcgttgctcg gcttcaatgc gttcacgctt tgcacgttcc gctgcggcct
     5401 tagcttctgc ttctcgcctt gctgctgctt caatttcggc ttttgccttt gcttcggctt
     5461 ctgctctggc tttctcttca gcttctcttt ttaagcgttc ttcatgctct cgcttttcct
     5521 gctccgcttt gagtcttgcc tcttctcttt ggcggtcaaa ttcgcgatcc atcaaaatcg
     5581 ctatttcatg gtcagactca atttgctttg cgagagcttc agctgctgcc ttagcttctt
     5641 cttcggcttt aatccgcgcc tgttcctcct cataatcagt aagaggctgg cgcgccttgg
     5701 ctttcagctc atcaagtcga tcatgcactg ttttgcggtt ggcatcaatt agctttggaa
     5761 tttccttcag ttcagcaaca aggtctttgc caagaccatc gagatatgtt ttcgtctgcg
     5821 caactttata cgccagagaa gcgatctcct ttctgccctt tgccgttgtg atatcaggca
     5881 caaaggacat aacttcacgt tcaacctttt gaaggatttc ttcaatctgg tcggcagact
     5941 gaaatacagt cattgcattt gctttttcaa taacaactaa atctgttact tcactcatat
     6001 ttcccccgtc aaaaaaattg ccctcacact ggagggcaaa gaagatttcc aataatcaga
     6061 acaagtcggc tcctgtttag ttacgagcga cattgctccg tgtattcact cgttggaatg
     6121 aatacacagt gcagtgttta ttctgttgct tattccaaag caaatttaac catcaacaaa
     6181 ctcttctggt aatttatcaa ccagttgatg acttattatc agccatttgc catccttcgt
     6241 ttcgtatgca tatttctggt cttttatcat catgtgttcg gctactgccc tcactgcctg
     6301 atcggtgaca tcttctctct ttcctaccca cattcctttt tcagtgttta atgttccttg
     6361 aaaaatacga ccacttaata ggcttgcgcc catagttttt actctcatgt atcagtcctc
     6421 aaataagtgg tttgctgcga aagtaaatac gcttaagtta cctgttattt atcccaccaa
     6481 gttccgtatc tatctatcca gttacaccaa tcatcgacac tctattttgt tgtgtcgcat
     6541 tttggcaact ggcaagaata tctaccttct ttgtaaagtc ggcgtttgac tttcttgagc
     6601 atggctcacc tcaatcgtaa taagctggaa ttgattttcc gcgttgcttc tggcggcctg
     6661 agcaagtcac acctatttca ctgcgtggct tgctgtacca tgtgcgctta ttcttgcgct
     6721 cagtacgctg caggttgctt tcaatctgtt cgtggtattc agccagcacc gtaaggtcta
     6781 tcggattcag tgcgctttct actcgtgatt tcggtttgcg attcagcgag agaatagggc
     6841 ggttaactgg ttttgcgctt accccaacta acaggggatt tgctgctttc cattgagcct
     6901 gtttctctgc gcgacgttcg cggcggcgtg tttgtgcatc catctggatt ctcctgtcag
     6961 ttagctttgg tggtgtggtg gctggtagtc tagctccagc ttgttgagtc tcattcggag
     7021 gggtataacc ggcaccccag cgatttttcc atgcgacaac gtgcgcgtta tggcggcctt
     7081 atcgcccgcg gctccccatc tcgtccacgc tattgctagc gttgggagcg cttcaccgct
     7141 caacagtagg taagcacttg ccagtgacta gctggcttca ccacacccca aagccttctg
     7201 ctttgaatgc tgcccttctt cagggcttat tttttaagag cctcaccttc atggtggtca
     7261 gtgcgtcctg ctgatggctt aaaattacaa gaaagattgt atgttgtaaa caagaaatat
     7321 tgtgaaaagg ggcgtgaaaa acaaactcca ttgtttttaa atgaaaaata gtttgttttt
     7381 ggttatcgag attgaggtgg ggattactgg ttgcaggttc cgactacatc accaacaaat
     7441 gatttggttg atgtaagttg ttgcatacct gggatattca ttactttgga gtaaagagct
     7501 tttttgtctg tagtgattga ccaagtttca acggttattc ctcctccaga ctggtattct
     7561 cctaccatag tgttcgatga caaagcagtg tatttcatct ctggatagac gccagaaact
     7621 gattcataaa ctgatgattt atcgccattt attgttacgt ggaaaacgga atcttccgtg
     7681 ctgtcttttg taaactcgta acgatcgcca ttcattgacc cgtacccgtg caggtttgtg
     7741 acaatccagc attcagaatt ggcgctggta gttaagagta ttgagagtag cgccgcaatc
     7801 ctgatcatac gaattttacc ctcgcttcca cgacaacacc gataatcttg cagttcccgt
     7861 tgataggagt cataggccat gaagggttca ggcctttcag gtacttctgc ccgccatcta
     7921 taaccagttt cttgaatgtt gcttcgttcg cgtcagtcag tttggctaca acaaggcttc
     7981 cattcactgg ctcgcgtcca gtatctacta acaccatatg accttcaggg atgctttgac
     8041 ctacaggtga ggtcatggaa tcaccttcaa ccttcagcca gaatccatcg cctaataagt
     8101 taacgtcact gtcataccat tcatcaatgt ccttgatatc gtagggttca caagcttcac
     8161 accacgaacc agctctaacc atgctaatca atggatattt ccctttgggt tcaacatgcc
     8221 caacaaatct aacattcgaa tcagaggtgc cattgagcag ccagtcaaca cttacgccaa
     8281 gagctgacgc aagttctggt aaaaagcgtg gtcgcttagt tttaccgttt tcgagctgct
     8341 ctatagactg ctgggtagtc cccacctttt gagcaagttc agcctggtta agtccaagct
     8401 gaattctttt gctttttacc ctggaagaaa tactcataag ccacctctgt tatttacctc
     8461 caatcttcac aagaaaaact gtattttaca aacaagatac attgtatgaa aatacaagaa
     8521 agtttgttga tggaggcgat atgcaaactc tttctgaacg cctcaagaag aggcgaattg
     8581 cgttaaaaat gacgcaaacc gaactggcaa ccaaagccgg tgttaaacag caatcaattc
     8641 aactgattga agctggagta accaagcgac cgcgcttctt gtttgagatt gctatggcgc
     8701 ttaactgtga tccggtttgg ttacagtacg gaactaaacg cggaaaagcc gcttaagaca
     8761 ttcccgctct tacacatccc agtcctgaaa aagggcatca aattaaacca cacctatggt
     8821 gtatgcattt atttgcatac cttcaatcaa ttgttatcta aggaaatact tacatatggt
     8881 tcgtgcaaac aaacgcaacg aggctctacg aatcgagagt gcgttgctta acaaaatcgc
     8941 aatgcttgga actgagaaga cagcggaagc tgtgggagtt gataagtcgc agatcagcag
     9001 gtggaagagg gactggattc caaagttctc aatgctgctt gctgttcttg aatggggtgt
     9061 cgttgacgac gacatggctc gattggcacg acaagttgct tcgattctca ccaataaaaa
     9121 acgcccggct gcaaccgagc gttctgaaca aatccagatg gaattctgag gtcattactg
     9181 gatcaatcca caggagtaat tatgacaaaa caactcagtc cttaccagga caaaattcac
     9241 aaacacatac tacgtgatcg cttcctgtcc agcttcaagc agcctggtcg attccgggct
     9301 gagttggaaa aagtgaagct gatgcagaag gagaaaggtc atgagtaaca tatctaatct
     9361 agccgaagcc agagaggcca gaaggctaca acaaccgcat caaagcagcg gtaaggggta
     9421 tgccttgctg caccgtaaaa ttatggatgt gccgttttac aaggacgcag aagcagcgca
     9481 tctgtgggtt cacttaatcc tcaaagcaaa gcatacgcct gagtatgtaa tgactgacgc
     9541 aggagaaatt ctggtaggca gagggaagct acttggcggt agaaactctc tggcgtttga
     9601 aacaggactc aaaccagatc gcgttcagta cctgcttaga aagttcaaaa aactcggcat
     9661 gattgactgg gtttcacacg gtaaattctc agttttctcg gtagagaaat atgacgatta
     9721 tcagtcaaat tttgtaccag cagattacca gcaaattacc acagataaag aatataataa
     9781 tattatctct aatactgacg tattagagag taccgcagca gacaaaaagt ctgacaagaa
     9841 aaaaccttcc gttagctgtc aggatgttgt cgatgcttac cacgaaatcc ttcctgaagc
     9901 gccaaaaatc cgcgcactga atgacaagcg taaaaaccag atccgaacgt tctggcgcaa
     9961 agccggagtg ataacccgcc agcttgacgg gcatgggttc acgatgcagg actggagaaa
    10021 ttatttgagc tacgtaggcg aaaattgccg atggatgttc gaagagcgcc caaaccatca
    10081 gcgcggaacc gtctggcaca aaaagggatt tgatttcctg cttaacgata atacctacct
    10141 gaaagttcgt gagggtgaac acgatgaccg ataattttta tgcgccgccc catagcatcg
    10201 aggcagagca ggcggtgatt ggtggattgc ttctggatga tgacagcagt gagcgcgtcc
    10261 agaaggttct ggcgatgctg aagcctgatt cattttacag ccgaccacac aaaatcattt
    10321 tcgaagaaat aaccagaatg caccgggagc aaaagccagt agatggcctg acgcttttcg
    10381 atgaactgga gcgtaaatcg ttaacggcgt ctgttggcgg ttttgcttat atcgctgaga
    10441 tcgcaaagaa cacgccaagc gccgcaaaca tcgttgccta tgcaatgcag gttcgcgaaa
    10501 ccgcaatgga acgctacgcc atcaaccgca tgactgaagc gacggaattg ctctattccc
    10561 gcaacggaat gactgcgacg cagaagtacg aagctattca ggcgattttc acgcaactga
    10621 cagaccatgc aaaaacagga tcgcgtcgcg gccttcgctc atttggtgag gtcatggaag
    10681 actgggttag cgaccttgag aagcgatttg acccatcagg cgaacaacgg ggaatgagca
    10741 cagggatccc atcgctggac aggatgctgt caccgaaagg tctggtgaaa ggctctctgt
    10801 ttgtcattgg cgctcgacct aagatgggga aaacgacgct atacagccag atggcaatca
    10861 actgcgcagt gcatgagaaa aagcccgctc ttatgttcag ccttgaaatg ccaggtgacc
    10921 agatactgga aaaactggta gggcagaagt ctggtgttaa cccgaatatt ttttaccttc
    10981 cggcgacaaa tgacgctgat gacggctatc agggtgatta cgatggtgac ttcaacaggg
    11041 cgatcgaaac agccaatcgc ttgagtgaaa tcgacctgct ttacatcgac gacacgccgg
    11101 gattatctct ggctcaaatc gtcagcgaaa gccgtcgaat caagcgagaa aaaggatgtg
    11161 ttggcatgat tctggtcgat tacctgacac taatgaccgc tgaaaaggcc gatcgcaacg
    11221 accttgctta cggcatgatc accaaaggac tgaagaacct tgccaaagag cttgattgcg
    11281 ttgttgtgct tctgacacag cttaaccgcg cactggaaag ccgaaccaat aaacgcccat
    11341 taccaagtga ctcacgagat acagggcaga ttgaacagga ttgcgattat tgggtgggga
    11401 tccatcgtga aggtgctttt gatgacagtg ttccacctag tgaaaccgaa ctaatccttc
    11461 gtctcaatcg tcatggcaat accggcacgg tgtattgcat tcaggcaaat ggcgctattt
    11521 atgacacaga ccaacagtct gctgaaatgc gccgacgtga acgcgaggaa ccgcagtcca
    11581 agaagaaagg aggattctga tgaccatcta catcactgag ctaataacag gtctgctggt
    11641 aatcgcaggc ctttttattt gggggagagg gaagtcatga aaaagctaac ctttgaaatt
    11701 cgatctccag cacatcagca aaacgctatt cacgcagtac agcaaatcct tccagaccca
    11761 accaaaccaa tcgtagtaac cattcaggaa cgcaaccgca gcttagacca gaatcgaaag
    11821 ctttgggctt gccttggtga cgtctctcgt caggttgaat ggcatggtcg ctggctggat
    11881 gcagaaagct ggaagtgtgt gtttaccgca gccttaaagc agcaggacgt tgttcctaac
    11941 cttgccggga atggctttgt ggtaataggc cagtcaacca gcaggatgcg agtaagcgaa
    12001 tttgcggagc tattagagct tatacaggca ttcggtacag aacgtggcgt taagtggtca
    12061 gacgaagcgc gactggctct ggagtggaaa gcaagatggg gagacagggc ggcatgaggc
    12121 gacagcgacg aagtttcacc gacatcatct gcgaaaactg caaatacctt ccaacga
//
LOCUS       DAJVQH010000118        12155 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJVQH010000118 DAJVQH010000000
VERSION     DAJVQH010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.118"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            129..998
                     /locus_tag="OQM87_003315"
     CDS             129..998
                     /locus_tag="OQM87_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCS1509338.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALPLKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYARTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1201..1554)
                     /locus_tag="OQM87_003316"
     CDS             complement(1201..1554)
                     /locus_tag="OQM87_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCS1509339.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1692..3338)
                     /gene="groL"
                     /locus_tag="OQM87_003317"
     CDS             complement(1692..3338)
                     /gene="groL"
                     /locus_tag="OQM87_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCS1509340.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3382..3675)
                     /locus_tag="OQM87_003318"
     CDS             complement(3382..3675)
                     /locus_tag="OQM87_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCS1509341.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3951..5207
                     /gene="yjeH"
                     /locus_tag="OQM87_003319"
     CDS             3951..5207
                     /gene="yjeH"
                     /locus_tag="OQM87_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCS1509342.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5223..5699)
                     /gene="fxsA"
                     /locus_tag="OQM87_003320"
     CDS             complement(5223..5699)
                     /gene="fxsA"
                     /locus_tag="OQM87_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCS1509343.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6036..7472
                     /gene="aspA"
                     /locus_tag="OQM87_003321"
     CDS             6036..7472
                     /gene="aspA"
                     /locus_tag="OQM87_003321"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCS1509344.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7590..8891
                     /gene="dcuA"
                     /locus_tag="OQM87_003322"
     CDS             7590..8891
                     /gene="dcuA"
                     /locus_tag="OQM87_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCS1509345.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9007..9345
                     /gene="cutA"
                     /locus_tag="OQM87_003323"
     CDS             9007..9345
                     /gene="cutA"
                     /locus_tag="OQM87_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCS1509346.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9321..11018
                     /gene="dsbD"
                     /locus_tag="OQM87_003324"
     CDS             9321..11018
                     /gene="dsbD"
                     /locus_tag="OQM87_003324"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCS1509347.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11055..11630
                     /locus_tag="OQM87_003325"
     CDS             11055..11630
                     /locus_tag="OQM87_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1509348.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11737..11812
                     /locus_tag="OQM87_003326"
     tRNA            11737..11812
                     /locus_tag="OQM87_003326"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11770..11772,aa:Phe,seq:gaa)
BASE COUNT         2926 a         3138 c         2987 g         3104 t
ORIGIN      
        1 cttttctgtt tatatagagt gagcacggct atttatgtta ttttaacaac tattactacc
       61 aagcccattt atctggctta taaattaaaa ttaattattt aattgagaaa tggttaggga
      121 gaacctacat ggccataagc atcaagggtg ttaataccgg tgttattcgc aagagtaata
      181 atttcattgc actgccgctg aaaattaaag aacctcgcaa taaagaatcg ttgttcttta
      241 tgtctgtcat ggaactgcgc gacctgctga ttgcgctgga aagtcgtctg catcaaaagc
      301 ataaactgga tgctgctgca cgccttcagt atgagcaagc tcgcgataaa gtcattaaga
      361 aaatggcaga aaacatccca gaaattctgg ttgatgagct taaaaatgcc gatattaatc
      421 gtcgggttaa cacgctggaa cttaccgata atcaaggcga aaatcttacc ttcgtattaa
      481 cgctacatga cggtagcaaa tgtgagttag tcgtcaatga gttgcagatt gaaatgctgg
      541 cgcgagcaat cattcatgca attaataatg cggaaatgcg tgaactggca ttacgtatta
      601 cctcactgtt agatttcctg ccgttatacg acgttgactg ccaggacaat ggcaatctgg
      661 aatatgacac ctattcccaa ccggaatgga aacataactt gttcgaccat tatctggcgg
      721 tgctttaccg ctttaaagat gaaagtggca aagaacaatt tagcggcgca gtggtgaaaa
      781 cgcgtgaagc aacgccgggt aaagaaatag aggcgatcac tcgccgaatg cttgatttca
      841 gcccacgact gaaaaaactc gcgggtgtgc catgtcaggt ctatgctcgc accgtagcgg
      901 cgaataacgc ccaaccgcta acccaggatc aatgcttacg cgcgctgcat cacttacgtg
      961 tacaatccac cagcaaaact gcaccacagg cgaaataatc tcatctttgc taatgcgagg
     1021 ctggagccat ccagcctgaa atgactgata acgtgatcgt tggtcatgcc ggatgcggcg
     1081 taaacgcctt atccggccta caaattctca agcattcaat cagttgcaat gcctgctgta
     1141 ggcctgataa gcgaagcgca tcaggcaata atacgtttgt cacccagcct ctggatcaca
     1201 tcagttcggg cacttataaa cctgaccgat aatctggcta tccgtcggga caaaactgga
     1261 caacattccc tgcgacgggc tgctgatgcc ataaatcacg ttaccgccca ttgcagccgc
     1321 ctggttgcgc agatcgtttg ctgcgccgcg catagaaccg ccctcttctc cgtgttgccc
     1381 ggaaagccag ttgctttgct tacctgtcgc agtaccaatc agctggcact ctgcgcctgg
     1441 ctgctcgtcc acaatgcgta cactctgacc ggcagcactc aattcgttgc tggagctaca
     1501 acccgccatc agtagcgcgg caccgacaat ccctgctaag tattttacgt gcatgttatt
     1561 ccccataatc aatgagctgg acgccactcg cgtcgtccgt gtctgaatct tatactagaa
     1621 agatgaccaa aagaaaaacc cccagacatt tctgcccggg ggtttgttta tttctgcgaa
     1681 gtgcagggca attacatcat gccgcccatg ccacccatgc cgcccatacc gccagcagcg
     1741 cctaagtcag ctgcatcgtt tttcggcagg tcggtaacca tgcattcggt ggtgatcatc
     1801 aggccagcca cagaagctgc gtactgcaga gcagaacggg ttactttggt tgggtccagg
     1861 atacccatgt cgatcatgtt gccgtattct tcggttgctg cgttgtaacc gtagttgccg
     1921 tcgccgcctt taacggtgtt agcaacaaca gacggttctt cgccgcagtt caggacgatc
     1981 tgacgcagcg gagcttccat tgcacgcagt gcaactttga tacccacgtt ctggtcttcg
     2041 ttctgaccac gcaggtcagc cagtttagac gctacgcgga tcagcgcaac accaccacca
     2101 gcaaccacgc cttcttctac cgcagcacgg gtcgcgtgca gggcatcttc aacgcgtgct
     2161 tttttctctt tcatttcaac ttcggtagca gcacctactt tgataactgc aacgccgcct
     2221 gccagtttcg ctacgcgctc ctgcagtttt tcacggtcgt agtcagaagt tgcttcttca
     2281 atctgctgac ggatctgagc aacacggccc tggattgcag cttcttcacc cacgccatcg
     2341 atgatagtgg tggtgtcttt gttgatcaca acacgtttag cctgacccag gtcttccagg
     2401 gttgcttttt ccagctccat accgatctct tcagagatta cggtaccgcc agtcagggtt
     2461 gcgatatcct gcagcatagc tttacgacga tcgccgaagc ccggagcttt aaccgcagca
     2521 actttcacga tgccacgcat ggtgttaaca accagagttg ctagcgcttc gccttcaaca
     2581 tcttcagcga tgatcagcag cggtttgcct gctttggcaa cagcttccag aaccggcagc
     2641 atttcgcgga tgttggagat tttcttgtca gccagcagga tgaacgggct ttccagttct
     2701 actgcgccag tttccggctt gttgatgaag taaggagaca ggtagccacg gtcgaactgc
     2761 ataccttcaa ccacgtccag ttcgtcctgc agaccggtac cgtcttcaac ggtgataacg
     2821 ccttctttac cgactttgtc catcgcttca gcgatcagtt tacctacggt ttcgtcggag
     2881 ttagcggaga tggtaccaac ctgagcaatc gctttagaat cagagcacgg tacggacagc
     2941 gctttcagtt cttcaactgc agcggtaacc gctttgtcga taccacgttt caggtccatc
     3001 gggttcatgc ccgcagcaac agctttcaga ccttcagtga tgatagcctg agccagtacg
     3061 gttgcagtgg tggtaccgtc gcctgcagcg tcgttcgctt tggaggcaac ttctttcacc
     3121 atctgcgcac ccatattttc gaacttgtct tccagttcga tttcacgagc aacggaaaca
     3181 ccatctttgg tgatggtcgg tgcaccgaaa gatttatcca gaactacgtt acggcctttt
     3241 ggaccgaggg taactttcac tgcatctgcc agtacgttta cgccgcgcag cattttcaca
     3301 cgagcgtcgt taccgaattt tacgtcttta gctgccatta tctttattcc ttaaattcgt
     3361 atgttcagtg tcgtgcgcgg attacgcttc aacaattgcc agaatgtcgc tttcggacat
     3421 gatcaacact tcttcattgt cgatcttctc ggatttcaca ccgtagccat cgttgaaaat
     3481 aacgatgtcg ccaactttca cgtccagcgg cttcacttcg ccattttcaa ggatacggcc
     3541 attgccgaca gccagcactt cgccgcgggt ggatttagcc gctgcagagc cggtcagaac
     3601 gatgccgcca gcagatttag tttcaacttc tttacgcttg acgatcacgc gatcatgcaa
     3661 tggacgaata ttcattgata actctccttt gagaaagtcc gtatctgtta tgggtgacgc
     3721 cggagcttac gtggtttccc ggctggtgac cagagaaatg gggatgaggc ttcgcccctt
     3781 caagggggaa aaagaaaaaa aattctgaac agcacaaaat cgggtgaaaa ccctgattca
     3841 cctcacattt catcgcaatt tcttcatcgc cgtcataagc gaatctgatt gtgctaccat
     3901 cgaaaatcta cgcagttgcc caaaatttgg gcgcaatcgg atatcaaccc atgagtggac
     3961 tcaaacaaga actggggctg gcccagggca ttggcctgct atcgacgtca ttattaggca
     4021 ctggcgtgtt tgccgttcct gcgttagctg cactggtagc aggcaataac agcctgtggg
     4081 cgtggcccgt tttgattatc ttagtgttcc cgattgcgat tgtgtttgcg attctgggtc
     4141 gccactatcc cagcgcaggc ggcgtcgcgc acttcgtcgg tatggcgttt ggttcgcggc
     4201 ttgagcgagt caccggctgg ctgtttttat cggtcattcc cgtgggtttg cctgccgcac
     4261 tacaaattgc cgccggattc ggccaggcaa tgtttggctg gcatagctgg caactgttgt
     4321 tggcagaact cggtacgctg gcgttggtgt ggtatatcgg tactctcggt gccagttcca
     4381 gtgctaatct acaaaccgtt attgccggac ttatcgtcgc actgattgtc gctatctggt
     4441 gggcgggcga tatcaaacct gcgagtatcc ccttccccgc gccaggaaat atcgaactta
     4501 ccgggttatt tgctgcgtta tcagtgatgt tctggtgttt tgtcggtctg gaagcatttg
     4561 cccatcttgc ctcggaattt aaaaatccag agcgtgattt tcctcgtgct ttgatgattg
     4621 gtctgctgct ggcaggatta gtctactggg gctgtacggt agtcgtctta cacttcgacg
     4681 cctatggtga acaaatggcg gcggcagcat cgcttccaaa aattgtagtg cagttgttcg
     4741 gtgtaggagc gttatggatt gcctgcgtaa ttggctatct ggcctgcttt gccagtctta
     4801 acatttatat acagagcttc gcccgcctgg tctggtcgca ggcgcaacat aatcctgacc
     4861 actacctggc acgcctctct tctcgccata tcccgaataa tgccctcaat gcggtgcttg
     4921 gctgctgcgt ggtgagcacg ttggtaattc atgccttaga gatcaatctg gaggctctta
     4981 ttatttatgc caatggcatc tttattatga tttatctgtt atgcatgctg gcaggctgta
     5041 aattattgca aggtcgttat cgactactgg cggtggttgg cgggctgtta tgcgttctgt
     5101 tactggcaat ggtcggctgg aaaagtctct atgcgctgat catgctggtg gggttatggc
     5161 tgtttctgcc aaaacgaaaa acgccggaaa atggcataac cacataatcc ggcgtttcga
     5221 cattaatcct ggcgatcgtc tttatgatca aggcggtcgc ggtcatcatc ctttcgctga
     5281 tactcaccat caaaagtatt accgccaccg gtcccggcgc taaaaccgcc gcctggcatg
     5341 cgagaaaagc gcaaatgtgg catcaacttc actgtcagat gcttttgcac cggcggcaat
     5401 aaaagcagaa gaccgaggaa gtcggtgaaa aagcccggta ataaaagcag caaaccggca
     5461 ataatcagcg aaacactttt aatcatctcc gccgcagggt tttcacccgc cgccattttt
     5521 tgctgcatca gcacgaaatt cttaaagccc tggttacgta ccagtgacat accgataact
     5581 gacgtgaata taaccagcac gagggtcagc aataccccca atacatgggc aacctgaata
     5641 aagattgaaa tctcaatata gacataaagg aaaatggcaa taaaaggtaa ccagcgcaaa
     5701 ggtttctcct gtaatagcag ccggttaaac ccggctacct gaatgggttg cgaatcgcgt
     5761 tcagcttata ttgtggtcat tagcaaaatt tcaagatgtt tgcgcaacta tttttggtag
     5821 taatcccaaa gcggtgatct atttcacaaa ttaataatta aggggtaaaa accgacactt
     5881 aaagtgatcc agattacggt agaaatcctc aagcagcata tgatctcggg tattcggtcg
     5941 atgcagggga taatcgtcgg tcgaaaaaca ttcgaaacca catatattct gtgtgtttaa
     6001 agcaaatcat tggcagcttg aaaaagaagg ttcacatgtc aaacaacatt cgtatcgaag
     6061 aagatctgtt gggtaccagg gaagttccag ctgatgccta ctatggtgtt cacactctga
     6121 gagcgattga aaacttctat atcagcaaca acaaaatcag tgatattcct gaatttgttc
     6181 gcggtatggt aatggttaaa aaagccgcag ctatggcaaa caaagagctg caaaccattc
     6241 ctaaaagtgt agcgaatgcc atcattgccg catgtgatga agtcctgaac aacggaaaat
     6301 gcatggatca gttcccggta gacgtctacc agggcggcgc aggtacttcc gtaaacatga
     6361 acaccaacga agtgctggcc aatatcggtc tggaactgat gggtcaccag aaaggtgaat
     6421 atcagtacct gaacccgaac gaccatgtta acaaatgtca gtccactaac gacgcctacc
     6481 cgaccggttt ccgtatcgca gtttactctt ccctgattaa gctggtagat gcgattaacc
     6541 aactgcgtga aggctttgaa cgtaaggctg tcgaattcca ggacatcctg aaaatgggtc
     6601 gtacccagct gcaggacgca gtaccgatga ccctcggtca ggaattccgc gctttcagca
     6661 tcctgctgaa agaagaagtg aaaaacatcc aacgtaccgc tgaactgctg ctggaagtta
     6721 accttggcgc aacagcaatc ggtactggtc tgaacacgcc gaaagagtac tctccgctgg
     6781 cagtgaaaaa actggctgaa gtcactggct tcccatgcgt accggctgaa gacctgatcg
     6841 aagcgacctc tgattgcggc gcttatgtta tggttcacgg cgcgctgaaa cgcctggctg
     6901 tgaagatgtc caaaatctgt aacgacctgc gcttgctctc ttctggtcca cgtgccggcc
     6961 tgaacgagat caacctgccg gaactgcagg cgggctcttc catcatgcca gctaaagtaa
     7021 acccggttgt tccggaagtg gttaaccagg tatgcttcaa agtcatcggt aacgacacca
     7081 ctgttaccat ggcagcagaa gcaggtcagc tgcagttgaa cgttatggaa ccggtcattg
     7141 gccaggccat gttcgaatcc gttcacattc tgaccaacgc ttgctacaac ctgctggaaa
     7201 aatgcattaa cggcatcact gctaacaaag aagtgtgcga aggttacgtt tacaactcta
     7261 tcggtatcgt tacttacctg aacccgttca tcggtcacca caacggtgac atcgtgggta
     7321 aaatctgtgc cgaaaccggt aagagtgtac gtgaagtcgt tctggaacgc ggtctgttga
     7381 ctgaagcgga acttgacgat attttctccg tacagaatct gatgcacccg gcttacaaag
     7441 caaaacgcta tactgatgaa agcgaacagt aatcgtacag ggtagtacaa ataaaaaagg
     7501 cacgtcagat gacgtgcctt ttttcttgtg agcagtaact taaaaataac aatctaatat
     7561 caacttgtta aaaaacaagg aaggctaata tgctagttgt agaactcatc atagttttgc
     7621 tggcgatttt cttgggcgcc agattggggg gaataggtat tggttttgca ggcggattgg
     7681 gggtgctggt tcttgccgct attggcgtta aacccggtaa catcccgttc gatgtcattt
     7741 ccattatcat ggcggttatc gccgctattt ctgccatgca ggttgctggc ggtctggact
     7801 atctggttca tcagacagaa aagctgctgc gccgtaaccc gaaatacatc acgatcctcg
     7861 caccgatcgt gacctatttc ctgactatct ttgctggtac tggcaacatc tctctggcga
     7921 cactgccagt tatcgctgaa gttgcgaagg aacaaggcgt taaaccttgc cgtccgctct
     7981 ctactgcagt ggtatccgcg cagattgcga tcaccgcatc gccaatctca gcggcagtgg
     8041 tttacatgtc ttccgtgatg gaaggtcatg gcatcagcta cctccatctg ctctccgtgg
     8101 tcatcccgtc caccctgctg gcggttctgg tgatgtcctt cctggtcact atgctgttca
     8161 actccaaact ctctgacgat ccgatttatc gcaagcgtct ggaagagggc ttggttgaac
     8221 tgcgcggtga aaagcagatt gaaatcaaat ccggtgcaaa aacgtccgtc tggctgttcc
     8281 tgctgggcgt agttggcgtg gttatctatg caatcatcaa cagcccaagc atgggtctgg
     8341 ttgaaaaacc gctgatgaac accaccaacg caatcctgat catcatgctc agcgttgcaa
     8401 ctctgaccac cgttatctgt aaagtcgata ccgacaacat cctcaactcc agcaccttca
     8461 aagcaggtat gagcgcctgt atttgtatcc tgggtgttgc gtggctgggc gatactttcg
     8521 tttccaacaa catcgactgg atcaaagata ccgctggtga agtgattcag ggtcatccgt
     8581 ggctgctggc cgtcatcttc ttctttgctt ctgctctgct gtactctcag gctgcaaccg
     8641 caaaagcact gatgccgatg gctctggcac tgaacgtttc accgctgacc gctgttgctt
     8701 ctttcgctgc ggtgtctggt ctgttcattc tgccgaccta cccgacgctg gttgctgcgg
     8761 tacagatgga tgacacgggt actacccgta tcggtaaatt cgtcttcaac catccgttct
     8821 tcatcccggg tactctgggt gttgccctgg ccgtttgctt cggcttcgtg ctgggtagct
     8881 tcatgctgta atgatcaatc gcggggcgtt cacgccccgc tttctttccc gccgactaac
     8941 atccttcccc cgtccgttgt atagtgacct ctctcttgcg gttccatctg ttcttgcgag
     9001 gtgtttatgc ttgatgaaaa aagttcgaat accgcgtctg tcgtggtgct atgtaccgca
     9061 ccggatgaag cgacagccca ggatttagcc gccaaagtgc tggcggaaaa actggcggcc
     9121 tgcgcgacct tgatccccgg cgctacctct ctctattact gggaaggtaa gctggagcaa
     9181 gaatacgaag tgcagatgat tttaaaaact accgtatctc accagcaggc actgctggaa
     9241 tgcctgaagt ctcatcatcc atatcaaacc ccggaacttc tggttttacc tgttacacac
     9301 ggagacacag attacctctc atggctcaac gcatctttac gctgattctg ctactttgca
     9361 gcacttccgt ttttgccgga ttattcgacg cgccgggacg ttcacaattt gtccccgcgg
     9421 atcaagcctt tgcttttgat tttcagcaaa accaacatga ccttaatctg acctggcaga
     9481 tcaaagacgg ttactacctc taccgtaaac agatccgcat tacgccggaa cacgcgaaaa
     9541 ttgccgacgt gcagctgccg caaggcgtct ggcatgaaga tgagttttac ggcaaaagcg
     9601 agatttaccg cgatcggctg acgcttcccg taaccatcaa ccaggcgagt gcgggagcga
     9661 cgttaactgt cacctaccag ggctgtgctg atgccggttt ctgttatccg ccagaaacca
     9721 aaaccgttcc gttaagcgaa gtggtcgcca acaacgcagc gtcacagcct gtgtctgttt
     9781 cgcagcaaga gcagcacacc gcgcaattgc ccttttccgc gctctgggcg ttgttgatcg
     9841 gtattggtat cgcctttacg ccatgcgtgc tgccaatgta cccactgatt tctggcatcg
     9901 tgctgggtgg taaacagcgg ctctccactg ccagagcatt gttgctgacc tttatttatg
     9961 tgcaggggat ggcactgact tacacggcgc tgggtctggt agttgccgcc gcagggttac
    10021 agttccaggc ggcgctacag cacccatacg tgctcattgg cctcgccatc gtctttacct
    10081 tgctggcgat gtcaatgttt ggcttgttta ctctacaact cccctcttcg ctgcaaacac
    10141 gtctcacgtt gatgagcaat cgccaacagg gcggctcacc tggcggtgtg tttgttatgg
    10201 gggcgattgc cggactgatc tgttcaccat gcaccaccgc accgcttagc gcgattctgc
    10261 tgtatatcgc ccaaagcggg aacatgtggc tgggcggcgg cacgctttat ctctatgcgt
    10321 tgggcatggg cctgccgctg atgctaatta ccgtctttgg taaccgcttg ctgccgaaaa
    10381 gcggcccgtg gatggaacaa gtcaaaaccg cgtttggttt tgtgatcctc gcactgccgg
    10441 tcttcctgct ggagcgagtg attggtgatg tatggggatt acgcttgtgg tcggcgctgg
    10501 gtgtcgcatt ctttggctgg gcctttatca ccagcctaca ggctaaacgc ggctggatgc
    10561 gcgtggtgca aataatcctg ctggcagcgg cattggttag cgtgcgccca cttcaggatt
    10621 gggcatttgg tgcgacgcat accgcgcaaa ctcagacgca tctcaacttt acacaaatca
    10681 aaacgataga tgagttaaat caggcgctcg ttgaagccaa aggcaaaccg gtgatgttag
    10741 atctttatgc cgactggtgc gtcgcctgta aagagtttga gaaatacacc ttcagcgacc
    10801 cgcaggtgca aaaagcgtta gcagacacgg tcttacttca ggccaacgtc acggccaacg
    10861 acgcacaaga tgtggcgctg ttaaagcatc ttaatgtcct tggcctaccg acaattctct
    10921 tttttgacgg acaaggccag gagcatccac aagcacgcgt cacgggcttt atggatgctg
    10981 aaaccttcag cgcacatttg cgcgatcgcc aaccgtgaac gacacttgca gtgggaaaga
    11041 cggaggaaaa tagcgtgcaa cgtgaagatg tactgggaga agccctgaaa ttattagaat
    11101 tacaagggat tgccaacacc acgctggaga tggttgctga acgtgtggat tatccactgg
    11161 acgagctacg ccgcttctgg ccagacaaag aggcaatcct ctacgatgcg ctgcgttatc
    11221 ttagccaaca gatagatgtc tggcgtcgtc agctgatgct ggacgaaacg caaaccgccg
    11281 aacaaaagct gctggcacgt tatcaggcgt tatcggagtg cgttaaaaac aaccgctatc
    11341 cgggctgtct atttatcgct gcctgtacgt tttatcccga tcctggccac cctattcatc
    11401 aactggccga tcagcaaaaa agcgcggcct acgatttcac ccacgaactg ttaaccacgc
    11461 tggaagttga cgatccggcg atggtagcaa agcagatgga actggtgctg gaaggctgtt
    11521 taagccgaat gctggtgaat cgtagccagg cggatgtcga cactgcacat cggctggcgg
    11581 aagatatcct gcgcttcgcc cgctgccgcc agggtggtgc attgacctga cagaaacaca
    11641 gaaaagaagc gatttgccgc aatcttaagc agttgaatcg cttttactga aattaggttg
    11701 acgagatgtg cagattacgg tttaatgcgc cccgttgccc ggatagctca gtcggtagag
    11761 caggggattg aaaatccccg tgtccttggt tcgattccga gtccgggcac caaattcata
    11821 tcaacggacc tccacggagg tccgtttttc gtttcaggac gccacgattt aagcgttctg
    11881 cctccaaatc aattctaccg aactcaacca gattctcccc acatcaccag caatttgcga
    11941 gcatatccca atttgggaaa atttgttcct gagctatagc actaactgac gtgaaatgtc
    12001 gtgcggcccc gtgatgctgt tgaacgtcaa atgacgtcat caggagcgta acgtatccat
    12061 aaagcacaac accggtctga acgccgactg atgagatttt ctgaatgaga acaaagagaa
    12121 atgtgtcagt ccgcttgctc atgcaaagac taaca
//
LOCUS       DAJVQH010000119        11931 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJVQH010000119 DAJVQH010000000
VERSION     DAJVQH010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.119"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(1..2252)
                     /locus_tag="OQM87_003327"
                     /pseudo
     CDS             complement(1..2252)
                     /locus_tag="OQM87_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            complement(2276..2899)
                     /locus_tag="OQM87_003328"
     CDS             complement(2276..2899)
                     /locus_tag="OQM87_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCS1509349.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVINH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="OQM87_003329"
     CDS             complement(2896..4584)
                     /locus_tag="OQM87_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCS1509350.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAVGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OQM87_003330"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OQM87_003330"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCS1509351.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYGTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="OQM87_003331"
     CDS             7507..8229
                     /locus_tag="OQM87_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCS1509352.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..11931)
                     /gene="yfaL"
                     /locus_tag="OQM87_003332"
                     /pseudo
     CDS             complement(8357..11931)
                     /gene="yfaL"
                     /locus_tag="OQM87_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2768 a         3310 c         2939 g         2914 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg aacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggagctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacataa
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagaccg tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttatcc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc taggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatacag gcgaacgtcc acgccgccgt actcttccat ctgataacgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggttgatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc acggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctgg ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaactg
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatctgca tgattctgat aatagaaaac
     4321 gaaatcctcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctgatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg ccataaaccg ccaagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccatga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag agacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata ataattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccggcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcacccgcta aacaagccgc
     9061 cgctaagcag caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcacc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga aggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccctcacgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctttg ccatcattag cgatattatt gcgaaacatg gcgttagtga
    11641 cacgtaaatc gacggcacca gtatcattag taccagaaga atagattgcg ccactatagc
    11701 cgcctgcgac gttaccggaa aaaataacat cagtaagatt cagcgttgag ttttctttag
    11761 caaatatggc cccgccgtta ttatattctc ctgtaacggt gttattggca aacagagtca
    11821 ttccagtttc attttctggt aatagtgaaa attccgctcc ttgttgcaaa aatacggccc
    11881 caccgctggc attattggtc atatccgaaa aaaccagcca ttgcccatcg g
//
LOCUS       DAJVQH010000120        11848 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJVQH010000120 DAJVQH010000000
VERSION     DAJVQH010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.120"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..1019
                     /locus_tag="OQM87_003333"
     CDS             <1..1019
                     /locus_tag="OQM87_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1509353.1"
                     /translation="ELEEKWLDLKRMEISANAFNRYESVARTMVPKIGGGRLVSSVTK
                     EELLYIRKDLLTGYQNSTKNKAAAKGRSVVTVNYYMTTIAGMFQFAADHGYLEANPFQ
                     GIKPLKRARAEPDPLTRDEFIRLIDACRHQQTKNLWSLAVYTGMRHGELVSLAWEDID
                     MKAGTITIRRNYTKLGEFTLPKTEASTNRVVHLIQPAISVLKNQAEMTRLGKQHHIKV
                     QLREYGRSVNHECTFVFNPQVVRKSKQVGFIYKVDSIGDSWETAIKRAGIRHRKAYQS
                     RHTYACWSLSAGANPSFIASQMGHASAQMVFNVYGAWMTDSNAEQIAMLNQKLADYVP
                     WSPK"
     gene            1032..1802
                     /locus_tag="OQM87_003334"
     CDS             1032..1802
                     /locus_tag="OQM87_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCS1509354.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            1855..2250
                     /locus_tag="OQM87_003335"
     CDS             1855..2250
                     /locus_tag="OQM87_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCS1509355.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2291..3034
                     /gene="cmoA"
                     /locus_tag="OQM87_003336"
     CDS             2291..3034
                     /gene="cmoA"
                     /locus_tag="OQM87_003336"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCS1509356.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3031..4002
                     /gene="cmoB"
                     /locus_tag="OQM87_003337"
     CDS             3031..4002
                     /gene="cmoB"
                     /locus_tag="OQM87_003337"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCS1509357.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4167..6596)
                     /gene="torZ"
                     /locus_tag="OQM87_003338"
     CDS             complement(4167..6596)
                     /gene="torZ"
                     /locus_tag="OQM87_003338"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCS1509358.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPMRVGGVLPEMSAAIAGQASEAADDGGMAAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6621..7721)
                     /locus_tag="OQM87_003339"
     CDS             complement(6621..7721)
                     /locus_tag="OQM87_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCS1509359.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8109..8855)
                     /gene="cutC"
                     /locus_tag="OQM87_003340"
     CDS             complement(8109..8855)
                     /gene="cutC"
                     /locus_tag="OQM87_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCS1509360.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(8869..9435)
                     /locus_tag="OQM87_003341"
     CDS             complement(8869..9435)
                     /locus_tag="OQM87_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1509361.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9651..11384
                     /gene="argS"
                     /locus_tag="OQM87_003342"
     CDS             9651..11384
                     /gene="argS"
                     /locus_tag="OQM87_003342"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCS1509362.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            11561..>11848
                     /locus_tag="OQM87_003343"
     CDS             11561..>11848
                     /locus_tag="OQM87_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HCS1509363.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDTQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKK"
BASE COUNT         2940 a         3005 c         2866 g         3037 t
ORIGIN      
        1 aggaacttga agaaaagtgg ctggatctga aacgaatgga aatctctgca aatgcattca
       61 atcgctatga atccgttgca agaacgatgg ttccgaaaat tggaggcggc agactggtgt
      121 catcggtaac caaagaggaa ttgctgtata tcaggaaaga tttgctgacc gggtatcaga
      181 attcaacgaa aaacaaagca gcagcaaaag gacggagcgt cgttactgta aattattaca
      241 tgacgacaat cgctggaatg tttcagtttg ctgcagatca cggttactta gaagcaaatc
      301 ccttccaggg aattaagcct cttaaaagag ccagggcaga gccagatccg ttaactcgtg
      361 acgaatttat tcgcctgata gatgcttgcc gacatcagca gacgaaaaac ctgtggtcat
      421 tggctgtgta cacaggaatg cgtcacggtg aactggtctc cctggcctgg gaagatatcg
      481 atatgaaagc aggaactatt actatcaggc gcaattatac gaaactcggt gagttcactc
      541 tacctaaaac tgaagcaagt acaaacaggg ttgtgcatct tatccagccc gctatcagtg
      601 tcctgaaaaa tcaggctgaa atgacaagac tgggtaagca gcaccacatc aaggttcaac
      661 tacgtgaata tggacgttca gtgaatcatg aatgtacttt cgtatttaac ccccaggtgg
      721 ttagaaaaag caaacaggta ggttttatct acaaggtaga ttccattggc gactcatggg
      781 aaacagccat taagcgtgcg ggcatcaggc accggaaagc ataccagtca cgacacactt
      841 atgcgtgctg gtcattatct gccggagcaa acccaagctt cattgccagc cagatgggcc
      901 atgcaagtgc ccagatggtg ttcaatgtat acggagcatg gatgactgac agtaatgcag
      961 aacagatcgc aatgctgaat cagaagctgg cagattatgt cccatggtca ccaaagtgac
     1021 acaagagact tattaaaatc agtgggttag ccttcaatac ccgtcatgtt aactgcgtgg
     1081 agggcaacac cacgctttac gccctgccga aacccgaggt tgtcctgcgc tggcgtgaac
     1141 agaccacaga tgacttccgc ttctgtttta agtttccggc gaccatttcg catcaggcag
     1201 cattacgaca ttgcgatgat ttagtgactg aatttttgac ccgcatgtca ccgttggctc
     1261 cgcgcattgg gcaatactgg ctgcaactgc ctgccacatt cggcccacgg gagctgcctg
     1321 cgctttggca ttttctcgat tctctccctg gcgaatttaa ttatggtgtg gaagtccgcc
     1381 atccacagtt tttcgccaaa ggggaagagg aacaaacgct taatcgcggt ttacatcagc
     1441 gcggcgttaa tcgggtgatt ttagacagcc gcccggttca tgcatcacgt ccacacagtg
     1501 aagctattcg cgacgctcaa cgaaaaaaac ctaaagttcc ggtacatgct gtactgacgg
     1561 cgacaaatcc gctgatccgt tttatcggta gtgatgatat gacgcaaaac cgggaattat
     1621 ttcaggtctg gttacaaaaa ttagcgcagt ggcatcagac cactacgcct tatctttttt
     1681 tacatacgcc agatattgcc caggccccgg aactggtaca taccctgtgg gaagacttac
     1741 gtaaaacgct tccagagatc ggagcagttc cggctattcc acagcaatct tctcttttct
     1801 gaatttgcca cctatcatag acaggtgcca tcggccattt taaagggagt ttgtatggta
     1861 agcgcgctat atgccgtttt aagtgcgttg ttattaatga agttctcttt tgatgtcgtt
     1921 cgcctgcgaa tgcagtaccg cgttgcctat ggtgacggtg gttttagcga actgcaaagc
     1981 gctattcgca ttcatggtaa cgcggtggaa tatattccca tcgcgattgt gctgatgctg
     2041 tttatggaaa tgaatggcgc agaaacctgg atggtgcata tttgcggcat cgttttgctt
     2101 gctggtcgtc tgatgcatta ttacggtttt catcaccgtc tgtttcgctg gcgacgttcc
     2161 ggcatgagcg ccacctggtg tgcgctgttg ctgatggtgc tggcgaatct ttggtatatg
     2221 ccctgggagt tggttttctc cctgcgttag cgcacaatac gccactttct ttttcccgga
     2281 tttttacgtt atgtctcacc gcgacacgct attttctgcc cctatcgcca gactgggcga
     2341 ctggaccttt gatgaacggg tagctgaagt cttcccggat atgatccagc gttccgttcc
     2401 cggctattcc aatattattt ccatgattgg tatgttagcc gagcgcttcg ttcaacctgg
     2461 tacgcaggtt tacgatctgg gttgttctct gggcgcggcg acgctctcgg tgcgtcgcaa
     2521 cattcatcat gataattgca aaattattgc catcgacaac tccccggcga tgattgaacg
     2581 ctgccgtcgt catattaacg cctataaagc ccctacgcca gtagacgtca ttgaaggtga
     2641 tattcgcgat atcgccattg aaaacgcatc gatggtggtg ctgaatttta ccctgcaatt
     2701 cctggaacct tccgagcgcc aggcattact ggataaaatt tatcaaggac tgaacccggg
     2761 cggcgcgctg gtgctttcgg aaaaattcag tttcgaagat gccaaagttg gtgaactgtt
     2821 gttcaacatg caccacgact ttaaacgtgc caacggttac agcgaactgg agatcagcca
     2881 gaagcgcagc atgctggaaa acgtgatgct gaccgattcc gtggaaaccc ataaagcacg
     2941 cctgcataaa gccggttttg agcatagcga gctgtggttc cagtgcttta actttggttc
     3001 actggtggca ttaaaggcag aggacgctgc atgatcgact ttggtaactt ttattctctg
     3061 attgccaaaa atcatctttc acactggctc gaaacgctgc ccgcgcagat tgctaactgg
     3121 cagcgcgagc agcagcacgg gctgtttaag cagtggtcca acacggtgga atttctgcct
     3181 gaaattaaac cgtatcgtct ggatttattg catagcgtaa ccgccgaaag cgaagagcca
     3241 ctgagcaccg ggcaaattaa acgcattgaa acgcttatgc ggaacctgat gccatggcgc
     3301 aaagggccgt tctcactgta tggcgtcaac atcgataccg aatggcgttc cgactggaaa
     3361 tgggatcgtg ttctgcccca tctttctgat ttaaccggac gcaccattct ggatgtcggc
     3421 tgcggcagcg gttatcacat gtggcgcatg attggcgcag gcgcgcacct cgcggtgggg
     3481 atcgacccta cgcaactgtt tctgtgccag tttgaagcgg tgcgtaaact actgggtaac
     3541 gatcagcgcg cacatttgtt accgttaggt attgaacagc ttccggcact gaaagccttt
     3601 gataccgtct tttcgatggg cgtgctttac catcgccgtt cgccgctgga gcatctctgg
     3661 cagttgaaag atcaactggt gaatgagggt gaactggtgc tggaaacgct ggttattgat
     3721 ggcgatgaga acacggtgct ggtaccaggc gatcgttacg cgcaaatgcg taatgtctat
     3781 ttcatccctt ccgcgctggc gctgaaaaac tggctgaaga agtgtggttt tgtagatatc
     3841 cgcattgtgg atgtgtgcgt taccactact gaagagcagc gacgcactga atggatggtt
     3901 accgaatctc tgtccgattt cctcgaccca catgatccga gtaagacagt tgaaggttat
     3961 cctgcgccta aacgcgcggt gctgattgcg cgcaagccgt aaaggtctgg tattactgcc
     4021 ggatgcggcg tgaacgcctt atccggccta caaacccttg ctaattcaat ctattgcagg
     4081 gactatgtag gcctgataag catagcgcat caggcagttt tacgtttgca taacctcagc
     4141 gcccgtttcc gggcgctatt cacttcttac gcctgaacag caggcttatc aaatgccgtt
     4201 aacttcggtg cgttacccgt atatttttcg atatacacca gcgcagagtt accggcacag
     4261 gcatttgcca gctgcgagct ggggatatcc gccgttaaca cgttcgcact gccgttttta
     4321 cacaagccgt tttccagatc tggccatgca ccttcatgca cgcataccac gccttttttg
     4381 atcccgtcag tcaccaccgc gcctgtcaaa atctgaccac gtttgttcca gacacgcacc
     4441 agatcgccat tcgcaatacc aaatcgagca gcatcttcgg tgtgaatagt gatcggttca
     4501 cgatcggcaa ccgcatattt tttacgcagt tccgcatagt taagctgact gtgtaaacgg
     4561 tgtgccggat gtgcggtcag aagctgcaac tgcttctcgt cggcggtacc cttccactca
     4621 tcaggcgcaa gccaggttgg gtgtgccggg caatccttat agccaaattt ttccagcgtt
     4681 ttggagtaaa tctcaatttt gccgcttggc gtacccagcg catttttcac cggatcggcg
     4741 cggaaatcac cataacgaac gtactgttcg ttcttctcgc tgcggcgcat ttcgatcagt
     4801 ttattttgct gccagaaggc attaaacatt ggcatagtga cgcgttgcgc acgggcccct
     4861 ttctgagcgg catcatagaa aaatttcagc cacgccattt catctttacc ttcggtatag
     4921 atctcttttc cgccaggttt gagtaattcc gcaagatcgg caaacacgtc aaaatcgtta
     4981 cgcgcttcaa attgcggagc aacagcctgc ttcatcggca caatatgctg gttgctgtaa
     5041 tcaccggtca tcgtcaagtc attgcgctca aacgatgtgg tgatcggtaa tacgatatct
     5101 gcgtgtttgg ctgctgcggt ccagtagcat tcagaaacga cgatcatctc cggtttctgc
     5161 caggctttga tcagacgatt agtatcctgg tgatgggtaa agttaccgcc acccgcccac
     5221 cagatcattt tgatattcgg atacgtttgt tctttaccgt tatgctgata tttgccgcca
     5281 ggattttcca gcgcatcgac gatacgggca acaggaatcg ccgccattcc gccatcatca
     5341 gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt ccggcagcac gccgccaaca
     5401 cgcatcgggt tgccaccatt ggagtaatga taagagaaac caaaaccgcc gcccggtgta
     5461 ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc agtgtttctg ttcaccatat
     5521 tgctggcgct gaattcccca gcctgccatc agaattgtgc gattcgccgc catcagttca
     5581 gcgagtttga ctatctgcgc ttccggtacg ccagtaattt ccgccgccca gaccgcgctc
     5641 ttcggcgtat tatcgctctt acctgtcaga tactcttcaa actgcggata cccggtagtg
     5701 tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca tcagggtatg cgcaatccct
     5761 aacatcagtg ccacgtcggt gcccatattc ggcgcgatcc aggtggcatt atcgccaaag
     5821 aattcgatgg tttcggagcg gataggatca atggcaatca ctggtttgcc agattttttc
     5881 agctgatgga agtattccag cccttgctca tcggtactgc tccaggcaat ttttaaggta
     5941 ttcagcgggt tcattcccca cagcaccaca acctggctgt tttccagaat cagcggccag
     6001 gaggcctgct gttcatacac ctctacagaa ccgaccacat gcggcatgat cacctgtgct
     6061 gccccggttg aataatcgcc gctatgcccg gaataaccgc ccgccaggtt catataacgt
     6121 tgcagtaagg tttgcgcttt atgcaacacg ccagaagagc gccagccgta agatccggca
     6181 aaaatggccg atggtccgtt agctttacga atacgctcat gttgctcatg aatcagtttt
     6241 aatgcctgtt cccagctcac ctgtacatag gtatcttcgc cacgaccttt cgccggttgc
     6301 agtggattgt cgagatagct ttttctcacc atcggatgct gaatacgcgc cgtggtgtgt
     6361 acctgatccg ccgccgtaga ctgtaaggag ttcggtatgg ttttcgccag cgcgcctgtc
     6421 gaagaaacaa tcttgccgtc cttcacttct acgctcatcg ctccccaacg tcccgcggtg
     6481 aggattttac cgcccttctc ttctgcccat gcggggagcg gtgctgccga tgtcaccacc
     6541 agcgctccag cggcaatacc gctgtgttta ataaattcac gtcttgttaa tgccataact
     6601 tcctccctga tcaacgagga tcactgtttc tcggtaatat ctttggcgtt gtactggaaa
     6661 taccgcgtta aaatgtccag ttcgttttcg ctcatgctgg ttcgtgcccc cattcctttg
     6721 gcaatggacg gccacgcatt gacggtgtaa tggtcggcgg caatagaggc atgacaacca
     6781 gcgcaatagg tatcgtcaag tttttcagcg tattgccata gcggtttacg gtccgctaat
     6841 gcgggatcgg taagcgcacc ctgtaaagac gcctgacgcc attgattgcc gtattcgtca
     6901 gcctgccatt ccccgtttac cgtgagcgcc ttgatacctt cttcacttaa tgtggctagc
     6961 gccagccgtt gacctgccgc caggtagagc gtgttttcac tgccctgcat ttgataaccc
     7021 tgcaacagaa cgatcggctg tttgccactg gcatcaacga cggtgagatc ggtaccagga
     7081 ttcacggtag ccagctcgcc tatgcgagaa gttttgaaag gataaatatg tgcgccatta
     7141 gtcactgaag tagcggcctg actttccagc tcatgcgccg cgttgtcatc catttttatt
     7201 tctggcggaa aatgggcaat gcctttatga caatcgatac aggtttcgcc gcccttttgt
     7261 gctttgttat gcattttctg cgcagattca ctttgcgagg caatatccat ggcatcaaaa
     7321 gaatggcaac tacggcacgt tgcagagtca gttgctttta attctttcca tactgtttcg
     7381 gccatttcct ggcgatgagt ttcgaactta tcgtcactgt ctattttgcc gctaacaaat
     7441 tcatgataaa tatctttaga tgcttttaat ttagcaaata aataatccat ccctgacttt
     7501 ggaatatggc aatcggcaca ttccgcacgt atccctttct ggttcgaaaa gtggacagtt
     7561 ccctgatatt cctcaaaagg tttactcatc gagtggcagg aaaggcaaaa tgctgtatcc
     7621 gacgttttat gtaaggcttt ttgcgccagc aataacccgc cgccaccaac cacaaccgct
     7681 atcagcaaaa acaataaccc aatgcgtttt ttccctcgca tagattcctc taaataaatt
     7741 ccagaacacc gaaatcattg tataattaaa tacacaacga attaaccgct tgcataactc
     7801 ggcacaacac tgcctgaaat aatcgataaa gaatatgatt tattacaatg taatcattaa
     7861 ttgctaagga ataacccagt tgctatttag aatatatatt tatcagtttt agcaatttaa
     7921 atcccataat caatgtgaat ataaaaaata tttttagcac aacgctatgc gcactgtcac
     7981 ccgctcaaca gatcacggtt atcgttcgtt ttttatactg ctcagggata aaaaaaggcc
     8041 cctgttgaaa ttgcaggggc ctggtacgag caagcatcat attgggcgac atgatgcaac
     8101 ggtaaaaatc atttggcctg atggcgttca atgattcctt tcatttcagc aaccgccgcc
     8161 ccgtctacga tataacgcga atactcgtcc gcgtgttcat ctgatgacat ggacaatcct
     8221 tgattacgat aacgcatcgg tgaggcttgc cacgctcccg cggagctatg gacttccagc
     8281 actccggcat cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc agccataatg
     8341 attggagcat cacgatgggc aataagttcc ataatttttg ataaaccttg cagtgcgtct
     8401 gatttttgcc ctgatgtcag tacccgggca atgcccaatt ccgcaagatt attgagtgta
     8461 tataaagggt tagcgcacat atcgaaggcg cgatgaaaag tcactgccag cggaccggca
     8521 gcagccatta ttttttccat tcgtggcata tcgacattcc catcaacatc gagaacgccc
     8581 gtcaccagtc caggaaaacc taattcgcgg accgtgcgca catcctcaag aatggcggca
     8641 aactcaccgt cgctgtagca aaaatcacca ccgcgtgggc gaattatcgg atgcacaggg
     8701 atcgtcaccc gctggcgcac ggatttcagt ccccccaacg acggcgttaa gcccccctct
     8761 tttggggctg cgcataattc aactctgtct gcgccgtttt gctgcgccgt tagtgcacat
     8821 tccatgctgt aacagcaaat ttccagtaat gccatttttg ctccttaatt acgctgactg
     8881 ctcgctggca acgatctctt caatggacca cggatgaaac ttaatagttg ttttgccatc
     8941 ggtgaccgcc agcgtcggat tcggtaatcg ctcatgctca ccttttggtg aactggtttt
     9001 aactgagatc cccggtaggc tgagtccttc atcagaaagc aatgccagcg cacgggcatt
     9061 cagcgtttct ggatcacccg gcagaacgat ttcaatatgt tcccaacctt cgtgtgggta
     9121 acgtttttcc ccaggccacg gtagctccac attggaaaac tgccagtgcg caacctgtac
     9181 cggttcatgc aatttaaaca gacaaatcgg tctgccattg atcatttttt ctgacaaaag
     9241 ctcgccacac tgttcaaaac cgcgacgcca gcgctcagcg gtgacgtttt gatggcaacg
     9301 caaagaaatg tgatcggcag tcagcggagt gatatccaac cccagacggc gggaaagttc
     9361 atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct cgtcgataga
     9421 ttgccagttc gccataatca ctcttcgtct ttcagtaaaa gcgttaattt accctgttgc
     9481 cctgtgccaa ccaaccgctg atttcacgcc gcttctgatg caatagtgaa aacggcaata
     9541 cgccacgcgc acgttgctga cgaaaacagc catttgcagt atactcccgc cctaatttct
     9601 ttaactggtg cgggcaattt ttgctcgctt catcaatgta aggtattccg gtgaatattc
     9661 aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt
     9721 gcgaaccgca gattcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca
     9781 tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga
     9841 ctcatctgga tcttaacggt atcgccagca aagttgagat cgccggtcca ggctttatca
     9901 acattttcct tgatccggca ttcctggctg aacatgttca gcaggcgctg gcgtccgatc
     9961 gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt tgactactct gcgccaaacg
    10021 tggcgaaaga gatgcacgtc ggtcacctgc gctctaccat tattggtgac gcagcagtgc
    10081 gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca
    10141 ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa
    10201 tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gacgaagatg
    10261 aagagtttgc cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt gacgaatatt
    10321 tccgcgagat gtggcgcaaa ctggtcgaca tcaccatgac gcagaaccaa atcacttacg
    10381 atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg cgaaagcctc tacaacccaa
    10441 tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa agcgaagggg
    10501 cgaccgtcgt attccttgat gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca
    10561 ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc
    10621 gttatgaaac actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac
    10681 acctgatgca ggcatgggcg atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc
    10741 tggaacacca catgttcggc atgatgctgg gtaaagacgg caaaccgttc aaaacccgcg
    10801 cgggtggtac agtgaaactg gcagacctgc tggatgaagc cctggagcgt gcacgtcgtc
    10861 tggtggcaga aaagaacccg gatatgccag ctgacgagct ggaaaaactg gctaacgcgg
    10921 ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct
    10981 tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgctt
    11041 acacgcgtgt attgtccgtg ttccgtaaag cagaaattaa cgaagagcaa ctggctgcag
    11101 ctccggtaat catccgtgaa gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg
    11161 aagaaaccct caccgtggtt gcccgtgaag gcacgccgca tgtaatgtgt gcttacctgt
    11221 acgatctggc cggtctgttc tctggcttct acgagcactg cccgatcctc agcgcagaaa
    11281 acgaagaagt gcgtaacagc cgtctgaaac tggcacaact gacagcgaag acgctgaagc
    11341 tgggtctgga tacgctgggt attgagactg tagagcgtat gtaatcgatt tttcgtgaga
    11401 gtgaagcctg atcagggtta gccgatcagg cttttttatt gccatctaaa tgtattctga
    11461 aaacggacat gccattgttt tctcactgtt gagtaagagg ccagaagcgt aatatccagg
    11521 cccaggaaaa cgataactgt tcaatttaag gaataccgaa gtgtttaaat ttcttgtatt
    11581 aacattaggc attatctctt gccaggctta cgcagaagat acggttatag taaacgacca
    11641 tgacacttca gccatcaaag attgttggca aaaaaattca gatgatgata ctgacattaa
    11701 cgtgatcaaa tcatgcctgc gacaagaata caatctcgtc gatacgcaat taaataaagc
    11761 ctatggtgaa gcttatcgtt atatagaaca agtgccacgc acaggtgcaa aaaaacttga
    11821 tactgaacaa cttaacttgc ttaaaaaa
//
LOCUS       DAJVQH010000121        11550 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJVQH010000121 DAJVQH010000000
VERSION     DAJVQH010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.121"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(1..337)
                     /locus_tag="OQM87_003344"
                     /pseudo
     CDS             complement(1..337)
                     /locus_tag="OQM87_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
     gene            complement(653..1297)
                     /locus_tag="OQM87_003345"
     CDS             complement(653..1297)
                     /locus_tag="OQM87_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509364.1"
                     /translation="MMKQGMANSISLTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1318..1587)
                     /locus_tag="OQM87_003346"
     CDS             complement(1318..1587)
                     /locus_tag="OQM87_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCS1509365.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPEKLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1666..2304)
                     /locus_tag="OQM87_003347"
     CDS             complement(1666..2304)
                     /locus_tag="OQM87_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCS1509366.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2289..3521)
                     /locus_tag="OQM87_003348"
     CDS             complement(2289..3521)
                     /locus_tag="OQM87_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCS1509367.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHILEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            complement(4022..6703)
                     /locus_tag="OQM87_003349"
     CDS             complement(4022..6703)
                     /locus_tag="OQM87_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCS1509368.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVKRAFSSQISVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVMARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RFSSQKTTDLMAYIHYLSDRRIGFRNRIDLLLNFMILKVKPLMIPERRLALFTSLQLS
                     YYEKNIREKQISLNEYEEVFKKSDFKILLGRLTSWSMLYLKQHLRRNVSTRSSFSAET
                     YRDEFDRFIKRFPIIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(6944..7990)
                     /locus_tag="OQM87_003350"
     CDS             complement(6944..7990)
                     /locus_tag="OQM87_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HCS1509369.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSAGTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            complement(8094..8243)
                     /locus_tag="OQM87_003351"
     CDS             complement(8094..8243)
                     /locus_tag="OQM87_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509370.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
     gene            8665..8856
                     /locus_tag="OQM87_003352"
     CDS             8665..8856
                     /locus_tag="OQM87_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1509371.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMRDGYPHPCM
                     QPMYNFLGKFKPEPREETE"
     gene            complement(8909..9142)
                     /locus_tag="OQM87_003353"
     CDS             complement(8909..9142)
                     /locus_tag="OQM87_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509372.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(9238..9861)
                     /locus_tag="OQM87_003354"
     CDS             complement(9238..9861)
                     /locus_tag="OQM87_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1509373.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(9950..10459)
                     /locus_tag="OQM87_003355"
     CDS             complement(9950..10459)
                     /locus_tag="OQM87_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509374.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            10917..11375
                     /locus_tag="OQM87_003356"
     CDS             10917..11375
                     /locus_tag="OQM87_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509375.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
BASE COUNT         3231 a         2874 c         2311 g         3134 t
ORIGIN      
        1 ctgattctgg aaaaggactt tgtggattgt tgctattgcc agtgagattg aatgaagtcg
       61 ttttaactcg ctttgaatac gtcttgcccc cagatttcgc tgagaacgta actccagaat
      121 cagagcgact tcgcaagtgc cggttttcgt tgatggtgaa cgcttggggc gacggctgtg
      181 gctttccaga ccagcgatgc cctgagccag atatctgcgc caccatttta cgcaatgtgg
      241 gccgagaaat gccacagcga cggcatacaa atcccgcatc accggacgtt tcatagagtt
      301 ttacccactg tagccgctgc tgaatttccc tgttcataat ttggcattag ttcagctcaa
      361 tcccgactta aaaagtccct acaggatgtt ccggacctga ggcacagttc atatccttat
      421 ccataaaaaa tttagctatg tctatagcat taatcttgac aagacgacgt tagggcctgg
      481 cgatgaatca tttcccctgc cagatatcct ttggggtgca ccagaactac cgttttatat
      541 ctatataact gaaacgtccc tcgccatcct tcatattttg atgtagtaac accattcact
      601 ttcatctacc ccccgtttta ttaacatata gtaaaaatca cctcgccgca ttttattctt
      661 ttgaagtaaa atcatttacc agttcactta aagctctctt ttgttcatca tcatacagag
      721 attgaatccg gaatttaatc tcttctgcaa ccagtcttgc atcgggctca taaaatattt
      781 ttgttattcg ctgtttctga tgttcaggct ggtcttccat atttcgtaaa accagatgaa
      841 ataatatatc ataaagtgcg caatgccatg aataaccatc attggttaaa ataagctccc
      901 aaacccgctc ctgctcggca ataagctgca gaacaaagga ctcaacctgt tctgtttgtt
      961 cgggggcaac acggtatagc ggagcaatca cagcaaaaga tagttcattt aaccaggatt
     1021 ccgcttcctt ctcgctcctg aacggtggac agttttgtat tcttcgttcg tattcacggg
     1081 taatctgctc cagaatatcc tccggcggtg tcagccactt attcctgagc aatacactgg
     1141 ttgatggatg acagctggct atttcagcaa taatttcacg taatgtttcc gggctgaact
     1201 tcttcagctg attgtagtgc tcccgggtaa aaccatgttc atcgcataaa agttgccatg
     1261 tcagtgatat actgtttgcc attccttgct tcatcattaa tacctgttac ctgaatatca
     1321 acagaccggt gtaccttttc tgaatacctc cccggacgga gtggcaatcc ccattcgcct
     1381 cagcgtggca tctgcagctt tcgccacctc gctgatatcc agcttttcag gtagcggcgg
     1441 ggacgatatc tgcgccagac aataattccg gcgcaacaac aaccactccc gttgtgcttc
     1501 tgtacattca acatttccca taacaaaaag ccagcggtca gaagctctgc gccagcaacc
     1561 agcagcctcc agtttctgcg caaccatgtc ttccacaact tccggggtac tctttttcct
     1621 gctcaaaaca ttaactcact gaaataatat acatatctga cggtctcagc gaaccgtcca
     1681 tgccgggctg aaatcctctt cctgaaacag ctccgtcagc ccgctaatct gtttcaatct
     1741 gagaacttca tcctggtcca tacccagctc cgtaccaatg cgttcattcg tccagcccag
     1801 gcgcgacagg tccctgacaa tatcagacat tgaggttatc tgatgtttgc ccctggcccg
     1861 gttatgtcgg atggtcgcag caatacgtga ggcctgtccc tttctctccg gattaataca
     1921 gaccaccggc agccagccct tcagacgttt tcccgtacct gcttccctgc ccagcacctg
     1981 gcgatgaaaa ccatccacca ccaggtaatg gttcttttcc tcagacacca ccaccggttg
     2041 tgtgaaacca tctttctcca gtgactgctt cagcagtttc ttctcacccg gcgccatgac
     2101 attaggattg taatcattgg ccaccacctc atctgctttt acccacagca cacagtccac
     2161 tggctcatgt gcaaaaggac tgacttcatg aagcactctg cgcaggtgat taagggcctg
     2221 tatggtctct tcttctggtc gttcctggct gaagtactga cgaatcatgt tgccaattat
     2281 ctccggtgtc acagaatccc ccattccttc ctcctttctt tcatacgctg cagataacgt
     2341 tcatagtgcc ggggtttgtt cggactgaag gagagggtcc ggcaccagaa atcattcttt
     2401 ataagtgtct tacagatacg ccgccaggac ggaatatccc ggctccccag gtcattctcc
     2461 tgttcatccg ggatgtcatc cgggaagccc cgcgtctgat accagcgcag gtagacagca
     2521 attttattac ggtaatgttc tgccgttctt tccggcatca catccagcag gaacatcgca
     2581 tagctgcgcc aggtatgatg ggctggtttt gatatacgtt tacgcagggc aaaataggca
     2641 ccgctttcat tggcataaag cgccccgctg gcagcgcctg acacccgctc acacatcctg
     2701 gcccaggttt ccggctccag aatatggtaa agccacagcc ccttacgttg ttcaggacca
     2761 aaaggttcac agacacgcat gttgcgtaac ggcacgtcgg cacggtacat caggtcatac
     2821 agaggattgt aaatggcaca agctctggcg ttatatatcc agatatcgcg ggctttccag
     2881 tcatacaacg gatacatggt gtaataaaac ccttcaggcg atgccgtggt ccagggctta
     2941 tcatcggcat atctcagttt gcgctgagac accagcccca taaagcgatt gagggattca
     3001 tccgcacgaa caccggtcag cactgccact ccacaccggt taccggcaaa ccaggaagaa
     3061 aatgccggaa caaattcttc aaacgtcatg gcataccgga aaaagggaaa atatgtcata
     3121 tccgtaatgg cctcttccgg tggctggcga acccaggtca ctcctggctc ccagcatatc
     3181 cactccggct gaaactgcga gacgccgttt accgtggtca ggggaagtgc cacccagtaa
     3241 aaggtttccg tcacatcatg gtacatttcc cgcatcttct gaatatgttc aatggtgcac
     3301 cgatactggg cctcccagtc aatgaacaga acagagaaat gacgttttct cctgcgggcc
     3361 acctctgcca caagatggaa aagcacggtg gaatcctttc cacctgaaaa agacagacat
     3421 accgacggaa atgtttcaaa aatccactca atgcgggcga ttgcggcacg caaaacattt
     3481 tctccggtaa ggattttttc ggttaaaagc gtatcactca ttttcctggc tttatgtcga
     3541 ataattctgt ttatacattc aggcaaactt cattaataac tcaccgcttt ggggataaat
     3601 aataacaaca aaaatcttca gatattaacc tggttgttgg atttgttatc acatccttta
     3661 tccacctgct tcttactggt aacacatctg gaaaatccat gcattttcaa catatactat
     3721 cctttcaact tgtaaataag aaaatatgat gcagatggtg aacaattcaa atatcatttt
     3781 gcaggaagta ttcactgtaa tagaattacc ccctgataaa gtttattcac taaggaatta
     3841 ttcaacagaa acattccctg ttagctttga gcaatagcac ttatttttat atgtatcagg
     3901 aatacgtacg ttgatgtaac caaaacaaag aagcctgtgt ccatggggtt cgtacaattg
     3961 tgtgtggcac atgaccaaat caggtttgag acctcccgct gcaatatttt ttattcagat
     4021 tttagcctgt caggcctgac agaaacgctc ccaacttccc ttctatatca ctgtctatag
     4081 tacgtaatct tagcaatggc aggccgcatt tcttcagtat actgttttta agttcatcac
     4141 gttcagcctg aacactggtc agatgataac ctccatccac ttcaataacc cctaatggag
     4201 tcttcccgac cttatagtaa acaacaaagt cacaactggc ccggttacgc atgaataact
     4261 gttcacggtg tgtaaaatcc ccccgctcca gcaacaccaa ctgattcagc gctatttgtg
     4321 aatgaaacat catactccga taactatcct gcgacaggat atcccgcaac aggcaggcca
     4381 caatctgttc tgacttaaag tgtgaatcat ttgaattcaa tctactattc agtcgttcca
     4441 gtgatttgtc atactcactg tacagcaaat caaatgctga tatgaccggg ctctcgaaaa
     4501 tctctccgtc atcagcgtag tatttgatgt aacgaatcag cgccgcaata tgtccagcgt
     4561 ggcgttcaaa tacatcatta ccagtcacca gcgtaaattt atttcgggcc cttgaaacag
     4621 cgacattcac cagttcagga ttatcaacaa acgcgatgtt ccgactgtgc tggctgctgc
     4681 gcttcttatc cagcacagtg gaaaacacaa tttcatcgca ctcccgtccc tgaaatttat
     4741 gcaccgtcga tttcacaaaa tcagcaggca gcactttttc agccagatta acctgtgcat
     4801 tatatggggc aatgtatccc ctgctacttt cttcatccca gtaatgacct tccagagatt
     4861 caagctccct gagatttgaa aaattacggg tatggtttcc ttttgccgtg atgaccagag
     4921 ataatgaggc ttcaccggaa tcaaccgtta acggtatcag agcattatca taaaactgct
     4981 tattacaaaa ttgaatgatt ttaggatgac aacgataatg ctctttaaga agggttactg
     5041 gcaccatgtt cctgaacaac atacaaaccg aatcaagtag actatatttc tcacaattat
     5101 agtattctgc cggtggagat gggctgttcg ggagtaatac aggaacatgt ggaagttgct
     5161 tacgatcccc tacaacgatt acattacggg cacatcccag tcccagaatg cccggaacaa
     5221 tatcctgctg cgaggcttca tcaataataa cataatcaag aagggccccc ttgccgatag
     5281 agttaataat cgaatgcgta ctgctaccaa taatcggaaa acgctttata aaacggtcaa
     5341 attcatccct gtatgtctct gccgagaatg aacttcttgt tgaaacattt cttcgcagat
     5401 gttgctttaa ataaagcata gaccatgatg tcaggcgccc cagtaaaatt ttaaaatcag
     5461 attttttaaa tacttcctca tattcattca gagaaatttg tttttcccgg atattttttt
     5521 cataataaga aagctgaaga gaagtaaata gcgccagcct tctctccgga atcatcaatg
     5581 gtttgacttt cagtatcatg aagtttaaca acaaatcaat tctgtttctg aatccaatcc
     5641 gcctgtcaga aaggtaatga atataagcca tcaggtcggt tgttttttgt gaggaaaatc
     5701 ggtatttgtg agaaaaaagc tcctccggtc gaagattctg actctgccat tgctgcaggt
     5761 atttatactc aatattcagc tcattaattt ctgcttttaa cgaagcgacc tggttaatgg
     5821 cattcaggtg ttttttcact tgttgaagta catcatcaat cgcatttgca gacggtgagt
     5881 caggtaagac ttcctcagaa cgcgaaatcg acgttgaaaa aaactgttgc ctgttctccg
     5941 tactccccag tcgtgccatc acatatccca gttgctgttt gtccatcttt tcataaacat
     6001 tactgacagc tgagttatta ttggacaaaa ttgccactgt tttattctga attaatatat
     6061 tagcaacaat attgagtata gtctgtgttt tacctgttcc aggtggtcct tcaataactg
     6121 aaatctgtga ggaaaatgcc cgtttgacag ccagcaactg actttcatta agaccaaacg
     6181 gaaaaatcaa accccatggc gaatcacgtt ttttactctg tcctgaacag tatgcatgca
     6241 aggcggtatc cggatgagga actattttct ttatctgcct aaggatattc tcatcgatat
     6301 ttctgttatt accacgggca taaaaaatac gttcattagc caccctacac aagtaattaa
     6361 acacggcatg ctctgtaaat gctgaacact gttgaaagtc aaggccggtc gttttcatga
     6421 tgtagggtcg ttcaccctcg cggtaataaa cagcagcata tttatcacca tatataataa
     6481 ccagttcagc ccgggatgtt acagcgttcc ctttcctttc gaggaaaaca tttcgcaaat
     6541 ttacagttgg cgttatctga catacagaaa gcggacgagt atattttctc ccgctgcgaa
     6601 aatgacaggt cagcataacc tctctgtcgt catttccagc ccacagcgtc cagtcggtaa
     6661 ttttatgagt cttattttca ccgtcaacac aaatcgtgac catattctca ctcagaaatt
     6721 atcatttaac ttacggaaaa ttgttaaggc actcccgata ttagctccgg aggattttcc
     6781 acaactccgg gactgttaac atatttctgc ctgccatata tacattgaat ttagtcataa
     6841 cagtcaatac cagaattaca ggaatgcgat gccgatacgc tggttattga atactggtag
     6901 cccctgagca ctaattcatc acgatactct gtatttactg ttttcactgc tttttacaac
     6961 cattccccca ggtaaaatgc tgctcctccg gtgatggctg tgtcagaaac tgccatcccg
     7021 gaatagccac gctgaccgcc agcaatgccg cacagacacc agccacaaac ggtttcagaa
     7081 acgtgggcgg ggatttctga agggagggtg actgctccgc ctgcactttc cccttgcgat
     7141 tatcaaggct cagtgcatcc agacgcaccg cgccatttgc cgcaagcagc ccttcccggt
     7201 tatccacgct tcccggcaga ctcagcgaaa aatcccctgt tcccgtctga atcagactgc
     7261 ctttacgatt tgacaggctt tgtgccgata tctgcagtgt gtcggcattt atcgtaccgc
     7321 cattactgct gaactgcccg gcagtccggg cggaaagcgt accggcactc agttgcgcat
     7381 tctgtgtaga cagatttcca ctggttgcat tcagctcagt atgttgtccc tgtatccggc
     7441 tgttacctaa atccagcccc tgctcaatgt cccgtaaatc cagcagagaa ccttcgtgtg
     7501 ccgggaatga ggaatacaac tgaatatagt catcactgtc cggtgtcaga cgcacatgtc
     7561 gcacaacacc gggaataatg accagccgga ggataccgct ttttaaatcc tgcgaaggtg
     7621 ccagaacacg ggtggtgaca taaccatgat cgaccagacg gttctgcaat gtactcatca
     7681 gcagattaat tcctttcgcc cccaggcaat gcccgaccgc cccgttggcg attttttgta
     7741 aaggcagcca gtgtggtaac gcatcagccc ccttcagttc cgtctgttta atctgaaaac
     7801 agggcgtttc aacaggaaaa ttaatcttat gggcaaaaga gccaggtgca gaaaggcgaa
     7861 catcaggggc tgaaggcgtc agttgttgct caagggcttc ctgacgctgc tgctgaatga
     7921 taaatgcttc atctccgcta cgccggacat ccgctgcctg aagagataaa ggcgataata
     7981 aagcagacat cagtgtcagt gacagcaagg acggacagcg ccggaataag ctttgttcct
     8041 cagtcctctc gttcctgcaa cataattttc cagtcaaacg aaaaaccttt tattcatgtc
     8101 ataccaacaa tcaccagcct cctgacgata cctcccacca tgttttgcga tggcacgcta
     8161 ccgcccttcc ggtatttacc cgcatcaacc ggtaccagaa tgctgagtaa caaaccagcc
     8221 agaattgact taattttgat cattatagaa taatgatctg aactacagca acatgacaga
     8281 tttatcgatc gatataacac aaatgaacag ttctttattc tgccatatat cgcaaaaaca
     8341 gcacattaca aaatttataa ccataattac tggaaaagtg cagaaaatta ttccactgaa
     8401 tgcttttaat acagttggtt agcttaaaaa atagcaattt ttgatttcag tcattgaaag
     8461 ttgcaatgtt tgacgattca acagtattta agtgagtttc aggatatggg aaggacatga
     8521 aaggaggagc gcaataaaat acacaggaag aaggggaaaa tgtttcgtaa aaacagaaaa
     8581 ttaacagcgc acatcaggaa acgcagtctc ctgattcagt gctaccacat gaaaatttag
     8641 ttaactgtta ccgtactaaa atttatgtca gaagatgttc cattaccgaa agtcaatcag
     8701 cgatacaaag acgatcatgg tgcgcttgtc accctgacca gtgttgagga aacccgggtg
     8761 gtgtttatgc gggacggcta tccgcatccc tgcatgcaac cgatgtacaa ctttctggga
     8821 aaattcaaac ccgaaccccg ggaagaaaca gaataaaacg aaatccctgc cggcaatcac
     8881 actacctgat agcagcataa tccacttatt aattctcatc cccggagaca agcccagaaa
     8941 accactccgg gtgttcactg tatgccgtgt tcagcgcatc cctggaaacg tttatataaa
     9001 agctgtcctg caatttgcgg gtaacaactt ctgccaggcg attccggtta tccaccagtc
     9061 cggcataacc tctctctgcc agccactcat tcagttcatg ttccagagac agattgataa
     9121 tatcataatc ggaccttgcc attcccgttt ccttagcaca tgttttcagg cacggggata
     9181 aagtataaag aatgtcatac tgctgctgcc agagcaatat tccccctgat ggttgtatca
     9241 gagatggtat gccggtactt tgcgggttgt tcccgtcagg ttatcgtgca cctccacggt
     9301 cgtggtcacc accggcattc cggcctttct cagcctcaga acatcagccg caatacgctg
     9361 actgccaaac cagaacaggc cgtccagtgc agtcaccagc aaacccgcct ccagtgcata
     9421 cttcagccgt tcacggggcg ctttcacttc ccgggcaacc tgctgatatg gcaatgatgt
     9481 gttttcattc ccaaccacgc gacgaatacg gtgagacaga tgataccggt atgtatccgg
     9541 cacaccagaa aggctggcct tcaggctgta cacgcagcca aaccgtttat cgttaaacac
     9601 cacatttttc tggctgatgc cccattcttc acgcagcgcg gcaatcaatt gcggtgtacg
     9661 ggtaagcagc aagcgaaaag gcagttcaaa actggtgacg taatccacat tcagcagtgc
     9721 aatacgcaac cgttcttctg gcccggcctc tgtctgccgg cactcctcca gaacatcctg
     9781 ccactgccgg cgaagacggg gggattcgtt ccgttctgta aaacagtatt tatccgccag
     9841 atagtcaatt cgtgtacgca tactgaaagg aattccgtat aaagattcag cagacaaaac
     9901 tttatcagcc agtaaaaatt aacggaagca ttgatattcc tcccgacaat caccggatga
     9961 ttgttgcaat aacctgcgac ataaaggact gaacagccgt ttttaacgcc acgtattgcc
    10021 ctgatgaacc aggccgggca acccgaaaac atctttccac ccggcattgt ccgccagcac
    10081 ttatcaccga ccaggtctgt tgcagtaaat ccaccagttg cgcacattct tcatcagcca
    10141 tgacgccgac tcgcggatgg caaatactcg tctggctgag cagcaacaga catcgtatct
    10201 gtgctccttc atgttgttcc cgcaaccagc gtaataccac cggcgctgat gggctgaccg
    10261 tgtgattatg ctccgtaata cgttcgtgta cccgtcggtg aaaggaacta caaaatgtct
    10321 gaatctgttg accatagatt ccttctgccg aatagagcgt gaagtgaaat gccaacagat
    10381 ttggccagtg gtccaccgcc tgctgaacaa aacgccggat ttcccccggc tctgaaatta
    10441 aggcttcggt tatttgcacg aaatgttcct tttcaacccc tgttgaattt tgtcaagccg
    10501 gtgcagacac agtaaagcgc gcatcaacac ctgaaaggta cgatacaaac acaagttaca
    10561 aacaataaga acggaaggac aattaatcag tgaggaataa taactaaatt aaccaaaaga
    10621 atgttactga caatactacc aatacttaaa tagcaatata ttaattaatg taagaacaga
    10681 tgcccggtaa gacatataca caatacatcc acaaacacac tcttttcatt aaatcaatca
    10741 cctgattaaa ccatgaaagt gtgatgtaag tcaaaattac tgatactatc aattgataat
    10801 gattatctct atcattatcg tgaaattcga gaatgaaagg agaagtagtc attataagcc
    10861 ttttataaat cgtcatcatc atagtaaagt agaacacttt ttatatgagt tactatatga
    10921 aaatatactg gacaagaaaa cgtattcctg aactttcagc acttccacct tcattaagaa
    10981 agaaaaattt tactgacgca tataatgcag catcttctca tatcgaatac tggataggtg
    11041 ctggcgtctc atttatctcg atgatgattt tatccagagt atatgacttt cttttacccg
    11101 cccaagatac tttcccgggt gatatcatca gaagtttatg tgttgtgtgt ccatccatat
    11161 taatctggtt tcagttttcg gtatatgtca tgaggaaata ttacagacat attctggtgc
    11221 gaggaaaaga aaccgaaacc atttcagaaa ggttgatacg tgaggctgat acacgagaat
    11281 atgaactatg gcgtccggtc cgtcgctttt tctccattgt tttcctcatt gttctattag
    11341 gttgcattca ttcactgata acaactataa aataaaagtt atggctgttc tgatacagga
    11401 aaataaacgt tcattctatg gttacctctt cctgttatat agaagaattt taaacgacaa
    11461 gtaagggaat ataaatgaaa ataacaaaaa ataaaaatgc aactataaaa aacagtcatc
    11521 agtcggctgc ttttcaatgt ttaccttatc
//
LOCUS       DAJVQH010000122        11457 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJVQH010000122 DAJVQH010000000
VERSION     DAJVQH010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.122"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..447)
                     /locus_tag="OQM87_003357"
     CDS             complement(<1..447)
                     /locus_tag="OQM87_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCS1509376.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPI"
     gene            576..911
                     /locus_tag="OQM87_003358"
     CDS             576..911
                     /locus_tag="OQM87_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCS1509377.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPIFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(908..1630)
                     /gene="folM"
                     /locus_tag="OQM87_003359"
     CDS             complement(908..1630)
                     /gene="folM"
                     /locus_tag="OQM87_003359"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCS1509378.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(1667..3049)
                     /locus_tag="OQM87_003360"
     CDS             complement(1667..3049)
                     /locus_tag="OQM87_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1509379.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(3235..4179)
                     /locus_tag="OQM87_003361"
     CDS             complement(3235..4179)
                     /locus_tag="OQM87_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1509380.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            4703..6235
                     /gene="pntA"
                     /locus_tag="OQM87_003362"
     CDS             4703..6235
                     /gene="pntA"
                     /locus_tag="OQM87_003362"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCS1509381.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            6246..7634
                     /gene="pntB"
                     /locus_tag="OQM87_003363"
     CDS             6246..7634
                     /gene="pntB"
                     /locus_tag="OQM87_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCS1509382.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(7659..8693)
                     /gene="tqsA"
                     /locus_tag="OQM87_003364"
     CDS             complement(7659..8693)
                     /gene="tqsA"
                     /locus_tag="OQM87_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCS1509383.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQLLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            9104..9469
                     /gene="mdtJ"
                     /locus_tag="OQM87_003365"
     CDS             9104..9469
                     /gene="mdtJ"
                     /locus_tag="OQM87_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCS1509384.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            9456..9785
                     /gene="mdtI"
                     /locus_tag="OQM87_003366"
     CDS             9456..9785
                     /gene="mdtI"
                     /locus_tag="OQM87_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCS1509385.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(9824..10645)
                     /locus_tag="OQM87_003367"
     CDS             complement(9824..10645)
                     /locus_tag="OQM87_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCS1509386.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(10745..10828)
                     /locus_tag="OQM87_003368"
     CDS             complement(10745..10828)
                     /locus_tag="OQM87_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509387.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(10921..11256)
                     /locus_tag="OQM87_003369"
     CDS             complement(10921..11256)
                     /locus_tag="OQM87_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCS1509388.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
BASE COUNT         2863 a         2758 c         2942 g         2894 t
ORIGIN      
        1 gatggggtcg atagttaacg tgccgaaatg cagggctttg tagggagtca gagacgtttc
       61 ctgaagacct ttggtcagtg tggcttgctc attctgacgc aaatgcaaac gtaaacgcgc
      121 tagcaaaaca gcagggggcg tcgttttgag aatatagtcg caggcaccca tttccagtgc
      181 caggatgtgg ttcatatcgc tatcgagaga ggttagaaga acaatcggtc cagaccactt
      241 tgcgcgtaaa tcacgacaaa tggtcatgcc gtccttgcct ggtagcatga tgtcgagtaa
      301 caccaaatcc ggattttctc gcaaaatggt ttcttcggcc tggtcgccgc gcggttctac
      361 ggtaacctgc atatcatgtt ttgccaggta cgcggcaatc agtgaaccga cttccgcatc
      421 atcttccaca aatacgatag tgttcataac attcacggta gatataaaaa cgtcacaata
      481 caccgcgctg tttttactta ctatcaatcg tttctaaaca agttttcatc aggtattcta
      541 ctgatgactt gttatggaag tgttaaggta aaaagatggg gctggtaatc aaagccgcgc
      601 tcggtgcgct ggtagtgctg ttgattggtg ttttagcaaa aacgaaaaat tattatatcg
      661 ccgggctgat tccacttttc ccgatctttg cgcttatcgc gcattatatt gttgccagcg
      721 aacgcggcat tgaagcctta cgcgcaacca tcatttttag tatgtggtcg attattccct
      781 attttgttta cctggtgtcg ctgtggtatt tcaccggaat gatgcgcttg cccgccgctt
      841 ttgttggttc tgttgcgtgt tgggggataa gcgcatgggt gttgattata tgctggataa
      901 agctgcatta acgcagatga cgaccgccat caagtgggaa actgcgtccg gtgacaaagc
      961 aactggtaag taagtaatcg accaggtcga tcacttcttt ctcgccaggc gcggttttca
     1021 tcagtgattt attcagcgcc tgttgtcgat attcggcatc atcatgttca ttaaacagga
     1081 tcagcgatgg cgcaatagaa ttcactttca cttccggtgc cagcttgcgg gcaaacgagc
     1141 gggtcatatt atccagtgcc gctttgcttg cagcatacgc aacatgtttg tcgctgccgc
     1201 gctccaccac ataatcggta aagtgaatga tatcgctggc ggcgtgtccg tgcccacgca
     1261 gtaatctttc cagcgcatgg ttgagcaggt atggggtatt aacgtggatc tgcatcatgc
     1321 aagccagtac gtcggccagt ggcgcacccg gtttttccgc aatccacgca ctggcgttat
     1381 gcaaaatagc acgcagacca tgggtggttt ttagtacttc atcggcaaac gccatcacac
     1441 cgtcgttggt cgaaaaatca gcctgaatac actgcgcacc tgcattaatc agtccatcaa
     1501 tggctggata gtgtgtccga tagctgacaa tcaccggttg cttttgatta atgaaatgcc
     1561 atgcgagggc gaggccgatg cgacgacctc cgccagtaat taatattggc aagggctggg
     1621 ttttacccat cgttatctcc tttgctatcc aacgatggga tgaacgttat cccaccagca
     1681 tccaggtcgc cggaactgag gcaattaaca gcatgccaat cagcaccatt tcctgacggt
     1741 tcagtacatt atcatgtgta tgcgttttgc gcgcatacag gaaaaccagc agccccggtg
     1801 catacagaac aacggataac agcaagtgca ttggaccaga ggcgtacaat aaccataagc
     1861 cataaatgca ggcaccgaca cctaccgctt tatgcagtgg acgggttgcg attttcagca
     1921 agaacgcgcc gaccaggaaa tagggcacca gaatcatttc tgaggcgatg gtcagcaacg
     1981 tgttgtaatc ggaaccggtc agccagatga gtaccagaca aatttgcacg cagatgttgg
     2041 tcaaccacag tgaggcggat ggcgcagctt gtgcgtcctg gcgcgcaaaa atgcgtggga
     2101 atgctttatg agtggcagcc aggaacggaa cttctgccgc catgatggtc cagcttaagt
     2161 acgcgccgca aacggaaacg atcaaaccgg cagcgatgat gatttcgccc catggtccca
     2221 tcatttccac catcagaccg gccattgacg ggttacgaat ttcagccagt tcaggacgtg
     2281 ccaccacacc cagcgaaagc agcgttacca gcaagtaaac gcccagagcg gagagaaccg
     2341 ccagcagtgt cgctttgcca acatcacgtt tattacgcgc acgcgcagaa acaaccacgg
     2401 caccttccac accaatgaaa acccacaggg tgatcagcat ggtgtttttc acctgttccc
     2461 aaacaggtac gccaagtgca agtccggtga agtcgagctt gaaggtatcc agtttgaaca
     2521 tcatcatcgc cagcacaaca aacagaccca gcggcaacaa ttttgccagt gtcgccacca
     2581 ggttaatgct ggcagcggtt tgcaccccgc gcaggatcaa aaagtgaaca atccacaata
     2641 aagccgatgc accgactatc gattgccagg tattaccatc gccaaacagg cgcaattccg
     2701 gcgtgtccgt aaaaaagctt aacgcggaaa aaacgatcac cagataggag acgttggcga
     2761 tgaccgcgca cagccagtat ccccatgcgg aacaaaagcc gattagctcg ccaaaccctt
     2821 cgcgggcata ggtaaagata ccgccatcaa gttcgggacg aatgcgcgtg aggatcagca
     2881 tggcaaaggc cagcaataaa atgccagcgc cagtaatacc ccagccgatg agcagtgctg
     2941 ccgggctggc aactgccgcc atattttgcg gcagactgaa aacacccgcg cccagcattg
     3001 agcttaatac cagcgcggtg agtgcgctca gtcccagttt cttttccatc gatatccagt
     3061 gattaaaacg tgataagaag aataaatatt agggcaaaac gcataaaaat ggagtttgcc
     3121 actgaagcgc gggattttac gaaaagatgt gtctgcatgc aatggtcgcg gggaaatttg
     3181 tgactaaaaa tgcgaaaggc ggcatcaagc cgccttatag gagtaactga atagttattt
     3241 atacagatct gcgctgacgg tcaggttatt accacgtttc tgccactggc gggtgatgtg
     3301 gtaatactta gcacctttct tcgcggcacg tttcgcaacc tgataggaga cttcggtcat
     3361 gttgccgtag ttgccagaga atttgatgct gtcgaacggg accatcatcg ctgctgtcgc
     3421 tttgttcagt tcttcgactt tagtgccatc cgggagcgtg acggtgtaac gcccgccttt
     3481 tgatgactgg gtttcaaaga agcgaccgac ttcagaactt ggtgatgcgg tagtcgcaac
     3541 acccgggatc tcaactttct tcgcggcttc gccaccggca gccagagctg cacgtcctgc
     3601 ttcggaatct gccgggatca catccgggct ctggacgata cgtttcttag catctttttt
     3661 atagatgaat gcagtaatac gctggttgcc gccctggttg gcatcgattt gacgaacgat
     3721 gtagaaagag taggcacctt tcgcttttgc cgctttggtg atggcatcat tgacttctgg
     3781 ctggctacga tagaagccct ggacggtgac cgtgtcaaac ggttcaatca gaacagcctg
     3841 atctttcggc agttcgacaa caccgttaat tacgcgatta cttgtttctt ctgctttttc
     3901 agcatcggct ttatagaggt cagcgaccac acgccagtta ccgctgttac caaaatcaga
     3961 agtgtcgaca acataaaaag aggcggcacc ttctttatct gcgcgacgag aaacggcttt
     4021 caccgcttcg ccaatagcat taaaacgacc ggtaaccact acacggtcaa aaggtttaac
     4081 cgctgccgct tgctccggtg tcagttctgt tgctgcgtta acggagaatg ccgtagcaga
     4141 aagcagtgcc gacgccagga gggtgttctt aagcttcata aaaataatcc ttcgccttgc
     4201 gcaaaccagg tactggtatt gttattaacg agaaacgtgg ctgattattg catttaaacg
     4261 gtgtaactgt ctgcgtcatt tttcatatca cattccttaa accaatttta atcctgctca
     4321 aatgaccgtc tatgcttaaa aaacagccgt atcagcatca ttactactga agcaactgaa
     4381 ttgtataagt taatttaatg ttaagtagtg attcgtgccg gggcgatgtc tcgttttacc
     4441 cgaccgtcga agacaattat cagtctttat ccggcgttct aaggtgttta tcccactatc
     4501 acggctgaat cgttaatatt ttgcgagttc acgccgaaat actgattttt ggcgctagat
     4561 cacaggcata attttcagta cgttataggg cgtttgttac taatttattt taacggagta
     4621 acatttagct cgtacatgag cggcttgtgt ggctcctgac acaggcaaac catcatcaat
     4681 aaaaccgatg gaagggaata tcatgcgaat tggcatacca agagaacggt taaccaatga
     4741 aacccgtgtt gcagcaacgc caaaaacagt ggaacagctg ctgaaactgg gttttaccgt
     4801 cgcggtagag agcggcgcgg gtcaactggc aagttttgac gataaagcgt ttgtgcaagc
     4861 gggcgctgaa attgtagaag ggaatagcgt ctggcagtca gagatcattc tgaaggtcaa
     4921 tgcgccgtta gatgatgaaa ttgcgttact gaatcccggg acaacgctgg tgagttttat
     4981 ctggcctgcg cagaatccgg aattaatgca aaaacttgcg gaacgtaacg tgaccgtgat
     5041 ggcgatggac tctgtgccgc gaatctcacg cgcacaatcg ctggacgcac taagctcgat
     5101 ggcgaatatc gccggttatc gcgccattgt tgaagcggca catgaatttg ggcgcttctt
     5161 taccgggcaa atcactgcgg ccgggaaagt gccaccggca aaagtgatgg tgattggtgc
     5221 cggtgttgca ggtttggccg ccattggcgc agcaaacagt ctcggcgcga ttgtgcgtgc
     5281 attcgacacc cgcccggaag tgaaagaaca agttcaaagt atgggcgcgg aattcctcga
     5341 gctggatttt aacgaggaag cgggcagcgg cgatggctat gccaaagtga tgtcggacgc
     5401 gttcatcaaa gcggaaatgg aactctttgc cgcccaggca aaagaggtcg atatcattgt
     5461 caccaccgcg cttattccag gcaaaccagc gccgaagcta attacccgtg aaatggttga
     5521 ctccatgaag gcgggcagtg tgattgtcga cctggcagcc caaaacggtg gcaactgtga
     5581 atacatcgtg ccgggtgaaa tcttcagtac ggaaaatggt gtcaaagtga ttggttatac
     5641 cgatcttccg ggccgtctgc cgacgcaatc ctcacagctt tacggtacta acctcgttaa
     5701 tctgctgaaa ctgttgtgca aagagaaaga cggcaatatc actgttgatt ttgatgatgt
     5761 ggtgattcgc ggcgtgaccg tgatccgtgc gggcgaaatt acctggccgg caccgccgat
     5821 tcaggtatca gctcagccgc aggcggcaca aaaagcggca ccggaagtga aaactgagga
     5881 aaaatgtgcc tgctcaccgt ggcgtaaata cgcgttgatg gcgctggcaa tcatcctgtt
     5941 cggttggctg tcaagcgttg cgccgaaaga gtttcttggg cacttcaccg ttttcgcgct
     6001 ggcctgcgtt gtcggttatt acgtggtgtg gaatgtatcg cacgcgctgc atacaccgtt
     6061 gatgtcggtt accaacgcga tttcagggat tattgttgtc ggagcactgt tgcagattgg
     6121 ccagggaggc tgggttagct tccttagttt tatcgcggtg cttatagcca gcattaatat
     6181 tttcggtggc ttcaccgtga ctcagcgcat gctgaaaatg ttccgcaaaa attaaggggt
     6241 aacatatgtc tggaggatta gttacagctg catacattgt tgccgcgatc ctgtttatct
     6301 tcagtctggc cggtctttcg aaacatgaaa cgtctcgcca gggtaacaac ttcggtatcg
     6361 ccgggatggc gattgcgtta atcgcaacca tttttggacc ggatacgggt aatgttggct
     6421 ggatcttgct ggcgatggtc attggtgggg caattggtat ccggctggcg aagaaagttg
     6481 aaatgaccga aatgccagaa ctggtggcga tcctgcacag cttcgtgggt ctggcggcag
     6541 tgctggttgg ctttaacagt tatctgcatc atgacgcggg aatggcaccg attctggtca
     6601 atattcacct gacggaagtg ttcctcggta tcttcatcgg ggcggtaacg ttcacgggct
     6661 cggtggtggc gttcggcaaa ctgtgtggca agatttcgtc taaaccgttg atgctgccaa
     6721 accgtcacaa aatgaacctg gcggctctgg tcgtttcctt cttgctgctg attgtatttg
     6781 ttcgcacgga cagcgtcggc ctgcaagtgc tggcattgct gataatgacc gccattgcgc
     6841 tggtattcgg ctggcattta gtcgcctcca tcggtggtgc agatatgccg gtggtggtgt
     6901 cgatgctgaa ctcgtactcc ggctgggcgg ctgcggctgc gggctttatg ctcagcaacg
     6961 acctgctgat tgtgaccggt gcgctggtcg gttcttcggg ggctatcctt tcttacatta
     7021 tgtgtaaggc gatgaaccgt tcctttatca gcgttattgc gggtggtttc ggcaccgacg
     7081 gctcttctac tggcgatgat caggaagtgg gtgagcaccg cgaaatcacc gcagaagaga
     7141 cagcggaact gctgaaaaac tcccattcag tgatcattac tccggggtac ggcatggcag
     7201 tcgcgcaggc gcaatatcct gtcgctgaaa ttaccgagaa actccgcgct cgtagtatta
     7261 atgtgcgttt cggtatccac ccggttgcgg ggcgtttgcc tggacatatg aacgtattgc
     7321 tggctgaagc aaaagtaccg tatgacatcg tgctggaaat ggacgagatc aacgatgact
     7381 ttgctgatac cgataccgta ctggtgattg gtgctaacga tacggttaac ccggcggcgc
     7441 aggatgatcc gaagagtccg attgctggta tgcctgtgct ggaagtgtgg aaagcgcaga
     7501 acgtgattgt ctttaaacgt tcgatgaaca ctggctatgc tggtgtgcaa aacccgctgt
     7561 tcttcaagga aaacacccac atgctgtttg gtgacgccaa agccagcgtg gatgcaatcc
     7621 tgaaagctct gtaaccctga cggcctctgc ggaggccgtc actctttatt gagatcgctt
     7681 aacagaacgg cgatgctttg acctcccgct gtttgttcaa gcgcaatttt gacaataatt
     7741 gtcaacggca cggaaagcag catacccacc ggtcctaaca accatcccca aaaaatcaac
     7801 gacaaaaata ccaccaatgt cgaaagcccc agcccacgtc ccatgatgcg cggctccaga
     7861 atattgccga agaccagatt aatcagcaga tatcccgcca gcaccagcaa cgcttcgtag
     7921 aagccattaa acaccagtag ctgagcgata ggggggattg ccgcgagaac tgaaccaata
     7981 ttagggatgt aattaagcgc aaaggccagc aatccccaga caaaagcgaa gtgaacatcg
     8041 agtgcggcga gcatcgccca ggcgaccaag ccggtgatga tgctgatggc tgttttcagc
     8101 accagataat gagaaacact gtcaatcgca cgttgaatcg ccgccatccc ttcaaccgga
     8161 cgcgccatca tttgctgaaa ttttccgggc aattgtggca cttcgagcag cataaacagc
     8221 accgtcagca gcaataaaaa tattgatgac atggcattag ataactgcgt caataagttg
     8281 gtgagcaacg tcatcgccgc gttcggatca atgtaatgcg ccagttggtc aactgagacg
     8341 tcaatcccta cgcgttgcaa caacggctca agagcttgca gcggcgtcat aatagagttg
     8401 cgatattgcg gtaacgtccg cgtcaactcg ttgagcgcgg aacccagata agctaatagc
     8461 aacaccatcg ccatcacgat gattgtcatc aaaatcgaca ctgccagtac acgcggcaca
     8521 cgccagcgga ccatatgttg caccagcggg ttaagaataa cagcaataaa taatgcgaga
     8581 ataaacggca cgatgatctc ggcggcaaaa cggataccgc agagaataat gaccagcatt
     8641 cccaacataa tgacgatttt taggccattg agcgtgatga tcggctttgc catgcttgtc
     8701 cccgtttttc ttcctttgct aataataatg tctttttcgc tccgtcatca taacgaaaat
     8761 caaaccgctc aggtaaagaa gtgaaaatat tttgagttaa ttcttaagct atgatacaaa
     8821 tcaggcgtgt tcaactaccg aggacaatta tcatctgcga tgacgagaag caacactgcg
     8881 gataattgta atattatgga caatttgttc aggacgttat tttctacatt tacccatctt
     8941 cgtacctcat ccactatctt gctcgtaggt gagcagcact ggcgcaacgc gctttagtcc
     9001 tttcttctgc ctaagcttgc cctatgcgca gggcttgacc actttacttt ggtttcgctg
     9061 aattaagcga aaattaaaat agttctcttg caggagaagg acaatgtata tttattggat
     9121 tttattaggt ctggctattg ctacagaaat taccggtacg ctgtcaatga aatgggcgag
     9181 cgtcagtgag ggaaatggcg gctttatttt aatgctggtg atgatttctc tgtcgtatat
     9241 atttctctct ttcgccgtta aaaaaatcgc cttaggcgta gcttatgcgc tgtgggaagg
     9301 tatcggtatt ttatttatta ccttgtttag cgttttgtta ttcgacgaaa gtttatcgct
     9361 gatgaaaatt gccgggttaa ccaccctggt cgccgggata gtgttgatca aatcaggtac
     9421 ccgtaaagcg cgtaaacctg aactggaggt gaaccatggc gcagtttgaa tgggttcacg
     9481 ccgcctggct ggcattggca atcgtgctgg aaatcgttgc taacgtcttt ttgaaatttt
     9541 ctgacggctt tcgtcgcaaa atatttggct tgctctccct ggcggcggtg ctggcggcct
     9601 ttagtgcgct ttctcaggcc gttaaaggga tcgacttgtc tgtcgcttat gcattgtggg
     9661 gcgggtttgg tattgccgcc acgttagccg caggttggat cttgtttggt caacggttaa
     9721 atcgtaaagg ctggattggc ctggtcttgc tgttggctgg aatgatcatg gtgaaacttg
     9781 cctgatgaag acgctgcccg cgctgtcggg cagcgtttga acattatttt tgcgacagtt
     9841 gatccagctt gtcgcgaaaa ccggtaacag aaatggcccg gttatcggcg cgccagcgat
     9901 ctttcgcggc aggagccgaa ctttgcaccc caattaattg ccagccgtca tcggtatgca
     9961 acatcagagg cgaaccgctg tcacccggca aggtatcgca ctgatgtgac atcaccgacg
    10021 tttgcgccca gccagtcact tcacagtttt gatgactgta taacgtatcg agatgatctt
    10081 cagggtatcc cgcctgagtc actttacgac cagccgattt taatgcggca gtaagcgcgg
    10141 ctttatctcc ctcaaataac ggcaacggtg taatgccaga agggggatta cgtagcacaa
    10201 tcaatccgaa gtcccacggt gcagctgcgg gaggaacaat ccaaccatcc ccatctgctt
    10261 ttaaccgctt tcccagtgtc ggatcaacgc ggccttctat gtcgtggatc tcatagcgcc
    10321 aaagaccttt atttgacaca aaacgcagcg ctaccgcctt atccgcttta cccttaggcg
    10381 gcgtcaataa acagtgtcct gccgttaatg ccagattggg tgcaatcagc gtcgccgtac
    10441 ataaattgcc gctggccgtt tccagttgcc caaccgcatc ccacggtgat tgggtcgtgt
    10501 cattcactgg cacacgatca tcatgaccaa aaaacagggt gctgacctca tcgtttgccg
    10561 atttggcaac gtctggttta tctgcaaaca caaaagcaga cgtcaaacta attgcaccca
    10621 acactacagc aatggttgta cgcatatcac actctggtgg gtaattatga ttattaaaag
    10681 caaaccctca taaatactat agacgggacg ggatgaaagt gggagtaaaa tcagataact
    10741 acagtcagga aagataaaaa cgggcggcaa tcagtgcgca taaaataagg atgataagaa
    10801 tgaactcaaa gcgataacgg cccaccatac taccctccgc taaaggcggt gctaaacgtt
    10861 acccgcttag caccgtaatt ttcaggcgcg agggggcgcg ccagcattac tgttgaaaac
    10921 ttacgctgcg ggttgtgcag caggttttgc cggttgctga tggccgtgtt tcccggcgtg
    10981 tttcttcgct gcctgcgctt tttgttcagg ggctttctgt tcagctttcg tatttttatg
    11041 atgcttttta gccgcctgcg ctttctgggc aggtgctgct ttatgctgtt ttttatgatg
    11101 tgtagttttc gctggcgctg ctttggtggt cgtcgcagtc ggagcaggtg tggtcgcagt
    11161 ctctgcagca aaggcggcag aagacagacc catagcagcg gcaacaacca gagctaatac
    11221 ttttttcatt gtcataccct caatttgttt tttcatttaa ccccactgcg gggccgttga
    11281 aataactata tccctgggaa tggcagactt ccgtgagtgg ttggtttcag cgtgtaacga
    11341 tatgtacaaa cgctgaataa attacagcgt tgataataac gtattgtgac ttaaggaaaa
    11401 tttagctacc aataatagta gtcttgatcg ggttaactat tatactactt acaaagc
//
LOCUS       DAJVQH010000123        11447 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJVQH010000123 DAJVQH010000000
VERSION     DAJVQH010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.123"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            16..468
                     /locus_tag="OQM87_003370"
     CDS             16..468
                     /locus_tag="OQM87_003370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1509389.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYVFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            714..1758
                     /locus_tag="OQM87_003371"
                     /pseudo
     CDS             714..1758
                     /locus_tag="OQM87_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1820..2434
                     /locus_tag="OQM87_003372"
                     /pseudo
     CDS             1820..2434
                     /locus_tag="OQM87_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2491..3948)
                     /gene="pepD"
                     /locus_tag="OQM87_003373"
     CDS             complement(2491..3948)
                     /gene="pepD"
                     /locus_tag="OQM87_003373"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS1509390.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTITEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4210..4668
                     /gene="gpt"
                     /locus_tag="OQM87_003374"
     CDS             4210..4668
                     /gene="gpt"
                     /locus_tag="OQM87_003374"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1509391.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4760..6004
                     /gene="frsA"
                     /locus_tag="OQM87_003375"
     CDS             4760..6004
                     /gene="frsA"
                     /locus_tag="OQM87_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1509392.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6062..6463
                     /gene="crl"
                     /locus_tag="OQM87_003376"
     CDS             6062..6463
                     /gene="crl"
                     /locus_tag="OQM87_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1509393.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6502..7557)
                     /gene="phoE"
                     /locus_tag="OQM87_003377"
     CDS             complement(6502..7557)
                     /gene="phoE"
                     /locus_tag="OQM87_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCS1509394.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7845..8948
                     /gene="proB"
                     /locus_tag="OQM87_003378"
     CDS             7845..8948
                     /gene="proB"
                     /locus_tag="OQM87_003378"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS1509395.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8960..10213
                     /gene="proA"
                     /locus_tag="OQM87_003379"
     CDS             8960..10213
                     /gene="proA"
                     /locus_tag="OQM87_003379"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCS1509396.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            10328..10403
                     /locus_tag="OQM87_003380"
     tRNA            10328..10403
                     /locus_tag="OQM87_003380"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10361..10363,aa:Thr,seq:cgt)
     gene            complement(10418..>11447)
                     /locus_tag="OQM87_003381"
     CDS             complement(10418..>11447)
                     /locus_tag="OQM87_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1509397.1"
                     /translation="AWRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWML
                     HFREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTK
                     TRHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPV
                     VMFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQ
                     GLHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWA
                     SWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEE
                     NVVYLNAR"
BASE COUNT         2746 a         2839 c         2947 g         2915 t
ORIGIN      
        1 ttttgaattt atattatgaa taacatacaa ataagagatt atcagcctgg cgattttcag
       61 caactatgcg ctattttcat tagagcggtt acgatgaccg tcagtcagca ttattcacca
      121 caacaaattg ccgcctgggc gcagattgac gaatctcgct ggaaggagaa actcgcgaaa
      181 tcacaagtgc gggttgcagt cattaatgca aaactggtcg gctttattac ctgcgttgaa
      241 cactatatcg atatgttatt tgttgaccct gaatacaccc gccgtggggt tgccagcgct
      301 ttgttaaaac cttttattaa gtctgaatcc gaacttacgg tggacgcaag cataaccgca
      361 aaaccctttt ttgaacgtta tgtttttcag acagttaagc agcagcgcgt tgaatgccgg
      421 ggagcgtggt ttattaattt ttatatgcgt tataaaccgc aacattaaat ccagcttgca
      481 atgaaaataa cgcccgccta gtatgtgcgc gttttcttat ttcacaaact gcaaggaggt
      541 aaatcatgac aaatccttta tcaatgactc tttgcagacc tttccaggat taattctttt
      601 tttcttgccc tggattcgtc tgccatttcc tgatttttta attgttactc tcaataattc
      661 aagtcgcagc acatgcaact tgaagtatga cgagtctatt tacggaatgg tatatgggca
      721 attatattcg tcccttatcc gatgcggtat ttaccatcgc atctgatgac ctgtggatcg
      781 agagtttagc gatccaacaa ttacacacca cggcaaattt acccaacatg cagcgcgtag
      841 tggggatgcc agatttacac cccggacgcg gctacccgat tggcgcagcg ttcttctccg
      901 caggccgttt ttacccagca ctggtcggca atgatatcgg ctgtggcatg gcgctatggc
      961 aaacagatat tctcgctcgc aaatacaacg ccgataagtt tgaaaagcga ttatcagctt
     1021 tggatgacgt tgctgaagaa agctggctgg aggaaaacct gccgtcagca tttgcacagc
     1081 atccgtggcg cagctcgctt ggctctatcg gtggtggcaa tcatttcgct gaactgcaac
     1141 aagttgatca aatcatcaac gctgaactgt ttgcactggc aggtctggat gcgcagcatc
     1201 tgcaactgct ggttcatagc ggctcgaggg agctagggca gtctatttta cagcggcata
     1261 ttgcctcgtt ttcgcatcat ggtttgcctg aaggcagtga cgacgcgctg gcgtttgcgc
     1321 gttttaatcg ccacctgatc gctttgcgca taatgcaaca ggttaaggcc accggaagtc
     1381 cggttctgga tgtggcgcat aactttgtta gcgcgtgtcg aatcggtgat caacaagggg
     1441 tcttacatcg taaaggtgcc acgcctgatg actgtggtct ggtggttatt cccggttcac
     1501 gcggtgatta ctccaggctg gttcaggccc gtcaggagtg aggaaacatt gcattcgctg
     1561 gcgcatgggg ctgggcgtaa atgggggcgc accgagtgta aagggcgtct ggcagcgaaa
     1621 tacacagcga cgcaactctc gcgtactgaa cttggcagtc gggtaatttg tcgcgataaa
     1681 caactcatct tcgaagaagc gccacaagcc tataaatcgg ctgaaagtgt ggtgcaatgt
     1741 ctgggcaggc cggattaatt attcctgtcg cgcggctgcg tccggtgcta acgctcaaaa
     1801 acagtggagg gaaaaaagga tgatcttgct ataactctcc tcagcgcagg ggccggaaga
     1861 atgttgtctc gcagtgaaaa aggcgctgga caggctaatt aaagaagctg cccgacaaga
     1921 cgtcgcggta acggtgctgg aaacagaaac aggccgctac tctgacacgc tgcgttcggc
     1981 gctgatttct ctggatggcg acaacgcatg ggcgttaagc gaaagctggt gtggcactat
     2041 tcagtggatt tgcccgagcc cgtatcgccc acatcacggg cgtaaaaact ggtttctggg
     2101 cattgggcgt tttaccgctg atgagcagga gcaatccgat gcgatccggt atgagacgct
     2161 gcgttcgtcg gggccgggcg gtcaacatgt caataaaacc gactcggcgg tacgcgccac
     2221 gcatttggca tccggtatta gcgtgaaggt tcagtcagag cgcagccagc atgctaacaa
     2281 acggctggcg cgattgctga ttgcctggaa gctggatcag cagcaatagg aaaatagcgc
     2341 ggtgctgaaa tcacagcggc gaatgttcca tcaccagatt gaacgtggca acccgcgacg
     2401 aacgtttaca gggatggctt ttatcgaagg ataatgaagg atgaaactgc cggaaggcga
     2461 ttaaacgcca tccggcagca aatcaaataa ttacttcgcc ggaatttctt tcagcagttc
     2521 agtcagcagt gtccagtaat gacctacgct ttcgatgtga acttgctcat ccggagagtg
     2581 tggaccggtg atagttggcc cgatagaaac catgtccatt tccggatacg gttttttgaa
     2641 cagaccacat tccaggcccg cgtggataat ctggatgttc ggcgtcttgt tgaacaggcg
     2701 ctgataggtt tcacgtacca gatgcatcac cggagaatta gcgtccggct gccagccagg
     2761 atatgcgcct ttcgcttcgg ttttcgcgcc agccagttta cccagcgaat ccagcatgct
     2821 caccacgtag tctttaccgc tgtcgatcag tgaacggatc aggcagtgaa tttctacgtt
     2881 attgtcagtc atggtcacca caccgacgtt cagggaggtt tcgaccacac cttttgccac
     2941 gtcggaattg cggatcacac cgttcggggt ggcgttcagc agacgaataa aggtatcgcg
     3001 agatttcgca atcagggcag ctttatcgtt cgctacagag tccagcaaca aggccagatt
     3061 tttctctttc tctgccagct cgtttttcag gatctcctga taggtattca ccagagattt
     3121 cagtgcgtcg actttatcag ctgtgacagc aatggtcgca aaggcttcac gcgggatggc
     3181 gttacgcagt gtgccgccgt tgaaatcgat aaggcgcagg tccagttctt ctgcatgacc
     3241 cgccaggaag cgcaccagca gtttgttggc attacccagg ccaacgtgga tttcaccgcc
     3301 ggagtgaccg cctttcagac cttttaaggt taacttgaag gtttcaaaac cagctggaac
     3361 cgcttcacga tctaaatgca ggttggaggt gaagtcgata ccccccgcac aacccatgta
     3421 gatttcacct tcttcttcgg agtcggtgtt aatcagaata tcagcctgca accagttgct
     3481 ctgtaagccg aacgcaccgt ccataccggc ttcttcggtt atggtcagca gcacttccag
     3541 cgggccgtga accacgtttt cgtcagccag aaccgccagt gcagaggcca taccaatgcc
     3601 gttatccgca cccagcgtgg tgccgcgcgc tttaacccat tcgccatcaa tataaggctg
     3661 gataggatct ttcgtgaagt catgcacggt gtcgttattt ttctgcggca ccatatcgag
     3721 gtgggcctgt aagacgaccg gtttacgatt ttccatacct gcggtagcag gtttacgaat
     3781 caggatatta cctacctgat cgcgttcgac atggaaacct ttctcttttg cccaaccaac
     3841 aatgtattca gcgagttgct cttcatgata ggacgggtga ggaatagaac agattttggc
     3901 aaaaatatcc cacagcggct gtggagataa ttgagacagt tcagacacgt taagtctcct
     3961 tgtcgatcac ccgcaaaaca gtattgcagg tcacagggtt agcagaaaat gttgtcaaca
     4021 caagacaggc ttgcgagata tgtattgaga ataccacttt atcccgcgtc agggagaggc
     4081 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatatctat
     4141 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
     4201 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
     4261 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
     4321 ggcggtctgg taccgggggc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     4381 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     4441 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgct
     4501 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     4561 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gatcgaacag
     4621 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     4681 ctggcattgc cgggcgttgt tctttttaac ttcaggcagg ttacaatagt ttccagtaag
     4741 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     4801 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
     4861 tgtgcagtcg gctcttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     4921 gatgtggatc tggcgcggta ttgacccacg tgaaatcctc gacgtccagg cacgtattgt
     4981 gatgagcgat gctgaacgta ccgacgatga tttatacgat accgtgattg gctaccgtgg
     5041 tggcaactgg atttatgagt gggccaccca ggcgatggtg tggcaacaaa aagcctgtac
     5101 ggaagaagat ccgcaactca gtggtcgaca ctggctgcat gcggctacgt tgtacaacat
     5161 tgccgcctat cctcatctga aaggagatga cctggccgag caagcgcagg ctttatcaaa
     5221 ccgcgcctat gaagaggccg cccagcgtct gccgggcacg atgcggcaga tggagtttac
     5281 cgtacccggc ggtgcgccca tcaccggctt tttgcatatg ccgcaaggcg atggcccgtt
     5341 cccgacagta ttaatgtgtg gtggtctgga tgcgatgcag acggactatt acagcctgta
     5401 tgaacgttat tttgcgccgc gcggcattgc gatgctgact attgatatgc cgtcggtggg
     5461 cttttcttca aaatggaagc tcacccagga ctccagcctg ttgcatcagc acgtcttaaa
     5521 ggcgctgcct aacgtaccgt gggtggatca cactcgcgtc gcggcctttg gtttccgttt
     5581 cggcgctaac gttgccgtgc gtctggcata ccttgaatcg ccgcgtctga aagcggttgc
     5641 ctgtcttggt ccggtagttc ataccctgtt gagtgatttt aagtgccagc aacaggtgcc
     5701 ggaaatgtat cttgacgttc tggcgagtcg tttggggatg catgatgctt ccgacgaagc
     5761 gttacgcgtg gagctgaatc gctattcatt aaaagtgcaa ggattgctgg ggcgtcgctg
     5821 cccaacgcca atgttatcag gctactggaa gaacgatccg ttcagcccgg aagaggactc
     5881 acgcttaatc acctcatcat ctgctgacgg taaattatta gagatcccat ttaacccggt
     5941 gtatcggaat tttgacaaag gtcttcagga aatcaccggc tggatcgaaa aacgcttgtg
     6001 ttaaaaattt gctaaatttt gccaatttgg taaaacagtt gcatcacaac aggagatagc
     6061 aatgacgtta ccgagtggac acccgaagag cagattgatc aaaaaattta ccgcactagg
     6121 cccgtatatt cgtgaaggta agtgcgaaga taatcgattc tttttcgatt gtctggctgt
     6181 atgcgtcaac gtgaaaccgg caccggaagt gcgtgaattc tggggctggt ggatggagct
     6241 ggaagcgcag gaatcccgtt ttacctacag ctaccagttt ggtctgttcg ataaagcagg
     6301 cgactggaag agtgttccgg taaaagatac tgaagtggtt gaacgactgg agcacaccct
     6361 gcgtgagttt cacgagaagc tgcgtgaact gctgacgacg ctgaatctga agctggaacc
     6421 ggcggatgat tttcgtgatg aaccggtgaa gttaacggcg tgagtgaaat gtgccggatg
     6481 catcacatcc ggcaatattc attaaaactg atacgtcatc ccaaccgcga cagtatcatc
     6541 attattaata ttcagcttat tatcgctatc cagtttgttg attttataat caacaaacgc
     6601 tgacatattt ttgttgaaat aatacgtagc accgacgtcg atataattga ccagatcttc
     6661 atcaccgatg ccctcaatat ctttcccttt cgataagaca taacccagcg atggacgcag
     6721 accaaagtca aactggtatt gagcgaccgc ttcaaagttc tgtgtcttat tggcaaagcc
     6781 gccagttatt ggcgtcattt tgcgtgtttc agaatagaaa gttgccagat aaatattatt
     6841 ggcatcgtat ttcagacctg ttgcccatgc ttctgcacgc ttgcctgtgc cacggctttg
     6901 caggttctgc tcgttggtgc gatctgagtt ggtataggcc ccactaatgg cgaaatcgct
     6961 gccgccaaag tcatatgtca atgacgtgcc gaagccatcg ccgttttgct ttttaacgtc
     7021 gcggttttcg tttttccctt gatattgcag ggttaagttc aggccatcga taacgccgaa
     7081 gaagtcggtg ttccgatacg tcgccagacc gctggcgcgt ttggtcataa agttgtcggt
     7141 ctgcgccgag gaatcgccac caaattccgg gaacatatcg gtccaggctt ccacgtcata
     7201 caacgcgccc aggttacgac cataatcgaa agaacccaaa tctttatatt tcaacccggc
     7261 aaaagcgaga cgcgtttttt gctgtgcagt atcactctcc gctttatttc cggcaaactc
     7321 cgcttcccac cgaccataac cagtcagttg atcgttaatt tgagtttcac ctttaaaacc
     7381 aaaacggata taactctggt cgccatcttt actggcgtta tcactcatat aatgcatggc
     7441 tttaactttg ccatagacat ccagtttatt accgtcttta ttatatattt ctgcagcctg
     7501 tacagatgca gatgccacaa tgcccatcac cactaatgcc agagtgctct ttttcatttt
     7561 cattcctgat tttaattaac gcgcgaatat tcagcgggag agccccgttg aaaacaggaa
     7621 agtttttaac ctgagattgt taaagatata ttacagatta atgatattct aaaaatgtgg
     7681 taatttatta aatctgtaat aaaagcgtaa acaactgccg ctacgcttgc tgatcccgcg
     7741 caacaaaacg ccatgctttg ctcgcagatg gttggcaacc gacgacagtc ctgctaaaac
     7801 gttcgtttga tatcattttt cctaaaattg aatggcagag aatcatgagt gacagccaga
     7861 cgctggtggt aaaactcggc accagtgtgc taacaggcgg atcgcgccgt ctgaaccgtg
     7921 cccatatcgt tgaacttgtt cgccagtgcg cgcagttaca tgccgccggg catcggattg
     7981 ttattgtgac gtcgggcgcg atcgccgccg gacgtgagca cctgggttac ccggaactgc
     8041 cagcgactat cgcctcgaaa caactgctgg cggcggtagg gcagagtcga ctgattcaac
     8101 tgtgggaaca gctgttttcg atttatggca ttcacgtcgg gcaaatgctg ctgacccgtg
     8161 ctgatatgga agaccgtgaa cgcttcctga acgcccgcga caccctgcgt gcgttgctcg
     8221 atcacaatat cgttccggta atcaatgaga acgatgctgt cgctacggca gagattaagg
     8281 tcggcgataa cgataacctt tctgcgctgg cggcgattct ggcgggtgcc gataaactgt
     8341 tgctgctgac cgatcaaaaa ggtttgtata ccgctgatcc gcgcagcaat ccgcaggcag
     8401 aactgattaa agatgtttac ggcattgatg acgcactgcg cgcgattgcc ggtgacagcg
     8461 tttcaggcct cggaactggc ggcatgagta ctaaattgca ggccgctgac gtggcttgcc
     8521 gtgcgggtat cgacaccatt attgccgcgg gcagcaagcc gggcgttatt ggtgatgtga
     8581 tggaaggcat ttccgtcggt acgctgttcc atgcccaggc gactccgctt gaaaaccgta
     8641 aacgctggat tttcggtgcg ccgccggcgg gtgaaatcac ggtagatgaa ggggcaactg
     8701 ccgccattct ggaacgcggc agctccctgt tgccgaaagg cattaaaagc gtgactggca
     8761 atttctcgcg tggtgaagtc atccgcattt gcaacctcga aggtcgcgat atcgcccacg
     8821 gtgtcagtcg ttacaacagc gatgcattac gccgtattgc cggacaccac tcgcaagaaa
     8881 ttgatgcaat actgggatat gaatacggcc cggttgccgt tcaccgtgat gacatgatta
     8941 cccgttaagg agctggccga tgctggaaca aatgggcatt gccgcgaagc aagcctcgta
     9001 taaattagcg caactctcca gccgcgaaaa aaatcgcgtg ctggaaaaaa tcgccgatga
     9061 actggaagca caaagcgaaa tcatcctcaa cgctaacgcc caggatgttg ctgacgcgcg
     9121 agccaatggc cttagcgaag cgatgcttga ccgtctggca ctgacgcccg cacggctgaa
     9181 aggcattgcc gacgatgtac gtcaggtgtg caacctcgcc gatccggtgg ggcaggtaat
     9241 cgatggcggc gtactggaca gcggcctgcg tcttgagcgt cgtcgcgtac cgctgggggt
     9301 tattggcgtg atttatgaag cgcgcccgaa cgtgacggtt gatgtcgctt cgctgtgcct
     9361 gaaaaccggt aatgcggtga tcctgcgtgg tggtaaagaa acctgtcgca ctaacgcggc
     9421 aacggtggcg gtgattcagg acgccctgaa atcctgtggc ttaccggcgg gtgccgtgca
     9481 ggcgattgat aatcctgacc gtgcgctggt cagtgaaatg ctgcgtatgg ataaatacat
     9541 cgacatgctg atcccgcgtg gtggcgctgg tttgcataaa ctgtgccgtg aacagtcgac
     9601 aatcccggtg atcacaggtg gtataggcgt atgccatatt tacgttgatg aaagcgcaga
     9661 gatcgctgaa gcattaaaag tgatcgtcaa cgcgaaaact cagcgtccga gcacatgtaa
     9721 tacggttgaa acgttgctgg tgaataaaaa catcgccgat agcttcctgc ccgcattaag
     9781 caaacaaatg gcggaaagcg gcgtgacatt acacgcagat gcagctgcgc tgacacagtt
     9841 gcaggcaggc cctgcgaagg tggtggcggt taaagccgaa gagtatgacg atgagtttct
     9901 gtcattagat ttgaacgtca aaatcgtcag cgatcttgac gatgccatcg cccatattcg
     9961 tgaacacggc acacaacact ccgatgcgat cctgacccgc gatatgcgca acgcccagcg
    10021 ttttgttaac gaagtggatt cgtccgctgt ttacgttaac gcctctacgc gttttaccga
    10081 cggcggccag tttggtctgg gtgcggaagt ggcggtaagc acacaaaaac tccacgcgcg
    10141 tggcccaatg gggctggaag cactgaccac ttacaagtgg atcggcattg gtgattacac
    10201 cattcgtgcg taaataaaac cgggtgatgc aaaagtagcc atttgattca caaggccatt
    10261 gacgcatcgc ccggttagtt ttaaccttgt ccaccgtgat tcacgttcgt gaacatgtcc
    10321 tttcagggcc gatatagctc agttggtaga gcagcgcatt cgtaatgcga aggtcgtagg
    10381 ttcgactcct attatcggca ccatttaaat caataagtta cctcgcattt aagtaaacca
    10441 cgttctcctc ttgtgccgta tttgtgccat tgcgacttat aatcgcatcg attttgcttg
    10501 cgtgctcggt gagatgcccg gctgaaaggt gggcgtatct ttgaaccatt tcgagagttt
    10561 cccatcctcc catctcttta agtgcaagaa gagagacacc ggactgaacc agccagcttg
    10621 cccaggtatg cctcagatca tggaagcgga agttgctaat gcctgcccgc tttaacgctc
    10681 ccttccatgc cttgttgctg tcggttctca tcttccttac cgctgctgtt tttgttccgt
    10741 cgcttcggta ggcaggtttg gtgtggacaa atacccatct cttatggagc ccctgctgtt
    10801 ttcttaatat ctggcatgcg gtttcgttaa gaggaactcc gatcgcattg ccagcttttg
    10861 tttcatcagg gtgcatccat gccattttct tatccagatc gacctgtgac cactcaaggt
    10921 ctgtaacgtt ggaacggcga aggcctgtcg tgattgcaaa catgaccaca gggaagaagt
    10981 gaggagcaat ttctgcaaac aggcgcttcg attcctcctc tgtaagccat ctgatgcgtc
    11041 cattcttaac gcgtggtgtt gatattttgg gtgccctgtc aagccatccc cattcaacag
    11101 ccatattgag aatggcgcga agtattgcca gatgcctcgt cttcgttcct ttgcttgcaa
    11161 gctttggttt atactccggc accggattgc caagtcgcaa acacctgtcc cggctcatct
    11221 cccagttcag gcgatggcgg cggttttcca tcccgtctac cgcctccatt attttttctg
    11281 ttgttatgtc agagagaatg gcttctctga agtgcaacat ccagaacgat ataatgctct
    11341 tgtcatcatc aatggacttc ttatccgatt tctcacgcag ccaccgtatg caggcttcct
    11401 tgaatagctt tttcggtgat tccccgagat tttttactct ccacgct
//
LOCUS       DAJVQH010000124        11365 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJVQH010000124 DAJVQH010000000
VERSION     DAJVQH010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.124"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            2..304
                     /locus_tag="OQM87_003382"
     CDS             2..304
                     /locus_tag="OQM87_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509398.1"
                     /translation="MKVNETDAEYLVRKFDALEAKCAAQENKVIPVSTELPPANESVL
                     LFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLENVNITHWAVMPKAPEAGA"
     gene            304..609
                     /locus_tag="OQM87_003383"
     CDS             304..609
                     /locus_tag="OQM87_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509399.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGNNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            836..1999
                     /locus_tag="OQM87_003384"
     CDS             836..1999
                     /locus_tag="OQM87_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1509400.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVVTKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKESTNDV"
     gene            2349..2711
                     /locus_tag="OQM87_003385"
     CDS             2349..2711
                     /locus_tag="OQM87_003385"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HCS1509401.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            2708..3625
                     /locus_tag="OQM87_003386"
     CDS             2708..3625
                     /locus_tag="OQM87_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCS1509402.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            3638..4903
                     /locus_tag="OQM87_003387"
     CDS             3638..4903
                     /locus_tag="OQM87_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HCS1509403.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(4970..5593)
                     /locus_tag="OQM87_003388"
     CDS             complement(4970..5593)
                     /locus_tag="OQM87_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1509404.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(6344..6682)
                     /locus_tag="OQM87_003389"
                     /pseudo
     CDS             complement(6344..6682)
                     /locus_tag="OQM87_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            6804..7064
                     /locus_tag="OQM87_003390"
     CDS             6804..7064
                     /locus_tag="OQM87_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCS1509405.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(7082..9817)
                     /locus_tag="OQM87_003391"
                     /pseudo
     CDS             complement(7082..9817)
                     /locus_tag="OQM87_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(9818..11149)
                     /locus_tag="OQM87_003392"
     CDS             complement(9818..11149)
                     /locus_tag="OQM87_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCS1509406.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPNVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPQDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMLTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            complement(11159..>11365)
                     /locus_tag="OQM87_003393"
     CDS             complement(11159..>11365)
                     /locus_tag="OQM87_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCS1509407.1"
                     /translation="YANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLA
                     SLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT         3078 a         2603 c         2507 g         3177 t
ORIGIN      
        1 tctgaaagta aatgaaacag atgctgaata cctggtgcgt aaattcgatg cgcttgaagc
       61 taaatgtgca gcacaggaaa acaaagtaat accagtgtca actgaactgc caccagcaaa
      121 tgaaagtgtt ttgttattcg atgctaacgg agaaggctgg ctaattggtt ggcgttctct
      181 ctggtacacc tggggacaaa aagaaaccgg agaatggcag tggacatttc aggtcgggga
      241 ccttgaaaac gtcaatatca ctcactgggc agtaatgcca aaagcaccgg aggctggagc
      301 ataatgacca cttttaccga caaagaactg attaaagaaa ttaaagagcg tatcagcagc
      361 cttgacgtgc gagacgatat tgagcgccgt gcttatgaaa tcgcactcct atctctggaa
      421 gtagaaccag atgaacgcga agcttatgaa ttattcatgg aaaagcgttt cggtgactta
      481 gtagatcgtc ggagagcaaa aaacggcaat aacgaataca tggcatggga tatgactctc
      541 ggttggatcg tctggcagca acgagctggt atccattttt caacaatgtc acagcaagag
      601 gtgaaataat ggagccatac agcctcacac tcgatgaggc ctgtcatttt ctcaagatat
      661 ccagaccgac tgccattaac tggatacgca cagggcgtct tcaggcaaca cgcaaagatc
      721 ccactaagaa taaatctcct tacctcacaa cacgacaagc ctgcattgcg gctcttcagt
      781 ctccgctgca tactgtccag gtgagcgcgg gtgatggcat aacagaggaa agaaaatgtc
      841 actcttccgc agaggtgaaa tatggtacgc cagtttcaca ttgccgaacg gtaaaagatt
      901 taaacagtct cttggaacaa aggacaaaag gcaggcgaca gaactccatg acaagctaaa
      961 ggctgaagca tggcgggtca gcaaacttgg tgaaatacct gatataacgt tcgaggaagc
     1021 gtgtgtcagg tggcttgaag agaaagcaca taaaaaatca ctggacgatg acaaaagccg
     1081 gatcggattc tggcttcaac atttcgcagg aatgcaacta agagacatta ctgaatcaaa
     1141 aatttattca gcaatgcaga aaatgacgaa ccggcgtcat gaggaaaact ggaaactcag
     1201 ggcagaagca tgcagaaaaa aagggaaacc tgttccagaa tacacgccaa aaccagcgtc
     1261 cgttgtaacg aaggctacgc atctttcatt tataaaggcc ctactaagag ccgcagagcg
     1321 tgaatggaaa atgctggata aggcaccaat tattaaagtg cctcaaccaa agaataaacg
     1381 gatccgctgg ctggagcccc atgaagcaca aaggctgatt gatgaatgtc cggagccatt
     1441 aaagtctgtt gttgaatttg cactggcaac aggcttaaga cgctcgaaca tcatcaacct
     1501 tgaatggcaa caaatagata tgcagcgccg ggtggcatgg ataaacccgg aagagagtaa
     1561 atcaaaccgc gcaatcggcg ttgcgctgaa tgatactgca tgtcgcgttt tgaaaaaaca
     1621 aatcggaaat catcaccgtt gggtatttgt gtacaaggaa agctgtacca aaccagacgg
     1681 aacgaaagcg ccaacagtaa ggaagatgcg gtatgacgca aacacagcct ggaaagcggc
     1741 gctgagacgg gctggtattg atgatttcag atttcacgac ttgagacaca cctgggcaag
     1801 ttggctggtt caagccggag tcccgttgtc agtgttacag gaaatgggag gctgggagtc
     1861 tatcgaaatg gttcgtcgat atgctcacct tgcacctaat caccttaccg aacacgcacg
     1921 gcaaatagac tcgatcctga acccatcggt cccaaatttg tcccagtcaa aaaataagga
     1981 aagtactaat gatgtgtaac ttattgattt aaatggtgcc gataatagga gtcgaaccta
     2041 cgaccttcgc attacgaatc tgtagcacca atcataacta tctgttttag caagcattaa
     2101 ccgcattcac taagatatag ttgatggcac aaacagaaag ttgatgaatg atgttgccat
     2161 atatatgtca caaatacggc acaacgatct tcaaacatat agccacacat tcacagaaga
     2221 gcacaaagcc ttgcaatcca gtgcaaagat ttgtgtgcca gagtttttct catcaactac
     2281 cgcaagtatc gatcgattga gacttggatg atagacttca tgcctttgat tattagctga
     2341 tagaagaaat gttaaagcta tttgcaaagt acacatctat aggcgtgctg aacacactta
     2401 tacactgggt ggtttttggt gtatgtatct atgccgcgca taccaatcag gctatggcaa
     2461 acttcgcagg tttcgttgtg gctgtgagtt ttagcttctt tgcgaatgca aaattcacat
     2521 tcaaagcatc aactacaacg atgcgctaca tgttatatgt cgggttcatg ggaacactga
     2581 gtgctactgt tggatgggtt gctgatagat gttcacttcc tccaattgtc actcttgtta
     2641 ccttctccgc catcagcttg gtgtgcggtt tcgtctattc aaagttcatt gtctttaggg
     2701 atgcgaaatg aagatatctc ttgtcgttcc tgtcttcaat gaagaagaag cgataccaat
     2761 tttttataaa acggtacgtg aattcgaaga gttgaagcca tatgaagtag aaattgtttt
     2821 cataaatgac ggaagcaaag acgctacgga gtcaatcatt aacgctctgg ctgtttctga
     2881 tccgctagtt gttccgctgt catttacacg caactttggt aaagaaccag cattgtttgc
     2941 agggttagac catacaaccg gcgatgccgt gattccaatt gatgttgacc tgcaagaccc
     3001 gattgaggtt attcctcatc ttattgaaaa gtggcaggca ggtgctgaca tggtgcttgc
     3061 taaacgttca gaccgctcaa ctgatggacg actgaaacgt aagacagctg agtggttcta
     3121 taaattacac aacaaaataa gcaccccaaa aatagaggaa aatgtcggag attttcgact
     3181 catgtctcgt gaggttgtgg agaacattaa actattgcct gagcgcaatc ttttcatgaa
     3241 aggcatactg agctgggttg gtggtcagac agatgtcgtt gaatatgtac gcacagagcg
     3301 tgttgctggc atctcaaaat ttaatggctg gaaattatgg aatctggcac tggaaggtat
     3361 cacaagtttt tcaacctttc ctcttcgcgt atggacttat ataggcttgt ttgttgcaag
     3421 catttcattt ttatatggtg catggatgat tatagacacc attgtctttg gtaacccagt
     3481 acgcgggtat ccctccatgc ttgtatcaat acttttcctg ggtggagtgc aactgatcgg
     3541 gattggtgtt ctcggtgaat atataggtag aatttattta gaaacaaaat caagaccacg
     3601 gtacttaatt aaaagcagaa aataacagga tgatcttatg aagatattca taatgcgcaa
     3661 ttggcacaag atatcaattt ttatacttgc atttactttg atttggctta ggcgcattga
     3721 cattttgaca aatgcccagt tttgggctga ggatgcagtt ttttggtata aagatgcata
     3781 cgaacaaggt tttttcagtt cactcataac cccaagaaat ggttattttc aaacagtatc
     3841 aacactcatt gttggtgcga cgacatttat taatccaatt tacgcaccat tattatcaaa
     3901 cttttttgga ataataatta gagctataat tatatggttc ttatttaccg atagatttaa
     3961 attcctaagc accacatcaa aaatcttcat ctctgtatat ttaatttgca tgcccgggct
     4021 tgatgaagtc caagccaata taacaaatgc tcactggtat ttatcattgt atgttgcaat
     4081 gataataata tctgaagaat ccaagtcaaa attatggaaa gcacatgatt tattttttat
     4141 agtattatct ggattgagcg gtccatttat tatattcata attgcatcat ctgtttttaa
     4201 atatttacac ttatcaaaag gagtaataag cataagaggt ttatacttat tttatactcg
     4261 actgccatat ttggcaatga tcttgtgtgg gctgatacag gcaacatcaa taattcttac
     4321 atttaatgga acaagatcac atgctccatt aggattcagt tttgatgtta tgtcatctat
     4381 aatatcatct aatgtatttc ttttttcatt tgtcccttgg aatatcgcta atgctggatg
     4441 ggataatcac cttttatcgt acacactatc aactttagta attacattat gtgttttcat
     4501 ttatattaaa ggaaattggc aaatgaaagt gtttgccacc cttccaatac ttattgtcgc
     4561 attttctatg gcaaaacctc aactggcaga ttctgttcct caactgccaa cacttgcaac
     4621 cggaaacggg tcaaggtatt ttgtaaatat tcatattgca attttttcat taatatgcgt
     4681 ttgtttgttt caatgcataa agaataaaac cttaaaaata ttctttaaaa tctatgtttc
     4741 agtcatcctt attgcaatga taaaattaaa cttttttata acccctcttc ctgacatgaa
     4801 ctggtctcaa ggagcagaat taataaataa agcaaaacat ggagaagccg tctcatttac
     4861 agtgctccct ccatggttaa ctcttgattt aataaaaaaa taaaacttca cattaataaa
     4921 taacaatgaa tccgcctccc tacgtattcc cgtagggagg ctgtcgtgtt tatagtagcc
     4981 tggtaatgct aaatgcaaac gaactggaga ttcctgctcc gctaagcgta ttgcgcaggg
     5041 tcaccctaaa tgtatctaca ccaatataag taacatcaca actaactgaa tcaggcgcag
     5101 atataggtgt tatctgaacc acataaccat tagtagctgc tgcgagcgga tgagaaaacg
     5161 ttacatcata cacaccagta gcatgcgcgt taacggatga aacacccggc gcaatgtcta
     5221 cactaccatt ggcgttaatt ctccctgagc gcgtaactga ctctgcagaa accgatgtgg
     5281 ccttactggt tatttccata aaggtgcaac caatatcgat caattcgcaa ttgttccctg
     5341 ttatccagta tggtcgatct gcacttggct gataatcccc tgcgctctgg aacgttgtgc
     5401 cattgagtaa gactataact tttcccccac ctgagcttgt tacttgtata ttcgtgtgcg
     5461 tgtagcgttc actgctcacg cgatgaaaat ttccaccgtt tataactacc gttacaggtc
     5521 gcgtcccagt attatctata gcaaggtctg cccccccggc attagcctcg aagtatgggt
     5581 tgtttaacac caaagcacac gaaccgttaa ggccatttat ggctaattgc gcaccacctg
     5641 taccggaatc cccctgagta ccatttcctt cacaagtcag gctgttgata gtagttcctg
     5701 ccccccatct atcaccaagt ataccccatc gtgtattctg gcttgcagtt aatcggttaa
     5761 ctgtaatggc attaagctct gacccttcac tggagttaag tgcgcgccag ccaatacggt
     5821 taccctgtaa gtacacatta tctaactctg acgttaacac cgagtctgta cgcaaccctt
     5881 cgccggtagt gtgcccataa gacacaatgt tctctaattt agtgtatgct ttattggtta
     5941 cgagaagacc gtaagacgat cccgaacctt taagtgtaat gtcccccaga taatcatagg
     6001 cgtgtacacg ttgggttgcc ccgactgtat caccgagcat ctttaagttg aaatccgagg
     6061 catggcagtt aaatatcgtt tctgccatat tttctccgga ataatgagca cggactcctg
     6121 tcgaaccaat aaatttagcg tcatagttgc cgtaatcaag aacgagtggt gacgcgatat
     6181 cgtacacccc ttgctttatt tttaatgttc caccgaaagg ccacgatgta agcgggtctt
     6241 gatatatttc attttttatt gcaaatagct cctcttggag atttttgccg ctattagtgc
     6301 caatgtgagc agctccttca tctgattgaa gtaactgctt taactgatca ggatcgtact
     6361 tcagcacatt ctgaaaatag aactgctgcg aaccatacgc atcatataca gccattgaat
     6421 ggccttgctc agttacaaat ttggcaatct gcccgttgta cacaggatat ccggcagcat
     6481 tgataacaat tggctgagaa acagggacgt gagatccgtc ttcattctct acataaacct
     6541 gaatctggtt ttcaggattt accgggtcag tgtcaatttt tccgatataa attttgccat
     6601 tggctacggc tttaaaagaa cgcgccatag tgaagagttg cgaaggcatg ctgaccacaa
     6661 catttgcggc gatatctgac atttcattgc tccagacgaa tgatatgatg caatcatgat
     6721 gtgattgcat accgaaatgg cactattgaa tatttatcca gtaggttacg atgccattcc
     6781 acccaactgg tgaggcatca aggatgtaca gcaaatacga cgaggcacag tttcacttga
     6841 gacttccgca tgaactccac gcgaaaatta aacagtgtgc gaagatgaat aacagttcgc
     6901 tgaactcaga gataattgca gcgattgaag aatcattggc taaacaaagc tctgcatcag
     6961 tttacattga cgatgcagag cgtatggcag aacaacaatc tgatatggtt aagaaaattg
     7021 tctttgatac gctcaaggag ctatataaaa aagacagcaa ctaaccatcc gtggctttcc
     7081 gttacttctg gttatcatca ctgttcatcc agtacatgat gcctagcctt gatatggttt
     7141 tcttatctga ttctggaagt gagttgtaaa acgagcgcca agcagaagag cgtcttacag
     7201 ttgtatcaac gatgctttct ggtgcttgtt ttactgccag ccttgctgct ttctggaatt
     7261 ctggtgagct aattagcttt tctgcagatt caattgattc agcgccgccc atcgccctga
     7321 ttttgctggc gacttttgcc ccaatttctg acccagcaac agcacctact gcactgaacg
     7381 gtcctgacac agttgaacca accgcagtgc caatgcgttg ggcatggtta gcaacaaatt
     7441 catttgccgc agtgacgcga ttaaaccgtt tgacgaactc gtttagtctt cctgtagtta
     7501 tctcgtaaga ttttgcatct ttgatagcct ttgcgacctt atatacatcg ttcagacctg
     7561 acatagtctc ttttggtaaa tgtctggcaa gattgcgcat ctgcccgttt gctaacatgt
     7621 tttgatacca gtcagcaaac cctgcaggat taaaatcagc gccacgtttt ccgttcgaaa
     7681 gcatatcccg aagacctgtt cccagtagct cggttcttag cttcctggac ggcgtgtttt
     7741 gcatcaattc acgaaatcct ttggcatcgc ctttcgacat tgcctgtagt gctgtagttg
     7801 ctttgcgaga aacatcaccg ttcagcgttc tgccagttag attaatcatt tgctcttcca
     7861 tgctttttcg catctgaaca agcctctgag caacttcaaa atcacgtaat gcatttgtct
     7921 ctgacagcgc cgccttttga tcatcggcga gcgaaccgta aagcctcgat aaagcagcgc
     7981 gatcagcatc tttatatggt ccagaattct tatgaagtgc atcaccaaca agtcgtcgtt
     8041 gcttattgag atttgcataa gttaatgcgc cgttcttgcc tggattaact gcccgaaaga
     8101 ctctcttttc aatagggtca aggtttttca tcccgccaag atcttctgcc acctgtttga
     8161 gcattgacct tgttgatggc gcatcaattt ttgcacctgc aggcatcgcc ttttcaacat
     8221 tgcgataaag ctggtcgctt cgtttcatca gcgcagacat tcttccattg accgtatcaa
     8281 taaacttgtc gctcatacca agagcatctt ttgcaccgga aacgcgatca attatctcac
     8341 ccgcgccctg tgctagctgc ctgattgctt cattttcctg cacctgtaga gcagaacccg
     8401 cacgggactt gattgcttgc tcaactgcct tgtattgctc gttcccggaa aagtgtgaag
     8461 ggagtaatga atcaacatta agcctttcag cagactccag aacttcagcc tgaggattga
     8521 tatctagttc atcaagcgat gaagcgagat ttggcctttt ttgtgctgct actgtgcgaa
     8581 cgacttcctc tgggttcttc gtcgcaggcg tcattggtac accttgtgct actcttgcct
     8641 ctgcggcatg aaccgcagga tttgttgctg cagcggcatc accggagaat ggtgatgttt
     8701 ctgttggcgc agtggtcgct ctctgtgcag atgttttatt gccactaatt atatttgatg
     8761 caccacgaac accacgggta acaccattta tcaggccatg cgtagcaatg ctaagtccgg
     8821 cattaatagc ggcattttcg gcaaaatcac cccgttgatt cgccgcgtca gcgagagaac
     8881 caatgaccat gtttccagct acacctgctc caggaacaag ataaccgcct attgactctc
     8941 cagcttgcgc gtaggggtct gtcggtctgt ctactggacg ataaacatca tccaatactt
     9001 ttggcccccc aagcccctga ctgattgcat taatcagact tgcgccaccc tgcaatacgt
     9061 caaatggtat gtttaccaga ccacgaccag cctgttctgc aatttaccct gcactttgac
     9121 caccagtgag ccaatcgcca gcttgttgca tcaatgatgg ttcttcccgt gttggtgcat
     9181 tattggcctg attaactgtt tgttgctgaa caggtttcgg tgcgacagaa cttacgggtt
     9241 gaggtggctg ctgactggct gcctgctgct caatctgagc aaatggatta tttgggtctg
     9301 actgcacacc tgatgccgat acttgttcgg atgactgtac ttccagttgt gcaaacgggt
     9361 tgttagggtc ttgctgagga tgtacctttg cagaggtggc gcgctgttcg actgttgagt
     9421 ctgtcaccgg gtcaccagcc cattgagcaa agcgatcatc aacgtaaccg cggccttcag
     9481 gtcctggcgt atattcacca cgctttgcct tcataacgtt gccgggaccg tcgtgataag
     9541 cctgaagagc gtcacgccag ttaccaaact gctggtatat ctttgccaga tagcgcgcgc
     9601 cagcgtcagc ctgatattcg gggttttgca tttgctcatc ggtataaccc atatcacgcc
     9661 atgtcccagg catgacctga gtcaatccta cagccccggc ggagcttact gcggcagggt
     9721 tgtaagaaga ctccttggca cccagtgcag tcatcaaccc ttctggcaca ccgtaacgtg
     9781 cgccagcctg ctctaacaaa tcacggtaat tagccattta ctgccccaaa gatggaagat
     9841 atccgtagcg attaatgaag tcgattgaca gctcggggtg ctgcttcagg taatctatag
     9901 aagcctgagg cgcttccact cgcttgatac cgttttgctg aacgtactta ccaaccgcct
     9961 cattacgctt ctggttgagc gtgttcagga tgacgccagc gttgcgacga aaggactcct
    10021 cgctctgcga gttctgcagc gaaccaacag cctggtcgag ctttttgccc tcggcatcag
    10081 aaagtgcgcc catgcctcgc atggcctgaa ccgctgtcag gtatgcttgg gatttaaagg
    10141 tatcaagtcg tgcctgagtg tctgcagcct gtgagcctgg aacgttgggg attactccac
    10201 gtaagcctgt aatgctctta agtgaaggag aactaacgat atcgttcaga gttaacatgc
    10261 tggttgtgag ggtgttgatg ccgtctttgt agccatcatt tagcgcttgc tgcttctgct
    10321 gcaactgctg gttgttggct gctatgcggc tctgtatttc ctggcgcttc aggtcgttag
    10381 tttctgctga tagcatccgg tcaaggcgct tattttcgtt gttaatgcgg tttgtttctg
    10441 cgtccagatt aatgcgctgc tgacctaaat tcgcctggat atcttgcccg cgcattgtga
    10501 ttgcctgatt ccgagcggcg gtttgcgaat ccaggtcctg accgcgcatg gtaacctggc
    10561 gaccctgcat tttatcctgt aggtcaaagt atttttcggg tccgagactg ttcatcccca
    10621 ggtgatcgac aaattcgccg aactgccgcg ggttctgttg gtacatctga gcgacgtcct
    10681 gaggattaac gccaacacga gctaactcac cggcgttgtt ttgcagccat gattgcattg
    10741 cttctggaga cgatgacgca aggcgtgcgc cagccgctaa ggtgccgata gaattacgct
    10801 ggtcttcatc aatgaatccc atgcctttac gaacggattc aatctggtct ggatattgag
    10861 tagccaactg acgcaaagca ccgcgatcac cagacgcata agcattagcg tacgcctgct
    10921 gaaattcttt ctgccgctga gcctgctttt cctgctgaaa cacccccgca atacctgaaa
    10981 ggccttgcaa agcagtcagc ccaacattgt tagcgcctga acgctcaata tcattgttct
    11041 gcctgataag ctgaagcgta ttgccgatgt catttacgtt cggagcgttt gagttgacgc
    11101 cgccgatacc agccaataat ccgccatttg atccttgcca agtagccatg attacccctt
    11161 aaaacaacga gccaagcaat ccgataccag caccaatgcc agcgccccaa ggtgttgatg
    11221 ttcccaaaag gctggcaaga cctgcaccgg caatcgcacc agacgttccg ccactaattg
    11281 ctgtctgaag acttgatggt ttgttggcat tagcagcggc aagagctgcg ctttgctgtg
    11341 caatgctgct catgttgttg gcgta
//
LOCUS       DAJVQH010000125        11114 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJVQH010000125 DAJVQH010000000
VERSION     DAJVQH010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.125"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            411..2051
                     /gene="trg"
                     /locus_tag="OQM87_003394"
     CDS             411..2051
                     /gene="trg"
                     /locus_tag="OQM87_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCS1509408.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTVLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2089..3012)
                     /locus_tag="OQM87_003395"
     CDS             complement(2089..3012)
                     /locus_tag="OQM87_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1509409.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGHAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKESLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINTIRKSMPF"
     gene            3229..4572
                     /locus_tag="OQM87_003396"
                     /pseudo
     CDS             3229..4572
                     /locus_tag="OQM87_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4797..6452
                     /gene="opgD"
                     /locus_tag="OQM87_003397"
                     /gene_synonym="mdoD"
     CDS             4797..6452
                     /gene="opgD"
                     /locus_tag="OQM87_003397"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCS1509410.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTLQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6592..6816
                     /locus_tag="OQM87_003398"
     CDS             6592..6816
                     /locus_tag="OQM87_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCS1509411.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6879..7415
                     /gene="rimL"
                     /locus_tag="OQM87_003399"
     CDS             6879..7415
                     /gene="rimL"
                     /locus_tag="OQM87_003399"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCS1509412.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7410..8390)
                     /locus_tag="OQM87_003400"
     CDS             complement(7410..8390)
                     /locus_tag="OQM87_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCS1509413.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8514..9506
                     /gene="tehA"
                     /locus_tag="OQM87_003401"
     CDS             8514..9506
                     /gene="tehA"
                     /locus_tag="OQM87_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCS1509414.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9503..10096
                     /gene="tehB"
                     /locus_tag="OQM87_003402"
     CDS             9503..10096
                     /gene="tehB"
                     /locus_tag="OQM87_003402"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCS1509415.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10399..11067
                     /locus_tag="OQM87_003403"
     CDS             10399..11067
                     /locus_tag="OQM87_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCS1509416.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
BASE COUNT         2868 a         2589 c         2793 g         2864 t
ORIGIN      
        1 aggcgctttt caaaagggta ttttggcttt gacatattag gggctattcc atttcatcgt
       61 ccaacaaaat gggtgcagta cagttgatag aaatatcggt cggggccttc gtctttctga
      121 ttcccggtta gcctgaaaac agaaagtctc aggcacccgc aggcatccta tgaggtttcc
      181 ttagggacga aaataatcac ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat
      241 tcgggttagc cagtttagcc attcgttact ctcttcattc caatagcatt aattttctct
      301 gcaataattg ttgtaaaaat gtgacgcaaa gaggtttttg gtcataagta attaccgtca
      361 agtgccgatg actttctatc aggagtaaac ctggacgaga gacaacggta atgaatacaa
      421 ctccctcaca gcgattaggt tttttgcatc acatcaggct ggttccgtta tttgcctgca
      481 ttcttggcgg tatcttagtt ctattcgcat taagttctgc tctggctggc tatttcctct
      541 ggcaggccga tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg accgtgttag
      601 cgaacagttc agattttttg cgttcagccc ggatcaatat gattcaggcc ggggctgcga
      661 gtcgtattgc ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa tcggagatta
      721 aacagtcgca gcaaggttat cgtgcttatc agaatcgacc ggtgaaaaca cctgctgatg
      781 aagccctcga cactgaatta aatcaacgct ttcaggctta tatcacgggt atgcaaccta
      841 tgttgaaata tgccaaaaat ggcatgtttg aagcgattat caatcatgaa agtgagcaga
      901 tccgaccgct ggataatgct tataccgata ttttgaacaa agccgttaag atacgtagca
      961 ccagagccaa ccaactggcg gaactggccc atcagcgcac ccgcctgggt gggatgttca
     1021 tgattggcgc gtttgtgctt gccctggtca tgacgctgat aacatttatg gtgctacgtc
     1081 ggatcgtcat tcgtccactg caacatgccg cacaacggat tgaaaaaatc gccagtggcg
     1141 atctgacgat gaatgatgaa ccggcgggcc gtaatgaaat cggtcgctta agtcgtcatt
     1201 tacagcaaat acagcattca ctggggatga cagtagggac tgttcgacag ggcgcggaag
     1261 agatttatcg tggcaccagc gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg
     1321 aagaacaagc ggcggctatc gaacaaactg ccgccagcat ggagcaactc actgcgacgg
     1381 tgaaacagaa tgcggataac gcgcatcatg ccagcaaact ggcgcaagag gcttctatta
     1441 aagccagcga tggcgggcag acggtttccg gtgtagtaaa aacgatgggc gctatctcca
     1501 cgagttcgaa gaaaatttct gagatcaccg ccgtcatcaa cagtattgct ttccagacga
     1561 atattctggc actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa gggcgtggat
     1621 ttgccgttgt cgccagcgaa gtacggacac tcgcaagccg cagcgctcag gcagcgaaag
     1681 agattgaagg cttgatcagt gaatcagtca ggttaattga cctggggtcg gatgaggtgg
     1741 caacggcagg gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc acacatatca
     1801 tgcaggaaat cgccgccgcc tcggatgaac aaagtagagg cataactcag gttagccagg
     1861 cgatttctga aatggataag gtgacgcaac agaatgcttc tctggtagaa gaggcctcag
     1921 cagcggcggt gtcccttgaa gaacaggctg cacgattaac tgaggcggtg aacgtattcc
     1981 gtctgaacaa acattctgtg tcggcagaac ctcgcggagc gggtgaacca gttagtttcg
     2041 ctacggtgtg aaaatgttca aggagggatc gacagatccc ttcacctttc agaacggcat
     2101 tgattttcga atagtgttaa tcatcaactg gcaaccagaa gagaacgtcg catctacgcg
     2161 ggtcagtatt ccaatcggtt cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt
     2221 ggcatgacgc aggtcgtctt ttacagcgcc agaagggaca aaccacacgt aatcgtattc
     2281 aacggtaagt tgacgagata gcgaagcaga cagcgtttcg atacaacccg aaggaatttt
     2341 acagccctgg ctctgcacta atgcatctga atgctggcgt ggcgcagtgc cttctggtga
     2401 tacaacgacc ggccattcca gcacccggct tagcgttacg ttctcctgaa gtagcgggtg
     2461 attagggcgg acaaccagct tcaacgattc aagaaacagc agttcgtaat taagcccggt
     2521 catcagttca ggatctgaca tccgaccaat gccgatatcg atttccccgg tttttaaacc
     2581 cgccagaatc atagggttac tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg
     2641 atgaaactga cctataaccg aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac
     2701 cctgacgaca tcattattaa gactttcttt acgatgaagc gactgtccgg cagtgttgat
     2761 ggcgtcaaga actctgactg catgcgttaa aaattgttcg ccgggtaagg taagttgcgc
     2821 cccctgacga ccacgctcaa acaagcgagc gcccgtcagc tgctccagtt cattcaatgt
     2881 attagagagc gcaggttgac tcaaattaag ggtttcagct gcgtgcccca aagttccttg
     2941 ttgtgcgaca gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac taaacagact
     3001 atttttttcc ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg
     3061 attatgataa cttgattgta aaatattatt aacaattaaa gcaattatgt tacagcaaaa
     3121 tggataatat tgatgttttc gcggcgagat cacagtttgt aaattcttcc cgccagagtg
     3181 aatgctgtta cctacactcc agattactga ccactggagg cagacactat ggcgaacagc
     3241 atcacggcgg atgagattcg ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa
     3301 gaagttccgc aatatggcac gctgctggaa ctggtagctg atgtgaatct ggctgtgctg
     3361 gaaaacaatc ctcaactgca cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat
     3421 gttgaacgtc atggggcgat tcgcgttggg actgcacaag agcttgctac tcttcggcgg
     3481 atgtttgcca ttatggggat gtacccggtg agctattacg atctctcgca ggcaggggtg
     3541 ccggtacatt cgacagcatt tcggcccatt gatgatgctt ctctggcgcg taatcccttc
     3601 cgcgttttta catccttact ccgccttgag cttatcgaga acgaaatttt gcgccagaaa
     3661 gcggcggaga ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca actgttagag
     3721 gaatatgagc agcagggcgg ttttaacgaa acacaggcac aggagtttgt gcaggaagcc
     3781 ctggaaacgt ttcgctggca ctagttagca acggtagatg aagaaaccta tcgcgccttg
     3841 cacaacgaac atcggttgat tgctgatgtg gtctgttttc ctggatgcca tatcaaccac
     3901 ctgacgccac gtacgctgga tattgaccgg gtgcagtcga tgatgcctga atgcggaatt
     3961 gaacccaaaa ttctcatcga ggggccgccg cgccgcgagg taccgatttt actacgccag
     4021 accagcttta aagcactgga agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat
     4081 accgcgcgct ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa
     4141 ctgtatgatg atcttctgcg taacgctgga accgggcagg ataatctcac tcaccaaatg
     4201 catttacagg aaaccttccg cacttttcct gacagtgagt ttttaatgcg tcagcaaggg
     4261 ttggcatggt tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat
     4321 cctggagacg atccacagcc cttaattgaa cgtggttggg tagtggcgca acccatcacc
     4381 tatgaagatt tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa
     4441 acgcagacac gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc gttgggttgt
     4501 ccggttttgg atgagttcca gctttaccaa gaagcggaag aacgcagtaa acgtcgctgt
     4561 ggtttgcttt aaattctgac catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa
     4621 tcaggctcat cccatcgaca taaaaaaaat gccgatttat gcatattctc tcagttcaac
     4681 aattggatta ttaataaata ttgtctagag tgagcggtca taaataagca ctttcttgcc
     4741 gctgaaaacg accagcgcgg gaccattcac aacaccagaa ggactcactt tcaggtatgg
     4801 atcgtagacg atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt accagcggca
     4861 ttgcttctct tttttctcag gcagcattcg cggcagattc tgatattgcc gacgggcaaa
     4921 cccagcgttt tgacttctcc attctacagt caatggcgca cgacttagcg caaacagcgt
     4981 ggcgtggtgc gcctcgtccg ttacctgaca cgctggcgac aatgacgctg caggcttata
     5041 acagtattca atacgacgcc gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg
     5101 acgctcagtt cttccatatg ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag
     5161 atccagcaac acatctggcg cgtgaaattc actttcgccc ggagttgttc aaatacaacg
     5221 atgcaggtgt tgataccaaa caattagaag ggcaaagcga tctcggcttt gccggttttc
     5281 gcgtgtttaa agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt
     5341 atttccgcgc cgttgatgat acatatcaat acggtttgtc ggcccgcggc ctggcgatcg
     5401 acacttacac cgacagtaaa gaagagttcc ccgactttac cgccttctgg tttgatacgg
     5461 taaaaccggg ggcaactacc tttaccgttt atgcgttgct cgatagcgcc agcattactg
     5521 gtgcctataa gttcactatc cattgtgaga aaagtcaggt gattatggat gtggaaaatc
     5581 acctgtatgc gcgcaaagac attaaacagc tgggcatttc gccgatgacc agtatgttca
     5641 gctgcggtac taatgaacgt cggatgtgcg atacaattca tccgcaaatt catgactctg
     5701 atcgtctgtc catgtggcgg ggcaacggcg agtggatttg ccgtccgctg aataatccgc
     5761 aaaaattgca gttcaatgct tacaccgaca acaacccgaa agggtttggt ttattgcaac
     5821 tggatcgtga cttctcccat tatcaggaca ttatgggctg gtataacaaa cgcccaagtc
     5881 tgtgggtgga accgcgtaac aagtggggta agggcaccat cggcctgatg gaaatcccaa
     5941 caacgggcga aacgctggat aacattgtct gcttctggca gccagaaaaa gctgtaaaag
     6001 caggtgatga gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt
     6061 gcccattagc gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg gaaggttggg
     6121 cgccaggtga acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg
     6181 gtgatctgaa agctgccgcg ccaaaaggca ttgagccggt gattacgctt tccagtgggg
     6241 aagcgaagca aatcgaaatt ctctatattg aacccatcga tggttatcgt attcagtttg
     6301 actggtatcc gacttcggac tccactgatc cggtcgatat gcggatgtat ctacgttgtc
     6361 agggggacgc tatcagtgaa acatggctgt atcagtattt cccgccagcg ccggataaac
     6421 gtcagtatgt tgacgaccgc gtgatgagtt aatcgttttt tcttcggcac cttcttcggg
     6481 aggtgccgtc tggttaaaca cgatcccgct cgcatttttc cctaagttaa atgagtaatc
     6541 tgatggtgtg tatttcagat acaccgtgtc agccactaac agggagtgcg tatgtttcca
     6601 gaataccgag atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc
     6661 gataaacaca ataaacttga tcatgaaatt gccagaaagg aaggttccga cggtcgaggg
     6721 tacaatgcgg aagtggtccg catgaaaaaa caaaagctac agttaaaaga tgagatgctc
     6781 aaaatcctgc agcaggagag cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc
     6841 ggctttttta acaacagcat ggattgactg gagataagat gactgaaacg ataaaagtaa
     6901 gcgaatcact tgaattacat gctgttgcgg aaagtcacgt cacacctctt tatcagttaa
     6961 tctgtaaaaa taaaacctgg ttacagcagt cgctaaactg gccgcagttc gttcagagtg
     7021 aagaggacac gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca
     7081 aaatgttcat gattttcaaa gaagatgaac ttatcggcgt tatctcgttt aatcgtattg
     7141 aaccactgaa taaaactgct gaaatcggct actggctgga cgaatctcat caggggcagg
     7201 ggatcatttc tcaggcgctg caggcattga ttcatcatta cgctcagtct ggtgaactta
     7261 gacgcttcgt gatcaaatgt cgggtggaca atccgcaaag caaccaggtc gctttgcgca
     7321 atggttttat ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat gcctatgatg
     7381 atgtgaactt atacgcgcgt attatcgatt cataataacc ctgccagcgg cgttcgcgta
     7441 atgcgctcct cgccgttaat gacttttgga ccacggacat gaaccgtgtc gccatcaaag
     7501 gcttcaacga cggcgtggtc ggttaactcc acatggtttt caattatcac cgcacctgta
     7561 atacggctct caccttgtat tacaacgtgc tcgtcgagca gaatcggtcc accacgtacc
     7621 acggcatttc cgccaatcaa gacatgatgt tttaacacac aattaccttc cacaatggca
     7681 tattccgcca cctgcgaact gtaatgaatc gtcggaatgg catcttcttc tatgccagct
     7741 ttcacctgcg cgtgaccgta gactttagcg caatcgcata gccagacgtt gttctcttca
     7801 ttaccttcaa cactcgcaaa atcaaaaact tcggcgcgat gttcaataaa agcataccgg
     7861 acgacggcat cgccataaat ttgtgcctgg tggacaatac gcgaggcgct aacccttgcg
     7921 cgatcataaa tttggagcag gagttgatgg tcgggcgtta agccttgtgc ggcgacgatc
     7981 atagaatgtt gatcaattaa ggcgtgacca aatattctac attgcccgta aactaatgag
     8041 tcatgaatgg tgacactgtc acttatatat gcaccctggc tgatttcact attatcgatc
     8101 cagacgttat ccgttgcgta aacctctccc cacagcacgc tggtgccggt gatacgcgta
     8161 ttaccggaaa tcaccgttcc gccgaacgca atagcattct ggtcgtaaat ccagcaattt
     8221 ccttcctgtg ctaacaccgt ttcgcgatcg atccagccgc cagcggttcc tgctatcaca
     8281 tcgttaaaat cgctaatggc gataatctgc cgtaataaca cacttttttt agtgccatct
     8341 tcctgataac taaaggctcg ctgttcttca cttaggcgat atttgcgcat agcgttttcc
     8401 cacaggtgac ttactataac cgtagcaaat tctgcggctc tggctatgct cgagaaaatt
     8461 ccataaaatg catttcaaat atactttata aattaaacaa aatgagtaag aagatgcaga
     8521 gcgataaagt gctcaatttg ccggcaggct actttggtat tgtgttgggg acgataggga
     8581 tgggatttgc ctggcgctat gccagccagg tttggcaggt cagccactgg ttaggggatg
     8641 ggctggtgat tctggcgatg atcatctggg gattattgac tagcgcattt attacccgac
     8701 tcatacgctt tccgcatagc gtgctggcgg aagttcgcca tccagtgctg agcagttttg
     8761 tgagtttgtt tccggcaacg acgatgctgg tggcgattgg ttttgttccg tggtttcgcc
     8821 cactggcggt gtgcatgttc agttttggtg tcgtggttca gttggcttat gccgcctggc
     8881 aaactgcggg attatggcgc ggatctcacc ctgaggaagc taccacgcct ggactgtatc
     8941 tgccgacagt tgccaacaac tttatcagcg caatggcctg tggtgcgttg ggctacaccg
     9001 acgccggtct ggtgttttta ggcgcaggcg ttttctcatg gctaagcctt gaaccggtga
     9061 tcttgcagcg tctgcgcagt tcgggagaat tacccacggc actgcggaca tcactcggca
     9121 ttcagctcgc gcctgcgctg gtggcctgta gtgcctggct gagcgtcaac ggcggcgagg
     9181 gtgacacgct ggcgaaaatg cttttcggtt atggactgct gcaactgctg tttatgctac
     9241 gtctgatgcc atggtatctc tcccagccat ttaatgcttc attctggagt ttctcgttcg
     9301 gcgtatctgc actggcaacc accggtttgc atctggggag tggcagcgat aatggatttt
     9361 tccatacgct ggcggtgccg ctgtttatct ttaccaattt tattattgca atactgctca
     9421 tccgtacttt tgcgcttctg atgcagggaa aattgttagt cagaaccgag cgcgccgttt
     9481 taatgaaagc agaggacaaa gaatgatcat tcgtgacgaa aactatttta ctgataaata
     9541 tgaattaacc cgcacacact ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa
     9601 aacgctggat ctgggctgtg gcaatggtcg taacagtctt tacctggcag ccaatggtta
     9661 tgatgttgac gcatgggata aaaatgccat gagtatcgcc aacgtcgagc gcattaaatc
     9721 cattgaaaat ctggataatt tacacacccg ggtcgttgat ctgaataacc tcacatttga
     9781 tggacagtac gattttattc tttcgactgt ggtgctgatg ttccttgagg ctaaaaccat
     9841 cccagggttg attgccaata tgcaacgttg cactaaacct ggtggttaca acctgattgt
     9901 ggcggcgatg gataccgctg attatccatg taccgtcggc ttcacgtttg ccttcaaaga
     9961 gggagaatta cgtcgatatt acgaaggctg ggagagggtg aaatacaatg aagacgtcgg
    10021 cgagctgcac cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct
    10081 ggcacgtaaa aaatgacccg gtaagcacaa aacgcgtgaa aattccccac gctgagatga
    10141 tttactgttc ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa attaatcaca
    10201 gtagtcgaag ttttttagca gcttaactta ctgaaattta actactgatg attgacttag
    10261 cccctttttc ggcattgact atgtcgtctg aaaaggggct gaaaaattta ttttcaccaa
    10321 cacttttttt gccacaacac gaagcggcgc tttttgctat aacttagaaa gtaatataat
    10381 catctcagga aactattcat gcgtaccaca tcatttgcga aagttgcagc tttatgcggc
    10441 ttgttggctc tgtctggttg tgcctctaaa atcacccagc cagataaata ttctggtttt
    10501 ttaaacaatt actctgattt aaaagaaaca acctcagcaa caggtaaacc tgttttacgt
    10561 tgggtagacc cgagttttga tcaaagcaaa tatgacagca tcgtctggaa cccaatcact
    10621 tattatccgg taccaaaacc ttctacccag gtagggcaga aagttctgga taaaattttg
    10681 aactatacca acaccgaaat gaaagaagcg atagcgcagc gtaaaccagt ggttaccacc
    10741 gctgggccgc gtagtctgat tttccgtggg gccattaccg gtgtagatac cagcaaagaa
    10801 gggctgcaat tctatgaagt ggttcctgtt gcattagtgg ttgcggggac gcaaatggct
    10861 acaggccacc gtaccatgga tactcgcctc tattttgaag gtgagctgat tgatgcagcg
    10921 actaataaac cggttatcaa agtcgttcgt cagggcgaag gtaaagacct gaataacgaa
    10981 agtacgccaa tggctttcga aaatattaaa caagttattg atgacatggc gaccgatgcc
    11041 accatgtttg acgttaacaa aaagtagttc cagacgcgcc atcgttagat ggcgcttttt
    11101 atccggtgcg ccgt
//
LOCUS       DAJVQH010000126        10953 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJVQH010000126 DAJVQH010000000
VERSION     DAJVQH010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.126"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            34..393
                     /locus_tag="OQM87_003404"
     CDS             34..393
                     /locus_tag="OQM87_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCS1509417.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(513..692)
                     /locus_tag="OQM87_003405"
     CDS             complement(513..692)
                     /locus_tag="OQM87_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCS1509418.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            766..2127
                     /gene="pabB"
                     /locus_tag="OQM87_003406"
     CDS             766..2127
                     /gene="pabB"
                     /locus_tag="OQM87_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCS1509419.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQKVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2131..2709
                     /locus_tag="OQM87_003407"
     CDS             2131..2709
                     /locus_tag="OQM87_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCS1509420.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2893..4257
                     /gene="sdaA"
                     /locus_tag="OQM87_003408"
     CDS             2893..4257
                     /gene="sdaA"
                     /locus_tag="OQM87_003408"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1509421.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDNLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4388..5986
                     /locus_tag="OQM87_003409"
     CDS             4388..5986
                     /locus_tag="OQM87_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1509422.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(5990..7540)
                     /gene="yoaE"
                     /locus_tag="OQM87_003410"
     CDS             complement(5990..7540)
                     /gene="yoaE"
                     /locus_tag="OQM87_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCS1509423.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENCDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            8003..8974
                     /gene="manX"
                     /locus_tag="OQM87_003411"
     CDS             8003..8974
                     /gene="manX"
                     /locus_tag="OQM87_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCS1509424.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFIPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9037..9837
                     /gene="manY"
                     /locus_tag="OQM87_003412"
     CDS             9037..9837
                     /gene="manY"
                     /locus_tag="OQM87_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCS1509425.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9850..10701
                     /gene="manZ"
                     /locus_tag="OQM87_003413"
     CDS             9850..10701
                     /gene="manZ"
                     /locus_tag="OQM87_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCS1509426.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10756..>10953
                     /locus_tag="OQM87_003414"
     CDS             10756..>10953
                     /locus_tag="OQM87_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HCS1509427.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHG"
BASE COUNT         2679 a         2784 c         2771 g         2719 t
ORIGIN      
        1 cgtgccatat cgttataatc cctttgatat tagatgcaaa ttaaggtcat atatagcctt
       61 atcgataaca tggttaattt taaggataag aatatgcctg ctgtaataga taaagccctg
      121 gatttcattg gtgccatgga tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcacg
      181 gcgaagggaa tctttaaata tttaaaagaa ctgggagtac ccgcgagtgc cgctgatatt
      241 acggcgcgag ctgaccagga aggctggaac cctgggttca cggaaaaaat ggttggatgg
      301 gcaaaaaaaa tggagacagg tgaacgctct gtgattaaaa atcctgaata cttttcaaca
      361 tatatgcagg aagaactaaa agcactggtc tgagttaaat ttatatcagc ataaatgggt
      421 caggacgctt ttaatcacat ataaaaagcg tcctttttct cacaatcaaa tgatcatcgg
      481 cttctataac gaaatcgata cggattagtg tgttactcgt cttcatcctc aaaacgcgcc
      541 acgattagct cgccggagtg gttggcacgc agctcttctg ccaccagcgc gattgcctgg
      601 ccgctactca tcccttgtgc catcagttcc tggatccgct cgacagcttt ttgctgctgt
      661 tcatgggtga gtgaaggtaa acctgcaaac attgttaact cctgctaaat tgttggcgct
      721 aattatttca tgctacccgg cacatagcca gtagagtcag gactgatgaa gacgttatct
      781 cccgctgtga ttactttacc ctggcgtcag gacgccgctg aattttattt ctcccgctta
      841 agccacctgc cgtgggcgat gcttttacac tccggctatg ccgatcatcc gtatagccgc
      901 tttgatattg tggtcgccga tccgatttgc actttaacca ctttaggtaa agaaaccgtt
      961 gttagtgaaa gcgaaaaacg cacaacgacc actgatgacc cgctacaggt gctccagaag
     1021 gtgctggatc gcgcagacat tcgcccaacg cataacgaag atttgccatt tcagggcggc
     1081 gcactggggt tgtttggcta cgatctgggc cgccgttttg agtcactgcc agaaattgcg
     1141 gaacaagata tcgttctgcc ggatatggca gtgggtatct acgattgggc gctcattgtc
     1201 gaccaccagc gtcatacagt ttctttgctg agtcataatg atgtcaatgc ccgtcgggcc
     1261 tggctggaaa gccagcaatt ctcgccgcag gaagatttca cgctcacttc cgactggcaa
     1321 tccaatatga cccacgagca gtacggcgaa aaatttcgcc aggtacagga atatctgcac
     1381 agcggtgatt gctatcaggt gaatctcgcc cagcgttttc atgcgaccta ttctggcgat
     1441 gaatggcagg cattccttca gcttaatcag gccaaccgcg cgccatttag cgctttttta
     1501 cgtcttgaac agggtgcaat tttaagcctt tcgccagagc ggtttattct ttgtgataat
     1561 agtgaaatcc agacccgccc gattaaaggc acgctaccac gcctgcccga tcctcaggaa
     1621 gatagcaaac aagcagaaaa actggcgaac tcagcgaaag atcgtgccga aaatctgatg
     1681 attgtcgatt taatgcgtaa tgatatcggt cgtgttgccg tagccggttc ggtaaaagta
     1741 ccagagctct tcgtggtgga acccttccct gccgtgcatc atctggtcag cactataacg
     1801 gcgcgactac cagaacagtt acacgccagc gatctgctgc gcgcagcttt tcctggtggc
     1861 tcaataaccg gggctccgaa agtacgggct atggaaatta tcgacgaact ggaaccgcag
     1921 cgacgtaatg cctggtgcgg cagcattggc tatttgagct tttgcggcaa catggatacc
     1981 agcattacta tccgcacgct gactgccatt aacggacaaa tatactgctc tgcgggcggt
     2041 ggaattgtcg ccgatagcca ggaagaagcg gaatatcagg aaacttttga taaagttaat
     2101 aagatattac gccaactgga gaagtaaaac gtggaatacc gtagcctgac gcttgatgat
     2161 tttttatctc gctttcaact tttgcgcccg caaattaacc gggaaaccct aaatcatcgt
     2221 caggctgccg tgttaatccc catcgtccgt cgaccacaac cggggttgtt gctgactcag
     2281 cgttcaattc atctgcgtaa acacgctgga caagtggcat tccctggagg tgcagtcgat
     2341 gacacggacg catcagttat cgccgccgcg ctgcgcgaag ctgaagaaga ggtcgctata
     2401 ccgccttccg ccgttgaagt tatcggcgtg ctgccgcccg tcgatagcgt aactggctat
     2461 caggtaaccc cagtggtcgg cattatcccg cccgatctgc cgtatcgcgc cagtgaagat
     2521 gaagtctcgg cggtgtttga aatgccgctc gcccaggcat tacatctggg tcgttatcac
     2581 cctttagata tctaccgccg tggcgattca catcgggtat ggctgtcctg gtacgaacag
     2641 tattttgtat ggggaatgac cgcaggcata attcgtgagc tggcgctgca aattggtgtg
     2701 aacccctgac tatacttatc tttacatcta caaaacacta cttgagacaa tcatcgcaat
     2761 attagttaaa tcgcggtttt tgattagttt aattcatgtg aatagttaag ccagtcgccg
     2821 cgttccctct tacactatgc gctgttatta gttcgttact ggaagtccag tcaccttgtc
     2881 aggagtatta tcgtgattag tctattcgac atgtttaagg tggggattgg tccctcatct
     2941 tcccataccg tagggcctat gaaggcgggt aaacagttcg tcgataatct ggtcgaaaaa
     3001 ggcttactgg atagcgttac tcgcgttgcc gtggacgttt atggttcact gtcgctgacg
     3061 ggtaaaggcc accacaccga tatcgccatt attatgggtc ttgcaggtaa cgaacctgcc
     3121 accgtggata tcgacagtat tcccggtttt attcgcgacg taaaagagcg cgaacgtctg
     3181 ctgctggcac agggacggca tgaagtggat ttcccgcgcg acaacgggat gcgttttcat
     3241 aacggcaacc tgccgctgca tgaaaacggt atgcaaatcc acgcctataa cggcgatgaa
     3301 gtcgtctaca gcaaaactta ttattccatc ggcggcggtt ttatcgtcga tgaagaacac
     3361 tttggtcagg atgctgccaa cgaagtgagc gtgccgtatc cgttcaaatc tgccaccgaa
     3421 ctgctcgcgt actgtaatga aaccggctat tcgctgtctg gtctcgctat gcagaacgaa
     3481 ctggcgctgc acagcaagaa agagatcgac gagtatttcg cgcatgtctg gcaaaccatg
     3541 caggcatgta tcgatcgcgg gatgaacacc gaaggtgtac tgccaggccc gctgcgcgtg
     3601 ccacgtcgtg cgtctgccct gcgccggatg ttagtttcca gcgataaact gtctaacgat
     3661 ccgatgaatg tcattgactg ggtaaacatg tttgcgctgg cagttaacga agaaaacgcc
     3721 gccggtggtc gtgtggtaac tgcgccaacc aacggtgcct gcggtatcgt tccggcagtg
     3781 ctggcttact atgaccactt tattgaatcg gtcagcccag acatctatac ccgttacttt
     3841 atggcagcgg gcgcgattgg tgcattgtat aaaatgaacg cctctatttc cggtgcggaa
     3901 gttggttgcc agggcgaagt gggtgttgcc tgttcaatgg ctgctgcggg tcttgcagaa
     3961 ctgctgggcg gtagcccgga acaggtttgc gtggcggcgg aaattggcat ggaacacaac
     4021 cttggtttaa cctgcgaccc ggttgcaggg caggttcagg tgccgtgcat tgagcgtaat
     4081 gccattgcct ctgtgaaggc gattaacgcc gcacggatgg ctctgcgccg caccagtgca
     4141 ccgcgcgtct cgctggataa ggtcatcgaa acgatgtacg aaaccggtaa ggacatgaac
     4201 gccaaatacc gcgaaacctc acgcggtggt ctggcaatca aagtccagtg tgactaatac
     4261 ttcttactcg cccatctgca acggatgggc gaatttatac ccgctttctc gtctgctgta
     4321 atattcccca ctacacttcc actgttgcgt caggcgtttg tcgccatacg cttacagggt
     4381 ggcccgcatg caaaaagcac agcggatcat taaaacctat cgccgtaatc gaatgattgt
     4441 ttgtacgatt tgcgcactcg ttacgctcgc ttcgaccctg agcgtgcgat ttatttcaca
     4501 gcgtaactta aatcaacaac gggtagtaca attcgccaat cacgctgtag aggaattaga
     4561 taaagtactg cttcccctac aggcaggtag cgaagtcttg cttccgctga ttggtctgcc
     4621 ctgctctgtc gcccatttgc cattacgtaa acaggcggca aaactccaaa ctgtgcgatc
     4681 cattggcctg gtgcaagacg gcacacttta ttgctccagc atttttggtt atcgcaatgt
     4741 gcccgtcgtg gacattctgg ctgaacttcc tgcaccgcaa ccacttttac gcctgacgat
     4801 cgaccgtgcc ctgattaaag gcagtccggt tttgattcaa tggacgcctg cagcaggcag
     4861 tagcaatgct ggggtcatag agatgattaa catcgactta ctgacggcaa tgctccttga
     4921 gccacaactg ccgcaaatca gtagcgccag cctgacggtg gacaaacggc atttgctcta
     4981 tggtaatggg ctggtagatt cccttccgca acctgaagac aatgaaaact atcaggtttc
     5041 ttcgcaaagc tttcctttta ccattaacgt aaatggtccg ggggctacgg cgctggcatg
     5101 gcactatctt ccaacacaat taccgctggc ggtgctgcta agtttactgg tgggctatat
     5161 cgcctggctg gcgaccgctt accggatgag cttttcccgc gaaattaatc tgggcctggc
     5221 gcaacatgag ttcgaattgt tctgtcagcc tttgcttaat gcgcgcagcc agcaatgtat
     5281 tggtgtagag attttgctgc gctggaataa tccgcgtcag ggctggattt caccggatgt
     5341 gtttattcct atcgcggaag aacatcattt aattgtgcca ctgacccgct atgtgatggc
     5401 agaaaccatt cgtcagcgcc atgttttccc gatgagtagt cagtttcatg ttggcattaa
     5461 cgtcgcaccc agccattttc gccgtggtgt gctgataaaa gatctcaatc agtactggtt
     5521 tagcgctcac ccgattcagc aactgatcct cgaaatcacc gaacgcgatg ccttactgga
     5581 tgttgattat cggattgccc gcgagctgca tcgtaaaaac gtcaaactgg cgattgatga
     5641 cttcggcacc ggcaacagct cgttttcctg gcttgaaaca ttacgtcctg acgtgctgaa
     5701 aattgataag tcatttaccg cagctatagg ttctgacgcg gttaactcga cggtgaccga
     5761 tatcatcatc gcgctggggc aaagactgaa tattgaactg gtggcggagg gcgtggaaac
     5821 gcaagaacag gcgaagtatt tgcgccgtca tggcgtgcat attttgcaag ggtatttgta
     5881 cgcacagccg atgccgctac gtgattttcc caaatggctg gcgggcagcc aaccgccgcc
     5941 cgcccggcat aatggacata tcacgcccat tatgtcgtta cgttaacgct tactcatctt
     6001 catcgtgcgc cggttgctct ttaacaatgc gaaccagatc aacacgataa tcattggctt
     6061 caaggatggt gatatgcagt ggccctacat caatcacatc gcccacacgg ggaatgtgac
     6121 catttgccga gatcacgagg cccgcgaccg tcgcgatatc atcgtcatcg gcaaggtgct
     6181 caacatcaag cgcctgctgc aaggcatgca aatctgtacc gccttttacc agccagccgt
     6241 taccatcggt aatgatttcc ggcgtttcgt cagcgtccgg gaattcaccc gcaatggctt
     6301 ccagcacatc cagcggcgtg accaagcctt gtaccacacc aaactcgttg gtcacgataa
     6361 caaagctccc gcgagcgcga cgcagcacgc ccagcaggtt gatcggatcg agggtttccg
     6421 ggacgataat cgccggagac gccgaagcaa tcgccgccac atcaacgccc tcttccagcg
     6481 ccaccagcag ttctttagca cgtacgatac cgatgatttc atccagttca ccgcgacata
     6541 ccgggaacag actgtgcggt gaagagagca gttgctcgcg gatttcatcg accccgagat
     6601 tagcgtcaac ccagcttatt tcaccgcgcg gcgtcatgat cccgcgcaga gaacgcgacg
     6661 ccagcgtcag tacgccgtta atcatgtacc gttcttcttc ggcaaatgca ccttccggga
     6721 tcggcatcgg gttatcggca tcgtgctgaa cattggcctg acgtttcccg cccatcaaac
     6781 gcaggatggc atcggcagta cgcgctcgca gcggcaaagt cgactggtga cgaataaagt
     6841 tgcgacgcgc aatctggtta aacacttcga taatgatcga gaagccaatc gcggcataca
     6901 ggtaaccttt cggaatgtgg aaaccgaaac cttctgccac cagactcaga ccaatcatta
     6961 acaggaagct cagacagagc accaccaccg tggggtgctg gttaacgaat cgcgtcagcg
     7021 gtttcgatgc cagcaacata accgccatcg caatcactac cgccgccatc atcaccggca
     7081 gatggttaac catccctact gcagtaatta ccgcatccaa cgagaagacg gcgtcaagga
     7141 tgacaatctg tgtgacgacc acccagaaac tggcgtagcc tttaccgtgg ccggaatcat
     7201 gatcgcagtt ttccagccgt tcatgcagtt cggttgttgc tttgaacagc aagaatatcc
     7261 ccccgaacaa cataatcaga tcgcgtccgg agaaggagaa atccatgacg gtaaatagcg
     7321 gtttggtcag cgtgaccatc catgaaatca gcgacagcag ccccagacgc ataatcagcg
     7381 ccagtgataa ccccagcaaa cgcgctttat cgcgttgttt tggcggcagt ttgtcagcaa
     7441 gaatggcgat gaagaccagg ttatcgatac ccagcacaat ttcgagaaca acaagcgtga
     7501 gtagccccgc ccaaattgag gggtccatta agaattccat gacaagctcc tgcttaagga
     7561 atagctattc gacgccagaa ataatgcagg cgtaacgaca aaatgcaaac gaaaggtgcg
     7621 gcatagagtg ccagaaaggc aggcgttaaa aggcctgatg ctgaaatgac gtcggtgacg
     7681 atccatactg cgggctactg ccctatactc catggttgtt aaacgggagt taaacatatc
     7741 agagacgcct ctgatttggc aaagatttac cttcctttgc aaacgaatgt gacaaggata
     7801 ttttaccttt cgaaatttct gataatcgaa agttaaatta cggatcttca tcacataaaa
     7861 taattttttc gatatctaaa ataaatcgcg aaacgcaggg gtttttggtt gtagccctta
     7921 tctgaatcga ttcgattgtg gacgacgatt caaaaataca tctggcacgt tgaggtgtta
     7981 acgataataa aggaggtagc aagtgaccat tgctattgtt ataggcacac atggttgggc
     8041 tgcagagcag ttgcttaaaa cggcagaaat gctgttaggc gagcaggaaa acgtcggctg
     8101 gatcgatttc attccaggtg aaaatgccga aacgctgatt gaaaaataca acgctcagtt
     8161 ggcaaaactc gacaccacta aaggcgtgct gtttctcgtt gatacatggg gaggcagccc
     8221 gttcaatgct gccagccgca ttgtcgtcga caaagagcat tatgaagtca ttgcaggcgt
     8281 taacattcca atgctcgtgg aaacgttaat ggcccgtgat gatgacccga gctttgatga
     8341 actggttgcg ctggcagtag aaacaggccg tgaaggcgtg aaagcactga aagccaaacc
     8401 ggttgaaaaa gccgcgccag cacccgctgc cgcagcacca aaagcggctc caactccggc
     8461 aaaaccaatg gggccaaacg actacatggt tattggcctt gcgcgtatcg acgaccgtct
     8521 gattcacggt caggtcgcca cccgctggac caaagaaacc aatgtctccc gtattattgt
     8581 tgttagtgat gaagtggctg cggataccgt tcgtaagaca ctgctcaccc aggttgcacc
     8641 tccgggcgta acagcacacg tagttgatgt tgccaaaatg attcgcgtct acaacaaccc
     8701 gaaatatgct ggcgaacgcg taatgctgtt atttaccaac ccaacagatg tagagcgtct
     8761 cgttgaaggc ggcgtgaaaa tcacctctgt taacgtcggt ggtatggcat tccgtcaggg
     8821 taaaacccag gtgaataacg cggtttcggt tgatgaaaaa gatatcgagg cgttcaaaaa
     8881 actgaatgca cgcggtattg agctggaagt ccgtaaggtt tccaccgatc cgaaactgaa
     8941 aatgatggat ctgatcagca aaatcgataa gtaacgtatt gtgttgatta tcattcagtt
     9001 ttcacactta agtcttacgt aaacaggaga agtacaatgg agattaccac tcttcaaatt
     9061 gtgctggtat ttatcgtagc ctgtatcgca ggtatgggat caatcctcga tgaatttcag
     9121 tttcaccgtc cgctaatcgc gtgtaccctg gtgggtatcg ttcttgggga tatgaaaacc
     9181 ggtattatta tcggtggtac gctggaaatg atcgcgctgg gctggatgaa catcggtgct
     9241 gcagttgcgc ctgacgccgc tctggcttct atcatttcta ccattctggt tatcgcaggt
     9301 catcagagca ttggtgcagg tatcgcactg gcaatccctc tggccgctgc gggccaggta
     9361 ctgaccatca tcgttcgtac tattaccgtt gctttccagc acgctgcgga taaggctgct
     9421 gataacggca acctgacagc gatttcctgg atccacgttt cttctctgtt cctgcaagca
     9481 atgcgtgtgg ctattccggc cgtcatcgtt gcgctgtctg ttggtaccag cgaagtacag
     9541 aacatgctga atgcgattcc ggaagtggtg accaatggtc tgaatatcgc cgggggcatg
     9601 atcgtggtgg ttggttatgc gatggttatc aacatgatgc gtgctggcta cctgatgccg
     9661 ttcttctacc tcggcttcgt aaccgcagca ttcaccaact ttaacctggt tgctctgggt
     9721 gtgattggta ctgttatggc agtgctctac atccaactta gcccgaaata caaccgcgta
     9781 gccggtgcgc ctgctcaggc agctggtaac aacgatctcg ataacgaact ggactaacag
     9841 gtgagcgaaa tggttgatac aactcaaact accaccgaga aaaaactcac tcaaagtgat
     9901 attcgtggcg tattcctgcg ttctaacctc ttccagggtt catggaactt cgaacgtatg
     9961 caggcactgg gtttctgctt ctctatggta ccggcaattc gtcgcctcta ccctgagaac
    10021 aacgaagctc gtaaacaagc tattcgccgt cacctggagt tctttaacac ccagccgttc
    10081 gtggctgcac cgattctcgg cgtaaccctg gcgctggaag aacagcgtgc taatggcgca
    10141 gagatcgatg acggtgctat caacggtatc aaagtcggtt tgatggggcc gctggctggt
    10201 gtaggcgacc cgatcttctg gggaaccgta cgtccggtat ttgcagcact gggtgccggt
    10261 atcgcgatga gcggcagcct gttaggtccg ctgctgttct tcatcctgtt taacctggtg
    10321 cgtctggcaa cccgttacta cggcgtagct tatggttact ccaaaggtat cgatatcgtt
    10381 aaagatatgg gtggtggctt cctgcaaaaa ctgacggaag gggcgtctat cctcggcctg
    10441 tttgtcatgg gggcattggt taacaagtgg acacatgtca acatcccgct ggtggtttcg
    10501 accatcaccg ggcaggatgg acagacccgt gtcaccaccg tgcaaactat tcttgaccag
    10561 ttaatgccag gccttgtacc actgctgctg acatttgctt gtatgtggct gctgcgcaaa
    10621 aaagttaacc cgctgtggat catcgttggc ttcttcgtca tcggtatcgc tggttacgct
    10681 tgcggcctgc tgggactgta agactgttgt acactaccgg ggccttttgg ccccgttttt
    10741 ttatctggag gattaatgac aatcacggac ctggtactga ttcttttcat cgccgcactc
    10801 ctggccttcg cgatctacga tcagttcatc atgccccgcc gtaacggccc caccctgctg
    10861 gcaattcctt tgctccgccg tggtcgcatc gatagcgtta tcttcgtcgg attgattgtc
    10921 attcttatct ataacaacgt cacgaatcat ggc
//
LOCUS       DAJVQH010000127        10879 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJVQH010000127 DAJVQH010000000
VERSION     DAJVQH010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.127"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="OQM87_003415"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="OQM87_003415"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1509428.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="OQM87_003416"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="OQM87_003416"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCS1509429.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="OQM87_003417"
     CDS             3115..3399
                     /locus_tag="OQM87_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1509430.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OQM87_003418"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="OQM87_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCS1509431.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OQM87_003419"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="OQM87_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCS1509432.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OQM87_003420"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="OQM87_003420"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1509433.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="OQM87_003421"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="OQM87_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCS1509434.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9589..10689
                     /gene="ompC"
                     /locus_tag="OQM87_003422"
     CDS             9589..10689
                     /gene="ompC"
                     /locus_tag="OQM87_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1509435.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2674 a         2788 c         2689 g         2728 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacacttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc accgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atgggtgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ttataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaacggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaacaccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcacctcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc atacctcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatgc tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcgctaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9421 atctcatttc gacttcgatc acatatcgat aaataatatt atttcatcta tttttctcat
     9481 tgtttttaat caccattcta ttttaaaaac agtatgtcat ttataaatga cgccgtttaa
     9541 aattcgttta gaaaatacgt ttaacgataa ttaaaaggat tctgttaaat gaaattaaaa
     9601 atagttgcgg tggttgtaac tggtttgtta gctgcgaacg tagcacacgc tgccgaagtc
     9661 tataacaagg atggtaataa actcgacctt tatggcaagg ttaccgctct acgttatttt
     9721 actgatgata agcgtgacga tggtgataaa acttatgccc gtctcggctt taaaggagaa
     9781 acgcaaatca atgatcaaat gattggtttt ggtcactggg aatatgattt taaaggctat
     9841 aacgatgaag ccaacggctc gcgcggcaac aagacccgtc tggcctatgc tggtttaaaa
     9901 attagtgaat ttggctctct ggactatggt cgtaactacg gtgtcggcta cgacattggt
     9961 tcatggaccg atatgttgcc agaatttggt ggcgatacct ggagtcagaa agatgtcttc
    10021 atgacatacc gtaccaccgg tctagcaacc tatcgcaact acgatttctt tggcttaatt
    10081 gaagggctga actttgccgc gcaatatcaa ggcaaaaatg aacgtactga caacagtcat
    10141 ctttatggtg ctgactacac gcgtgccaac ggtgacggtt tcggtatctc ctcaacttat
    10201 gtttatgatg gctttggtat cggtgcggta tataccaaat ccgatcggac aaatgcgcag
    10261 gaaagagccg ctgctaatcc tctcaatgcc tccggtaaga atgcagaact atgggccaca
    10321 ggtataaagt atgatgccaa caacatctac tttgcagcta attacgctga aacattaaac
    10381 atgaccacct atggggatgg ttatatctct aacaaagcac aaagttttga agtggtggcg
    10441 caatatcaat tcgacttcgg cttgcgccca tcactcgctt acctgaaatc gaaaggcaga
    10501 gatctgggcc gctacgccga ccaggacatg attgagtata tcgacgttgg tgcgacgtat
    10561 ttcttcaaca aaaatatgtc gacctatgtt gattataaaa tcaacctgat tgatgaaagc
    10621 gactttaccc gtgccgtaga tattcgcacc gataacatcg tcgctacggg cattacctat
    10681 cagttctaac gtcctctcct gaccagccct tctctgggct ggtttttgat ttaagtgctg
    10741 ctgtatgact ccttaagtaa aaatgagcaa tttttgatat ttctggtcat tcacctcaat
    10801 taatattatt ccatatcctc cggggacatc ttactataat atttattgat gagtgattat
    10861 gaccaatatt aatacagct
//
LOCUS       DAJVQH010000128        10806 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJVQH010000128 DAJVQH010000000
VERSION     DAJVQH010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.128"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            94..1179
                     /locus_tag="OQM87_003423"
     CDS             94..1179
                     /locus_tag="OQM87_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1509436.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            1250..1858
                     /locus_tag="OQM87_003424"
     CDS             1250..1858
                     /locus_tag="OQM87_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCS1509437.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1956..3347
                     /gene="selA"
                     /locus_tag="OQM87_003425"
     CDS             1956..3347
                     /gene="selA"
                     /locus_tag="OQM87_003425"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCS1509438.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVTHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3344..5188
                     /gene="selB"
                     /locus_tag="OQM87_003426"
     CDS             3344..5188
                     /gene="selB"
                     /locus_tag="OQM87_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCS1509439.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            5378..6529
                     /gene="yiaY"
                     /locus_tag="OQM87_003427"
     CDS             5378..6529
                     /gene="yiaY"
                     /locus_tag="OQM87_003427"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCS1509440.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            6694..8232
                     /gene="aldB"
                     /locus_tag="OQM87_003428"
                     /pseudo
     CDS             6694..8232
                     /gene="aldB"
                     /locus_tag="OQM87_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
     gene            complement(8451..8594)
                     /locus_tag="OQM87_003429"
     CDS             complement(8451..8594)
                     /locus_tag="OQM87_003429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509441.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            8777..9100
                     /locus_tag="OQM87_003430"
     CDS             8777..9100
                     /locus_tag="OQM87_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1509442.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9106..10242
                     /locus_tag="OQM87_003431"
     CDS             9106..10242
                     /locus_tag="OQM87_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1509443.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(10239..>10806)
                     /locus_tag="OQM87_003432"
     CDS             complement(10239..>10806)
                     /locus_tag="OQM87_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1509444.1"
                     /translation="AFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYASQRY
                     LEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNTQSA
                     LELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRVQYV
                     LDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2576 a         2656 c         2975 g         2599 t
ORIGIN      
        1 aatcgccgca ctttctgacc gaatttcccg gctggagcat caactcgccg ccgagaaaaa
       61 gactgactat tccacgttcc cggagatcta attatggatc tattgattgt tttaacttac
      121 gtggcgctgg cgacggcggc gctgggaggc gtgtttctgg tgagtggttt gattttgttg
      181 atgaactaca accaccctta cacttttacc gcgcaaaagg cagtgatagc gatccctatc
      241 acgccacagg tgacgggaat tgttactgaa gtcactgaca agaataatca gcttattcaa
      301 aagggcgagg tgctttttaa gctcgacccg gttcgttacc aggcgcgagt tgacagactt
      361 caggctgacc tgatgacggc gacgcataat ataaagacgc tgcgtgcgca gctcactgaa
      421 gcgcaggcca acaccaccca ggtttcagcg gagcgcgacc gtctgtttaa aaattatcaa
      481 cgttacctga aaggcagcca ggcggcggtg aatccgttct cggaacgtga catcgacgat
      541 gcgcggcaaa atttcctcgc gcaggatgcg ttagtgaaag gctcggtggc ggagcaggcg
      601 cagatccaga gtcagctcga cagtatggtc aacggcgagc aatcgcagat tgtgagctta
      661 agagcgcaac ttactgaagc aaaatataat cttgagcaga ctgtcattcg cgcaccaagc
      721 aatggctacg tcactcaggt actgatccgc ccaggtacat acgcagctgc cttgccgctg
      781 cgtccggtga tggtcttcat ccccgagcaa aaacggcaaa ttgtcgccca atttcggcaa
      841 aactcgctgt tacgtctgaa acctggcgat gatgcggaag tggtgtttaa cgcgctacct
      901 gggcaggtgt tccacggcaa attgactagt attttacctg tcgtgccagg cggttcttat
      961 caggcgcagg gggtattgca atcattaacg gtcgttcccg gcacggacgg tgtgctggga
     1021 accattgaac tggaccctaa cgatgatatc gatgccttac ccgacggcat ctacgcccag
     1081 gtggcggttt actccgacca tttcagccat gtttcggtga tgcggaaagt gctgctaaga
     1141 atgaccagtt ggatgcatta tctttatttg gatcattgag acttatatca cctgtgtttt
     1201 gcgtgagcct gagccatact gatatttttc ctcgcacaaa ggactcggca tgaaactcgt
     1261 cggtagctac accagcccgt ttgtacgcaa actttctatt ctgttgttag aaaagggcat
     1321 aactttcgaa tttattaatg aactgcccta taacgcggat aacggcgtgg cgcaatttaa
     1381 cccgttaggg aaagtgccgg tgctggtgac cgaagagggc gaatgctggt ttgattcgcc
     1441 gatcatcgct gaatatattg agttaatgaa tgtcgctccg gcgatgttgc cgcgcgatcc
     1501 gctggagtcg ttgcgggtgc gcaaaattga ggcgctggcg gatggcatta tggatgccgg
     1561 gctggtatcg gtgcgtgaac aggcgcgtcc ggcggcgcag cagtctgaag atgaattgtt
     1621 acgccagcgg gagaaaatca accgcagtct ggatgtgctg gaaggatatc tggtcgatgg
     1681 cacactcaaa accgatacgg tcaatctggc gactatcgcc attgcctgtg ctgtcggata
     1741 tctcaatttc cgccgcgttg cgccaggttg gtgtgtcgac cgcccacact tagtcaaact
     1801 ggtcgaaaac ctgtttagcc gcgaaagttt tgctcgcacc gaaccgccaa aggcttgatg
     1861 cgcgatatgt cctcctgacc catctcacgt tacaatccgt ggttatgtta aacgcccttc
     1921 tccgtgtgag agggccttga tcagccaggt ttcctatgac aaccgaaacg cgttccctct
     1981 atagtcaact tccggctatt gatcgcttat tgcgcgatag ctccttcctt tctttgcgtg
     2041 atacttatgg tcacacccgc gtggtggaat tgttgcgtca gatgctcgac gaagcgcgag
     2101 aagtgattcg tggcagccag acgctgcctg cgtggtgtga aaactgggcg caagaagtcg
     2161 atgcccggtt gacgaaagag gcgcagagcg cgctgcgtcc ggtgatcaac ctgacgggaa
     2221 ccgtgctgca taccaacctt gggcgagctt tacaggcgga agccgcggtg gaagccgttg
     2281 cgcaggctat gcgttcgcca gtgaccctcg agtacgatct ggacgacgcc ggacgcggac
     2341 atcgcgatcg ggcgctggcg cagctgctgt gccgtattac gggggcggaa gatgcctgta
     2401 tcgtcaataa caatgcggcg gcggtgttat tgatgttggc ggccactgcc agcgggaaag
     2461 aggtggtggt atctcgcggc gaactggtgg agattggcgg cgcgtttcgt attcccgatg
     2521 ttatgcgtca ggcaggctgc accctacacg aagtagggac caccaaccgc acgcacgcga
     2581 atgattatcg tcaggcggtg aatgaaaata ccgcactgtt gatgaaagta cataccagta
     2641 actacagcat tcaggggttc accaaagcga tagacgaagc tgaactggtg gcgctcggca
     2701 aagagctgga tgttccagtg gtgactgatt taggcagtgg ctcgctggtc gatcttagcc
     2761 agtacggttt gccgaaagag ccaatgccgc aggagttgat tgcggcgggc gtcagtctgg
     2821 tgagcttctc cggcgacaag ttgttaggcg ggccgcaggc agggattatc gttggtaaaa
     2881 aagagatgat cgcccgcctg caaagccacc cgctgaagcg tgcattacgc gcggataaaa
     2941 tgaccctcgc ggcgctggaa gccacgttgc gtctttattt acaccctgaa gctctgagtg
     3001 aaaaattacc gaccctgcgc ctgcttaccc gcagcgcaga ggtcattcaa atccaggcac
     3061 aacgtttaca ggctcccctt gtcacacatt acggcgcgga gtttgcggta caggttatgc
     3121 catgtctttc gcagattggc agtggttcgc tgccggttga tcgcctgccg agcgcggcat
     3181 taacgtttac accccatgat ggacgcggta gccaccttga gtcattagcc gcccgctggc
     3241 gtgaattgcc agtgccggtg attggtcgta tttatgacgg acgattgtgg ctggatttac
     3301 gctgccttga agatgagcaa cggtttttgg agatgttgtt gaaatgatta ttgcgactgc
     3361 cggacacgtt gaccacggca aaacaacctt attgcaggcg attactggcg taaatgctga
     3421 ccgtctgccg gaagaaaaaa agcgcggcat gaccatcgat ctcggctatg cctactggcc
     3481 gcagccggat ggtcgcgtgc ctggttttat cgacgttccc ggtcatgaaa agtttctttc
     3541 caacatgctg gcgggcgttg gtggtatcga tcacgcgctg ttggtggtgg cgtgcgatga
     3601 cggcgtgatg gcacagaccc gtgagcatct ggcgattttg cagctgaccg gtaacccgat
     3661 gctgacagtg gcgctgacca aagccgatcg tgtggacgaa gcgcgtgttg atgaggttga
     3721 acgccaggta aaggaggttc tgcgggaata cggttttgct gaggcaaaac tgtttatcac
     3781 cgcagcaacc gaaggtcggg gaattgatgc cctgcgcgag catctgcttc agttgccgga
     3841 acgcgagcac gccagccaac atagtttccg cctcgcgatt gaccgcgcat ttaccgtaaa
     3901 aggtgccggg ctggtcgtca ccggtacggc gttaagcggg gaagtgaagg taggcgattc
     3961 actctggctg actggtgtaa ataaaccgat gcgtgtacgt gcgctgcatg cgcaaaacca
     4021 gccaacagaa accgccaatg ccgggcagcg tatcgcgctt gacatcgcgg gtgatgcgga
     4081 aaaagagcag attaaccgtg gcgactggct gcttgccgat gtgccgccag agccgttcac
     4141 acgggtgatt gtcgagcttc aaacccatgc accgctgacc cagtggcagc cgctgcatat
     4201 tcaccacgcc gccagccacg tcacgggacg cgtttcactg ctggaagata accttgctga
     4261 actggtcttc gacaccccgt tatggctggc agataacgac cgcctggtat tgcgcgatat
     4321 ctctgcccgc aacacgctgg ccggagcgcg cgtcgtgatg cttaacccgc cgcgtcgcgg
     4381 taaacgtaaa ccagagtatc tgcaatggct ggcttccctc gctagggcac agaatgatag
     4441 cgaagcgtta gccgttcacc tgcaacgcgg cgcggttaat cttcctgatt tcgcctgggc
     4501 gcgccagctc aacggcgaag ggatgcgcga actgctgcaa cagcctggct atattcaggc
     4561 tggttatagc ttgttgaatg cgccggttgc cgctcgctgg caacggaaaa ttctcgacac
     4621 attagcgact tatcatgagc aacatcgcga tgaacctggg cctgggcgcg aacgtctgcg
     4681 acgtatggcg ttgccaatgg aagatgaagc gctagtactg ttgctgattg aaaagatgcg
     4741 cgaaagcggc gacatcctca gtcatcacgg ttggctgcat ctgccggatc acaaagcggg
     4801 cttcagcgaa gagcagcagg ccatctggca aaaagcagag ccgctgtttg gtgacgagcc
     4861 gtggtgggtg cgtgatctgg caaaagagac gggtaccgac gagcaggcaa tgcgcctgac
     4921 gctacgccag gcggcgcagc aaggaataat taccgcgatc gtcaaagatc gttattaccg
     4981 taacgatcgg attgtcgaat ttgccaatat gatccgcgat ctcgatcagg agtgtggttc
     5041 aacctgcgcg gcggatttcc gcgatcgctt aggcgtaggc cgaaagctgg caattcagat
     5101 tctggaatat tttgaccgca ttggctttac gcgtcgtcgt ggaaatgatc atttattgcg
     5161 cgacgcatta ttatttccgg aaagataagg aaatgattaa ttaattttta aaataaatta
     5221 atacaaattt cttatgattt taaataaagc acattgttta atgaatacaa tgtgcttttt
     5281 attagattaa ttttgataat taataataat gatcatgttt tcgaatgaaa atcctcagta
     5341 agctgcccac ccttttttac actttcagga gtgtgttatg gcatcttcaa ctttctttat
     5401 tccttctgtg aatgtcatcg gcgctgattc attgactgat gcaatgaata tgatggcaga
     5461 ttatggattt acccgtacct taattgtcac tgacaatatg ttaacgaaat taggtatggc
     5521 gggtgatgtg caaaaagcac tggaagaacg caatattttt agcgttattt atgatggcac
     5581 ccaacctaac cccaccacgg aaaacgtcgc cgcaggtttg aaattactta aagaaaataa
     5641 ttgcgatagc gtgatctcct taggcggtgg ttctccacac gactgcgcaa aaggtattgc
     5701 gctggtggca gccaatggcg gcgatattcg cgattacgaa ggcgttgacc gctctgcaaa
     5761 accgcagctg ccgatgatcg ccatcaatac cacggcgggt acggcctctg aaatgacccg
     5821 tttctgcatc atcactgacg aagcgcgtca taacaaaatg gcgattgttg ataagcacgt
     5881 gactccgctg ctttctgtca atgactcctc tctgatgatt ggtatgccga agtcactgac
     5941 cgccgcaacg ggtatggatg ccttaacgca cgctatcgaa gcgtatgttt ctattgccgc
     6001 cacgccgatc actgacgctt gtgcactgaa agccgtgacc atgattgccg aaaacctgcc
     6061 gttagccgtt gaagatggca gtaatgcgaa agcgcgtgaa gcaatggctt atgcccagtt
     6121 ccttgccggt atggcgttca ataatgcttc tctgggttat gttcatgcga tggcgcacca
     6181 gctgggcggt ttctacaacc tgccacacgg tgtatgtaac gccgttctgc tgccgcatgt
     6241 tcaggtattc aacagcaaag tcgccgctgc acgtctgcgt gactgtgccg ctgcaatggg
     6301 cgtgaacgtg acaggtaaaa acgacgcgga aggtgctgaa gcctgcatta acgccatccg
     6361 tgaactggcg aagaaagtgg atatcccggc aggcctacgc gacctgaacg tgaaagaaga
     6421 agatttcgcg gttctggcga ctaatgccct gaaagatgcc tgtggcttta ctaacccgat
     6481 ccaggcaact cacgaagaaa ttgtggtgat ttatcgcgcc gcaatgtaat catcattcca
     6541 acaacggctg gcaaattgtc agccgctttt tcaactatct ctgtaatcct tgcccgtaaa
     6601 ttcgtgatag ctgtcgtaaa gctgttaccg actggcgaag atttcgccag tcacgtctac
     6661 ccttgttata cctcacaccg caaggagacg atcatgacca ataatccccc ttcagcacag
     6721 attaagcccg gcgagtatgg tttccccatc aagttaaaag cccgctacga caactttatt
     6781 ggcggtgaat gggtagcccc tgccgatggt gagtattacc agaacctgac gccggtgacc
     6841 gggcagctgc tgtgcgaagt ggcgtcttcg ggcaaacgag acatcgatct ggcgctggat
     6901 gctgcgcaca aagtgaaaga taaatgggcg cacacctcgg tgcaggatcg cgcggcgatt
     6961 ctgtttaaga ttgccgatcg aatggaacaa aacctcgagc tgttagcgac agctgaaacc
     7021 tgggataacg gcaaacccat tcgcgaaacc agtgctgcgg atgtaccgct ggcgattgac
     7081 catttccgct atttcgcctc gtgtattcgg gcgcaggaag gtgggatcag tgaagttgat
     7141 agcgaaaccg tggcctatca tttccatgaa ccgttaggcg tggtggggca gattatcccg
     7201 tggaacttcc cgctgctgat ggcgagctgg aaaatggctc ccgcgctggc ggcgggcaac
     7261 tgtgtggtgc tgaaacccgc acgtcttacc ccgctttctg tactgctgct aatggaaatt
     7321 gtcggtgatt tactgccgcc gggcgtggtg aacgtggtca atggcgcagg tggggaaatt
     7381 ggcgaatatc tggcgacctc gaaacgcatc gccaaagtgg cgtttaccgg ctcaacggaa
     7441 gtgggccaac aaattatgca atacgcaacg caaaacatta ttccggtgac gctggagttg
     7501 ggcggtaagt cgccaaatat cttctttgct gatgtgatgg atgaagaaga tgcctttttc
     7561 gataaagcgc tggaaggctt tgcactgttt gcctttaacc agggcgaagt ttgcacctgt
     7621 ccgagtcgtg ccttagtgca ggagtcaatc tacgaacgct ttatggaacg cgccatccgc
     7681 cgtgtcgaaa gcattcgcag cggtaacccg ctcgacagcg tgacgcaaat gggcgcgcag
     7741 gtttctcacg ggcaactgga aaccatcctc aactacattg atatcggtaa aaaagagggc
     7801 gctgacgtgc tcacaggcgg gcggcgcaag ctgctggaag gtgaactgaa agacggctac
     7861 tacctcgaac cgacgattct gtttggtcag aacaatatgc gggtgttcta ggaggagatt
     7921 tttggcccgg tgctggcggt gaccaccttc aaaacgatgg aagaagcgct ggagctggcg
     7981 aacgatacgc aatatggctt gggcgcgggc gtctggagcc gcaacggtaa tctggcctat
     8041 aagatggggc gcggcataca ggctgggcgc gtgtggacca actgttatca cgcttacccg
     8101 gcacatgctg cgtttggtgg ctacaaacaa tcaggtatcg gtcgcgaaac ccacaagatg
     8161 atgctggagc attaccagca aaccaagtgc ctgctggtga gctactcgga taaaccgttg
     8221 gggccgttct gatataagaa gctggtcgca ttgggtattc attgcctgat gcgacgctta
     8281 cgcgtcttat catacctacg ggaacctgac cgtaggccgg ataaggcgta cacgccgcat
     8341 ccggcataaa caaagcgcac gttgttacaa tttacgtttt aaacctacgt gcgcaataac
     8401 ccagactacg ccaacagaag tgcgtttctg gggcgtgttt tgtcaggagt ttaatctcgc
     8461 gtttcgatgg tgagcgggcg aaccaggccg taaatcacca ttggtgatcc gaggccaatc
     8521 agcaataaac ggtagataat tgaaggttta atacgcttaa tctgtactgt ggatgtttta
     8581 ctggagttat tcattagttt ggccaatagt tccactattt caatatgata aacctcgtcc
     8641 tgttgaaaaa ccagacagca catttcagaa agtatataaa aaacaatatg tcgttatata
     8701 aataaggaaa ttgcactgca tcatggcatt tcataatatg cgttaattgt tcttttttat
     8761 attctttgag ggaaatatgt tcctggacta ttttgcactg ggagtgctta tttttgtatt
     8821 tctggtgata ttctatggga ttattatttt acatgatatt ccctacctga tttctaaaaa
     8881 acgtaatcat cctcatgccg acgccattca tgttgctggt tgggtgagtc tttttacgct
     8941 gcatgttatc tggccatttt tgtggatttg ggccacgctt taccgcccgg agcggggatg
     9001 gggaatgcaa atccatgact cctccgttgt gcaactacaa cagcgtattg ccgggctgga
     9061 gaaacagctc gccgacatca aatcctcttc tgccgagtaa tacttatgga tttactgatt
     9121 attttgacct atgtggcttt tgcatgggca atgtttaaga tcttcaaaat tcctgtaaat
     9181 aaatggacca tttccacagc ggccctgggt ggcatattta ttgtcagtgg tttaattctg
     9241 ttaatgaact ataaccatcc gtataccttc aaggcgcaaa aagcggttat ttctattcct
     9301 gttgtcccac aggtgacagg cgtggtgatc gaagtgacgg ataagaaaaa tacgctgatt
     9361 aaaaaaggtg aggtgctatt tcgactggac ccgacgcgtt atcaggcgcg ggtggatcgg
     9421 ctgatggcgg atatcgttac cgcagaacat aaacagcggg cgttgggcgc agagttagat
     9481 gagatggcgg cgaatactca gcaggcaaag gccacgcggg ataaattcgc taaagagtat
     9541 cagcgttacg cacgcggcag ccaggcgaaa gtaaacccgt tttcagaacg cgatattgat
     9601 gtggcgcggc aaaattatct ggcgcaggaa gcctccgtaa agtcatcggc ggcggaacaa
     9661 aaacagatcc agagccagct ggatagcctg gtgttgggtg aacattctca aatcgccagc
     9721 ctgaaagcgc agctcgcgga agcaaaatat aaccttgagc agacgatagt gcgtgcgccg
     9781 agcgatggtt atgtgaccca ggtgctgatt cgtccgggta cctatgccgc gtcgctgccg
     9841 ctacgtccgg tgatggtgtt tatacccgat cagaaacgac aaattgtggc gcagttccgt
     9901 cagaactcct tgctgcgact ggcccctggc gacgatgcgg aagtggtgtt taatgctctg
     9961 ccaggtaagg tattcagcgg caagctggca gccattagtc cagctgttcc cggcggagct
    10021 tatcagtcga ccggcacctt acagacgtta aacacagcgc cgggttcaga tggcgttatc
    10081 gcgaccattg aactggatga gcacactgat ttgagcgctt taccagacgg tatttacgcc
    10141 caggtggcgg tctactctga tcatttcagc catgtctcgg tgatgcgcaa agtgctatta
    10201 cgcatgacca gctgggtgca ttacctttat ctcgatcatt aatcttcgct ttccgccgct
    10261 tgttcaggcc gcagttgctg cgactgagca agctggtggc gaatttcacg cagcatttca
    10321 tccagcacat actggacccg ccagggcagg tattcccgtt tacggaagat agccaccaga
    10381 tccagccacc attcatacgg gtgctgaaag cagggcacca gcgtgccgtc ttgcagatcg
    10441 ctctgcacac ttttgtctgg cgcaaacaca atccccaggt gatttcgtgc cagctccagc
    10501 gccgattgcg tgttatcgca aacgtaattt cccttcacgc gataatcccg cacctcttta
    10561 ctcccggcga cgttgaagcg ccagatattc gcgtcgtcga tcatcatcga gtcgatcaaa
    10621 atacaggagt gatgttcgag ttcgtcagga cggctaatag gatgtttctc aagatagcgc
    10681 tggctggcat aagcggttac ggcatattgt gtaataaaac tggcaaccag cgattcatct
    10741 tttggtggag cgtagctgat taaaacatca caatcatcag gaaattcgac gccttcataa
    10801 aaggca
//
LOCUS       DAJVQH010000129        10462 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJVQH010000129 DAJVQH010000000
VERSION     DAJVQH010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.129"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="OQM87_003433"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="OQM87_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCS1509445.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAEMVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="OQM87_003434"
     CDS             complement(1151..1237)
                     /locus_tag="OQM87_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCS1509446.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OQM87_003435"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OQM87_003435"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1509447.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OQM87_003436"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OQM87_003436"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCS1509448.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="OQM87_003437"
     CDS             3586..3630
                     /locus_tag="OQM87_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509449.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OQM87_003438"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OQM87_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCS1509450.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     EMEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGCDKGHQWMEEQLVSICKR"
     gene            5389..5475
                     /gene="leuL"
                     /locus_tag="OQM87_003439"
     CDS             5389..5475
                     /gene="leuL"
                     /locus_tag="OQM87_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCS1509451.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5568..7139
                     /gene="leuA"
                     /locus_tag="OQM87_003440"
     CDS             5568..7139
                     /gene="leuA"
                     /locus_tag="OQM87_003440"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCS1509452.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7139..8230
                     /gene="leuB"
                     /locus_tag="OQM87_003441"
     CDS             7139..8230
                     /gene="leuB"
                     /locus_tag="OQM87_003441"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCS1509453.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8233..9633
                     /gene="leuC"
                     /locus_tag="OQM87_003442"
     CDS             8233..9633
                     /gene="leuC"
                     /locus_tag="OQM87_003442"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCS1509454.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9644..10249
                     /gene="leuD"
                     /locus_tag="OQM87_003443"
     CDS             9644..10249
                     /gene="leuD"
                     /locus_tag="OQM87_003443"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCS1509455.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10298..>10462)
                     /locus_tag="OQM87_003444"
     CDS             complement(10298..>10462)
                     /locus_tag="OQM87_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1509456.1"
                     /translation="AATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVA
                     LFLTAKVKDV"
BASE COUNT         2736 a         2745 c         2561 g         2420 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggcttagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccatc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccagcc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aagcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccctgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaatgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 acccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccatttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgtttttgtt gcgaaaacaa tctaatcata actaccgcga atactcaatc
     4981 atctacaaaa tggattaaat gtgcttattt aataattatt ttatatattt aatgcattaa
     5041 ttcttaacat taattgatca ataatattca ccaaatcaat atcaaaaaaa atcgcaaaac
     5101 atataattca atacaaatca ttaggatagg ttttgcaacg cgtgcatttt gtcccctttt
     5161 tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg
     5221 tagttaagca gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta
     5281 cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
     5341 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
     5401 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
     5461 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
     5521 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
     5581 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
     5641 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
     5701 tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg
     5761 ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
     5821 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa
     5881 tgcacatcgc caccaagctg cgcagcaccc tggacgaggt gatcgaacgc gctatctata
     5941 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
     6001 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
     6061 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
     6121 gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg
     6181 acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg
     6241 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca
     6301 tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccagg
     6361 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     6421 aagccattgt tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc
     6481 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     6541 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
     6601 ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg
     6661 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     6721 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     6781 tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg
     6841 gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg
     6901 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     6961 atattgtcgc caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     7021 tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag
     7081 tcgaaaaaga gttgcaacgc aaagctcaac acaacgaaaa caacaaggaa accgtgtgat
     7141 gtcgaagaat taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac
     7201 ccaggcgctg aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
     7261 ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac
     7321 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
     7381 ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca
     7441 cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg
     7501 tccgctgcgt gcagacattg ccgctaacgg cttcgacatc ctgtgcgtgc gcgaactgac
     7561 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     7621 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     7681 atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc
     7741 ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact
     7801 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     7861 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     7921 tggctcgatg ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga
     7981 accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca
     8041 aatcctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc
     8101 cattgaacgc gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg
     8161 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     8221 aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac
     8281 gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     8341 tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     8401 accttcgcca ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt
     8461 gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag
     8521 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
     8581 gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
     8641 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa
     8701 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     8761 ggcattaccg caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc
     8821 accgggcatg tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt
     8881 atgaccctgt gcaatatggc gatcgaaatg ggcgcaaaag ccggtctggt tgctcctgac
     8941 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     9001 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
     9061 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag
     9121 gtgatttccg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt
     9181 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
     9241 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact cgcgcattga agatttacgc
     9301 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
     9361 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt
     9421 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     9481 cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag
     9541 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc
     9601 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     9661 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     9721 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
     9781 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
     9841 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     9901 tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt
     9961 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
    10021 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
    10081 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
    10141 cttccgccgc cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga
    10201 cgacgcgatt gcgtcttatg aagcaaaaca acctgcgttt atgaattaga ccccttgccc
    10261 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
    10321 ttaaaaataa tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc
    10381 aactttgtgc aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt
    10441 tggtaaataa ggtggtagct gc
//
LOCUS       DAJVQH010000130        10403 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJVQH010000130 DAJVQH010000000
VERSION     DAJVQH010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.130"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..172
                     /locus_tag="OQM87_003445"
     CDS             <1..172
                     /locus_tag="OQM87_003445"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCS1509457.1"
                     /translation="KPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGDRLHS
                     RKLRTFNEPPFR"
     gene            515..1231
                     /locus_tag="OQM87_003446"
     CDS             515..1231
                     /locus_tag="OQM87_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1509458.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLDAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1687..1762)
                     /locus_tag="OQM87_003447"
     tRNA            complement(1687..1762)
                     /locus_tag="OQM87_003447"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1727..1729),aa:Asn,seq:gtt)
     gene            complement(1863..3490)
                     /gene="yeeO"
                     /locus_tag="OQM87_003448"
                     /pseudo
     CDS             complement(1863..3490)
                     /gene="yeeO"
                     /locus_tag="OQM87_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            3495..3570
                     /locus_tag="OQM87_003449"
     tRNA            3495..3570
                     /locus_tag="OQM87_003449"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3528..3530,aa:Asn,seq:gtt)
     gene            complement(3608..4558)
                     /gene="cbl"
                     /locus_tag="OQM87_003450"
     CDS             complement(3608..4558)
                     /gene="cbl"
                     /locus_tag="OQM87_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCS1509459.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(4660..5577)
                     /gene="nac"
                     /locus_tag="OQM87_003451"
     CDS             complement(4660..5577)
                     /gene="nac"
                     /locus_tag="OQM87_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCS1509460.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            5904..5979
                     /locus_tag="OQM87_003452"
     tRNA            5904..5979
                     /locus_tag="OQM87_003452"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5937..5939,aa:Asn,seq:gtt)
     gene            complement(6035..6970)
                     /gene="ldtA"
                     /locus_tag="OQM87_003453"
     CDS             complement(6035..6970)
                     /gene="ldtA"
                     /locus_tag="OQM87_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1509461.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(7032..8111)
                     /gene="cobT"
                     /locus_tag="OQM87_003454"
     CDS             complement(7032..8111)
                     /gene="cobT"
                     /locus_tag="OQM87_003454"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCS1509462.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(8123..8855)
                     /gene="cobS"
                     /locus_tag="OQM87_003455"
                     /pseudo
     CDS             complement(8123..8855)
                     /gene="cobS"
                     /locus_tag="OQM87_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
     gene            complement(8852..9397)
                     /gene="cobU"
                     /locus_tag="OQM87_003456"
     CDS             complement(8852..9397)
                     /gene="cobU"
                     /locus_tag="OQM87_003456"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCS1509463.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(10024..10287)
                     /locus_tag="OQM87_003457"
                     /pseudo
     CDS             complement(10024..10287)
                     /locus_tag="OQM87_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2959 a         2630 c         2209 g         2605 t
ORIGIN      
        1 taaaccgtta catggtgaga ttaagcttcc cggccaggct aaccgttttt atgaaggcgc
       61 tatttccgaa cacctgcaaa ttggcattcg ggcgatcgat ttgctgcgcg cagaaggcga
      121 ccgactgcat tcacgtaaat tacgaacctt taatgagccg ccgttccgat aataagttat
      181 gaaccttctt atcagaaata gatgtgaagg attaagtaag acatctggca aatagcctgc
      241 aataaacgcg ggctgcttgc ttccttgggc agatgcgagt tttattatcg tcttaatgat
      301 ttccacatat taaaagcaag tatgctttca aaacacaatt ataaaaaatc ccgccaacaa
      361 cattagtttt tataaaatta aatataagat tatggcttta gaatattttt atttctaata
      421 gacgagattt ttcctgttat gatataatat gccgaattaa cacatgttaa cgatttacca
      481 gtaatgtaaa taaattttcg aggagatcat tccagtggga cgtaaatggg ccaatattgt
      541 tgctaaaaaa acggctaaag acggtgcaac gtctaaaatt tatgcaaaat tcggtgtaga
      601 aatctatgct gctgccaaac aaggtgaacc cgatccagaa ttaaacacat ctttaaaatt
      661 cgttattgaa cgtgcaaagc aggcacaagt tccaaagcac gttattgata aagcaattga
      721 taaagccaaa ggcggcggag atgaaacgtt cgtacaggga cgttatgaag gctttggtcc
      781 taatggctca atgattattg ctgagacatt gacttcaaat gttaaccgta cgattgctaa
      841 cgttcgcaca attttcaata aaaaaggcgg caatatcgga gcggcaggtt ctgtcagcta
      901 tatgtttgac aatacgggtg tgattgtatt taaaggaaca gaccctgacc atatttttga
      961 aattttgctt gacgctgaag ttgatgttcg tgatgtaact gaagaagaag gtaatattgt
     1021 tatttatact gaacctgctg accttcataa aggaatcgcg gcgctaaaag cagctggaat
     1081 cactgagttc tcaacaacag aattagaaat gattgctcaa tctgaagttg agctttcccc
     1141 agaagattta gaaatctttg aagggcttgt tgatgccctt gaagatgacg acgatgtaca
     1201 aaaagtttat cataacgtcg caaatctcta attatatttt aaagaaatct gtctttatgg
     1261 cagatttctt taatctcata taattcttat aaaaaatata atgtttaact cgtcatattg
     1321 cttataaaaa atccccgttc ccagagaaat aatatttatt aaaatggcag gtcttctttt
     1381 tctgattacc gaagacaaag tggcaattac gtatagtttc ccgataaaga cgcgacagct
     1441 acatcccgca taaggcattt ttctctttat ctttgtacgg tacttcatgg aacagaattt
     1501 ttgaccttgt gaatcgtgat gtctgttggg gaggggcaat ttgctcactg aagcgtgaga
     1561 ctcgattaag cgcacgaaac acagaaatca aaaaacccgg ccactttttt acaaagtggc
     1621 cgggtaaaaa taatttttat tttttaactg tcctgagtct catagagatg tctcaaaact
     1681 aaaatttggc tcctctgact ggactcgaac cagtgacata cggattaaca gtccgccgtt
     1741 ctaccgactg aactacagag gaatcgtgag aacgaggcga atattagcga tgcccaccca
     1801 caatgtcaaa gcctgttttt taaatttgaa atcgtttgct gaaataatct gcattttgtc
     1861 gtttattccg acacaactgg ctttttttca cacttttgcg gctcggatcg agggtatttc
     1921 cacagccaac gtccagtaac cattcgccag taaaacagca cggcccgcac agcccagtag
     1981 gcaaacattc ccatccagac accaaccaca ccccagccaa gcatgattcc ctgcacataa
     2041 ccgactacaa cccggcaacc ccacatgctc aacatagaaa cccacatggc gtaacgggca
     2101 tcacgagcac ccttaaatcc agcgggtagc acccatgagg cggaccaaat aggcataaat
     2161 aaagcattta gctaaatcag aatcacaacg acgtgtttaa cctgtggatc ctgggtgtaa
     2221 aacgatgcca taaccccggc aaagggagcc gttagccagg cgatggccgt taatccaaga
     2281 gtggacagcc agaacacatg ccgcaactga atctctgctt gcgctatctg ccctaccccc
     2341 aaccttcggc ctgtaatgat cgtagaagca gaaccgagcg cacttccggg taagttgata
     2401 agagccgcaa ttgagaacgc gataaaattt ccggcaataa cgctggtccc catcccggca
     2461 acgaacattt gggttaataa ccgaccactg gtaaataaca ctgattcgac actcgctgga
     2521 ataccaatcc ccatgacttc ccagataatg ctaaaattca gcggtttaaa atagctcttt
     2581 aacgaaatcc ttagcgcagg attaaaacca atcgccagca cccacaaaat tgcaactgcg
     2641 ccaatataac gagaaatggt taaacccaac ccagccccga ccaggagaaa aggccgtaaa
     2701 tcaatatgcc gctaataata atattaagaa tattcaggct accgttaatc agtagcggta
     2761 ttttcgtatt ccctgcacca cgaagtgccc cgctaccaat aagagtgatg gcagcggctg
     2821 gataactgag tacggtcagc tccagataag tcaacgccag tgctttaact tctgtcgtgg
     2881 catcacccgc gacgaaatca ataatttgtt cgccaaaatg atgaattagc gttgccaaca
     2941 gtacggcaaa caacgtcatg atcaccaatg actgccgcgt cgccaccctc gctcgtcgtc
     3001 gatcccgctt accgagacta aatgctacaa cgacagtagt accaagatcg atagctgcaa
     3061 aaaaagccat aatgaccata ttgaagctgt ccgccaaccc cacgccggcc atcgcatctt
     3121 ttcccagcca gctgaccaga aaagtgctca ggccccccat caacaggaca cagacgttct
     3181 ccatgaagat aggaacagca agcggggtta tctcgcgcca gaacaacact ttgtagctct
     3241 tgcgtttaga gtgccagcga gtgccgtgaa caacctggcg taaagcagag gagatattca
     3301 aagccgacct taattgcaga aagtgaaacc acatttcaaa tgatgaggga gaatcagcaa
     3361 agctgcaaag attttcgcca acaaattgtc tgcaaatgca acaaactgtt gatagaaacg
     3421 gcaaacagtt gggaaattta aaaatcgggt ttgacaaaat atttttcgcc gttaagatgt
     3481 gcctcaacag cgattcctct gtagttcagt cggtagaacg gcggactgtt aatccgtatg
     3541 tcactggttc gagtccagtc agaggagcca aatttctgaa aagcccgctt ttatagcggg
     3601 ctttttgcta tatctgataa tcaatttcct cttcactgtt ttccatcacc tggcgcttga
     3661 tatcctcaac tgacagtcct gcattacaaa gttccagaaa gcgccaaaca tagttacgct
     3721 gaagttgtcc tcgcttcagt cccaaccaga cagtattagc atcgaaaaga tgccgcgtat
     3781 ccaggcggat taaattctct tcctcttgtt cgccgctgga ttgttcggca actaatccga
     3841 tcccaagccc aagagcaaca taggttttaa tgacatcaga atcctgtgca cttaatacaa
     3901 tatctgccgg caaacctttg cgggtaaatg cgtcatcagt gcgtgagcgc cccgtaattc
     3961 cctgtcggta agtaattaac ggccacttcg ctattgattc cagcgtcaat ggtgtaattt
     4021 gcgttaaggg atgatcgagt ggaacaagca aactatggtg ccaacaaaac cacgggaagg
     4081 cgacgagctg cgggtcatta ctcaaacgct cgctggcgat accaatatca gcttcgccat
     4141 tttgcaacaa cgtcgcaatt tcctgtggcg aaccctggat tagctcgagc cgaacctccg
     4201 ggaaaagttc gcgaaaagct ttaatgacct ctggcaagct ataacgtgcc tgagtatgcg
     4261 tcgttgcaat agtgagaaca ccagacgtat cgttggtaaa caggtctgca agccgacgaa
     4321 cattactggc ttcattcaga atacgttctg caatgaccag taatgctttg cccggttcag
     4381 tcatccccag cagtcgctta cctcgtcgaa caaatatttc gatgccaagt tcatcctcca
     4441 gttcccgaat atgacggcta acgcctgact gtgaggtaaa aagcatattc gcaacctctg
     4501 tcaggttgta atcctgacgt gcagcctcgc ggattatctt tagttgttgg aaattcacgg
     4561 taaactccgg gcagttcaga tatcccgtta ttgttaaagt ctaatgcccg gcataacaaa
     4621 taataaaaac ccgcatctta ttccatcccg atataacgct tagctcacca attgccactg
     4681 ccttttttcc atcactggag aactaatcac tgacattaac aactctttca ctgcctgtgc
     4741 ctgtggtgac aagtttgctc tggcgggtaa atttaaggac aaagagagac tcattgaagg
     4801 agtggtaatg cgtgacatcc acccatttac tgcgccacat aacgaacgcg cagccgattc
     4861 aggtaatact gcaacgccca tgccgctggc aatcgctgcg gtaagcgtgg caatagactc
     4921 aatttcacca atgacttttg ccgtgagccg ccgtagggaa aaagcctcat caacacgaag
     4981 tctaacagcg ctgtaatcac tggggagaaa aaggttcatt tgcgcaatag cattaacatc
     5041 aacgttttgc cccgggcaat cttgagttcc taccaggaaa agatcttctt tcagcaaagc
     5101 ctgactggat accccagcca caggggaatg ctcataaatc accgccatat cgagttggtg
     5161 atttatcagt ttttcgttaa gcactgcgcc actattttca tgaagataga taacgacctc
     5221 cgggaattca gcgcgaaccg cctgtaataa gggcatggtg atggatgacg cagcggttcc
     5281 tggcgcaaag ccaatcgaga cttgccccga taatgactga ccaacgttat gcaccgccag
     5341 ttgggcctgc tcacactgac gtaaaatggc ccgtgcatgg gtatagagaa tttttccggc
     5401 gtctgttggc gtaacgcccc gttttgtgcg aatcaaaagc tgttgattta actcaccttc
     5461 cagtatggca acctgctggc tgagcgctgg ttgtgcgata tgcaatactt cagcagcctg
     5521 ggtcaggcta ccaatatcta caatttttac gaagtatttc aggcgtctga agttcatgtt
     5581 gcctccggtt tttaagaatc ggcccaagtg ccgccattac ttacaaccag tatgcaagat
     5641 gcctgccagt tctattttgg cgttgatgcg caagctaacc aactgtcaaa taagagatta
     5701 tgatagattc gtcattggcc ctttttatca gcagtcgcat tcacctgccc tataaaagga
     5761 gggtatgcac cacgatggtt cattacccaa taagattgaa agctcaccac tttgttgaaa
     5821 ttggcagcaa acaaacaaaa aaatgcattt caccctttga catcaccatg cactgccatt
     5881 aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta
     5941 atccgtatgt cactggttcg agtccagtca gaggagccaa attcaaaaaa gcctgctttc
     6001 gagcaggctt tttactttct aattaccaac gctcttaaaa catctgtctt gaaccagaac
     6061 taatttgcac aggcattccc gatcgatgtt gcaacgcagc atttgcgcga tttacatcaa
     6121 cttcttgccc gttgataaac gcccgcaaag atggggttac cggcaatggc acttttcggt
     6181 cagactcata ttctgcacga ttgcgtgaca gcggctcatg aacttccagc cactttgaac
     6241 catccggttc cgttgtgtat ttcactggct gatcaataat ttgtacacgt gtgcctacag
     6301 gaacattatc aaacagatat ttgatatcgt cattacgcag acgaatacag ccctgactta
     6361 cacggagccc aataccaaaa ttggcattgg taccgtggat ggcatacaac ctgccaatat
     6421 aaatcgcgta cagccccatg gggttatcgg gccccgcagg aacaaatgcg ggcaaactct
     6481 cccctcgttt cgcatattcg cgccgggtgt tcggcgttgg cgtccaggtt ggtgcttctt
     6541 gtttacgttc aacggtagtc acccagttac gcggggtttc tcgcccagcc tggccgatac
     6601 caataggaaa gacttccact gtattactgt ctggcggata gtaataaaga cgcatttcag
     6661 cgacgttaac cacaatccct ttacgaacag tggcgggcaa aatcagttgc tgcggaatgg
     6721 tgagttgtga gccaggcttc ggtaaaaaaa catcagcgcc cggattcgct tccagcatat
     6781 tacttaaccc ttgcccgtat tgtgcggcaa aagtctccag cggctgagta ttgtgatcag
     6841 gaacagttac agtaagcgac tgccccacta aacggctacc ctctggaggt aatggataag
     6901 ttaccgccag gctattttgg ctggcaaaaa gcagagcaaa tgagcaaaga atatttacac
     6961 gacgcatcat gtccctttcc taagtcgcga atactatccg ataagtatag cttttatcag
     7021 acttttcgtt tttagctgtt caaatcagaa gtcgtattcc ccggtagaac aatattactg
     7081 gcagcaagtt cgcccatgtt gttgtatatc gcacaagcag cttcgatgat ggacatcgcc
     7141 agagctgcac cactcccctc acctaaacgc atatccatat tgagataagg ctccagcccc
     7201 aaatgcgaga gcgctatacg cgcgcctttt tctgccgaca agtgagaagg aatgagatac
     7261 ggtttgattg caggagacat ctggcaggct gcgagcgcag cagcataaga aagaaatcca
     7321 tccagcaaca cgggtaaacc gcaggaagca gcacctaaca tcactccagc tattccgacc
     7381 aaatcaaatc cacccacttt tgccaggaca tcaacaccat cctgaggatt tggttgattc
     7441 aacgtaatcg cccgacgcac aacatcaatt ttattagcca gtttatctgt cggcaggttt
     7501 gcgccaatcc caaccacttc ttcaggatcc cggccagtga ttgtgctgac tattgccgct
     7561 gccggtgtcg tgtttgccat ccccagttca cctacaccaa acagcgtgac accgtttttc
     7621 gccagctccc gcgtataaca tatgacgtcc aaaagcaact tttcagcctg acggcgactc
     7681 attgccggag ctgaagcaat attgccgcta cctcgtgcga cacgcatgtt gataagcccg
     7741 gggataggct cagcagtatc aataccaaca tcaattacgt ggacgttagc gcccgcttgt
     7801 gctgccagca cacacacgcc ggttgttcca cgggtcatat tttcagcctg tatggctgtc
     7861 acttcttttg gagaaatagc gaccccttcc tcccagacgc cgtgatcggc acacataacc
     7921 agtaccgctt ttttgcccac atgcggtatg ccattcaccc ccggcattcc tgccagttgt
     7981 atggcaagcg cctccagctt tcctaggcta ccaacaggtt tgagtaaccc gtcaacatgc
     8041 cgttgtgcac gcgacatagc ggcagaattg atggctggga tcgtattcag taaatcggca
     8101 attatttgca tgtcatgtct ctttatagca gtgccagcag gaagaccagt tcaccaagct
     8161 ctatggctgc tcccagcgta tcgcccgttt gcccgcccag cgtacgtttg agaagctgac
     8221 cgaggatgaa aatcgccacc atcgtcacca ccatagcagc cacaccatgc atacccagca
     8281 ataatacggc agcaaaaata gcggctaagc cgagtgtgac gcaggtttgt cgcgccaatg
     8341 aacacattgc caagcccttc ttcccgcgca taacgatgac gatacatcaa taatgcggca
     8401 gtcccgcggc tgaccgcaca tgctgccgcc agcgatgcaa ggatcggttc gccacgcaga
     8461 gccaactcgc ttaacaccag aatctttgcc agtaccacaa aaatcaatgc cagaccgccg
     8521 tgggtgccta aacggctatc acgcatgatt tccaacatgc gatcgcggct acgtgcagaa
     8581 aatacgccat cacaggtatc agcgaggccg tccagatgga attccccggt catcagcgcc
     8641 agcacaagta cgctaaacag tgccgccagt ggtgcgccac accatgcctg cagcaccatg
     8701 aagaccagcc cgctaatcgc gccaagcaat aatccaatca aaggaaaagt aataataccg
     8761 cgagaataat gctcgaaatc cagtccctgg gaccagcgac gcggtacagg cagacgcgta
     8821 ataaatgaga gcatcgccca aaataatttg ctcatttgat ttttactcca atacccgaaa
     8881 ccaccagcca tacttcattt gccgcagcgg ccaactgctg atttacccgc ccggcaatat
     8941 cacgaaaatg tcgtgccaga cgactctccg gcacaatccc cattcccact tcgttagtca
     9001 ctaatacaac ctttgcgggg caacgttggc aggcagcaat caacgactga atctcagcat
     9061 taatcgcctg ttccatcgcc tgataatccc attcatcagg gtctttatcg ccgccataat
     9121 caaacaacag attagtcacc attgtggtaa cgcattccag caacacagcc tcattcgggt
     9181 taatgtctgc atgaattaat tcatcaagat gttgccagcg ctccactgtg cgccagtgct
     9241 ccgggcggct ttgccgatga tgttctatcc gtgcagccat ctcatcatca aggatttgcg
     9301 aggtagcgat atacagaacc tgtgaagagt ccccaataag cacctctgca tggcgactct
     9361 tcccgctcca tgcgcctccc gtcaccaaaa tcatcatacc gactcctgat tcaaatgtat
     9421 gatggtatac attgaacaga gcaatggatc ccccaccgtt gctgaccgca aaacatttac
     9481 gcgattaata ctgcgcgtaa tataattaaa aatccagcat tctcaatcca ttacgaaata
     9541 agatggcatt acggaatagc cagactttct cgtgttaacg tccgacaaaa tgcgtcattg
     9601 acagcggttt ctgtggattg acacatttca ttcagataat gaattaatgc tactgccgga
     9661 ccaaccagca aacgggcatt gtgttctgaa atccaggcta ttgattcaac gtcagcgaca
     9721 gatctgcgct gtaaaagaac ttgtaaaaca acgcaataga attatccgga atggtggcga
     9781 ctatgcacta gaacaccaca atttcgctct ctcggtaagg cactgtcaca gcattcaaca
     9841 gaatgtgact tgccagattt attagcgcca ccagatgttt aaccgggtaa ccacgaccca
     9901 gtcggggata tattccatct tctcgctgac atcatcaaga ataaaaaggt ttatcacact
     9961 cagagcaacg ttcttcagca ggcgggataa ttcttcaaca tttaaaagat gtgttattaa
    10021 aggcatctca aattggacag ggctgccggt tttctgttgc aacaccgttg ctctgcttga
    10081 gacagaactg ccatatgcag catctgatac tttgagtttg accaaatcac agccccgtag
    10141 cttactgtcc aaagccatgt tgaacagagc tagatcgcgt gttttacctt ccaactcaag
    10201 tctaatccgg atcccccaga tatgagatat ctgaagtggc cttttttgac cgatggtacg
    10261 gtctttattc cacggtgacg ttttcatatt caaatctcct gcaatgtggg agatttgagt
    10321 atggttgtct ctcaagagcg aaaataagtc aactatcact agttgttgcg catgtacgta
    10381 ttctgtcaat taattattga caa
//
LOCUS       DAJVQH010000131         9957 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJVQH010000131 DAJVQH010000000
VERSION     DAJVQH010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.131"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..272)
                     /locus_tag="OQM87_003458"
     CDS             complement(<1..272)
                     /locus_tag="OQM87_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCS1509464.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="OQM87_003459"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="OQM87_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1132..1338)
                     /locus_tag="OQM87_003460"
     CDS             complement(1132..1338)
                     /locus_tag="OQM87_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCS1509465.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1445..2008)
                     /locus_tag="OQM87_003461"
     CDS             complement(1445..2008)
                     /locus_tag="OQM87_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCS1509466.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="OQM87_003462"
     CDS             complement(3383..5218)
                     /locus_tag="OQM87_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509467.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="OQM87_003463"
     CDS             5319..5606
                     /locus_tag="OQM87_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1509468.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="OQM87_003464"
     CDS             5578..7107
                     /locus_tag="OQM87_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCS1509469.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="OQM87_003465"
     CDS             complement(7277..8494)
                     /locus_tag="OQM87_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1509470.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8861..9847)
                     /locus_tag="OQM87_003466"
     CDS             complement(8861..9847)
                     /locus_tag="OQM87_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509471.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFLWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9844..>9957)
                     /locus_tag="OQM87_003467"
     CDS             complement(9844..>9957)
                     /locus_tag="OQM87_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCS1509472.1"
                     /translation="EISNKPYLGNMLNDFAGVDQQRVMHCRKAFSDAKVFK"
BASE COUNT         3138 a         2262 c         1846 g         2711 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgc aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     9001 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataacca
     9061 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     9121 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg taccgcttgg
     9181 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     9241 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcaacg
     9301 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     9361 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     9421 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     9481 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     9541 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct
     9601 ttttcttaaa ccacaaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     9661 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     9721 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     9781 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     9841 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     9901 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttc
//
LOCUS       DAJVQH010000132         9954 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJVQH010000132 DAJVQH010000000
VERSION     DAJVQH010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.132"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(66..1349)
                     /gene="aroA"
                     /locus_tag="OQM87_003468"
     CDS             complement(66..1349)
                     /gene="aroA"
                     /locus_tag="OQM87_003468"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCS1509473.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1420..2508)
                     /gene="serC"
                     /locus_tag="OQM87_003469"
     CDS             complement(1420..2508)
                     /gene="serC"
                     /locus_tag="OQM87_003469"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCS1509474.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2707..3335)
                     /locus_tag="OQM87_003470"
                     /pseudo
     CDS             complement(2707..3335)
                     /locus_tag="OQM87_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3465..5225
                     /locus_tag="OQM87_003471"
     CDS             3465..5225
                     /locus_tag="OQM87_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCS1509475.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5631..6488
                     /gene="focA"
                     /locus_tag="OQM87_003472"
     CDS             5631..6488
                     /gene="focA"
                     /locus_tag="OQM87_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCS1509476.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6543..8825
                     /gene="pflB"
                     /locus_tag="OQM87_003473"
     CDS             6543..8825
                     /gene="pflB"
                     /locus_tag="OQM87_003473"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1509477.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9017..9757
                     /gene="pflA"
                     /locus_tag="OQM87_003474"
     CDS             9017..9757
                     /gene="pflA"
                     /locus_tag="OQM87_003474"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCS1509478.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2553 a         2494 c         2375 g         2532 t
ORIGIN      
        1 gcattacgac aatgtgccga cgttttgata aagaaagatt tggctattta ttgcccgttg
       61 ttcattcagg ctgcttggct aatccgcgcc agctgctcga aataatccgg aaatgttttg
      121 gccgtgcatt tgggatcaag aatcgtcact ggtgtatctg acaacgccac cagcgagaaa
      181 cacatcgcca tccggtgatc attgtatgtc gcgatctcgg caaagttcag tttttccgga
      241 ggagtgatac gaatgtaatc gtgcccctct tccacttccg cgccgacttt acgcagttct
      301 gttgccatcg caaacaggcg atcggtctct ttaacacgcc agttatagat attgcgcagc
      361 gtggtggtgc cttttgcaaa taacgccgcc gtggcaatgg tcatcgccgc atcaggaata
      421 tggttcatat ccatatcaat agcgttcagt tcaccacgcg tgcaggaaat ataatcatcg
      481 ccccagcaaa tggtcgcgcc cattttttcc agcacatcag caaagcgaat atcaccctgc
      541 atactgttac gtccaatacc ggtcactttt acagtgccgc ctttgattgc tgctgctgcc
      601 aggaagtaag aagccgaaga tgcatcgcct tcgaccaaat aagtacccgg agactgataa
      661 gactgcccgc cttttacgac aaattgttga tagtgctgat tttcaatttc aacaccaaac
      721 gtcttcatca gattgagtgt gatgtcgata taaggtttag aaaccagatc gcctttaata
      781 cgaatcaccg tatcttccgg cgcaagaggc gcagtcatta acagtgcggt gaggaattgg
      841 ctggaaacgg agccatcaac gtcaacgttg ccgccagtaa agccgccctg taaacgcaac
      901 ggcggataat tttcttgttc caggtaagtg atcttcgccc cgcccaggcg cagcgcatcc
      961 accagatgac caatcgggcg ctctttcata cgcggctcac cggtcagcac aatatcattg
     1021 ctatccagac aaagagctgc cgccagcgga cgcattgccg ttccggcgtt accgaggaac
     1081 aactccaggg caccttctgc gtgtaatgga ccgccgttac cgataatttc gcaacgcgta
     1141 cgatcggctg aaagcgtata gcttaaccct aacgctgtta atgcattcag catatggcgc
     1201 acgtcgtcgc tatccagcag attggttaat actgttttgc cgtgtgctaa tgccgcaagc
     1261 aataaagcgc ggttagaaac gctcttggaa ccgggcagat taatagtgcc atcgacacga
     1321 gcgatgggtt gtaacgtcag ggattccatg aactcaactc tctacaacag aaataaaaac
     1381 cccacaggct ggctgtgggg attaagcaaa atttcggcat taaccgtgac ggcgttcgaa
     1441 ctcaaccatg aagtctgtca gcgctttaac gccttccagc ggcatggcat tataaataga
     1501 cgcgcgcatt ccgccgacca cacggtgtcc tttcagtgca tgaaggcctg cagcaaaaga
     1561 ctcttcaagg aacaatttgt caagcgcact gtccgccaac tggaacggca cgttcatccg
     1621 cgaacggtta gctttcgcca catcattgcg gtagaaatcg ctgttatcaa tcaccccata
     1681 tagcagttct gctttttgct gattgatttt atccatttca gctacaccgc cgttcgcttt
     1741 cagccattta aagaccagac cagatagata ccaggcaaat gtcggcggcg tgttaaacat
     1801 ggagccgtta tcgttgagga tggaataatc cagaatcgac ggacacgcga tattcgcttt
     1861 gcccagcaaa tcttcacgaa cgatgacgat tgtcaggcca gccgggccga tatttttctg
     1921 cgcgccagcg taaattacac cataacggct gacgtcaatc ggacgggaaa gaatggttga
     1981 agagaagtcg gcggcgacca ccacatcttt gccgaagtct ggcgtctcgt cgatggcgat
     2041 accgtcgatg gtttcattcg ggcagtaatg catataagcc gcattatcag agagttgcca
     2101 ttcacgcatt ggcttaaccg cgcgcagacc atcaacagtc actttggcgt caaagacatt
     2161 aggcgtgcag tattttttcg cttctttaat ggcacttgcc gcccagtaac cggcatcaac
     2221 ataatctgcg gtggttttat caccgagaat attcagcggt accgcagcaa actgaccgcg
     2281 accaccgcca tggcagaata ataccttgta gttggagggg acattaagaa gatcgcgaaa
     2341 atccttctcg gcttcctctg caacctgaat gaactctttg ccacggtgac tcacttccat
     2401 caccgacgta ccaagaccgt tccagtcgcg cagttcctgt tgagcctgtt taagcacctc
     2461 tgccggtagc attgccggac cagaactaaa attgaagatt tgagccattt cccctcacca
     2521 cgttgcgttg ctattaaccc gcggtcaatc gattgcgacc acaggtaata cattattcct
     2581 gtggctatcg gttttatcat tcagtgacac acatcgcaat gagtaaaacc gttgagagca
     2641 aaaatgtggc ttgcatcaca caaaagaatc tctcggatta ttagcaaaaa gccgatgttt
     2701 ttactgtcag gtcacccgtt ttgcccgact tgccttcgtc cattctggat ttgcacagcg
     2761 cggacataat tgtttttccc ccgcgttcat atgaatgatt tcactacaac gaatgcaggc
     2821 atagtccgcc gactccggca ccactttgta acgctgcgta caatcactgg gtaaaataag
     2881 taaacccggt ttcaccttgt catcttcaaa gaaatagaca ctgatttgcc cgttggtttc
     2941 gagaatcgcc agacgtacct gccccagctg ctccacgcca cgcaatcgta gctccattaa
     3001 agaactcaaa ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatcac
     3061 attaccaggc ggtataacaa cgccagagtg ataaagacga taagtaccgg caccatcggc
     3121 acatcatcat aaaacgccac atctcccgcc gctgaaccca gcgtgagaat gattaacact
     3181 tcaaacagcg acatctgtcg cacaccgcgt cttccggtca ttttgaggaa caaaaaaacc
     3241 agtacaaagg tataaagact acgtagtgca acttcgccaa ggaaatcaaa aggcacttta
     3301 tcaaatgcca tacggtggag atcgaacgcc ttcattttta tgctcttaac gtggtttatc
     3361 cgctaaaagc atagtcaaca gttcatcggc atggcggcgg tgatagcgcc tttgagataa
     3421 aacccgtatt attgcgcgct ttccgtacga ctaaagtgat tttcatgacg caaacattta
     3481 tccccggcaa agatgccgct ctggaagatt ccatcgctcg cttccagcaa aaactttcag
     3541 acctcggctt tcagattgaa gaggcctcct ggctgaatcc cgtgcctaac gtctggtctg
     3601 tacatattcg cgacaaagag tgcgcactgt gttttaccaa cggtaaaggc gcaaccaaga
     3661 aagcggcgct ggcttctgca ctcggtgaat atttcgagcg tctctcaacc aactactttt
     3721 ttgctgattt ctggctgggc gaaaccatcg ccaacggtcc atttgtgcat tatcccaacg
     3781 aaaaatggtt cccactgagc gaaaatgacg atgtgccaga agggctgctc gatgaccgtc
     3841 tgcgcgcatt ttacgatccg gagaatgaat tgaccggcag tatgctgatt gacctacaat
     3901 ccggtaacga agatcgtggt atttgcggtc tgccgtttac gcgccagtcc gacaatcaga
     3961 ccgtttatat tccgatgaat atcattggta acctgtacgt ttctaacggt atgtccgctg
     4021 gcaatacccg caacgaagca cgcgttcagg ggttgtccga agttttcgaa cgctacgtga
     4081 aaaaccgcat tattgctgaa agcatcagct tgccggaaat cccggcagac gtgctggcgc
     4141 gttacccagc ggtggttgaa gcgatcgaaa cactggaaac agaaggtttc ccgatcttcg
     4201 catatgatgg ttcccttggc ggccagtatc cggtgatttg cgtggtactg tttaatcctg
     4261 caaacggcac ctgctttgcc tctttcggtg cgcatcctga ttttggcgta gcactggaac
     4321 gtaccgtgac cgagctgctg caaggtcgtg gcctgaaaga tttggatgtg tttactccgc
     4381 caaccttcga tgatgaagaa gtcgctgaac ataccaacct cgaaacgcac tttatcgatt
     4441 ccagcggttt aatctcctgg gacctgttca agcaggatgc cgattatccg tttgtggact
     4501 ggaatttctc cggcaccacg gaagaagagt ttgctacgct gatggctatc ttcaacaaag
     4561 aagataaaga agtttatatt gccgattacg agcatctggg cgtttatgct tgccgtatta
     4621 tcgtgcctgg catgtccgat atttatccgg ctgaagatct gtggctcgcg aataacagta
     4681 tgggcagcca tttacgtgaa acgattcttt cgctaccagg cagcgagtgg gaaaaagaag
     4741 attacctgaa cctcatcgag caactggatg aagaaggttt tgatgacttt acccgcgtgc
     4801 gtgagctgtt gggtctggcg accgggtcgg ataacggttg gtacaccctg cgtatcggtg
     4861 aattaaaagc catgctggcg ctggctggtg gcgatctgga acaggctctg gtctggaccg
     4921 aatggacgat ggagtttaac tcatcagtat ttagtccgga acgcgccaac tattatcgct
     4981 gcctgcaaac gttgttatta ctggcacagg aagaagatcg ccagccgctg caatatctga
     5041 atgcgtttgt tcgcatgtac ggcgcagatg ccgtagaagc cgccagtgcg gcaatgagcg
     5101 gcgaagcggc gttttacggc ctgcaaccgg tagatagcga tctgcacgcg tttgctgcac
     5161 atcagtcgtt gttgaaggcc tacgaaaagc tgcaacgcgc caaagcagca ttctgggcaa
     5221 aataaaatca aatagcctac gcaatgtagg cttaatgatt agtctgagtt atattacggg
     5281 gcgttttttt aatgccccgc tttacatata tttgcattaa taaaataatt gtaattataa
     5341 ggttaaatat cggtaatttg tatttaataa atacgatcga tattgttact ttattcgcct
     5401 gatgctccct tttaattaac tgttttagcg gaggatgcgg aaaaaattca actcatttgt
     5461 taatttttaa aatttatttt tatttggata atcaaatagt tactccgtat ttgcataaaa
     5521 accatgcgag ttacgggcct ataagccagg cgagatatga tctatatcaa tttctcatct
     5581 ataatgcttt gttagtatct cgtcgccgac ttaataaaga gagagttagt gtgaaagctg
     5641 acaacccttt tgatctttta cttcctgctg caatggccaa agtggccgaa gaggcgggtg
     5701 tctataaagc aacgaaacat ccgcttaaga ctttctatct ggcgattacc gccggtgttt
     5761 tcatctcaat cgcattcgtc ttctatatca cagcaaccac tggcacaggc acaatgccct
     5821 tcggcatggc aaaactggtt ggcggcattt gcttctctct ggggctgatt ctttgtgttg
     5881 tctgcggagc cgatctcttt acttccacag tgttgattgt tgttgctaag gcgagtgggc
     5941 gcatcacctg gggtcagttg gctaaaaact ggctaaatgt ctattttggc aatctggtcg
     6001 gcgcactgct gtttgtactt ttaatgtggc tttccggcga gtatatgacc gcaaatggtc
     6061 aatggggact aaacgtccta caaaccgccg accacaaagt gcaccatact tttattgagg
     6121 ccgtctgtct tggtatcctg gcaaacctga tggtatgtct ggcagtatgg atgagttatt
     6181 ctggccgcag cctgatggac aaagcgttca ttatggtgct gccggtcgcg atgtttgttg
     6241 ccagcggttt tgagcacagt atcgcaaaca tgtttatgat cccgatgggt attgtaatcc
     6301 gcgacttcgc atctccggaa ttctggaccg ctgtcggttc tgcaccggaa aatttttctc
     6361 acctgaccgt gatgaacttc atcactgata acctgatccc ggttacgatc ggtaatatta
     6421 tcggcggtgg tttgttggtt gggttgacat actgggtcat ttacctgcgt gaaaacgatc
     6481 accattaatg gttgtcgaag tacgcagtaa ataaaaaatc cacttaagaa ggtaggtgtt
     6541 acatgtccga gcttaatgaa aagttagcca cagcctggga aggttttacc aaaggtgact
     6601 ggcagaatga agtaaacgtc cgtgacttca ttcagaaaaa ctacactccg tacgagggtg
     6661 acgagtcctt cctggctggc gctactgaag cgaccaccac cctgtgggac aaagtaatgg
     6721 aaggcgttaa actggaaaac cgcactcacg cgccagttga ttttgacacc gctgttgctt
     6781 ccaccatcac ctctcacgac gctggctaca tcaataaagc gttggaaaaa gttgttggtc
     6841 tgcagactga agctccgctg aaacgtgctc ttatcccgtt cggtggtatc aaaatgatcg
     6901 aaggttcctg caaagcgtac aaccgcgaac tggacccgat gatcaaaaaa atcttcactg
     6961 aataccgtaa aactcacaac cagggcgtat tcgacgttta cactccggac atcctgcgtt
     7021 gccgtaaatc cggtgttctg accggtctgc cagatgctta tggccgtggc cgtatcatcg
     7081 gtgactaccg tcgcgttgcg ctgtacggta tcgactacct gatgaaagac aaatacgctc
     7141 agttcacctc tctgcaggct gatctggaaa acggcgtaaa cctggaacag actatccgtc
     7201 tgcgcgaaga aatcgctgaa cagcaccgcg ctctgggtca gatgaaagaa atggctgcga
     7261 aatacggcta cgacatctct ggtccggcaa ccaacgctca ggaagctatc cagtggactt
     7321 acttcggcta cctggctgct gttaagtctc agaacggtgc tgcaatgtcc ttcggtcgta
     7381 cctccacctt cctggatgtg tacatcgaac gtgacctgaa agctggcaag atcaccgaac
     7441 aagaagcgca ggaaatggtt gaccacctgg tcatgaaact gcgtatggtt cgcttcctgc
     7501 gtactccgga atacgatgaa ctgttctctg gcgacccaat ctgggcaacc gaatctatcg
     7561 gtggtatggg cctcgatggt cgtaccctgg ttaccaaaaa cagcttccgt ttcctgaaca
     7621 ccctgtacac tatgggtccg tctccggaac cgaacatgac cattctgtgg tctgaaaaac
     7681 tgccgctgaa cttcaagaaa ttcgccgcta aagtgtccat cgacacctct tctctgcagt
     7741 atgagaacga tgacctgatg cgtccggact tcaacaacga tgactacgct atcgcttgct
     7801 gcgtaagccc gatgatcgtt ggtaaacaaa tgcagttctt cggtgcgcgt gcaaacctgg
     7861 cgaaaaccat gctgtacgca atcaacggcg gcgttgacga aaaactgaaa atgcaggttg
     7921 gtccgaagtc tgaaccgatc aaaggcgatc tcctgagcta cgatgaagtg atggagcgca
     7981 tggatcactt catggactgg ctggctaaac agtacatcac tgcactgaac atcatccact
     8041 acatgcacga caagtacagc tacgaagcct ctctgatggc gctgcacgac cgtgacgtta
     8101 tccgcaccat ggcgtgtggt atcgctggtc tgtccgttgc tgctgactcc ctgtctgcaa
     8161 tcaaatatgc gaaagttaaa ccgattcgtg acgaagacgg tctggctatc gacttcgaaa
     8221 tcgaaggcga atacccgcag tttggtaaca atgatccgcg tgtagatgac ctggctgttg
     8281 acctggtaga acgtttcatg aagaaaattc agaaactgca cacctaccgt gacgctatcc
     8341 cgactcagtc tgttctgacc atcacttcta acgttgtgta tggtaagaaa actggtaaca
     8401 ccccagacgg tcgtcgtgct ggcgcgccgt tcggaccggg tgctaacccg atgcacggtc
     8461 gtgaccagaa aggtgctgta gcgtctctga cttccgttgc taaactgccg tttgcttacg
     8521 ctaaagatgg tatctcctac accttctcta tcgttccgaa cgcactgggt aaagacgacg
     8581 aagttcgtaa gaccaacctg gctggtctga tggatggtta cttccaccac gaagcatcca
     8641 tcgaaggtgg tcagcacctg aacgttaacg tgatgaaccg tgaaatgctg ctcgacgcga
     8701 tggaaaaccc ggaaaaatat ccgcagctga ccatccgtgt atctggctac gcagtacgtt
     8761 tcaactcgct gactaaagaa cagcagcagg acgttattac tcgtaccttc actcaatcta
     8821 tgtaattaga tttgactgaa atcgtacagt aaaaagcgta caataaaggc tccacgaaag
     8881 tggggccttt tttagcacga gagccttttt tgtcagctat ctatacttta aggtgactgc
     8941 caaaacagac tcgacgtagc cttcgagctg cgcaccaaca cggcctcaga tgggccacat
     9001 ctggagaaac accgcaatgt cagttattgg tcgcattcac tcctttgaat cctgtggaac
     9061 cgtagacggc ccaggtattc gctttatcac ctttttccag ggctgcctga tgcgctgcct
     9121 gtattgtcat aaccgcgaca cctgggacac gcatggcggt aaagaagtta ccgttgaaga
     9181 tttgatgaag gaagtggtga cctatcgcca ctttatgaac gcttccggcg gcggcgttac
     9241 cgcatccggc ggtgaagcaa tcctgcaagc tgagtttgtt cgtgactggt tccgcgcctg
     9301 taaaaaagaa ggcattcata cctgtctgga taccaacggt tttgttcgtc gttacgatcc
     9361 ggtgattgat gaactgctgg aagtaaccga cctggtaatg ctcgatctca aacagatgaa
     9421 cgacgagatc caccaaaatc tggttggagt ttccaaccac cgcacgctgg agttcgctaa
     9481 atatctggcg aacaaaaatg tgaaggtgtg gatccgctac gttgttgtcc caggctggtc
     9541 tgacgatgac gattcggcgc atcgcctcgg tgaatttacc cgtgatatgg gtaacgttga
     9601 gaaaatcgaa cttctcccct accacgaact gggcaaacac aaatgggtgg cgatgggtga
     9661 agaatacaaa ctcgatggtg ttaaaccacc gaagaaagag accatggaac gcgtgaaagg
     9721 cattcttgag cagtacggtc ataaggtaat gttctaattt ccccgtaaag cggcaacttt
     9781 attgagttgc cgctttttta ttccgcgctt cgttctgcta cagcgttttt aacacatctt
     9841 atagtgcatc taccatatcc cgtacctgag ataataacga ctgataatag ctcgcgtgaa
     9901 gttcttcccc atcgaatacc actgtattac acaagaaagt gacatcggca tctt
//
LOCUS       DAJVQH010000133         9687 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJVQH010000133 DAJVQH010000000
VERSION     DAJVQH010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.133"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..580
                     /locus_tag="OQM87_003475"
     CDS             <1..580
                     /locus_tag="OQM87_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-2-oxo-heptane-1,7-dioate aldolase"
                     /protein_id="HCS1509479.1"
                     /translation="PVQIKQLLDVGTQTLLVPMVQNADEARKAVRATRYPPAGIRGVG
                     SALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQILDVEGVDGVFIGPADLS
                     ADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQLAKRYLELGALFVAVGVD
                     TTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            602..1957
                     /gene="hpaX"
                     /locus_tag="OQM87_003476"
     CDS             602..1957
                     /gene="hpaX"
                     /locus_tag="OQM87_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCS1509480.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            1967..2857
                     /gene="hpaA"
                     /locus_tag="OQM87_003477"
     CDS             1967..2857
                     /gene="hpaA"
                     /locus_tag="OQM87_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCS1509481.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            2884..3821
                     /locus_tag="OQM87_003478"
                     /pseudo
     CDS             2884..3821
                     /locus_tag="OQM87_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4048..5606
                     /gene="hpaB"
                     /locus_tag="OQM87_003479"
                     /pseudo
     CDS             4048..5606
                     /gene="hpaB"
                     /locus_tag="OQM87_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5624..6136
                     /locus_tag="OQM87_003480"
     CDS             5624..6136
                     /locus_tag="OQM87_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCS1509482.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6529..8679
                     /gene="btsT"
                     /locus_tag="OQM87_003481"
     CDS             6529..8679
                     /gene="btsT"
                     /locus_tag="OQM87_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCS1509483.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            8729..8932
                     /locus_tag="OQM87_003482"
     CDS             8729..8932
                     /locus_tag="OQM87_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1509484.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            8943..>9687
                     /gene="yjiA"
                     /locus_tag="OQM87_003483"
     CDS             8943..>9687
                     /gene="yjiA"
                     /locus_tag="OQM87_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCS1509485.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLL"
BASE COUNT         2302 a         2532 c         2477 g         2376 t
ORIGIN      
        1 tccggtgcaa atcaaacaac tgctggacgt aggcacacaa accttactgg tgccgatggt
       61 acaaaacgcc gacgaagccc gtaaagcggt acgcgccacc cgttatcccc ccgccggtat
      121 tcgcggtgtg ggcagtgcgc tggctcgcgc ctcgcgctgg aatcgcattc ctgattacct
      181 gcaaaaagcc aacgatcaaa tgtgcgtgct ggtgcagatc gaaacgcgtg aggcaatgaa
      241 gaacttaccg cagattctgg acgtggaagg ggtcgacggc gtgtttatcg gcccggcgga
      301 tctgagcgcc gatatgggtt atgccggtaa tccgcagcac ccggaagtac aggccgccat
      361 tgagcaggcg atcgtgcaga tccgcgaagc gggcaaagcg ccggggatcc tgatcgccaa
      421 tgagcaactg gcaaaacgct atctggaact gggcgcgctg tttgtcgccg tcggcgttga
      481 caccaccctg ctcgcccgcg ccgccgaagc gctggcagca cggtttggta atagttcttc
      541 tatttcgatg gcgaagcaaa acaacaactc tgtctactaa acgtacgatg tggagcgcat
      601 catgacgacc tctaccctgc aatataaaga taataaaact gttgaaattg aacatcgtgt
      661 cattaataaa ttatttcgtc gcctaatcat ttttcttttt attctgtttg ttttttcgtt
      721 tcttgatcgc atcaatatag gatttgccgg actgacaatg ggcaaggatc ttggcctgac
      781 gtccacaatg tttggcctcg ccgcgacact cttttatgtc acgtacgtca tgtgcgggat
      841 ccccagtaat atgatgttag cgaaaattgg cgctcgtcga tggatcgctg ggatcatggt
      901 ggtctgggga attgcttcca cctgcacaat gtttgcaaca agccccgaaa cactctatct
      961 actgcgtatg ctggttggta ttgctgaggc gggtttttta cctggtattt tggtttatct
     1021 gacctggtgg ttcccggcat atcatcgggc cagagccaat gccttattta tgatagcgat
     1081 gccagtcact atgatgctgg gattgatcct ttcaggctac attctggcct tggatggact
     1141 gtggaatcta aaaggctggc aatggctctt tttgctggaa ggtctgccat cggtcattct
     1201 tggtgtcgtc acctggtttt atctcaacga cacaccagac caggcgacct ggcttgatga
     1261 aaacgaaaaa caggctttaa aaacgatgat tctccgtgaa caagagagtg catttgccca
     1321 tgcatctgtt ccacgctcaa ccttacgtga agtcctgacc ccagccatca tgctatatac
     1381 acttgcttat ttttgcctga ctaatactct gagtgcgatt aatatctgga cgccgcaaat
     1441 cctacagagt ttcaatactg gcagtagcaa catcatgatt ggtttgctgg cggccatccc
     1501 ccaattctgc acaatttttg gcatgatctg gtggagtcgt cgttccgacc gtcttaaaga
     1561 acgcaaaaag catacgattc ttccctacct gtttgccgct gccggatggt tactcgcttc
     1621 tgcgacagat cacagcctca ttcaactgag cggcatcatt atggcctcaa tgggatcatt
     1681 taccgcgatg actatctttt ggacaacaca ggatcaagtt ataagcttgc aatcgcgtgc
     1741 cgttgcgatg gctgtcatca atgctgtcgg caatgtaggt tcagcagtca gtccattact
     1801 tatcggtatt ttgaaagatg caaccggcag ttttagctca ggtttgtggt ttgtagcagg
     1861 attgctggta ctgggcgcgt tagtcatgac ccgaatccct atggggagtc gggaacactc
     1921 tctttcatct gaacttatcg caccaaaacc acaataagga gtcatgatgt ccagtgaagc
     1981 tatctttgaa catggttatg aaaatattga tttggtccgg gaatatgatg cccgctacgc
     2041 tggtgaagat gtgcattatg aaacatttac ccggttagcg aatttctttg gacgcgatat
     2101 gcgccctcac tggcacgata gatattttca actccattat ctggtcactg gacgcattac
     2161 tttacaactg gatgagcact tctattctgt gcgcgcaccg ctttttgtac taacaccacc
     2221 atcggtgcca catactttct ttactgatcc tgatagcgat gggcatgttc tgaccgtacg
     2281 tcaggagtta atttggcccc tggtagaaaa gctctggcct ggtagtggag atgccgcaat
     2341 gctacaaggt atctgtctgt caccggaggg aatggaacat acactggcgg cattaaatca
     2401 ttattggccg ataatatccc tggagtttca acaaaataag ccaggacggg aaattctatt
     2461 gcaatcactg gcgcaaagta tttttacaat cctactgcgt gaagcaccac caaatgatat
     2521 ttcaacatgc agcgtgcgcg gcgagatgtg tttattccag aagtttaatc ggctgattga
     2581 cgaaaattat cgccaacatt taatggtccc tgaatatgca acaatgctcg gtatgagtga
     2641 atcacggctt accgagctgt gccgacgttt tgccaatcaa tcgccgaaga gattgatttt
     2701 cgaacgagtg agccgggaag ccagacggct tctgctctat agcgagcaaa gcatcaacca
     2761 gatagccctt gatcttggct acaaagatcc cgcctatttc gcccgttttt tcaaccgaat
     2821 gatgggttgc tcaccaacac aattccgtgg tcgctgacca tttatcaacc tgagagactc
     2881 gacatgaaaa tatctttacg ctcactattt gtggcgtgcc tgattgcgcc agtggcattc
     2941 acctacgcct ccgaaccgca ataaatgaaa attgtactac ttggaacgaa aggtggccct
     3001 tctttattaa aagctgaccg tttaccgatg gcaacggcac ttattattgg caataaaacc
     3061 tggttaattg atgctggtta cggggccagc ttacaactgg taaaaaataa tattccactg
     3121 cgccagattg ataatatttt actcacccat ttacacagtg atcatattct ggactatcca
     3181 tcagtcttaa tgaatgcatg ggctagcggt ctgcaggaca aaactattca tgtattcggt
     3241 ccgccaggca cccgagccat gaccgctgcc agttggcagg tcttcgatcg ggacatcaca
     3301 ctgcgtatgg aagaagaagg aaaacccgat cctcgcaaac tcgttacagc tcaagatgtt
     3361 ggtgaaggcg tagtttataa agatgatacc gtgaccgtga ctgcattaaa ggtccctcat
     3421 tctcctttcc ctgacggtga ggcttttgct tatcgcttcg acacacaagg caagcgaatt
     3481 gtcttctcag gagatacctc gtactttccc ccactggcaa ctttctcaga aggcgctgat
     3541 atacttgtgc atgaggccgt acatgagccc tccgtcgcta agctggcaga cagtattggc
     3601 aatggtaaaa cactggcaaa agccattgct tctcaccata cgactattga ggatgtaggc
     3661 aaaattgcca atgaagcaaa agtgaaaaaa ctcatcctta gccacctggt accaatcacc
     3721 gttccggatg aaacatggaa gcaagaagca cagaaaacta ttcagtccct atcgtcgttg
     3781 gtcatgaaaa tatgacgatt accattccat tcgccccata aaaaattccg tccccgttag
     3841 cccttgttag cggggaatat tatgatttgc cttactgtaa gttgatcacg atcgcagttt
     3901 actatttaac tccaccttgc tgtcgaaaag taccaacgtt cgcctgaacc gtctcttccc
     3961 gacaaccgcg ttcaacgctt caattaaata acaaaaacaa cacacaaaat taacaatcgt
     4021 attccgatta atactgtaga ggtcgacatg aaaccagaag atttccgcgc cagtacccaa
     4081 cgtcctttca ccggggaaga gtatctgaaa agcctgcagg atggtcgcga gatctatatc
     4141 tatggcgagc gagtgaaaga cgtcaccact catccggcat ttcgtaatgc ggcagcgtct
     4201 gttgcccaac tgtacgacgc gctgcacaaa ccggagatgc aggactctct gtgttggaac
     4261 accgacaccg gcagcggcgg ctatacccat aaattcttcc gcgtggcgaa aagtgccgac
     4321 gacctgcgcc agcaacgcga cgccatcgct gaatggtcac gcctgagcta tggctggatg
     4381 ggccgtaccc cagactacaa agccgctttc ggttgcgcac tgggcgcgaa tccgggcttt
     4441 tacggtcagt tcgagcagaa cgcccgtaac tggtataccc gtattcagga aactggcctc
     4501 tactttaacc acgcgattgt taacccaccg atcgtcattt gccgaccgat aaagtgaaag
     4561 acgtttacat caagctggaa aaagagactg acgccgggat tatcgtcagc ggtgcgaaag
     4621 tggttgccac caactcggcg ctgactcact acaacatgat tggcttcggc tcggcacaag
     4681 tgatgggcga aaacccggac ttcgcactga tgttcgttgc gccaatggat gccgatggcg
     4741 tgaaattaat ctcccgcgcc tcttatgaga tggtcgcggg tgctaccggc tcgccatacg
     4801 actacccgct ctccagccgc ttcgatgaga acgatgcgat tctggtgatg gataacgtac
     4861 tgatcccatg ggaaaacgtg ctgatctacc gcgattttga tcgctgccgt cgctggacga
     4921 tggaaggcgg tttcgcccgt atgtatccgc tgcaagcctg tgtgcgcctg gcagtgaaac
     4981 tcgacttcat tacggcactg ctgaaaaaat cactcgaatg taccggcacc ctggagttcc
     5041 gtggtgtgca ggccgatctc ggtgaagtgg tggcgtggcg caacaccttc tgggcattga
     5101 gtgactcgat gtgttcagaa gcgacgccgt gggtcaacgg ggcttattta ccggatcatg
     5161 ccgcactgca aacctatcgc gtactggcac caatggccta cgcgaagatc aaaaacatta
     5221 tagaacgcaa cgttaccagt ggcctgatct acctcccttc cagtgcccgt gacctgaaca
     5281 atccgcagat cgaccagtat ctggcgaagt atgtgcgcgg ttcgaacggt atggatcacg
     5341 tccagcgcat caagatcctc aaactgatgt gggacgccat tggcagcgaa tttggtggtc
     5401 gtcacgagct gtatgaaatc aactactccg gtagccagga tgagattcgc ctgcaatgtc
     5461 tgcgccaggc acaaagctcc ggcaatatgg acaagatgat ggcgatggtt gatcgctgcc
     5521 tgtcggaata cgaccagaac ggctggactg tgccgcacct gcacaacaac gacgatatca
     5581 acatgctgga taagctgctg aaataacgca gcaggaggtt aagatgcaat tagatgaaca
     5641 acgcctgcgc tttcatgacg cgatggtcag cctgtcggca gcggtaaata ttatcaccac
     5701 cgagggcgac gccggacaat gcgggattac ggcaacggct gtctgctcgg tcacggatac
     5761 accaccgtcg ctgatggtgt gcattaacgc caacagtgcg atgaacccgg tttttcaggg
     5821 caacggcaag ttgtgcgtca acgtcctcaa ccatgagcag gaactgatgg cgcgccactt
     5881 cgcgggcatg acaggcatgg cgatggaaga gcgttttagc ctctcatgct ggcaaaaagg
     5941 tccgctggcg cagccggtgc taaaaggttc gctggccagt cttgaaggag agatccgcga
     6001 tgtgcaggcg attggcacac atctggtgta tctggtggag attaaaaaca tcatcctcag
     6061 tgcagaaggt cacggactta tttactttaa acgccgtttc catccggtga tgctggaaat
     6121 ggaagctgcg atttaagctc cagccccttc acgcattcgt cgaaggggca tttttattgt
     6181 gatctcaatc acatatctac ccgatgaata attcttgccc aaccgctcac cgccattcct
     6241 gaccgcttac atccctaaaa taaccactca gttatttacc ttactttacg cgcacgtatc
     6301 tctggcaaca tcactgcagg atagcggtca atttacctcc tcaaacgcaa cgcaaaccta
     6361 gaacggcttc ggcctatgat taacaaatta ctctatggat tttggtttgc aggaaggcag
     6421 caagtgagtg aatccccggg agcttacaaa agtaagtgtc agaggtgaac gaacgcagct
     6481 gccgcacctg taagccaaaa gacgacgagt aaaatgccag gttttactat ggatacgaaa
     6541 aaactattca agcacatacc ctgggtgatt ctcggaatca tcggtgcatt ctgcctcgcg
     6601 gtagttgcat tacgtcgggg ggagcacgtc agcgccctgt ggatcgtggt cgcctctgtg
     6661 tcggtgtatc tggtggcgta tcgctactac agtctgtaca tcgcccagaa ggtgatgaaa
     6721 ctcgacccca cgcgcgcgac gcctgcggtt attaacaacg acggtctgaa ctacgttccg
     6781 accaaccgtt acgtgttgtt tggtcaccac ttcgccgcta tcgccggtgc tggtccgctg
     6841 gtgggaccgg ttctcgccgc gcagatgggc tacctgcctg gcacgctgtg gctgctggcg
     6901 ggggtagtgc tggccggtgc ggtacaggac tttatggtgc tgtttatctc ctctcgccgt
     6961 aacggcgcat ctctcggtga gatgatcaaa gaagagatgg ggccagtacc ggggactatc
     7021 gcgctgtttg gctgtttctt aatcatgatc atcatcctcg ccgttctggc gctgattgtg
     7081 gttaaagccc tggccgaaag tccgtggggt gtcttcaccg tttgctcaac cgtaccgatt
     7141 gcgctgttta tgggtatcta catgcgcttt atccgtccgg ggcgtgtggg tgaagtctct
     7201 gttattggta tcgtgctgct ggttgcctct atctacttcg gtggcgtgat tgctcacgat
     7261 ccgtactggg gtccggcact gacctttaaa gacaccacca ttaccttcgc gctgattggc
     7321 tatgcgtttg tttccgcact gctgccggtg tggctgatcc tcgcaccgcg cgactatctg
     7381 gcaaccttcc tgaaaatcgg cgttatcgtc ggcctggcgc tgggtatcgt ggtgctgaac
     7441 ccggaactga aaatgcctgc gatgacccag tacattgacg gtactggccc gctgtggaaa
     7501 ggcgctctat tcccgttcct gttcatcacc atcgcctgtg gtgcggtatc tggcttccac
     7561 gcgctgatct cttccggtac gacgccgaaa ctgctggcta acgaaaccga cgcgcgtttc
     7621 attggctacg gcgcaatgct gatggagtcc ttcgtggcga ttatggcgct ggttgcagcg
     7681 tccatcatcg aaccgggtct ttacttcgcg atgaacaccc cgcctgctgg ccttggcatc
     7741 accatgccta acctgcatga aatgggtggc gagaacgcgc cgatcatcat ggcgcagctg
     7801 aaagacgtta ccgcacacgc ggcagcgacc gtcagctcct ggggcttcgt gatttcgcca
     7861 gagcagatcc tgcaaaccgc gaaagacatt ggtgagcctt ctgtcctgaa ccgtgcaggt
     7921 ggtgcgccga cgctggcggt aggtatcgct cacgtgttcc acaaagtgct gccgatggct
     7981 gacatgggct tctggtatca cttcggtatt ctgttcgaag ccctgttcat cctgaccgcg
     8041 ctggatgcgg gtacccgttc tggccgcttt atgctgcaag acctgctggg taacttcatc
     8101 ccgttcctga aaaaaaccga ctctctggtt gccggtatca tcggtactgc gggctgtgtg
     8161 ggtctgtggg gctacctgct gtatcagggc gtggtcgatc cgctgggcgg cgttaagagc
     8221 ctgtggccgc tgttcggtat ctccaaccag atgctggcag ccgtagcgct ggtactgggc
     8281 accgttgtgc tgattaagat gaagcgcacc caatacatct gggtaactgt tgttccggct
     8341 gtatggctgc ttatctgcac cacctgggcg ctgggtctga aactgttcag caccaacccg
     8401 cagatggaag gcttcttcta catggcaagc cagtacaaag agaagattgc taacggtact
     8461 gacctgacgg cgcagcagat tgccaacatg aaccacatcg ttgtgaacaa ctacaccaac
     8521 gcaggcctga gtattctgtt cctgattgtg gtgtacagca tcatcttcta cggtttcaaa
     8581 acctggcttg cggtgcgtaa cagcgacaaa cgtactgaca aagaaacacc gtacgttcca
     8641 atcccggaag gcggcgtgaa gatctcttcg caccactaac cgtgtttagc cccgcttcgg
     8701 cggggctttg ttcttatcag agtgaactat gtttggtaac ttaggacagg caaaaaaata
     8761 tctcggccag gcggcgaaga tgttgattgg tattccagac tacgacaact acgtcgagca
     8821 tatgaagacc aaccatcccg acaagccgta catgagctat gaagaattct tccgcgaacg
     8881 ccagaacgcg cgctacggcg gcgatggtaa aggcggtatg cgctgttgtt aatggagaga
     8941 gcatgaaccc gattgcagtt accctactca ccggtttttt aggcgcgggc aaaactaccc
     9001 tgctgcgcca tattcttaac gaacaacacg gctacaagat tgccgtgatt gaaaacgaat
     9061 tcggcgaagt ctccgttgat gatcaactga ttggcgaccg cgccacgcag atcaaaacgc
     9121 tgaccaacgg ctgcatctgc tgttcgcgct ccaacgagct ggaggacgca ctactcgacc
     9181 tgctggataa tctcgacaag ggcaatattc agttcgatcg tctggtgatt gaatgcaccg
     9241 gcatggccga tcccggcccg attattcaga cctttttctc ccatgagatt ttatgccagc
     9301 gctacctgct ggacggcgtg attgcgctgg tggatgcggt acatgccgat gagcagatga
     9361 accagttcac catcgcccag tcccaggtgg gctacgccga ccgtattctg ctgactaaaa
     9421 ccgacgtcgc aggcgaagca gagaaactgc gtgaacgcct ggcgcgcatc aacgcccgcg
     9481 caccggtcta caccgtcacc cacggcgaca tcgacctggg cctgctgttc aacaccaacg
     9541 gttttatgct ggaagagaac gtcgtcagca ccaaaccgcg tttccacttt atcgcggata
     9601 aacaaaacga tatttcgtca attgtggtgg aactggatta cccggtagat atcagcgaag
     9661 tttcccgcgt gatggaaaac ctgctgc
//
LOCUS       DAJVQH010000134         9513 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJVQH010000134 DAJVQH010000000
VERSION     DAJVQH010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.134"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(437..643)
                     /locus_tag="OQM87_003484"
     CDS             complement(437..643)
                     /locus_tag="OQM87_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCS1509486.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            956..1045
                     /gene="kdpF"
                     /locus_tag="OQM87_003485"
     CDS             956..1045
                     /gene="kdpF"
                     /locus_tag="OQM87_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCS1509487.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1045..2718
                     /gene="kdpA"
                     /locus_tag="OQM87_003486"
                     /pseudo
     CDS             1045..2718
                     /gene="kdpA"
                     /locus_tag="OQM87_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
     gene            2741..4788
                     /gene="kdpB"
                     /locus_tag="OQM87_003487"
                     /pseudo
     CDS             2741..4788
                     /gene="kdpB"
                     /locus_tag="OQM87_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4797..5369
                     /gene="kdpC"
                     /locus_tag="OQM87_003488"
     CDS             4797..5369
                     /gene="kdpC"
                     /locus_tag="OQM87_003488"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCS1509488.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAVAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5362..6345
                     /locus_tag="OQM87_003489"
                     /pseudo
     CDS             5362..6345
                     /locus_tag="OQM87_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001381859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            6345..8041
                     /locus_tag="OQM87_003490"
                     /pseudo
     CDS             6345..8041
                     /locus_tag="OQM87_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141249.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
     gene            8038..8715
                     /gene="kdpE"
                     /locus_tag="OQM87_003491"
     CDS             8038..8715
                     /gene="kdpE"
                     /locus_tag="OQM87_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCS1509489.1"
                     /translation="MTNVLIVEDEQAIRSFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8990..9094
                     /locus_tag="OQM87_003492"
     CDS             8990..9094
                     /locus_tag="OQM87_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCS1509490.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9396..>9513
                     /locus_tag="OQM87_003493"
     CDS             9396..>9513
                     /locus_tag="OQM87_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS1509491.1"
                     /translation="MNKMSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAI"
BASE COUNT         2115 a         2394 c         2636 g         2368 t
ORIGIN      
        1 aacataacat cttattggat tagatatgtt aacaattaat tattcttaat atatcaataa
       61 agtatatatt actctcttta ctatctgttt ttattgcggt agcgatacta ataacaaaat
      121 tcggaattta tttagatgct ggaattagaa aaagggatga cggctttctc accttaccct
      181 gaagtttcac gaaagtgtgt aaggaagtat gcaatctaac tggagatttc tgatgatcta
      241 gtcgcttaga gcgacaatga aggtgaccgc atcctgggta gccctatgaa aggatactat
      301 tgaaacgtga tgctaaaaca gtgatgaaat gaataatacg atggaaacgt cttccatgtt
      361 aattatcatg gaagacgttg atgttgaaca cctaaaccag ccccctgata cgaggctggc
      421 ttaagcgcgc aggcaatcag taaaaatcac cggttgcctt ttcggcctga tccatccaca
      481 ccggtttatc gctggttttc gaccagacgc ggtgcaggta gctgtaaaag cgggcgcggt
      541 ctttccagaa aaacatgaaa ggcagcgcca gaacgcccgc tgcaaccgca tatgtacggc
      601 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      661 gtatctaccc ggtgaatagc accggaaaaa tgaatttgtt tatctgatga aaatagtacc
      721 gccttttgtg taattttacc actcatccga ccacttattt ttgcttattg atggtttatt
      781 tacattcaca ctgtaattaa attacacaaa agttaaatta atactaacca tcagttaaat
      841 catggccttt gccattttta tacttttttt acaccccgtc cgcagatttt tgcgaaatct
      901 ttgcagccag aattctactc ttccggtatc acttttaggc tactggaggt gcactgtgag
      961 tgcaggcgtg atagccggcg tgttgctggt gtttttatta ctgggttatt tggtttatgc
     1021 cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat tgccacgttt
     1081 ttactggtat taatggtgct ggcgcgtcct ttaggcagcg ggctggcgcg gctgattaat
     1141 gacattcctc ttccaggtac agcgggcgtt gaacgtatac tctttcgttt acctggcgtc
     1201 tctgaccatg agatgaactg gaagcaatat ctttgtgcca ttctcggcct gaacatgctg
     1261 gggctggcgg tgctgttttt tatgttgctc ggtcagcact atctgccgct taatccatag
     1321 cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg ccgtcagctt tgtcacgaat
     1381 accaactggc aatcttatag cggtgaaacc acgttgagct atttcagcca gatggcaggc
     1441 ttaacggtac aaaactttct ttctgccgcc agcgggattg cggtgatttt tgccttcatc
     1501 cgtgcgttta ctcgccagag catgagcaca ctcgggaatg cctgggtcga tctgctacgc
     1561 atcacgttat gggtgctagt ccctgtggcg ttgttaatcg cactgttttt tattcaacaa
     1621 ggtgcgctgc aaaactttct gccctatcag gcagtgaata ccgttgaagg agcgcaacag
     1681 ctgttaccca tgggaccagt agcttctcag gaagcgatca agatgctcgg tactaacggc
     1741 ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa acccaaccgc actgaccaac
     1801 ttcgtgcaga tgctggcgat cttcttgatc ccaacagcgc tgtgctttgc ctttggtgaa
     1861 gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg cgatgtcagt gatttttgtc
     1921 atctgcgtag gcgtggttat gtgggcagaa gttcagggta atcctcatct gctggcgctc
     1981 ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc gtttcggcgt gctggtcagt
     2041 agcctgtttg cggtcgtgac tacggcggcg tcctgtggcg cggtgattgc gatgcatgat
     2101 tcgtttaccg ctctcggcgg catggtgccg atgtggctaa tgcaaattgg tgaagtggtg
     2161 ttcggcggtg tcggttctgg tctttacggc atgatgctgt ttgtcctgct ggcggtgttt
     2221 attgccgggc tgatgattgg tcgtacaccg gaatatctgg gtaaaaaaat cgacgtacgc
     2281 gagatgaaac tgactgcact ggcgattctg gttactccaa cactggtact gatgggcgcg
     2341 gcgttggcga tgatgaccga cgccggacgt agcgccatac ttaaccctag cccgcatggt
     2401 tttagcgaag tgctgtacgc cgtgtcgtcc gccgctaaca acaacggcag cgcctttgcc
     2461 ggattaagcg ccaactctcc gttctggaac tgtttactgg cgttctgcat gtttgtcggt
     2521 cgctccgggg tgattatccc ggtgatggca attgccggtt cgctggtgag taaaaagagc
     2581 caacccgcca gctccggcac gctgccaacg cacggcccgc tgtttgttgg cctgttaatc
     2641 ggcaccgtgt tgctggttgg cgcactgacc tttatccctg ccctggcgct tggtccggtg
     2701 gcggaatatc tctcctgatg atattgagtg agcactgaat atgagtcgta aacaactggc
     2761 gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa gcggtgaaaa aattaaaccc
     2821 gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg atcggcagtc tgctgaccac
     2881 ctgtattagc atcgcgatgg caagcggtgc gatgcctggc aatgcgctgt ttagcgcggc
     2941 aattagcggc tggctgtggg tcaccgtact gttcgctaat ttcgccgagg cgctggcaga
     3001 aggccgcagt aaagcgcagg ccaacagtct taaaggggtg aaaaaaactg cctttgcccg
     3061 caagctgcgt gagccgaaat atggcgctgc ggcggacaaa gttcctgccg atcaactgcg
     3121 taaaggtgac atcgtgctgg tagaagctgg cgatattatc ccctgcgatg gtgaagtcat
     3181 tgaaggcggc gcatcggtcg atgaaagcgc catcaccggg gaatcggcac cggtgatccg
     3241 tgaatccggc ggcgattttg cctccgtcac cggcggcacg cgtattcttt ctgactggct
     3301 ggtgattgag tgtagcgtta accccggtga gacatttctg gatcggatga tcgcgatggt
     3361 ggaaggcgca cagcgacgca aaacaccgaa cgagattgcc ctgactatcc tgctgattgc
     3421 tctgaccatc gtctttttac tggcaaccgc cacgctgtgg ccgttttccg cgtggggcgg
     3481 taatgccgtg agtgtaacgg tactggtggc gctgctggtc tgtctgatcc caaccactat
     3541 tggtggcctg ttgtcagcga tcggcgtcgc cgggatgagt cggatgctgg gcgcgaatgt
     3601 gattgccacc agcggacgtg cagttgaagc ggcaggtgac gttgacgttc tgctactgga
     3661 taaaaccggc accatcacac tcggtaaccg tcaggcgtcg gagtttatcc ccgcgcaggg
     3721 cgtggatgaa aaaacgctgg ctgacgccgc acaactggct tcgctggctg atgaaacgcc
     3781 ggaaggtcgc agtattgtga tcctcgccaa gcaacgtttt aacctgcgcg agcgcgatgt
     3841 gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa agccggatga gcgggatcaa
     3901 catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc attcgccgcc atattgaggc
     3961 taacggcggt cacttccctg ccgatgttga tcaaaaagtc gatcaggttg cgcgtcaagg
     4021 agccacgccg ctggtggtgg tggaaggttc tcgtgtgctg ggcgttattg cgctgaaaga
     4081 tatcgtcaaa ggcggtatta aagagcgctt cgcccagctg cgcaaatggg cattaaaacg
     4141 gtgatgatta ccggcgataa ccgtctgact gccgccgcga tagctgcgga agcgggtgtc
     4201 gatgattttc tcgccgaagc gacaccggag gccaagctgg cattgatccg tcagtatcag
     4261 gcggaaggtc gtttggtagc gatgaccggc gacagcacca acgatgctcc ggcgctggcg
     4321 caggcagatg tcgcggtggc gatgaactcc ggcacccagg cggcgaaaga ggcgggcaat
     4381 atggtggatc tcgactctaa cccgaccaag ttgatcgagg tggtgcacat tggcaaacag
     4441 atgctgatga cccgtggctc gctgaccacc ttcagcattg ccaacgatgt ggcgaaatac
     4501 ttcaccatta ttccggcggc gttcgcggca acatatccgc agttaaatgc gctgaacatc
     4561 atgcgcctgc attcgcccga ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt
     4621 atcgtctttt tgattcctct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct
     4681 gccatgttgc gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt
     4741 ggtatcaaag tcattgattt actgctaacc gtttgtagtc tggtgtgagg tttaccatga
     4801 gtggattacg tccggcatta tcgacatttc tctttctgtt attgattact ggcggcgttt
     4861 acccgctgct gaccaccgca ctggggcaat ggtggtatcc ctggcaggcc aacggttcgt
     4921 tgattcgtga aggtgatacg gtgcgcggtt cggcattaat cgggcaaaat tttaccggca
     4981 acggctattt tcatggtcgc ccgtcggcaa cagcagaaat gccctataat ccacaggctt
     5041 ctggcgggag caatctggca gtcagtaacc cggagctgga taaacagata gccgcacgcg
     5101 ttgccgcatt acgggccgct aacccggacg ccagcacgaa cgttccggtt gaactggtga
     5161 cggcgtcggc aagcggactg gacaataaca tcaccccgca agcggtggcc tggcagatcc
     5221 cacgcatcgc aaaagcgcgt aatctcagcg ttgaacagct cacgcagctg atcgcaaaat
     5281 acagccaaca accgctggtg aaatatatcg gccagccggt cgtcaacatt gtcgaactca
     5341 atctggcact ggataagctt gatgaataac gaacccttac gtcccgaccc cgaccgtctg
     5401 ctggaacaaa ctgccgcgcc gcatcggggg aagctgaaag ttttcttcag tgcctgtgca
     5461 ggcgtcggga agacctgggc gatgctggca gaagcccagc gactgcgggc gcaagggctg
     5521 gatattgtgg ttggtgtggt agaaacccat gggcggaaag ataccgccgc catgcttgaa
     5581 gggctggcta ttctgcctct gaaacgccag gcgtaccgtg ggcggcatat cagcgagttt
     5641 gatctcgatg ccgccctcgc ccgccgtccg gcgctgatct taatggacga actggcgcac
     5701 agtaatgcgc caggttctcg tcatcctaaa cgctggcagg atatcgaaga actgctggaa
     5761 gctggcattg atgttttcac aaccgtcaac gttcagcatc tggaaagtct gaatgatgtg
     5821 gtcagcggcg tcaccggaat tcaggtacgg gaaaccgtgc ccgatccttt tttcgatgcc
     5881 gccgacgacg tggtgctggt ggacttgccc ccggacgatc tgcgccagcg gctgaaagaa
     5941 ggcaacgtct atattgccgg gcaggcggag cgcgccattg aacatttttt ccgcaaaggc
     6001 aatctgatcg ccctgcgcga actggcactg cgccgtactg ccgatcgcgt tgatgagcaa
     6061 atgcgcgcct ggcgggggca tcctggcgaa gagaaagtct ggcacacgcg cgacgcgatc
     6121 cttttatgca tcggccataa caccggcagc gaaaactggt ccgcgcggcg gcgcggctgg
     6181 cgtcacggct ggggagcgtc tggcacgcgg tgtatgttga accccctgcc ctgcatcgct
     6241 taccggagaa aaaacgtcgg gcaattctca gcgccttacg tctggcgcag gagctgggcg
     6301 cggagacggc aacactttct gatccagcag aagagaaagc ggtagtgcgt tatgcccgtg
     6361 aacataatct cggcaagatt attctcggtc gcccagcctc gcgccgctgg tggcgtcggg
     6421 aaacatttgc tgaccgactg gcgcgcatcg cccccgatct cgatcaggtg ctggtcgcgc
     6481 ttgatgaacc acccgcccgc acgattaaca acgcgccgga tagccgctca tttaaagaca
     6541 agtggcgggt acagattcag ggatgcgtgg ttgccgccgc gttatgcgcc gttatcacct
     6601 taattgccat gcagtggctg atggcgtttg atgccgccaa cctggtgatg ctgtatctgc
     6661 ttggcgtggt ggtggcgcta ttttatggac gctggccttc ggtggttgcc accgtcatta
     6721 atgtggtgag tttcgatctc ttttttatcg ccccacgcgg cacgctcgcc gtctctgatg
     6781 tgcaatatct gctgaccttc gcggtgatgt taaccgtcgg gctggtgatc gggaacctta
     6841 ctgctggcgt gcgttatcag gcgcgggtag cccgttaccg cgagcaacgc acacggcact
     6901 tatatgaaat gtcgaaagct ctggcggtgg gccgcagtcc gcaggacatc gccgccacca
     6961 gcgaacaatt tattgcctct acgtttcatg cccgcagtca ggtgttgttg cccgatgaca
     7021 acggtaaatt gcagccgtta acacatccgc aaggaatgac gccatgggac gatgccatcg
     7081 cgcagtggag ttttgataaa ggcctgcctg cgggcgcggg caccgacacg ttacccggtg
     7141 taccgtacca gattttgccg ctaaaaagcg gcgagaaaac ctacggctgg tggtggtgga
     7201 gccggggaat ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacttttac
     7261 gctgttagtc gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg
     7321 gatggcaagc gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt
     7381 acgcacgccg cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag
     7441 cgaaggatca ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac
     7501 ccgactggtg aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa
     7561 gaaagagtgg ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg
     7621 tttatcgtcg cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg
     7681 gccactcttt gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc
     7741 gcaggccgaa attggtatcg atgcccacgt tgagggcgaa aatctacaac tggatgtctg
     7801 ggataacggc cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg
     7861 cgggaataaa gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat
     7921 tgtggatgta cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt
     7981 tcgtgttaca cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg
     8041 acaaacgttc tgattgttga agatgaacag gctattcgta gctttctgcg cacggcgctg
     8101 gagggcgacg ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg
     8161 gcaacccgta agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt
     8221 gagtttatcc gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc
     8281 agcgaagaga gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag
     8341 ccgtttggca ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc
     8401 accgccgcgc ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc
     8461 gtgattcacc ggggtgagga agaggtgcat ctcacaccaa ttgagttccg cctgctagcg
     8521 gtgctgctca acaatgccgg aaaagtactc acccagcgcc agctccttaa ccaggtgtgg
     8581 gggccaaacg cggtcgaaca cagtcactat ttgcgtattt atatgggaca tctgcgacaa
     8641 aaactggaac aggatccggc ccggcctcgc cacttcatta ctgaaaccgg tattgggtat
     8701 cggtttatgc tttgaattgt tattcaataa attcgcttaa atagtgatta atttattatt
     8761 aaacctgcgc cgaatattat tatttggcag gtttaatttc ttaacgcaaa tataaaacac
     8821 gaaattacaa catttaagta acaaaacagc atctaatatg gcgatattca taattaatga
     8881 tttattatct ggggtatgat cgttttttgt tgatcttctt cacagattat agccatttca
     8941 tggatagaat aactctacct tcaactgaca cagcaagcgg taaaggtaaa tggaaaataa
     9001 taaccgctta atgcctcata taaggcggac aacccatatc atgaagtttg cccatcgtaa
     9061 tagttttgac tttcatttct ttaatgcccg ctagtcttct gactaaaggg caccccaacg
     9121 tacaggtctc cctgacttta agcattacag gttaatacct gtattcctcg gtgctcatat
     9181 actgctaacc cttttaaact ctaaataatt cgagtcgcag cacttgcaac ttgaggtatg
     9241 acgagtatag ccagttaccg ggctggtctg ggttattgca tctgcaaaaa gcaaactact
     9301 gatttattta tcagcggtgg agctttgctt tttttccggc gtgatcgatt tctcctttga
     9361 gaaattgagg acctgctatt acctgaaata aagagatgaa taaaatgtca gaattaaaaa
     9421 ttgccgttag ccgttcttgc ccggattgtt tttccactca tcgtgcatgc gtgaatattg
     9481 acgaaagtaa ttatattgac gttgccgcca tta
//
LOCUS       DAJVQH010000135         9499 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJVQH010000135 DAJVQH010000000
VERSION     DAJVQH010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.135"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..399)
                     /gene="ompG"
                     /locus_tag="OQM87_003494"
     CDS             complement(<1..399)
                     /gene="ompG"
                     /locus_tag="OQM87_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCS1509492.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRW"
     gene            complement(444..1526)
                     /locus_tag="OQM87_003495"
     CDS             complement(444..1526)
                     /locus_tag="OQM87_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1509493.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(1540..2199)
                     /gene="pgmB"
                     /locus_tag="OQM87_003496"
     CDS             complement(1540..2199)
                     /gene="pgmB"
                     /locus_tag="OQM87_003496"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCS1509494.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(2196..4463)
                     /gene="ycjT"
                     /locus_tag="OQM87_003497"
     CDS             complement(2196..4463)
                     /gene="ycjT"
                     /locus_tag="OQM87_003497"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCS1509495.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     ARGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(4460..5066)
                     /locus_tag="OQM87_003498"
                     /pseudo
     CDS             complement(4460..5066)
                     /locus_tag="OQM87_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(5114..5860)
                     /locus_tag="OQM87_003499"
                     /pseudo
     CDS             complement(5114..5860)
                     /locus_tag="OQM87_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
     gene            complement(5891..6733)
                     /locus_tag="OQM87_003500"
     CDS             complement(5891..6733)
                     /locus_tag="OQM87_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCS1509496.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVTWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(6720..7601)
                     /locus_tag="OQM87_003501"
     CDS             complement(6720..7601)
                     /locus_tag="OQM87_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCS1509497.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLLAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7622..8905)
                     /locus_tag="OQM87_003502"
                     /pseudo
     CDS             complement(7622..8905)
                     /locus_tag="OQM87_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(8919..>9499)
                     /gene="ycjM"
                     /locus_tag="OQM87_003503"
     CDS             complement(8919..>9499)
                     /gene="ycjM"
                     /locus_tag="OQM87_003503"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCS1509498.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMDALSRRESSDEERCARFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         2267 a         2647 c         2324 g         2261 t
ORIGIN      
        1 ccagcgctgc atattcgccg tgtctttgcc cggttcatca acgtagtgat aaccataatt
       61 acggaaaccg ccggtcaggc cgaaactgaa atcatcgctt tcgaggaact gataatgcac
      121 ctccagctcc gggcgatcaa accacgttcc acgtttaccc gcgctataat ctaccggccc
      181 ttcctgataa taggccagag caattctcca cggcccgttg gcggcattaa aatagactga
      241 aggctccgcc agcccatcca tatcttcgcc ataaccctcg acgttttcta tttcgtacat
      301 cgcaccgata ttaaagtgcc agtcgttctt ttcctcggcc tgtgcgcagg ccattcccgc
      361 acacatcacc agtgcggtac agggtaataa ctttttcatt attgttcctt gtattatcct
      421 aggggaatgc cttccccctg tatttagcga attgctattt ccgtttctgc atcaaagaaa
      481 tgacatttcg tcatatcaaa atgaatagtg atattttctc ctgcatgata atcatttaac
      541 gcaccagcac ggaccactaa ctcgtgcccc ccaaccgtgg tgtagagcat aaattccgca
      601 ccggttaatt ctgccacgct aattttggcg gaaatgttat tttcctcttg cgcgtccgga
      661 tgaatatctt ccgggcgtat tcccatcacg atgggcttat gcaaactttc ctgtgttttc
      721 agaaccgcta atttctcttc gagaatggtt aatttaagcg tttccgtaac gaatttatcg
      781 ccatcgatcg tgccgcgaat aaaattcatc gctggtgatc caataaatcc ggcaacaaac
      841 atattcgcgg gttggttata aacggttttc ggcgcaccta cttgctgaac aatcccgtct
      901 ttcataatca caatccgcgt cgccatggtc atcgcttcgg tctgatcgtg ggtcacgtag
      961 atcatggtgg tgttcagttt ctgatgcagc ttgctgatct ctgcacgcat ttgtacgcgc
     1021 agcttggcat caaggttaga gagcggttca tccattaaaa acacgcccgc ttcgcgcaca
     1081 atcgcccgcc cgagcgccac acgctggcgt tgcccgccgg aaagcgcgcc cggcttgcgt
     1141 ttcaggtact cacgcaggcc gagaatttgc gccgcccagt tcacccgctc atcaatcacc
     1201 tctttggcga ttttttgcat cttcaggcca aacgccatgt tgtcgtaaac cgtcatatgc
     1261 ggatacaacg cgtagttctg gaacaccatc gcgatattgc gtgctttggc tggaacgtca
     1321 ttcattcgtt tgccgtcgat caacagatcg ccgccgctga tctcctcaag cccggcaatc
     1381 atgcgcaggg tggtcgattt accgcagccc gacgggccga caaatacgat gaactcttta
     1441 tcggcaattt ccaggttgaa gtccttcacc acatgcacct ggttatcgta gattttttga
     1501 atatgttgta acgaaagctg agccatgttg attcctttgc tatacgtttt gccagaaggc
     1561 cgataaccgc gaccaggtca gggagtccgt tgagggcaac agtaattgcg ccccggttaa
     1621 gcccgcaccg atccccaccg agcgcatacc gctggcgtta atggcgtcaa tgcccgcctg
     1681 cgcatcttca atgccgatac atgcctgcgg cggcacgccc agcccggcac aggcagcgag
     1741 aaagatttcc ggatccggtt tcgagttttt aagttgggaa gcatccgcgc agaaggtgaa
     1801 aaactcgcgc agctccagcg ccgctaaaat cgtcggcgca ttcagggaga cggaagccag
     1861 ccctaccgga atctgctgtg cacggagctc tgccagcaaa ttgcgaatac cgggcagcac
     1921 agcattaacc gtcagctcgc gcagtgagtg gacgtaaagc agatttttgc gatacgccag
     1981 ttgcgcccgc tcctgcgggt taaagtcgcc ctctttgccc ccgtgttgca gaatgcgccg
     2041 cagagactca tcgcggctga tcccttttag agattcgtta aactgcgcat caatactgat
     2101 gccaatttca gcggcaatct gctgccacgc ctggaaatgc agatgcgcgg tatcggtgat
     2161 tacaccatcc agatcgaaaa ttaccccttg cagtttcatt catcctcctg atgcgtggta
     2221 gcggtcccat tgaagggcaa aataaaatca cccaaacaga aaacagattc ttcagccacg
     2281 gatataagct gaccgttcaa acgcagtgaa acgggcgcag aagttcgaat cgcaatacgc
     2341 tgcgcgtcga gagtgacctg taattcgcag ccctgccaga acagagggaa agacaactgt
     2401 tgccactgct caggtaacgc cggatttaga tgcaattcac cgtcacgcac gctcaccccg
     2461 gcaaaaccct gaatcgcccc cagccagata gcgccagttg cggcagcatg gataccatca
     2521 tcacaactat gcggatcagc accaagatcg atttcagtcc cctcgcgcca gaactgataa
     2581 ctttgggtca gcaggccaca gcgtgcagca acaatgccgt ggattgcttt acttaatgac
     2641 gagtcgtgaa tagtgcgcgg ttcataaaat tgcagattgg caagacacga tgccgatgag
     2701 aactgctctg gcagcatgta attgagcatc accacatcag cttgtttgag gatctgcatc
     2761 tcgttcactt ctgcgcgtga ataatccagc agaatggttt gcttccccgc cgccgctttg
     2821 tatttcgcca gattaatcgc cggcttagtc ataaacgaat catcctgcgg caaaacaccg
     2881 tcgggctgaa tttctggcag ccgcagttct ttaaggaaca tttcggcacg atgaataaac
     2941 gcgtcgtcac tacagccaaa ctggcgggca atactcagcg cctgctggac gttgtaatac
     3001 accatatagc tggtgaaggc gttattattg acatgttcgg tatattcgtc tggcccaata
     3061 acatcatgaa tttccagacg gtcgttaacc ctcaccgcgc ggctaatcca gaactttgca
     3121 gtttccagaa gtagcgccat gccttcatgc gcaatgaaac tttcatcccc cgtggtctgc
     3181 cagtattgaa taaccgccca ggcgatatcg gccaccagat gatgttccgc ctgcgccgag
     3241 gccacttttt gccgcagccc ggtgcgaatg ttaatggcgg caaattccgg cgtctcttct
     3301 tcgccgctgc gcgcgctttc ccacggaaat agcgcgccct gccagccgtt gcgtcgcgct
     3361 ttctcctgcg cgcctggcaa gttgtgccag cgataacgca gtaaacttcg ggcaaccgtc
     3421 ggatcgctaa acagatggaa cggcagcaaa aatacttctg tatcccagaa aacgtggcct
     3481 ttgtagcctt cgccggttaa gccttttgcc gcaatactgc tgcgctcgcc gtgagccggc
     3541 gtcatgatgc gcagatgata aagcgcataa tctaacgctt gctgatcgtg cgcatcgccg
     3601 ccatttaccg tgatacgacg tttctgccac cattgacgcc agttttctgt tgatgctgca
     3661 agatgttggt cgtaactctg ttgcgcgcac atttcaagct ggcgaagcga cacgctgccc
     3721 cactcgtcta aagcagcttg cctgtcatcc cgccagtcga tccacaccag tttttgcaac
     3781 gtcactgtct cgcctgcatg aagctgcgca ctggtatgtt gcagtaaacg gcgctctttg
     3841 gcggtatagc attgctgcac atcaccgctc acctggcagc aacaactgat ggccacatca
     3901 ctgcgcccat cctgggtggt gtagatcccc tgcatcaaat gctgaccaaa cacccgcacc
     3961 tgggtttcgt cgagatgctg gcgaccgtgg ttggtttgcg tggcgtcaat gcctgttgaa
     4021 atcagcactg acgcgtcggc gtccagtggc ataatagtga tttccagcgc aatgagcggc
     4081 agttggtctg ccgaaacaaa gcgacggctg gcgatggtgt aacctgcgcc gttgctggta
     4141 cgccagacaa cgctacggcg taattctcca ctggcaaagt caagttcacg ctgccaggct
     4201 tcgcgggata acgagaaaac ctcaccattt atggcaatct ccatccccaa gatatcaggc
     4261 agattcacca gttcgttgat ttcacctttt cccgcccgat gatacagccc cgccagatac
     4321 atccctcgcg cctggcgcgt gtaatcttct tcatggctgg cgcgaagccc aagatagccg
     4381 ttcccctgcg ccatcagcga tgcatacttg ttcagggtat gctggctgaa atggggttct
     4441 gataacgtta ctggcctggt cataattcca cacgcgtccc tgtttctgcg gattgatata
     4501 acgccgccac cagttgctgg atgatgtacc cctgctcggc gtcggctatc atcacgggct
     4561 tgccctgtac gtgattgata aaggcttcca tgctgcgcaa atggcggttg tcgtctgcca
     4621 tttcccgttg catcagcgtc attaattcac cgttgttatc ggtgtagata tgtgctggaa
     4681 acagcgtcgc accggcttta tcaccacaaa agctgacgtt cataatcaac tgttcgcgga
     4741 tgttgagtgc aaatgacgtt tccagccaca gaatgccgcc gttatgaaat tcaatggtgc
     4801 caaacagcga atcttcgacg ctgtaagttg ccggatccca ctcgccaaat tgaccacagc
     4861 tcttttgcgt gccgatcttt tgaaaactat gcgcattcac gcttttcacc gccggaaagc
     4921 ccagcacata catcgcagca tccagcatat gaatgccgat gtcgatccag ttctttattg
     4981 gtaaagacac cccagccggg aacgccgcag cgacgcaggg cgcgggcggt ggtaacgtaa
     5041 atctctccca aaacgccgtt ggtcacctgt tcacgcagct gttgcgtatc gagcgcaaac
     5101 gatggtgaaa gtcgtaggcc aggccgcgca gcgccgattg cagcgcgtcg gcgtaaccgc
     5161 tggtttcgat aatcacatcg gccccctgct tgccggtcag cgttttgatc tctttgccta
     5221 cgtcagtgcc aatggggtta aggcagaaat cagcaccgtg gcgacgggca atatcacagc
     5281 gatgggcgat aggatcgacg ccaattacca ccgatgcgcc agcgcgtttc gccagttgga
     5341 tggcaatttg accgatcgcg ccaagcccta ccaccacgac aaaatcccct acgcgtacgt
     5401 tggcatcgcg cacgccgctc atggcaaact gcgccgggtc gtaacagacg gcgtttttcc
     5461 aggagctgcc ttgcggcatt ttgcgcagct tgtagttatt cactgcgtta atgatgaccg
     5521 tctcggagag cgggccgtag ccgcatacgc tgtcgcccac cgcgtagtct gtaacgtcgc
     5581 tgccgcactc gataatgtcg ccaaccacca tgttgccaag ctggaatttg ccaaactcaa
     5641 tgccgcgcgg cgcatctgcc ggacgcgggg tgaacatctg ccactcgccg ttaaagtctt
     5701 catcgataaa cgggctggcg gcgcggaagt cgaccacttc cgttccgtgt ttcggtgcgc
     5761 cgaaacgcac gcggatcttc acttcattgg ctaaaatcgc ccgatcttca tactcaacca
     5821 gcgcagcaac acgcggtgct gtggctacta actttttcat gtaaaactcc ttgtaaagaa
     5881 actggccgct ttagcccttc acgccaccgg cggtcaaacc actcttaata aaacgttccg
     5941 aaagcgcata cataatcacc accggcaatg cggtcaccag cgaggccgcc atcatccgtc
     6001 cccagatgta atcaggcgta ctgaacagcg cgttcaggcc cactggtaag gtgaaattgc
     6061 tggcgctgga gaggaaaatc gacgcaaaca gatagtcgtt ccacgtcacc ataaagcagt
     6121 agacgaacac cgatatcagc ccggacatcg ccaggggcac ggtgatacgg aagatgattt
     6181 gcagacggtt gaggccatcc atcatcgccg cttcttcgat ctcatcaggg atggtgtcga
     6241 agtaactttt cagcataaac acggcggtgg gcagggtttg cgtcaccatg gtaatgatca
     6301 gcgccatctc ggtgtcgtaa ataccaagcg cggtgatgat tttgaacaga ggcaccacca
     6361 gcaaaatgcc agagaacata tacaccgtgt aaaagctggc gttgatggtc atccggcctt
     6421 taaagcgcag gcgggaaagc gcgtaagctc cgagaatgcc gagaaatacc gccaccacgg
     6481 aggaaaccac cgacaccacc atgctgttac ggaagtagtc gacaaacggg aaaatcatcg
     6541 ggttaaaaat gtcgacgtaa tgctccagcg tccactgttg cggcagcagc gtaggatgca
     6601 gcgatatcgc ctctttcgcg cttttgaacg aggtcatcag catcacaaaa aacggaaaca
     6661 gcgtgatgat gagaaacagc gccagcccgc agtaaaaacc gatacgactg agcgtgcgtt
     6721 tatttgttgc cattgaggtt cacccgtttc ctggtcagca gaatgacagc gaaaataatg
     6781 atgaagagca ccaccgagat cgccgccgct ttccctaagt cattaaaggc aaaggcggtt
     6841 ttgtagagat agacaccaag aatatcgacc ttggttgtca gcagataaac atcagcgaac
     6901 atgtagaaca tccagatggt gcgcagggtc accaccgtcg ccaagaccgg cataattgcg
     6961 ggcagcgtga cgatgcgaaa ccgctgccag gcattagcgc catccatctc tgccgcttcg
     7021 tacagcgatt tgtcgatggt ctgcaaaatc gccagaaacg agataaaggc atacgggaag
     7081 tagcgccaga tggcgagcag caccaccagc acaaagctac tgcccggatt atcaaaccac
     7141 agcggtgcct gctcatagag atgcagaaga tcgacgccga ggtagttcac aatgccgtag
     7201 ccgttgttga acatgtattt ccaggcaaac accagcgaaa tggacggcgt tacgtaggag
     7261 aggatcacca gcgaacgcgc ggttttgcgc aggcggaact cgcggttgaa aaacatcgcc
     7321 acggccagcc cgagcacggt gctgcccgcc accaccagcg ccgtatacca gaccgtcatc
     7381 cacagcgagt gccagaagcc gggatcggag agaatgcgca catagttact caccccaaca
     7441 aacgttgact cgatgttggg attgagcggc agacgtaaaa aactgatttc gatattcgac
     7501 accatcggcc acgccaccag accgcccagc agtaataagc tgggcgcgag aagcagcagc
     7561 gcaaacggca tatcggaacg acctgaaaaa agcctgttca ttgcggattc ctgtcctggg
     7621 tttagtgctg ttcgatcagt tcatccagct ttttctggct cgcttgcagc gtgccaggga
     7681 gatcggcttt acccacggtg acgttatgca ccatggagct caccacacca gaacccgtca
     7741 catcgcccat gcgggtaaag tttttatccc ctactgcgcc aaagacctga atatttggca
     7801 gttcaccgat tagctgattc ggtagttcac ccagcgcctt aataacgtcg ttgtctttcc
     7861 aagtggcggt agtgacaacc gctttattca ccggcagcgc agcacctggc gacatcatca
     7921 cccagtcggc aatgttgtcc gcctgctcca taaaggtgac aaatttttct gctgcttcgg
     7981 tcttttgtcc ggcggtaatg gtcagcgagg tcaacatgcc gtagaccgca gagtttttct
     8041 cggttggcac cacgaaaccg acgtttttcg ggtcgccttc tttaatcaca gccggaagga
     8101 tataggtgga gtaaatcgcc atcggcgcgg tgccgttcat aaaggcgtct ttcacttcca
     8161 tgatgtcgtt agaacccggc atggtgttgg cagcaaggtc gcggtaatag gtcagcgcct
     8221 gcatcatctc tggtgtatca agggtgattt tgccttcggc gttaaagacg ttggcctagt
     8281 tggataacgc aaactgggag aaggattgtt ccgtcagcac gctttctgcc gtaggcagcg
     8341 caataccgta ttttttattc gccgggtcat tcagtttctg tgcaacgtcc agcagctgtt
     8401 gccagttttt cggctcctcc agccccgctt ttgccagcac atctttgcga taccagatgc
     8461 cgccaatcca ggcgctgaca ggaacaccgg tccatgcgct accatcttcg gtacgcacaa
     8521 tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt gctgatgacc gtggcaaccg
     8581 ctttgcgatc gataagctgc tctttatcca taactttggc gtagtcatgg ctggtttcga
     8641 tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac tttggtgtta taagcgtctt
     8701 cttccacggg cacttgttta acggtgatac ctgggttttc tttttcaaaa cgggcaatca
     8761 atttgctgat aactgcctga cgctcctgct ccaccgaaga atgcataaat tcgatggata
     8821 catgggtttt attttcttct ttacagcctg aaatcagggc gcaggaaacc agtgctgata
     8881 acagcacgat ttttgattta atcatgagat gttccttttt atttaatcca cataacctgc
     8941 caagggcgca gcgttatttc attacccaat atatcaactt cactaatcag atcccgacca
     9001 tgcagatcag taatatttac atgctgaata tttttactga cattaaacaa tccagtcaga
     9061 caattaccat cagcgttact tctttgaata cgcattacgg atgaattaac cgtatcgatg
     9121 gtaaaattat tatccggatg aaactcgtta tggctgcgac gaagtgtaat taaacgcgac
     9181 aactcatgat ataccgcatg ccttaatgta gcttcatcgt tcagttctcg tgttatctct
     9241 tcactgtgat attttttacg gttaatcgca cggttatatc cgagtttttc gacacctgcg
     9301 taatcattac gcgagccaag aatactttga atatatatcg cgggtacacc ggggaaactt
     9361 aacaaaatcg catgggcaag gataaacctg gcgcaacgtt cttcatcgct actctcgcgg
     9421 cggcttaacg catccatata ggtgacattc atctcatagg gactgcgcgt gccgtcggga
     9481 ttatttttcc agtttacta
//
LOCUS       DAJVQH010000136         9495 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJVQH010000136 DAJVQH010000000
VERSION     DAJVQH010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.136"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..436
                     /locus_tag="OQM87_003504"
     CDS             <1..436
                     /locus_tag="OQM87_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1509499.1"
                     /translation="TKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRG
                     QQPGGAAKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRA
                     MVSQDRQLVSLPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            454..1092
                     /locus_tag="OQM87_003505"
                     /pseudo
     CDS             454..1092
                     /locus_tag="OQM87_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1383..2111
                     /gene="mpaA"
                     /locus_tag="OQM87_003506"
     CDS             1383..2111
                     /gene="mpaA"
                     /locus_tag="OQM87_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCS1509500.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2086..3051)
                     /gene="ycjG"
                     /locus_tag="OQM87_003507"
     CDS             complement(2086..3051)
                     /gene="ycjG"
                     /locus_tag="OQM87_003507"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCS1509501.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3170..3676
                     /gene="tpx"
                     /locus_tag="OQM87_003508"
     CDS             3170..3676
                     /gene="tpx"
                     /locus_tag="OQM87_003508"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCS1509502.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3720..5261)
                     /gene="tyrR"
                     /locus_tag="OQM87_003509"
     CDS             complement(3720..5261)
                     /gene="tyrR"
                     /locus_tag="OQM87_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCS1509503.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5409..6470)
                     /locus_tag="OQM87_003510"
     CDS             complement(5409..6470)
                     /locus_tag="OQM87_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCS1509504.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6467..7864)
                     /locus_tag="OQM87_003511"
     CDS             complement(6467..7864)
                     /locus_tag="OQM87_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCS1509505.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8020..9018
                     /locus_tag="OQM87_003512"
     CDS             8020..9018
                     /locus_tag="OQM87_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1509506.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9129..>9495)
                     /gene="ompG"
                     /locus_tag="OQM87_003513"
     CDS             complement(9129..>9495)
                     /gene="ompG"
                     /locus_tag="OQM87_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCS1509507.1"
                     /translation="TFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYLPLTLGN
                     HSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYAFEWQDH
                     DEGDSDKFHYAGVGVNYSF"
BASE COUNT         2400 a         2539 c         2277 g         2279 t
ORIGIN      
        1 aacaaaagag gctcgtggtg gagaattaca gatcaacgaa ttacttcccg ccagcctgga
       61 tgcagcaaaa gcccatggac tgaccgaacg cgatgtttat gaagcaactg actactataa
      121 aacaccacgc gggcaacaac cgggaggtgc ggcaaaaatg ttgttctcac atgcacagaa
      181 aacgctggcc tgggatgcct tcgcatttac tgaagtgtta ctgacccagc cggtgatggt
      241 agtagttggt gaaaaagtcg gtgccttcgg tgcttatcgt gatggactgg aagtctatgg
      301 ccgggcgatg gtttcgcagg atcgtcagtt agtttcgtta cccgatttct cacattatga
      361 actatatgac aaaccggaag cagttcagga ggcgctggca aaagttattc cttttttcaa
      421 cacccatctg ggataagttt catgagaaat attatgaaaa atgtactgat tcttggtgct
      481 ggcggtcaga tagcccgcca tgtgattaat caactggcag ataaacaaac tattaaacaa
      541 actttatttg cccgtcagcc agcaaaaata cacaaaccct atccaactaa tagccagatt
      601 attatggggg atgtactgaa tcatgcagca ctgaagcagg ccatgcaggg acaggatatt
      661 gtgtatgcca accttacggg ggaagattta gatattcagg caaattcagt gatcgctgcg
      721 atgaaagcct gtgatgttaa acgtctgatc tttgttcttt ccctcggtat ttatgatgaa
      781 gttccaggaa aatttgtcga atggaataat gcagttattg gtgaacccct aaaaccattc
      841 cgcagggctg cggatgcaat tgaggcttct ggactggaat atacaattct tcgccctgcc
      901 tagctgactg atgaagatat tattgattat gagcttacct ctcgcaatga accattcaaa
      961 ggtacgatcg tatcacgtaa gagtgtcgca gcattaatta cagatatcat cgataaacct
     1021 gagaaacaca ttggtgagaa tataggcatc aatcaacccg ggactgatgg cgataagcca
     1081 ttcttcatgt aaatcaatta tatagatacc atcgtactgt actatgtgca gtacgattta
     1141 ttaaacttat taaggtgaac tgatcaatca gatgcgatta acatgtgtag aaccagtaag
     1201 aataaaatac gccatgtata taacattacg acaattctaa tatctgcaca tcaatgaaaa
     1261 tgcaaacaac ttccggcaaa aaagtgatac aagatcgatg acatagcgtt gaagaaaagg
     1321 atttgataat ccgcaaaatt tgcacaacac tgcctgtaat acttcctgaa ccagagacga
     1381 tcatgaccgt aacccgccca cgcgccgaac gcggcgcatt tccgcccgga acagaacatt
     1441 acggacgttc attattgggc gcgccgttaa tctggtttcc tgcccctgca gccagccgcg
     1501 aaagtggttt gattctggct ggcactcacg gtgatgaaaa ctcttcggtc gtcacgctct
     1561 cctgtgctct gcggactttg acaccttctt tgcgccgcca tcatgtggtg ctgtgtgtga
     1621 atcctgacgg ctgccagctg gggttacggg ccaatgctaa tggtgtggat ttaaacagaa
     1681 actttccggc ggcgaactgg aaggaaggtg aaacggttta tcgctggaac agcgccgctg
     1741 aagaacgtga tgtcgtatta ctgacaggtg acaaacctgg ctccgaacct gaaacccagg
     1801 cgctgtgcca gcttatacac cgcattcaac ctgcctgggt ggtctcattc cacgatccac
     1861 tggcttgtat tgaagatccc agacacagcg aattaggcga atggctggcc cagtcgtttg
     1921 aattgcctct ggtaaccagc gtcggttatg aaacgcctgg ctctttcggc agttggtgtg
     1981 cagatctcaa cttgcattgc atcaccgcag agtttccgcc catctcctcc gacgaagcca
     2041 gcgaaaaata tctctttgcg atggcaaact tgctgcgctg gcatcctaaa gatgcaattc
     2101 gcccgtcgtg aactgaagcg ccggttccac atctaccgcc agccaggtcg gtccgtcaag
     2161 atcggcgaaa ctgacctgcg gcaccagcgg taaagcggcg ctaatggcac gagaggtaca
     2221 caacatgcag cccagcatca gactgaaacc ttgtgcacgc gcttcagtcg ccagcgccag
     2281 cgcttccgtc agaccaccgg ttttatcgag cttaatgtta accatctcat agcgcccttt
     2341 cagcgccttc agattgctac gagtatgaca actttcatca gcacaaatcg gcaacggatg
     2401 aataaaattc tccagcgccg catcgtcctg cgcaggaagc ggttgttcaa gcatcgcaac
     2461 gcctaaatcc gccaatagct ggcaacgcgc cgccaaccct tctgcacgcc aggattcatt
     2521 tgcatcaacg atcagcgtgg catcgggcac agcggtgcga attgccacca tccgctcact
     2581 gataagatgg ttatccagct tcactttcag taatttcgcg cctgcctgcc agagtgttga
     2641 tgcactattg gccatctgat caggcgtacc gatgacaacc gtctgtgcag tgataactgt
     2701 ctcgggaagc gttatgccga tcaaatcagc cagcgattgc tgctgttttc gcgccgcgag
     2761 atcccacaat gcacaatcca gcgcattacg tgctgcgccg gcagggagaa ttttttgcaa
     2821 ctcctcccgt gtcagccctt tctctaattg cggcacgacg ctcataattt gcgccattac
     2881 cgaggcatca ctttctccat aacgcggata cggcgtgcat tcgccggtgc ctttaatacc
     2941 ctcttcttcc agttcaacca ccaccacgcg cgcttcactg cggcttcccc gggcaatcac
     3001 aaacggggta tgtaagggcc aggcttcctc aaatacctta acgattctca tctttactcc
     3061 ttgttgtcgc ggtggtctgc ggattaagcg caaatagcgt ttgctgtgtt attgacagtt
     3121 agcataaact aggtgtgacg ttaactatat gtaaacagga aagataatca tgtcacaaac
     3181 cgttcatttc cagggcaacc cggttacagt cgccaattcc atcccgcagg cgggtagcaa
     3241 agcgcagact tttactctcg tggcaaaaga tctgtctgac gtcaccctcg gtcagtttgc
     3301 gggtaaacgc aaagtgctga acattttccc gagtattgat accggtgttt gcgccgcatc
     3361 agtacgtaag tttaaccaac tggcaactga gatcgacaac accgttgtgc tgtgtatctc
     3421 tgccgatctg ccgttcgccc agtctcgttt ctgcggcgca gaaggtctga acaacgttat
     3481 caccctctcc actttccgta acgctgaatt tctgcaagcc tacggtgtgg caattgctga
     3541 tggcctactg aaaggtctgg cagcgcgtgc cgttgtggtt attgacgaaa atgacaatgt
     3601 gattttcagc cagctggtgg atgaaatcac caccgagccg gattacgaag cagctctggc
     3661 tgtactgaaa gcataagtta atatgcctga tggtgttgca ccatcaggca tattcgcgct
     3721 tactcttcgt tcttcttctg actcagacca tattcccgca acttattggc aatcgcggta
     3781 tgtgaaacgc cgagacgttt tgccagtttg cgcgtgctgg gataattgcg ataaagctgg
     3841 gttaataccg agcgttcaaa acggctggtg atttcgtcca gcgaaccttc catcgcatct
     3901 tcgcccacgg ctaccgttgc tgcgtcataa tccggcaaca aaatatcctg tggacgcagc
     3961 tcataaccgt ccagttgtgt cagcgcgcga tagatagcgt tctttaactg ccgcacattt
     4021 cccggccacg cataacgtgt aagtacagta ttcaggtcag cggccagttt cggacgcggc
     4081 acgccctgct cgtcggcaaa gcgggcgacg aacagctcag ttaacggcat aatgtcctgc
     4141 ggacagtcac gtagcggcgg cagattgagc gtcaacacgt tcagacgata atagagatct
     4201 tcacggaaca cgcctttttg caccagttcg accagattct tctgcgtagc gcaaatcacc
     4261 cgcacatcga catgcacctc atggtcttcg ccaacccgac ggaaagtgcc atcattaaga
     4321 aaacgcagta atttcgcctg catccgtggt gacatttccc ctatttcatc caacagcacc
     4381 gaaccaccgt tcgcctgctc aaagaaccct ttcttccctt ccggagcatg accaaacagt
     4441 tcactctcga ccgcatcttc tggtatagac gcgcaattca gcgccaggta aggtttgccc
     4501 gctctggggc ttgcctgatg gcaggcgtag gcaaaaagat ctttacctgt acctgtgtca
     4561 cccgtaatca gcagcggcgc gcttagcatc gccagtttct gcgcctgttc gacaacgtgc
     4621 ttcattttcg ggctgacggc gacaatttgg ctgaaggcgc tgacgtcctg ggcggcgaca
     4681 ttttgcaact ggcggcccat acgaatcgtt gatcgcaaca tcaccaccgc accggtcagg
     4741 acgtgttgat cattttcatc ctgaagataa acaggcgtaa tctccatcag gaaattctgc
     4801 ccattaataa cgacatgctc gttatgcgaa tcttgcggtt cgctttccag ccaacgtaaa
     4861 aaattaaagc cgttaatcaa ttgtgcggcg gtatggttgc gcaggcgatc caatttttgc
     4921 ccaaaaagct gacagctcgc cgggttcgcc atatccactt tgcttttcat atcgacagag
     4981 agcacaggtt caggcaacgc ctccagtaac gcgctcaacg ccagatgctc acgttcggaa
     5041 ggcatccacg ggacagtacg cacatcggta acacctgcaa tacggcgtat ttcggccatc
     5101 aaactgctga aactctcaaa ctccagttca gcaaaattga ggtagattcg cccaatggga
     5161 tcaatctcaa taccgcgtaa atcaatgcct cttagcacga gtagatcgag taattcgcgg
     5221 gtcagaccga gtcggtcttc acaaaagact tccagacgca taggaacctt cacctgaaaa
     5281 aagaacaatt aatatgatga tatgacacta tttggatagc aggaaggttt ctgtcaacaa
     5341 tcattgacac aaagacggaa aaacgggcac ttcggcgtaa agatatccgg tcagcacccg
     5401 atattgcatt atttttcgct gggcgttttg cctttttgca gagtttcttt cacctgaccg
     5461 ataagctgac gacggaaatc cccaaggcga ggtttgtcat cgtcaatcca cggcagcggg
     5521 cggcaaagct ccatagcttt aatcccgagt cgtgccgtca gaagtccagc tccaattccc
     5581 tgagctgcgc gggtagacaa acgagcagcg agatcttgcg acatccagtc catccccact
     5641 tcgcgcacca gttcgctggc tccggcaaaa gcgatattca gcaataccag cttaaacaga
     5701 cgcaaacggc tgtaataccc cagttcaatg ccatacagcg tggcaatgcg attaattaaa
     5761 cgcagattgc gccaggcgat aaacgccata tcgaccaacg ccagcgggct gaccgcaatc
     5821 atcaacgttg attccgccgc cgaacggctg atttcgcgcc gcgcctgggc atctaaaact
     5881 ggctggacca aatgcgcata caggctgacc acttcacgat cgttctgcgt ttcatggatt
     5941 gaggcatacc agcgttgcag cgccggatgc gactgatcaa tacccgcctg ctgcgccagt
     6001 ttttcgcaaa atgcgcgacc tttgcccgtg ccgtggctat gcaataaatc acgcgcttcg
     6061 tcgcgttcat gggcgcgctg tcgcaagcgc cataagcgcc gccactctgt taccacagaa
     6121 cctacgccag cgccgataat caatgcccca gcggcacatc cacccagcgc tacccagtcc
     6181 tgggtttgcc aggcattcat tgtccactgt ataccctgcc cgacaacgct tgcgccaaac
     6241 agagccagcc cgcccatcac cattttgcgc cacaggctgc gtttcggacg taacgctgcg
     6301 tccattaccg cttcgacttg cccctcttct tcctgcgctt cgtcgagcgt ggccggggca
     6361 aaattttgcg cctgattttc gtcaaaggtc tgctgcgccc tgaattttgg attctgttcg
     6421 acctccagcg gaccgtcgaa atcaatacgt ggttttaacg gttcggtcat cgcaatttat
     6481 ctcctattaa aaattccagc gcggcatcaa gacgaatatg cggcagcggt ttgtcgacat
     6541 ccatcacctg cgggcgaaac gcttcaaact ggaacccttg cttgtcccag aacgcctgac
     6601 cgggcaaacg cgcaggaact tcaccggggt aaacggtgag aggcgcacca tcgctgagcc
     6661 ggttaccgcg caatgccgga attttctcgc cgttgacgtc aataatgccg ctggtggtcg
     6721 tctgcaccga ggccagcccc aggcaatcca tactaatcct ttcaaacgcc gcattttgcc
     6781 aggcatcctg aatcagttgt tgcagtaatg aaaccatatt ggcgtgctga tcgaccgtta
     6841 catggtccgc tttcgtcgcg gcaaacagca gtttgtcgat aaccggcgag aataagcggc
     6901 gaaacagcgt gcgctgcccg tagtggaaac tttgcatcag ctgcgtcagt gccagacgca
     6961 tatcattaaa tgcctgtggc ccactgttga gaggttgcag gcaatccacc agcacaatct
     7021 ggcggtcaaa gcgcagaaaa tgattcttat agaacccctt caccactttc tcgcagtaat
     7081 aattaaaccg ctcgcgcagc attccggcat tggtgtgctt atcggcctgc gccagtttgg
     7141 actcgcccca ggcatcgaca tccggccacg ggaagaattg cagcgcgggc gcacctgcca
     7201 tatctcccgg caagacaaag cgcccaggct gaataaagtg cagcccctgc tgtttacagt
     7261 ggtggagata atcggtccac acggcggcaa tgtctgccag ccggttttcg tcggcaggcg
     7321 ctagcgggtc aagtccttcg cacatcattc gccatttcgc cgaccattcg ccgcgctgac
     7381 cattgagtaa gcccgtcatc tggcgcgacc agcttaagta atcctgcgcc agcatcggca
     7441 ggtcgagcaa ccattcgccg ggataatcca caatttccag atacagcgtg gaggtatcct
     7501 taaagtggcg cagcagcgaa tcgttcgatt taaaacgtaa cgccaggcga atttcactga
     7561 cgccgcgcgt aggcgtcggc caggcgggag gatcaccata taactgcgcc agtccttcat
     7621 cataggtgaa gcgcggaatg ccaaagtcac gctggggaat gcgttttacg cccagcaagc
     7681 gctcttcacg caccgcgctt aacagcggca aacgtgctcc ggcatgaata ttgagcaact
     7741 gattaaccat cgcggtgata aacgctgttt tgccgctgcg gctaagtccg gttacagcga
     7801 ggcgcagatg tctgtcgaca ccccgattca ccagcgcatt gagttcattt ttaagtcgct
     7861 tcattgctgt cctgtgctgc tctgaagacc agaaaaaaaa tcattcgtaa ggatatgaat
     7921 gtgggggcca ggccattgat tatcaactgc ccgtgttgtc atcgacgggc gaactccata
     7981 agatgtgcag cattcacgtt cggacggagt tcctgaccta tgtcccctac tatttatgat
     8041 attgccaggg ttgcaggcgt atcaaaatcc accgtatcac gcgtgctgaa taagcaaact
     8101 aatatctccc cggaagcgcg cgaaaaagtg ttacgggcca ttgaagaatt acagtatcag
     8161 ccaaacaagc tggcccgcgc gctgacctct tcgggttttg atgccattat ggtgatttct
     8221 acccgttcga ccaaaactac ggcgggtaat ccgtttttct ctgaagtttt gcatgccatc
     8281 accgccaaag cggaagaaga aggtttcgac gtgatattac aaacgtcgca caatccggca
     8341 gaagatctgc aaaaatgcga aagcaaaatt aaacagaaaa tgattaaagg cattattatg
     8401 ctcagttcgc cagcggatga gtcatttttt gcccaactcg ataaatacga tattccggtg
     8461 gtggtaattg gcaaagttga aggtcaatat gcccatgttt attctgtcga taccgataat
     8521 tttggtgaca gcattgcgtt gaccgatgca ttaattgaaa gtgggcatca aaatattgcc
     8581 tgcctgcatg ctccgcttga tgtccatgtt tcggttgatc gcgttaatgg ctataagaaa
     8641 agtctgtccg cgcataatat tgcagtgcgt gataaatgga ttgttgacgg cggttatacc
     8701 catgaaacag cactgcaagc agcacggcaa ttattaagtc agtcaccgtt gcctgaggca
     8761 gtgtttgcca ctgacagcct gaaattaatg agcatttatc gtgcggcggc agagaaaaat
     8821 attgctattc cgcagcagtt agcggtggtg ggttatagta atgaaacgct gtcgtttatt
     8881 ttaacgcctg caccaggcgg catcgatgtt ccgacgcagg agttaggtca acaaagctgc
     8941 gagttattat tccgcttaat ttcgggaaaa ccgtcaccac agaatattac cgttgccacg
     9001 catatgacgc tgaaataaat accagctacc ttgcctgaat gccggatgcg atgctggcgc
     9061 atcttatccg gcctacattt atagatcgga taaagtactc gcgccgcatc cggcaattta
     9121 gcccattatc agaacgagta atttacgccg acacctgcat aatggaattt atcgctgtcg
     9181 ccttcgtcgt gatcctgcca ctcaaacgcg tattccagcg aaacggaaag tccgttctgg
     9241 aaatcataac cgtaaaataa acccacacgg ttaaaatcat ggccttcacg ttcaatatca
     9301 tcctgccagt cccagttact ccagcgatcc agcccaatgc gcgtatacgg cgtaaccgag
     9361 tggttgccga gcgtcagcgg caaataggcg cgaatttctt gtgtggaaaa ctcaccgtta
     9421 ttgcggctgt cgtccatatt gaagccgcgc tcgagataat agttcactcg caaagcaacc
     9481 gtttcgttga aggta
//
LOCUS       DAJVQH010000137         9425 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJVQH010000137 DAJVQH010000000
VERSION     DAJVQH010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.137"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(208..552)
                     /locus_tag="OQM87_003514"
     CDS             complement(208..552)
                     /locus_tag="OQM87_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1509508.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            684..2594
                     /locus_tag="OQM87_003515"
     CDS             684..2594
                     /locus_tag="OQM87_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCS1509509.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQLGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2652..3347
                     /gene="tsaB"
                     /locus_tag="OQM87_003516"
     CDS             2652..3347
                     /gene="tsaB"
                     /locus_tag="OQM87_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCS1509510.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3387..3968
                     /gene="yeaY"
                     /locus_tag="OQM87_003517"
     CDS             3387..3968
                     /gene="yeaY"
                     /locus_tag="OQM87_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCS1509511.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRRQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4173..5858
                     /gene="fadD"
                     /locus_tag="OQM87_003518"
     CDS             4173..5858
                     /gene="fadD"
                     /locus_tag="OQM87_003518"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCS1509512.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            5940..7055
                     /gene="rnd"
                     /locus_tag="OQM87_003519"
     CDS             5940..7055
                     /gene="rnd"
                     /locus_tag="OQM87_003519"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCS1509513.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNARQLRTRQLACLQLLAD
                     WRLRKARECDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7109..8074)
                     /gene="yeaX"
                     /locus_tag="OQM87_003520"
     CDS             complement(7109..8074)
                     /gene="yeaX"
                     /locus_tag="OQM87_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCS1509514.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8130..8747)
                     /gene="yeaW"
                     /locus_tag="OQM87_003521"
                     /pseudo
     CDS             complement(8130..8747)
                     /gene="yeaW"
                     /locus_tag="OQM87_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(9089..>9425)
                     /locus_tag="OQM87_003522"
     CDS             complement(9089..>9425)
                     /locus_tag="OQM87_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509515.1"
                     /translation="NQENKNSQPDRSTLKTKLIAIVELLEDKELHSAIELFRKKGFEA
                     LMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQ
                     EPQDVKKAV"
BASE COUNT         2291 a         2308 c         2496 g         2330 t
ORIGIN      
        1 aaccagagtt gctaatcaaa gggcgaacac aacggcatgg ttatgtttat ttatatagtt
       61 agttttatgg ataaaattga aattataaat ataaataatg aattagcaga agcatcaaca
      121 gtgatatctt attctctggt cgatttatgt aaaaccgacc agtattatcg ataaattatc
      181 gtctgactta ttttgcttcg ataaagctta caccgcagcc acaatcttaa tttcaacttt
      241 atacttcggg ttcatcaaac ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac
      301 aacccaggca tcccacgctt tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat
      361 ggtggcatcc agaatgctcg atttattgct gccctgtttt tccagcacgg cgtcaatctg
      421 tgccagcgtg ttggcggttt gctcaaaggc atcggcgtcg aggttttccg gtacaccagt
      481 gtagtagagc gtgttgttgt ggattactac atcagaccag cgggcttcag catcgatacg
      541 aacgatagtc atcattttgt cctcattata cttccatttt cgtgctgcaa agactgccac
      601 aatgtgaggt tgccgtcacc acttcaactg gcgaaagcgc cctcatcctg acataatccc
      661 tgttcaaatc aacagggggt agtgtgacgg acgattttgc accagacggt cagctggcga
      721 aagcgatacc aggctttaag ccgcgagaac cacagcgaca gatggcggta gccgtcaccc
      781 aggcgataga aaaaggccag ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct
      841 acgcttacct ggctcctgcg ctgcgggcga aaaagaaagt cattatctcg accggctcaa
      901 aagcgttgca ggatcagctc tacagccgcg atttgccaac agtctcaaag gcattgaaat
      961 atacgggcaa cgtggcgctg ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg
     1021 aacagcaggc gctggcgggg ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc
     1081 tgcgctcctg gtctaatcaa acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg
     1141 aagattcaca ggcgtggccg ctggtcacca gcaccaacga caactgtctt ggcagcgact
     1201 gcccgatgta taaagattgc tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg
     1261 tggtggtggt aaaccatcat ctctttctgg cggatatggt ggttaaagag agtggatttg
     1321 gcgagttgat ccctgaagcg gacgtcatga tcttcgacga agcccaccag ctaccggaca
     1381 ttgccagcca gtattttggt cagtcactct ccagtcgaca actgctcgac ctggcaaaag
     1441 acatcaccat cgcctaccgc accgaattaa aagacaccca gcagttacaa aagtgcgctg
     1501 atcgtcttgc ccagagtgcg caggattttc gtctgcaact cggtgagcca ggttatcgcg
     1561 gtaacctgcg tgagctgtta gctaatccgc aaattcagcg ggcattttta ctgctcgatg
     1621 acaccttgga actttgttat gacgtggcga aactgtcact ggggcgttcc gccttgctgg
     1681 atgcggcatt tgagcgcgcc acgttgtatc gcacacggct gaagcggcta aaagagatca
     1741 atcagccggg ctacagctac tggtacgaat gcacttcgcg ccattttact ctggctctca
     1801 cgccgctcag cgtggcggat aaattcaaag agttaatggc gcaaaagccc ggtagctgga
     1861 tcttcacctc agcaacgctg tcggtgaacg acgatctgca tcatttcacc tcgcggcttg
     1921 ggatagaaca ggcggagtcg ttgctattgc caagcccgtt tgattacagc cgccaggcgt
     1981 tactctgtgt gccgcgcaat ctgccgcaaa ccaaccagct aggttctgct cgccagttag
     2041 cggcaatgct gcgaccgatc atcgaagcta acaacggtcg ttgttttatg ctttgtacct
     2101 cgcacgccat gatgcgcgat ctggccgagc agttccgcgc taccatgacg cttcccgttt
     2161 tgttgcaggg ggaaaccagt aaaggccaac tgttgcagca gtttgtcagt gccggtaatg
     2221 cgcttcttgt ggcaaccagt agtttctggg aaggggtgga cgtgcgtggc gatacattgt
     2281 cattggtaat tatcgacaaa ttgccgttta cctcgctgga tgatccactg ttaaaagcgc
     2341 gcatggaaga ttgtcgtttg cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg
     2401 ccgtcattac tctcaaacag ggggtaggtc gactgattcg cgacgccgac gatcgtggcg
     2461 tgctggtgat ttgtgactat cggctggtga tgcgtcctta cggcgcgacg tttctcgcca
     2521 gtctgccgcc cgcgccacgc acccgtgaca ttgcccgagc ggttcgtttc cttgcgatac
     2581 catcctccag gtaatttgtt acggagtatg gtatgatgcg cgccttttta ttgatctaag
     2641 cacgaagacc catgcgaatt ctggctatcg ataccgcgac agaggcctgc tctgtcgccc
     2701 tgtggaacga cggtactgtc aacgctcatt ttgagctttg ccctcgtgaa catactcaac
     2761 gaatcttacc gatggtgcag gatatcctga ccaccagcgg aacttccctg actgatatta
     2821 acgctctggc ttacgggcgc ggccccggta gctttaccgg cgtgcgcatt ggtattggca
     2881 tcgcgcaagg gctggcgctt ggcgcggaat taccgatgat tggcgtctcc acgctaatga
     2941 cgatggcgca aggggcgtgg cgcaaaaacg gcgcaacacg cgtgctgtca gccattgacg
     3001 cgcgaatggg cgaagtttac tgggccgaat atcagcgtga tgaaaacggt atctggcacg
     3061 gtgaagaaac cgaagccgta ctcaaacccg aactcgttca tgaacgaatg caacagcttt
     3121 ccggcgaatg ggtgacggta gggacgggct ggcaagcctg gccggatctc ggtaaagaga
     3181 gcgggctggt tttgcgcgat ggcgaagtgt tactccccgc tgcagaagat atgctgccga
     3241 ttgcttgtca gatgtttgcc gagggtaaaa cggtggcggt ggaacatgcc gagccggttt
     3301 atttgcgtaa caacgtcgca tggaagaaac ttccgggcaa agaatgaatc ttaagagtat
     3361 gccctgagaa aaaaaggagt cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac
     3421 tggcaggtac gtttgcgcta atgctgagtg gttgtgtcac tgtgccggac gccattaaag
     3481 gcagcagtcc cacgccgcaa caagatttag ttcgggtgat gagtgcgccg cagctgtacg
     3541 ttggtcagga ggcacgcttt ggtggcaaag tggttgcggt acaaaaccag caagggaaaa
     3601 cccgcctgga aattgctacc gtaccgctgg acagcggagc taggccgacg ctgggagaac
     3661 cttctcgcgg tcgcatttat gccgatgtga acggttttct ggacccggtg gatttccgta
     3721 gacaactggt tacggtagtc gggccaatca ccggtgcggt tgacggcaaa atcggcaata
     3781 cgccctataa atttatggtg atgcaagtaa cgggttacaa acgttggcat ttaacccagc
     3841 aggtgattat gccgcctcag ccgattgatc catggtttta tggcggtcgt ggctggccct
     3901 atggctacgg cggatggggc tggtataatc ccggccccgc gagagtacaa acagttgtaa
     3961 ccgaataatt gcttgttttt aaagaaaaag aaacagcggc tggtccgctg tttctgcatt
     4021 cttacggtaa agataaaaat aaatagtgac gcgcttcgca accttttcgt tgggtaatta
     4081 tcaagctggt atgatgagtt aatattatgt taacagcgtg tatatcattt ggggttgcga
     4141 tgacgacgaa cacgcatttt agaggtgaag aattgaagaa ggtttggctt aaccgttatc
     4201 ccgcggacgt tccgacggag atcaaccctg accgttatca atctctggta gatatgtttg
     4261 agcagtcggt cgcgcgctac gccgatcagc ctgcgtttgt gaatatgggg gaggtgatga
     4321 ccttccgcaa gctggaagaa cgcagtcgcg cgtttgccgc ttatttgcaa caagggttgg
     4381 ggctgaagaa aggcgatcgc gttgcgttga tgatgcctaa cttattgcaa tatccggtgg
     4441 cgctatttgg cattttgcgt gccgggataa tcgtcgtaaa cgttaacccg ttgtataccc
     4501 cgcgtgagct tgagcatcag cttaacgata gcggcgcatc ggcgattgtt atcgtgtcta
     4561 actttgccca cactctggaa aaagtggttg ataaaaccgc cgttcagcac gtaattctga
     4621 cccgtatggg cgatcagcta tctacggcaa aaggcacggt agtcaatttc gttgttaaat
     4681 acatcaagcg cttggtgccg aaataccatc tgccagatgc catttcattt cgtagcgcac
     4741 tgcataacgg ctaccggatg cagtacgtca aacccgaact ggtgccggaa gatttagcct
     4801 tcctgcaata caccggcggc accacaggtg tggcgaaagg cgcgatgctg actcaccgca
     4861 atatgctggc gaacctggaa caggtcaacg ccacctatgg gccgttgctg catccaggta
     4921 aagaactggt agtaacggca ctgccgctgt atcacatttt tgccctgacc attaactgcc
     4981 tgctgtttat cgaactgggt gggcagaacc tgcttatcac taacccgcgt gatattccag
     5041 ggttggtaaa agagttagcg aaatatccgt ttaccgctat cactggcgtt aacaccttgt
     5101 tcaatgcgtt gctgaacaat aaagagttcc agcagctgga tttctccagt ctgcatcttt
     5161 ccgcaggcgg tgggatgcct gttcagcaag tggtggcaga acgctgggtg aaactgaccg
     5221 gacagtatct gctggaaggt tatggcctga ccgagtgcgc gccgctggtg agcgttaacc
     5281 cgtatgatat tgattatcat agcggtagca tcggtttgcc ggtgccatcg acggaagcca
     5341 aactggtgga tgacgacgac aacgaagtac caccgggtca accgggtgag ctttgtgtca
     5401 aaggaccgca ggtgatgctg ggttactggc agcgtccgga tgctacagat gagatcatca
     5461 aaaatggctg gttacacacc ggcgacatcg cggtgatgga tgaagaagga ttcctgcgca
     5521 ttgtcgatcg taaaaaagac atgattctgg tttccggttt taacgtctat cccaacgaga
     5581 ttgaagatgt cgtcatgcag catcctggcg tacaggaagt cgcggctgtt ggcgtacctt
     5641 ccggctccag tggtgaagcg gtgaaaatct tcgtagtgaa aaaagatcca tcgcttaccg
     5701 aagagtcact ggtgactttt tgccgccgtc agctcacggg atacaaagta ccgaagctgg
     5761 tggagtttcg tgatgagtta ccgaaatcta acgtcggaaa aattttgcga cgagaattac
     5821 gtgacgaagc gcgcggcaaa gtggacaata aagcctgagc gttaagtcag tcgtcagacg
     5881 ccggttaatc cggcgttttt tttgacgccc actaaagaga aaacaatttg aattaccaaa
     5941 tgattactac ggacgatgcg ctggcttctt tgtgtgaagc cgtccgtgcc tttccggcga
     6001 tagccctgga tactgaattt gttcgtacgc gcacttatta cccgcagctg gggttgattc
     6061 aacttttcga tggcgagcat ctggcgctaa ttgatcctct cgggatcacc gactggtcac
     6121 cgctgaaagc gatcctgcgc gatccgtcca tcacaaaatt tctccatgca ggcagtgaag
     6181 atctggaagt gttcctcaat gtctttggcg aattaccaca acccttgatt gacacgcaaa
     6241 tccttgctgc cttctgcgga cgcccgatgt catggggttt cgcttccatg gtggaagagt
     6301 attccggcgt tacgctggac aagagtgaat cgcgcgccga ctggctggcc agaccgctga
     6361 ccgaacgtca gtgtgaatac gcagcggcgg atgtctggta tctgttaccg atcactgcca
     6421 agcttatggt agaaacggag gcctccggct ggctacctgc ggcgctggat gaatgccgcc
     6481 tgatgcaaat gcgtcgtcag gaagtcgttg cgccggaaga tgcctggcgt gatatcacca
     6541 atgccaggca gttacgcaca cgccaactgg cctgtctgca actgttagcc gactggcgac
     6601 tgcgcaaggc gcgagagtgt gatctggcgg tgaactttgt cgtgcgtgaa gagcatttgt
     6661 ggtcggtcgc gcgttatatg ccgggaagtt taggcgaact ggacagcctg ggtttatccg
     6721 gtagcgaaat ccgctttcac ggtaaaacgc tgctggcgct ggtggaaaaa gcgcagacat
     6781 tgccggaaga tgccttaccg cagccgatgt ttaacctgat ggacatgcct ggttatcgta
     6841 aagcgtttaa agcgattaag tcgctgatta ctgacgtgag cgaaacgcat aagatcagcg
     6901 ccgaattgct ggcatcgcgt cggcaaatca accaactgct gaactggcac cggaaactga
     6961 aaccgcagaa caatttgccg gagctgattt ccggctggcg tggtgagctg atggcggaag
     7021 cattacacaa tttattgcag gaatatccgc agtaaaatct tccgaagccg gactgggcgc
     7081 gctcagcgcc acatccggct ttggcaaact acaaatccaa caccaggcgt ttacccttcg
     7141 cacgcgaaca acagatcaac atactttgct ggctggcacg ctcttcatcg ctaaaatact
     7201 gatcccgatg gtcagcttca ccttccagta ttgccgtttc gcaggttccg catacccctt
     7261 cacgacataa acattccact ttcgcggctt tattattttc aataacctgc aaaatagtca
     7321 tctcttccgg caccacaaac tcttttccgg aacgggcaag caccagggta aatgcatcgc
     7381 cggttttgtc ttcgatagca aattgctcaa agtgcagcgt atcggcggcg atgtccagac
     7441 gcgcagcttc acttcttacc gcttcaatta gcgcctcggg gccacaggta taaacgtgtg
     7501 tgccaggttc gatatccgcc aataatcgcg ctaattccag tcgtgttccg gttgatgaat
     7561 gatgcaaatg gactttctca gcctgcggat gctggactag ctcatcacga aatgcgcaac
     7621 tttctggatt tcgcgagcag taatgtagct gccagtcgat gtcgctgtgt tgcaactctg
     7681 ccatgtgcga caggaaaggg gtgataccaa taccgcccgc gataaacaga tgctttctgg
     7741 ctgagggaat tagcgcaaac aggttattag gcgttgagat cgttaaccga tcgcccactt
     7801 ttacctgctg atgcaaaaag cgggaaccgc cgcgcgagtt ttcttccagt cgaactgcaa
     7861 tctgataact agaggtgtta tgcggtgaac tcagtagtga atacgcattg ctgtactggt
     7921 tatcaccatc gctcatctgc actaggatgt gacttcctcc ggtgaacgca ggcagcggtt
     7981 tgccatcggt tgccaccagc gtgaagcgtt tcacctgttc ggtaaggggt tcaacctggc
     8041 tcacctgtac ttcaaacatt tgatagtctg acattgctgt actccatcaa aaatcagccc
     8101 agcggcggaa aataccgccg ccgatgtcat tagtccttaa acacctgcgc cagcagatta
     8161 tggaaatggg cgataccatg ttcggaaatg ccactgccgc tactgtcggc catgatgcgc
     8221 ccctgaccac gatagccacg cgatttcagc cctttctgta cgctttcaac cagacgtaaa
     8281 tcttccggat ggaacacatc gcgataccac tcaatcagcg atttttgctc gttggttaac
     8341 tcttcattgg tgaagtaaat atcgtagttt tgcagggtag tttcagaatc caccgggaat
     8401 tcataaatga ccgtcatcat ccctttgatc ggggtgacgt tcagcatcgt gcacggccac
     8461 agccagaaac cgtggaatgc cgcatccgta ccctcttcaa atttaaacga ctgttcggac
     8521 ggtttggcga aaccgtattg cagcgtccag ttaccgtgca tggtgtgcca gtaacgatca
     8581 acctgtacgg agtcggagaa acctagatgc gccggaccac agtgatagca ctcgagatag
     8641 ttatcgacaa tgttcttcca gttggcaggc gtgcgggtgg taaagcgggc cgctagtttc
     8701 agattgtgga cttccgggca ggcttccagc actttcgcgc ccagaccggt gtattgattc
     8761 acttgaagta cgaaaaaaaa ccgggaggac attggattat tcgggttctg atgggattag
     8821 atttggtggg gcttgcaagc ctgtagcgca aattttagtc gttaatcaat gaaacgcgaa
     8881 agatagtaaa aaattgcttt tgtttcattg aaaatacgaa aaacaaaaac actgcaaatc
     8941 atttcaataa cagcttcaaa aaacgttcaa aaccgataac aaccaggctg taaccaaatg
     9001 actcatatca caaatcagct catgccgttt aggtatgtta catgtgtgat tatgtgaggt
     9061 gaagtatgtt ttagctggtt catggttgtt atacggcttt ttttacatct tgtggttctt
     9121 gtgaaggagc tacaagacct tcctgttcat aaataccata ctttgacaaa atctttttac
     9181 gagtatctgt tggtaatgat tcaagcattt tggctgtttg taaggtttgc tgcgaaatac
     9241 cttgcaaatc agcctggatg gaatcatcgg aagaaaaaat ctcaggcata agagcttcaa
     9301 atcctttctt tctgaacaac tcaatcgcag aatgcagttc cttgtcctct aacaattcga
     9361 caattgcgat cagtttagtt ttcaatgtgc tgcggtcagg ttgagaattt ttattctctt
     9421 gatta
//
LOCUS       DAJVQH010000138         9342 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJVQH010000138 DAJVQH010000000
VERSION     DAJVQH010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.138"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            284..1402
                     /locus_tag="OQM87_003523"
                     /pseudo
     CDS             284..1402
                     /locus_tag="OQM87_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
     gene            1468..1716
                     /locus_tag="OQM87_003524"
     CDS             1468..1716
                     /locus_tag="OQM87_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCS1509516.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLSAIEYFVLRFIWRRWFSLAD"
     gene            1781..2149
                     /locus_tag="OQM87_003525"
     CDS             1781..2149
                     /locus_tag="OQM87_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1509517.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPRYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2243..2896
                     /gene="nfsB"
                     /locus_tag="OQM87_003526"
     CDS             2243..2896
                     /gene="nfsB"
                     /locus_tag="OQM87_003526"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCS1509518.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3004..4251
                     /gene="ybdG"
                     /locus_tag="OQM87_003527"
     CDS             3004..4251
                     /gene="ybdG"
                     /locus_tag="OQM87_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCS1509519.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4319..5695)
                     /gene="pheP"
                     /locus_tag="OQM87_003528"
     CDS             complement(4319..5695)
                     /gene="pheP"
                     /locus_tag="OQM87_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCS1509520.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5796..8931)
                     /gene="cusA"
                     /locus_tag="OQM87_003529"
                     /pseudo
     CDS             complement(5796..8931)
                     /gene="cusA"
                     /locus_tag="OQM87_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(8943..>9342)
                     /locus_tag="OQM87_003530"
     CDS             complement(8943..>9342)
                     /locus_tag="OQM87_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCS1509521.1"
                     /translation="PESIAWLVKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTL
                     QLRLEVDNADEALIDTGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKV
                     VSGGLFLIDSEANISGALERMRSESATHAH"
BASE COUNT         2286 a         2595 c         2273 g         2188 t
ORIGIN      
        1 acaaaatagc tgtttattta tacagcaaat cctgtttcgg acttctcact ccccatttga
       61 tcaactgaag ctgaacccgt ttttttctgc atgcctcaat cgtcaaaatt cttcgccacc
      121 agccagctct gccataactt gccacattga aaaagtagcc aaagtgcttc aggcaccatc
      181 agttcaccat caccaaaaaa aagaaacatt ccagaacttc cgcatctcgc ccagccgctc
      241 cgacttctat actgaataga aaacgtcaac ttaagagaaa cctatgccat tacccgattt
      301 tcatttttct gaacctttta ccctcggtat tgaactggaa atgcaggtgg ttaatccgcc
      361 gggctatgac ttaagccagg actcttcaat gctgattgac gcggttaaaa ataagatcac
      421 ggccggagag gtaaagcacg atatcaccga aagtatgctg gagctggcga cggatgtttg
      481 ccgtgatatc aaccaggctg ccgggcagtt ttcagcgatg cagaaagtcg tattgcaggc
      541 agccgcagac catcatctgg aaatttgcgg cggtggcacg cccccgtttc agaaatggca
      601 gcgtcaggag gtatgcgaca acgaacgcta tcaacgaacg ctggaaaact ttggctatct
      661 catccagcag gcgaccgttt ttggtcagca tgtccatgtt ggctgtgcca gtggcgatga
      721 cgccatttat ttgctgcacg gcttgtcacg gtttgtgccg cattttattg ccctttccgc
      781 cgcgtcgcca tatatgcagg gaacggatac gcgttttgcc ccctcacgac cgaatatttt
      841 ttccgccttt cctgataatg gcccgatgcc gtgggtcagt aactagcaac aatttgaagc
      901 cctgtttcgc tgtctgagtt acaccacgat gatcgacagc attaaagatc tgcactggga
      961 tattcgcccc agtcctcatt ttggcacggt ggaggttcgg gtgatggata ccccgttaac
     1021 ccttagccac gcggtgaata tggcgggatt aattcaggcc accgcccact ggttactgac
     1081 ggaacgcccg ttcaaacatc aggagaaaga ttacctgctg tataaattca accgtttcca
     1141 ggcctgtcgc tatggtctgg aaggcgtcat taccgatccg cacactggcg atcgtcgacc
     1201 gctaacggaa gataccttgc gattgctgga aaaaatcgcc ccttcagcac ataaaattgg
     1261 cgcatcgagc gcaattgaag cactgcatcg ccaggtcgtc agcggtctga atgaagcgca
     1321 gctgatgcgc gatttcgtcg ccgatggcgg ctcgctgatt gggctggtga aaaagcattg
     1381 tgagatctgg gccggtgact aagccgggaa ttgccttaca gaagaatatc cctgacaatg
     1441 gtgttttaac ttatatttaa caatgttatg aaacaccctt tagaaacctt gaccaccgca
     1501 gcaggcattt tgctgatggc tttcctctct tgcctgctgc tgcccgcccc cgcactgggg
     1561 ctgacgctgg cacaaaaact ggtgaccatg ttccatctga tggatcttag ccagctttac
     1621 actttattgt tttgtctgtg gtttttagtg ctgagcgcta ttgagtattt tgttctgcgc
     1681 tttatctggc gacgctggtt ctcgctggcg gattaaacgt cgattatcgc cgaacttcgc
     1741 ggcatacttt gcttattctc cttcgcctaa aggaacgttt atggataagc aatcactgca
     1801 cgaaacggcg aaacgcctag ctcttgagtt accctttgtc gagctttgct ggccttttgg
     1861 cccggagttc gatgttttta aaattggcgg caagattttt atgctgtcgt cggagctacg
     1921 cggcgtcccc tttatcaatc tgaagtccga tccacaaaaa tccctgttaa atcagcaaat
     1981 atacccaagc attaagccaa ggtatcacat gaataaaaag cactggattt cggtgtattc
     2041 cggcgaggaa atctccgaag cgttacttcg cgatctgatc aacgattcgt ggaatctggt
     2101 ggttgatggt ctggctaaac gcgatcaaaa aagagtgcgt ccaggctaaa gcgggaatct
     2161 atagcgcatt tttctcgctt accatttctc gttgaacctt gtaatctgct ggcacgcaaa
     2221 attacttaca catggagtct ttatggatat catttctgtc gccttaaagc gtcattccac
     2281 taaggcattt gatgccagca aaaaacttac cccggaacag gccgagcaga tcaaaacgct
     2341 actgcaatac agcccatcca gcaccaactc ccagccgtgg cattttattg ttgccagcac
     2401 ggaagaaggt aaagcgcgtg ttgccaaatc cgctgccggt aattatgtgt tcaacaaacg
     2461 taaaatactt gatgcctcgc acgtcgtggt gttctgtgca aaaaccgcga tggacgatgc
     2521 ctggctgaag ctggttgttg accaggaaga tgccgatggc cgctttgcca cgccggaagc
     2581 gaaagccgcg aacgataaag gtcgcaagtt cttcgccgat atgcaccgta aagatctgca
     2641 tgatgatgca gagtggatgg caaaacaggt ttatctcaac gtcggtaact tcctgctcgg
     2701 cgtggcggcg ctgggtctgg acgcggtgcc catcgaaggt tttgacgccg caatcctcga
     2761 tgcagaattt ggtctgaaag agaaaggcta caccagtctg gtggttgttc cggtaggtca
     2821 tcacagcgtt gaagatttta acgctacgct gccgaaatct cgtctgccgc aaaacaccac
     2881 cttaaccgaa gtgtaattct ctcttgccgg gcatctgccc ggctatttcc cctcagattc
     2941 tcctgatttg cataaccctg tttcagccgt catcataggc tgctgttgta taaaggagac
     3001 gttatgcagg atttaatatc ccaggttgaa gatttagcgg gtattgagat cgatcacacc
     3061 acctcgatgg tgatgatttt cggtattatt tttctgaccg ccgtcgtggt gcatattatt
     3121 ttgcattggg tggtactgcg gaccttcgaa aaacgtgcca tcgccagttc acggctttgg
     3181 ttgcaaatca ttacccagaa taaactcttc caccgtttag cttttaccct gcagggaatt
     3241 atcgtcaata ttcaggcggt attctggctg caaaaaggca ccgaagcggc agatattctg
     3301 actacctgcg cgcagttgtg gatcatgatg tatgcgctgc tttcagtctt ctcgttgctg
     3361 gatgttattt tgaatctggc gcagaaattc ccggcggcat ctcagttacc gctgaaaggg
     3421 atatttcagg ggattaaact gatcggcgca atcctggtcg gcattttgat gatctcgctg
     3481 ctgattggtc agtcgccagc gattctgatc agcggtcttg gtgcaatggc tgccgtgctg
     3541 atgttggtat ttaaagatcc gattcttggt ctggtggcag gtattcagct ttccgcgaac
     3601 gatatgctga aactgggcga ctggctggaa atgccgaaat acggcgcgga tggcgcggtg
     3661 atcgatattg ggttaaccac cgtcaaagtg cgtaactggg acaataccat taccactatt
     3721 cccacctggt ctctggtttc tgactccttt aaaaactgga gcgggatgtc agcatctggc
     3781 gggcgacgta ttaagcgcag tatcagtatt gatgtcacca gtattcgttt tcttgatgaa
     3841 gacgaaatgc aacgtctgaa taaagcgcat ttgttaaagc cttatttaac cagccgccat
     3901 caggaaatta atgagtggaa tcgccagcaa ggttctacgg agtcggtatt aaacctgcgc
     3961 cgaatgacca atattggaac ctttcgtgcc tatctgaacg aatatctgcg taaccatccg
     4021 cggattcgta aagatatgac cttaatggta cgccaactgg ctccaggtga taacggttta
     4081 ccgctcgaga tctacgcgtt tactaacacc gtggtgtggc tggaatatga aagcattcag
     4141 gctgatatat tcgatcacat atttgcgatt gtcgaagagt ttggtctgcg acttcatcag
     4201 tcgccaaccg gcaatgatat tcgctctctg gcgggtgcat ttaagcagta attaaaaaaa
     4261 ccgctctcat cgaatggatg agagcggaca tcgttttgtc ggatgtagcg tgaatgcctt
     4321 atttccgacg cagcgtttta aatgccacaa acaggaatac aatccacacc ggcagcagga
     4381 tcgctgacaa gcgcatatca tccatcgtgc acatcagcag caaaatcatg ccgaggaagg
     4441 cgatgcaaag atagttgccg aacggataga gcagcgcctt aaactgtgtt tcacgcccct
     4501 gacgtcgcat cgccgcgcga aaacgcagat gcgccaggca gatcataatc cagttcaaca
     4561 gcaacgttgc taccaccagc gtcatcagca gaccaaacgc tttttgcggc agcagatagt
     4621 tgattaacac caccagcgaa gtgatcgctc cggaaagcat cagcgagtta atcggcacac
     4681 cgcgacggct gacgcgagtc aaaaacttcg gcgcattacc ctgcacagaa aggccaaaca
     4741 gcatgcggct gttagagtaa accccgctgt tatacaccga cagcgatgcc accagaatga
     4801 cgaagttcag cgcagaagct accacattgc tgtcgagatt atggaaaatc atcacaaacg
     4861 ggctactgtt ggatttcact tccacccacg gatagagtgc cagtaaaacc accagtgaac
     4921 cgatataaaa cagcaggatg cgatacacca cctgatttac cgcttttgga atgctttttt
     4981 ccggatcgcg cgcttcagcg gctgtaatcc caatcagctc cagaccgccg aaggagaaca
     5041 taattaccgc cagcgacaaa atcagcccat tccagccggt ggcgaagaaa ccaccgtagc
     5101 gccagaggtt gtcgatactg gctttctcgc cgccgtgacc agaaaacagc agccacaggc
     5161 caaagccgat cataccgatg atcgccagca ctttaatcag cgcaaaccag aactcggttt
     5221 cgccatataa gcgcacgttc accaggttaa cggcgttgat gataataaag aaggcggcag
     5281 cccaaatcca cgttggaaca tccgggaacc agtactgcat atagatgccc gcagcggtca
     5341 gctctgccat tcccaccagc acgaacatta cccagtagtt ccagccagag aggaagcccg
     5401 caaacggtcc ccagtattta taggcaaagt gggcaaatga accggatacc ggctcttcga
     5461 ccaccatctc gccaagctgg cgcataatca ggaaagcgat gatcccggcg acgccgtagc
     5521 ccagcaatac agccggaccc gccatctgaa tcgccgggcc aatgccaaga aacagaccag
     5581 taccaattgc gccacccagc gcaatcagtt gaatatgacg gttatgtaat ccgcgatgaa
     5641 gcgtcggctc ttgattcgac gcagtatctt ccgatacggt tgacgcgttt ttcacgcctt
     5701 tcccctgtgt gtcttttttg ttgaggggca ccttttaaca tttagtgccc atcgtcgcaa
     5761 gacacaatcc acacggttaa acgaggtatc ctgctttaat tccgtacccg atgtcggtgc
     5821 agccacatca gcttatacgc cgccgggata ataaacagcg acagcaaagg tgcggtgatc
     5881 atgccgccaa tcatcggggc ggcaatccgg ctcatcacct ctgaaccagc ccccgttccc
     5941 cacagaatcg gcagcagacc ggcgataatc accgccaccg tcatcgcttt cgggcgcacg
     6001 cgcaggaccg cgccgtgata caacgcctca tccagcttct gctcgctgaa tgtttgtgga
     6061 ttattcaacg acggctcggc ctctatggcg tgacgtaaat acatcagcat caccacgcca
     6121 aattcggcgg cgaccccggc aagggcaata aagccggtac ccgtcgccac agaaagatga
     6181 aagcccatcc accacagtaa ccagatgccg cccaccagcg caaacggtac gctgctgata
     6241 atcagcaacg cttcgcccac ccgacggaat gccagataca acagcacgaa gatgatcatc
     6301 agtgtcatcg gcaccatgag tttcagctta tggttggcgc gctccagtag ctcgaactgc
     6361 ccggagaatg ccacgctggt gcccggtttt aactgcactt tttcagctat cgctttttgc
     6421 aaatcgtgaa ccaccgacac catgtcacga tcgcgggcat cgatataaat ccagctcgtc
     6481 gggcgcgcat tctcggtttt cagcatcgac ggtccggtag agactttaac gtcggccacg
     6541 tctgccaggg tgatttgctg cttcatcggc gtcaggatcg gcaactggcg cagtgcctgc
     6601 ggactatcgc gccagctttg cggataacgc agattaattg gataacgggc aatcccttcc
     6661 accctttcgc caaccatcgc cccgcccacc gcagaagtca caaacaactg cacatccgcc
     6721 accgtcatac cgtaacgtgc ggctttttca cggttaatct caacgttgat atagcgccca
     6781 ccttccagcc gctcggcaag cgcagaagct acgcctggca ccgttcgcgc cacttcttca
     6841 atttgctcag ccatcgcgtc gatatccgcc agcacagtgc cggaaacttt aatgccgatg
     6901 gggcttttaa tgccggttga gagcatatcg atacggttac gaattggcgg cacccacaga
     6961 ttcgccagcc ccggcagacg cacggtgtta tccagttcct caatgatttt gtccattgtc
     7021 atgcctggcc gccactgatc ctgtggctta agctggatgg tcgtttctac catctccagc
     7081 ggagcagaat cggtggcggt ttccgctttc ccggttttgc caaatacccg cgccacttca
     7141 ggtacgctca taattagctt gtcggttttt tgcagcatac tcgccgcctc tgctgcggaa
     7201 atccccggca gcgtcgatgg catatacaac aagtcgcctt cattgatctg cggtaaaaat
     7261 tccccgccaa ctttattgag cggccagaga accgtcagca ccgaaagcgc cgccaccagc
     7321 agcgtggttt tcggccagtg cagtactttc agcaacagcg gatgataaac acgaatcaaa
     7381 aagcgattga gcggattact gctttccggc ggaattttgc cacggatcca gtaacccatc
     7441 aggataggga tcactacgat cgccagcagc gccgcacccg ccatcgcata cgttttggtg
     7501 aacgccaacg ggccaaacag acgcccttcc tgcccttcca gggtgaagat cgggataaac
     7561 gacaacgtga taatcagcag actgataaac agcgccggcc ccacttcaac agacgcatcg
     7621 gtgatcacct gccagcgcgt tttattatcc agcgtggcgt caggatgctg gtgctgccac
     7681 tcttccagcc gtttatgcgc attctcgatc atgacgatag cggcatcgac catcgccccg
     7741 acggcaatcg caatgccacc cagcgacata atattggcat tcagcccctg gaagggcatg
     7801 acaataaaag caatacacaa ccccagcggc aacgaaataa tcgccaccag cgccgagcgc
     7861 acatgccaga gaaacagcgc gcagactacc gccaccacaa taaactcttc cagcaacttg
     7921 ccgctgaggt tgtcgatggc ccggtcaatg agctggctgc ggtcgtatgt tgtaactatc
     7981 tccacgcctt ccggcagact acttttcagc gtttccagtt tgtccttcac ggcggcgatc
     8041 acttctcggg cgtttttgcc ggatcgcagg atcaccaccc cgcccgccac ttcgccttcg
     8101 ccgtttaatg ccccggcgca tctccgggcc aatctggacc ttcgcaacat cgcgcagata
     8161 aacgggcacg ccattttcac tggcttttaa aacgatgtga ttaaagtcgt cgagcgtttg
     8221 tagatagccg ctggcgcgca ccatatattc tgcttccgcc agttcgattg acgaaccgcc
     8281 cgcttcctgg tttgaagcat ccagcgcgct ttttacttcg gcgagactga tgccatactg
     8341 cgccaggcgc tgaggatcga taaccacctg atactctttc accacaccgc ctaccgacgc
     8401 cacttccgca acgtcaggaa tggttttcag ctcatatttg agaaaccagt cctgcaatga
     8461 gcgtaaatcg gcaagatcgt gcttaccgct gcgatccacc agtgcatatt catagatcca
     8521 gccaacaccc gtggcatctg gtcccagctc ggcgctgact cccgcaggca gcttaccctg
     8581 tacctggttg aggtactcca gcacccgcga acgcgcccag tacggatcgg tgccatcttc
     8641 gaaaatgaca tacacataag agtcgccaaa ctgcgagaaa ccacgcacag tcttcgcgcc
     8701 aggcaccgac aacatggtgg tggttagcgg ataagtcacc tgattttcaa cgatttgcgg
     8761 tgcctggccg ggatagctgg ttttaataat cacctgcaca tcggagagat ccggcaacgc
     8821 atccactggc gtattaatga tggtccaggt gccccagatg ctcagaaaca acgcgcccat
     8881 cagcaccaaa aaacggttcg ccaccgagcg acgaataatc cattcaatca ttggttattc
     8941 cctcaatgcg catgggtagc actttcagag cgcatccgct ccagtgcgcc agaaatattg
     9001 gcttcggaat caatcaggaa caggccgccg gaaaccacct tttcaccttc cgccagacca
     9061 gagcgtaatg cggtgacgcc ttgcgatgcc tggaaaacag caacgcgttt cggtacaaag
     9121 cgcccgtcgg catcaacggt aatcacccgc tgttcgttgc cggtatcaat cagcgcctcg
     9181 tcggcgttgt cgacttccag acgcagctgc agcgtgcggg tcgcggcatc cacgccaggt
     9241 agcagcgtcc atttgcggat ggtgagtgtt ttatccggtc gcgccggaac ggtgagggta
     9301 aactgcgagg catctttcac cagccaggcg atggactccg gg
//
LOCUS       DAJVQH010000139         9241 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJVQH010000139 DAJVQH010000000
VERSION     DAJVQH010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.139"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OQM87_003531"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OQM87_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCS1509522.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OQM87_003532"
     CDS             1828..3147
                     /locus_tag="OQM87_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCS1509523.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3433..3474
                     /locus_tag="OQM87_003533"
     CDS             3433..3474
                     /locus_tag="OQM87_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509524.1"
                     /translation="MVQLVDLARCVSF"
     gene            3512..4657
                     /gene="dosC"
                     /locus_tag="OQM87_003534"
     CDS             3512..4657
                     /gene="dosC"
                     /locus_tag="OQM87_003534"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCS1509525.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4582..5199
                     /locus_tag="OQM87_003535"
                     /pseudo
     CDS             4582..5199
                     /locus_tag="OQM87_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5457..5789
                     /gene="ddpX"
                     /locus_tag="OQM87_003536"
                     /pseudo
     CDS             5457..5789
                     /gene="ddpX"
                     /locus_tag="OQM87_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5803..6699
                     /locus_tag="OQM87_003537"
     CDS             5803..6699
                     /locus_tag="OQM87_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCS1509526.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6696..7682
                     /locus_tag="OQM87_003538"
     CDS             6696..7682
                     /locus_tag="OQM87_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1509527.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OQM87_003539"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OQM87_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OQM87_003540"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OQM87_003540"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCS1509528.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2256 a         2256 c         2321 g         2408 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acacttagtt aaacagctat aaatccctaa taaaaatgta tgaatatgaa
     3301 aaaccatacg atattcgctg tcaccgaaaa ataattacga tgggctatac tcttctgaag
     3361 aacagtagcg tagattgaaa ctgtcttaag tcgcttattt cttttcagta tatcttcata
     3421 tttcaggaga atatggtgca gttagtcgat ctcgcacgtt gcgtttcgtt ttgattccgt
     3481 ctgatttccc agccttataa gggtgtgaat tatggagatg tattttaaaa gaatgaaaga
     3541 tgagtggacc ggacttgtcg aacaggcaga tcctctcatt cgtgctaaag ccgcagaaat
     3601 tgcccttgcg catgctcatt atctgagtat cgagttttat cgaattgtcc gcatcgaccc
     3661 gcatgccgaa gaattcttga gtaatgaaca agttgagcgg cagttgaaaa gtgcgatgga
     3721 acgctggatt attaacgtgc tttctgccca ggttgacgat gtcgaaaggc taatacaaat
     3781 ccagcatacc gtcgcggaag tgcatgcccg cataggaatt ccggtagaaa ttgtcgagat
     3841 ggggtttcgg gtgctgaaaa aaatcctcta tccggtaatc ttctcttcgg attattccgc
     3901 cgcagaaaaa cttcaggtct accatttctc gattaacagt attgatatcg cgatggaagt
     3961 gatgacccgc gcgtttacct ttagtgacag tagtgcctca aaggaagatg aaaactatcg
     4021 tatcttctcg ttactggaaa acgccgaaga agaaaaagaa cggcaaatag cctcaatact
     4081 ttcatgggaa atagatatta tctataaagt cctgctggat tctgatttag gcagcagttt
     4141 gcctttaagc caggctgatt ttggcctgtg gtttaaccat aaaggtcgac attattttag
     4201 cggcattgcc gaagtgggcc atatctcccg tctgattcag gatttcgacg gtattttcaa
     4261 tcaaaccatg cgtaacacca ggattttgaa taacagaagt ttgcgggtga aatttctatt
     4321 acagataaga aataccgtat cgcagattat taccttgctg cgtgaattgt ttgaagaagt
     4381 atcgcgccac gaagtcggta tggatgtact gacgaaatta cttaaccgcc gtttcctgcc
     4441 gactatcttc aaacgcgaaa ttgcccatgc caaccggacc ggtacaccgc tgtcagtgct
     4501 gattattgac gttgataaat tcaaagagat caacgatacg tggggccata acactggcga
     4561 tgaaattctg cgtaaagtct cattcctctc gcagaagaga ttggtgaaat cgaaaatatt
     4621 gggcgctggg tcatcgcgga agcttgccgt cagttagcag aatggcgtag ccagaatatt
     4681 catatcccgg cgttatccgt gaacttgtcg gcgctgcact ttcgcagtaa tcagctgcct
     4741 aatcaggtgt ctgatgcaat gcaagcctgg ggtattgacg gccaccagct gacggtggaa
     4801 atcacggaaa gcatgatgat ggaacacgat accgaaatct ttaagcgcat tcagatcctg
     4861 cgtgatatgg gcgtgggttt atcggtagat gattttggca cgggcttttc cggattatcc
     4921 cgcttagtca gtcttccggt aacggaaatc aaaattgaca aaagctttgt cgatcgttgt
     4981 ctgacggaaa aacgcatcct ggccttactt gaagccatta ccagcattgg gcaaagcctc
     5041 aatttaaccg tcgtggcgga aggggtcgaa accaaagagc aatttgagat gctacgtaag
     5101 atccactgtc gcgttattca gggatatttc ttttcccgcc cccttcccgc cgaagaaatt
     5161 ccaggctgga tgagcagcgt gttaccgctg aaaatctgac aaattcctct cgcccgcact
     5221 cgcgggtttt cctttaaggt gacactgtca cttaaatact gtgatttcag ctacgattcc
     5281 gggagattcc ttccttaact ttccctcttg caccaaaact gcactacgct gcacaaatgt
     5341 tgatcaaaag ttaaaaaata taactttaag ccagcattct ggcgcttatc ccggcatggc
     5401 atgagatctg cataagcgga aagcgcagca atttttgtct tatacaggaa ccgtttatgt
     5461 cggataccac cgaactggtt gatttagccg tgatcttccc tgatctggag atcgaattga
     5521 aatatgcctg cgttgataac atcacaggta aagctattta tcagcaagcg cgttgtctgc
     5581 tacataaaga tgcgattacc gcgctggcga aaagtatcag tatcgcccag ctgtcaggct
     5641 tacaactggt gatttacgat gcgtatcgcc cacaacaagc acaggcgatg ttgtggcaag
     5701 cctgcccaga cccgcaatat gttgttgatg tgacggtcgg ttctaatcac agccgtggca
     5761 cggcgctcga cctgacgctt cgtgatgacg tggaggtggt gaatgatgct aagcgaggaa
     5821 acgtccgccg tacgcccaca aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg
     5881 ctgaaaggca gtccgctcac cgtgaccggc gcagtcatca ttgtattaat gcgactgatg
     5941 atgatttttt caccgtggct ggcgacgcat gatcccaacg ccattgattt aaccgcccgc
     6001 cttttgccgc cttctgcggc gcactggttt ggcaccgatg aagtgggacg cgatctgttt
     6061 agccgcgtac tggtcggcag tcagcaatca attctcgccg gattagtggt ggtcgccatt
     6121 gcgggtatga ttggttcgct actcggatgt ctatccggtg tgcttggcgg acgcgcagac
     6181 gccatcatca tgcgcatcat ggacattatg ctgtcgattc cttcgttggt actgacaatg
     6241 gcactggctg ccgctctcgg gccgagttta tttaacgcca tgctggcgat tgctattgtg
     6301 cgaattccct tttatgtgcg cctggcgcgg gggcaagcat tagttgtacg ccagtatacc
     6361 tatgttcagg cggcgaaaac ctttggcgcg tctcgttggc atctgatcaa ctggcatatt
     6421 ttacgtaact ccctaccgcc gctgatcgtg caggcatcgc tggatatcgg tagcgcgatt
     6481 ttaatggccg ccacgttggg atttattggc ctgggtgctc aacaaccgag tgctgaatgg
     6541 ggcgcgatgg tagcgaatgg tcgcaactat gtactcgatc agtggtggta ttgcgcattt
     6601 ccgggggcag cgattttgct taccgccgtc gggtttaatc tctttggtga tggtattcgc
     6661 gatctgcttg acccgaaagc aggaggaaag cagtcatgac ccaacccgtt ctggacattc
     6721 aacagctgca tttgagtttc cccggtttta acggtgatgt tcacgcgctc aacaatgtgt
     6781 ccttgcagat taaccgcggc gaaattgtcg gtctggtggg agaatccggc tcaggtaaat
     6841 cagtcaccgc aatgctgatt atgcgtctgc taccgacggg cagttattgc gtacatcggg
     6901 ggcagatttc actgctggga gaagatgttc ttaacgctcg ggaaaaacag cttcgtcagt
     6961 ggcgcggcgc acgagtggcg atgatctttc aggaaccgat gaccgccctt aatccgacac
     7021 gtcgaatagg tcttcagatg atggacgtaa tccgccatca tcaaccaata agtcgtcggg
     7081 aagccagagc taaagcgatt gccctgctgg aagagatgca aatcccggat gccgtggaag
     7141 ttatgtcgcg ctatccgttt gagctttcag gtggtatgcg ccagcgggta atgattgcgc
     7201 tggcattctc ctgcgagccg caattgatta ttgccgacga accgacgacg gcgctggacg
     7261 tcacagtaca gttgcaggta ctgcgtttgc ttaaacataa agcacgcgcc agtggaactg
     7321 cggtactgtt catcagccat gatatggccg tggtgtcgca actgtgcgat agcgtttacg
     7381 tgatgtatgc cggaagcgta attgaaagcg gcgggacggc agacgttatc catcatcccc
     7441 ggcatccgta taccattggt ttgctgcaat gcgcaccaga acatggagta ccacgccagc
     7501 cattacccgc cattccaggg acggtaccaa acctcaccca tttgcctgac ggctgcgctt
     7561 ttcgcgatcg ttgctatgcg gcaggtgcac agtgtgaaaa cgtcccggcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcgcctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggtaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttactgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gagctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgagctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
     9241 a
//
LOCUS       DAJVQH010000140         9150 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJVQH010000140 DAJVQH010000000
VERSION     DAJVQH010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.140"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..914
                     /locus_tag="OQM87_003541"
     CDS             <1..914
                     /locus_tag="OQM87_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1509529.1"
                     /translation="EAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPPALV
                     LMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPVKPRPQLQF
                     SEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIPFLA
                     AAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVAFIS
                     MALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTTHSF
                     NLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1166..2083
                     /gene="oxyR"
                     /locus_tag="OQM87_003542"
     CDS             1166..2083
                     /gene="oxyR"
                     /locus_tag="OQM87_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCS1509530.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2066..3466)
                     /gene="sthA"
                     /locus_tag="OQM87_003543"
     CDS             complement(2066..3466)
                     /gene="sthA"
                     /locus_tag="OQM87_003543"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCS1509531.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3787..4437
                     /gene="fabR"
                     /locus_tag="OQM87_003544"
     CDS             3787..4437
                     /gene="fabR"
                     /locus_tag="OQM87_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCS1509532.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4437..4796
                     /locus_tag="OQM87_003545"
     CDS             4437..4796
                     /locus_tag="OQM87_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCS1509533.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4836..5936)
                     /gene="trmA"
                     /locus_tag="OQM87_003546"
     CDS             complement(4836..5936)
                     /gene="trmA"
                     /locus_tag="OQM87_003546"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCS1509534.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6050..6240
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6305..8149
                     /gene="btuB"
                     /locus_tag="OQM87_003547"
     CDS             6305..8149
                     /gene="btuB"
                     /locus_tag="OQM87_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCS1509535.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALDHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8094..8951
                     /gene="murI"
                     /locus_tag="OQM87_003548"
     CDS             8094..8951
                     /gene="murI"
                     /locus_tag="OQM87_003548"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1509536.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2093 a         2218 c         2465 g         2374 t
ORIGIN      
        1 gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactggtat gcacaaaaag
       61 aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag gctatcgcgc
      121 ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc gttatcatcg
      181 gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac cgggcgcagt
      241 ttgtcctgac tgacgaagaa cggacgtatc tctccgcgcc ggttaaaccg cgtccacagc
      301 tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg atgattttag
      361 gtttctctgg cgtcaattat accggttggc tctacattgc gtggttaccc ggttatttgc
      421 aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt cctttcctcg
      481 cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg aaaaaaggct
      541 acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg tcggcattag
      601 gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt atctcaatgg
      661 cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag gtgatggtgt
      721 cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt gtttttgctt
      781 cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt aatctggcgc
      841 tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt attgtcaaag
      901 atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag aataaaaaaa
      961 agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag ttgagaaact
     1021 ctcgaaacgg gcagtgactt caagggttaa aagaggtgcc gctccgtttc tgtgagcaat
     1081 tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta tcgccatgaa
     1141 ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc tggtggcatt
     1201 ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc agccgacgct
     1261 tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg agcggaccag
     1321 ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc gtaccgtgct
     1381 gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga tgtccggacc
     1441 gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata ttatcccgat
     1501 gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga cccaccagtt
     1561 actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg tgaaagagag
     1621 cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta tctatgaaga
     1681 tcacccgtgg gcgaaccgcg aatgcgtact gatggccgat ctggcagggg aaaaactgct
     1741 gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct ttgaagccgg
     1801 ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca acatggtggc
     1861 ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc gcaaacgcga
     1921 tggggttgtt tatctgccat gcattaagcc ggaaccacgc cgcactattg gcctggttta
     1981 tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca tccgcgcaag
     2041 aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt aacgcagcta
     2101 cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag tactcaatag
     2161 tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt tcggcagcgc
     2221 gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac aaaattttca
     2281 gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac tgggcgcggc
     2341 ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc acagagctga
     2401 tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg gtggcttcgc
     2461 cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat gccaggctcg
     2521 gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga tacatgctgt
     2581 tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac gccagcgaat
     2641 cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt tttttacccg
     2701 acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac tcttcgttgt
     2761 gacgaatcac tacgccactg ttccagaagt gataggagag agaatctgac atctcttgat
     2821 cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca tccataccgc
     2881 ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt acatggcgcg
     2941 gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg gtgaaatcaa
     3001 catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca gcggttagtg
     3061 tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca aagcgagcgt
     3121 tcccctgcaa tattttacag tgattacgtt cgtaaaatcc ctgacgcatg cgcgtttgtt
     3181 gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg agcagtcggg
     3241 aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg gcgtgacgga
     3301 gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt tgataacgct
     3361 cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct tcgccgccgg
     3421 ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta gggcttacct
     3481 gttcttatac ataaaatcaa cataatggta acattctatc gcgggtaagc caattgatcc
     3541 ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct tacttttgcc
     3601 ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag gtttccgctg
     3661 tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt cacaatactg
     3721 gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg caaggattca
     3781 gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc gctggtggaa
     3841 gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt gcgtgaagtg
     3901 gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga cgtagacgaa
     3961 ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat gcgccaggca
     4021 cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac atttatggag
     4081 ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc cggcacctcc
     4141 gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga acttgcggac
     4201 tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc cgaagcaatg
     4261 gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga acaacgtcgg
     4321 caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc ttattactgg
     4381 tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga cgagtaatga
     4441 aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggc ttatcgatta
     4501 atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc ccgattattt
     4561 ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc atgtcggtag
     4621 gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac agcacggtag
     4681 ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc tccgtcatta
     4741 tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct gagtaatcgg
     4801 tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag taatacgccg
     4861 cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc gaccttgtgc
     4921 gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga gatatacaga
     4981 atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct gcgcggtggg
     5041 tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag gcggttaaac
     5101 tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat aatttgtacg
     5161 ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg tttggcgatt
     5221 tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt accgttgccg
     5281 cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc ccattccagc
     5341 atttgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg acgatagatc
     5401 atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag cgcgattttg
     5461 gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag tgcatcgcgc
     5521 agtgcttccg cttcctgacg ccactcatca tccagctttt tatggtacag cagggaaacc
     5581 accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg gcgcagaacg
     5641 ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag ttcactggcg
     5701 gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat gatgtgatac
     5761 aggtcatcgc catcgtgcca gatgcggaac tccgcgcgca tccggtaatg actgaccggc
     5821 gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg caaacgtacc
     5881 actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg ggtcatgatg
     5941 tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat gtccagatct
     6001 tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc tgtagcatcc
     6061 acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctagagctg acgcgcagcg
     6121 gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg aagtcatcat
     6181 ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg tcgcatctgg
     6241 ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt gtggatgctt
     6301 tacaatgatt aaaaaagctt cgttgctgac ggcgtgttcc gtcacggcat tttccgcttg
     6361 ggcacaggat accagcccgg atactctcgt cgttactgct atccgttttg aacagccgcg
     6421 cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc gctggcagtc
     6481 gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc aaaacggcgg
     6541 ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg tgctggtgtt
     6601 aattgatggc gtccgcctga atctggcggg ggtgagtggt tctgccgacc ttagccagtt
     6661 ccctattgcg cttgtccagc gtattgaata tatccgtgga ccgcgctccg ctgtttatgg
     6721 ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac ccggaacgga
     6781 aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta cgcagcaaca
     6841 actgggggat aaaacacggg taacgctgtt gggcgattat gcccatactc atggttatga
     6901 tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg gttttttaag
     6961 taaaacgctt tatggggcgc tggatcataa ctgtactgat gcctggagcg gctttgttcg
     7021 cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg gttcaccgtt
     7081 gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct ataacggcga
     7141 actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca actacgatcc
     7201 ccattatggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat acaccgtcca
     7261 gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg actggcagaa
     7321 acagactacg acgccgggta caggttatgt tgaggatgga tatgatcaac gtaataccgg
     7381 catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg cagcacgcag
     7441 cgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg gttgggaatt
     7501 catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac caaatctggg
     7561 gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca aacagtggga
     7621 aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat atcgtaacga
     7681 tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg aagggaaagc
     7741 gcggattaag ggcgtcgagg cgacagccaa ttttgatacc ggaccactga cgcatactgt
     7801 gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt tacgccgtgc
     7861 taaacagcag gtgaaatacc agctcgactg gcagttgtat gacttcgact ggggtattac
     7921 ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt atcaaaccgt
     7981 taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca cctctcacct
     8041 gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag tctatggcta
     8101 ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa ccacgtccca
     8161 ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag atccggcatc
     8221 tcttaccgga tctccattac atttatgctt tcgataacgt cgctttcccg tatggcgaaa
     8281 aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt caccgcggtg caagaacgtt
     8341 atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca cttcctgcat
     8401 tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa cctgctgcac
     8461 gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaacagtt aaacgttctt
     8521 atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg ctgggctcgg
     8581 cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct ctggatgcac
     8641 taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc gttgtattgg
     8701 gttgcaccca tttccctcta ctacaagaag aactgttaca agtgctgcca gagggaaccc
     8761 ggctggtgga ttctggcgca gcgattgctc gccgaacggc ttggttgtta gaacatgaag
     8821 ccccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg acgccagaag
     8881 ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa aaactggcag
     8941 ttttaggctg atttggttga atgttgcgcg gtcggaaaat tattttaaat ttcctcttgt
     9001 caggccggaa taactcccta taatgcgcca ccactgacac ggaacaacgg caaacacgcc
     9061 gccgggtcag cggggttctc ctgagaattc cggcagagaa agcaaaaata aatgcttgac
     9121 tctgtagcgg gaaagcgtat tatgcacacc
//
LOCUS       DAJVQH010000141         8899 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJVQH010000141 DAJVQH010000000
VERSION     DAJVQH010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.141"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..2886
                     /locus_tag="OQM87_003549"
                     /pseudo
     CDS             1..2886
                     /locus_tag="OQM87_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(2996..3844)
                     /gene="alkA"
                     /locus_tag="OQM87_003550"
     CDS             complement(2996..3844)
                     /gene="alkA"
                     /locus_tag="OQM87_003550"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCS1509537.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            3978..5330
                     /gene="yegD"
                     /locus_tag="OQM87_003551"
     CDS             3978..5330
                     /gene="yegD"
                     /locus_tag="OQM87_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1509538.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5342..7282)
                     /gene="yegI"
                     /locus_tag="OQM87_003552"
                     /pseudo
     CDS             complement(5342..7282)
                     /gene="yegI"
                     /locus_tag="OQM87_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7279..8040)
                     /locus_tag="OQM87_003553"
     CDS             complement(7279..8040)
                     /locus_tag="OQM87_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCS1509539.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(8037..8696)
                     /locus_tag="OQM87_003554"
     CDS             complement(8037..8696)
                     /locus_tag="OQM87_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1509540.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2169 a         2360 c         2323 g         2047 t
ORIGIN      
        1 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
       61 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
      121 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
      181 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
      241 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
      301 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
      361 cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
      421 tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
      481 cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
      541 gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt caggaagagc
      601 tgtgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
      661 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
      721 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
      781 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
      841 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
      901 tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
      961 atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     1021 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaagctgga
     1081 atttcgcatt gccgtgaaag atggcatccg ccatatccgc gccctcgcca accgggtact
     1141 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ccgaggttaa
     1201 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc tggattccat
     1261 cggtgaagcc gtggtctgta ttgatatggc gatgaaaatt acctttatga atccagcggc
     1321 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     1381 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
     1441 acgttccgcg attgaacaag atgtggtgtt gcactgccgg aacggcggca gttacgacgt
     1501 gcattacagt attacgccgt taagtactct ggacggcagc aatataggct ctgtactggt
     1561 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     1621 tgatgcactg acgcatctcg ccaatcgcgc caattttgag aagcaactgc gtatcctgct
     1681 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     1741 taaagcggtg aatgacagcg ccgggcatgc ggcgggtgac gctttactgc gcgaactggc
     1801 gtcgttgatg ctgagtatgc tgcgctccag cgacgtgctg gcgcgactcg gtggtgatga
     1861 atttggtcta ctgttgccag actgtaatat cgaaagtgcg cgttttatcg ctacacgcat
     1921 tatcagtgcc gtgaatgatt atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     1981 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     2041 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggttacgaa
     2101 ccacagcaag ctgccgcaca tagcgaacgg gcggcgatgt cgcttgatga acagtggcgg
     2161 atgattaaag agaatcagtt gatgatgatc gcccacggtg tcgcttcgcc gcggatcccg
     2221 gaagcgcgta atttgtggct gatttcactt aagctctgga gttgcgaagg cgagattatt
     2281 gatgaacaaa aatttcgtcg tagcttcagc gatccggcac ttagccatgc tcttgaccga
     2341 cgggtattcc acgaattttt ccagcaggcc gcaaaagcgg ttgccagtaa aggcataagc
     2401 atcgccctcc ccctttccgt tgccggtttg agtagcgcca cgctggtgaa tgatctgctt
     2461 gagcagctgg aaaatagccc tctaccacca cggttattac atctgattat tccggctgaa
     2521 gcgattttag atcacgcaga aagcgtgcaa aaactgcggc tggcgggatg tcggatagtg
     2581 ctcagccagg tgggccgcga tctgcaaatc ttcaactcgc tgaaagcgaa tatggcagat
     2641 tacctgctac ttgatggtga gttatgcgcc aacgtgcagg gtaatttgat ggatgagatg
     2701 ctgattacga ttattcaggg gcacgctcag cgactcggga tgaaaaccat cgccgggcca
     2761 gtcgttttac ccttagtgat ggatacgctt tctggcatcg gcgtcgatct gatttatggt
     2821 gatgtgattg ccgatgccca accgctggat ttgctggtga atagcagtta tttcgcgatt
     2881 aactgagaga gcgtttgccc attgcctgat gcgatgctaa cgcgtcttat caggcctaca
     2941 aatcgctcat tccgcaggcg ggataaggcg ctcgcaccgc ttccggcgac caacgtcatg
     3001 cttcgtctgg ttgccagcct tccgtatacc agatatgcaa cagcgcataa gaacgccagg
     3061 gcttccagcg ctcggcataa cggcggattt gtgccggtgt catccccaga aatcgctgtt
     3121 taatcagata atcatccggc agaaaaacat ctttcgcctg ccagccgcgc aaggcgaaat
     3181 aattcgccgt ccagcgcccg atacccggaa aagtttgcag cgttttcatc gcctgctcca
     3241 catcgcccgg tattgtcatt ggtaaggtgc cctccagcgc cgcatttgcc agatgaatca
     3301 gcgcctctgc ccgtttcaac ggcataccta acgcttttaa tgcctgcggg tcggctgctg
     3361 ccagccgctg aggcgtgggg aagcagatat actccggaaa atcatccagc cgttcgccat
     3421 aaagctgtgc cactctggcg gtcaattttg ccgccatcgc cacgctcacc agttggccta
     3481 aaatcgcccg cacgccctgc tcaaaagcat caacactgcc gggtaaacgc aatccgggcc
     3541 gcgccgcgcc taacttgccc aacgcaccgt taacaatctg cgggttacat tgcagatcaa
     3601 gcaggcggct cattttcgcc agacactcag cggcaacggg ttctaaacct gcacttaaat
     3661 ttatgtgcag agtatggcgg gctatatccg gaatagcagt caccacgccg cgatattcgc
     3721 ccactgccag actacgggca taataactgt ccgcgaccgt ttccacacca ctcaccgcac
     3781 gggcggcgag aaatcccaac atccacgacc agtcatacgg cggctgccag ttcaggatat
     3841 acatcgcatc tccttattca tccgctttca gcataaacgt tattcagacg ttacgctttg
     3901 ctttcatatt ccggttgtcg cgacggcaac atttcgctaa agtcacgccc cttcttcacc
     3961 ggcatgggga ttatttcgtg tttattggtt ttgattacgg tacagcaaac tgttcagtgg
     4021 cggtcatgcg tgacggtaaa ccgcatttgc taaaaatgga aaacgacagc acgctgctgc
     4081 cttcaatgct ttgcgcgcca acgcgtgaag cggtaagcga atggctgtac cgccatcatg
     4141 acgttccggc agacgacgat gaaacgcagg cgttgttacg acgggcgatt cgttataacc
     4201 gcgaagaaga tatcgatgtt acagcgaaaa gcgtgcagtt cggtctttcc tcactggcac
     4261 agtacattga tgatccagaa gaagtgtggt ttgtgaaatc accaaaatcg ttcctcggtg
     4321 ccagcggctt aaaaccgcag caggtagcgc tgtttgagga tctggtctgc gcaatgatgt
     4381 tgcacattcg ccagcaggcg caggcgcagc tgccagaagc aattactcag gcggtgattg
     4441 gtcgcccgat caacttccag gggctgggcg gcgatgaagc aaacgcccag gcgcagggga
     4501 ttctggaacg cgcggcgaag cgtgccgggt ttaaggatgt ggtattccag tacgagccgg
     4561 tagcggctgg gctggattac gaagccacct tgcaggaaga aaaacgggtg ctggtggtgg
     4621 atatcggcgg tggtacgact gactgttcat tgctgctgat ggggccgcag tggcgttcgc
     4681 gtctcgatcg tgaagccagt ctgctgggtc acagtggttg tcgtattggc ggtaacgatc
     4741 tggatatcgc cctagcgttt aaaaacctga tgccactgct gggtatgggt ggcgaaaccg
     4801 aaaaaggcat cgccctgccg atcctgccgt ggtggaatgc ggttgccatc aacgacgttc
     4861 ctgcacagag tgatttctac agtagtgcca acggtcgtct gcttaacgat ctggtacgcg
     4921 atgcccgcga accggaaaaa gtggccctgt tacagaaagt ctggcgtcag cgtttaagct
     4981 atcgcctggt gcgtagcgca gaagagtgca aaattgctct ttcaagcgta gcggaaaccc
     5041 gcgcctcact gccgtttatc agcaatgaac tggctacgct gattagccag cgagggctgg
     5101 aaagcgccct cagccagcca ctggcgcgga ttctggaaca ggtgcaactg gccctggata
     5161 acgcccagga aaaaccggac gttatctacc tgaccggcgg tagcgcccgt tctccgctga
     5221 ttaaaaaagc gctggcagaa cagttgccgg gcattccgat tgcaggcggc gatgactttg
     5281 gctccgtcac cgccgggctg gcacgctggg cggaagtggt atttcgttaa aagaaaatgg
     5341 ctcagcagcg gcttaaatca gcctgcgcct gagccagttt ttctgccgcc tgacgtaacg
     5401 gttccatcaa cggcatggtc cgtgctggag catgaaggcg gaatcgctgc aactctgtcg
     5461 cgccgacagt caacgtcgat tccagccgat gtcgtttggc ggccattttc gccattaccg
     5521 cctgtctgtc tgctggcgtt accgcttcgt tcggcctgaa cacaaaacgg cgttcacagc
     5581 tcgctttcca gtcgatgacc gcctgggtca gatgatcacc aaacccttta acttgcttaa
     5641 cgccacgacg ggtaacacct gctgctgttt caataccaaa agaccataac gccgctttac
     5701 gcgcagggcc aacgccggga atagaggcaa catcaataaa aaatccctcc agaaacttct
     5761 acttctgccg ttcccttgca gtgtcatgaa gtgctgccag agcgagtttc tcttcttccg
     5821 gtaacccgag aatttcgtcc ttcatttttt cgagcatcgt ccgtttggcg ataaaacctt
     5881 ccaggccgcc tacctgctgg atctggcgga ctaaatggtc atagtccatt ttcgcacgat
     5941 taaatgcctc acggcgttgc tgaacttctg ctttgtacgc tttgcttgtc agactgccaa
     6001 taatccagat agccgccagc acaggaacca aaataatata acgcggttct gcctgaaggc
     6061 cgcaaagcag cagcgataat gctgacagtg cgatctcaag cagaatgatg tattcgcgcc
     6121 gtaacaggcc taaaggaagc ggcctgccag tcggttggaa atgatcgggt aatggcagtt
     6181 gcaatgctgg cggtgctact gacgccatca ccatcgccca gacttttgcc agcacaaaat
     6241 ccccgccggt ggtaatgacc tcttcgccga gatcaataaa atagataacg ccttgattat
     6301 ccagcgtaca ccacaggcag tcggtcaaat gagcgggata aacatgcatt gccgaaacgg
     6361 gacatttctt taactgttgg cgcagagaat caagcgccgc tacccacgct ttagccgtcg
     6421 gacgcccggt tgccacgcca ctttccgtga atgcctgctg aaacatggct tcaacatcac
     6481 tcggtaacat cgatagcgga atcgatcgtg gcggcggttt taaaccacgt cgctgattat
     6541 ctgacgcgta ggcataacgg aaatgggtaa tatccgtctc cagcgcatta cccgcatcag
     6601 agataagcgg cacaccagaa taaggatgcc ggccaccaaa caagacgtga aaaatcagca
     6661 acgcaaggcc aaaattaccg tgattttctg tgcgttcaaa gccgacaaat gacgacaagg
     6721 tttgtagctc tggcggcgta aaatgcgaca cgccgacttc gcataaatgc agtgtgccat
     6781 tggcgttaat ctgaaaggag tcgctatcaa tcaacaccac tttgctgtcg cgacctacca
     6841 taaaactgtt ctggtttacg tcacccacga catgcccgtg ctcgtgaacc gtagcaaaag
     6901 atgaagcaat attgcgcgca acatagagta gaaaatccca cgcacaatga gggtaacgtt
     6961 gacgacgatg tgccgggcta tagatcatat gaatcggttc tttaccagaa acttttggca
     7021 tcataaaacc gataactttt ccgcctcgtc caccgtgaag cgttgcctgc ggccaggcga
     7081 cataattcaa caattgcgcg tcagctgtcg cagccataaa ggcaagtttg tcctgtttta
     7141 aggcgggtgg cggcgtgtga taaatcttgg cgacgctatc gacaaactca ttgatatcat
     7201 aaaccgcgcc ttcaccgcct ttgcccagtt cacggcctgg cgtaacgcac tcgccagtag
     7261 cagtatataa agtgggtttc atggtaacca cagcgccagt gccaacgttt tatcatcatc
     7321 cgtacgctcg tttaccgccg ggctggacaa aaactgcttt aacaattcag gtaataaatc
     7381 gagttgttcc tgcgttgctg acgccagtcc attgaaaaac ggggtaaaga aaggcacatg
     7441 cggagaatta tccaacatat ttaacgccag tcgctggata ccatccgtaa atgctgcaac
     7501 tttatgcaca cgctcagtgc tggtgaaagt ttccagtctg gaaacggcat cttcatcggt
     7561 aatgaagtgc gtcatattgg catattcacc caccatcggc gtaagcggca attgcagacc
     7621 gtgaccaaaa tccaccacca cgccgccatc gccaatctgc ataatcaagg tgccattagc
     7681 cgatgaaatc aatcccagaa acgtgcaggc aaaatcgcga actgctaatt ctttggcttc
     7741 cgcctcggca aaaagtcgct gacgaacggt cagcacaata tccgtggcga ggatatcatt
     7801 aagccccaat tccccaccct gcactttttg cgacatataa gccatcgctt cattgacggc
     7861 gagcatcgcg ccttcgcccc cctgcgaaac gctacctgcg ccatcagcaa ggaacatcac
     7921 cagtaatggc tgctggtcat ttagccaggc aacctgcatc tggcaggcat cctggcaagg
     7981 caagtccgcg ctgatgtgcg acgttccaac agcggatgca taaaccagac gccagctcac
     8041 actgacgtcc agccttttgg cgcttccagc acaacttccg ttcccggcgt ggagcgggaa
     8101 accgaacgca gcgaactgga taaccagcta aacagttcac ggaattgtaa tccttgcaga
     8161 ggcaaaggct gacgaacgct gatttgtgcc agagtcttca tatccgcccc ctgaacaccg
     8221 atggaaaaga aagcaaacct tttatcttct tccccccgaa acactttgtt ggctgcagcc
     8281 tgccactcat cggttggtgc accatcggta atcaggaaaa tccacgggcg ataatacgaa
     8341 ataccatttg cacgatactc acgtttccgc tcctctacca tatccagggc tttggtaatg
     8401 gccgcaccca ttggtgtatc gccctgggca aacaggatgg gcggaaaaaa attagcggcg
     8461 ctggtaaagg gttgttccac atgcaccggg ccgaacgtca caatgccgag ttccactctt
     8521 tttaaagcca gcgaatcggc aagcagctca tcgcgaaagg taaccaatcc ggtgttaagt
     8581 tcattaatag gtctgccact catcgagcca gaaacatcca gcagcagaat gcaggggcaa
     8641 cgtggttccg ggttactggc aaaatcgctg gtggcaaacg tgatttgttc gctcataaga
     8701 aatcctttct tagagaatta ttgaggtccg gtatcatacc gactgcaata atgaaataag
     8761 agcaaacgaa gaaactttca gaattagtcc cggcggcgca gaaaattttg ctattcgcct
     8821 cgcaagaacg gagagccgtc gattgacact aagggcggag tgacataatt tcaggagtga
     8881 gggttaggga gaggtttcc
//
LOCUS       DAJVQH010000142         8761 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJVQH010000142 DAJVQH010000000
VERSION     DAJVQH010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.142"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..366)
                     /locus_tag="OQM87_003555"
     CDS             complement(<1..366)
                     /locus_tag="OQM87_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1509541.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLIIKNSTIHGMIYSECMTT"
     gene            890..1864
                     /gene="hemB"
                     /locus_tag="OQM87_003556"
     CDS             890..1864
                     /gene="hemB"
                     /locus_tag="OQM87_003556"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCS1509542.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1970..2821)
                     /gene="tauD"
                     /locus_tag="OQM87_003557"
                     /pseudo
     CDS             complement(1970..2821)
                     /gene="tauD"
                     /locus_tag="OQM87_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2806..3645)
                     /gene="tauC"
                     /locus_tag="OQM87_003558"
     CDS             complement(2806..3645)
                     /gene="tauC"
                     /locus_tag="OQM87_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCS1509543.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAVTRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQW
                     VNV"
     gene            complement(3642..4409)
                     /gene="tauB"
                     /locus_tag="OQM87_003559"
     CDS             complement(3642..4409)
                     /gene="tauB"
                     /locus_tag="OQM87_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCS1509544.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4422..5348)
                     /gene="tauA"
                     /locus_tag="OQM87_003560"
     CDS             complement(4422..5348)
                     /gene="tauA"
                     /locus_tag="OQM87_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1509545.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDDKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            complement(5615..6130)
                     /locus_tag="OQM87_003561"
     CDS             complement(5615..6130)
                     /locus_tag="OQM87_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509546.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARACGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQKTQSDLSQGDQLTEMLSFLTSEER
                     EALTNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEAS
                     ILSESVNAKAG"
     gene            6323..6574
                     /locus_tag="OQM87_003562"
     CDS             6323..6574
                     /locus_tag="OQM87_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1509547.1"
                     /translation="MRQREDSGRDWPSLMIQQALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            6576..8669
                     /locus_tag="OQM87_003563"
     CDS             6576..8669
                     /locus_tag="OQM87_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1509548.1"
                     /translation="MQSYVTVNDLLGVPGMPATAKGIRQALQRFSRALGDVSRRRAGT
                     KAIEYHIDCLPEVTQKALRERYVEQLVATENNVSEVKTVTRTARNPDAVQAVEAYRGS
                     PQLMEERLNALTENQRRVSEARAALAVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAINANAKRGTNRTISRDPLYQWVLKYNRSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQANGRPMSEAYEDFVTEWQRRYADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEIV
                     GILPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRETLRVLNRD
                     LRSAFNALQQDKPLNARQKSAMRELPSWSELIEAIREGVEWYNNRPHSELPMKPNGKH
                     YSPAEFRKKRLAEENTEIEWLSDLELRDMFCPMVERPVRRCEIKWLGNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFPVSADYYKQQQRIKGMHK
                     RAEEKIRDAVDEGIQVIEQKTTEPWLDNVYRPVGNVMTVQQQEYEEEHDEESERNFRR
                     GLQKLIAMQDDDPLA"
BASE COUNT         2228 a         2254 c         2201 g         2078 t
ORIGIN      
        1 agttgtcatg cactcggaat aaatcatacc gtgaatagtg ctgtttttga ttatcagact
       61 attggcgttg gtattggtag aaataccgtc gtccagatag taggtagaaa tcacgccgtt
      121 aacggtagcg ttattaatga ccgggtagat atcgccgttg taatagctat cagcggcata
      181 gttgttccag cctacataac catcatagta aacgtcatca gcataggtgg cgtcgttgta
      241 gtgatggaaa gtattgtaag tttgaccaga aatatcgttg gtcgctgcat tagcctgaga
      301 ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga
      361 gtgcatttgt catccccctc agggacgtaa taagttgatc catcaattat caatgcatag
      421 aaaacatatt atgtttcgcg ctaacgatta tgctgcgtta cagacgaatg gttcaattat
      481 tctttcccct aagtccgaat gtggacatat ataatttatg gagaaaaaca cgcatcaaat
      541 tgataaaagg acgatatttc agcatgttgc agataatgtt atttttgatg catacgcgtt
      601 ataaatatca aaaacacaaa ccagataaat atcttatcaa tatacaacaa agtttgaatt
      661 attcaattta tagcagcaca accaaacgcc aaaaaaataa taaccaagag ctataaataa
      721 agattaaaaa tattttcagt gaatatgaaa ttattgctat ttctattaca aagatgtcct
      781 ggtctctctt tcattctctt cttgtcaaca tcgcgacaac tttcgtaaaa cactcctgcc
      841 ccgcttcagg tatactgtgc accttaattc cacaaacatc aggcagacca tgacagactt
      901 aatccaacgc cctcgtcgcc tgcgcaaatc tcctgcgctg cgcgctatgt ttgaagagac
      961 aacacttagc cttaacgacc tggtgttgcc gatctttgtt gaagaagaaa ttgacgacta
     1021 caaagccgtt gaagccatgc caggcgtgat gcgcattcca gagaaacatc tggcacgcga
     1081 aattgaacgc atcgccaacg ccggtatccg ttccgtaatg acttttggca tctctcacca
     1141 taccgatgaa accggcagcg acgcctggcg ggaagatgga ctggtggcgc gtatgtcgcg
     1201 catctgcaag cagactgtgc cagaaatgat cgtcatgtca gacacttgtt tctgtgaata
     1261 cacttctcac ggtcactgcg gtgtgctgtg cgagcatggc gtcgacaacg acgcgactct
     1321 ggaaaattta ggcaagcaag ccgtggttgc agctgctgca ggcgcagact tcatcgcccc
     1381 ttccgccgcg atggacggcc aggtacaggc gattcgtcag gcgctggacg ctgcaggctt
     1441 taaagatacg gcgattatgt cgtattcgac caagttcgcc tcttcctttt atggcccgtt
     1501 ccgtgaagct gccggaagcg cattaaaagg cgaccgcaaa agctatcaga tgaacccaat
     1561 gaaccgtcgc gaggcgattc gtgagtcact gctggatgaa gcccagggcg cagactgcct
     1621 gatggttaaa cctgctggag cgtacctcga catcgtgcgt gagctgcgtg aacgtactga
     1681 attgccgatt ggcgcgtatc aggtgagcgg tgagtacgcg atgattaagt tcgccgcgct
     1741 ggcgggtgct atagatgaag agaaagtcgt gctcgaaagc ttaggttcaa ttaagcgtgc
     1801 gggtgcggat ctgattttca gctactttgc gctggatttg gctgagaaga agattctgcg
     1861 ttaatttttt tcctctcaga aagggggagt taacctgttg aaagtacggt gaacgccccc
     1921 tcaccctaac cctctcccca agggggcgag ggaaccgtcc acactcgtat caccccgccc
     1981 gataaaacgg tttatcccca aggatcgtcg cccgatgcat aatccgccgc tgtggcaggt
     2041 aatcggcatt ggcatagtgc tgagtcacgc ggttatccca aatcgcaata tcattcggtt
     2101 gccagcgcca acgcacctga aactccggtt tagtgatatg ggcgaataaa aagcctaaca
     2161 aggcttcgct ctctttctcg ctgacatcga caattcgcgt agtaaagcct tcattcacaa
     2221 acagcgcctg tttaccgctc accggatgcg ttcgcaccac cggatgtagc aacggaggat
     2281 ttttcgcgac cgcctcgcac cagcgttgat gttcctcctc ggttttgcgg tatttgtatt
     2341 ccgggaatga tttacggaaa tcatgctccg cacgcagcca gctcagcagc tggcggaagg
     2401 gaacagagag cgcctcatag gccgcaatac cgctggttca gagcatatca ccgccggtcg
     2461 aaggtaactc tttagctgcc agaatcgccc ctgcgggtgg cgtttcaata aatgtcacat
     2521 cggtatgcca gttgtcgtta tccggcggat tatcgttatg ggtatccagc acgatgatct
     2581 cgtcaacccc ttcggcatga gggtaaacag ggtgaatatg cagttcgcca aaacgctggg
     2641 ccagcgcgcg ttgctgctgc ggcgtaatag cttgatcgcg cagaaatacc acctgatggc
     2701 gcagcaccgc atggtaaagc tgttcaaact gattatcgct taacgggcgc gtcaggtcgg
     2761 cacccgaaat ttgtgcgccg atatgcggcc ccagcggggt aatgctcaga cgttcaccca
     2821 ttgtacttct ccatgccagg gcgtcagacg gcgctgtaac gcgcgcagac ccagttctaa
     2881 aagaaaggcg ataatcgcaa tcaccgcgat ccccgccagt accacgtcag tcgcgagaaa
     2941 ttcacccgct gactgaacca taaatcctaa accgcgtgtc acggcaatca gctccgccgc
     3001 taccagcgta gaccagccca cacccagacc aatgcgtaat ccggtgagga tttccggcag
     3061 cgcgccgggc aaaatgacaa accacagcac ctgcgcacgg ctggcaccca gcgactgggc
     3121 ggcacgaatg cgaacctgct gcgcgctttt cacccctgcc agcgccgaca tcgccaccgg
     3181 cgcaaaaatc gctaaataga tcagtaagat cttcgaggtt tcaccaatac caaaccagat
     3241 caccatcagc ggcaaataag ccagcggtgg caccggacga taaagttcga ttaccggatc
     3301 cagaatgccg cgcaccgtcg ggcttagccc catggcgatc ccgaccggaa taccgatcac
     3361 caccgccgcg agtagcgcct gcacaatgcg cgtcaggctg gcagccagat gctgccacag
     3421 cgtggcgtcc ataaatccct gcggtccggc aatggtgagc agttttgcca gtacttgttg
     3481 cggcggcggc aaaaacagcg ggctaatcag ttgcagcgcc gccaccgtcc accatacagt
     3541 gagtaaaacc gctaacgtgc caatgcttaa ggtcacctga cgcgagagcg gccagcgcca
     3601 tttcagccgc cgcgaatgca gtttttcatt aatgagcaca ctcatgagaa cgcctcccgt
     3661 tgctcaaata cgcggcttaa aacatattcg cgcatggcga tgaattgtgg atcggacttg
     3721 atgctgcggc tcgactctcc cgcaacaaag cggcgagcaa agttgagcgg cagccgctcc
     3781 agcacacggc cagggccgga tgaaagcaga accagttcag tcgccataaa caccgcttct
     3841 tctatatcgt gggtaatcaa cagcacctgc ttgcccgtct cctgccagag tttcagcagc
     3901 agagtttgca tctggtcgcg ggtgaaggcg tccagcgcac caaacggttc atcgagtaat
     3961 aacagctggg gattcgccgc cagcgcgcgg gcaatcccca cccgctgacg ttgaccatcg
     4021 gaaagctgcc agatatagcg tttttctgcg ccttccagcc ccactttttt cagcatctgg
     4081 tgcgcgattt ccagtcgctg cattttctct atacctgcca attgcaggcc gaacgccacg
     4141 ttgtcctgta cattgcgcca cggtagcagc ccttcattct gaaaaactac gccacgctct
     4201 gctcccggtc cctcaatacg cttacccgcc agttgaatgc tgccatgctg ataaggcaca
     4261 aaaccggcaa tcagattcag cagggtggtt ttaccgcagc cggacggccc cagcaccacc
     4321 agtagctcgc cgctttccag cgtcaggttg atatcttcca gtgccggttt gccgccataa
     4381 tcggcataga gatgagagat ttgcagcatc tgcgcctcct tttattgcac gaagcgcgag
     4441 gtaacgtact ggctgtaatc attcgctaca gccgggacct tgccctgctc tttcaaaaac
     4501 tgcgcggtgt cgatgattgc tttgttcacc ggtccggtca gttctgccgt ttgttgctgc
     4561 ggcgtcagat aggtatttcc cttcaccagc cccggaacgt caccttcagg cacgccgctt
     4621 aaacgcgcca gtttgctgat gttttccggc tgtttcagcc acgcgtcagg gttagcaatg
     4681 tacggttgct gtgcatcgat ggcgcttttg gcgaacgctt tcacgacctc aggatgtttc
     4741 tcggcaaaat ctttgcgcac cacccagacg tccagcgttg gcgcacccca ctgcccgacc
     4801 tgttcagaat cggtcagcac cttgtcgtct ttttccaggg cgttaaccgc cggtgcccag
     4861 acataagcac catcaatatc accccgctgc catgcggcga taatcgcggg cggctgcagg
     4921 ttcacaatct ccactagccc tggtttaatc ccccagtgtt tcagcgccgc cagcaggctg
     4981 tagtgggtgg tggagataaa cggtacggca atgcgtttac caatcaaatc ttcgggtttg
     5041 ctgatagttt tctttaccac cagcgcttcg gagttaccca gttttgacgc cagcaagaag
     5101 acttcaatcg gcacctgttg gctggctgca accgctaatg ggctggatcc gaggttgccg
     5161 atttgcacgt cgccagaagc cagcgcccgc acgatgctgg ctccgctgtc aaacttacgc
     5221 cagtccacgc tcgccccgct ctctttagca aaggtgttgt cggcctgagc cactttcgcc
     5281 ggttcggctg aggtttgata cgccacggtg acgttcaccg cctgtgcctg aaaagcgatg
     5341 aatgccagtg cggcaagaag tgtgttatat gactaggtaa tttattcaag ataaccggga
     5401 atgggtaaaa aaagacggga tttgatggga ttcaataagt tatgcgccga ttgcattcaa
     5461 gataaaacga gttaaaaatg ctcaatgctt cgatatcgat aaaacattca agataggcgc
     5521 gccgtgtatt ttaattcttt taagactctt caaaaatgtc atcgtagcct ttaaaaacga
     5581 attttaaagg caccctaaag gcaggtcaat gaagttaacc cgccttcgcg tttacactct
     5641 ctgagagaat actagcttca atctcttcca aaatctctct gacttgctcg tcgggcatat
     5701 caacgagcat caacgctgcc aggcgtttac ggccctctag ttgcagtagc tcttgagttt
     5761 ccttatctaa caagcgtagc acagtctcaa cacctttcct tgcgagcatg tttgtaaggg
     5821 cttctcgttc ctcactggta agaaacgaca acatttctgt tagctgatcc ccttggctta
     5881 aatcactttg tgttttttgt gtttttggtg gattcccgtt accggttttt agccattcaa
     5941 gactaacacc ttcactttct gcaatgcgta ccaatatgct ttctcgaggt tctggtgtat
     6001 ttcccttttt aaaatagctt tgtaacgtat tgatatttat gccacaggct ctggcggcag
     6061 cacttcttga tggataccgc ctaataagat aagccatgcg ctccacgata ggttcttttg
     6121 cctgctccat aaaagtacct ctaggtattt ttctgtataa aggttctttt ccatctatct
     6181 aattgaatgg aaaggtaatt ttaaaaatcc aaataaaata caaaaaaata ccttgtattg
     6241 gtgctttttg tgatctgcga ttttatttac tcggatatcc acgcgaatat ccgcgcgggt
     6301 aattttgtag ggtaaaggaa agatgagaca aagagaagat agtggccgcg attggccttc
     6361 gctgatgatt cagcaagcac tcaaagataa tgggtggtct tcctttcggg agctttcact
     6421 ccatgccggt tatgctcagg attcactgaa agcggtagtt cgtacgccaa acaaaaaata
     6481 cgaacgtgtt atttctgacg ctataggtgt accgccagaa atcatctggc ccagccggta
     6541 tcgtaacgat ggatttatca ggaaggcgtc gtgatatgca gtcgtacgtt accgtaaatg
     6601 accttctcgg tgttcctgga atgccagcca ccgcgaaagg catccgacag gcgttacagc
     6661 gtttttctcg tgctcttggt gacgttagtc gccgtcgagc aggtacaaaa gccattgagt
     6721 accatatcga ttgcctgcct gaggtaaccc aaaaggcact gcgtgaacgc tatgtggaac
     6781 aactggtggc tacagagaac aacgtctccg aagtgaagac cgttaccagg acggcgcgta
     6841 accctgatgc tgttcaggca gttgaagcat atcggggttc tccgcaacta atggaagaac
     6901 gcctgaatgc cctgactgaa aatcagcgcc gggtgtctga agcaagagcg gcgctggcag
     6961 tggaagtgct gaagctggaa agcgccggta acctcgggcg actgaaagcc attaactttc
     7021 tggttgaaaa agcccgtaaa ggtgagttgc cggaacgact gcaacaggct gcgattaacg
     7081 ctaatgcaaa acgtggcact aatcgcacca tcagccgtga tccgctttac cagtgggtac
     7141 tgaaatacaa ccggtcgcag aacgccgcag aacgactgtt gttactggca ccaggaaaac
     7201 gcgacgaaat aaaaccggaa gaaattagct ggctgcctga atttctggcg cagtatcgtc
     7261 aggctaacgg cagacctatg tcggaagcct acgaggattt tgtcacggaa tggcaacgtc
     7321 gctatgcgga cgaaccgtac atgctggagg tgatgccttc ctatgatgtt gtccggtatg
     7381 ccatgaaaaa attgccggaa gtggtgaaac aaaaaggacg cgttaccggc agtgaatacc
     7441 gccagcttga gggattcacc cgccgcgact ggacagctat gccggtgaat tatgtctgga
     7501 ttggtgacgg ccacggtatg aagctgaaat gtgcgcatcc gatccacggg cgtccatttt
     7561 caccggaagt cacgtttgtt atcgacggtg gaacccggtt tgttgttggc tggagtcttg
     7621 atcttgcgga gaacgtgttt gctgttgccg gggcgataca gcacggaatt cgcaatcacg
     7681 gaaaaccctt cctgtattac tcggataatg gttccggtga aacggcagat atgctggata
     7741 aagagatcgt cgggattctg ccccgtctgg gtattaacca ccccaccggt attgccggaa
     7801 atccgcaggg acgcggcatc attgaacgtc tgaaccggac gctgccgatg cgtatagcca
     7861 gacggtatcg cacctatttt ggcaaggggg cagaccggga aacactgcgt gtactcaatc
     7921 gcgatctgcg ttcagcattt aatgccctgc aacaggacaa accgctgaat gcccgccaga
     7981 aatctgcaat gcgcgagctg ccgtcctggt cagaactgat tgaggctatt cgtgaaggag
     8041 tggaatggta caacaaccgc ccgcattctg aactgccgat gaaaccaaac gggaagcatt
     8101 acagcccggc tgagttcaga aaaaaacgcc tggcggaaga aaacaccgaa attgaatggc
     8161 tgagtgatct cgaactgcgc gacatgttct gcccgatggt ggagcgtccg gtaagacgct
     8221 gtgaaatcaa atggcttggg aatatttatt acgcgccgga gctgcgtgat gaacatggtc
     8281 gcaaagtgct tatcagctat gacattcatg atgcagagcg aattacagtg cgccgtaagg
     8341 atggcagttt tatttgtgag gcgatatgga acggcaataa acgcgccgcc ttcccggtca
     8401 gcgccgacta ttacaaacaa cagcagcgta ttaaaggaat gcacaaacgc gcagaagaaa
     8461 aaatccgtga tgccgtggat gaaggtattc aggttattga acaaaaaacg acagaaccct
     8521 ggctggataa tgtttatcga cccgttggca atgtgatgac cgttcagcaa caggaatatg
     8581 aagaggagca cgacgaagaa tccgaacgca atttcaggcg gggattacag aaactcattg
     8641 ccatgcagga cgatgaccca ctggcctgaa taaaaaaaca gtccgcagca acggactgtt
     8701 aaacaggtaa ataaagctga tttgaaataa ccgaggaaac aatatgaccg atattaatga
     8761 t
//
LOCUS       DAJVQH010000143         8702 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJVQH010000143 DAJVQH010000000
VERSION     DAJVQH010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.143"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(14..754)
                     /gene="cysQ"
                     /locus_tag="OQM87_003564"
     CDS             complement(14..754)
                     /gene="cysQ"
                     /locus_tag="OQM87_003564"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCS1509549.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            944..2887
                     /gene="cpdB"
                     /locus_tag="OQM87_003565"
     CDS             944..2887
                     /gene="cpdB"
                     /locus_tag="OQM87_003565"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCS1509550.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3013..3393)
                     /locus_tag="OQM87_003566"
     CDS             complement(3013..3393)
                     /locus_tag="OQM87_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1509551.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3482..4342
                     /gene="qorB"
                     /locus_tag="OQM87_003567"
     CDS             3482..4342
                     /gene="qorB"
                     /locus_tag="OQM87_003567"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1509552.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            4451..5416
                     /locus_tag="OQM87_003568"
     CDS             4451..5416
                     /locus_tag="OQM87_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1509553.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5524..6186
                     /gene="ytfE"
                     /locus_tag="OQM87_003569"
     CDS             5524..6186
                     /gene="ytfE"
                     /locus_tag="OQM87_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCS1509554.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6231..7643)
                     /gene="cycA"
                     /locus_tag="OQM87_003570"
                     /gene_synonym="dagA"
     CDS             complement(6231..7643)
                     /gene="cycA"
                     /locus_tag="OQM87_003570"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCS1509555.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(7951..8571)
                     /gene="fklB"
                     /locus_tag="OQM87_003571"
     CDS             complement(7951..8571)
                     /gene="fklB"
                     /locus_tag="OQM87_003571"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1509556.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALIAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         2206 a         2295 c         2204 g         1997 t
ORIGIN      
        1 ctgccatctg aatttagtaa atggacactc tgaaccccgg attcaggaac gactcgcgcg
       61 gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg cgctccggca gctgcagcca
      121 cagcatgtcc agcggcggtg tcccaaatat tcgttggtcc gaagcgcggg tacagctgcg
      181 cctgtccttc cgccaccagg cagaatttca gcgaagagcc gatggacgtg gtctgatgtt
      241 cgccaagctg ttgcagatac tctttcagct ccgcatctgc atgggaacgg ctgataacca
      301 ccagcggcgg gcgcgcatcg cggacctgaa tcagcttgcg cacaccgcac tcttctttcc
      361 aggccttgcc ttctgccgcg ctgtacatta cgttcattac cggcgcatac accacaccta
      421 aaatcggttt gccatggtca atgagcgcaa tgttaacggt gaattcgcca ttacgtttaa
      481 taaactcttt agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga
      541 cttcccaacc gggaggatct tcttcagaaa ggaccggaat atccggtgcc agcgtacgta
      601 aaccgtccat gataacggtg tgagcggcaa tatccgctgc cgttaccgga gaattgtccg
      661 ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg
      721 cattccgtgc aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt
      781 gttaacttat tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc
      841 atcattttca aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa ggcgtttatt
      901 ctcccttttc tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc
      961 aacgctcctg gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat
     1021 catggaaacc actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc
     1081 cacggaaaaa ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt
     1141 gaaaaacagc gtactggtcg ataacggcga tttgattcag gggagtccgc tggccgatta
     1201 catgtcggcg aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac
     1261 gctggattat acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct
     1321 gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag
     1381 aaccaaacag ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga
     1441 cggaaaaaaa cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg
     1501 ctgggataaa gctaatttat ccggaaaagt gacggtgaat gatattaccg aaaccgtgcg
     1561 caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc
     1621 cgggctgtct gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga
     1681 aattccgggc gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga
     1741 ttttgctgat atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt
     1801 aatgccgggc atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag
     1861 cggtaaatgg caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa
     1921 taaaaaatcc ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga
     1981 tgccacacgc cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta
     2041 tctggcgctg gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta
     2101 tgtcgaacat tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc
     2161 cgcaccgttt aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa
     2221 aggccagttg accttccgta atgccgccga tctttatctc taccccaata cgctgattgt
     2281 agtgaaagcc agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa
     2341 ccagattgct cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac
     2401 ttataacttt gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg
     2461 ctatgacggc gagtgccaga tgattaatgc caatgcagag cggattaaga acctgacctt
     2521 taatggcaaa ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc
     2581 ttacggcggc aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga
     2641 gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca
     2701 tccggcggca gataacaact ggcgtttagc accgatagct gccgataaga aactggatat
     2761 ccgtttcgaa acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta
     2821 tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag
     2881 taagtaaaac acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat
     2941 gcgatgctgc cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag
     3001 cgcatccggc agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa
     3061 ctcaatccag tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag
     3121 gctatattcc acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg
     3181 ttctaacgcc tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg
     3241 caggtcgcta aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg
     3301 gctggtgacg tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc
     3361 tttcagttgt tgtgacaggc taacctgact catttcatac ttaccttttt gcacgtactt
     3421 actaaaagta agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc
     3481 catgatcgct attactggtg ccactggcca acttggtcac tatattattg aatccttgat
     3541 gaaaacggtt cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc
     3601 cctggcagca caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac
     3661 atctgcactt cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg
     3721 tgccccgcag catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc
     3781 ttataccagc ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga
     3841 gacggagaaa atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta
     3901 cagcgaaaac tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc
     3961 ggcgggcgat ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcact
     4021 cgtgattagc gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg
     4081 gacgttgaca cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca
     4141 aaatctgagc gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact
     4201 ggcggatatg ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag
     4261 caaaacgctt agcaaattga ttggccgccc aacgacaacg ttagccgaaa gcgtaagcca
     4321 tctttttaat gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa
     4381 tgacgggatg ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg
     4441 gatgccagta atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct
     4501 tatttttgtc gggccgttga tcgtgccgga atacccggcg atgttacagt cgatggggcg
     4561 ttatctggcg ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg
     4621 tcagttggcg cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat
     4681 ttattacttc tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat
     4741 tatcggcacc ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga
     4801 cggcaaactc gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc
     4861 gagtgtgaat attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac
     4921 ctctggcatc gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa
     4981 cgcccgctgg ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggcgcaggc
     5041 gctggtcacg ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat
     5101 acaaacgccg gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat
     5161 ggttgcgata gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca
     5221 gcgattaccg acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct
     5281 gtacaccttt ttactccgcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct
     5341 gttagtgatt ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc
     5401 tgtctcagaa agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta
     5461 tagcttttgc cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca
     5521 gctatggctt atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg
     5581 gctctgtttc gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc
     5641 gcggcggcac gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct
     5701 gaacaaccga ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc
     5761 atcgtgcgct accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact
     5821 aaagtcgagc gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg
     5881 accatgctgc atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg
     5941 atgatcaaac aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag
     6001 cacgatgaag cgggcgaact gctagaagtg atcaaacaca ctaccaataa cgtcacaccg
     6061 ccgccagaag cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat
     6121 gacctgatgg atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt
     6181 gagtgatcta aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc
     6241 agttcagcag cccgcttctt accaataaac agccagccca accccagcgc gataaaccac
     6301 agcggggtaa ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg
     6361 aacacaaaga acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc
     6421 gacttttcat gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc
     6481 gtccagacga acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc
     6541 acgctaggat tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc
     6601 aaccctttcg cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct
     6661 tcctgcgcca gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac
     6721 gccgcagagg tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac
     6781 acaaacagct caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac
     6841 ataatcacaa tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc
     6901 gcgcgtggca gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca
     6961 atccccacga aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc
     7021 gggaaccagc cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac
     7081 tgaaagtgca tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt
     7141 ttgatcatcg caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc
     7201 gtcagcagca gcactatcac cgccagcgag gcgacccagt cggagaaatc ggggaaccag
     7261 aactgagcat aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac
     7321 cagtaagtcc agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca
     7381 ctaaaagatt tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa
     7441 aagagcataa aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc
     7501 gttttgccgg accccataaa caacccagta ccaatggcac cgccaatggc aataagctga
     7561 atatgtcggt ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca
     7621 acgactttta cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct
     7681 ctacggcctt tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg
     7741 taacaatacg cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg
     7801 agtgtcatat cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt
     7861 tgcaacacaa aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc
     7921 ctctggttcg aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa
     7981 caccagggtg ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc
     8041 ctgcgggata gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca
     8101 gccagggatc acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc
     8161 aaacacggtg ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc
     8221 cggaattgcg ccttcacctt ggttgatcac gcggaattgc aggccagatt cggtgctatt
     8281 cacaccttct tttttggcgt tttcttccag gtatttcaca ccttcagcag ccatcgcctg
     8341 gaaacgctga cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac
     8401 cacatcaacc ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaatcag
     8461 tgcttctggc agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc
     8521 aatgccgtag cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc
     8581 tttcttaggg tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt
     8641 gtcgcaggaa tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat
     8701 at
//
LOCUS       DAJVQH010000144         8676 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJVQH010000144 DAJVQH010000000
VERSION     DAJVQH010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.144"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(135..2678)
                     /gene="mdoH"
                     /locus_tag="OQM87_003572"
     CDS             complement(135..2678)
                     /gene="mdoH"
                     /locus_tag="OQM87_003572"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCS1509557.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2671..4206)
                     /gene="mdoG"
                     /locus_tag="OQM87_003573"
     CDS             complement(2671..4206)
                     /gene="mdoG"
                     /locus_tag="OQM87_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCS1509558.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4600..5757
                     /gene="mdoC"
                     /locus_tag="OQM87_003574"
     CDS             4600..5757
                     /gene="mdoC"
                     /locus_tag="OQM87_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCS1509559.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5765..7246)
                     /gene="clsC"
                     /locus_tag="OQM87_003575"
                     /pseudo
     CDS             complement(5765..7246)
                     /gene="clsC"
                     /locus_tag="OQM87_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
     gene            complement(7188..7721)
                     /gene="ymdB"
                     /locus_tag="OQM87_003576"
     CDS             complement(7188..7721)
                     /gene="ymdB"
                     /locus_tag="OQM87_003576"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCS1509560.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7816..8127)
                     /locus_tag="OQM87_003577"
                     /pseudo
     CDS             complement(7816..8127)
                     /locus_tag="OQM87_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(8248..8580)
                     /gene="csgC"
                     /locus_tag="OQM87_003578"
     CDS             complement(8248..8580)
                     /gene="csgC"
                     /locus_tag="OQM87_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCS1509561.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
BASE COUNT         2175 a         2244 c         2055 g         2202 t
ORIGIN      
        1 ggtattttta ttggctcgtt gtttttgccg gatgtggcgt gaacgcctta tccgacctac
       61 aaaatcttgc aaaatcaata aattgcagga acgatgtagg cctgataagc gtagcgcatc
      121 aggcaactac gtttttattg cgaagccgca tccggtttac gcaatgccag tggattgagc
      181 tttatccctt cgtaataact cacccatgaa gaatatcttt caggggaatt ccagacgcgg
      241 aaatgcagac gggccatcgt caccggatcg cttagcagca ccaggcgacg atcgcgattc
      301 agcttctctg gcgtctcgtt aagcgcctgt tcaacgtggc ggtcacgggc gatttccagc
      361 accttactgg cgcggtgacg cgctgtcgcc attgcggttg ccagagcgtt aaatgacggg
      421 ttaaacactg cgtgcataaa gccatcgtca agggagcgtt ggcgattcat ctcaaggaac
      481 cgatcggtat caaccagcac ctgcggcggt gaatactctt ccgggatcag gaacagtttc
      541 cagcgtttgg tgcgcagacc aacggtggca cggctggaaa tcaccgaaac aaacggtgac
      601 aggatcaacg agaagacaat cggtgccagc cagaacagga aacgcagatc cagccacgcc
      661 atcccaacag cccacactaa ccccaacagc agctgtgagc cgtggcgttt gaacgcttcg
      721 ccccatgaag tggagtcatc atcacgctgc ggtgaattcc acaccacttc ccagccaagg
      781 aacgcgctga caacgaagac cgtatggaac agcatgcgta ccggagccag cagcacggaa
      841 aacagcactt ccagcagcaa cgataatgta acgcgccaga agccgccgta ttctttcgtt
      901 cctttgcacc agataagcaa aatgctcaat aacttcggca ggaacaacag caccatggtc
      961 gaagcaaaaa gtgcaatcgc cagctcagga cgccactgcg gccacaccgg gaacaactgc
     1021 cgtggttgca ggaagtattg cggttcggtc aacgcatgca ctacctgcaa tgcagtagag
     1081 agcgcgagga acataaacca cagcggagcg gagagataag acatcacgcc cgtcaggaac
     1141 accgcacggt gaaccgggtg cataccctta accaggaaca gacggaagtt catcaggtta
     1201 ccgtggcacc agcggcggtc acgttttagc tcatcaagca agttaggcgg caattcttca
     1261 taagaacccg ggagatcgta agcaatccag accccccaac ctgcacggcg catcaacgcc
     1321 gcttctacga agtcatgtga caggattgaa ccggcaaagg aaccttcgcc cggcagcgga
     1381 gccagtgcgc agtgctcgat aaatggtttc acgcggataa tcgcgttatg tccccagtag
     1441 tgcgactcgc caagttgcca gaagtgcaaa ccggctgtaa acagtggccc atacacgcgg
     1501 gtcgcgaact gctgacagcg cgcatacagc gtatccatac cggacgcttt cggcgacgac
     1561 tgaatgatcc cggcgttcgg gttggcttcc atcaagcgca ccaggccgca taaacaatca
     1621 ccggtcatta ccgagtcagc atccagcacc accatgtagc tgtactggct gccccagcga
     1681 cggcagaagt catcgatatt accgctttta cgcttcacgc ggcgacggcg gcggcgatag
     1741 aaaatctgac cttcgccacc gacttcagcg ataagctcca tccaggcttt ttgctctgcg
     1801 acgcagatat ccgggttata actgtcactc agaatgtaga catcaaagtg tttggcattc
     1861 ccggtggctt ttactgattc ccacgttgca cgcaagccag caaaaacacg gttcacgtct
     1921 tcgttacaga taggcatgat caacgccgtg cgatgctccg ggtttaatgg ttcatcgcca
     1981 actgttgacg cagatatact gtatttatcg cgaccaataa gcagttgcag gaagcccatc
     2041 aacgccgtcc agaatccggc ggacacccaa cagaacagta ccgcaaaaag gatcaggata
     2101 ccggtttgca gcatataagg cagaagctgc ataaaggaaa cccacaaatc ctgaccaacc
     2161 atatccatag gattaatcag cgcccaaccc tgataaggaa gaatggtctt catataccag
     2221 gtcgcgacaa cagtttgcgc gagcgtcagg atcaacagaa tgtaacggcg gatggtacca
     2281 acggtacgcc acttttgctc gctctcctgc tcttctttgg tcaaacgagc cagatagcgc
     2341 ggcgtgacat cgcgtccacg caggcgatcc cagaaacggc ctaccgggtt ggtacgccac
     2401 gggtcgggaa acatcgagga gcgttttgct tctggcatcg ccttaagctg atcgcgccct
     2461 tcgtcgtctt taattaactg tccatcagca agtgaatctg gccaggcttg ttccagacgc
     2521 gcctttaccg agccttgcgg ggaatcatcc tcccgcgccc aggtgcggtg ttcggcatcc
     2581 agcgcctgat gaacggcgcg gatatcagtc ttcggcaatg ctgctttctc gcttgcggcg
     2641 atgggcattg cgtcaatgta ctcagttgtc ttattcattg gcaggtaact ggtagctcca
     2701 ggtttcactc aacgtctgat cggcattcac cagcgcagca cgcatttcag tggttttctt
     2761 ggcatctttc actttcacac gcatcaccag acgccagcct ttggtaaccg ggttatagcg
     2821 caccgtgctt tcaactatct caccattatc accaatgctg gtttgcgctg tgaccggggt
     2881 atcctctggc agttttttca tctctgcgcc ggtaaaatcg accacaaagg cgatagtacc
     2941 gtcaggctgg cgaatcaggt tcgactgctt cacatccccc gttgaacgac gcgtttgttg
     3001 cacccatgcg ttatctggcg catgcagttt gtcttcatca cggctgaagg tgatggtgta
     3061 tttaaagttc atctctttac ccggctccgg cagctgatcc ggcgtccagt aagcgacgat
     3121 gttatcgttg gtttcatcgt tggttggaat ttccaccagc tcaacgctgc ctttgcccca
     3181 ctcccctttc ggagtcaccc atgcgcttgg acggagatcg taacgatcat cgagatcttc
     3241 aaagcgggag aaatcacgac ctcgctgcaa cagaccaaag cctcgcgggt tttccatgga
     3301 gaagctgctg accgctaaat gtttcgggtt attcaacgga cgccagatcc attcgccatt
     3361 accagcatgg atagacagac cgttagagtc gtgcaactcc ggacgatagt tatttgcagg
     3421 cgacggttgg ttcggcccaa acaggaacat actggttaac ggtgcaaccc ccagtttgcc
     3481 gactttatcg cgcagataga ttttcgactg cacatccaca accgtgtcac gccctggcat
     3541 aaccacgaat ttgtaagcac ctgtcgcacg cggcgagtca agcaatgcat aaatggttaa
     3601 acgtttatca gtcggttttg gacgctcgat ccagaactct ttgaagcgtg gaaattcttc
     3661 acccgatggc aaagcggtat caattgctag gccgcgggca gaaaggccat aaacctgacc
     3721 tgcaccaatc acgcggaaat agctggcccc gagcatgctg acgatttcat cgtttttatc
     3781 tttgctgttg atcgggtaaa gcactttaaa accggcaaaa ccaaggtctt ttaccgtgtc
     3841 tttgtcatgc tgaacatcgc cgaaagtgaa ataatccggg ctgtatttga ttcgtttgac
     3901 tgcggtggca gtcacttcat ttattttgac cggggtatcg aagtacatac cctgatggta
     3961 gaactcgagt ttgaatgggg tcttcagatt gttccagtac gctttgtcat gattaaactg
     4021 gatctgctga tagtccgcgt atttcatgtc gcggaaaacg gagggcaagt tgcttttagg
     4081 cgcctcatag cctttcccgg ctaaggattg agcttgcttt gcgacatcat caatactgaa
     4141 agcccagctt gaagatgtat acagggttaa cattactgca gcactcaacc aacgcatttt
     4201 catcatttgt agtttatgtt tcataattag taagcacttc cccctttgtg tgcttatatc
     4261 gatccgatct attttaatgg ataaccagcc attccgacaa ctgaatcccg gtatcgttca
     4321 gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc gtgcttctcc
     4381 taaagacaga agcatcgact tcgtgtaggg tttgccagca aatttattta tcaaccgtct
     4441 tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa accttgaagc
     4501 ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg cataacccga
     4561 cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt acccgcgcaa
     4621 cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat cccttttcat
     4681 atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc gtcattgtgg
     4741 ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt cgttatatcc
     4801 ggatactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg gaaagtacgt
     4861 gtcgaacgtg taggtatccc gatgttaaca gccatccccc tactgacatt accgcaattt
     4921 attatgctgc aatatgtcaa agggaaagcg gaaagttggc ctgggctatc attgtatgac
     4981 aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact ggtgttagta
     5041 gtcatgacga cgctgtgtgt atggatattt aagcgcatca gaaataattt agaaaattct
     5101 gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt atgcctcggc
     5161 atcggctatg cggtaataag aagaacgatt tttattgtgt atccacccat tctgagtaat
     5221 ggcatgttca attttattgt catgcaaacg ctattttatt taccgttctt tatcctcggc
     5281 gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc tcgtggctgt
     5341 accctcgctg cagcattggc gtttgtcgct tatttactca accagcgcta tggcagtggc
     5401 gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggcct gtggatggtg
     5461 aatgtggtct tctcctttgg ccaccgttta cttaacttcc agtcagcgcg ggtgacttat
     5521 tttgttaacg catcgctgtt tatctatctg gttcaccacc cgttaacgct gtttttcggc
     5581 gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg cctgatattc
     5641 gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt actgaagttt
     5701 ttgttctctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc ccgttaagcc
     5761 acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata acccgcttcc
     5821 agaaactggt ggcgggttct tttttgagaa taacctcttt ctttgcatga cgatcaaccc
     5881 agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcgtac tggctctgaa
     5941 taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag cccatttcag
     6001 tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc gttttaccat
     6061 cgatgctaaa agttttagca tgcaggctgg caccggaatt accggttatg ccgcgatcgt
     6121 gtaacgtact gctttgttca cgcgtcggct tgagttcata taattccacg ccatagcgga
     6181 gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg ttagcggcaa
     6241 gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag agttgcgcca
     6301 cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca ctgggtgagc
     6361 ccatgatatc gaacaggcgc tgcggtaaca gtgaatgccg ttttgccttg ccctcccctt
     6421 tcgccggatc atcacttaat aaacgtgtct tcgcccagat aagcggcaac gttccatcaa
     6481 ccagatgatt tataaatgga ctggattcca ttttgcgtaa ataacgatgc gtcatggcat
     6541 cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc gggacatcca
     6601 gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa tcatcggcaa
     6661 cgtcctctac cacggggcct attgccatga catctaaatc cgaaaaaagt cgctcttccc
     6721 ctgcactaaa ataggcatca ccaatattta gtcctcccac tagggtaacc acgccatcga
     6781 cagtgaaact tttattgtgc atccggcgat tgagacggga aaagtcggtg atatatccaa
     6841 gcggacgtag caagcgaaac gagaaaggat taaaaagccg gacttcaatg cgtggatgac
     6901 tgtcaagcaa gcgtaaaatg tcgtcaagtc cgggcgtatt gttgtcgtcc agcaacaaac
     6961 ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac cgccccgaca
     7021 tatcgtccta ccagatgtaa tactgaacat ccagcgtatg ctctgccatt tcggccagac
     7081 gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca cactgaccgg
     7141 gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca ttcatctcct
     7201 tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca gacaaagtat
     7261 acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac tgcgatttca
     7321 gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa agccactgac
     7381 gtatagctgt ttgccgccac cagtcgtagg ctattcagat aggcatcctg caaaagctgg
     7441 tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac ggctttagcg
     7501 ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc ctgctgttgc
     7561 ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat ggccccatcg
     7621 acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc aacggccagt
     7681 ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc ttacaccttc
     7741 ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc tcagcaacgt
     7801 gagttgttca gaaacttagt tatattcgat gttcattaca gccagctttt tctgtttatc
     7861 aagatactgc acttttactg aggctatttg cgcgtttttg acattgccgc tcattaacgc
     7921 tttgctggag taggttttgc ccggaataga accattctat gggcagttca tttctacggg
     7981 tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa aatggatcac
     8041 gcccccttgc gcggtgcttc ctgcaatggc aataaaagga aaaaacaaac tgccgagcat
     8101 aagagagtct aaaaaaggtc tgaacattta ttctatttcc tcaatgatta gtcatccttg
     8161 agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt ctctccagat
     8221 tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg tccattgttg
     8281 tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc gatcttccgg
     8341 ggaaatattt aaactcaact tcgtcaaagc aatgggttga ttagcaggca atgagagggt
     8401 cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca atatttgtac
     8461 tctgcacaga caagattgag taagagtaac ttcaggaata atggtgtaca tatccccttg
     8521 ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata acgcattcat
     8581 attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc tgtaatacaa atgatgtatt
     8641 aatactgatg agcggtcgcg ttgttaccaa agccaa
//
LOCUS       DAJVQH010000145         8501 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJVQH010000145 DAJVQH010000000
VERSION     DAJVQH010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.145"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..962)
                     /gene="trkA"
                     /locus_tag="OQM87_003579"
     CDS             complement(<1..962)
                     /gene="trkA"
                     /locus_tag="OQM87_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCS1509562.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKR"
     gene            complement(984..2273)
                     /gene="rsmB"
                     /locus_tag="OQM87_003580"
     CDS             complement(984..2273)
                     /gene="rsmB"
                     /locus_tag="OQM87_003580"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCS1509563.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2319..3266)
                     /gene="fmt"
                     /locus_tag="OQM87_003581"
     CDS             complement(2319..3266)
                     /gene="fmt"
                     /locus_tag="OQM87_003581"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCS1509564.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3281..3790)
                     /gene="def"
                     /locus_tag="OQM87_003582"
     CDS             complement(3281..3790)
                     /gene="def"
                     /locus_tag="OQM87_003582"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCS1509565.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            3938..5062
                     /gene="dprA"
                     /locus_tag="OQM87_003583"
     CDS             3938..5062
                     /gene="dprA"
                     /locus_tag="OQM87_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS1509566.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5034..5507
                     /locus_tag="OQM87_003584"
     CDS             5034..5507
                     /locus_tag="OQM87_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCS1509567.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            5536..6078
                     /locus_tag="OQM87_003585"
     CDS             5536..6078
                     /locus_tag="OQM87_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCS1509568.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6083..6655
                     /gene="tsaC"
                     /locus_tag="OQM87_003586"
     CDS             6083..6655
                     /gene="tsaC"
                     /locus_tag="OQM87_003586"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCS1509569.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            6660..7478
                     /gene="aroE"
                     /locus_tag="OQM87_003587"
     CDS             6660..7478
                     /gene="aroE"
                     /locus_tag="OQM87_003587"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCS1509570.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7475..7732
                     /locus_tag="OQM87_003588"
     CDS             7475..7732
                     /locus_tag="OQM87_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCS1509571.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(7708..8262)
                     /locus_tag="OQM87_003589"
     CDS             complement(7708..8262)
                     /locus_tag="OQM87_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCS1509572.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2112 a         2065 c         2142 g         2182 t
ORIGIN      
        1 atacgtttgg caagcatggc ggacatgata ttggcctcgt catcgttggt gacggcaata
       61 aacagatcaa cttgatcgat atgttcttcg gccagcagtt cttgatccga cgcatcacca
      121 aaaaagacga tcgtattctg caacttttcc gccagttcgg cagcgcgctg ctgattacgt
      181 tcgatgagtt taacgctgta atctttttcc agacgacgcg ccagccctgc accgatatta
      241 ccgccaccaa ccagcatgat ccgtttatac ggtttttcca gtcgctgtaa ttcactcatc
      301 accgcgcgga tatgctgtga agcggcaata aagaacactt catcaccagc ttcaacaatg
      361 gtcgaacctt gtggacgaat ggggcgatcg tggcggaaaa ttgctgccac acgagtatcg
      421 atatgtggca tatgttcgcg catggtcgaa agtgcattac caatcagcgg gccgccataa
      481 taggctttaa ctacagcgag gctgacttta ccctcagcga agttcaccac ctgcaatgcg
      541 cctggatact caatcagtcg gtaaatatta tcgataacca actgctctgg tgcgatcaga
      601 tgatcaatcg gtacagcatc tgaatgaaat agcttatcgg catcgcgtac gtagtctggt
      661 gagcggatgc gagcgatgcg attaggggtg ttgaaaagtg agtaggctac ctggcaggca
      721 accatattgg tttcatctga actggttaca gcaaccagca tatcggcgtc gtcggcacct
      781 gcctcccgca atacgcgtgg atgagagcca tgcccctgca cgactcgcag gtcaaattta
      841 tcctgcaagg tccgcagacg ctcaccgttg gtatcgacaa cagtaatatc gttgttctcg
      901 ccaaccaggt tttccgccag tgtgccgcca acctggccgg cacccagaat gataattttc
      961 atcagtcgcg acccgttatc tcatcacttt ttgattagct tagcgtaaaa gaagccgtcg
     1021 ccctcttcgg caccaggctg attttgttta cccggttgct ctggtgttcc tgtttcgcaa
     1081 agttcggcat cagcggtacg ttgcaaaaag gctttaatct gcaggctatt ctcttccggt
     1141 aacaccgaac aggtggcata gaccagggtt ccaccggatt ttaaatgcgg ccaaatggcg
     1201 tcgagaattt cagactgcaa ttgcgcgagt tccgggatat cgcgatcgcg acgtaaccat
     1261 ttaatatctg gatggcgacg aatcaccccc gttgctgaac aaggcgcatc taataaaatg
     1321 cgatcaaact gttgctcgcc acaccattgg gaagggtaac ggccatcacc ttgtttcacg
     1381 gttgccttca taccaaggcg ttttaaattg tcgtaaacgc gagagaggcg ctgttcgtca
     1441 atatcaaccg caacaacctg cgcttctggt gccacctcaa ggatatgcgt tgttttaccg
     1501 ccgggggcgg cacaaagatc caagatgtgt tcaccgtttt gtggcgcaag ccaggtcatg
     1561 caaccttgtg ctgatgcatc ctgaacggta acccatccgt cttcaaaacc aggtagcgca
     1621 tgaacaggtg caggtgtttc cagccgtaca gcatcagggt aatccgcatg cgggaaacct
     1681 ttcattcctg cttcatccag caatgcaagc cagctgtcgc gggaatgatg cgtacgatta
     1741 atacgcagcc acattggcgg acgctggtta ttggcttcgg cgatggattg ccactgctct
     1801 ggatacgctt tttgcagacg cttcagcaac caggaaggat gcagataacg tgcatcacgg
     1861 gtattaaact cggctaataa ctcttcttgc cgacgctgga actggcgtaa tacaccgtta
     1921 atcaaccctt taagttgcgg acgcttaatt gcgatagcgc cttcaaccgt ttcagccagc
     1981 gcagcatgag gtggaatgcg ggtataaagc agttgataca aaccaaccat aatcaggtaa
     2041 tgcacagtgc gctgtttgcc ggtcatcgga cgggccatta acttattaat cagccagtct
     2101 aactgcgaaa gcgtacgcag tacgccaaag cacaactctt gaagaagtgc tttgtcttta
     2161 tcggaaactt tttgctggag cggtggcaga atgttgctta atgattgccc ttgctcgacg
     2221 acttgttcaa cggcctgggc cgccatgcta cgtaaattac gttgtttttt cataagtata
     2281 aaaatgctcg gcaagaccgg gcttagaaga gtggactatc agaccagacg gttgcccgga
     2341 acaaaccatt cccgacgaga gttcaggagg tcttgcgcgc tcatcgcttt cttacccgca
     2401 ggttgtaacg agagcaggtt caggatgcca tcaccagtcg caacctgaat gccttgtttg
     2461 ttggcttcaa ggatcgttcc tggtgcagcg ttggttgccg tatcaatgac cgacgctttc
     2521 cagactttaa ccggctgtcc ttcaatttcc agccagctca ttggccatgg gttgaaagcg
     2581 cgaatgcagc gttcaagctg tgctgccgaa agtgaccagt caatacgcgc ttcttcttta
     2641 ctcaacttct cggcgtaagt gacaagagtt tcgtcctgaa cttctggttt cgccgtgccg
     2701 tctgccaatt gtttcaacgt ggtgataagc ccttgtgggc caagctctgc cagcttgtcg
     2761 tacagcgtac cactggtatc ttctgcagta atcgggcagg agagcttata gagcatgtca
     2821 ccggtgtcta aaccaacatc catttgcata atggttacac cagtttctgc atcacccgcc
     2881 catagtgagc gttggattgg tgcagcaccg cgccagcgtg gcagcagtga accatgaacg
     2941 ttgatacagc caagacgcgg catctccagc acagctttag gcagaattaa accataggcg
     3001 acgacgacca taacatcagc ctgcaaatcg gcgaccagtt gctggttttc ttgtggacgc
     3061 agggaaacag gttgaaaaac gggcagacct tcttcctcag ccagaacttt aatcgggctg
     3121 ggcatcagtt ttttaccgcg tcctgccggt cggtctggct gggtgaacac gccaacgacg
     3181 ttatgaccag aagacaacag cgcgtcgaga tgacgcgctg caaagtcagg tgtacccgca
     3241 aaaataatac gtagtgattc tgacacgtta gttcttatcc ttaagcccgg gctttcagac
     3301 gatccagttt ttcaactttc tgacgaattc gttgttgttt cagcggtgac agataatcca
     3361 taaacagttt gccaaccagg tgatccatct catgctgaat acagatagct aacagaccct
     3421 ctgcttccag ttcaaatggt ttaccgtcgc ggtcaagggc gcgaatttta actttctctg
     3481 cgcgcggcac taaagcacgt tgttcaggga tcgacaggca accttcttca atgcctgttt
     3541 cgccgctttt ttctaaaagc tccggattga ttaacaccag ccgttcgtca cggttttccg
     3601 aaacatcaat aacaatgata cgttgatgga tatcaacctg ggttgccgcc aggccaatac
     3661 cttcttctgc gtacatcgtc tcgaacatat catcgacgat acgctgaatt tctgcattca
     3721 cttcttctac cggtttagca actttgcgaa gccgctcgtc cggaatatgt aacacttgca
     3781 aaactgacat aaatctccag agatgtgttc aggagttaga aagattattt cttctattct
     3841 agacaaatcc ccctctgatt gacagcatca cttgattgac agcatcactg accaatcgca
     3901 aagattgcta aggcttgctt atgacaggga gataaggatg gtcgatacag aaatttggct
     3961 gcgtttaatg agcatcagca gcttgtacgg cgatgatatg gtccgtatcg ctcactggct
     4021 ggcaaaacag tcgcatattg atgcggttgt attgcagcaa acagggctta cattgcggca
     4081 ggcacaacgc tttctttcat ttccacgaaa gagtatcgaa agctcacttt gttggttgga
     4141 gcaacccaac catcatttaa tccctgcgga cagcgaattt tatcctcctc aacttcaggc
     4201 gacgacagat taccccggcg cactgtttgt tgaaggagaa ctgcacgcgc tgcattcatt
     4261 tcagcttgcc gtagtgggga gtcgggcgca ttcatggtat ggcgagcgat gggggcgatt
     4321 attttgcgaa actctggcga cgtgtggagt gacaattacg agtggactgg cgcgtggaat
     4381 cgatggtgtg gtgcataaag cggccttaca ggtaaatggc gtcagcattg ctgtattggg
     4441 gaatggactt aatatcattc atccgcgtcg ccatgcccga ctggctgcca gtctgtttga
     4501 acagggtggc gctctcgtct cggaatttcc cctcgatgtt ccaccccttg cttacaattt
     4561 cccacgaaga aatcgcatta tcagtggtct aagtaaaggt gtactggtgg tggaagcggc
     4621 tttgcgcagt ggttcgctgg tgacagcacg ttgtgcgctt gagcaggggc gtgaagtttt
     4681 tgccttgcca ggtccaatag ggaatccggg aagcgaaggg cctcactggt taataaaaca
     4741 aggtgcgatt cttgtgacgg aaccggaaga aattctggaa aacttgcaat ttggattgca
     4801 ctggttgcca gacgcccctg aaaattcatt ttattcacca gatcagcaag acgtggcatt
     4861 gccatttcct gagctcctgg ctaacgtagg agatgaggta acacctgttg acgtcgtcgc
     4921 tgaacgtgcc ggccaacctg tgccagaggt agttactcaa ctactcgaac tggagttagc
     4981 aggatggatc gcagctgtac ccggcggcta tgtccgattg aggagggcat gccatgttcg
     5041 acgtactaat gtatttgttt gaaacctata ttcacactga agctgagttg cgtgtggatc
     5101 aagacaaact tgaacaggat cttaccgacg caggttttga gcgagaagat atctacaatg
     5161 ccctgctatg gctggaaaaa cttgctgatt atcaggaagg gttggcagaa ccgatgcaac
     5221 tggcctcgga tcctctctcc atgcgtattt atacaccgga agagtgtgaa cgactggatg
     5281 ccagctgccg tgggtttctg cttttccttg agcagattca ggtgctcaac cttgaaactc
     5341 gtgaaatggt gatagagcga gtgctcgcgc tggataacgc agagttcgaa ctggatgatc
     5401 tgaaatgggt gatcctgatg gtgttgttca acatcccggg ctgcgaaaat gcgtaccagc
     5461 aaatggaaga attactcttt gaagtgaatg aaggtatgct gcattaatca tttctttaat
     5521 tcagcataag ttgttatggc aaaatcagca ctgttcacgg tgcgtaataa tgagtcctgc
     5581 ccaaagtgcg gggctgaact ggttattcga tccgggaaac acggtccgtt tcttggatgc
     5641 tcacagtatc cggcgtgtga ctacgtccgt cctctgaaat cttcagcgga tggacatatt
     5701 gtcaaagttc tggaggggca ggtttgcccc gtatgtggcg caaatctggt attacgccag
     5761 ggacgctttg gtatgtttat tggttgcagt aactaccctg aatgcgaaca taccgaactt
     5821 atcgataaac cggacgaaac agcaattaca tgcccccaat gtcggacggg ccatctggtc
     5881 cagcgccgct cccgttatgg caaaacattt cactcctgtg atcgctaccc ggagtgtcaa
     5941 tttgccatta acttcaaacc aatagctgga gaatgccctg agtgtcatta tccgctactc
     6001 atcgaaaaga aaaccgcgca gggtgtaaaa cacttttgtg ccagtaaaca atgtggaaag
     6061 ccggtttcgg cggaataata acgtgaataa taacctgcaa ggagacgcta tcgcagctgc
     6121 gatagatgtc ctcaatgaaa aacgtgtcat cgcctatcca acggaagccg ttttcggtgt
     6181 cgggtgcgat cctgatagcg aaacagcagt gatgagactg ttggagttaa aacagcgtcc
     6241 ggttgataag gggctgattt taatcgcggc aaattacgag cagcttaaac cctatattga
     6301 tgacaccatg ttgactgacg tgcagcgtga aaccattttt tcctgctggc caggtcctgt
     6361 cacctttgtc tttcccgcgc ctgcgacaac accgcgctgg ttgacgggcc gctttgattc
     6421 gcttgctgta cgagtcaccg accatccgtt ggtggttgct ttgtgccagg cttatggtaa
     6481 accgctggtt tctaccagtg ccaacttgag tggattgcca ccttgtcgaa cagtagacga
     6541 agttcgcgca caatttggcg cggcgttccc ggttgtgcct ggtgaaacgg gggggcgttt
     6601 aaatccttca gaaatccgcg atgccctgac gggtgaactg tttcgacagg ggtaacataa
     6661 tggaaaccta tgctgttttt ggtaatccga tagcccacag caaatcgcca ttcattcatc
     6721 agcaatttgc tcagcaactg aatattgaac atccctatgg gcgcgtgttg gcacccatca
     6781 atgatttcat caacacactg aacgctttct ttcgtgctgg tggtaaaggt gcgaatgtga
     6841 cggtgccttt taaagaagag gcttttgcca gagcggatga gcttactgaa cgggcagcgt
     6901 tggctggtgc agttaatacc ctcaagcggt tagaagatgg acgcctgctg ggtgacaata
     6961 ccgatggtgt aggcttgtta agcgatctgg aacgtctgtc ttttatccgc cctggtttac
     7021 gtattctgct tatcggcgct ggtggagcat ctcgcggcgt actactgcca cttctttccc
     7081 tggactgtgc ggtgacaatc actaatcgga cggtatcccg cgcggaagag ttggctaaat
     7141 tgtttgcgca cactggcagt attcaggcgt tgggtatgga cgaactggaa ggtcatgagt
     7201 ttgatctcat tattaatgca acatccagtg gcatcagtgg tgatattccg gcgatcccgt
     7261 catcgctcat tcatccaggc atttattgct atgacatgtt ctatcagaaa ggaaaaactc
     7321 cttttctggc atggtgtgag cagcgaggct caaagcgtaa tgctgatggt ttaggaatgc
     7381 tggtggcaca ggcggctcat gcctttcttc tctggcacgg tgttctgcct gacgtagaac
     7441 cagttataaa gcaattgcag gaggaattgt ccgcgtgaat caggccatcc agtttccgga
     7501 cagggaagag tgggacgaga ataaaaaatg tgtatgtttt cccgctctcg tgaatggtat
     7561 gcaactgaca tgcgcgatct ctggcgagag tctggcgtat cgctttactg gagatacgcc
     7621 agaacagtgg ttagcgagtt ttcgtcagca tcgctgggac ctggaagaag aagcggaaaa
     7681 cttaattcag gaacaaagtg aagatgatca aggctgggtc tggttaccct gatccagata
     7741 ttcgtccttc catttcacgt aattattcgc ggaatagcgt aacccagcct tctcttcatc
     7801 acttaacggg cggatctgtt tgacggggct accgagatac agatatccgc tctccagccg
     7861 tttattttgt gggaccagac tacccgcacc aatcatcaca tcatcttcta ctattgcgcc
     7921 atcaagtaaa attgagccca tcccaaccaa aactcgattg ccaatggtgc agccgtggag
     7981 catcaccttg tgaccaacag tgacatcttc gccaatggtt aatgggttgc catctgggtt
     8041 gtacgaggat ttatgagtga catgcaacat actgccatcc tggatattgg tgcgtgctcc
     8101 gatctgtaca taatgtacat ctccacgaat cacaacgagc ggccagatcc ccacatcatc
     8161 agccagacga acgtcaccaa tcacgacact gctatcgtcg atcattacgc gctgaccgat
     8221 ttgtggaaaa agatcgcggt atgggcgtaa aacatcagac atacttacct cagcaataaa
     8281 tgatttacta atgactttgg gggcattatt ggccttgtgc aagtctttta gtatgcaaaa
     8341 aagcaccgtt ttgtgtgcga ttgcagcaaa aagggtgaaa aaacaacaaa cagaaaaaaa
     8401 gatcaaaaaa atacttgtgc aaaatattgg gatccctata atgcgcctcc gttgagacga
     8461 caacgtgaaa cacttcacag gatggtcgga acaacgaaga g
//
LOCUS       DAJVQH010000146         8381 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJVQH010000146 DAJVQH010000000
VERSION     DAJVQH010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.146"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(70..861)
                     /gene="aadA1"
                     /locus_tag="OQM87_003590"
     CDS             complement(70..861)
                     /gene="aadA1"
                     /locus_tag="OQM87_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1509573.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(954..1427)
                     /locus_tag="OQM87_003591"
     CDS             complement(954..1427)
                     /locus_tag="OQM87_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS1509574.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1587..2600
                     /gene="intI1"
                     /locus_tag="OQM87_003592"
     CDS             1587..2600
                     /gene="intI1"
                     /locus_tag="OQM87_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCS1509575.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2803..3153
                     /locus_tag="OQM87_003593"
     CDS             2803..3153
                     /locus_tag="OQM87_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCS1509576.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3279..3839
                     /locus_tag="OQM87_003594"
     CDS             3279..3839
                     /locus_tag="OQM87_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1509577.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3842..6808
                     /locus_tag="OQM87_003595"
     CDS             3842..6808
                     /locus_tag="OQM87_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCS1509578.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLEGAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6875..7252
                     /locus_tag="OQM87_003596"
     CDS             6875..7252
                     /locus_tag="OQM87_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1509579.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7453..8112)
                     /locus_tag="OQM87_003597"
     CDS             complement(7453..8112)
                     /locus_tag="OQM87_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCS1509580.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1847 a         2437 c         2283 g         1814 t
ORIGIN      
        1 cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc ttgaacgaat
       61 tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt gaacaaattc
      121 ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat aagcctgtct
      181 agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc
      241 gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag
      301 cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc
      361 atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg
      421 acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc
      481 gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg
      541 cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac
      601 ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt
      661 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
      721 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
      781 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
      841 ggcgatcacc gcttccctca tgatgtttaa cgcctggcac agcggatcgc aaacctggcg
      901 cggcttttgg tacaaaaggc gtgacaggtt tgcgaatccg ttgctgccac ttgttaaccc
      961 ttttgccaga tttggtaact ataatttatg ttagaggcga agtcttgggt aaaaactggc
     1021 ctaaaattgc tggggatttc aggaaagtaa acatcacctt ccggctcgat gtctattgta
     1081 gatatatgta gtgtatctac ttgatcgatc aggcttttgt atatctcccc accacctgaa
     1141 acaatgacat gatccgttat tttctttagg ttggttaaag catctttaat tgatggaaag
     1201 atcactacgt tctcattgtc agatgtaaaa cttgaacgtg ttacgaccgc atactttcgg
     1261 ttgggtaatg ctcccattga ttcaaaagtc ttgcgtccaa ccaacagcca ttggttatag
     1321 gtaatagctt taaacaggag ctgttcacct ttggcactcc atggaatatc agggccattc
     1381 ccgataactc cattcttcga tatagctacc attagtgata gtttcacaat tcttcctcag
     1441 aggttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca
     1501 tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta
     1561 ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
     1621 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac
     1681 gaaccgaaca ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
     1741 acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc
     1801 gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca
     1861 aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc
     1921 gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg
     1981 agcatcgttt gttcgcccag cttctgtatg gaacgggcat gcggatcagt gagggtttgc
     2041 aactgcgggt caaggatctg gatttcgatc acggcacgat catcgtgcgg gagggcaagg
     2101 gctccaagga tcgggccttg atgttacccg agagcttggc acccagcctg cgcgagcagc
     2161 tgtcgcgtgc acgggcatgg tggctgaagg accaggccga gggccgcagc ggcgttgcgc
     2221 ttcccgacgc ccttgagcgg aagtatccgc gcgccgggca ttcctggccg tggttctggg
     2281 tttttgcgca gcacacgcat tcgaccgatc cacggagcgg tgtcgtgcgt cgccatcaca
     2341 tgtatgacca gacctttcag cgcgccttca aacgtgccgt agaacaagca ggcatcacga
     2401 agcccgccac accgcacacc ctccgccact cgttcgcgac ggccttgctc cgcagcggtt
     2461 acgacattcg aaccgtgcag gatctgctcg gccattccga cgtctctacg acgatgattt
     2521 acacgcatgt gctgaaagtt ggcggtgccg gagtgcgctc accgcttgat gcgctgccgc
     2581 ccctcactag tgagaggtag ggcagcgcaa gtcaatcctg gcggattcac tacccctgcg
     2641 cgaaggccat cggtgccgca tcgaacggcc ggttgcggaa agtcctccct gcgtccgctg
     2701 atggccggca gcagcccgtc gttgcctgat ggatccaacc cctccgctgc tatagtgcag
     2761 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga caatggaggt ggtagccgag
     2821 ggtgtggaaa cacccgactg ccttgcgtgg ttgcggcagg cgggttgcga cacggtgcag
     2881 ggtttcctgt tcgccaggcc gatgccggcg gcggccttcg tcggcttcgt caaccaatgg
     2941 aggaacacca ccatgaacgc caatgaaccg agcaccagtt gctgcgtgtg ctgcaaggaa
     3001 atcccgctcg atgccgcctt cacgccggaa ggggccgagt acgtggagca tttctgcggg
     3061 ctggagtgct atcagcgctt ccaggcgcgg gccagcactg cgaccgaaac cagcgtcaaa
     3121 ccggacgctt gtgattcgcc gccgtcaggt tgaggcatac cctaacctga tgtcagatgc
     3181 catgtgtaaa ttgcgtcagg ataggattga attttgaatt tattgacata tctcgttgaa
     3241 ggtcatagag tcttccctga cattttgcag ggaattccat gactggacag cgcattgggt
     3301 atatcagggt cagcaccttc gaccagaacc cggaacggca actggaaggc gtcaaggttg
     3361 atcgcgcttt tagcgacaag gcatccggca aggatgtcaa gcgtccgcaa ctggaagcgc
     3421 tgataagctt cgcccgcacc ggcgacaccg tggtggtgca tagcatggat cgcctggcgc
     3481 gcaatctcga tgatttgcgc cggatcgtgc aaacgctgac acaacgcggc gtgcatatcg
     3541 aattcgtcaa ggaacacctc agttttactg gcgaagactc tccgatggcg aacctgatgc
     3601 tctcggtgat gggcgcgttc gccgagttcg agcgcgccct gatccgcgag cgtcagcgcg
     3661 agggtattgc gctcgccaag caacgcgggg cttaccgtgg caggaagaaa tccctgtcgt
     3721 ctgagcgtat tgccgaactg cgccaacgtg tcgaggctgg cgagcaaaag accaagcttg
     3781 ctcgtgaatt cggaatcagt cgcgaaaccc tgtatcaata cttgagaacg gatcagtaaa
     3841 tatgccacgt cgttccatcc tgtccgccgc cgagcgggaa agcctgctgg cgttgccgga
     3901 ctccaaggac gacctgatcc gacattacac attcaacgat accgacctct cgatcatccg
     3961 acagcggcgc gggccagcca atcggctggg cttcgcggtg cagctctgtt acctgcgctt
     4021 tcccggcgtc atcctgggcg tcgatgaact accgttcccg cccttgttga agctggtcgc
     4081 cgaccagctc aaggtcggcg tcgaaagctg gaacgagtac ggccagcggg agcagacccg
     4141 gcgcgagcac ctgagcgagc tgcaaaccgt gttcggtttc cggcccttca ccatgagcca
     4201 ttaccggcag gccgtccaga tgctgaccga gctggcgatg caaaccgaca aaggcatcgt
     4261 gctggccagc gccttgatcg ggcacctgcg gcggcagtcg gtcattctgc ccgccctcaa
     4321 cgccgtcgag cgggcgagtg ccgaggcgat cacccgtgct aaccggcgca tctacgacgc
     4381 cttggccgaa ccactggcgg acgcgcatcg ccgccgcctc gacgatctgc tcaagcgccg
     4441 ggacaacggc aagacgacct ggttggcttg gttgcgccag tctccggcca agccaaattc
     4501 gcggcatatg ctggaacaca tcgaacgcct caaggcatgg caggcactcg atctgcctac
     4561 cggcatcgag cggctggttc accagaaccg cctgctcaag attgcccgcg agggcggcca
     4621 gatgacaccc gccgacctgg ccaaattcga gccgcaacgg cgctacgcca ctctcgtggc
     4681 gctggccacc gagggcatgg ccaccgtcac cgacgaaatc atcgacctgc acgaccgcat
     4741 cctgggtaag ctgtttaacg ctgccaagaa taagcatcag cagcagttcc aggcgtcagg
     4801 caaggccatc aacgccaagg tacgtctgta cgggcgcatc ggtcaggcgc tgatcgacgc
     4861 caagcaatca ggccgcgatg cgtttgccgc catcgaggcc gtcatgtcct gggattcctt
     4921 tgccgagagc gtcaccgagg cgcagaagct cgcgcaaccc gatgacttcg atttcctgca
     4981 tcgcatcggc gagagctacg ccaccctgcg ccgctatgca ccggaattcc ttgccgtgct
     5041 caagctgcgg gccgcgcccg ccgccaaaaa cgtgcttgat gccattgagg tgctgcgcgg
     5101 catgaacacc gacaacgccc gcaagctgcc agccgatgca ccgaccggct tcatcaagcc
     5161 gcgctggcag aaactggtga tgaccgacgc cggcatcgac cggcgctact acgaactgtg
     5221 cgcgctgtcc gagttgaaga actccctgcg ctcgggcgac atctgggtgc agggttcacg
     5281 ccagttcaag gacttcgagg actacctggt accgcccgag aagttcacca gcctcaagca
     5341 gtccagcgaa ttgccgctgg ccgtggccac cgactgcgaa caatatctgc atgagcggct
     5401 gacgctgctg gaagcacaac ttgccaccgt caaccgcatg gcggcagcca acgacctgcc
     5461 ggatgccatc atcaccgagt cgggcttgaa gatcacgccg ctggatgcgg cggtgcccga
     5521 caccgcgcag gcgctgatag accagacagc catggtcctg ccgcacgtca agatcaccga
     5581 actgctgctc gaagtcgatg agtggacggg cttcacccgg cacttcacgc acttgaaatc
     5641 gggcgatctg gccaaggaca agaacctgtt gttgaccacg atcctggccg acgcgatcaa
     5701 cctgggcctg accaagatgg ccgagtcctg ccccggcacg acctacgcga agctcgcttg
     5761 gctgcaagcc tggcataccc gcgacgaaac gtactcgaca gcgttggctg aactggtcaa
     5821 cgctcagttt cggcatccct ttgccgggca ctggggcgat ggcaccacat catcatcgga
     5881 cggacagaat ttccgaaccg ctagcaaggc aaagagcacg gggcacatca acccaaaata
     5941 tggcagcagc ccaggacgga ctttctacac ccacatctcc gaccaatacg cgccattcca
     6001 caccaaggtg gtcaatgtcg gcctgcgcga ctcaacctac gtgctcgacg gcctgctgta
     6061 ccacgaatcc gacctgcgga tcgaggagca ctacaccgac acggcgggct tcaccgatca
     6121 cgtcttcgcc ctgatgcacc tcttgggctt ccgcttcgcg ccgcgcatcc gcgacctggg
     6181 cgacaccaag ctctacatcc cgaagggcga tgccgcctat gacgcgctca agccgatgat
     6241 cggcggcacg ctcaacatca agcacgtccg cgcccattgg gacgaaatcc tgcggctggc
     6301 cacctcgatc aagcagggca cggtgacggc ctcgctgatg ctcaggaaac tcggcagcta
     6361 cccgcgccag aacggcttgg ccgtcgcgct gcgcgagttg ggccgcatcg agcgcacgct
     6421 gttcatcctc gactggctgc aaagcgtcga gctacgccgc cgcgtgcatg ccgggctgaa
     6481 caagggcgag gcgcgcaatg cgctggcccg tgccgtgttc ttcaaccgcc ttggtgaaat
     6541 ccgtgaccgc agtttcgagc agcagcgcta ccgggccagc ggcctcaacc tggtgacggc
     6601 ggccatcgtg ctgtggaaca cggtctacct ggagggtgcg gcgcatgcgt tgcgcggcaa
     6661 tggtcatgcc gtcgatgact cgctattgca gtacctgtcg ccactcggct gggagcacat
     6721 caacctgacc ggtgattacc tatggcgcag cagcgccaag atcggcgcgg ggaagttcag
     6781 gccgctacgg cctctgcaac cggcttagcg tgctttattt tccgttttct gagacgaccc
     6841 ctattatggc gaaggtatca ttcaggaagc gttgatgaag gattatctct gtaaaaagtt
     6901 attcaaccgg ctttccggta ccctggtgat cagagcgcgc tgcggcaata acattactgg
     6961 cctggcatgt tgcaatattc tttatccctc gccccgatac agcggtcagc tgcatattaa
     7021 agagctgtat gtttctcagt gcgatcgaaa taagggcacg ggtaaagcga taatgcgctt
     7081 tatagcccgg cttgcgcttg aacaggaatg ccttagcctt agctggaacg ctgaaaaatc
     7141 caaccccggc gctaaccgtt tttatcaggc tctgggaggc agaataaatg atcatatcgt
     7201 caattattac ctccacgggg agagcctgag caaactggcc tcaggcattt gagaagcaca
     7261 cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta
     7321 tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt
     7381 catccgctta ttatcactta ttcaggcgta gcaaccaggc gtttaagggc accaataact
     7441 gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     7501 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     7561 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     7621 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     7681 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     7741 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     7801 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     7861 tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg
     7921 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     7981 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     8041 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     8101 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     8161 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc
     8221 tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac caggatttat
     8281 ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga
     8341 tgctgccaac ttactgattt agtgtatgat ggtgtttttg a
//
LOCUS       DAJVQH010000147         8349 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJVQH010000147 DAJVQH010000000
VERSION     DAJVQH010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.147"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..157
                     /locus_tag="OQM87_003598"
     CDS             <1..157
                     /locus_tag="OQM87_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509581.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(166..564)
                     /gene="vapC"
                     /locus_tag="OQM87_003599"
     CDS             complement(166..564)
                     /gene="vapC"
                     /locus_tag="OQM87_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCS1509582.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(564..791)
                     /gene="vapB"
                     /locus_tag="OQM87_003600"
     CDS             complement(564..791)
                     /gene="vapB"
                     /locus_tag="OQM87_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCS1509583.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            873..1292
                     /locus_tag="OQM87_003601"
     CDS             873..1292
                     /locus_tag="OQM87_003601"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCS1509584.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTGDDGRTVRNGADR"
     gene            1279..6144
                     /gene="traI"
                     /locus_tag="OQM87_003602"
     CDS             1279..6144
                     /gene="traI"
                     /locus_tag="OQM87_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCS1509585.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQYNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISSEQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQVETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIA
                     PGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPR
                     LEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEA
                     REQTLIVTHLNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRLSTWE
                     NNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRV
                     GTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQAYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPT
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQA
                     VREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVR
                     DLQKEKTPGGD"
     gene            6164..6910
                     /gene="traX"
                     /locus_tag="OQM87_003603"
     CDS             6164..6910
                     /gene="traX"
                     /locus_tag="OQM87_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCS1509586.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6965..7525
                     /locus_tag="OQM87_003604"
     CDS             6965..7525
                     /locus_tag="OQM87_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCS1509587.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPWLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7656..7868
                     /locus_tag="OQM87_003605"
     CDS             7656..7868
                     /locus_tag="OQM87_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCS1509588.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8112..>8349
                     /locus_tag="OQM87_003606"
     CDS             8112..>8349
                     /locus_tag="OQM87_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCS1509589.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1916 a         2095 c         2673 g         1665 t
ORIGIN      
        1 cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcagc aggaaaatca
       61 tccggacacc tggcagcaga tgcagcgtcg tgaagaggtg aacatcaatg tgcaccggga
      121 acgcggggag gatgttgagc cgggagatga tttctgatga acaggtcagc tccagtcttc
      181 agttctcagg ccgcccacac gttcaaattc ccgggtgtta ttagtcacaa taatcagccc
      241 ccgactgcgg gcatgacctg cgatcatttg atcaaatggc ccgacagggc gtccctgacg
      301 ggcaagttct gctcttatct ggccggtgtg tgtggctgca gcagcgtcgt aatccagaac
      361 atcaatgcgg gaaacaaatc cctcgatcac agcgagatta cgttcaggca tctggctttt
      421 ttctgcacca tatatcagct ccatcagggt gaccgaactg atacacattt tcccctggtt
      481 caggttaaaa cgctccctga cgctggcggg tttgttcttt atcgtaaaaa tgcagatgtt
      541 ggtatcgagc ataaacttca gcatcagaat gactcccttt cctgcatgcc gggctgttcc
      601 ctgttatcca taaaatcggt gctgacgctg tgtccgtcga accattcgtc ccatgtctct
      661 ccggctggcg taatgattct ggttcgtccg acagcaatca cttcaacgcg ttttacgttt
      721 tccggcaatg caaccgcttt tggcagtctg accgcctggc tgcggttgct gagaaatacg
      781 gtggtttcca ttatttgcct ccttatgtgg atatgtcttg tgtatatcta ttgtgggatt
      841 gataccggga tatgtcaaag ggatatacgt ttatgatgag tatcgcgcag gtcagatcgg
      901 ccggaagtgc cggtaattat tataccgaca aggataatta ctatgtgctg ggcagcatgg
      961 gagaacgctg ggccggccag ggggctgaac aactggggct gcagggcagt gtcgataagg
     1021 atgtctttac ccgccttctg gagggcaggc tgccggacgg ggcggattta agccgcatgc
     1081 aggatggcag taacaggcat cgtcccggct acgacctgac cttctccgct cccaaaagta
     1141 tctccatgat ggccatgctc gggggggaca aacgtcttat tgaagcacat aaccaggccg
     1201 tggattttgc tgtccgtcat cacccgtgtg gaggcactgg cctccacacg ggtgatgacg
     1261 gacggacagt cagaaacggt gctgaccggt aatctggtga tggcactgtt taaccacgac
     1321 accagtcgcg atcaggaacc acagttacac acgcatgcgg tggtgactaa tgttacgcag
     1381 tataacggcg aatggaagac actgagcagt gacaaagtgg ggaaaacggg gttcattgag
     1441 aatgtgtacg ctaatcagat tgcctttggc aggctctacc gggaaaaact gaaagagcag
     1501 gttgaggcgc tgggctatga aactgaagtg gtcggtaagc acggtatgtg ggaaatgccg
     1561 ggtgtaccgg tggaggcctt ttccggacgc tcacagacta tccgggaggc cgtcggggaa
     1621 gacgcctcgc tgaaatcccg ggatgtggcg gcgctggata cgcgtaaatc caaacagcac
     1681 gtcgatcctg aagtcagaat ggccgagtgg atgcagacgc tgaaggaaac cgggttcgac
     1741 atccgggcat atcgtgacgc agcggaacaa cgggcatata cccgcacgca gacacccgga
     1801 cccgcttcac aggacgggcc ggatgtgcag caggcggtga cacaggccat tgccggatta
     1861 agtgaacgca aagtgcagtt tatgtatacg gacctgctgg ccaggacggt cggcatactg
     1921 ccgccggaaa atggtgtgat tgaacgggca cgcgccggta tcgatgaggc catcagcagt
     1981 gagcagctta tccccctcga tcgtgagaag gggctgttca cgtccgggat tcatatgctc
     2041 gatgagctgt cagtccgggc actcagtcgt gacatcatga aacagaaccg ggtgaccgta
     2101 catccggaga aaagtgtccc ccggacggcc ggttacagcg atgccgtcag tgttctggca
     2161 caggaccgtc cgtcgctggc cattgtgtcc gggcagggcg gtgcagccgg gcagcgtgag
     2221 cgggtggctg aactggtcat gatggcccgg gagcaggggc gggaggtgca gattatcgct
     2281 gctgaccgtc gctcgcagat gaacctgaag caggatgaac ggctgtccgg tgaactgata
     2341 accggacgtc gtcagttgct ggaaggcatg gccttcacgc cgggcagtac cgttatcgtc
     2401 gaccagggcg aaaaactctc cctgaaagag acgttaaccc tgctggatgg tgccgcacgt
     2461 cataacgtac aggtcctgat aaccgacagc gggcagcgaa ccggtacagg cagtgcactg
     2521 atggccatga aggatgccgg ggtgaacaca tatcgctggc aggggggaga acagcgaccg
     2581 gccaccatca tcagtgaacc ggaccgtaat gtccgctatg cccggctggc cggagatttt
     2641 gcggccagcg tgaaagccgg agaagagagc gtggcacagg tcagcggagt acgggaacag
     2701 gccatactga cacaggccat tcgcagtgag ctgaaaacac agggcgtgct cggacacccg
     2761 gaggtaacca tgaccgccct gtcaccggtc tggctggaca gccggagccg ttatctgcgg
     2821 gatatgtacc gcccgggaat ggtgatggag cagtggaacc cggagacacg cagtcatgac
     2881 cgctatgtga cagaacgtgt gacggcgcag agtcacagcc tgaccctgcg gaatgcgcag
     2941 gtcgaaacgc aagtggtgcg tatttcttcc ctggacagca gctggtcgct gttccggccg
     3001 gaaaaaatgc cggtggcaga cggtgagcgt ctgagggtga cagggaaaat tcccggactc
     3061 cgcgtctccg gcggtgaccg cctgcaggtg gcatccgtca gtgaagatgc gatgacggtt
     3121 gttgtgccgg gacgggctga accggccacc ctgcctgtga gcgattcacc gttcacggca
     3181 ctgaagctgg agaacggctg ggtggaaacg cccgggcatt ccgtcagcga cagtgcgacg
     3241 gtttttgcct ccgtcacaca gatggcaatg gacaatgcca ccctgaatgg tctggcccgc
     3301 agtggtcgtg atgtccggct gtattcctca ctggatgaaa cccgtactgc ggaaaaactt
     3361 gcccgccatc cctcctttac ggtggtttct gagcagataa aggcgcgtgc cggtgagaca
     3421 ttgctggaaa ccgctatcag tctgcagaaa gccgggcttc acacgccggc acagcaggcc
     3481 attcatctgg cccttcctgt gctggaaagt aaaaacctgg ccttcagcat ggtggacctg
     3541 ctgacagagg cgaagtcgtt tgctgcagaa ggaaccggtt ttgctgacct gggaggggaa
     3601 atcaatgcgc agataaaacg gggtgattta ctgtatgtgg atgtggcaaa aggctatggc
     3661 acaggcctgc tggtttcccg tgcgtcgtat gaggcagaaa agagcattct tcgccatatt
     3721 ctcgaaggta aggaggcggt cacgccgctg atggagagag tacctggcga actcatggag
     3781 aaactgacat caggacagcg cgccgccacc cgtatgatac tggaaacgtc tgaccgtttc
     3841 acggtggtgc agggctatgc cggtgtgggt aagaccacac agttccgggc ggtgatgtca
     3901 gccgtgaaca tgctgccgga gagtgaacgt ccccgtgtcg tggggctggg gcccacacac
     3961 cgtgcggtcg gggagatgcg cagcgccggc gtggatgcac agacactggc gtcctttctg
     4021 catgacacgc agcagcgcag cggagaaacg ccggatttca gcaacacgct gttccttctc
     4081 gatgagagct ctatggtggg caataccgac atggcacggg catacgccct gattgcggcc
     4141 ggtggcggtc gggctgtggc cagcggtgac acggaccagc tgcaggccat cgcgcccggt
     4201 cagcctttcc gtctccagca gacgcgcagt gctgccgatg tggtcatcat gaaggagatt
     4261 gtgcgtcaga cgccggaact gcgggaggcg gtatacagcc tgattaaccg ggatgtggaa
     4321 agggcgctgt ccgggcttga gcgtgtgaaa ccgtctcagg tgccacgtct ggagggcgca
     4381 tgggcaccgg agcactccgt gacggagttc agtcacagcc aggaagcgaa actggcagaa
     4441 gcgcagcaga aggcgatgct gaaaggcgag gcttttccgg atgtccccat gacactgtat
     4501 gaagccattg tccgcgacta taccggcagg acaccggaag cacgggagca gacgctgatt
     4561 gtcacgcacc tgaatgagga ccggcgcgta ctgaacagca tgattcatga tgcacgggaa
     4621 aaggccggag aactggggaa ggtgcaggtc atggtgcctg tcctgaacac agcgaatata
     4681 cgcgacgggg agctgcgtcg tctctccacc tgggagaata acccggatgc ccttgccctg
     4741 gtggataatg tgtatcaccg gattgccggt atcagcaagg atgacgggct gataaccctg
     4801 caggatgcgg aaggtaacac gcggctgatt tcgccccggg aggcggtggc tgaaggcgtc
     4861 acgctgtaca ccccggacac catccgggtg gggaccggtg acaggatacg cttcacgaag
     4921 agtgaccggg agcgcggtta tgtggccaac agcgtctgga cggtgacagc ggtttccggt
     4981 gacagtgtca cactttctga cggtcagcag acccgtgtga ttcgccccgg tcaggagcgg
     5041 gcagagcaac atattgacct ggcctatgcc atcaccgccc acggtgcaca gggggcgagt
     5101 gaaacctttg ccatcgcgct tgaaggcacg gaaggtaacc ggaaactgat ggccggcttt
     5161 gagtcagcct acgtggccct gtcgcgtatg aagcagcatg tgcaggcgta caccgataac
     5221 cgtcagggct ggacggatgc catcaacaat gccgtacaga aaggaactgc ccacgatgtg
     5281 tttgagccga aaccggaccg ggaggtcatg aatgcagagc ggctgttcag tacggcgcgg
     5341 gagctgcggg acgtggcggc aggccgtgcc gttctccgtc aggcggggct ggccggggga
     5401 gacagtccta cacggtttat tgctccggga cgtaaatatc cgcaaccgta tgtggcactg
     5461 ccggcgtttg accgtaacgg caagtccgcc ggtatctggc tgaacccgct gaccacggat
     5521 gacggaaacg ggctgcgggg attcagcggt gaaggtcggg taaaaggcag cggggatgcg
     5581 cagttcgtgg ccctgcaggg cagccgtaac ggagaaagcc tgctggctga taatatgcag
     5641 gatggcgtcc ggattgcccg tgataatcct gacagtggtg tggtggtaag aatcgccggt
     5701 gaaggccgtc cgtggaatcc cggtgccatc accggtggcc gcgtgtgggg ggatatcccg
     5761 gacaacagcg tccagccggg agccggaaat ggcgaaccgg tcacggcaga ggtactggca
     5821 cagcggcagg ctgaagaggc catccgccgt gaaacggaac gtcgcgcaga tgaaattgtc
     5881 cgtaaaatgg cagagaacaa acctgacctg ccggacggca aaacagagca ggctgtcagg
     5941 gaaattgccg gtcaggagcg tgaccgggct gccataactg aacgggaagc cgcgctgccg
     6001 gagagtgtac tgcgtgaacc acaacgggtg cgggaagcgg tccgggaagt tgcccgggaa
     6061 aatttgctgc aggagcgtct gcagcagatg gagcgggata tggttcgtga cctgcagaaa
     6121 gagaaaaccc cgggcggaga ctgatacagg aagacaaact ctgatgacaa cggataacac
     6181 gaacacgaca cgtaacgatt cactggctgc acggaccgat acctggttgc agtcatttct
     6241 ggtctggtca cccggacagc gggatatcat caaaacggtg gcactggtgc tgatggtact
     6301 ggaccacatc aatctgatat tccagctgaa gcaggaatgg atgtttctgg ccgggcgagg
     6361 agcctttccg ctttttgccc tggtgtgggg gctgaatctg tcccgtcatg cgcatatccg
     6421 gcaaccggcc attaaccggc tgtggggatg gggaattatt gcccagttcg catattatct
     6481 ggcaggcttt ccttggtatg aggggaatat cctgtttgcc tttgcagtgg cggcacaggt
     6541 gctgacgtgg tgtgaaacgc gcagtgggtg gcgtacagca gcggccatac tcctgatggc
     6601 cctgtggggg cctttgtccg gcaccagtta cggtattgcc gggttgctga tgctggcagt
     6661 cagcaaccgg ctgtaccggg cggaagacag agcggaacgt ctggcgctgg tcgcctgcct
     6721 gctggctgtt attccggcgc ttaaccttgc caccagtgat gcagcggcgg tggccggtct
     6781 tgtgatgacg gtgctgaccg ttggtctggt gttgtgtgcc ggaaaatcat taccccgttt
     6841 ctggcccgga gactttttcc cgacgttcta tgcctgtcat ctggctgtgc tgggcgttct
     6901 ggcgctgtga cgggtgtggt atctttggcc gcaagaggat gattcgtcag aggcagaaca
     6961 cagcatgata gagcagaagc gaccggtact gacactgaag cggaagacag agggaacagc
     7021 gcctgtccgc agccggaaaa ccatcatcaa tgtcaccacg ccaccaaagt ggaaggtgaa
     7081 aaagcagaag ctggccgaga aagcggcccg tgaagcagag ctggcggcaa aaaaagcgca
     7141 ggccagacag gcgctgtcca tttatctgaa cctgcccacg ctggatgagg ctgtgaatac
     7201 cctgaagccc tggtggccgg gattatttga cggtgacacg ccctggcttc tggcctgcgg
     7261 tatccgggac gtgttactgg aagacgtggc gcagcggaat atcccgctct cgcataaaaa
     7321 actgcgcagg gcgctgaagg ccatcacccg ttcagaaagc tatctgtgtg ccatgaaagc
     7381 cggtgcctgc cggtatgaca cggaagggta tgtgacggag catatttctc aggaggagga
     7441 agcgtatgcg ggagcgcgtc tggcgaaaat tcgtcgccag aaccggataa aggcagaact
     7501 tcaggccgtg cttgatgaga aataaaaaaa gcctccccca gagagaggag gcagggaaat
     7561 aaggcttaaa aggaaataac ttccgcttcc gaaaggaagc cagtccactg aattatttat
     7621 cacaataata ctgacaacta aagggggaca agaaaatgaa tggattcaga aacagttcac
     7681 ggaacggcca ggtctggcgt taccagcgtg ccggcgggcg ggccgttatt ctggaagtca
     7741 gtggacgctg gatggaagcg gcagaagcat ggcgacgggc tgcctgtgtt gcgcctagga
     7801 cagactggca gcagtttgcc cggaaaaggg ctgaacactg ccaccggcgc tgccggggaa
     7861 gggtgtaacg aaaaaagcag ccttcattac tctaggagtc agaaggctga aaggagttta
     7921 tgagatttgc ttcttaatat ccggtcgggc agtatgaccg gacagttctg agtagcacag
     7981 ggtcttttcc gcattaagtc gattttttat attttgtgat taatttcaca aaataaggtg
     8041 ttattcaatg tgtgctgcac tattcagggg ggctgaaata caggtggcat ttatctgata
     8101 tggagaataa catgaggaaa tatattccac tggtattatt tatcttttca tggccggtat
     8161 tgtctgcaga tattcatgga cgggttgttc gtgtactgga cggtgatacc atcgaggtca
     8221 tggactcact gaaggcggtg cggatcaggc tggtaaatat tgatgcaccg gaaaagaaac
     8281 aggattatgg ccggtggtca acagacatga tgaaatcact ggtcgcagga aaaacggtta
     8341 ccgtcacat
//
LOCUS       DAJVQH010000148         8322 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJVQH010000148 DAJVQH010000000
VERSION     DAJVQH010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.148"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            336..3185
                     /locus_tag="OQM87_003607"
     CDS             336..3185
                     /locus_tag="OQM87_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1509590.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYATSRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQ
                     SKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIDSTHA
                     SPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNCVNANGT
                     ANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLL
                     GRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGWNNGVGV
                     SAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3285..5204)
                     /gene="dhaR"
                     /locus_tag="OQM87_003608"
     CDS             complement(3285..5204)
                     /gene="dhaR"
                     /locus_tag="OQM87_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCS1509591.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5432..6502
                     /gene="dhaK"
                     /locus_tag="OQM87_003609"
     CDS             5432..6502
                     /gene="dhaK"
                     /locus_tag="OQM87_003609"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCS1509592.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDVPVHTPALNWGK"
     gene            6513..7145
                     /gene="dhaL"
                     /locus_tag="OQM87_003610"
     CDS             6513..7145
                     /gene="dhaL"
                     /locus_tag="OQM87_003610"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCS1509593.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7156..>8322
                     /gene="dhaM"
                     /locus_tag="OQM87_003611"
     CDS             7156..>8322
                     /gene="dhaM"
                     /locus_tag="OQM87_003611"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCS1509594.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEE"
BASE COUNT         2174 a         2068 c         2076 g         2004 t
ORIGIN      
        1 atcccataac taattaggat tattacaaaa aatcttcacc aaatttttta accgctgttt
       61 gttgtaatat ttttgccata ttcatagtaa cgaattggaa taagtaacga caacttaggg
      121 aataactcga tataaactca cctttgtaag gggggtgtta aatatatatt aattcctgtg
      181 ttcctgattt tgcccggtaa tagagaatta ttctcagaga agctcatcat gattgcacca
      241 tacagatatc atgatgataa gaggatatta ttcattgact taaacaaaat gacaaaatcc
      301 atagacccat agcctgggta ctatatagga gtggtatggg catcaaacaa cacaatggga
      361 ataccaaagc cgatcgtctc gctgaattaa aaatccgttc gccctcaatt caactgataa
      421 aatttggcgc tattggtttg aatgcaatta tcttttcccc cctgctgata gctgctgata
      481 caggaagtca atatggcacc aatattacta ttaatgatgg tgacagaatt acaggagata
      541 ccgccgatcc atcaggaaac ctctatggtg taatgacccc agcaggaaac acgcctggca
      601 atatcaacct gggtaatgat gtcaccgtca atgtcaacga cgcctctgga tatgcaaaag
      661 gaatcattat tcagggcaaa aacagctccc tgacagctaa ccgactcaca gtagatgttg
      721 ttggtcaaac ctctgccatc ggcattaact taattggtga ctatacccat gctgacttag
      781 gcacaggcag caccattaag agtaacgatg acggcatcat tattgggcat agctcaacac
      841 taacagccac tcaattcacc attgaaaact cgaacggtat aggcctaacc atcaatgact
      901 atggcaccag tgtcgatctt ggaagcggaa gtaaaatcaa gaccgatgga agtacaggtg
      961 tttatatcgg tggtctcaac ggcaataacg ccaatggtgc tgcgcgtttt acggcgacag
     1021 acctgacaat cgatgttcag ggctacagcg ccatggggat aaacgtacag aaaaactctg
     1081 ttgtcgatct cggaacaaac agtaccatta aaaccaatgg cgataatgct cacggcctct
     1141 ggagctttgg ccaggttagc gcgaatgcac tcactgttga tgtaactgga gccgcggcca
     1201 atggcgtcga agttcgtggt ggtacaacca ctatcggtgc agatagccat atttcttccg
     1261 cgcagggcgg tggcctcgtc accagtggtt cagacgcgac aatcaatttt tctggcacgg
     1321 cagcgcaacg aaacagcatc ttttccggcg gttcttatgg tgcctcggcc cagacggcaa
     1381 cggctgttat caacatgcaa aataccgata ttacggttga tcgtaatggc agtctggcgc
     1441 tgggtttgtg ggcgctcagc ggcggtagaa taaccggaga cagtttggct atcaccggcg
     1501 cggcaggagc cagagggatt tatgccatga ccaacagcca gatcgacctc acgagcgatc
     1561 tggtcattga tatgagtaca cccgaccaga tggccatcgc aacgcaacat gacgatggtt
     1621 atgccaccag ccgcatcaac gcctcgggtc gtatgcttat caacggtagc gttctttcca
     1681 aaggtgggct aatcaatctg gatatgcacc ctgggtcggt ttggacaggt tcctccctca
     1741 gcgataatgt caatggcggg aaactggacg ttgcaatgaa taacagcgtc tggaacgtaa
     1801 caagtaattc taatctcgac acgctggcgc tgagccattc aactgtcgat tttgccagcc
     1861 acgggtcaac tgccggcaca tttaccacat taaacgtaga gaacctgagc ggtaacagta
     1921 cctttattat gcgtgctgat gttgttggcg agggtaatgg cgttaataat agaggggatt
     1981 tattgaatat cagcgggagt agtgctggta atcacgtatt ggctatccgc aaccagggca
     2041 gcgaggccac aacgggaaat gaagttctga cagtggtaaa aaccactgac ggcgcggcct
     2101 cgttcagcgc gtcttctcag gttgagttgg ggggatatct gtacgatgtg cgtaaaaatg
     2161 gcactaactg ggagctttac gcttccggga cagttccgga accgactcct aatcctgaac
     2221 ccacaccagc tcccgctcag cctcccatag tcaaccccga tcctacgcct aaacccacca
     2281 cgaccgcaga tgctggcggc aattatctca atgtcggtta cttattgaac tatgttgaaa
     2341 accgtacgct gatgcaacgg atgggtgact tgcgaaatca gagtaaagac ggtaatatct
     2401 ggttgcgcag ttatggggga agcctggact cctttgccag tggcaaactg agcggctttg
     2461 acatgggtta cagcggtatc cagtttggtg gggataaacg tctctctgat gtaatgccgt
     2521 tgtatgtcgg tctgtatatt gactcaacac atgcatcgcc ggactatagc ggaggcgacg
     2581 gtaccgcacg ttcagactac atgggaatgt acgccagtta catggcacaa aacggttttt
     2641 acagcgatct cgttataaaa gcatcgcgcc agaaaaatag tttccacgta ctggacagtc
     2701 agaacaactg cgttaacgcc aacggcactg cgaatggaat gagcatctcc ctggaagccg
     2761 ggcagaggtt caacctgtcc cctactggtt atgggttcta tatagagccg caaacccagc
     2821 ttacatacag ccaccagaat gagatggcta tgaaggcgag taatggcctc aatatacatc
     2881 tgaatcacta cgaatcgctg ctggggcgtg ccagcatgat actggggtat gacatcaccg
     2941 caggcaacag ccagctgaat gtctatgtga agactggcgc tatccgcgag ttttcagggg
     3001 ataccgaata tctgttgaac gactcccggg agaagtacag tttcaaaggt aatggctgga
     3061 ataacggcgt gggagtcagt gcacagtata acaaacagca cacattctat ctcgaagcgg
     3121 attacacgca gggtaacctc tttgatcaga agcaagtcaa cggaggatat cgcttcagct
     3181 tttaagtgcg ggatagacaa gcccttaaac gatgctgaaa attgttagcg tgcgccggaa
     3241 tgaaatacag atcactctga tgtatcagaa atcgtttctg tctttcatac ccggcgctta
     3301 aactgccctg catcaatgcc atgttgcttc attttccgcc acagcgtagt gcggccgatc
     3361 ccaagtaaag ccgacatttc ctgaatgcga ccgcctgtga cctgggctgc gttaataatt
     3421 gcctcttttt caacttccgc aaatgacaga ctggtggaaa ggcgggtggc gctgacatca
     3481 tctgtcgcct gctcggtaaa cagatgttca ggtaaatcac tgacgcgaat gcgcccgtta
     3541 tcactactca gagcaagatt ctcgatgacg ctgtaaagtt caaaatcgtt gcctggccat
     3601 gcacaagaaa ctaggcgagc gagggcatcg tcatcaattt tcagccgcgt agagaagcgt
     3661 ttttcaagac tgcgtaattt gttattcacc agcgccggaa tgctgccacg ccgcatacgc
     3721 agaggcggga tggtaatttc aaatgcatgc agcgcgtaat acagctggcg actaaaacga
     3781 ttttgttcca ccagcattgc gaggtccgcg gtcgttgttg caatcacttt gacatcaatt
     3841 ggtattaaac gccgcgcatc cagtcgcgtg ataaccccct gcttgataac ctgcagcaaa
     3901 gcagactgta actccaccgc cagatattca atcttttcaa gaaacagcgt gccgccgtgt
     3961 gccagttcca gccgactcag acggccattt tcattgtccg tgcgatcgcc accaataaat
     4021 tcttccgcca gcgcagcatc accatataac tcacaattga cggcgatata aggacctgca
     4081 gcacgctcgc tttcattatg aattgcctga cttagcagtg ccttgcccac gccctcttct
     4141 ccacaaagca ggacaggaaa gctactgcgc gccgcctggc gaccaaaatg aatcaagcgg
     4201 cgggtttgcg gatcgtcctg tggcatatga gcgaaggtat ggctgacttt tcctaattga
     4261 ctggtcatta actgccgcat ctgttccaca ggatggagca acaaaataaa gctggttccc
     4321 tgcgtttcta ttatcggttt aagggttatc accgcatcaa taaactggtg ctggctttca
     4381 aaggttgctt ctacgtgttt gagcggatgt gcctgtttta ttgcttgttg caatacggcg
     4441 ggtaacgtta agagttcagt gattgcccgt ccctgacttg ccgtcgcgtc aaggcgcaag
     4501 acccgcgccg cctgggcatt aataaattgc aaattaccct gctcgtccca gctaatcacg
     4561 ccatcatcca tactttctaa cagggcatta agttgattta aatgacggtt agtttcagcg
     4621 agcaaactgt ccgtcagcag taaattaccg acctcgcggg cgattgccaa cgtcaacggc
     4681 aaatcagctg cggtagtttg ctcaaccgga cacgccagcg ctattgttcc cgtcaatcgg
     4741 cccttgctgt caaacaacgg cgttgcacaa aaggcccagt tccagagtgc ctgtttgaaa
     4801 tgttgatcgg ccatcgtttt cacggcctga ccagagatag ccgctaacga tagcgcacaa
     4861 gtaccaataa ttccctcggc gcaatacgtg ccgtcattga atcccagtgc acttagctgc
     4921 tgcaaggttt gcggatcgcc attacggctg agaatgcagg cggtttcatc gaggataaac
     4981 agcgcacact ctcgcggtgc catatattcc caggcatctt ccagcgcagc ctgcccgagc
     5041 gtcagcatcg ctttcttacg ccgataaata gaagcaaatg tcacgccctg ggcctgatgt
     5101 ggtacgttcc atgtctcccg tttcatcagc ttattgcatc gctcccagga ggttgcaatt
     5161 aagggagata tgccccgacc atcgttgtta aaagcgccac tcatatcccg catcccttat
     5221 gacaaaaggt gttccggctc gcaattttaa gtattaatac acgtaaaagt ttcaatatgg
     5281 aacatttatt aaagcatatg tttcactatg aaacataatt tgggctaatt ttcttttccc
     5341 ttgcctgatg cacaatggat tcatcggcag tcagtggtcg ccgtgtcgtt gaacatcatc
     5401 catgccctac cgtaattgct ggcgcaaaat aatgaaaaaa ttgatcaatg atgtgcaaga
     5461 cgtactggac gaacaactgg caggactggc gaaagcgcat ccatcgctga cactgcatca
     5521 ggatccggtg tatgtcaccc gagctgatgc ccctgttgca ggaaaagtcg ccctgctgtc
     5581 gggtggcggc agcggacacg agccgatgca ctgtggttat atcggtcagg ggatgctttc
     5641 gggggcctgt ccgggcgaaa ttttcacctc accgacgccc gataaaatct ttgaatgctc
     5701 catgcaaatt gatggcggcg aaggtgtact gttgattatc aaaaattaca ccggcgatat
     5761 tcttaacttt gaaacagcga ccgagttact gcacgatagc ggcgtaaaag tgaccactgt
     5821 ggtcattgat gacgacgttg cggtaaaaga cagtctttat accgccgggc gacgcggcgt
     5881 tgccaacacc gtattaattg aaaaactcgt aggcgcagcg gcggagcgtg gcgactcact
     5941 ggacgcctgt gcggaactgg ggcgtaagtt gaataatcaa ggccactcaa taggtatcgc
     6001 tctcggtgcc tgtaccgttc ctgccgcggg caaaccttct tttacccttg cggataatga
     6061 gatggagttt ggcgtcggca ttcatggtga gccaggtatt gaccgccgcc ctttctcttc
     6121 ccttgatcaa accgtcgatg aaatgttcga caccctgctg gaacatggct cataccatcg
     6181 cactttgcgt ttctgggatt atcaacaagg cagctggcag gaagaaccac aaaccaaaca
     6241 accgctccag tctggcgatc gggtgattgc actggttaac aatcttggcg caactccgct
     6301 ttctgagctg tacggcgtct ataaccgcct gaccacacgt tgccagcaag cgggattgac
     6361 tatcgaacgt aatttaattg gcgcgtactg cacctcactg gatatgaccg gtttctcaat
     6421 caccttactg aaagttgatg acgaaacgct ggctctctgg gacgtcccgg tccacacccc
     6481 ggcccttaac tggggtaaat aaggagaaag caatgtcact gagcagaact caaattgtta
     6541 actggctcac tcgttgtggc gatattttca acaccgagag cgagtatctt accgggctgg
     6601 atcgcgagat tggcgacgct gaccacgggc taaatatgaa ccgaggcttt agcaaagtgg
     6661 tggaaaaact ccctgctatc gcagataaag atatcggttt cattctcaaa aataccggta
     6721 tgacgctgct ttccagcgtc ggtggtgcca gtggtccgct gttcggtacc ttctttatcc
     6781 gcgccgcaca ggcgacccag gcacggcaaa gcctgacact ggaagagctt tatcagatgt
     6841 tccgcgatgg cgcggacggc gtaatcagtc gcgggaaagc cgaacctggc gataaaacca
     6901 tgtgtgatgt gtgggtgccg gtggtggaat cgttacgtca gtccagcgag caaaatctct
     6961 ctgttccggt ggcgctcgaa gctgccagta gcatcgccga atccgcggca caaagtacga
     7021 ttacgatgca agcccgcaaa ggccgcgcca gttatctcgg tgaacgcagt attggtcacc
     7081 aggatcccgg cgcgacctcg gtgatgttta tgatgcaaat gttggcgtta gccgcaaaag
     7141 agtaaggaat tggtgatggt aaacctggtc atagtttcac atagcagccg actgggagaa
     7201 ggtgtcggtg aattagcccg tcagatgtta atgagtgata gttgtaaaat cgccattgcc
     7261 gcgggaattg acgatccaca aaatcccatt ggtaccgatg ccgtcaaagt gatggaggcc
     7321 atcgaatctg ttgctgatgc cgaccatgtg ctggtcatga tggatatggg tagcgcatta
     7381 ttgagtgctg aaactgcgct ggaattgctg gctcccgaga tcgccgcaaa agtacgtttg
     7441 tgtgctgcgc cgttggtcga aggtacactg gcagcaacgg tcagcgcggc ctcgggggcg
     7501 gatatcgaca aagttatctt tgacgctatg catgcgctgg aagccaaacg tgaacaactg
     7561 ggtttaccgt cctccgacac tgaaatctct gacacatgtc ctccgtacga tgaagaagcc
     7621 cgttctctgg cggtggtcat aaaaaaccgt aacggcctgc atgtacgtcc ggcctcccgg
     7681 ctggtttata ccttatcgac atttaatgcc gatatgttgc tggaaaaaaa cggcaaatgc
     7741 gtcacaccag agagtattaa ccagattgcg ttactacaag ttcgctataa cgatacgctg
     7801 cgcctgattg cgaaagggcc agaagctgaa gaggcactga tcgctttccg tcagctggct
     7861 gaagataact ttggtgaaac ggaggaagtc gctccaccta ctctgcgtcc cgttctgcct
     7921 gtttcgggta aagcctttta ttatcaacca gttttatgta cggtacaggc aaaatcaacc
     7981 ctgaccgcgg aagaagaaca agatcgatta cgccaggcta ttgacttcac gttattagat
     8041 ctgatgacgt taacagcgaa agcagaagcc agcgggcttg acgatattgc cgcaatcttt
     8101 tctggtcacc atacactgtt aggtgatccg gaactgctgg cggcggcaag cgaactcctt
     8161 cagcatgaac attgcacggc agaatatgcc tggcagcaag ttcttaaaga acttagccag
     8221 caataccagc aactggatga tgaatatcta caagctcgct atattgatgt ggacgatctt
     8281 ctgcatcgca ccctggtcca cctgacccaa acgaaagaag aa
//
LOCUS       DAJVQH010000149         8193 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJVQH010000149 DAJVQH010000000
VERSION     DAJVQH010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.149"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..671)
                     /gene="chbR"
                     /locus_tag="OQM87_003612"
     CDS             complement(<1..671)
                     /gene="chbR"
                     /locus_tag="OQM87_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCS1509595.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDSISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIIN"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="OQM87_003613"
                     /pseudo
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="OQM87_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="OQM87_003614"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="OQM87_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="OQM87_003615"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="OQM87_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCS1509596.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="OQM87_003616"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="OQM87_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCS1509597.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="OQM87_003617"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="OQM87_003617"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCS1509598.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINLLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="OQM87_003618"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="OQM87_003618"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCS1509599.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="OQM87_003619"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="OQM87_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCS1509600.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREISTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="OQM87_003620"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="OQM87_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCS1509601.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="OQM87_003621"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="OQM87_003621"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCS1509602.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHSEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         2152 a         2053 c         1893 g         2095 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatacta tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttaca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tgcggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttaacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctggcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatctgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattcg acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgggt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtggaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca aaacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgctg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc cctccctcgc
     8161 gctggaacgg ataaaccgaa ccgagattca gta
//
LOCUS       DAJVQH010000150         8180 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJVQH010000150 DAJVQH010000000
VERSION     DAJVQH010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.150"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..450
                     /locus_tag="OQM87_003622"
     CDS             <1..450
                     /locus_tag="OQM87_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS1509603.1"
                     /translation="NNASYQLNNALPNKETISSNFCERLEQWGNKSVNNGEERAIAVE
                     RIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNINLSV
                     NKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            560..1891
                     /locus_tag="OQM87_003623"
     CDS             560..1891
                     /locus_tag="OQM87_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCS1509604.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYIPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            2215..3603
                     /gene="narU"
                     /locus_tag="OQM87_003624"
                     /pseudo
     CDS             2215..3603
                     /gene="narU"
                     /locus_tag="OQM87_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
     gene            3685..7318
                     /gene="narZ"
                     /locus_tag="OQM87_003625"
                     /pseudo
     CDS             3685..7318
                     /gene="narZ"
                     /locus_tag="OQM87_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            7315..>8180
                     /gene="narH"
                     /locus_tag="OQM87_003626"
     CDS             7315..>8180
                     /gene="narH"
                     /locus_tag="OQM87_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1509605.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTER"
BASE COUNT         2185 a         2026 c         1907 g         2062 t
ORIGIN      
        1 aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat ctccagcaat
       61 ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga agaaagagca
      121 attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct tgatttgtca
      181 tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt aaatctggaa
      241 aacaattgtc taacttgtct tgacttagct gacattgcca gtctcgttaa tatcaacctc
      301 agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga aaacatttat
      361 attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt ggttaatctg
      421 gaagcgcaaa ataacagcct aaaaaaatga tatttctgat agttataaac tgaaatttct
      481 taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat ctctgattga
      541 gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc ccaagatgaa
      601 aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca ctacaaaact
      661 ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg atcaactaat
      721 atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc cacttgcaca
      781 aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag tgaccattga
      841 tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca cctctatcaa
      901 tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac tcgtaaagct
      961 aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa atgtaagtat
     1021 tacacatata tctatgaata ataatgctct cagaaatatt gatatagata ggctttcatc
     1081 agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag aatcttgcga
     1141 atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg caggtaataa
     1201 aaatgaactc ttactgctgg atctatccca taataaacta acaagtttac acaatgtctt
     1261 atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa ttaaaatatt
     1321 ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt tgaaatatat
     1381 aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg acaataataa
     1441 aataacccac attgatacta ataatacatc cgatattgga actttattcc ccataataaa
     1501 acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgcc ctactatgca
     1561 gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc tggcaattct
     1621 ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg aattgtttca
     1681 ctatactatt aaaaaagcat atatcccata taatatacac acttacaaga cagaggaagt
     1741 tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa acaatactta
     1801 ctataataac gattattaca atcaggcaat aagagaggaa ccgatagact ttctggacag
     1861 atcgttttcc tccaattcat ggccttttta gtgaataaat catcactaat tctataagtg
     1921 tctggtcttg ttgagaatat agttaatatg taacccttta gattcacgat ctgaagggtt
     1981 gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt tagttacatt
     2041 tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg ctttcttatc
     2101 tattctttca atcagcactt cacccaattc atccttaatt ctaataactg gctattgcgc
     2161 cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa caatatggca
     2221 ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga aaatccggcc
     2281 ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc agtcagttgt
     2341 ctacttcttg ccttctgtgt ctagatgcta tttagcgcag ttaccgttaa tctcaataaa
     2401 atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc ctccgtttct
     2461 ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg acgccaatgg
     2521 ccggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat tgccgtgcaa
     2581 aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg ttttgcaggt
     2641 gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc caaacaaggg
     2701 agcactcttg ggattaatgg cggattagga aacttaggtg taagtgtaat gcagctggtt
     2761 gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg cgtaccgcag
     2821 gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc gctactggcg
     2881 attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg cgcctcaatt
     2941 gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct gctttacctt
     3001 gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc aaaaacccag
     3061 ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttattgg tgccatcgcg
     3121 cggtcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac gttgatcaac
     3181 tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg cacaggctcc
     3241 ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgaccgc gggtctggga
     3301 agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat ttatcgggtg
     3361 aagatgaaag gcggtagtga tgagcaagct cagagagaag ccgtcaccga aacggcggca
     3421 gcgctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc gcaggcattt
     3481 ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt tttaatcttc
     3541 tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt cagccaaaaa
     3601 taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca gatcccaatt
     3661 cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg ctacttcaaa
     3721 caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa ccgcgactgg
     3781 gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac ccacggcgtt
     3841 aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt gacctgggaa
     3901 atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga acctcgcggc
     3961 tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct gaaatacccg
     4021 ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaaacaaca cagcgatccg
     4081 gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta caaacaagtg
     4141 cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct gattgccgcc
     4201 gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt ctcgccgatc
     4261 ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct gcttggcggc
     4321 acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg
     4381 ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta catcatcgct
     4441 tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac cgaagtacgc
     4501 tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc caaattgtgc
     4561 gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc aatggggcat
     4621 gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa ctactgccgc
     4681 cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg tagctacgtt
     4741 cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag caacaatccg
     4801 cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa cggttcgatt
     4861 ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc cggtacggaa
     4921 accgaattgt cgttaaccct gctcggtcaa catgatgctg ttgcaggcgt ggccttcccc
     4981 tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc ggtgctggtg
     5041 cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc agtggtcagc
     5101 gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga tgaaaacagc
     5161 gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca aattaccggc
     5221 gtaccgcgcc agtatattga aaccatcgcc cgcgaatttg ccgataccgc ccataaaacg
     5281 catgggcgct caatgattat cctcggcgca ggtgttaacc actggtatca catggacatg
     5341 aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca aagcggtggc
     5401 ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg gttgccactg
     5461 gcctttgcgc tcgactggaa ccgaccaccg cgccagatga acagcacctc gtttttctac
     5521 aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact ttcaccgctc
     5581 gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc cgaacgtatg
     5641 ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa agctgaagcc
     5701 gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc gggcgattta
     5761 cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt gtttgtctgg
     5821 cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa gtatctgctg
     5881 gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat caaaccggaa
     5941 gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt gacgctcgac
     6001 ttccgcatgt ccagtacctg cctgttctcc gatatcgtcc tgcccaccgc cacctggtat
     6061 gaaaaagacg atatgaacac ctcggatatg catccattta ttcatccgct ttctgcggcg
     6121 gtcgatccgg cctgggaatc acgcagcgac tgggaaatct acaaaggtat tgccaaagca
     6181 ttttcgcaag tgtgcgtggg ccatcttggc aaagaaaccg acgtggtatt acaaccactg
     6241 ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg gcgcaaaggc
     6301 gaatgcgatc tgatcccggg taaaaccgcg ccgaatattg tggcggtgga gcgcgactac
     6361 cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact tggcaatggc
     6421 ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa actcaattac
     6481 accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat tgacgcttca
     6541 gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa agcgtggcag
     6601 gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa agaggacgag
     6661 aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag ccccacatgg
     6721 tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt tcatgagtta
     6781 attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc gtggatgcgt
     6841 gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag cgtcagtgag
     6901 atgcgccaga taccgccaaa cggcttccag gaaaaagcac ttaacttcct gacgccgcac
     6961 cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac gctctctcgc
     7021 ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc aggaacgcat
     7081 tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata actcggtcac
     7141 ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg cctggggctt
     7201 taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc gcaagatgaa
     7261 gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga aaaaatgaaa
     7321 atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg ccatacctgt
     7381 tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc atggtttaac
     7441 aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca ggaagagtgg
     7501 caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg cagcaagatg
     7561 ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga tgattactac
     7621 gaacctttca ccttcgacta cgaacatttg catagcacac cggaaggcaa acatatccct
     7681 actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat ctggggccca
     7741 aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg caacttcgag
     7801 gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta cctgccgcgc
     7861 ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct gcccaagcgg cgctatctac
     7921 aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg ctggcgtttg
     7981 tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg caagtcagaa
     8041 aaatgtatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg ctcagaaacc
     8101 tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg catcgaggaa
     8161 gcggcgagca ccgagcgcga
//
LOCUS       DAJVQH010000151         8122 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJVQH010000151 DAJVQH010000000
VERSION     DAJVQH010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.151"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(1..953)
                     /gene="phoA"
                     /locus_tag="OQM87_003627"
                     /pseudo
     CDS             complement(1..953)
                     /gene="phoA"
                     /locus_tag="OQM87_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1054..1314)
                     /gene="iraP"
                     /locus_tag="OQM87_003628"
     CDS             complement(1054..1314)
                     /gene="iraP"
                     /locus_tag="OQM87_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCS1509606.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(1496..1699)
                     /locus_tag="OQM87_003629"
     CDS             complement(1496..1699)
                     /locus_tag="OQM87_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509607.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            1777..2871
                     /gene="ddlA"
                     /locus_tag="OQM87_003630"
     CDS             1777..2871
                     /gene="ddlA"
                     /locus_tag="OQM87_003630"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1509608.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(2895..3107)
                     /locus_tag="OQM87_003631"
     CDS             complement(2895..3107)
                     /locus_tag="OQM87_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCS1509609.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            3367..3675
                     /locus_tag="OQM87_003632"
     CDS             3367..3675
                     /locus_tag="OQM87_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCS1509610.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(3734..4828)
                     /gene="yaiW"
                     /locus_tag="OQM87_003633"
     CDS             complement(3734..4828)
                     /gene="yaiW"
                     /locus_tag="OQM87_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCS1509611.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(4841..6061)
                     /gene="sbmA"
                     /locus_tag="OQM87_003634"
     CDS             complement(4841..6061)
                     /gene="sbmA"
                     /locus_tag="OQM87_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCS1509612.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            6413..7570
                     /gene="ampH"
                     /locus_tag="OQM87_003635"
     CDS             6413..7570
                     /gene="ampH"
                     /locus_tag="OQM87_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCS1509613.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(7571..8074)
                     /locus_tag="OQM87_003636"
     CDS             complement(7571..8074)
                     /locus_tag="OQM87_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1509614.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2039 a         2072 c         1932 g         2079 t
ORIGIN      
        1 tacactgtca ttacgttgcg gattaggcgt ataggtgact gcgggcttat cgatattgcc
       61 gtggtacgtt gctttcggcc ctagccagcg cactggcata ttgccgtcag caaacaggcc
      121 aagcaggggt ttttgctgat tcgcttccgt caccgaattc agtgaggcag catcgttcac
      181 caactgataa ccacgcgcct gtgcctgttc acgcagcgtt tttccctgcc attcaccagc
      241 ggttgccgtt tcagcaaagg tttttgcgcc gccgccaagc gtaacgtcgg cacgagcgtt
      301 aagcagctgt tcggtaatcg atccttttcc gcctttttcc agagcgttac ccggacattt
      361 ttcactggtc gcgctcggac cgtagcattt gcgcgaagtc acatgtgcca ccagcgcagc
      421 gggcgtggca tcctgcaact ctgcggtaga aacgttaccg gtcgccagac ctgcggcttt
      481 tgccatttcc agaatcgttg ggtgatcttt ttcgtgaata tcgacgccca gcgcgccgtt
      541 ataggttttg acaccggttg accaggcggt tgctgatgca gccgagtcgg tgacgtagtc
      601 cggtttgcca gttttttatt cagcgcatag tgagtgtatt gcccggtaag cggtaaggca
      661 tcgatacctt taaaaaagcc gcccgcacct tcggcataat tacgtgcggc agtaatttcc
      721 gaatccccca tcccatcgcc aatcagcaaa ataatatttt ttgcaggttt atcgctaaga
      781 gaatcacgca gagcggcggt ctgatcaccc gttaagcggc gagcaccgcc gggtgcagta
      841 atatcgccct gagcagcccg gttttccaga acaggcattt ctggtgtccg ggcttttgtc
      901 acaggggtaa acagtaacgg taagagtgcc agtgcaatag tgctttgttt cactttattt
      961 tctccatgta caaatacatt aaaaaataaa aacaaagcga ctataagtct cggccgtgac
     1021 aactttatga cagctattga aaagattaac tttttactga cgaggatgct tcaataactt
     1081 ctttacgtaa tcgcgcagca gctccgtatc gtcgtcagga atgctggcat cgggctttac
     1141 ctcgtacagc gccccctcta cctgatcaat caaccgctgt tggtcatttt gcgccatatt
     1201 gcgaagcatt gcagtgacga taatctccaa agcttctacc tgcgcacaca gttctttcga
     1261 ctcttcttct ttttgggcaa gcttaaataa caactcagta atgagatttt tcatgtctgt
     1321 atttccttat ccaaagtatg gagaagttaa cattatgttt cacaacttca tagtgtctgg
     1381 aagtattatt tttatgacaa aggaaatact ttgcgcaaat attttttgtt tgattaccag
     1441 cgaaacgttt ctcttattta aataattacc cacacaaaat ataggctttt aattatcaac
     1501 ctttattata aataaaagct atgaaatata ttctcttcgt taacttataa ttaagagaaa
     1561 aaaactcacc gcattgccat ttaagccagg aataaattag cttcccgttc atcatttttg
     1621 tgatcttgcg cacatctttg agataacgca ctttcttcac cagtacgttg cacgctttgt
     1681 cagcagaaga ttgcaacaat ctttcagaag gccgcgaatc ccccgttttg tcgtggtaat
     1741 gtatgcactt tgtgttagac gggataggtt tttaagatgg aaaaactgcg ggtaggaatc
     1801 gtttttggtg gtaaatcagc ggaacatgaa gtgtctctgc aatcggcaaa aaacattgtc
     1861 gatgccattg ataaaagtcg cttcgacgtt gtgctgctgg gcattgataa acaagggcaa
     1921 tggcacgtca gcgatgccag caattatctg ctaaatgcag acgatcctgc ccatattgcg
     1981 ttgcgccctt cggcgaccag ccttgcgcag gtgccaggta aacatgagca tcagcttatc
     2041 gacgcgcaaa acggtcagcc gttgccgacg gtggatgtca ttttcccgat tgtccacggt
     2101 acgctgggcg aagatggttc cttgcaggga atgctgcggg tcgccaattt accgtttgta
     2161 ggttctgatg ttctggcttc agcaacctgt atggataaag atgtcaccaa acgtctgcta
     2221 cgcgatgccg ggctgaacat tgcgccattt attaccctga cgcgcgctaa tcgtcacaac
     2281 atcagttttg ccgaagtgga gtctaaactg gggttaccac tgtttgtaaa accggctaat
     2341 cagggctctt ctgttggtgt cagcaaagta accagtgaag aacagtacgc aattgccgtc
     2401 gatctggcgt tcgagttcga tcataaagtg atcgttgagc aagggatcaa aggtcgtgag
     2461 atcgaatgcg cagttctggg caacgacaat ccgcaagcca gcacctgtgg cgagctcgta
     2521 ctcaccagcg atttctatgc ctacgacacc aagtacattg acgaagatgg cgcgaaagtg
     2581 gtagttccgg cagccattgc accagagatc aacgataaga tccgggcgat tgccgttcag
     2641 gcttatcaga cgttgggatg cgcaggcatg gcgcgtgtag acgtgttttt aaccccagag
     2701 aacgaagtgg tgatcaacga gatcaacaca ctgcctggct tcaccaacat cagtatgtat
     2761 ccgaagctgt ggcaagccag cggtctgggt tacaccgatc tgatcacacg tttgattgag
     2821 ctggcgctgg agcgtcacgc tgcggataac gcattgaaaa ccacaatgta atattttaac
     2881 gcccgataat attcttattg ggcgttttca gtttcttcat cgcgacgacg aataatcaac
     2941 cccgccagcc agaaactaat cacccacgtc accagaccga cggcataggt ctgccaacct
     3001 tttgcttcaa atcccagtac ccccaccacg ccattaagaa tgaatataag gccaatggcg
     3061 aacgcatagt agtgccagtc acggcggatt tttacaggca gattcatggc agctccgtta
     3121 acgaaaaaca acatccttgc atattctgta acaaccgtct gtggtgtaga caacattttc
     3181 gtaattgtta ataaatttaa cttaaggttg ccattttgtg actcacaacg aagattcacg
     3241 cttcgggaat attcccaaca tcaatttacc atgattctga tattgacaat ctaaatgacc
     3301 tgtcagactt gccataagcc gctggtatca caccagaaga gaagcagatg acttccggag
     3361 gtttttatgg ctgatttcac cctgtcaaaa tcgctgttta gcggaaaata tcgcaatgcc
     3421 tcttcaacgc ctggcaacat tgcctatgcg ttgtttgtgc tgttttgctt ttgggctggg
     3481 gcgcaattgc tgaacctgtt agtgcatgcg cccggcgtct atgagcgttt aatgcaggtc
     3541 caggaaacag gtcgcccacg ggtggaaatt ggtttaggtg tcggcaccat tttcgggctg
     3601 atcccgtttt tagcaggctg cctcattttt gcagtggtgg cgctatggct gcactggcga
     3661 catcgccgcc agtaaaattt acgttgccag atgcggcctg aaacgcctta tctggcctga
     3721 cattcctgtt tgatcactgt ttcatacaac gcgcccgccg ttcgtctacg cgcttcgcaa
     3781 accaggccgt agtcaggttg cgggtgattt tcgggctttc cagttgaatg ccaggcaaca
     3841 tttcgcgggg tagtgattta cccgttttcg cttcggcaag ttgataaact ttcttgtaca
     3901 gcgccgtctc ctcaaaagag aagctgtcac ctttctctaa ctgacggcga atttcgctgt
     3961 cgctcatccc cagttttccc gccagtttgc gcgttgccag ttcggttttc ccgggttctt
     4021 tactgtcgta acgaattaaa tcaccgtcca gtgccagctt cacgccgctg gccttactga
     4081 ctgcgttctg aaacgcagca ttacggctgg cgtaccagcc agcgttaaaa tcagcaaagc
     4141 ggtatatcgg tgcgctatag ctggcgggat aattcagtaa atggtaagaa ccaaaccaca
     4201 acccgccgcg gcgactgaag acttcctgac gaactgtacc gtccattttc cacggatacc
     4261 ctttggtatg ctgttcggca aaagcaatgc tgacctgcat cggcccaccg gtgcgcaccg
     4321 gattaagcga accaaacagc gtctgcccca ttggtaccat gctgatcaga tcgtcaaaaa
     4381 tcgcgctcaa ttgcttttct gtacgcaccg aatctaaccg ttcgctataa cttttgccgt
     4441 ttggcgattt gatttttagc gcggtatgaa ccagaaaagc aggaatgtgc atccgttcag
     4501 cacgacggtc aatctcttgc caggcgatct tgcttaagcc cggaacggcc ggatctgcct
     4561 gataatttga ctcctgctgc gccaccgcca gcaccgagca gacgttttcc agcgtcggtg
     4621 ccagcccctg actttcaaag gtggtggcga gatctttcgc ccaggcatcg cggtctttca
     4681 cgctcgcagg catcttctgc cgcacgacgc tcgctacatc tatcgctttt tcgccctttt
     4741 tcaacggttg cggagcctga ctactacacc ccgctaaaac cagaaccgcc aacaatgaca
     4801 gcgaagatag atgattgacg cgcgacatta cttctccttt ttagctcaag gtatgggtta
     4861 cttcctgaat tttgtcacca tccagctcat gttcaaagct gcgcagacgt ttgtagatag
     4921 acatcaactc aaccagtgtg gtccatgagt taatcaggta ctggaaagca ccgcgaacct
     4981 gaccaaaaac gttggtaatc tgcgtcatca ggccgagcgt aatcgtaccg gcaacaattg
     5041 acggaaacag caagaacaaa ccgaaaacgt tatcgacctg caaatagaga atgcgggcga
     5101 tgttgaaata catatagtga aaatagaggc gaaaatagtt tttccgtacg gcgctaaaca
     5161 gctcgcgtac cgtaggcggc gtcgcgcgcg tggcatcgtc ttcaccataa accagctctt
     5221 tacggtaggc ggcctctact cgctggtttt taaactccag ccccggcagt ttgatcccta
     5281 ccactgccag caatccggtc cccatcagcg accagacgat agcggcaatc accagaccat
     5341 acggaatgtg cccgataatc ggcagctccg gcacatgcgc ggagagcgtt accagcaccg
     5401 gcaggaaggc gatcaacgtc atgatggcgt tgataaaact gacgcccata ttctccagcg
     5461 ttgaagcaaa acgcatggtg tcttcctgca cacgctgtgc ggcaccttcg atatgacgca
     5521 gttgttgcca gttcgccatg taatattcgt tcatcgccgt acgccagcgg aacacgtagt
     5581 gactgacaaa gaagttgttc agtacactga tcaccaccgc gatcagcgca atccccagaa
     5641 agacgcccac ttcgcggtaa aattgttcga tagtgacttt atgcggcgaa cttagcgcgg
     5701 tttgaatcag atcatagaac ggcgcatacc aggcgttgac ggcgaccccg acttccacca
     5761 aaaaccaagt gacgaagata atcagcgcag taccgagtat tgaccagtat tgccaacggt
     5821 gcgggctgta gataaaccag aaaaatgcaa aaagtcctac gcaaacaatg tagtaagcgt
     5881 aaaaaatcag gaaatccaac gaccagaaac gcgcggcgct aatcgggatc tgcccggaag
     5941 cgccggtgat acgtgccacc cagtcacccc caccggcttg ccagaagata acggcaatca
     6001 atgcccaaac aaaggccgag agaaaaaacg ttcccggctt tgggaaaaaa gacttaaaca
     6061 tatgcactcc tgctaacttc ttatcgtttt aacaactgct gtgtattaac cgcatgaccg
     6121 ctacaaagcg tcagccaaat gaaaccgccc attgtgaccc atagcaaggc gcttagttcg
     6181 ccagcctgac cataagaatt gtttcgacgg atttcacaaa ccaaagattg tttacataag
     6241 cggattaaca cgcgcaggat cacaaatgca ggcagtggtg agcaggaaaa agatcgtgtt
     6301 tacgcgccgt tacgttttta acactattat tgcactaatt cgccctttgc aatccatcaa
     6361 tgagtagtat aaatacgctc agttaccttc attcaatcta tggacaccac cgttgaaacg
     6421 tagtctgctt ttttctgccg tgctgtgtgc ggcgtcattg acctccgtcc atgcggcgca
     6481 gccgatcact gaaccggagt ttgcctctga tattgtcgat cgttatgccg atcatatttt
     6541 ttacggcagc ggtgccacgg ggatggcgct ggttgttatc gacggtaacc agcgcgtctt
     6601 tcgtagttat ggcgaaacgc gacctggtaa taacgttcgc ccgcagctgg attcggtcgt
     6661 gcgtattgct tccctcacca aactaatgac cagtgaaatg ctggtgaaat tgctcgacca
     6721 ggggaccgta aagttaaacg atccgctaag taaatatgct ccgccaggcg cacgcgtgcc
     6781 gacctacaat gggacgccca ttaccctagt gaatctggca acccatacca gcgccctgcc
     6841 ccgtgaacag cccggtggcg cggcacatcg tccggtgttt gtctggccaa cgcgcgagca
     6901 acgctggaaa tacctttcta cggcgaagct gaaagccgcg ccaggcagtc aggcggcgta
     6961 ctctaacctt gcgttcgacc tactggcaga tgcactggcg aatgcgtccg gcaagcctta
     7021 tacccagttg tttgaagagc agattaccca tccattgggg atgaaagaca ccacctacac
     7081 cccctcaccg gatcagtgcc gccgtttaat ggttgctgag cgcggtgcca gtccgtgcaa
     7141 taacacgctg gcggcaattg gcagcggcgg cgtctattcc acgcctggcg atatgatgcg
     7201 ctggatgcag cagtatttgt catctgattt ttatcagcgc agtaaccagg cagatcgcat
     7261 gcagacgttg atttatcagc gcgcgcagtt tacgaaagtg attggcatgg atgtccccgg
     7321 caaagcggat gcgcttggtt taggctgggt atatatggcc ccgaaagagg gtcgtccgag
     7381 gattattcag aagacaggcg gtggtggcgg tttcattacc tatatggcga tgatcccgca
     7441 gaagaatatt ggcgcgtttg tggtggtcac tcgttcgccg ttgacgcgct ttaaaaatat
     7501 gagcgatggc atcaatgatc tggtgaccga gctaagcggg aataaaccgt tggttatccc
     7561 cgcatcctga ttaatactct gaaggcaaac ggttgatagc gaccagtttg cctttattca
     7621 tttcgatata gccgccttta cgcaaagcag cgagaacttc tgcgacgaca gaacgcgata
     7681 tgcgtgtacg ttgatggata taattcatca caccaatacg tgatcgcaat tcttcattcc
     7741 agtcaatcat tgataataat gttgcgcggg tttgtttggc tggtagatga tatcccgtac
     7801 aatttccttc tgatattaat ttgtatggta tatcgttttt cattaaacca tcagccagcc
     7861 ccataatata aggagcctgg gtaataccga taagtacgtt ttcttctcta cgcagagaga
     7921 taactccttc taaaataacg aaagtatctt cgttattgac atcactggaa aatataactt
     7981 gcttttcatt attaaactcg aagcgcgtac cgtatctgga caaacattta tcgagcttac
     8041 caaattcctg aagaggttta actacagata acatttgcgc gtcctttgca gtaatgcccg
     8101 tcaaatcctt gacgggcatt at
//
LOCUS       DAJVQH010000152         8122 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJVQH010000152 DAJVQH010000000
VERSION     DAJVQH010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.152"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(8..883)
                     /locus_tag="OQM87_003637"
     CDS             complement(8..883)
                     /locus_tag="OQM87_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCS1509615.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPACMSRH"
     gene            943..1221
                     /locus_tag="OQM87_003638"
     CDS             943..1221
                     /locus_tag="OQM87_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509616.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1222..1749)
                     /gene="gadE"
                     /locus_tag="OQM87_003639"
     CDS             complement(1222..1749)
                     /gene="gadE"
                     /locus_tag="OQM87_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCS1509617.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2548..3120)
                     /gene="hdeD"
                     /locus_tag="OQM87_003640"
     CDS             complement(2548..3120)
                     /gene="hdeD"
                     /locus_tag="OQM87_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCS1509618.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3375..3707
                     /gene="hdeA"
                     /locus_tag="OQM87_003641"
     CDS             3375..3707
                     /gene="hdeA"
                     /locus_tag="OQM87_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCS1509619.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3823..4149
                     /gene="hdeB"
                     /locus_tag="OQM87_003642"
     CDS             3823..4149
                     /gene="hdeB"
                     /locus_tag="OQM87_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCS1509620.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4213..4859
                     /locus_tag="OQM87_003643"
                     /pseudo
     CDS             4213..4859
                     /locus_tag="OQM87_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4901..5431)
                     /locus_tag="OQM87_003644"
     CDS             complement(4901..5431)
                     /locus_tag="OQM87_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1509621.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5587..6153)
                     /gene="slp"
                     /locus_tag="OQM87_003645"
     CDS             complement(5587..6153)
                     /gene="slp"
                     /locus_tag="OQM87_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCS1509622.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
     gene            complement(6400..7623)
                     /locus_tag="OQM87_003646"
     CDS             complement(6400..7623)
                     /locus_tag="OQM87_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509623.1"
                     /translation="MSIDFTLGIINTYHGDIYNCTTNTDNVKTPDTPKWPYDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         2361 a         1569 c         1699 g         2493 t
ORIGIN      
        1 tcatccacta atgccgtgac atacatgcag gcagcaagtc accatttggg ttggggaaaa
       61 tcgcccgtaa cgtcacggag cccgtggttt catcaaccgt cgggtcggag aatttcagcg
      121 tgccggtctg gctgtaacgt ttaccatttt ccagattgag ctgtactggc gtactgccct
      181 gaacctgttt gatttgccca ctggcgacct cttctttcat gcgtaagaaa tcttgcaccg
      241 actgcgtgag atcgacataa atcgggtcca gacgttgtac ggtaaccagc gaatctgcct
      301 gattagcggt aacgagtgcg ccgacggtca ccgacgattt cccgctgacg cccgtaatcg
      361 gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg ttcaacagcc gctttggcga
      421 cggtgacatt ggcttctgct tcattcagct gggtgcgcgc ggtgtcgtaa tcctgacggc
      481 taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg gcattgctgg
      541 cggtagagag cgctttcgcc agcgagcctt tggcggagtt tagctcggcc tgtaaaggtg
      601 caggatcaat ctgatacagc gaatcgctct ggttcacttt atcgccttcg ataaagttgc
      661 gtttaatgat aataccgccc acctggggac gtatctcggc agcttcataa ggaacggttc
      721 taccgggcaa ttcgctcaac acattgaccg aactagggga gagtgtgacg acaccgacct
      781 caggcgtcat ggcggcggcg ttttccgccg atttgtcatc gcaggcggtg agcatcgcgc
      841 cgcagaataa caacggtatt aacagctttc ttcttctgtt cattttagtc cctgaaaatt
      901 cttgagaaat aacggttctt taaattgcgc gcgaggagag atatgaggga tttacagacc
      961 agcggcatag tggggttgtc agccagtaag gtgggataca ggcacagtga tcgacatggt
     1021 gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag ttcctgccag cattcgggca
     1081 acgaagggtt ctactggtgg atacacatac caggggaatc aagtcgtttt agcctctcct
     1141 ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc ctcgtcatgc cagccatcaa
     1201 tttcagttgc ttatgtcctg actaaaaata agatgtgata cccagggtga cgatgtcgct
     1261 catacgtctt agtttcagct tgtacataat gttgaatttc tgaatcttta atgtttgttg
     1321 cgaaatattg agcgtcgacg tgatattgct ttcattttta tttttcagaa tcagttgcat
     1381 aattttgaac tgattatgtg aaaaacataa ttgtgaattc ttatggggca agtgtttacc
     1441 ataagtaatc tcaagaagat ttttgatact ttctttgcgg ctaacaaaat aaataggcgc
     1501 tttagctttt agtttactga tgtgataagg tgccaaaaca ataattctct cggcatctaa
     1561 tttctccaga aatttaatcg cttcttcatc aaggatatga ttgtgatagg tatcaatgat
     1621 aaccttgaag ggtttattgc ctactttttt gatctctgac agggaattga tttttatcat
     1681 ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt tcgtcatgag
     1741 aaaaatcata acttgctcct tagccgttat tgtttcgaac atgtcatcct tttcattatt
     1801 tacatccttg tccgaatcgt tgttcaatat agtaaacgcc tataagaact ttgcccttcg
     1861 tgaactaata cgtctattaa cactaatcaa tacttcagat ttatccatca cgcactccat
     1921 atattgatcc taagtaagat tcctggttgt tatcagcttg taaatagtaa acaaaaagtt
     1981 aacgtttgcg gattactgcc caagaataag ttatcaataa ccagtttgtg atctctgaag
     2041 aatattacta aagttaaaat gtaatccgat ttaaatatcg agtctccttg tttcgactta
     2101 agctggcaat tggattgcca gctttctttt aacgcattga aattacctga gtaaataacg
     2161 catttatgtc ttcaggtttt tatatagcgt ctggagacac ggatatttat gcaatgtaat
     2221 caacgccagt tgcaaaaaaa tgaacccgga ttacttattt aaccgcccat agcatcaacg
     2281 cttacggaac tctttgttca ctttcccaca gcacttttaa aacattaaac ctgactacgg
     2341 aaaatatcag ccatgataat gtttgaaatg gctaatttgc catggagtga aaaaaattag
     2401 atgaaattca gtaggttgaa ataatcacta gcaggtaatt atttcaatga tagtgcgcaa
     2461 ttgatctaca acactgcgta gcggagagag tattaatcgg atcatagtca catcaagtga
     2521 ctatgatccg ggtgacaacc ggggtaatta ttgctgctta acgaacaaac tggcgaagct
     2581 gaacaggctg gcggcgctaa atatcagttc aattcccacc agtgtggaaa ccagcgttac
     2641 agacaccatc ggcgttgcac caaggaatat ccaggcaatg acgatatcca gcacaccaat
     2701 aacgagctgt agccagctgc ctttcattga acgctgacga taccaactca tcaggcgaat
     2761 aacccctgca acacagaaca aaccggcaat aaatgccgca atggcaaaaa tgcccagctc
     2821 cggtgcacgg atgaagaaat agccgatcaa taaataggcg actgcgacga ggaaaccgga
     2881 taataccggc cagaaattat gactgcggtt gctgaataac ccgacaataa gcgcaatacc
     2941 cgagcagatt aataatgcac ccactactgt gcttaaaata tcgccagaga cgaacgggaa
     3001 actgatacac agcaacccga cgataaacag cagcacggca ataaactgga ttgctctgcg
     3061 atgctttttc agcatctcca gatcaaactt caaaattgtt gccttatcta tatataacat
     3121 agaaccaccc tataaaatta agaagaaaat cccctgctat caatctatgc caaaaacgcg
     3181 tctaagaatg cagtcgattt aataaaaatt tcctaattgc agtatctgat gcatctgtaa
     3241 ctcattgtat tgaaataaaa atatctgatt ttgatatttt ccatcaacat gacatataca
     3301 gaaaaccagg ttataacctc agtgtcgaaa ttgattcgtg acggctcttt cactttatag
     3361 ttgaggatat tacgatgaaa aaagtattag gcgttattct tggtggtctg cttcttctgc
     3421 cagttgtgag caatgcagcg gatgcgcaaa aagcagctga taacaaaaaa ccggtcaact
     3481 catggacctg tgaagatttc ctggctgtgg acgaatcctt ccagccaact gcagttggtt
     3541 ttgctgaagc gctgaacaac aaagataaac cagaagatgc ggttttagat gttcagggta
     3601 ttgcaaccgt aaccccagct atcgttcagg cttgtactca ggataaacaa gccaacttta
     3661 aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga tatgtaattc cgggaatgcg
     3721 ttacatcgta cttccttgca tattgaacag gccggaatat cttctttaaa agcagctatt
     3781 cctcctgttc atatataatc tctatattga atgggttaca aaatgaatat ttcatctctc
     3841 cgtaaagcgt ttatttttat gggcgctgta gcggctttgt cactggtgaa cgcacaatct
     3901 gcgctggcag ccaatgaatc cgctaaagat atgacctgcc aggaatttat tgatctgaat
     3961 ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg aagaaacagt atataaaggt
     4021 ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa ttcctaaagt gatcgaatac
     4081 tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa atcaagcatc taatgacttg
     4141 ccgaattaat gaggtacaag taaaaaggag tagcaagttg agccatcttg ctgctccttt
     4201 ttgcattttt atatgacagc agaattaatt atacgtctta tactcgcggc aattgcctgt
     4261 ggcgctattg gcatggaaag gcaaatgcgc ggcaaaggag cagggttacg cacacatgta
     4321 ttaattggca tgggaagcgc cctgtttatg attgtttcga aatatggttt tgctgacgtg
     4381 ctgtctttag atcacgtcgg actcgacccc agccgtattg ctgctcaggt ggtgacgggc
     4441 gtcgggttta tcggtgcagg taacattctc gttcgtaacc aaaatattgt aggtctgacg
     4501 acggcagcgg atatctgggt gaccgccgca taggtatggt tattggcagc ggtatgtacg
     4561 aactgggtat ttatggttca gtgatgacct tgttggtgct ggaagtcttc catcaattaa
     4621 ccttccgcct gatgaacaaa aattaccatt tacagctgac attagtgaac ggtaatacgg
     4681 tatcaatgct cgactggttt aagcagcaaa aaataaaaac tgatttagtt tcgttgcagg
     4741 aaaatgaaga tcatgaagtg gtcgccattg atatccagct tcatgccaca acgttggtag
     4801 aagacctgtt gcgattatta aaaggtatgg caggggtgaa aggcgtttca attagctaat
     4861 ctgtgtggca gcgtagccag actcaccgta agcctgaaat tcacaccaga taatcaatat
     4921 gctgatggcg taccagctca ttgatccgtt taagattgag gatcatcatg atattgtgtt
     4981 tatggcagta gaacgttttt agcgatattt tcagtttctc cagaatatct tcagttgtca
     5041 tttgattcgc aatgcagctc agaacctgat gttgcttgcg cgaaagtgac acattcgctt
     5101 caggcacaga gcagtaactg ccattgatga gtgcgacaaa atcaatgata gtgatagtcc
     5161 gcggaacaaa ggtcaccggt actttcccca gacagcgttt aatattaaag ggggagaaaa
     5221 caatgacatg gacaggtttt aatcgttcaa tttgagtgag aaaattagaa tgaaatacat
     5281 tattcattaa tgtatcaata atgacgaagg cattctgatg caacattacg tcgatctctt
     5341 cttcgttctg tatatgaacg acattacctt tactcagaat gtttttcatc gcggtgaaga
     5401 acatcgtatc cctggtaatt ataagaaaca taaccgactc ctggtgcgga cattaacttt
     5461 ataatcagtt tgccaaaggt aattaacagg acagggctta taataataat agttagctta
     5521 ctgtatatta attttatgta atacatgatg cagatgctga gaaagcatct gcatctttcg
     5581 gtggtgttat ttgaccagct caggtgttac ctgactcacc gcattggtgt agtaaggcgc
     5641 acccaagccc ggttccggcc agaatgcacc atagccgtaa tcccacaggt tataggtggt
     5701 attaaccact tctctcaaat gccacacctg gatgccctgc atattcactt ccaggaagtt
     5761 atacgggact ttattgataa agcctggttg ttcaccctga atggtaccga ggatggtaac
     5821 aaagtgatta cgatagttca ctggatcaag gaagccgctt tgtctggcga gcagtcggcc
     5881 ctgatagttg gcttcaatat caggcttcgc atagctatcc aacggtaata cagcgatttc
     5941 taacaacgta tccgttttgc cattgataac gttgataacc ttcccaccaa agcgcgcttg
     6001 ttgaccaaca tataaccccg gctggttatg aacagcaaca aaactttttt gaatatcagg
     6061 ttggttattg cctttgatat tttgcggaat tgaactacat gcggcaagca aaaatgaaag
     6121 gctgaggatg agtgcacctt ttgtcatgtt catgttacta tccttatcaa caaatattaa
     6181 aaccataata gatatttctt tatagttttc atctgattct gaggtgatat gcctgatagt
     6241 aacgtaaacc tgtctgatgt ttatatccct tctgtttagt tcaattccgt tattttgggg
     6301 cgtggtatga gtatccatcg cccaataata taaccctttg ttttaaaatt acataaaatc
     6361 tatttttatt caatgattat aaagtgctaa taatcaatat tatggtatta tattctgata
     6421 atgatttgat aagcgaattc ttttgccaga tggtatttct atttccaccc cataatttat
     6481 atctttttca ttaggcagat ctctctttaa ataaaagggg tatgaaatct tttcgggttg
     6541 aagtgtctcg taatgaaatg ctatgaattc atttttagac attgttgccc atttttgaat
     6601 tttattgtat agagagggtg ttgtaaactc ttgaagttgt ttataacata tttttttgaa
     6661 ctcttgctta tattttaata tgtcatcaaa tgaaagattc actacctttg cctctaagaa
     6721 atcaattatt ctcgaagcaa tttccgcttt cgcgcgtaaa gagttgtcat taatagttaa
     6781 caatgtagaa aacaagccat tgatttgttt gtcaagatgt tgttcatatt tatgtagttt
     6841 ggggatggga ttttccttgt gtgaaaaaat agtgttaaat aaatttgcgt cagtgaaatt
     6901 tatattaagt agatcttcaa aataaaattt tgataaggtt aggactagct tggacatatc
     6961 ttgccctgtc atgttgaaat caataaagtc gcatccagca agggcttcca tcctattttc
     7021 atttttactg tgctcaatat acagcttcgc catttttaag tacaagcttt gaatgtctct
     7081 tatatcggtt ggtaagtcag ctcgttgccc agcaaaattt ttattatcgg aagaaacggt
     7141 ttggacactg aatctttcgg gtattccttc ttctacgaga attttagtta tgattggatt
     7201 gttgggttct ggattaacga caacaatgat ttgacataca gaatccttat ccttttcgta
     7261 tctaatgtta tgaatctgat taagtattga ttgtgtctgt tgcgtaagtg gtgtcgaact
     7321 tatcgtttca atgttattaa taagacttac aagttgaacc acaacttttg agtagtctgc
     7381 atttgtatat gtggtgttcg tgtggcatgc attaatttgt tggagttgtt gttgcgctaa
     7441 atcaaattgt tcagggttgt atccttctcc agagaatgtg gaattaatgg gttgttgttc
     7501 ttcccaatta tcataaggcc acttcggtgt gtctggcgtt ttaacattat cggtatttgt
     7561 tgtgcagtta taaatatcgc cgtgatatgt atttattata cctagggtaa agtcaattga
     7621 cataacattc cttattcatc atctgtttta tgctaatgtt tttaaattgg ataattagaa
     7681 caggaatgga ttttcttcta cacacaataa atataatgcg ttatatcttc ctgatataat
     7741 tacctttaaa ttccctttca tttccacccg gtaatatttg tgtataaaaa aagggcagtg
     7801 atagtatcgt aaaggaaaac gttaacaact atcaaaaagc gctcatttta aaaagttatt
     7861 atgttctccg atagtgtgtg aagtaatatt tttgtgatgt tttagatgtg taataatgaa
     7921 ccaggttaaa gtgtatttaa aaatgtgttg tattagcgac tgaaatctgc cttttaaaaa
     7981 ggaggagggt actgagacaa agagtcattg cttctccgat aaaatgtcag agaagcatga
     8041 ttacgtggtt attgtctgtt gatgacttgt tgaccgagcc gtcatcattc tcaatgcatt
     8101 agttaaatca tttgtgattt tt
//
LOCUS       DAJVQH010000153         8069 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJVQH010000153 DAJVQH010000000
VERSION     DAJVQH010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.153"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..1232)
                     /locus_tag="OQM87_003647"
     CDS             complement(<1..1232)
                     /locus_tag="OQM87_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1509624.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRNAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQR"
     gene            complement(1229..2107)
                     /locus_tag="OQM87_003648"
     CDS             complement(1229..2107)
                     /locus_tag="OQM87_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1509625.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(2118..3025)
                     /locus_tag="OQM87_003649"
                     /pseudo
     CDS             complement(2118..3025)
                     /locus_tag="OQM87_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3012..3245)
                     /locus_tag="OQM87_003650"
     CDS             complement(3012..3245)
                     /locus_tag="OQM87_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509626.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(3242..4099)
                     /locus_tag="OQM87_003651"
     CDS             complement(3242..4099)
                     /locus_tag="OQM87_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1509627.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            4124..4453
                     /locus_tag="OQM87_003652"
     CDS             4124..4453
                     /locus_tag="OQM87_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1509628.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4443..4751
                     /locus_tag="OQM87_003653"
     CDS             4443..4751
                     /locus_tag="OQM87_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1509629.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4793..4969)
                     /locus_tag="OQM87_003654"
     CDS             complement(4793..4969)
                     /locus_tag="OQM87_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HCS1509630.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4966..5376)
                     /locus_tag="OQM87_003655"
     CDS             complement(4966..5376)
                     /locus_tag="OQM87_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCS1509631.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(5373..5651)
                     /locus_tag="OQM87_003656"
     CDS             complement(5373..5651)
                     /locus_tag="OQM87_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509632.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQAPRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(5726..>8069)
                     /locus_tag="OQM87_003657"
     CDS             complement(5726..>8069)
                     /locus_tag="OQM87_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCS1509633.1"
                     /translation="GDCEPCLFGWQALDAGVRAVVLCEGEIDCMSYAQYGISALSVPF
                     GGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASRLGEHRCRLVTLPYK
                     DINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWES
                     LADKFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPS
                     TVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSE
                     TKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAE
                     LPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTRIG
                     MLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRTRVKTGVTYTYKDDK
                     RHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRNVEKIGNAECLCIMV
                     DHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKPGILLKRLTRQATCC
                     KMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRYGIQLFIIDSLMKCG
                     IGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTGKMDVKGSGAITDLT
                     DNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEKQRNGEGWEGGVPLF
                     LDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         1917 a         2198 c         1849 g         2105 t
ORIGIN      
        1 ttacgttgtt tcgtgatatt gatttctgcg atgcccctgg cctgcgtatc cgggttgtac
       61 acttcatccc tgtaaagcat cagaatgatg tctgcatccg cctctatttc tccggaattt
      121 ttcaggtctg agttcatggg acgtttattg ggtctggact ccacaccgcg ggagagctgg
      181 ctcagcgcaa tcaacggaaa accaccggat tttgccaggc ctttaagccc ctttgagatt
      241 tcacccacgg caaggtcatg acgccccgtg gttcgggttt ttatcagccc gagataatca
      301 accaccacca gcgccgtttc cggatattta atcaggtggt gtttcgttgt tgcgcatatc
      361 tcatcaatgg ccaggttcgc ctggtccacc atccagatat tgcgcccggt catccgcccc
      421 accccttgtg agaaacgcgt ccagtcttca tcttcaaagt gagccacaga tttcaggcgt
      481 gatactggca tccctccagc cgcagacacc atacgttcac caatctggat gttcgccatc
      541 tccatggtga acagaagcac accatgcccc tgctcagtca ccttgtcgat gatgtccagc
      601 gcaagttcgg ttttccccat cgaaggacgg gcggcaatga ataccaggtc tccgggctcc
      661 ataccgcctg tttttgcgtc cagttcatca ataccggtca tcaacgtcct ggatttctcc
      721 agcccctgat tccggcattc aacacgctca accacttccg gaagcacatc atcaatatgt
      781 accggctgaa tgacgccctt tcctgtcgac agtgtgacca tcatgttctg cgcatccttc
      841 agggcatctt cagctgcttc acaggtatgc gcatcacgta atttctgcag cgcctcattc
      901 agtgtttttt ctgcattgcg cagtgcggca ttgcgccgca acgctgcaac atagtgctcc
      961 agtgaagact tcacccaggt tttacgcccg gtatcagtaa tcaccggggc aagttccggc
     1021 atctcattac acaacagcac ggggtcaatc actcctgaaa cacgggcctg tctgcagatg
     1081 cctgtataga tatcctgata cgctcgtaca gaaaaaacgt ccgctggcag tgtggccaga
     1141 atatccatca cttcaggatc tgctccgcgc agaaagaacg cgccgatgac agcgccttca
     1201 aggtcatcgt tacgccatac tggagttgtc atgcagccac acctctgata cgagaacggt
     1261 aactgggcca gttaaacgac aaccagttgc gtcccccgtc tgtgatcctg tcggcaattc
     1321 ggggactgat gaacgcccac aactcttccg gtgagaggtt actgatcaga atggtgggca
     1381 agatactttc gtacctggcg ttgataattt cctgcaaaat agccatttca gccgcgctgc
     1441 caaactgaac gccaacttcg tcgatgatca gcaaatccat tgacgcataa cgctcaataa
     1501 cttcatccgc tgttttttcg ctgtcattcc gccagcaatt tttcacagca cgggtaaggc
     1561 gcatcacgtc ggtgatctcc acactggcca gatagttacg gatgatgtgt tttgccatag
     1621 ccacagccag atgatttttt ccggtaccac aactgccggt cataacaaga ctggtaccgt
     1681 tctccagcat atctggccaa ttctccgcat agcggcgaca ggccgcaaga tttctggctg
     1741 cgccaggatt aacctccaga taattatcaa actcgcagtc ccgaaaacgc agggcaattc
     1801 cggcgttatc agtcagctct tccgccttga tggacgacag ctccatggtc aaatcgttgg
     1861 cctcagcgat taagcagtca gggcagcatg aaattttttc tctgtcctcg ccattacgat
     1921 cgctccacac cagtatatgc gtgtgatatt cgccatgttt ttcgcaatat ccgcgacctt
     1981 cacgcatcag gcaggaacga taaggccatg gcttttcgcc cttctgagca aatgcaatct
     2041 ctgcccgtaa ctcatccatt cgcgcctgta gtcttttttg ttgttcacgc aggttaaacg
     2101 tcatcatcgc tgtcacctca gaatgtcagt ttgtcactgg atttaccgaa tttgtcagac
     2161 atggctccca ggccagccag gacatcgacc tgtcgctgtc gcccacctcc gggagcggct
     2221 ggctgttgcc agaagtcttc gaagtgacga tcgggtccaa agaacgtcgc agcctgcttc
     2281 acgaactgtg tgccggtatt tcctgtggca cgtacccagg cagcatagcg tttcacgcca
     2341 tcaagcatgg cttcaggttt tattccctcc ctgagccggg ctttccaggc tttgaaggct
     2401 gcggattttg agttaccacc agcacgtttt ggatattcct gccaggcctg ttcaaattcc
     2461 ggtgaatatt cctgtcggga agaacgcgct ggtgcagacg cgtcagcaga tgcgccaata
     2521 gtgtttttac tctctgtagt attctctgaa gtaatctctg ttgtattctc tgtaagatcg
     2581 aaattggttt tcccttctcc gcggcgaggg gtttcccgtg tccgcggtga aggctttccc
     2641 tcctccgcga aattgggttt tacagtttcc cgaaaacggg tttccccatt tcgggaaact
     2701 gattgttttc attgataatt tcattaaggc gctcacaatc tatacggtag aacattttgt
     2761 gctcaagacg cttgttggtt tcaaccaaaa tgcctctgga cacaagatgc ttacgcgcta
     2821 cagcctgttg ttcaaatgta agtccggttt cgtgttgtat ctcttcacgc gttttatgta
     2881 cgccttccgc tgcatgtgct ttatcctacc agtaaaaaat ctgaccaaag aaaataacag
     2941 cgtgcggact tcccatgtat ttaacgagcc cagggtaata agcaaccgga tgtccaaaat
     3001 cgagcagaag atcagacgga cgcatagcca cctcccaggc gtttaaacat ttttccgggc
     3061 tgaaacgcca ccagcggata actcagggta tgagtacgtc cctgaacctg gcagacaacc
     3121 ttctggcttt cggtactgac caggcaaacc cgcagaacgt agccgttgct ggtggtgaac
     3181 cactgcccca cacgggggca atggttgtat cggtgataca ggaaattaac gatgtggcgg
     3241 atcatgggag cacctccttg tcagaaccat tcagcctgga atcaacaagt gcagcaccaa
     3301 aaacagcatc acctacacgg tcgtacagtt tgccagccag cggagattca acggccttaa
     3361 gcattgggta aagctggctt gtccagattt gatggatttc acgcaaatgc aggtatacgc
     3421 ctctggcgtt ttgtgcgaca gatgacatgt cagccgcacc agctcctgat aagctcctct
     3481 ccatctggtt aaaggcattg atgtatgcct ctttgaaccg ggcggcacgt ttaccagtga
     3541 agcccatagc aaggaaggcg aagccgtcgc gggtaatttg gtagcaggga agtttgcgag
     3601 taccgccgtt gggctggcgt accaaaattg atgtctccgc aaaattgcgg gcacaaaact
     3661 ctggagaaca atccaaaatg cggatctttt tcagaacatc gtcatgacgt ttagagaaga
     3721 agttggcaac agccagggat gaagtaacag cctgaccatc aacgatggca atttcaggtt
     3781 gagagagggt tgggagagta gtcatggtga cagcccctat gttgaattca aagaactcac
     3841 cacatgggac gccaatcaca gaggtggtga gacgtacagg gttggcgtaa ccggtcaaca
     3901 tagaacccgg cgcatcttgc gatgcccctg cacgccccac cataatttgg gcgtagcaat
     3961 gctcatgaca cgaaaaaacc gcatgagcgc ggttatgctc tatattgaat ttcaggacgc
     4021 caatcccggc acccgcttta taaggtgcag agacagtgta acgtcccgaa attgcagaat
     4081 caatatattg tgcagatata acgattttgt tatgttttgc tgtatgaact acactatcga
     4141 atactacagt gaagaggtcc gactggaggt cgatcagctt ccattgagta tgcgtgccag
     4201 ataccagcat cttgttgaac gcatgaaggt atacggcagc aatctcggag aacctcacac
     4261 cagcgccttt ggtgacgggc ttttcgaact cagaattaaa ggcaacgatg ggatcgcgcg
     4321 tgttttttac tgcactctga cagggaaacg catcatcatg ctgcatagtt ttgtaaagaa
     4381 aacgcagaaa acaccgccag ccgaacgcaa gaaagctgaa accagaatga aggaggttaa
     4441 gcatgactgg taaacgcact ctccccacca tgacacacga cgaaatggca gccaaatgga
     4501 tggaagaccc ggcttttaaa gcagaatacg acgctatcgc tgacgaattc gcactgcttg
     4561 atgaaatgct ggcagcacgc aaagaagctg gcttaactca ggctgaagtt gccgagcgaa
     4621 tgggaacaaa agcgaccgcg atcaccagaa tggaaagtaa tctcgcatca ggtatcagcg
     4681 gcccatcatt tgccacacta aaaaaattcg cccgcgctac tggaaaaaaa ctccagatcc
     4741 gcttcgttta accacaccgc gccgtcattc tggcggcgca ccggataaat agtcatttcc
     4801 tcgcacgatg tcttagccac cggatatccc acaggtgagc cgtgtagttg aaggttttta
     4861 cgtcagattc ttttgggatt ggcttgcgtt tatttctgga gcgtttcgtt ggaaggtatt
     4921 tgcagttttc gcagatgatg tcggtgaaac ttcgtcgctg tcgcctcatt catacctcct
     4981 gtcggtaaat ctgacaccct gaccaatagc ccaggctgtt gtgtactcga tcagacttgc
     5041 catgcgcttc acgctcatct gcgcgctgct ttcgcgaatg ttgacgcatt ctccttcaag
     5101 gccgggcaaa acatcagctt cctgttttgt tgccactgca tgaccgctga tcaacaaaac
     5161 cttccattgt tctggtttta accatttgcc gcaccattga acctgacgag cgatatccgc
     5221 cagcatcgcg tgaaattttg cgttctggtc aagatttcgc ttgtagtcag taatgcggat
     5281 ggtgactggc ttgtctttat cgagaggagt tgagaggatg gcatttattg cggcttgctg
     5341 ttgttgctta gttcggagga agattgtttg cttcatcgaa aattcttctc tttaattcca
     5401 gcggctctga tagctttcat tactgcaatt accgttttgt ccttcccatc ctcatgcccc
     5461 atcgcataag caccttcttc accatctttc caaaagtcgt cattcgattc gggccagtcg
     5521 atatccagtt caatagctgc tcgcggtgcc tgccatatca cccaggcaaa ctcttttaat
     5581 tcatcgtctc ctgtgaactg gcttttgtct tttgaccacc agttttcaaa ctgtcggtag
     5641 ctatcgttca cttccctctc ccccaaataa aaaggcctgc gattaccagc aggcctgcta
     5701 ttagctcagt gatgtagatg gtcatttaat actccgtcac gttttcctgt cgccacgcct
     5761 cgtcatattc cgatttcggc atattggcga tgtagctata tggcgatcct gattcaagtt
     5821 gcaggaactg gtgcgattgc tcgtcaagga acaacgggac accaccttcc caaccttcgc
     5881 cgttacgttg tttttcaagc atcaaaacag atgccggaga tgccagtagc tgttcgtcct
     5941 tctctgacat cttttcacca ctctgaactc tctgtaacgc tctctcgcga gccttgttac
     6001 gccagatgat gaaaaggttg tctgtcaggt ctgttatcgc tccagagcct tttacgtcca
     6061 ttttcccggt tggtttttct tcgctgtctc cttttcgcga gtgagtaacg agaatgacgt
     6121 gggagtttgt tttgtttttg aagtcgcaaa tcgagtcaac aaacgccttc tgcccgttat
     6181 agtcatcgtc gcctatgcca catttcatca ggctgtcgat gatgaataac tggatcccgt
     6241 atcggcggcg agcgtagtcg aatatttcga tcagcctgtc ggctttcgcc gttccagtca
     6301 ggccaaacac ccaaagtctt tcgtcataaa atttaaatgc agagtcaatt tccagcactg
     6361 gcggcatctt gcagcacgtt gcctgacggg taagtcgctt aaggagaata ccaggcttca
     6421 gctcaagtga cgcgatgcac gtcttcacac cctgacgcat tgcctcaagt gccatatgcc
     6481 cgacaacctc ggtgttatgc gtcgccacat atcccctggt tacatacagg tggcgagggt
     6541 gatcgaccat aatacacaag cattctgcat tgccgatttt ttcaacattc ctgacgaata
     6601 ccccacagcc ctcgaatcga tgcgcagtta atctgccatt gagtcgtggt gaacggatgg
     6661 cctccctgat ttctcttgtc agtctgattc ttgcctcgta tgaatccatc ccatgccgct
     6721 tatcgtcctt gtatgtgtat gtcacgccag ttttaactcg cgtccggcat gacccgccga
     6781 gtgagttaac cagttgaaca accccattgc gcagttcttc acttgctgag gaaaaaacaa
     6841 gcgttccatc cttctcgaca tacccatccg tttcaagcag accacacagc atgcctatac
     6901 gcgttgattt atttgcggaa aaaaacacac gagggatgaa tttgtttttt gctgtgcacc
     6961 ccattagtcc ataacctcgc agggtctcca tgagtggatt tacctgacca cgcgccgttg
     7021 atatcagcca gtccttacca tctccagaga agttgtaatc aggcagttca gccttcatac
     7081 gctcgatcat gtatggctcg acgtttgaaa acttcacgct cccattgcta agactaccat
     7141 ccccgagaag ggagccgata acccatgcta atggctctga gtggtcgcca aagtcaccag
     7201 ttatttcagg aatcctaacg ccatttttgt gcctcttcgt ttcactcaac cgcttcaacc
     7261 cgaaggtatc tatcacgcgg cgtttctcgc ctttcgtgaa tccacgacta gtgacctccc
     7321 ataggtgatc gcctgcgcaa tcaacataac gaccatcttc aaatgtgact cggtaaacat
     7381 ctctaacacc ctgcgggaat atcccagtga cagttgacgg attgccgtct actgacgcca
     7441 cctgatcgcc aatttttaca ttcccgtgag tagtccatgt cccatcagct aaaagaatag
     7501 gctcattcag tgggcatgcc tttccgtgac cgttcacacc attgaccagc gtcaactctg
     7561 cctcacggaa ctggaattta tctgccagag attcccacgg tggattaaac agatactgct
     7621 gcttgccgta gaaagcgttg atagtgtcct ggtaaaactc tcgcgcactg tagagttctt
     7681 caggatcgaa gtaggatgcc gtgccgatgt actgccagat ttcatcctcg gtaacaccgt
     7741 tcatcaggca ttcgttgatg tctttgtacg gcagagtaac aagacggcaa cgatgttcac
     7801 cgagtcggct tgcgatttcc cttgcggctt cacgaccaac atcatcaacg tccatcgaga
     7861 tgaatatttc ctcaaacctg tcgaggttat gatactcaaa ctcaatccac tgttgcttag
     7921 cgcctttccc gccaccaaac ggcacggata acgccgagat gccgtattgc gcatagctca
     7981 tacaatcaat ttcgccttcg caaagcacaa ccgccctcac gccagcgtcc agagcctgcc
     8041 atccgaacag gcaaggttcg caatcacct
//
LOCUS       DAJVQH010000154         7864 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJVQH010000154 DAJVQH010000000
VERSION     DAJVQH010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.154"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..313)
                     /locus_tag="OQM87_003658"
     CDS             complement(<1..313)
                     /locus_tag="OQM87_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1509634.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVALLWVNGE
                     NF"
     gene            complement(323..871)
                     /locus_tag="OQM87_003659"
     CDS             complement(323..871)
                     /locus_tag="OQM87_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCS1509635.1"
                     /translation="MGLPPLSKTPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYTEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(871..1578)
                     /locus_tag="OQM87_003660"
     CDS             complement(871..1578)
                     /locus_tag="OQM87_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1509636.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1593..2270)
                     /gene="ydjY"
                     /locus_tag="OQM87_003661"
     CDS             complement(1593..2270)
                     /gene="ydjY"
                     /locus_tag="OQM87_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCS1509637.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2275..2985)
                     /locus_tag="OQM87_003662"
     CDS             complement(2275..2985)
                     /locus_tag="OQM87_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1509638.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3152..3958)
                     /gene="xthA"
                     /locus_tag="OQM87_003663"
     CDS             complement(3152..3958)
                     /gene="xthA"
                     /locus_tag="OQM87_003663"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCS1509639.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4404..4982
                     /locus_tag="OQM87_003664"
     CDS             4404..4982
                     /locus_tag="OQM87_003664"
                     /inference="COORDINATES: protein motif:HMM:NF012428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1509640.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGSIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDATCAVIVEPI"
     gene            4998..5624
                     /locus_tag="OQM87_003665"
     CDS             4998..5624
                     /locus_tag="OQM87_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1509641.1"
                     /translation="MPASNAFLQGLRELCDRHNALLIFDEVQTGVGRTGELYAYMHYG
                     VTPDLLTTAKALGGGFPVGALLATEECARVMTVGTHGTTYGGNPLASAVAGKVLELIN
                     TPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRGLGLLIGCVLNADYAGQAKQISLEA
                     AKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDRFAAACEHFVSRGSS"
     gene            5621..7354
                     /gene="astA"
                     /locus_tag="OQM87_003666"
     CDS             5621..7354
                     /gene="astA"
                     /locus_tag="OQM87_003666"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCS1509642.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAAGWLAAGRETGQALSALEDLDGLLFTGSANTGYQLHRQLSGQPEKILALEMGGNNP
                     LIIDEVADIDAAVHLTIQSAFVTAGQRCTCARRLLLKSGAQGDAFLARLVAVSQRLTP
                     GNWDDEPQPFIGGLISEQAAQQVVTAWQQLEAMGGRTLLAPRLLQAGTSLLTPGIIEM
                     TGVGGVPDEEVFGPLLRVWRYDTFDEAIRMANNTRFGLFCGLVSPEREKFDQLLLEAR
                     AGIVNWNKPLTGAASTAPFGGIGASGNHRPSAWYAADYCAWPMASLESDSLTLPATLN
                     PGLDFSDEVVR"
     gene            7351..>7864
                     /locus_tag="OQM87_003667"
     CDS             7351..>7864
                     /locus_tag="OQM87_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCS1509643.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALL"
BASE COUNT         1851 a         2028 c         2129 g         1856 t
ORIGIN      
        1 ggaagttttc gccgttcacc cagagcagag ccaccgagcc gcccgtttta cgaccggcag
       61 cagcttcggt ctgaatgcgc ttcaccgcgt ctgcggcatc cgcgagacga acaatcttca
      121 ggtttatagc gtaatgggtt ttcatctcgc cgctaaccca gtcgagatag cggttaattg
      181 cggtatcgcc gccccaggcg ttaaaccaga cggtttgccc tttggcctca tttttaattt
      241 cctgccagtc actggcatgt gttgccaaag aaaacagcga taacaatccc agcaaccacc
      301 cacaatggcg catcttccct ccttaagaac gtttttccgg aatacggcac agcccctgag
      361 cgggaatgtc catggcagaa ttaaaacgag ccgacaggtt ttgcaggccg atcaatgccg
      421 tcagttcggt gagcgcctga gcgtcaaaat gcgcagccag tcgggtacgc agggcatcat
      481 cgactgttgg cggtgtgacg cttgcggctt cggtgtactc cagcgccagc cgttcttcat
      541 cgctaaagag cgggctttgc cgccagtcag ccactgccag cagtttatcg gtgctgccgg
      601 tacgctcggc gactttcata ctggtgatat ccacgcaaaa ctcacacagg cacatttgcg
      661 caatgcgcgc gctgacaagc gatcgcacta ccggatcgag cggtgagcgt ttgcgctcca
      721 gccacccaac aaacattgac accaaataaa agataaacgg gatccgcccc caccagcgaa
      781 tcgggcttag cacctcgccg taatggcgac gatgcagcca cgcctgcgga cgtaaaatga
      841 aaggagtttt gctaagcggc ggtaaaccca tcagtcattc ctcttctggc gctcaagcca
      901 tattttcttc gccataaaaa tcaccacggt cagggcaaac agaataaaca gttcggtgac
      961 aaaccagaaa gtaccgcctg ttaacatgct gcccgcccag gaataaacaa tagtcgccgg
     1021 taattgacca agcccggtgg cgataaaaaa cgagcgaaaa cgtattgaag tcagaccggc
     1081 agcatagctg attggatcga aagggacaaa aggcaataac cgacagacca ggatggtgtg
     1141 tttgccgtag cgagtgaaaa agccgtccat actgtcaagc acggttttgc cggttaattt
     1201 ttccaccact tcgcggccca tcactctggc gataaaaaag cacagcgccg cgccggccat
     1261 cgaactggtc cacgacagca agccgcccca gaacgcgcca aacagcgacg cattggcaaa
     1321 ggtgatcaaa aacgcaggta gcggtgcagc aatggcctgt aaaatcatca acaagaacga
     1381 gacaactgcc gccagtgcgc cgtaagactg aataaagcgt tctataccct gttggtccac
     1441 ggcggcaaac gccgcaacgc tgcgattgat aaactcatgg acgccgggaa gcagcgccca
     1501 tgcgagcagc atagcgaaca acaggatgat gagggttata cggtagtacc agattttacg
     1561 cgattgtatc ttcatcatcc tttatcctgg ctttattcgg tgattttaaa ggtaaccgtt
     1621 gccaacgtgt tatccgccgg gagaactgag gcattgcctt tgaatttcac ttcgccgcgt
     1681 ttttccaccg caccataagt gtatgttgca ttgctgacga tgccgaccgg gcagctatcc
     1741 agacacacca ggcaacccgt tttcttctct tctgctgccg ttaaattacc gccaaagcgc
     1801 atgtccagtt tcttgccatt actgtcaaca atcacttcat cgaaggaata cgcttttgcc
     1861 gccccttgcc agttgaccga aatatccagt ttgctgcctg tgacatgagt cgtttcttta
     1921 ttatccatcg tcatgttttc gcccggcgta ccacctgcct ctttcagggc ttcataaaat
     1981 gctttcgggg tcgcataagc cataaacagc gatttatgtc cgttggagcc atctttaaac
     2041 acaataccgt gacgagtgtc gtcggtgagg aaacgaccat taatttgcac cagcatggtt
     2101 accgtatggt tattggcatc gacgcgcagc ggttggctgt tcgaaagtcc gtcgtattcc
     2161 acttttgccg ccgcattctc tttttgatcg cagccgctaa gcccggcaac agccagtaag
     2221 cagagtgcag ccagtccttt tttccatgac actgaataat gttgcgacat cgttctatcc
     2281 ttcatttttt gggtgagtaa gtgggctgcg gcgcgaagca gacagatcca catgtttgtg
     2341 tcgggcgtag agttttgcga gctggatgag aataaacagc gccaggcccg ccagacagag
     2401 ttgtaaaata aagcgcagcg taatgccttc attggcgaga tcgcttgcca tcacggtata
     2461 aataacaata ccgggcaggg tcgtgagtgc cgaaataagg gtataaggcc agaaggcgat
     2521 ggtggttaat ccgtaagcgt aattttgaat attgtaagga aacaacggga ttaagcgggt
     2581 cagaataaga aaatcaatac cgttacgcgc aatgcctttt tcaatggcct ggaaggtatg
     2641 gctatggccc acgtatttca gcagtaaatc acgtcccagc cagcgcgcca gcaggaatga
     2701 gcacgaggag gccagcgttg cggcaattaa tgagagtagt gtccctaaga gcgggccaaa
     2761 aactattcct ccagcgatca ccagtatgct tcctggtaac agaaagaggg gggcattgat
     2821 gaataacaga atatagaggt tataaccgaa caatccgctc tggcgaatga gtgtctgtaa
     2881 gtggggtaaa tcggtgagca gatcgaataa accgaaagcg tggatagcgt aaatgaccag
     2941 cgcaaaaata agacaggcaa acagaaagtt acgctcagcg ttcatcgtgg agtttcctat
     3001 atcaaacttg ataatagaga agtggtcaga caactaaaaa cgcagtgacg gtataacaaa
     3061 ggacggcagg caacaaatca ttactgacag tttgttgatt tttctcaagg ttaattctcc
     3121 tgacccagtt tgagccagga gagctgctaa attagcggcg gaaggtcgcc cagacggggg
     3181 cgtgatcgga cggtttttcc atgctgcgaa tttcatagtc gatgccggtt tctacgcaac
     3241 attctgccag cggttggctg gcgagcagca ggtcgatgcg cagaccacgg ttatcgtcaa
     3301 aaccttttga gcggtaatca aaccatgaga aacgatctgc tgtttgcgga ttagcatggc
     3361 ggaaggtatc gaccaccccc cagctcatca gcctgtccat ccattcgcgc tcttccggca
     3421 ggaaagagca tttaccggta cgcagccagc gcttacggtt ttcttcgcca atgccgatat
     3481 ccagatctgt agggctgata ttcatatcgc ccataatcag taccggatta tcacgtttga
     3541 gttcggtttc caggtagttt tgcagattct gataaaactg cgcttttgcc gggaatttta
     3601 tcggatggtc gcggctttca ccctgcggga agtaaccgtt gatcacggtg acataaccca
     3661 gcagtgaggg gatttccgcc ataataatcc gccgctgcgc ctcttcgtcg tcaccgggaa
     3721 agccgcgacg cacggcaatc ggcgtctctt tggtcagcag cgccacgcca taatggcctt
     3781 tctgcccgtg ataaaacacg ttgtagccga gcttcgccac ctcttcgagc ggaaacatat
     3841 cgtcatgaac ttttgtctcc tgcaggccaa tcacatccgg ttggtgcttt tcgacgatgg
     3901 cttcaagctg gtgaggtctg gcgcgcaggc cgttgatatt aaaagagaca aatttcatag
     3961 tcgctgccat ttattcagat aaagagtgcg tggatggtag cagaatttcg cgttgcttac
     4021 cgcctgttgt taagtgattt gatcgatggt tgttaatgat gtcaacgatg gcgcaaaaaa
     4081 tgcccgcttt tggtgcgcgc tgcgtcagaa tggcgcagta atttccagta aatttcgtca
     4141 atgatcacaa ttccagaatt atattttact tctgcataac attgcgtttt ttattctttt
     4201 tattcaccca catcgcaaac gtattcactt tatatgcact ttaaatgcat atggtttggt
     4261 tataactcct tgatttccag ttagcctccg ccgtttatgc acttttatca ctggctgaca
     4321 cgaaccctgc aatctacatt tacagcgcaa acattactta ttgttaacat acaaataacg
     4381 aattatttac tgtagaggtc gctatgtctc agccaattac gcgtgaaaac tttgatgaat
     4441 ggatgatacc tgtttacgct ccggcaccct ttataccggt acgtggcgaa ggttcgcgct
     4501 tgtgggatca gcaggggaaa gagtatatcg acttcgcggg tagcattgcg gtgaacgcgc
     4561 tgggccatgc gcatccggaa ctgcgtgaag cgctgaacga acaggcgagt aagttctggc
     4621 ataccggcaa cggttacacc aacgagccgg tactgcgact ggcgaaaaaa ttgatcgacg
     4681 ccacgtttgc cgatcgcgtc ttcttttgta actccggtgc ggaagccaac gaagcggcgc
     4741 taaaactggc gcgtaaattc gctcacgacc gctacggcag ccataagagt ggcatcgtgg
     4801 cgttcaaaaa tgcgtttcat ggtcgcacgc tgtttactgt cagtgcgggt gggcagccag
     4861 cctattcaca ggattttgcg ccactgccgg cggatattcg tcatgctgca tataacgata
     4921 ttaactctgc cagcgcgctg attgacgacg ccacctgtgc ggtgattgtc gagcccatct
     4981 agggcgaagg cggtgtggtg ccagccagca acgcgttttt acaaggtctg cgtgaattgt
     5041 gtgaccgcca caatgccttg ctgatttttg atgaagtaca aaccggcgtc gggcgcactg
     5101 gtgagctgta tgcctatatg cactacggcg tgacgcccga tctgttaacc accgccaaag
     5161 cgctgggcgg cggtttcccg gtcggtgcgt tgttggcaac cgaagagtgc gcccgcgtga
     5221 tgaccgttgg cactcatggc accacctatg gcggtaatcc gctggcctcg gcggtagcag
     5281 gcaaagtgct ggagctcatc aacacaccag agatgcttaa tggcgttaaa cagcgtcacg
     5341 actggtttgt tgagcgtctt aatactatta atcaccgcta tggtttgttc agtgaagttc
     5401 gcggcttagg tttgttgatt ggctgtgtac tgaatgccga ttacgccggg caagcgaaac
     5461 agatctctct ggaagcggcg aaagcaggcg tgatggtact gattgcgggt ggcaacgtgg
     5521 tgcgttttgc gcctgcgctc aatgtcagcg aagaagaggt gacgaccgga ctggatcgct
     5581 ttgcagctgc ttgcgaacac tttgttagcc gaggttcatc atgatggtca tccgtcccgt
     5641 tgagcgctca gatgtctcgg cgctgatgca gcttgccagc aaaacgggcg gcggcctgac
     5701 gtcgcttccc gccaatgaag ccacgctttc ggcgcgtatc gaaagggcaa tcaaaacctg
     5761 gcaaggcgaa ctgcctaaaa gcgagcaggg ctatgtgttc gtgctggaag atagcgagac
     5821 aggcaccgtg gcggggattt gcgctattga ggtggcggtt gggctgaacg atccctggta
     5881 caactatcgc gtcggcacgt tggttcacgc ctcaaaagag ctgaatgtct ataacgcatt
     5941 gccgacgctg tttcttagta acgatcacac cggcagcagc gagctgtgca cgctgtttct
     6001 cgacccggac tggcgcaaag agggcaacgg ctatttgctg tcgaaatcgc gctttatgtt
     6061 tatggcggct tttcgcgaca agtttaatga caaagtggtt gctgaaatgc gcggggtgat
     6121 tgacgaacac ggctattcac cgttctggca aagcctcggt aaacgcttct tttcgatgga
     6181 ttttagccgc gccgattttc tctgcggcac cgggcaaaag gcatttattg cagaactgat
     6241 gccgaaacat ccgatctata cccacttttt atcccaggaa gcccaggacg tcatcggtca
     6301 ggtacatccg caaaccgcgc ctgcccgcgc ggtgctggag aaagaaggtt ttcgttaccg
     6361 caactatatc gacatcttcg acggcgggcc aacgcttgag tgtgacatcg accgcgtgcg
     6421 cgccatccgt aaaagtcggc tggtggaagt ggcagcaggc tggcttgccg ccgggcgtga
     6481 aacgggtcag gcgctgagtg cgctggagga tctcgacggt ttgctgttta ccggtagcgc
     6541 caataccggc taccagctgc atcgccagct ctccggtcag ccggagaaaa ttctcgccct
     6601 tgagatgggc ggtaataatc cgctaattat cgatgaggtg gcggatatcg acgcggctgt
     6661 ccatctgacc attcagtcgg cgtttgtcac agccgggcaa cgctgcacct gcgcccgccg
     6721 tttattgctc aaaagcggag cgcagggcga tgcgtttctt gctcgtctgg ttgccgtcag
     6781 ccagcgatta acgccgggca actgggatga cgaaccgcag ccgtttattg gcgggctgat
     6841 ttctgaacag gccgcacagc aggtggttac tgcctggcag caactggaag cgatgggcgg
     6901 acgaaccctg cttgcgccgc gcttattaca agcagggaca tcgttgctga caccagggat
     6961 cattgaaatg acgggcgttg gcggtgtgcc ggatgaagag gtgttcgggc cgcttttgcg
     7021 cgtctggcgt tatgatactt ttgatgaggc gattcggatg gcgaacaaca ctcgcttcgg
     7081 actcttttgc ggtctggttt cccccgagcg ggaaaaattc gatcaactgt tgctggaggc
     7141 gcgggcgggg attgttaact ggaacaaacc gcttactggt gctgccagta ccgcgccatt
     7201 cggcggcatt ggtgcttccg gtaaccatcg ccccagcgcc tggtatgccg cagattactg
     7261 cgcatggcct atggcgagcc tggagtcgga ctcgttaaca ttgcccgcca cgcttaaccc
     7321 cgggctggat ttttccgatg aggtggtgcg atgaacgcct gggaagttaa tttcgacggg
     7381 ctggtagggc tgacgcatca ttacgcgggc ctgtcgtttg gtaatgaagc ctctacccgt
     7441 caccgttttc aggtgtctaa cccgcgactg gcggcaaaac agggcttact gaaaatgaaa
     7501 gcccttgccg atgcgggatt cccccaggcc gtgatcccgc cgcacgagcg tccgtttatt
     7561 ccggtgctgc gtcagttggg attcagtggt agcgatgagc aggtactgga aaaagttgca
     7621 cgccaggcac cgcactggct ttccagcgtc agttccgctt cgccaatgtg ggtagccaat
     7681 gcggcaacga tcgcgccatc tgccgatacg ctggatggca aagtgcatct caccgttgcc
     7741 aacctgaaca ataaatttca ccgttcgctg gaagcgcccg tcactgaatc gctgttaaaa
     7801 gcgattttta aggacgaaga gaaatttagc gtccattcgg cgttgccgca ggtagcgttg
     7861 ctcg
//
LOCUS       DAJVQH010000155         7854 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJVQH010000155 DAJVQH010000000
VERSION     DAJVQH010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.155"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="OQM87_003668"
     CDS             complement(<1..141)
                     /gene="yjdI"
                     /locus_tag="OQM87_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCS1509644.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            322..1953
                     /gene="dcuS"
                     /locus_tag="OQM87_003669"
     CDS             322..1953
                     /gene="dcuS"
                     /locus_tag="OQM87_003669"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCS1509645.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1950..2669
                     /gene="dcuR"
                     /locus_tag="OQM87_003670"
     CDS             1950..2669
                     /gene="dcuR"
                     /locus_tag="OQM87_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCS1509646.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3240..4580
                     /gene="dcuB"
                     /locus_tag="OQM87_003671"
     CDS             3240..4580
                     /gene="dcuB"
                     /locus_tag="OQM87_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCS1509647.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4658..6304
                     /gene="fumB"
                     /locus_tag="OQM87_003672"
     CDS             4658..6304
                     /gene="fumB"
                     /locus_tag="OQM87_003672"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1509648.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAVIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6421..7050
                     /locus_tag="OQM87_003673"
     CDS             6421..7050
                     /locus_tag="OQM87_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCS1509649.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7189..>7854)
                     /locus_tag="OQM87_003674"
     CDS             complement(7189..>7854)
                     /locus_tag="OQM87_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1509650.1"
                     /translation="FAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRR
                     ILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADTVD
                     YGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQF
                     IMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSD
                     VKA"
BASE COUNT         2032 a         2004 c         1930 g         1888 t
ORIGIN      
        1 cgactttcgt ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg
       61 acatatcgca gtgttgaaat agacatcgat cttttcgccg gtataacagc gataaccccc
      121 gtccagtagc gcctgatcca tgaccttccc tctctcatca gtatgattat cgctataagc
      181 atagccccgg aatcttcata cagcactggc agtttccgtt gccagagtct cttcccctat
      241 atagccaatc atttttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa
      301 atttgtcaca caaggaagct gatgagacat tcattgccct accacatatt acgcaaacgt
      361 ccgatgaaat tgagtaccac tgtgatctta atggtcagcg cggtactgtt ctcggtgcta
      421 ttggtggtgc atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctggcc
      481 aacaaggcac tggcagtggc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg
      541 cagaaaaaac cgcaggagag tggcatccag gccatcgcgg aagccgtgcg caaacgcaac
      601 gatctgctgt ttattgtcgt taccgatatg caaagtcttc gctactcaca tcctgaagcc
      661 cagcgtattg gtcagccatt taaaggtgat gacatcctta atgcgctgaa tggcgaagaa
      721 aatgtcgcta tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac
      781 gatgaaaatc ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc
      841 caacagatca atgacagtcg ctggagcatt atctggtcgg tattatttgg catgctggtc
      901 ggactgattg gcacctgcat tctggttaag gtactgaaaa aaatcctttt cggcctggaa
      961 ccctacgaaa tctccacgct gtttgagcaa cgccaggcca tgttgcagtc tatcaaagaa
     1021 ggcgtcgttg ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa
     1081 ttgctgaatt accgtaagtc gcaggacgat gagaaactgt cgacgctcag ccactcatgg
     1141 tcacaggtgg tagatgtctc ggaagtgtta cgcgacggta cgccgcgccg cgacgaagag
     1201 attacgatta aagaccggct attactgatc aacaccgttc cggtgcgcag taatggcgtt
     1261 atcatcggtg ccatttcaac cttcagggac aaaactgaag ttcgtaaact gatgcagcga
     1321 ctcgacggtc tggtcaacta tgctgacgca cttcgtgaac gatcccacga atttatgaat
     1381 aaattgcatg tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac
     1441 attctcaaaa cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa
     1501 tctccggtta tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat
     1561 acgctgattt taaacagtga aagccagctg ccagacagcg gcagtgagga ccaggtcgcg
     1621 acgctgatta ccacattggg aaatctgata gaaaacgcgc tggaagcatt agggccggaa
     1681 cccggtggcg aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt
     1741 aacgatgatg gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc
     1801 tcgacaaaag gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat
     1861 ctcggcggca gcatcgccgt ggaatcggaa ccggggattt tcacacaatt ttttgtccag
     1921 ataccctggg acggggagag gtcgaacaga tgatcaatgt attaattatc gatgacgacg
     1981 caatggtcgc ggagctgaat cgccgatacg tagcacaaat cccaggcttt caatgctgtg
     2041 gaacagcctc gacgctggag aaagccaaag agattatctt caatagcgat gcgcctatcg
     2101 acctgatatt gctcgatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc
     2161 tgcataacgc gcgttgcaaa agtgatgtga ttgtcatctc ctccgcagcc gatgcggcaa
     2221 ccattaaaga ttcgctgcat tacggtgtcg tggattacct gatcaaaccc ttccaggctt
     2281 cccgctttga agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc
     2341 agtattatga ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgaacagg
     2401 atcctcgtcg cttgccaaaa ggcttaacgc cgcagacgtt acgcacgctg tgccagtgga
     2461 ttgacgcgca tcaggactat gacttttcaa ccgacgagct ggctaacgag gttaacatct
     2521 cgcgtgtttc ctgccgaaaa tacctcatct ggctggtcaa ctgccacatc ttgttcacca
     2581 gtatccatta tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact
     2641 actcactgct gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta
     2701 ttaccgtaat gcaaaatacc cctggcacaa gttacatcgt taatcagtaa aacttgtgcc
     2761 agatcaaata taattatccc tccatcatcg ttaaaaatta atatctcttc agatgaacgg
     2821 tgtttttaat ttcaaaacga taacaaaagt taattaacta ttatgtcacc cgcattatgt
     2881 gtatttttac ccacaaatgg gtagatcaga ttaatctata aacctaatga tatctgccct
     2941 gagaacaaaa aatagaccga taaatatcaa taagataaca gcaaacaaaa cattaacatc
     3001 tgcgcagtac aaactataaa cccatcgcca gagagttttt ctctctgaaa aagccgctta
     3061 tcacagtgca taaatttgcc gctgctttaa tcagccaata ttcactgtga ggtatttgct
     3121 aaagccggta acgaccaaac ggatatttag tcaggctctg aaaacagttc atacaaaaca
     3181 gaacgtgact gtgatctatt cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca
     3241 tgttatttac tatccaactt atcataatac taatatgtct gttttatggt gccagaaagg
     3301 gtggtatcgc gctgggttta ttaggcggta tcggtctggt cattctggtc ttcgtcttcc
     3361 accttcagct aggtaaacca ccagttgatg tcatgctggt tatcattgcg gtggtggcgg
     3421 catcggcgac cttgcaagct tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc
     3481 tgctgcgccg caacccgaaa tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacactga
     3541 ccattctttg cggtacgggt catgtggttt acaccattct gccgatcatc tacgacgtcg
     3601 ccattaagaa caacatccgt ccggaacgtc cgatggcggc aagttctatc ggtgcacaga
     3661 tggggattat cgccagtccg gtgtcggttg cggtcgtgtc tctggttgcg atgctgggta
     3721 atgtaacctt tgatggtcgc catcttgagt tcctcgatct gctggcaatc accattccat
     3781 cgacgttaat cggtatcctg gcgatcggta tcttcagctg gttccgcggt aaagatctgg
     3841 ataaagacga agagttccag aaattcatct ccgtaccgga aaaccgtgag tatgtttacg
     3901 gtgataccgc gacgctgctg gataaaaaac tgccgaaaag caactggctg gcaatgtgga
     3961 ttttcctcgg ggcaatcgct gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat
     4021 ccttcggcgg caaaccgctg tcgatggtac tggttattca gatgtttatg ctgctgaccg
     4081 gggcgctgat tattatcctg accaaaacca atcccgcgtc tatctcaaaa aacgaagtct
     4141 tccgttccgg tatgatcgcc atcgtggcgg tgtacggtat cgcatggatg gcagaaacca
     4201 tgttcggtgc gcatatgtct gaaattcagg gcgtactggg tgaaatggtg aaagagtatc
     4261 cgtgggcgta tgccattgtt ctgctgctgg tttccaagtt tgtaaactct caggctgcgg
     4321 cgctggcggc gattgttccg gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg
     4381 cttcagcacc ggcttgctac ggttattaca tcctgccgac ttatccgagc gatctggcag
     4441 cgattcagtt tgaccgttcc ggcaccaccc acatcggtcg cttcgtcatc aaccacagtt
     4501 ttattctgcc ggggttgatt ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg
     4561 cgatgtacgg gttcttataa atgcactttg cgtgccgccc gtgactacgc ggcacgccat
     4621 tttcgaataa caaatacaga gttacaggct ggaagctatg tcaaacaaac cctttatcta
     4681 ccaggcacct ttcccgatgg ggaaagacaa taccgaatac tatctactca cttccgatta
     4741 cgttagcgtt gccgacttcg acggcgaaac catcctgaaa gtggaaccag aagccctgac
     4801 cctgctggcg cagcaagcct ttcacgacgc ttctttcatg ctccgtccgg cacaccagaa
     4861 acaggttgcg gctattcttc acgatccaga agccagcgaa aacgacaagt acgtggcact
     4921 gcaattctta agaaactccg aaatcgccgc caaaggcgtg ctgccgacct gccaggatac
     4981 cggcaccgcg gtcatcgtcg gtaaaaaagg ccagcgcgtg tggaccggtg gcggtgatga
     5041 agaagcgctg tcgaaaggcg tctataacac ctatatcgaa gataacctgc gctactcgca
     5101 aaacgcggcg ctggacatgt acaaagaggt caacaccggc actaacctgc ctgcgcaaat
     5161 cgacctgtac gcggtagatg gcgatgagta caaattcctt tgcgttgcga aaggcggcgg
     5221 ctctgccaac aaaacgtatc tctaccagga aaccaaagcc ctgctgactc ccggcaaact
     5281 gaaaaacttc ctcgtcgaga aaatgcgtac cctcggtact gcagcctgcc cgccgtacca
     5341 tatcgcgttt gtgattggcg gtacgtctgc ggaaaccaac ctgaaaaccg tcaaattagc
     5401 aagcgctcac tattacgatg aactgccgac ggaagggaac gaacatggtc aggcgttccg
     5461 cgatgtccag ctggaacagg aactgctgga agaggcccag aaactcggtc ttggcgcgca
     5521 gtttggcggt aaatacttcg cgcacgacat tcgcgttatc cgtctgccac gtcacggcgc
     5581 atcctgcccg gtaggcatgg gcgtctcctg ctccgctgac cgtaacatta aagcgaaaat
     5641 caaccgcgaa ggtatctgga tcgaaaaact ggaacacaat ctaggtcagt acattccaca
     5701 agaactgcgt caggccggtg aaggcgaagc ggtgaaggtc gaccttaacc gcccgatgaa
     5761 agagatcctc gcccagcttt cgcaatatcc ggtttccact cgtttgtcgc tcactggcac
     5821 cattattgtg ggtcgagata ttgcacacgc caagctgaaa gagctgattg acgccggtaa
     5881 agaacttccg cagtacatca aagatcaccc gatctactac gcgggtccgg cgaaaacccc
     5941 tgccggttat ccatcaggtt cacttggccc aaccaccgca ggtcgtatgg actcctatgt
     6001 ggatctgctg caatcccacg gcggcagcat gatcatgctg gcgaaaggca accgcagcca
     6061 gcaggttacc gacgcgtgtc ataaacacgg cggcttctac ctcggtagca tcggcggtcc
     6121 ggcggcggta ctggcgcagc agagcatcaa gcatctggaa tgcgtcgctt atccggagct
     6181 gggtatggaa gctatctgga aaattgaagt agaagatttc ccggcgttta tcctggtcga
     6241 tgacaaaggt aacgacttct tccagcaaat cgtcaacaaa cagtgcgcga actgcactaa
     6301 gtaacctctt cggcccagca cctggcagca tgctgccagg tgctgggcca cctcttttgc
     6361 gattgtaatt tcatgcttgc tggtgaatag tcagtatttt ccctgacttg cgaactcacc
     6421 atgacccgca cactcaagcc gttaattctt aacaccggcg cactggcgct aacgttaatc
     6481 ctgatttata ccggcatttc ggcccatgac aaactcacct ggctgctgga agtgacaccg
     6541 gtgattatcg tcgtgccgct actacttgcc accgccagac gttacccgtt aacgctgctg
     6601 ctctatacgc tcatcttctt tcacgccatc atcctgatgg taggcgggca gtacacctac
     6661 gcgaaagtcc ccgttggttt tgaggtgcag gaatggctgg ggttgagtcg taatccgtat
     6721 gacaagctgg ggcacttttt ccaggggctg gtgcctgcac tggtggcacg agaaattctc
     6781 gttcgcggga tgtacgtgcg cggacgtaaa atggtggcgt ttctggtctg ttgcgtggcg
     6841 ctggcgataa gcgcgatgta tgaattaatc gagtggtggg cggcactggc gatgggccag
     6901 ggagcggatg attttctcgg tacccagggc gaccagtggg acacgcaatc cgatatgttc
     6961 tgcgcgctgc ttggcgcatt aacgacagtg atattgctcg ctcgctttca ttgccgccag
     7021 ctacggcgct atggcttgat aaccggataa caacgtgcac tttgttgatg ccggatgcgg
     7081 cgtgaattcc ttatccggtc tacaaaattt gcaaatccaa agcattacat taagacgtgg
     7141 cctgataaac atatcgcatc aggccatttt cgttagctat tgtccacgtc aggctttcac
     7201 atcactcact gcaccgaccg gaatatcagc atgaacaggc tcgggcggta ctttgcgata
     7261 tttatccagc agatgaatct ggatcctgcg cagcgtgtcg ccattgaggc gatagaaacg
     7321 gaagtagaga atcagcgtta ccatgaaaaa cagagtcggt agagcaatca taataaactg
     7381 catacctaat agggcttgcg tagactgttc aacattcggt acatagccaa tcatccctaa
     7441 cacaaccgca atgaaaaaag ccgcaaaggc tgaaccgccc ttcaccacca tagtctgcac
     7501 ggagtaagcg atgctttcac agcgtacgtg cagtttatat tcaccgtaat caacggtatc
     7561 tgccaccatg atgacctgta atacccagaa aagcgccgtt cccacattca gcaaaatacc
     7621 cgcaatcaca atgaggacga cgttgtgata gctcattaat gccattaaca ggagaacacc
     7681 acagcttaac accggaagaa tagatgctcc ggcccataaa atgcgtcggg ataatgattt
     7741 aactaagcgt gggaagaata ctaacgtcac caggttagca gctcccgcat acgacagata
     7801 ataggggaac aaatccgcat caccgataac atatgagaaa taatagatag caaa
//
LOCUS       DAJVQH010000156         7735 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJVQH010000156 DAJVQH010000000
VERSION     DAJVQH010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.156"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..178)
                     /locus_tag="OQM87_003675"
     CDS             complement(<1..178)
                     /locus_tag="OQM87_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1509651.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQI"
     gene            complement(243..791)
                     /gene="fimA"
                     /locus_tag="OQM87_003676"
     CDS             complement(243..791)
                     /gene="fimA"
                     /locus_tag="OQM87_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCS1509652.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1272..1868)
                     /gene="fimE"
                     /locus_tag="OQM87_003677"
     CDS             complement(1272..1868)
                     /gene="fimE"
                     /locus_tag="OQM87_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCS1509653.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(2346..2947)
                     /gene="fimB"
                     /locus_tag="OQM87_003678"
                     /pseudo
     CDS             complement(2346..2947)
                     /gene="fimB"
                     /locus_tag="OQM87_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            4401..5117
                     /gene="nanC"
                     /locus_tag="OQM87_003679"
     CDS             4401..5117
                     /gene="nanC"
                     /locus_tag="OQM87_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCS1509654.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5137..6243
                     /gene="nanM"
                     /locus_tag="OQM87_003680"
     CDS             5137..6243
                     /gene="nanM"
                     /locus_tag="OQM87_003680"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCS1509655.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNRWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKEEYYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            6308..7288
                     /locus_tag="OQM87_003681"
     CDS             6308..7288
                     /locus_tag="OQM87_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCS1509656.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2312 a         1622 c         1610 g         2191 t
ORIGIN      
        1 tgatttgccc catattgacc gtcatttgtt tatcaccggc ttcaatccgg caagtttccg
       61 caataatgac gccctgaaat tgcatatttc cgccgggcag cgtggtattc catttatttc
      121 cggccagagc aaacattggc agcaacgacg ctaacagaaa taatctcttc cttttcatcg
      181 ttatttttat cgcacaaggg tgggcatccc tgcccgtaat gacgtccttg aacctgggta
      241 ggttattgat actgaacctt gaaggttgca tccgcattag ctgcacccgg ggttgcctcg
      301 ccgattgcat aataacgcgc ctggaacgga atggtgttgg taccgttatt cagggttgtt
      361 tgtgcactaa atgtcgcacc gtccagcgtc agagcattgc ctgttctgtc caggatctgc
      421 acgccaacgt ttgttgcact acctgcagca gaactctgca gagctagtac atcagtacgc
      481 gtagcatcaa ttgccgtacc taagaaggca acagcggctt ttgtggcaac agtggtatcg
      541 caatcattca gctgaatgtt aaaaccaacg gcagagctgg ttgctccagc ctgcttcagg
      601 ctagcggtac gaacctgtcc caactgaacg gtttgatcaa cagagcctgc atcaactgcg
      661 caagcggcgt taacaacttc ccctttaaag ttaatggtcc caccatttac cgtcgtagta
      721 tcggccagag ccgctgcgga actgagggac agagccgaca gaacaacgat tgccagagtt
      781 ttaattttca tgctgctttc ctttaaaaaa actatttcta aatcgacatg ggcagtcgtt
      841 ctgtacactt tgttttgtca acgagtttga ctgccaacac tgcacagttt tcccccaaaa
      901 gatgaaacat ttggggccat tttgactcat agaggaaagc atcgcgcaca aactttttca
      961 gtttatttgt tggcttaata ttctattgtt atctttattt atagattttt atattgcatg
     1021 aggtggtttt tggagagaag aatgaggaag atgcgtcgag ccacagaaac gttagcttta
     1081 catatagcgg aggtgatgtg aaattaattt acaagagaaa taatttacat atcaaatagt
     1141 tagatgcttt ttgtcgtttt ttaatatttt tatgcttgag aaaaaatacg taacttattt
     1201 atgatatgga cagtttggcc ccaattgtct tgtgtttatt tgttttttaa ctttattatc
     1261 aataagttaa atcaaacctc ttctcttttt aatttttcgt ttatgagatt atttctttcc
     1321 cataatccgg caaaacgagc agcattactg gcggtataac gcacagtatg gcgaatattt
     1381 cgatgcccga gataatcctg aattaaacgg gtatctgcac cacgctccgc cagttcataa
     1441 ccgcaagcat gccttaacat atgaggatga gtctgcgtta cggttccagc ttcaataccg
     1501 gcatcgcgaa taatgcgata ggcctgctgg cgagaaagcc gactcccgcg gcgagaaata
     1561 aatatagcgt cggtccggtc agcgcctttc cagttagcac gttcctgggt ccagcgttcc
     1621 acggcttcac gctcatcaaa acgtaacggg tgaacggtag aaaatccgtt cttcagtcgg
     1681 cgaatattta ttcgaccttc attaaggtca aggtcctgat aatgcagatc aagcagttca
     1741 ctaatacgca tcccatgccg atatgccaac agaataagac aataatctct ggctcccgtt
     1801 gccccgtaac aaaccgcctg catcatggcc tgaacttctt taccggtaag ataacgacgt
     1861 ttactcacaa tagtagtact cctaactgag atatatttga atgaatacct ataggaaacc
     1921 tcaatcggtc aaaattagcc caatagataa aaagataacc acacacgaag ttatgtggct
     1981 atcagtcaat tacattaaac atcatacaaa aataaaatat cactgatgtg acaacatcta
     2041 ttttcattga tttgattcaa tataacaata gattacagag gattaacttc cagttactta
     2101 aaaaataaag aaaaaagtgt ggaaacagga gaatttaagt gatgtgtaaa cttatgaatg
     2161 attttgttta tttattttga agaacataaa ccattagatt ttgtatgggt atggttaact
     2221 taatgtttat tatgataatt aagaatcaca cttacaataa catcaattaa taaatggtta
     2281 atcatttttg atatatcgta agaataatgt agtttttaac accatccctg gtatctcaac
     2341 tatctctata aaacagcatg acgctgtcgt cctctggctc tatcccagat gccgtaaaaa
     2401 cgccctgcat tgctggcggt ataccagaca gtatgacgaa tattgcgatg cccaagataa
     2461 tcctagataa gtcgcgtatc tattcctata ttcgccaaag caaaaccaca cgaatagcgt
     2521 aacatgtgcg gatgaatctc cagtgacaac ccggcattac caccagaagt cgagataata
     2581 tggtaaaact gttgccgaga aagcggattc cccttacgtg ataaaaatac ccactcgctc
     2641 tcagcatgcg gataagaagt acggatactc aaccagtttt ttaaagcctg aacttctttg
     2701 ttcaatagcg ggtgcgttgt tgaaaagcct ttttttaatc gatggatata tatacacttt
     2761 gctttaagat caatatccga aatcctcaat cgacaaattt cactcgcccg gaaaccatga
     2821 ataaaacaaa gcaaaatcag acaataatta cgtgttgcat gaggccggta tttgctgctt
     2881 taaggagtga ctcgatttca ctatgggtaa ggaagttcct ttttttgtta tcagccttat
     2941 tcctcatcgt tttcccttat aattacagac gcgcactagc tgtgctgggt tagcaataat
     3001 ccaacatttt tatcctgatt atgtttataa aagcgaacgt ttgcttaata acttacgtaa
     3061 gtggaccagt tcttctgagc gaacttaaat ggagtagcga tagttactta taataacttt
     3121 gccatacaaa cgccatgctc ttgcatgcta tgtaccttta tatatttatc aatctgatac
     3181 gaatctaata ataaccctca tcattaatgc aactaatctt atctatatat catgtgatat
     3241 gttctgtaac aagtaaaatc aacataaaaa attcagcaaa atttaatatt tgttcaacta
     3301 gccgtctcaa atatgacatt attagttaac tgcatttgcc acaaaatggc tatttcatgg
     3361 aacgcagtaa tttcttagtg gctattatca tgctaattct tagatcccta gcgattattc
     3421 ctgcctggtt cgtcagctta ggccatgaga ttgttacatt cctttttatc ctggagatat
     3481 gggggaacgc aggcgacgct ttttcatcta catccgttgg taaatatagc tttggtcact
     3541 acgacttaag ccttcctcaa tcacagctat tcgccgggcg atacggcaaa caaactaagt
     3601 gaaacatcat cctgaaaaca ccaacatcaa caagcctctc cagatcgaca tcaggagtga
     3661 ccagttacaa gccacaaacc aagaaacata tttcatgatg agaattatgc tcagcctgat
     3721 ggcgagtaaa cccttatttt caccactctc actgagttag cttgcaaatc tttctttgtt
     3781 tagattctaa ataaattttt tataaaacat atagttaaaa gaaaatcttt agtttttgaa
     3841 gaccaccgca agtgttcgtc tggcttcata tggcatcttc ttcttagaaa aagatcgaca
     3901 tattttgtga cgcgaattgc aaatctggtt ttgttgtatg gattgcgtga tttttgatct
     3961 ggtataacag gtataaagat gcaccacgat agtcaatgag actgggcatc tcgtaatcta
     4021 tggcaaacat cacttcagtt ctttcacatc gggtgatgaa aatgcacttc agtctgaaag
     4081 gaatatgaaa atgagatcaa cagacactct attttatgac tctgggtaaa atgggttgag
     4141 taagtgatat aacttacgaa tattcaaatc aattaaaaat cagaagagat tttatactca
     4201 ggtatttaat ctggatctct gtttatttaa ataatgttaa aagagaattt tcacaggaga
     4261 ccttatacaa aaaatataaa atacagttac ccgttgccaa agacactata agcctggcaa
     4321 aaaaatatta cacaacataa atgctaattg tttatgcggg ctttgtattg ctttctgtat
     4381 cctacaaatg agtgaaattt atgaaaaagg ctaaaatact ttctggcgta ttattactgt
     4441 gcttttcgtc cccattaatt tctcaggctg cgacactgga cgtacgtggt ggatatcgta
     4501 gtggaagcca cgcctatgag actcgactca aagtcagtga gggatggcaa aatggatggt
     4561 gggcaagcat ggaaagtaat acctggaata ccattcatga taataaaaag gaaaatgccg
     4621 cactcaatga tgttcaggtt gaagttaatt acgcaattaa acttgatgat caatggacgg
     4681 tgcgcccggg aatgttaacg cattttagca gcaacggcac acgctacgga ccctacgtaa
     4741 aactgtcctg ggacgcgaca aaagatctta attttggcat tcgctatcgt tacgactgga
     4801 aagcttaccg acaacaagac ttatccggtg atatgtctcg tgataacgtt catcgttggg
     4861 atggatatgt cacttaccat attaatagtg atttcacctt cgcatggcaa acgacgctat
     4921 acagcaaaca gaatgattat cgctatgcaa accacaagaa atgggcgacg gaaaatgcct
     4981 ttgttctaca ataccatatg acgcccgata ttacgccata catagaatat gactaccttg
     5041 accgccaggg tgtttacaac ggcagagata atttatcgga aaacagttat cgcattggtg
     5101 tgtcatttaa actgtagtag acaggagaca gtcacaatga ataaaactat aatggcgctt
     5161 gctatcatga tggcttcatt tgccgcaaac gcgtctgtat taccggaaac tcctgtgcca
     5221 tttaaaagtg gtaccggagc aattgataac gacactgtct acattggttt aggtagcgca
     5281 ggtacagcat ggtacaagct agatacacag gccaaagata aaaaatggac tgcgttagct
     5341 gcattccctg gtggaccgag agagcaagca acctctgcat ttattgatgg caatctgtat
     5401 gtgtttggcg gcattggcaa aaacagcgag ggattgactc aggtatttaa tgacgtacac
     5461 aaatacaacc ccaaaaccaa tcgctgggtt aaattgatgt cgcacgcgcc gatgggcatg
     5521 gcgggtcatg tgacttttgt acacaacggc aaggcttatg ttactggcgg tgttaaccag
     5581 aatatcttca atggctattt tgaagatctc aacgaggctg gaaaagattc aaccgctata
     5641 gataaaatca acgcccacta ttttgacaaa aaagaagaat attatttctt caataagttt
     5701 ctgttgtctt ttgatccctc aacacagcaa tggagttacg ctggcgaatc gccctggtac
     5761 ggaacggctg gtgcggcggt tgtgaataaa ggtgataaaa cctggcttat taatggcgaa
     5821 gccaaaccag gattgcgaac ggatgccgta tttgaacttg atttcaccgg taataattta
     5881 aaatggaata agcttgatcc cgtctcatca ccagatggcg tcgctggcgg ttttgcgggg
     5941 ataagcaatg attctcttat atttgccgga ggggccggat tcaaaggttc acgagaaaat
     6001 taccagaacg gtaagaacta tgcgcatgaa ggcctgaaaa aatcatatag cactgatatt
     6061 catctttggc ataacgggaa atgggataaa tcgggtgaat tatcgcaagg tcgggcctac
     6121 ggagtatcat tgccctggaa taatagttta ttaattattg gcggtgaaac tgcaggcggc
     6181 aaagcggtga cggattcagt tttaatttct gtgaaggata ataaagtcac agtacaaaac
     6241 taacgcttca gggccccggt aaaggggccc tgctatcaat ttgtcatttg tattacgcga
     6301 aaattatatg aacgcaataa tatcgcccga ttattactat gttcttaccg ttgctggtca
     6361 gtctaatgcc atggcgtatg gcgaaggact gccattaccg gacagggaag atgcgcctca
     6421 ttccagaatt aaacaattag cgagatttgc gcatacgcat cccggaggcc cgccatgtca
     6481 ctttaacgac attattccac tgactcactg cccacacgat gttcaggata tgcagggtta
     6541 tcaccatcct ctggcaacga atcatcaaac acagtacggc accgttggcc aggcactgca
     6601 tattgcacgg aaattactgc ccttcattcc tgataatgca gggattctta tcgttccgtg
     6661 ttgccgtggc ggatcggctt ttaccgcggg cagcgaaggg acatattcag aacggcacgg
     6721 agccagccat gatgcttgtc gttggggaac ggatactccg ctgtaccagg atttagtcag
     6781 cagaacgcga gccgcactgg caaaaaatcc gcagaacaaa ttcctcggcg tatgctggat
     6841 gcaaggcgaa tttgacttaa tgaccagtga ctacgcgtca caccctcaac actttaatca
     6901 tatggttgaa gcctttcgta gggatctaaa acaataccat tctcagctta ataatattac
     6961 tgacgcgccg tggttttgcg gcgataccac ctggtactgg aaagaaaatt tcccgcattc
     7021 gtatgaagct atttatggca attatcaaaa taatgtttta gccaatatta ttttcgtcga
     7081 cttccagcaa caaggtgaaa gaggactgac gaacgcgcct gatgaagacc cggacgattt
     7141 aagcacggga tattacggtt cagcgtaccg gtcaccggag aactggacga cggcactgcg
     7201 aagcagtcat ttcagcacgg cagcccgtcg ggggattatt tctgacagat ttgtagaagc
     7261 aattttgcag ttttggcgcg aaaggtgagc ggggagttta ttcatggggg atgtccttgt
     7321 tcgttctcag agtagacaac aaaaaacaaa tgagatcaag gtaatatctc ttcacttgcg
     7381 ggctcgaaca gcaggcaatt gccaatataa taatctggca caagggtgaa aaacgcgcac
     7441 cgcataaacc gttgttattg cttacagatt agccggatgc cttaatagac atccgcgcct
     7501 tttcgattat ggctcggaaa tctacgaaca tctacacagt ttactggaac gttttgggcc
     7561 acagcgttcg cagcaccgtc ctgatatgcc gttctggcga ttataagtcg acggattctg
     7621 gcaaatagac gatgatgata tgcttaatga tgttcaaaga aattaggcca ttattatatt
     7681 attgcttgca ttttctttca ttcctaaaag cacccgccat taatcccagg cgcat
//
LOCUS       DAJVQH010000157         7722 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJVQH010000157 DAJVQH010000000
VERSION     DAJVQH010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.157"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..165
                     /locus_tag="OQM87_003682"
     CDS             <1..165
                     /locus_tag="OQM87_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCS1509657.1"
                     /translation="THYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFLVHITLGVWW
                     WNMLIGWNML"
     gene            348..2528
                     /locus_tag="OQM87_003683"
     CDS             348..2528
                     /locus_tag="OQM87_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCS1509658.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2669..3952)
                     /locus_tag="OQM87_003684"
     CDS             complement(2669..3952)
                     /locus_tag="OQM87_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCS1509659.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4087..5157)
                     /locus_tag="OQM87_003685"
     CDS             complement(4087..5157)
                     /locus_tag="OQM87_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1509660.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(5135..5353)
                     /locus_tag="OQM87_003686"
     CDS             complement(5135..5353)
                     /locus_tag="OQM87_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCS1509661.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(5438..6061)
                     /locus_tag="OQM87_003687"
     CDS             complement(5438..6061)
                     /locus_tag="OQM87_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCS1509662.1"
                     /translation="MTSQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            complement(6383..7090)
                     /locus_tag="OQM87_003688"
     CDS             complement(6383..7090)
                     /locus_tag="OQM87_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCS1509663.1"
                     /translation="MAQLYGTDVVRIRQNHHENKSRFVEGVHFYKVVGDELRALKHKV
                     ALNYFVKSAPNARHLILWTERGAARHAKMLETDQAWDVFEKLEDGYFNQREKVANHPQ
                     PVPQPESSLDRARAMEIALNVADRLVNMFPNLSSESKQAAVAGVFNPIVGRDVVPLPA
                     ISEHYYSATEVGQRYGISANKVGRIANTYMLKTEKYGKWFIDKSAHNDKQVETFRYNE
                     AGVHKIEELIEGERKAA"
     gene            complement(7038..7472)
                     /locus_tag="OQM87_003689"
     CDS             complement(7038..7472)
                     /locus_tag="OQM87_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCS1509664.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIGAVVRNGCCAHPAKPS"
     gene            complement(7545..>7722)
                     /locus_tag="OQM87_003690"
     CDS             complement(7545..>7722)
                     /locus_tag="OQM87_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCS1509665.1"
                     /translation="GKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRIAE
                     LEAQTVTVKEVGDA"
BASE COUNT         1779 a         1922 c         1901 g         2120 t
ORIGIN      
        1 actcactatg gcggcgcggc aggtccggtc atctttggcg tgggttacaa cgatattaaa
       61 tcctggtggt tggtcggtgc ggtactgacg atattaacct tcttggtgca tatcaccctc
      121 ggcgtgtggt ggtggaatat gctgatcggc tggaacatgc tgtaaatata ctcgtcatac
      181 ttcaagttgc atgtgctgcg tctgcgttcg ctcaccccag tcacttactt atgtaagctc
      241 ctggggattc actcacttgt cgccttcctg caactcgaat tatttagagt atatccattt
      301 attatctttc tgcgcacttc acggtgcgca gatatttgga gcatttgatg atcaagttat
      361 ctgaaaaagg cgtgtttctc gccagtaata acgaaataat tgccgaagaa catttcaccg
      421 gcgaaattaa aaaagaagaa gcaaaaaaag gcactattgc ctggtctatt ctctcttctc
      481 ataatacgtc cggaaatatg gataaactta aaattaagtt tgattcatta gcctctcacg
      541 atattacctt tgttggtatt gtacagaccg ctaaagcgtc cggaatggaa cgtttcccgc
      601 tgccgtatgt gctgaccaac tgccataact cactctgcgc cgtcggcggc accattaacg
      661 gtgatgacca tgtttttggt ttatcggcag ctcagcgtta tggcggtatt tttgtgcctc
      721 cgcatattgc ggtcatccat caatatatgc gtgagatgat ggctggcggg ggcaaaatga
      781 tcctcgggtc agacagccac acccgttacg gtgcattagg gacaatggca gtcggtgagg
      841 gtggcggtga gttggtaaaa cagctgctta atgacacctg ggatatcgac tatccgggcg
      901 tggttgcggt gcatctgacc ggaaaacctg cgccgtatgt ggggccacag gatgtggcgc
      961 tggctatcat tggcgcggtg ttcaaaaacg gttacgtcaa aaacaaagtc atggagttcg
     1021 ttggaccggg cgttagcgcg ctctctaccg atttccgtaa cagcgttgac gtgatgacca
     1081 cggaaaccac ctgtttaagc tccgtctggc aaactgatga agaagtgcat aactggctgg
     1141 cgctgcacgg tcgcggccag gattactgcc agcttaaccc tcaaccgatg gcgtactacg
     1201 atggctgcat cagcgttgat ttaagcgcca tcaaaccaat gattgcgctg ccgttccacc
     1261 cgagcaacgt gtatgaaatc gacacactga accagaacct gaccgacatt ctgcgtgaga
     1321 ttgaaattga gtccgaacgc gtggcgcacg gtaaagccaa actctcgctg ctggataaag
     1381 tggaaaatgg tcgcctgaaa gtgcagcagg ggattatcgc gggctgttct ggcggtaact
     1441 acgaaaacgt catcgcggcg gcgaatgcac tgcgcggtca atcctgtggc aatgacacct
     1501 tctcgctggc ggtttacccg tcatcacagc cggtgtttat ggatctagcc aaaaaaggtg
     1561 tggtagcaga tttgattggc gcaggcgcaa tcatcagaac cgcattctgt ggcccatgct
     1621 ttggcgcggg cgatacgcca atcaacaacg gtttgagtat tcgccacacc acgcgtaact
     1681 tcccgaaccg cgaaggctct aagccagcca atgggcagat gtcagcggtg gcgctgatgg
     1741 acgctcgttc tatcgctgcg actgcggcaa acggtggcta tttaacctct gccagcgaac
     1801 ttgattgctg ggacaacgtg ccggagtacg ccttcgatgt aacgccgtat aaaaaccgtg
     1861 tttatcaggg ctttgtgaaa ggggcaactc agcaaccgct gatttacgga ccgaacatta
     1921 aagactggcc ggaattgggt gcgctgactg acaatatcgt cctgaaagtg tgctcgaaga
     1981 tcctcgacga agtcaccacc accgacgaac tgattccttc cggtgaaacc tcttcttatc
     2041 gttcaaatcc gattggtctg gcggggaatg tcagcgagct gacagaggtg tttgcgcgca
     2101 ttaagcagat tgctggtcag gagcatattg atccgctgca aactgaaatt ggcagcatgg
     2161 tctatgcggt gaaaccaggc gatggttctg cgcgtgaaca ggcggcgagc tgccagcgtg
     2221 tgattggcgg tctggcgaat attgccgagg agtacgcgac taaacgctac cgttctaacg
     2281 tcatcaactg ggggatgtta ccgctgcaga tggcggaagt gccaaccttt gaagtggggg
     2341 attacattta catccctggc attaaagcgg cgctggataa tccgggtacg acgtttaaag
     2401 gttatgtgat ccatgaagat gcgccggtaa cggaaattac gctttacatg gaaagcctga
     2461 cggctgaaga gcgcgagatt atcaaggcgg gtagtttgat taacttcaat aaaaaccatc
     2521 agatgtaaaa agcgccatgt gaatgtaggt cgcattcggc acttattgtc ggatgcgatg
     2581 ctggcgcatc ttatccgacc tacaaatcgc atcgaaccgt aggccggata aggcgtttac
     2641 gccgcatccg gcaaatagtt aatagctctt acttcttcgc ctctgcaacc actttgctgc
     2701 ccacgccgcg gttattgtat tcccacatgc ggttgtagtt agtgtcattc agattgcgct
     2761 gtatttcgtc gttatcatct acgctgccgg tattacccgc aaacggacga ttagagatca
     2821 ccgcatcggc ccacggttta gccgtgttaa aaccttcgtt gatggcgcta tcacggatca
     2881 ccacctgacc gttggtatta gcatcaacat ccagcgagcg gcccagttgc gccacgccat
     2941 caccggaagc attgaaacgg ctgtttacgg cgaggaaacc gtaatagatg ttagacagcg
     3001 tagccggtgc aaacacatat gcttcttgct gggtacgtga gttcaccacg cggaattcgg
     3061 tgttatcgaa caccactgcg ccgcgaccag aaacgatatc cacatcccct tcaatatagc
     3121 tgttggtcac cagcgtacgc ggctgacggt tggtttccag acggttctgc acaccgctgt
     3181 tggtgacaaa gaaggtgttc tgacgaccga gaatgttaac gttgttaatc tgtacctggt
     3241 caccgtcagt acgcagtgct accgccggat ggttacctgc atctacacta tcgcccagcg
     3301 tgttttcgat ggtcagattt tgcagttgca gtccattgtt ttgtgaccag aagaccgcag
     3361 aacacagaac accgatactg tcgctgcgtt tgctctggca gctatcgtac atataccacg
     3421 ctggtttacc tggcatatat ttgccgcgcg ggttgacgtc gtgacgccag tcggcagggc
     3481 tcatgccccc atcaagggaa agcccaatct tcacatcaat cggtttttca cctgtaccgt
     3541 acagagtgat tccacctgga gcagcaggga catacaccgt tccctgatac tcaccaggca
     3601 tcacggcaat atactggcgc ttgttggtgc gcttgataat tgccgcatct accgccgcct
     3661 gaatcgtggt atgcgttaca ccttgagtac ccgccgggcc gacaacaaag tcaggttgcg
     3721 caggcagggt tatcggggac ggattccacg ctgcagcacc tggtgtcagg gatgcaaaat
     3781 agtgttgcgc atcgaaattc agcgcttctt ttgccgacag aatcgggcga gaagaggtac
     3841 caggcgcagt ttgatcagaa ggacgttgat cgggtggtgt tgagctacag gcggtcagcg
     3901 tcacgccaaa agccaatgcc agcgccagac gggaaactga aaatgtgttc acaggttgct
     3961 ccgggctatg aaatagaaaa atgaatccgt tgaagcctgc ttttttatac taagttggca
     4021 ttataaaaaa gcattgctta tcaatttgtt gcaacgaaca ggtcattatc agtcaaaata
     4081 aaatcattat ttgatttcaa ttttgtccca ctccctgcct ctgtcatcac gatactgtga
     4141 tgccatggtg tccgacttgt gcccgagaag atgttgagca aacttatcgc ttatctgctt
     4201 ctcatagagt cttgcagaca aactgcgcaa ctcgtgaaag gtaggtggat ccccttcgaa
     4261 ggaaagacct gatgcttttc gtgcgcgcat aaaatacctt gatactgtgc cggatgaaag
     4321 cggttcacga cgagtagatg caattatggt ttctccgcca agaatctctt tgcatttatc
     4381 aagtgtttcc ttcattgata tcccgagagc atcaacatgc aatgttgtag ggatggcaat
     4441 ttttacgcct gttttgcttt gctcgacata aagatatcca tctacgatat cagaccactt
     4501 catttcgcat aaatcaccaa ctcgttgccc ggtaacaaca gccagttcca ttgcaagtct
     4561 gagccaacat ggtgatgatt ctgctgcttg ataaattttc aggtattcgt cagccgtaag
     4621 tcttgatctc cttacctctg attttgctgc gcgagtggca gcgaccgggt ttgttgttat
     4681 atggccttca gctattgcct ctcggaatgc atcgctcagt gttgatctga ttaacttggc
     4741 tgatgccgcc ttgccctcgt ctatgtatcc attgagcatt gccgcaattt cttttgtggt
     4801 gatgtcttca agtggagcat caggcagccc cctccttatt gctttaattt tgctcatgta
     4861 atttatgagt gtcttctgct tgattcctct gctggcgagg attttttcgt agcgatcaag
     4921 ccatgaatgt aacgtaacag aattatcact gttgattctc gctgtcagag gcttgtgttt
     4981 gtgtcctgaa aataactcaa tgttggcctg tatagcttca gtgattgcga ttcgcctgtc
     5041 tcggcctaat ccaaactctt tacccgtcct tgggtccctg tagcagtaat atccattgtt
     5101 tcttatataa aggttagggg gtaaatcccg gcgctcatga cttcgccttc ttcccatttc
     5161 tgatcctctt caaaaggcta cctgttactg gtcgatttaa gtcaaccttt accgctgatt
     5221 cgtggaacaa atactctctt ccatccttaa ccggaggagg gaatatcctg cactcgcgta
     5281 cccatcgacg aactgtttca aggctccttg ggcgtcgctg gcgtgcgttc cactcctgaa
     5341 gtgtcaagta catcgcaaag tctccgcaat tactcgcaag ggagcgagta ttggtttatt
     5401 ttctccaaca aaaaaggagc cgaagctcct ttgatgatta aaattcgaat tgtctcgccc
     5461 gaaggctttt cagcattggt tttgcccgtt cgaaaagggc gcttgcttgg tcaagtcgtg
     5521 tcgcctccct tagcagtaca tctctgtttt tcgtcaccgt gtaaaaagtt tcaaacgcga
     5581 tgtcatacag cttgctcgtg tatgatgagt tcagttcctt caatatcggg tacagacgtt
     5641 tgcagatgtc ctgcgcattc tccatctgta cctgcatata gcaaaggagg atgatttctt
     5701 cgtctgtgaa ttgctgcgct ggttgcatgc tgcgaagttt cttttcgcac tcgatgaagt
     5761 atcggcgtat ctggcggcct ttttcgttac gctcgaccat cgccgtttct ttggctgtat
     5821 cgagggtgag gtgatagtct ttgctacggc gatcgcctcc acgtcctttg atccccctgt
     5881 tttggtgaac caaaatgtaa tcgacatcaa cggcaaagcc gtattctgtt atgcgatttt
     5941 taatccatgt agagaagtca cgacgtactt ccaaaaattc gtgtaaatca cgggcattaa
     6001 cgagaagagt tgtttcgttg gatatagtgc cgttgaatac ggggatgagt tgactggtca
     6061 tttttatgtc ctttgcattt gttcagatag ccctgtgttc agcagggcgg tcaggtactt
     6121 gaacaccgtg caaagtcggc ccgtatcctt aacctttcgg ctgtttttcg gtatacgcgc
     6181 tacccgacca tatctgaaaa atggacataa aaaatccgca tgactgacgg gtgcggtttc
     6241 cgctttgcaa ggtgtgttca gcaccatgaa gcggaatata gccccgttaa tgcggtgttg
     6301 tcaaatcatg taggccttat cttgctgtaa gccgcgccat tcgggctttt ccccacattt
     6361 ggggaaaact atcttgtcaa aatcatgcag cctttcgctc tccttcgatg agttcctcta
     6421 ttttgtgcac tccagcctcg ttgtaccgaa atgtttcaac ctgcttgtca ttgtgcgccg
     6481 atttgtcgat gaaccacttc ccgtactttt ctgttttgag catgtaggtg ttggctatgc
     6541 gtcctacctt gttggctgaa ataccgtagc gctggccgac ttccgtagca gagtagtagt
     6601 gctctgagat agctggcaga ggtacaacat cgcgaccaac aatcgggttg aacactccag
     6661 ctactgctgc ctgctttgat tctgaggaca gattcgggaa catattcacc agtcgatccg
     6721 ccacgttcaa agctatttcc atggctcgcg cacggtctag gctactttct ggttgcggta
     6781 ctggttgagg atggttggca accttttcgc gctgattgaa gtagccgtcc tccagttttt
     6841 cgaacacgtc ccatgcctga tccgtttcga gcatcttcgc gtgacgggcg gctccgcgct
     6901 ctgtccagag gatgaggtgg cgtgcatttg gggcactttt cacaaagtag tttaaagcta
     6961 ctttgtgttt tagcgcgcgc agctcgtcac caacgacctt gtagaagtgt accccctcaa
     7021 caaagcgcga cttgttttca tgatggtttt gccggatgcg cacaacatcc gttccgtaca
     7081 actgcgccaa tgtttggtgg gtagttgttt ttaaagtcag ctgctaacac atagcattgt
     7141 cgctctccca tttctggcat tcgctcacta cagcttatcc aggcatccgg agttaccggc
     7201 actggctggg cggtataaag cggtgttata tctgcccgaa aattacatgc tttatgcagc
     7261 cgcacccacc gttcgacttc tgctttgtca gaatacatac cagtgaacgt gttatattca
     7321 cggtcaattt gcgtgaaggt taccttccac gccaccggct ctgctgtcag cgatgccaga
     7381 gcaatttcat aagcacggcg ttcaacattg tctcgcacat ccaaactgct gatacgctct
     7441 ttgatttctt taatcagttc tttgtcgttc acttatgcct ccgattcccg gatgtcaccc
     7501 aaatcaacgc tacaatgcca accagtgcaa cgagcgcaaa actatcaagc atctccaacc
     7561 tctttaacgg taactgtctg tgcctccagt tctgctatgc gcttttctga ggcttcaagt
     7621 aacgcctgct tatcgcgtag cgcttcttcc agttcagcaa catggcattc actatcaata
     7681 aggttgttct ctgctgcttc cagctcaaca cgcaacttcc ct
//
LOCUS       DAJVQH010000158         7518 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJVQH010000158 DAJVQH010000000
VERSION     DAJVQH010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.158"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            215..291
                     /locus_tag="OQM87_003691"
     tRNA            215..291
                     /locus_tag="OQM87_003691"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:249..251,aa:Arg,seq:tct)
     gene            complement(337..1098)
                     /gene="envY"
                     /locus_tag="OQM87_003692"
     CDS             complement(337..1098)
                     /gene="envY"
                     /locus_tag="OQM87_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCS1509666.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINYYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1281..2171)
                     /locus_tag="OQM87_003693"
     CDS             complement(1281..2171)
                     /locus_tag="OQM87_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCS1509667.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2172..5150)
                     /gene="nfrA"
                     /locus_tag="OQM87_003694"
     CDS             complement(2172..5150)
                     /gene="nfrA"
                     /locus_tag="OQM87_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCS1509668.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYIPAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDP
                     ALIRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVG
                     RRWTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLS
                     VYSRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTML
                     RASASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANG
                     QTIEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFK
                     AINQRNGERNNAFLTIGVHW"
     gene            complement(5137..7374)
                     /gene="nrfB"
                     /locus_tag="OQM87_003695"
     CDS             complement(5137..7374)
                     /gene="nrfB"
                     /locus_tag="OQM87_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCS1509669.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVISNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHGAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQAQAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
BASE COUNT         1753 a         1949 c         1963 g         1853 t
ORIGIN      
        1 tttccgttag ggcggtaaat tgtactttga tacatgaaaa tacgggtttt cttgattcag
       61 acgcgcagcg gtgtgcgttt gtttgccgct atagcgaaat aaatcagaaa atcagacgcg
      121 gtcgttcact tgttcagcaa ccagaccaaa agccattgac tcagcaaggg ttgaccgtat
      181 aattcacgcg attacaccgc attgcggtat caacgcgccc ttagctcagt tggatagagc
      241 aacgaccttc taagtcgtgg gccgcaggtt cgaatcctgc agggcgcgcc atttcttctt
      301 catttatgcc cgtcttatcc gtttccgctt tgcccttcac cacatcactt tttgtcgctg
      361 tttggcgaga taattcaacg gtgtcaggct gtaaaacgcc ttaaaaacag agataaagta
      421 cgacgtgctg ctatagccac ataattgcgc cacctgagtg atatttttgt tatccatcaa
      481 taacatctgt acggcgtaac gcatacgaca ctctgtgaca atctggctat agctggtatt
      541 ttcgtttttt aatttctttt tgagcaggct ggggcttaaa catagcgaac tggcgacaat
      601 tcgcagattc cagtaatgct gaatatcgct ttgaataatg cggcagacgc tgtcgcggac
      661 gctgctgcgt aagatataca tcagtagggc aataaaccgc gattgctcaa gaaagttaga
      721 caatacggta aaaagtaatg cgcgcgtcaa ctccgcttcg cagggtttgc tgggttgctg
      781 cttgctgtgg ttggcggcta gccggaacac ttccggtgta cggctatgac aagcgataac
      841 cggggttgcc agccgcgacc agggctttac acaggttaat ttcttattta aaaacaacag
      901 ataatagttg atgatgttac ggttgatgtg ggtgattaat acgtgattta acgttgagag
      961 ttcaatgacg ttattgttgc acgcgaaggc ggccaggtag tttttcggaa gggtaaacgt
     1021 agcatggtta ttgacgctta cctctacctc tttttcggtc aggatcacca cgcaaggttc
     1081 actgctgctc aattgcattt cgcactcctc agatatcaga aactccgctc aaaggatcta
     1141 tgcttcctgc atgagtgatc ggcccgttcg ccgataacga tcttctttct ttagcatgct
     1201 ttttagcaat taatcttgat ggaattctga cgagagcgaa agaggtaagc caggtcgtac
     1261 ccgacttacc tggaggggat ttaatactcg agaatgccgt gcgcgacggg caaatagcgc
     1321 agagagaaat agagcatgtc tttaccgcaa gaggaacgtt tcgctaacag cgaggcaatt
     1381 tctgcgtccg gtttcggttt cgctgtaaag gttttgcctt tgccggcgac aaacagttca
     1441 taaacaatgc cgcttgctgg ggcggagctt tggcaatcgg cgctggcctg tagataacgt
     1501 tcacgctgtt cagcggtaag tttatccacg ccgctgccat cctgtaccca gacattaact
     1561 ccggttgctt taacttgttc cagcagttgg cgatagccat cgggcgacat gtttccggcg
     1621 aaaaaactac tgatataaac cggttttgct gaaacatcgc taatcagccg ctgcgcgttg
     1681 tttaaccatg ttagcaaagg ttgacgggcg gcttcgctgc gccagttcag gtcgtcaatt
     1741 tccgcgctga tgtaccagcc atccggcgtt atgccaggcg cggcgctcca taatctggct
     1801 tgctggagat cggcagccag caggcgatta agatagcttt ccagcgctgc agacgactgt
     1861 ttctggtgca taaaaaattc cggatcggcg ttcagcccga caatcagctt caggccagtc
     1921 tgttgcgcag ctgcggcacg cttaaacaat aacgtgcgct gttctggctg ggtaaatgca
     1981 tcgccgtaac gggtccattg caaaaccagg gtatcgaagc cttgaaaacg taactgactc
     2041 atcagcccct gccactgggt atcggtaacc tgactatctc ggttttgtgg ttgccagata
     2101 atacctttca tcgcaaagga aaaagggctg accaaaagca gtgtcagcaa tacgaaaatg
     2161 aacttacgca tttaccagtg cactccaatg gtgagaaacg cgttgttgcg ctctccgtta
     2221 cgttgattaa tcgccttaaa ggtatgttga tactcgacgc cgagactgac tttgtgcggc
     2281 caggcgtcgt agtgcgtctc gccggtccaa atattccagc ggaccccgac accgccaagc
     2341 tgcgcgccct gagtgccttt atcacgatag ccgttgtcct gaacgtgagc gtaaggctca
     2401 atagtctgtc cgttagctac cttctgatgc cagctgacgc gataatctgc cgtccacgcc
     2461 tgaatatcct ggcggatata ttgcgccgca tcgaggtaca ggttttgggc aaaccagcct
     2521 gaaccgttcg ggtgccattc gtcgctgtat ttgccgccat taaagaatga ggcgctggcg
     2581 cgcagcatgg tatcggatgc gccattttgc ccgttcagcg gcaactgctg ttcgacggcg
     2641 aggaaaaaga tctgatcgcg cagcggcttc cagcgcagac cggtgccgga catcggattt
     2701 ttcaccggca tcatcacccc gttttctccg gtatcggcaa agacgcggct ataaactgag
     2761 agcaggtcgc cttccagcag catattgcgt ccgatgcggt actcggcttc cagttgtccg
     2821 tagctacgat agcttttccc aggcgctgca ccgccgacat tattgttagc ggtactcatt
     2881 gcgccggaac gcaagccgat ggaagaatcg aaactgaacg tccagcggcg accgacctcc
     2941 tcatgcaaac ggcggaaatt gaagcgttgc tgattttgct ctggggtcag tggggttatc
     3001 agcgcctgat tatcaatgtc atcaatcacc agccgggcgt agtgctgcgt cgcaggcatg
     3061 tcatccagac gctggttcac gtaggccagt tgtcggatca gtgccggatc gtccggaagc
     3121 cctttatgcg cctgttcgag catttcccgc gactgtgcga tatcaccgct atcccacaag
     3181 gcgtaaccaa gcgctgcctg ggtgttgcta ttattcggtt ccagttccag ttccagcgcg
     3241 gcgcgcaaat cactcaccgc ggccggaaca ttatgacgtt ggcgataaat tgtcgcccgc
     3301 gcaacgtaag cgttggcaga aggggcaata ttgattgagc gcgtgagatc gttcagtgcg
     3361 agttctggct gagctgggat ataacgttgc gcatgcagcc accagtattg agcattgttt
     3421 cccagctcac gttgttctgc ctgttgaagc cagcgatcgc gagccgcgcc atttcccgct
     3481 gcctgagcgg tgttggcggc ggcgagcaga tcctcattgc tcatgtcgtg aagactgatt
     3541 ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg ataggctacc
     3601 gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc atacaacgcc
     3661 acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc agcatcgtag
     3721 gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc aatacccggc
     3781 aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa aatcgccacc
     3841 ttcgccggcg ttgctaggta aggatgactt tccagcagag acgccagtcg cgccattaaa
     3901 gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa atcggcagct
     3961 tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg gggaagattt
     4021 gtcggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt gttacgggtc
     4081 gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt ggcgagcagt
     4141 ttttgcgctg cgtcgtactg accctctttt aacagcaccg gtagcgtcgc gccaacaaca
     4201 tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac ggggttggcg
     4261 ctgtatttag ataacagata gagccaactt ttctcttgtg cgcccgtggt aaacagcggc
     4321 ttattttcaa tgagataatg ctggaggcgt gctttttcgc cacgataagc cagcgcggtc
     4381 gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag tgccaggatc
     4441 cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc tgccgcgctt
     4501 aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca ttgtttcagg
     4561 tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga tgcagcaaac
     4621 gtcgcatcgt tcagttgcgc tctggcgaca ggtaattgtg ccagccgcag ggcattctgc
     4681 ccgacttcac tgcgacaacg cagggtcggc gcagcgtcgc ccgctttttg ctgggcaagc
     4741 agttcttcaa cagtcgtaac gcttttcact tcaactggaa tagccgccag actgcgctca
     4801 agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg cgcccggtca
     4861 tcatgaccaa aatgacgata ggcttccgca aggtataaag tcagcggaat attatccggc
     4921 acctgttggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt atttttctgt
     4981 gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc gctcagcccc
     5041 agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa taaagacgtc
     5101 agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat tttcggactc
     5161 cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt gcatcgaaac
     5221 ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga taacgccttc
     5281 ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata acaacacgtt
     5341 aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca ggaactgatg
     5401 cggcacatat tgccgccaga tttcaccggt ctgctgttcc gtgagccact gatgctgaac
     5461 cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc agtggcgtaa
     5521 ccctgtgaca atttgtcccc gcagaacaat gacgtaacgc actttgcgtc cgactttacg
     5581 cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa ttaactcatc
     5641 attttccaga cgcagcggca gtactgcata atgcagcgct acggaggctg gcatttcggc
     5701 aatcagcgag gacgggatct gccaggcatc gatggattcc cacgccacgc cgttttgctc
     5761 tgcctgcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca ttgaaccgcc
     5821 caggcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgttctt cagtgatgac
     5881 ctgattttcc agcagaattt gacctaacgg gcgcaacgag cgggtatcgc cagtgacgct
     5941 ggggaagtca tgcgttgttt tatcccacgc cacgcgacgc ggatcgccgt gctgaagaac
     6001 ctgtttcagc acgcgccagt tagccataaa gttaatcagg ttgccccaga aaagacgcag
     6061 gacggaaagc aggccctgcg tgaggccgta gtagccagtg acgaaaatca ctcgctgcac
     6121 gatgcggtta accatcaagc caaagtttag ccacagcagg gtcattaacc atgcgctgcc
     6181 gctaaaaata gaaaggaaat gccaggcatc gggccacaaa ctttcatacg ccagcaacag
     6241 caaaagctgg atcatcacca gcatcgcgag gaagctgaca aagttactga ttgccccttt
     6301 gcggtcgcgc cagagaaagt agttcagcgt caggctggag gtccatttat gggttttaaa
     6361 gccctggaaa acaatgccga tgatccagcg agatttttgt cgaaccgcag tcgaaaaggt
     6421 atcggggaaa tattcgcgca cgcagatcat gtttgacgtc cgtgcgtgct gtaaaaattt
     6481 acgctgctcg cgttctttgg cttcgtccac caccggaaaa cggacaaaaa tttccgtcat
     6541 acctttttct ttcaggcgga agccaatgtc gtaatcttca gtaagactct gcacgtcgaa
     6601 agcaataccg ttaccgtcag ctaacagtgc ggtcacggcg cggcggctga aacaggtgcc
     6661 gacgcctgcg ctgggcactt gtccggcgag ggcttcacgc accggaacat ctttgccatg
     6721 cagttctgaa aactcatcaa tgtaagtcat gctggtgaag tgcgtccatt cgcgttcaaa
     6781 cggatacact gggatctgaa tcagatcttt acgctcgacc agatagttga acagacgcaa
     6841 ttccatcggt gaaatcacat cttcggcgcc atgcagaata aaaccagcaa aagcgaaatt
     6901 ggcgctacgt tcaaactggg tgatggcgtc cagcacgttg ttcagacagt cggctttgct
     6961 ggtgggacca ggacgcgcgc agactacctt atgcacgttc ggaaagcgag cgcacacttc
     7021 gtcaacatca cgttgagtat cggggtcgtt ggggtaggtg ccaacaaaga tatgatagtt
     7081 ttcgtagtcg agcgtggtcg ccgccagctc ggccatattg ctgatgacgc ccgtttcatt
     7141 ccacgccgga accataatcg ctaacggttt ttcatctggt ttatacagtt cgcggtaact
     7201 cattcgcggg tagcggcgat aaacactcaa cttgcgttta atgcggcgta cccagtagac
     7261 gacatcaata aaaaaatcgt ccagcccgct gatgaacatg atgaccgcta acgttatcgc
     7321 gattactttt aagccgtaga gccaggtagc aaaaacatca agaagccagt ccacacaaaa
     7381 accttacatt aacgctggtt atgtttaggg tggcgtatat taaggttttt tatgaattgt
     7441 gacagctttt taccattaat agggtaatga ctccaactta ctgatagtgt tttatgttca
     7501 gataatgccc gatgacct
//
LOCUS       DAJVQH010000159         7508 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJVQH010000159 DAJVQH010000000
VERSION     DAJVQH010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.159"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..1153
                     /gene="rbsA"
                     /locus_tag="OQM87_003696"
     CDS             <1..1153
                     /gene="rbsA"
                     /locus_tag="OQM87_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCS1509670.1"
                     /translation="YVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKV
                     IIMDEPTDALTDTETESLFRVIRELKSQGHGIVYISHRMKEIFEICDDVTVFRDGQFI
                     AEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKG
                     EILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDDLANGIVYISEDR
                     KRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIG
                     LLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVS
                     SEMPEVLGMSDRIIVMHEGHLCGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            1158..2117
                     /gene="rbsC"
                     /locus_tag="OQM87_003697"
     CDS             1158..2117
                     /gene="rbsC"
                     /locus_tag="OQM87_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCS1509671.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIVGT
                     LIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2142..3019
                     /gene="rbsB"
                     /locus_tag="OQM87_003698"
                     /pseudo
     CDS             2142..3019
                     /gene="rbsB"
                     /locus_tag="OQM87_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3145..4074
                     /gene="rbsK"
                     /locus_tag="OQM87_003699"
     CDS             3145..4074
                     /gene="rbsK"
                     /locus_tag="OQM87_003699"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS1509672.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQLLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4078..5070
                     /gene="rbsR"
                     /locus_tag="OQM87_003700"
     CDS             4078..5070
                     /gene="rbsR"
                     /locus_tag="OQM87_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCS1509673.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5036..6463)
                     /gene="mdtD"
                     /locus_tag="OQM87_003701"
     CDS             complement(5036..6463)
                     /gene="mdtD"
                     /locus_tag="OQM87_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCS1509674.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVTRLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6486..7178)
                     /locus_tag="OQM87_003702"
     CDS             complement(6486..7178)
                     /locus_tag="OQM87_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCS1509675.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1909 a         1816 c         1979 g         1804 t
ORIGIN      
        1 gtatgtcgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt
       61 agtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
      121 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
      181 atccctgttc cgcgtcatcc gcgagctgaa atcgcaaggc cacggtattg tctatatctc
      241 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca
      301 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
      361 gggccgcaag ctggaagatc aatatccaca cctggacaaa gcgccaggag atatccgcct
      421 gaaagtcgat aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg
      481 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
      541 ctacggcgca ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
      601 ccgttcaccg caggatgacc tggcaaacgg cattgtgtat atctccgaag accgtaaacg
      661 tgacggttta gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
      721 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
      781 cattcgtctg tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg
      841 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtattgat
      901 ccttgatgag cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
      961 taaccagttc aaagccgatg gcttgagtat cattctggtg tcatcggaga tgccagaagt
     1021 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctctgcg gggaatttac
     1081 tcgtgagcag gccacccagg aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt
     1141 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
     1201 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
     1261 cgttaagccc gaactttttc accatcaata acttattcaa tattctccag caaacttcgg
     1321 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atagacttgt
     1381 cggtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag
     1441 tcaatgcgct ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt ggtgcggtaa
     1501 ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc
     1561 ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
     1621 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
     1681 cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc
     1741 tggggcgtta catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca
     1801 acgtcaataa aatcatcgtc tattctcttt gtggtctgct ggcatcgctg gccgggatca
     1861 ttgaagtggc gcgtctctcc tctgcacaac ccacggcggg gactggctat gagctggatg
     1921 ctattgctgc ggtggttctg ggcggtacga gtctggcggg cggaaaaggt cgcattgttg
     1981 ggacgttgat cggcgcatta attcttggct tccttaataa tggattgaat ttgttaggtg
     2041 tttcctccta ttaccagatg atcgtcaaag cggtggtgat tttgctggcg gtgctggtag
     2101 acaacaaaaa gcagtaataa cgactacagg acatcttgaa tatgaacatg aaaaaactgg
     2161 ctaccctggt ttccgctgtt gcgctaagcg ccaccgtcag tgcgaatgcg atggcaaaag
     2221 acaccatcgc gctggtggtc tccacgctta acaacccgtt ctttgtatcg ctgaaagatg
     2281 gcgcgcagaa agaggcggat aaacttggct ataacctggt ggtgctggac tcccagaaca
     2341 acccggcgaa agagctggcg aacgtgcagg acttaaccgt tcgcggcacc aaaattctgc
     2401 tgattaaccc gaccgactcc gacgcagtgg gtaatgctgt gaagatggct aaccaggcga
     2461 acattccggt tatcactctt gaccgccagg caacgaaagg tgaagtggtg agccacattg
     2521 catctgataa cgtactgggc ggcaaaatcg ccggtgatta catcgcgaag aaagcgggtg
     2581 aaggtgccaa agttatcgag ctgcaaggca ttgccggtac atccgcagcc cgtgaacgtg
     2641 gcgaaggctt ccagcaggcc gttgctgctc acaagtttaa tgttcttgcc agccagcaga
     2701 ttttgatcgc accaaaggtt tgaacgtaat gcagaacctg ttgaccgctc atccggatgt
     2761 tcaggctgta ttcgcgcaga atgatgaaat ggcgctgggc gcgctgcaaa ctgccggtaa
     2821 atcggatgtg atggtcgtcg gatttgacgg tacaccggat agcgaaaaag cggtgaatga
     2881 tggcaaacta gcagcgacta tcgctcagct acccgatcag attggcgcga aaggcgttga
     2941 aaccgcagat aaagtgctga aaggcgagaa agttcaggct aagtatccgg ttgatctgaa
     3001 actggttgtt aagcagtagt tttaatcagg ttgtatgacc tgatggtgac ataaatacgt
     3061 catcgacaga tgaacgtgta atataaagaa aagcagggca cgcgccaccc taacacggtg
     3121 gcgcatttta tggacatccc gaatatgcaa aacgcaggca gcctcgttgt tcttggcagc
     3181 attaatgctg accacattct taatcttcaa tcttttccta ctccaggcga aaccgtaacc
     3241 ggtaaccact atcaggttgc atttggcggc aaaggcgcga atcaggctgt ggctgctggg
     3301 cgtagcggtg cgaatatcgc gtttattgcc tgtactggcg atgacagcat tggcgagagc
     3361 gttcgccagc tgctcgccac tgataacatc gatattactc cggtcagcgt gatcaaaggc
     3421 gaatcaacag gtgtggcgct gatttttgtt aatggcgaag gtgagaatgt catcggtatt
     3481 catgccggcg ctaatgctgc tctttcccca tcgctggtgg aagcgcaacg tgagcgtatt
     3541 gccaacgcgt cagcgttatt aatgcagctg gaatcaccac tcgaaagtgt gatggcagcg
     3601 gcgaaaatcg cccatcaaaa taagactatc gttgcgctta acccggctcc ggctcgcgaa
     3661 cttcctgacg aactgctggc gctggtggac attattacgc caaacgaaac ggaagcagaa
     3721 aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag cgaaggcggc gcaggtactg
     3781 catgaaaagg gtatccgtac tgtactgatt actttaggaa gtcgtggtgt atgggctagc
     3841 gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc aggctgtcga taccattgct
     3901 gccggagata cctttaacgg cgcgttaatc acggcgttgc tggaagaaaa accattgcca
     3961 gaggcgattc ggtttgccca tgctgccgct gcgattgccg taacacgtaa aggcgcacaa
     4021 ccttccgtac cgtggcgtga agagatcgac gcatttttag acaggcagag gtgacgcttg
     4081 gctacaatga aagatgttgc ccgtttggcg ggcgtttcta cctcaacagt ttctcacgtt
     4141 atcaataaag atcgcttcgt cagtgaagcg attaccgcca aagttgaagc ggcgattaaa
     4201 gaacttaatt acgcgccatc agctctggcg cgtagcctca aactcaatca aacacatacc
     4261 attggcatgt tgatcactgc cagtaccaat cctttctatt cagaactggt gcgtggcgtt
     4321 gaacgcagct gcttcgaacg cggttatagt ctcgtccttt gcaataccga aggcgatgaa
     4381 cagcggatga atcgcaatct ggaaacgctg atgcaaaaac gcgttgatgg cttactgtta
     4441 ctgtgtaccg aaacgcatca gccttcgcgt gaaatcatgc aacgttatcc gacagtgcct
     4501 accgtgatga tggactgggc tccgttcgat ggcgacagcg atcttattca ggataactcg
     4561 ttgctgggcg gagacttagc aacgcaatat ctgatcgata aaggtcatac ccgtatcgcc
     4621 tgtattaccg gtccgctgga taaaactccg gcgcgcctgc ggttggaagg ttatcgggcg
     4681 gcgatgaaac gtgcgggtct caacattcct gatggctatg aagtcactgg tgattttgaa
     4741 tttaacggcg ggtttgacgc catgcgccaa ctgttatcac atccgctgcg tcctcaggcc
     4801 gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc aggcgttata tcaggcagag
     4861 ttacaggttc cgcaggatat cgcggtgatt ggctatgacg atatcgaact ggcaagcttt
     4921 atgacgccac cattaaccac tatccaccaa ccgaaagatg aactggggga gctggcgatt
     4981 gatgtactca tccatcggat aacccagccg acccttcagc aacaacgatt acaacttact
     5041 ccgattctga tggaacgcgg ttcggcatag atttacgctg tcttttgatc aaattattac
     5101 catcggttgt tttcagaagc atgaacattg ctgctgaagc aacagtaata atgcccatcg
     5161 tgataaacgt atagtggaat tgttcgacag tcgttgtgcc ttccatccct tcataaatgc
     5221 gaaggacggc cgcacttaca gcaacgccta aactaatcga cagttgctgc gtgaccgcca
     5281 gaacattgtt accgctgctg gcgttgtcat cggtcagatc ggcaagtgtg atggtattca
     5341 tcgcggtaaa ctgcgtcgac atagccatcc ctaatataaa caacggcaag atcagcatcc
     5401 atatcgccat tgccggtgat tgcaaagaga actgagcgat cattagccca ataatcaccg
     5461 tgatccccac taatgtatgg cgatagccca gacgacgtaa gacttgggta accatcgatt
     5521 ttgcaataat ggaacctaac gctgtcggtg ccatcataca gccggcaata aacgcctgat
     5581 aaccaaatcc tacctgtaac atcaatggca taaggaacgg tacacagccg gtccccagac
     5641 gggttgcaat attgcctacg ataccgatcg agaaagtgcg ggttttaaat aaatctaatg
     5701 aaattaatgg gtttggcgta cgtcgcgcat ggagaatata gagaagcagt aacccgatgc
     5761 tggtgacaat taccgtcaag gcaatccagc tggcgacaat cttttccccg aatagctcta
     5821 ttccgcttga aaagagaaca aggctgaggc caaacagcaa aaagccagtg atatcgaatc
     5881 tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgcgcgc gtaaagaagg cccgcaatac
     5941 ctatggggat attaattaaa aatatccagt gccaggttgc ccaggtgacc agcacgccgc
     6001 caagaacggg gcctaaaatt ggccccacca gacccggcat ggcgacaaaa ttcaataccg
     6061 gaagaagttc attacgagga taagcacgca gtaaggccag ccgagtaaca ggcatcatca
     6121 ttgcgtcgcc tatcccttga ataacccgga agacaaccag ctgtggtagc gaattagaaa
     6181 gtgcgcaggc cagagaaccc aacgtgaaca gactcacggc aagggtaaaa atgcgacgcg
     6241 taccgaagcg atcggctagc catccgctta ccggaataag catcgccacc gtcagcgtat
     6301 aactgatgat ggctgattgc atcgcgagag gagaacgatt aaggctatga gcgattgcgg
     6361 gtaaggcggt attcagaata gtggcatcaa gtgcctgcat gaaaaaggcc atcgccgcga
     6421 tccacggcaa acccgacata ctacgcttct ttttatcgct cattcaatgt cctgttatcg
     6481 ggttatcact tatcaggtga gcgtagcagc gcctgacaag ctttaaatgc cgcgtcgcca
     6541 tcgctttgga taatcgcatc gacaatcgcc tggtgcagat ccagctttat cactgtgtcg
     6601 ctggtaattg acgtgaagta agtgtgataa accgaatgga atagcgaggc gaatgaggtc
     6661 aaaaacggat tggcgctcat ttcatagata tgctcatgcc aggctatatc gacttcgatc
     6721 cagcgttcac ggcgaaagtt ctcttttaat gccgccattt cggccattaa cgtattgaga
     6781 tgcgccttct gttccgcggt gccaaccgtt gctgccagta ggcaggcttg cggctccaga
     6841 cagatacgca taaccagaaa gtgatcgatg acctgatgaa agttctcttc tgtcatccac
     6901 caagtaagca attcctgatc aagaaaattc cagttcgatt gtggcatgac ccgagtacca
     6961 attcgcggtc gcggtaaaac catccctttt gccgttaacg ttttgaccgc ttcgcgtacc
     7021 gctgtacgac tcactccaaa ttgctcgccc agctcaatct caccgggcaa aatggtgccg
     7081 ggttcatatt caccttttaa gatccgttgc gccagcttct cagccagaac atacgaaagg
     7141 tttttctgtg cagctaactg ttgtgcgctt aaaggcatta cttatcttcc tttttctttt
     7201 tattcctcct tagtatgcca ccaggaagtg tgattacggt tgcaaaaacg gcaaattgat
     7261 tgttttatgg cacattaacg gggcttttgc tgaaaaaatg cgcggtcaga aaattatttt
     7321 aaatttcctc ttgtcaggcc ggaataactc cctataatgc gccaccactg acacggaaca
     7381 acggcaaaca cgccgccggg tcagcgggat tctcctgaga attccgtcag agaaagcaaa
     7441 aataaatgct tgactctgta gcgggaaagc gtattatgca cacctcgcgc cgctgagaaa
     7501 aagcgaag
//
LOCUS       DAJVQH010000160         7449 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJVQH010000160 DAJVQH010000000
VERSION     DAJVQH010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.160"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..223)
                     /locus_tag="OQM87_003703"
     CDS             complement(<1..223)
                     /locus_tag="OQM87_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCS1509676.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSFGFAP"
     gene            743..1438
                     /gene="minC"
                     /locus_tag="OQM87_003704"
     CDS             743..1438
                     /gene="minC"
                     /locus_tag="OQM87_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCS1509677.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1462..2274
                     /gene="minD"
                     /locus_tag="OQM87_003705"
     CDS             1462..2274
                     /gene="minD"
                     /locus_tag="OQM87_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCS1509678.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2278..2544
                     /gene="minE"
                     /locus_tag="OQM87_003706"
     CDS             2278..2544
                     /gene="minE"
                     /locus_tag="OQM87_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCS1509679.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2916..3260)
                     /locus_tag="OQM87_003707"
                     /pseudo
     CDS             complement(2916..3260)
                     /locus_tag="OQM87_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3295..3414)
                     /locus_tag="OQM87_003708"
     CDS             complement(3295..3414)
                     /locus_tag="OQM87_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS1509680.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3375..3560)
                     /locus_tag="OQM87_003709"
     CDS             complement(3375..3560)
                     /locus_tag="OQM87_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509681.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3661..3834
                     /locus_tag="OQM87_003710"
     CDS             3661..3834
                     /locus_tag="OQM87_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509682.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3836..4180
                     /locus_tag="OQM87_003711"
     CDS             3836..4180
                     /locus_tag="OQM87_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCS1509683.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4184..4519
                     /locus_tag="OQM87_003712"
     CDS             4184..4519
                     /locus_tag="OQM87_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCS1509684.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4576..6897)
                     /locus_tag="OQM87_003713"
     CDS             complement(4576..6897)
                     /locus_tag="OQM87_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1509685.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPIPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(7014..7223)
                     /locus_tag="OQM87_003714"
     CDS             complement(7014..7223)
                     /locus_tag="OQM87_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509686.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2043 a         1752 c         1652 g         2002 t
ORIGIN      
        1 taggggcaaa tccaaatgat tgtaagtttt cagcggcttt ttcgccgaga tatttccttg
       61 ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa acaacgccgc
      121 acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag aacatcatcc
      181 ccacacctga caatccgata taaataatac gcatcatatt catagcacat cctgataatc
      241 caaccgtaca acacttatgc tcatttcgtc atcaatccgt aatcaaaacc gctgacctgg
      301 acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta tgatagagca
      361 attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg caaaaaatgg
      421 aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg gatatttaaa
      481 caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa taagaagaat
      541 caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat gaatcagcgc
      601 catttatcac agaatagact tttactctga ataaatggga gggtgacttg cctcaatata
      661 atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg cgatgattaa
      721 tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag gcagtagctt
      781 cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc aggcgctgaa
      841 agacaaaatc gctcaggccc ccacattttt aaaacatgcc cccgttgtac tcaacgtcag
      901 tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg caaccggttt
      961 gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa ttgaaaaaat
     1021 ggggctgcct atcctgacgg aaggaaaaga aaaagcgcca cgtccagctc ccgcaccgca
     1081 ggctccagcg caaaatacaa tgccggtcac aaaaacgcgt ttaatagata ccccggtgcg
     1141 ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc acgttagcgc
     1201 tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc gcggtcgtgc
     1261 gctggcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc tgatggcgga
     1321 attggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag aattttatgg
     1381 caaagcgggg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt taaattgatc
     1441 cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg taaagggggt
     1501 gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa gggaaagaaa
     1561 actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat gggttgcgaa
     1621 cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct aaatcaggcg
     1681 ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca aacacgcgat
     1741 aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa agcgatggat
     1801 tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt aatggcactc
     1861 tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt acgcgactct
     1921 gaccgtattt taggcattct ggcgtcaaaa tcacgccgcg cagaaaatgg cgaagagcct
     1981 attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag aggtgacatg
     2041 ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt gatcccagag
     2101 gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga cattaacgcc
     2161 gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga acgtcctttc
     2221 cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg ataagttatg
     2281 gcattactcg atttctttct ctcgcggaag aagaacacag ccaacattgc aaaagaacgg
     2341 ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta tctgccgcag
     2401 ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc tgagatggta
     2461 accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa cgtgacctta
     2521 ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt caaggcagag
     2581 tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc ggcgagtatt
     2641 cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag gctgttccca
     2701 cgcacagaac aaaatgttta tgcctggttg agtaaataac catgttgtta gtgtggatat
     2761 gctgggagat gataaccccg caatttcatt attcattgtt ctcccatagg atgaggtttc
     2821 ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat cctgtaaacg
     2881 aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt cacaccagcc
     2941 accgcgttcc acggggactc cacaccggca ccatggctat accccacccc gaaatgcccg
     3001 cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg tctgcccgac
     3061 aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc gacaaattct
     3121 ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag acgcatgttg
     3181 taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga tttcgattcc
     3241 atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc aacatcagcc
     3301 cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat tgtgttcacc
     3361 gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag gcatttttta
     3421 cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata atactgtcgt
     3481 acgcttatct ttagacaatg aggccgtcgg caaaataaaa tgactaatgt aatcgttgct
     3541 gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc agattatctt
     3601 acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata ttgtcaatca
     3661 atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat tcctatcatc
     3721 acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt taagcagatc
     3781 ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat ctaagttgaa
     3841 gaaaaaaata cttgccttcg ggcttatctc agcattgttc tgctccacgc cagcgatggc
     3901 ggatatgaac cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt tacttactgg
     3961 caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg gtgcagtaac
     4021 agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtgggtg ccgccgttgg
     4081 tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag gtgccgtgat
     4141 tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga tgaaaaaatt
     4201 tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg actacaatat
     4261 taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg aaatggcaaa
     4321 aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca gcgttgccag
     4381 ccgtgattta aaaattgagc aatctccgga actgagcgcc aaagtggtag aaaaactgaa
     4441 tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg acaccatgcg
     4501 tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga cgcggttttt
     4561 tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg tgtcattgta
     4621 gccattatcg cccagctgca cgcccacatt cccccagacg ctggccgctg gattcaggtt
     4681 gccatttacg ccaagtttaa tttcccccaa attactcacc ccatcttgtt cgactttcac
     4741 accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt caacaaaagg
     4801 ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa aggtgcggaa
     4861 gccgagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc cattggcttc
     4921 cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct ggaagatcca
     4981 cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaaaccgt aacccgcttc
     5041 cagcgagacg gttgcacctt tcgcgctata ggattcatag gctaattcat cccctttcac
     5101 ggagttttta aaccagctat attgcgccca ggagtcaatg tatgcgcctt ttttactgat
     5161 gtcatcggca aaccaagtgg catacagccc tgcgctatag cctctgacac tccctttcga
     5221 acgataatcc gacacgctgg aatgagttaa gttgtagtcg cgggcataac cagccatcac
     5281 tcccaaacgc caactgtcac tatcggtaaa accaccggtt aacagatcgc cccctaactg
     5341 cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc aggcattatg
     5401 cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta caggatcgat
     5461 gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt tagcggctgc
     5521 caggttggcg acgtaacttc cagcttcggg gcgcaggacg gggataggcg caacagtagg
     5581 cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt tagtcagata
     5641 ccagttttta ttggtcgctt cggtattacc tcgggttaat gaatattcgt aggcaccggc
     5701 gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc tgataatctc
     5761 tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa ctcgggttgt
     5821 tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat catccaattc
     5881 agttctgaga tacaatacgc cattattacc aacgtaattt tcagttattg ttaatcttgt
     5941 tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat caaccgtgag
     6001 gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa gcgacagata
     6061 ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg aattagttgc
     6121 atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg atgatacctc
     6181 actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt catttgctga
     6241 ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgactattta tggaagactc
     6301 cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg tcaccgtgcc
     6361 gccattataa attttagcaa tcgcggcgtc aggacccgtt gtataaattt ctccctggtt
     6421 aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat gcgtggtgat
     6481 atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac cagccttgac
     6541 gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga tctccccttt
     6601 tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg cagtactttc
     6661 ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg caagaacgcc
     6721 gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa aagagggggt
     6781 aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc cactcgtttg
     6841 acttagcaaa ccaattgagt aatcccccgt tgtgacaatc gtgtttttac cctgcaaaat
     6901 aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgggc ttagaggact
     6961 attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa tggttaactt
     7021 actaccctct cctgcaacgg atacccgtaa cttccattac ccgttgttat aactagcgca
     7081 ttatcaatat cttttacttt tccatctttt attgccacag catgtttata ttcatcgtgc
     7141 tgaataaact gttcagcata tgcattaccc accgccagag caataagtcc caaactaaac
     7201 ccggggagtt ttttcagttt catagattaa ccatccattc attggattaa caaacattcc
     7261 tggtcaaata acctatgagc tcagattacc aaaacggagg gtaaagacaa aaacaaaagc
     7321 acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag tcggcattat
     7381 catttttcac cttattttca tgacattgat cactttgggg tgactatttt gtttaacaaa
     7441 taactcttc
//
LOCUS       DAJVQH010000161         7367 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJVQH010000161 DAJVQH010000000
VERSION     DAJVQH010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.161"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..979
                     /locus_tag="OQM87_003715"
     CDS             <1..979
                     /locus_tag="OQM87_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1509687.1"
                     /translation="KYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGS
                     GLGIAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGR
                     TVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFL
                     NVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFW
                     ILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVM
                     FIAIDMDKLKQINDNLGHQEGDLAITLHDAYKASDERLYVNKQNKNSRS"
     gene            1022..1525
                     /gene="yeaK"
                     /locus_tag="OQM87_003716"
     CDS             1022..1525
                     /gene="yeaK"
                     /locus_tag="OQM87_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCS1509688.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVIHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1526..1630)
                     /locus_tag="OQM87_003717"
     CDS             complement(1526..1630)
                     /locus_tag="OQM87_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509689.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1800..2246
                     /locus_tag="OQM87_003718"
     CDS             1800..2246
                     /locus_tag="OQM87_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCS1509690.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2203..3024)
                     /gene="nimR"
                     /locus_tag="OQM87_003719"
     CDS             complement(2203..3024)
                     /gene="nimR"
                     /locus_tag="OQM87_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCS1509691.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3121..4302
                     /gene="nimT"
                     /locus_tag="OQM87_003720"
                     /pseudo
     CDS             3121..4302
                     /gene="nimT"
                     /locus_tag="OQM87_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4357..4704
                     /locus_tag="OQM87_003721"
     CDS             4357..4704
                     /locus_tag="OQM87_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCS1509692.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4726..4980)
                     /gene="yoaF"
                     /locus_tag="OQM87_003722"
     CDS             complement(4726..4980)
                     /gene="yoaF"
                     /locus_tag="OQM87_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCS1509693.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5163..6188
                     /gene="dgcP"
                     /locus_tag="OQM87_003723"
     CDS             5163..6188
                     /gene="dgcP"
                     /locus_tag="OQM87_003723"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCS1509694.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6221..6319
                     /locus_tag="OQM87_003724"
     CDS             6221..6319
                     /locus_tag="OQM87_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCS1509695.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6322..6396
                     /gene="yoaJ"
                     /locus_tag="OQM87_003725"
     CDS             6322..6396
                     /gene="yoaJ"
                     /locus_tag="OQM87_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCS1509696.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6448..6879)
                     /locus_tag="OQM87_003726"
     CDS             complement(6448..6879)
                     /locus_tag="OQM87_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509697.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKALEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(6873..7100)
                     /locus_tag="OQM87_003727"
     CDS             complement(6873..7100)
                     /locus_tag="OQM87_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1509698.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
BASE COUNT         1809 a         1763 c         1777 g         2018 t
ORIGIN      
        1 taaatacccg ggcttacgcg gcacactaca aaccgcatcc actgactgcg acacaattgt
       61 ggaagctgca gcactattac ccgcttttga tcaggcagtg gaaggcaacc gccaccagga
      121 tgattacggt tcaggtcttg ggatagccga agagaaattt cactattatc tcgatctcaa
      181 tgaccgctat gtctattttt atgagccggt taatgttgaa tactttgcga tgaataactg
      241 gtccttcctg cagtcaggaa gtattggcat cgatcgcaaa gatattgaaa aggtatttac
      301 cgggcgtacc gtattgtcga gcatttacca ggatcagcgt actaaacaga acgtgatgag
      361 tttgctgacg ccggtatatg tcgcagggca gctaaaaggg attgtgctgc tggatattaa
      421 caaaaacaat ttgcggaata tcttttacac tcatgaccgc cctctcctct ggcgttttct
      481 caatgtcacg ctaaccgata ccgattcggg gcgcgacatt atcatcaacc agagcgaaga
      541 taatctgttc cagtatgtca gttacgtcca tgacttaccg ggcggcattc gtgtctcgtt
      601 atccattgat attctttact ttatcacgtc ttcgtggaaa agcgttctgt tctggatttt
      661 gacggcgtta attttgctga atatggtccg gatgcacttc cgtttatacc aaaatgtgtc
      721 gcgagaaaat attagtgatg cgatgactgg actgtataat cgcaaaattt taacccctga
      781 actggagcag cggttgcaga aactggtgca atccggttct tcggtgatgt ttattgcaat
      841 tgacatggac aagttaaagc aaataaatga caacctcggt catcaggagg gggatttagc
      901 gattacgtta catgatgcgt ataaagcttc cgatgagcgt ttatatgtca ataagcagaa
      961 caaaaacagc cgttcatgat aaccttctgt ggttgtttgc ttgtaatctc aggagcgtga
     1021 aatgactgaa atggctaaag gaagcgtgac gcatcagcag ttaatcgctt tattatcaca
     1081 agaaggtgct aacttccgcg tcgttatcca tgaagcggta ggtaaatgtg aagcggtgtc
     1141 agaaattcgt ggcaccgctc taggccaggg tgctaaggca ctggtatgta aagtcaaagg
     1201 caatggcgta aatcaacatg ttctggcgat cctcgcagcc gatcaacagg ccgatctgag
     1261 ccaactcgcc agccatatag gcggattacg cgcttcgttg gccagcccgg cggaagttga
     1321 tgaactcaca ggctgtgtct tcggcgcgat cccccctttc agcttccatc caaaactcaa
     1381 actggttgcc gaccctctac tcttcgaacg atttgatgaa atcgccttca atgcagggat
     1441 gctggataaa tccgttattt tgaaaaccgc ggattatttg cgcattgcgc aaccagaact
     1501 ggtcaatttc cgccgcactg cgtagctagc ctatccgttc aataagcaga acggacaaaa
     1561 ccagcacaac cgcaatagca aaaaacgatg atgtgataat cagtgtctcg acgaacattt
     1621 gatcgttcat agcatgcccc cgggagatag ctgttttcac gttattgtta gcgtgcacaa
     1681 atggcagttt gatgacagtt cgcctgattt tttatgcaaa aaacgtaaag attttctact
     1741 tccttcctgc agcaagcgta aagtaagcag gcttattatt ttttggcaag gaaaccacga
     1801 tgtttgatgt cactttgctg atcctgctcg ggttagctgc gctgggcttt atcagtcata
     1861 acaccactgt cgccgtttca attctggtgt tgatcattgt ccgcgtcaca ccgttaagca
     1921 cctttttccc gtggattgaa aaacaggggc taagtatcgg gataatcatt ctgacgattg
     1981 gcgttatggc ccccatcgcc agcgggacgc taccgccctc aaccttgatt cactcgtttc
     2041 tgaactggaa atcgctggtg gcaattgcag taggcgttat tgtctcctgg ctgggtgggc
     2101 gcggcgtgac gttaatgggc agccagccgc aactggttgc tgggctgctg gtgggcactg
     2161 ttttaggcgt agcgctgttt cgcggcgtac cggttggccc gcttattgcc gcgggtctgg
     2221 tttcgctgat tgtggggaaa cagtagttaa tcccgcaata tatcggcctg gcgtttgccc
     2281 taggcctttc ttaaacatag taataaaggc cgttgttgaa tcatacccca gcgtatgagc
     2341 aactttctgt acagtatcgc ctttcactaa tccctgaagc gccataatca gctgtaactg
     2401 ctgacgccac tggcgaaaac tcaaccccgt ctctttcact atcagtcggg caagattgcg
     2461 ttcactcatc gcaaaaaagc cagcccattg ccccaatgac ccccactcga cgggcccttt
     2521 cgccatcatc tccaccatcg tgcggatttt aggatgagaa gaaacgggca ggtgcagctg
     2581 ttgttgtggc tgctgaggga gttcatcaaa caggacctga attaatctgc gggtcatcgg
     2641 ctcagcacgc tgagtcgtcg tcctgtttgc cagggttaaa attaattccc ggcataaggg
     2701 cgatattttc aatgtgcaac atgttgtcgg cattgtcact gcgctgggtt cgataaacag
     2761 aaaacagagt tcagcgttag ctgtcacttg attgctatgc ttcaccccgc ccggtatcca
     2821 aaccgcatat tgtggcggca ccatccacaa agcattttcc accgtacagg taattgcacc
     2881 atgtaatgct agtatcagct gtcctttgcg gtgctgatgt acaggactgg ttaactcatc
     2941 cgttccggcg tgaatacaaa atgcgaccgc ggcttcatgg tgtcggtcgg gttcatagcc
     3001 attgagattc aacctgtgca tcattttgtc cgaacttagc gataatttgt cattttagct
     3061 tgattcaaca taacaataaa aacggtaagg tacagcctcg tttgtaacaa tgagaagcat
     3121 atgacctgtt caacttcatt aagcggcaaa aacaggattg tccttatcgc tggcattctg
     3181 atgattgcca caacattacg cgtcaccttt accggcgcag cgccgttact ggatacgatt
     3241 cgttccgctt actcgctgac gacagcgcaa accggcttat taaccaccct gccattattg
     3301 gcctttgcgc taatctcacc tctggctgcc ccggtggcgc gacgttttgg tatggaacgt
     3361 agcctgtttg ccgcgttact tttgatctgt gcaggtatcg caattcgttc tctcccttcg
     3421 ccttacttat tatttggcgg tacagcggtc attggcggtg ggattgcatt aggcaatgtc
     3481 ttactgccag gattaattaa acgcgatttc cctcattccg tcgccagact taccggcgca
     3541 tattccctga caatgggagc tgcagcggca ctgggatcgg ctatggtcgt gccgctggct
     3601 ttgaacggtt ttggctggca aggcgcgttg ttcatgctga tgtgttttcc tctgctggct
     3661 ctttttttat ggctgccaca gtggcgaagt cagcaacatg caaatttgag tacctcacgc
     3721 gccttacata ctcggggtat ctggcgttcg ccacttgcct agcaggtaac attgtttctt
     3781 gggatcaact cactggtcta ttacgtgatt attggctggc ttccggcgat cctcatcagt
     3841 cacggctata gcgaagcaca ggcgggttca ctgcatggtt tgctgcaact agccacagca
     3901 gctcccggtt tgctgatccc acttttctta catcatgtga aagaccagcg tggtattgca
     3961 gcgttcgttg ctttgatgtg cgcagtgggc gcggttgggc tctgctttat gccagcgcac
     4021 gcgatcacct ggactctgct tttcggtttt ggttccggcg caacaatgat actggggttg
     4081 acgttcattg gtcttcgggc aagttctgcg catcaggcgg cggcactctc ggggatggca
     4141 caatccgtcg ggtatttgct ggcagcctgt gggccgccgc taatgggtaa aatacacgat
     4201 gctaacggta actggtccgt accactgatg ggtgttgcaa tactttcact actgatggcg
     4261 attttcggac tttgcgccgg gagagacaaa gaaattcgct aatatccggt gctatagtga
     4321 cgtgacaaat cttgcgtgaa agggagaaca aacacgatga atattcagtg caaacgcgtt
     4381 tatgatccgg ctgaacagag cgatggttat cgcgtactgg tcgaccgcct ctggccgcgc
     4441 ggtatcaaaa aaaccgattt agcccttgat gagtgggata aagaaatcac gccgtcaacg
     4501 gaactgcgca aagcctttca cggcgaagtc gtcgattttg caacctttcg cgagcaatat
     4561 cgcgcagaac tggcgcaaca cgagcaagaa ggaaagcggc tggcggacat cgccaaaaaa
     4621 cagccgctga ccctgctcta cgcagcaaaa aacaccacgc agaaccatgc gctggtgctg
     4681 gccgactggc tacgtagctt gtgattttag tacggcatcc ggcggttatt tttcaccagc
     4741 cggatggtcc cgtcgccaca atgcccattc atcaatcgtt tcaccgcccg gtaatttgca
     4801 attgttgctg accccttgcg ctgtctgcac tggaatgagc gtcccgccct tctgctggca
     4861 atagaccgac gccggatttg ccataccaat ctgcggcggt ttaggtgctt ctggctgaga
     4921 aggggttgaa caaccagcca ggaccagcaa gcaaggcaga acaaaactga taattttcat
     4981 ttattgatct cacatattta tccaagctta gagtatcgcg gtatcgtttt gttttgcagc
     5041 actattttta ttacattcac tcaaaacata ttacgtcttg tttcatcttt gttgatgatg
     5101 ttttatcatg cctgcaaaga ttaaataatc agcacttacc cgccgtatcc tggagttgtt
     5161 ccgtgtcaga tcagattatc gcccgcgtct cgcaatccct tgccaaagaa cagtcactgg
     5221 aaagtctggt ccgacagctt ctggagatgc tggaaatggt cactgatatg gaatcaacct
     5281 acctgaccaa agtggatgtc gaagcgcgcc tgcaacatat tatgtttgcc cgtaacagcc
     5341 agaaaatgca catcccggag aattttaccg tctcgtggga ttactcgtta tgcaaacgcg
     5401 ccattgatga aaactgcttt ttcagcgatg aagtccctga ccgttggggc gactgtattg
     5461 cggcacgcaa tcttggcatc accacatttc tgagcacgcc aattcactta ccggatggat
     5521 cattctatgg cacgctttgc gccgccagca gtgagaagcg ccagtggagt gaacgcgcgg
     5581 aacaggtttt acagttattc gccggactga ttgcacaata tattcaaaaa gaggcactgg
     5641 ttgaacagct gcacgaagcc aatgctgcgc tgattgcgca atcgtatacc gactcgttaa
     5701 ccgggctacc gaatcggcgg gcgatttttg aaaatctgac gacgctgttt tccctcgccc
     5761 ggcatcttaa ccataagata atgatcgcgt ttatcgatct ggataacttc aaattaatca
     5821 atgatcgttt tggtcataat agtggcgatc tgtttctcat tcaggttggc gagcgcctta
     5881 atacgctcca gcaaaatggc gaagttattg gtcgtctcgg tggtgatgag tttttggttg
     5941 tttcactaaa caacgagaat gcggatattt cgtcgctgcg agaacgtatt caacagcaaa
     6001 tacgtggaga atatcactta ggtgatgttg atttgtatta tcccggtgcc agtcttggca
     6061 tagtagaagt cgatcctgaa acaaccgatg cagacagtgc cctgcatgct gccgatattg
     6121 cgatgtatca ggagaaaaaa cacaaacaga aaacaccttt tgtcgcgcat ccagcgctac
     6181 attcctgagg cgtattcaca tccttttgat tggtgataac atgcgaatcg gtattatttt
     6241 tccggttgta atcttcatta cagcggtcgt atttttagca tggtttttta ttggcggcta
     6301 tgctgccccg ggagcataaa gatgaaaaaa acaacgatta ttatgatggg tgtggcgatt
     6361 attgtcgtac tcggcactga gctgggatgg tggtaacgtc acctctaaaa aatagcaaag
     6421 gctgcctgtg tgcagccttt gtgcaattta agcgttaact tttaatcttc ctgtagataa
     6481 atagcacgac aatcgcacca ataacggctg aagcatttcg tctgcagtct gaaccgcagg
     6541 caaccatttt tcagcaagtc tgcgctggga atcgctggct ttatcccatt tgctccacag
     6601 tgcctcacga tcgtaatcgt gcgcttccag cgtggggcgg gcgatttcaa aatacccctg
     6661 gcttgtttct atttgctcag attttaataa caactcaaca cgtgctgccg ctggtagaac
     6721 tgatatatga aattccaaag cctttgaacc cgtacgcttg cgacgtagtt ctggatgatt
     6781 ggctgcaagc ttttcaagat tgcggcgaat actcacctca tgtttaggaa agcatgacat
     6841 tcctacgcat tcttgcgctg ttacccaatg ggttaacatg atgttttctc catataccga
     6901 gaaggccaaa tctgggctgg ttccatatcc aaatatctag caatgattgc ttcgtatcgt
     6961 ggtatatggc agcgaaatac gttacgcata gtgtcaggct ttaagcctgc ctctctggat
     7021 aattgagata gtgtcttccc cttcatacga acagcagcct ttatctgctc tgcgtgccag
     7081 tcttggtttt ttgctgacat tctgcgatcc ttactctctt aacgttttcg gtaactgata
     7141 ccggctaccg ctatcgggaa aaacgatatc gcagaaaaat gcgcatttca agagctatgc
     7201 acatttttat tttaaccaat gcaaatcaaa gaattctgta aaattcttta tataacagag
     7261 agattgagca tgacaggtga aaaagaaagc aaaaatgcag agcgcatttt tatgccccgg
     7321 aaataatgcg tttcaaggaa cgccttgaag aggcaatgaa tggggaa
//
LOCUS       DAJVQH010000162         7294 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJVQH010000162 DAJVQH010000000
VERSION     DAJVQH010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.162"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            94..1205
                     /gene="yneK"
                     /locus_tag="OQM87_003728"
                     /pseudo
     CDS             94..1205
                     /gene="yneK"
                     /locus_tag="OQM87_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            1355..2545
                     /gene="ydeA"
                     /locus_tag="OQM87_003729"
     CDS             1355..2545
                     /gene="ydeA"
                     /locus_tag="OQM87_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCS1509699.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2570..3235)
                     /gene="marC"
                     /locus_tag="OQM87_003730"
     CDS             complement(2570..3235)
                     /gene="marC"
                     /locus_tag="OQM87_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCS1509700.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3447..3881
                     /gene="marR"
                     /locus_tag="OQM87_003731"
     CDS             3447..3881
                     /gene="marR"
                     /locus_tag="OQM87_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCS1509701.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3901..4306
                     /gene="marA"
                     /locus_tag="OQM87_003732"
                     /pseudo
     CDS             3901..4306
                     /gene="marA"
                     /locus_tag="OQM87_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
     gene            4338..4556
                     /gene="marB"
                     /locus_tag="OQM87_003733"
     CDS             4338..4556
                     /gene="marB"
                     /locus_tag="OQM87_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCS1509702.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4587..5486)
                     /gene="eamA"
                     /locus_tag="OQM87_003734"
     CDS             complement(4587..5486)
                     /gene="eamA"
                     /locus_tag="OQM87_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCS1509703.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5750..6868
                     /gene="ydeE"
                     /locus_tag="OQM87_003735"
     CDS             5750..6868
                     /gene="ydeE"
                     /locus_tag="OQM87_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCS1509704.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            6995..7090
                     /gene="mgtS"
                     /locus_tag="OQM87_003736"
     CDS             6995..7090
                     /gene="mgtS"
                     /locus_tag="OQM87_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCS1509705.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         1905 a         1775 c         1659 g         1955 t
ORIGIN      
        1 acagcgccac gcatcacctg cctttgatag ttcttgcagc tcaaatgccc tatatagtga
       61 tgttccgatg acttatgact atatggggca aatatggtta cgccagtaag catcagcaat
      121 tacatatctc ttcctgatga ttttcccgct cgtaatattg cacctcaagt aaaagaagtt
      181 ttaaaggatt ttattgatgc actcagtacc ataatatgtg atgaagagtg gcgcacgtct
      241 ttaaacacca actcagccac aaaaaagata tttaacaacc ttgataactt atcatatatt
      301 cagaggactt cttttcgggg taacgacaca ctatacaatg aaaaggttca gtttaaactc
      361 acttatcccg tcaagaatgg aagacacaaa gaaaatattg aatttcaggt agtaataaat
      421 ttaagcccta tttagataat tttcgccatg atggagaaat taatattttt tgcgctccca
      481 acccaaagcc tgtcactatg gggcgcgtat ttcagaccgg cgtcgagcgt gtgctctttc
      541 tgtttatgaa tgattttatt gaacaatttc caatgatcaa ccttggcgcc cccatcaaaa
      601 gagcgcatac accacatatt gaacccctgc ctccggatca ccataccgct gcagattact
      661 tacgccagtt tgatttgctt gtcctgaatt ttatctctcg cggtaatttt gtcatactcc
      721 cccgattatg gaataactct gaggttcaca gatggttcgt caataaagat cctaacttga
      781 tcaccgccat tctcgacata acggacagcg aattaaaaga ggatttattg caaagcctga
      841 tggattcatt aggttctaac aaacatgtac aacccgaagt ctgcatctgc ttcttatccc
      901 ttttagcaga acaagaatct cctcattttc aggacttgtt tttatttttc gccaatatgt
      961 tattgcacta tcaccaattt atgaatccca atgaaagtga tctgaatgac gtgttgatgc
     1021 cagcatcatt aagtgatgat aaaattatca aacatatggc gcgcaggacc ctcaaactat
     1081 ttgtaaaaaa tgaaacgccg ccaaaagtta ctcacgaaga cctggtgaaa aacaggcctc
     1141 gctcccctgt cagaccacct atacccgcaa ccgccaaaac gccagacctc cctgaacgtc
     1201 attaaaccgt gatgttaccg actctctgac gcggaacaga atcagcgtca gagaaacgga
     1261 aaacgcgatc cagatcacaa atgcattgta ttcacatcat taaccgtttt aagatcattt
     1321 catcactttt tcgcaactca cccgataatc tgttatgaca acaaacactg tttcccgcaa
     1381 agtggcgtgg ctacgggtcg ttacgctggc agtcgccgcc ttcatcttca acaccaccga
     1441 atttgtccct gttggcctgc tctctgacat tgcgcaaagt tttcacatgc aaaccgctca
     1501 ggtcggcatc atgttgacca tttacgcatg ggtagtagcg ctaatgtcat tgccttttat
     1561 gttaatgacc agtcaggttg aacggcgcaa attactgatc tgcctgtttg tggtgtttat
     1621 tgccagccac gtactgtcgt ttttatcgtg gagctttacc gttctggtga tcagtcgcat
     1681 tggtgtggct tttgcacatg cgattttctg gtcgattacg gcgtctctgg cgatccgtat
     1741 ggctccggcc gggaagcggg cacaggcctt gagtttaatt gccaccggta cagcactggc
     1801 gatggtctta ggtttaccgc tcgggcgcat tgtggggcag tatttcggct ggcgaatgac
     1861 cttcttcgcg attggtattg gggcgcttgt tacccttttg tgcctgatta agttacttcc
     1921 cttactgcct agtgagcatt ccggttcatt gaaaagcctc ccgctattgt tccgccgccc
     1981 ggcattgatg agcatttatt tgttaactgt ggtggttgtc accgcccatt acacggcata
     2041 cagctatatc gagccttttg tgcaaaacat tgcgggattc agcgccaact ttgccacggc
     2101 attactgtta ttactcggtg gtgcgggcat tattggcagc gtgattttcg gtaaactggg
     2161 taatcaatat gcgtctgcgt tggtaagtac ggcgattgcg ctgttgctgg tgtgtctggc
     2221 actgctgcta cctgcggcga acagtgaaat acacctcggg gtgctgagta ttttctgggg
     2281 gatcgcgatg atgatcatcg ggcttggtat gcaggttaaa gtgctggcgc tggcaccaga
     2341 tgctaccgac gtcgcgatgg cgctattctc cggcatattt aatattggaa tcggggcggg
     2401 tgcgttggta ggtaatcagg tgagtttgca ctggtcaatg tcgatgattg gttatgtggg
     2461 cgcggtgcct gcttttgccg cgttaatatg gtcaatcatt atatttcgcc gctggccagt
     2521 gacactcgaa gaacagacgc aatagttgaa aggcccattc gggccttttt taatggtacg
     2581 ttttaatgat ttccaggatg ccgttaataa taaactgcac acccatacat accagcagga
     2641 accccatcag gcgggagatc gcttcaatgc cacccttgcc caccagccgc ataattgcgc
     2701 cggagctgcg taggcttccc cacaaaataa ccgccaccag gaaaaagatc agcggcggcg
     2761 caaccatcag tacccaatca gcgaaggttg aactctgacg cactgtggac gccgagctaa
     2821 taatcatcgc tatggttccc ggaccggcgg tacttggcat tgccagtggt acaaaggcaa
     2881 tattggcact gggttcatct tccagctctt ccgacttgct tttcgcctcc ggtgaatcaa
     2941 tcgctttctg ttgcggaaaa agcatccgaa aaccgataaa cgcgacgatt aagccgcctg
     3001 caattcgcag accgggaatc gaaatgccaa atgtatccat caccagttgc ccagcgtaat
     3061 acgccaccat catgatggca aatacgtaca ccgaggccat caacgactga cgattacgtt
     3121 cggcactgtt catgttgcct gccaggccaa gaaatagcgc aacagttgtt aatgggttag
     3181 ctaacggcag caacaccacc agccccaggc caattgcttt aaacaaatct aacattggtg
     3241 gttgttatcc tgtgtatctg ggttatcagc gaaaagtata aggggtaaac aaggataaag
     3301 tgtcactctt tagctaacct tgcatcgcat tgaccaaaac ttgaaccgat ttagcaaaac
     3361 gtggcatcgg tcaattcatt catttgactt atacttgcct aggcaatatt atcccctgca
     3421 actaattact tgccagggca actaatgtga aaagtaccag cgatctgttc aatgaaatta
     3481 ttccattggg tcgcttaatc catatggtta atcagaagaa agatcgtctg cttaacgagt
     3541 atctgtctcc gctggatatt accgcggcac agtttaaggt gctctgctct atccgctgcg
     3601 cggcgtgtat tactccggtt gaactgaaaa aagtgttgtc ggtcgacctg ggagcactga
     3661 cccgtatgct ggatcgcctg gtctgtaaag gctgggttga aaggttgccg aacccgaatg
     3721 ataagcgcgg cgtactggta aaacttacca ccagcggcgc ggcaatatgc gaacaatgcc
     3781 atcaattagt tggccaggac ctgcatcaag aattaacaaa aaacctgacg gcggacgaag
     3841 ttgcaacact tgagcatttg cttaagaaag tcctgccgta aacaaaaaag aggtatgacg
     3901 atgtccagac gcaatactga cgctattacc attcatagca ttttggactg gatcgaggac
     3961 aacctggaat cgccactgtc actggagaaa gtgtcagagc gttcgggtta ctccaaatga
     4021 gcgttcgggt tactccaaat ggcacctgca acggatgttt aaaaaagaaa ccggtcattc
     4081 attaggccaa tacatccgca gccgtaagat gacggaaatc gcgcaaaagc tgaaggaaag
     4141 taacgagccg atactctatc tggcagaacg atatggcttc gagtcgcaac aaactctgac
     4201 ccgaaccttc aaaaattact ttgatgttcc gccgcataaa taccggatga ccaatatgca
     4261 gggcgaatcg cgctttttac atccattaaa tcattacaac agctagttga taacgtgaca
     4321 acgtcactga ggcaatcatg aaaccacttt catccgcaat agcagctgcg cttattctct
     4381 tttccgcgca gggcgttgcg gaacaaacca cgcagccagt tgttacttct tgtgccaatg
     4441 tcgtggttgt tcccccatcg caggaacaac caccgtttga tttaaatcac atgggtactg
     4501 gcagtgataa gtcggatgcg ctcggcgtgc cctattataa tcaacacgct atgtagtttg
     4561 ttctggcccc gacatctcgg ggcttattaa cttcccacct ttaccgcttt acgccaccgc
     4621 aagccaaata cattgatata cagcccggtc ataatgagca ccgcacctaa aaattgcaga
     4681 cccgttaagc gttcatccaa caatagtgcc gcacttgcca gtccgactac gggcaccagt
     4741 aacgataacg gtgcaacccg ccaggtttca tagcgtccca gtaacgtccc ccagatccca
     4801 taaccaacaa ttgtcgccac aaacgccaga tacatcagcg acaagatggt ggtcatatca
     4861 atagtaacca gactgtgaat catggatgcg gaaccatcga gaatcagcga ggcaacaaag
     4921 aagggaatga ttgggattaa agcgctccag attaccagcg acatcaccgc cggacgcgtt
     4981 gagtgcgaca tgatcttttt attgaagatg ttgccacacg cccaactaaa tgctgcagcc
     5041 agggtcaaca taaagccgag catcgccaca tgctgaccgt tcagactatc ttcgattaac
     5101 accagtacgc caaaaatcgc taaggcgatc cccgccaatt gtttgccatg cagtcgctcc
     5161 ccgaaagtaa acgcgccaag cacgatagta aaaaacgcct gtgcctgtaa caccagcgaa
     5221 gccagtccag caggcatacc aaagttaatg gcgcagaaca gaaaggcaaa ctgcgcaaaa
     5281 ctgatggtta atccataccc caagagcaaa ttcagtggta ctttcggtcg tgcaacaaaa
     5341 aagatggccg gaaaagcgac cagcataaag cgcaaaccgg ccagcatcag cggtggcatg
     5401 ttatgaagcc ccactttgat gaccacaaaa tttagccccc atacgaccac taccagtagc
     5461 gccaacaccc catcttttcg cgacattcta ccgcctctga atttcatctt ttgtaagcaa
     5521 tcaacttagc tgaatttact tttctttaac agttgattcg ttagtcgccg gttacgacgg
     5581 cattaatgcg caaataagtc gctatacttc ggatttttgc catgcgtccg atggttaata
     5641 acaacgacga ggcaagaagg gcgctggtag agcgtcgtag ggataagttc atttgtctgt
     5701 ccggcagtgc gtttcgttat gttttatttt agagatgtaa agaaatagca tggctacgct
     5761 gccatttatg accatttact tgagtcgcca gtacagcctg agtgtcgatc taatcggtta
     5821 tgcgatgaca attgcgctca ctattggcgt cgtttttagc ctcggttttg gtatcctggc
     5881 ggataagttc gacaagaaac gctatatgtt actggcaatt accgccttcg ccagcggttt
     5941 tattgccatt cctttagtga ataacgtgac gctggttgtg ctcttttttg ccctcattaa
     6001 ctgcgcctat tctgtttttg ctaccgtgct gaaagcctgg tttgccgaca atctttcgtc
     6061 caccagcaaa acgaaaatct tctcaatcaa ctacaccatg ctaaacattg gctggaccat
     6121 cggtccgccg ctcggcacgc tgttggtgat gcagagcatc aatctgccct tttggctggc
     6181 agctatctgt tccgcgtttc ccatgctttt cattcaaata tgggtaaagc gcagcgagaa
     6241 aatcatcgcc acggaaacag gcagtgtctg gtcgccgaaa gttttattac aagataaagc
     6301 actgttgtgg tttacctgct ctggttttct ggcttctttt gtaagcggcg catttgcttc
     6361 atgcatttca caatatgtga tggtgattgc tgatggggat tttgccgaaa aggtggtcgc
     6421 ggttgttctt ccggtgaatg ctgccatggt ggttacgttg caatattccg tgggccgccg
     6481 acttaaccca gctaacatcc gcgcgctgat gacagcaggc accctctgtt tcgtcatcgg
     6541 actggtcggt tttatttttt ccggcaacaa cctgctattg tggggtatgt cagctgcggt
     6601 atttactgtc ggtgaaatca tttatgcgcc gggcgagtat atgttgattg accatattgc
     6661 gccgccagga atgaaagcca gctatttttc cgcccagtct ttaggctggc ttggtgccgc
     6721 gattaaccca ttagtgagtg gcgtagtgct aaccagcctg ccgccttcct cgctgtttgt
     6781 catcttagcg ttggtgatca ttgctgcgtg ggtgctgatg ttaaaaggga ttcgtgcaag
     6841 accgtggggg cagcccgcgc tttgttgatt taagtcgaac acaataaaaa tttaattcag
     6901 ccttcgttta ggttatctct gctaatatct ttctcattga gatgaaaatt aaggtaagcg
     6961 aggaaacaca ccacaccata aacggaggca aataatgctg ggtaatatga atgtttttat
     7021 ggccgtactg ggaataattt tattttctgg ttttctggcc gcgtatttca gccacaaatg
     7081 ggatgactaa tgaacggaga taatccctca cctaaccggc cccttgttac agttgtgtac
     7141 aaggggcctg atttttatga cggcgaaaaa aaaccgccag taaaccggcg gtgaatgctt
     7201 gcatggatag atttgtgttt tgcttttacg ctaacaggca ttttcctgca ctgataacgg
     7261 atcgttgaca cagtagcatc agttttctca atga
//
LOCUS       DAJVQH010000163         7246 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJVQH010000163 DAJVQH010000000
VERSION     DAJVQH010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.163"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..473
                     /locus_tag="OQM87_003737"
     CDS             <1..473
                     /locus_tag="OQM87_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCS1509706.1"
                     /translation="RNPKKLADVVASLNADSEYAAIFVPGGHGALIGLPESQDVAAAL
                     QWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTPDIGYMPGHLTW
                     YFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQEMLAAYAS"
     gene            complement(581..1939)
                     /gene="hprS"
                     /locus_tag="OQM87_003738"
     CDS             complement(581..1939)
                     /gene="hprS"
                     /locus_tag="OQM87_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCS1509707.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(1939..2610)
                     /gene="hprR"
                     /locus_tag="OQM87_003739"
     CDS             complement(1939..2610)
                     /gene="hprR"
                     /locus_tag="OQM87_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCS1509708.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2743..3156
                     /gene="hiuH"
                     /locus_tag="OQM87_003740"
     CDS             2743..3156
                     /gene="hiuH"
                     /locus_tag="OQM87_003740"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCS1509709.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3265..4269
                     /gene="msrP"
                     /locus_tag="OQM87_003741"
                     /pseudo
     CDS             3265..4269
                     /gene="msrP"
                     /locus_tag="OQM87_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4270..4904
                     /gene="msrQ"
                     /locus_tag="OQM87_003742"
                     /pseudo
     CDS             4270..4904
                     /gene="msrQ"
                     /locus_tag="OQM87_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            5162..5812
                     /gene="zinT"
                     /locus_tag="OQM87_003743"
     CDS             5162..5812
                     /gene="zinT"
                     /locus_tag="OQM87_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCS1509710.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMISIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(6890..7159)
                     /locus_tag="OQM87_003744"
     CDS             complement(6890..7159)
                     /locus_tag="OQM87_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1509711.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
BASE COUNT         2035 a         1652 c         1511 g         2048 t
ORIGIN      
        1 tccgtaatcc gaagaaactc gcggatgttg tcgccagcct caacgctgat agcgaatatg
       61 cagcaatctt tgttcctggt gggcatggcg cacttattgg tttaccagaa agccaggacg
      121 tggctgccgc tttacagtgg gcaatcaaaa atgaccgttt tgttatctcc ctttgccacg
      181 gtccggcggc ttttctggca cttcgccaca gcgataaccc actgaatggt tattctattt
      241 gcgcattccc agacgccgca gacaaacaaa cgccagatat tggctatatg ccgggtcatc
      301 tcacctggta cttcggcgaa gagttgaaga aaatgggcat gaacatcatt aatgacgaca
      361 tcaccgggcg agtacataag gaccgtaaac gtctcaccgg cgacagtcct tttgcagcga
      421 atgcgttggg taaactggcg gcgcaggaaa tgctggcagc ttacgcgagt taatccctgt
      481 tacattgtga tgtaagcgca taatcaatcg cagccacgtt aatactcacg acgtggctgc
      541 gttttttcac ggttaactta tggcgtactg aagcccgatg ttaatttctt tgcggtaacg
      601 taattcggaa cacgttatgc ttactgagat agtgatacgt agcacttcct ccatgtaatt
      661 cggcaatcgc tttgactaaa gaaaggccaa gtccctgacc tacggaatga cgcgaattat
      721 ctccccgcca aaatctacgg aagagttttt caggctcatt aattttcgtt ccaggactgg
      781 cgatatcaat attaagagag ccgttggcat caagaaaact ggttatatga atacgcgatt
      841 tttctggtga atatcgaatg gcgttaacaa taagattcga taacattcgt tgcagtaaaa
      901 ttttatccgc aaagatttgt tgattgcact tgaccttaaa gcaaatctct ttctcgtctg
      961 aaagagattc aagatagtcc aaaagatttt caacttcctt attgagagaa agcgaatcca
     1021 gtttcaccaa aacattgttt ttgtctgccc tggcaagaaa cagtatattc tctgttaacc
     1081 gcgaaatatt ttccagctct tcaatgtttc cggcaattgt tttttgatat tcagcgatac
     1141 ttctgatttg actgagcgta acctgattct gacccagtaa tgcattaatt ggcgttctaa
     1201 gttcatgagc gagatcgtca gcaaattgac taaggcgctc aaaatctttg actaaagcat
     1261 gatgcatttt attcaacgcc tgcccaagag gttttagttc tctcggtaat gcgttaacct
     1321 caaccggcac tcggctatcg ttataattca gcgcttccgt tacgccactc aactttttga
     1381 tctctcgcaa ccccgtcctg attaacaacg gacttaatac tgagcacagt ataatggcga
     1441 caatgcaaat tataatgcta ttaattttat actgttcaag catattatgt ctggctgaag
     1501 ccaatttagc cacagtaacc gttaatgatg gcgaaacttc atcaatgtta attcgtaaag
     1561 catctatctc tgtatcatta ataatgcttc tgtaaatgcc tgctgcgctg attgtctcac
     1621 tagcaggtat gttatttaac atgtcatcac tgacatttgt ccggttaaca attttattga
     1681 tgccatcact atgaatgatc aaaatatcct gactaacatc catcatccgg ttaaagtaca
     1741 caggtaacgt atctggattt accccatcaa ttaacaactg cttgatctgc gctgtccggt
     1801 taatgagtgt tgtatcatcg cgccatttca actcacttgc caggccatta tagagagtcc
     1861 atacaattcc agcgccagca acagactgca gaaatataaa aagcaaggtt aaacggacgg
     1921 ttatagataa tcttttcatt atttttttac cgctacgaat gaatagccca tcccccggat
     1981 tgtggcgatt agcttttcag gaaaaggatc atcaactttt gcgcggagcc tgcgaatggc
     2041 gacatccacc gtattggtat cactatcaaa gttgattccc caaatttcac tcgcaataac
     2101 cgttctgggt ataatctcgc cagctctgga ggccagtagc caaagtaact gaaactcctt
     2161 gcgcgtcagt gtaatactga tattgtccct gctgacactt tgactgacag agtccatttt
     2221 taagccactg atttctaatg ttgaattcag agcgtgatgt tgccttaatt gtgcccgaac
     2281 ccttgctaac aactcagaaa atgaaaaagg ttttaccaga taatcatttg ccccactgtc
     2341 caaccctctg actctgtcat cgacagaatc ccttgcagta aggcaaataa caggggtttg
     2401 ctttgccgtt cttaacgttt gtaagatctg ccagccatcc atacccggaa gcataatatc
     2461 cagaatgatc aatgcataat catccttcag cgcaagataa agcccatctc tgccatcaga
     2521 aacggcatca atgacataac ccgcttcgga aagcccctgc gttacccatt cctgggtcct
     2581 ttgattatct tcaataagta gaatcttcat gaaaataata tgccataaat catatgttat
     2641 agaaacaacc tggttcatta caaaattgta atgctgctgt aaggttaccc tggacgtttt
     2701 ttcgctatcc tcaaaactca ttcacatgac aaggatataa acatgttaaa gcgttattta
     2761 gtactctcgg tagcaacggc agcattttca ttaccttctt tggtttatgc cgcacaacaa
     2821 aacattctta gcgtgcacat tttgaaccag caaacaggaa aacccgctgc cgacgtgaca
     2881 gtcactcttg aaaagaaagc ggataacggc tggttacaac ttaataccgc caaaacagat
     2941 aaggatggac gaattaaggc actgtggccc gagcaaactg cgactactgg cgattaccgt
     3001 gtcgtattta aaaccgggga ctatttcaag aaacaaaatc ttgaaagttt cttccctgag
     3061 attcccgttg aatttcatat taataaagtg aacgagcatt atcatgtgcc tttgttactc
     3121 agccaatatg ggtattcaac ctatcgtggc agttaatgcg taaatatgta aaagctgatc
     3181 tctgctactg ttagggaggt gactccctga cagattaaca gtaaacagct cttgttggtt
     3241 aacgacaaaa aagtgtgatg gcttatgaaa aagaatcaat ttttaaaaga atcagatgtt
     3301 acggccgagt cggtattctt tatgaagcgt cggcaggtgt taaaagcact gggcatcagc
     3361 gcagctgcat tttctttgcc tcacgctgcg catgccgatc tgcttagctg gtttaaaggg
     3421 aacgatcgtc cgcccgcccc cgccggaaaa ccgctggagt tcagcaagcc tgccgcctag
     3481 caaaataacc tgccactgac gccagcagat aaagtctccg gttataacaa cttctatgaa
     3541 ttcgggctgg ataaagacga tcccgcggct aatgctggta gcctgaaaac cgatccatgg
     3601 acactgaaaa tcagcggcga agtggcaaaa ccattgaccc tcgatcatga tgatttaacc
     3661 cgtcgcttcc cgctggaaga gcgtatttat cgtatgcgct gcgtggaagc gtggtcgatg
     3721 gtggtgccgt ggattggttt tccgctacac aaattgctgg cgcttgccga acccaccagc
     3781 aatgcgaagt atgtcgcttt cgaaacgatt tatgcgccgg aacagatgcc tggccagcag
     3841 gaccgcttta tcggcggcgg gctgaaatat ccttatgtcg aaggattgcg tctcgacgaa
     3901 gcaatgcatc cgctcacact gatgaccgta ggtgtttatg gcaaggcgtt accaccgcaa
     3961 aatggcgcgc cggtgcgact gattgtgccg tggaaatatg gctttaaagg gattaaatcg
     4021 atcgtcagta ttaagctgac ccgcgagcgt ccgccaacca cctggaatct ggcagcgcct
     4081 gacgaatacg gtttttacgc caacgttaat ccgcatgttg atcacccgcg ctggtcacag
     4141 gctaccgaac gatttattgg ttcaggcggc attctcgatg ttcagcgcca gccaacacta
     4201 ctgtttaatg gttacgccga ccaggtggca tcgctgtatc gtggcctgga tttgcgggag
     4261 aatttctaaa tgcgtctgac agcaaaacag gtgatatggc tgaaagtttg cctgcatctt
     4321 gccggattgt tgccgtttct ctggctggtc tgggcgatca atcacggtgg actgggtgcc
     4381 gatccggtga aagatattca gcattttacc ggtcgcactg cactgaaatt tttgcttgcg
     4441 gccttgttaa tcacacctct ggcacgctac gcaaaacagc cgttattgat acgcactcgc
     4501 cgcctgttag gcttatggtg ctttgcctgg gcgacactgc atttaaccag ttacgcattg
     4561 ctggagttgg gcgtgaataa tctggcgtta ttaggtaagg agttaattac ccggccttat
     4621 ttaacgttag gaattatcag ctgggttatt ttgcttgctt tagcgttcac ttcaacccag
     4681 tcgatgcagc gaaaactcgg caaacactgg caacagttgc ataacttcgt ctatcttgtc
     4741 gcgatcctgg ccccaataca ttatctgtgg tcagtgaaga ttatttcacc gcagccactc
     4801 atctacgctg ggctggctgt actgctttag ccttacggta taagaagttg ctttcccttt
     4861 tcaaccggtt acgcaagcag gcccacaaca aattatctct ataacttcat attgccgaca
     4921 aacttcgcgt tatcccggcc ggatgcggcg cgagcgccct atccggccca cacagatcgc
     4981 atcaagttat gtttcgtttg tcatcaacct ttcatcacca gaataattta ttttatttca
     5041 ataagatagt tcaatacacc tgagaatacc atgctctcat ttcctaatga gttgttgcat
     5101 tttttcatat gttacaatat aacattacac ataatgtaca ttaactctgg aggaaattgt
     5161 tttggcgatt cgtcttcata aactggctgt tgctttaggt gtctttattg ttagcgctcc
     5221 tgccttttcg catggtcatc attctcatgg caaaccctta acagaggtcg aacaaaaagc
     5281 tgctaatggt gtttttgatg ataccaatgt acaaaaccga acgctcagtg actgggatgg
     5341 agtctggcaa tccgtttacc ctttactgca aagtggcaaa cttgatcccg tctttcagaa
     5401 gaaagcggat gcagataaaa ccaaaacatt tgctgaaatt aaagattatt atcgcaaagg
     5461 ttatccgaca gatatcgaaa tgattagcat tgaggacggc attgtcgaat tccatagaaa
     5521 taatgaaaca acatcctgta aatatgatta cgatggatac aaaatactca cctataaatc
     5581 aggcaagaaa ggcgttcgtt acttattcga atgtaaagat cctgaaagca aagcccctaa
     5641 atatatacaa tttagcgatc atataattgc accacgaaaa tcatctcatt ttcacatttt
     5701 tatgggtaat gattcccagc aatctttatt gaatgaaatg gaaaactggc caacgtatta
     5761 tccctatcag ttgagtagcg aagaagtggt cgaggagatg atgtctcatt gatttttttg
     5821 cgctgacttc agttatttaa cagaacagtg cctggtagta acaacaataa tttaccaaga
     5881 caaataagta agacaaactc agacacgctc atacttgagt cgtgtctttt ttattatgtc
     5941 tgtagaaaaa aactgagaaa aaggcgaaca ataaatacac aactcagcca tacagactgg
     6001 cagcgcaaat gtttccagga gcaagtcatc tgtcatggta aagctaacca gaaatatagg
     6061 ttaattttgt tgtttaaacc ttttttactt gttagtataa tacaaaggct ttcaaaaaag
     6121 ctgggaaatt caacaactct tggaagttat ggactttgtc ccgcggcttt aatctgacgg
     6181 ccgcgttcct tttttggctc aattattgac cccttctcta cgcaacttca gttcccgcca
     6241 gcaactttgc ggcagtttca taggatcaat gtctaaaaga ataatggtga ccgataagaa
     6301 aacgactgaa taactgtaga ttttcgctcg aaaccttcct gtcagctcca tagcgaatca
     6361 agcgctgaat gtcacagtat cgaacagaaa acagtgacga tctaaccctt caagaatatt
     6421 ctacgattgt tctgtttagg aaaagcaagg cgggaagtcg ggagataagt cattgataaa
     6481 gtggcggaga gagggggatt tgaccccccg gtagagttgc ccctactccg gttttcgaga
     6541 ccgatgatac agagtcgaaa aatcaaagag ttaaaaattt ttcttggaaa aaatgtctta
     6601 aaatcgtaca cgaaaaatca atgaattaca taacattaaa gaagaatatt ccaagccttc
     6661 ttgagtccac cacctgtttt caggtgtcat tcagacgtga tttgccccag attgggaaag
     6721 cacgcttttt tctcagctta ttccgttttg cctgtgtgtt gaataattcc actgcagtta
     6781 tataaaatcg gtaacggctg gaaatcattc aatactcgca ctatcgaaag tttaccagcc
     6841 aaccgcagca cgtcctgcat acgtcgtgtc tgcggttttt ctttttcgct tacattgtgt
     6901 ctggttcttc cggccactca atatcaggtg cagttgatgt atcaacacgg ttcagcaaca
     6961 cccgatactt cttccaggct tccagcaacg agttttcttc ctccgttgcg atctccagat
     7021 ctacagcatc ctgaagtggc gcaatatgct cactgaatcc ctggatgtag aactgtgtgg
     7081 tgacggtctt ccagccattc ggctcctgct gtatcgaagc ataccaggct atttcaatat
     7141 cgctatgctg cggcagcatt taaccccttg taattcatcg ccataattga tttaattcac
     7201 aaataaaact ataatatggt gaaattaatg aaaaaaaaca cagatg
//
LOCUS       DAJVQH010000164         6886 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJVQH010000164 DAJVQH010000000
VERSION     DAJVQH010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.164"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(141..3053)
                     /locus_tag="OQM87_003745"
     CDS             complement(141..3053)
                     /locus_tag="OQM87_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509712.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3056..3172
                     /locus_tag="OQM87_003746"
     CDS             3056..3172
                     /locus_tag="OQM87_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1509713.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3536..4378
                     /locus_tag="OQM87_003747"
     CDS             3536..4378
                     /locus_tag="OQM87_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1509714.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4381..5469
                     /gene="capU"
                     /locus_tag="OQM87_003748"
     CDS             4381..5469
                     /gene="capU"
                     /locus_tag="OQM87_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCS1509715.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNEPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5474..6424
                     /gene="virK"
                     /locus_tag="OQM87_003749"
     CDS             5474..6424
                     /gene="virK"
                     /locus_tag="OQM87_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCS1509716.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6489..>6886
                     /locus_tag="OQM87_003750"
     CDS             6489..>6886
                     /locus_tag="OQM87_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1509717.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVI"
BASE COUNT         1858 a         1536 c         1615 g         1877 t
ORIGIN      
        1 taccgcgcac atggcctgcc cgtgtcagaa aatctgctgg agcaggattt ttacgccagt
       61 ggtccgaacc agaagtgggc gggagacatc acgtacttac gtacagatga agttcggtta
      121 catcctgtca gcacggaacc tcacgctttt tgagcggcac gacggatccc ccccacccag
      181 tatccgacag acttttcact gcgttcgtaa aaacccttct tccctcccag catatccact
      241 aattccggtg acatatcctg cagataagtc tgcaaatcat gccccagatc ttccggtgac
      301 tgcaactcat aaaccgcgcg ccacacatca ccctgcctta ttttaagccc cgggttaaga
      361 atatactgtc cgtggttcag cctcagaaaa agcttttttc cataaggatt tttgctgtta
      421 acttcatgtt gagagagctg gctgctgata taactccttc gtttgcggcg ttccggcagc
      481 aatgcttcag gaagatgaac cacacactct gccagtcgtg ccgcactgaa ggcttccgaa
      541 aaaaacagag cacttcccag atgcgtgtgg aacagcgcct gcatcaggat caccagaaac
      601 attgccatct gccggttgtc cagtttgatg aggcgtcctt ccacctgcag tgacagactt
      661 tcaggtttca gcagggtata caactgcgca cttttctgtt gcgcataacg gggcgtactg
      721 accgccgctg acagaacttg atgataagcc gccagcccgt cattaccggt cagcaaggga
      781 tctgcaccac gctccagcag taactgaacc agtgcaatat tacctgcccg ggctgctgac
      841 atcagcggag taagattaaa cggcgaacgg tgatcaacac cataacgttc aatatcccgc
      901 aacacggcaa ccgggttatt cattgagtat ggccggaaat acttcagctc cagtaactgc
      961 agagtcttcg ttctgggctg ccttgtgggc ttaaaatctt cccggtacag tgcattatac
     1021 agcgggaaca gggaataaat cagactgtac tccagcgcct gcagttgtat ggttttatcg
     1081 gctgtacctg cgaggatatg ctgtttccac tgtcgtgcat cctctgcggt gataatctgc
     1141 catggcatct cagatgtctg caggatccgg ctacggatgg tgtccgcctg ctcctgcttc
     1201 ccctgttttt caagcagatg tgcggttttc tgccactcat cacggctgga aatctcttcc
     1261 tgcagattca gaggctcttc ctgatgcgtg agcgcaagca gactccacag aggatgctcc
     1321 acctgctgtt caacaagata cacattgtgt gtagcacggg tgagcgcaac atacagcgcg
     1381 ttggtaaaaa atttatagat ttccgctgaa cgatcctgcc tgtcccgcgg acgggaataa
     1441 cgggcctcgc cctcaagatc agccggtgta agaccctcgc aaatatcatc aaatgcctgc
     1501 cgggcagcgc ttactatatt gtaaaggatc accgtttcgt attccagccc cttaacctcc
     1561 tgaaccgaaa agaccagcgg cgtactgaac cagcatcgcg cctgtgcttt ctgttccgga
     1621 tgcatcacaa tgaccgctac acgattcgaa agtgaggttt tggcattcag ttcctgcttt
     1681 gtctcttccc tgtcgtccag caaccggatg ctcccttctg cctgaccgca gcttctgaca
     1741 aaatggtgac tttcccgatc aatagcacta aagcggacct gtttgagtct cagcaaccgg
     1801 ttagccagtg cagtgacgtg gtgcccgtta cggtaattcg cctgcaggat attaacggtt
     1861 gtgtcgtttc cctgttgctg ccggaaaaag agacttttga gcgaactcca tgagaaaaag
     1921 ctcggatgaa ctatctgatt ggcatcacca cagagtaaaa actgaccggg atgacgtagc
     1981 gttttcagta ccagttgcag ttgaacaggc gtcatatcct gaacttcatc aacaaaaatg
     2041 acatcataac ggggagttgc caacgaaaga tactgatgac tgagcagatt actgtcatac
     2101 tgatgcgact gtttcagcca tgcgatataa cgttcaaaca ggacatagac cgtcgggcgg
     2161 tcctccatgc catagagcga ctgccgtatc cccagtgaca gatacgcctc ccgggacagc
     2221 ggcccattcc cggaggcaac cgcaccgatc acgcccctga actcctcata cagggtatgg
     2281 gcagccccca gtgcggcgat agccctgttg cggggcagcc agtcgctgaa cgcactgaaa
     2341 gtcacctccc tgccttccgg aatgcgcaga gtttccagaa attccgtcag cgaaagaaag
     2401 tcaatattct gtacctcact cccttcccct gaagcgtcat agagagtacg cgctttttcc
     2461 accagaaatg aggaaaggct gagatacagt atatcaccag cagcctgctt cattttttcc
     2521 agtaccagcg ccgtcttacc acttcccgcc gagccaacaa tcaccagcgg tagcgggtgt
     2581 tcgtacagtg cctgctgatc atcatcaaag gacagcattt tatccaggcg gtggaaacgc
     2641 ccatgagacg gattgatata cgtcagcgct tcagtcgcga tatcaacagg atccggcact
     2701 ggttgttgtt gtaagcgccc ttcatcaatc accacgtttc tgcgaagaaa acgtgaagtg
     2761 ttataggcat gattgcgaat atattccaga acaaggcaga tagtttcacc gtggtagcga
     2821 tacagcgaaa acagtaatcg gtcccggata ctgagccggg cgcggtaaag attggtgtca
     2881 attttacgaa catcagcctg aacaaagtta tcggtggcaa tagcctgtgc aaatttttta
     2941 taacccggga tcgtgtcagg attcagagta tgataaagac agattttcat ggctgccggt
     3001 ttccgtcgtg caaaaaacat gctgatttta cgaaggaatg ccttactgaa caaccttgcc
     3061 cggaataccc cccgaaatca gatggaacaa tcagaaaaat gctctgcagt tatggaataa
     3121 agaaggtggg gtggtacgcc gggagggcga tccgctggaa atggcgcagt agctgcaggc
     3181 ctgtcatgat gccggaatag aagttcgtgt acaggttaat gaaagcgtca ccccgtaaat
     3241 aaactctgcg ctgaaattcc tttttttcat tggagcacct gtgttgttct gaggtgaaca
     3301 tatcacctct gttcaggtag ccaaattcag tgtgccacct caaggttctt agtgagtcgt
     3361 ttttaaaaga tatgataata tatcattgtt tatgaattgt tgatgtttaa tttagtaaat
     3421 tatatcaaat atgccatggg tttgctatgt tattcatcca gtatctggaa ataaaatgca
     3481 ccagctttat tgggttttcc ttttgtctct gcttactagg tgccagaaac tttaaatgtt
     3541 aaacgaaggg ggcattttgt tcaaagccaa tcatgttccg gtattaatgt atcatcatgt
     3601 cagtcattgc cccggactgg ttacactttc tcccgtgaca ttccggaagc aaataaaatg
     3661 gttggctgaa aacaactgga aaacactgtc atcagatgaa ctggagtttt tttatcgggg
     3721 cggaaaactc ccccggaaaa gcgttatgtt gacatttgat gatggatacc ttgacaactg
     3781 gtttcaggta tatcctttat taaaagagtt taatctcaaa gcacatattt ttttaattac
     3841 cggttttatt gggaatggtc cggttcgtca ctctcctgga aaggaatatt ctcatcgcga
     3901 ttgcgaacat caaatagcga cagggaatgc tgataatgtt atgcttcgct ggtctgaagt
     3961 aaacgaaatg ttgcaatctg gtctggtcga atttcatgtt catacgcata ctcatacacg
     4021 ctgggataaa aagttcagtt cacgagaaga acagtgtaag catcttcggc aggacttgtt
     4081 gagcgggaga gaatatttga aagaaatgac aggaaaatgc agtaaacatt tgtgttggcc
     4141 ggaaggttat tataacaagg attatattca ggtcgctgaa gaacttggat tctattattt
     4201 atatacaaca gaaagaagaa tgaatgctcc tgctaaagga actacacgga taggaagaat
     4261 aagcaccaaa gaacgggaaa gttgcgcatg gctgaaaaga cgactttttt attatacaac
     4321 accgtttttt tcatcgttgc tggcctttca taaggggcca agattaccgg atgattaata
     4381 atgaatatac tatttacgga atcatcaccg aatataggtg gtcaggaatt acaggctgtt
     4441 gctcaaatga aggccctgaa gaaaatgggg cattcagttc tgcttgtctg cagggaaaac
     4501 agcaaaattg cttttgaagc cagtaaattg ggaattgata tcacattcgc gttatttcga
     4561 aacagtcttc acatccctac tgcatggaga ttactcggaa tagttcatgg ttttcagccc
     4621 aatgcaatcg tttgtcacag tgggcatgat agcaatattg ttggtttagt acggttattc
     4681 actaggaaac atccattcag aattatcagg caaaagacat atttgacacg aaaaacaaaa
     4741 gttttttcaa taaatcattt ttgcgatgag gtgattgttc ccggaacaag tatgaagaca
     4801 catctggagc aggaaggatg tcgaacccgg gttactgttg tgcctccagg ctttgatttc
     4861 cagaaattat acgttgattc ccgaaacagt ttgcctccaa atgttctttc ttggctggcg
     4921 tcccgaaggg gatgccccgt tattgctcag gtaggaatgt tgcgcccgga aaaagggcac
     4981 gaatttatgt tgaatttact gttccattta aaaatgaatg gacgacagtt ctgttggttg
     5041 attgtggggt ctggttcgcc tgaactgcgg gagcatttac agtatcagat tgacagtatg
     5101 ggcatgcatg atgatgtttt tattgctgac aatgtttttc ctgccgcccc cgtatatcgg
     5161 gttgccagtc tggtggttct gccttcagaa aacgaatcgt ttggtatggt actggcagaa
     5221 gcatcggcat tttctgtgcc tgtactggcc agtcagattg gtggaatccc tgatgttatt
     5281 cagaacaacc agaccgggac attgttacca gcaggtaata agcacgcatg gatgtgcgcc
     5341 ctgaatgatt tttttaatga acctgggcgt ttttatcaga tggctcgcca ggcaaaacag
     5401 gatatagaag agcggtttga tattaataaa actgcgttaa aaatactcac attagcgaag
     5461 cacaagtaac aatatgtttt ctgtaagtaa cttatcattt atcggtttcc ttaaaaggat
     5521 tgttttttcc tcagattcac tcccggggaa gtgggaacac agaaaattcc ggttcatgta
     5581 cattttgcga tgtgctataa atccggttgc cagtattcga tattattacg aactgcgttc
     5641 cttgcagtgc attgaggata ttctggcaat acaacccacg ttgccagcaa gaattcatcg
     5701 accttatttg cataaagggg ggcgagcctg gtcccggggg caatatattc ttgagcatta
     5761 tcgctttgtg caaaatttgc cagaaaaata ttctgaattt ttgtttccac aaaaatcggt
     5821 atcattagtc caattcatcg ggaaagatgg agaggatttt gatatccagt gttctcccag
     5881 tggttttgac cgggaaggag agttgatgtt gtcattgttt ttcaatcaaa tagtgattgc
     5941 ccggttaaca ttttcagtca ttctgactca aaatggacac accgctttta ttggcggatt
     6001 acagggagcc cccaaaaata ccggacctga tgttattcgg tgcgcaaccc gggcctgtta
     6061 tgggcttttc ccaaaacgga ttatttttga agcattttgt gcgttgatga aagcttgtaa
     6121 tgtctctgag tgtctggcag tcagtgagca cagtcatgtt ttccggcaat tgagatactg
     6181 gtatcagaaa cgcaaaacct ttgttgctgt ctacagtgat ttctgggagt ctgtagcagg
     6241 gaaaacctgt ggtgactggt acaaattacc aacacaggtg gttcgaaagc cgctaagcaa
     6301 tatagctagt aaaaaacgct ctgagtatcg aaaacgatac gctttgctgg attatatcca
     6361 tgaaactgct atccgctctt tggatgcata tcctgtaaac tcagaacatc aggacttaaa
     6421 ttaaggtttg atgtattctc tgaataaaat attaatgatg attttggtag gggcattcgc
     6481 actaaataat gaaaaaatac aaatccgagt ttattcctga atttaagaaa aattatcttt
     6541 cccctgttta ctggtttaca tggttcgttt tgggaatgat tgcaggtatt tcaatgtttc
     6601 ccccttcatt cagagatcct gtcttggcca aaatagggcg ttgggtgggc agattgagca
     6661 gaaaagctcg tcgcagggcg acgattaatt tatcgctttg tttcccggaa aagagtgata
     6721 cagaacggga aataattgtc gacaatatgt ttgccacagc attgcaatcc atagtgatga
     6781 tggcagaact ggcaattcgt ggtccggaaa agttccagaa acgagtgttc tggaaaggcc
     6841 tcgaaattct tgaggaaatc cggcacaata acagaaatgt gatttt
//
LOCUS       DAJVQH010000165         6802 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJVQH010000165 DAJVQH010000000
VERSION     DAJVQH010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.165"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            94..1518
                     /gene="sbcB"
                     /locus_tag="OQM87_003751"
     CDS             94..1518
                     /gene="sbcB"
                     /locus_tag="OQM87_003751"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCS1509718.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1561..1788)
                     /locus_tag="OQM87_003752"
     CDS             complement(1561..1788)
                     /locus_tag="OQM87_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCS1509719.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1802..2860)
                     /gene="yeeE"
                     /locus_tag="OQM87_003753"
     CDS             complement(1802..2860)
                     /gene="yeeE"
                     /locus_tag="OQM87_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCS1509720.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALILAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3039..4397)
                     /gene="plaP"
                     /locus_tag="OQM87_003754"
     CDS             complement(3039..4397)
                     /gene="plaP"
                     /locus_tag="OQM87_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCS1509721.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4387..4467)
                     /gene="yoeI"
                     /locus_tag="OQM87_003755"
     CDS             complement(4387..4467)
                     /gene="yoeI"
                     /locus_tag="OQM87_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCS1509722.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(4665..5594)
                     /locus_tag="OQM87_003756"
     CDS             complement(4665..5594)
                     /locus_tag="OQM87_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1509723.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(5640..6464)
                     /locus_tag="OQM87_003757"
     CDS             complement(5640..6464)
                     /locus_tag="OQM87_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1509724.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6547..6801)
                     /gene="yoeB"
                     /locus_tag="OQM87_003758"
     CDS             complement(6547..6801)
                     /gene="yoeB"
                     /locus_tag="OQM87_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCS1509725.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1839 a         1781 c         1595 g         1587 t
ORIGIN      
        1 tatttgacgt tcgtagacat actcaggtga aaaaagggtt gcttttcgag ttataacaga
       61 cagttaatta actacagtgg acaatgctag acaatgacag acacagataa acaacctacc
      121 ttcctctttc acgattacga aacctttggc acgcaccccg cgttagatcg ccctgcacag
      181 ttcgcagcca ttcgcaccga tgacgaattc aatgtcatcg gcgaacccga agtcttttac
      241 tgcaagcccg cggatgacta tttaccccgg cctggagcag tattaattac cggtattacc
      301 ccgcaggagg cacgggcgaa aggagaaaac gaagccgcgt ttgccgcccg tattcactcg
      361 ctttttaccg taccgaagac ctgtattctg ggctacaaca atgtgcgttt cgacgacgaa
      421 gtcacacgca acgtttttta tcgtaatttc tacgatcctt acgcctggag ctggcagcat
      481 gataactcgc gctgggattt actggatgtt atgcgtgcct gttatgccct gcgcccggaa
      541 ggaataaact ggcctgaaaa tgatgacggt ctaccgagct ttcgccttga gcatttaacc
      601 aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga tggctgatgt gtacgccact
      661 attgcgatgg cgaaactggt aaaaacgcgt cagccacgcc tgtttgatta tctctttacc
      721 catcgtaata aacacaaact gatggcgttg attgatgttc cgcagatgaa acccctggct
      781 cacgtttccg gaatgtttgg ggcatggcgt ggcaatacca gctgggtggc accgctggcg
      841 tggcatcctg aaaatcgcaa tgccgtaatt atggcggatt tggcaggaga catttcgcca
      901 ttactggagc tggatatcga tacattgcgc gagcgtttat ataccgcaaa agccgatctt
      961 ggcgataacg ccgccgttcc ggttaagctg gtgcatatca ataaatgtcc ggtactggcc
     1021 caggcgaata cgctacgccc ggaagatgcc gaccgactgg gaattaatcg tcagcattgc
     1081 ctcgataacc taaaaattct gcgtgaaaat ccgcaagtgc gtgaaaaagt ggtagcgata
     1141 ttcgcggaag ccgaaccgtt tacaccttca gataatgtgg atgcacagct ttataatggc
     1201 tttttcagtg acgccgatcg tgcagcaatg aaaattgtgc tggaaaccga gccgcgtaat
     1261 ttaccggcac tggatatcac ttttgttgat aaacgaattg aaaagttgtt gttcaattat
     1321 cgggcacgca acttcccggg gacgctggat tatgccgagc agcaacgctg gcaggagcac
     1381 cgccaccagg tcttcacgcc agagtttttg cagggttatg ctgatgaatt gcagatgctg
     1441 gtacaacaat atgccgatga caaagagaaa gtggcgctgt taaaagcact ttggcagtac
     1501 gcggaagaga tcgtctaatt tgaagccgcc gccgctggta cggcggagga caatgttgag
     1561 tcaggctttt tgaacggtga tgctccacgc tgcatcgcca atctgctgat aatcggtgat
     1621 ggcatgtcct tcttctgccg cccactgtgg gattgcttca gttgcctggg tacaatcaaa
     1681 ctcaattacc aactcatcgc cgctggccat ttctgccagt gctgcttttg cttctatgag
     1741 tggaaacgga cagacctgcg tcaccacatc cagcttttta atcgccatct ttacacctta
     1801 cttaatttgc cgcagcagtt gccagtcgcg ccttacgctg cggtcgaaca tacacaagcc
     1861 aggacgcagt ccagactccg agaatcataa atacaaggcc aatccagccc tgccaggtca
     1921 tcattgcagt cataaccagt ccattaccga tagagcaacc acctgcaata ctggcaccga
     1981 agcccatcag tacaccacct aacccgctac gtaatgttgt ttgtgcatca gctgcgcgaa
     2041 cgcggaactc acggctcgct ttggctgcaa taaatgcccc cacgaagatc cctaacacca
     2101 ggaaaacgcc ccagttaatg tatttcacgt cacccgcgac cagaaattgc agaatattgg
     2161 ccgttgggga agtgactcct aacccaaaca tgcgtccggt tgcttcactc aggggccagg
     2221 ctaaaagcgc aatcaaaccg atgagtacag cagtgacaaa gggatgccag cgtttttcaa
     2281 acagaatatg agcgatcccg gttcggcgcg gcggtaaggt cgcgactttg agttttggct
     2341 tcttcaactc tttcatcacc acccagagcg ttatcaccag caaaacggta accaacggcc
     2401 acacagacaa attaaaagtc tcagcaatag agttatgttc agtagtgtag tgctgcaagg
     2461 tttgatttaa accactggcg tgtggagaac gcatcaccgc actcatcacc atataagtga
     2521 aaagcgcgat ccaactgccg atcaatccct cacccgcgcg ataccaggtc ccggtggcac
     2581 atccacccgc cagaacaatt cccagcccga aaagataccc acctataaca gtaccaagcc
     2641 acgggaacgc cccggcttcg taagtcagta ggcccgccag aattaacgca aaaaccccca
     2701 cgctttgtgc cgaaattgca atcagcaagg cgtaaaacat gcgattattt ttcgcgatat
     2761 acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg ctgcatgaca aatcccagca
     2821 gagcaccaca aattagcccg cttaatatca ttgaaaacat aaattaatta accagatgaa
     2881 tgttaatggg gaaatttttc atgactggtg gaatgcagac caataaccaa attctctaaa
     2941 ttagaacaaa tggttattaa tgaggatttc gataaaaaag aataaaaaaa ccggagccaa
     3001 atgttcattc gactccggtt tattattaga aaagatgatt atgctacgtc ttcgtactgc
     3061 ggaaccggat tacggaagct cttggtgacg caagccaggt aaatcagacc gatagcagcc
     3121 cagatcagac ccagaaccat tgagctttct tccaggttaa cccacagcgc accaacggtc
     3181 agcgcaccac acatcggcag gaacagatac tggaagtgat ctttcagcgt cttgttacgc
     3241 ttctcacgga tccagaactg cgagatgacc gacaggttaa caaaggtgaa cgccaccagc
     3301 gcaccaaagt taatcagcgc cgttgccatt accaggtcga agttgattgc cagcagggca
     3361 atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc atttcgggtg tacataaccg
     3421 aagaagcttt tcgggaacac gccgtcacga cccattacgt acatcagacg cgctacgcct
     3481 gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg agaagatcag cgcgccaacc
     3541 tggaacgctt tacccgcaac gtacagcatg atttcaggct gtgacgcatc cggatcttta
     3601 aagcgagaga tatccgggaa gtacagctgc aggaagtaag ttgcaaagat gaagatcatg
     3661 ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca cgcgctctgc atctttggtt
     3721 tcttccgaca gattgctgat gccgtcaaag ccggtaaagg agaagcacag gattgtcgcc
     3781 ccggtaatca tcgggataac atgcgcatcg ccagaccaga acggacgcgt gctcgccagc
     3841 gtaccagcgc cttcaccttc aaatacgcca taaacaacca tgcccagaat caccgcgatc
     3901 agcactacct gcaacacgac gattacagtg ttgaagttcg ctacggattt cagactacgc
     3961 aggttaaagg cggtcataaa ggccaccagc gccaccacaa acatccacga tggaatggaa
     4021 ggcaccagag cttcaaaata gattttcgcc agcagaatgt tgatcatcgg cgcgaacaga
     4081 tagtcgagca gagaagacca acccaccata aagccgacag tcgggctaat ggatttctgg
     4141 gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa ccagcttccc gtagctcaga
     4201 gccgtaaaca ggatcgcaat caatgcgaac gcataggctg tcggcacatg accatccgtg
     4261 aggcctgaaa cgataccaaa tgtatcaaac agcgtcatcg gctgcatata ggctagaccc
     4321 atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt ccacgcgaga ggtgtttgga
     4381 gtaacgttat gcgacatggt tattctcctt tacggtgata accgtcgcgt aagcaaaaaa
     4441 tttgccccat ttttttggat tcctcagcga caacaactgt cgatttttag taaatatcta
     4501 tccggtacga agcccggcct cttggtatga atgattggtt tgaagcaaaa aataaccgac
     4561 gctaattaaa cgtcggtttt tagtcatttt ttgacagcgg cgcattgtgc ctaaatgggg
     4621 gggaaatgac aagagaatga gaggcttgtc ataataattt ttctttaaat ggctgatttt
     4681 ccgtcatcag attttgcata aacaccggcg atcgcactat ttgctaaaat ttcatctcgc
     4741 caccagccgc tggcaagccc tgcggtctgt tcattccagc caatccagac aggttcaaag
     4801 agagtttggg cgaccacttt cttctcgatt aacgcgccac tatcgagaaa acgttgcgcc
     4861 agataccgtg gtaaatagcc gcagccaagg ccgctaattt gcagctccag tttggtttta
     4921 aaatcgaaaa cggtaatggc ttcttgttcg tcgagaagtt gtgaggccgt agaagcgcct
     4981 gcctgcgcag tgtcccccac cacaatcgcc cgatatcgtt taatgatacg acggtttaac
     5041 ggttcttctt cctgggctaa cggatgatga ggcgcgaccg caaagacttg ttcaagatcg
     5101 cccaaccgcg agaaaccaaa ctcactggaa gatggtggct catgcatcgc gccaacgata
     5161 atatccgctc gcccctgagt cagggcatcc caggaaccac cgagtacgcc attgataaac
     5221 ttcaggcgcg tcacgctatg atgttgataa aaagcttcaa tgagaggcgc aagcagagaa
     5281 aaaggaaacg tgtcgtccac gccaatcacc agctcgtttt cccagccttc atgcagtttg
     5341 atcgcctgct tttccagttc tcgcacggta tgcagaactt cccgcccttt ctctaataac
     5401 atttttccgg tgcgggtgaa tttagcgcgg tgtccgctac gatcaagcaa ttgaatattt
     5461 aggtcgcttt ccagcttgtg aacggtgtaa ctcagagcgg acggcgtctt gtagagtttg
     5521 gctgacgccg ccgcaaaact gccctctttt tctatggcat cgaggatcat caacacatcc
     5581 agcagtggtt tcatacacgc ccccttgcag tgcatgcggc gtcctgctgg cgtgatcgtt
     5641 tactccagcg gcattaccag cggatcggga tactggtatt caaatcccag ttcattacaa
     5701 atccgactgc catcaataat cttacctttg ccgctgtcca gactatttct gaactgcgga
     5761 ggttgcagcc ccagtaaacg ggccatctgc ggatagaaaa cattacgcgc agggtgagct
     5821 ggcgcacata tattatagat gtgtccgcct ttaggtgcct gtaacaacag agtgatagcg
     5881 ccaataacat cttctaaatg gactaaatta acaccatgtt caccatcagg ggcggttttt
     5941 ccggcaaaga agcgtcccgg atgacgtccc ggtccgacca ggcccgcaag acgcagaata
     6001 tcgaccgaag taccgggtaa attgtgcagc cagtcttcga gttcttctaa tactcgtcca
     6061 ctgttggtta ccggattacg cggggtagtt tctttaaccg tgccttgcgc gtcgccatag
     6121 acagatgttg agctggtaaa aataattcgg ggaatacgat gggccagcgc gctatccact
     6181 aactcttgta ccgcttgtaa atagaactca tcgccggggc cgctacgacg tgccggaagc
     6241 gtaatgacca gcgcatcggc atccataagg gcatccagat cgtcagaatc gcaaactaac
     6301 tcaggctcca tgcgaagcag atagctatca atgccactca ttcgggccgc ttcgacacca
     6361 tcttgtgtgg ttttactccc ggtgacttgc cagcctcgcg ctgaaagtga catcgccagc
     6421 ggcataccta accaccctaa cccgacaatt gcgacctttt tcatccgtta tctcctgata
     6481 caccttagat ctataaggct acgctagcgt atcaaaactg acaattcatt ctatgaatga
     6541 atctgttcaa taatgataac gacatgctgc aatgagcagt gaatcatcag taaccgcgta
     6601 taccagacgg tgctcctctg taatgcgtcg ggaccagaaa cctgacaaat tatgtttcag
     6661 gggttctggt ttccccttac cttcaaatgg tgttctgcgg gtatctttga taagttcatt
     6721 gatcttttta acaattcgct tatctgtttc ctgccagtac agataatcat cccatgattc
     6781 ctcagaccag attagtttca ct
//
LOCUS       DAJVQH010000166         6770 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJVQH010000166 DAJVQH010000000
VERSION     DAJVQH010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.166"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..3513
                     /locus_tag="OQM87_003759"
                     /pseudo
     CDS             1..3513
                     /locus_tag="OQM87_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3523..3766
                     /locus_tag="OQM87_003760"
                     /pseudo
     CDS             3523..3766
                     /locus_tag="OQM87_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            5305..6770
                     /locus_tag="OQM87_003761"
                     /pseudo
     CDS             5305..6770
                     /locus_tag="OQM87_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363073.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1794 a         1779 c         1629 g         1568 t
ORIGIN      
        1 cgaaagataa ttctttgcaa ccttcccaaa cggtaacgga ctctctcaag gtagcggact
       61 tatccaccat tattcaggaa caaccctctt tcgtagcaga aacccgtgcg gctgacaaaa
      121 atacagatgc tgttttacct tggctggcga aagatattgc tgtcgttttt ccgcccgaag
      181 ttgtacacac cacgttaagt catcgccgct ttcccggagt tcctgttcag caagcagaca
      241 aattgaccca attacgtcgc ttagcctgta gcgtgtcgca acgggataat aaaacagcca
      301 catttgactt cagcacctgt tctttagaat ggcaaaacac cgtcgcccaa gcgatcagtc
      361 agatcgacgg cctgaaaaca acacagctac catcaccagt aatggctgta ctcacggcac
      421 ttgaaatgaa atgcacaaga tataaagtgc gtgaggatgt tatggatcag atcgtccagg
      481 aaggcggtct ggaatatgct actgatgtaa taatacacct tcaacagatt gatattaaat
      541 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
      601 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      661 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      721 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      781 gtctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      841 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      901 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      961 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
     1021 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
     1081 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agaacaggtc attccctggc
     1141 tttcgacacc cgcagttgca gtgctgaaat catgccagca ccaactgaca cagccctcaa
     1201 accatgccag cgccgatcta ctgccagcca tagtagtctc cccaccatgg atttcgaaaa
     1261 agaaaaaatc gccgattccg gtgctggatt tagcgccatt aggcatcgag ccaatctgtt
     1321 atctgacaga agaaatcagt aatcaacttt tggcgaaata tatctggtat tcaaaacaca
     1381 tcacggttag ccatgaagaa agtactacca acctgttggc aaggatgggt tttcaacgac
     1441 ggatcgctgg tacatatatt aaagctcccg aagcggtagt tgaggcatgg ctaaatgaag
     1501 attattcaac cttactaagt gaatttaagg tgtttcattc acctaccggg cattattggc
     1561 agttggggat tttgacaaca ttgccgctgg aggctggaga aagcagtaaa agcatggaat
     1621 gcccttactc tatctccaca taccgatacc gaatacgcca tgttacattt tggactcaaa
     1681 ggtttacctg gtttagttaa ctcaattaca cgctatccgc aggaagcctt gcccatcacg
     1741 aattacttcg cagcgagtga gctggctcct gccgtcgccc gtgccttcaa caaactcaaa
     1801 acgctacgcg aaaacgcccg cacctggctg ttgaaatacc cggaacatgc ccttaccggc
     1861 ctgctgcctg cggcgctcgg caaagccggt gaagcacaag ataacgcccg cgctgccttg
     1921 cgtatgctta ccgaaaacgg tcatcagcca ttactgcaag aaatcgcccg gcgttataac
     1981 cagtcggaag taacagacgc ggtgaacgct ctgcttgcgc tcgatccctt agataatcac
     2041 ccgacaaaaa tccccactct tccgaccttt tatcagccat cgatctggac gcgcccggta
     2101 ttaaaagcaa acgcccaatc actgccagat aacgccctcc tccacctcgg cgaaatgctc
     2161 cgcttccctc aggaagaggc tctgtatccg ggattattgc aggtgaaaga cgcctgtacc
     2221 gctgactctc tggctgaatt tgcctgggat ctgtttaccg cctggcagac cgctggcgcg
     2281 ccgtcgaaag agagttgggc gttcactgcg ttaggcgttc tcggtaacga tgacaccgcc
     2341 cgcaaactga cgccattaat acgcgcctgg cctggtgaat cccagcataa acgcgccacc
     2401 gttgggttgg atattctcgc cgctatcggt agtgatatcg ccctgatgca gcttaacggc
     2461 attgctcaga aactgaaatt caaagcatta caggagcggg caaaagaaaa gattgccgac
     2521 attgccgaga gccgcgaact cacggtagcg gagcttgaag atcggttagc accggatctc
     2581 ggtctggatg ataacggttc gttgctgctg gattttggcc cacgccagtt caccgtcagc
     2641 tttgatgaaa ccttaaaacc gtttgtgcgt gatgtttccg gcagccgcct gaaagacctg
     2701 ccaaaaccaa acaaaagcga tgatgagtca caggcgaacg atgctgttaa ccgctacaaa
     2761 ttgctgaaaa aagatgcgcg taccgtcgcc gcccagcagg tagcaaggct tgaatccgcc
     2821 atgtgcctgc gtcgccgctg gtcgccggaa aacttccaac tcttcctggt tgtgcatccg
     2881 ctggttcgcc acttaacccg ccgtctgatt tggggcgttt atagcgccga caaccagcta
     2941 ctggcctgtt tccgggtagc ggaagataac agctacagca ccgctgacga tgatcttttc
     3001 accctgccgg aaggcgatat ctctatcggc attcctcacg ttctggaaat atcaccgacg
     3061 gatgctgtcg cctttggtca gctttttgcc gactacgaac tgctaccacc gttccgtcag
     3121 cttgaccgta acagttacgc cctgacagaa gccgagcgca acgccagcga actgacccgc
     3181 tgggcaggca gaaaatgccc gagcggccgg gtcatggggc tggcaaataa aggctggata
     3241 aagggcgaac cacaggatgg aggctggatc ggctggatga tcaaacctct cggtcgctgg
     3301 tcgttaatca tggaaatcga cgaaggtttt gcggtgggta tgtcaccagc cgaacttagc
     3361 gctgagcagc tcttaagcaa gctgtggcta tgggaaggca aagcggaaag ctatggctgg
     3421 gggagtaatt caacacagga agcgcagttc tccgtactcg atgccatcac cgccagcgag
     3481 ctaattaacg atattgaagc cctgtttgaa taaggaaaga gcatggacaa ggaattaccg
     3541 tggctggcgg ataacgccca actggaactg aaatataaaa aaggcaaaac accactcagt
     3601 catcgcaatt ggccgggcga accagtgcct gttatcactg aaagcatcat tcagacactg
     3661 ggtgatgaat tgctacaaaa agctgagaag aaaaaaaaca ttgtctggcg ttatgaaaat
     3721 ttttcaccgg agtggcagtc cgccattacg caggccatca acttgatcgg cgaacacaaa
     3781 ccctcagtcc cggcccgcac aatggcggca ctagccttta tcgcgcaaaa tgacaaccaa
     3841 tagttgctcg acgaaatcgt ccaacaagag gggctggaat atgcgactga ggtggtgatt
     3901 gcacgccagt ttattgcacg gtgttatgag agtgatcctc tggtagtcac attgcagtat
     3961 cagaacgagg actatggcta tggttatcgc tcagaaacct ataacgaatt cgatctccga
     4021 ctgcgtaaac acctctctct ggcagaggaa agctgctggc agcgttgcgc cgacaaactc
     4081 attgccgcac tgccaggaat aaccaaagtt cgccgccctt ttattgcgct gatcctcccc
     4141 gaaaaaccag aaatcgccaa tgagttggta agccttgaat gcccgcggac tcattttcat
     4201 tctaaggagt ggttaaaagt tgttgctact gaccccaaag cggtgagaaa actcgaacgc
     4261 tactggagcc aggatatatt tagcgatcga gaagccagct acatgtcgca tgaaaaccac
     4321 ttcggctacg cggcctgcgc tgcccttttg cgcgaacaag gactggcagt cgttccgcgc
     4381 ctcgcgatat atgcccataa agaagattgc ggcagtctgc tggtacaaat taaccatccg
     4441 caagtcatcc gcaccttgct gttagttgct gataaaaaca aacccagcct gcaacgtgtg
     4501 gctaaataca gtaaaaactt cccccatgcg acactcgccg cactggcaga actgctggcg
     4561 ttaaaagaac caccagcccg cccaagttat ccaatcatcg aagacaaaaa gctgcctgca
     4621 cagcaaaaag ctcgagatga atactggcgt acgctgttac aaacgctgat ggcatcgcag
     4681 ccacaactgg cagcagaagt gatgccgtgg ttaagtactc aagcccgggc agtgctgaat
     4741 agttatttat cggcaccgcc caaaccagtt attgatagca ccgataacag ccaaatgcct
     4801 gaaatcctcg tttcaccacc atggcgtggg agaaagaaaa agacttccat acgtcacttt
     4861 tcgctgcctg atcttacgct tgcaccgcaa acgtacaggt cgccgaataa acactggacg
     4921 ccggaacagc aaactgcaat tgaccatttt agcgccatgt cttttgacga acgtcttgct
     4981 acgagaggag cggatatctt cttgcgggaa ttaggttttg acgttcatag cctggaattt
     5041 aataagcgtc ttcttaatgg acagttggat gcctttgatg ctcagcaact gcaagaatac
     5101 acatcaatag gactattgcg tacttatcac gcgcatcagg ccaaatctaa acagtaccgg
     5161 gaagacgcca ccaatgcatt actggcacaa gatagcattg cgttgatggg agggtggcga
     5221 acttttcagc acggatttta ctatggtaat gaacagcgtt gggggatttg gaatctgtac
     5281 cttatcgcgc agatgccgcg cgaaatggcg gtatcgtgct ggcaacaaat tgtcgaggct
     5341 gattttcatt acaccggggt ggaatatttg ttaagtgtgc tgggtaccga tgctttgcct
     5401 ggattaaaca ccgcctttgc ccgacatcca aaagagatat ttccgctgct gattcatttc
     5461 ggcgcaacag aactggccct gcccattgcc cgcgtctggc acctttttgc gggccagcgt
     5521 aatctggctc gccagtggat tttacaatgg ccggaacata cggctactgc acttatccct
     5581 cttgtcttca ccaaatctag cgataaaagc gaagctgcat tacttgccct gcgtttactt
     5641 tacgaacatg ggcatggcga attgctacaa actgtagcca atcgttggca gcgtacagat
     5701 ctatggcctg ccctggaaca attacttaaa cagagtccaa tagagattta cccggcacgc
     5761 attccaaaag cccctgattt ctggcatccg caaatgtggt ccaggccgcg ccttatcact
     5821 aataatcaac ctgttaccga tgacgctctg gaaattatcg gcgaaatgct gcgctttacc
     5881 caggggggac gtttttatag cgggctggaa caactgaaaa cgttctgcca gccacaaacg
     5941 ctggcagctt ttgcctggga tctcttcact gcgtggcaac aagctggtgc ccccgcaaaa
     6001 gacaactggg catttctggc gttaagtctc tttggtgacg aaagcacggc acgggatcta
     6061 acgacacata tcctcgcctg gccacaagaa ggcaaatctg cccgtgcggt cagtggcctg
     6121 aacatcctta ccctgatgaa taatgatatg gcgctgatgc agctgcatca tatatcgcaa
     6181 cgggcgaaat cccgcccctt acgtgataac gcggcggaat ttcttcaggt agtcgcagaa
     6241 aatcgcgggc taagccagga agagttagcg gacagattag tcccaacact gggccttaat
     6301 gatccgcagg cgttgagttt tgattttggt ccccggcagt ttaccgttcg cttcgatgaa
     6361 aacctcaacc cggttatctt tgatcagcaa aacgttcgcc agaaaagtgt tccccggttg
     6421 cgcgccgatg acgatcaact gaaagcgccc gaggcactgg cccgactaaa agggttaaaa
     6481 aaagatgcta ctcaggtgag caaaaacctg ctcccgcgtc ttgaagctgc cctacgtacc
     6541 acccgacgct ggtcgctggc agattttcat tctctgtttg ttaatcatcc ctttacccgt
     6601 ctggttaccc agcgattaat atggggggtt atccggcaaa tgaaccgcgt cgtttactca
     6661 acgcctttcg tgtggccgca gagggggagt tctgcaatgc gcaagatgag ccaattgacc
     6721 tgcctgcgga cgctctgatt ggcattgccc acccgttaga aatgacagca
//
LOCUS       DAJVQH010000167         6705 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJVQH010000167 DAJVQH010000000
VERSION     DAJVQH010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.167"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..756
                     /locus_tag="OQM87_003762"
     CDS             <1..756
                     /locus_tag="OQM87_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1509726.1"
                     /translation="SGSSHQLGLSGTALERNNLSWNLMQSYNSHDGEVGGNMSLTYDG
                     SYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGLEIDN
                     MRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEF
                     VTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQVTL
                     SWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            767..1840
                     /locus_tag="OQM87_003763"
     CDS             767..1840
                     /locus_tag="OQM87_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1509727.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSSLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            2088..3128
                     /gene="pstS"
                     /locus_tag="OQM87_003764"
     CDS             2088..3128
                     /gene="pstS"
                     /locus_tag="OQM87_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCS1509728.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLEKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            3215..4174
                     /gene="pstC"
                     /locus_tag="OQM87_003765"
     CDS             3215..4174
                     /gene="pstC"
                     /locus_tag="OQM87_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCS1509729.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            4174..5064
                     /gene="pstA"
                     /locus_tag="OQM87_003766"
     CDS             4174..5064
                     /gene="pstA"
                     /locus_tag="OQM87_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCS1509730.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            5156..5929
                     /gene="pstB"
                     /locus_tag="OQM87_003767"
     CDS             5156..5929
                     /gene="pstB"
                     /locus_tag="OQM87_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCS1509731.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPELRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5944..6669
                     /gene="phoU"
                     /locus_tag="OQM87_003768"
     CDS             5944..6669
                     /gene="phoU"
                     /locus_tag="OQM87_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCS1509732.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGSDELDKLLAGKDSDK"
BASE COUNT         1690 a         1571 c         1746 g         1698 t
ORIGIN      
        1 tccggtagct cacatcaact cggactcagc ggtacggcgc tggaaagaaa taacctttca
       61 tggaatttaa tgcagtccta taacagtcat gatggtgagg ttggcggtaa tatgtccctg
      121 acctatgatg gctcttatgg cacggtgaac ggcagctata actacagcca aaattcccag
      181 aggctgaatt atggtatcag agggggaatt ctggcacaca gcgaaggggt aacgttaagt
      241 caggagttag gtgaaactat tgctcttgtt aaagcacctg gggccgccgg gttagaaata
      301 gataatatgc gcggtgctgc gacggactgg cgcggctata cggtcaagac acagctaaac
      361 ccttatgatg aaaatcgggt agcaatcagc gataactatt tctcgaagtc gaatatagaa
      421 cttgataata ccgtcgttac gatggttccc acgcgtggtg cagtggttaa agcggagttt
      481 gtgactcatg tgggttatcg cgttctcttc agagtgttaa atgcaaatgg taaaccggta
      541 ccttttggag ccattgctgc gatacaagat gcaagtttgg cagattcagg aattgtcggt
      601 gaccgtggcg aactttatct ttctggtcta ccagaaaaag gacaggttac gttatcctgg
      661 ggagaaaacg cctcaacaaa atgcatcttc aattattcac tttcgacacc agaaagtgag
      721 agcggattaa ttgaacaggg tgtgacatgt cattaaaagg atttttatga acaagtatat
      781 caaacagtgg tgcttttctg tgtttatgct ctcgttaagt agtgtagcac ttgcagctcc
      841 taaaggtatc tgtacccctg ataatggcgt atttcacagt tcgcttgatt tctcgggata
      901 tcttattacc gcgaacgaaa ataaggttgg aacgacattt aacaccactg taactaatgg
      961 atcctcatat cttggacgct gtcattgtga tactggtaac gtaggagaat tcccctatat
     1021 ctattatacg tcaaaaataa accaggcatt aacttatgct ggggttcact ctaatattaa
     1081 ttattatgat cttaatccga accttgatgt tgggattgct atagatattc ttggggttgg
     1141 gtatgtgaat gcaccttttg aatatcatgc taacaacccg tctggtaata caaagtacaa
     1201 ttgcaaccgc attgaacctt taagtatatc cagtggggct aaagcgatag tatattttta
     1261 tatcaagaag acatttgcag gaaaattgat tattcctgaa acaaagatag tgacattgta
     1321 tggaacaatt agccgtgaca ccccggtgga ttactcacag ccgatggctg atgtttatat
     1381 tcggggcgat attacagctc cgcaaagttg cgaaataaat aatttaaagc cagtttgttt
     1441 tgattttaaa gaaataccag ctgcagattt ttcatctgtt gttggaagtg cggtaacaac
     1501 acataaaatt actaaaacag tcactattga gtgtgaaaac ctagggatac tgaataccga
     1561 tgatatcagt acgtcttttt atgctaccga acccaatacg gacaattcaa tggtcgtaac
     1621 ttcaaactct aacgtgggga taaaaattta cgacaaaaat aataaggaaa tcaaagtgaa
     1681 cggtggtgag ttgccaacag acatggataa atcaacagtg tatggtgaaa aatcaggtag
     1741 tgtaactttt tcagctgctc ctgcgagcct tactggagct cgcccagccc cggggcaatt
     1801 tactgcaaca gcgacgataa ctgttgagat tgtgcgctaa taggtctgac aacgattgtt
     1861 catcacatcg ttgtcatctc acccatttct ttatcacttt taaatgcatc atgacaaaaa
     1921 gatggcatgt gggattttca tcttcacatt taaaatcagt aagttatttg gtaattcctt
     1981 tctgtcataa aaatgtcata ttccttatat ataactgtca cctgtttgtc ctattttgct
     2041 tctcgtagcc aacaaacaat gctttatgaa tcctcccagg agacattatg aaagttatgc
     2101 gtaccaccgt cgcaactgtt gtcgccgcga ccttatcgat gagtgctttc tctgtgtttg
     2161 cagaagcaag cctgacaggt gcaggtgcaa ccttccctgc gccggtgtat gccaaatggg
     2221 ctgacactta ccagaaagaa accggtaata aagttaacta ccagggtatc ggttcttccg
     2281 gtggcgtaaa acagattatc gctaataccg ttgattttgg tgcctctgac gcgccgctgt
     2341 ctgacgaaaa actggctcag gaaggtctgt tccagttccc gaccgtgatt ggcggcgtgg
     2401 tgctggcggt taacattcca gggctgaagt ctggcgaact ggtgctggat ggtaaaaccc
     2461 tcggcgacat ctacctgggc aaaatcaaga agtgggatga tgaagccatc gccaaactga
     2521 atccgggtct gaaactgcct tcacaaaaca ttgctgtagt acgccgcgca gatggctccg
     2581 ggacttcctt cgtcttcacc agctacctgg agaaagtgaa cgaagagtgg aaaaacaacg
     2641 ttggtactgg ctctaccgta aaatggccga tcggtctggg cggtaaaggt aacgacggta
     2701 tcgccgcgtt cgttcagcgt ctgccgggtg caattggtta tgttgaatat gcttacgcga
     2761 agcagaacaa cctggcgtac accaaactga tctccgctga tggtaaaccg gttagtccga
     2821 ccgaagaaaa cttcgctaat gcagcaaaag gtgcagactg gagcaaaacc ttcgctcagg
     2881 atctgaccaa ccagaaaggc gaagatgcat ggcctattac ctctaccacg ttcattctga
     2941 tccacaaaga tcagaagaaa ccagaacaag gcacagaggt gctgaaattc ttcgactggg
     3001 cgtacaaaac cggggctaaa caggcgaacg acctggatta cgctagcctg ccggatagtg
     3061 tagttgaaca ggttcgcgct gcgtggaaga ccaatattaa agacagtagc ggtaagccgc
     3121 tgtactaata aaactccagg ccgggtacgg tgttttacgc cgcatccggc attacaaaat
     3181 gactttgtaa acgcgtttaa ctgaagagta acttatggct gcaaccaagc ccgcttttaa
     3241 cccaccgggt aaaaagggcg acataatttt cagcgtgctg gtaaaactgg cggcgctgat
     3301 tgtgctattg atgttgggtg gcattattgt ctctctgatc atctcctcct ggccgagcat
     3361 tcagaaattt ggtctggctt tcctatggac caaagagtgg gatgcaccga acgatatcta
     3421 cggggcgctg gtgccgatct acggtacgtt ggtgacttcg tttatcgcgc tgctgatcgc
     3481 cgtcccggtg agtttcggta tcgccctgtt cctgactgag cttgcgcctg gctggctgaa
     3541 acgcccgctg ggtatcgcca ttgagctgct ggcagccatt ccaagtatcg tttacggcat
     3601 gtggggcctg tttatctttg cgccgctgtt cgccgtttac tttcaggagc cggtcggcaa
     3661 tatcatgtcg aatatcccga ttgttggcgc gctgttctct ggccccgcat ttggtatcgg
     3721 tatcctcgcg gcaggcgtga tcctcgccat catgattatt ccgtacattg cggcggtaat
     3781 gcgtgatgtg ttcgaacaaa ccccggtgat gatgaaagag tcggcctacg gtattggctg
     3841 caccacctgg gaagttatct ggcgtatcgt tcttccgttc accaaaaatg gtgttatcgg
     3901 cggcatcatg ctggggctgg gccgcgcgct cggtgaaacc atggcggtga cctttatcat
     3961 cggtaacacc taccagctcg acagcgcctc gctgtacatg ccgggcaata gtatcacctc
     4021 tgcgctggcg aacgaatttg cggaagcgga atccggtctg cacgttgccg cactgatgga
     4081 actgggcctg atcctgtttg tgattacctt catcgtcctc gccgcatcga agtttatgat
     4141 tatgcgcctg gctaagaatg agggggcacg ctaatggcta tggttgaaat gcaaaccact
     4201 gcggcgctgg ctgaatctcg ccgcaaaatg caggcgcgtc gccgcctcaa aaaccgtatt
     4261 gcgctgacgc tctcgatggc gacgatggcc ttcggcctgt tctggctgat ctggatttta
     4321 atgtccacca tcactcgcgg tatcgacggt atgtcgctgg cgctgttcac tgaaatgacg
     4381 ccgccgccca atacggaagg tggtggtctg gcgaacgccc tggcgggtag cgggctgtta
     4441 attttgtggg ccacggtatt cggtacgccg ctgggcatta tggcggggat ttatctggcg
     4501 gaatatggtc gaaaatcctg gctggcagaa gtgattcgct tcattaacga cattctgctc
     4561 tccgcgccgt cgattgtggt tggtctgttt gtctacacca tcgtggtggc acagatggag
     4621 cacttctccg gctgggcggg cgtaattgcg ctggcgctgt tgcaggtgcc gatcgttatc
     4681 cgcaccaccg agaacatgct gaaactggtg ccggacagcc tgcgtgaagc ggcttatgcg
     4741 ctgggtacac cgaagtggaa gatgatctcc gcgattacgc tgaaagcgtc ggtgtccggg
     4801 attatgaccg gtatcctgct ggcgattgcc cgtattgccg gtgaaaccgc gccgctgctg
     4861 tttaccgcgc tctccaacca gttctggagc acggacatga tgcagccgat cgccaacctg
     4921 ccggtgacga tctttaagtt tgcgatgagc ccgtttgcgg aatggcaaca actggcctgg
     4981 gccggggtat tgatcattac cctgtgcgtc ctgctgctga acattctggc gcgcgttgtt
     5041 tttgcgaaga ataaacacgg ttaacacttt gccggatgcg gcgtgaacgc cttacccggc
     5101 cctacggtaa gcctgattag cgaaaagcat caggtacgat gaggaaaaga ttgcaatgag
     5161 tatggttgaa actgccccga gtaaaattca ggttcgtaat ttgaacttct actacggcaa
     5221 attccatgcc ctgaaaaaca tcaacctgga tatcgccaaa aaccaggtta cggcgtttat
     5281 cgggccgtcc ggctgcggta aatcgacgct gctgcgtacc ttcaacaaaa tgtttgaact
     5341 gtacccggag ctgcgtgcgg aaggtgaaat tctgcttgat ggcgacaaca tcctgaccaa
     5401 ctctcaggat atcgccctgc tacgtgcgaa agtgggcatg gtgttccaga aaccgacgcc
     5461 gttcccgatg tccatctacg acaacatcgc ctttggcgtt cgtctgtttg agaagctctc
     5521 ccgtgccgac atggacgagc gcgtgcagtg ggcattgacc aaagccgcat tgtggaacga
     5581 aaccaaagat aaattgcacc agagcggtta ctctctctct ggtggtcagc aacagcgtct
     5641 gtgtattgcg cgtggtatcg ccattcgccc ggaagtgctg ctgctcgacg aaccgtgttc
     5701 ggcgctcgac cccatctcta ccgggcgtat tgaagagctg atcaccgaac tgaagcagga
     5761 ttacaccgtg gtgatcgtta cccacaacat gcagcaggct gcgcgttgtt ccgaccacac
     5821 ggcatttatg tacctgggcg aattgattga gttcagcaac acggacgatc tgttcaccaa
     5881 gccagcgaag aaacaaacag aagactacat caccggtcgt tacggttgat tcaggagtgc
     5941 gttatggaca gtctcaatct taataaacat atttccggcc agttcaacgc cgaactggaa
     6001 agtatccgca cgcaggtgat gaccatgggc ggcatggtgg agcagcagct ttctgatgca
     6061 atcaccgcga tgcataacca ggacagcgat ctggcgaagc gcgtgatcga aggcgacaag
     6121 aacgtcaaca tgatggaagt ggcgatcgat gaagcctgcg tgcgcattat cgccaaacgt
     6181 cagccgacgg cgagcgacct gcgactggtt atggtgatca gtaaaaccat tgccgagctg
     6241 gagcgtattg gcgacgtggc ggacaaaatc tgccgtactg cgctggagaa attctcccag
     6301 cagcatcagc cgttgctggt aagtctggag tcgctgggcc gtcataccat ccagatgctg
     6361 cacgacgtgc tggacgcgtt cgcgcggatg gacattgacg aagcggtacg tatttatcgt
     6421 gaagataaaa aagtcgatca ggaatacgaa ggtattgttc gtcaactgat gacctacatg
     6481 atggaagatt cgcgcactat tccgagcgta cttaccgcgc tgttctgtgc gcgttcaatc
     6541 gaacgtatcg gcgaccgctg ccagaatatt tgtgagttta tcttctacta cgtgaagggt
     6601 caggatttcc gtcatgtagg cagtgatgag ctggataaac tgctggcggg aaaagatagc
     6661 gacaaataat tcaccagaca aatcccaata acttaattat tggga
//
LOCUS       DAJVQH010000168         6591 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJVQH010000168 DAJVQH010000000
VERSION     DAJVQH010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.168"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            288..437
                     /locus_tag="OQM87_003769"
     CDS             288..437
                     /locus_tag="OQM87_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509733.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            638..2221
                     /locus_tag="OQM87_003770"
     CDS             638..2221
                     /locus_tag="OQM87_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1509734.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2673..4526)
                     /gene="asmA"
                     /locus_tag="OQM87_003771"
     CDS             complement(2673..4526)
                     /gene="asmA"
                     /locus_tag="OQM87_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCS1509735.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4548..5129)
                     /gene="dcd"
                     /locus_tag="OQM87_003772"
     CDS             complement(4548..5129)
                     /gene="dcd"
                     /locus_tag="OQM87_003772"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCS1509736.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5221..5862)
                     /gene="udk"
                     /locus_tag="OQM87_003773"
     CDS             complement(5221..5862)
                     /gene="udk"
                     /locus_tag="OQM87_003773"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCS1509737.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            6180..>6591
                     /locus_tag="OQM87_003774"
     CDS             6180..>6591
                     /locus_tag="OQM87_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCS1509738.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLVSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPL"
BASE COUNT         1546 a         1715 c         1653 g         1677 t
ORIGIN      
        1 gagtaattat cctgtgcact attaatagca atgtcgccat gcacatttac cttgcagtta
       61 attgaataaa aatttaactg gcatcagtcc taaaaaaatt gatttcatcc gcaggctatt
      121 gacagaataa ttcagactgg tctttcaggc atccagacac gctaccgccc ctggcttttt
      181 agctaccaat acactgattg agcttaattt ttcaaaccct ctctgcatac aatcgttgat
      241 gagaaagggt tattgcggaa attaacttcc gaatataagg tgacattatg gtaattgaat
      301 attggctcgc caataatgca ggaggaagtg ttacagctaa cggaatagca ggcaaggtaa
      361 caattcggca attggctatt tttaagaatt atattaagtg tcattcaata tggtttttag
      421 gagtttcttt aggttgacaa tatttaatat tgcgtctcca catgcgatat ttcttaaata
      481 atgttttatt attaccgctt ttaattcagg gataatgtga ggttattacc ctcaaataat
      541 atgagataaa tagtgctgca acattgcatt tttgccccga tttatccatg atcgaattgt
      601 gacatttgtc atacaacgaa taggttttgt acttactatg gaatggattg ccgatccgtc
      661 tatctgggcc gggttaatca cgctgattgt gatcgaactg gtcctcggca ttgataacct
      721 ggtctttatt gccatcctcg ccgaaaaact accgccgaag cagcgcgacc gcgcacgggt
      781 taccgggctg ctgctggcga tgttaatgcg cctgttactg ctagcgtcaa tctcctggct
      841 ggtcaccctg actcaaccgc tgttcagctt ccgctcgttt acctttagcg cccgcgactt
      901 aatcatgctg tttggtggtt tcttcctgtt gttcaaagcc acgatggagc tgaacgaacg
      961 gctggaaggg aaagacagca ataatcccac acaacgcaaa ggtgcgaagt tctggggcgt
     1021 ggtgacgcaa attgtggtac tggacgccat tttctcactc gactcagtga ttaccgccgt
     1081 cgggatggtc gaccatttac tggtcatgat ggccgccgtg gttatcgcta tcagcctgat
     1141 gttgatggcc agcaagccgt taacgcaatt tgttaacagt cacccgacca tcgttattct
     1201 ctgcttaagc ttcctgttga tgattggctt tagcctggtg gcagaaggtt tcggcttcgt
     1261 cattccgaaa ggctacctgt acgctgccat tggtttctcg gtgatgatcg aggcactcaa
     1321 tcagttggct atctttaacc ggcgacgttt tctttccgct aatcagacgc tacgccagcg
     1381 gactaccgaa gcggtaatgc gcctgcttag cgggcaaaaa gaagatgcgg aactggacgc
     1441 cgaaaccgcg tccatgctgg tggatcatgg aaaccagcag atctttaatc cgcaggaacg
     1501 gcggatgatt gagcgggtac ttaatcttaa ccagcgtacc gtcagcagca ttatgacgtc
     1561 gcgccacgat attgagcata tcgatctcaa cgcgccggaa gaagaaatcc gccagttgct
     1621 ggagcgaaat cagcatacgc ggctggtggt taccgacggt gatgacgcag aagatttgct
     1681 cggtgttgtt cacgttatcg acctgctaca acagtcactg cgcggcgaac cgctcaacct
     1741 gcgggtgttg attcgccagc cgctggtgtt cccggaaacc ttgccgttgt taccagccct
     1801 ggagcagttc cgtaatgccc gcacgcactt tgcttttgtg gtggatgagt ttggctcggt
     1861 ggaagggatt gtgacattaa gtgacgtcac tgaaaccatt gccggtaact taccgaacga
     1921 agtggaagag atcgacgccc gccatgatat tcagaagaat gcggacggtt cctggacggc
     1981 gaatggtcat atgccgctgg aggatctggt gcaatatgtg ccgctgccgc tggatgaaaa
     2041 acgtgaatat cacaccattg ccgggctgtt gatggagtat ttgcagcgta ttccaaaacc
     2101 tggcgaagaa gttcaggtgg gggattattt gcttaaaacg ttgcaggtgg aaagccatcg
     2161 cgtgcagaag gtgcagatta taccgctgcg taaggatggc gagatggagt acgaggtgtg
     2221 atggtatcgg ccattttacc caatgctgcg taacgcctta taacttcagc aatgaagcgt
     2281 tgtctgtggt aaacggacat cacagcgggg gtgtgggtcc ggctgtaaat cgccgaatcg
     2341 tttcctgtag gctggggcag cccacatgga tgtgggctga gggcgcgttt tacagggatg
     2401 ttacctcgcg cccgtacccg atagccgaaa ggaataagat gagggaaccg cgccagcggc
     2461 gatttaccgc cgggagcccg ggttgccagg gtggtggcga ttgagccacc ctggcacgtt
     2521 caccggcttg agcgttccat agtagcgagg aacatcaagt gaacggaaca gccttcgatc
     2581 ttacatattc agaatataat agtgccgccg cgaacgcctg atgcgacgct ttacgcgtct
     2641 tatcaggcct acaggacagg tcacctggta acctacatct tctccagcaa cttcttcaca
     2701 tctttgccat tgcgggaatc tttattccgc tcggcccagt cattcaggcg acgtttcgct
     2761 tcgtcctgta gatgtttgcg cagtaactga tccacttgca ggctgtaatt gagttgctgc
     2821 cagttgccat aaacccgcag cggtactggc gtttctttga ggaaatcaat cagtttgctt
     2881 tccccgttcc agccacccac gacccgaata tcaaactggg tgtcgcaggt ttgatctgcc
     2941 agattcaaca tgccttcccc tgtcagcgcc agcacaggcg attgaccttg catgtcgttt
     3001 aacgtcacga cgccatcctt caacgtcaaa tcggtggtaa agcggtcaag ccgcgtcacg
     3061 ttatcgaagt tttcagcggc cttcacatca ccgccattac gctctaccgc ttgctgaatc
     3121 atctgctgga agttcatccc ttccatacgt gtgtcggtca tctcaacatg cgcctgccct
     3181 tgccagttgt ggcggaatgc gtcggcatct atgtcagcac cggagaaatc accagccagt
     3241 gacatttttc cggtcaacga aatcggatag ttaaacgcct tcagaatggt accaatctca
     3301 acgttctcca gccgtggctg gaagtttatc cgcggattta ttgatgtcgc gtccagcgtg
     3361 cccggcagtg aaacctttcc accgttaagc ttgccctgca gttgagtaat ttccagcaaa
     3421 cccgacttgt tggtcatttg cgtggcaaca tctgtaaaat tcattccgcg ccagcgcacg
     3481 ttactggcct gcaacaaaat atcagctgta aagccttgca gtccctgata ggccggttca
     3541 tcaatacgcg aagaaatgac cgggcgcggc aacgttgatt ggctctgccc ctgctgcgcg
     3601 gcaccgtttt caccattcgc ggtttcatta agcgggatga ggttgtcaag attcagttgc
     3661 gggaattgca gtctcagctg ccattccggt ttctctgtca gcgtgacctg tgcttgcccg
     3721 ctcagcgtac tgtcattggc ggtcaaacta atttggttaa atgaaaggcg tttatgtgac
     3781 tcctgccact gggactggaa actcccctgt ccctgaatac cttgttttgg taaatcggca
     3841 ccctgcaact gccagttaat ttgttcaata gccgctgtta aatcatgcgg ataatcagaa
     3901 gcatctaccg taccgttaag ggatatcgtg agatcgcgct gatcgcgatt aacccgcccg
     3961 gagaactcaa atgagccacg atgttgggga tcttgttcca tttgcaggcg gatattgcgg
     4021 attgtcacct gctcgtcatc ttcatgctgg aacaccagaa cgctgtccgc caccttaaga
     4081 ctggatatat caaacgacca tccgcgatcg tctgacagat ccggcaaggt attgtcgcgc
     4141 ggtgcaaccg gagcgtcttc actgcgcacc gcttccgtct gcggcgtcag ttggatcact
     4201 gcccctttta gcatcacctg cttaacgctc agttgatgac tcagtagtgg taaaagcgcc
     4261 acgtccagac gcatgttgtc ggcgcgaacc agtggctggc ttgcgccctg ggcggtgaga
     4321 gacattcgcc cggagaggat actaagctgc ggccagacgt gccaacgcag tggcccgtcg
     4381 agctgcaatt gataaccgct acgtgcagca acttgcttga ccatatagtc gcggaaatca
     4441 ttcggattca ccagcaacac taacgcagat aacccggcca ccagcacgac caggagtatc
     4501 atcagcgtcg tcagaaatcg tctcatggta tcctcaatgg gcctgaatta gtctttatcg
     4561 attcggctgg ctaccgcgcc ctgctggttg cgatatttcg catcttcacg gcggttgtaa
     4621 ggtcgcgccg ccgggccgga aagcggctca aagctcagcg caccaattaa catgcccgga
     4681 cgcagcgcca gcggcagctt accggagttg tagaactcca gcacaatgca accagaccag
     4741 cccggatcga tacggtgcgc ggtgacgtgc accatcagcc ccagacgcgc caaagaggaa
     4801 cgcccgtcca gccagcccac cagatcggct ggcagcgtca ccgactccag cgtcaccgcc
     4861 agcgccagct ctcctgggtg aagatagaac gcctcgccct cgtcgagaac gatctcatcg
     4921 ctcatcacgc ggtcaagcgc ggcgctcact tcatctttgg gaccgctcag atcgataaac
     4981 gctgccgtgt gaccacggaa ggtacgaaat ttattgccca ggcgtacatc caccgtcgcg
     5041 ccgttaatac gctccactgg cggacgtggg ttgatcgaca aacggccttc atcaagccag
     5101 gcttcaatat ctcggtcaca cagacgcatg gcattttctc ctttcgcgca tcactccctt
     5161 aacgccaatt acgtcaaggg cataccagct tatcactgaa cggtacacaa tttatcaagc
     5221 ttattcaaag aactgactta ttttcgcttt caatatatcg atcgcgatgc ggtttttccc
     5281 gccgcgcggc acgataatgt ccgcatattg tttagaaggc tcaatgaatt gcaggaacat
     5341 cgggcgcacg gttttttgat attgcgccat cacggaatcc attgaacgcc cacgctcgtt
     5401 aacgtcacgc ttgatgcggc gcatcaggca gatatccagc ggggtatcaa cgaaaatgga
     5461 gaagttaagt tcgtcacgca aacgcgcatc cgtcagcaac aaaatgcctt cgagaatgat
     5521 gaccttcttc ggctcaacct tcaccgtttc tttcatacgc gtatgttcaa catagctgta
     5581 aaccggcagg tcaattgccg agccgcgttt caacgcttgt aaatgctcaa gcaacaggct
     5641 gtgatccatc gcgctgggat ggtcgtagtt ggtcttaacg cgttcttcca tcgacagatg
     5701 gctttgatct ttgtaatagc agtcttcggg aattacgccg atgtgttcat caccgacttg
     5761 ctcacgtaat tcacgataaa gggtactggc aataagactc ttgccggaag ccgatgcgcc
     5821 agcgataccg ataatgacgc actgatgaga ctgatcagtc atatatttag cgacctgatt
     5881 aacctggatg ttaggaaggg ggcgacgaag cgccaaacgc ggcaattata gggatttcat
     5941 ccgtctgata ccagtcgaat agcgttgccg cgcgctcaga gttaattgtt gacaaagaat
     6001 tcccggtggc aaattacgtt gatcagtttt atgtaaggta aaaaatgtta tacgcagttg
     6061 cgcaaattat ccgcctttac gtcactttat gagcaattcg catataaaat gtaaaacttt
     6121 tgtactagca taaacacaga aacgaatact ggcgacctgg ccttgcggat aaagcggtaa
     6181 taagcaaaca atcacagcat gtattaattg ccctgcccca cccgctgctt cacctggtca
     6241 gtttaggttt agtctcgttt atctttactc ttgtctcgct tgagctttcg cagtttggca
     6301 cccaactcgc cccactgtgg ttcccgacgt ccatcatgat ggtggcgttt tatcgccatg
     6361 ccgggcgcat gtggccggga attgcgctga gctgttcgct gggaaatatc gccgcatcca
     6421 tcctgctttt ttccaccagc tcgctgaaca tgacctggac gaccatcaat attgttgaag
     6481 ccgtggtcgg ggcagtgctg ctacgtaaat tgctgccgtg gtataacccc ttgcaaaatc
     6541 tggctgactg gctgcgtctg gcattcggca gcgccattgt tccgcctctg t
//
LOCUS       DAJVQH010000169         6474 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJVQH010000169 DAJVQH010000000
VERSION     DAJVQH010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.170"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1100..2047
                     /gene="icsP"
                     /locus_tag="OQM87_003775"
     CDS             1100..2047
                     /gene="icsP"
                     /locus_tag="OQM87_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCS1509739.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2609..2981)
                     /locus_tag="OQM87_003776"
                     /pseudo
     CDS             complement(2609..2981)
                     /locus_tag="OQM87_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3225..3344
                     /locus_tag="OQM87_003777"
                     /pseudo
     CDS             3225..3344
                     /locus_tag="OQM87_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            3594..4460
                     /gene="ospB"
                     /locus_tag="OQM87_003778"
     CDS             3594..4460
                     /gene="ospB"
                     /locus_tag="OQM87_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCS1509740.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            4789..5529
                     /gene="phoN2"
                     /locus_tag="OQM87_003779"
     CDS             4789..5529
                     /gene="phoN2"
                     /locus_tag="OQM87_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCS1509741.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(5882..>6474)
                     /gene="ospC4"
                     /locus_tag="OQM87_003780"
     CDS             complement(5882..>6474)
                     /gene="ospC4"
                     /locus_tag="OQM87_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCS1509742.1"
                     /translation="LIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRM
                     LSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGDKKADIALYLL
                     SKFNFTKQDIAEMEKMNNNIYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQK
                     ANSGDTMLDNAMKSKDSKMIDFLLKNGAVSGKRFER"
BASE COUNT         2044 a         1211 c         1237 g         1982 t
ORIGIN      
        1 ttatccaaac tgccaagcct ctttattata agtaagatct ggcactggaa tattctcaaa
       61 aggactaaca tcatcaccac gaccttcaac caaccatttt attgcattta aatcccagaa
      121 tagttcagta gtggtcaatt tgggaaacgc acaatattgt acggttacgt tatcgcaata
      181 gagtgaactc ccgccctata cggcgttttc aaggattagt ccttaatcgg acaaccacat
      241 taaagcataa taaataatta agctttaata atatactata gatacagttc tattataaaa
      301 aatactattt taggtgatgt attaaattag tagaatattt attcttttat aaaagcacca
      361 atgaattgtc caagtaaaca tgaggtaaaa tgtgtagagg ataactgagt ttggtcactt
      421 taactgtatt agtcgcagtc tgactctcga ctttaaaagg atgggaatta cctgtactga
      481 tagtgagaaa gctttccatt gaaataagtc aaataaaagt tgatggcttc agtgctaaag
      541 tgaggtgccc gctagccgta ttcactcgaa tcggaattca atgtattgct tctgccattt
      601 ccataatatt tcacgggaaa aacaaaatct gaaaaactat acttaagtgt tgtattaagt
      661 cagttgagat acaaatacat atttatcagc acacgttacg tcaaatacag gaggttgtca
      721 tgttggtact gaaaggcacg ttggctattg cagccgtgat gacaatttat tgtcttatcg
      781 ttgttcttat ggaacgcttt tctgattgat ttcatattgg cgagagaaca agaaggtgag
      841 tagaattgtc gcggaatcct gaaactatca gcctcaggta tgaacaccaa aaggtaggta
      901 gagaaatcta tggcccccgt tactttattc gcacaatgag gtattttttt atttttacaa
      961 aagttcaata aatattataa taaaagatat gttcttggta acataaacaa atatttaatt
     1021 aaaataatgc ttggcattga cgtttatctt ataaagtaag aagatcatgg acatttcaac
     1081 caaaaaagta gagttctcga tgaaatcaaa attccttgta cttgcacttt gtgtacctgc
     1141 gatctttact acacatgcta ccactaacta tccacttttc ataccggaca acatcagtac
     1201 ggatattagt ctaggatctc tgagtggcaa aacaaaagaa cgcgtttatc atcccaagga
     1261 aggggggaga aaaattagtc aactggactg gaaatacagt aatgcgacta ttgttagagg
     1321 tggcatcgat tggaagctaa ttccaaaagt gtctttcgga gtttccggtt ggactacttt
     1381 aggtaaccag aaagcaagca tggttgataa agactggaac aattccaata cccctcaggt
     1441 atggaccgac cagagctggc atcccaatac gcatctccgt gatgctaacg aattcgagct
     1501 gaatcttaaa ggttggttat taaataattt ggattatcga cttggactaa tagcaggtta
     1561 ccaagagagt cgttacagtt ttaatgcaat gggagggagt tatatttata gtgaaaacgg
     1621 ggggaacagg aataaaaaag gggcacatcc tagtggtgaa agaacaatag gttacaaaca
     1681 gctcttcaaa ataccttata ttggattaac tgctaattac cgccatgaga attttgagtt
     1741 tggagcagaa ctgaaatata gtggttgggt tctttcatct gatacagata aacactatca
     1801 gactgagaca atttttaaag atgaaataaa aaaccaaaat tactgctctg ttgctgcgaa
     1861 tattggatac tatgtcaccc ccagtgcaaa attttatata gaaggctcca gaaattacat
     1921 ttctaataaa aaaggtgata catctcttta tgagcaaagt accaatatat ctggcaccat
     1981 taaaaatagt gcaagtattg aatatattgg ttttctcact tccgcaggta taaagtatat
     2041 tttttgaatt ggttcaccga gttgattacg ttttcatggc taaaaattgc cgcccattgt
     2101 gagtcatatt cgccctgact ttccagaacc atacgaactg cccgttgacg gacttcgggg
     2161 gaaaaacgtg tatttttagt cattctgttt acctctttct cagggcgttt agtctccagg
     2221 atttccgggg cggttcaaaa aactcttcgt tgattactgt ggacttactg ttggcattac
     2281 cgaccctgag accggagaaa taagaaccgc ttagatcatc gtggctgttc tcggggtatc
     2341 aagttacaca tgggcagagg ctacccaagg tctactatta tcgcctgaaa gcgctgatgg
     2401 aacagtgcta tcagggacat gctgatgaaa atacctaaat aatcagccag cactctatct
     2461 ttccaaatcc acagcatagc aaagagagca aaagagcctg taaattcaga aattaaggaa
     2521 acgcctcttg gtgcacataa tctatcttat gttaaatgaa atctaatttc ttgattatat
     2581 gctggatcgt tcgtgggtgg ctttgcccct atatttccag acacctgtta tcattggccc
     2641 gctgccgcag atattcccgt ggcgagcgat aatccagcgc actatgcgga tgccattcgt
     2701 tataatgctc gaatgcctct gcaaggttct ttgctgccgt taacccgtca ggtttgggca
     2761 tgatactgat gtagtcacac tttattgttt tcacgaagct ctttgctatt ctgttactct
     2821 ccggactccg caccgccgtg ttcttcggtt caacaaagtc ccaacatccg ggtgaactgg
     2881 cgtgtttcat tagcccggta gcatgaacca ttatccgtca gtcactccac tggagacgac
     2941 ggaagatcgt tgccgaagcg gtgttccacc gctcccagca tgtcgccaat gggagagcat
     3001 ctacggtctg attgaactgt ttattcaaat tgtccaccgt ctgtcagtac gctctgaacg
     3061 tcggctggtt aaaacactgc tcgctgattt tcagagggtt cacggtaaaa cagcactttt
     3121 attccggatt gcggaagcgg ctctgaataa tccggatggg ctggttaaag aggttgtgta
     3181 ccatctgcat attgttgaac cagatcgcag cggtaagaga agttcatcat acttggcgca
     3241 gttacgcgat gtttctgcta gaggtgatgc cgtaaagaac ggcagaacat taccggaaca
     3301 agatagcggt ctacctgcac tggtatcaga cccggggctt ccccggatga tctccacggt
     3361 actttaggta ctgtgtctat attatgtagg taggatcaat taactttgta gttgaaattc
     3421 tttcattgaa ataatgaata aaaaagacaa accgcattaa acaatgccag aatataattg
     3481 catttttgtt ccgttttttt aaatcatagt atacaaagaa gcttgcatac tatatgaaaa
     3541 tataacaccc tttactatga tgtttataaa acaatatatg gagtcatgta ggtatgaatt
     3601 tagatggtgt tagaccatac tgtagaatag tcaataaaaa gaatgaaagc atatcagata
     3661 ttgcatttgc acatataata aaaagggtaa aaaattcatc atgtactcac ccaaaagcag
     3721 cattggtttt tttaggagag aaaggttttt gtgatagcaa tgatgttcta tctattatgg
     3781 gacaacaaat accaagagta tttaagaaca agatgttata tgattatgtt tttaaaaatg
     3841 aaaaaagtaa aaatgatttt ctaaaaatgg ctgaatcatg gctaccacag agtgaaccaa
     3901 tagtaataaa taatgatgat gacgcattga atgctgctgc ttatttttct gtaaaaaaag
     3961 cgaaaataaa aacagtaaac gatactgatt ttaaagagta taataaggtt tatattcttg
     4021 ggcacggtag tcctggttct catcaattag gccttggttc ggaacttatt gatgtacaaa
     4081 caatcatttc aagaatgaaa gactgtggta ttctaaatgt gaaagatatc cgttttactt
     4141 catgcggctc cgctgataaa gtggctccta aaaattttaa caatgcccct gctgaaagtc
     4201 tttcttgtat ccttaactct ctgccttttt ttaaggaaaa agaatctttg ctagagcaga
     4261 taaaaaaaca ccttgaaaac gatgagtcat tgagtgatgg tctaaaaata tccggctatc
     4321 atggatatgg agttcactat ggtcaagagc tttttcccta ctcacattat cgttcaactt
     4381 caattcctgc tgatccggag catacagtaa aaagaagctc tcagaaaaag acttttatta
     4441 ttaataaaga actggattag tataaaattt ttaacctata gttaattgtt tgcatttaag
     4501 atgaagttat tttatcttta gtagaaaagt gcttccctat gagggaaaat cacaaatcat
     4561 cataatacag agacaaaacg atacgaacaa atagataaaa aacatcattc ttttaccaca
     4621 ttatttttcg tgaatatgaa aaataatgtt attactttaa tataagacta ttttttgttt
     4681 ttccatcact ctgttcaaat ttttccgcat gacttgtgtt ttttgtaaca cagctcgttt
     4741 tttacagctg accaaaatca tcaattaatt atgctaagga aataaattat gaaaaccaaa
     4801 aactttcttc ttttttgtat tgctacaaat atgattttta tcccctcagc aaatgctctg
     4861 aaggcagaag gttttctcac tcaacaaact tcaccagaca gtttgtcaat acttccgccg
     4921 cctccggcag aggattcagt agtatttcag gctgacaaag ctcattatga attcggccgc
     4981 tcgctccggg atgctaatcg tgtacgtctc gctagcgaag atgcatacta cgagaatttt
     5041 ggtcttgcat tttcagatgc ttatggcatg gatatttcaa gggaaaatac cccaatctta
     5101 tatcagttgt taacacaagt actacaggat agccatgatt acgccgtgcg taacgccaaa
     5161 gaatattata aaagagttcg tccattcgtt atttataaag acgcaacctg tacacctgat
     5221 aaagatgaga aaatggctat cactggctct tatccctctg gtcatgcatc ctttggttgg
     5281 gcagtagcac tgatacttgc ggagattaat cctcaacgta aagcggaaat acttcgacgt
     5341 ggatatgagt ttggagaaag tcgggtcatc tgcggtgcgc attggcaaag cgatgtagag
     5401 gctgggcgtt taatgggagc atcggttgtt gcagtacttc ataatacacc tgaatttacc
     5461 aaaagcctta gcgaagccaa aaaagagttt gaagaattaa atactcctac caatgaactg
     5521 accccataaa gctggacagc ctgtatcagg ctatggaggg cccatagaca aatctaccct
     5581 atatgagcat aggaggagtc tatgggcaca ccacgtttta ctcctgaatt taagggatta
     5641 ctggaaaggc tgggacatat ccctccggca gaagcagaaa aagcttatta tgctgccatc
     5701 ggaaacgatg atctggcaac ctgagttcac agataaaaca ttctctagga aactcggggc
     5761 ggttccgttc accacatgca atgtggtgtt gcaggggaac ggtctgcacc atcccctata
     5821 tcgatcaata taacaaaaat gacaacttca gattcagggc acaacctaaa tatattttag
     5881 gtcacctctc aaatcgtttg cctgataccg ctccattttt taataaaaaa tcaatcattt
     5941 ttgaatcttt gcttttcatt gcattgtcaa gcatagtgtc cccactattt gctttttgaa
     6001 attttttgtt tacatcaacc aatccattat tgataaaata ttctagcact ttatagttgg
     6061 caccatcttt actaagtaag tattcaacat catacagatt acaatatata ttgttgttca
     6121 ttttctccat ctctgcgata tcttgttttg taaaattaaa tttagatagt aaataaagtg
     6181 ctatatcagc ttttttatcg cctaaagaat aagcaagggc ctgatgagcc atttttttat
     6241 cagtcacttt attgttaatc tcttcataat tagatgcttt aatcgcatcc tccaaagata
     6301 tgtctctgag ttttattttt ttgaagttat ctgtacttag cattcttggt atatgatact
     6361 caagctgaaa cacaaagttt ataattctat ccagactaac aggatcaatg tttttgttat
     6421 aacaaaactc tcggaatcct tgatctttac tctttcttat aaaatcaata agat
//
LOCUS       DAJVQH010000170         6435 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJVQH010000170 DAJVQH010000000
VERSION     DAJVQH010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.171"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..153
                     /locus_tag="OQM87_003781"
     CDS             <1..153
                     /locus_tag="OQM87_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1509743.1"
                     /translation="IQLERQHGKLAEWVREQLNKKHSNVVACALANKLARIAWAITTQ
                     QKEYQA"
     gene            complement(456..1226)
                     /locus_tag="OQM87_003782"
     CDS             complement(456..1226)
                     /locus_tag="OQM87_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509744.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1271..1705)
                     /locus_tag="OQM87_003783"
                     /pseudo
     CDS             complement(1271..1705)
                     /locus_tag="OQM87_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1717..1938)
                     /locus_tag="OQM87_003784"
     CDS             complement(1717..1938)
                     /locus_tag="OQM87_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509745.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(1939..2622)
                     /locus_tag="OQM87_003785"
     CDS             complement(1939..2622)
                     /locus_tag="OQM87_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCS1509746.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2686..2997)
                     /locus_tag="OQM87_003786"
     CDS             complement(2686..2997)
                     /locus_tag="OQM87_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509747.1"
                     /translation="MKYDGDGRATARFFSDKGCRRVPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(2994..3896)
                     /locus_tag="OQM87_003787"
     CDS             complement(2994..3896)
                     /locus_tag="OQM87_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCS1509748.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4336..5295
                     /locus_tag="OQM87_003788"
     CDS             4336..5295
                     /locus_tag="OQM87_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCS1509749.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5295..5690
                     /locus_tag="OQM87_003789"
     CDS             5295..5690
                     /locus_tag="OQM87_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCS1509750.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            5873..6253
                     /locus_tag="OQM87_003790"
     CDS             5873..6253
                     /locus_tag="OQM87_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCS1509751.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6299..>6435
                     /locus_tag="OQM87_003791"
     CDS             6299..>6435
                     /locus_tag="OQM87_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1509752.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     L"
BASE COUNT         1755 a         1679 c         1560 g         1441 t
ORIGIN      
        1 atacagcttg aacgccagca tggaaaactt gctgagtggg ttcgggagca actcaacaag
       61 aagcattcga atgtggttgc ctgcgctctg gcaaataaac tggcccgaat tgcctgggca
      121 atcactactc agcaaaaaga atatcaggcc tgatatatag atgctgacaa aactcaatta
      181 gcttattgca aacaataaga tacaagttac cgtctggttt tgcgaagatt gattattgat
      241 gacatgaacg gcaaatcgac ctgattaaaa acctaacagg ggaaaatggc tcactgaagc
      301 cgaggggatt tttaggatta tcaggtgcgg aactcatcga ggcgcgggca tcccttgccc
      361 acagagacgc cggatagatt taagcaagcc caccattaat caaaaatcag tgttgcaaaa
      421 acgggggtga ccatagattt tccccgccat cgtcatcatt tccggatttt tgccagggca
      481 tccgccatgc tgcggatttc ggcccgggct tcccggacaa cttcccgcag aacaccacgg
      541 tcaaaccacc ggcggggcgg ttcatccggt atacgcagca catcgcaggc cagcccactg
      601 gacaggatat gctcaataca atcgctgacc agatacattt ccaccgccga aaaacgtgcg
      661 ccccggttca ggatggcatc gagcaccacg ggcaggtttt catcgtcttc atcgagccag
      721 caggggtgat cgctttcctt cacgaccagg cgaaagcggc gcgtctggcg ggtcagcaac
      781 gtctgaccgc ttgtggtcag cgccgcccgg atacgccgcg catcttctgc cccccggcaa
      841 ctgacactgc atactgcatc gcgcaccgca tcaagaactg ccctgatttc gtcttcggtc
      901 agccattcac aggcatacgg gaaagacacc tgcgttccgt ccgccggaag tagcggcagc
      961 gactgccact gactgccgga cagaacaagt tccgctccgg ttccggtcag tttctgaatg
     1021 gcggaggcca ggtgcggcgg cagcacgcca tcgtggcggt taacgagtaa cgtcgccagc
     1081 cggggcgcat ccagccgcac gtaaatgctg atccagcccg gatagttcgg gttacattgg
     1141 tcaacatccg tctcttccgg ccagccctgc gccagcagca ggttacgggc gtgacgacaa
     1201 atcagagcat tcagcgtttc attcataatt catgaactcc ctgtatcgcc gcccgcaggc
     1261 ggcgataaca tcagtcgttt tcgtgtgtgt tgtttttcag caggctgcgg gcctgatcaa
     1321 ccatcgcctg tcggcgggta acgtattccg ggagggggaa accgtgatcc cgggcagccc
     1381 attcacgttt ccacagtgcc tgttcagcga tctgagtcag ggtctccagc agttcagcag
     1441 ggacggtcac ctggcggttt tcgcgcacgt tgctgataat ctccatcgcc gcggcggaag
     1501 aaataccgga ctcaatccgc tagtcttccg ggatgccctc caggtgcgcc aggacggtcc
     1561 tgatttcatg atcggacagg gaaatatcca ttccgtcagc gagggcctga atctcttccg
     1621 gggaccagat aaccagcatc agcggcgtgt ctcccgaata ctttgctgcc agcgcccggc
     1681 atagcgtttc gcatgttccg tacatactct ctcccctcaa tctttgaagc aggaatccag
     1741 ttccagaatt tcggtatatg cctccatcaa gaggcggttt gcgcgaaaca acgtctgacg
     1801 caccagacgt tccggcaggg tgtcagcatt aaagctgtac cgccgctcca tctcattcat
     1861 ggttgtacgc aggtcgcaca tggcattggc cacctccgcc acttcggcgc gaagtttttc
     1921 gtgtccggca tagttcattt acgcagcctc cggcataaac cagtcatcat tcgcggcccc
     1981 ctgctgcatg gcccgctgta cggcagccag tcgttgctgt ccggcacggt gatactgctc
     2041 aagcagctcg ataccgatat accggcgtcc ggactggagg gcggcgacgc aggttgaacc
     2101 gctgcctgca aacgggtcca gcactatcgc attcgggtgt gtgaagctct caatcagcgg
     2161 ttgcaggctg gtaacgggct tttccgtcgg gtgatggcgg ttgcccgaat atttccagcc
     2221 caacacgtcc ggcagcgggt tttgtggcag acgtggacgg ccttttgcca ggatgtaggc
     2281 gcattcgtgg cgatagccca catatgcggc cttcgatgtg tagtttttgg tgaagaccag
     2341 gtgaccaaca acgctgaatc ccgcattttt ccaggcagac ataaagcgat cgacgcggtt
     2401 ccagccatag aagctcacca tcagcgcgtc ttttttgagt acgcggtaca tttcattaca
     2461 ggccggttgc agccattcat cagttttatc accagcaatg gtacgcccct gacggtcacg
     2521 aaaaccgacg agatatggcg gatcggtgag gatgaaatca atggcgttat caggaatacg
     2581 ggccataaca tcgatgcagt taccgaggac aaaacgggac ataacagatc cttctcttac
     2641 agagggcacc cctgtggtgc ccgttccccg ctgcctgtgg gctgttcatc gcgtccggta
     2701 acgggtcagg ccgaccgtgg aataccgcag ccgtcgtctt tttgacagca aggttatgcc
     2761 gcgaaagcgg cttgaagggc gttcatcaga aaagggcgac gggctggcgt acactgtcac
     2821 cgacgccagc cgggagcggt aaaagcgccc gtcccgtgcc cgccggacgc gggacactga
     2881 ccacaggcag cgtttatgcc gcgccgcgtc agccggcgca gtaaaaagag gaacacggcg
     2941 acatcctttg tcggaaaaga aacgggcggt tgcccgcccg tctccgtcat acttcatccg
     3001 ccataatgcg ccacattgtc gactatccac gcaggtccgg tgacttccgg ctgctcatcg
     3061 tcatctgtcg gggaagacca ttcaagttcc ccccacagca catcgacgag ctctccacgc
     3121 tcatacaact gccagccaca gaaatcacag ccctgttcgg cataccagtg ctccagcgtg
     3181 cagctgaaac ggcggcttaa ttctgcaata acgtcgcttt ccggctgaca ccacggcgtg
     3241 tcgaaatcca cctgaatgaa gttttctccc tgactgctga tattcaggtc atatccacaa
     3301 ggccacttca cgccataccg ttccgtatac cagtgatatg cagaaggaac accgttcaga
     3361 acaccaccat taaagccgtt cacttcaaca tccaggcggg tcggcagaac cgtcagcagg
     3421 tcaaacggca tggttttttc cggcactacg ttgtcacaca gacggttcca ccacacagat
     3481 acatcctcgt taccccatat gtcacaccag tcacctcttt ttgcggtgaa atgcacagtg
     3541 atggtttccc tggcatcatc cggtaatcct tcccagcgac gctggccagt accgctctgt
     3601 aaccagagtt catgcagcat caggcagttt tgttcatcca gcaacacacc gttctgcagg
     3661 tgtgtcagcc agcgggtgaa cgcgatattt tccgtcgata cagcaccacg tccggcagcc
     3721 gtcactccgg ggcactgttc agagcggata ttttcagtga tctgcagaag tccggcactt
     3781 ccggccagaa acagctgaat accttcattt gtggcgcggc gataaagcgg tgtgactgca
     3841 ccgctggcca gtcgtttaat ctcagcgatc tgtgccggtt caccaggaaa atacatacga
     3901 ttcgagcacc agtttggcat gggaattctc ctctcacatg cggcctccat caggccgtgc
     3961 cgcacctgta accgggcgac cgcatacgaa tgcaggtgcg gccccgaagc cgcagcgcgt
     4021 ggaggaggaa tgacgaaacg tgaccgggtg aggaggccgg cgacagcgac acacttgcat
     4081 cggatgcagc ccggttaacg tgccggcacg gcctggttaa gtagtatccg tctcagttcg
     4141 tggcgcaaaa tgagaaacca ataagaactc aaaatgaacc acaacaagaa cataaagaga
     4201 ctctaaaaga gcaacaccgc ttgacataga ctcaaaatga gtcacaataa gaaacaataa
     4261 aaaactcaaa aagatgcacc gatataaagg atgctcaatt tgagataaac acaaactcaa
     4321 aacgaggcaa aaagaatgct aaaagtatca tgcgatgatg gttcaactaa cgtaaaactg
     4381 gcctggcttg aagatggcga ggtaagaacc agtctgtcag gaaacagctt taaagaaggc
     4441 tggaacccgg gactgtttaa tgccggcaaa gtttacaatt acgtcgtgga tgaaaaaaaa
     4501 tacacctatg atttgggaag cactgctgtc ataggtacaa cacatgtcag ttatcaatac
     4561 agcaccacca atctgcttgc catacatcat gcactgctga catccggcct tcagccacag
     4621 gatgtagaac tgacagtaac attacctgtg accgaattct ttgataatga caatcaacca
     4681 aatgaagaaa gaatagaaag aaaaaaggca aatgttctcc gtgagatatc tctgaataaa
     4741 ggggaaacat ttaaaattaa aaaagttaat gtaatgcctg aatctttgcc agctgcattt
     4801 gaatcactaa aaaaagataa agtaaacaaa cttgaacgca gcctgattat cgatcttgga
     4861 ggaaccactc tggattgtgg tttaattctc ggtgcatttg aaggcatttc tgaaatccgt
     4921 ggctattcag aaatcgggac atcccgcatc actcataccg tcatgaatgc gctgacgaaa
     4981 gcctccacac catgtaatta ttttattgct gatgagttaa taaaaaacag acacgataat
     5041 gaatatctcc agacattaat aaatgatgta gctgaaataa aaaacatttc tcatgtaata
     5101 gacagagaag tgaaatctct ggcagaaagc atcaggcagg aaatttcaac attctcagga
     5161 atgaaccgaa tctatctcac tggcggagga gccgagctta tttaccctca cataaagcaa
     5221 tatttcccga atctgaaagt gaacaaggtg gatgaacctc agtttgcatt ggtgaaagca
     5281 atggtgcatg cgtaatggaa agcagtgatc caaaaaaaag aaagaaagtt gtggcatacc
     5341 tgcacccggc actttatccg caggacaacc tgacacaaca aacaatagac tctctcccag
     5401 tacaaatgcg gggagatttc tacagacaat cccttatttg cggagcagct ctttattctg
     5461 ttgacccccg actgttaacg ctaatcagcg gttttttcag tgagaaaatc actgcagaaa
     5521 atctggtgaa acttatagaa caaacaacag gatacacatc cacatcaatt gatataagtg
     5581 tgttaaaaaa catcatagag gcatcttcag aaaacaaatc agagagcatc acttcaaaag
     5641 atgatttcga agaacaaacc agacgcaatc tttctatgtt aaaaaaataa taaaagaaga
     5701 gcaggttcag tcaattacct gctcttcttt tatacatact aaaaaatcag atttttacga
     5761 atattcagat tatcataaaa aatgcaacag acaggaatgc aataattttg cattatctcg
     5821 ttgcttgtaa tgcaaatatg cattattgtc agtgtgtgac aataacagag agatgacgat
     5881 ggcgaccatt aacgcacgta ttgatgatga tataaataac caagcagatg aagtgctgaa
     5941 gctgatgaat atcagtcaaa ctcaggctat tgcagctttt tatcagtata ttactgaaca
     6001 aaagaaactg ccatttgtga tcacctcaat tgtaaaaacg ccgcatgatt tattacgtga
     6061 aagtacagac atgttagctg aagctcttgc agtgatttcc aatctacagg tgtggactga
     6121 acagcaggat gggattggaa aagcaaagct aatggagtat taccgccgcc tcgacgcact
     6181 gtactgttgt gcaaaagaga aaataggtct tctttcagat aaccgggatg ctgaactagg
     6241 ctgtgtccct taatcatctg agttatagta actgtctgtt tacacaacat attgaactat
     6301 ggctcgctac gaccttcccg atgaggcatg gaccatcatc aagcctctgt tacctcctga
     6361 acccgccaca ccacgggccg gacgcccatg ggctgagcac cgtaaaatca tcaatggcat
     6421 gttctgggtg ttatg
//
LOCUS       DAJVQH010000171         6304 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJVQH010000171 DAJVQH010000000
VERSION     DAJVQH010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.172"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            70..375
                     /locus_tag="OQM87_003792"
     CDS             70..375
                     /locus_tag="OQM87_003792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1509753.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(451..915)
                     /locus_tag="OQM87_003793"
     CDS             complement(451..915)
                     /locus_tag="OQM87_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS1509754.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(912..1787)
                     /gene="nanK"
                     /locus_tag="OQM87_003794"
     CDS             complement(912..1787)
                     /gene="nanK"
                     /locus_tag="OQM87_003794"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCS1509755.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1784..2473)
                     /locus_tag="OQM87_003795"
     CDS             complement(1784..2473)
                     /locus_tag="OQM87_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCS1509756.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2521..4011)
                     /gene="nanT"
                     /locus_tag="OQM87_003796"
     CDS             complement(2521..4011)
                     /gene="nanT"
                     /locus_tag="OQM87_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCS1509757.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4121..5014)
                     /gene="nanA"
                     /locus_tag="OQM87_003797"
     CDS             complement(4121..5014)
                     /gene="nanA"
                     /locus_tag="OQM87_003797"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCS1509758.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5136..5927)
                     /gene="nanR"
                     /locus_tag="OQM87_003798"
     CDS             complement(5136..5927)
                     /gene="nanR"
                     /locus_tag="OQM87_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCS1509759.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1605 a         1705 c         1560 g         1434 t
ORIGIN      
        1 atcagatggc tgcttaaaaa aagaacaaat ggtagtgtcc gctattgcca gtacacctca
       61 aaagttgcca tgatttacat tgaacagctt gaattaatcc ataaaagtgg tgatgtgttg
      121 tatccagtaa aaataaccag aaaatcatca ggaaaaactg ccttccacct tgttccattc
      181 ggcttaaata agactcacga ccttctagag gtggaagatg cttcagaagc aattcggctg
      241 gtgattgatg aacgtcattc cattcgctgc agtacactta cagcaactat tacgaataaa
      301 aagggcaagc gaattaagcg gacaggtata tacagtatca agggtgttaa cataaaagaa
      361 tacaacgtca ggtaatggca cagagcggtc cgtcagttca ggtttggctc tgtgccaaag
      421 atgggtcagg tcacacctga tctaataaat ttaagccatt aaatcagcct taaccttcac
      481 caccaccttt ttaatctcac ccggctcgcc aacaacgcat cccggcttat gcggctctcc
      541 tggcataaac acggcgaaca ttcccggttt caggatgatg gtttgctcgt tttcaatggc
      601 gctacaaagc tggtaatcat cttcatggtg gaactcttca cattggcgcg cagtgcctgc
      661 catgccaaac agaatccgtt cctcaccgtt taataacagc tggatatcaa tgtattgctc
      721 gtgcaattcc gcttttttct cgacgggcga ttgagtgtta aacgtcatga cattcataaa
      781 aatattgtcg ccctgtaatt cgtaacgacc cggcgctttt tcttgcggtc tggcagctaa
      841 ggccagcgtt aacgcgtcct gtaacgcagg atgtaaccca gcagacggta atgactgtac
      901 ttcacccatc atcataattt ttctccctgg gccaacagcg cagccccaag taaacctgca
      961 tcatggcggt aatgcgccgc cagtaaatca acatgaaatg ccgctggctc ctgcgccaga
     1021 tacatttcca ccaacgccag atacccttct gccagaccaa cgctgccacc aaccaccacg
     1081 cactggcaat cagttgtggc tttaatatca gcgatcagcc ttgcaagcgt acgtgcggag
     1141 cgatgaatca gttgctgcgc ctgctcatcg ccctgcccgg cgcgtgtgaa aatagttctc
     1201 gcatccgcgc cagccagctc gccctgcgct gccgccgcaa tgccgcgacc agaagcaatt
     1261 gcttccacgc aacctgtgcg tccacagccg cagactgggc cgtgtggatc ggcaagcgta
     1321 tgcccgatgt gccccgccag accgccaggg ccggtaagca gtttgccgcc gctcactaca
     1381 ccgccgccga cgccagtgga aacggtgata aagaccatat cggttatatc gccttccagc
     1441 gcctgatact ctgcccatgc tgcggcctgc gcgtcgttaa tggcaatggt cggcaaattg
     1501 gtaagttgtt ccagcgtttt gactaacgga aagtgtagca atccaccgag attatgcgga
     1561 ttcagcgcca gcaaactgcc gtcgcgaata attccggttg aagcgatggc aacccgctgc
     1621 gcatgagctt gcaacggaga gactaatgcg gataaggcat cacgcaaggc ttctggtgtc
     1681 tggctggctg gcgtaggaag ttcacgacga tcgcggatct gcccgtcagc gccaatcagc
     1741 gcggcggcaa gtttagtacc gccgatatca atcgccagta tggtcatagc accgcctttt
     1801 tcatcgctgt gttgtaccac tgacaaatgt gttcaagacg cgtaatggca gaaccgaccg
     1861 tcaccgccca cgcgccgtgg cgcatcgcat ctgccgcctg agcaggcgtg ttgtaacgcc
     1921 cttcggcaat cacccgacat ccggcttcgc tcaacgtttt caccagcgcc agatccggct
     1981 cttctggcgt ttcaggcgtg gtatagccag aaagcgtagt gccaataatt tcggctccca
     2041 gcttttggca tgccaggccg tcttccggcg ttgagcagtc ggtcatcgcc agtaaaccgt
     2101 gatgatggat acgtgccagc agcgtttcaa caggcaccgg acgcgggcgg tcggtgccgt
     2161 caatggcgat aatgtccgcg cccgcctgcg ccagcgcatc gacatcttca atataggccg
     2221 tgatgcgtac cggagaatcc tccagatcgc gtttcacaat tccaataatc ggcacgctca
     2281 ccaccgcacg cgtggcttgc agatttgcca caccttcgat gcgaatggca accgcgcccg
     2341 cctgctctgc cgctaatgcc atggctgcga cgatttcggg tttatcgagc gggctgtcag
     2401 gaaccggctg gcaggagaca atcaggccac cgttagcagc gattttttga tccagttgtg
     2461 caagtaacga catacatctt cccttagcga aaggcccggt acatagaccg ggcaacagga
     2521 ttaacttttg gttttgacta aatcgttttt ggcgctgcca aatggcacgg caccgctaaa
     2581 tggtttgccg tcgatagcgt catgagtacg caatgcttcc gggcgcagcc aacgctgaac
     2641 gcgggaaggc atatccagac caatcagcag gatcaccaca aatgtcaggc tgaacgagag
     2701 cgatgccagc gcagtaccca gatccagacg ttgagcgatc aacgcgccga tgattggggc
     2761 cagtgcgcca cccaatgcgc caacgttgta ggtaaagccc aggcccgctg cacgctggtc
     2821 ggtatcgaaa taaccgccaa tcagtttcgg taagatcccg gcgatccctt gtccaagcat
     2881 ttgctggaag aacagtaaca gaccgagcac ccagacgttt gcgccgccaa tcgcaaatac
     2941 cggaataatc agcagctgcg aggccagcag gctacaaacg tacgctttgt gggttcccag
     3001 ccagtcaccg aggaagccac ctacgcagca tcccacggcc gcgccaaagc cactaaagaa
     3061 cagcacattg gcgacagtat gcgggttata agccagatcg gttttcagat acgttggcag
     3121 cagcgcctga atcggccatg agtagaggaa agcaaacaac acgaccacca tcagcattac
     3181 gcccgttggc cagcgtttac ccgtactttg caccataaag ctgataaaga ttgcggcgca
     3241 taacagcccg agaacagcga cgattgcagc attttgcagg ttaccggcga agcagaacca
     3301 cagcgcagta gccgccgcca gtgtcattac gatattggca atgcgatgtt caccacggta
     3361 gagaatatcc accattgtgc gtactggtgc tttacctgcg tgtttctctt tccagtcttc
     3421 cgcttccggg atgtttttac gcagccagag agcaaagatg attggcaaaa tgccgataaa
     3481 gaacagcgca cgccaacccc agaccggaac caccaggcta tagacctgag cggcaacgac
     3541 ggcccccaca gagaagcctg aaatcaaaaa accactggct ttgttacgca gatgttttgg
     3601 ccagctttca atgacatagg tggcgctgga accgtattca cccgccatcc ccatgccgat
     3661 gaccagacga gcgataaaca tggtgatgta gcctggcgca aagccgcagg ccagcgtccc
     3721 ggccgagaag agaacgatgc tggtgaccat tgccagacga cgcccgtagc ggtcacccat
     3781 agcgccgagc atcaggccgc cgaaccagcg agagataaag gctgcggaga tcagacttgc
     3841 cgcctgcacc gtcgtcagcc cgaattcacc ctgtacttcg gtgagtacca gggcgattaa
     3901 aacgaaatca aaaccgtcaa gcagatatcc caaccaggca gcggaaaatg cgcgccattg
     3961 tgcacggttg agatggcgat accacgggat attctgggtt gtagtactca ttgtgagtct
     4021 cccgcggtgg gcgatgccca cacgctttgg tatgaaaatt gtagggtaca gatgcgttta
     4081 tttcccctca cccggtaggg gcgagcgagg ggaaaacaac tcacccgcgc tcttgcatca
     4141 actgctgggc cagcgccttc agttctggca gatatttttc atctaccggt ccaaacggtt
     4201 tgcggcacag cggcacagaa acgacatcca tataatgcag gacagttttc aggccgcgga
     4261 atacgcccgt tttgatcagt aaatcaatga ctttgttgca ctcagtttgc agtttctgcg
     4321 cggtctggat atcgccttct ttcagcgcct tcacgatccc ctgatatcgc cagcccatga
     4381 tgttgtaagt actgccgata ccaccatcag cgcctgccag caagccggaa gcgaagattt
     4441 cgtcgtaacc gttatagagc accagatcag gatgttcacg acggatctgt tccatctgat
     4501 agagatcgcc agaagtctgt ttcagcgcac cgacgccagg caacgtaacc agggtgttga
     4561 tctgatccag ggtcagtttt accccactca gggctggaat gttgtacacc accatcggca
     4621 aaccatccgc tgaatcaata attgcccgat agtgatcgca gtgttcttca aagctgaaag
     4681 gatagtagaa cggcgtgacg gcggagacgg catcgaagcc ataacgttta gccgatgccg
     4741 caagttgttg gctttcagcg gtgctgacgc aaccgacgtg ggcgatgagt ttaatcttac
     4801 ctttcgcctc ttcggcgacg atttccagta cctgttcacg ctcggaaagg ctttgtacaa
     4861 aggcttcgcc ggtcgaacca cccacgtata aaccgtcgat gccctgctga atattgaact
     4921 gaaccaggcg gcgcagactc gctttatcca gtgcttgttg ttgatcaaaa ggagtcagga
     4981 gtgcagccat tacgccacgt aaattcgttg ccataaatac ctctgaagtg atgcttgtct
     5041 gataaacgat atacctttat acctgttata ccagatcaat taagcaacac cccatacaga
     5101 aagcttataa tgcgatctgc ttcactaaag tggcattatt tctttttgtt ggtggtctga
     5161 ccgaaagcgt gccaggtagc agagacgctg ttgagatgcg attgcaacgc acgatcggct
     5221 tcgtcaggat catgacggcg gatcgcatca acgatcgcaa tatgctgttg ataactaacg
     5281 ttgttatgtt cgtgcagtgc ctgatcggta accgttgggc gtgcggcaat aagccagtcg
     5341 agcagggcaa cgtggatcgc catgaagatt gggttaccgg ggatctccgc cagcacgcgg
     5401 tggaaatcaa cgtctgaacg aatgaatgcc gcgttgttat ccagcgactg actgttgatt
     5461 tccagtgctt ttgccagcaa atcgatttgc tcatcggtgg cgtgttcagc cgcatagcgc
     5521 accaggctgg attcaaagaa cagacgtaat tgttcgaaat gggcaatccc accaggatga
     5581 gaaaggaaat ctttcgccat gccggaaagc tcgccgatga tggtgtccgc agaaggtcga
     5641 gagacacgag cgcgttcgcc gttgtttatt tgcaccagac ctttgcgttt taacgctgcc
     5701 agcgcttcac gcacagaagg acgcccgacg ttaaagaacg ccatcagttc gcgttcagac
     5761 ggtaattgtt caccttcgcc aaattcacga cggcggatca tctgttccag ctcttcttcc
     5821 accatttcgg agagtttttt acgcgccagc gggcggctac gcaagttgcg accaattgca
     5881 ggtgaagaat cttcggtttg cgaatcaaat gcgttcataa ggcccattct gtaaggtcag
     5941 tgtgattaac atcatcagtg acatcctatc acaggattga aagtagggga aaatggcagg
     6001 tttttctctt tgtacctcat cattaccata attaacggaa taattaacta ttgcgaaaaa
     6061 ttaatgtaac gcagataaac acatcccgat tgaattattc ataagactat tcacaagcat
     6121 tatgaatatt atgaatgtgt tcttacaaaa taatcataat cgcatatttt ttaatgaaaa
     6181 atcacctcac ctacaattaa aaacacgaca tccgcaccat aaatagactt gcaaaaaata
     6241 taacatcatt gttttcaatc tgccatttat gggattgacc gttttctttt gacacggagt
     6301 tcaa
//
LOCUS       DAJVQH010000172         6291 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJVQH010000172 DAJVQH010000000
VERSION     DAJVQH010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.173"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..2169
                     /locus_tag="OQM87_003799"
     CDS             <1..2169
                     /locus_tag="OQM87_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCS1509760.1"
                     /translation="QQGIEYARLLDVPFVFATNGDGFIFRDATAAEGECLEKQITLDD
                     FPSPAELWQKFCLWKGYTQAQLPVITQDYYDDGSGKSPRYYQLQAINKTIEAVSNGQN
                     RVLLVMATGTGKTYTAFQIIWRLWKSKNKKRILFLADRNILVDQTKNNDFQPFGTAMT
                     KVSGRTIDPAYEIHLALYQAITGPEEDQKAFKQVAPDFFDLIVIDECHRGSASEDSAW
                     REILDYFSSATQIGLTATPKETHEVSSTDYFGDPVYVYSLKEGIEDGFLAPYKVVRVD
                     IDVDLQGWRPTKGQTDLNGEVIDDRIYNQKDFDRTMVIDERTELVARTITDYLKRTNP
                     MDKTIVFCNDIDHAERMRRALVNLNPQQVKKNDKYVMKITGDDEIGKAQLDNFINPKK
                     PYPVIATTSELMTTGVDAKTCKLVVLDQNIQSMTKFKQIIGRGTRIDERYGKLWFTIL
                     DFKKATELFADERFDGIPEKVMDTTPEDIADPESDFEEKLEEISEHDDEQVTGVDEPP
                     APPYQVTDTDDVGPFPEEDEKKIRKFHVNGVAVGVIAQRVQYYDADGKLVTESFKDYT
                     RKTLLKEYASLDDFTRKWQDADRKEALIHELEQQGIIWEVLAEEVGKDLDPFDILCHV
                     VYGQPPLTRKERAENVHKRNYFTKYSEAAQAVLDNLLDKYADAGVQEIESIQVLKLKP
                     FDSMGTLPEIIKTGFGDRNGYNQALSELENEIYQLPPRSA"
     gene            2236..3696
                     /locus_tag="OQM87_003800"
     CDS             2236..3696
                     /locus_tag="OQM87_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCS1509761.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGK"
     gene            3705..5366
                     /locus_tag="OQM87_003801"
     CDS             3705..5366
                     /locus_tag="OQM87_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCS1509762.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5587..5928
                     /gene="symE"
                     /locus_tag="OQM87_003802"
     CDS             5587..5928
                     /gene="symE"
                     /locus_tag="OQM87_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCS1509763.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(5975..>6291)
                     /locus_tag="OQM87_003803"
     CDS             complement(5975..>6291)
                     /locus_tag="OQM87_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509764.1"
                     /translation="FDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRL
                     SLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESA
                     LV"
BASE COUNT         1842 a         1504 c         1445 g         1500 t
ORIGIN      
        1 caacagggca ttgaatatgc gcgcctgctg gacgttcctt ttgtttttgc caccaacggt
       61 gatggcttta tcttccgcga tgccaccgca gccgaaggtg aatgccttga aaagcaaata
      121 acgctggatg acttcccctc ccccgctgaa ctctggcaaa aattctgcct ctggaaagga
      181 tacacacaag ctcagcttcc ggtgattact caggactatt acgacgatgg cagcggcaaa
      241 tcgcctcgtt attatcaact tcaggcaatc aacaaaacca ttgaagccgt ctccaacggg
      301 caaaaccgcg ttctgctggt catggcgacc ggaacaggga aaacctatac cgcattccag
      361 atcatctggc gcctgtggaa atcaaaaaat aaaaaacgca ttttgttcct tgccgatcgc
      421 aacattctgg tcgaccaaac caaaaataat gatttccagc catttggtac ggcaatgacc
      481 aaagtcagcg gacgcaccat tgatcccgct tatgaaattc acctcgcgct ctatcaggct
      541 ataactggcc cggaggaaga ccaaaaagcg tttaaacaag tcgcaccaga tttcttcgat
      601 ctgatcgtga tcgacgaatg ccatcgcggc agcgcatccg aagacagcgc ctggcgagag
      661 atccttgatt atttcagttc cgccacccaa attggcttaa ccgccacgcc aaaagagacg
      721 catgaagtct ccagcacgga ttacttcggc gatccggttt acgtctactc actaaaagaa
      781 gggatcgaag acggcttcct cgctccttat aaagttgtcc gtgttgatat tgatgtcgac
      841 ctgcaaggct ggcgcccaac caaagggcaa actgacttaa acggcgaagt gattgacgat
      901 cgtatctata accagaaaga tttcgatcgc acgatggtga tcgacgaacg cacagaactg
      961 gttgccagaa ccattaccga ctacctcaag cgcaccaatc cgatggataa aaccatcgtc
     1021 ttctgtaacg acatcgatca tgcagaacgt atgcgccgcg ccctggttaa tctcaacccg
     1081 cagcaggtga aaaagaacga caaatacgtc atgaaaatca ccggcgatga tgaaattggc
     1141 aaagctcagt tggataactt catcaacccg aaaaaaccgt acccggttat cgcgaccact
     1201 tcagagctga tgaccactgg tgtggatgct aaaacctgca aactggtagt actagaccag
     1261 aacatccagt cgatgaccaa attcaagcaa attatcggtc gtggtacacg catcgacgaa
     1321 cgttacggca aactctggtt taccatcctc gactttaaaa aagccaccga actgtttgcc
     1381 gatgagcgtt tcgatggcat tcccgaaaaa gtcatggata ccacaccaga ggatatcgcc
     1441 gatccagaat ctgattttga agagaaactc gaagaaatca gcgaacatga cgacgaacag
     1501 gtaacaggcg ttgatgaacc gcctgcgcca ccataccagg ttaccgatac cgatgatgtc
     1561 ggcccatttc cggaagaaga cgagaagaaa atccgcaagt ttcacgtcaa cggtgtagcg
     1621 gtgggcgtta ttgcacagcg cgttcagtat tacgacgccg acggtaaact ggttaccgaa
     1681 tcctttaaag attacacccg caaaacactg ctcaaagaat atgcctcgct ggatgacttt
     1741 acccgcaagt ggcaggacgc cgatcgcaaa gaagcgctca ttcacgagct ggagcaacag
     1801 gggatcatct gggaagtact ggcagaagaa gtcggtaaag atctcgaccc gttcgacatt
     1861 ctttgccacg tagtgtatgg tcagccgccg ttaacccgca aagagcgcgc cgagaacgtg
     1921 cacaagcgga actacttcac aaaatactct gaagcagcgc aagccgtgct cgataatctg
     1981 ctggataaat acgccgatgc gggcgtacag gagatcgaaa gtattcaggt gctgaaactt
     2041 aaaccattcg acagcatggg caccttaccg gagattatta aaaccggatt tggcgaccgt
     2101 aacgggtata atcaggcgct cagcgagctg gaaaacgaaa tctaccaatt accgccccgc
     2161 tctgcttaac aactctcttt tgcgccttct gcaaccatca tgttgcagaa ggccttgttt
     2221 ttcatggaat acaacatgtc tatcagctca gtaatcaaat cattacaaga tattatgcgc
     2281 aaagatgccg gtgtggacgg cgatgcgcag cgtctcggtc agctctcctg gctgctgttt
     2341 ttgaaaatct tcgatgccca ggaagaggcg ctggaactgg agcaggataa ctatcaatat
     2401 ccgatcccac agcgttattt gtggcgcagt tgggccgcca acgcgcaggg cattaccggt
     2461 gattccctgc tggaattcgt taatgatgat ctgtttccgg cgttgaaaaa cctcactgcg
     2521 cctatcgata aaaacccacg cggctacgtg gtgaagcagg cgttcagcga tgcctataac
     2581 tatatgaaaa acggtacgct actgcgccag gtgatcaaca agctgaacga aattgacttt
     2641 accagcgcca gcgaacgcca tctgtttggt gatatttacg aacagatcct taaagatctg
     2701 caatctgcgg gcaatgcggg cgaattctat actccacgcg ccgtcactcg ctttatggtg
     2761 gatcgcgttg atccgaaact cggcgaatcc attatggacc cggcctgcgg tacgggcggt
     2821 tttcttgcct gcgcgttcga tcatgtaaag aacaaatacg tgaagagcgt cgccgatcat
     2881 cagacactgc aacaacagat ccacggtgtt gagaaaaaac agcttccgca cctgctggcg
     2941 accaccaata tgctgctgca cggcattgaa gtaccggtac aaattcgtca cgacaacacc
     3001 ctgaataaac cgctttcctc ctgggatgag caactggatg tcattgttac caacccgccg
     3061 tttggtggca cggaagaaga cggtattgag aagaactttc cggcagagat gcaaacccgc
     3121 gaaacggcgg atctgttcct gcaactgatt gtggaagtac tggcgaaaaa cggtcgtgcg
     3181 gcggtggtat tgccggatgg cacactattt ggcgaaggtg ttaaaaccaa aatcaaaaag
     3241 ctacttaccg aagagtgcaa cctgcatacc atcgtgcgtt taccgaatgg tgtgtttaat
     3301 ccctataccg gcattaaaac caacctgttg ttctttacca aaggtcagcc aaccaaagag
     3361 atttggttct atgaacatcc gtatccggcg ggcgtgaaaa actacagcaa aaccaagccg
     3421 atgaagtttg aagagtttca ggcggagatc gactggtggg gtaacgaggc cgatggtttt
     3481 gccagccgcg tcgagaatga gcaagcgtgg aaagtcagca ttgatgacgt gattgcacgc
     3541 aacttcaatc tggatattaa aaacccacat caggcggaaa ccgtcagcca tgatccggac
     3601 gaactgttag cgcagtatgc aaaacagcag gcggagatcc agacgctgcg taatcaactg
     3661 cgcgatattc ttggcgctgc gctgtctggc aagtaggtta actaatgagt gtggaaaagc
     3721 tgatcgttga tcatatggaa acctggacct cggcgttgca aacccgttcc accgccgggc
     3781 gcggcagctc cggtaagatt gatttgtatg gtattaagaa attgcgtgag ctgattctgg
     3841 aactggcggt gcgcggtaaa ctggtgccgc aggatccgaa tgatgaaccg gcgtcggacc
     3901 tgctgaagcg tattgcggcg gaaaaagcag agctggtgaa gcaaggcaaa attaaaaagc
     3961 aaaaaccact gccagaaatt agtgaggaag agaagccgtt tgaattgccg gagggatggg
     4021 agtgggtgca cctccctgat atttattgct caatttctga gtcatctaga aaaataaaat
     4081 cctccgaaat actacctgag ggaaaatatc ctgttatcga gcaaagccaa gaatttattt
     4141 ctggttattg caacaatgaa tgtctgttaa taaagttaaa taatccagtt atagtttttg
     4201 gtgatcacac aaggaatatt aaatttattg attttgattt tgttgtcggc gctgacggtg
     4261 tgaaaatttt atcacctata ttaatttgtg agcgtttctt tttttggcag ttacgatcat
     4321 ttaaattaga tgtaagagga tacgcgagac attttaaagt attaaatagc tgcttatttg
     4381 cattaccccc cattgcagaa caagagcgaa tagttgaaaa agttagctct ttgatgtccc
     4441 tctgcgacca actagaacag caatccctga ccagtctgga cgcacatcag caactggttg
     4501 aaaccctgtt gggaacgctt acagacagcc aaaacaccgc ggaactggct gaaaactggg
     4561 cgcgtattag cgagcatttc gacaccctat ttaccaccga agccagcgtg gatgcgttaa
     4621 aacagaccat tctgcaactg gccgtaatgg gcaaacttgt gccgcaggat ccgaatgacg
     4681 aacccgcctc tgaactgctc aaacgtattg cgcaggaaaa agctcaattg gtgaaagaag
     4741 ggaaaataaa aaaacaaaaa ccactgccgc caattagcga tgaggaaaaa ccgtttgaac
     4801 ttccggaagg gtgggaatgg tgtcgtattg gtaatatagt taatattaaa tctgaattag
     4861 tatcaccaaa agattattta aatttatatc aagtagcacc tgatattatt gaaaagggca
     4921 ctgggagagt gatatcaaag agaacagtga aagaatcagg agtcaaaggg ccaaatagtc
     4981 gcttttataa aggacaaatt gtttattcta aaatccgccc gtcattatcc aaagtttttt
     5041 tagcagagta taatggtctt tgcagtgctg atatgtatcc attagactgt tacattaatc
     5101 caaattactt attgaaatat atcctttcca ttccatttct tatgcaagtc aaaaaagcag
     5161 agaacagaat aaaaatgccg aaacttaatt ctgacagttt ttacaatatt attgttgcga
     5221 ttccccccta taatgaacaa caagcaatat ttgataagat aaattctatt gaagcagtat
     5281 gcaatggtct tatttcttat attgggattt atcataaaac ccaactgcat cttgcagacg
     5341 cactcactga cgcagcgata aactaattcc ctcgccctca caaaaccaat aaagcggcta
     5401 aatagccgct ttattcacat caccaaaatt tatatttcac gctaattttc atcttatttt
     5461 cattaatttt tagctggact ttccctgtat ttactgatga tatatccagg tatttagcac
     5521 ggtgcggatg tgcgccaaca cactcgcacc gctaatcaca atcactattt atagagaata
     5581 gtaatcatga ctgacacgca ttctattgca caaccgttag aagcagaagt ctccccggca
     5641 aataatcgtc aattaaccgt gagttatgcg agtcgttacc cggattacag ccgtattccc
     5701 gccatcaccc tgaaaggtca gtggctggaa gccgccggtt ttgccaccgg cacggcggta
     5761 gatgtcaaag tgatggaagg ctgtattgtc ctcaccgccc aacccgccgc cgccgaagag
     5821 agcgaactga tgcagtcact acgcaaggtg tgtaagctgt cggcgcgtaa acaaaggcag
     5881 gtacaggaat ttattggggt aattacaggt aaacagaaag tcgcctgata cattgacccg
     5941 gtagcgccag tgctaccggg tattattcgc cgcgttatac caacgcgctc tccatctgtt
     6001 tttcaaatgc ctgacgaatt aatcccataa tgtaaggaag ttgtgcgagg tcgctgaaac
     6061 caatttctac atcgccatta ccccagcgcc caacatttgt cacatctttg gcaatacctt
     6121 tcggatcgac cagttcatga aactgcatat taagcgacaa tcgcagtcgg cttttttgcg
     6181 gcacaacatc aacaaaattc gtttccgctt taaacgcaat atatagcttc aatacttcct
     6241 gcgtgatccc tgcgtccagg cgcataactt catcgcgaag atgatcgaat a
//
LOCUS       DAJVQH010000173         6173 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJVQH010000173 DAJVQH010000000
VERSION     DAJVQH010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.174"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            173..820
                     /gene="fucA"
                     /locus_tag="OQM87_003804"
     CDS             173..820
                     /gene="fucA"
                     /locus_tag="OQM87_003804"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCS1509765.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            848..1996
                     /gene="fucO"
                     /locus_tag="OQM87_003805"
     CDS             848..1996
                     /gene="fucO"
                     /locus_tag="OQM87_003805"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCS1509766.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2049..2804)
                     /gene="xni"
                     /locus_tag="OQM87_003806"
     CDS             complement(2049..2804)
                     /gene="xni"
                     /locus_tag="OQM87_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCS1509767.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTLAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2915..4282)
                     /gene="sdaB"
                     /locus_tag="OQM87_003807"
     CDS             complement(2915..4282)
                     /gene="sdaB"
                     /locus_tag="OQM87_003807"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCS1509768.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4340..5629)
                     /gene="sdaC"
                     /locus_tag="OQM87_003808"
     CDS             complement(4340..5629)
                     /gene="sdaC"
                     /locus_tag="OQM87_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCS1509769.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1572 a         1507 c         1644 g         1450 t
ORIGIN      
        1 aaagagaact tgcagttatt tactgtgatt agttgagcta ggtcacaaaa tataagcttg
       61 tgaatttctt tcttgttaat caggaagaag atggtgggta attaaacggc taattcaata
      121 gtgtgaaagg aacaacatta ttgccctgtt ttgaatcaga gagaggtagc taatggaacg
      181 aaataaactt gctcgtcaga ttattgacac ttgcctggaa atgacccgcc tgggactgaa
      241 ccaggggaca gcggggaacg tcagtgtacg ttatcaggat gggatgctga ttacgcctac
      301 aggcattcca tatgaaaaac tgacggagtc gcatattgtc tttattgatg gcaacggtaa
      361 acatgaggaa ggaaagctcc cctcaagcga atggcgtttc catatggcag cctatcaaag
      421 cagaccggat gccaacgcgg ttgttcacaa tcatgccgtt cattgcacgg cagtttccat
      481 tcttaaccga tcgatccccg ctattcacta catgattgcg gcggctggcg gtaattctat
      541 tccttgcgcg ccttatgcga cctttggaac acgcgaactt tctgaacatg ttgcgctggc
      601 tctcaaaaat cgtaaggcaa ctttgttaca acatcatggg cttatcgctt gtgaggtgaa
      661 tctggaaaaa gcgttatggc tggcgcatga agttgaagtg ctggcgcaac tttacctgac
      721 gaccctggcg attacggacc cggtgccagt gctgagcgat gaagagattg ccgtagtgct
      781 ggagaaattc aaaacctatg ggttacggat tgaagagtaa tttcgtaaag caacaaggag
      841 aaggatgatg gctaacagaa tgattctgaa cgaaacggca tggtttggtc ggggtgctgt
      901 tggggcttta accgatgagg tgaaacgccg tggttatcag aaggcgctga tcgtcaccga
      961 taaaacgctg gtgcaatgcg gcgtggtggc gaaagtgacc gataagatgg atgctgcagg
     1021 gctggcatgg gcgatttacg acggcgtagt gcccaaccca acaattactg tcgtcaaaga
     1081 agggctcggt gtattccaga atagcggcgc ggattacctg atcgctattg gtggtggttc
     1141 tccacaggat acttgtaaag caattggcat tatcagcaac aacccggagt ttgccgatgt
     1201 gcgtagcctg gaagggcttt ccccgaccaa taaacccagt gtaccgattc tggcaatccc
     1261 caccacagca ggtactgcgg cagaagtgac cattaactac gtgatcactg acgaagaaaa
     1321 acggcgcaag tttgtttgcg ttgatccgca tgatatcccg caggtggcgt ttattgatgc
     1381 tgacatgatg gatggtatgc ctccggcgct gaaagccgcg acgggtgtcg atgcgctcac
     1441 tcatgctatt gaggggtata ttacccgtgg cgcgtgggca ctaaccgatg cactgcacat
     1501 taaagcgatt gaaatcattg ctggggcgct gcgaggatcg gttgctggtg ataaggatgc
     1561 cggagaagaa atggcgctcg ggcagtatgt tgcgggtatg ggcttctcga atgttgggtt
     1621 agggttggtg catggtatgg cgcatccact gggcgcgttt tataacactc cacacggtgt
     1681 tgcgaacgcc atcctgttac cgcatgtcat gcgctataac gctgacttta ccggtgagaa
     1741 gtaccgcgat atcgcgcgcg ttatgggcgt gaaagtggaa ggtatgagcc tggaaggggc
     1801 gcgtaatgcc gctgttgaag cggtgtttgc cctcaaccgt gatgtcggta ttccgccaca
     1861 tttgcgtgat gttggtgtac gcaaggaaga catttcggca ctggctcagg cggcactgga
     1921 tgatgtttgt accggtggca acccgcgtga agcaacgctt gaggatattg tagagcttta
     1981 ccataccgcc tggtaaatgc gctgatgtga taatgccgga tacgatttgc ggcgtatccg
     2041 gctcgccgtt accgtaccaa ccgcaattgc tgtaaattgc cgtcgatatg caaatcggtt
     2101 tgtaagcggg caatatcgcg gcacagaaac gccatctctt tatgggtttc taattttttg
     2161 cgccactttt cggcaaccgc atccagattc tcatatatcc cttccagact ctgaaactcg
     2221 accagcagct gcgtggcgct ttttggtccg attcccgcaa cacccggtac ctttgaactg
     2281 ctgatccccg caagtcccca gtaatcgggc aactgctgcg gttgaacgcc aaattcttta
     2341 tcgataaatg gcgcatccag ccaacgtttc tggaagtaat cacgaatacg taatgtcggt
     2401 gaaagtaact gacagtagcc tttatctgtc gaaacaatcg ttgcctgatg cccggccagt
     2461 gtcactttga ccgccagtgt ggcggctaag tcatcggctt cgttgccgct ggctgaccag
     2521 cacgggacgc cgcgttgctc aaaggcggcg cgtaatgcag gcatctcgtc gtgcaactct
     2581 tccggcattg gcggtcgatc cgctttgtaa tctggtaaac gctgatgacg ccagccgcta
     2641 ctgcggtttt catcatcaaa aacggcgacc gcgtgggttg gctggctgtg cataatgagc
     2701 tgatcgagcg catgctggca ggtttcgaca cagggcgaac cctgaacggc atgaatgcga
     2761 cggataagat tcagtgcatc gacaataagc aaatgaacag ccacgataac ccccttaaag
     2821 atcaggcgat aagggtaaca ttcgatgccg gaacaggcta tggctggttg agaaaacagg
     2881 aagaggcttc gcaaaacgag gcctctggga gagattaatc gcaggcaacg atcttcatcg
     2941 ccaggccgcc gcgagaggtt tcgcggtact tggcgttcat gtctttacct gtttcgtaca
     3001 tggtttcgat aactttatcg aggcagacgc gcggttcgct ggtacggcgc agtgccatac
     3061 gcgcggcgtt caccgctttc actgctgcaa tggcgttacg ctcaatgcac ggtacctgaa
     3121 cctgccccgc gaccgggtca caagtcagac cgaggttgtg ctccatcgcg atttccgctg
     3181 cgatgcaaac ctgcgccggg cttgcgccta acagttctgc cagaccagct gccgccatag
     3241 agcacgccac gccaacttca ccctggcaac ccacttcggc accagaaatc gacgcgttca
     3301 tcttataaag agaaccaatg gcgctggcta ccagcaggta acgagccagt gagttagcgt
     3361 tcacttcgcg gataaactta tcgtagtacg ccagcacagc cgggataatc ccgcacgcac
     3421 cgttagtcgg cgcagtcacc acgcgaccgc cagcagcgtt ctcttcgttc actgccagtg
     3481 caaacatgtt gatccagtca acaaccgcca tcgggtcagt ggtggtttta tcctggctga
     3541 ccagcatccg gcgtagtgcc gcagcacggc gtggaacgcg cagtttgcca ggcaacacgc
     3601 cttcggtgga aataccgcgc tcaataccgc cacgcatgac ttcccagacg ttcgccaggt
     3661 gctgttccag ctcttcttta ctgtgcagcg ccagctcgtt tttcatcatc aggccagaga
     3721 gtgacagccc ggtttcctga caatgttttt gcagatcggc tgctgaactg tacggataag
     3781 gaacttcaac cggtgcgcta tcctgctggc caaaatgctc ttcatcaacg ataaagccac
     3841 cgccaataga gtagtaagtc tggctgtaaa cgactttatc gcccgccagc gcggtaatgc
     3901 gcataccgtt ttcatgcaga gaaaggttgt ctgcatggaa gttcatgcac tgatcaaccg
     3961 ggaactccac ttcatgctga ccgtttgcca gcatcaggcg accatgagta ttcacatcct
     4021 gaataaaacc ggggatggaa tcgatatcca cggtatccgg caggttaccc gccaggccca
     4081 taataatgga gatatctgtg tggtggcctt taccagtcag agagagcgag ccgtacacgt
     4141 caaccaccac gcgggtcacg tctttaagca ggttacgggc aatcagatcg tcggtaaatt
     4201 gtttacccgc tttcattggt ccaacggtat gagaactgga agggccaatg ccgattttga
     4261 aaatatcgaa tacgctaatc ataggaaata catcgcgtta aaacggagga agcgccgccc
     4321 gaaagcggcg cgaaaggact tagctgaaca gagagtagaa gattgcggag attgcaatca
     4381 gccccatcac gacaacgaat acgttgctga tgtgaccgct gtacttacgc attgccggta
     4441 ctttctgaat tgcgtacatc ggcatcagga acaggatcat cgcgatgatt gggccgccca
     4501 gggtttcaat catacccagg atgctcgggt tcagggtggc aacaatccag gtcgttacca
     4561 gcatgaacag cgcagtgata cggttcagct tgttgatttc gatagactta cctttaccac
     4621 gcagagattt aatcaccata ccgttgaagc cttcacgtgc gcccaggtag tggccgagga
     4681 aggatttggt gatagcgata atcgcgataa tcggagccat ccacgcgata accggtgcgt
     4741 taaagtggtt agccaggtaa gacagaatcg agatgttctg ctctttagcc gcagccaggt
     4801 ctgccggagt caggctcagt acgcagctga agacgaagaa cattacggtc agcaccatca
     4861 tgatgtgtgc gaatgccagg atcttcgagc atttctgttc tgccatatcg ccgtactctt
     4921 cacgcttcgc aacggcgaaa gaagagatga tcggagagtg gttgaacgag aacaccatta
     4981 ccggaattgc cagccacagg gtcatccaca gaccgtttcc ggttgcagat gcagtgtcca
     5041 gagacagcgt ttccagtgct gcgccgttcc actgcgggat caggtacaga gccagcagca
     5101 tcagtacgcc aacaaacggg aataccagaa tactcatcgc tttaacgatc atctgctcac
     5161 caaagcgaac gatggtcatc atacccacga tcaggatcag cgacagaatc gcacgcggcg
     5221 gtggcgtcat acccagctgg tgagacatga agctttcaac ggtattggtg attgccacgc
     5281 tataaaccag caggatcggg tagatagcga agaagtagag cagggtaatc agtttacctg
     5341 cgccaatacc aaagtgttct tctacaacct cggtgatgtc ttcgcccggg tttttaccag
     5401 acagtacgaa gcgagtcagg ccgcggtgag caaaaaacgt catcgggaac gcaaggatag
     5461 ccatgatgat cagcgggatc ataccgccaa caccggcgtt gattggcagg aacagcacgc
     5521 ccgcgccgat tgccgtgccg taaaggccca gcatccacat ggtgtctgtc ttgcgccagg
     5581 cactacgaga gtctttcgac gcaatcgtgc tggtttgagt cgtttccatc tatttctcct
     5641 ggaggaatgc aaataattca gcttttagcc agttcagccc gggaaggtgc ctgaccgtaa
     5701 aaatgaaatc tgtctatcgg cgatggctta atcatttacc gctgtaataa tttgagggcg
     5761 gaaagataca cacaagttat gtatctatca gtgatcttga tcccaaatac aataatctgc
     5821 ttggaagata gattatccca acgctttact ctgtaaatca aaagtaaaag agaatttaag
     5881 ggcaatattt gcccattaaa cgacatctga cttcttccgg ggcgcgcaat atatgtaggt
     5941 tgaactgaaa aaatcaatac ttttagttaa tcatggcgat aatttctttt tcatagcgat
     6001 aaaatacggt ttatgaaaat agggaaacgt ttgcgttgca acataaattg tatggcatat
     6061 taactgtgat gatatttgtc gcgtgagggt ggtaagaaac attcatataa caaatgaaat
     6121 gagttgatga gtaagtgacc aaaaacgaga aaacccggca acaaacgtca ccg
//
LOCUS       DAJVQH010000174         6151 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJVQH010000174 DAJVQH010000000
VERSION     DAJVQH010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.175"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(1..367)
                     /locus_tag="OQM87_003809"
                     /pseudo
     CDS             complement(1..367)
                     /locus_tag="OQM87_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(707..1261)
                     /locus_tag="OQM87_003810"
                     /pseudo
     CDS             complement(707..1261)
                     /locus_tag="OQM87_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1404..1526
                     /locus_tag="OQM87_003811"
                     /pseudo
     CDS             1404..1526
                     /locus_tag="OQM87_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1788..2354)
                     /gene="ipgB2"
                     /locus_tag="OQM87_003812"
     CDS             complement(1788..2354)
                     /gene="ipgB2"
                     /locus_tag="OQM87_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCS1509770.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2636..2950)
                     /locus_tag="OQM87_003813"
     CDS             complement(2636..2950)
                     /locus_tag="OQM87_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509771.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3028..3138)
                     /locus_tag="OQM87_003814"
                     /pseudo
     CDS             complement(3028..3138)
                     /locus_tag="OQM87_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3462..4139)
                     /gene="ospD1"
                     /locus_tag="OQM87_003815"
     CDS             complement(3462..4139)
                     /gene="ospD1"
                     /locus_tag="OQM87_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCS1509772.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4510..4647)
                     /locus_tag="OQM87_003816"
                     /pseudo
     CDS             complement(4510..4647)
                     /locus_tag="OQM87_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4729..5027)
                     /locus_tag="OQM87_003817"
                     /pseudo
     CDS             complement(4729..5027)
                     /locus_tag="OQM87_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5616..5775)
                     /locus_tag="OQM87_003818"
                     /pseudo
     CDS             complement(5616..5775)
                     /locus_tag="OQM87_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5779..6151)
                     /locus_tag="OQM87_003819"
                     /pseudo
     CDS             complement(5779..6151)
                     /locus_tag="OQM87_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1654 a         1359 c         1251 g         1887 t
ORIGIN      
        1 tgacagtgtt gttgcgcctt gctgccaacg aagctccact gcccatcaag ttcacagata
       61 agggcaacat cagcgtgagc gactggcgca gatgttatcc gccggggcgg gagcttttta
      121 atgtccgtat gacggtattg atgccgattt tcagcgtacg ggcagtatcg cgaactccgg
      181 caccgttgaa agccatttca gtgatttggt ttttaactcc cggtttacgg gcctcgtaac
      241 agtaagtgag ctgaaacacc cggtgacagt cacggcaacg gaatcgatca tgtccttttg
      301 ggttctgccc gtgacggtaa acctgaacgg actggcaacg ggggcaatga aagttaactg
      361 tgaccatgaa agaaccttaa aagcgggtat tataacccct aattcaacta attggaggca
      421 ttgtcatttt taatctatgg atacacttaa aaatcctctc accttacgtt aaaaaccttc
      481 tcatgtttag agttttttat tatgtttatc ataatgatat gacgatctga gtttttaaaa
      541 tcaagaagaa aagataagtt ttttaaatgc acagtgcctg tggataactt tgcgtaaatc
      601 cggaggacag atcactctga agcaagacaa cagcatctga aaacaatatg ctcacgtttc
      661 aaccacaggg cgtaagtgat cctacccacg taatatggac acaggcctaa gcgaggttct
      721 ggttttcaaa ttgttccgga ctgaggccgc cacaccaact gtgccgtcgc cagcgattgt
      781 aatcacattc gatataatta aacactgttg cccgcattat ttcccggctg ataaagtgtt
      841 ctccgtggag acattccact ttcagcgaat gaaagaagct ttccacgcag gcattatcgt
      901 agcagcagcc ctttgcgctc atactgccac gcagattatg ccacttcagc agcgcctgat
      961 aatctgctga acagtactga cttcctcggt ccgagtgaac gataacgttc cggggtctct
     1021 tacgccgcca cagcgccatc tgcagggcat cgcaggccag ttgcgccgtc aggcgtggtg
     1081 acatggacca gccaataacg gcacgtgacc acaggtcaat gaccacagcc agatacagcc
     1141 agccttcatc tgtacgtaag tacgtgatgt ctcccgccca cttctggttc ggaccactgg
     1201 cgtaaaaatc ctgctccagc agattttctg acacaggcag gccatgtgcg cggtaactga
     1261 ccgtattgct gggtaagatc aatttgctca ctggtattta ctacgtgcat cgaatgagtg
     1321 atcaagagcc gtatgacgcg agagtgtcac gtacggttcc gtaagcagcc ggagagtgaa
     1381 actctctccg gctgactcga ctcgcaggtt ccgacagcgg tgacgaacat gcagcggtgt
     1441 tgttctcgct gatcggcaca tgccgtctga acaatgtgga gccagaaaaa tggctgcgtt
     1501 acgttattga acatatccag gactgaccgg caaaccgggt acgcgatctg ttgccttgga
     1561 aagttgatct gacttctcag taaatatcaa tatgattctg acgagccgct tacctttgag
     1621 cagagaaagg ggagagggat cccacatcca tgagctagga aaatacaaaa actggctata
     1681 caacaattca atatattagg acttacttgc gaggagaaat atagcaccgc tcaggcaatt
     1741 cctgggtatt tacttttctt agtttcttta ctcgggatgt tctgtcatca gaaaggcgat
     1801 tctaaatttg taatatagtc actcatattc ccacgtaatt tctttagatc aatatattgc
     1861 acatttttct caaaaaactg atcggaaata agatttgcaa cattgcttgt tgtatcattt
     1921 ttaacgcttc tattcatccc ttcacaaagt tcgtccattt tctttttgaa ataactgctt
     1981 cctgatacaa gatgatttat ggaactttgt gcagcattga tgtctagaga aatgcgataa
     2041 tctttctcaa cctgtttaaa tatctgttta cggagattat cgccgatttt tcggctgtgt
     2101 tctgcacaaa acttatcaat acattggttc accacccggg atacgtagac ttttttttca
     2161 tcatttttcc aatctgtaat attttcaccg atttgttctg aaacagagct caaaatacta
     2221 gtatttattt tggttgatga tatttttact atccttttcc ccattgtaca gcggataatc
     2281 tcatccacct ttcctgaaaa atatcttttg tgagataaac tgattccaaa attattaaaa
     2341 gatgttccaa gcattataat cactttgtca tgtaaaatag taacattgtc attattttag
     2401 ctgattcaaa caattctgta aatgctttga gttctttact cccttggctc aatgatggac
     2461 cgtctggaat cccagagact aaactctctg agaaagagag tatgaaaaaa cttggggcag
     2521 tccctaaatg aagggaccaa cagcggtaaa ataaccggca tcattcataa tgatgccctg
     2581 ttagttgtag ttatgaagat ttacgtcatt aaaattgctg ttcgtagtgt cagtcctatg
     2641 gtctggcatc agctgaggat cgtagccgat acatcactgg cttcacgaca ttcgtgttga
     2701 ggctattcat gaaaactcca cgctgaaaac gccgttttgt ataaacggtc acggtatgcc
     2761 cggagccact gttgctgatg aattcgataa aaccttggcg tttcttgaag ctatcgttaa
     2821 tgcggataat gaaacaacga ttggtgagat tcgctctttt gccgacgccc tggacgctgt
     2881 ccggttcaat cgcaacaaaa tcaaccggca gctgagcaaa cccaaccttg cctctctggc
     2941 gctggagcat gaagtgattt ggctgggacg tagccgctga cagcttctat aaaagtatga
     3001 tcctacccac gtaatatgaa cacaggctta agtgaggttc tggttttcaa attgttcagg
     3061 actgagacct ccacaccaac tgtgccgtcg tcagcgattg taatcgcact tgatataatt
     3121 aaacactgta tatttcattt gtggagtctc cagtctgagg gggctttatg cattccgtat
     3181 tgggcactat gatgccagaa atctgcaagg ctccacacct tcctgtattt cccttcctct
     3241 atcacagtgc actttactta atagggtggg atgtgttcat tacatttatt gtgtttttta
     3301 tgattgtttt tgcgtccagt gtcgttcgtc atgggaaact ggttctatgg gtagtatcgc
     3361 tcagtctagt taactgtttg tttcttttta gaaattaagc agatcacaca attcggggag
     3421 agatccttct aagtgcaatt ctttgcagct atttagcaca cttaaaacaa ctcttgtatt
     3481 ttgtcagaaa taatattaaa catgtcttgt tcattattgt taagtctgtc tgctatttct
     3541 gtaagtttta tcccactatg atggcatata tttatatatg ccatcatagc ctcgatattc
     3601 cctttccctg cggccagaaa taatcctggt aacccctcat tatctcttac atccaatagt
     3661 ttaatcgttt cttttggtga taaatcaaaa gttttaataa aatctccata agcttgaatc
     3721 gcagcggaat gaccttcttg aagagctatg aacaaagcag attccccagc attatttcgt
     3781 gctgcaagaa cttctagttt ttcctctttg ggtatttcgg ctgccaacag cgattcaccg
     3841 tatttacgcg ctgcatcggc atttccatgc caaatcaatg aaaacaaatt atggcggtta
     3901 tactccttat tattctttgt cggaatggat atgctctgta ttttggccaa ttttctgaaa
     3961 gaaacaccac tatatccccc ccctgatttt tcttcattga tggcgtggga tatagcggta
     4021 tttgtcacgt taatgatatt atttttcatt cctttatttt cattagcagt attgctgcct
     4081 attattaggt gcatattttt gttgtttgct ggatgtaatc catagttatt tattgacatt
     4141 tttgttcatc ctttttgcta aaaaaaacaa taacctattt ttgtaatgta atagatatta
     4201 gtctattgct aatctaaaaa aaccattgcc acattgaatg gctatattca ccaataaatt
     4261 ctttcgattt cgtatattga catatcgtag atatatctta tccctaagat tcaataatcg
     4321 agtccgttta ccattctgtg caagcgcctt cactcagaag tgaccgtcaa ggcgatcacc
     4381 aaacttgata ataaagcagc tcattgccat acgccagtcc ttaaggggcg ttgtccattt
     4441 ctgtgatgct gcctagattt aactgccagc tccggcttat ctacatctaa atatgtgagc
     4501 ctaataccgc tacactttgt ccagactatg tttttctccg ggaagtgggc taaccgtctc
     4561 cataaagtga cgaacttttt tcaacagtgg ccatattgaa cggcactgat gattacgggt
     4621 tattgtctca tgaagagccg gccacatcac tgacagcagt gacttcaggc cttcagcacc
     4681 agacagcctg aaccagttaa tccatcgccc gacagattta gtaatctagg gctggttttt
     4741 cttcagctgt tgcggttcga aagtgccatt acgatcacgc agagtacgga tttccagagg
     4801 ttcatctcca gtagtgataa tcttcgttaa gcaaccattg cgggaattgc tgccagatct
     4861 aagctgattt ttctcgcacc caagatgatg agtcatatta gcattgagta cggtttcatt
     4921 ttcagcatcc aatcaaactg actgagatct tcaggatttt tgagactttt ggctagttca
     4981 ttagctaaag cccgcaactt attagatgtc aactgttttt cattcataaa ttcaccttca
     5041 tttgatatca aattaaatag ttcaacatca gagaattata caaacttatg tacaggctcc
     5101 tatgatcttc gaatcctgaa gattatagtg aaaataagtt ttcacgttga aatcccacat
     5161 agttagtata tgataaataa tcatggatga cctgattaac ctcttggggt agtgctttta
     5221 tagaagaata aatattctac taatttaata catcacctaa aatagtattg ttttttatgg
     5281 ttggttgaag gtcgtggtga tgatgttagt ccttttgaga atattccagt gccagatctt
     5341 acttataata aaggggcttg gcagtttgga taaactattg ttcttattcc actaatacaa
     5401 actttttgta aagtatatta aatatacttc atttcatact gaaatccccg gaagtattca
     5461 atcaaacaat tacaccataa aaggaagagg gggccctaat taagttgtca agcatgttta
     5521 tgatacctgc agggtataaa ttttctggct gcaagcagac acatataaaa cgtctttatt
     5581 gatcctaccc acataatatg gacacagtcg taagtgaggc tctggttttc aaattgttcc
     5641 ggactgagac caccacacca acggtgccgt cgccagcgat tgtaatcgca ctcgatataa
     5701 ctaaacactg ttgcccgcac tatttcccga tggataatag tgttctccgt ggagacattc
     5761 cattttcagc gaatgaattt tcatctgttg ctgtatccag tcatacagtg actgcatcag
     5821 tggcgcggct ctggcttttc ttgccgccag acgctgttct gctgaacatc cccggacttc
     5881 tgcctcgatg gcatacagtt caccgatacg ctgcagggct tccgtggtga tgtcggttgg
     5941 cactcttgca tgcacatcgt ggatttttct ccggacatga gccatacatg cggcttccgt
     6001 tattctgccg gattcgtaca acgcccggta accaccgtaa gcatcggcct gaagcactcc
     6061 gctgtaaccg gccagatgat tctgtggatg gatacctttc cggtccggtg agtacgcgaa
     6121 ccagaccgcc ggaggcattt ccgaaccggc g
//
LOCUS       DAJVQH010000175         6119 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJVQH010000175 DAJVQH010000000
VERSION     DAJVQH010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.176"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            616..1794
                     /gene="ltrA"
                     /locus_tag="OQM87_003820"
     CDS             616..1794
                     /gene="ltrA"
                     /locus_tag="OQM87_003820"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCS1509773.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDVTRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1877..2056
                     /locus_tag="OQM87_003821"
                     /pseudo
     CDS             1877..2056
                     /locus_tag="OQM87_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2086..2568)
                     /locus_tag="OQM87_003822"
                     /pseudo
     CDS             complement(2086..2568)
                     /locus_tag="OQM87_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2824..3804)
                     /locus_tag="OQM87_003823"
     CDS             complement(2824..3804)
                     /locus_tag="OQM87_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCS1509774.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3804..5003)
                     /locus_tag="OQM87_003824"
     CDS             complement(3804..5003)
                     /locus_tag="OQM87_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1509775.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5325..5663)
                     /locus_tag="OQM87_003825"
                     /pseudo
     CDS             complement(5325..5663)
                     /locus_tag="OQM87_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5730..6119)
                     /gene="istB"
                     /locus_tag="OQM87_003826"
                     /pseudo
     CDS             complement(5730..6119)
                     /gene="istB"
                     /locus_tag="OQM87_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
BASE COUNT         1660 a         1401 c         1460 g         1598 t
ORIGIN      
        1 gttccggtcc aggagccggg cagcggtaaa acaaggactg cccggctgtg ggtctacgtt
       61 cgtgatgacc gtaacgccgg ttcggaaatt gcagcataag ttatccatgc tctgaggtgg
      121 gtggaggtgg gccctccggt cagggtttac aggagcactg accaaacgac ccggtgctgc
      181 ttcattctaa ggaatgaggt ggtgagcgac tcgggaaaag gcagagatct actgtcacgt
      241 ggggttcggg aagctgaacg aaagtaaact gtccgatgaa gcatcgaaat cttagatgat
      301 gccaaaatgg ggccgtacag ccgtcccctg ataagcgcag aagacacctg cttactgact
      361 gcgcggcatc cggtgtgaag gcagcaggat cccgggccag accatctcag ggaacgtgag
      421 aacctgtttc cggcacgtat cgggaaacag gggcggaccc attcgtagta gtgatgaaag
      481 atctgtaatg ggtctggagc gaagggatgg ggatcacatt cattgcagaa gacacaaccc
      541 aaaaggggat gacgtaactg atgcaacgaa aatcatttga aataccaaaa gcactggtct
      601 gggcatcata tctggatgtg cgtcgcaaca agggagcccc ccggatgcga cgggcagact
      661 ttgaaaatgt tcgaccagca acgtgatggc aatctataca agatctggaa ccggctatgt
      721 tcgggaacat ggtttccgcc accagtgctg gaaaaacgga tcccgaagtc gaatggcaag
      781 gaacgcattc tggggatccc aacagtatcc gatcgaattg ctcagggagc gataaaactt
      841 ttcatggaag aaaagcttga cccgattttc catgctgatt catatggtta ccgaccaggc
      901 aaatcagctc atgacgctct gaaacaatgt gccatccggt gctggcgtta tagctggata
      961 ctggaagtgg acatcagtgc gtttttcgac catgtgagac atgatctggt gctcaaagct
     1021 ctggaacacc acgggatgcc taaatgggtc atcctgtact gtcgacgctg gatggaagcg
     1081 ccaatgcaga gttgtgaaaa tggagaatta ataacccgaa cacgaggcac tccgcagggc
     1141 ggggtcatta gtccgctact tgctaacttg ttccttcact atgcatttga tttgtggatg
     1201 gaaagagaat atcggggggt accgtttgag aggtacgctg acgatattgt agtacattgt
     1261 tcacgaatga gtgatgtaac aaggctaaag aacaggttat cggagcgctt ctcggaagtc
     1321 gggctggtcc tgaatgcagg gaaaacgaat atcgcctaca ttgacacgtt taaaaggaga
     1381 aacgtcgcaa cgagtttcac ctttctcgga tatgacttca aagtgcgtac gctgaagaat
     1441 ttcaaaggcg aacggtaccg aaaatgcatg ccgggtgcgt caaatgcagc aatgcgcaaa
     1501 ataacagaaa caatcaagaa gtggcgtata catcgctcaa cagctgagag tttgctggat
     1561 tttgcgaggc gctacaatgc gatagtgaga ggctggatcg agtactacgg aaagttctgg
     1621 tccagaaatt tcaactatcg actgtggagt gcaatgcagt cacgtctgct caagtggatg
     1681 cagtctaaat acagactttc gaaccggaag gctcagcgaa agctgacgct ggtaaggaag
     1741 gagtatccga agctatttgt tcactggtat ttactacgtg catcaaatga gtgatcaaga
     1801 gccgtatgac gcgagagtgt cacgtacggt tccgtgagca gccggggagt gaaattctct
     1861 ccggctgact cgactcgcgg gttccgacag cgatgacgaa catgcggcgg tgttgtactc
     1921 gctgatcggc acatgccgtc tgaacaatgt ggaaccagaa aaatggctgc gttacgtcat
     1981 tgagcatatc caggactggc cggcaaaccg ggtacgtgat ctgttgccct ggaaagttga
     2041 tctgagctct cagtaaatat caatacggtt ctgacgagcc gcttactttt ggcgtaattc
     2101 tgctgcgcct ttactgaaca caatgccgta ttcagcaaga agtccacgaa tgtgatttat
     2161 cagttgtgtc ctttccattg aaaccaaccg acgcatattc aaaaaagtct ggacgtcctg
     2221 ttgctccaga gtttttattg gtacgggtcg tatagttcca tgttggcagg cctcagctat
     2281 tgcctgtgcg tcgttataat catttttctg gcaccgctga taagctttaa catgttgtgc
     2341 cggaagcaat attacatcat ggtcgagttt cctgatttcc cgggcccagt aatgagatgc
     2401 cccgccagcc tccatcgcaa cagtcatttt aggctgttgc acctgccagt taagcagttg
     2461 attgcgagaa agtttggtct ttcctgcggg tttgccatga gcattgatac tgaaaagata
     2521 aaagttcgtt ttagccagat caatgccggt gattttctgt tcagacatgg tatcccgctt
     2581 acttttgtgg ttaaagttta tccatcatgg cgcaattgaa ggctgccgtg aaagaggggg
     2641 aagactatct catcggttac tgttaaacgg ctaagagaaa ctatcttttt caagcaaaga
     2701 agatacggtg aaacaggcga tttcaccgtg aaatactgtt caaaaaaaac gctaataaaa
     2761 gcaaaatttc ttttaaaaca gccaattaaa aactggtgag ttgctggcga aaaacttgaa
     2821 ttttcacagc gaattcagat tatcttttag tacaagagca atcatacgat ctaacttatc
     2881 ctgaacttcc aacggtaatc tgccgaattc atatgagaac gtacgtccct ttactcgttt
     2941 tcgagcaaat ttatctttac tatcaaattt ccataacaaa gttacaaccg ctttatcctt
     3001 tactcctgaa tcaagcaatg aagacatttc cttggttata agccgcataa tactgttttt
     3061 aacctcgtct tcagaaagat tgtcatcagc agtaataagc tcaatactcg gcgagatatt
     3121 cttcacaact tcatcaatag atatatcacc cagcctcagt cgttcactta attcagccag
     3181 ttgcctgtaa tcggcgtatg tcagctcaga ctgaacagga aaaagagaca caaaatcttt
     3241 aggtacggac gctgcttgga gggcacgagt caccttagct gccgacaatc cctctctttc
     3301 cgcaatttgc ttctggttca taccggcctc tttgagacgt actaaacgca acccaatttc
     3361 acgaatatta tgttcaagtg acgtctgcaa atctttcgcc agatgctgag cttcagaaac
     3421 agtaagctca tcatccgtta caagtacacg taatccaacc ttgcatagca aagctgctgc
     3481 ccgacgacgg gaaccatcca aaatctcaat aagatcgcca gtccgaatcc caatacatgg
     3541 atagaattgt tgtaaacgta tagttctggt gatgtctttt agtgagtccg gcgtcagggc
     3601 tgtctgttcg cggccattca cctcttcaac cacaaaagtt tgagtgtcca ctttgtcatg
     3661 atcaaccttg atctcagtaa attttgcctt tcggccagta ttgagggtaa atacatgaac
     3721 tggagccgat atttcttcag tattattcag gatatttgta ttcagcgtac ggccaatagt
     3781 cggtcggtgc tttctgtttt ccatcagtag ttggccctta taaattcaat acgatcaaaa
     3841 actgctttag cgaaatcttc tgctgccata cgtgcatttt tcaaagcttc accactgcca
     3901 acataagtaa caggattcgc agatatcacg gtatcaaacg actcgccgct tctctcaaaa
     3961 ccatcaagac gtggcattga aacatcaagc atgtcaccac cgaaaatctc ttttgtcagg
     4021 ctatggcagt atttatgatc tgacttattc gccagtttcg acatgaagcc aatatttgcc
     4081 tgcaaacggc atgaacatcc atcctgttca ataatctgta caagttcagg taaacgagcc
     4141 aaatatttaa gcgtggaatg gaaatcaact tgtgctggcg gcaccggtgt aaacatgata
     4201 tctgcggcag caatggcatt tttcaaaaat gcgtccagat gcgggcctgt atcaataagg
     4261 atgaagtcga aatcatgctt cagcttatca ataatattct cacgcaatat cgcgtgtttg
     4321 ttttgtccta atagatgctc ctcgcacaga gtatcccagt ttgatgcaat aaaggcgtcg
     4381 tcaatagaag ccggcataac ataaacgcca gggataactg acggaacgat aaaatcttcc
     4441 agtaattcct cacgactcac gttctgcaac atagcctgcg gtgcggttgt atcaaccagc
     4501 cccaccgcat gcaagtagtt cagaaacata gtggctgatg actggggatc taaatcaagg
     4561 agcagaatac gtaaatcttc tgacagcagg tgaggatgag cacgcagggc gtgagctacg
     4621 gaaacactac tcacggtttt agacacaccg ccttttagac taccgatgaa tatggaatat
     4681 gcctcactat aacgatcgcg atatttagga atgccacgat gtgcatagat atcgataatg
     4741 ttctggatgg ttaatgcata ttttgtcgcg gttcctgctg ggcgtttttc aaactgatag
     4801 ccctgagcct ccatctcacc gactgcacca tccacgcttg cgcgcgttaa ttttggaagc
     4861 ttagctacag cagactttga atagacttga taaaaaacat cagcctgaaa ctcctgcttt
     4921 tgctcctgaa tctgttttgt tagggcgagc aacattttat ctgcacgctg cgcaacctta
     4981 cttaactgct caaaactagt cattccccct ccgtgctatg taggatttta acttattaca
     5041 aaaaaaactg cattagcagg gtaatgcatg caagtacata ttgtacatgc ttatattaaa
     5101 aatgcgcgca ttgctgattt attaatcatc ggaatgaata ttactgtaag atagtatgcc
     5161 tttccgggag aaagacgctg atttcccccg aataatccag ttcaccacca cactccggac
     5221 aacagctttc ttcaggctcc agatggtgta tttcgcggga aacatgttcc ggtaacggac
     5281 ggcgatgacg tgactgtcgc aacaggcggg ggtagtgctt ccaatcagta gaacatgtgc
     5341 ttttcaatga aggagccgat gactttttcg tggatttcca cggaacgcga gaagcagatt
     5401 gttttacgtg ccagccgctt gatgcgggtg cagagggtcc ggttgttacg ttcaatacgt
     5461 tgcgtgaaga ttttgccggt caggtgtttc tccttcggca cttctctggc gtagctgccc
     5521 cagtcatcgc tggtcaccat gccgatacag aacggagtca acagggctaa cagttcccga
     5581 caggtctcat cattccgtgg cccgaaagtg taagccaaca caccgccagt tttagtgttg
     5641 tacgcatacc agagccagtg ttgtgtcaac gacggatgaa aagtgctcca cttatatctc
     5701 caccaacggc ccaatattga tccaccgttt tactcaggat tagcttctgc tataaccccg
     5761 gcctttcgtt tctgtctgag tcgatagctt tctcctttga tttgaacgac atgtgagtgg
     5821 tgtaagatac ggcccagcat cgctgaggtc agtgctgcat caccggcgaa cgtttgatcc
     5881 cactgcccga acggtagatt ggatgtcagg atcattgcgc tcttttcgta acgtttagcg
     5941 atgacctgga agaacagttt tgcttcttcc tgactgaacg gcagatagcc tatttcatca
     6001 atgatgagca ggcgggggcc attactccac gctgaagcgt cgttttataa cggccctgac
     6061 gttgtgccgt agataactga agtaacagat ctgctgctgt tgtgaagcga actttgata
//
LOCUS       DAJVQH010000176         6105 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJVQH010000176 DAJVQH010000000
VERSION     DAJVQH010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.177"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..920)
                     /gene="rspA"
                     /locus_tag="OQM87_003827"
     CDS             complement(<1..920)
                     /gene="rspA"
                     /locus_tag="OQM87_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCS1509776.1"
                     /translation="MHCERKTYQAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRE
                     LSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKA
                     KAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMK
                     TTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMH
                     HRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDC
                     KQLIEEQLIDYIRTTLTHAGGITGMRRIAD"
     gene            962..1303
                     /locus_tag="OQM87_003828"
     CDS             962..1303
                     /locus_tag="OQM87_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCS1509777.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1369..1898
                     /gene="speG"
                     /locus_tag="OQM87_003829"
                     /pseudo
     CDS             1369..1898
                     /gene="speG"
                     /locus_tag="OQM87_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1901..2611)
                     /locus_tag="OQM87_003830"
     CDS             complement(1901..2611)
                     /locus_tag="OQM87_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCS1509778.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2719..3024
                     /locus_tag="OQM87_003831"
     CDS             2719..3024
                     /locus_tag="OQM87_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCS1509779.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3223..3546
                     /locus_tag="OQM87_003832"
                     /pseudo
     CDS             3223..3546
                     /locus_tag="OQM87_003832"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4102..4701
                     /locus_tag="OQM87_003833"
                     /pseudo
     CDS             4102..4701
                     /locus_tag="OQM87_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(5293..>6105)
                     /locus_tag="OQM87_003834"
     CDS             complement(5293..>6105)
                     /locus_tag="OQM87_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509780.1"
                     /translation="NIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGERPEVVTV
                     PSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMVD
                     VLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKAL
                     SRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITVV
                     LSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA"
BASE COUNT         1580 a         1553 c         1498 g         1474 t
ORIGIN      
        1 aaatcggcaa tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata
       61 taatcgatga gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca
      121 cccactgcga tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc
      181 gctggcgtcg ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc
      241 gccgcttcaa taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca
      301 aacttgttac gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac
      361 cacagctgct cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct
      421 ttcgacatgc cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg
      481 aatcccagct cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg
      541 gtatggcaat aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc
      601 atgttggcag ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac
      661 atcgtaaccg gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg
      721 atacggtgcg catcgcgacc aataagccgc ggacaaagat gatcctgcaa ataagaggcc
      781 acggaaagct cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg
      841 gtgattttta atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctggtac
      901 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
      961 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     1021 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     1081 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     1141 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     1201 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     1261 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     1321 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     1381 gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag
     1441 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     1501 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     1561 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     1621 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     1681 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     1741 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     1801 aatatcgtaa tgccattcgc atgtgtatat tccagcatca gtatctggca gagcacaaaa
     1861 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     1921 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     1981 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     2041 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     2101 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     2161 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     2221 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     2281 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     2341 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     2401 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     2461 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     2521 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     2581 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     2641 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     2701 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     2761 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     2821 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     2881 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     2941 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     3001 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     3061 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     3121 taataattct taacccaaaa tgggtagact ccctctattg ttagcgcgct aaatattcaa
     3181 tatataaaca tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     3241 atggtgggca tcagccgcag aacactcgtt aaatcaaccg cgataggttc tctggcgctg
     3301 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag
     3361 gccagcgaaa aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt
     3421 cgtctacatg ttaaagataa tgaagtgacc tgggtggaaa ctgacaatac cggcagcgat
     3481 gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     3541 aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg gtaaacgcgg tgaaggcaaa
     3601 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     3661 gtcgaacagt atggcaatga agctgtatat attcagtacc cttcggggat tgttggcggc
     3721 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     3781 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     3841 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     3901 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     3961 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     4021 acggctgccg gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg
     4081 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     4141 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     4201 tataaagcct atattcttgg tgaaggtgac gataaaacag caaaaacgcc gcagtgggct
     4261 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     4321 acaaaacccg cttatatctg ctagggctgg gggccgcaac gccaggcaaa cggcgagctg
     4381 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     4441 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     4501 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     4561 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     4621 ttcatctgga actacaagga cttgaagcgg ttactcgtta gagtcacgag agaccttagt
     4681 aggcctgagg tcctgcattg agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc
     4741 attagacggg atgtctagac ggctggtcag gcccccggtg gcctctttgt caggactcag
     4801 ggtggcaccg gggaacattg gtgtcttaat tgtattgaat gaggtctcta acttttggtc
     4861 caaaactcat cgttagctgc tcgccagctc acagccgaac ccgaactgta agcacgacca
     4921 ccagcaccag cttcagcagg aagacgacaa gatagacttt atgcaggtcc atgcgtcctc
     4981 ctgtgtctat tgtaccactg cactagataa ctagtacaaa ataaagtcca gaccgaaggc
     5041 ccagaccata gttaattaat cacattgata atccaccacc tgaagtcctg ccaacattca
     5101 gtccagcctt acccatgcta ggagtcttaa ccggggtgtc gttctgtgtc tccaccactg
     5161 gtgccgcatc aggctctaca ggaagctcca cagcaggcgc agggaccgga ggtgatacgg
     5221 atgtaccacc accagcggag aagcccttag cgatggaccg gaggccttta ctaatgctct
     5281 tacccattgc tgttacgctt cggtcttggt gaacttcagg cgtttgacta caggcaggac
     5341 tgcggttgca gcgccagtgt ccagcttaac tgtcgtagca gcttcagcga caacgaagac
     5401 cttaccgagg atttccaagt cgtgggactg agagtcgcca tcacgtgaaa ggacgaccgt
     5461 aatattctca ccgtcgtgac ggaagcagcc gaagctcagg gccgtgaagc gtttagccag
     5521 catgaacaca cctttgtcct caccgttgta atacgcgatg tcggtaccca gcatatccac
     5581 gatagcgtct tcaccagaga agccctgttt agcagccaga cgggacagcg ctttcagttg
     5641 ggactcatgg gcgatgatgc cgtaatcaga cacttcagag ccgaaagagg actcggtgtt
     5701 ctcaaacaga gcgtccatga tagcgtctgc cttagtggtc aggcttccag tcaggactgg
     5761 cagctctacg gtcttcagca gcttggagga ctccagaacg tcaaccatgc gagcgttgat
     5821 ggtacggacc acattctcac agatgttcat agcgtcctgc tggagtgtgc catgagtgaa
     5881 accagtggag gacttggcgt cccacagttt agtttgctcc gagatgagga ttgcctgttc
     5941 gataggttcg acgacaatct cctgaccaga cggaacggtc acgacttcag gtcgctcacc
     6001 accgcgtaca gggacgacca cagagcggtc agacagggtg tgggcgatga ttggggtagg
     6061 caggctattg cgggtaggca ggacttctac agggaacgga atgtt
//
LOCUS       DAJVQH010000177         6036 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJVQH010000177 DAJVQH010000000
VERSION     DAJVQH010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.178"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(127..276)
                     /locus_tag="OQM87_003835"
     CDS             complement(127..276)
                     /locus_tag="OQM87_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509781.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            1465..2280
                     /gene="sul2"
                     /locus_tag="OQM87_003836"
     CDS             1465..2280
                     /gene="sul2"
                     /locus_tag="OQM87_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1509782.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2367..2669
                     /locus_tag="OQM87_003837"
     CDS             2367..2669
                     /locus_tag="OQM87_003837"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509783.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2845..4338)
                     /locus_tag="OQM87_003838"
     CDS             complement(2845..4338)
                     /locus_tag="OQM87_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HCS1509784.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(4382..4771)
                     /locus_tag="OQM87_003839"
     CDS             complement(4382..4771)
                     /locus_tag="OQM87_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509785.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4989..5429
                     /locus_tag="OQM87_003840"
     CDS             4989..5429
                     /locus_tag="OQM87_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1509786.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1434 a         1664 c         1583 g         1355 t
ORIGIN      
        1 aaaaaaaagg aatatttcgt tttcacgagt aatacccggg cattaaaaat ccactgtaat
       61 tacatgaaaa taaagaaata aaaggtaata ccggagtatt accacgtacg cgggcaagcg
      121 aaaaagctat aacgcttccc ggccgacagg cgacccaaaa tccacagggg catgcaggtt
      181 ctcttcagta acctgcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc
      241 agtcgaatcc agttgttcat catattcaga tggcatgggg tgatatccag tagctgagag
      301 gttgttagcg ggtttcgggg cgcagccctg aaccagtcat gtagcgctag cggagtgtat
      361 actggcttac tatgttgcca cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga
      421 aaaaaggcag caccggcgcg tcagcagaat atgcgataca ggagatattc cgcttcctcg
      481 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg
      541 ggacggaaat ttcctagaag ataccaggaa gacacttagc aaagaagaga taaggccacg
      601 gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcgcaaaat ctgacgctca
      661 aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc
      721 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      781 ccgcggtttc tcattccacg cctgacactc agttccgtga aggcagttcg ctccaagctg
      841 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa
      901 cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat tagtaacagg
      961 agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg gctacactga
     1021 aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag cagttcccca
     1081 actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga
     1141 gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta tcaattaatc
     1201 ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa caggtttccc
     1261 tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct acttattgag
     1321 agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg atcaccgcaa
     1381 acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga ctcatgctcg
     1441 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
     1501 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
     1561 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
     1621 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
     1681 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
     1741 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
     1801 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
     1861 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
     1921 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
     1981 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
     2041 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
     2101 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
     2161 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
     2221 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
     2281 ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc ctttgagggc
     2341 gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca gacggtattc
     2401 gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc ggcatggcgg
     2461 ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc aaggatacgc
     2521 gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg atgggcatgg
     2581 acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc ccttttcacc
     2641 caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat gcaacccaat
     2701 ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca ctagcggctc
     2761 cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca gcattttgat
     2821 tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct cagccggtgg
     2881 cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct tcaggtgatc
     2941 aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca tggcgccgcc
     3001 gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt gcgcccatgt
     3061 catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga ccttgttgcc
     3121 cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg cgaacacccc
     3181 gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc ttgcaatgaa
     3241 atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct tgagctggta
     3301 gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg ggcggctgat
     3361 gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca cgccggcgtg
     3421 caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac tggtcggcag
     3481 agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac ggtaagtgat
     3541 cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccgagg ccagatagct
     3601 gttttcgaca tcccgttcca gcaggccttg ccgttccaga tagcgaccca cccggtgggc
     3661 gatggtgtgc gtcagctggg tgagctctgg gctggtcggc gccttgaccc agcggaaacg
     3721 cgctgagccg tgggattgct cgacatacac accgtcgaga aacagcatgt ggaagtgaac
     3781 attcagattg agcgccgatc caaaacgctg gatcagggtg accgcgcccg tcttggccac
     3841 ttggtgggta tggcccgctt tcttgaccag gtgcgtggca atgacgcggt aaacgatgcc
     3901 cagcacccac cccatgatct cgggccggct ggcaaacagg aaacgcagct gaaacgggaa
     3961 gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca gcaaggcggc
     4021 actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct tacagctgaa
     4081 agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag
     4141 ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc
     4201 ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag
     4261 ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct
     4321 ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg
     4381 cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc
     4441 cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg ttattgcgtg
     4501 caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa ttaatattct
     4561 tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat gcagtataag
     4621 atgaaccact actattatca tggccaccat taccaccacc gttaaaacta cctcccgaac
     4681 ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga ccaaaaccac
     4741 cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact tttaaaaaca
     4801 gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact acgcccgtca
     4861 aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca gcctcagacc
     4921 ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca ggagacactg
     4981 tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga aagtcgcgct
     5041 cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc
     5101 gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc acttctgaga
     5161 caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg gaaaatgctc
     5221 agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc ctaatgcttg
     5281 aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac
     5341 aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg ggattctggt
     5401 cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt accataacat
     5461 ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac cggtaaaaca
     5521 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
     5581 gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg taagtcctgt
     5641 ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg
     5701 aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg gggtgatgta
     5761 caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa aagtgatgta
     5821 aaagtgatgt atgaactgaa ggagcgatgt aattctgtag tcaggaagaa taacccgttc
     5881 atgcaggcat gaacgataca gtgaggaacg ctttttttgt cacgatctcc ccttaacctg
     5941 cgtaaatcac ccgccatggt tgattatttt tcaacctcaa aatggtcata cacctaacgc
     6001 agccgcgatt ttcatcgcaa ctgcatgaat taaaag
//
LOCUS       DAJVQH010000178         6004 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJVQH010000178 DAJVQH010000000
VERSION     DAJVQH010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.179"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..73)
                     /locus_tag="OQM87_003841"
     CDS             complement(<1..73)
                     /locus_tag="OQM87_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HCS1509787.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQ"
     gene            complement(330..611)
                     /locus_tag="OQM87_003842"
     CDS             complement(330..611)
                     /locus_tag="OQM87_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1509788.1"
                     /translation="MWGVELLAIRYAAWIKPEFEIEVYEVFKTVVRLGVGAMSRLNRI
                     DHIINTETKAISQCASQMAKWGVGGRKRLLHVARERAANEVQMYLPGMV"
     gene            complement(635..835)
                     /locus_tag="OQM87_003843"
     CDS             complement(635..835)
                     /locus_tag="OQM87_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCS1509789.1"
                     /translation="MKYPTVIVNGVSVRVDEDGRYNLNDLHAAAVANGEATEQQRPSQ
                     FLRSAQIKRFIKALWNKFNHLK"
     gene            complement(1041..1193)
                     /locus_tag="OQM87_003844"
     CDS             complement(1041..1193)
                     /locus_tag="OQM87_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509790.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSKSRLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(1181..1648)
                     /locus_tag="OQM87_003845"
     CDS             complement(1181..1648)
                     /locus_tag="OQM87_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCS1509791.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(1645..2121)
                     /locus_tag="OQM87_003846"
     CDS             complement(1645..2121)
                     /locus_tag="OQM87_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCS1509792.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
     gene            complement(2105..2428)
                     /locus_tag="OQM87_003847"
     CDS             complement(2105..2428)
                     /locus_tag="OQM87_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCS1509793.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            2653..2871
                     /locus_tag="OQM87_003848"
     CDS             2653..2871
                     /locus_tag="OQM87_003848"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509794.1"
                     /translation="MATAQFGLWRPVLISGLLQLPTAGYVATPNPAKDSCPYTSACAF
                     TTTERALANQARRLFTIIGSMLLPVVQI"
     gene            complement(3140..3763)
                     /locus_tag="OQM87_003849"
     CDS             complement(3140..3763)
                     /locus_tag="OQM87_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1509795.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTELWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(3760..3948)
                     /locus_tag="OQM87_003850"
     CDS             complement(3760..3948)
                     /locus_tag="OQM87_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCS1509796.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(3945..4307)
                     /locus_tag="OQM87_003851"
     CDS             complement(3945..4307)
                     /locus_tag="OQM87_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1509797.1"
                     /translation="MSEYQFVLPYPPSVNTYWRRRGNQYYISDKGQKYRKEVQQIILQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            complement(4304..4594)
                     /locus_tag="OQM87_003852"
     CDS             complement(4304..4594)
                     /locus_tag="OQM87_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCS1509798.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            complement(4594..5316)
                     /locus_tag="OQM87_003853"
     CDS             complement(4594..5316)
                     /locus_tag="OQM87_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCS1509799.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEDCGRINGLGLNQSFCVYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(5309..5518)
                     /locus_tag="OQM87_003854"
     CDS             complement(5309..5518)
                     /locus_tag="OQM87_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HCS1509800.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDE"
     gene            complement(5478..5879)
                     /locus_tag="OQM87_003855"
     CDS             complement(5478..5879)
                     /locus_tag="OQM87_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509801.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIQLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANTTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
BASE COUNT         1371 a         1541 c         1363 g         1729 t
ORIGIN      
        1 tctggttcat aaccagtttg taaatctcta actggaattt ctgttcttca gtcatgggaa
       61 taatctctgc cattgttggc tccgtttatc cgttaaaagg gatatcagtt aagttatccc
      121 gtgtagggta taagccatta tcgagaccac tcattgaatg gcctctgcaa taaccgatgt
      181 ctttccatca gtccgccacc acaaagaatc ttttttgcca taaggctgga ggttcatctt
      241 tcagtggctg ccagtgttat ttccccactt actggcttgg gttgtttcgc ggtactgccg
      301 taatgcaaaa actggattaa cctgcgaaat cacaccattc cgggcaaata catttgcact
      361 tcatttgccg ctctctcacg tgcaacatga agcaatcttt ttcgcccacc aacgccccac
      421 ttagccattt ggcttgcgca ctggcttatc gctttggttt cagtattgat gatgtgatcg
      481 attctattca gacgggacat tgcgccaacg ccgagacgga caaccgtttt gaaaacttca
      541 taaacttcga tttcaaattc cggcttaatc catgctgcat atctgattgc cagaagttca
      601 acaccccaca cacctggttc tgcaccacct ttgattattt taagtggttg aatttgttcc
      661 aaagtgcttt tatgaagcgt tttatctgcg cgctacgcaa aaactggctt gggcgctgtt
      721 gctctgtagc ctctccattt gcaactgctg ctgcatggag atcgtttaag ttgtagcgtc
      781 catcctcatc aacacgaacg gacacaccat tgacaataac tgttgggtac ttcatcagtg
      841 attacctttt agtgatgaac cttgtcacac aggattccgg cccacagaaa ggcaccgatt
      901 accaaaccgg catcctcaag ggtcatcctg aaaggctctg tgttcataag tcgcgcgtgt
      961 gaagcgcgtt tgttgcagat ataaaaaagc cccgcgaatg cgaggctaaa tcctggtgtt
     1021 tgtgatgact ggctcttatc tcaacgcagc cccttaccgc gcgccagatg ctcaatatca
     1081 agcatcagca atgagatgtt taatctggat ttactccaga agtgatcacc cccctgtcta
     1141 cagagccaga tgtgaaggat gatgagtaaa attatcgcta tcatcgaagg cattgcgtcc
     1201 tgatgtattc ctgaagcgtt ctcagtgctg tttggtcgcg gataattccg tcccggatac
     1261 cgagaacgtt tcgtccagca actggagaga gttcgacggt ggcatcattg cccatgccgg
     1321 aggcgctgga ggtttcggct gaggatggca cagggcattt tcctttgacg agcaccctgc
     1381 caccattatc aagcttgcgc cgaagagcat cattttcagc tttcgcatca gctaactcct
     1441 tcgtgtattt agcatcgagt gcatcagcag cacgctggcg ttgctgcatg tcagtaatgg
     1501 tggcggtcgc ctgcttcagc tcactgactt ttttatctcg ctgctctttg taggtcatgg
     1561 cgttatcacg gtaatgatta accgcccatg acaggcagac gatgatgcag ataaccagag
     1621 cggagataat cgcggtgact ctgctcatac atcaatctct ctgaccgttc cgcctgcttc
     1681 tttgaatttt gcaatcaggc tgtcagcctt atgctcgaac tgaccataac cagcgcccgg
     1741 aagtgaagcc cagatattgc tgcaacggtc aattgcctga cgaatatcac cgcgatcaat
     1801 catcggtaaa gcgccacgtt ctttaatctg ttgcagtgcc acagcgtcct ggcttttcgg
     1861 agagaagtct ttcaggccaa gctgcttacg gtaagcatcc caccaacggg aaagaagctg
     1921 ataacgtccg gcggctgttg attttagttt tgggtttagt gtgacaagtt tgcgaggatg
     1981 atcggagtaa tcagtaaata gctctccgcc tacaatgacg tcataaccat gatttctggt
     2041 tttctgacgt ccgttatcag ttccctctga ccacgccagc atatcgagga acgccttacg
     2101 ttgattattg atttccacca tcttctactc cggctttttt agcagcgaag cgtttgataa
     2161 gcgaaccaat cgagtcagta ccgatgtagc cgatgaacac gctcgttata taagcgagat
     2221 tgctacttag tccggcgaag tcgagaaggt cacgaatgaa ccaggcgatg atggcgcaca
     2281 tcgttgcgtc gattactgtt tttgtaaacg caccgccatt atatctgccg cgaaggtacg
     2341 ccattgcaaa cgcaaggatt gccccgatgc cttgttcctt tgccgcgaga atggcggcta
     2401 acaggtcatg tttttctggc atcttcatgt cttaccccca ataaggggat ttgctctatt
     2461 taattaggaa taaggtcgat tactgataga acaaatccag gctactgtgt ttagtaatca
     2521 gatttgttcg tgaccgatat gcacgggcaa aacggcatta ggttgttagc gcaacctcat
     2581 gccgcccact ttcacgaaga tcatgtgtag aaggccgcag cgtaactatc actgatgaat
     2641 tcaggatagt cagtggctac ggctcagttt ggattgtggc gaccggtgct gatctccggt
     2701 ttgctgcaac tgcctacagc tggctacgtg gccacaccga atccagcgaa agattcttgc
     2761 ccttacacat cagcctgtgc attcaccaca acggaaagag cactggctaa ccaggctcgc
     2821 cgactcttca cgattatcgg ctcaatgctc ttacctgttg tgcagatata aaaaatcccg
     2881 aaaccgttat gcaggctcta actattacct gcgaactgtt tcgggattgc attttgcaga
     2941 cctctcagcc tgcgatggtt ggagttccag acgatacgtc gaagtgacca actaggcgga
     3001 atcggtagta agcgccgcct cttttcatct cactaccaca acgagcgaat taacccatcg
     3061 ttgggtcaaa tttacccaac tttattcaat aagtcaatat catgccgtta atatgttgcc
     3121 atccgtggca atcatgctgc taacgtgtga ccgcattcaa aatattgtct gcgattgact
     3181 cttccttgtg gcattgcacc accagagcgt catacagcgg cttaacagtg cgtgaccatg
     3241 tgggttgggt aaggttgggg attagcatcg ttacagcgcg atatgcggcg cttgctggca
     3301 tccttgaata gccgacgcct ttgcatcttc cacactcttt ctcaacaact ctcccccaca
     3361 gctctgtttt ggcaatatca accgcacggc ctgtaccgtg gcaatctctg catcttgcgc
     3421 ccggcgtcgc ggcactacgg caataatccg cataagcgaa tgttgcgagc acttgcagta
     3481 cctttgcctt agtatttcct tcaagctttg caacgccacg gtatttcccc gataccttgt
     3541 gtgcaaattg catcagatag ttgatagcct tttgtttgtc gttctggctg agttcgtgct
     3601 taccacagaa tgcagccatt ccgaatccgg cttgtgattg cgccatcccc atagcagcca
     3661 tcacatcagt accggaaaga gagtcagaag ccgtggcccg tggtgagtcg ctcatcatcg
     3721 ggctttttgg cgaatgaaat ttagctacgc tttcgagtct catcgtcttc ccctcttgcc
     3781 ctgtttgacc atcaggacgc cgttaactat tacgtgacgc tcgcctttgc tgtctcggtt
     3841 gtacttgagc actgttcctc ttgcgcagga aagcatcctc gccacttcgg tctgattgcc
     3901 tcgtgtctgg ataagaagct ctggtatcgt ttgaattgtg gcgttcatac gttctccagt
     3961 tcggtgattt ttattccaag ccttccgcct ggtactttca caccacgaat tacgcgaatg
     4021 tcatcgaatt gctcgtcgtc ttccgcaaat ccggcgtgga taagggagtc gagtaaacct
     4081 ttcaggatgt tatcgaggtc gcggcggcgg gagtctggaa cgtctgcgat gactttgatg
     4141 cggagtcgtg atttggtgaa aatgtctaac ttgagttgga ggattatttg ctgaacttct
     4201 tttcggtatt tctggccttt atcgctgatg tagtattggt ttccccgtct tcgccagtag
     4261 gtgttcaccg acggcgggta tggaagcaca aactgatatt cgctcatgac ttaatcttcc
     4321 cctccttcag cagtatcgcc tgcgtcctga tcacgccttc gaggtggtaa agtctggcgt
     4381 ctttgttgtc gagattatgg gtgcgtcggt cgatttcatc gtgacacgcg ctacaagccc
     4441 atgcgccgat caggtcgtca ggcttcattc ccgttccgca aattccagcc atccggtaat
     4501 gtgccagaac tgtagtttca ggattaccat tgcatacgcc gtaaatacgt acctggcatt
     4561 ctctgccgcg tgcttctttg cgtagattag ccattaagca gcctcccctg ttactttcag
     4621 cattccatta tcgagcagct ttctggtcag ccactgttga ccacgcccgg tgatttttgt
     4681 ggtgaacgat atctgtattc cgtgatttgt gttgaccgct gtttctttca ctgtgaaata
     4741 gccgcgatcc atatattcct gcattggcac attgcgccgg gaacctgaag caataaggat
     4801 tttgtgatcg cgcatccacg caaacagttt gtttggacca attccaacaa cctttgcaaa
     4861 gtttccaatc aaaattccgc tggcctcgcc aacgcgatcg gcaaactcaa ctttaggtgc
     4921 ggcaattgcg agctggtttt ccagttgcat tttctgctca gcaagatcag cagcaaggcg
     4981 caacgcttcc ggtagcgttt tggggatatt aaccgcagct tcttcaagct ctcgccagcg
     5041 gtcaacaagg cgagcggtga attccggcga caactgggct acaacgacaa tactgtctcg
     5101 ctttccttgt tcgccttcga acacatacac acaaaaactt tgatttaagc ctaacccatt
     5161 gattcttcca caatcctcaa tttgaggaag ccggataaca ccatttttag ccagcgtttc
     5221 gatggtacgt ttcacattgt catgacgctt accaaccaac tcagcgattt caatgcttgt
     5281 cattttgatg gcattgccat ttattaactc attcatcgtc ttcttcctcg tacattgagc
     5341 tattcggatc gctcatcagt tctgcgcagc agtgctcaca cacgtgaact tccagcacat
     5401 gcagcttctg accgcaatta gcgcacgtta aagctcgctc gacgctttct ttctggtatt
     5461 gaagggattg ggatgggcta agcatggctt tcaccattaa aaagtcgctt gtaagcatcg
     5521 atgtctcgtt ttgcttcacc gagctttcgt cttaattcca tgttttctga ttcaagcttt
     5581 tccatgtctt gttggtatcg atcgcggtgt tctttccatg cttttcgata cgccttcatg
     5641 tatgtcgtat tggcctttct ctttgcctga cgaactgcgt ggtggttttt cacaaaccag
     5701 tcagggtcgt taaatgctgc tctggcgcag gtataccaat aatttgttgc ctccctgttt
     5761 agccaataaa tactgataaa tggcaactgt atcgacacca tttttcgttg agactctttc
     5821 tcgccaaaca tgtgcccttt tttgatgcta aggccaaatc caggttgaat taaaagcatt
     5881 gtcatttcct cgcacgatga accgccccgg gaatcctgga gactaaactt cctgagaagg
     5941 aggtaaacag gatgactaaa aatactcgtt tttcccccga agtccgtcaa cgggcagtcc
     6001 gtat
//
LOCUS       DAJVQH010000179         5997 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJVQH010000179 DAJVQH010000000
VERSION     DAJVQH010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.180"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..268)
                     /locus_tag="OQM87_003856"
     CDS             complement(<1..268)
                     /locus_tag="OQM87_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1509802.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATE"
     gene            805..2706
                     /gene="mtlA"
                     /locus_tag="OQM87_003857"
     CDS             805..2706
                     /gene="mtlA"
                     /locus_tag="OQM87_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCS1509803.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKRSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2801..3949
                     /gene="mtlD"
                     /locus_tag="OQM87_003858"
     CDS             2801..3949
                     /gene="mtlD"
                     /locus_tag="OQM87_003858"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1509804.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3949..4536
                     /gene="mtlR"
                     /locus_tag="OQM87_003859"
     CDS             3949..4536
                     /gene="mtlR"
                     /locus_tag="OQM87_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCS1509805.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4548..4757)
                     /locus_tag="OQM87_003860"
     CDS             complement(4548..4757)
                     /locus_tag="OQM87_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509806.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5041..5403
                     /locus_tag="OQM87_003861"
     CDS             5041..5403
                     /locus_tag="OQM87_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCS1509807.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1566 a         1454 c         1489 g         1488 t
ORIGIN      
        1 gttcagtcgc tttctcctgc gacgcaacat gtgactgcat accccagcca cgctccggtt
       61 gatagagcgt cgcccagatc cacagaaacg gccagataac atgcagagta aacaggctca
      121 cccagcccgc cgtatgaata gcgtcggcat gggggtgatt gcgctttttg gcaatcagat
      181 aagggatgtc atgtatcgcg atgattccat aaaaaatcac cagaaagacg aagatcagca
      241 ctcccaacgc gaaatagttt agaaacatat ctgcctcgga ttcacgttta tcagtgttgt
      301 tttttggctg gcagccagaa gggagtcagg ctgatatttt gacaataatc cgggttcgcg
      361 attctcgcca taacaccaaa gaataatttt tagaggtgat gagttgctta gttacataac
      421 gattgtatga cgaaggcata acatgctgta gatcacatca ggtgaacgcc gtaagaaaat
      481 atcttgtgat tcagatcaca aagattcaac aaaccatcaa aacaaaaatg tgacattact
      541 cacatttaaa tgccattttt agcgaaaatc accgccttgt ttctttttta cacaggcgtt
      601 ttgtgatgag cgtcacgtca attacctctc taccccctat atttatgtga ctgatatcac
      661 acaaaagccc gtcgactgga cagttaaccg attcagtgcc agatttcgca gtatctacaa
      721 ggtccggcta cctctgccgc cacattaaca aaaaacctcg ggcttccagc ctgcgcgaca
      781 gcaaacataa gaaggggtgt ttttatgtca tccgatatta agatcaaagt gcaaagcttt
      841 ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc
      901 atcaccgcgt tatttattcc aacagggtgg ttaccgaacg agacgctggc gaagctggtc
      961 gggccgatga tcacttatct cctaccgctg ctgatcggtt ataccggtgg taagctggta
     1021 ggcggcaaac gtggcggcgt agtcggtgcc attaccacca tgggcgttat cgtcggcgca
     1081 gacatgccga tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg gtgcattaag
     1141 cacttcgacc gctgggtaga cggtaagatc aaatccggtt ttgagatgct ggtgaataac
     1201 ttctccgcag gcatcatcgg gatgatcctc gctattctgg cattcctcgg cattggcccg
     1261 attgttgaag ccctgtccaa aatgctggct gcgggcgtta acttcatggt tgtccatgac
     1321 atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc
     1381 attaaccacg gtatcttctc gccgctgggt attcagcagt cccatgaact gggtaaatca
     1441 atcttcttcc tgattgaagc taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg
     1501 ttctttggtc gtggtagcgc taaacggtct gcgggcggtg cggcaatcat ccacttcctg
     1561 ggtggtatcc acgaaatcta cttcccgtat gtgctgatga atccgcgtct gatcctcgca
     1621 gtcatcctcg gcggtatgac tggcgtgttc acgctgacta tcctgggcgg tggtctggtt
     1681 tctccggcat ctccgggttc tatccttgct gtactggcga tgacaccaaa aggtgcttat
     1741 ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt
     1801 ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg
     1861 caggacatga aagctgagtc taaaggcaca tctccgctat ctgcgggcaa tgtgactaac
     1921 gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg ttcaagtgcg
     1981 atgggcgcag gcgtgctgcg taagaaaatt caggatgcag gtctgtcgca gatttcggtc
     2041 actaacagcg cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac
     2101 ctgaccgaac gcgctatgcg ccaggttccg caggcacagc atatttcgct gaccaacttc
     2161 ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg ttgctgccca gcgccatact
     2221 gaaaacgaag tgaaagtgaa agacagcttt gacgattcca gtgctaacct gttcaaactg
     2281 ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt
     2341 gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg
     2401 gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg
     2461 gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc
     2521 gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt
     2581 aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc
     2641 atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt
     2701 aagtaatcca atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc
     2761 ccagccctct cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg
     2821 cgcaggtaat atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact
     2881 gacgtttgcc gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca
     2941 ggttcatgtg gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag
     3001 cagcattggt gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt
     3061 tggcccggtt gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa
     3121 agaacaaggt aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac
     3181 cacgcagctg aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga
     3241 agagcacgtt ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc
     3301 aactaacgat ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac
     3361 gcagttcaaa ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc
     3421 atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg
     3481 aaaactggcc ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt
     3541 ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc
     3601 agacaaacat gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa
     3661 agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct
     3721 gatcaagcca ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg
     3781 gattgctggt gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc
     3841 actgatcgct gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa
     3901 cagcgaggtt gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag
     3961 gcgcaggaca ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa
     4021 acccaggcct ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc
     4081 tttctgatca ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta
     4141 ttccgcaaag acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt
     4201 ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc
     4261 caggaatacg aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc
     4321 aacgagtacg cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg
     4381 gcggcgttac caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa
     4441 attcagcgct atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt
     4501 tccaaaatca gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct
     4561 acctgcttcg gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg
     4621 ctttcatcgg gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc
     4681 cggaacgcct ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt
     4741 tcacctaatt tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc
     4801 caattttagt ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat
     4861 aatcatccgc tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc
     4921 gtagtttttg agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga
     4981 acaaagcacc tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac
     5041 atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgcctgga cgccatccgc
     5101 caccagcagg ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa
     5161 gagaaagcca cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg
     5221 agtaaagaag cacagaagct gatgaagatg ccgttccagc gagcaattac caaaaaagag
     5281 caggctgata tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg
     5341 accgcgctgg ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt
     5401 taagcccctc agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt
     5461 caatgcgggg actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc
     5521 gatttaccgc tggttgatat tctgaaaatg catttttacc gaaatagcca tctataaaca
     5581 ataaaaatat atagaagata tcatttgtta attaacaagc atttacgtct ctttttttcg
     5641 gatttaattg tgaaatttta atcatcatat tcacaaaatc aattatctgt aaaatttttc
     5701 ccagacatgg tttctttcat atttgacatt tattaataat catcattaga atccacgaaa
     5761 aataatatat tcatacaaac acagacatct tttgaaacga atgatacatt tttatgatga
     5821 atgcttaaaa caatatttaa tcgacacaca tcactgtcat taatgtaaag caacatagct
     5881 caccaataat acaatggaca actctaccgg cattgatcgc tggtcggagg aaataaggat
     5941 ttttacatga acttgaagaa gatttttttc agtgctgtca ctgtttcagt tctctgc
//
LOCUS       DAJVQH010000180         5977 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJVQH010000180 DAJVQH010000000
VERSION     DAJVQH010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.181"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            85..315
                     /locus_tag="OQM87_003862"
                     /pseudo
     CDS             85..315
                     /locus_tag="OQM87_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
     gene            complement(584..2089)
                     /gene="glpD"
                     /locus_tag="OQM87_003863"
     CDS             complement(584..2089)
                     /gene="glpD"
                     /locus_tag="OQM87_003863"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCS1509808.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            2279..2605
                     /gene="glpE"
                     /locus_tag="OQM87_003864"
     CDS             2279..2605
                     /gene="glpE"
                     /locus_tag="OQM87_003864"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCS1509809.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            2650..3480
                     /gene="glpG"
                     /locus_tag="OQM87_003865"
     CDS             2650..3480
                     /gene="glpG"
                     /locus_tag="OQM87_003865"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCS1509810.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            3497..4255
                     /locus_tag="OQM87_003866"
     CDS             3497..4255
                     /locus_tag="OQM87_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCS1509811.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(4237..5835)
                     /gene="rtcR"
                     /locus_tag="OQM87_003867"
     CDS             complement(4237..5835)
                     /gene="rtcR"
                     /locus_tag="OQM87_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCS1509812.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPCGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
BASE COUNT         1357 a         1557 c         1595 g         1468 t
ORIGIN      
        1 ccaaagggtc ccgttttttt cgccatcatt tcggaaaagt gtccagaagg ggcaagggga
       61 gtataatttc gccattcaga aagaatggaa agaataatgc aaaatagaaa atggattttg
      121 acctcgctgg taatgacttt tttcggcatc ccaatactga cgcaattttt tgcagcggtt
      181 attgccatgc tgggggctgg gcttgccgct attcctgagg tttgcaattt actctttacg
      241 ccaacaattt accttctgct caatattttt atgctgacgc tgggcgcatt aatgctattt
      301 ttctcggggc gagtggggaa cgtgcctgag ttttgttacg ttgggtatga tggcgtgggc
      361 tttgcggtaa ttccctgaat taaaaagtag gttagatttt tgacaaagtg cgctttgttc
      421 atgccggatg cgacgtgaac gtcttatctg gcctacaaga ccatgcaaat tcaatatatt
      481 gcataaacca cgtaggcctg ataagcgtag cgcatcaggc agttttgtgt ttgtcatcag
      541 tctcaggcca gattgaaatc tgacctgatc accttacgtt aatttacgac gccagcgaca
      601 acttctgctg cgtatattcc accagccact gactcacacg agattgttga tccgcattca
      661 gccacatgcc ttgttttgtg cgacgccaca gggcatcgtc ggtgcggcgg acccattcgt
      721 gatccaccag gtatttcagt tccgcttcgt agaactcatg accgaaatct tccccgagat
      781 cgcttaccgt tcccgcattg ccgagcagca gctcgctgtt gctgccgtaa gtgcgcgcgt
      841 agtgacgtgc cagcgattca gtcaggaacg ggtaacggcg gcgcaggcga gcggcgtaat
      901 cgtcgcggtc gccttcaatg gcaccacccg gcagcacgct ctctttcgtc cacgccgggc
      961 caataccctg ataatacggc gtcagttttt ccagcgcatg ttctgccagt tttcggtagg
     1021 tggtcagctt accgccgaat accgacagca gcggtgcttt gccgttttca tcatggatat
     1081 caagggtgta atcacgggta atggcttgcg gcgagtcgga ctcatcatca cacagcggac
     1141 gcacaccgga gtaggtccag acgatatcgt cacggcttag ctgcttttta aagtgcgcgt
     1201 tatacacttt cagcaggtaa ttgatttcac tctcttcaat cttcactgct ttcggatcac
     1261 ctttgtattc cacatcggtg gtgccgatga tggaaaactc gtccatccac gggatcacga
     1321 acacaatgcg tttatcttcg ttttgcagaa tgtaggcctg cttttgggta tgcacgcgcg
     1381 gcaccacaat atggctgcct ttgatcaggc gaatgccata aggcgaaggc agatgcatcc
     1441 cctcgtcgaa gaactgtttc acccacgggc cggtggcgtt aaccaggccg cgcgcttgcc
     1501 agctatattt tttgccggta tcgatatctt ccgcttccac aatccacagg ccgttttcgc
     1561 ggcgagcaga ggtggcgcga gtccgagtaa gcacttcgcc gcctttacgc accaccatct
     1621 gggcgttggc gagtaccaga cgggcgtcgt ctacccaaca gtcagaatat tcgaatccgc
     1681 gcttaatttc cggttttaac actgaatttg cgccaaaacg caaaccaatt gatcccggca
     1741 ggctggtgcg tttacccaga tgatcgtaca taaacagacc aatgcgaatc atccacgccg
     1801 ggcgcagatg cggacgatgt ggcaggcgaa aacgcatcgg gaaggcgata tgcggagcca
     1861 ttttcagcag cacttcacgt tcagccagcg cctcgctgac caggcggaat tcatagtgct
     1921 caaggtagcg caggccaccg tgaatgagtt ttgaactggc ggaagaggtc gcgcaagcga
     1981 gatcctgcgc ctccagcatc agcacggata aaccgcgtcc agcggcgtct gccgcgatac
     2041 cagcaccatt gatgccgcca cctatcacaa tcagatcttt ggtttccatg ctgccctcat
     2101 tcactttcgt taaagctcat aaatgttcgt tatcgaacat attagcaaag aatcgcgctt
     2161 tgggtaacat tgaaaaaaca ttttagagtg atatgtataa cattatggcg tttatctgcc
     2221 gcttcgacgt aaactgtgcg gtaaatttgc ccacttgttt gtaaagaaag agagacgcat
     2281 ggatcagttc gaatgtatta acgttgccga cgcgcaccag aagttgcagg aaaaagaggc
     2341 ggtgctggtc gatattcgcg atccacagag tttcgctatg gggcatgcgg tgcaggcttt
     2401 ccatttaacc aacgacacgc tgggcgcgtt tatgcgtgat aacgactttg acactccggt
     2461 gatggtgatg tgttatcacg gcaatagcag caaaggggcg gcgcagtatc tgctgcaaca
     2521 gggctacgat gtggtctata gcattgacgg cggctttgaa gcctggcagc gacagtttcc
     2581 cgcagaggtg gcgtacggcg cgtaacgctt tatactgtcc ccttttgtgt ggaataagcg
     2641 acagcaacga tgttgatgat tacctctttt gctaaccccc gcgtggcgca ggcgtttgtt
     2701 gattacatgg cgacgcaggg tgttatcctc acgattcaac aacataacca aagcgatgtc
     2761 tggctggcgg atgagtccca ggccgagcgc gtgcgggcgg agctggcgcg ttttctcgaa
     2821 aacccggcag atccgcgtta tctggcggcc agctggctgg caggccatac cggcagtggc
     2881 ctgcattatc gccgttatcc tttctttgct gccttgcgtg aacgcgcagg tccggtgacc
     2941 tgggtgatga tgatcgcctg cgtggtggtg tttattgcca tgcaaattct cggcgatcag
     3001 gaagtgatgt tatggctggc ctggccattc gatccaacac tgaaatttga gttctggcgt
     3061 tacttcaccc acgcgttaat gcacttctcg ctgatgcata tcctctttaa cctgctttgg
     3121 tggtggtatc tcggcggcgc ggtggaaaaa cgcctcggta gcggtaagct aattgtcatt
     3181 acgcttatca gcgccctgtt aagcggctat gtgcagcaaa aattcagcgg gccgtggttt
     3241 ggcgggcttt ctggcgtggt gtatgcgctg atgggctacg tctggctacg tggcgaacgc
     3301 gatccgcaaa gtggcattta cctgcaacgt gggttaatta tctttgcgct gatctggatt
     3361 gtcgccggat ggtttgattt gtttgggatg tcgatggcga acggagcaca catcgccgga
     3421 ttagccgtgg gtttagcgat ggcttttgtt gattcgctca atgcgcgaaa acgaaaataa
     3481 ttccagggat ttataaatga aacaaacaca acgtcacaac ggtattatcg aactggttaa
     3541 acagcagggt tatgtcagta ccgaggaact ggtggagcac ttctccgtca gcccgcagac
     3601 tattcgccgc gacctcaacg agctggcaga gcaaaacctg atcctgcgcc atcatggcgg
     3661 cgcggcgctg ccttccagtt cggttaacac gccgtggcac gatcgtaaag ccacccagac
     3721 cgaagaaaaa gagcgcatcg cccgcaaagt ggcggagcaa atccccaatg gctcgacgct
     3781 gtttatcgat atcggcacca cgccggaagc ggtggcgcac gcgctgctca atcacagcaa
     3841 tttgcgcatt gtcaccaaca atctcaacgt tgctaacacg ttgatggtaa aagaagattt
     3901 ccgcattatt ctcgccggtg gcgaattacg tagccgcgat ggcgggatca ttggcgaagc
     3961 gacgctcgat tttatctccc agttccgcct tgatttcggc attctgggga tcagcggcat
     4021 cgacagcgac ggctcgctgc tggagttcga ttaccacgaa gttcgtacca agcgcgccat
     4081 tattgagaac tcgcgccacg ttatgctggt ggttgaccat tcaaaatttg gccgtaatgc
     4141 gatggtcaat atggggagca tcagcatggt tgatgccgtc tacaccgacg ccccgccgcc
     4201 agtaagcgtg atgcaggtgc tgacggacca ccatattcaa ctggagctgt gctgatcctg
     4261 cacggcctcc cacgtcagac caaaacgcgc caggtatttg cgtagccgat ccgcgtcatt
     4321 gacgctggct ttgccctggc gcgaaacatc aaaaagctgt cgtccggcag cagaaagcga
     4381 ctttgcctgg cggcagatag cgataacgtg ttccagttgc atacggtcga agagatcgat
     4441 gttttctgcc tctgcgccca gcaacgccgt aagcgcggag gggcggctct cctgccagtt
     4501 atagcgcaga cggtttatct catcttccac cacgtccaga gtgatgcgtc cgctagtggc
     4561 aaaggtggcc atccgcgtga cgctggcaga aagttcgcga aagttaccgc gccatgtagc
     4621 ctggggagat gttgcaaagg ccagccaggc acgccgcgct tcggtgttaa aacgcacgct
     4681 gtcaccagtg agtgtggcgt ggcgctccac ttcataatcc aggttcggtt caatatcttc
     4741 ctggcgctgg cgtagacccg gcagggtgaa ggtccagaga ttgatccgcg cgtacagatc
     4801 ttcgcggaat ttgccttcgg caaccagctg gcgcaaatcg cgcaccgttc cggcgataag
     4861 ctgaaaatca ctgctcacct ggcgatcgct gccaaacggg taaaaggttt tctcttcaat
     4921 agctttcagc agcattgcct gttcgtctgc gcccagttcg ccaatctcat caagaaacaa
     4981 cattccgccg ttggcgctgc gtaataatcc ttcacgagat tcccgcgccc cggtaaacgc
     5041 gcctttcaca tgaccaaaca gcgtcgacat cgcggtatcg ccgcgcaggg tggcgcagtt
     5101 cacttccaca aacgcgccgc taaactgatg ccgtgcctgt tttaactcga agatgcgtcg
     5161 cgccagaaat gacttacctg cgccggtcgg gccgttaagc agaatcggcg cgcgggattt
     5221 aatcgccact ttttcgatct gctcaatcat gcggttgaag tgggggttac gcgtggcaat
     5281 accggatttg aggaaatcga gcgtttgctg ccgttcctcg gcaaagcggc tggcgatggc
     5341 gttataacgg cttaaatcga gatcgataat cgtcacttca cctgggccgc acggctgttc
     5401 ttttttgcgc ggagttgaag attgtatcaa ccgggcgggt agataacgcg cttccgccag
     5461 cagaaaccag caaatctgcg cgacgtgggt gccggtggtg atgtgaatta aatagtcttc
     5521 tttttctggc tgaaactcgt aaccacgggc gaaatcatgc aggcaggcgt aaacctcttc
     5581 gaaatcccac gggttatgca gttcaatctc aacgcccacc acttctgttt ctggcgaaac
     5641 gctggcgata tcgcgtttaa gcgtttcaaa tagcgagcgc gagcgggcat cgtgcaataa
     5701 ttccagtcga tcgatgacca acgattcttg ctggcatata cagagtgacg gacgccattt
     5761 tgaccagcgc tgactgccgc gtccggcata atccagcacg gtaccgacaa agccaaaagc
     5821 cactgtttta cgcatcttag atatccttat aaaaggcgat atttcattat cggtaaatcg
     5881 gctttgcggg aaatctccgt ttacggacat aaagatataa tctttttaga aacaataaat
     5941 taaaaaatta atctgcttat ttaattttct ggcacga
//
LOCUS       DAJVQH010000181         5946 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJVQH010000181 DAJVQH010000000
VERSION     DAJVQH010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.182"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..1112
                     /locus_tag="OQM87_003868"
     CDS             <1..1112
                     /locus_tag="OQM87_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCS1509813.1"
                     /translation="WPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMA
                     VAIQHGSFIEDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTDQ
                     ALNAEIQKGEEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRD
                     PFDWAEKNLQEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVF
                     IDNYAAIPSESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFID
                     ARGQRPLKVKDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHD
                     QPFVQGLTACAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(1124..1936)
                     /locus_tag="OQM87_003869"
     CDS             complement(1124..1936)
                     /locus_tag="OQM87_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509814.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(1940..2725)
                     /locus_tag="OQM87_003870"
                     /pseudo
     CDS             complement(1940..2725)
                     /locus_tag="OQM87_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
     gene            complement(2722..3390)
                     /locus_tag="OQM87_003871"
     CDS             complement(2722..3390)
                     /locus_tag="OQM87_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS1509815.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(3454..4092)
                     /locus_tag="OQM87_003872"
                     /pseudo
     CDS             complement(3454..4092)
                     /locus_tag="OQM87_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
     gene            complement(4105..>5946)
                     /locus_tag="OQM87_003873"
     CDS             complement(4105..>5946)
                     /locus_tag="OQM87_003873"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCS1509816.1"
                     /translation="KFSRDAHMGGFFAAQMKFAGYDVIIIEGKAKSPVWLKIKDDKVS
                     LEKADFLWGKGTRATTEEICRLTSRETCVAAIGQAGENLIPLSGMLNSRNHSGGAGTG
                     AIMGSKNLKAIAVEGTKGVNIADRQEMKRLNDYMMTELIGANNNHVVPSTPQSWAEYS
                     DPKSRWTARKGLFWGAAEGGPIETGEIPPGNQNTVGFRTYKSVFDLGPAAEKYTVKMS
                     GCHSCPIRCMTQMNIPRVKEFGVPSTGGNTCVANFVHTTIFPNGPKDFEDKDDGRVIG
                     NLVGLNLFDDYGLWCNYGQLHRDFTYCYSKGVFKRVLPAEEYAEIRWDQLEAGDVNFI
                     KDFYYRLAHRVGELSHLAYGSYAIAERWNLGKEYWGYAKNKLWSPFGYPVHHANEASA
                     QVGFIVNCMFNRDCMTHTHINFIGSGLPLKLQREVAKELFGSEDAYDETKNYTPINDA
                     KIKYAKWSLLRVCLHNAVTLCNWVWPMTVSPLKSRNYRGDLALEAKFFKAITGEEMTQ
                     EKLDLAAERIFTLHRAYTVKPMQTKDMRNEHDLICSWVFDKDPQIPVFTEGTDKMDRD
                     DMHASLTMFYKEMGWDPQLGCPTRETLQRLGLEDIAADLAAHNLLPA"
BASE COUNT         1509 a         1596 c         1367 g         1474 t
ORIGIN      
        1 tctggcctga tgaagaagaa gcaaccagaa cgtattttcc cagcccgtgg tcaggcctga
       61 tggaagcaaa ggtcgatgcg tgtaacgtgg gtattatggg aacatccttg agcggactgg
      121 atgcggcaat ggcagtggct attcagcatg gttcgttcat tgaagatgat aaacaacacg
      181 tcgtttttca ccgcgataac gcaagtgaaa agctaaatat tacgttgatg tcgcgcacgg
      241 gtattttacc cgaagccgat ttctattgcc ctattccctg cgagccctta cacatcgtca
      301 ccgatcaggc attaaatgct gagattcaaa aaggtgaaga gggccttttg gatcgggtat
      361 ttagattgat agtagaggaa atcaagtttg ctgatccaga ctggagccaa cgcatagcct
      421 tagagagcct gaatgtcgat tcctttgctc aagcctggtt tgccgagcgc aaacaacgcg
      481 acccatttga ctgggcagaa aaaaatctcc aggaagtcga acgcaataaa cgagaaaaac
      541 atactgttcc ctggcgttat gtcattctgc gcctgcatga agccgtacag gaaattgttc
      601 ctcatctgaa tgaacacgac cataaacggt tcagtaaagg ccttgccagg gttttcatcg
      661 ataattatgc ggcaatccct tcagagtcta ttcgtcgact actggcctta cgtgaagcgg
      721 ggatcattca tattctcgcc ctcggtgaag actacgaaat ggaaattaat gagtcgcgca
      781 ccgtcctgaa aacggaagac aacagctact cgtttgacgt ttttattgat gcccgcggac
      841 agcgtccgct taaagtgaaa gatattcctt tccctgggct acgcgagcaa ttacagaaaa
      901 caggggatga aatccctgat gttggcgaag attatacgtt acagcaaccc gaagatattc
      961 gtggacgcgt agcgttcggc gcgttgccct ggctgatgca cgaccagcct ttcgttcagg
     1021 gacttacggc atgtgcagaa attggtgagg cgatggctcg ggcggtcgta aagcctgcat
     1081 cccgtgctcg tcggcgtctt tcgtttgatt aaaggtaaag ctatcagcac tcaacaaact
     1141 gatgctgttc attaagacga tagcggactc cggttcgaat attgttttca ccttcaatgg
     1201 cgacggcgaa gcgaactctt tcgccgtcat gatatgccag tgcggcgctt ccccctagcc
     1261 cgagaataat tgagtcgacc acgcccgtgc tcactaccgt tgaattttca ccgctggcaa
     1321 tgatgtgtac attgtcgccg ctgttggcaa ttctggcgtt tgcgccaaag ctggcgattt
     1381 gcaccaggtc accattactg gcgacatgac accgttcacc caatgtacaa acgcgtactc
     1441 gcattccggt attcgcaatc cgactactat ctccagcgct gcttatccgc gaagaattac
     1501 cggttacagc aacccgtgcg cgttcaccgg agctgccaat gtgactgtta aagccgacac
     1561 ttccaatccg cacgctataa cccatactgg caatttgcgc ggcataccca gcggttgcga
     1621 ttcgggcgtt atcctcacaa cagccgatgc gagcatgatc gtcgctgcgg gcaaactgac
     1681 tgcaaaatgg tatctctccg gggtctgtgg ccagtttttg catagaggaa acttcctgat
     1741 gcgcgaagtt ttcatccgcc agccattttt tccacgcata ctcaaccaga ctctccgccc
     1801 agtccgtata tccttcctca atcagtgcat gacgaatatc ggcataacta ccgccgcgtg
     1861 ggaaatggcg caggaaccag cgatacatca ccgctccgat acgccattct cgcaggtctg
     1921 ctcgtgtaat gatcattcgt tagtgccggt gatagccatc gaccatgctt ttaaaggttt
     1981 cgtgtggcgt acgccccgtg gtgcaaagat aaagatgacc gaagataaaa aagaggctta
     2041 taaatgccag agcaaaatgc gcctgcaata accagtatct tacgccagga aacacatctc
     2101 ccacggcttg cggatagaga cacaacagcc ccgtcaatag taacaacggc agcaatccat
     2161 acatgacacc aacataggcg acctgctgta agggattaaa tttagactgt gttgttgccg
     2221 ggaaaggatg ttcttccccc tgcataatgc caaacaaata aaatcgcgtt tgttttgccg
     2281 ctcgttccag ccacccctga cgacgaatgc gatagtggtg accattatcc ccaacggcat
     2341 tgatcagcac aaagccgagc cagcatgcca gtaacaaaaa tcagcaaact tcatgcaccg
     2401 caaccagact tttaaccgca gttgcgccca ccatcgcaaa gtgatttatc agcccacttg
     2461 ccagcaacaa tacaaagagt aacgcattcg accagtgcca taatctgacc gctttgctgt
     2521 aaagataaac cttctcacca tgatcggtgg ctgatttctt cacgccacga gcacgaagca
     2581 aggcgtgcag ccccagcacc aaccacatcc caaccagcaa tactccggca atgatcaacc
     2641 acaccggcca aaattcggga gtgaactgtg gcacatagtt cgctaactgg ctctgaaact
     2701 gttcagcatg ttgcgacggg ttcatacatt ttccttttta atcaaatgtt gaccgaaccg
     2761 gcgatacaga tgcggttttc cggcaccagg tagctgatat tgatagtatt tattatcctg
     2821 caaccacgcc tgaatttctg ggctatcttc gcgaccaaag atcaatgcgt gttccggaca
     2881 ggcgctgacg caaatgggcg gaaagccttt tgccagtcgg gactcagcgc agaaatcaca
     2941 tttatctgcc actttagtga cagggttaag gtagcgaacc tggtacggac aagcgccaat
     3001 gcaataactg cagccgatgc actgcgattt ttctacccgc acgatcccct gctcatcacg
     3061 ccacgatgcc cccgtcgggc atacgtcgat gcacggagca tcttcgcagt gctgacatga
     3121 ctggcgaaaa aagtgatact gcgtttcgtt gtcgttatcg gtgactggaa tgtgtgctat
     3181 cgataaacgg cttccctgag cggggacgtg gttagtttta cggcatgcgc gggcgcaaat
     3241 attacagcca ttgcaccgtg actcgtcatg aatcatggca taccgaactt ccttttcttg
     3301 cctcgtgttc gctaacagag aagacgcaga accagtaaaa aagattactg ttcccatccc
     3361 cagaacaaat ttgcgtcgag tgaaggacat ctgattatcc cctaatgcag tggctgcaac
     3421 tgtccaccct atccatgaca tctccctgaa atttcagggg ttctgaaact gctctacctc
     3481 gaaccattcg cataagccat aagccttgcc ataggtcata aatcgctcgc taaacaagta
     3541 caacaccgcc gtcgatgact ccacctgccg tatatcggca tattcaggtg caacatccat
     3601 cgcggcggtc gcggcttcaa tctgcgcttc ctagaagtaa tatggcgctt gcatcagcat
     3661 ggcgactttg taaggacgag gatacgtctg gcactcaaaa cgaacggcgt gtgcaatggc
     3721 gcgacaaatg tcctgctcca caacttgcaa agacatcgcc gcgtagtttt cactcatggc
     3781 ctgcgttgag taatagtaat catcctgact tcccgtcata caagcgatat ccgcgtagtc
     3841 tccgttctgc ttaatgcttt ccagcaaaac tgacagttcc tcttctgcaa ccgaataggg
     3901 tggcgagaga aacacggtgc gcgccaccag ctgcccagcc gcagaatgct ggcgaatgaa
     3961 ctcagccagg atttcagcag gttctggttc ggttaccgta tcaacggggt gacgcatccc
     4021 ctgcaaccat tgccgcttcg cctcgtcaac ttcgctgact ttcgctaacg gcaattcatc
     4081 ctgatggttc atcttaccca ttccttacgc aggcagtaga ttgtgtgctg ccagatcggc
     4141 ggcaatatct tccagcccca gacgctgcaa tgtttcgcgg gttggacaac caagctgtgg
     4201 gtcccagccc atttctttgt agaacatcgt gagcgaagca tgcatatcgt cacgatccat
     4261 tttgtcagta ccttcagtaa agaccgggat ctgcggatcc ttgtcgaaaa cccaggaaca
     4321 gataagatcg tgttcattac gcatatcttt ggtttgcatc ggttttaccg tgtaggcacg
     4381 atgcaacgta aagatacgct ctgcagctaa atctaatttt tcctgagtca tctcttcgcc
     4441 ggtgatcgct ttgaagaatt tggcttcaag cgccagatcg ccccggtaat tacggctttt
     4501 cagcggcgaa acggtcattg gccagaccca gttgcacagg gtgacggcgt tatgcaaaca
     4561 gacccgcaac agcgaccact ttgcatattt aatttttgcg tcattgattg gcgtgtagtt
     4621 tttggtttca tcgtaagcat cttcagaacc aaaaagttct ttcgccactt cacgttgcag
     4681 tttcaatggc aagccggaac caataaagtt gatatgggta tgcgtcatgc agtcacggtt
     4741 gaacatacag ttaacaatga aaccgacctg cgctgacgct tcattggcat ggtgaaccgg
     4801 atagccaaac ggcgaccaga gtttattttt cgcgtagccc cagtactctt tacccaaatt
     4861 ccagcgttct gcgatggcat atgaaccata agccaggtga ctcagctcac ccacacgatg
     4921 cgccagacgg tagtaaaaat ctttaatgaa gttaacgtca cccgcttcca gttgatccca
     4981 gcgaatttct gcatactctt cagctggcag aacacgcttg aacacacctt tgctgtaaca
     5041 ataagtaaag tcgcgatgca actgcccgta gttacaccat aggccgtagt catcgaacaa
     5101 attcagacca accaggttac caatcacacg accatcgtct ttatcttcaa aatctttcgg
     5161 cccgttcggg aagatggtgg tatggacaaa gtttgctaca caagtgttac cacctgtgct
     5221 gggcacgcca aactctttca cccgaggaat attcatttgg gtcatacaac ggatcgggca
     5281 agagtggcag ccgctcattt ttactgtgta tttctctgcc gccggtccta agtcaaaaac
     5341 ggatttatag gtacgaaagc cgaccgtatt ttgattgcct ggcggaattt cacccgtttc
     5401 aatcggacca ccttcagccg cgccccaaaa cagcccttta cgtgctgtcc agcgtgactt
     5461 gggatctgaa tactctgccc acgattgtgg cgtacttggc acgacatggt tgttattcgc
     5521 accaataagt tcagtcatca tgtaatcatt gagacgcttc atctcctgac gatcggcaat
     5581 gttgacccct ttcgtccctt caaccgcaat cgctttcagg tttttcgaac ccattattgc
     5641 gccagttccc gcaccgccgc tgtggttacg gctattcaac atgccagaga gaggaataag
     5701 gttttcccca gcctgaccaa tagccgccac acaggtttcc cgactggtca atcgacaaat
     5761 ttcttccgtc gttgcgcgcg tcccttttcc ccataagaaa tcggcttttt ccaggctaac
     5821 tttgtcatct ttaatcttca gccataccgg tgatttcgcc ttcccttcga taataatgac
     5881 gtcgtatcca gcgaatttca tttgcgctgc aaaaaagcca cccatatggg catcgcgact
     5941 aaattt
//
LOCUS       DAJVQH010000182         5779 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJVQH010000182 DAJVQH010000000
VERSION     DAJVQH010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.183"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            121..639
                     /gene="mddA"
                     /locus_tag="OQM87_003874"
     CDS             121..639
                     /gene="mddA"
                     /locus_tag="OQM87_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCS1509817.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            636..1085
                     /locus_tag="OQM87_003875"
     CDS             636..1085
                     /locus_tag="OQM87_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1509818.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1086..1318)
                     /locus_tag="OQM87_003876"
                     /pseudo
     CDS             complement(1086..1318)
                     /locus_tag="OQM87_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1404..1577)
                     /gene="ortT"
                     /locus_tag="OQM87_003877"
     CDS             complement(1404..1577)
                     /gene="ortT"
                     /locus_tag="OQM87_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCS1509819.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1772..1867
                     /gene="yncL"
                     /locus_tag="OQM87_003878"
     CDS             1772..1867
                     /gene="yncL"
                     /locus_tag="OQM87_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCS1509820.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2269..3078)
                     /locus_tag="OQM87_003879"
     CDS             complement(2269..3078)
                     /locus_tag="OQM87_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCS1509821.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3288..4713)
                     /gene="patD"
                     /locus_tag="OQM87_003880"
                     /pseudo
     CDS             complement(3288..4713)
                     /gene="patD"
                     /locus_tag="OQM87_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4735..5441)
                     /locus_tag="OQM87_003881"
                     /pseudo
     CDS             complement(4735..5441)
                     /locus_tag="OQM87_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(5431..>5779)
                     /locus_tag="OQM87_003882"
     CDS             complement(5431..>5779)
                     /locus_tag="OQM87_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCS1509822.1"
                     /translation="MILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIA
                     AGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALY
                     LAFVKRLGAFDAL"
BASE COUNT         1513 a         1457 c         1284 g         1525 t
ORIGIN      
        1 ctatcactta aataagtgat actggttgtc tggagattca gggggccagt ctatccgttg
       61 cttcgggctg ttttcgatag tattgataac atcatcatca ttattttatt gaggccagac
      121 atgtccatcc gttttgcccg caaagccgac tgtgctgcca ttgcggaaat ttataaccac
      181 gccgtgttgt atacggcggc tatctggaat gaccaaacgg tggatgcaga taaccgcatt
      241 gcctggtttg aagcacggac catagcaggt tatccggtgc tggtaagtga agaagatggc
      301 gtagttacgg gatatgcctc gtttggcgac tggcgtagtt tcgatggttt tcgccatacc
      361 gtggaacatt cggtttatgt ccatcccgat catcagggca aaggtctggg gcgtaaattg
      421 ttaagccgat tgattgatga agcgcgggat tgcgggaagc atgtcatggt cgccgggatc
      481 gaatcgcaaa atcaggcttc gctgcatctg caccagtcgc tgggatttgt cgtcaccgca
      541 caaatgccgc aggtaggcac taaatttggt cgttggctgg atctgacatt tatgcagttg
      601 caactcgacg agcgcactga accggacgcg attggatgaa tcagtcgctc acccttgctt
      661 ttttgattgc cgccggaatt ggtctggtag tccagaacac gctgatggtg cgcatcaccc
      721 agacctcctc taccattctc atcgccatgt tgctgaactc actggtgggg attgtgctct
      781 ttgtttctat tttatggttt aaacaaggca tggcggggtt tggcgaactg gtgtccagcg
      841 tgcgctggtg gacacttata cctggcttgc tgggttcgtt ttttgtcttc gccagtatca
      901 gcggatatca gaatgtggga gcggcgacaa ctattgcggt actggtcgcc agccagctga
      961 ttggcggtct ggtgctggat atcttccgca gtcatggcgt accactacgg gcgttgttcg
     1021 gcccaatatg tggcgcaatt ttacttgtgg ttggcgcctg gttggtggcc agacgctcat
     1081 tttaatcaga ggatggtgcc gcctttggtt aactgttcgt ggccgcttcc atatcttctt
     1141 tatgcttgcg accgtgatga gcaatagcgg tgcgcaaacg ctgctgctgg gtgtagcgat
     1201 cttcccgact taactccgcg tcatcgctca gggcgattaa taattcgttc atatgggcaa
     1261 tgacgctctc ttcaatcgcg gcatccacgc gcgcgatgac ttcatccaga tgcgacatac
     1321 tttctcctta gccacttgtg aaatttatcg attaatccaa atgatataag tctgcgtggt
     1381 gccgtcaagc cagtgagtaa gatttaaaac agtgaaaaca acagcgccac cgggaaactc
     1441 atcggccatg tggcccccac cagaaaggcg cttaagaaac ggatgcgttt cttatcgtga
     1501 gaaagaaacc aggtgataag aaatgcgatt gctgccataa ccgcataaaa aacaagcata
     1561 tgttgataga gagacatgta cgggcgtcca gccatatgaa taaaacgcgc gcaaatatgc
     1621 gccatttttt gacgtgcatc aagttttttc accagtttga gaaaaaatca tcaaacagca
     1681 acttgccctt atccatcttc agaatgagta ttattgcgga ttttcttaac ccgtactata
     1741 cacatagggg caatgataaa aggtggcaaa aatgaatgtt tccagtagaa ctgtagtact
     1801 gataaatgtc tttgctgctg ttggtttgtt tactcttatc tctatgagat ttggctggtt
     1861 tatttgatgt agtgattttg cacgcagcac gtactgtcag agaaatcaac tctctgacag
     1921 tacgcaagat cttctcatac tctccaagtt tgcaacatta tccttaatat ttacttcttt
     1981 ttgctggcat ctacattgaa atgagtagaa aacatccccc tactgtcgtc tatacaaaca
     2041 gcttactgat tcaaacggaa tcttgttttc acatttttct atcacgaata ctttatacgc
     2101 gcgggagtct ataaatgctt tatactttca aggaataacg taaatataaa aagatggcgt
     2161 aaagtatatg catggcagga tgtttgcaac ctccttgatg acgctgcccc attgaaattg
     2221 caaaccccgc cctggttatc ttgcccattt atcatgcaaa acagataatt ataggcctct
     2281 gaatacctca gaaagattga attttttata gtccctctct tgaatatttc catttgatat
     2341 caatcgattc atctcttcaa caaaataatt tatatcatca cacttgttat tatccggaag
     2401 atctgagtag ttataattcc accattttat tctcaacagt tcatcaataa catttgaatc
     2461 aaacctaaat ctgattattt ttgcaggaac tcctgcaact atggcatatg ggggcacatc
     2521 tttggtaaca acggaattgg cagcaataac agcaccatca cctattgcga tgccaccttt
     2581 caatacaaca tcatttccta tccagacatc atttcctata atcggatttt caggtttggt
     2641 aatgaatggt ttcaatgtcc agctacgttc aaattcactc atagcaaact tatcaaattc
     2701 accgttataa gtcatcatgt gagtagtaaa acgattcaaa ggatgatttc cacccattat
     2761 ttttacattt gatgcaatac tacaaaatcg acctatacgt atattccctg aaaaatggca
     2821 ggtcggaaat gaaaaagcgc cgacactgga gaaatttttc acaggcattg ttgaatattc
     2881 ttcagcaata acattatctt caattgttat cttgtcacct tttgagaaaa gaaataaatt
     2941 cctttcttta tacgacttct tccccttatg atttaaaaag attttattat ccatacaaaa
     3001 ctcaaagtgt tttttcgtcc atctgaaggt aagggtttta tttttatctt ccgtgaattt
     3061 gtacaattga actttcattg ttaatacttc tacttataaa ttattagttt aataacgata
     3121 ctctaaagcc ttatgctaat caactgtgac aggatgaaaa atcgccatca aacatttagc
     3181 ctaattttta cgccagttat taatgtaatc attctaatac ttaaccgcca ttaaactcag
     3241 aacctaaaac gatagtggcc gtcctataac agccactaac atctaactta atgtttaacc
     3301 atgacgtggc ggacgacggt gtaatcctcc agcccataaa gtgacatatc cttgccgtaa
     3361 ccagaaagtt tctgcccacc gtgcggcatt tcacttacca gcatgaaatg ggtattgacc
     3421 caggtacaac catattgcag ccgtgcgctg acgcgatgcg ccctgcccac atctttcgtc
     3481 cataccgaag atgcaagtcc gtactggctg tcattcgccc agttcaccac ctgttcttcg
     3541 ttgtcgaagg gcgtaacact cactactgga ccaaatacct ctttttgcag gatggcatcg
     3601 tcctgtaatg cgccagccag cagcgtcggc gcatagtaat agccattacc cttgcgcttt
     3661 tcaccgccag tgatcacttt gatgtgccct gtcgctttcg cctcttctac tgccttgctg
     3721 acgcgttcga gatgcgccag cgagcttaaa ggtccaagct ccgtagactc gtcatccggc
     3781 gcaccagatt ttaacgttgc caccgcaggc acccagtttt tccaccagcg tatcgtaaat
     3841 gcctttttgc gcgtagatcc gacaagccgc agtacaatcc tgtccagcat tgtaatagcc
     3901 aaatgtacgt acaccttcga ccactgcttc aatatccgca tcatcaaaaa caatcactgg
     3961 cgctttgcca ccaagttcca tatgagtacg cttaatggac gacgcggtat ggctgatgat
     4021 gtgctcgccg gtggcgatag agcccgtcag cgacaccatc cgcactttgg gatgaccggt
     4081 cagcggatca cccaccgttt tgcctctgcc aaacagtatg ttaatcacgc ctgccgggaa
     4141 gatatctttc gccagctctg ccaacttcaa cgcggtcagc ggggtaattt ctgatggttt
     4201 aagcactacg cagttccctg ccgccagcgc cggagcaagt ttccacgcgg ccatcatcag
     4261 cggataattc cacggtgcga tagaagccac gccccccaac ggatcgcgac ggatcatcga
     4321 agtatgacct tcaaaatatt cacctgccgc cagaccattc agacagcgcg ccgcacccgc
     4381 gaaaaagcga aaaacatcga caatcgccgg gatttcatca ttgaacgcac tatgcagcgg
     4441 tttgccacaa ttacgggatt ccagttcggc aaaaacctga ccattttctt cgataacatc
     4501 cgccagtttc agcaggcatt ccgcacgcgc tttcggtgtg gtttgccccc attcagcaaa
     4561 tgctgcatct gccgcacaca cagcagcatc gacctgctct gcggatgcct cggcaatctc
     4621 cagtaaaacg tcccccgttg ccggattata gacaggctgt ttttcccctt cgccgctaac
     4681 cagttctccg ttaatcagta acttatgttg catagattat tcctgtatca gtggttattg
     4741 accattgtcg ccttcgcggg ttagccacca ggcccccagg atcggcaagg ttgttaccaa
     4801 cataaccagc agtgccacca cgttggttac cggtacatca cgcggtcgcc caagctgatt
     4861 gagcaaccac aacggtaacg ttcgttcatg acctgccgta aaggtcgtaa cgatgatttc
     4921 atcgaacgac aaggcaaacg ccagcattcc tcctgccagt aacgccgaac tgagattcgg
     4981 caacactacg tagcggaagg tttgccagcc attggcccca agatccattg acgcctcaac
     5041 cagactccac gaggtgcggc gaaaacgggc gatgacattg ttaaacacca ccactacgca
     5101 aaaagtcgca tgaccgacca cgatggtgaa aaatcccggc tccagattga tggttttaaa
     5161 ggcggttaat agcgccagac cagtgacaat gcccggcagc gcaatgggca gcagcaataa
     5221 cagcgaaatg gcgtttttgc cgaaaaagtc tcgtctccac agcgcggcag ctgccagcgt
     5281 ccctaacacc agcgcagcgt caggccctgc ggtggaaaac taaacgccgc atcttcagtg
     5341 ttaaacgcat aggcggcgat tatcaggatg ggaaaatgta ggaaaacaac gccgccccag
     5401 gccgccagtt tgaggaaaaa cggtgcgcgt tcagagtgca tcgaacgctc ccagacgttt
     5461 cacgaacgcc aggtacagtg cgatgagaat aatcggcacc agggtgaatg ccgccgccat
     5521 cggcatattg ccaatcgccc cctgctggga ataaaccata ttgccgataa aatatcctgg
     5581 aggaccaacc agctgcggga cgataaaatc gcccagtgtg agtgagaagg taaagataga
     5641 gccagcggca atacccggga ttgccagcgg cagcaccaca tagcgaaagg tttgtcgtgg
     5701 acgtgcgccg agatcagccg acgcctgcaa caatgacggc ggcaaacgct caagcgccgc
     5761 ctgaacgggc aggatcatc
//
LOCUS       DAJVQH010000183         5599 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJVQH010000183 DAJVQH010000000
VERSION     DAJVQH010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.185"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..410
                     /locus_tag="OQM87_003883"
     CDS             <1..410
                     /locus_tag="OQM87_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1509823.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /gene="istB"
                     /locus_tag="OQM87_003884"
                     /pseudo
     CDS             442..1056
                     /gene="istB"
                     /locus_tag="OQM87_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="OQM87_003885"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="OQM87_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCS1509824.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWFSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1380 a         1402 c         1329 g         1488 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggacagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tcaacagcgc aggagagctg
      721 ttgcaggaac tacataaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag gcctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggtata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggtc ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagaa
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gcccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc gacgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaagaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tgctcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aagggccagt ttggatatc
//
LOCUS       DAJVQH010000184         5587 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJVQH010000184 DAJVQH010000000
VERSION     DAJVQH010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.186"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(296..580)
                     /locus_tag="OQM87_003886"
     CDS             complement(296..580)
                     /locus_tag="OQM87_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCS1509825.1"
                     /translation="MLDMAGLRADILSPVKETGTLLGVVSSQAAELCGLKAGTPVVVG
                     GGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQLPITPFRMPS"
     gene            614..1420
                     /locus_tag="OQM87_003887"
                     /pseudo
     CDS             614..1420
                     /locus_tag="OQM87_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            1432..2455
                     /gene="lsrB"
                     /locus_tag="OQM87_003888"
                     /pseudo
     CDS             1432..2455
                     /gene="lsrB"
                     /locus_tag="OQM87_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            2482..3357
                     /gene="lsrF"
                     /locus_tag="OQM87_003889"
     CDS             2482..3357
                     /gene="lsrF"
                     /locus_tag="OQM87_003889"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCS1509826.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PGVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            3381..3671
                     /gene="lsrG"
                     /locus_tag="OQM87_003890"
     CDS             3381..3671
                     /gene="lsrG"
                     /locus_tag="OQM87_003890"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCS1509827.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            3710..4468
                     /gene="tam"
                     /locus_tag="OQM87_003891"
     CDS             3710..4468
                     /gene="tam"
                     /locus_tag="OQM87_003891"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCS1509828.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(4472..5386)
                     /locus_tag="OQM87_003892"
     CDS             complement(4472..5386)
                     /locus_tag="OQM87_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCS1509829.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNELPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         1406 a         1341 c         1484 g         1356 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt
      661 actgtttcaa agtggtgtgc cgatgccgct ggcgatactc ctgaccttac tgctcggcgc
      721 attgtgcggg ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat
      781 tacgcttggc acgctgtatc tgtttgccgg aagcgctctg ctgctttccg gtatggccgg
      841 agcgacgggg tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct
      901 ggatgtgctg ggactccccg ttccgctgat tatcttcctg atatgtctcc tcgttttctg
      961 gctctggctg cataaaaccc atgccggacg taatgtgttt ttgattgggc aaagcccgcg
     1021 cgtggcgctt tatagcgcga ttccagttaa ccgcacctta tgtgcgctct atgccatgac
     1081 ggggctggcg tctgcggtcg ccgctgtgct gctggtatcg tattttggtt cagcacgttc
     1141 cgatctcggt gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg gcggggccaa
     1201 tatttatggt ggttccggtt ccattatcgg caccgccatt gcggttttat tagtgggata
     1261 tttgcagcaa ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg
     1321 tgcgctgctt atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa
     1381 agagtggctg gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt
     1441 catcgcttta agaaaatcgc cttacttagc gctcttggca ttgccgcaat ctctatgaat
     1501 gtgcaggccg cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc
     1561 agcggtggca acggcgcaca acaagcgggt aaagagctga gcgttgatgt gacctacgac
     1621 gggccgacag aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcaa
     1681 ggttataacg ccattattcg tttctgcggt ttcgcctgat ggcttgtgtc cggcactgaa
     1741 acgcgccatg caacgtggtg tgagagtgct gacctgggac tctgatacta aaccggagtg
     1801 ccgctcttac tacattaatc agggaacgcc cgcccagttg ggaggtatgt tggtggatat
     1861 ggcggcgcgt caggtgaata aagacaaagc caaagtcgcg tttttctact caagccccac
     1921 cgttacggac caaaaccagt gggtgaaaga agcgaaagcg aaaatcgcca aagagcatcc
     1981 aggctgggaa attgtcacta cgcagtttgg ctataacgat gccactaaat cgttacaaac
     2041 cgcagaagga atattaaaag cgtatagcga tctcgacgcc attatcgccc ccgatgccaa
     2101 cgccctgccc gctgccgcac aagccgcaga aaacctgaaa aatgacaaag tagcgattgt
     2161 cggattcagt acgccaaatg tgatgcgccc gtatgtagag cgcggcacgg tgaaagaatt
     2221 tggactgtgg gatgtggttc agcaaggcaa aatttcagtg tatgtcgcgg atgcattatt
     2281 gaaaaaagga tcaatgaaaa cgggcgacaa gctggatatc cagggcgtag gtcaggttga
     2341 agtctcgcca aacagcgttc agggctatga ctacgaagcg gatggtaatg gcatcgtact
     2401 gttaccggag cgcgtgatat tcaacaaaga gaatatcggc aaatacgatt tctgatgtgc
     2461 attacttaac cggagtaagt tatggcagat ttagacgata ttaaagatgg taaagatttt
     2521 cgtaccgatc aaccgcaaca aaatatccct tttaccctga aaggttgcgg tgcgctggat
     2581 tggggaatgc agtcacgctt atcgtggata tttaatccga aaacgggtaa aactgtgatg
     2641 ctggcctttg accatggtta ttttcagggg ccgactaccg gacttgaacg cattgatata
     2701 aatatcgccc cgctgtttga acatgccgat gtattaatgt gtacgcgcgg cattttgcgc
     2761 agcgtagttc ccccggcgac caataagccg ggggtactgc gggcgtcagg tgcgaactct
     2821 attctggcgg aattaagtaa tgaagccgtg gcgttatcga tggatgatgc cgtgcgcctg
     2881 aacagttgcg cggtggcggc gcaggtttat atcggcagcg aatatgaaca tcagtcgatc
     2941 aaaaatatta ttcagctggt cgatgccgga atgaaagtgg gaatgccgac catggccgtg
     3001 actggcgtgg gcaaagatat ggtgcgcgat cagcgttatt tctcgctcgc gactcgaatc
     3061 gccgctgaaa tgggggcgca aattatcaaa acctattatg tcgaaaaagg ttttgaacgg
     3121 attgttgccg gatgtccggt acccattgtt attgctggcg gtaaaaaatt accggagcgc
     3181 gaggcgctgg aaatgtgctg gcaggctatc gatcagggcg cttctggtgt ggatatgggg
     3241 cgtaatattt tccagtctga ccatccggtg gcgatgatga aagccgtaca ggcggtggtt
     3301 caccataacg aaacggctga tcgggcatat gaactctatc tgagtgaaaa acagtaactg
     3361 cggatttaag gagaagaatt atgcacgtca cactggttga aattaacgtt catgaagaca
     3421 aggttgacga gtttatcgaa gtttttcgcc agaaccacct gggctctgta caggaagaag
     3481 gtaatttgcg cttcgatgtc ttacaggacc cggaagtgaa ttcgcgcttt tatatttacg
     3541 aagcctataa agatgaagac acagtggcat tccataaaac cacgcctcac tacaaaacct
     3601 gtgtcgcgaa actggaatct ttaatgaccg ggccgcgtaa aaaacgtctg ttcaatgggt
     3661 tgatgccgtg aggctacaat tggggtaacg cgctgacggg agtaaaaaaa tgtctgactg
     3721 gaacccctct ttatatctgc acttttccgc tgaacgatcg cgtccggcgg tggagctgct
     3781 tgccagagtg ccgctggaga atgtcgaata tgttgccgat cttggctgtg gcccaggtaa
     3841 cagcaccgcc ctgctgcatc aacgttggcc tgcggccagg ataacaggca tcgactcgtc
     3901 tccggcgatg attgctgaag cgcgcagtgc tttgccagac tgccagttcg tggaagcaga
     3961 catccgcaac tggcaaccgg aacaggcact cgatctgatt tttgctaatg cctcactgca
     4021 atggctgccc gaccactacg aattgtttcc tcacttggtt tctttactta atccgcaggg
     4081 cgtgctggca gtacagatgc cagataactg gctggagccg acccatgtgc tcatgcgcga
     4141 agttgcttgg gaacaaaact acccggatcg cgggcgtgag ccgttggctg gcgttcatgc
     4201 ttactacgat attttgagcg aagccggatg tgaggtcgat atctggcgaa ccacttacta
     4261 tcaccagatg ccgtcgcacc aggcgattat cgattgggtg accgccactg gattacgtcc
     4321 gtggttacag gatctgaccg agagcgaaca gcagcttttt cttacgcgct accatcagat
     4381 gctggaagag cagtatccac tgcaagagaa cggacagata ctgctggcat ttccgcgtct
     4441 gtttattgtt gcccgccgta cggagtaatt atcacgtcag ctggtaatga cgatcgggaa
     4501 gaatttttgc tggaatttct gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt
     4561 tgcagatggc atccagcggt aactcattgt tttcagtacc gaacggatct tccagttctt
     4621 ccgccagaca atccagcgaa ataaaagtgt aggaaatcag cacagagata aaaggcgtca
     4681 tgtaatgcag gtccacgacc agcgcgaacg gcagcatgat acaaaacaga taaacggtac
     4741 gatgcaaaat cagggtgtag gcaaagggaa ttggcgtgta ggcaatgcgc tcacagcctg
     4801 ccagaaccgc tgaaatatca ttaagccgat cgttgaggct aataaacagg atatctgaaa
     4861 gctgtccatt gcggcgctga accgccaacc attctcccat tattaacaag atacggttag
     4921 ctggagagtt cgaagccagt acacgctgaa gatctccagt cttgagataa tgagccagca
     4981 cttccgcctg tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca
     5041 gcctggcaaa ctcccttaca cttgccgaat ccggcaatgt cgtttttacc tctcgcagta
     5101 acgaccgtga ggcaatcatc aactgccccc aaagttttcg cgcttcaacg taacgggcgt
     5161 acccggcatt attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg
     5221 gtgcgagggt gaatttgatg cccagatgcg tgtaccaggg cagcatgaat ataacagcaa
     5281 tagaaaagag aaaattgagt agtaagcgcg aggatatctt ggataatact gagccgtgcc
     5341 agacaaaaat acggcgcagc cagtgttgtt gtggacgaac aatcatggtt atcttcaggc
     5401 gtggaaaagt cgccctatta aacgtgatta cgatcacatt ctcaagacgc tacttacaaa
     5461 ttacctagaa ccacggcagc ctgacggctg caaattgaaa accgcccctg ttcgtcctgt
     5521 tgttgccgca cgctccgcag aatatgtatg ctctgaggac ctgttttatt aaggactcat
     5581 cctctta
//
LOCUS       DAJVQH010000185         5544 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJVQH010000185 DAJVQH010000000
VERSION     DAJVQH010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.187"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..628
                     /locus_tag="OQM87_003893"
     CDS             <1..628
                     /locus_tag="OQM87_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCS1509830.1"
                     /translation="IVLMELMMMVQLWWSVWLCWPVLSLLIVGRGCGGLGAITTGKDS
                     QGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAVLDV
                     TFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSR
                     SALVVAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(682..1287)
                     /gene="azoR"
                     /locus_tag="OQM87_003894"
     CDS             complement(682..1287)
                     /gene="azoR"
                     /locus_tag="OQM87_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCS1509831.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1488..5390
                     /gene="hrpA"
                     /locus_tag="OQM87_003895"
     CDS             1488..5390
                     /gene="hrpA"
                     /locus_tag="OQM87_003895"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCS1509832.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLSFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1376 a         1354 c         1535 g         1279 t
ORIGIN      
        1 cattgtgttg atggagctaa tgatgatggt tcagttatgg tggtcagtct ggttatgttg
       61 gccagtatta tcgctactca ttgttggccg tgggtgcggt gggcttggcg cgataacaac
      121 agggaaagat agtcagggga aactcccgcc cgccgtttac tggctgacat tgccctggcg
      181 catcgggatg tggctatcta tgcgttggtt ttgtcgtcgc ctggagccgg tgagcaaaat
      241 gactgctggt gtttatttaa gggcgtttcc acgacatatt ccggcacaga atgcggttct
      301 ggacgtcacc tttgaattcc ctcgtggacg agcgacaaaa gatcgactct atttctgtgt
      361 accgatgttg gatctggtgg ttccggaaga gggggagctc cgacaggccg tggcgatgct
      421 ggaaacatta cgcgaagagc aaggcagcgt tctggtccat tgcgcattgg gattatcgcg
      481 cagtgcgctg gtagtggcgg catggctgtt atgttacgga cactgtaaaa cagttgatga
      541 agcgattagc tttattcgag ccagacgctc gcatattgtg cttaaggaag agcacaaagc
      601 gatgttgaaa ttatgggaaa acaggtaagt ggattgagat gtggactgaa tatctacagt
      661 ccacatcaag accgtgtccg gttatgcagc aacaatgctg tcgatggctg cttttgcgtc
      721 agactgtgct ttcgctgcca tttccggacc gtatgcgatc ccttcggcga agacaaattt
      781 cacatcggta atgccgataa agccgaggaa cgtggacaga tacggcgtca ccaggtccgt
      841 tggtccatct ttgtggatcc caccgcggct ggtaataacg atggcttttt tacccgttac
      901 cagaccttcc ggaccgttct cggtatagcg gaaagtaacg cctgcgcgtg caaccaggtc
      961 aaaatagttt ttcaactgag ttgagatgtt gaagttatac attggtgccg caataacgat
     1021 aacgtcgtgg gctttcagct cggcaatcag ctcatcggaa agcgccagag cttcctgctg
     1081 acgcggagtc agcggcgcat cgctcggacg cagagcgcca accagttcgc catccagtac
     1141 cggaatcgga tttgcagcta ggtcgcgaac ggtgatttca tcagcggagt gcttttcgcg
     1201 ccattgttca acaaaataat cggacaactg attagactga gagtaccctg ccaggatgct
     1261 ggatttaaga actaatacct tgctcatggt gtttccttat agatgtttga atggacgatg
     1321 ccccgttgct tgctgacact ttattcacaa tcctgccaca gagatagcgc aataaatcga
     1381 agtctatgtt cgaatttatt gaacaacgca tagaaagccg cgatgtggta ctctatatct
     1441 atcatttaaa agaaaattaa tcaggcagac tactgcccac taacgttatg acagaacaac
     1501 aaaaattgac ctttactgcc ttgcaacagc ggctggattc gctgatgctg cgcgacagac
     1561 tgagtttttc tcgccgtctg cattgtgtta agaaggttaa aaatcctgat gcacaacagg
     1621 ccattttcca ggagatggcg aaagagattg accaggcggc agggaaagtc ctgctgcgtg
     1681 aagcggcacg accggaaatc acttatcctg acaatttacc ggttagtcag aaaaaacagg
     1741 acatactcga agcgattcgt gatcaccagg tggtgatcgt cgccggggaa accggctccg
     1801 gtaaaacgac tcagctaccg aaaatctgta tggagctggg gcgcggtatt aaagggctga
     1861 tcggtcatac ccagccgcgt cgcctggcgg cacgaacggt ggccaaccgt attgcggaag
     1921 agctgaaaac ggagccgggc ggttgcatcg gttacaaagt gcgtttcagc gatcacgtaa
     1981 gtgataacac gatggtcaag ctgatgaccg acgggatcct gctggcggag atccagcaag
     2041 accgcctgct gatgcagtac gacaccatca ttattgacga agcgcacgaa cgcagcctga
     2101 atatcgattt tctgctcggc tatttgaaag agttgctgcc gcggcgtcct gacctaaaaa
     2161 tcattatcac ttccgcgact atcgacccgg aacgcttttc gcgccacttt aataatgcgc
     2221 cgattattga agtctccggt cggacctatc cggtggaagt gcgctatcgc ccgattgttg
     2281 aagaagccga tgacaccgag cgcgatcagt tgcaggcgat ttttgacgcc gtagacgaac
     2341 taagccagga aagccccggc gacattctga tctttatgag cggcgagcgg gaaattcgcg
     2401 ataccgccga tgcgctgaac aagctgaact tacgccatac cgaaatcttg ccgctttatg
     2461 cgcggctttc gaacagcgaa cagaaccgcg tgttccagtc gcacagcgga cggtgcattg
     2521 tgctggcgac caacgtcgcg gaaacctcgc tgaccgtacc ggggattaaa tacgtcatcg
     2581 accccggtac agcgcgtatc agccgctaca gctatcgcac caaagtgcag cgtttgccga
     2641 ttgaacccat ttcgcaggcg tctgccaatc agcgtaaagg ccgctgtggt cgtgtgtccg
     2701 aagggatctg tattcgtctc tattccgaag acgatttcct ctcgcgcccg gagtttaccg
     2761 atccggagat tctgcgtacc aacctggcct cggttatttt gcagatgacc gcgctggggc
     2821 tgggcgatat cgctgcgttc ccgtttgtcg aagcaccgga taaacgcaat atccaggatg
     2881 gcgtgcgtct gctcgaagaa ctgggcgcga tcaccacgga tgaacaggcc agcgcctata
     2941 aactgacgcc gctcggtcgc cagctctcgc agttgcctgt cgacccacgt ctggcgcgta
     3001 tggtgctgga agcgcaaaaa catggctgcg tgcgtgaggc gatgattatc acgtccgcgc
     3061 tctccatcca ggatccgcgc gaacgtccga tggacaaaca gcaggcatcg gacgaaaaac
     3121 atcgtcgctt ccacgacaaa gaatccgact tcctcgcgtt tgtaaatctg tggaattatc
     3181 ttggcgagca gcaaaaggcg ctttcttcca acgccttccg tcgcctgtgt cgtaccgatt
     3241 acctcaacta tctgcgcgtg cgcgaatggc aggatatcta cacccagttg cgccaggtgg
     3301 tgaaagaact tggcattccg gtcaacagcg aaccggcgga gtatcgtgaa attcacatcg
     3361 ccttactgac cggtttgctt tcccatatcg gcatgaaaga tgccgataaa caggaatata
     3421 ccggtgcacg taacgcgcgt ttctccatct tccccggttc tggtttattc aaaaagccgc
     3481 cgaaatgggt aatggtggcg gaactggtag aaaccagccg cctgtggggg cgcattgctg
     3541 cgcgtatcga cccggaatgg gtagaacccg ttgctcagca tttgattaaa cgcacctaca
     3601 gcgaaccgca ctgggaacgg gcgcagggcg cggtgatggc aacggaaaaa gtcactgttt
     3661 atggtttgcc gattgttgct gcgcgcaagg tcaactacag ccagatcgat tcggcgttat
     3721 gtcgtgaact ctttattcgc cacgctctgg tggaaggtga ctggcagacg cgtcacgcat
     3781 tcttccgtga aaacctgaaa ctgcgggccg aagtggaaga gctggaacac aaatcacgtc
     3841 gccgcgatat tctggttgat gacgaaacgc tgtttgagtt ctacgaccag cgcatcagcc
     3901 acgatgtaat ctccgctcgt cacttcgata gctggtggaa aaaagtcagc cgcgaaacgc
     3961 ctgatttgct caactttgaa aagagcatgt tgatcaaaga aggggcggaa aaaatcagca
     4021 agctggatta cccgaacttc tggcatcagg gcaatctcaa gctgcgtttg agctatcagt
     4081 ttgagccagg cgcggacgcg gacggtgtga ccgtacatat tccgctgccg ttacttaacc
     4141 aggttgagga aagcgggttt gaatggcaga tccccggtct gcgccgcgaa ttggtgattg
     4201 ctctgattaa atcgttgccg aaaccggtac gccgtaattt tgtgcctgca ccaaactacg
     4261 ccgaagcgtt tttaggccgc gtcacaccgc tggagttacc gttgctcgac agccttgagc
     4321 gcgagttacg gcggatgacc ggcgttaccg ttgaccgcga agactggcac tgggatcagg
     4381 tgcccgatca cctgaaaatc accttccgcg tggtggatga caaaaacaag aagctaaaag
     4441 aagggcgctc gctacaagat ctgaaagatg cgctgaaagg caaagtgcag gaaacgctgt
     4501 ccgcggtggc ggatgacggt atcgagcaga gcggcttaca tatctggagt tttggtcagc
     4561 tgccggaaag ttacgaacag aagcgtggca actataaagt gaaggcgtgg ccggcgctgg
     4621 tggatgaacg cgacagcgtg gcgatcaaac tgtttgataa cccgctggag caaaaacagg
     4681 caatgtggaa tggtcttcgc cgtctactgc tgctaaatat ttcctcgccg atcaagtatt
     4741 tacatgagaa gttaccaaac aaagccaagc tggggctgta ctttaacccg tatggcaaag
     4801 tgctggagct gatcgacgac tgtatctcct gcggtgtgga tcaactgatc gacgccaatg
     4861 atggcccggt ctggacggaa gaaggctttg ctgcgctgca tgaaaaagtg cgcgccgaac
     4921 tgaatgacac ggtggtggat attgcgaagc aggtcgagca aatcctcact gcggtgttca
     4981 atatcaacaa acgtctgaaa gggcgggtgg atatgaccat ggcactgggg ctttctgaca
     5041 ttaaagcgca gatgggcggg ttggtatatc gcggttttgt cactggtaac ggcttcaaac
     5101 gtctgggcga cacgctgcga tatttgcagg cgattgaaaa acgactggaa aaactggcgg
     5161 ttgatccaca tcgcgatcgt gcacagatgc tgaaagtcga aaacgtccag caggcgtggc
     5221 agcaatggat caacaaactg ccgcctgcac gtcgtgagga tgaagacgtg aaagagatcc
     5281 gttggatgat agaagagctg cgcgttagtt acttcgctca acaacttggt acgccttatc
     5341 cgatttcaga taagcgtatt ttgcaggcga tggagcagat tagcggttaa ccctgctatt
     5401 tgcctaataa atctaaaacc cggtaagcat ttagcgccgg gttttttatt aattctaaaa
     5461 cggtaaggac aaaaattcag gaattcagaa aaatacaatt ctcagctgca agatgaataa
     5521 tgtttatcta cagcatttcc ttaa
//
LOCUS       DAJVQH010000186         5537 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJVQH010000186 DAJVQH010000000
VERSION     DAJVQH010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.188"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OQM87_003896"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OQM87_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCS1509833.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OQM87_003897"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OQM87_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCS1509834.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..>5537)
                     /gene="mdtF"
                     /locus_tag="OQM87_003898"
     CDS             complement(2659..>5537)
                     /gene="mdtF"
                     /locus_tag="OQM87_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HCS1509835.1"
                     /translation="STSDAAGNASITLTFETGTSPDIAQVQVQNKLQLAMPSLPEAVQ
                     QQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGSE
                     YAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQLGGMPQVADQQLNASIIVQTR
                     LQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAGA
                     NALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLVM
                     YLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVVV
                     ENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQFS
                     ITLISSMRLSVFVAMSLTPALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDST
                     RSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTTK
                     VLQQVTDYYLTKEKDNVQSVFTVGSFGFSGQGQNNGLAFISLKPWSERVGEENSVTAI
                     IQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLSL
                     AAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVALSDINQTISTAFGSSYVNDFL
                     NQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYNG
                     IPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYAI
                     SLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIGL
                     SAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHGA
                     GSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         1463 a         1288 c         1302 g         1484 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atatcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaaaaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg acttatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggctgt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag tccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccaactc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagctg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccac
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaag
//
LOCUS       DAJVQH010000187         5531 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJVQH010000187 DAJVQH010000000
VERSION     DAJVQH010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.189"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            100..1008
                     /locus_tag="OQM87_003899"
     CDS             100..1008
                     /locus_tag="OQM87_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCS1509836.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFY
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAADKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPETMIPGSTVPFISTK"
     gene            complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="OQM87_003900"
     CDS             complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="OQM87_003900"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1509837.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3323..4081
                     /gene="loiP"
                     /locus_tag="OQM87_003901"
                     /gene_synonym="yggG"
     CDS             3323..4081
                     /gene="loiP"
                     /locus_tag="OQM87_003901"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCS1509838.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4287..5207)
                     /gene="speB"
                     /locus_tag="OQM87_003902"
     CDS             complement(4287..5207)
                     /gene="speB"
                     /locus_tag="OQM87_003902"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCS1509839.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5343..>5531)
                     /locus_tag="OQM87_003903"
     CDS             complement(5343..>5531)
                     /locus_tag="OQM87_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509840.1"
                     /translation="FQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCS
                     GQAFPADNNGYAPPPCRK"
BASE COUNT         1325 a         1482 c         1478 g         1246 t
ORIGIN      
        1 ttcgctatac ccctttatag attctgaatt ctcacctatc ggctgttttc tcctcatctc
       61 atgtgacata ttcccatact ttactcaagg aagattgtca tgaaagcacc tgtttcatta
      121 acctcatttc gcccacaaaa atctcttgct atcgccattg gcgtgcttgc cgtggttgtg
      181 ctgccatttc tctcgtatta caccgttaat gaaggtgaac gcggcatttt gctgcgctac
      241 ggaaaaatcg taaaagttgc cgagccgggg ctgggtttta agatcccttt tatggaaagc
      301 gtggagaaaa tttccacgcg taatcaggct gtggtctatc agggattaca ggcctacagc
      361 cgcgaccagc aaccggcaca aatgacagta tcagtcagct tctacattaa accttcagaa
      421 gcaggcgcgg tttataccac ctacaacact attgaagcat taaaagatcg tctaattgtc
      481 cgtcagttgc caactcagct ggagaacatt ttcggccaat ataccgccat cagcgccgta
      541 caggatcgta caaaattggt tcaggatctg caaaatgcca tgcgcaaagc cgttgttggg
      601 ccagtggtaa ttgacggggt ccagattgaa aatattgatt tttctgacgc ttatgaaaag
      661 tcgattgaaa accgaatgaa agcagaagtc gccattgcca cgcgtaagca aaatctggaa
      721 acagagaaaa tccaggcgca aatagcggta actcaggccc aggcagaagc agacagtaaa
      781 ctggcggcag acaaagcaga agcggaaact attcgcgtaa gaggcgcggc agaggctgaa
      841 actattcgtt taaaaagtgc ggctgaagca gaagcaatcc gcctgcgcgg tgaagctctg
      901 cgggataatc ccggacttgt agccctcacc actgccgaac gttgggacgg taaactgccc
      961 gaaaccatga tccccggcag cacagttccg tttatctcta ctaagtaaaa aaagggccgc
     1021 tttcgcggcc cttctcacaa aacgtcttac tgattacagc agcgcttttg ctttcgcaac
     1081 aacgttatca acagtgaagc cgaactcttc aaacagcagc tctgccggag cagattcacc
     1141 gaaggtggtc atgccgacga tagcgccgtt caggccaaca tacttgtacc agtagtcagc
     1201 aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta
     1261 agccgcatcc tgcttgtcaa atgcgtcggt agacggcatg gacaccacgc gcgctttcac
     1321 gccttcggca gtcagttttt cgtaggcagc aaccgccagt tcaacttcag aaccggtggc
     1381 gatgaagatc agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat
     1441 gtttgccagc tgctcttcag tacgttcctg ctgcgccagg ttctgacggg agaggataag
     1501 cgcagtcggg ccgtcctgac gctcaacgcc gtatttccac gcgaccgcgg attcaacctg
     1561 gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctctac
     1621 cggctggtga gtcgggccat cttcgcccag accgatggag tcgtgggtgt aaaccatcac
     1681 ctggcgctgt ttcatcagcg cagccatacg tacggcgtta cgtgcgtatt ccacgaacat
     1741 caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc
     1801 ggtcataccg aactcgcgaa caccgtagtg gatgtagtta cctgcagcat cttcgttgat
     1861 tgctttagaa ccagaccaca gggtcaggtt agacggtgcc aggtcagcag agccgccgag
     1921 gaattcaggc aacagcgggc cgaacgcttc gatagcattc tgcgacgctt tacggctggc
     1981 gattttcgcc ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc
     2041 agacggcatt tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt
     2101 cgcgtaagcc gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc
     2161 atcccactga gcatagattt cagacgggat ttcgaacggt gcatatttcc agcccagctg
     2221 ttcgcgggtc agggcaattt cagcgtcgcc cagcggcgca ccgtgggagt cgtgagtacc
     2281 ggctttgttc ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc
     2341 agtcactgcg cgcgcttctt caactgcgcg tttgatggat gccgcgtcat gaccgtcgat
     2401 gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt gctgtgtcgt cggtgaacca
     2461 gccttcaacg tgaccatcga tagaaatacc gttgtcatcg tagaacgcaa tcagtttacc
     2521 cagcttcagc gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc
     2581 gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg
     2641 cgccgccagc gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag
     2701 cggaccggtg gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt
     2761 agagtgcagc tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg
     2821 cagcaggctg tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc
     2881 agcccaggac ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat
     2941 gtcagccata cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat
     3001 gctcagcgca cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg
     3061 gatgatgaag ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa
     3121 atttgccaac aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt
     3181 cagaaatctc tggatcatgc tcgcatgttg cgcaatctgc tcgcccgtcc gctgcgcttt
     3241 tccttatact gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct
     3301 gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc
     3361 aacggtactg accggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga
     3421 agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg
     3481 tcaggagatg gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct
     3541 gacaactatt gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt
     3601 gtatatggcg aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag
     3661 cgggctgatg gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg
     3721 gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt
     3781 gcgagtagct gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg
     3841 tgatctgggc gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga
     3901 tgattattct tacgatcttc tgcaccaacg cggcatcagc ccggcaggtc ttgccaccag
     3961 ctttgaaaaa ctggcgaagc tggaagaagg ccgtcaaagc tcaatgtttg acgaccatcc
     4021 tgcatccgcc gaacgtgccc agcatattcg cgatcgcatc agcgcggatg ggattaagta
     4081 aagcctggtg gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacattaatg
     4141 gcacatttgt taccttgtgc gcattgccgg atgcgatgct ggcgcatctt atccggccta
     4201 cgggtgccga acgtaggtcg gataaggcgt tcacgccgca tccgacatta atggcacgtt
     4261 ttacccgtgc gcatcgcatc tggtgcttac tcgccctttt tcgccgcctg aatatacagc
     4321 atctccagcg ccagcgtcgc cgcagccaga gcggtgattt ccgactgatc gtatgccgga
     4381 gccacttcca ctacgtccat cccaacgatg ttgagatctt tcaggccgcg taccagttta
     4441 atggcgcgat cggaggtcag gccgccaatc actggcgtac cggttcccgg cgcgaaagca
     4501 ggatccaggc agtcgatatc aaaagtcagg taaaccggca tatcacccac aatctgtttc
     4561 acctgggcga taacgtcatc cacgctgcga tcgttcacct ggcaggcgtc cagcacggta
     4621 aagccgttgt ctttatcaaa ctcggtacga ataccaatct gcacggaatg attcgggtcg
     4681 atcagacctt ctttcggcgc ggtatagaac atagtgccgt ggtcaaattc acaaccgttc
     4741 gcataggtat cggtgtgggc gtcaaagtgt accagcgcca ttttgccgaa atgcttcgca
     4801 tgagcacgca gcagcggcag cgtaacaaag tggtcaccac cgaaggagag catacgctta
     4861 ccggcagcca gcagcttctc ggcgtgcgcc tgcagctttt cgctcatctc acgggcatcg
     4921 ccaaaggcat ataccagatc gccgcagtcc acgacgttca gacgctcacg catgtcgaaa
     4981 ttccacggga agcggttgtg ttcccaggcc agattcgtcg aaacctgacg gatcgctgcc
     5041 ggaccgtggc gaccacccgc acgaccagaa gtggccatat cgaacggcac gccagtaatc
     5101 acccagtctg catcgctgtc atacggctgg aagttcatcg gcaggcgtaa aaaaccaaag
     5161 gcattggaaa ccagtgagtt atcgtattga tgacctaagg tgctcatgga tggactccta
     5221 ttacaaagtc gatataaaaa aacccttccg cgtcgttagg cccgacgagg aagggttgga
     5281 tttgcgaaac acaagtttgt gctaattatc gccttaaatg ggcgaggcat caagcgaggt
     5341 tgctatttcc gacacggtgg cggcgcataa ccattgttat ctgccggaaa ggcttgtcca
     5401 gagcacggcg gcagcgcgtt ttgcgccggg ccttgtaatt caaaggcggc aaagccgatt
     5461 accccggtat tacgcacatc gccttgcgcc gaattggctg cgtaggaatc agcaacgttg
     5521 gcgaactgaa a
//
LOCUS       DAJVQH010000188         5415 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJVQH010000188 DAJVQH010000000
VERSION     DAJVQH010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.190"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..87)
                     /locus_tag="OQM87_003904"
     CDS             complement(<1..87)
                     /locus_tag="OQM87_003904"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCS1509841.1"
                     /translation="MALESDERMLPPNIRGYAPQITGIAETNA"
     gene            complement(249..3557)
                     /gene="icsA"
                     /locus_tag="OQM87_003905"
                     /gene_synonym="virG"
     CDS             complement(249..3557)
                     /gene="icsA"
                     /locus_tag="OQM87_003905"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCS1509842.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGSS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4086..5288
                     /gene="virA"
                     /locus_tag="OQM87_003906"
     CDS             4086..5288
                     /gene="virA"
                     /locus_tag="OQM87_003906"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCS1509843.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1682 a         1217 c          896 g         1620 t
ORIGIN      
        1 tgcattagtt tctgcaatac cagtaatctg aggcgcataa ccacgaatat ttggcggtag
       61 cattcgttca tcgctttcta atgcaattcc tgtgtaacgc caagcaacag cgcattctgg
      121 cgcatgatct ggtaaacacg tttggcattg atcgcaggca taccatcatg tgcacaaaaa
      181 cccaccgagc ttttacacgg tgggtcccag agaaatgcag gacatcaaca cgccctgcat
      241 ttttattatc agaaggtata tttcacaccc aaaatacctt gggtatctct gtaactgtta
      301 ttacccaatt gctgagatac gtttccccag atgcttaggt tatctgcaag acgccccccc
      361 acaccagttt tgagttcaat cacattacgg ttgcctatct ggtgatttga catgccattc
      421 ataattacac catattgctt tgagctccat ttccaattca cttccaaaaa tggttcgaac
      481 ttacgaagag tctccttatc gatacaactc tgactaatta aggatgtacg caatcccaat
      541 tttgcttgaa tattattgac cccagaggat gtgaccatag tgccattgtg ttcaatatag
      601 tcgtttgctt ttaccccatg cctagttatc tggacttgtg gattgagata caatgcgttg
      661 tcaatattat tatgagcagt ccagcgtatg gttgggtaga tatagccagc ttccaaagct
      721 cctgttattc ctttgctgga atatttctca ccagttaacc catctccttt gactgtatta
      781 ttaaaccaac tgtactgcaa ccaagcatcc atatatagac ctgttcgttc tttttcatcc
      841 tgaaaccaag aactgtacaa accggcagta taaccgctca cagttcctcg tgaactataa
      901 ctagtatact tattatgagt tttgctatcc tgatatccat aagctcccat aatacccaca
      961 tgcaagccat catgatcagt gaagtttgtc ttcaaaatat ctccaccaat ctgataaacc
     1021 atcctgttag ctgtggtttt taattgtcta tcagctaaat tatgctcatt atgtccacca
     1081 acagtacgaa tccacatggt ggtttcgctg agctgacctg tagtaggatc aacaatagtg
     1141 ctaccgtgtc ggtcatagag agaaggacta aacatcgtgt tcattgctat caggttagca
     1201 atataactgc cagcttcagg gcgccatgtg tgaatactac cttcacccaa attaagtgaa
     1261 gatacaagct gtgaactagc tcggcgacta ctcatttgag tagactcttg attgctcatc
     1321 tgttttgatt cttgattatc catctgacta gttagatacc acttattggt attcagtcca
     1381 gatacagtgc cctgtttcag gcgatagtca taactaccag caacaatacg gcctttctga
     1441 ataaaagcat tcttatcaga agaatctgtc gaaataattt gaacaccttc aagagtttta
     1501 tctccagagc ctccctcatt tgtcactgca acataggtaa taccagacgc atggcctgca
     1561 atttccagcc ggtcagtttt actgttgtca ttgccgagaa ccgcgcccaa agataatatt
     1621 ccgccctttc catgccaatt acctttctga acataggtct gaccgacatt tgacgaacta
     1681 ttattgagaa taacatgacc gtttttctcc agagtcatgt tacctgtgac aatgacgggg
     1741 tcaggcaaaa aaggggcatt aatattattg attagcacag tcccactatt taataaagtg
     1801 ttaacagttt ggttcatgcc tgaaaaattc aaggtagcac ctttattaac aataacacca
     1861 gcggtacgtg tcatagcttc aactgtcccc atttttagaa tgcctgcatt gatgttggtg
     1921 aaaccagagt aggtattttt ttccgccaaa atgagagttc cctcccctga ttttgttagg
     1981 gattttccgt cccagctatt tatattatga tggctgagat tatctgccag aatagtacta
     2041 acagtaagtt cagcgttttc tttcagattg aactctccat gactgtcgcc atctgtatca
     2101 ttccagctca aaccgtagat caatttattc tggtcactga tatagctttt tgtatagtca
     2161 atagcaccag tagtgagtaa cggattgatg atagatatat tagaaacact ggaacctgtt
     2221 atccctttat ctgcacttag cagtttatag tccttctgcg cccagttatt tataatagct
     2281 tccactttta cattgttgcc aaatgacata tttccagaag cttccagact actgattcca
     2341 gctaaattta aagaactgtt ctcaccaaaa gaaacgtcac ctgaaacagt gaatttgtta
     2401 cctgatagtg aaacagttgt acaatcacca gcacataaat caccttcaat agttggtgtg
     2461 gtcccagtgt aatcattgtt gataattttc agaatggatg aattgttaac ctgtacatca
     2521 ccagtaatga cagaaccttc atggagtatt aatgaattgt ttccaccagt gatgtggata
     2581 gcattacctg ctacattttg tgttccatca tcttctttac ctgaaattat atatccgcta
     2641 ttagcaatgg tcatattgct accatttata gcatcccccc aatgtccacc attaccgcct
     2701 aaaatagctc cattgttgat tacagacagg ttatctcctg tgatagcatt accaccgtag
     2761 ccatcataac cataaccgta agcagcacct ccgttgcctc cagcgaacgt gcccttattg
     2821 attacagaga ggctgcttcc tgtgatggca tcacctccat taccgccatc accctcacca
     2881 taattgttac ctccattacc accggaaata gtccctttgt tgattatggg tagattgact
     2941 cctgtgacgg catcacctcc attcccccca tcactatcac tataactatc gccaccatgt
     3001 ccacctgaaa taatttctcc atttattata aacagattgt ctcctgtaac agcctcacca
     3061 ccatcaccgt tatggtcagc accgctaccg ccgctaccac caagaatcat gccttgattg
     3121 attatagaca agtcagatcc ggtaatagag tcaccaccat taccgccaca actattacca
     3181 tcattattat caccagcacc accatgacct gcgctaatca taatatcaga gttaatccgt
     3241 agcttagttc tagatgcatg agaggggttg gaagaagtta tataccacgc atccattcca
     3301 tcttcaccag ctcccaaggg agaaagtcca tcaacaggtg ttaataattt ttcataattg
     3361 tcctctgaaa aatgaagttc ttgagtaccc gaaagaggag tagcaaaagc tattggcccc
     3421 ccgagcaaca gggatgcacc aacaacaaaa ggagaaaaag acaatttgca tgttttttcc
     3481 tttaccgtag gtaattctcc ggcccccccc tgtgaacatt gggtcatatt acaaaaaaat
     3541 ttgtgaattt gattcatgca ctatattatc agtaagtggt tgataaaccc ctgaaaagat
     3601 tccgttttat ccggaataaa gggacgatat atgcaaaaac atattaaaca aagcctcaac
     3661 caaaacatga gagctgtgtt atatatcaat gcaacgggag aaatttacct gtgtcttgct
     3721 ttgaaaaaaa gaggggaaaa gctatatgtt cgataataaa ttaataattt tactatgata
     3781 gtctgcttca gaccgcaact tttcagtcct ttctgtacgc ttgcccaaag tttaaccact
     3841 ttgtttttct atacacccaa tggttctatc tgccccttga tgttaatccc caaagccaat
     3901 aacatgaatg tattcatatc acaactttcc taccagactc tccaatgcaa taactgcata
     3961 attgttttta gtggaaatgt atcgttttct taaagagaag aataacattc cattttatta
     4021 tgttcccaat ttaattatat taatatatat tggcattaat aggaaaatac atcaggagaa
     4081 atcaaatgca gacatcaaac ataactaacc atgaaagaaa tgactcatca tggatgtcaa
     4141 ctgtcaaatc aactacagaa gtaagttgga ataaattgag tttttgtgac gttttactga
     4201 aaataataac tttcggcata tattctccac acgaaactct agccgaaaaa tatagcgaaa
     4261 aaaaactaat ggatagtttc tcaccatcac tatcacaaga taaaatggac ggagagtttg
     4321 cccacgcaaa catagatggc atttcaatac ggctatgcct taacaaaggc atatgttcgg
     4381 ttttttatct tgatggagat aaaatacaat ccactcaact ttcatcaaaa gaatataata
     4441 acttgttaag ttcactcccc cccaaacaat tcaacttagg aaaagtacat actattacag
     4501 ctccggtatc agggaatttt aaaactcaca agcctgcacc agaagttatt gaaacagcca
     4561 ttaattgctg tacatcgata ataccaaacg atgattactt ccacgttaaa gacactgatt
     4621 ttaatagtgt ttggcatgat atttacagag atattcgagc atctgacagc aatagtacaa
     4681 aaatatattt caacaatatt gaaataccac taaaattaat tgcagatcta attaatgagt
     4741 taggaattaa tgaattcatt gatagcaaaa aagagcttca aatgctaagt tacaaccaag
     4801 taaataaaat aatcaattca aacttccccc aacaagacct atgttttcaa acagaaaaac
     4861 ttttgtttac ttcactcttt caagaccctg catttatatc tgcactaaca tctgcattct
     4921 ggcaatctct tcacatcacg tcttcctctg tggaacacat atatgcccaa attatgtccg
     4981 aaaacataga aaacaggctt aatttcatgc ctgaacaacg agttattaac aattgtggac
     5041 atattataaa aatcaacgca gttgtcccta aaaacgacac tgcaatctct gcctctgggg
     5101 ggagggctta cgaagttagc tcatcaatat taccatcgca cataacatgt aatggtgttg
     5161 gtattaataa aatagaaaca agctatctcg ttcatgctgg aacacttcca agcagtgaag
     5221 ggcttcgcaa tgctatccct ccagaaagtc gtcaagtatc atttgccatc atatctcctg
     5281 atgtttaagc caatattata ttggctgcca gactgtaaaa aaacgttctt cagaacagct
     5341 ctataaacta ttaaaccaaa gctaaaagga tggggaccaa tagaatctca gcccatcctt
     5401 tttatatcat tatga
//
LOCUS       DAJVQH010000189         5381 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJVQH010000189 DAJVQH010000000
VERSION     DAJVQH010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.191"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..233
                     /locus_tag="OQM87_003907"
     CDS             <1..233
                     /locus_tag="OQM87_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1509844.1"
                     /translation="MSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYI
                     EMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
     gene            234..491
                     /locus_tag="OQM87_003908"
     CDS             234..491
                     /locus_tag="OQM87_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509845.1"
                     /translation="MIRGDSLWAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLG
                     STAGKWLGALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            531..1071
                     /locus_tag="OQM87_003909"
                     /pseudo
     CDS             531..1071
                     /locus_tag="OQM87_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            1138..2556
                     /gene="dcm"
                     /locus_tag="OQM87_003910"
     CDS             1138..2556
                     /gene="dcm"
                     /locus_tag="OQM87_003910"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCS1509846.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2537..3007
                     /gene="vsr"
                     /locus_tag="OQM87_003911"
     CDS             2537..3007
                     /gene="vsr"
                     /locus_tag="OQM87_003911"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCS1509847.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2996..3916)
                     /gene="yedA"
                     /locus_tag="OQM87_003912"
     CDS             complement(2996..3916)
                     /gene="yedA"
                     /locus_tag="OQM87_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCS1509848.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4089..5006
                     /locus_tag="OQM87_003913"
     CDS             4089..5006
                     /locus_tag="OQM87_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCS1509849.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGTIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5085..5267
                     /locus_tag="OQM87_003914"
     CDS             5085..5267
                     /locus_tag="OQM87_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCS1509850.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT         1330 a         1274 c         1527 g         1250 t
ORIGIN      
        1 gcatgagccg tcgcggtaac tgccatgata atgcggttgc agaaagtttt ttccagttgt
       61 tgaaacgtga acggataaag aaaaagatct acggaacgcg ggaagaagcc cgcagtgata
      121 tttttgatta catcgaaatg ttttataaca gtaagcgtcg gcatggttct agcgaacaga
      181 tgtcaccgac agaatatgaa aaccagtatt atcaacggct cggaagtgtc tagattatcc
      241 gtggcgattc attgtgggct ttctatacat ggtgtggcta tttcgtctgg gcaatggcgc
      301 gatacatctg ggtgatgagc cggatccccg atgcccccgt gtcgggattt gaaagtgatt
      361 taggatctac cgcggggaag tggttaggag cattggtcgg atttctgttt atggccctgg
      421 ttggtgcctt gttaggcggc attgcatggt acacccgacc tcgtcccgca cgttgcagac
      481 gctatgaata aaaactgcta aggttgcgac tctttggcaa cggagcgcgt atggagttac
      541 aacactggca ggcacagttc gagaactggt tgaaaaacca tcatcaacat caggacgcgg
      601 cgcatgacct gtgtcatttt cgccgtgtct gggctacggc acagaaactg gcggcagatg
      661 acgatgtcga tatgctggtg attttaaccg cctgttattt tcacgatatc gtgagcctag
      721 cgaaaaatca tccgcaacgg cagcgttctt caatcctggc ggcagaagag acgcgtcgcc
      781 tgctccgtga agagttcgtg caatttccgg cggagaaaat tgaggccgtt tgtcatgcca
      841 ttgccgctca cagtttcagc gcgcaaatcg cccccttaac aacggaagct aaaatcgtcc
      901 aggatgcgga tcggctggaa gccctggggg cgattggcct ggcgcgcgta tttgccgttt
      961 caggggcatt gagcttggcg ctgtttgatg gcgaagctcc gtgccgaact ggcgggggag
     1021 aatgaaggta tcgatcacaa ggtgatagat gcgttttcac ccgctggctg agcgcgtggc
     1081 cctaaacggc agtaattatg ttaacctgtc ggccatctca gatggccggt taaatctatg
     1141 caggaaaata tatcagtaac cgattcatac agcaccggga atgccgcaca ggcaatgctg
     1201 gagaaactgc tgcaaattta tgatgttaaa atgctggtgg cgcagcttaa tggtgtgggt
     1261 gagaatcact ggagcgcggc aattttaaaa cgtgcgctgg cgaatgactc ggcatggcac
     1321 cgtttaagtg agaaagagtt tgcccatctg caaacgttgt tacccaaacc accggaacat
     1381 catccgcatt atgcgtttcg ctttatcgat ctatttgccg gaattggcgg catccgtcgt
     1441 ggttttgaat cgattggtgg acagtgcgtg tttaccagcg aatggaacaa acatgcggta
     1501 cgcacttata aagccaacca ttattgcgac ccggcgacgc atcattttaa tgaagatatc
     1561 cgcgacatca ccctcagtca tcaagaaggc gtgagtgatg aggcggcggc ggaacatatt
     1621 cgtcaacaca ttcctgaaca cgatgtttta ctggccggtt tcccttgtca gccattttcg
     1681 ctggctggcg tatcgaaaaa gaactcgctc gggcgggcgc acggttttgc ctgcgatacc
     1741 cagggcacgc tgttctttga tgtggtgcgc attatcgacg cgcgtcgtcc ggcgatgttt
     1801 gtgctcgaaa acgtcaaaaa cctgaagagt cacgataagg gaaaaacgtt ccgcatcatc
     1861 atgcagacgc tggacgaact gggctatgac gttgccgatg cagaagataa cgggccggac
     1921 gatccgaaaa tcatcgatgg taaacatttc ctgccgcagc accgtgaacg catcgtgctg
     1981 gtgggttttc gtcgcgatct gaatctgaaa gccgatttta ctctgcgtga tatcagcgaa
     2041 tgtttccctg cacagcgagt gacgctggcg cagctgctgg acccgatggt tgaggcgaaa
     2101 tatatcctga cgccggtgct gtggaagtac ctctatcgat atgcgaaaaa acatcaggcg
     2161 cgcggtaacg gcttcggtta tggaatggtt tatccgaaca atccgcaaag cgtcacccgt
     2221 acgctgtctg ctcgttatta caaagatggc gcggaaattt taatcgatcg cggttgggat
     2281 atggccacgg gtgagaaaga ctttgacgat ccgctgaatc agcaacatcg tccacgtcgg
     2341 ttaacgcctc gtgaatgcgc gcgcttaatg ggttttgaag cgccgggaga agcgaaattc
     2401 cgcattccgg tttcggacac tcaggcctat cgccagttcg gcaactcggt ggtcgtgccg
     2461 gtctttgccg cggtggcaaa actgcttgag ccaaaaatca aacaggcggt ggcgttgcgt
     2521 cagcaagagg cacaacatgg tcgacgttca cgataaggcc acccgcagca aaaatatgcg
     2581 cgcgattgcc acgcgtgata cggcgattga gaagcgcctc gccagtttgt taaccgggca
     2641 gggccttgcg tttcgcgttc aggacgccag tctgcccgga cgcccggatt ttgtcgttga
     2701 tgaatatcgc tgcgtgatat ttacccacgg ctgcttctgg catcatcatc actgctatct
     2761 gtttaaagtg cctgcgactc gaactgagtt ctggctggag aagataggta aaaatgttga
     2821 acgcgatcgc cgcgatatca gtcgcttgca ggagctcggc tggcgcgtac tgattgtctg
     2881 ggagtgtgcg ttacgtaggc gcgagaagct gacggatgcg gcgcttaccg agcgtctgga
     2941 agagtggatc tgcggcgaag gtgccagcgc gcagatcgac acgcagggga ttcatttact
     3001 cgcttgatgc atcctgaata actggcgcga caacgggttt ggccgggaag agatattttc
     3061 ccaacgtgac cagtaccacc gcgaaaacaa ttacgccgag cgccagccat tcaatcttcg
     3121 acagcgtttc gccacccagt cccgtaccca gcaagaccgc gaccaccggg ttaacgtagg
     3181 cgtagctggt ggcgagagcc ggactgacat tacggattaa atacatataa gcgttaatgg
     3241 cgataatcga accaaacagc gccagatagc cgaccgcaag gaagcctgac agggaaggga
     3301 gcgccgtcag tttttcaccc gcaatcatcg atgcgatcat taacaccacg cctgccgcca
     3361 gcatctcaat cgcacccgcc atcatcccta caggtaaggt aatgcgcgag ccataaactg
     3421 agccaaacgc ccagctaatc gagccgatta aaatcagaat cgcgccccac ggattgccgc
     3481 ttaaatttcc accgctattg agcatgatga ttccggcaag cccaatggca atacccaccc
     3541 attccagttt gcgcgtttta atgccaaaca ggcgactgaa gcacagggta aagaggggca
     3601 cggttgcaat cactacggcg gcgatgccgg aaggaacatt ttgatgttcg gcaaccgtca
     3661 ccatgccatt accgacagcc agcaataaca ggccaatcag cgcggcattg agcagcggac
     3721 gtagcggggg aagtttgtgt ccgcgcagta gcaaaaatgc cagcaataaa ataccggctg
     3781 ccaggaatcg aacgcccgcc atcattaacg gaggccagct tcccacgcca atccgaatga
     3841 caaaataggt tgagccccaa atgatataca acgcaaacag cgcgccaaaa agcggtaaca
     3901 actggcggaa acgcataatc cctcacggcg gaaataaaaa ggtggttcat agtaaacgtg
     3961 aaaatcattc tgctggctag agatataatt gcacttgatt gttaaataaa tgttgacctg
     4021 tgaagcaagt atcagagctg ccgtttttgc ttcatactta cacccttcag caataaaaat
     4081 gagagggaat gcttttggcc gggagtagtt tactgacgtt gctcgatgat atcgccacac
     4141 tgctggacga tatctctgtg atgggcaaac tggcggcgaa gaaaaccgcc ggtgtattag
     4201 gggatgattt atcgctcaat gcgcaacaag tttcaggcgt gcgggccaac cgggaacttc
     4261 ccgtggtctg gggcgtggcg aaaggatcgc tgatcaataa agtgattctg gtgccgctgg
     4321 cgctgatcat cagtgcgttt atcccgtggg cgattacgcc actgttgatg attggtggcg
     4381 cgtttctctg ctttgaaggg gtagagaaag tgctgcatat gctggaggcg cgtaaacata
     4441 aagaagatcc agcgcagagc cagcagcgtc tggagaagct ggcggcgcag gatccgctga
     4501 agtttgaaaa ggacaaaatt aaagggacga ttcgtaccga ttttattttg tctgcggaaa
     4561 tcgtcgccat cacgctgggg attgtggcag aggcgccgtt gcttaatcag gtgctggtgc
     4621 tttcaggcat cgcgctggta gtgaccgtgg gcgtctacgg tctggtaggg gttatcgtta
     4681 agattgatga cctagggtat tggctggcgg aaaaatccag cgcgctgatg caggcattag
     4741 gtaaagggtt attgattatc gcgccttggc tgatgaaagc attatcgatt gtcggcacgc
     4801 tggcgatgtt cctcgtcggc ggcgggattg tggtacatgg tattgcgccg ctgcatcacg
     4861 ctattgaaca tatcgccggg cagcaaagtg cagtggtggc gatgatatta ccgactgttt
     4921 taaatctgat tcttggattt atcatcggcg gcatcgtggt gctgggagtg aaagcagtag
     4981 cgaaaatgcg cggtcaggca cattaatact cgcactggct attatgcaaa attcgccatc
     5041 ttcaactaag gtgaacaagt ttcactcgaa aaaaggaggc gagtatgagc tttatggtta
     5101 gtgaggaagt tacggttaaa gagggcggcc cgcgaatgat tgtcactgga tactccagcg
     5161 gtatggttga gtgtcgttgg tatgacggtt acggggtcaa gcgggaagct tttcatgaaa
     5221 ccgatcttgt tccgggggag gggagtcgtt ctgcggaaga agtttgaaag ggtatcatta
     5281 acaccaggcc ggatcacatc cggcctggtg tcccaatgcc tgcaatgaat gatagtcgcg
     5341 tcttatcatg ccaccattat aaaattatcg ctgttttgcg g
//
LOCUS       DAJVQH010000190         5368 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJVQH010000190 DAJVQH010000000
VERSION     DAJVQH010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.192"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..89)
                     /locus_tag="OQM87_003915"
     CDS             complement(<1..89)
                     /locus_tag="OQM87_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1509851.1"
                     /translation="MLPNRILFSLMLIVSSGVVASKKAHELYD"
     gene            complement(100..333)
                     /locus_tag="OQM87_003916"
     CDS             complement(100..333)
                     /locus_tag="OQM87_003916"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509852.1"
                     /translation="MSKEYTLSVPPFSLNTLGVDMYNNSSYIRKSYIDIPMYNRNLCD
                     FHDLTYSIKSATAILNGKQRDLLKEEHIQVQDK"
     gene            complement(330..1601)
                     /locus_tag="OQM87_003917"
     CDS             complement(330..1601)
                     /locus_tag="OQM87_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1509853.1"
                     /translation="MWSGKVILLSLGLSLASFCHASTDIDQLYERIVNKDQTALKELK
                     KLADTNNTQALVLLGFIYEHGVTVSVDIPKAIQWYEKACNQGGEYGCYNARYFFQYGK
                     GVTLDVTRAQKYASKSKTDDLNIPPDLMNKIINQAYELKSGAETDKTKRASLIEFVSR
                     YVGQAPENDLLFWGRIGFSKPEILRLATLWAQEGDPEMDYIVGNLYLDDFSPSDDRRS
                     LDTIKKALQWFRTAAEKGYPMAQYTMGDTYENGSAGLKIDAVEAKKWYELAANKNDEK
                     ALVALGNIYYSGLTGEVDYSKASMLFDKAEQQGAKKAALWLSWMYYNGLGETLDCDKV
                     WDYYEKGAGKYSMKFNKDEYLTRCNQDQKTRETQGDTLPYITLKHYGTYGFNANEKPK
                     ICDVSIEINADKISSITNLQLTLELKGGGRE"
     gene            1995..3365
                     /locus_tag="OQM87_003918"
     CDS             1995..3365
                     /locus_tag="OQM87_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1509854.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFIVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
     gene            3547..5368
                     /locus_tag="OQM87_003919"
                     /pseudo
     CDS             3547..5368
                     /locus_tag="OQM87_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
BASE COUNT         1658 a         1120 c         1035 g         1555 t
ORIGIN      
        1 ctgtcataca attcatgcgc tttcttactt gccaccacgc ctgatgaaac tatcagcatt
       61 aaagaaaata atattctatt aggcaacata aaatatctct tatttatctt gtacctgaat
      121 atgctcttct tttaataaat cacgttgttt tccattcagt atcgctgtcg cagatttaat
      181 agaataagtc aaatcatgaa aatcgcataa attacgatta tacattggga tgtctatata
      241 gctttttctt atataactgg aattattata catatccaca ccgagcgtat tcaaactaaa
      301 aggagggaca gaaagagtat attctttact cactccctcc cccccccttt tagctcaagc
      361 gtcaactgca aattagttat actactaatt ttatctgcat taatttcaat actgacatcg
      421 catattttgg gtttttcatt tgcgttaaag ccataggtcc cgtaatgttt taacgtgatg
      481 taaggtaaag tatcaccctg tgtttctcgg gttttctgat cttgattaca acgggttaaa
      541 tattcatcct tattgaattt cattgaatat ttccccgcac ctttttcgta ataatcccat
      601 accttatcac aatcaagcgt ttcacctaat ccgttgtaat acatccaact taaccacaat
      661 gcggcttttt ttgccccttg ctgttcagcc ttgtcaaaaa gcatgctcgc tttagaataa
      721 tcaacctcac cggtcaaacc agaatagtat atattcccca gggccactaa tgctttttcg
      781 tcattcttat ttgcagctaa ttcgtaccat tttttggctt cgacagcatc aatctttaaa
      841 ccagcactgc cattttcgta ggtatcgccc atggtatact gcgccatcgg ataacctttt
      901 tctgccgcag tacgaaacca ttgcagtgct ttctttatgg tgtctaaaga acgtctatca
      961 tcagaaggtg aaaaatcatc gagataaaga tttccgacaa tataatccat ttcggggtcg
     1021 ccttcctgcg cccatagagt cgccagacgc agaatttcag gtttactgaa accaattcgc
     1081 ccccaaaaaa gcaaatcatt ctcgggtgcc tgaccgacat aacgactgac aaattcaatt
     1141 aaactcgccc gtttcgtttt gtctgtctca gccccggatt tcaattcata ggcctgattg
     1201 attattttat tcatcaaatc aggcggaata ttaagatcgt cagttttcga cttactggca
     1261 tacttctgtg ctcgtgtcac atctagggtg acgcctttac catattgaaa gaaataacgt
     1321 gcattataac aaccgtattc gcccccctgg ttacaggctt tctcatacca ctgaatcgcc
     1381 tttgggatgt caacagatac tgtcactccg tgttcataaa taaatcccag tagcactagc
     1441 gcctgcgtgt tattggtatc agctaatttt ttcagttctt ttaacgccgt ctgatcttta
     1501 ttgacaatac gttcatacag ttgatcaata tctgttgaag catgacaaaa agaagccaga
     1561 gataaaccca atgataataa aataacttta ccagaccaca tacattcctc cttgatatat
     1621 tcaaccacca aacatccacc tcatgaataa atacgctgcc ataacggccc aggtaataag
     1681 taaacattta agctgaaaca gcataaccag tgcctccaac gcttctattt attgcgataa
     1741 aataacacca tcctttgata aaaagtcaca cgcccatcaa taaatccaaa aataatgtca
     1801 ttagttgcag gataaaatca tttgagcttt tcatttgtta tatttaacgc atgatgtatt
     1861 aatggcaaga atatgagaga aactcatacc tgacgtaaaa atcactctct tatgtaatat
     1921 ttacttatta acactctgtt tacctaacat gcgctccgca ctacactatg tgggcaattt
     1981 tttaagaggt ggtaatgaac gattataaaa tgacgccagg tgagcggcgc gcgacctggg
     2041 gtttagggac cgtattttca ttgcgcatgc tgggcatgtt tattgtactg ccggttctga
     2101 ctacgtacgg tatggcactg caaggcgcca gcgaagcatt aatcggtatt gccattggta
     2161 tttatggtct gacccaggcc gtttttcaga ttccgtttgg cctgctttcc gaccgcattg
     2221 gtcgaaaacc attaattgtc ggtgggctgg cagtgtttgc cgccggtagc gttatcgctg
     2281 cgctttctga ctccatctgg ggaattattc tgggccgggc gctacaaggc tccggtgcga
     2341 ttgccgctgc cgttatggcg ctgctttccg atctcacgcg cgaacaaaac cgtaccaaag
     2401 caatggcgtt tatcggcgtg agctttggca ttacctttgc cattgcgatg gtgcttggcc
     2461 cgatcatcac tcacaaactt gggctgcacg cgctgttctg gatgatcgct attctggcaa
     2521 cgaccggcat tgcgttgacc atttgggttg tgcccaacag tagcactcac gtacttaatc
     2581 gtgagtccgg aatggtgaaa ggcagtttca gtaaagtgct ggcggaaccg cggctgctga
     2641 aactcaactt tggcattatg tgtctgcata ttttgctgat gtcgacgttt gttgccctgc
     2701 ccggacaact ggctgatgcg gggttcccgg cggctgaaca ctggaaggtc tatctggcga
     2761 caatgctaat cgcctttggc tcggtcgtgc ctttcattat ctacgctgaa gttaagcgca
     2821 aaatgaagca agtctttgtc ttctgcgtcg ggttgatcgt ggttgcggaa attgtgttgt
     2881 ggaacgcgca aacgcagttc tggcaactgg tggtcggcgt gcagcttttc tttgtggcgt
     2941 ttaatttgat ggaagccctg ctgccctcac ttatcagtaa agagtcgcca gcaggttaca
     3001 aaggtacggc gatgggtgtt tactccacca gccagtttct tggcgtggcg attggcggtt
     3061 cgctgggcgg ctggattgac ggcatgtttg acggtcagcg ggtatttctc gctggcgcaa
     3121 tgctggccgc agtgtggctg gcagtcgcca gtaccatgaa agaaccgccg tatgtcagca
     3181 gtttgcgcat tgaaatccca gaaaacgtaa tggcagatga aaaactggaa aaatgcttac
     3241 tgacaacgcc tggtgtcaaa gatgtactgc tggcgaagtt agaacattgc gtctatgtga
     3301 aagtagacag taaaatcacc aatcgttttg aaatagagcg agtaatcaac gaagtatctt
     3361 attaaataat aagcacggtg ctattcatga aaaataaaat agcaccgtac ttcgtaacac
     3421 cataatcctt taaaccctcc attattaata cttcgcttaa ttccatataa ctcgacgatt
     3481 acattagaag taaactatac tttttatgaa atatcaggga taaattattc acatggaaaa
     3541 tacatcatgt acaaatcatt atttactatt atcattctac tgtatagcgc tagttcatat
     3601 gcaaaaagcg aatcccctcc cgcaagcact ataactttca ttgcacaaat cgatcatgtt
     3661 gagctaaata aaacagcatt atctcatgat atgaaactcg ttcttgccga tcgtttttat
     3721 ttcaaaacca gtaatccttg cgaaacaaat tcaatttctg ctcgaccaga atcaatggat
     3781 attactgaca aagcctatgc caacaaatta aaaaaattag aaactccttt tcttgatgat
     3841 cgctatttct ttatgaccat caatcagtgt gacggtaatg gcactcccat gctgacagac
     3901 ataaaagtat gtacaacaga attatgtggt gctgaatata caaagaaaat gtctactgta
     3961 tggctcgatg aaactttaaa accaactgct aaacgtctgg caatttattt tattcaatct
     4021 cctttaccct atgacgagga gaaaaaacaa tggaaagtca ccggatggta tatctcatca
     4081 actgataatc agtcttcaac taaacagatt gcatttgaag gatatacaga cacagataac
     4141 ttctccgatt ctaaatttat ttcttcattt aaatcctggt ttgaaagtgg aaagataaaa
     4201 aacattgcta actataatcc aaaaggagaa agagatggaa aatacgaaag ttattacaat
     4261 agcaatggta acgtctatga atctctaagt ttttctaatg gtcttatcaa cggtgagttt
     4321 attacatatt atgaaaatgg ggccattgaa agtaaacgtc attttaataa cgacaaaatt
     4381 aaagatggtg aatgccctca tttttatgaa gatgggaaaa tcaaacagca acatagctat
     4441 ctgaatagta aactcgatgg tcctgcatat gaatattatc ctgatggcaa actcatgcag
     4501 gaaaatttct atcagcaaag tgtgttgatt ggaaacgata ccagttacta ccaatccgga
     4561 aaaataatct ctatacacaa tagaaattcc cgtggtcagt atgatggagc taacgaacgt
     4621 tatagtgaag agggtaagtt acagtctaaa tccgtctata aagacggaaa acaaatttct
     4681 gttcaaacct ggtatgaaaa tgggcagaaa gaagaggaga aacattttga tgagcaaggc
     4741 caattaaatg gtctggttaa gcaatggtat aaaaatggta atcttgcaaa atcacaaaac
     4801 tataaacatg acatactcga tggtaacagt gaagagtggt acgaaaatgg cattcctgaa
     4861 agtttgtacc cttataagaa tggaaaaacc gacggcattg ccaaatcatg gaataagtat
     4921 ggtaaattaa cctattcaat cgaatacaaa aatagcgtag agaatggggc atatcgtaac
     4981 tggagcaaaa acacaggtaa actaaccaaa gaaaccctgt acgtcaatgg tatcagacag
     5041 ggagtggaaa aagagtttaa cgatcgcaca ggtaaactat taacctccac gcaatatgtt
     5101 aataataaaa gacatggtac ggaggaaact tatgatcaaa acggaataaa atatattaca
     5161 tgctatcaaa atgatcagaa attatcgtct ttagacaatc caacacaaat aaaagataat
     5221 gcaacaacag gcgattccag tgctcaattt gcattaggga aatacgaatt catctgtgca
     5281 aatatagatg aagggattaa atggttaacc aaatctgcag agcaaaaaaa acactgatgc
     5341 gatatacttt ttagctacag cctacaaa
//
LOCUS       DAJVQH010000191         5340 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJVQH010000191 DAJVQH010000000
VERSION     DAJVQH010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.193"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OQM87_003920"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OQM87_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCS1509855.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OQM87_003921"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OQM87_003921"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCS1509856.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OQM87_003922"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OQM87_003922"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCS1509857.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="OQM87_003923"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="OQM87_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCS1509858.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1309 a         1290 c         1457 g         1284 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 cgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gctcccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac tctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcagc aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt tcgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaaattaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
//
LOCUS       DAJVQH010000192         5308 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJVQH010000192 DAJVQH010000000
VERSION     DAJVQH010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.194"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..607
                     /locus_tag="OQM87_003924"
                     /pseudo
     CDS             1..607
                     /locus_tag="OQM87_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            671..1597
                     /gene="glsB"
                     /locus_tag="OQM87_003925"
     CDS             671..1597
                     /gene="glsB"
                     /locus_tag="OQM87_003925"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCS1509859.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1564..1956
                     /locus_tag="OQM87_003926"
     CDS             1564..1956
                     /locus_tag="OQM87_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCS1509860.1"
                     /translation="MDETVRAFGLLRQSLDPLFARLSRSKFRSRFRLGMKERQYCLEK
                     GAPVIEQHAADFVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIP
                     QGVSLSEEQQRYIVAVIYHWLVVQMNQP"
     gene            2095..3513
                     /locus_tag="OQM87_003927"
     CDS             2095..3513
                     /locus_tag="OQM87_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS1509861.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3740..5191
                     /gene="uxaB"
                     /locus_tag="OQM87_003928"
     CDS             3740..5191
                     /gene="uxaB"
                     /locus_tag="OQM87_003928"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCS1509862.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1225 a         1236 c         1431 g         1416 t
ORIGIN      
        1 cccatcattc ttgcaggtaa cggctactta cttaaacatg cgccgaatgt gatgggctgt
       61 gctcagctca ttgcccaggt gtttaaagat gcgggaatcc cgcaaggcgt atatggctgg
      121 ctgaatgccg acaacgacgg tgtcagtcag atgattaaag actcgcgcat tgctgctgtc
      181 acggtgaccg gaagtgttcg tgctggagcg gctattggcg cacaggctgg agcggcactg
      241 aaaaaatgcg tactactatc cgccaacggt tctggcgaat gttaccccag aaatgaccgc
      301 gtttcgggaa gaaatgtttg gccccgttgc tgcaatcacc attgcgaaag atgcagaaca
      361 tgcgctggaa ctggctaatg atagtgagtt cggcctttca gcgaccattt ttaccaccga
      421 cgaaacacag gccagacaga tggcggcacg tctggaatgc ggtggggtgt ttatcaatgg
      481 ttattgtgcc agcgacgcgt gagtggcctt tggtggcgtg aaaaagagtg gctttggtcg
      541 tgagctttcc catttcggct tacacgaatt ctgtaatatc cagacagtgt ggaaagaccg
      601 gatctgacgc gacatattca gctctgatat actcgcaggt cttttcagac ctgcggtcca
      661 ggagtagaaa gtggcagtcg ccatggataa tgcaatttta gaaaacatct tgcggcaagt
      721 gcggccgctc attggtcagg gtaaagtcgc ggattatatt ccggcgctgg ctacagtaga
      781 cggttcccga ttggggattg ctatctgtac cgttgacgga cagctttttc aggccggaga
      841 cgcgcaagaa cgtttttcca ttcagtctat ttccaaagtg ctgagtctcg ttgtcgccat
      901 gcgtcattac tccgaagagg aaatctggca acgcgtcggc aaagatccgt ctggatcacc
      961 gttcaattct ttagtgcaac tggaaatgga acagggtatt ccgcgtaatc cgttcattaa
     1021 tgccggtgcg ctggtggtct gcgatatgtt gcaagggcga ctaagcgcac cacggcaacg
     1081 tatgctggaa gtcgtgcgcg gcttaagcgg tgtgtctgat atttcatacg atacggtggt
     1141 agcgcgttcc gaatttgaac attccgcgcg aaatgcggct atcgcttggc tgatgaagtc
     1201 gtttggcaat ttccatcatg acgtgacaac cgttctgcaa aactactttc attactgcgc
     1261 tctgaaaatg agctgtgtag agctggcccg gacgtttgtc tttctggcta atcaggggaa
     1321 agctattcat attgatgaac ctgtggtgac gccaatgcag gcgcggcaaa ttaacgcgct
     1381 gatggcgacc agtggtatgt accagaacgc gggggagttt gcctggcggg tgggactacc
     1441 ggcgaaatct ggcgttggtg gcggtattgt ggcgattgtt ccgcatgaaa tggccatcgc
     1501 agtctggagt ccggaactgg atgatgcagg taactcgctt gcgggtatcg ccgtccttga
     1561 acaattgacg aaacagttag ggcgttcggt ttattaaggc agtctctcga tccactcttc
     1621 gcgcgtttat cccgttcaaa atttcgctct cgctttcgtc tgggcatgaa agagcgtcag
     1681 tattgcctgg agaaaggcgc gccagtcatc gaacaacatg cggcggattt tgtcgctaaa
     1741 cgtcttgctc cggcattacc ggctaatgac ggtaagcaac cccccatgcg cgggcatccg
     1801 gtgtttatcg ctcagcacgc caccgcgacc tgctgtcgcg gttgtctggc taagtggcat
     1861 aacattccgc agggtgtttc gttaagcgaa gaacaacaac gttatatcgt ggcggtaatc
     1921 tatcactggc tggttgtaca gatgaatcag ccatgagaat tgcggaaaac gataaaatag
     1981 cattcccagg gtacctttat ttgccgccgc tgttagcgaa catgctatcc tttatgagaa
     2041 tttacttatc acgttgagat aattgccacc cgcaattgta tggacagata attaatgcat
     2101 gtacacccca tttcaacttt ccgtttgttc caggaaggtc atctgctacg taatagcatc
     2161 gctatttttg cgctaaccac gctgttctat tttattggtg cagagttacg gctggttcac
     2221 gaactttctc ttttctggcc gctgaatggc gtaatggcgg gggtgtttgc ccgctatgtc
     2281 tggcttaatc gactgcatta ctatgcgatc agttatgtgg cgatgctggt ttatgatgcc
     2341 ataaccaccg aatggggact ggtttcactg gttatcaatt tctccaatat gatgtttatt
     2401 gttaccgtcg ccttactggt cgtgcgggat aagcgtctgg ggaaaaataa gtatgagcca
     2461 gtaagtgcct tacggctatt taattactgt ctgattgccg cattattatg cgctattgtc
     2521 ggggcgattg gttcggtcag tattgatagt ctggatttct ggcctttgct tgcggactgg
     2581 ttcagtgagc aattctcaac gggcgtgttg atcgtgcctt gtatgctgac gttggctatt
     2641 cctggagtgc tgccgcgctt taaagcagaa cagatgatgc ccgccatcgc gcttattgtg
     2701 tcggttattg cctcggtagt cattggcgga gcggggagtc tggcgttccc gctgcctgca
     2761 ttaatctggt gtgcagtgcg ctatacgccg caggtaacat gtctgttgac ctttgtcacc
     2821 ggtgcggtgg aaatcgtact ggtggcaaat tcggtgattg atatctcggt cggttcgccg
     2881 ttctccattc cggaaatgtt ctccgcacgt ctcggtattg ccacgatggc gatatgccca
     2941 attatggttt cttttagcgt ggcagcgatc aatttgctaa tgaagcaagt tgcgctgcga
     3001 gccgactttg attttctgac tcaggtttac tcacggtccg gtctttatga ggcgctgaaa
     3061 agtccatcgc tgaaacagac acaacatctg acggtcatgc tgcttgatat cgactatttc
     3121 aaaagcatta acgataacta tggacatgaa tgtggcgata aagtgttaag cgtgtttgcc
     3181 cggcatattc agaagattgt cggtgataag gggctggtgg cgcgcatggg cggcgaagaa
     3241 tttgctgttg cagtgccgtc ggtgaatcct gtcgatggtc tgctaatggc ggaaaaaatc
     3301 cgtaaaggcg ttgaactgca accattcacc tggcaacaaa aaacgcttta tctcacggta
     3361 agtattggcg tcggtagtgg tcgcgcatcg taccgaacgc tgaccgatga ttttaataaa
     3421 ttgatggtcg aagccgatac gtgtctgtat cgctcgaaga aagatgggcg caaccgtacc
     3481 agcactatgc gttacggtga ggaagttgtc tgacatcttt attcattccg cgcgatttga
     3541 atgcaatgtg ttctaccact ttatgatcca ggaaaaggcg tatttcctcg attaaaccat
     3601 gatccgcgcc acacttttct cataaaactg ctcattaatc atcaggagtt gatcaaaaca
     3661 ggggttcggc gtattaaagt ttgctaacct gtcgcaccaa ttcaaacagg ttgtatgact
     3721 aatcagaagg gaacccattg tgaaaacact aaatcgtcgc gattttcccg gtgcacagta
     3781 tccagaacgt atcattcagt ttggtgaagg taacttcctg cgcgcctttg ttgactggca
     3841 aatcgatctc ctgaatgagc acaccgatct gaattctggc gtggtcgttg ttcgtccgat
     3901 tgaaacttca ttcccgccgt cactgagcac gcaggatggt ctgtacacca ccattatccg
     3961 tggcctgaat gagaaggggg aagcggtcag cgacgcgcgt ctgattcgct cggttaatcg
     4021 tgaaatcagc gtctacagtg aatacgatga attcctgaaa ctggcgcaca acccggaaat
     4081 gcgctttgtt ttctctaaca ccaccgaagc gggtatcagc taccatgcgg gcgataaatt
     4141 cgatgacgcg ccagcggtaa gctatccggc aaaactgacc cgtctgctgt tcgaacgctt
     4201 tagccatttc aacggtgcgc tggataaagg ttggatcatc attccgtgtg agttgattga
     4261 ctacaatggc gacgcgttgc gtgaactggt gctgcgctat gcgcaagagt gggcactgcc
     4321 agaagcattt attcaatggc tggatcaggc taacagcttc tgttctacgc tggtggaccg
     4381 tatcgttacc ggttatccgc gcgatgaagt ggctaaactg gaagaagaac tgggttatca
     4441 cgatggtttc ctcgacaccg ctgaacattt ttacctgttt gtgattcagg ggccgaaatc
     4501 cttagcgact gagctgcgcc tggacaaata tccgctcaac gtgctgattg ttgacgatat
     4561 taaaccgtat aaagaacgca aagtggcaat cctcaacggt gcgcacaccg cgctggtgcc
     4621 ggtggcgttc caggcagggc tggataccgt aggtgaagcg atgaacgatg ctgaaatttg
     4681 cgcattcgtt gaaaaagcca tctacgaaga aattattccg gtactggatt tgcctcgtga
     4741 tgaactggaa tctttcgcca gtgcagtcac cgggcgtttc cgtaacccgt acattaagca
     4801 tcagctgctg tctatcgcgc tcaacggtat gaccaagttc cgcacccgca tcctgccgca
     4861 gctgctggca gggcagaagg caaacggcac acttccggcg cgccttactt tcgcattagc
     4921 ggcattgatt gcgttctatc gtggtgagcg taacggagaa acatatccgg tacaggacga
     4981 tgcacactgg ctggaacgtt atcagcaact ctggagccag catcgtgacc gtgttatcgg
     5041 tacccaggaa ctggtagcga ttgtgttggc agaaaaagac cactgggagc aggacctgac
     5101 gcaagtgcct ggtctggtcg agcaggttgc taatgacctg gatgcgattc ttgaaaaagg
     5161 gatgcgcgaa gctgtacgtc cgttgtgcta ataacaccgt tacttctgaa cccggtttcg
     5221 accgggtttc ttatttcact atattagtta caacatccta tatgccttta tcattaacag
     5281 gtgtcacatg cagcgcctgt taatgttg
//
LOCUS       DAJVQH010000193         5291 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJVQH010000193 DAJVQH010000000
VERSION     DAJVQH010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.195"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..789)
                     /locus_tag="OQM87_003929"
     CDS             complement(<1..789)
                     /locus_tag="OQM87_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCS1509863.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLG"
     gene            1035..1661
                     /gene="gstB"
                     /locus_tag="OQM87_003930"
                     /pseudo
     CDS             1035..1661
                     /gene="gstB"
                     /locus_tag="OQM87_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(1658..2772)
                     /gene="yliI"
                     /locus_tag="OQM87_003931"
                     /pseudo
     CDS             complement(1658..2772)
                     /gene="yliI"
                     /locus_tag="OQM87_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2883..3266)
                     /gene="bssR"
                     /locus_tag="OQM87_003932"
     CDS             complement(2883..3266)
                     /gene="bssR"
                     /locus_tag="OQM87_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCS1509864.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3479..4804
                     /gene="rimO"
                     /locus_tag="OQM87_003933"
     CDS             3479..4804
                     /gene="rimO"
                     /locus_tag="OQM87_003933"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCS1509865.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4851..>5291)
                     /locus_tag="OQM87_003934"
     CDS             complement(4851..>5291)
                     /locus_tag="OQM87_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS1509866.1"
                     /translation="LDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNINDTFGH
                     LLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEIVHFQVG
                     SINLSTSIGVAHSNKCTTVERLKMLADERLYKSKKNGRAQISWQ"
BASE COUNT         1319 a         1374 c         1273 g         1325 t
ORIGIN      
        1 ccccagcact acggagatta aacgcatatc accctgggta gccgaagcaa ccagattata
       61 tcccgcacct gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag
      121 cagacggtta cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat
      181 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
      241 ggtactgaac tgccccggcg catccaggcc gtgcaccgtc tggaaggtag tgttggtcag
      301 ccccagtttt ttggcataac cattcatcag accaataaag gactcctggc tcccggcaac
      361 gtaatcggcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
      421 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
      481 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
      541 atcggcctta agcacctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
      601 atccagtttc tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
      661 aatccatgca cgcgcatcca cgctcggcgc ttcaacggtt tgttcggccg cgaatgccgt
      721 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga gcacgaagga gagaggagta
      781 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaat acgttatatc gccgtatgat
      841 aagttcggtg attaataacg cacacacaaa cttaaagaaa ccgtaagttg gtaaagtttt
      901 taaagtttat gcaatatcct gccgcttcgc tgcttctggt gtcatttttt tgccagctgt
      961 tgcttaaaag ttaactttac ttcaccatcg gagtcctgtt cagtctgtgg ctgcctgtcg
     1021 gaaaaggggt gagtatgatt acgctgtggg gtcggaataa ttcaactaac gtaaaaaaag
     1081 tattgctgac gctcgaataa ctggaactac cttatgagca aattctcgcg ggccgtgagt
     1141 ttgggataaa tcacgatgct gattttctgg cgatgaaccc taacgggctg gtgccgttgt
     1201 tgcgtgacga cgaaagtgat ctcattcttt gggaatcaaa cgccattgtc cgctatctgg
     1261 cggcacagta cgggcaaaaa cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg
     1321 aaaaatggat ggactgggca aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg
     1381 gattagtcag aacaccaccg gaagagcgcg atcaggccgc cattgatgcc agttgcaaag
     1441 agtgcgacgc cctgtttgcc ctgctcgatg cggaactggc aaaagtaaaa tggttctctg
     1501 gcgacgagtt tggtgtgggc gatatcgcta tcgcaccgtt catctacaat ttgtttaacg
     1561 ttggcctgac ctggacaccg cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac
     1621 gccccgcagt gcgcaaagtg gtgatgattc ccgttagcta attgcgtggg ctaactttaa
     1681 gtaattcccc actggactcg tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa
     1741 catcacgaat tcgctgccct ctgtccgtta aaatacggcc atcttctgtc actttgtcgc
     1801 cgttgacgct catcacaatg acatctttat ctttcagcgc ccaataaata atttttgctg
     1861 ccactggggg aatttatcgc tgttatagaa ggccatgccg ctcacagcgg gcgaatcttt
     1921 ccagtaaaaa acaggttgct cggtcccggc gacgatctcc cctttcgctt ccggtatctt
     1981 aaagcctgaa tagttgattc cccaggttgc cagcggccag ccgtagtttt tgcctttttg
     2041 cgggatatta atttcatcac caccgcgcgg gccatgttca ttcagccaca gtgcattact
     2101 ccacggattc atcgccattc cttgcggatt acgaatgcca taagaccaga tctcgccgcg
     2161 cgcaccggat tcctttataa aaggattatc atccgggatt tcgccctggt cggtcagacg
     2221 caccagtttg ccctgtaatt tatccagatc ctgcgccgtc gggcgctgat tgttttcgcc
     2281 cagagcaata aaaagataac ctttaccgtc gaataccagc cgcccgccaa aatggttgcc
     2341 ggtagacagt tttggcatct ggcggaagac ggtgcggaag tcggtcactt ttgagagatc
     2401 atcacttaag cggccataac ccacagcagt tccggctttg ccatcatcgc caacttcgga
     2461 ataacttaac cagatgcggc gagactgagc aaaatcaggc gctaaaacca cgtccagcag
     2521 gccgccctgc ccgtgcgccc aaacgtccgg aactccggaa agcggcgcag ataatccttt
     2581 tcctgcttgc cagtgacgca actcgccgcc gcgcagagtg attaacatac cgtgattatc
     2641 gggtaaaaag gccagtgccc agggatggtc gagtttgtct tgcagtactt cgacatttac
     2701 cgttgcagga gccgcccaga gagcggaaga aagacaaata aggggcacaa ggaaaaagga
     2761 ttgtcgatgc ataacgcctc cggattgact ctggcttaaa gcgtagtcag tggaggagat
     2821 aacaaattca tttttacaaa aacttaaaca tgaaggggga gacgctttct cccccttagt
     2881 tttcaggcct tctcaagcat ggcgtgcttc tgcaggctct ggatactcag cgttaagctc
     2941 atcagacaat tttcaagctt atcggcgttg acggtaataa cagtcgggca atcatggtgc
     3001 ccactcatca aacatactgc ggctgtcgct aatgcttctt cagcatgatg aagagcactc
     3061 cactcttcct gatccagatg aagattcaac cgcagcgatt tatcgtgcag ttcgcgattc
     3121 agtttaaaaa agttatctcg tagatgattg ctttcgctga cggacatgta tccttttgcc
     3181 ttcctcagtt gcacgtatgc agcgaggtca acgcgtgcgt cgatcaaccg gttcagcaga
     3241 tcgattcgct gtctgtcaac gaacatactt cgatcctcct ctttccagcc ttgttgcaat
     3301 taaatagtat ggctattttt tgagcaaaat gctgtctagg atcaattctt cacgccgaat
     3361 taacaaaatt tacatttcct tcgcctccgc cagcgaatga gattgcgata caaattttat
     3421 ccagaactct gtagaatccc tgccctgacc tttcctgtaa ctgaatattt tttaagctat
     3481 gagcaaagta actccccagc cgaaaatcgg ctttgtttcc cttggctgtc cgaaaaacct
     3541 tgtcgattca gagcgtattc tcaccgaact ccgcactgaa ggttatgacg tggtaccgag
     3601 ctatgacgat gcggacatgg tgatcgtcaa cacctgcggc tttattgaca gcgcggtaca
     3661 agaatcactg gaagccattg gtgaagcgtt gaatgaaaac ggcaaggtaa ttgtgaccgg
     3721 ttgtctgggg gcaaaagaag atcagatccg cgaagtccac ccgaaagtgc tggaaatcac
     3781 cgggcctcat agctatgagc aggttctgga gcacgttcat cactacgtgc caaaaccgaa
     3841 acacaaccca ttcctgagcc tggtgccaga acaaggtgtg aaactgacgc cgcgtcatta
     3901 tgcctatctg aaaatttcgg aaggctgtaa tcaccgctgc accttctgca ttattccgtc
     3961 tatgcgcggc gacctggtga gccgtccgat tggcgaagtg ttaagtgaag cgaaacgtct
     4021 ggtagatgcg ggcgttaaag agattctggt gatctcgcag gatacttccg cctatggcgt
     4081 tgatgttaaa catcgtactg gcttccacaa cggcgagccg gtaaaaacca gcatggtcag
     4141 cctgtgcgaa cagttatcga aactggggat ctggacacgt ctgcactacg tttaccctta
     4201 tccgcatgtg gacgacgtca tcccactgat ggcagaaggc aaaatcctgc cgtatctgga
     4261 cattccgttg cagcacgcca gcccgcgcat tctcaaactg atgaagcgtc cgggttctgt
     4321 agatcgccaa ctggcgcgca tcaaacagtg gcgcgaaatc tgcccggaac tgaccctacg
     4381 ctcaaccttt attgtcggct tccctggcga gacggaagaa gatttccaga tgctactcga
     4441 cttcctgaaa gaagcacgtc tggatcgcgt tggctgcttt aaatacagcc cggttgaagg
     4501 tgcagacgcc aatgccctgc ctgaccaggt tccggaagaa gtgaaagaag aacgctggaa
     4561 ccgtttcatg cagctgcagc agcagatttc cgccgagcgc ctgcaagaga aagtgggccg
     4621 tgaaattctg gtgattatcg acgaagtgga cgaagaaggc gcgattggtc gcagcatggc
     4681 agatgcaccg gaaatcgacg gcgcggttta tctcaatggt gaaaccaaca ttaagccggg
     4741 tgatatcctg cgtgtgaaag tcgagcacgc cgatgagtac gatttgtggg gtagccgggt
     4801 ttaattaatt caggccgggc aaaatcattt gtccggcctg cgaataatga ttactgccag
     4861 ctaatctgtg ccctgccgtt tttcttactc ttatacagcc gctcatccgc cagcattttc
     4921 aagcgttcga cggtagtaca tttatttgaa tgcgctacac cgatactggt gcttaaatta
     4981 atgcttccca cctggaaatg aacgatttct tttagtatcg atagcaactc ttccagcgtg
     5041 ccgccagtat aaatcaccgc aaattcgtca cctccgaagc ggtagacttt acctttatct
     5101 accgggatct gtgatttaag gattttcacc actttcatca acacttcatc gcccagcaaa
     5161 tgaccgaagg tgtcgttaat gtttttgaat ttatcgacgt caaacagaca caaataaccg
     5221 cctttttccg gcagcgcttt taaatcttca tcaaaggatt ttctgcccat tgctttggtc
     5281 aggtcgtcca g
//
LOCUS       DAJVQH010000194         5235 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJVQH010000194 DAJVQH010000000
VERSION     DAJVQH010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.196"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..168)
                     /locus_tag="OQM87_003935"
     CDS             complement(<1..168)
                     /locus_tag="OQM87_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_085938460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1509867.1"
                     /translation="MRQRAVRMVLESQGEYDSQWAAICSIAPKTGCTPETLRVWVRQY
                     ERDTGGGDGGLT"
     gene            complement(253..686)
                     /locus_tag="OQM87_003936"
                     /pseudo
     CDS             complement(253..686)
                     /locus_tag="OQM87_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(747..1228)
                     /locus_tag="OQM87_003937"
                     /pseudo
     CDS             complement(747..1228)
                     /locus_tag="OQM87_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
     gene            1394..2506
                     /locus_tag="OQM87_003938"
     CDS             1394..2506
                     /locus_tag="OQM87_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HCS1509868.1"
                     /translation="MKITKLTTFIMHVPVTKNIIGDSTHSITHWGMPGVMIETDTGLV
                     GYGYTGTHADITTDRLITTIIKDAFGPMLIGEDASEVRYLHRKMSRSSTNIWVGRGGL
                     MQMALSAIDIALWDLKAKMVEQPLWKVFGGSQHSRVLAYNTDCGWLVRNTAELVDDCK
                     KMIFEEGFSAIKMKIGKPDPREDLKRIEAVRKAIGDDVNLMVDANAKWDISTAKQYGA
                     KLADFDITWFEEPMWHDDIASHRTLSGHISTPIALGELLYQFDSFKEFVLAGAVDYLQ
                     PDATRCGGLTAVWEIADLGQAFNLPVTPHHGDMMQAQLHLVMAHPSCSLLEFIPWTLG
                     CFVDPVEVKEGYYSLPTAPGAGTTLRPEALEHFSVR"
     gene            2578..3945
                     /locus_tag="OQM87_003939"
     CDS             2578..3945
                     /locus_tag="OQM87_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1509869.1"
                     /translation="MPSHTDQYLSLAIQKSIRRIIPMVILMFILAYLDRSNIGFAKQE
                     FQLDTSLSDTAYAFGAGIFFIGYALFEVPSNILLYRIGARVWLARIMVTWGLVSAAMM
                     FAHTETVFIFLRFLLGISEAGFFPGVILYLTFWFPQHIRARVTGYFLFGAPLAFIIGG
                     PLSGSLLSLEGSVLSFGLHGWQLMFLVEGLLASAVGIWVFFYLDDHPEKAKWLTPQEK
                     AALSQTLNKEEQVKLAHGPKGAWQALLDVRVLYLCLIWFTVQVCGYGIYFFLPTQIAT
                     LIGTKVGLLVGFVTAIPPLCAAIAVYYVPRISEKLQERRYVASITFMSGAIGIAVSGL
                     ADSIPLVAIVALCVAAAGHLAMQPLYWTFPSSYLGGAAAASGIALINSIGNLGGFVAP
                     NLRVWAEHTFNSAQAGLYLLAMVTATGSLMILTLRHFGIEKTPRTSSTSPNLPASGDN
                     LHHSD"
     gene            3995..4738
                     /locus_tag="OQM87_003940"
     CDS             3995..4738
                     /locus_tag="OQM87_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCS1509870.1"
                     /translation="MVKILAVAQSHESIVSMDEINSILLSSSWFRALPDYLIEQMLSR
                     TSLYQYNDGEIIHYRGAEPLGLYAVITGSVKVSSISADGKECIFRYLSPGNWFGEIGM
                     IDKCPRTHDARATGKTTLLTIKPHDVEFLLKNYPILHRFLALLLCKVVRNAFTMLHDC
                     TLLSVSARLAKRLVSLSEGYGEPHERGTLINLYLTQDDLATIINTSRQTINKRLVEWE
                     KLGWIDARYGKIVIININALRQLYADEDE"
     gene            complement(4990..>5235)
                     /gene="tnpB"
                     /locus_tag="OQM87_003941"
     CDS             complement(4990..>5235)
                     /gene="tnpB"
                     /locus_tag="OQM87_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1509871.1"
                     /translation="VTDMRKSFNGLGEQVQHVLNDNPFSGHLFIFRGRRGDTVKILWA
                     DADGLCLFTKRLEEGQFIWPAVRDGKISITRSQLAML"
BASE COUNT         1243 a         1275 c         1251 g         1466 t
ORIGIN      
        1 ggtgagccct ccatctccgc ccccggtatc ccgctcatac tggcgaaccc agacacgcag
       61 agtctccggc gtacagccag tctttggagc aatggagcaa attgctgccc attgtgagtc
      121 atattcgccc tgactttcca gaaccatacg aactgcccgt tgacggattt cgggggaaaa
      181 cgtgtatttt tagtcatcct gtttacctct ttctcaggga gtttagtctc caggatttcc
      241 ggggcggttc accatcacgg catcagtgac ctttgatatc agtgccggtg aaacatcagc
      301 gtcatacagg tctttgaacg cggcggctat ctcacgggtg gttatccctt tggcatacaa
      361 cgagaggatc tggttatcca tcctggtaat acggggctga tttttcttca cctgttgtgg
      421 ttcgaagcta ccgtcacgat cgcgcggagt acgcagttcc agtggaccgt cgcctgtgat
      481 aacggtcttt gtggaataac cgttgcggga gttagctcct ggtctggact gatttttcca
      541 tacccaggat ggtgtgtcat ctctgcgttg agagcggctt caacgctgat ctttttcagt
      601 agacggtcaa actggctgag gtcttcaggg gttttgaggt ttttggccag ttcgttagcc
      661 agagtctgta actatttttc gtccataaat taaccttcat ttgatgttga attgaacata
      721 tcaaaatcag gcaattacac aaatctataa agcgcctcgt catcatcttt accacgcgtc
      781 atgaccattt tcaccatttc agaaggcggt aatttttctg ctcccgttca gtcaggacga
      841 tgccatcggg tacttgtttc acttaaaacc aggcagtgcg aatagcttca aggtgacggt
      901 agaaccctag gcaatttcac ggctggcaca aacatggtct tgccttttac ggtgaaacct
      961 tcggctacgc gtttttcata atgtctggca aaatcttccg cgagggattt taacctatct
     1021 tcatcaccca gaactacatt catggttgcg gtagcttttt tactttcatc gatgtgccac
     1081 tcattggtgc ctgcgtttgc gtatttttcg taatactttt cgattttttc cagtttgctg
     1141 tactccagga tcactttagc cgcatggtct tcatgatgcg tacagagatc tcatcctgaa
     1201 aagctccggt catggcccag ttccctatat gaaaacttgt cgactgggtg tggtccaaat
     1261 tcgagatcta cttcaaaaaa atacaattat tccttctctc ttctgatgat gtgtcaacca
     1321 tttgacagcc ctgctgaagg ccggttgcta cattgcgccc atcaatcaag atatacagac
     1381 acaggaaact gcaatgaaaa tcactaaact cactaccttt attatgcatg taccggtaac
     1441 taaaaatatt atcggtgact ccacacacag cattactcat tggggaatgc cgggcgtaat
     1501 gatcgagaca gataccggcc ttgtaggtta cggttatacc ggaacccatg cagacattac
     1561 taccgaccgt cttatcacca ccattattaa agatgccttc ggcccgatgt tgattggaga
     1621 agacgccagt gaagttcgct atctccaccg taagatgtca cgcagctcga ccaatatctg
     1681 ggtgggccgc ggtgggttaa tgcaaatggc gctttcagca atcgatatcg ccctgtggga
     1741 cctgaaagcc aaaatggtgg agcaaccttt gtggaaagtc tttggcggtt ctcaacattc
     1801 ccgtgttctt gcctacaaca ccgactgcgg ctggctggtt cgtaacacgg cggagttagt
     1861 ggatgactgc aagaagatga tttttgagga gggattttcc gccatcaaga tgaagatagg
     1921 caagcccgat ccgcgagaag atctcaaacg aatcgaagcc gtcagaaaag cgataggcga
     1981 tgacgtcaat ctgatggtcg acgcgaatgc aaaatgggac atcagcactg ccaagcagta
     2041 cggtgccaag ctggctgatt tcgatattac ctggtttgaa gagccgatgt ggcatgatga
     2101 tatcgcaagt caccgcacgc tgtccgggca tatcagtacg ccgattgcgt tgggtgagtt
     2161 actctatcag tttgattcct ttaaagagtt tgtactggcc ggtgcggtgg attatctcca
     2221 gcccgatgcc actcgctgcg gtggactgac cgccgtatgg gaaatagccg atctcggtca
     2281 ggcgttcaat ctgccggtca caccacatca tggtgatatg atgcaggcac aattacatct
     2341 ggtaatggca catccctctt gctctctgtt ggagtttatt ccctggacgc tgggctgctt
     2401 tgtcgatccg gttgaggtca aagagggata ttactctctt cccaccgcgc cgggtgccgg
     2461 cactacctta cgcccggaag ctcttgagca ctttagcgtt agatgagaac acttgtatcg
     2521 cccggttatg caatgaccgg ggcaaaataa taatgacaaa attctaagag gtgatttatg
     2581 cccagccata ccgatcagta tttatcactt gcaattcaaa aatccatacg tcgcatcatt
     2641 ccgatggtca tactaatgtt tattctggcc tatctggatc gctccaatat cggctttgct
     2701 aaacaagaat ttcaactgga taccagtctg agtgacaccg cctatgcttt cggcgccggg
     2761 atcttcttta ttggttatgc cctgttcgag gtgcccagta atattctgct ttatcgcatc
     2821 ggcgcccgtg tctggctggc ccgtatcatg gtgacctggg ggctggtttc cgctgcaatg
     2881 atgttcgccc acactgagac cgtatttatt ttcctgcgct tcttgcttgg catcagtgaa
     2941 gcaggtttct ttcctggagt tattctttat cttacattct ggttccccca gcacatccgc
     3001 gcacgcgtca ccggctattt cctgtttggt gccccactag cttttatcat cggcggtccg
     3061 ctttccggct cgctactctc gcttgaagga tcggtactgt cgtttggtct ccatggctgg
     3121 caactcatgt tcctcgttga ggggttactg gcctctgccg ttgggatctg ggtgttcttc
     3181 tatcttgacg atcacccaga gaaagccaaa tggttaactc cacaagagaa agcggcgctt
     3241 agccagactc taaataagga agagcaggtg aaacttgcac atggtccaaa aggcgcatgg
     3301 caagcactgc tggatgtacg cgtcctctat ttatgcctga tctggtttac ggtgcaggta
     3361 tgtggttatg gaatttactt ctttctgcca acacaaatcg ccaccttaat agggacaaaa
     3421 gttggtttat tagttggctt cgtcaccgct attccacctt tgtgcgctgc catcgcagtg
     3481 tattacgtac cacgtatatc ggaaaaactg caggaacgtc gttacgttgc atcaatcaca
     3541 tttatgtccg gtgcaatagg cattgccgta tctggccttg ccgatagtat tccactggtc
     3601 gctattgtcg cgctgtgcgt tgccgctgca ggacatctgg cgatgcagcc actgtactgg
     3661 acatttccat cgtcttatct cggcggtgca gcagctgctt ccggcattgc gttaattaac
     3721 tcgataggca atctgggggg gtttgtagcg ccgaatcttc gcgtctgggc ggaacatact
     3781 tttaattccg cacaggcagg actttactta ctggcaatgg taacagctac tggtagcctg
     3841 atgattctga cattacgtca cttcgggatt gaaaaaacgc cccgtacatc atccacatct
     3901 ccgaatctcc ctgccagcgg ggataatctg catcactctg attgacagga aatgctacat
     3961 tgctacggtc atgattaaga tcaggataac aacaatggtt aagatattgg ccgtagcaca
     4021 atcacatgaa tcgatagttt ctatggacga gatcaatagt atccttctta gtagtagttg
     4081 gtttcgggcg ctacctgatt atcttattga acagatgttg tccaggacca gcctgtatca
     4141 atacaacgat ggagagatta ttcactatcg tggtgctgag ccgctcggat tatatgcagt
     4201 cattactggc tccgttaaag tgagctcaat ttctgctgac ggtaaagagt gcatcttccg
     4261 ctatctttcg ccggggaact ggtttggtga aataggtatg attgataaat gtccgcgcac
     4321 acacgatgcc agagcgacgg gtaaaactac actgttaacg atcaagcctc atgatgttga
     4381 atttcttctg aaaaattatc caattctcca tcgttttctg gcgttgctgc tttgcaaagt
     4441 cgttcgaaat gcctttacga tgttacatga ctgtacgcta ttatctgttt ctgcacgctt
     4501 ggcgaagcgt ttagtgagtc tgtcggaagg gtacggcgaa ccacacgagc gagggacatt
     4561 aatcaatctt tatcttacgc aggacgatct ggcaacgata atcaatacga gccgacaaac
     4621 gatcaacaaa cggctggttg aatgggaaaa attgggatgg atagacgcca gatatggcaa
     4681 aatcgtgatc atcaatataa atgcactcag gcagttatat gctgatgaag atgagtaatt
     4741 ggctgtggat gagcctgttt accattctgt atacatgctt tttcttaaaa gtgactgtcc
     4801 aggcggtcat caaattcgat aataaattgc tattcgccag tccctctgcg gcattgtcca
     4861 tttctgcgac gcgaccagga tgccagccat ttcactttta ctgactcgcc catcgggaac
     4921 gccttacgtt tcctaatggc atgtcggatc acacagttcg taagcgtcaa tacggcgctc
     4981 cgttaacgtt tacagcattg ccagttgcga gcgggtaatg gatatcttac cgtcacgcac
     5041 cgcaggccag ataaactgac cttcctcaag gcgtttggtg aacaggcaca gaccatcagc
     5101 atcagcccaa agaattttaa cggtgtcacc ccgtcggcca cggaagataa acaggtggcc
     5161 ggagaaggga ttatcattca gcacatgttg tacctgttct cccagtccgt tgaaggattt
     5221 acgcatatcg gtaac
//
LOCUS       DAJVQH010000195         5164 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJVQH010000195 DAJVQH010000000
VERSION     DAJVQH010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.197"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            571..780
                     /locus_tag="OQM87_003942"
     CDS             571..780
                     /locus_tag="OQM87_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509872.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTD
                     EHVPLYIRRLEYYRLLYRPKQTESY"
     gene            complement(846..1379)
                     /locus_tag="OQM87_003943"
     CDS             complement(846..1379)
                     /locus_tag="OQM87_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509873.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(1382..2308)
                     /locus_tag="OQM87_003944"
     CDS             complement(1382..2308)
                     /locus_tag="OQM87_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCS1509874.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTAPERVKSPSRGFSMGF"
     gene            2485..2883
                     /locus_tag="OQM87_003945"
     CDS             2485..2883
                     /locus_tag="OQM87_003945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509875.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQALKELKYDLETSPNPDA"
     gene            complement(3087..4001)
                     /locus_tag="OQM87_003946"
     CDS             complement(3087..4001)
                     /locus_tag="OQM87_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCS1509876.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
BASE COUNT         1436 a         1349 c         1106 g         1273 t
ORIGIN      
        1 aaaaaaaaac gacaagttat acaataaaac cagtactatc catacagcta attacatata
       61 aatactatta aaccatctgg acattaaaaa taaactctta taacataaaa ttaatgcggt
      121 tccggtacca tatgtcaggg ataatgtgca aatggaggta tcggaactgc atttcagttc
      181 attattaatt aatgacaaaa aaaacctctg cgagcgcaga gggggtaagg aatgttcccg
      241 gtcataatat gtcgatagtg tttatataca tacagaacaa gaacaacaac cagccaacta
      301 ataaatattt tattttttag agctgaaacc ttaaagctca cttccattcc atacattgtt
      361 ataaacctta tgttgaaaac tgaaagtaag tacggcagac ttattaagtt gcgtgcagtc
      421 ttttcacgtt atacaaacaa gaacacctgc gaagttattt ctccttacat atccccctat
      481 gccaacgggg gattttttct ggttcactaa cgcataaata atagtgacac cctatcattc
      541 acaaaccgtg ataatataag gattatcatc atggcaaaat taggtgaaaa caccccacat
      601 attatcgatg ctactgtaga ttttatggca tcaagccagg catttcgtga gtatctgaaa
      661 aaacttcccc ctgataacac aataccgcca gctcttactg atgagcacgt accgttatat
      721 atacgaaggc tggaatacta tcgtctgctt tatcgaccaa agcagacaga atcatattga
      781 aaaaaacggt gaacggttcg cgaaatcctt tcgcgggtcg tgaggcgttg gacaagcgaa
      841 gcgcgtcagt ttttccggct cttccccgtg acaggtttct ccaccagttt atcttccagt
      901 gtcacaactt tctgatacac cgccgctgtg gcgttgtagt tcctgtcgag tattgttttc
      961 attccccatc cgagcacaca gaacataacc agaaaagcca ctgacacccc acccagccat
     1021 ttccagctat accctttaag ggtgttgtat tcctgcacga tattgctgtg aattgtcgca
     1081 atatccctgt tcagggcatc cgcgtttgcc ttcatgtttg cgactgactc ttccagccgc
     1141 tgaaaatcct ggctgatgcg ctcacctatc agtcctgcca cgtccagctt ctgcacctca
     1201 tttctgactt catccctgac ctgttttgcc acaccgtcag aaatatcccg atatccgcga
     1261 agagtttccc ttgccacagc cagagaatca gcaacagctt tctgttgctc cattgcggta
     1321 tgttgcagat gctccaccga taacaggagc gtgagtaatt tttcttctgt gctggccatg
     1381 ttcagaatcc catagaaaat ccacgtgacg gtgatttaac ccgttcaggt gcagttcggt
     1441 tctgtctgct cactctgtct ctcatgtcaa agccatgctc ccgcgcaaca tcatgttgca
     1501 gccttctgac cagttcatgt tgcttaaccg gagtgtccag ccattccctg atgtcatgcc
     1561 cgaccgttcg cgcctgttca agatggcggg aatagtccgg ttcagaccgt ttttgcatga
     1621 tgtcatgacg ctgggcatca tcaagccgtc cggcagcttc aaaacccaga tgcacttcgg
     1681 gacgtcgttc aatgccctgt tctttcagcg tccggcagtc aatgaagccc ctttcaaagc
     1741 catgctttct catatgttcg ttgtgcttca cctcccagcg ttttcgcaat tcccgcaact
     1801 gctctttttg ctgagtgaga gtcaggcttc cggaatcctt tttcgcacct ccccgctcgg
     1861 gatacctggc gttatagcgc cggaaatact gttccggtgt acgttcaatg ccatcattca
     1921 cacgctgact catcatgata tgcgcatgag gctgttcgcc gccgtcaatg ctcgctttcg
     1981 gattgtgaat ggcaaaactc caggcgtgcc gttcgccagc ttcctgacgt acaaaatcct
     2041 gcaccagttc aaggcgctgt tccggtgtca gttcacgggg taatgcaatt tcaagttccc
     2101 tataggtcga accgttcgca cgttcaaact ggtcagccgc ctgccagaaa tgggaaggat
     2161 tatccctggc ccattccggc atgtttccgt gtgcggaata ttccaaatcc tgacggtcgc
     2221 ggtatttctc tttgcgctcg atgtaatcgg cgtggtttgc agcctgcccc ttgccaccaa
     2281 atttaacgct gaggtgatag gttgccacgg tttcagccct ctgcaggagc gcactgtttc
     2341 tcgacgaagg agagaaacat ataagtgcgc ttccttctaa ttttacgaat tagcacggaa
     2401 gataccccct gcacccgccg accgcaagcg atatactgca cgaccacaac acaccgcaaa
     2461 aaaacaccag ggagcatcac gaacatgcgc atcagcaaca tcgaatggct gaagaaacgg
     2521 atcggattca tcaggaagct cggagaacag accgcacgac agagacagat tattgacctg
     2581 atcgacaacg aagccggact cacagaacag gaacgcaaac ttctccacgt actggcaaca
     2641 gcggaaaaaa acgacttaca ggcacaggag agcgaacgaa aacaggcggt ccaaaaacga
     2701 atcgagggaa aaaaacaacg acgggaacgc aatcaccggc tctttctcgc ggctgggcta
     2761 ctgatcgaag caggactggt agacacaaaa acaggcgagc tatgctataa aaaagacagg
     2821 atattacaag cacttaagga acttaagtac gatctggaga catccccaaa cccagacgcg
     2881 taagtgcctc catccgtgtt ccgattccct ggtgcttcct cgctcattcg ctcgctacgc
     2941 tcggtcatga gactgcggcg agcactacca ccacaatcac gcggaaagcc gcgccattgc
     3001 agggctgaac ggggaaaaac ggcaaaaaac ctgctgctca aaaagacgcc agaaaggcgc
     3061 tgttatctga tataggctta tttggtctac tctacgagac ctcgtttttt cagttggtaa
     3121 taccaggcgc gactgatccc taatgcctcc cacggtttca gcgtgcgtgc agatgtagct
     3181 actgtgctgc gcttagactt agaaccgccc ttgcgaccgc gtgcagcctg aatttctggc
     3241 gtgtgcgtat cagccacata ctgtgcgaaa gtttccggcg tgaagttcct gtgcgtgtat
     3301 ttcgcaatgc tcttgccaat agcccgacat tcagccagtg aaagcggaac gggaagcgat
     3361 gcattgtaca tttcgacacg ctggatcacc gcttcaagcc actgtgagaa cgctggccag
     3421 ccctgacgaa tcgccctgta agcccatttg cgcgtcattt cgaacaggtg gcagtttcgc
     3481 cccatcccgt aatgtttatc gatgctacga cgctctgagg cgctcaaatc gagataatca
     3541 gccagttcat cgagggtata ggcttcctcg cgccactcca tcaccagcca ttccaggtgg
     3601 aacggatttt tgcaaatcag gccgctgtag ttcacatccg cacaaagttt ctcacacagc
     3661 gaacgttcaa ccgctgcggc atatttcagc gccttaaccg aggcatcagg cgcggttctc
     3721 acggcgatgt tgagtgcgta gagcaggtga gcgtgtccgt tcacaggatt tttaacggtg
     3781 atgttcggcg cgggagcgtt ccggtcgctc cagtcaatcg cagccccctc gcggtcaacg
     3841 tcaaaaacca gccagaactg tgcatgaggc tggttctgct ggatataccg tgcaagaagc
     3901 gcgcgccctt tgccggagat acgaaggcca aaagcgagat catccgtgtg atagggacgg
     3961 tggggtaaat tttcttcgaa gtattgaagc gcggcgctca tgtgttacga ttccttccag
     4021 gttatcgacc gacatcgccc ctgtattttc gtgaaagtct tggcggatag acacgaattt
     4081 caggggcgtt tgtttttcag tcgcttacac tacacccaac ggcaaatcct gtcgaacctt
     4141 cccgaaaacc cgatccattt accataattc ccgttgtacg ccccccttaa aacgccctca
     4201 gtcgctttct ggcgcaaaaa acgcaaaaag acaagaaaac accaggccaa accagaaaaa
     4261 cgcgtcagaa tcgttttcag ggcgttgcat aactatgcac gaaagcgatg tgcgatcggt
     4321 ttcgtgcgtt ctgaacaccg aacggctcaa aacgacaagt tttctcccct ggtgcgtggc
     4381 gctctgacgg ggtttcctgt ttccaaactt cacatctcgc caggacaaaa aaaaccgacc
     4441 tggggtcggt ggcaagaatc agagtctatg ggaaatattt tagttatcgg atgctcaata
     4501 ttcacaaacg caaaaaatgc ctcagtgatg ccctaaaaca acctttccag aaggagtccg
     4561 cgctttacga tgcacggaag agataaaaaa ccccacaaaa gtggggaacc aagagaaaca
     4621 acaattacag gttgttggtt gtgttcgaac aacgatgaag tagctacgct aaataataat
     4681 taccgtacag gccctacata ttcaacacta aatttaagat cctgacgaag gatttataaa
     4741 tgcacgaaaa cacatagaca gacaaattca ctaaattcat acacaaaaac aagtgtagtt
     4801 ttctaagtaa attagattcg acaaccaatt agtttatcca catcgcattc cactcttgct
     4861 acaccaatca catttcttga tatgtaatag cagaaaaaat aaaaccagac accaatagac
     4921 caaaaaacaa acattcaaca agaaacattt tgaacaatat cacaaaactg ccacaaccat
     4981 catcagacta ttgaaaaaaa ctggttgctg ttttataaaa gcgactgtaa caacctccac
     5041 tctatagttc cattagttta aagtgtcaca caaactgagc atccatttgt tttccttact
     5101 tttgcccctg tattccaagg ggttttttta taacttaaag attagcacac cacacaaaaa
     5161 caat
//
LOCUS       DAJVQH010000196         5019 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJVQH010000196 DAJVQH010000000
VERSION     DAJVQH010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.198"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            95..1210
                     /gene="adrA"
                     /locus_tag="OQM87_003947"
                     /gene_synonym="dgcC"
     CDS             95..1210
                     /gene="adrA"
                     /locus_tag="OQM87_003947"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCS1509877.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1227..2036)
                     /gene="proC"
                     /locus_tag="OQM87_003948"
     CDS             complement(1227..2036)
                     /gene="proC"
                     /locus_tag="OQM87_003948"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCS1509878.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2156..2614
                     /locus_tag="OQM87_003949"
     CDS             2156..2614
                     /locus_tag="OQM87_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCS1509879.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2797..3321
                     /gene="aroL"
                     /locus_tag="OQM87_003950"
     CDS             2797..3321
                     /gene="aroL"
                     /locus_tag="OQM87_003950"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCS1509880.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3371..3562
                     /gene="yaiA"
                     /locus_tag="OQM87_003951"
     CDS             3371..3562
                     /gene="yaiA"
                     /locus_tag="OQM87_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCS1509881.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3820..4497
                     /locus_tag="OQM87_003952"
     CDS             3820..4497
                     /locus_tag="OQM87_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCS1509882.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLEKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4569..4853
                     /gene="ppnP"
                     /locus_tag="OQM87_003953"
     CDS             4569..4853
                     /gene="ppnP"
                     /locus_tag="OQM87_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCS1509883.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT         1221 a         1192 c         1321 g         1285 t
ORIGIN      
        1 tagtgctaac gggtgagcta cgaaagtggc tcacccgaaa tttcatactt ctgcctttag
       61 ccccgtctct ataatttggg aaaattgttt ctgagtgttc ccaaaaataa tgaatgatga
      121 aaactttttc aaaaaagcgg cggcgcatgg ggaggaacct cctttaactc ctcaaaacga
      181 acatcagcgg tccgggctgc gcttcgcccg tcgcgtcaga ctaccccgtg cggttggcct
      241 ggctggcatg ttcttaccga ttgcttcaac gctggtttcg cacccgccgc cgggctggtg
      301 gtggctggtg ttggtcggct gggcgttcgt ctggccgcat ttagcctggc agatagcgag
      361 cagggccgtc gatccgctta gccgggaaat ttacaactta aaaaccgatg cagtattagc
      421 gggaatgtgg gtaggcgtaa tgggcgtaaa cgtgctgcct tccaccgcta tgttgatgat
      481 tctgtgtctg aatttgatgg gggcaggcgg cccccgtctg tttgtcgcgg gtctggtgtt
      541 gatggtggtt tcctgccttg tcaccctcga gcttacgggc attaccgtgt cgttcaatag
      601 tgcgccgctg gaatggtggc tctcccttcc cattattgtc gtttatcctc tgctgtttgg
      661 ctgggtcagc taccagacgg caaccaaact ggcggaacat aaacgcaggt tgcaggtcat
      721 gagtactcgc gacggcatga cgggcgtgta taaccgacgt cattgggaaa ctatgttacg
      781 caatgaattt gataactgtc ggcggcataa tcgcgatgca acgttactga ttatcgatat
      841 cgaccatttc aagagcatca acgatacctg gggtcatgat gtgggcgatg aagcgattgt
      901 ggcgcttacc cgacagttac aaattaccct gcgcggtagc gatgtgattg gtcggtttgg
      961 cggcgatgag tttgcagtaa tcatgtccgg tacgccagct gagagcgcca ttaccgccat
     1021 gttacgggtg cgtgaagggc taaacacgtt acgcttgccg aatacgccac aggtaacttt
     1081 acggattagt gtgggggttg cgccgctgaa cccacaaatg agtcactatc gtgagtggtt
     1141 gaaatcggca gatttggcgc tttacaaagc aaagaaagcc ggacgtaacc gcaccgaagt
     1201 ggcggcctga cgtccggcga aagtcatcag gatttgctga gtttttctga tttttccata
     1261 cacttcgtca tcgcttcgat cactgcggca cggaagcctt tctcttccag tacgcgtacc
     1321 gcttcaatgg tggtacctcc cggtgagcag accatatctt tcagtgcccc cggatgttct
     1381 cccgtttcca gcaccatttt tgcggaaccc attaccgcct gagcggcaaa tttatacgcc
     1441 tggacgcgtg gcatcccgcc cagcacggcg gcgtcggcca tcgcttcgat aaacataaat
     1501 acgtaggctg gcgaagaacc gctcacaccg accaccgggt ggatcatcgg ctcagcaatt
     1561 acttccgctt cgccaaagca gcggaaaata ttcagcacat cagcggtatc ttctggggtt
     1621 accagcgcgt ttggcgttac ggaggtcatc ccggcattaa ccagcgcggg agtgttcggc
     1681 atggcgcgga taattttccg gtcatggccc agcgcgcggg caagctggtc gagcgtgaca
     1741 cctgcagcaa tagaaacgac cagagagtct ttattcaggc tggaggtgat ttcgctaagc
     1801 actttaatca tgatgccagg tttaacggca gcaaaaatga tgtcggcgat ttgcgccact
     1861 tcttgcgccg attctgcggc gttgatgccg aactggtcat gcagggcggc gactttatcc
     1921 ggggaggggg tgtataccca gatttgccct ggaagcacct gaccgctggc aattagaccg
     1981 ccgagaatgg cttttcccat attgccgcag ccaataaaac cgattttctt ttccattgcc
     2041 tcactcctgc cgtgaaattc attgttttga taatcgctgg cagaagcata aacagaacta
     2101 tgccggaagg caaaagcgcg acacaataga ggattaccca acaaaggatg actttatgac
     2161 aatttgggtg gatgccgacg cgtgtcccaa tgtaattaaa gagattttgt atcgcgcggc
     2221 ggaacgtatg cagatgccgc tggtactggt agcaaaccag agtttacgcg tgccgccatc
     2281 gcgatttatt cgtacgctgc gcgtcgcggc aggtttcgac gttgccgata acgaaattgt
     2341 ccggcagtgt gaagcgggcg atttggtgat caccgcagat atacctttgg ctgctgaagc
     2401 catcgagaaa ggcgcagcgg cgcttaatcc gcgcggcgaa cgttacacgc cagcgaccat
     2461 tcgtgagcgc ctgacgatgc gcgattttat ggatacctta cgtgccagtg ggatccagac
     2521 cggcggacca gatagccttt cacaacgtga ccgccaggcc tttgccgcgg agctggagaa
     2581 gtggtggctg gaagtgcaac gtagtcgtgg ctaaatgtaa tttattattt acacttcatt
     2641 cttgaatatt tattggtata gtaagggatg tattgagatt ttcactttaa gtggaatttt
     2701 ttctttacaa tcgaaattgt actagtttga tggtatgatc gctattctca tgacaccggc
     2761 tttcgccgca ttgcgaccta ttggggaaaa cccacgatga cacaacctct ttttctgatc
     2821 gggcctcggg gctgtggtaa aacaacggtc ggaatggccc ttgccgattc gcttaaccgt
     2881 cggtttgtcg ataccgatca gtggttgcaa tcacagctca atatgacggt cgcggagatc
     2941 gtcgaaaggg aagagtgggc gggatttcgc gccagagaaa cggcggcgct ggaagcggta
     3001 actgcgccat ccaccgttat cgctacaggc ggcggcatta ttctgacgga atttaatcgt
     3061 cacttcatgc aaaataacgg gatcgtggtt tatttgtgtg cgccagtatc agtcctggtt
     3121 aaccgactgc aagctgcacc ggaagaagat ttacggccaa ccttaacggg aaaaccgctg
     3181 agcgaagaag ttcaggaagt gctggaagaa cgcgatgcgc tatatcgcga agttgcgcat
     3241 attatcatcg acgcaacaaa cgaacccaac caggtgattt ctgaaattcg tagcgccctg
     3301 gcacagacga tcaattgttg attttagagc gcctatactt aacgttcatc ccgtgaaata
     3361 aggaagaacg atgccaacga aaccgcctta tcctcgtgaa gcatatatag tgacgattga
     3421 aaaaggaaag ccaggacaga cggtaacctg gtaccaactc agagccgatc atcctaaacc
     3481 agactcgttg atcagtgaac atccgacggc tcaggaagcg atggatgcga aaaaacgcta
     3541 tgaggaccct gacaaagagt gaccgcatca gactgctcgg aagggattct gagtgccact
     3601 acaagggatc tgcgtcacat ttttcataat tcatgttttt ctaataatta gaatattaaa
     3661 caataacatt ccattactgg aatcatttgg aatctttaca ttatgccgtg cacgtctgct
     3721 gctacgcttt ttgtcatttg tagcacaagt aagtgtcagc agtggtgctt cacacttgcc
     3781 cggtaattaa cgacgaaaga aaagtaaggt ggatgaacaa tgagtgcgtc gttggcgatc
     3841 ctgaccatcg gcattgtacc tatgcaggag gttttgccgc tcctgacgga atacattgac
     3901 gaagataata tttcccatca tagcctgctg gagaagttaa gtcgtgaaga agtgatggcg
     3961 gagtacgcgc cagaagcagg cgaagacacc attctcacat tattaaatga caaccagctg
     4021 gcccatgttt cgcgtcgtaa agtggagcga gatctgcaag gtgtggttga agtgctcgat
     4081 aatcagggtt atgacgtcat tttattaatg agtacagcaa acattagtag tatgactgcg
     4141 cgtaatacga tctttcttga gccgtcgcga atattgcctc cactggtttc ctctattgtt
     4201 gaagatcatc aggtgggggt tatcgttccg gttgaggaga tgctgcccgt tcaggcgcaa
     4261 aaatggcaaa ttttgcagaa atcgccggta ttttcattgg gtaaccccat tcatgattca
     4321 gaacaaaaaa tcattgatgc tgggaaagaa ttactggcaa aaggggctga tgtcatcatg
     4381 ctggattgtt taggattcca ccaacgtcat cgcgatttac tgcaaaaaca gctcgatgtt
     4441 cctgtcttgc tctctaacgt attgattgca cggctggctg cggaattact ggtgtaattt
     4501 tgcgtgacag ccagcgcctc tggcccctat agtgaagtag atgttcaact accagacagg
     4561 gccagtttat gcttcaaagt aatgagtact tttccggcaa agtgaaatcg atcggctttt
     4621 ccagcagcag cactggtcgc gccagcgtgg gtgttatggt tgaaggcgaa tacaccttca
     4681 gcaccgctga gccggaagag atgacggtaa tcagtggcgc gctgaatgtg ttactgcctg
     4741 acgcgaccga ctggcaagtg tatgaagccg gttcggtgtt taatgttccc ggtcacagtg
     4801 agtttcatct tcaagttgcc gaacctacct cttatctgtg ccgctatctg taattcctcg
     4861 ccttcccctt gaacgggagg gcatttttct gaaatatcct ctctttaacc cataataata
     4921 tttcctttgc tgcgattttt tcaatttccg atatattcat aattcatcaa ggttgatata
     4981 aatatcagtg aagatctcca gggtaatgac tccaactta
//
LOCUS       DAJVQH010000197         4860 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJVQH010000197 DAJVQH010000000
VERSION     DAJVQH010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.199"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..181)
                     /locus_tag="OQM87_003954"
     CDS             complement(<1..181)
                     /locus_tag="OQM87_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCS1509884.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGL"
     gene            complement(245..2074)
                     /gene="recQ"
                     /locus_tag="OQM87_003955"
     CDS             complement(245..2074)
                     /gene="recQ"
                     /locus_tag="OQM87_003955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS1509885.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLLDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(2207..3076)
                     /gene="pldA"
                     /locus_tag="OQM87_003956"
     CDS             complement(2207..3076)
                     /gene="pldA"
                     /locus_tag="OQM87_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCS1509886.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3241..3708
                     /locus_tag="OQM87_003957"
     CDS             3241..3708
                     /locus_tag="OQM87_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCS1509887.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3760..4668
                     /gene="rarD"
                     /locus_tag="OQM87_003958"
     CDS             3760..4668
                     /gene="rarD"
                     /locus_tag="OQM87_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCS1509888.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSKSKINIF"
BASE COUNT         1152 a         1262 c         1221 g         1225 t
ORIGIN      
        1 gcaggccaag caacgcaatc ccagcccaaa ccattacgcc gcaggtaatg cccagcacgc
       61 ccatcatcgc ttctttacgg gaacgactga cagcggtctg agagacaaaa aagaaatcgg
      121 gaccggggct cataagcgcc acaatgtgca ccatggcgac ggtgagaaat aacatcaaca
      181 tacatttgac tcgcggggga aacgtttacg gagtcttcat actggcactt ttttatgctg
      241 ctgactactc ttcgtcgtcg ccatcgacat gcgcacgaat cagcgccata aacggtttac
      301 caaagcgttc cagcttgcgc atcccaacgc cgttaacgct gagcatttcg ctggcggtga
      361 tcggcatctg ttcagccatc tcaatcaagg ttgcgtcgtt aaacaccacg tacggcggga
      421 cattactttc atcggcaatc gatttacgca gtttgcgtaa tttggcgaac agtttgcgat
      481 catagttgcc gccgaacgat ttctgcatcg ctttcggttt gagcgccacg atacgcggca
      541 cggcaagttg caaagaggat tcgccgcgca gcaccgggcg cgcggcctct gtcagttgta
      601 gggcagaatg ctgggcaata ttttgcgtca ccaggccgag atgaatcagc tggcggatca
      661 cgctcaccca atgttcatgg cttttatcac ggcccatacc atagactttc agtttgtcat
      721 gaccatagtc gcggatacgc tggttattag caccacgaat cacttccacc acataaccca
      781 tcccaaaccg ctgattcaca cgaccaatgg tggaaagggc aatctgagca tcggttgaac
      841 cgtcgtactg tttcggcgga tcgaggcaga tatcgcagtt cccgcacggc tcctgacgcc
      901 cttcgccaaa atagttcagc aataccagac gacggcaagt ttgcgcttcg gcaaacgcgc
      961 ccatcgcatt gagtttgtgg cgctcgatat ccagcaactg cccctgcggc ttctcttcca
     1021 gacaacggcg cagccacgcc atatcagccg gatcgtaaaa cagcatcgct tccgcaggca
     1081 ggccatcacg cccggcgcga ccggtttcct gataatagga ttcgatattg cgcggaatat
     1141 caaagtggac cacgaagcga acgtttggtt tattgatgcc catgccgaac gccaccgtcg
     1201 ccaccacaat ttgcagatca tcgcgctgga atttctcctg cacgtcggcg cggacattat
     1261 tttccagccc ggcatgatag gccgccgcgc taataccctt gctttgcagg cgcgcagcgg
     1321 tgtcttctac tttcgcccgg ctgttgcagt agataatgcc agacttaccg cgctgttcct
     1381 gcacgtagcg catcaactga tcgagcggtt tgaatttctc catcagcatg tagcgaatat
     1441 tcggacggtc aaaactgctg atttgaatca gcggatcgtt cagccccagc aggcgcacga
     1501 tatcctggcg cgtggtgtcg tcggctgtgg cggtcagcgc cataaacggc agcgtcggga
     1561 accgctggcg caactgaccg agcgcggcat attccgggcg gaaatcgtgg ccccattggg
     1621 agatacagtg cgcttcatcg acggctaata acaccggatt ccagtgcgcc agatgctcaa
     1681 gaaagttatc cagcatcagg cgttccgggg cgatatacag taagcgaatt tgcccggtgc
     1741 ggcagcctgt catcacttca agttgctgtt cgcgggtttg cgtcgagtta aggcacgccg
     1801 ccgccacgcc gttggcctgt agttgatcca cctgatcttt catcaacgaa atcagcggtg
     1861 aaacaaccac ggtaaggccg tttagcaata aggcaggaat ttgatagcaa agggattttc
     1921 cgccaccagt gggcataaca accaggcaat cgcgaccgga aagcacagtg tcgataattt
     1981 cttcctggcc ggggcgaaac tgttggtagc caaaggtttc ttgtaaaacc tgtttagctc
     2041 cggactccag attcaacact tccgcctgcg ccacattcac cccgattgcc tgaaataaaa
     2101 acaggcgcta ttttcagcgc ctgcgcgaga aactgcaatg cttttagtct ctgagagggt
     2161 aatcctggat gctggattcc agatttgcca accactcaac cgttcatcaa aacaagtcgt
     2221 ttagcataac ccctacaccg acacgggtct ggttgaagtt atagtcgatg agcgattctc
     2281 catagccgct gtaaacctga gtataaaggc gcacatgttt ggtgatcggg taacttaagc
     2341 ctaactccgc gccgccgtag ccggtgttcc agttgtactg tcctttcgca ctgagcaccg
     2401 catcaccgag gtgatagccg attttaagct ggtagtaacc catatatttg gtgatatccg
     2461 ggttatcgtc agtattaccc accacatacc acggcttcac ttctaccagc cagttaccgt
     2521 tttctgccat caggcgagta taaaggcggt tccagctgcg ggaggtcggg tctgaacgcc
     2581 cgttagagtc gtggttatac cccatctcca catcgcgcag cgtccaacct gcaaaacggt
     2641 aatcggtggc aaaaccgagg aacaattgcg gttcgtagtt ggtttcacga aacggtgaag
     2701 actcttcgct attggacagt tgccaccagg atttttgcgt ataagacgca cccaacaccg
     2761 agtttgggcc taaaatccca cgccacagcg gaaatgccag gctcaactga aactttactt
     2821 catccttacg cgcattttcc gcccagtcgt aactggcaat cgcttcttta ttcagatcgc
     2881 tggtttgggt gtagatgagg tagttggtgt cataaggata gagcgtgaac ggattgtcat
     2941 gctcctgcag catattggcg ataatactgc cacgcaccgc tggcgcgtca tgcacctctt
     3001 tcaccgttgc ctcttgtgca tatactgcca taggcaacat aaacaccggc aacaaccagc
     3061 cctgcagagt ccgcatcggt cgtgttctcc gtaacgaatt attgataagg tgaatgtttt
     3121 tctgcgggct attttacata cttaactcca ttgccgttag ttatcccttc ctaaagtaga
     3181 aaccaacaac aaagaagcat aaaatcaaca tttcattaac caatgaagtg taaggatgat
     3241 atgtctgccg tactgaccgc tgaacaagcc ctgaaattag tgggtgagat gtttgtttat
     3301 cacatgccat ttaaccgcgc attggggatg gaactggagc gttacgaaaa agagttcgca
     3361 cagctggcct ttaaaaatca gccgatgatg gtgggtaact gggcgcaaag cattttgcac
     3421 ggcggagtca ttgcgtcggc gctggatgtc gccgccggtc tggtgtgcgt gggaagtacc
     3481 ttaacccgcc acgaaaccat cagtgaagat gaactacgcc agcggctatc gcggatgggg
     3541 accattgatc ttcgcgttga ttatctgcgc ccaggcagag gcgagcgttt tactgctacc
     3601 agtagcctgt tgcgtgcagg caataaagtc gccgtcgccc gcgttgaatt acacaatgaa
     3661 gaacagcttt atattgccag tgccaccgcc acctatatgg taggttgaag acataaaagc
     3721 aggtaaactg ctgttaccaa ttaaaaatga gtattcccga tggatgcaaa acaaacgcgg
     3781 cagggcgtat tactcgctct tgccgcttat tttatttggg gtattgcgcc agcatacttc
     3841 aagctgattt actacgtgcc cgccgatgag atcctgacgc atcgcgtgat ctggtcgttt
     3901 ttctttatgg tggtgctgat gagcatttgc cgccagtggt cctatttaaa aacgctgatt
     3961 cagacgccac agaaaatttt tatgctggca gtctctgccg tgctgattgg tggcaactgg
     4021 ctactgttta tctgggcggt gaacaatcac catatgctgg aagcgagcct tggttacttt
     4081 attaacccgc tggtgaacat tgtgctgggg atgattttcc tcggcgagcg attccgccgg
     4141 atgcaatggc tggcggtgat tctggcgata tgtggcgtgt tggtccagct gtggactttt
     4201 ggttcgctac ctattatcgc gctggggctg gcatttagtt ttgccttcta cggcctggtg
     4261 cgcaagaaga ttgcggtaga agcacaaacc ggcatgttaa ttgaaaccat gtggctgctg
     4321 cccgtggcgg cgatttacct gtttgctatt gccgacagtt caaccagcca tatggggcaa
     4381 aacccgatgt cgctgaattt actgctgatc gccgccggta ttgtcactac cgtaccgctg
     4441 ttgtgtttta ctgccgctgc cacgcgcctg cgtctctcaa cgttaggctt ttttcagtac
     4501 attggcccga cgctgatgtt cctgctggct gtgacgtttt atggtgaaaa accgggtgcc
     4561 gataagatgg tgactttcac ctttatttgg gtggcgctgg caatttttgt gatggacgcg
     4621 atttatacgc agcgtagaac gtccaaatca aaaataaata tattctgaaa aaatatgccg
     4681 actcttgtat gagagtgggc atattgccat gctgacagga gactcacaca aggatgtaaa
     4741 atttatgtta cgtatgttta tcccaacatc taacggtaag atttccagac gccgatatat
     4801 attttcattt attttaataa atttcatttt tgcctttctt attatctttt ttaacgatgg
//
LOCUS       DAJVQH010000198         4774 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJVQH010000198 DAJVQH010000000
VERSION     DAJVQH010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.200"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..188
                     /locus_tag="OQM87_003959"
     CDS             <1..188
                     /locus_tag="OQM87_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1509889.1"
                     /translation="GIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGGE
                     LETGKFQGTATITVMVR"
     gene            complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="OQM87_003960"
     CDS             complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="OQM87_003960"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCS1509890.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1156..3192
                     /gene="lpoA"
                     /locus_tag="OQM87_003961"
     CDS             1156..3192
                     /gene="lpoA"
                     /locus_tag="OQM87_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCS1509891.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLALQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLSVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCMINRKLSWLQYQQGQVVPAS"
     gene            3150..3545
                     /locus_tag="OQM87_003962"
     CDS             3150..3545
                     /locus_tag="OQM87_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCS1509892.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3565..4155
                     /gene="diaA"
                     /locus_tag="OQM87_003963"
     CDS             3565..4155
                     /gene="diaA"
                     /locus_tag="OQM87_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCS1509893.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4165..4740
                     /gene="yraP"
                     /locus_tag="OQM87_003964"
     CDS             4165..4740
                     /gene="yraP"
                     /locus_tag="OQM87_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCS1509894.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
BASE COUNT         1157 a         1253 c         1294 g         1070 t
ORIGIN      
        1 tcggtattcg tattgaaaat ctcggcggcg gagttgcaaa tattcctttt cagaacggta
       61 tccttcccgt tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc
      121 tggtcggtgg tgagctggaa acaggaaaat ttcagggcac agccaccatt accgtcatgg
      181 tgcggtaaaa gaagacgttg cgttttgatt aacgcaacgc ttcggcactg ttacccctgc
      241 tgctccagcg catacttata cagcgcattt ttcttcacgc catgaatttc tgcggccagc
      301 gccgccgctt ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg
      361 gcgggtaagt cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg
      421 cgacggtttt catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg
      481 gtttcccagg ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc
      541 gcaacgatat cttccaggct atctaacaga cggtgggtag attcataaaa aatcagcgtg
      601 cgcggctccg cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa
      661 aagccttcgt aacagaaacg gtcagatggt aaacccgcgg cgcttaacgc agtgatagca
      721 gcacacggtc cgggtagcgg caccacacga atccccgctt cacggcaggt acgcaccaga
      781 tggtagccag gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc
      841 ccctcttgca gcttcgccag cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc
      901 gcaaacaacc gggcattaat cccaaaatgt tgcagcaata aaccggtgtg acgagtatcc
      961 tcggcggcaa tcagatcaac ggcctgtaat acctctaacg tacgctgggt gatatccgcc
     1021 agattgccga ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg
     1081 tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat
     1141 atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg
     1201 cctgttgttc tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc
     1261 actgcttata tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag
     1321 caaagctctg atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa
     1381 gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat
     1441 tctcagcgtc gcgagaaaac actgctggcg gcagagatta aactggcgca gaaagatttt
     1501 gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa
     1561 gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg
     1621 cgcgcgttaa ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat
     1681 gccacctggc aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac
     1741 gccgacgaaa atattctgca aggctggctg gctctgcagc gcgtctggtt tgataaccgt
     1801 aacgatcccg acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaat
     1861 ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg
     1921 accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact
     1981 attcagcaag gctttgaagc cgcgaaaaat atcggcactc agccagtggc ggctcaggta
     2041 gctgccgcac ctgccgcaga cgtagcagaa caacctcagc cgcaaactgc ggatggcgtt
     2101 gccagcccgg cacaagcctc ggttagcgat ctgaccggtg atcagcctgc ggcccagccg
     2161 gtgcctgtaa gcgccccggc gacaagcacc gcagcggtaa gcgcacccgc aaatccatcc
     2221 gcagagctga aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt
     2281 cagcaggatg gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg
     2341 ctgaagagca acaccccgct gagcgtactg gcactgaacc agccggagaa tatcgaaaac
     2401 cgcgtcaata tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt
     2461 catattcgtg accagggtaa acaagcgccg ctggtgctga tcccacgcag tgcattgggc
     2521 gatcgcgtag ccaatgcgtt tgcgcaagag tggcagaaac tgggcggcgg caccgttctg
     2581 caacaaaaat ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatt
     2641 gctttaacgg gtagcccgat tactcccaga gcgacaaccg actccggcat gacgaccaac
     2701 aatccaacgc tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc
     2761 gatgcggtct acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc
     2821 atgcgtaacg gtagccagag cggtgcaacg ctgtacgcca gttcccgcag cgcacaaggc
     2881 accgcaggcc cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg
     2941 ctggcaggcg gtaatctgcc gttaatgcag caggcgctca gcgcggtgaa caacgattat
     3001 tcgctggctc gcatgtatgc gatgggcgtt gatgcctggt cgttggcaaa tcatttctca
     3061 caaatgcgcc aggttcaggg ttttgaaatc aacggtaata ccggaagtct gacggctaac
     3121 ccggattgca tgattaacag gaagttatca tggctacagt accaacaagg tcaggtagtc
     3181 cccgccagtt aaccaccaaa cagaccggcg atgcgtggga agcacaagcg cgtcgctggc
     3241 tggaaggcaa aggactgcgg tttatcgccg ctaacgtgaa cgagcgtggc ggcgagatcg
     3301 atctgataat gcgtgaaggc ctgaccaccg tttttgtcga ggtacgctat cgccgctctg
     3361 cgctttatgg cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga
     3421 ctgcccgctt gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg
     3481 atgtggtagc cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact
     3541 cataattaag gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc
     3601 attcaaactc aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcagccatg
     3661 acgctggttc agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc
     3721 gctgccaatg cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc
     3781 agcttacctg ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat
     3841 cgcttacatg atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta
     3901 ttgttagcca tttccacccg tggcaacagc cgcgatattg ttaaagcagt tgaagccgcc
     3961 gttacgcgtg atatgaccat tgtggcattg accggctatg acggcggcga acttgcaggt
     4021 ttgttagggc cacaggatgt ggagatccgc attccttcgc atcgtagtgc tcgcattcag
     4081 gaaatgcata tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct
     4141 caccaggatg attaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc
     4201 gcgctgctgt tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc
     4261 aaagccgcaa ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg
     4321 cgcgtgaaca gcgcattgtc gaaagacgaa cagattaaga aagaaacccg tattaatgtc
     4381 acggcctatc agggcaaagt gctgctggtt gggcagtcac caaatgctga actttcggct
     4441 cgcgccaaac agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt
     4501 cagggccagc cgattggtct gggcgaagca tctaacgata cgtggatcac caccaaagtg
     4561 cgttcgcagc tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa
     4621 aacggtgaag tgttcctgat ggggctggta actgaacgtg aagcgaaagc ggcggcagat
     4681 attgccagcc gggtgagcgg cgtaaagcgg gtaaccacag cgtttacgtt tattaaatag
     4741 cagccccttg taatgcctga tgcgacgctt gccg
//
LOCUS       DAJVQH010000199         4754 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJVQH010000199 DAJVQH010000000
VERSION     DAJVQH010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.201"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            278..808
                     /locus_tag="OQM87_003965"
     CDS             278..808
                     /locus_tag="OQM87_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCS1509895.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELISN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWCQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            815..1048
                     /locus_tag="OQM87_003966"
     CDS             815..1048
                     /locus_tag="OQM87_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCS1509896.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(1628..2746)
                     /locus_tag="OQM87_003967"
     CDS             complement(1628..2746)
                     /locus_tag="OQM87_003967"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1509897.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSNKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(2864..2953)
                     /locus_tag="OQM87_003968"
     CDS             complement(2864..2953)
                     /locus_tag="OQM87_003968"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509898.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(3389..3550)
                     /locus_tag="OQM87_003969"
     CDS             complement(3389..3550)
                     /locus_tag="OQM87_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCS1509899.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
     gene            complement(4202..4393)
                     /locus_tag="OQM87_003970"
     CDS             complement(4202..4393)
                     /locus_tag="OQM87_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1509900.1"
                     /translation="MTKQEKTALNMARFIRRQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            4457..4687
                     /locus_tag="OQM87_003971"
     CDS             4457..4687
                     /locus_tag="OQM87_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HCS1509901.1"
                     /translation="MLTIRVTDDEHVRLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGDELRRLRLAVREQGTRDDS"
BASE COUNT         1327 a          985 c         1199 g         1243 t
ORIGIN      
        1 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
       61 gcaggacgct gacactccct gataatctgc cggaaacgaa acaggcgatt gctgagggcg
      121 ttacacgcgg tttaccatgc cggagagata aaaggccgtc aggatgtgat tcaggcgctc
      181 acagaggcag ggattgacgt ggtcagggtg acgcgaagca gtatcagcat tgcagacccg
      241 aacggcggga agaatgtcag actgaaagga gcgttttatg agcaatcttt tacagacggg
      301 tgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa tgctgaacga
      361 cgagttcagg aagctcgaag aatctgtaaa cagggaactg atctcaaacg aatctttaat
      421 caggaacgct atcaacgacc atacatcagc cctgaaagaa cttctggaga ggtatcagaa
      481 aacgaccgtc gatacgatgg acgcacactg gaaaaccgta ctgaaaatga gcgtgaaacg
      541 ctggctgtgg ctgattatcg tctcagtact gatgtttgcc acgaccggga gcctgctgtg
      601 gtgccagggc atgaagataa acgcgaacat gaatattctc cgcgagcaga aagagagtct
      661 ggagaagctg aacgcgaaga catggggcgt gcgttatcac gaggacagca acggacgttt
      721 tctggtgttg cccgagggga tgaaagccga aacgaactgg acgatggaaa acgggaaaca
      781 gaatgccgta aggctggtac aggagtaaca gagcatgacg gagctggaaa gacatttgct
      841 gaacgcatta gagcaactgc aacaggacta tatgcagcgg ctgaacgaat gggagagcgc
      901 cttcgtggaa ttgcagaaaa tgttttcgct tacgcaacgg gacaacgcga tgctgaacga
      961 gcgggtcatg cagttgagtc agcaggtgca gcacttgagc gagcagacag aacgcttgag
     1021 ccagttatac agcgagaaca ggagataagg gacgaacggc tgatacagga atgcgaacat
     1081 gcgttatccc tggagcgtga gcgacagccg gaaatacagg aacggacact ggatggccct
     1141 tcgctgggat ggtaatcaga gacaggctaa aatgttgtca gcgcgtttcc ggtgcatttt
     1201 ttgggataat ggttggtttg gggggcaccg ggaacgcgct tcagtcagac cttgctgaaa
     1261 acataggaac gcgttaaaac gcatacgggc gcgattctgg tgtgtctttt gttgctgtgt
     1321 tttgcctcta ttgccagatc gcgctcattc ccctgaactt ctgttttccc gctcaggtcc
     1381 ccgtcttctt cgcttccgaa agcatgaaaa aaatccgttt cagctgtctc tggctggcaa
     1441 ttcctgcgtg attcacatgg ctgcatagct atgcatgtgg ttaaaattta ccgggtgcga
     1501 tcgcggcagt ttttcgggtg gtttgttgcc atttttacct gtctgctgcc gtgatcgcgc
     1561 cgaacgcgtt tcagcggtgc gtacaatgag atgttatggt aaattcaagt cttagatttt
     1621 atgatgttta attagctttt tttgcgttta taatgttcat cttttttatt aaaattgatt
     1681 caatctttga tgtgggtatt aatattttat ttgacaaata gaaagttaaa aaaaacacca
     1741 ttaatagtat aaataaatac ccaaaaacag aagcgttatt aatttcaatt attgatgtta
     1801 atctgtatat tactacatgc tgaatcaaca tggatatgta gatgcattta gcatatgagt
     1861 tgaaaaaata gaggttaaat ttcttattaa ttattaatgc tgctttgtaa atgataatgc
     1921 ttatgcacat taaaggtatt attgataact ggtgattgac tatgtttgtt ttgaactcat
     1981 taggccctat taataatact gtcaatatac atataacaac cgatagaata actgtggtgt
     2041 ttttttgtaa tttatttttg tatttaaatg caagcatccc aaccataaac tcaggaagtc
     2101 ggcaagcaaa aaaccaaaat gggaagttta aaaatgatga tgagatattt gatatcggga
     2161 atgttgtaat tgttatcaca aataaaaaaa atatgttacg tttaaatgta aaggtattca
     2221 taacgtgaaa aactatcggt gatatcacgt acagcataat aacgcaccca ataaaccact
     2281 cgcctgtaag gaaaagaaaa tcataagaga aatgggcgtt taatatacca tcaagggcaa
     2341 aaaatactgg aattattcta gtgtactggc ctcctgttgt cattattgat acgagaggga
     2401 ctgtattaat tatctcttta tttaaataag acaataaaat taagagtaaa gagataaaaa
     2461 agtaagtgat aatataagga gtaaggatcc ttactccttt gtcaaggaaa taatcaatgg
     2521 tattctttct atctagtgaa ataaatgcca aatatccaga tataaaaaaa aacaagctca
     2581 cacctacctg gccaagaacc cctccataaa atgatggtat gaaagatagc ctggtttggc
     2641 taaatatgta gccataatga gatataataa caaaaatgca ggctgttact cgcaatgtat
     2701 caattgacgg atctctatta gacataacct ttatttcgct gttcaaatta tcgcctttag
     2761 ttgatgatgt aaaattaacg ctataaataa gctacacatt ctagagcagc gaaaggatga
     2821 ttcaagtatt tttaataata aaacgcattg ataatgttta tgttcagatg gtgctgatag
     2881 ctttgtcatg ctgttccact gatgttgata acgacagaga cttttgtcct tttcttgtta
     2941 gatgctgcct cagattcgga ttatggcgtt aaaggggttt gacgctcaac cgaacggttt
     3001 cacacgttaa gagctttttg tctgataaat caaaatataa tggacattga aaaacgccct
     3061 caaaacggtg tctttttaat cgaagattgg cacaaaacgg acaccaccgt ccattagtcg
     3121 attttttaga cggcttttct cgcctaacgt gagttttcgt tcggttgagc atcagacccc
     3181 ttttatattg tagatttcta tttttagaca gttggaacag gacaattaac aacagatcta
     3241 cttgagatca tttgcttgtc gtcgtagtct ctttcccaga aacgaaaaaa ccgccctgca
     3301 aggcggtttc tcgaaggttc tcagagcgac caactctttg aaccaaggta actggcttat
     3361 gaggagcgca gtaaccaaat ctgtcctttc agtttagcct taacaggcgc ataacttcaa
     3421 gactaactcc tctaaatccg ttaccaatgg ctaccgccac tggcgatttg tcgtgtcttt
     3481 ccgggttgga ctcaagttga tagttaccgg aacgggcgtg gtagtcggac tgaacggggg
     3541 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
     3601 gaatgagata aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
     3661 ggagagcgca cgagggagcc accaggggga aacgcctggt atctttatag tcctgtcggg
     3721 tttcgccacc tctgacttga gcgtcggttt ttgtgatgct cgtcaggggg gcggagccta
     3781 tggaaaaacg ccatcggcgc ggccttcttc ctgtttctgg tcttttgctc acatgttctt
     3841 tccggcgtta tcccctgatt ctgtggataa cagcatttcg tttttgagtg agtagatacc
     3901 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc
     3961 ctggacgtgc attttctcct tacgcatctg tgcggcattt cacacccggt atggcgcact
     4021 tttcatacaa tctgcactga tgccgcatgg ttaagcccgt atacactccg ctatcgctac
     4081 gtgactgggt catggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg
     4141 cttgtctgct cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatat
     4201 gtcagaggtt ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg
     4261 cgtggtcgtg aagcgattca cagatatcag cctgttcatc cgcgtccagt tcgttgagct
     4321 tctccagcag cgttaatgtc tgccttctga taaagcgggc catgttaagg gcggtttttt
     4381 cctgtttagt cactgatacc tccgtgtaag ggggatttct gttcatgagg taatgatacc
     4441 gatgaaacgc gagaggatgc tcacgatacg ggttactgat gatgaacatg tccggttact
     4501 ggaacgctgt gagggtaaac agctggcggt atggatgcgg cgggtctgcc tgggtgagcc
     4561 ggttgcccgt tccgggaaac tgccgacact ggcaccgccg ttactgcgtc agctggctgc
     4621 catcggggac gagctgcgcc ggctgcgtct ggccgtcagg gaacagggga cgcgggatga
     4681 tagttaagtt tcatgcccgg ggaaaaggtg gtggcagtgg tccggttgat tacctgctgg
     4741 gaagggagcg taac
//
LOCUS       DAJVQH010000200         4706 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJVQH010000200 DAJVQH010000000
VERSION     DAJVQH010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.202"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..523
                     /locus_tag="OQM87_003972"
     CDS             <1..523
                     /locus_tag="OQM87_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-mannonate oxidoreductase"
                     /protein_id="HCS1509902.1"
                     /translation="CMEDEHYRHAAYGMMLQEQAPTLKVQGVDLQDYANRLIARYSNP
                     ALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAGWMRYVGGVDEQGNP
                     IEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNSLFTAKVTEAYLSLLA
                     HGAKATVAKYSVK"
     gene            complement(559..762)
                     /gene="ydfZ"
                     /locus_tag="OQM87_003973"
     CDS             complement(559..762)
                     /gene="ydfZ"
                     /locus_tag="OQM87_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCS1509903.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(940..1626)
                     /gene="ydfH"
                     /locus_tag="OQM87_003974"
     CDS             complement(940..1626)
                     /gene="ydfH"
                     /locus_tag="OQM87_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCS1509904.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSNWFSEE"
     gene            complement(1715..2461)
                     /gene="ydfG"
                     /locus_tag="OQM87_003975"
     CDS             complement(1715..2461)
                     /gene="ydfG"
                     /locus_tag="OQM87_003975"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCS1509905.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            2598..4643
                     /gene="dcp"
                     /locus_tag="OQM87_003976"
     CDS             2598..4643
                     /gene="dcp"
                     /locus_tag="OQM87_003976"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCS1509906.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1167 a         1127 c         1222 g         1190 t
ORIGIN      
        1 ctgtatggaa gatgaacatt atcgtcatgc ggcgtatggc atgatgttgc aggaacaagc
       61 gccgacgctg aaagtgcagg gcgttgattt gcaagattac gctaaccgat taattgcacg
      121 ctatagcaac ccggcgttac gtcatcgaac ctggcagatt gcgatggatg gcagccagaa
      181 attgccacag cggatgttgg attctgttcg ctggcatctg gcgcatgaca gcaagttcga
      241 tctgctggcg ctgggcgtcg cgggttggat gcgttatgtc ggtggtgttg atgaacaggg
      301 aaatccgata gaaatcagtg acccactgtt acctgttatt cagaaggctg tacaaagtag
      361 tgccgaaggg aaagcgcgcg tccagtcatt gctggcgatt aaggcaatct ttggtgatga
      421 tttgccagac aatagcttgt ttactgcaaa agtgacggaa gcgtacttgt ctttattagc
      481 gcatggcgcg aaagcgaccg tggcgaaata ttccgtgaag taaaaaaagc aatgccgcca
      541 tcggcggcat tcacacactt agttcatgcc gagacgaatc aggtccagag gtgccagttt
      601 ctcttcacaa ccttcaacaa ctacggtttt tccgcggcgg atttgctcag cggtcagacc
      661 ttcagtatca atcgacagga tcttgccagt gtgaccggtg ccgctaacca taacacggct
      721 gccagtggtg attgcgttac ggttacgatc gtaggtcgtc ataggtgttt tctcctttct
      781 gatttacagt cgaagcccac gtaacgggcg caatataaat actcctgtcg agtgaaatat
      841 tttttgtttt tgatcaagct cacatctttt ttgatgcatg catgagccgt aaggcggggg
      901 gaaaagcctc tcatcccgga tgggatgaga gtaaatgaat tactcttcgc taaaccagtt
      961 gctgttttcc tggcggatct ggcgcacgga ttcgctgatt tcctgcaaat gttgcgtcat
     1021 tgcacgttct accgcatcgc catcacgttt ttgcagggca gagaaaatat caagatgctg
     1081 gcgtaacagc atttctggtg gagaaacatg gtcgaaactc atatagcgca cgcgatcaac
     1141 ggtcgctttc aggttctcaa tggtatccca cgccagttga cagtcggcaa tctgcgtcag
     1201 gagttgatgg aagttgtcat caagttcaaa aaaatcatcc agttgcttgc gctcaatggc
     1261 aatgcgttgc tggtgaagat tttgttccag ttggtagcac tggctttcgg taatcatgct
     1321 cgccgcccga cgcgccaccg cgcactcaat ggcctgacgg ataaaactgc cgttgcgcac
     1381 ctgggccatg gaaattttgt tgacgtagct gccacgctgc ggacgaattt gaatcaggcc
     1441 gttttccgcc agtttaataa aggcttcacg aaccggctgg cgtgacacat tgaaacgaac
     1501 agaaacttct ttttccgaca acggtgtgcc tggtgcaatc aggcaatgga caatgtcgcg
     1561 acgaagaata cgataaatct gctgattgac aggctgtgtg ggattaagtt gcgtttcgac
     1621 ggtcattcgt tcttgcttgt gagtgagtta actgcatgag tctaccactt ttttgtgaca
     1681 agcaataacc cggcagttac gccgggtata aaaattactg acggtggaca ttcagtccgg
     1741 catagctttg ggtaaccggc atcatttcca gggtattgat attgacgtga gcaggcagcg
     1801 ttgacaccca ccagacggct tcgctgacat cttctggcgt caatgcaacg gtattttgat
     1861 aggttttttc cgctttaccg tcatcgcctt taaagcggac attggaaaac tcggtgccac
     1921 ccaccagacc cggttcgatg tcggtgacgc gcaccgccgt accatgcaga tccgtacgca
     1981 gattcaggct aaactgacga acaaacgctt tcgtcgcacc gtaaacgtta ccaccggcat
     2041 acggccagct gcctgccgtt gagccaatgt taataatatg accatgatta cgttcaacca
     2101 tacccggtaa gacggcgcgc gtcatatata ctaggccttt gttgttggta tcaatcatcg
     2161 tttcccagtc ttcaacgctg gctttatgcg caggctccat gcccaacgcc aggccggcat
     2221 tatttaccag gatatcaata ttgctccact cggcagggag cgatgccagc atctcttcaa
     2281 tagcggcgcg gttgcgaacg tccagttggg cgatatacag attatctccc agttcgtctt
     2341 ttaactcctg caaccgctcc tggcgacggc cagtggcgat aactttatgc ccttgttgaa
     2401 taaaacgacg agtaatgcat tcaccaaaac ctgccgttgc tccagttact aaaacgatca
     2461 tctcactgtt cctcaacgct tttgtgtgtc aataacatag cacgtgatgc ttaagagcgg
     2521 gtaatactgg tttgcaagat tgcactccgt tatcagtaag cctacgctat ggaacacctc
     2581 gcgttcagga gaagaaaatg acaacaatga atcctttcct tgtgcaaagc acactgccgt
     2641 atctggctcc tcattttgat caaattgcca atcatcacta tcgcccggca ttcgatgagg
     2701 gaatgcaaca aaagcgggca gaaattgctg ccatcgcgct taacccgcaa acgcctgatt
     2761 tcaacaatac tattctggca ctggaacaaa gcggagaatt aattacccgc gttaccagcg
     2821 tcttttttgc gatgactgcg gcgcatacca atgatgaatt acagcgtctt gatgaacagt
     2881 tttccgctga actggcggaa ctggctaatg atatctatct gaacggtgaa ttattcgcgc
     2941 gggtagatgc tgtctggcag cgccgtgaat ccctggggct tgatagtgaa tccatccgcc
     3001 tggtggaggt tattcatcaa cgttttgtcc ttgccggagc caaacttgca caagctgata
     3061 aagcaaaatt aaaagtactg aatacagaag ctgcgaccct gaccagccag tttaaccagc
     3121 ggttacttgc agcaaataaa tccggcggtc tggttgtgaa cgatatcgcg cagctggcag
     3181 gaatgagtga gcaagagatt gcgctggcgg cagaggcggc tcgcgagaaa ggtctggata
     3241 acaaatggct gattccgctg ctgaatacca cccaacaacc ggcgcttgcc gaaatgcgcg
     3301 atcgtgcgac gcgtgaaaaa ctgtttattg cgggctggac gcgagcggaa aaaaatgatg
     3361 ccaatgatac ccgcgctatc attcaacgtc tggtagagat tcgcgcacag caggcgaaac
     3421 tgcttggttt tcctcattat gccgcatgga aaatcgccga tcagatggca aaaacgccag
     3481 aagcagcact caactttatg cgggaaattg ttccagcggc gcgtcaacgt gcgagcgatg
     3541 aattagcctc catacaggcg gttatcgata agcagcaggg cgggtttagc gcgcagccgt
     3601 gggactgggc attttatgcg gaacaggtac ggcgggagaa atttgatctt gatgaggcgc
     3661 agctcaagcc atattttgaa ttaaacacgg tgttaaatga aggtgtattc tggaccgcga
     3721 atcagctctt cggtattaag tttgtcgaac gttttgatat tccggtctac catcctgacg
     3781 ttcgtgtgtg ggaaattttt gatcataatg gcgtgggact ggcgttattt tacggtgatt
     3841 tcttcgcccg tgattcaaaa agcggcggtg catggatggg caattttgtt gagcaatcaa
     3901 cgcttaatga aacgcatccg gtaatttata acgtctgcaa ttatcagaaa cccgctgccg
     3961 gtgagcctgc gttgttactc tgggatgatg taataacctt attccatgaa tttggtcata
     4021 cgctgcacgg cctttttgcc cgccagcgtt atgccacgct ttccggcacc aacacgccgc
     4081 gtgattttgt cgaatttccg tcgcaaatca acgaacactg ggcaacgcat ccgcaggtat
     4141 tcgctcgcta cgcccggcat tatcagagcg gggcagcaat gcctgacgaa ctgcaacaga
     4201 aaatgcgtaa tgccagcctg ttcaacaaag ggtatgagat gagcgaactg cttagcgccg
     4261 cacttctcga tatgcgctgg cattgcctgg aagaaaacga agcaatgcag gatgtcgatg
     4321 attttgaatt gcgggcgctg gtggcggaaa atatggatct tcctgctata ccgccacgct
     4381 atcgcagcag ttatttcgcc catatttttg gtggcggata tgctgcaggt tattacgctt
     4441 atctgtggac gcaaatgttg gccgatgatg gttaccagtg gtttgttgag cagggcggat
     4501 taacgcgtga aaatgggcag cgttttcgcg aggcgatcct ttccagaggt aacagcgaag
     4561 atctggaacg cctgcatcga caatggcgcg gtaaggctcc tcagattatg ccgatgctgc
     4621 aacatcgtgg cttgaacata taaacaccac caatggctgg cctggagata tcgccaggcc
     4681 agcctggtta tagcagtaat tgcagc
//
LOCUS       DAJVQH010000201         4663 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJVQH010000201 DAJVQH010000000
VERSION     DAJVQH010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.203"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(168..2009)
                     /locus_tag="OQM87_003977"
     CDS             complement(168..2009)
                     /locus_tag="OQM87_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCS1509907.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTAAYERALNNAANAIRPILSSQITAEQ"
     gene            complement(2009..3415)
                     /locus_tag="OQM87_003978"
     CDS             complement(2009..3415)
                     /locus_tag="OQM87_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCS1509908.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQENQAQRQKEFQQAYANAYASGDRGALRQLANQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLAASSPEAMQSWLQNNANELARVGVDPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            complement(3425..4117)
                     /locus_tag="OQM87_003979"
     CDS             complement(3425..4117)
                     /locus_tag="OQM87_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCS1509909.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            complement(4120..4575)
                     /locus_tag="OQM87_003980"
     CDS             complement(4120..4575)
                     /locus_tag="OQM87_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCS1509910.1"
                     /translation="MIQFKPTRNIDLIEAVGNHTDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(4575..>4663)
                     /locus_tag="OQM87_003981"
     CDS             complement(4575..>4663)
                     /locus_tag="OQM87_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509911.1"
                     /translation="LASNGSTLTIQSNGAAFTATTIKIIAEQ"
BASE COUNT         1058 a         1197 c         1054 g         1354 t
ORIGIN      
        1 gatacgatca cattggctgt aatgtctgtc atttaatttg ctccagatac aatgaatcgc
       61 cgcagcgtgg ctacggtgaa ttttgggcat aaaaaaaacc agccgaagct ggatagttgc
      121 gttggttatc tgtcagtagt tatgtgctta tttaattatt tatctatcta ttgttctgct
      181 gtaatttggc ttgaaagtat agggcgtatc gcatttgcag cattatttaa cgctctttca
      241 taagctgccg ttcctgcttt ggtgtttgcc aaacgaagaa gcatattcct tgcggctttg
      301 gactcataca aacgcatcat tgcaccaaaa ccagcctcaa gccccattga tacaccaaga
      361 gtcgcagttg cgccaatcgt ccttatcctg ttggcttgcg attgccccgt ctgagttact
      421 acatttgcgg tgtctgacct tgctgtttgc tgtagaactt catgaagagc atcaagctct
      481 ttcatgtgct ttccagaaaa aatagtgttg taaatttcac cgcctgactg agatttcagc
      541 ttattaactt cagtgatgaa cttggctgga gagtcaccgg ccttttccgc tattttgctg
      601 acgtaagctg cacgcatagc atctttccct ttatcatcca gtgcgctcca gattcgtttc
      661 acgtcagatg gttttctgct taatacaacg gtatttataa gttcaggact ggcttcactg
      721 cttgccttgt tgagcttgtt agcaatgttt ttattaagca ccttattata aacgtttgca
      781 taatcggaat ttgctttaag gtattttgct gcgtctgatg caccgaggtt tttagcaact
      841 gcgttacgaa ggtcttttga cattgcattc tctaccatat tggtagctgc ttttgcctgg
      901 ttggggaaga ccatagcatc tccctgaaca ttagatctaa atgctgttct gtgctgacgc
      961 aagagatcaa acgtaacatc caaatcagtt gcagggtttg ctaattcttc acgtaggtta
     1021 cgcaaggatg taagcaggct ttgattggca gacgtcccaa gccgttcctg tcttgcgatc
     1081 gctgtattca gagcattcat ggtatttgtg gtatcaactg cggcattacc cattttattg
     1141 gtgacgtcat tgataacagc gccagcggca tccttccgcc cccttaacgt ggtggtaaga
     1201 gatttcacca catcatcagg gttgtactca ccaaaacggt caaaataatt acttaccagc
     1261 ttactacgcg ttgcatattg ctccgctcgc tttgagcctg tcccgagcaa agccccctcg
     1321 gcatcctgag ttaggccgcg agtgaaagca tttttcggcg ggataacatc agatgtcatt
     1381 ggtgtcacgc ccatcgattc tgatgtggca attttcttcg ccacttctgg cgcaatatca
     1441 ccttttatag ccgttattcc acgccctatt ccctttgctg ctgcggaaag aacaccctga
     1501 gcggcaaggt taactccggc atttttagct gcattttgtg cgaaatcgcc tttctgattt
     1561 gcggcctctg ccagtgatcc aatagccatg cttcctgccg ttccaactcc tggaactaaa
     1621 tacccaccaa ttgtttcacc ggcttgcgcg taggggtctg tcggtctgtc tactggacga
     1681 taaacatcat ccaaaacctt gggtccacca agcccctgac tgattgcatt aatcagactt
     1741 gcgccgccct gcaatacgtc aaatggtatg tttaccagac cacgaccagc ctgttctgca
     1801 atttgccctg cactttgacc accagtgagc caatcgccag cttgttgcat caatgatggt
     1861 tcttcccgtg ttggtgcatt attggcctga ttaactgttt gttgctgaac agcctgacca
     1921 gcaaaatact catcaatggc ggtgccaata tcttcggtgc tcgtaccatc aggaaaggta
     1981 aatgtcttac cgtttgcagt tactttcatc attccaccgt aaattgaatg cctgattttg
     2041 aggtatatga tccaacctga ttccgtggtt ctcctgaagg tgtcgaatct tgtgctggcg
     2101 ctgcgtcagt attcattgac atataccgct taacggcact ccccaatgat tcaccttttt
     2161 taacatccaa ccccaatatc tgaccgccat tacgcgattg tccagggttg ccattcgcgc
     2221 tcatccactc ggctttaaac tcattaaact gcgcgtttcg tcgctcaagg tttgccattg
     2281 catcaagcca tcttgcgacc gtctcagggt tatccatgtc agttggcgca ccctgtcgaa
     2341 cgatctcaac gtctttatcc gttgctgggc cgggaggtag gaatttaaga acctgactgt
     2401 taacaagggc attttggcga atgcgcaaat cacgcaatgt tgtatcgctt ccggtaagtt
     2461 ttgcgaacat gttctgtgcg ttaccgaaca aacctgtcgt tggtttttct gctctgaact
     2521 gttgagcaag cgcactcata gaattggctg agtttgatga tgctgtagca ttgtttacag
     2581 ccgtctcgat gcctttttcc atgtttactg acagcttagg tgcttcgcta atcaactgct
     2641 gagccttttc ctgcgcttgc tgcatcttaa acccgaactc ttgctgatcc agagccaagc
     2701 gttgtgctgc gatattgtgc ccagtcattg ctgactgata ggaaaggttt tgccctctcg
     2761 cctgaagtgc ttctccagcc tgattgctgc ggattgtctc tgccagcctg cctcggtcaa
     2821 tttcacgacc agccatcttg tcctgaacat tgaagtaatc aatcggacca agagcagcca
     2881 ttccaaggtg atcaacaaac tcaccaaatc ctgaaggatt ctgctgatac atctgagcaa
     2941 cgctgttagg gtcaacaccg acgcgcgcca gctcattggc gttgttttgc agccatgatt
     3001 gcattgcttc tggagacgag gccgcaaggc gtgcgccagc cgctaaggtg ccgatagaat
     3061 tacgctggtc ttcatcaatg aatcccatgc ctttacgaac ggattcaatc tggtctggat
     3121 attgattagc caactgacgc aaagcaccgc gatcaccaga cgcataagca ttagcgtacg
     3181 cctgctgaaa ttctttctgc cgctgagcct gattttcctg ctgaaacacc cctgcaatac
     3241 ctgaaaggcc ttgcaaagca gtcagcccaa cattgttagc gcctgaacgc tcaatatcat
     3301 tgttctgcct gataagctga agcgtattgc cgatgtcatt tacgctcgga gcgtttgagt
     3361 tgacgccgcc gataccagct aacaatccgc catttgatcc ttgccaagta gccatgatta
     3421 ccccttaaaa caacgagcca agcaatccga taccagcacc aatgccagcg ccccaaggtg
     3481 ttgatgttcc caaaaggctg gcaagacctg caccggcaat cgcaccagat gttccgccac
     3541 taattgcagt ctgaagactt gatggtttat tggcattagc agcggcaaga gctgcgcttt
     3601 gctgtgcaat gctgctcatg ttgttggcgt acgtctgccc ggcgtttgcc tgaccttgca
     3661 gcgcaccaag cccaacgttt gccagattgt tgtaattgct catctggttt gataaccaag
     3721 actgaccgag tgtcggcgcg atcgtagcca gttgattgct tgtggctgtc gaaccaagtc
     3781 cacccgtcgc ctccgcagca gcaagactct ggtaacgagc ctgacctgca aggtctttat
     3841 actgctgaga gttgtaatac tgattaagtg cctgcccctg accttctaaa ctggaaaggt
     3901 tctgaagctg gttaacatac tgctccgcaa gaggcgtgaa cggagcaagg tttttcatga
     3961 tcgtctgcca ctgctgattt tgcaggtctg cggcatactt ctgagcttct gctgcatact
     4021 ttgcgctttt atcagagctg ccacctttcc cgcctttttc agggcaataa ggttcctcgc
     4081 cgcgcagttt tctgcccagc ttaaatgcat ataacatggc tatctcccgt gattcaggaa
     4141 gtcgattagt tcttcgcgtg tggcgctgta aaacgtcacg tcatccacgc ctttgaagta
     4201 tttcttgatg gttcctacac gcttaaggcc aatcattgcg cagtacatct gaccgtggcg
     4261 gaatttgcgc gcagcgaacg atgtgacgca ctgaacggtg gtgttagtca gaatgtatcg
     4321 ccagaacgcc agcccgattt ccttgctgaa tccacgaatc tctggcaggt acatggcgtg
     4381 gcaatcaaag gttagcggct gaatctcctg atagtaaaca attccgccga actgcccgtg
     4441 cacgttaacc tcaaagtaac ggcattcagg cttgtagtcg tatccatcgc cgttgttgct
     4501 cccggcgata atgtcagtgt gatttcctac tgcttcgatc aggtcgatgt ttcgcgttgg
     4561 tttgaactga atcatcactg ctccgcgatt atcttgatgg ttgtggcagt aaacgccgca
     4621 ccatttgact gaatagttaa cgtactgcca tttgaggcaa gaa
//
LOCUS       DAJVQH010000202         4579 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJVQH010000202 DAJVQH010000000
VERSION     DAJVQH010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.204"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..213
                     /locus_tag="OQM87_003982"
     CDS             <1..213
                     /locus_tag="OQM87_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1509912.1"
                     /translation="PQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEEL
                     SNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            356..577
                     /locus_tag="OQM87_003983"
                     /pseudo
     CDS             356..577
                     /locus_tag="OQM87_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(598..660)
                     /locus_tag="OQM87_003984"
                     /pseudo
     CDS             complement(598..660)
                     /locus_tag="OQM87_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1110..2522
                     /gene="ospC1"
                     /locus_tag="OQM87_003985"
     CDS             1110..2522
                     /gene="ospC1"
                     /locus_tag="OQM87_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCS1509913.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2626..2775)
                     /locus_tag="OQM87_003986"
                     /pseudo
     CDS             complement(2626..2775)
                     /locus_tag="OQM87_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2851..4548
                     /gene="ospD3"
                     /locus_tag="OQM87_003987"
     CDS             2851..4548
                     /gene="ospD3"
                     /locus_tag="OQM87_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCS1509914.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1604 a          815 c          851 g         1309 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatggggggc
      121 ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca gttacacagt
      181 tgtgaagccg gctctctcaa aaagggctta taaaggcttg agcggtgtac cgggaaaccg
      241 gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc cgacaaacat
      301 ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg aggaaatgga
      361 cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc aacaggacac
      421 tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg cttatgcttc
      481 cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt tcggtaaaag
      541 ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg tgatctacgc
      601 cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact cataagggaa
      661 gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc tctattaaaa
      721 aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga tttcattgca
      781 gaatacacat tccattaact ggaagcactc cataagagga atcagagaaa gtatcgtttt
      841 tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat tatgaaaata
      901 atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat tttgaaatat
      961 ctggcatcac ataacatcaa aagtatataa tacccttatc taattacatg cagtatattg
     1021 tatatatact caaaaattgt aattcatacc ctgctatata ttgtagggaa agatattaat
     1081 taaaactgtt ttcatataag gttcatttta tgaatatatc agaaacactg aactcagcaa
     1141 atacccaatg caatatagat tctatggata acagattaca tacattgttt ccaaaagtga
     1201 catcagtgcg aaacgctgca caacaaacta tgccagatga aaaaaattta aaagatagtg
     1261 caaatattat taaagatttc tttaggaaaa ctatagcagc acagagttat agtagaatgt
     1321 tctctcaagg ctctaacttt aaatctttaa atatagcaat tgatgcacca tcagacgcta
     1381 aagcctcatt taaggctatt gagcaccttg acagattatc gaagcattat atatctgaaa
     1441 taagggaaaa acttcatcct ctttctgcag aggaactcaa tttgctttcg ctaattatta
     1501 attctgattt aatcttcaga catcaaagta attctgattt gtctgataaa attttaaaca
     1561 ttaagtcatt caataaaatt cagtctgaag gaatatgcac aaaacgaaac acatacgctg
     1621 atgatataaa aaaaatagct aatcatgact ttgtgttttt tggcgttgaa atctctaacc
     1681 atcagaaaaa acaccccctg aatacaaaac atcacactgt tgattttggt gcaaatgcgt
     1741 atatcattga tcatgactct ccatatggat atatgacatt aaccgatcac tttgataatg
     1801 ctattccacc tgttttttac catgagcacc aatcattttt agataaattt tcagaggtta
     1861 ataaagaagt tagtcgatac gtacatggaa gtaaaggaat tatagatgta ccaatattca
     1921 atactaaaga tatgaagtta gggctcggat tatacctgat tgactttatt agaaaaagtg
     1981 aagaccaaag cttcaaggag ttttgctatg gaaaaaatct tgcccctgtg gatctggata
     2041 gaatcataaa ctttgttttt cagccagagt accatatacc taggatggta agtacagaaa
     2101 acttcaaaaa agttaagatt agagaaatat ccttagagga ggctgttaca gcatctaatt
     2161 acgaagaaat taacaagcag gtcactaaca aaaaaattgc tctccaggct ctttttcttt
     2221 cgattactaa tcaaaaagag gatgtcgcct tatatatatt atctaatttt gagataacta
     2281 gacaagatgt tatttccata aagcatgagt tgtatgatat tgagtatcta cttagcgctc
     2341 ataattcaag ctgtaaagta cttgagtatt ttatcaataa gggattggtt gatgtaaaca
     2401 caaagttcaa aaaaactaat agtggggatt gtatgttgga taacgcaata aaatatgaga
     2461 atgcagaaat gataaaacta ttattgaaat atggtgcaac atctgacaat aaatatattt
     2521 aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc acaaaatatc gatatattgt
     2581 ttattgtcag tatggctcaa tgtgataata gttggaaagt ttgatggggt tcgccccgtt
     2641 gtagcggtag tcgaactgga aggtcttcag gcactgctta cagcgataga gcagcccccc
     2701 tgaactggaa tggccgttcc ggataccccc ctgagtttca gagtaacggg gacaaaccac
     2761 atcaatcttt gccatcaatc atccaaaggg caaagagtac aacaacacta agtctgcgtc
     2821 acaacccatc aatgaaagga atatatacat atgccatcag taaatttaat cccatcaagg
     2881 aaaatatgtt tgcaaaatat gataaataaa gacaacgtct ctgttgagac aatccagtct
     2941 ctattgcact caaaacaatt gccatatttt tctgacaaga ggagtttttt attaaatcta
     3001 aattgccaag ttaccgatca ctctggaaga cttattgtct gtcgacattt agcttcctac
     3061 tggatagcac agtttaacaa aagtagtggt cacgtggatt atcatcactt tgcttttccg
     3121 gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa aggctattaa tgtgcctgct
     3181 attatttatt ttgttgaaaa cggttcatgg ggagatatta ttttttatat tttcaatgaa
     3241 atgatttttc attccgaaaa aagcagagca ctagaaataa gtacatcaaa tcacaatatg
     3301 gcattaggct tgaagattaa agaaactaaa aatggggggg attttgtcat tcagctttat
     3361 gatcccaacc atacagcaac tcatttacga gcagagttta acaaatttaa cttagctaaa
     3421 ataaaaaaac tgactgtaga taattttctt gatgaaaaac atcagaaatg ttatggtctt
     3481 atatccgacg gtatgtctat atttgtggac agacatactc caacaagcat gtcctccata
     3541 atcagatggc ctaataattt acttcacccc aaagttattt atcacgcgat gcgtatggga
     3601 ttgactgagc taatccaaaa agtaacaaga gtcgtacaac tatctgacct ttcagacaat
     3661 acgttagaat tacttttggc agccaaaaat gacgatggtt tttcaggatt gcttttagct
     3721 ttacaaaatg ggcattcaga tacaatctta gcatacggag aactcttgga aacttctgga
     3781 cttaaccttg ataaaacggt agaactacta actgcggaag gaatgggagg acgaatatcg
     3841 ggtttatccc aagcacttca aaatgggcat gcagaaacta tcaaaacata cggaaggctt
     3901 ctcaagaaga gagcaataaa tatcgaatac aataagctga aaaatttgct gaccgcttat
     3961 tattatgatg aagtacacag acagataccc ggactaatgt ttgctcttca aaatggacat
     4021 gcagatgcta tacgcgcata cggtgagctc attcttagcc cccctctcct caactcagag
     4081 gatattgtaa atttgctggc ctcaaggaga tatgacaatg ttcccggact tctgttagca
     4141 ttgaataatg gacaggctga tgcaatctta gcttatggtg atatcttgaa tgaggcaaaa
     4201 cttaacttgg ataaaaaagc agagctgtta gaagcgaaag attctaatgg tttatctgga
     4261 ttgtttgtag ccttgcataa tggatgtgta gaaacaatta ttgcttatgg gaaaatactt
     4321 cacactgcag accttactcc acatcaggca tcaaaattac tggcagcaga aggcccaaat
     4381 ggggtatctg gattaattat agcttttcaa aataggaatt ttgaggcaat aaaaacttat
     4441 atgggaataa taaaaaatga aaatattaca cctgaagaaa tagcagaaca cttggacaaa
     4501 aaaaatggaa gtgattttct agaaattatg aagaatataa aaagctgaat attatctcgt
     4561 ggtgatacac tgaatttga
//
LOCUS       DAJVQH010000203         4539 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJVQH010000203 DAJVQH010000000
VERSION     DAJVQH010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.205"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(346..528)
                     /locus_tag="OQM87_003988"
     CDS             complement(346..528)
                     /locus_tag="OQM87_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509915.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNSEDN
                     DEAASDYALKINESSD"
     gene            859..1731
                     /locus_tag="OQM87_003989"
     CDS             859..1731
                     /locus_tag="OQM87_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCS1509916.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHWGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1949..4333
                     /locus_tag="OQM87_003990"
     CDS             1949..4333
                     /locus_tag="OQM87_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCS1509917.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            4330..>4539
                     /locus_tag="OQM87_003991"
     CDS             4330..>4539
                     /locus_tag="OQM87_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCS1509918.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINAS"
BASE COUNT         1202 a         1090 c         1180 g         1067 t
ORIGIN      
        1 gctgctcacg gtgttgatcc gcgaactgag cgggagggga agatgatttc actcccatca
       61 ggtacccgta tctggctcgt tgccggtgtt accgatatgt gtaaatcttt caacggatac
      121 gacatatgct gaaggaggat ctgtaatgga ttaagtgata acagatactc agaaatatag
      181 gggcaaatcc actattttga tgagaattat tatatgttac ctcggatttt aatcaggcgc
      241 caagattaaa taaaatattg agcgcctgat tatatttatt cattcattca ttcattcatt
      301 aattaatatc tgttattgat tggcttacta tcagcttccc ccgtattaat cagagctttc
      361 atttattttt agtgcataat cagatgccgc ctcatcatta tcctcactat tatcatagcc
      421 gaaaacttcc cccggacacg ggcgttcacg ataatagtca tcgtcatagt tttcatcgtc
      481 agataatgat atgtcgtatt ctgaatatga ttcaccacaa ttatacatga aatcatctcc
      541 tttggggtgg ttacaataat tagtgagttt attcgaaaaa atgatgagcg ccagaccaac
      601 ccacccaact tttgattata atcatgaaga tacttgtctc agtacaaata gaatcaaact
      661 cagtgcagca tgattcacct ttatattctc tcagggaaac caaataccac tactcaacta
      721 ttaagaacaa aattctggag gtatgattat ctgaagatac ctgtatccta aataatttca
      781 gacagatatt atcgttgtga gtcccccgcc acctttccgg tgcgcggggt tttgtctttt
      841 ttcaccggga atacatgtat gaatccgtct gatgccattg aggcaattga aaaaccgctc
      901 tcctccctgc cttactcact ttcccgtcac atcctggaac atctgcgcaa actcaccagt
      961 cacgaacccg tgattggcat tatgggtaaa agcggggccg gtaaatcctc actctgtaat
     1021 gcactgtttc agggggaggt caccccggtc agtgatgttc acgccggcac ccgggaagta
     1081 cggcgcttcc gtctgagtgg ccatggtcac agcatggtta tcactgacct gcccggggtg
     1141 ggcgagagcc gggacaggga tgcagagtat gaggccctgt accgtgacat tctgcctgaa
     1201 ctggacctgg tactgtggct gattaaagcc gatgaccgtg ccctgtctgt ggatgagtat
     1261 ttctggcgac acatcctgca ctggggacat cagcgggtac tgtttgtggt gacgcaggcc
     1321 gacaaaacgg agccctgcca tgaatgggat atggccggta ttcagccttc tcctgcacag
     1381 gcacagaata ttcgcgaaaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg
     1441 gttgtggccg tatcggcccg cactggctgg gaactggata cgctggtcag tgcgctcatg
     1501 acagcgcttc ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgggctgcgc
     1561 acggaatctg tccggggtca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt
     1621 gacacggcgg agagcgtctg tgttgcctct gttgcccgta aggttctgcg cgctgttcgt
     1681 gacaccgtgg tctctgttgc ccgcgcggta tggaactgga ttttcttctg aacccggtgg
     1741 tgatgcacca gactcttgtt acagattttt aaccccatga aaacacaatg caaacacagc
     1801 catacccggg ttgtcccctt gcgttgtgtt tctcaagtta atagaatgca tcaatcgtta
     1861 ctatcatatc gatcgctata aacgatcgtt tgtggtaaat gacctcctcc gtggttgtca
     1921 tgaaaatgcg tctcaggaag gttaaataat gcacgaaaag aacatcgccc tgctttgtga
     1981 tgaagccgac cgacttttgc aactgaacat taatctgctc cggcaaatgg ttgatgaacc
     2041 ggatgtgtta tctgacagta agaacgaaaa cagactgctt tttgataaac agaaagcact
     2101 gaaaagaatt gaggagctgg agggcgaaca aatcaaaacc gcccgcaggg agatggtgct
     2161 ggctgttgtc ggcacgatga aagcaggtaa atcaactacc atcaacgcca ttgtggggca
     2221 ggaaattctg cctaaccgta accgccccat gacctctgta ccgacgctca tccgccacgt
     2281 tcccggaaaa actgagccgg tcctccatct ggaacatatt cagcccgtcc gcaatttatt
     2341 aagcacactg caacaaaaac tcgacacccc ggcaggacag caagtcgcac agaccctgca
     2401 gcaaaccggg gatacctgcg aactgctgga tattctggca aatgatggct ggctcaaaaa
     2461 tgaataccac ggggaagacg aaatctttac cggactggca tcgttaaacg atctggttcg
     2521 ccttgctgcg gcaatgggga gtgagtttcc ttttgatgaa tacgcagaag tgcagaaact
     2581 gccggtgatc gacgtggaat tcagccatct ggtggggatg gatgcatgcc agggaacact
     2641 cacactgctg gatacccccg gccctaatga ggccggacaa ccgcagatgg aagtgatgat
     2701 gcgggatcaa ctgcagaaag cctctgcggt gctggcggtg atggattaca cccagatgaa
     2761 ctcaaaagcg gatgaagacg tccgtaaaga gcttaatgcc attgctgacg tatcagccgg
     2821 ccgcctgttt gtgctggtca ataaatttga tgagaaagac cgcaatggcg atggggcaga
     2881 tgccgtacgc cagaaagttc cggcaatgct gaacagcgat gtgctgcccg cctcccgcgt
     2941 ttatccaggc tcatcacgtc aggcgtacct ggctaaccgt gcgcttcatg agttacggaa
     3001 aaacagaacc cttcctgttg atgaagcctg ggtcgatgat tttgtcaggg aagccttcgg
     3061 tcgcatgaaa aaagaatacg tctgtaaaga cagtgaaatg gcaaccgaag gggcgacaga
     3121 cctgtgggaa ggctcactta tcgatcaact gataacggaa gtcatacaga gctcccattc
     3181 cagagcggcg gcactggcgg ttgattctgc cgccgcaaaa ctgatgcaga atgcggaaaa
     3241 tgtcagcgaa tatctgtcat tacgccatca ggggctacag caaagcattc agtcactgca
     3301 gtcgcatatc accagcctgc ttgcggatat ccgggaaatc gcggactgtc aggagcaggt
     3361 gaccgctgat gtcagaatgg ccatggagga gatcgatgcc agaacccggg aattactgac
     3421 gggggtctgc acctcactgg aagaggagct gaatgactat ttcagaagcg gtaaacgcaa
     3481 agaacagcaa atgctggagg aagaaaattc agcgcagccc cgggagagaa acgcattcgc
     3541 tttctttcac gatattttcg gaaccggaaa tcagcatgac aggatgcggg actttgatcc
     3601 ggacagcccc gaaataaaat tcagtgatcg cagggaagcc cttgaactga tgacgcaaat
     3661 cgaatcgacc gtgaccagcc tgcaccgtga ggctgaagca cagttccgtc ctgaactgga
     3721 aaaaatcgtc aggggtattg aaacaggttt tcgtggtacg gccctgtacg ccacagaaaa
     3781 tattgccgga cgcatcaatg cccgtctgga ggatgaaggt ttcactgtaa aaatcagttt
     3841 tccggcagtc agccagttac agacccggct cgcagtaaaa acaaatctga gcgcgcttat
     3901 ggaggaaaga acggagacag taacccgtcg ctgtcgacag gatggtgtct ggggtacgct
     3961 ttgtcgatgg gcaaacacca gtgactgggg ctggaaagag tacagtgtgg atgtgagccg
     4021 cagcgtgatc aatatcaaca aggtcagaga ggaagtgatg tcactgaccc gggcatattt
     4081 cggggagctg caggcatcca ttgagcagga tattaaccag cccgtccgcc aggaaatcga
     4141 tgcctttttc tgtacattca gggagaaagt tgaacagctg cgtaacacgc tgattcagag
     4201 ctctgaagat cataaacgcg atcagcaggc gcaggaacgg ctgaccgggc gtcttcaggc
     4261 gttaaatgaa agggttcctg agctcattac tgacagtaag gcgttaaggg aagaactgga
     4321 gacaatgctg tgacttcacc atttattcaa caaattgctg ataaccgggt atgtcaggta
     4381 ctcacctgtc ttcctgaaaa atttgtggtt gatttcgcaa acggtattga tgttgcacag
     4441 gagcatatcc gtacggccgg agagcgcacg ttcttccggc ggttaaaaga ggggctgagc
     4501 ggcgaaggta ctgcacggca gaatgccatc aatgcttcg
//
LOCUS       DAJVQH010000204         4443 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJVQH010000204 DAJVQH010000000
VERSION     DAJVQH010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.206"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..436)
                     /locus_tag="OQM87_003992"
     CDS             complement(<1..436)
                     /locus_tag="OQM87_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCS1509919.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEE"
     gene            complement(551..626)
                     /locus_tag="OQM87_003993"
     tRNA            complement(551..626)
                     /locus_tag="OQM87_003993"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(591..593),aa:Thr,seq:ggt)
     gene            complement(633..707)
                     /locus_tag="OQM87_003994"
     tRNA            complement(633..707)
                     /locus_tag="OQM87_003994"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(672..674),aa:Gly,seq:tcc)
     gene            complement(824..908)
                     /locus_tag="OQM87_003995"
     tRNA            complement(824..908)
                     /locus_tag="OQM87_003995"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(872..874),aa:Tyr,seq:gta)
     gene            complement(917..992)
                     /locus_tag="OQM87_003996"
     tRNA            complement(917..992)
                     /locus_tag="OQM87_003996"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(957..959),aa:Thr,seq:tgt)
     gene            1354..2304
                     /gene="coaA"
                     /locus_tag="OQM87_003997"
                     /gene_synonym="panK"
     CDS             1354..2304
                     /gene="coaA"
                     /locus_tag="OQM87_003997"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCS1509920.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2333..3298)
                     /gene="birA"
                     /locus_tag="OQM87_003998"
     CDS             complement(2333..3298)
                     /gene="birA"
                     /locus_tag="OQM87_003998"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCS1509921.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3295..4323)
                     /gene="murB"
                     /locus_tag="OQM87_003999"
     CDS             complement(3295..4323)
                     /gene="murB"
                     /locus_tag="OQM87_003999"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCS1509922.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1137 a         1113 c         1004 g         1189 t
ORIGIN      
        1 gctcttcgtc atcaaccatg tcgcatttgt tcaggaacac gatgatgtac ggaacgccta
       61 cctgacgccc cagcaggatg tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa
      121 ctaccaggat cgcgccgtcc atctgagcag caccggtgat catgttttta acatagtcgg
      181 cgtgccccgg gcagtctacg tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag
      241 aagtgttgat ggtgatacca cgagcttttt cttccggcgc gttatcgatc tggtcgaatg
      301 cgcgagcagc accgccgtag gttttagcca gtacggtagt gattgcagcg gtcagcgttg
      361 ttttaccatg gtcaacgtgg ccgatagtac cgacgttaac gtgcggtttt gtacgttcaa
      421 acttttcttt agacatcgat tgtccctcta agacacggat aaatcggtga tatcaccaca
      481 tcaaccaggc aacatgcccg acttgttgaa tgcaataaac agaaggaaaa aacagggagg
      541 agaaaagaag tggtgctgat aggcagattc gaactgccga cctcaccctt accaagggta
      601 cgctctacca actgagctat atcagcacat cttggagcgg gcagcgggaa tcgaacccgc
      661 atcatcagct tggaaggctg aggtaatagc cattatacga tgcccgcatc ctagaactcg
      721 gctacctgat tttcattctg cactgaatat cgagagaagc tctctttatt cgagccggta
      781 agtgaactta tcgtctcggg ctacgccatc gcgtggccga aattggtggt gggggaagga
      841 ttcgaacctt cgaagtctgt gacggcagat ttacagtctg ctccctttgg ccgctcggga
      901 accccaccgg acttgatggt gccgactacc ggaatcgaac tggtgaccta ctgattacaa
      961 gtcagttgct ctacctactg agctaagtcg gcatcaagta gcgcgcattc tatggagaca
     1021 tgcgagttca tgcaactaaa aaattgcata atttgtttta ttggtcacat tttatgctac
     1081 acgatgaaga aacagccgaa cggagggcag attagcacac tttttcaaca tcattgtgct
     1141 caacaatgcg ctcctgctaa accataattc tttttatcag atggaatatc tgtcacattg
     1201 cttttcaacg atagcttcct ggcagagatt ttttcttatt attcctcccc atctggtgtt
     1261 accctcctgc ccattaaccc attcaacaga actgtgacgc gccatggcaa atatcgcttt
     1321 gccgatagag ctatgaccgc cagaaacatg cttatgagta taaaagagca aacgttaatg
     1381 acgccttacc tacagtttga ccgcaaccag tgggcagctc tgcgtgattc cgtacctatg
     1441 acgttatcgg aagatgagat cgcccgtctc aaaggtatta atgaagatct ctcgttagaa
     1501 gaagttgccg agatctattt acctttgtca cgtttgctga acttctatat aagctcgaat
     1561 ctgcgccgtc aggcagttct ggaacagttt cttggtacca acgggcaacg cattccttac
     1621 attatcagta ttgctggcag tgtcgcggtg gggaaaagta caaccgcccg tgtattgcag
     1681 gcgctattaa gccgttggcc ggaacatcgt cgtgttgaac tgatcactac agatggcttc
     1741 cttcacccta atcaggttct gaaagaacgt ggtctgatga agaagaaagg cttcccggaa
     1801 tcgtatgata tgcatcgcct ggtgaagttt gtttccgatc tcaaatccgg cgtgccaaac
     1861 gttacagccc ctgtttactc gcatcttatt tatgatgtga tcccggatgg agataaaact
     1921 gttgttcagc ctgatatttt aattcttgaa gggttaaatg tcttacagag cgggatggat
     1981 tatccacacg atccacatca tgtatttgtt tccgattttg tcgatttttc gatatatgtt
     2041 gatgcaccgg aagacttact tcagacgtgg tatatcaacc gttttctgaa attccgcgaa
     2101 ggggctttta cagacccgga ttcctatttt cataactacg cgaaattaac taaagaagaa
     2161 gcgattaaga ctgccatgac attgtggaaa gagatcaact ggctgaactt aaagcaaaat
     2221 attctaccta ctcgtgagcg cgccagttta atcctgacga aaagtgctaa tcatgtggta
     2281 gaagaggtca gactacgcaa ataatttgca ggggagcgaa tactcccctt tcttattttt
     2341 ctgcactacg cagggatatt tcaccgccca tccagggttt tattattcca tcctgctcaa
     2401 gtaataaagc cccctgtttg tctattccgc gtgaaatgcc aaatatttct ttatcaccaa
     2461 tgataagttt cactgggcga ttaataaaat tatccagctt ttcccagcgc gacagataag
     2521 gtgccaatcc ttcttgttcg aagagttcca acgcagcacg taattcacgt attagcatgg
     2581 ccgccaacgt attacgatca agattgatcc ccgcttcctg cagcgtgatc cacccctgat
     2641 taacgacact ctcttcaaca cggcgcattg ccatgttgat cccggatcca atgactattt
     2701 gcgccgcatc gccagttttg ccagtcagct ccacaagaat gcctgccagc ttgcgatcct
     2761 gcagatagag gtcattaggc catttgacac gaactttatc tgctcccagc tttcgtaata
     2821 cttccgccat cacgataccg ataaccagac ttaaaccaat cgccgccgcc gggccttgtt
     2881 ccagacgcca gaacatcgac aaatataagt ttgcgccaaa aggcgaaaac catttccgcc
     2941 cccggcgacc acggccagcc tgctggtatt ctgcaacaca ggcatcgccc gatttaagct
     3001 ctccgatacg atcaagaagg aactgattcg tggagtcaat aactggcagc acggctacac
     3061 taccgccatc cagctgaccc aatatctgtt cagcattaag taactggatg ggctcaggca
     3121 ggctgtatcc tttacccgga acggtaaaga catcaacgcc ccagtcacgc agtgtctgaa
     3181 tgtgtttatt aatagccgcc cggctcattc ccagcgtttc acccaactgc tcgccagagt
     3241 gaaattcacc gttcgctaac agggcaatca atttcagtgg cacggtgtta tccttcatga
     3301 aattgtctcc actgcgctca cttcacctga tgcaccaata aagcggactt caggctcaag
     3361 ccagacatta aatttttcac ccactttttg tcttacatgg tgtgccagtt gcaccacatc
     3421 ttcgcttttt gcattgtctt cattaatgag aactaacgcc tgttgacggt gcaccgcagc
     3481 cccacccatt tgcatccctt ttagctggca ctgatcaata agccaacctg ctgccagttt
     3541 tactgaacca cccgcctggg ggtaatttgg tgctgttgga aattgcgcca gtaatgcttt
     3601 agccgtttcg gcagatacaa cagggttttt gaagaaacta ccggcattgc cattcacttt
     3661 tggatcaggg agtttggtgg tgcgcatatg acacaccgca ttaaatactt gctgtggcgt
     3721 tactgttgta ggatccagac gagttaagtc accatacgtt agtacaggtt gccactcttt
     3781 tggcagacgc agacctacgg ctacaatggc gaagcgatcc tggtattcat gtttaaaaat
     3841 actgtcgcga tagccaaaac ggcactcttt ggcagttaag cgcacttgct tgcctgtcgc
     3901 cagttcaaca caatcaacat aagcgcaaac tcgctgtaat tctacgccat aagcaccaat
     3961 attctggata ggtgatgagc cgacacaacc aggaattaat gccagatttt ccagaccagg
     4021 cataccttcc tgcaaagtgt attttaccag gcgatgccag ttttctccgg ctcctacatg
     4081 taaataccac gcatcaggtt catcatgaat ttcgatacct ttgatccggt tgatgatcac
     4141 cgtgccgcga tagtcttcca gaaaaagtac attacttcct tcacccagaa taagaacggg
     4201 ttgtccttct gcggttgcat gctgccaggc attgagtaat tgttgttcgt cttcggcaca
     4261 tacaatgtgc tgagcattat gatcaatgcc aaatgtgttc cagggtttta aggagtggtt
     4321 catagctgct ttcctgatgc aaaaacgagg ctagtttacc gtatctgtgg ggggatggct
     4381 tgtagatatg acgacaggaa gagtttgtag aaacgcaaaa aggccatcct gacggatagc
     4441 ctt
//
LOCUS       DAJVQH010000205         4416 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJVQH010000205 DAJVQH010000000
VERSION     DAJVQH010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.207"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..203)
                     /locus_tag="OQM87_004000"
     CDS             complement(<1..203)
                     /locus_tag="OQM87_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HCS1509923.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGS"
     gene            complement(214..2511)
                     /gene="xdhA"
                     /locus_tag="OQM87_004001"
     CDS             complement(214..2511)
                     /gene="xdhA"
                     /locus_tag="OQM87_004001"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCS1509924.1"
                     /translation="MEAREATATGESCMRVDSIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATHTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASTGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAFESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            2926..3681
                     /locus_tag="OQM87_004002"
     CDS             2926..3681
                     /locus_tag="OQM87_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCS1509925.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            3760..3833
                     /locus_tag="OQM87_004003"
     tRNA            3760..3833
                     /locus_tag="OQM87_004003"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3792..3794,aa:Gly,seq:ccc)
     gene            3968..>4416
                     /locus_tag="OQM87_004004"
     CDS             3968..>4416
                     /locus_tag="OQM87_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1509926.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATEHLTVDQHRYINDVIGVLKACQDRLKG"
BASE COUNT         1115 a         1101 c         1130 g         1070 t
ORIGIN      
        1 gagccatctt ccgccagcgt aattccccgc agctccgcca gattatgaat atcaacaata
       61 tggcgataac ggtcattgtg atggtggagc tgaatcagta cgtcagtgcc accggcgagc
      121 agtttggcct gcgggttgtc agccagcagg ttgatggcat cggcaagggt tgctgcgcga
      181 tggtaagaag caaaatcaaa catgatgtta tcctcaaatc aatcctgcca gatggaactc
      241 ttcatataac cgttttggcg tcagcggcag tgtattgatt gcaacaccgg tagccatctt
      301 caccgcgtta cgaatagcag cggcaacagg aattatgggt ggctcaccca gtgacttatg
      361 tccgtatgcg gattgcggct cattgatttc gacgaacgcg ctttccagtt gtggcagatc
      421 cggcatggtc ggcattttgt aatccagcag attggggtta cggaccacgc cgcttttagc
      481 atcgatgatc atctcttcaa atagcgccca gccaatgccc attcccattc cgccgtgtac
      541 ctgaccttct gccagcagtg gattaagaat atgccctgaa tcatgaacgt tgaggatgcg
      601 gttgatggtg actttgcaca gcgcaatatc gaccgtcaga tcaacaaagg tacagccaaa
      661 cgccggtggg ttagtggtgg ttttgatgga gctttcagca gagagctgcc cgccgcgttc
      721 agggtggtag aaagcgtcca tcgccaaatc ttttaacgac attaacggct cttccggtcg
      781 ttcaaccagc acgatatggc cttttatcag ggtcaaattc atcgctgact gatgtagcat
      841 gactgcggcg tgagcgatga ttttctcttt taataatagt gccgcactgc gcagcgcagg
      901 cgcggcaaca tagctctggc gtgaggcaaa tgcgccggga tcgaacggcg taacgtcggt
      961 atcttgtgtt gaaataacgc gaacgtcgct gaccggaacc cccacggttt ctgccaccat
     1021 ttgcgagaag acggtgtcgg caccctgacc gatttccgtc gcgccgcttt gcacgttgat
     1081 ggttccatcc tgattcatca gaaggcgcgc gcctgctatt tctacgccga caggccaggt
     1141 gttagaggtg tagctaaaac aggcgacgcc aacgccgcgg cgcaaattgc cttgctggtt
     1201 ctggcattct gcacggcgtt tttcccattc aaagattttc cggccttttt caagacactc
     1261 cggcaaccct gcgctgcaaa tacgtttgcc cgtgagcgga ttagcatctc cttcgcgggc
     1321 ggcgttgcgt aaacgaattt caacaggatc aatacctaac gctgtcgcgg cgtcatcaag
     1381 catagactca aaggcaaata cgacttgtgg cgcgccataa ccacgcatcg caccagccga
     1441 ggggaggttg gtatagcagg tctttgaact gtaagcgtag gcacaacgag gataaaggta
     1501 agcgacttta ttcccccccg tagaagcgat ggagtgcccg tgagatgcat aagcgccggt
     1561 gttagacaga acatccagac tataaccttt caaggttccg tcgcggttca cgcccatttg
     1621 cccgtcaata gtaaaagcgt ggcgggtatg ggttgcgagg aaacactctt cacggctaag
     1681 ggaaactttc accggaatgc cgccaagctt gctggtcagg aatgccgcca ttggctcttc
     1741 cagtacatcc tgtttattac caaaaccgcc accgacaaac ggtttgatga ctcgtacgca
     1801 tgaccaggga atatccagcg cctgaccaac cacgcggcga acaatgtgcg ggatctgggt
     1861 gctggaaacg atggtaattc gcgagtcatc ctccatccac gccagcgatg ttacgctttc
     1921 catgtgacaa tgttgaataa cgggagtctg atagtgcccc tgtacctggt agtcggcggc
     1981 atcgattgtt tgttggacat tgcccgtcga catcgtgctt tgtttcagta aattgccgcc
     2041 gttatggatt ggtgcagcgt cttctgtcag cgccgcttct ggcgtggtga taacgggtaa
     2101 ttcttgccac tcaatgctga ccaattgcgc cgctttttct gccgtgagtt catcgcgggc
     2161 cacgacgatg gcaacggcgt caccatgatg acgaacatgg cgagttagca gtgcgcgatc
     2221 ggcggtatcg cgcttgtttt cgtcaagtgt ccaggcatgc ccagctgtag cgaatggaat
     2281 atcaggcaca tcttcccaag taaaaatcgc cagtacgccc ggtaaacttc tggcttgttc
     2341 atcattaata cttacggcat aaccatgtgc gatagggcta cgtacatatt tcgcgtaaca
     2401 catgcccgcc ataacataat cgtcagtata tcgtgcccgc ccggtgacct tagcaatgga
     2461 atcgacgcgc atgcatgatt cacccgtagc ggttgcttcc cgcgcttcca tagaaatccc
     2521 ctcgatttga acatttggtt cttatggcaa agatttagtc taagcaggca agatttacgc
     2581 cagaaacgac gataagcgtt aggggaggaa ggaagtgtga tgacgttaaa aaaacacaga
     2641 tatgatacga aacaggactc tgaataggga cagtgatacg tatcaatatg agtggcgata
     2701 aaatcaatgt gataaatcag gattattacg catcaggatg ggaaataggg taaaaagtca
     2761 ggaacttcga tacctctaca gcgaatattt tgctgatgat acttcccttt gccaaatgaa
     2821 attaacctct ttctctatta ccacgttttt ccagaagcaa gagattgggt atcaagaggc
     2881 tggctgctat gatatggcgt cttgtttttt aagcgaggaa agattttgag tgcgggacgc
     2941 ctgaataaaa aatctctggg tatcgtgatg tttttatcgg ttggactgct attggcgggc
     3001 tgttcgggta gcaaatcatc cgatacagga acgtattccg gctccgttta caccgtgaaa
     3061 cggggggata cgctatatcg tatttcgcgc accacgggaa ccagcgtaaa agagctggcg
     3121 cgactgaacg gcatttcccc cccttacacc attgaagttg gtcagaaact aaaactgggt
     3181 ggggcgaaaa gtagcagtag tacacgtaaa tcaaccgcca aatcaacgac caaaaccgca
     3241 tcggttacac cgtcatcagc ggtaccgaaa tcttcctggc cgccagtagg gcaacgttgt
     3301 tggttatggc caacgacagg gaaagttatc atgccgtatt cgacagcaga tggcggcaat
     3361 aaagggattg atatctcagc tccacggggt acacctattt acgccgcggg tgcaggaaag
     3421 gtggtgtatg tgggcaacca gctgcgtggc tacggtaatc tcatcatgat taaacacagt
     3481 gaagattaca ttacggctta cgcccataat gacacgatgc tggtaaataa tgggcaaagc
     3541 gtgaaggctg ggcaaaaaat cgccactatg gggagcacgg atgcggcatc tgttcgcctg
     3601 catttccaga ttcgttaccg tgcaacggca attgatccgc tacgttactt gccgccgcag
     3661 ggcagcaagc caaaatgctg atggcgaatt aatcagcagt cagcagcgcg gcacttgcta
     3721 aggagagcgt aaggtttata atgccttacg catctcgaag cgggcgtagt ttaatggtag
     3781 aacgagagct tcccaagctc tatacgaggg ttcgattccc ttcgcccgct ccatgacact
     3841 tcttaaagtt caataactta ctgaatcata agggatatgg cttaacgcct tccgatagtt
     3901 ttactcactg taaccaacac tgtgactaac tttgttgaag tagtgagcag acctaaggtc
     3961 tcagtacatg ctcaactcaa gaacctacct ttatcagcgt aatggtgtct tttacatccg
     4021 tcttaggatg aagaccactg gtcgccttac cgcttcgctt ccttctcaca accgttataa
     4081 actagcgtca gtatctttac ggaccaagga tagacgtacc gctatggctc actcccggca
     4141 catcaagtca gcacttaaag caatccatgc agacaaccct aacgcctctt atgaggagct
     4201 acgggagcac ctgaagacca ttgtcgagtg ggaactcagt gtcagccgcg atgacctgaa
     4261 cgaccctgag tcctatcagc tttacgttga ccagtacgac gatattaagt ccaaccttcg
     4321 ggaagctgta gcgactgagc acctgactgt agaccagcat cgctatatca atgacgttat
     4381 cggtgtgctt aaagcctgtc aggatagact caaagg
//
LOCUS       DAJVQH010000206         4407 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJVQH010000206 DAJVQH010000000
VERSION     DAJVQH010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.208"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..253
                     /locus_tag="OQM87_004005"
                     /pseudo
     CDS             1..253
                     /locus_tag="OQM87_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..1492
                     /locus_tag="OQM87_004006"
     CDS             746..1492
                     /locus_tag="OQM87_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1509927.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1507..1929
                     /locus_tag="OQM87_004007"
     CDS             1507..1929
                     /locus_tag="OQM87_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS1509928.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1987..2343
                     /locus_tag="OQM87_004008"
     CDS             1987..2343
                     /locus_tag="OQM87_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509929.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2436..2654
                     /locus_tag="OQM87_004009"
     CDS             2436..2654
                     /locus_tag="OQM87_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCS1509930.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2656..3018
                     /locus_tag="OQM87_004010"
                     /pseudo
     CDS             2656..3018
                     /locus_tag="OQM87_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3018..3254
                     /locus_tag="OQM87_004011"
                     /pseudo
     CDS             3018..3254
                     /locus_tag="OQM87_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3447..3683
                     /locus_tag="OQM87_004012"
                     /pseudo
     CDS             3447..3683
                     /locus_tag="OQM87_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207968.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3683..4048
                     /locus_tag="OQM87_004013"
     CDS             3683..4048
                     /locus_tag="OQM87_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCS1509931.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT         1175 a          934 c         1257 g         1041 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagatac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaattc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggtgaattt
     1441 taactgggag agctggcgtc cgaatgttag ccattcgagg gttgttaagt agtttcagga
     1501 ggatttatgg cgaaaccttt tactcccgaa cagcgggaag aactgaagac gcgaattgtg
     1561 gaactcgtgc atcaggacgg tcgggtcacg attcggcagt tgtcagatga aacaggtatc
     1621 agtcgtgcgt ctgtcggtcg cttatgcatg gaactggtcg caagtggtga tgtatataat
     1681 tctggctacg gggtatttcc ctctgaacag gctcataaag actggcaaaa cgcccgcaaa
     1741 aagctatcga gggtaaaggt gaagaaaccg gttgtggttg atcctgacct tatctggtca
     1801 ttaccagacg gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc
     1861 cggaagagcg aagctatgca gcgcgtgctg gcgttttatc aggggaaatt tcaggaggcg
     1921 gtactgtgag taaaattagc tatcaggctt caattaccgc tggtattcgc atcaaaggag
     1981 aggagcatgg aaataaaacc agaagatgag ttaagcaata tcgttttatt tccggtaaaa
     2041 gaggatgacc cacgtaatca ggttaatttt ctttatgaac catcggaaag accatattgc
     2101 catcacgcct ctgtccgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt
     2161 gcggttgtgg agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca
     2221 gatgatgtaa ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg
     2281 atacagattg agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa
     2341 taattaaatt tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca
     2401 aatcatcagg agaccgcccg aaagggcggt aatgaatggt tacattattt agaaaaaaat
     2461 atccgcgaaa gagtagaaca acagaattcc tgtttctcat tctgtttatc gtgttgatga
     2521 caccgatatc cccgctaatt tttgtctgga taatcggaaa aataattgag ccagttattg
     2581 aattgtataa cgacgtggta tgggcgtcat tcaacacact gcacaataaa attaatccgt
     2641 ataaggaaag ctgatatggc actgacgaaa aaacaacgtg cagaactgcg catgaagttc
     2701 ggtggtcgct gcgcttattg cggctgcgaa cttggcgaaa agtggcatgc agaccatgta
     2761 aaaccggtca ttcgttttga tggaaatatg cttcaccagg aacgtgacga tatatccaac
     2821 atggtaccag aatgccaccc atgcaatctg cacaagcatt gcagtagcct agaagattat
     2881 cgacgaatta tcagtgatgg tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg
     2941 gttcgtatgg gattggttga aatgaaatct gacccggttg tgttctggtt tgaaaaatat
     3001 caagaagggg ctacggcatg gccactatta ccaaagagcg actgcaatgg ctggctaaca
     3061 tttctggccg cgatgatatt gacgatatag acggcggtga aattcgtgaa ctggcgctta
     3121 tcgctctggc atcactggaa gcagagcctg tagcggaatg cattgttgaa gatgggggca
     3181 tgtgtattga cgggttcggt gagtatgtgg gtcactcgct gcctgatgga atgcatgagc
     3241 tttatgctgc cccgcgaatg cgacaacctg atggatggaa agccgtagct gtagcgtgga
     3301 aggtgacgtt tactcaagtt gaccaggaat ctaatacgtt cactgctata tattttgaca
     3361 aagcggaagt tgagcgttgg gtacgactgc ataaagcatg tgattttcgg gcagatataa
     3421 caccgcttta cgcagcgccg gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat
     3481 tccatcacgc actgtcagat tcatcgcttg gctctggtga aatcgaagaa attaaaaccg
     3541 gtttgcgtgc tgcctttgcc aacgtcacta tccaaccaga gccgatagtg ccggatgaaa
     3601 tcgggccaaa cgatagtaat acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg
     3661 ccatgcttaa gggagataaa taatgattaa tcgaaccaaa ctggagcaca ttctcgaata
     3721 tgccaggcag cagaaatgta ttgggcaact ttgtaaaatt ccaccaggag atatggttga
     3781 aatcgtggaa atggccatgc gtaaggctgg caactctccg gtaactctgg ctggctggat
     3841 aagctgtagt gatgcagttc ctgctgaata ttgcgatgtg attcttctcg atgatctcgg
     3901 gaatgtattc cccggttcct gggataaggt tttttgcccc actcgtggcg ggaataagat
     3961 ggcttttgtg gacaaagacg gcgttgaagt agagagctca actcactgga tgccgctacc
     4021 ggaaccaccg caggaggtga atcaatgacc tggcctgagg cattcaccac ggtaggaatt
     4081 gcgatggcgg tggcgctggt ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc
     4141 ccggattgat ggtccggggt ttttgaagga aacaaaacag aaacagcaat tgccgttacc
     4201 tgttgttacc atggcaagta aacgtatctc aggcgagcgc attgcgccgt tctgacgcag
     4261 ataaattagc ctggatagaa ggtgctggca ataaaaaata gcgttttctt atcggtatcg
     4321 gtaagattgc tgcgggtgct tgaggctgtc tgcctcgggc atgccactgt aaggcagaca
     4381 gagaaaagcc ccagttaaca ttacgcg
//
LOCUS       DAJVQH010000207         4378 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJVQH010000207 DAJVQH010000000
VERSION     DAJVQH010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.209"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(64..822)
                     /locus_tag="OQM87_004014"
     CDS             complement(64..822)
                     /locus_tag="OQM87_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCS1509932.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OQM87_004015"
     CDS             complement(830..1933)
                     /locus_tag="OQM87_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCS1509933.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3113)
                     /locus_tag="OQM87_004016"
                     /pseudo
     CDS             complement(1943..3113)
                     /locus_tag="OQM87_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3181..4206)
                     /locus_tag="OQM87_004017"
     CDS             complement(3181..4206)
                     /locus_tag="OQM87_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1509934.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1214 a         1147 c         1023 g          994 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 gaattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagga
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcttgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg caatattacg gaagatctca atataaatcg tggaaagctt tcgtagcagc
     2761 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     2821 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     2881 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     2941 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt ttgaaataac
     3001 caaccaacaa tgccgacaac agcaacaacg gcaaggatct gaaataacca ggcacgaacc
     3061 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3121 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3181 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3241 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3301 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3361 gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3421 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3481 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3541 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata
     3601 cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3661 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     3721 ggcgacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     3781 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     3841 gacgccggta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     3901 ggagagcaaa tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg
     3961 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4021 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc
     4081 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4141 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4201 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4261 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc
     4321 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtg
//
LOCUS       DAJVQH010000208         4367 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJVQH010000208 DAJVQH010000000
VERSION     DAJVQH010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.210"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            611..2260
                     /gene="pgi"
                     /locus_tag="OQM87_004018"
     CDS             611..2260
                     /gene="pgi"
                     /locus_tag="OQM87_004018"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCS1509935.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2722..2964
                     /locus_tag="OQM87_004019"
     CDS             2722..2964
                     /locus_tag="OQM87_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509936.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            3078..3716
                     /locus_tag="OQM87_004020"
     CDS             3078..3716
                     /locus_tag="OQM87_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS1509937.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            3713..>4367
                     /locus_tag="OQM87_004021"
     CDS             3713..>4367
                     /locus_tag="OQM87_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1509938.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     "
BASE COUNT         1101 a         1131 c         1123 g         1012 t
ORIGIN      
        1 gaaagcacaa tatcagcgct gcggttcatg gcgtcaaaat cagctacgct ggtaccgcca
       61 aatttggaga caacaatttc agacataact acctcatgtc aggggatcca ttttcagcct
      121 tggcacaagg gaagagcgga agacgggtgg gcgcagagcg atacttcgct actattttca
      181 cccagaagtg ctccaccact tgcgaaacgc ccgactgcga acgcttctgg tgacaaccca
      241 ggggattcag cccctgtagc cgatgatgaa cgtggccagc cgttcaatca cctcggcgat
      301 gcaccccctc aggtgttatc acaggactgg ctcctccaac accgttactt gggcaacgcg
      361 cctcttctgg cctgcgctag cgcaggtagt acatttataa ataaagggtg agcggggcgg
      421 ttgtcaacga cggggtcatg cggatttttc atccactcct ggcggtcagt agttcagcta
      481 ataaatgctt cactgcgcta agggtttaca ctcaacatta cactaacggc actaaaacca
      541 tcacattttt ctgtgactgg cgctacaatc ttccaaagtc acaattctca aaatcagaag
      601 agtattgcta atgaaaaaca tcaatccaac gcagaccgct gcctggcagg cactacagaa
      661 acacttcgat gaaatgaaag acgttacgat cgccgatctt tttgctaaag acggcgatcg
      721 tttttctaag ttctccgcaa ccttcgacga tcagatgctg gtggattact ccaaaaaccg
      781 catcactgaa gagacgctgg cgaaattaca ggatctggcg aaagagtgcg atctggcggg
      841 cgcgattaag tcgatgttct ctggcgagaa gatcaaccgc actgaaaacc gcgccgtgct
      901 gcacgtagcg ctgcgtaatc gtagcaatac cccgattctg gttgatggca aagatgttat
      961 gccagaagtc aacgcggtgc tggagaagat gaaaaccttc tcggaagcga ttatctccgg
     1021 tgagtggaag ggttataccg gcaaagcaat cactgacgta gtgaacatcg ggatcggcgg
     1081 ttctgacctc ggcccgtaca tggtgaccga agcactgcgt ccgtacaaaa accacctgaa
     1141 catgcacttt gtttctaacg tcgatgggac tcacatcgcg gaagtgctga aaaaagtaaa
     1201 cccggaaacc acgctgttct tggtagcatc taaaaccttc accactcagg aaaccatgac
     1261 caacgcccat agcgcgcgtg actggttcct gaaagcggca ggtgatgaaa aacacgttgc
     1321 gaaacatttt gcggcgctct caaccaatgc caaagccgtt ggcgagtttg gtattgatac
     1381 tgccaacatg ttcgagttct gggactgggt cggcggccgt tactctttgt ggtcagcgat
     1441 tggcctgtcg attgttctct ccatcggctt tgataacttc gttgaactgc tttctggcgc
     1501 acacgcaatg gacaagcatt tctccaccac gcctgccgag aaaaacctgc ctgtcctgct
     1561 ggcgctgatt ggcatctggt acaacaattt ctttggtgcg gaaactgaag cgattctgcc
     1621 gtatgaccag tatatgcacc gtttcgcggc gtacttccag cagggcaata tggagtccaa
     1681 cggtaagtat gttgaccgta acggtaacgt tgtggattac cagactggcc cgattatctg
     1741 gggtgaacca ggcactaacg gtcagcacgc gttctaccag ctgatccacc agggaaccaa
     1801 aatggtaccg tgcgatttca tcgccccggc tatcacccat aacccgctct ctgatcacca
     1861 ccagaaactg ctgtctaact tcttcgccca gaccgaagcg ctggcgtttg gtaaatcccg
     1921 cgaagtggtt gagcaggaat atcgcgatca gggtaaagat ccggcaacgc ttgactacgt
     1981 ggtgccgttc aaagtgttcg aaggtaaccg cccgaccaac tccatcctgc tgcgcgaaat
     2041 cactccgttc agcctgggtg cgttgattgc gctgtatgag cacaaaatct ttactcaggg
     2101 cgtgatcctg aacatcttca ccttcgacca gtggggcgtg gagctgggta aacaactggc
     2161 aaaccgtatt ctgccagagc tgaaagatga taaagaaatc agcagccacg atagctcgac
     2221 caatggtctg attaaccgct ataaagcgtg gcgcggttaa tcatcatgcg tgcctgatgc
     2281 cgatatgtag gccggataag gcgtttacgc cgcatccggc aaccggagcc tgatgccaat
     2341 atcgacatta atgcatagta tgaggagtta aataacctta aaaacaatgc cgacaataag
     2401 tcggcattgt tttatcagat aaatcccctt gtctgtgatt taacggaaat cataccgtga
     2461 ggttaatcct aaaatagatt tttaatcgtt gtttatttcg gagaatacgc agattaatta
     2521 cttttgtttt tattttaagt ttatgatttt tattgttatt taaatataag ttgaaactta
     2581 tatttgatat tcattccaat tatcctaaaa ctccatcgct aattccccgc gccgtaattc
     2641 gcatgcttta gttgtgtata ctcgatcccg cccgaaatgt ttttgggtaa atctccattc
     2701 attcaatgaa gggaaattgt tatgaaaaaa gttctgtatg gcatttttgc catatctgcg
     2761 cttgcggcga cttctgcgtg ggctgcacct gtacaggtgg gcgaagcggc agagtcggca
     2821 gcaacgtcgg tttcggcggg gagttcctcc gcgaccagcg tcagcaccgt aagctcggcg
     2881 gtgggtgtcg cgctcgcggt aaccggtggc ggtgatggtt ctaataccgg gaccaccaca
     2941 accacgacca ccagtaccca gtaataaagt atgtatcccc aaaataattt gagtcattgc
     3001 atctgtggct tgaagtatga agggatttac cataaccaca ctccggtgtg gttattctgc
     3061 ccctctggag aagagttgtg aagcgacctg cactcattct tatctgcctg ctattacagg
     3121 cctgttcagc cacgactaaa gagctgggca attcactgtg ggacagtctg ttcggcacgc
     3181 caggcgtaca gctgacggac gatgatattc aaaatatgcc ctacgccagc cagtacatgc
     3241 agcttaatgg cgggccgcaa ttgtttgtgg tgctggcctt cgctgaagac ggacaacaaa
     3301 aatgggtcac tcaggatcaa gccactcttg tcacacaaca tggtcgtctg gtgaagactt
     3361 tgcttggcgg cgacaacctg attgaagtga ataaccttgc cgccgacccg ctgattaaac
     3421 ccgcacaaat tgttgatggt gcaacatgga cgcgcacgat gggctggacc gagtaccagc
     3481 aggtacgcta cgccaccgca cgctcagtct tcaaatggga tggcaccgat accgtcaagg
     3541 tcggcagcga tgaaaccctg gttcgcgtgc tggacgaaga agtctccacc gaccaggcgc
     3601 gctggcataa ccgctactgg atcgacagcg aagggcaaat tcgccagtcg gaacagtatc
     3661 tcggcgcgga ttatttcccg gtgaaaacca ctctcatcaa ggcggcaaaa caatgattaa
     3721 acaaactatt gtcgcgttgc ttttgagtgt gggagcgtca tcggtcttcg cggcaggaac
     3781 cgtcaaggtg ttcagcaatg gcagcagtga ggccaaaacg ctaacgggcg cagagcattt
     3841 aatcgatctg gtagggcaac ctcgactggc aaacagctgg tggcctggtg cggtgattag
     3901 cgaagagctg gcaacggcgg cagcattgcg tcagcagcag gcgttgctga cacggctggc
     3961 agagcagggc gcagattcca gcaccgacga tgccgccgcc attaacgcct tacgccagca
     4021 aatccaggca ttgaaagtga caggcaggca aaaaatcaat cttgatcccg atatcgtccg
     4081 cgttgccgaa cgcggtaacc cgccgttgca gggcaactac acgctgtggg tcggaccacc
     4141 gccgtccacg gtcacgttgt tcgggcttat cagccatcct ggcaatcagc cattcactcc
     4201 aggtcgcgac gtggcgagct atctctctga ccagagcctg ctcagcggtg cggatcgcag
     4261 ctacgcgtgg gtcgtttacc cggacggacg cacgcaaaaa gcgccggtgg cttactggaa
     4321 caagcgtcac gtagagccga tgcccggcag cattatttat gttggcc
//
LOCUS       DAJVQH010000209         4273 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJVQH010000209 DAJVQH010000000
VERSION     DAJVQH010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.211"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            158..827
                     /locus_tag="OQM87_004022"
                     /pseudo
     CDS             158..827
                     /locus_tag="OQM87_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1070..1765)
                     /locus_tag="OQM87_004023"
     CDS             complement(1070..1765)
                     /locus_tag="OQM87_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCS1509939.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1758..3185)
                     /locus_tag="OQM87_004024"
     CDS             complement(1758..3185)
                     /locus_tag="OQM87_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCS1509940.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3196..3915)
                     /locus_tag="OQM87_004025"
     CDS             complement(3196..3915)
                     /locus_tag="OQM87_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCS1509941.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1146 a         1100 c          880 g         1147 t
ORIGIN      
        1 ccagatatgg ggaccgaatc gttatataaa acactgaggt aactctcatg cttcatatcc
       61 agtattcatg atgctggttt tttgctgata tttaacccga acattcctga tacagactat
      121 taatacaaca ttatcttcga cagattttag atatattatg agtgaacaaa tcaaacagga
      181 tatcgatctg attgagattt tatttcatct gaagaaaaag attcgtgtta tcctttttat
      241 tatggctatc tgtatggcaa tggtgctgtt gtttctgtat atcaataaag acaatacaaa
      301 agtgatttac agccttaaaa taaaccagac aacgccaggt atacttgtta gctgtgatag
      361 caataataat tttgcctgtc agaccacaat gactgaagat gttattcagc gaattactac
      421 attttttcag accagcccag atatcaagaa cagagaaata aggctggaat ggtcaggaga
      481 taagagagat ttaccaactg ctgaagagga aatatctcgc gtgcaggcgt ctattatcaa
      541 atggtatgcg tcagaatacc ataatggcag gcaagttctc gatgagatac aaacgccttc
      601 agcaattaac agtgagcttt atacaaaaat gatatacctg accaggaact ggtcattgta
      661 tccgaacggt gatggctgtg taactataag ctcaccagaa ataaaaaata aataccctgc
      721 tgccatttgc ctagctctgg gatttttttc taagcattgt aatttctgta atgttttgcc
      781 ttgtcaaaaa aatggtagat gaataccaac aaaactctgg gcagtaataa acagcattct
      841 gatagagagt gcctgtataa agctgaagtg acaatgaccg ggaagggaac ccgttttcag
      901 gaatggatgc ctgtcttttt cactgtaaat atcctggcat tactaatgcc tggatattta
      961 cagaagagca gcataaccat gacggaacgg ggttaagtca ggttgtcctt aacgctattt
     1021 tcaacctcgt atgccttctt caggtttatg ttcagatctc atatctctct cagcaatcct
     1081 caataatcag atacaccgct ttcaccggac catgaacccc gacgactttg ataagctcaa
     1141 tatccgccgt tgagctggga ccactgatga tgttaataca tgacggcatt cgttcaccgg
     1201 cctgcgcttt ctgatgcaat ttttctgcga gttgcgctac acgcggcagg attgtgcttt
     1261 tacgcaggat aaaaagagaa gattccggga gcaggctcaa tgaacgcccg cgctcggcgg
     1321 cggaaaaaag aaccacgcct cccgattcgg ttaaaccata ttcagcatac acgacaccca
     1381 ctttagcctg ctctgcctgc gagatattct cggcaccttt cgccggatcc caaacaacgg
     1441 cattgcattc ctgctgcaaa cgttcactaa tccccaattc ctccagcctc gtgtcaccgc
     1501 taatcacgac cgactgatct cccagctctt tacacagacg tattgcagct tctgccgcct
     1561 tcgcctcgct ggtcagctca cagcgcgtca acataacatc gctggcaaac tgaataaacg
     1621 cgtcacagcg ctgctgttgg ttaagttggg taagccgctc gttagcatag ttgttaagcg
     1681 gcgcatcttc tgcttgcggt tcaagtcgca gcgggcgacc cagtgcctga gcaacgttat
     1741 tcaaaaattc gctccgatta tccattcttt ttctcctgcg cctgatgttt cttaaaccaa
     1801 ctacggaaac tctctccgtc agcttcagga agatcgcgtg cttccatcca gtcgctaatc
     1861 gcgccaaatt tgagtggtgt tttaccgcca ttgataaacc agcttgccgc atgagcgccg
     1921 gccatcatcc cgactttcca caatcctgga tgactattgg cataagcgaa catttttatc
     1981 gcccgttgct ctgcttttgc ggtgatccct ttttcagcca tcacccgacg atgacgcaaa
     2041 atcagttttg acagcggaat acgcaccgga cacacgctgt cacaagcagt gcataaagag
     2101 caggcgtagg gtaaatcttt aaaatcttta tagccgccaa gtagcggaga aatcaccgca
     2161 ccaattggcc ctggataaat agagccatat ccatgaccgc caatatggcg atatgccgga
     2221 caagtattca tacaagcccc gcagcgaata cagcgcagca catcccgaaa ttcagaggcc
     2281 agcacctcag aacgcccgtt atcgacaata accagatgaa actcttcagg accatcaacg
     2341 tgccccgctt cgcgcggccc tgtcagccag gtgttgtatc ccgtcaaacg tgcaccaacg
     2401 gcactgcgcg ccagcatggt gatcaataca tctacctcgg caaacgtggg ggcaatacgc
     2461 tccattccca tcactgcaat atgcgtttta ggcagcgtgg tacacattcg cgcattacct
     2521 tcattggtca ccaggcatac cgaaccggtc tctgccaccg cgaaattaca gccggtaata
     2581 cctatttcag cactgaggaa atcttcacgg attttttgcc ggatgaataa ggtcatcgct
     2641 tcaggcgttt ccggcccctc atagcccaga cgttcgtgta gcactcgacg gatctgatgg
     2701 cgatctttat gaattgccgg gaccacaaca tgagatggcg gatcttgatc cagctggaga
     2761 atatattcac ccagatcggt ttcaatcacc tgaatgccag catcctgcaa cacatgattg
     2821 acaccaatct cttcggtcac catcgattta gatttcacca ccttccgggc atttttgcgt
     2881 tgggcaacct gtaaaatgta gcgggtagcg tcttctttgg tttttgcaaa atagacgtga
     2941 ccgccgtttt gcgtcacttt ttctgagagc tggtacagat aagcgtcgag attactcaaa
     3001 acatgatcac gtatctgggc ggcccgatcg cgccactcct cccagtgccc caattcatcg
     3061 accatttttt gccgatttgc cccaatacgc tgctgcgcgt ttgccaccgc tttgcgcatg
     3121 atcggatctt caatttgctg acggatgcgt gtcttaaaat ctgtattact ggttttgatc
     3181 gacatcttta tatcctcagc ggctcatcaa cacttcagca atatgcatca ctttgacttt
     3241 ctgcccttcc cgttgtaatc gcccactgat gttcagcagg caactcacgt cagcaccaat
     3301 taaatactca gggcggactt ccatcaggtg cacaaccttt tctttcacca tctcgccgga
     3361 tatttcggcc attttgaccg agaacgtgcc gccaaatccg cagcaggtat cctgttcagc
     3421 aaaggtcaac agctccagtc cacgcacatt tttcagcagc gtaagtggct cgtccttcac
     3481 tcccagctta cgggccaggc tacaagatgg gtgatacaca gctctccctt gcaaactggc
     3541 acctacatcg actaccccta atttattaac aataaaagag gtgagatcct gcatacgcgc
     3601 ggcaaccttt tcggcacgtg atgcccattc aggttcatcc gccagatacg tcgggtaact
     3661 ttttacggca taggtgcaag agccagccgg tgaaataatg ggatcgtcgt tatcctccag
     3721 tgcggcgatc agatttttca tccctggaat cgcttctttg atataaccgc tattgatcgc
     3781 aggctgaccg cagcatccct gtttctccgg gaaatttacg cgacagccga gtttttccag
     3841 tagcagcacg gagtctcgtg ccattcttga tttcagggcg tcaccaatac aggtgacaaa
     3901 gaaattgaca ttcacaacta atactccatt acttcatgcc catttatgcc cgatgtttcc
     3961 cccccaccgg gggatcaatc atcaataaat atggcgttct ttttaaattg gctagcaaca
     4021 taccacccct catttgtatg acaaatgata atctttcatc caaacttcaa atgcaataac
     4081 taattttatt tctttcggca tcgctcacat tttatgggga aatcgacgat catttttggc
     4141 ataagaaatc catttaaaaa tggctacatc ccttatacct attcagagct acttattcag
     4201 gggttattga gatatacact gatggtgtac aaaaaaatca ccgacaataa tcacgtatca
     4261 agtaatagtc gga
//
LOCUS       DAJVQH010000210         4212 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJVQH010000210 DAJVQH010000000
VERSION     DAJVQH010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.212"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..103)
                     /locus_tag="OQM87_004026"
     CDS             complement(<1..103)
                     /locus_tag="OQM87_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCS1509942.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPS"
     gene            complement(253..414)
                     /locus_tag="OQM87_004027"
     CDS             complement(253..414)
                     /locus_tag="OQM87_004027"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509943.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(411..599)
                     /locus_tag="OQM87_004028"
     CDS             complement(411..599)
                     /locus_tag="OQM87_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS1509944.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            859..1194
                     /gene="iraM"
                     /locus_tag="OQM87_004029"
     CDS             859..1194
                     /gene="iraM"
                     /locus_tag="OQM87_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1509945.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1475..1606)
                     /locus_tag="OQM87_004030"
     CDS             complement(1475..1606)
                     /locus_tag="OQM87_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCS1509946.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2106..2182)
                     /locus_tag="OQM87_004031"
     tRNA            complement(2106..2182)
                     /locus_tag="OQM87_004031"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2146..2148),aa:Arg,seq:tct)
     gene            complement(2284..2359)
                     /locus_tag="OQM87_004032"
     tRNA            complement(2284..2359)
                     /locus_tag="OQM87_004032"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2324..2326),aa:Ile,seq:cat)
     gene            complement(2503..3324)
                     /locus_tag="OQM87_004033"
     CDS             complement(2503..3324)
                     /locus_tag="OQM87_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCS1509947.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3339..3695)
                     /locus_tag="OQM87_004034"
     CDS             complement(3339..3695)
                     /locus_tag="OQM87_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1509948.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3708..>4212)
                     /locus_tag="OQM87_004035"
     CDS             complement(3708..>4212)
                     /locus_tag="OQM87_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1509949.1"
                     /translation="VLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLP
                     HAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRSWVNEKYKRWVKTQPCACCGKPAD
                     DPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFLDHA
                     FATGVLG"
BASE COUNT         1161 a         1133 c          830 g         1088 t
ORIGIN      
        1 gagacgggga aaacctgtcc agcaactgca ggaaccagta gcccgttccc accgctgacg
       61 tggtgtatga cacacctgtt gtgatttttt ccatctggtc cataccccgt ctcccgttat
      121 ccggaagctg acaacaataa aaaagccacc agttaactac tgatggctct gataactcat
      181 gcaagcgtct cagacgaccc actgacacta ccggtgagtt taacgatacc ttccatttga
      241 ctggctcact ttttatgatg atgccggtgc atttatctcc agcaccagac tttctatctc
      301 aacgccatac gttgcatttt tggtaatatc cgtcagcgtc agtgcattta gtcccactgc
      361 cagactgtct tttatggcct ggaatgccgg gccagtacga tgacgtagta tcactccggc
      421 tcagttgcac cactgaccac cacatcacct tctgctgcaa tcgcctgcat cagggtataa
      481 ggggttatgg ccaccggact accaaacggc tgccagccct ctttcagttt atgtgtcagc
      541 ttttccgcaa gatctgacgg cggcgccgcc ctgacaacat catagtgttt aaatgccatg
      601 gttctttcca ctatctgaaa aataattctt taaaatacct gacatgtaat acagaaaaaa
      661 cacaaaacca taccttaaat aaaaacctga ttatcaagca gatatgcatg gataaactac
      721 aagacgagat ataaaccacc ctgcatttaa ataaacaata aacaacatca gaaaaataat
      781 tctgctctat ggtttaattc aaaaatatca tttatacttt tcagaacatc accagcaagg
      841 cataaacaag gaaactaaat gaagtggatt gtgattgata cagttatcca gccatcatgc
      901 ggaatatctt tttcagtcat atggagtaaa ataaaattaa taatctggta tcaatcggat
      961 gctttcttac ctcctgaaag tatatttaca ctgactcaca caggcatcat gctcaataac
     1021 aaagtgctac ctgtaaccat ttacaacgta gtaccattca ataaaacatt ctggaattta
     1081 atcaaaaaca gccaggaatg ccctacaaat acagataacg tattgaatga atgctttaat
     1141 aaccgttgca ctctgcaaat atgtccttat ggactaaaac aacaaagtcc ataaggagtt
     1201 tactcacatc tgacaaaatc aatataaaca gcccctccgg agaggggctg gagagtggcg
     1261 ctatgtgcca ttgcatggtg ccgggtgcct cccggtgaat tcagtaccag cacctgaatc
     1321 cgcgattatc ccatatacct actcgctgat tgcccctccg cacaggggga ttcaccatgc
     1381 cagtttcttt taacaaactc cccgcaaacc agacaacagt caaccgcctg aattgtgaag
     1441 tatttaaaaa tttctcccgc taactgatac ccggctaaca gtctggcgtt ttctttttca
     1501 gcaacgggaa agcaacaacc accacacccg ccaccagcac accgtcagcc ggcactgaca
     1561 ttatccggct gctgcaatgc cattcacaaa aacagtaagc aatcactttt taccgtaaca
     1621 ggtgataatc cagatatgta tctaccccag atgagtaatc cgaagttcat ccataccaca
     1681 ggtcctggct attctgttgt actcctgaac aagagcaaat aattctgaat tagcaaccat
     1741 gaactcatcg caaaccctct gtatagcatc actattcaga ataataacgt ctcttcccga
     1801 aagacgatca ggagtacaga acaaaactgt caaacggctg aaggcctttg ctcgtgctgc
     1861 attgactata tcaatacgct gcctaaggat gaaacacccc gacgcctcat caatattcac
     1921 tctacccaca ccatatgaat gataaatact taatgctgaa aaaaccatta gaccgtataa
     1981 caaatactca atcaatactt aacagaactt ttatttttga caaatatata atattttcaa
     2041 caatatcctg agccaggtat atttcagtat aaggctctgc cggaaggaat ctggaagaat
     2101 gaatatggcg cgctgtactg gattcgaacc agtgaccgat tgcttagaag gcaattgctc
     2161 tgtccggctg agctaacaac gctgaatacc gataatggac cgccatcggg gacccgcccc
     2221 ccgcaccaac aaccctgtta tcgtgtcgtc tgctcttcct gataagctaa tggcggtttg
     2281 tgatggtggc ccttgctgga tttgaaccag cgacctggcg attatgagtc gctcgctctc
     2341 accactgagc taaagggccg ggagcagaat aataacggtc cgtaattaat ttcgcaataa
     2401 aaaacccgct cagcggcggg ttgtagaaac tcttctaacg tcaggcataa aacgcccatc
     2461 attatgacga atttaccaca gattccggaa aaatcaacct tgttacctag ttaccttttt
     2521 taactgccgc tcagcccatg cttcttcaat atcaaaccgg gtcaccagcg catcataaaa
     2581 tttcttaact gttttttccc atgacgcgcg tgttatctgg tttgtcacct cgcatatagc
     2641 attaaatgcc tccgttgatg gtagtctttc atagccacga ccaccacaac gctggcagtc
     2701 tctgataaca ggcataccac gttttaccga ctcttcacga tgaatggcga caccacgccc
     2761 acggcaatcc ttacaggcgg tggaaacctc accctttccg ccacactccg gacaggcaac
     2821 ttttaccacc tccctgactt ttttccattc ttcccagtaa gacggataca cacctttcgt
     2881 acactttgcc cataccggcg gcttaccatc cggatactgg atcttgtttg taaaaactat
     2941 gctttcaata aatttttccc catagcaaca agggcactgc tttttactcg ctgcgctgcg
     3001 ggcataatcc tcaaaagcgt acgaagccat aatgcgcatc actaccggtt ttatttctgc
     3061 cggaagtttt ctcaacgccg ccacacgatc gcaccgactg agtgcataat ctgccagtaa
     3121 ttctgttgcc cgcgccctgt cattcatact gatgcccatt ttccccagga acgcagaaaa
     3181 acccatctca gcccgattct gtgtcatgcc ctgcgcggcc atcacatcag tgatactcag
     3241 cgcatctttt gacgttgagg ccgatgcatc ggtcaggcca ggggattttg gggagtagta
     3301 tttcggtaaa tcttccagtt tcattttttg acctgctctt aatgcattat ggggtaaatc
     3361 ttcaccccca gacgtccacc agatactggc tgaccacgaa cgatattgat ttcatcaaac
     3421 tgctcatcgt ccattaacac tcccgcatgc gtcagcgcat ccagcggtgc tttcaggata
     3481 ttgtccaggt cgcgacgacg cttatccggt ggctctgcaa tcacctttat cgccagcctt
     3541 ccggacaggc ttaatttcag ccgctgctgg cgaacaataa gcgccacagc ccggcgataa
     3601 cgctttccct cctccgagat aaaatatgtg ctgccacggc gtcgccagta agtgttcacc
     3661 gtcggcgggt aaggtaaaac caaatctatg agcatcagtc acctctttta cccaagcacg
     3721 ccagttgcaa aggcgtgatc aagaaaacga aaaattaaat caacctgaga accatgcttt
     3781 tcttcgaacg ccagaggatc cgcatgaagc tcgttgtgat gctcccgaca cagcggtagc
     3841 gtgaaaatat cgtgagattt tgtccccatt ccgccctgac catgaccaat caggtgatgg
     3901 ggatcgtcgg ctggcttacc acaacacgca cacggctgtg tcttcaccca gcgtttgtat
     3961 ttctcgttaa cccagctgcg acgtttaggt cgtttcatga aagattccgg agactcagga
     4021 tcaacggcaa tgctgaccac cgtcttttcc tgttgtgggt tctgttgctg gtgggcgtga
     4081 ggcagcggcg caagattttt tgtgcgctgt ttcagtatgc tggtggcggt ctgctctccc
     4141 ggtacgatgt cgctttcacg gtacattgag cggatttttt ccgcacgcaa ccccagcgaa
     4201 cgacgtaata cc
//
LOCUS       DAJVQH010000211         4190 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJVQH010000211 DAJVQH010000000
VERSION     DAJVQH010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.213"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..275
                     /locus_tag="OQM87_004036"
     CDS             <1..275
                     /locus_tag="OQM87_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCS1509950.1"
                     /translation="FFVQVWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVEL
                     GKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF"
     gene            334..537
                     /locus_tag="OQM87_004037"
     CDS             334..537
                     /locus_tag="OQM87_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCS1509951.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            537..1562
                     /locus_tag="OQM87_004038"
     CDS             537..1562
                     /locus_tag="OQM87_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1509952.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(1615..1704)
                     /locus_tag="OQM87_004039"
     tRNA            complement(1615..1704)
                     /locus_tag="OQM87_004039"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1668..1670),aa:Ser,seq:cga)
     gene            1798..2595
                     /gene="mtfA"
                     /locus_tag="OQM87_004040"
     CDS             1798..2595
                     /gene="mtfA"
                     /locus_tag="OQM87_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCS1509953.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2696..2771
                     /locus_tag="OQM87_004041"
     tRNA            2696..2771
                     /locus_tag="OQM87_004041"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2729..2731,aa:Asn,seq:gtt)
     gene            2933..>4190
                     /locus_tag="OQM87_004042"
     CDS             2933..>4190
                     /locus_tag="OQM87_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1509954.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFI"
BASE COUNT         1163 a          964 c         1010 g         1053 t
ORIGIN      
        1 aattttttgt tcaggtctgg ggaaatggag ccaacttcga caacacgatt ttgcgccgtt
       61 catacgaacg gcaggggatc ccctgcccgt ggcgttacta caacgatcgc gatgtacgca
      121 caatcgttga gctggggaaa gccatagact tcgatgccag aacggctatt ccattcgaag
      181 gtgagcgcca taatgcactt gatgacgctc gttaccaggc aaaatacgtt tcagctatct
      241 ggcaaaaact gatcccgagt caggctgatt tttaatgttc aaccctaatt gccgctaacc
      301 gtatatagtt agcggcggtt atgagatata gctatgagca gcttattttt aaccgaagat
      361 gaattgctaa tattaacggg ctgcaaatat gcaagccacc agcgaaaatg gttaatggaa
      421 aacgggcttc cgttctatac caatcgtagt ggcaaaccga ttgtcagccg ggatctattt
      481 acctgcaata aaactttacc accacgcgag gtagagccga attttggtgc gatctgatgg
      541 gaagacgaag gaaaaatcct gaacacgaaa aattacctcc aaatgtatac ccaaataaat
      601 atagttatgt atggaaacca acatccagag aatctgtaac actaaccgcc atcaaggatg
      661 gtttagctgc tttatggaaa aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga
      721 cattcggtcg cttgtgggaa aaattcctcg ccagcgccta ttacagtgac ctcagtccaa
      781 gaacacaaaa agattatctg caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc
      841 cggcagattc cataaaacca gaacacatcc gtcgatacat ggacaagaga ggggaacaga
      901 gtaaaacgca agccaaccat gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg
      961 agcgagggta cgtgaaggct aacccatgtg caggtgtaag taaattcaag gccaaaaacc
     1021 gcgaacgata tgtaaccgac aaagaatacc aggcagtatt aagcgttgca cctcttcctg
     1081 tttttatcgc aatggaaatt gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc
     1141 tgaaatggga gcagattgga aacgacggga tctttatcca gcaagggaaa acaggaaaaa
     1201 aacagataaa agcatggagt ccacgattac aggcggcgat cgaaaaagca aaacagttac
     1261 caacatccgc ctatgtaatc agcaatcaat acggcaaccg atatatgtac aaaggcttta
     1321 acgaaatgtg ggtagaagca agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact
     1381 tcacctttca tgatctgaag gcgaaaggaa tttcagacta tgaaggaagc agtcgggata
     1441 agcaactttt ctctggtcac aaaaccgagg ggcaagtgct aatctatgac aggaaggtta
     1501 aagtttcacc gacacttgat gtcccgttac ctgaaaatat tccaagaaaa tattccaagt
     1561 aattccaagt gtgatttttg tcactgactt aatgatgtat aagtgattga attttggcgg
     1621 agagaggggg atttgaaccc ccggtggagt tgcccccact ccggttttcg agaccggtcc
     1681 gttcagccgc tccggcatct ctccgctcag atggttgcca tgatgccagg aaatttggca
     1741 ttttaacagt ccctgtccgt gcaattttgt tcaagtgacg agtttgcgag caaaacgatg
     1801 attaagtggc cctggaaagt acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa
     1861 gcactatcga tccccctttt aacgtgtctg acagaacagg aacaaagcaa attggtcgct
     1921 cttgccgaac gttttttaca gcaaaaacgg cttgttcctt tacagggctt tgagctgaat
     1981 tcattaagaa gctgccggat agcacttcta ttttgcctgc ccgttctgga gttaggactg
     2041 gaatggctgg atggttttca tgaagtctta atttatcctg cgccatttgt ggtcgatgat
     2101 gaatgggaag acgatatcgg tctggtgcat aaccaacgta ttgttcagtc aggtcagagc
     2161 tggcagcaag ggcctatcgt tttgaactgg ttggatatac aagattcttt tgatgcttct
     2221 ggttttaacc tgattattca tgaagtcgct cataagctgg acacccgtaa cggcgatcgc
     2281 gccagcggag ttccctttat ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat
     2341 gctgcaatga acaacattca ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt
     2401 gatgcttatg ctgccagtga tcctgctgaa tgttttgccg tactttctga atatttcttt
     2461 agcgccccag aactttttgc tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt
     2521 tatcaacaag atcctttgca gagactgcat cacgctaatg atacagactc gttttcggcg
     2581 acgaatgttc attaattaac aactttgcag attaattaac caattgaaat ggcttatgaa
     2641 atttagtgtt gacagacaag gtaccgctaa gtaatatgcg ccccgttctc acgattcctc
     2701 tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt
     2761 cagaggagcc aattttctgt tttcatgcat ccttgcgaat ctttatctga tgttgattca
     2821 acaggttagt gtgaaaactc ttctcggtgc attttgattt taccctacgc atcgggaaaa
     2881 attggtggtc aaatctggga tcaggttagt tcgataatgg agtgaccccc atatatccct
     2941 taccgacgca aaaatccgca ccctcaagcc ttctgataaa ccctttaaag tctccgattc
     3001 tcacggtctg tatctgctgg tcaagccggg tggctcccgc cactggtatc tcaaataccg
     3061 tattagcggt aaagaatccc gcattgcgct gggtgcctat ccagccatct ccctgtctga
     3121 tgcgcgacag caacgtgaag gtatccgtaa aatgctggcg ctgaatatca acccggtaca
     3181 gcagcgggct gctgaacgtg gctcacgaac accggagaaa gtttttaaaa acgtggcgct
     3241 ggcgtggcat aaaagtaaca ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag
     3301 cctgaacaat cacatctttc cggtcatcgg gaacctacct gtatcagaac ttaaaccccg
     3361 tcatttcatt gacctgctga aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg
     3421 cacacggcag cacctgagta acataatgcg ccatgcggtc catctggagt taatcgatac
     3481 gaaccctgca gcaaaccttg gcggcgtgac cacacctcct gtcagacggc actatcctgc
     3541 cctgccgctg gagcggctgc ctgaactgct tgaacgtatt ggggcatatc atcagggccg
     3601 tgaactgacc cggcatgccg ttctgctgat gctgcatgtg ttcattcgct ccagtgaact
     3661 gcgtttcgcc cgctggtcag agattgattt cacaaaccga gtctggacga tacccgcgac
     3721 gcgagaaccc attattggcg tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca
     3781 tatcgtcccc ctctcagaac agtccatcgc cattctgaaa cagattaagg atatcaccgg
     3841 taataatgaa ctgatcttcc ccggcgacca taacccgtat aagccaatgt gtgaaaacac
     3901 ggtcaataag gcactgcggg tgatgggtta cgacacgaaa aaggatatct gcggtcacgg
     3961 cttccgggca atggcatgca gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt
     4021 agaacgccag atgagtcatc aggagcgcaa taccgtgcgc atggcttata ttcataaggc
     4081 agagcacctg gaagcccgca aagcgatgat gcagtggtgg tcggattatc tggaagcatg
     4141 ccgagaatct tatgcaccgc cttatacaat tggtaaaaat aagtttatcc
//
LOCUS       DAJVQH010000212         4171 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJVQH010000212 DAJVQH010000000
VERSION     DAJVQH010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.214"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            261..854
                     /gene="tetC"
                     /locus_tag="OQM87_004043"
     CDS             261..854
                     /gene="tetC"
                     /locus_tag="OQM87_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCS1509955.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            complement(967..2172)
                     /gene="tet(B)"
                     /locus_tag="OQM87_004044"
     CDS             complement(967..2172)
                     /gene="tet(B)"
                     /locus_tag="OQM87_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCS1509956.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            2254..2877
                     /gene="tetR(B)"
                     /locus_tag="OQM87_004045"
     CDS             2254..2877
                     /gene="tetR(B)"
                     /locus_tag="OQM87_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCS1509957.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(2855..3541)
                     /locus_tag="OQM87_004046"
     CDS             complement(2855..3541)
                     /locus_tag="OQM87_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1509958.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(3549..3935)
                     /locus_tag="OQM87_004047"
     CDS             complement(3549..3935)
                     /locus_tag="OQM87_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCS1509959.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            complement(3928..4143)
                     /locus_tag="OQM87_004048"
     CDS             complement(3928..4143)
                     /locus_tag="OQM87_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS1509960.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
BASE COUNT         1371 a          878 c          746 g         1176 t
ORIGIN      
        1 ttggtataac ccgctttatt cgccacatca tcaagcagta aagactgatc taaattatgt
       61 tcaatccata atagaacatc tttgataacc gttgtatgaa actgcttatc atcatatctt
      121 aattggatgt tattagcttt attttgatag cgagaatgct gttcaatata cataaaataa
      181 cctaaatgtt cttaagattg tcacgaccac atcatcatga taccataaac atactgacgg
      241 tatgttattt taaatctatc atggaaaata aaaatcatca acaagaaaat tttaagagta
      301 cctatcaatc actggttaac tcagcacgaa tattgtttgt tgaaaaaggc tatcaagctg
      361 tttcaataga tgagatctcg ggaaaagcgt tggtgaccaa aggtgccttt tatcatcact
      421 ttaaaaataa aaaacaatta ctcagtgcct gttataagca gcaattaatt atgattgatg
      481 cctacatcac aacaaaaact gatttaacaa atggttggtc tgccttagaa agtatatttg
      541 aacattatct tgattatatt attgataata ataaaaacct tatccctatc caagaagtga
      601 tgcctatcat tggttggaat gaacttgaaa aaattagcct tgaatacatt actggtaagg
      661 taaacgccat tgtcagcaaa ttgatccaag agaaccaact taaagcttat gatgatgatg
      721 tgcttaaaaa cttactcaat ggctggttta tgcatatcgc aatacatgcg aaaaacctaa
      781 aagagcttgc cgataaaaaa ggccaattta ttgctattta ccgcggcttt ttattgagct
      841 tgaaagataa ataaaataga taggttttat ttgaagctaa atcttcttta tcgtaaaaaa
      901 tgccctcttg ggttatcaag agggtcatta tatttcgcgg aataacatca tttggtgacg
      961 aaataactaa gcacttgtct cctgtttact cccctgagct tgaggggtta acatgaaggt
     1021 catcgatagc aggataataa tacagtaaaa cgctaaacca ataatccaaa tccagccatc
     1081 ccaaattggt agtgaatgat tataaataac agcaaacagt aatgggccaa taacaccggt
     1141 tgcattggta aggctcacca ataatccctg taaagcacct tgctgatgac tctttgtttg
     1201 gatagacatc actccctgta atgcaggtaa agcgatccca ccaccagcca ataaaattaa
     1261 aacagggaaa actaaccaac cttcagatat aaacgctaaa aaggcaaatg cactactatc
     1321 tgcaataaat ccgagcagta ctgccgtttt ttcgccccat ttagtggcta ttcttcctgc
     1381 cacaaaggct tggaatactg agtgtaaaag accaagaccc gctaatgaaa agccaaccat
     1441 catgctattc catccaaaac gattttcggt aaatagcacc cacaccgttg cgggaatttg
     1501 gcctatcaat tgcgctgaaa aataaataat caacaaaatg ggcatcgttt taaataaagt
     1561 gatgtatacc gaattcgatt gcgtctcaac ccctacttcg gtatctgtat tatcacgtgt
     1621 atttttggtt tcacggaacc aaaacataac cacaaggaaa gcgacaatat ttagcaacgc
     1681 agcgataaaa aagggactat gcggtgaaat ctctcctgca aaaccaccaa taataggccc
     1741 cgctattaaa ccaagcccaa aacttgcccc taaccaaccg aaccacttca cgcgttgaga
     1801 agctgaggtg gtatcggcaa tgaccgatgc cgcgacagcc ccagtagctc ctgtgatccc
     1861 tgaaagcaaa cggcctaaat acagcatcca aagcgcactt gaaaaagcca gcaataagta
     1921 atccagcgat gcgcctatta atgacaacaa cagcactggg cgccgaccaa atcggtcaga
     1981 catttttcca agccaaggag caaagataac ctgcattaac gcataaagtg caagcaatac
     2041 gccaaagtgg ttagcgatat cttccgaagc aataaattca cgtaataacg ttggcaagac
     2101 tggcatgata aggccaatcc ccatggcatc gagtaacgta attaccaatg cgatctttgt
     2161 cgaactattc atttcacttt tctctatcac tgatagggag tggtaaaata actctatcaa
     2221 tgatagagtg tcaacaaaaa ttaggaatta atgatgtcta gattagataa aagtaaagtg
     2281 attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa
     2341 ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg
     2401 gctttgctcg acgccttagc cattgagatg ttagataggc accatactca cttttgccct
     2461 ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct
     2521 ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag
     2581 tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat
     2641 gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa
     2701 gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta
     2761 ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc
     2821 cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtcttaaaag
     2881 cagcataacc tttttccgtg atggtaactt cacggtaacc aagatgtcga gttaaccacc
     2941 ctttagattc ataaagcgaa aataatgcgg ctccaacgta cccacctaaa tggaaacggc
     3001 gttcactcca atctaaacac gcacaacaga ttttacgtga atgtttggaa ggaacgtcaa
     3061 ttcccatttc atgaaaatat tgaataccac ttaatgtgat cattgaacca ttttcagtga
     3121 tccattgctg ttgacaaagg gaatcataga tcttaacggc aacttcgcca gctaaatgat
     3181 catagcaagt acgtgctttt cgtaaatgca ctggcgtgga aactttggca tgtacgccat
     3241 ggtttaagga gatccccatc atactttcca tcaattcagc aatatctttt cctgctagcc
     3301 gaaaataacg atgcttgcct tgagctacta ctgtgattag ctggcaatct aataatttag
     3361 ataaatgact gctcgccgtt gaagctgata tattcgccac agaacttagc tcagtggccg
     3421 tccaagctcg cccatccatc aaagcactga gtattttaac tcgtgaaatg tcagacatag
     3481 ccgcccctat cgcggctatt gaggactcaa aggtaacctc ttttcgtatt aaattagcca
     3541 tcgcaagttc actttattgc ccaagggagc gtaacagatg cagccatact atcattgtcc
     3601 gttattaata tcagttggtt agcatggtca ctgtattgca ctaaaatatt aatgttattc
     3661 tccgccaata ctcgtgctat ttcgccaagt tcccccggtt tttcctgttt taacttacga
     3721 attaatggtg tccggatcgc aagtactaac agtccagctt gctctagcgc tattttagct
     3781 tcctttcctt gttcaacaag aaaatgagca tggcattcat caccaaccgt aaatatccct
     3841 cccccttcca atccaatacc tttattacct aatgtttttc ctagtaatgc taattgtcct
     3901 atttgattat ctaaaacaac gtgaacatca aacattatcc ttgtcctttt taaatgaata
     3961 ttcgcgagta atatgagcaa tactaatttt gtaaaaatca aatattgact ctcgcccttc
     4021 ttgttgagct ttcgcatgta aaacatgatt tttccattgc agaactgcgt attcgttttc
     4081 ccaccaagat agcgataaca tttttccttc tgtagctaga ctttgaaaac gttcaattga
     4141 aataaaacca gctacatgac ttaatagtgg t
//
LOCUS       DAJVQH010000213         4156 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJVQH010000213 DAJVQH010000000
VERSION     DAJVQH010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.215"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(242..772)
                     /gene="cybB"
                     /locus_tag="OQM87_004049"
     CDS             complement(242..772)
                     /gene="cybB"
                     /locus_tag="OQM87_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCS1509961.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            961..1962
                     /gene="gap"
                     /locus_tag="OQM87_004050"
     CDS             961..1962
                     /gene="gap"
                     /locus_tag="OQM87_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1509962.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDNTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVMQLIRTLEKFAKL"
     gene            complement(2004..3443)
                     /gene="aldA"
                     /locus_tag="OQM87_004051"
     CDS             complement(2004..3443)
                     /gene="aldA"
                     /locus_tag="OQM87_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCS1509963.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3640..>4156)
                     /locus_tag="OQM87_004052"
     CDS             complement(3640..>4156)
                     /locus_tag="OQM87_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCS1509964.1"
                     /translation="RAEATIQVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITLLNQ
                     AVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFIN
                     QLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAV
                     LIEAMESRSLR"
BASE COUNT         1093 a         1039 c          958 g         1066 t
ORIGIN      
        1 acctccatgg tttatcgaat cttatgtgcg atttgaacgt tatacgctgg tttattttga
       61 taccgttgat gagatcaaag atcgggtgaa ttgattaaaa ataagccaga tggacgtatg
      121 ggcaaggatt atggttttat ttgtcataca aataaatatg ataggcgctt ccgatgtaga
      181 cccgtattct tcgcctgtac cacgggtcgg ttttagtaca ggcgttttct ttaaatatcc
      241 ttcaggaacg tttacgcggc atcatgcgta gaagtgtgtt gtccttccag aaatagtggt
      301 gtgccagtgc agccgcagcg tgcaacccga tgacaaaata tcccagattc gccagcgtct
      361 catgccacga ctttaagcta tcaacccgtt cgaaattggc ctctgaagcg taaggcatcg
      421 tcaaaccaaa cgcaaaccac gggttgcccc ggttatacat catcaccaaa ccaatcactg
      481 gcagcgcaat aaacaggaga taaatcacca aatgtcccaa atgcgccagt cccgtcatca
      541 tcggtttcgg cttaggtata atcggcgggg ttgggtattt cagtcttaac aaaagacgaa
      601 ccaccatcag cacgagaatt gagatgccac aggaaacatg aatcatgttg atgagcggcc
      661 gatcgctacg tgggaagaaa ccacgaaact ccattgcgca atacgctgcg ataaccagca
      721 aaaagaccag ccagtgaatg ctgatttgta accttgagta tttattttcc ataacatttc
      781 ctgctttaac ataattttcc gttaacataa cgggcttttc tcaaaatttc attaaatatt
      841 gttcacccgt tttcagtacg ttacgtttca ctgtaagaat ggagattgcc catcccacaa
      901 tcctggtcta agcctggaaa ggatcaattt tcatccgaac gttcctgaca ggagaaaacc
      961 atgagtaaag ttggtattaa cggttttggt cgtatcggtc gactggtgtt gcgtcgttta
     1021 cttgaagtca aaagcaacat agacgttgtc gctattaatg atcttacttc cccaaaaatt
     1081 ctcgcctatc tgctgaaaca tgattcaaac tatggtccgt tcccctggag cgttgatttt
     1141 acggaagatt cacttatcgt tgatgggaaa agtatcgcgg tttacgccga aaaagaggct
     1201 aaaaatattc cgtggaaagc gaaaggggca gaaatcattg tcgaatgtac tggcttttat
     1261 acctccgccg ataaatcgca ggcgcatctt gatgctggtg cgaagaaggt gttgatttcc
     1321 gctcctgccg gtgaaatgaa aaccatcgtt tataacgtta atgacaacac tctggatggc
     1381 aacgacacca ttgtttccgt ggcgtcatgc accactaact gccttgcgcc gatggccaaa
     1441 gccttgcatg acagtttcgg gatagaagtc ggcacgatga cgaccattca tgcctatact
     1501 ggcacccagt cactggtgga tggcccgcgt ggtaaagatt tacgtgcttc acgcgcagcg
     1561 gcagaaaata tcattcccca cactacgggg gcggcaaaag ccattggtct ggtgatcccg
     1621 gaactgagcg gcaaactgaa aggtcatgcg caacgcgtgc cggtgaaaac aggttcggtc
     1681 actgagctgg tgtccattct cggaaaaaaa gtgactgccg aagaggtgaa taacgcactt
     1741 aaacaagcaa ccaccaataa cgagtcattt ggttataccg atgaagaaat agtctcttcc
     1801 gatatcattg gcagccattt cggttcagtg tttgatgcca cgcaaacgga aattaccgcc
     1861 gtgggcgatt tacaactggt gaaaacggtc gcctggtacg ataacgaata tggcttcgtc
     1921 atgcagctta ttcgcaccct cgaaaaattc gctaaactct gacgcgcaca ggcggaggaa
     1981 aaaacctccg cctctttcac tcattaagac tgtaaataaa ccacctgggt ctgcagatat
     2041 tcatgcaggc catgtttacc atctgcgccg ccaataccgg atttacgcca tccggcgtgg
     2101 aaaccttgca tagcttcgaa gttttcacgg ttgatgtaag tttcaccaaa cttcagccct
     2161 ttaatggctt tcatcgcgac gttcagattt tgggtataga ttgatgaggt caggccgtaa
     2221 tcactgtcat tagccattga gatagcatct tccagcgtgt caaatgcgac aactggcagc
     2281 accgggccaa aggtttcctc atgcataatc gacatttcct ggcgaacatc cagcagcaat
     2341 gtcggcggat aataatatcc tttcccctct accgctttgc cacccaacgc cactctcgcc
     2401 ccttcttcta ctgcgcgcgc cactttttgc tcaacccttt ccagcgccgc ggcgttaatc
     2461 aacggcccca tcgcaatgtc gttgcgttca gcggggttac caaattgaac cgcctgcaac
     2521 gcttcaccca gccgattgac gaactgatca taaatgcctt tctgtacata aacacgttct
     2581 gcacagttac acacttgccc actattaatg acgcgtgaat caacgatggc tttgactgcc
     2641 agttcaagat cggcatcgtc cattacgata gccggtgctt taccacccaa ttccagacac
     2701 actttggtga tgtttttcgc cgcagtcgcc atgatcttct cacctgcaga gacgctgcct
     2761 gtcatactga ccattgcgac ctttgggtta cccgccagtt cttgcccaac ggtttcacca
     2821 cgccccagta caaggttaaa cacgccgcgc ggaaggccta tttcatcgac gattttggcg
     2881 aatgcaatcg cattgtttgg cgtaaattca ctgggtttaa tgacgatggt attaccggtc
     2941 aaaagagcgg gagccatttt gcgggcaatg aggaagaacg gaaagttcca cggcagaatg
     3001 ccggtagtca caccaagcgc acgtttaaac agaagaatat tttctcctgg acgatcgctt
     3061 tgaataatct cgccctcgta acgccgtgcc cactccgcca tgtaatcgat atagtcggca
     3121 gtaaaagcca cttcgacttc agctagctgc tggatcttgc ccccttcttc aacaatcagc
     3181 gcactgattt cactggcgcg ttcgcggatc ccggcggaga ttttgcacaa ccaactggcg
     3241 cgttcaatag caggcaacgc ttcccattct ggttgtgcac gttctgctgc atcgattgcc
     3301 ttacgggcat cctcggcctg accatcgggt atgcgggaaa tgacagcctc tgtagcaggg
     3361 tttaccacat caatccatgc gtctccacgc caggtaacaa actgtccatc gatatacata
     3421 ggatgttgaa cgggtactga catgggcgac tcctgtgatt tatatgtttg ttttcggtga
     3481 atatattgtt aattaacatc aatcatcaga gagacggcat cgggaaagga caattctgtg
     3541 gagggcttca taaaaacaag tgtaaaagga ataaaaggca gaaataaaag tcaggagtca
     3601 tgcccgtttg caaacaagtg gttcacaaac gggcaatttt taacgtaacg agcgactttc
     3661 catcgcctcg atgagcaccg catcatgttt cagcgtttgc catgcatgga tgacttctgc
     3721 cggaaaatca acgtgaacga taaaatctcg cccgcgggga ccgtagccat cgctatcatc
     3781 gtgtaaacgc ggcagctcgc cagtgagtag tgagagataa cgctcaaccg gccataatcc
     3841 ttgtaactga ttaataaaga ttacactgtc tgcgttattt cctaactgag gaacgaatcc
     3901 gggataactt aaccagcgtg gtgcatcggg attgtccccg gtcatacggc ggaacgttgc
     3961 catcgtgcgc cgctgcatgg tggggtcctg tacaacgata gccgtatgaa ctcgttctac
     4021 ggcctgattc aatagcgtga tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc
     4081 ttcaatccag attttttcat gcggaatatg ccagaactga tgagcgatat ccacctggat
     4141 ggtcgcttct gctctg
//
LOCUS       DAJVQH010000214         4058 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJVQH010000214 DAJVQH010000000
VERSION     DAJVQH010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.216"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(1..394)
                     /locus_tag="OQM87_004053"
                     /pseudo
     CDS             complement(1..394)
                     /locus_tag="OQM87_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1084..2793)
                     /locus_tag="OQM87_004054"
     CDS             complement(1084..2793)
                     /locus_tag="OQM87_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCS1509965.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(3225..3944)
                     /locus_tag="OQM87_004055"
     CDS             complement(3225..3944)
                     /locus_tag="OQM87_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCS1509966.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1171 a          837 c          745 g         1305 t
ORIGIN      
        1 gcatgctgta cccggtcgta ataaccgctc ctggatacac caggtacccg acacatcgct
       61 tcaacaggga attgttgtcg ccattgttcg actaacgcgt atttttcagc gactcctggg
      121 caaaatacgc tgttgctttt tttaatatat ctcgctcaag tcgtgcttca tttaaagcct
      181 tacgcagttg cataacttca gattccagct cagccactgt gcgggagcct ggtgtattga
      241 gcccttttct tgcggcggta acccattgtc ctaaagtgcc ttcaggaaga gataatcggg
      301 aagcgccttc actgatcgaa agctgatttt caggaaccgt tttgacggct tcggctttaa
      361 actccgtaga gtaacgttgg ttttttctgc tcattattgg ctccttctga tgccattcta
      421 tttcaggaag gagtgtccgt taaactcagg ctacctcaga taagcttgat acaactggtc
      481 tggtatatac agtttctttg agtgcggtta cgtccacaga ggtaacggta aaagtaaccc
      541 cttgtcggga ccactccgac cgcgcacaga ggcattttca tcgctgacag gccgcattgc
      601 ctcgcagggc tggaggagga tcggtgatat ccggacgacc gtaccggctt acgccgggct
      661 tcggcggttt tactcctctg ccatgtgcaa caacggagtg ccgccttaca tgccgctggc
      721 gcggcatatc ctggtaacat ttttgttgat accaccttcc ggccttatcc tgaccagtgg
      781 gctttcctgg catcgatgat accgatgaac gcgcatgata ttgagccgac gatattacgg
      841 gcaactggaa atactcactc tctggatgtg acctttatcc atgaagaaga tttggccaca
      901 ccatggaaac ctgaacaatc atcagtttac gcccatgtaa acccctacgg gatatttgaa
      961 ctcgatatgg aaacaaggtt gccaatcgag gtggttgcct gaaagttggc agttacccgg
     1021 tcactacacc ttgttggagt gaacatatca aaaacaggta attacacaat ttaaattaca
     1081 gtctcataaa aaatgattaa atctatttat tatttcaata aacgtttttt tcatcttctt
     1141 tgaaaattgt tctgctatag tatccggttc cagattgttc tcttttatta ttctacaata
     1201 ctctaatatt gtttctttgt ctctatttac tacagataca aatacaccag gaataccatc
     1261 tttattagtt gcggacagca tttcgaccaa tcgtcttgtg gtaaaactat agttttttag
     1321 aatattacaa aaggcacgta ttgtttcggt atggccatta taaaatgcca taaaaagtcc
     1381 tgatgtaccg ttgggatttt ttatccataa taattcatct atatattcct ggtgaggggg
     1441 gagtattttt agtatatctc catacgccat aatggccttt gtgtgcccat tttgaagtgc
     1501 cataaacaac ccatgcttta ctttcaataa ttctttaatt tgttcactac taagcatttt
     1561 cttattaagc agacttccga acatacgaat actatctgca tgtccattct gcaatgcaaa
     1621 aaataaccca ggtaaatcat ggctgtcttt cgcagctaat agatataatc tcttatcgga
     1681 agttaacggg gttttaaaga ctatttcccc ataggctttt attgtgtcta catgtccgtt
     1741 cataaaagac atgcataaac ccggcacatt gtttgcccct accgcagcaa gcaatgtaag
     1801 tattgtttct tgactaagat tgcacatatt taatatctta ccatatactt gaataatgtc
     1861 tgaatatccg ttttgaagtg ctatataaaa tgcaggagta ccatcagagc ttctcgtggc
     1921 cagaaggttc accagtttag attgagaaat ttcaccattt ttgtattttt ccatcagtat
     1981 aaataatact tctgtcagat tctttgttat cgcatgatat ataactctgg aattgatcaa
     2041 ttcatctggt aaaagtttca ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat
     2101 tacctgcccg ggttgctcca gaccataaat tttccaataa aaatcatcaa aaaaatcctt
     2161 agcggataat tgactcagtt cgaaatggct atcacaagta aatacagtac gtttatgagt
     2221 aacagttcga ttaggatcga aaaactgaac aacccatttt tcactttctt gtacgcgctt
     2281 tattttcaac cctaacgcca tttggtggtt taatgtgcat gctgataatg ttctgacaaa
     2341 gtctccattt tttttcatgt tataaaaaaa attagtaata actgttcccc actttttatt
     2401 ttccacaaaa taaatataag ctggtgattt tgaaaaatct tccgtttttt ccaaacatac
     2461 attttttgca atagcatcag gggtgctaag aagctgatag tttaccttcc catttgtttc
     2521 cataaaattc atttcccaat aggaagctag gtgacggcaa actatttgtc ttccatatat
     2581 atcttttgcc actccattta tatttacctt cacatttctc ttttctgaaa aataaggtgc
     2641 tgttttattc ttaaactgaa ttagttcact ggagtttgcg accctcataa tagaacttgt
     2701 tattgttcta ttatctctgt taacagacat atctgttgat aacgaatttt ttgttgaaaa
     2761 tatactagat gaaaatgttt tatttaacgg catagaagac aaccatagag atgttataat
     2821 actattagat tgcagttact aatagtaaaa gcctccttac aaggaggtag cttattcaga
     2881 atgctcctgt aagatagcga gctcgtcaac gatacagatg atctgattat attgtgcaaa
     2941 aggagaacag gaaaataggc actggaaaag atgaaggcct gatgggagca ttaactaatg
     3001 caaaatccga caggttaata tcccgaaaag caatcaaacg actcaaacag gagatgcacg
     3061 ctgaagtggt ggacaactgt tcggatttca gatgagtagc tcagcagttt atgagccgaa
     3121 tgctctgtca cgggcaggaa tcaggctgca tacgcagtgc caaatctaat cgtagttatt
     3181 ctaaccagtt ttcaagctac aaggtggtgt agctggcatc ttctctactc tatcatcaaa
     3241 cgataaaatg gttcctctcg taacatttgc tcttgccttt cacttccatc tcgatcgctt
     3301 cgtagttcat tacgatagct gacatatttc cagtcttcag gtctaacatc tgacgccgga
     3361 tactccccgg gagcgatttt gcttcttaat agattagact cgagacacat tataaattgt
     3421 cgtattttat gaagtaataa agcactatat tgcgagcatt cctgatctga ttttacatat
     3481 agcgtaaact gagctcctat ccccacacga gattgctgag aaactcgatt catatcagtt
     3541 atcttccatt tatctatagg actgtcctct gaaaataata aaccagacaa aatttggaat
     3601 gccagaggaa cttgctccct agctatacta atatgaaact tgtccccaac aaaatccccc
     3661 ttactctgag gattttcacg acatgcatga ataaacacat ctccactatg gttcacaatc
     3721 tggaatcctt ggtacatgac agaaaaaaat tgacatatat cagagtaagt tggcacgttc
     3781 gatgctactt tgaaatagta cgctgggtat tgccgtattt ggttgtacaa tatattgaag
     3841 ttatttttca atctttcatt cgcagaaagc atctgcggta tttcagacct cacaacattc
     3901 aaagaatcta aatttagttt tagacagggc ttttttatgg gcatagaaaa cgtcctctat
     3961 aaaatagata tattatctta tattgattgt ttatcatctt ttataaaacg atacattata
     4021 tgtaatgtat gtaagcgtaa gggtcttctc cgatcatg
//
LOCUS       DAJVQH010000215         4019 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJVQH010000215 DAJVQH010000000
VERSION     DAJVQH010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.217"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..305)
                     /locus_tag="OQM87_004056"
     CDS             complement(<1..305)
                     /locus_tag="OQM87_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1509967.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
     gene            complement(516..1913)
                     /gene="ccp"
                     /locus_tag="OQM87_004057"
     CDS             complement(516..1913)
                     /gene="ccp"
                     /locus_tag="OQM87_004057"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCS1509968.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2318..3967
                     /locus_tag="OQM87_004058"
     CDS             2318..3967
                     /locus_tag="OQM87_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS1509969.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDLYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVSREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1040 a         1010 c          936 g         1033 t
ORIGIN      
        1 tttacgcaac gcaggtcgat ggctgcggat tgcggatatt cttctttgtc gatccagttt
       61 ttattgatcg acaaatccat caatttatgg acgttttcgt cgtcccaggt ctggcagaaa
      121 gtggctaggt tctgacgagc gttgccatca agatataatt catcattgat aatctggaat
      181 gcgacatcat cgcgcatttc gtgcagcgga aatcgttttg actccgcgat agtagaaatg
      241 gcctttgcgc caaaacgtga atcgagtagt tctgagcgga aatccgttaa cagcttctgg
      301 tccatttcga actccttaaa tttatttgaa ggcaataaaa aagtaggatt tatccgcaat
      361 ggaagcaagg cattacaggc ttaatttaaa taacaaaatc ctaagcagaa aaacgacttt
      421 ttataatata aacatttaac atgataatat taaaaacaga ctttattatc gtaataaaaa
      481 tcgcccaaac attcctgttc gggcgatcaa attaattatt gtttatcctg catatacggc
      541 gtgtacaccc cgttcagact gtgcaggaaa gctacgatat catccacatc ctcctgcggc
      601 agctctttgc ctacctgata gcgcagcatc agtttcaccg ccccgtccag cgtcggcacg
      661 tcaccgcgat ggaagtacgg tgccgttaaa gcaacgttac gtaaaccggg tactttctga
      721 cgcaatttat cacgctcttc tttagtcaca ttcatacgac caatatccgc cgccgtaatt
      781 tccccaaagt taaagtcttt tttcagcacc aacggttcaa aggaacgtcc gccgagaata
      841 ataccaccat gacaagttgc acatttatta tctttaaata attgatagcc ttttttctgt
      901 tgcgccgtca gcgcattttc atctccacgc aaccatttat caaatgggga atccggcgta
      961 attaatgttt tctcaaattc agcaatggca tcagtaatat tttcgccact gaaaccttgc
     1021 ggataaactt cgaggaactg cgctttaagc tgcggatctt tttccagctt agcaataatt
     1081 tcgtcccagg atttcgacgc catttcaatc gggttcaacg gcggtccacc tgcctgatcc
     1141 tgcaatgttg ccgcacgacc atcccagaac tgctcaacgt taaatactga gttaaatacc
     1201 gtcggcgcat taatcggccc aactgcgcca ccaacaccaa tcgatgtttt tctgccatcg
     1261 acgccccccg cattcaacgt atggcaatga gcgcatgaaa tggtgctatc agccgataaa
     1321 cggggatcgt gatacagcgc aaagcccaac gccacttttt gcgcatcggt aggcagtttt
     1381 tgcgggatgg gctgcaccgg ttcattgcga tgctccggag cagtatcatt gctggcgtaa
     1441 tattccgcgc gctgttttgc aatccaggcc agtatttccg cccgctcttc atcactcacc
     1501 ttacccgccc agtgtagcgc ggtataacgc gttggtggca tagtttcata ctgcatcacc
     1561 cattcaatct tattcagatc gctttgcgaa acgggtttat cagccagcag tgccgcacgc
     1621 acggcctcga ggttaaaaga tttatatcca agcttaatgt cgtaatccat caactgtttc
     1681 gcgccaggaa tataataata ggcgggtaat tctgccgaag gcgtgtggca atagtcgcat
     1741 cctttttcgc ggagaaagcc taaaacctta ttattttcac tgacagcaga tgcctgaaca
     1801 tcggcctgtt tactgcgttt attatcgtgg taccacacat aaccagataa ccctaaatag
     1861 caaatcgcga cgccagccag gccgatcgcg gtaatacgtg agaccatttt cattattctt
     1921 ccctcacggt ggtgagttat tgtgacccag aaaattttcc agaatgctgc aaacgtctct
     1981 gcgagacaaa gccatcataa aaaacaggga gatacgcttt ttgataggaa tcaatcaatt
     2041 acatgaatat aaactatcaa taagatagcc tgtgcctatt acccagatca ggaatggcat
     2101 atcatgattg cggcgagagc aggattgaat gttctgaaaa tgaaaaacaa caccaaatcc
     2161 gtatttttat aaattccatt gatattagtg cgttatattt tttgacaagt taataaacgt
     2221 gatccaccgc acgctttgtc gcccaccagg cggagcgaat gactaccctt aaagaaaagc
     2281 ccgataatta gcgacgaatt tcggaggttg gatccttatg ctcaatcaga aaattcaaaa
     2341 ccctaatcca gacgaactga tgatcgaggt cgatctctgc tatgaactgg acctgtatga
     2401 attaaaactg gatgagatga tcgaggcaga accggaaccc gagatgattg aagggctgcc
     2461 cgcctctgat gcgctgacgc ctgccgatcg ctatctcgaa ctgttcgagc atgttcagtc
     2521 ggcgaaaatt ttccccgaca gtaaaacctt tcccgactgc gcacccaaaa tggacccgct
     2581 ggatatttta atccgctacc gtaaagtgcg ccgtcatcgt gattttgact tgcgcaagtt
     2641 tgttgaaaat cacttctggc tgccggaggt ctactccagc gagtatgtat cggacccgca
     2701 aaattccctg aaagagcata tcgaccagct gtggccggtg ctaacccgcg aaccacagga
     2761 tcacattccg tggtcttctc tgctggcgct accgcagtca tatattgtcc cgggcggccg
     2821 ttttagcgaa acctactatt gggattccta tttcaccatg ctgggactgg cggaaagtgg
     2881 tcgggaagat ttgctgaaat gcatggccga taacttcgcc tggatgatcg aaaactacgg
     2941 tcacatcccc aacggcaacc gcacctatta tttgagccgc tcgcaaccac cggtttttgc
     3001 gctgatggtg gagttgtttg aagaagatgg tgtacgcggt gcgcgccgct atctcgacca
     3061 ccttaaaatg gaatatgcct tctggatgga cggtgcagaa tcgttgatcc ctaatcaggc
     3121 ctatcgccat gttgtgcgga tgccggacgg atcgctgctc aaccgttact gggacgatcg
     3181 cgacacgccg cgtgacgaat cctggcttga ggacgttgaa accgcgaaac attctggtcg
     3241 cccgcccaac gaggtgtacc gcgatttacg cgcgggagcg gcctccggtt gggattactc
     3301 ttcccgttgg ctgcgtgata ctggtcgtct ggcgagcatt cgtaccaccc agttcatccc
     3361 catcgatctg aatgccttcc tgtttaaact ggagagcgcc atcgccaaca tctcggcgct
     3421 gaaaggcgag aaagagacag aagcgctgtt ccgccagaag gccagtgccc gtcgcgatgc
     3481 ggtaaaccgt tacctctggg atgatgaaaa cggcatctac cgcgattacg actggcgacg
     3541 cgaacaactg gcgctgtttt ccgctgccgc cattgtgccg ctctatgtcg gcatggcgaa
     3601 ccatgaacag gccgatcgtc tggcaaacgc cgtacgcagc cggttactga cacctggcgg
     3661 gattttggca agcgagtacg aaaccggtga acagtgggat aaacccaacg gctgggcacc
     3721 gttacaatgg atggcaattc agggatttaa aatgtatggc gatgaccttc tgggtgatga
     3781 aatcgcgcgc agttggctga aaacggtgaa tcagttctat ctggaacagc acaaaatgat
     3841 cgagaaatac catattgccg atggtgtttc ccgcgaaggc ggcggtggcg agtatccgtt
     3901 gcaggatggg tttggctgga ctaacggtgt ggtacgccgt ttaattggtt tgtacggcga
     3961 accataatat ttttacagcc agccgctaac ttcctgctgg ctgtaaaatt atcctcttc
//
LOCUS       DAJVQH010000216         3988 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJVQH010000216 DAJVQH010000000
VERSION     DAJVQH010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.218"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..213)
                     /locus_tag="OQM87_004059"
     CDS             complement(<1..213)
                     /locus_tag="OQM87_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCS1509970.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OQM87_004060"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OQM87_004060"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCS1509971.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OQM87_004061"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OQM87_004061"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCS1509972.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OQM87_004062"
     CDS             2894..3634
                     /locus_tag="OQM87_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1509973.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..>3988
                     /gene="ruvC"
                     /locus_tag="OQM87_004063"
     CDS             3669..>3988
                     /gene="ruvC"
                     /locus_tag="OQM87_004063"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCS1509974.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQV"
BASE COUNT          980 a          988 c         1120 g          900 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtctt acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggt
//
LOCUS       DAJVQH010000217         3836 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJVQH010000217 DAJVQH010000000
VERSION     DAJVQH010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.219"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(74..931)
                     /gene="sitD"
                     /locus_tag="OQM87_004064"
     CDS             complement(74..931)
                     /gene="sitD"
                     /locus_tag="OQM87_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCS1509975.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(928..1785)
                     /gene="sitC"
                     /locus_tag="OQM87_004065"
     CDS             complement(928..1785)
                     /gene="sitC"
                     /locus_tag="OQM87_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCS1509976.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1782..2609)
                     /gene="sitB"
                     /locus_tag="OQM87_004066"
     CDS             complement(1782..2609)
                     /gene="sitB"
                     /locus_tag="OQM87_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCS1509977.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2609..3523)
                     /gene="sitA"
                     /locus_tag="OQM87_004067"
     CDS             complement(2609..3523)
                     /gene="sitA"
                     /locus_tag="OQM87_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCS1509978.1"
                     /translation="MHSIKKVTMLLGCLALTCSITFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1048 a          958 c          926 g          904 t
ORIGIN      
        1 ctcctcccaa cgaccttcca tggctttccg gacttctgta ggctggcgga cgatgctgtc
       61 ctcggatgtt gtgctaattg ggtgataaca gatcctgtgt atgggcgttt ccctttgtgc
      121 gtgcgttgat gctggtaacg gtaaacgtca tgatgaacac gaccgcgaat aagacgacaa
      181 tcgtcggagc tggtgcgctg tcgataaaaa aggacagcca gacaccagtc attgacacta
      241 ttaccgatat gccagtcgcc agtaggagcg caatatgaaa gcgttgtgtc aggagtactg
      301 caatggcgcc aggcgcaatg agcagagaaa tagagagaat gatacctact gctttcagcg
      361 tcgccacaat ggtcagggaa accatgcaca gcagcccata atgcagccat ctcgtatgca
      421 aaccgcttac ctgcgcctgt tgataatcaa agctgaacag caaaaaatcc cgccatttta
      481 cgctaataac aagtgtaacc agcccggcaa taatcaccgt ctggattata tcgccgatgg
      541 ttatcccgag catgtcgccg aaaagaatat ggtcaagatg tacgtctggt ttaattgcaa
      601 tatacaagat cagacctgcg gcaaacatgc cagaaaaaac aatccccata actgtatctt
      661 gcttgattcg gctgttgtct ttcaggtatc ccgtcgccac cgcgcagaat actccggcga
      721 cgaatgcccc tgtagccaga ggtaacccca gaatccaggt aagcacgatg ccaggaaaaa
      781 ccgcatgact catcgcgtca cccatcagtg cccaaccttt caggaccaga aagaccgaca
      841 gcagcgcaca gggtatggaa actaccagtg aaatcagcag tgcgtgactc ataaaggtga
      901 actgcagtgg ctccagtaat aatgccatca tgagcgatcc tccagcgctt tatgcgcacg
      961 ccgacgattt gccaaaagtc catgggtggg cgcaaataca aatgctaaaa gaaacagcag
     1021 tgtctgcgcc accacgatga ttccacctgt cgcgccgtcc agaaagtaac tgacccacgc
     1081 gccgaggaaa ctggttacac tgccaatagt tacagcaatt atgagcaggc ggggaaaacg
     1141 atcggttaac aaccatgctg ttgcaccggg agtcaccacc agacagatca ccagaaaggc
     1201 accaaccgtt tgcagcgccg caacggtcga aaccgacagc agggtgaaga agaggatttt
     1261 taatctgccc ggatgtaatc caatagcacg ggcgtgattc tcgtcgaaaa aggtcaccat
     1321 aagatctttc catttgaaaa aaagcacgat gatcgagaga atgccaatga tagtgagttg
     1381 caggatatcc gccggatcga ttgccagaat attgcccagg acgatggtct gaatgttcac
     1441 ggatgtcggg ttcaatgaca ccataaacag ccccaggcca aaaaaggacg agaagatcag
     1501 gccgatgatg gcatcttctt ttaagcgggt gcgttggtta agaaacagca tgctgcccgc
     1561 tgccagtccg ccggaaaaga aggctcccag agaaaacgga agccccagca tatatgcccc
     1621 cgcaacaccc gggacaatag agtgcgacag cgcatcgcca atcagcgacc agccttttag
     1681 catcagatag catgacagga aagcgcagag accgccgacc attgccgaga cccacatcgc
     1741 attgagcata tattcatagc tgaagggttc cagtagcacg ttcatcttgc ttccctctgc
     1801 accgctgacg gtcgatgtgc cacaaaaggg cgttcatcgt cagtaataat gctttcttcc
     1861 gagccgttaa gtgtgacatg gcgcagtacg ccgctaaaag ccagctccag gttctcggct
     1921 gtaaaagtgg tgtctgtcgg cccgcttgcc aggacggtgc ctttgaccat gaccgtataa
     1981 tcgcaaaatg tcgtgactga gccgagattg tgcgttgaga caagcatcgt tttgccttcc
     2041 gcgcgtaact ccctcaacag gctgatgatt ttcgcttcgg ttttcacatc aacgccggta
     2101 aaaggttcat ccagcaggat cacatcaccc tgctgtgcaa tcgctctcgc cagaaagacg
     2161 cgtttctttt gtccaccgga aagctcgccg atttgacgat ggcgaaaatc caccatatcg
     2221 acacgttcca gcgcatcggt gacaatctga cgatccctct ttttggctat acgcaacata
     2281 cccatatgcc catagcggcc catcatcacc acatcttcca ccagtacagg aaatgaccag
     2341 tcaacctctt ctgactgagg gacgtaggca accaggtttt tctgtagcgc ctgtcgcgtg
     2401 ggaataccca gaacagatat ctttccgctg gtcagacgca caaatcccat aatcgcttta
     2461 aacaacgtcg attttccgga accgtttacc ccaaccagag cggcaataga tccacttggt
     2521 actgtgaagg atgcatcgcg cagtgctgtg tgcccgttac gccaggtgac agtaacatca
     2581 ttaacgacaa tgcctgcaga ttgcatcatt atttctccct tttcccggct ttaattccct
     2641 gtaccagcgt actggtagta accttaagaa ggtcgatata cgtaggtacc gggccgtttt
     2701 ctgtgctcag ggaatcgaca tagagcacac cgccgtagtg tgcgccggtt tcacgcgcaa
     2761 cctgacgcgc tggtttatcg gaaatcgtac tctcgctaaa gactgccggg atatgatttt
     2821 tcttaactat atcaacaacc ttacgtacct gctgcggtgt tccttgttga tcggcattaa
     2881 tcggccacag ataaagctct tttagcccca aatcgcgtgc gagataagaa aaagcccctt
     2941 cactggtgac catccatcgc tgattctcag ggagttccgt aatctgctta cgcagggggg
     3001 caagggtttg ggtaatcttg gctttataag tatcggcatt acgttggtag gtttgtgcat
     3061 ttgccggatc gtattttatc agcgcatcac gaatattatc gacgtaaatc agagcattat
     3121 ctggcgacat ccatgcatgg ggattaggtt tgccctcata gggtccttcg gtgatcccta
     3181 ctggcgtcac acccgaagag acaattactt ctggaacccc attgagatgc tggtaaaagc
     3241 gttggaacca caattccaga ttcataccat tggcgagaat cagttgtgcc ccctgcgcac
     3301 gtttaatatc gccaggggta ggctgatact catgaatttc tgcaccaggc ttggttatgg
     3361 atgagacttc tgcagcatct ccagccacgt ttttggccat atccgcgatg atggtaaatg
     3421 ttgtaatgac cttgaatttt tcagttgcac ttgcctgaaa tgtgatcgag caggtgaggg
     3481 cgaggcaccc caagagcatg gttacttttt ttattgagtg cataatttag tacctatagt
     3541 gagcaaaatt agaacaaaca tagcaacggc tatgttttat agcataacct atacaaaatg
     3601 aaaataatta tcattaacag ctttgaggcc taaaacaggg attaggttgt ttcctgctgg
     3661 cgacatgata ttgaggaaaa aagtagagca gctattaggc tttgaaatga ctttatgagg
     3721 aaaattgttg atggcaaaaa tggggcaaaa cgctgtataa ggggcaaaaa aggggcaaaa
     3781 aagagtgggt tatcgtagct tattgttgtc gctgataatg tttaacatat tgaaaa
//
LOCUS       DAJVQH010000218         3834 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJVQH010000218 DAJVQH010000000
VERSION     DAJVQH010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.220"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1048..1488
                     /locus_tag="OQM87_004068"
     CDS             1048..1488
                     /locus_tag="OQM87_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509979.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(1567..3264)
                     /gene="treA"
                     /locus_tag="OQM87_004069"
     CDS             complement(1567..3264)
                     /gene="treA"
                     /locus_tag="OQM87_004069"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS1509980.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(3584..>3834)
                     /locus_tag="OQM87_004070"
     CDS             complement(3584..>3834)
                     /locus_tag="OQM87_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="phosphotransferaese subunit; phosphorylates
                     dihydroxyacetone along with DhaK/DhaL; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCS1509981.1"
                     /translation="PQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALI
                     ARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         1001 a          852 c          912 g         1069 t
ORIGIN      
        1 caaaatcgat atatactttc atatatatcg ataaaaatca tgttcaatat caaagtttag
       61 ctattgaaaa catgaagatg tgacgacttc cgcaagcctg ttgaccctct ctatcattaa
      121 aaatgtattt atcaggtggc taatattatt taaatcattt aaggctaaat catttaaata
      181 tgaatgacat tattgttttt tatcgtccac ggttacgcga cgttgaagtt acttgtttta
      241 caatgagcga tttttgatat ctcgccgaga aaattatcag cattctctcg tttcagtgtc
      301 gtgaatatac tgttaacgga ggtgaaaaga atgctggggg ttatacaaaa cctatgttgt
      361 tgtcgtccgg attcaccgct tgctgatagt aagcagcgta accagaagtt accgcaacaa
      421 gactttgagg attttaagaa cgacgttatt aatgtcatta acaacaatga agatatttat
      481 tttcttagag agagtaattt actgaatgcg tttattcgaa cgtgcaaaaa aagagatgat
      541 aacagttacg gaaatatgat atttacgctt tattttgata acgatatcta tgggaacaga
      601 attatcaaag catcgctgaa cggcggagag ttgcagttat taagcacagg gtgtagcaat
      661 gataacattc tccattttat taatgcattt ctcattcggg tgaaatttcc gctacctgga
      721 cgcataaaac cggaactctc gccaaaagcc gggcactccg aaggaattga cgcgttgcgc
      781 tgctctgtaa cggcatttac cgcaagccat ttatttcgcc agcgacaatc cgcagagaac
      841 caaacgcatc aggaaacgac tgaacacaat aaatactgta ttgatcaaca tattatactg
      901 gcacctccag cacgcagtga ggatacactg tatcatcagc gaatcttatc actgcggagc
      961 tggttgaacg actttctgag cgaaataagt cggttcccgg taacaacaac agaatccttt
     1021 tttaattatt gtttcgttgt ttttggtgtg attcagaaaa taaatatttt agacagtagc
     1081 cagtcaggcg ttaaaaatgg ggcggatagt gaggcggttt tggctgcgct tattgaacat
     1141 ttagaattga tcaatccttc tggcagatta agccaaaata cccgttcggc aatgcttcag
     1201 ttacgcgaag aggaatggtc tgaattcttt ttttggttgc taaattctct ggagtgtctg
     1261 gattatgtga ttattaattt aactccagaa agcaaaaaaa cgttgatgag tgaacaccgt
     1321 aacaatattc aggtagcaat tgatgcttta tatagccaac gtcggcgtaa atcgccgggt
     1381 gatgaaagtg aaacacttac ccgtagaaat gacgctatct tcggcaacca cgtatggcaa
     1441 acatttgcgc aatacttccc gccaggactt gaaaaaccgt ctgtgtagaa aaaaccatag
     1501 atatggtgtt atctgccgac aacgccatcc ggagaagccg aatgacgttg tcggagtaag
     1561 cgctggttaa ggtgtgggtt gtgcctcttt ggttgagggt tgcgtcgttg ctgacttaac
     1621 ggtcggacgc gtcgccggaa cattgtcaca cggttgctct ttcgggcaga tcaaatccag
     1681 cattttcagc gtcacgccat tggtccagcc aaagccatcc tgtaatggat attcgccacc
     1741 gccgcccccc gttccggtgg tgctgacatc atatttttcc accagctttt tctcccggtc
     1801 ataggtgtgc tgaacattgg tcaggaagtg ccagctaatg tccatcgcca cctctttttg
     1861 cccgtagttt tgtaatcctt ctgtcgcgac ccactgtaac ggtgcccagc catttggcgc
     1921 atcccattgt tgcccacttt tcaccgacgt ggtgttcagg ccgccgggtt gcagcagatg
     1981 tgttttcgtc gccgtcgcca ttttgttggc gcgatctttc gctgccgcat tgacgtacag
     2041 cgggaacagg gcggccgcgg ttaactgatt gcgcacttta tgacttttca ggtcgtaatc
     2101 ggcataccag ccttgttgat cgttccacag gtatttttcg atcccttttt gacgggcatt
     2161 cgccagcgtt tcgtactggt ttgccatcgc gttatctcct gcagctttgc tggcgcgggc
     2221 gaggattttt tccattttaa acatcaggct gttcagatcg accggtacga tgctggtggt
     2281 gcgtaaggta tttaactgct gcgggttgtc catccagcgc gagctgaaat cccagccaga
     2341 cgcagcggca gagcgcaggt cgcggtaaat ttcagtggca ggtcgattcg gattgctttt
     2401 ggcggtggca atatcttcca cccatgactc tggtcgtggc gtatcgcgat cgtcccagta
     2461 gcggttgaga agggtaccat cctgaagttt tacaacgcgt ttttcctgtt gtccggcttg
     2521 caggttttca acaccgtcca tccagtaagc atattctttt tgcatttgcg gcaggtattg
     2581 cttcaacgcg gcatcgcctt catgctgcgc cagtaactct accatcaggg caaagaaggg
     2641 cggttgcgag cggcttaaat agtaactgcg gttgccgttg ggaatatgac cgtaagtgtc
     2701 tatttcatga gcaaaattgg ccaccatatc cgcgacttta tcccagtgac cgctttcggc
     2761 aagtcctaac atggtgaagt aactgtccca gtaatatacc tcgcgaaaac gtccgcccgg
     2821 cacgacataa ggttccggca gcggtaacag agaatcccat ttttcggtgt tttcggtaga
     2881 acgcgttaat accggccaaa gtccgtcaat atgttcgcgc agtgactgcc cctctggcgg
     2941 aacatatttc tcgccttctt tcggcagggt gaaattgacg ttaacgaaat ggcgcagatc
     3001 aaatccgctc tggttttgct gcatccgata atcagcaagg atcatcagcg gatcgctgtt
     3061 cggcacggca tcggcaaagg ttttttggtc cggaaaaagt ttggcgtttt gcacatcatt
     3121 aaacagcggc cctaataaaa tatcaggcgg ctgtggtgtt accggtgttt cttctgcctg
     3181 caccgatagc gcagcgaaac acaaaaagat acaggctgga attaacgcca ttttttgcgg
     3241 gcgagaaggt gcgggggatt tcatcaatca ttctcctttg gcgaaaccga ataaaacggt
     3301 attcaagtat cagaaaacct tagttcagga tcgcactagc tgcatgatcg aaatctcatt
     3361 ctgcgaacgg tcaggcattt tagaaaaggg aattaaaggg ataaataacg gcgaaataga
     3421 gtgatcaaat aacgtctgtt tattagccag ccagcgacat ttggttcgcg attagcgaag
     3481 aaaaatcatc ataaattggt aaaaaatata acaggtagtt aaaaaacatc agtgctgggt
     3541 aaattgccgg atgacatcag aacgatgcca tccgaacggt ggcttaaccc tgacggttga
     3601 aacgttgcgt tttaacgtcc agcgttagcg tttcttctgg ttgtatcgca tacagtttct
     3661 caccctgctg gcaaatccag ccaatcccca gttcacgggc gattagggcg ctgtgggata
     3721 ccggacttcc ggcgctaagg cagatacctt ttacaaccgc cggatccagt tgcagtactg
     3781 tggaaggata aatgttctcc gccagtagaa tagttggcga gttaaactgc ggga
//
LOCUS       DAJVQH010000219         3710 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJVQH010000219 DAJVQH010000000
VERSION     DAJVQH010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.221"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            80..2077
                     /gene="betT"
                     /locus_tag="OQM87_004071"
     CDS             80..2077
                     /gene="betT"
                     /locus_tag="OQM87_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCS1509982.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2140..3417
                     /locus_tag="OQM87_004072"
     CDS             2140..3417
                     /locus_tag="OQM87_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCS1509983.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          897 a          842 c          937 g         1034 t
ORIGIN      
        1 atagccagtt gaactttgct actttgtgag aggtagtacc ttctatccag tgcgaattta
       61 attaatggaa taaatgatta tgagtgaaaa tgatacaatc ccaaagaagt ctacaagtca
      121 gattaacaaa gcggtattct ttacatctgc tttgctaatt ttccttcttg tcgcctttgc
      181 cgccgtattc ccggatgtcg ccgacaaaaa ttttaaacta cttcagcaac aaatcttcac
      241 gaatgccagc tggttctaca tccttgctgt ggccctgatt ttactgagtg tcacgttcct
      301 tggactctca cgctacggtg atatcaagct gggcccggac catgcgcagc ctgatttcag
      361 ctaccactcc tggtttgcga tgcttttttc ggcagggatg gggatcggcc tgatgttctt
      421 tggcgttgcc gaacctgtaa tgcattatct ttcgccaccc gttggcactc cagaaaccgt
      481 tgcggcagct aaggaagcaa tgcgtctgac ctttttccac tggggactgc acgcatgggc
      541 aatttatgcc attgtggcgc tgattctggc gttcttcagt taccgtcacg gtctgccttt
      601 aactctgcgc tccgcactct atcccattat tggcgatcgc atatacggac ctgtaggaca
      661 tgcggttgat attttcgctg ttataggcac ggtctttggc gttgcgacat cactgggtta
      721 cggtgttttg caggtgaatg ccggtttgaa ccatcttttc ggggtgccca tcaatgaaac
      781 ggtgcaggta attctgatcg tggtcatcac ggggttagcg acgatttcag tggtgtccgg
      841 tctggataag ggaatacgta tcctgtctga actcaatctg ggtctggctt tgttgctcct
      901 ggcgctggtc ctgtgtctgg gaccaaccgt gcttctgctg aagtcatttg tggaaaatac
      961 gggcggttat ctttcggaac tggtgagtaa aacgttcaac ctttacgcgt atgagcccaa
     1021 gtcgagcaac tggctggggg gctggacatt actgtactgg ggatggtggc tttcatggtc
     1081 gccgtttgtg gggatgttca tcgcacgggt ctcccgcggg cgaaccattc gcgagtttgt
     1141 caccggcgtg ctgtttgttc ccgcgggttt tacgctaatg tggatgacgg tgtttggtaa
     1201 cagcgcgatc tatctcatta tgaaccaggg ggccacagac ctcgccaata ctgttcagca
     1261 ggatgtggcg ctggccctgt ttaatttcct ggagcatttc ccgttctctt ctgtgctgtc
     1321 attcattgca atggcgatgg tcatcgtctt ctttgtaacg tctgctgatt cgggggcaat
     1381 ggttgtggat actctggcat caggtggagt ggcaaacaca cccgtctggc agcgaatatt
     1441 ctgggcctcg ctcatgggca ttgttgcaat tgcgcttctc cttgccggag ggctaagtgc
     1501 gctgcaaacg gtgacaatag cgagtgcatt gcccttctca gtgatcttac taatatctat
     1561 atacggactt ttaaaagctt tgcgccggga tttgaccaag cgtgaaagcc tgagcatggc
     1621 gacaattgct cctacggctg cacgtaaccc aattccttgg cagagaaggt tacgcaatat
     1681 cgcgtatctg ccgaagcgat ctcttgtgaa acgttttatg gacgacgtta tccagcctgc
     1741 catgacgctg gttcaggagg aactgaacaa gcaggggacg ataagccaca ttagtgatgc
     1801 agtcgacgat cgtattcgtc ttgaagtcga tttgggcaac gagctgaatt ttatatatga
     1861 agtgaggctt cgcgggtata tctcaccgac cttcgcgctc gccgcaatgg ataatgatga
     1921 gcagcagact gaacaacatc gatattatcg cgctgaggtt tatctcaaag aaggcggtca
     1981 aaattatgat gtgatgggct ggaaccagga acagctgatt aatgacatac tggaccagta
     2041 cgaaaaacac ctgcacttcc tgcacctggt tcgttaatag caacatgccg tcctgggggc
     2101 ggcaattatt atcccggccg caatatgagg gaatgcagaa tgatttcacg ctggaaatgg
     2161 atgctgaagc agacaattaa aaaactatgg ttcagggcaa cgttattcgc aattgtcgcg
     2221 ataataacgg cccttttatc aattcttttt aaatcaatga tacctgagtc ggtttccgtg
     2281 aaggttggtg cggaagcagt cgataacatt ctgaacatac tggcatcgag tatgctggca
     2341 gtgaccacat tttcgctgag tatcatggtc acagcctacg gttcagccac tactaatgtg
     2401 actcccagag ctacgcgttt agttgttgaa gacgtcacca cacaaaatgt actggccacc
     2461 ttcatcggtt cttttctctt cagtctggta gggattattg ccctcaatat gggagcttat
     2521 ggagaaaggg ggagagtcat tttattcatt gtcacactgg ttgtcattgc cttaatcctc
     2581 atcacattgc ttcgctggat acagcatttg acctctctgg ggagggttgg tgagacaacg
     2641 gcacaagtag aacaggcggc catcgaaaca tttattgcga gagcaagaaa tccctgtctc
     2701 ggtggatatc catggcttga gaacaatgaa cagccgaaag gaacggttgc agtttatccg
     2761 aagaagattg gctatgttga atatattgat atggtgaaac ttagcaagct gctgaccaat
     2821 gatccccgtc atgtatacct cgtggcgcag ccaggcagtt tcatacatcc gtccatgcca
     2881 gttttgtacc tgagtcaggg ccaggagtcg tcaatcaaca ccgatttact tgagacgatt
     2941 attgtctcgg atgcacgttc atttgctcag gatcctcgat tttgtcttag cgtcatggcc
     3001 gaaatagcct gccgggccct ttcccctgca gtgaacgatc ctggaaccgc cattgatgtc
     3061 attggcagag gtgttcgtat actttccact tacgcgcaga ataaatctga tgaaatagaa
     3121 gtgaaatatc cttcagtaca tgttgcacca cttcagaata acgatctact ggaagacttt
     3181 ttctcacctg tcgcgcgcga tggtgccagt atgagggaga ttcagataag agtccttaaa
     3241 ggactgtcga tgctgagtaa aggttggcct gagatatttg ctgaagccgc acataccctg
     3301 gcatttgaaa cattagagca tgcaactcgt gctgaccata tagattctga tagatatcta
     3361 ataaaatcaa cttattataa tttatttagt ggcgaatatt ctaataaaaa aacatagctg
     3421 cagagcaagc aattcggttg ttggcaagtc aagggcaaag cattcgcgtg agaatgggcg
     3481 tatgctttgt gccaaaagca aacgttgcta attctgcact gttcagcgat ggcgcagtag
     3541 agacaggcac tgccactgtc cagtcggcaa aagcgaacac tttgccactg cggggataaa
     3601 ctgattgaag ctctcgcgca aaatcactta ttcttttgcg ttaataaaat gtaggagatg
     3661 ggtcattgat cctacccacg taatatggac acaggcctaa gcgaggttct
//
LOCUS       DAJVQH010000220         3687 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJVQH010000220 DAJVQH010000000
VERSION     DAJVQH010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.222"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..222
                     /locus_tag="OQM87_004073"
     CDS             <1..222
                     /locus_tag="OQM87_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCS1509984.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            268..477
                     /gene="hha"
                     /locus_tag="OQM87_004074"
     CDS             268..477
                     /gene="hha"
                     /locus_tag="OQM87_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1509985.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            516..1106
                     /locus_tag="OQM87_004075"
     CDS             516..1106
                     /locus_tag="OQM87_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCS1509986.1"
                     /translation="MKLIIFILIVLIIATLLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1346..1606
                     /gene="repA"
                     /locus_tag="OQM87_004076"
     CDS             1346..1606
                     /gene="repA"
                     /locus_tag="OQM87_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCS1509987.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1830..1904
                     /gene="tap"
                     /locus_tag="OQM87_004077"
     CDS             1830..1904
                     /gene="tap"
                     /locus_tag="OQM87_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCS1509988.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1897..2754
                     /gene="repA"
                     /locus_tag="OQM87_004078"
     CDS             1897..2754
                     /gene="repA"
                     /locus_tag="OQM87_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCS1509989.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3454..3645
                     /locus_tag="OQM87_004079"
     CDS             3454..3645
                     /locus_tag="OQM87_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509990.1"
                     /translation="MTENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          992 a          901 c          932 g          862 t
ORIGIN      
        1 tttcagcgag atcgttatgg tcgtattctc gggcaggttt atgcaccaga cggaatgaat
       61 attaatcagt ttatggtccg ggccggggct gcctgggtat atgagcagta caacacagat
      121 cccgtgctac ctgtccttca gaacgaagcc agacaacaaa aacgtgggct ctggtcagat
      181 gctgatccgg ttcccccctg gatatggagg caccgtaaat aaagcgatgg caatcctatc
      241 attcatctgt tctcagcggg aataaaaatg gcaaaaacaa aacaggaatg gctgtatcag
      301 ttacggcgat gttcatcagt caatacgctg gaaaaaatta ttcataaaaa cagagacagt
      361 ttatcaaaca gtgaacgtga atcatttaat tcagcagcag accaccggct agcggaatta
      421 atcaccggaa agttatatga caggatacca aaagagatat ggaaatatgt aaggtaataa
      481 ttcagcaata attaattgcc aggagcagat aaataatgaa gttgattatt tttattctca
      541 ttgttctgat tattgcaacg ctacttatcc ggattatcct cagaagtgta aatcagcatt
      601 ctcctttgct gatgcaattg catgcggcag gtatacggac tggtgatgca gagcgaatac
      661 tgtccagcgg tgaatactgg caacgtcaga agacgctgct gacagaaagg gaagtcagtt
      721 ttatgaaagg actgttcaga attgtggata tgaagcggtg gtatctgtgt ccgcaggtac
      781 gggtcgcgga tatcgtccag ctgaacggga atatccggcc acgatcacgc cagtggtggc
      841 agttattcag gatggtgtct cagtggcatg ttgatgtggt tatcgttgag ctgcgttcgt
      901 tcagtattgt tgcagcagta gagctggatg atgccagcca tttacgaccg gaacgcagac
      961 gccgggatat tcttctggaa gaggttctga ggcaggctgg tattccgttg ctcagaagcc
     1021 acgatgccag aaaactgctg cagatgaccg gagaatggct gaatacaaca ggggctgatc
     1081 agcagtcacc ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc aaccccggaa
     1141 acgggaaaaa gcaagttttc ctcgctcccg gcgtttccat aactgaaaac catactattt
     1201 cacagtttaa atcacattaa acgacagtaa tccccgttga tttgtgcgcc aacacagatc
     1261 ttcgccacaa ttctcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc
     1321 cctgtttgag tattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatt
     1381 gagtcaaaag cggtttgtgc gcagaggtaa gcctatgact gactctgaga aacaaatggc
     1441 cgctgttgca agaaaacgtc ttacacacaa agagataaaa gtttttgtca aaaatcctct
     1501 gaaagatctc atggttgagt actgcgagag agaggggata acacaggctc agttcgttga
     1561 gaaaatcatc aaagatgaac tgcagagact ggatatacta aagtaaagac tttactttgt
     1621 ggcgtagcat gctagattac tgatcgttta aggaatttta tggctggcca cgccataagg
     1681 tggcagggaa ctggttctga tgtggatgta caggagccag aaaagcaaaa accccgataa
     1741 tcttctttaa ctttggcgag tcagaaagat taccggggcc cacttaaacc gtatagccaa
     1801 caatcaagct atgcggggag tatagttata tgcccggaaa agttcaagac ttctttctgt
     1861 gctcgctcct tctgcgcatt gtaagtgcag gatggtgtga ctgatcttca acaaacttat
     1921 taccgccagg taaagaaccc gaatccggtg ttcacacccc gtgaaggtgc ccggacgctt
     1981 ccgttctgcg ggaaactgat ggaaaaggcg gtgggcttca cctcccgttt tgatttcgcc
     2041 attcatgtgg cacatgcccg ttcgcttggt ctgcgtcggc gcatgccacc ggtactacgt
     2101 cgccgggcca ttgatgcgct gctgcagggg ctgtgttttc actatgaccc actggccaac
     2161 cgcgtccagt gttccatcac cacgctggcc attgagtgcg gactggcgac ggagtctgct
     2221 gccggaaaac tctccatcac ccgggccacc cgggccctga cgttccttgc agagctggga
     2281 ctgattacct accagacgga atatgacccg cttatcgggt gctacattcc gaccgatatc
     2341 acgttcacat ctgcactgtt tgctgccctc gatgtatcag aggaggcagt ggccgccgcg
     2401 cgccgcagtc gtgtggaatg ggaaaacaga cagcgcaaaa agcaggggct ggataccctg
     2461 ggtatggatg aactgatggc gaaagcctgg cgttttgttc gtgagcgttt ccgcagttac
     2521 cagacagagc ttaagtcccg tggaatgaag cgtgcccgtg cgcgtcgtga tgcggacagg
     2581 cagcgtcagg atattgtcac cctggtgaaa cggcaactga cgcgtgaaat ctcggaaggg
     2641 cgcttcactg ccagtcgtga ggcggtaaaa cgtgaagtgg agcgtcgtgt gaaggagcgc
     2701 atgattctgt cacgtaaccg taattacagc cggctggcca cagcttcccc ctgaaagtga
     2761 cctcctcaga ataatccggc acgcgccgga ggcattcgca cgcctgaagc ccgccagtgc
     2821 acaaaaaatc agcgctacat acaaaaaaca acctcatcat ccaccttctg gtgcatccgg
     2881 tcccccctgt ttttgataca aaacacgcct cacagacggg gaattttgct tatccacatt
     2941 aaactgcaag ggacttcacc gtaaggttac aaccgttcac gtcataaagc gccagccgtc
     3001 aaccctacag ggtgcaatgt atcttttaaa cacctgtgta tatctccttt aaactactta
     3061 attacattca tttaaaaaga aaacctgttc actgcctgtc ctgtggacag acagatatgc
     3121 acctcccacc gcaagcggcg ggccccaacc gaagccggtt tagttacaac atgcaaacat
     3181 aaacccctaa aaaacccaag tccaacgcag aactgaaacc acaacgcccc tcctcataac
     3241 tgaaaagcgc cgccgccccg cccgaagggc gggaacagag tcgcttttaa ttatgaatgt
     3301 tgtaactaca tcatcatcgc tgtcagtctt ctggctggac gtatcgagta cacgctcgta
     3361 agcggccctc acggcccgct aacgcggaga tacgccccga cttcgggtaa aaccctcgtc
     3421 gggaccactc cgaccgcgca cagaagctct atcatgactg aaaacgggta tggcttagca
     3481 gggctgggga tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc gggcttcggc
     3541 ggttttactc cggtatcaca tgtaaccaca gcgtgccgcc ttccatgccg ctggcgcggc
     3601 atcagaataa tacaagcagc atttaatgca tttgcaaaag tgtgagtaat attaagtggt
     3661 cgtttcaggg atatcgttaa gacttga
//
LOCUS       DAJVQH010000221         3682 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJVQH010000221 DAJVQH010000000
VERSION     DAJVQH010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.223"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(171..662)
                     /locus_tag="OQM87_004080"
     CDS             complement(171..662)
                     /locus_tag="OQM87_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1509991.1"
                     /translation="MLFNNQDWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRVEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(983..1711)
                     /locus_tag="OQM87_004081"
     CDS             complement(983..1711)
                     /locus_tag="OQM87_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1509992.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKMRGILIA
                     FTGTDQQKQEHLLKLAQSGKVEGI"
     gene            complement(2674..3327)
                     /locus_tag="OQM87_004082"
     CDS             complement(2674..3327)
                     /locus_tag="OQM87_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HCS1509993.1"
                     /translation="MAEYYPAEFGGTAFNCPNCGAYSQQRWVRLKYGANGIYETPFAF
                     SKCERCNEKAYWYDETMIIPASANVEMPNPDMPDDCKSDYMEARSIVNLSPKGAAALL
                     RLSLQKLMVHLGEPGKHIDTDIKSLVAKGLSPLVQRAADICRIVGNQAVHPGEINIDD
                     DPQLAHGLFKLLNIIVTEQITRPKEVEAMFNSMPERALKGIEDRDRKAREQQQAASE"
     gene            complement(3320..>3682)
                     /locus_tag="OQM87_004083"
     CDS             complement(3320..>3682)
                     /locus_tag="OQM87_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1509994.1"
                     /translation="FVLADFISLVEDRRKIEGDNAMVFDGLKLMKNGYGEQVSKWFNR
                     TLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITYDRYAKEVEV
                     DRLVNVMAGVYKETGVNG"
BASE COUNT          885 a          930 c          919 g          948 t
ORIGIN      
        1 ggcagacctt cattgacctg aatgttgtcg gtgtcggtga tacgtactgg ctcaataccg
       61 aactggaaag cgtctttaat cgtcataata atcaatctcc taaattagtt taaatactgt
      121 gagtcctcaa aactgaggag tccctagcat cgggtcaggg actgtgtgtt ttacaggctg
      181 tccatccagt taccgcctga ctcaaggtca gcgacgatag cggcacggga gcggccagcg
      241 gaatgggtag cactgcggag gttttcacca taggtccggc gttcagcttt gacctgttcg
      301 cggcgctgta gctctttctg ccattcgtct tcggtcatga acatttcgcg ggtacgctct
      361 ttctgagacg ccagctcttt agcgatacgc tcagcctcga cacgcttatt catctggcgg
      421 cgttggtagg cggtcatcag gtcattactg aagaagttac caatggtcac gccagcttca
      481 gccagagctt ccagtaactc cattgcctcg cggctggtag acggcaggcc tgacaggtag
      541 cccggaatga gggtcttgat agcgagcgac agcggatagg gctgaccgtc cagactcaca
      601 gtattctcgt agagattgat gtcggtgact gacagtttcc agtcttggtt attgaataac
      661 atagtaatta ctcctcttga gagttgagac tatcggtcga ccacaggcgt gttcagtcct
      721 gcggcgtctc cccgcatcgg tcatctcaaa aggtttaaaa ttaggtattg agtagacgcg
      781 ctactaacgg tgtgctgacg acagctagca agctagcgac agtttcatag cgaccagttg
      841 ggctattagc tcgtctcggg attggtgctg gcgggtctca tcttccgggt ctagaccaag
      901 ctctctgagg aggacttcag tccggtagcc acggtaacgg agagtcatgc gcccctcagg
      961 tgtgcgcggg tcaatcatca tcttagattc cctccacctt accggattgt gccagcttca
     1021 ggaggtgctc ttgcttctgc tggtcggtgc cagtgaatgc aataaggatg cctcgcatct
     1081 tgtcaaccat ctcaggctta ccgaagacct ccagcccctt agcgttgaac cggaagttat
     1141 acccacctgc tgagaacttc tggtctgacg gcttaatgat acgaaagcag tcggaatact
     1201 tcggatttac agaccactcg ttactgattg ggttcttacg aaagaggcca cgatgctgga
     1261 gaagtgcggt cattagcttc tgagcgggca gaccataggt tttgcagtag tcgccccaag
     1321 tcacgcctcg gcggttagcg gactgaatgg ctgctcgctt cttctggtct tccagttgaa
     1381 ggactcgacg ctctaacgtc tgaatatggt cgtaacactt ggcgcggagg tgaacggaat
     1441 agccagcgac cagaatgaaa gtatgcttac ggtcgagacg atacagcggc tgctgtttcc
     1501 cctgcgcgtt tagatagaca tcttcagtca ccccgatatg actgctcagt tttgagcact
     1561 gctcagtttt gagcgcgtta agctcctcag ccatattgcg aatatcacgc aggacatttc
     1621 ggtgctcctt gcctgtcagc tcagcaattt caactgaggt catggagaca agctggtcga
     1681 ggttaatgac gctgatttct tgttgggaca tttacgtgta ctccttttgt ttgcaccctg
     1741 aagtcgcacc ctactgacct actcaagccc ccacgtaaag ggagaccagc aggcaacagg
     1801 acgcaaacag aatgacttga ggtaattgtg atgtggtctg cgatgaccag tagtgataaa
     1861 ggtgcaagac agcatgtccg acgtccagat ggtgagcggc gatagaccca agtcaaaatg
     1921 aagacacgag aacgctaacg ggctgggagt ctgacagagg ctgtcttgct agccttggta
     1981 tggagaaaga cttaatgtcg gggggcttcc gcataacttg acgcatcaag accagaggcg
     2041 gaatgtatgg cccctatagt gatagctaag ggtccattcc ctatagtgag ggtataagat
     2101 ttgctacctg ataaggccat aatctgacct ccagttagac ccgaagtatg gagaccacat
     2161 catagcgacc atagtttact tcgggatgac aggaggctgg acttaaagtt cttcttattg
     2221 gaattgtgat aaacacaatc cataacaaga taaactcatg gtctcctcca gcctgctggt
     2281 caggactcaa ggttcaacct cagacaccac tggtctaacc tccttgagtt tccgcctgtc
     2341 cacaatggat ggcagacctc agcaatgatg tcttggttct tcacaatctc gcacaccgat
     2401 ggtctcatag acggaagttc cgctgaggtc tgatgttctc cgcctgtcaa aggagagaga
     2461 ttgctacgtt aaagggaatg gtctcaggcc aaggtgattt aacactaggc gtctgcggat
     2521 ggtttccaat cggtccgcta ccggaagcct gagacttaat gaatgtgtgt gggtcgtgaa
     2581 tgttattaag gtggggactt agggtctctc ccctatagtg atagttaagc ccccttccct
     2641 atagtgaggg tatattcaat ctgacctaaa gctttactcg ctagctgcct gctgctgttc
     2701 cctcgccttc ctgtcccggt cctcaatgcc cttgagcgcc ctttccggca tgctgttaaa
     2761 catggcttcg acttctttcg gtctggtaat ctgctcagtg acaataatgt tcagcagctt
     2821 aaaaagacca tgcgctaact gagggtcgtc atcaatatta atttcacccg gatggaccgc
     2881 ctgattgcca acgatacgac aaatatcagc agctcgctgg accaatggag acaatccttt
     2941 ggctacaagc gacttaatgt ccgtgtcgat atgcttcccc ggctcaccga gatggaccat
     3001 cagcttctga agacttaagc gcaggagtgc ggcagcgcct ttaggagaaa ggttaacaat
     3061 gcttctggcc tccatgtagt cggacttaca gtcgtctggc atgtctgggt taggcatctc
     3121 aacgtttgct gacgctggta taatcatggt ttcgtcatac caataagctt tctcattgca
     3181 tcgttcacat ttagaaaagg cgaaaggtgt ctcataaatc ccgttagcgc catattttaa
     3241 gcggacccag cgctgttgag aataagcgcc acaattcggg cagttaaaag cagtgccacc
     3301 aaactctgcc ggataatact cagccattta ctccagtctc cttataaaca cccgccatca
     3361 cattaaccaa tctatctacc tcgacctctt tcgcgtaacg gtcgtaagtt atagacccag
     3421 agctatgacc cataatcgcc tgagcatacg ccaattcgac cccatgttgt tttagctggg
     3481 tcgcaactgt atgtctaaac gagtgaaaag ctaagccgct acggtcagca aggactttag
     3541 gtagcaacgt tcggttaaac catttgctca cctgctcacc gtagccgttc ttcatcagct
     3601 tgaggccatc gaataccata gcattatcac cctcaatctt tctacggtct tctacgagtg
     3661 atataaagtc agccaataca aa
//
LOCUS       DAJVQH010000222         3653 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJVQH010000222 DAJVQH010000000
VERSION     DAJVQH010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.224"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(4..300)
                     /locus_tag="OQM87_004084"
     CDS             complement(4..300)
                     /locus_tag="OQM87_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCS1509995.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            1052..1600
                     /locus_tag="OQM87_004085"
     CDS             1052..1600
                     /locus_tag="OQM87_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1509996.1"
                     /translation="MAVIGYCRVSTQDQTVDNQKLQIEKVHKVDKWFIDEAVSGATKA
                     TSRDGFRNMMNYVRCDDTLVVVAIDRLGRNTVDVLSNVEALQTKGVRVVSLREGFDLL
                     TPIGRAMLTMMAGLADLEKDLISERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQG
                     KTRLQIQEELGISRATYYRLAK"
BASE COUNT         1132 a          690 c          815 g         1016 t
ORIGIN      
        1 tactcagaac actttcttaa atggtttcac tgaaacgtgt tcatagactc ctgccgctac
       61 gtacgggtca gcatcggccc aggcctgagc tgcttccagc gattcaaatt cagcaataac
      121 ggttgagcca gtaaatcccg cagcccctgg atcgttactg tctaccgctg gcattggacc
      181 agctgtcaac aaacgacctt catcatgcag taactgtaaa cgtgctaaat gtgccggacg
      241 aacggaaagg cgtttttcga ggctatcagc tttgtcttga gcgtaaataa catacaacac
      301 gggcacaact ccttgttcgg gaaagttgta aagtacgtta tttgaaaggg cgaagatctg
      361 caacggaaag atgatgtctt tgttaaggtc atgcataaag taagggtaat tacgccaaaa
      421 atgacatttt cactgatcct gatcgtcttg ccttattgaa tatgattgct atttgcattt
      481 aaaatcgagg cctggttttt ctactgaaat gattatgact tcaataaccc ttgaatgaag
      541 tctattcggt tttctaggag acctgttaaa aacaggacaa tatcaggtga atgactatgc
      601 aatttaatag tagatttcca agtatctaag cagttttaca caaattctat gcagtatcgg
      661 tatagtctct ttttagcata acttattgaa atatatagag aatctgtgaa gtctcttttc
      721 agggaactat gcagggtgtt agtcgttcct ctctcgactc ctagaaaagt gctatataca
      781 ggactatata acgatatata gatgtttggt cacatggtgt aactatcaag aaacttaacg
      841 atttcctacg tgaaagttag aaagtgatgg ttatagttac cctcctaaaa tcttcatagc
      901 tccttcataa ccagtcttaa aaggacttcc ttttgagaca ctttcaacgt aacggtagaa
      961 tgcaccctca agtagtctca aaaggttgtt tttggattat gagacagcta aacttatgag
     1021 actaactttt gagacttcta aggggaacat tatggcggtg attggttatt gcagggtatc
     1081 aacacaagac cagactgtag acaaccagaa actacagata gagaaggttc acaaggtcga
     1141 taaatggttt attgatgagg ctgtctcagg agctacaaag gccaccagca gagatggttt
     1201 tagaaacatg atgaactatg taaggtgtga cgatacgtta gttgtggtag ctattgaccg
     1261 tttagggcgt aataccgttg atgtgctctc taacgtggag gcactacaga ctaaaggtgt
     1321 gagggttgtt tctctacgtg aaggttttga cttattaaca ccaatcggta gagcaatgct
     1381 tactatgatg gctggtttag ctgaccttga aaaagacctt atcagtgaac gtagaaaggc
     1441 tggtattgaa cgtgctaaag ctgaaggggt acatatgggg aggcctgtaa aagcttcttc
     1501 agaggctgta cagacgctta tttcacaagg taagacgagg ttgcagattc aggaagaatt
     1561 aggaatctct agagcaactt attacagact tgctaaataa gttactgaat agttacatgg
     1621 atgtagctga tatttggctt ttttgcaaag gggatcacat agctggtgta acaggtactt
     1681 agcagctaca gacatctgtc agatattttt gaagattctc agagagggaa tttgaagagg
     1741 ggctactatg attttataaa aattttcaga gtctgaaaag ttactgtaca gattttatac
     1801 aaactaaaaa ggactcccgt aggtgattca gtaatgaatt acttcacgaa gagtcccttt
     1861 acaagtcgat ttttaatcgt ggctacgaca atccacccat accagaccta accgtacccc
     1921 accttaccaa gccataccct accccgccag actataagga agcgtagaga accgatgaag
     1981 gtgtctgcac agttgcatct taaacaacca tcacagtgat gtcaaatagt tcttttaagt
     2041 aggaggggtt cccctatggt cacatatccc ctctctcccg cttagtgtcc cttgatagta
     2101 tctagagagt atgcctgtaa tgttagagag tatgcctgta atgtttctgt atcaggtcag
     2161 ctataagagg tctggatatt accttgtaag attcttggta gtgattatgc agagggcttg
     2221 ataagagtgt ttaacgggag gaagagaacc atgaaaccga aggtaggttc tatcttaaca
     2281 gtgactgtaa gagatacctg aaaaaatact ttaacagaac taccaagaag agagggaacg
     2341 gaagagaggg aacgataaga ccagaggtag tgacctgtta aacaggctgt ttcaaagtct
     2401 cttaaaagtc tgttaagagg gctaggtgaa caatcctgcc attttgtcaa gtgttttgtc
     2461 tttagcaaaa agtgctaaac ttaacaaaaa gcactaaatg ctaaagaggc gattaagaaa
     2521 tatactaaga ggggcgcttc gcttggggtc actatgttct ttctctcccg ttaagaggat
     2581 ttacagggac atttcaaagc cctgataagg taagcccgat gataacagaa acagagggca
     2641 aaaccactta tcaacactgt gaacgaataa cgtaaagggc tggttaagaa agtcggttta
     2701 gggattggta acaataccaa ttagaaggga gcttcccttt ctggggtgtc gctctatggg
     2761 tgaagttaac ctttgaagtt ctctgtgaag aaactgaaca gctattatat tggctaatta
     2821 caaccctttg aaagataagc atttttagaa gttactgtta aggttgcaga gttgtctgtg
     2881 aagagacttg tcagttctct atattgggtg gttacaactc attgaaaaat aagagctttt
     2941 agaggttact gtcagagagg tgaatacttg atagatacag tcttccggtt ctgtcaggct
     3001 attttttaac agatagcaca aaatgctaaa gatgccccta tgccatctcg tacagggggg
     3061 ttttgaaggg agtttaacgg gggtctgtgt ggtcattatc cttaaggtca agcgtccatg
     3121 tgcgaccgaa cagtttaaag acaacaagca ctctgacgag gacgtaacga agtagcagat
     3181 ctgcaataag tccaatacct ccaccagccc atacatcagg tgaccccagc tcaggaggga
     3241 gatttaccat tagttttcct tgtaaggcgc tctcaatcgc attctgacac aaaaaagtac
     3301 ctgaccttat gagttgacct gaagaggtca tttaactcgt tacagagcga ttgaagaggt
     3361 ctgtgtgcaa tgcaataacc atgtgaccag atacatagcc acgcctcata ttttactctc
     3421 cccataaacc cattacaccg cagatgttac gaaattgtta aaaatacttg gtattagtct
     3481 ttgaaaatgc tagcattgta tatataagtt aacagaataa taacatgtta tgaggggcag
     3541 aatatgaatg agagacgcag aatttagagc gatgcttcag gcatcaagag aacgcaacaa
     3601 gcacaactcc tacgcttaca caaacaaccc caccagctac gaagtcccaa cct
//
LOCUS       DAJVQH010000223         3518 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJVQH010000223 DAJVQH010000000
VERSION     DAJVQH010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.225"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            190..447
                     /locus_tag="OQM87_004086"
                     /pseudo
     CDS             190..447
                     /locus_tag="OQM87_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OQM87_004087"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OQM87_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCS1509997.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHKQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OQM87_004088"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OQM87_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCS1509998.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OQM87_004089"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OQM87_004089"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCS1509999.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="OQM87_004090"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="OQM87_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
BASE COUNT          907 a          839 c          839 g          933 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgcttgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttagcgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgg
//
LOCUS       DAJVQH010000224         3517 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJVQH010000224 DAJVQH010000000
VERSION     DAJVQH010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.226"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(63..1505)
                     /gene="cusS"
                     /locus_tag="OQM87_004091"
     CDS             complement(63..1505)
                     /gene="cusS"
                     /locus_tag="OQM87_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCS1510000.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPD"
     gene            complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="OQM87_004092"
     CDS             complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="OQM87_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCS1510001.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2335..>3517
                     /gene="cusC"
                     /locus_tag="OQM87_004093"
     CDS             2335..>3517
                     /gene="cusC"
                     /locus_tag="OQM87_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCS1510002.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKMEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQ"
BASE COUNT          862 a          911 c          921 g          823 t
ORIGIN      
        1 ggtatgacta ttgcggcacg ttatttttac actggttata aaagttgccg tttgctgaat
       61 aattaatcgg gtaatgtgat aacaaacctt gtcccccgcg catctgacgt taccgcaacc
      121 gtgcctttat gcgcgacaac aatcgatttc actatcgcca gcccaatacc gctaccttca
      181 ccttttcgct ggcgggaggg gtcaacgcga tagaaacggt caaacaatcg cggtaagtgc
      241 tcgggcgcaa tgggcgtacc ggggttttcg acggaaactt gcacctggtg atcgaccgtc
      301 tgacagcgca ctacaattgt ctctctggtt ggcgtataac gcagggcatt agaaagcagg
      361 ttgcttagcg cccgacgcag catcaacgga tcgcccgcaa cctgacgttt gtcgccaaca
      421 aaccgcaact ccacgccgcg atcttccgct agcgcctcga aaaaatcgaa cactttgccg
      481 acttcatccg ccaggttgag cattttcttt tcggggatta gctggttgtt atcggcctga
      541 gcgagaaaca gcatatcgct gaccattttc gccattcgcg tcagctcttc gagattagag
      601 tagagcacat cttccagctc cttctggctg cgcgactggc tgagggcgat ttccgtttgc
      661 gtaatgagat tagtaattgg tgtgcgaatt tcgtgggcga tatccgctga gaaattggac
      721 tggcgagtaa agacatcctc aatacgctcg atcatatggt tgaacgacag taccagctgt
      781 tccagctcaa tcggcacggt ctgcgggtca aggcgaacgt cgagatcttt cgaggtgata
      841 ttctggattt gacggctgac gctgcggatc ggcgcgtgac ctttatgtac cgccaacagc
      901 acgataaaga cgatcaggat gctgattacc gatgcggtca taataagttt attcatcaaa
      961 tcatttatgt aatgaagatg aaaatcgatc gacagcgcga tgtagagcgt ataaatcggt
     1021 ttgccgtcca ccaacgggcc aaccggcaag ttaatcatcc gccagttgct gtgttccata
     1081 tgcccgtgac cgtggcctgg catcataatc gtcgggccgg aaaggagata cacctcgcca
     1141 ccccgcgcgt ctttatcggg tatggcatca cgtgcaaact cgcggatatc aggcgcaccg
     1201 ggggagtgat acaccgtttt gccgtggcta tctgccaggg aaatcaacac gttggaataa
     1261 ccactgacga tatcttccag cgtcattaag cgtcgggctt gcgtttcgtc agggtgattt
     1321 agtacccgtt caagggtggc gctaatctct tttaaatcat taatgtcctg ctcggcaaaa
     1381 tgcactttta ctgagtggat catgatccat gcaaaggcga aaaacgccgc gatggtggcc
     1441 aggctgataa aaaaggtcag gcgggttgcc agcgaaaacg ggcgctgaaa tggcttactg
     1501 accatccggc acctcaagca tgtaacccac gccgcgcacg gtttgaatca gcttcggctc
     1561 aaagtcgttg tcgattttgc cgcgtagccg cttcaccgcc acatcaatgg cgttagtgtc
     1621 gctgtcgaaa ttcatgtccc atacctgcga ggcgataagc gagcggggca gcacttcgcc
     1681 ctgatggcgg agaaaaaact ccagcagagt aaattcttta ctggtcagag tgatgcgcgt
     1741 gccgctgcgg gtgactttgc ggctgacgag atcgaccatc agatcggcaa cctgaaactg
     1801 actttcgata atcaccgccg ccccgcgccg caggagagtg cgcacccgcg ccagcagttc
     1861 agcaaaagcg aacggtttca ccaggtagtc atctgccccc aactccagcc ccttgacgcg
     1921 atgttcaatg gtgccaagcg cggtaagcaa cagaatcggc atccctttat tggcggagcg
     1981 caacatgcgc acgatatccc agccgttcac gtctggcagc ataatatcga ggattatcag
     2041 gtcataatca ccggtcatcg ccagatggta gccattcagc ccgttgtcgg ccaaatcgac
     2101 cacaaaaccg gcttcggtta accctttggt caagtattct ccggttttct tttcatcttc
     2161 gacaatcaac agtttcatat ttcctccgca tgttgcccgg gcaattctag agtagcggga
     2221 tcagatggca atcgcttatt ggcaaaatga caattttgtc attttcctgt caccggaaaa
     2281 tcagagcctg gcgagtaaag ttggcgacat aaaatcacca gaaattatga gcctatgtct
     2341 ccttgtaaac ttctgccatt ttgtgtggcc cttgcgctaa ctggttgttc actggcaccg
     2401 gattatcagc gtccggtaat gcccgtaccg cagcagttct cactcagcca gaacggcctg
     2461 gttaacgcag cggataacta ccagaacgcg ggctggcgca ccttttttgt tgataatcag
     2521 gtgaagacgc tgattagcga ggcgctggtg aataaccggg atttgcgcat ggcgacgctg
     2581 aaagtgcagg aggcacgggc gcaatatcgt ctgaccgatg ccgaccgcta cccacagctc
     2641 aatggcgagg gcagcggcag ctggagcggc aatcttaaag gcgattcagc cacgacgcgg
     2701 gagttctcga ctggccttaa cgccagcttt gatctcgatt ttttcggtcg cttaaagaac
     2761 atgagcgaag ccgagcgcca aaattattta gccactgagg aagcccagcg cgcggtgcat
     2821 attttgctgg tttctaatgt cgcgcaaagc tatttcaatc agcagctggc gtatgcgcaa
     2881 ttgcaaatag ccgaagaaac gctgcgtaat tatcagcagt catatgcgtt tgtcgaaaaa
     2941 caactgttga ccggtagcag caatgttctg gcgctggaac aggctcgcgg ggtgatagaa
     3001 agtacccgca gtgacatcgc taaacgtcag ggggaactgg cgcaggcgaa taatgcattg
     3061 caactgttat tgggaagcta cggcaagcta ccgcaagcgc agacagtaaa cagcggcagc
     3121 ctgcaaagcg ttaaattacc ggcgggcttg tcgtcgcaaa tcttattgca gcgcccggat
     3181 attatggaag ctgaacacgc gttaatggcg gctaatgcta atattggtgc tgcgcgtgcg
     3241 gcatttttcc cgtctatcag cctgaccagc ggaatatcaa ccgccagcag cgatctgtcg
     3301 tcattattta acgctagcag cgggatgtgg aattttattc ctaaaatgga gatccccatt
     3361 tttaatgccg gacgcaacca ggccaatctg gatatcgccg aaattcgcca gcagcagtcg
     3421 gtggtgaatt atgaacagaa aatccagaac gcttttaaag aggtggcaga tgcgctggca
     3481 ttacgtcaaa gcctggacga tcaaatcagc gcccagc
//
LOCUS       DAJVQH010000225         3514 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJVQH010000225 DAJVQH010000000
VERSION     DAJVQH010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.227"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            289..807
                     /locus_tag="OQM87_004094"
     CDS             289..807
                     /locus_tag="OQM87_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCS1510003.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     SFQGKEWVHLKE"
     gene            814..1047
                     /locus_tag="OQM87_004095"
     CDS             814..1047
                     /locus_tag="OQM87_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCS1510004.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
     gene            complement(1349..1816)
                     /locus_tag="OQM87_004096"
     CDS             complement(1349..1816)
                     /locus_tag="OQM87_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510005.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQQLQIQEVCNSTLWETAKQQLLQESDGRYFAINSKHL
                     DFPGSGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFSHNLT"
     gene            complement(2886..3077)
                     /locus_tag="OQM87_004097"
     CDS             complement(2886..3077)
                     /locus_tag="OQM87_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1510006.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            3142..3447
                     /locus_tag="OQM87_004098"
                     /pseudo
     CDS             3142..3447
                     /locus_tag="OQM87_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
BASE COUNT          844 a          787 c         1042 g          841 t
ORIGIN      
        1 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
       61 gcagggcgct gacactccct gataatctgc cggaaacgaa acaggcgctt gcagagagcg
      121 tcacacgcgg gatagacgct ctttatcatg tcggggagat aaaaggccgt caggatgtga
      181 ttcaggcgct cacagaggca gggcttgaag tggtcagggt gacgcgaagc agtatcagca
      241 ttgcagaccc gaacggtggg aagaatatca ggctgaaagg agcattctat gagcaatctt
      301 ttacagacgg gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa
      361 atgctgaacg acgagttcag gaagctcgaa gaatctgtaa gcaaggctgt gacatcaaac
      421 gagacgaaaa tcaaagacgc tatagccctg ttcacagctt cgacagagga atcactgaaa
      481 aaacaccggg aaggggtgaa agaggcgatg atgcagcaca ggaaggacgt gttaaagctg
      541 gcagggaata cgggcatgat gttactgggg atggtcattt tcctgtttac cgtgagtggc
      601 gggacgctct ggtatcttgg cgggatgata caggcgaacc tggaagaaat caggaagcag
      661 aacgagagtc tggagaagct gaacgcgaag acatggggcg tggagtttgt gcaggacggg
      721 aacaggaaat ttctggtcct tccttacggg aagtcagcag aggtgatttc ctttcagggg
      781 aaggaatggg tacatctgaa ggagtgacac agagtgacag agctggaaac acatttgctg
      841 aacgccttag agcagctgca acaggactat atgcagcggc tgagcgaatg ggagagcgcc
      901 ttcgtggaat tgcagaaaat gttttcgctt acgcaacggg acaacgcgat gctgaacgag
      961 cgggtcatgc agttgagtca gcaggtgcag cacttgagcg agcagacaga acgcttgagc
     1021 cagttataca gcgagaactg gagataagag aggaacggct gatacaggag cgcgaacatg
     1081 cgttatccct ggaacgggag cgtcagccgg aaatacagga acgcacgctg gatggtcctt
     1141 cgctgggatg gtgaaaccat gaaaaatggc agcttcagtg gattaggagg ggttaatgtg
     1201 gcctgtacca tctggttgca taggtattca tgcggttaaa atttatcagg cgcgatcgcg
     1261 gcagtttttc gggtggtttg ttgccatttt tatctgtctg ctgccgtgat cgcaccaaac
     1321 gcgtttcagt ggtgcgggca ataagatatt acgttaaatt gtgagagaat ggtccccata
     1381 tactgacttt atcatacgga tagggttggt agtgggtcga ttgggtttcg gcattgtttc
     1441 cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat tctcctgagc
     1501 ctggaaagtc taagtgttta gaattgatgg caaaatatct tccgtccgat tcttgaagta
     1561 gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt tgtagttgtt
     1621 gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt attattctct
     1681 cttttaataa gctgtttacc acaggattta gttgttgtct aaagcacgca gataatgcca
     1741 ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt gtttgatgtg
     1801 atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg gctataaaac
     1861 aaaattttca tagagtgatt caagatcttc ttgagatctt ttttggcggt gcgtaatctg
     1921 ctgcctgcaa acaaaaaaac caccgttatc aacggtggtt tgtttgccgg aatctagagc
     1981 taccaactct ttcttccgaa ggtaactggc ttggtggagc acagatacca aatgctgtcc
     2041 ttctagtgta gccgcagtta ggccaccact tcaagaactc ttaatatctc aatccacctt
     2101 atccagttac caatggatgc tgccagtggc gttaagtcgt gtcttaccgg gttggactca
     2161 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2221 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa
     2281 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2341 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2401 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2461 ctatggaaaa acgccagcgg cgcagccttt ctttgccctt tgcctttttg ctcacatgtt
     2521 ctttccggcc ttatcccctg attctgtgga taaccgtatt accgtctttg agtgagctga
     2581 taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga
     2641 gcgccggaag ctgcattttc tccttacgca tctgtgcggc atttcacatc ggctatggtg
     2701 cacttttcat accatccgca ctgatgccgc ataagtaagc ctgtgtacac tccgctatcg
     2761 ctacgtgact gggtcatggc tgcgccccga cacccgctaa aacccgctga cgcgccctga
     2821 cgggcttgtc tgctcctgac atccgctcac aggcaagctg tgaccgtctc cgggagctgc
     2881 ttgtgtcaga ggttttcacc gtcatccccg aagcgtgcga ggcagctgcg gtaaagctcg
     2941 tcggcgtggt cgtggagcga ttcacagata tcagcctgtt catccgcgtc cagttcgttg
     3001 agcttctcca gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     3061 ttttcctgtt tagtcactga tgcctccgtg taagggggat ttctgtttta tggggtaatg
     3121 ataccgatga atcgcgagag gatgctcacc atacgggtta ctgatgatga acatgcccgg
     3181 ttactggaac gctgtgaggg taaacagctg gcggtatgga tgcggcgggt ctgcctgggt
     3241 gagccggttg cacgctccgg gaagctgccg acactggcac cgccgttact gcgtcagctg
     3301 gccgccattg ggaataacct gaatcagaca gcgaggaagg tgaacagcgg gcagtggtct
     3361 tccggtaacc gggttcaggt ggtggccgca ctgatggcca tcggggacga gctgcgccgg
     3421 ctgcgtctgg ccgtcaggga acaggggcgc gggatgatag ttaagtttca tgcccgggga
     3481 aaaggtggtg gcagtggtcc ggttgattac ctgc
//
LOCUS       DAJVQH010000226         3381 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJVQH010000226 DAJVQH010000000
VERSION     DAJVQH010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.228"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..935)
                     /locus_tag="OQM87_004099"
     CDS             complement(<1..935)
                     /locus_tag="OQM87_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HCS1510007.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLAKNSRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFIVYSFACFFIGKFIANILMT"
     gene            complement(963..1883)
                     /gene="rbsK"
                     /locus_tag="OQM87_004100"
     CDS             complement(963..1883)
                     /gene="rbsK"
                     /locus_tag="OQM87_004100"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS1510008.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGMQSSYPSIEQFNEFLTLNE"
     gene            2189..2971
                     /locus_tag="OQM87_004101"
     CDS             2189..2971
                     /locus_tag="OQM87_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCS1510009.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPALSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(2973..3199)
                     /locus_tag="OQM87_004102"
                     /pseudo
     CDS             complement(2973..3199)
                     /locus_tag="OQM87_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT         1002 a          772 c          672 g          935 t
ORIGIN      
        1 gtcataagga tgttggctat aaacttgcca ataaagaagc aggcaaagct gtagactatg
       61 aagtttgaag catcacgttc gttgatatcg cccaactcca gtgccagacg gatggtaaat
      121 gaccatactg cgacctgcat acccacataa aggaattgag ccacaatacc acgacgaaag
      181 cgcgaatttt tagccagata gcgcagcgta tccattgctg acgggcgttt atggtgactt
      241 gtctgtgcca ctttacaggt tgggaagcgg gttaaaagga acaacaccat gaccacaacc
      301 agaatcataa tcatatactt atacggttca agggtgttct ctaacatcag caccttaaag
      361 ttgtgaattt gctcggcgtt cattccggac atctgcttct caaggctttc cccctcggag
      421 aaaaccagat atttgcccaa taaaatacca gacgcagcac caatcggata aaaggtctgg
      481 ctgatattga gccgcaatgt ggcataggct tttggaccga tcattgaact gtatgtgttc
      541 gctgcagttt caaggaaact caggccaatc gcaatcgcaa aaatagctgc aaggaacata
      601 gtgtaggttg ccatatgcga ggcagggaaa aaaagtgtac aaccaccaat atacagcgtc
      661 aggccaatta aaattgccac cttataactg gtctttttaa tcacaaggga tgctggtatt
      721 gcaattaaaa aataacctcc ataaaatgcg ctctgcacca atgctgaagc aaagttactt
      781 agcgaaaata cacttttgaa ttgagtgatt aatatgtcat ttaatgcagc tgcgcatccc
      841 catagcggga ataaacacga taacaaaata aactggaaca agggagtctt attcagatac
      901 ccatcaggca tctgaatgat gtttttatcg ttcatagtgc tacctttaac tgtgcaggat
      961 gattattcgt ttaaggttaa aaattcatta aattgttcaa tactcggata agatgattgc
     1021 atacctttcc ctgtgacgct gaaagcggca aagagagcgg cttttttcaa agcggcttca
     1081 acatcaccgc tttgaacata ataatgggaa aagcaaccaa taaatgcgtc accagcgcca
     1141 ctagtatcaa cagcatttac tttgaatgca ggaacatgga cttcctgatc gcgggtcatc
     1201 cataatgcgc ctttttcgct catggtaaca ataatattgt tcagcccttt atcaactaac
     1261 gaacgtgcgg ccaaacgaat atgatcataa gtatcaaccg acataccagt taatatttcc
     1321 agttctgttt cattcgggat aaagaaatca cctttgcagg cataagacat atctaactca
     1381 cgcaatgccg gagccggatt taataacact tcaataccat ttttcttacc aaactcaatc
     1441 gcgtggtaaa ctgtttccag ttgaacttcc agttgtaaaa cgatcaattt gcattttttc
     1501 agatcttctg cagctcgatc gatatcttcc ggggaaagaa atttattcgc tcccttaatt
     1561 attaatatac tattgctcga gttggcatta acaaagatcg gtgcaacacc actgctggta
     1621 caggggacct tctcaacata agtggtatta attccccatg attcaagatt acgaatagta
     1681 ttatccgcaa aaatatcatc acctacttta gtcagcatca ggacttttga attcaattta
     1741 gccgccgcca ccgcttgatt agcacctttc ccaccacatc cgattttgaa ggcaggtgct
     1801 tccagagttt ctccttcttt aggcatctga ttagtgtaag taatgagatc caccatattg
     1861 gaaccaataa ctgcaatgtc catttcacta cctcttataa actttcgcat aacaatggta
     1921 tttaaataac attagcatgt tacttttgca tcatttgtga ctgagatcgc gattagcaca
     1981 tcaacccgat gtttatttaa tagacttcca gtctcatcac tcaggccaac actatctaat
     2041 cataagcaac ctaacaagat tagtgcccaa aattcagcag cctataccct tttcatttca
     2101 aaggggcggt cgtatagtat ggtaatgaaa acaatgttta ctaacgccaa aatgttattt
     2161 ttataacatt cttacggaga gagagttgat ggaaacgaag caaaaagagc gtatccgacg
     2221 tttgatggaa ctgcttaaga aaaccgacag aatccatttg aaagacgcag cgcgaatgct
     2281 ggaagtttct gtaatgacta ttcgtcgcga tctccatcag gaagatgaac ctctgccact
     2341 gaccctactg ggtggctata ttgtaatggt gaataaaccc gcgctatcca tgccagtaat
     2401 ccatgacgtt ccaaaaaatc atcgtgatga cttacctatt gcaattctgg ctgccggaat
     2461 ggttaatgaa aatgatctga tcttctttga taatggccag gagataccac tcgttataag
     2521 catgatcccg gatgcaatca ccttcaccgg catctgttac tcacatcgcg tctttgttgc
     2581 gttgaatgaa aagcctaatg taacagcaat actttgtggt ggtacgtatc gtgccagaag
     2641 tgatgctttt tacgatgcca gtaactcttc gccattagac tctctcaatc cgcgaaaaat
     2701 atttatttcc gccagcggtg tgcataatca ctttggcgtc agctggttta accctgaaga
     2761 tcttgccact aagcgtaaag cgatgaaccg tggactacgg aaaattttgc tcgcccgcca
     2821 cgcgttgttc gatgaagtgg cctctgccag cctcgcaccg atctctgcat ttgacgttct
     2881 gattagcgat cgtccgttac cggcagatta tgttacgcac tgccagaatg gttctgtaaa
     2941 gatcattaca cctgattcag aagacgaatg acttactgaa aaaacaccac aatcttgtta
     3001 aacatcgtcg ggttggactg attacgttgc actttcacca catattccag cttatctatt
     3061 tggcttatca cctactccag acgctggtca tccttgacca gtagccagat atggcttttg
     3121 tcgcagtcct gaatcggcag gcagagaatg tcttcaacgt taagagcgcg acgggcaaaa
     3181 gcccacaaag gtgagtcatt acacccggat ggtaaacagg cacttccttg ttgtagaagc
     3241 cgttatattg tccataatgt ctgggttttt aatgcatact gggtaacaac cggacgaatc
     3301 ttcgaatatc actggcgctg ccagccggtt tgaattcatc tcacaaccct gcatagagcg
     3361 aatctcttgc ctgcacgtca c
//
LOCUS       DAJVQH010000227         3337 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJVQH010000227 DAJVQH010000000
VERSION     DAJVQH010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.229"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            362..666
                     /locus_tag="OQM87_004103"
                     /pseudo
     CDS             362..666
                     /locus_tag="OQM87_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            835..2199
                     /locus_tag="OQM87_004104"
     CDS             835..2199
                     /locus_tag="OQM87_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1510010.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALEHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGFSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
     gene            2249..2458
                     /locus_tag="OQM87_004105"
                     /pseudo
     CDS             2249..2458
                     /locus_tag="OQM87_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(2552..2743)
                     /locus_tag="OQM87_004106"
                     /pseudo
     CDS             complement(2552..2743)
                     /locus_tag="OQM87_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2784..3281)
                     /locus_tag="OQM87_004107"
                     /pseudo
     CDS             complement(2784..3281)
                     /locus_tag="OQM87_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          825 a          740 c          784 g          988 t
ORIGIN      
        1 cgtataaaca tctttgacgc cagcgagata cagccatcct tcccgggtgg caacatacgt
       61 tatatctgcg acccagacct gattaggggg gttggggagc aatggaacag taaacgccgt
      121 taagaagtca atttaaatag ccaaaaatta actagccaat ttacattgca ggttgtaatt
      181 tttaaccacc ttttttacga tagccaaatt acctataccc atcccggccc tgccaagcca
      241 tacccgctat cggcgatgaa aatgcttttg tgcgcggtcg gagtggtccc gacgagggtt
      301 ttacccgaag tcggggcgta tctccgcgtt agcgggccgt gagggccgct tacgagcgtg
      361 tatgagagac gtacagtggt tttgtccgct cacaccagta catacaggta cggaaataaa
      421 accagtcgaa atgccggtaa cgacgattac tcctgtctct gatgccggtg gactacaaga
      481 ttttatttac tggcgtcctg atgcggacag gactggtatg gaacctgttt atgtgatact
      541 taatgatcct ctggattccg ggcgatttac gcgtaaacaa ctaaataaaa atatcttaaa
      601 catgcaagta attttggtat tctcgataca agaataaata gtagtaacta ctatagttgc
      661 aaataatgtt aaaaacattt ctatgatcat ctttacattt tttatgattg acaattgttt
      721 ttggttaaaa aaagacaact aatgagtgtg ataatgcaat actttgagta attatataat
      781 gataagtaaa caggtttgcg acaattggtt atcagtatta cagaaggatt aaatatgtca
      841 ttattcacag aacctaagga aattgaaaga ttaccctctg aagagattga acgattatat
      901 cccgtcctgc gatacagggt ctttatcagc attttcctcg gatatatggg gtattatttt
      961 gtccgaaaca ccaccagtgt gttatccggt gtgctgcaca tgtctgcaac cgagatcggc
     1021 attatctcct gtgccggttt tttatcctat ggtatatcaa agtttgtttc cggacttatt
     1081 tctgacagaa gtaattcaaa agtatttttg tctcttggtt tattcctgtc cggactggta
     1141 aattttctga taggatatat tcccgggatc atcacatccg ttacgttatt ttcaacaatg
     1201 tatttactta acggctggat ccaaggtatg ggatatcctc ctggtgccaa aacactggtg
     1261 ttctggtatg aacatcggga aaggatcact tgggccacac tgtggaattt atcgcacaat
     1321 gtcgggggag ctctggcccc cgttctgatt ggtttcagtt ttggtttttt cggcgacagt
     1381 gctctggagc atgcccgggc cgcatttatc ttcccaggag tactgtgtat ggcaatgagt
     1441 gtgctgattt actttattca ggtggacaga cctgtatcag tggggcttcc tcccattgaa
     1501 gaatggaaag gtaacgtcgt ttcacacccg gctaaaggcc gtgaacaagg tccgagactc
     1561 tccattcctg atatcatcag aaaacatatc atccgaaata ataaactcat ttactgctgt
     1621 atctatggtt cgtttgttta tattctgaga tacggtattg tttcctgggc tcctaagttt
     1681 ttaagtgaca gtctggatgt cggtggtaag gatatgggaa aactggcatc aatggggggg
     1741 ttttctgttt ttgaaatcgg tggtgtggct ggtatgcttc ttgccggata cctgtctgtc
     1801 agactgttca gaaactcaaa accattaaca aatactttat ttctggcact taccattatt
     1861 ttattaatcg cgtactggta cgtgccatca ggcaatgaat atctctggct aaactataca
     1921 atccttattc tgttggggct ggcagtgtat ggtccggtga tgtttattgg tctttattca
     1981 atggagcttg ttcctaaaga agctgccgga gccgcatcag ggctgtcagg cacattttcc
     2041 tatatcttcg gctcaattgt tgcgacactg ggaatgggac ttgttgtcga ttatctggga
     2101 tggggggcca catttatcgt actgatactg tctgctgttt ttgccatcat tttcacactg
     2161 atgagtcgtg aacgctcact ggagttcgaa aaagagtaat tttctcagga aaaacataaa
     2221 attactgact aaatatttat tttttgaatc gcagggcatt ccggataagg ttgatatcgt
     2281 gtcacggctg aagacctatg gatacagtat ttctggtgtg gagacggatg acggatataa
     2341 ggcgcttgtc cgcgcttttc agcttcattt ccggcagaaa aattatgacg gcataatgga
     2401 tgctgaaacc gctgccatcc tgtatgcatt gctggagaag tatttcccgg gtaaataagg
     2461 agtgagtcgt cagagctgcg atatatctcc ttcctccatg ggctgtctct tatacacaaa
     2521 tccccacccg gggatttgtg atgtcagatg tccccagtaa gactatgttt tcattgcgct
     2581 ctatgaagct gagtgagcgt aacgactgga gctgcttctg cggtgctccg gtggcgaacg
     2641 tgaagtcata ctcttcgaac attttcaccg ccgggaaggc tgccattcgg gtatacatcg
     2701 cctgtttacg ttgatgacgt gccagttttt cttcatgaag catgatgctc caagtaaacg
     2761 ttcaccgaac accgtattga cgattatcct gatggtagca ccaccttcca tggcaggagg
     2821 tcgtcaactc ggttggaagg ccattccggc agtacgctca gaatatggcg cagatacgct
     2881 tccggatcga taccgttcag acggcaggtg ccgatcagcc cgtacagcag tgcaccacgc
     2941 tcgccgccgt gatcgctacc gaagaacacg taatttttct ttccgagaca gactgcacga
     3001 agcgctcttt ccgctgtgtt attgtccgcc tccgccagac cgtcatcact gtaataacag
     3061 agggcgtccc actgattcag tacatagctg aatgcttcgc ccagtctgga ttttttcgac
     3121 agcgtgccat tcttctccac catccattca tgcagcgacg tcagtaacgc tttgcttcgc
     3181 tgctgcctga ctgcaagaca ctctgactct ggtaatcccc gtatttcatc ctcgatggcg
     3241 tacagttcac tgattcgctt cagggcttct tctgccgtcg cacgcaagta atccggggcc
     3301 cgctatgccg cgctctatcg ggcgggacgg ggctggc
//
LOCUS       DAJVQH010000228         3265 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJVQH010000228 DAJVQH010000000
VERSION     DAJVQH010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.231"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            41..457
                     /gene="torD"
                     /locus_tag="OQM87_004108"
                     /pseudo
     CDS             41..457
                     /gene="torD"
                     /locus_tag="OQM87_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
     gene            complement(550..855)
                     /gene="cbpM"
                     /locus_tag="OQM87_004109"
     CDS             complement(550..855)
                     /gene="cbpM"
                     /locus_tag="OQM87_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCS1510011.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(855..1775)
                     /gene="cbpA"
                     /locus_tag="OQM87_004110"
     CDS             complement(855..1775)
                     /gene="cbpA"
                     /locus_tag="OQM87_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCS1510012.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFFEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            2036..>3265
                     /locus_tag="OQM87_004111"
     CDS             2036..>3265
                     /locus_tag="OQM87_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCS1510013.1"
                     /translation="MGSNIHGMSCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPRISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQL"
BASE COUNT          904 a          687 c          759 g          915 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcattacct gccacgctga cagtacgtga
       61 cgatgcccgt ctggaactgg ccgctgactt ttgcggcctg tttctgatga ccgacaaaca
      121 agcggcgctg ccgtatgcat cggcctacaa acaggacgag caagagatta aacgcttgtt
      181 agttgaggca gggatggaaa ccagtggcaa tttcaacgaa ccggcagatc atctggcgat
      241 ctatctcgag ttgttgagtt atctgcattt ttcgctggga gaggggaccg ttcctgcgcg
      301 aagaatcgac agtttgcggc aaaaaatact gacggcgcta cggcaatggt taccagagtt
      361 tgctgcgcgt tgtcgacagt atgacagctt tggtttttac gcggcactaa gccagttatt
      421 gctggtgtta gtggagtgcg accaccaaaa cagataacgt cgtttatgcg cctgaaaaga
      481 cgcgtttagc gtcgcatcag gcattatggc gcagttgccg gatgcgtcat cacatccggc
      541 aaacacacat cacggatgag ctacaaaccg ggaaagccgc tggcgtagca ggcggttttc
      601 ctgcttcagg tgcgcaatat catccattaa cgtcagcgcc accgcgatcc ccggccagtc
      661 cagagccagt tcatgacgca ggcgtaccgc gcgttgcacc acaatggcgg catggtcgtc
      721 aaatacccag gttgtttcct ggatctcacg cggttcaacc acccccaaac cgacaatttc
      781 attcaactcc tcttcagaga tgccggtatg caggcaaaat tcggtaatag taaaagtcac
      841 cgtaacatta gccattatgc tttcccccaa tctttacgtg gatcaaaaga cgactgggcg
      901 tctgccagtt gctgccacag cgcggcagtg ttttcatccg gtttcggcgg catcacgatt
      961 ttcagtaccg catacagatc gccggtctgt tttttgctca ccagaccttt gcctttaacg
     1021 cgcaatcgtt gcccggcctg gctgcctggc gggatagtca gcaaaatgct ttctttcagt
     1081 gttggaacgg tgactttagt acccagcgcc gcttcccacg ggctaaccgg caccacaatt
     1141 tccagatcct ggccgacaat atcgaacagc ggatgtggcg caatatgaat caccagccac
     1201 aaatcgccat ttggaccgcc gttttcgccc ggcgtcccct ggcctttcag gcggatgcgt
     1261 tgaccattgc cgacgcctgc cgggatcttc acattcagcg ttttcgggat ttcctgttcg
     1321 atcatgccaa aggcgttata aaccggcagg ttatagctga tggtacgctt atgctcagta
     1381 agcgtttctt cgaagaatac cgccacttcg atttcaatat cgtggccgcg tgcggcgggg
     1441 cgttgacggc tctggcgggc atgctgaccg aaaattgacg agaagatatc gtcaaaatct
     1501 tcggcattaa aactctgacc gtcgccatga tggaactgac ggttaaattg cggatcgttg
     1561 cgatgttgcc acatctgatc atactcagcg cgacgttgtt catcacttaa cacttcccag
     1621 gcttcagcga cctctttgaa gcgggcttcg gcatccggtt ctttgctgac atcaggatgg
     1681 tatttacggg ccagtcgacg ataggcggtc ttgattgtct tgagatcgtc cgtcggtttc
     1741 acgcccatga tggcgtaata atcctttaat tccatagcgt tatctcgcgt aaatcaacac
     1801 aaattgaagg aacccctgta aggtaactcc tataagtgta gggtaatcct caaaatttca
     1861 tatgccaaca cagaatatgt tattgaaatc atcgcggaga ggaggtcgcc atcaagatgg
     1921 gttgctgaac atattttaaa caggtgaaaa agggtgagcg atttttgata gttgaaccag
     1981 gcactttaag tttaactagg gcgtcattat ttattaaatt ttatagacgc tatatatggg
     2041 tagtaatata catggaatga gttgcactgc aaataattat ttgaaacagg cctggaacga
     2101 tataaaaaat gagtacgaaa aaaatcaaac atattcaatc acgctttttg aaaacacact
     2161 ggtgtgtttt atgcggttgt acaatgaact cagacgtaaa gtaaatgaag aggatactcc
     2221 atgtctggaa tgtgaatcac tagaaaaaga atttgaggaa atgcagaatg ataatgatct
     2281 atcattattt atgagaacat tgcgtactaa tgatacacaa atttattcag gggtttcagg
     2341 aggtattaca tatactatac aatatgttca agatgttgat attgttagag tgtccttgcc
     2401 gggcagaggt tcagagtcta tcacagattt taaaggttat tattggtatg gctttatgga
     2461 atatatcgaa aacattaatg cgtgtgatga tgttttttct gagtattgtt tagatgatga
     2521 aaatatgagt atccagccag aacagataaa tatgccgaga atatctgatt tagatactgg
     2581 tattgatttg tctggcatat cttttattca gagtgaaatt aataaaacat atggattaaa
     2641 atacgctcca gtagatggcg atggatattg tctgttaaga gccatactgg ttttaaaaga
     2701 acatgagtat tcatgggcgc tgggcagcca taagacgcaa aagcaagtat acgaagagtt
     2761 tattaaaatt gttgataaac aaacgatcga ggctcttgtt gatacggcat tctataatct
     2821 cagggaagat gtaaagacat tatttggcgt tgatctgcaa tctgacaaca aaattcaggg
     2881 tgtgggtagt tttatgtcat ggagctttct gttttttaaa aaacaattca ttgatagttg
     2941 cttgaatgac aaaaaatgca tactgcattt acccgagttt atatttaatg ataacaagaa
     3001 tttgcttgct ttagataccg acacgtcgga taggattaaa gcggtgaaga attttcttgc
     3061 tgttctttca gatagcattt gctcattatt tattgttaat agtaatgtgg catcaatctc
     3121 cttgggaaat gaatcctttt caacagatga agatcttgag tatggttatt taataaacac
     3181 tggcaatcat tatgatgttt acctccctcc tgaacttttt tctcaggctt ataagttaaa
     3241 aaataaggaa atgaatgcgc aactt
//
LOCUS       DAJVQH010000229         3212 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJVQH010000229 DAJVQH010000000
VERSION     DAJVQH010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.232"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..191
                     /locus_tag="OQM87_004112"
     CDS             <1..191
                     /locus_tag="OQM87_004112"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510014.1"
                     /translation="RDLTAVPVAHPAKFWQKNTAVFLIELNALRIAECIILSPLFESG
                     ERRPLLEKVFAGLLRVFQ"
     gene            complement(129..434)
                     /locus_tag="OQM87_004113"
                     /pseudo
     CDS             complement(129..434)
                     /locus_tag="OQM87_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            502..1117
                     /gene="tnpA"
                     /locus_tag="OQM87_004114"
                     /pseudo
     CDS             502..1117
                     /gene="tnpA"
                     /locus_tag="OQM87_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1445..2527
                     /gene="ompC"
                     /locus_tag="OQM87_004115"
     CDS             1445..2527
                     /gene="ompC"
                     /locus_tag="OQM87_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1510015.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKVNL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3016..>3212)
                     /locus_tag="OQM87_004116"
     CDS             complement(3016..>3212)
                     /locus_tag="OQM87_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1510016.1"
                     /translation="EGISFQSILDDVRLNNALSAIQTTVKPISEIARENGYKCPSRFT
                     ERFHNRFKITPRELRKASRE"
BASE COUNT          931 a          709 c          648 g          924 t
ORIGIN      
        1 cccgtgacct gacggctgtt ccggtagcgc atccagccaa gttttggcag aaaaatacgg
       61 ctgttttcct gatcgagctt aacgccctgc ggatagcgga atgcatcatt ctgtcaccgc
      121 tttttgaatc aggggaacgc cgaccgcttc tggaaaaagt ttttgcaggc ctgctcaggg
      181 tctttcagtg actgttgcaa tggctgtgac ggagcatctt taatccattg cgtttcagtg
      241 gcatttttcc actcaaccaa tcaggaagcc attttcgcgt aagggatgtc tttgttaccg
      301 gccttatgat tctcattctg acgtgccaga gtacgactga aaacgaaacg acatgcgcct
      361 gcgaagcgcc tcatttcacg cgcctgttga ccaccgggtc ttaaccggaa tttgaatacc
      421 tgtagtcgtt tcacagggca aattatactt tggtctgtga aaaaaactga tatccgacgc
      481 ggaaggcact gtgttttcct gatgcatgct caccgggtct ttgttaccaa gtaccgacgc
      541 cagatttttg accatgacgc gacagaaaaa ctacgtactt acttttcaaa tgtatgtgct
      601 gattttgaag ctgaactggt tgaaatggat ggcgaaccag agcacgtcca tctgttaatt
      661 aactatcccc ccaaactggc gatatccagt ctggtaaaca aacagcctca aaggcgtatc
      721 gggtaggtta ctgcgacggg atcggccaga tattgctgcc aggtattacc gcaaagatgt
      781 tttgtggcgt cccggctatt ttgccagtag ctgcggaggt gcgccaatat ccgtcatccg
      841 ccaatacatt gaacaacagc aaacacctgg tcaggtgaaa aaacgagcct tatatccccg
      901 ccctgaagga cgggctttta cggtgcactg gataagcctg gagattatct acacctgcat
      961 ggacatcatt gtactatgcc ccgtacatgc cccatcacat cctcaacatc aaagtattag
     1021 agccttgcaa accattatgc gtctcagttt gtctcatcta tcttacgcaa aaaaaataga
     1081 actaatatca agcggcgcta tattgcgtat taaataaaac cacctcaatt acattaataa
     1141 atctaaataa atatcataca aataatttcc atggttattt ttaggctatt taaattaaaa
     1201 aaataaatta tgagtcaatc ataaataaca acctttaata taatttgact accaaattta
     1261 tattcgttca caaaatagtc atgcattgtg tgcaatagaa acagttcaga taaaaatagg
     1321 gatctacttc acaatttaaa tgagaaacta aaacttacat cttgaaataa tctcattgat
     1381 tggatgaata tttgtagtgc agtgacatca tttttaaata atagttcaaa taaaagggct
     1441 cacgatgaaa aaattaacag tggcaatttc tgctgtagct gcatcagtac tgatggcgat
     1501 gtctgctcag gcagcagaga tttacaataa agacagtaac aagctggatc tgtacggaaa
     1561 agttaatgct aagcactact tctcttctaa cgatgcagat gatggcgaca caacttatgt
     1621 tcgtctgggc ttcaaaggcg aaactcaaat caacgatcaa ctgactggtt tcggtcagtg
     1681 ggaatatgaa ttcaaaggca accgtgctga atctcaaggt tcttccaaag ataaaacccg
     1741 tcttgccttc gctggcctga aattcggtga ctacggctcc atcgattatg gccgtaacta
     1801 cggtgtagca tacgatatcg gtgcatggac cgacgttctg ccagaattcg gtggcgatac
     1861 ctggacccaa acagatgtgt tcatgactgg tcgcaccaca ggtgttgcaa cctatcgtaa
     1921 caacgacttc tttggtctgg ttgatggtct gaactttgct gctcagtatc agggtaaaaa
     1981 tgaccgcact gacgtaactg aagctaacgg tgatggtttc ggtttctcca ctacttatga
     2041 gtatgaagga ttcggtgtag gtgcaaccta tgctaaatct gaccgcacta atgatcaggt
     2101 tatctacggt aacaacagcc tgaatgcatc tggtcaaaat gctgaagtat gggcagctgg
     2161 tctgaaatat gatgcgaaca acatctatct ggctaccacc tattctgaaa cccagaacat
     2221 gactgttttt ggtaataacc atattgccaa caaagcacaa aacttcgaag tagttgcaca
     2281 atatcagttc gacttcggtc tgcgtccgtc cgttgcttac ctgcaatcta aaggaaaaga
     2341 cttgggtgcg tggggtgatc aggacctgat tgaatatatt gatgtaggtg caacctatta
     2401 cttcaacaaa aatatgtcca cttttgttga ttacaaagtc aacctgattg ataagagcga
     2461 tttcacgaaa gcatctggcg ttgctaccga tgatatcgtt gctgtaggat tggtttacca
     2521 gttctaatct gattactcaa agatatgttg tgggaggctt tgcctcccca acatataagt
     2581 ggcgccctca agccactttc cttagaagca ctatcttgct tctgactata taaattaccc
     2641 tttattttct gggacgtttc aacgccccat ttttaataat actcagtgaa caattaacgt
     2701 atcaattaga tttaataaca acgatatccg tacctaacct gatatctaat gtcataagca
     2761 tccctttaat tatcccctca acctcttgta aatttcccac tcttgagctc cacatagtcc
     2821 aaacgccgga tgcaaaaaaa atcacacgtg catctctgaa attgtgatgg aggaagtgag
     2881 tatcaattat atgtgcttat aaacgttccc gattggcaca gaagtcaccg gattgttcag
     2941 gctccaatag gttgagtatg tcgtatttat tttccaaaat aaaaatcgat gctgttgctc
     3001 cttaataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg ttatcttaaa
     3061 acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt ccctggcaat
     3121 ctcgctgatg ggttttactg tcgtttgtat agcagacaac gcattattta acctcacgtc
     3181 gtccaatata ctttgaaaac ttattccctc gc
//
LOCUS       DAJVQH010000230         3177 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJVQH010000230 DAJVQH010000000
VERSION     DAJVQH010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.233"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            847..1041
                     /locus_tag="OQM87_004117"
     CDS             847..1041
                     /locus_tag="OQM87_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1510017.1"
                     /translation="MNKQQQTALNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHEL
                     AEELQNSIQIRFEAESEIGT"
     gene            complement(2066..3175)
                     /gene="fepE"
                     /locus_tag="OQM87_004118"
     CDS             complement(2066..3175)
                     /gene="fepE"
                     /locus_tag="OQM87_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCS1510018.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          941 a          782 c          657 g          797 t
ORIGIN      
        1 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
       61 taaaaaccat tcagcagata tttaacataa tatcttattg cgcgcaccgc taaaacgcgc
      121 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
      181 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
      241 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
      301 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
      361 aaaaaaacac accgcgccgc tagcagcccc cccctaatat aagatgatgt ttttttatga
      421 aaatagtcag cccccccccc taaaaagcgg tgttggcgca gctacaacac cgcgccgaca
      481 ccgctttttt aaatatcaca aagagagcaa gaagaactaa tttgtcataa ctctctattt
      541 ataaagaaaa atcagcaaaa acttgttttt gcgtggggtg tgggcatttc aggcatgaga
      601 acagactcgc gtttttccgg agtatcagcg gaattattga ttaaggaaca ctcgacgcag
      661 agcgaaattt tatgcaacag cggctgcact gcacagggat tatttatcag ttgatatgct
      721 gtgccatgat acgtcctaaa gaaacaggac aggatgtgca attgtaatac cgtcgcccgc
      781 aacgtcttac aattaccacc tgctcagccc cccccgacac ccggaactga cggagaacaa
      841 cggaaggtga acaaacaaca acaaactgcg ctgaatatgg cgcgatgtat caaaagccag
      901 agcctgacac tgcttgaaaa actggatgct ctggatgccg acgaacaggc ggccatgtgc
      961 aaacgactgc acgaactcgc agaagaactc cagaacagca tccagattcg ctttgaggcc
     1021 gaaagtgaaa taggaacata acgaagctga cggaggcggt cacggctttc ccgtgggcgt
     1081 tttcgtcagc agacaagccc gtcagggcgc gtcagggggt tttagcaggt gtcggggcgc
     1141 agccatgacc cagtcacgta gcgatagcgg agtatatact ggcttaatca tgcggcggca
     1201 gagcaaatgg tgagagaaca gcgacatatg aggtgtgaaa tgctgcacag atacgtaagg
     1261 agaaaatgca gcttccggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1321 gttcggctgc ggcgagcggt gtctgctcac gcaaagacgg atatacggtt atccacagaa
     1381 tcaggggata acaccagaaa aaacatgtga gcaaaaaaga ggccccgaaa aaaggccgcg
     1441 ccggtggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa ccgacgctca
     1501 agtcagaggt ggcgaaaccc gacaggactt aaagatacca ggcgtttccc cctggtggct
     1561 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     1621 cgcgttcgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
     1681 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1741 cttgagtcca acccggtaag acacgactta acgccattgg cagcggccac tggtaaacag
     1801 gatgtgcgag agatgtattg agagttcttg aagtggcagc cttactgagg ctacactaga
     1861 aggacagatt tggtgacatg tctcgcacaa gactgttacc acggttcaga agttccccaa
     1921 cttactgaac cttcgaaaaa ccacctcccc aggtggtttt ttcgttttct agagtagaag
     1981 actacggctg taagggaatg atctcaagaa gatcctttga actttttata aaagttgctt
     2041 ataaaatgat gcatcagcac aacttttact caatcacact catctttctg gcctccatgg
     2101 catgacgtaa aagtatatag ccacatgcaa gcatccctcc aatgacagaa gaaagaatta
     2161 caattatggc attacttggc ccatcctttc tgaccggtaa cgttgggctc aattgatact
     2221 gaaatggcat aaaatcaaca tcccccactt tcgccacaac caattgattc acaacatatt
     2281 gacgatttcg caactcccca ctcaattccg aaacatcctt gattgatttt ttgatgttca
     2341 atttagttgc tataccatca gaaccgagag ccacaggaaa atctgggtca tccttcataa
     2401 tctgaccatt gctgtataca ggttttttta ttccagccgc attagcaacc tccagtgaat
     2461 aattgaggcg tcgaatatct gcattcagct ggtttctaat ttttatctca tccagtgcaa
     2521 gaatttcttt ttcaacatta gttttaactt ctagtttatt tcttatatct tccatcaaat
     2581 cccttacaac aagggaagaa acataattaa tatacccaga caacacatcg tgagcttctt
     2641 tacttgttgg agcagtaaaa ctcagagtcc atgatacata caatgatgac ttgtcgtttt
     2701 tcttactggc attatcatca atcgacttca tatttttact caactcaaca attgcacgat
     2761 gcagttcgag agcgctaatg ttattctttt taataatctc cataacataa ggagatgatc
     2821 tcatatactc attcaacgaa ttaacggact gaaatttctt aataaacaga gcaaatgcgc
     2881 tatcacgatt gatatcaaaa tccactccca gaacatgaag cttggaaagc tccttattta
     2941 attcactcca ttgaacagac tcagcaggag taaccacagc agaactagtc catttttgtg
     3001 gcaaaacaaa agaaacaaga aagccaatac aagcaaaaaa tagaatcacc ccaacaattt
     3061 ttatttttgc tgcccataaa acgctaatca agccaaaaag gtcaatttct tcattaccat
     3121 tcggcaacat gttgtaatta gaaaaatgtt cgtcagagac ctttgagatt ttcattg
//
LOCUS       DAJVQH010000231         3144 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJVQH010000231 DAJVQH010000000
VERSION     DAJVQH010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.234"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="OQM87_004119"
     CDS             complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="OQM87_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCS1510019.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQ"
     gene            complement(1002..1097)
                     /locus_tag="OQM87_004120"
                     /pseudo
     CDS             complement(1002..1097)
                     /locus_tag="OQM87_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="OQM87_004121"
     CDS             complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="OQM87_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCS1510020.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="OQM87_004122"
     CDS             complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="OQM87_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCS1510021.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2194..3144)
                     /locus_tag="OQM87_004123"
                     /pseudo
     CDS             complement(2194..3144)
                     /locus_tag="OQM87_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          781 a          762 c          764 g          837 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc cgtgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggcttgcag agcatgggat gataatgggt tatcactaat atgtattgaa
      121 cattcattcc tcagattaag aatgctttcc gggatatgac ttatctgatt tctatcaaaa
      181 aaatactctc tgacaacggg attatttccc tcagaaaatg ctggtagctc agtgaggaaa
      241 ttacgggtcg ctctgagatt tttaagagcc tgtgggagcg atggtaagga gactatttcg
      301 ttatagctga tattcatagt caataacgaa tcgggtaaag ctggcaggtt ttccagctca
      361 ttgtgttgta cctgaagtga ctccagcgca ggaggtagca cgggcaattc tgataattta
      421 ttgctggcgg aatataattt ttttagcgta acaggcagtt caggtaggtt agttaattga
      481 ttatatgata cattgagcag cgttatctga gctggtaagt tgtcaggtag cgaggacaga
      541 tttaaacggt ccagtcgaag ttcatcggaa ttattgataa gacattcttt aagtcgggag
      601 acagcctcat cacgctcttc accggggagc gcttgttttt cccatttatc ccatgctgaa
      661 aagtaatcag catatgtacc ggaaatagtg ttataaaaag aattttgggg caatgaaaag
      721 ttattattta tcggtaacat gatgggcatc cagttaaagc cggtggacaa atggctttgt
      781 taaaagaaat aagtaggagt ttttatgaag ttcattccat gaaatgaact tcagcacttt
      841 tttattataa taaggataaa caaaattggc tgtaaaaaaa cgatactgtt tcagttaacc
      901 gtgaatggcc tgtttgcacc gtattgttct tgtttttcgg ttagttaaac atcgtacttc
      961 atatttgaac ggtaagcggc tcgtcagaac cgtattgata tttactgaga ggtcagatca
     1021 actttccagg gcaacagatc gcgtacccgg tttgccggcc agtcctggat atgctcaatg
     1081 acgtaacgca gccattttgt caacgccacg atttttgacc ctagcctgac atttatattc
     1141 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     1201 cttcttcccc gataacgaag atcggcacac tggccatatc agtggtcatc atgcgccagc
     1261 tttcatcccc gacatgcacc accgggtaaa gctcacggga gactttatct gacagcaggc
     1321 gggcactggc cagggggatc accatccgtc gtccgggcgt gtcaataatg tcactctgta
     1381 catccacaaa cagacgataa cgactctctc ttttataggc gtaaacctta aactgcattt
     1441 caccagtccc tattctcatc ggcaaaagag ccgttcattt caataaaacg ggcgatctca
     1501 gccatcccct gctgattttc agcctgccag cgttccgcac gcagacggcg ggcctctttt
     1561 tgcatggccg tatttaccag gcctgagata ttcacgtttg cggatttaag taactgatag
     1621 ctgtcactgt ctatagtgac agtaatacgc tgcttcatga taagccctca aaaacatact
     1681 tatgatatgt atatgagtat atattaatgg tacaaaccat caatatccat aaaaagtaca
     1741 ccattaattc gctataagtt tttaatacaa ttttttctgc tgcgattttt ttatactata
     1801 attagcatac ttataacacc gtaagtaaat tacatgaaag caggcacagc gtgatccgat
     1861 ttttctgcgt ataacgattt tccggaagtg taatacggtt gcgccccggg ccgggcaccc
     1921 ttttctgttg ttgtgtacgg gcccacagag aatggtaccg gaggcgggct cagaagtact
     1981 gagtcgggtg atcgcgtcag gccgcaaagt actgacacgg gtattggtga ctggtgatga
     2041 agtactgccc cgggtatgat ttcaggctga ttcaggttct gattctctgt cagtccgcgt
     2101 ggttgctggc ctggctgatt tcctgttgtc aggcgttcaa caaccgactg caggttatag
     2161 cccggttcac gaacacctga cggcaggtat cactcacccg gtgaaggtaa atccctcaag
     2221 aggcagcaac tctgccagtc gctcctccgg ccattccggc agacgcgtca ggacgtccgt
     2281 cagccaggca tgtggctcca gaccattacg ttttgctgtt tccagaaggc tcattatctg
     2341 tgccgcccgg tttccggcca tcagtgaacc ggcgaacagc cacgctttcc gtcccataac
     2401 aaccgggcga atggcacgct cgcagatatt gttgtccagg ggcaccgctc cgtcacccag
     2461 gaagcagctc agttcatcgc gccggttcag aatatagtta atcgccttat ttaatggccc
     2521 gtcgggcgga cagcacggct cctgctcttc aagccaggca aacaggtcat tcactatcgg
     2581 ccgggagtac cgttatcgcc actgccgtat tttttccaca gggcgctcac ggatgagttt
     2641 ctcaatgcgg taaagacccg cgatttttct cagctccacg cctgcacggg gctccctgct
     2701 ggccttatac agatttgcga agccgcgccc tgcgtgactc cagcaacagg ctgatgtgat
     2761 gccggggtga ttatccgcca gagatttgta cacgctgtat ccgtcactca ccagcgtacc
     2821 gccacaccag cactgcagcc aggtctcagg gtatctgagg gcccgtccgg tctgcgagtc
     2881 aaaacagacg accggcggac cgcttctttc accgctgacg taagcacaca gccagccgga
     2941 gcaggattta ccgcctttcc gggtatcaag gagcctcagg gacgtttcat ctgcatgaat
     3001 aacatcccgt gtcagaagct catggcgtag cagtttcgcc agcggggcca gtgcagctcc
     3061 ggcaacaccg accatatccg ccattgtact gacggggagc tccacaccca ttcgggagaa
     3121 aatgtgctgc tggcgataca gggg
//
LOCUS       DAJVQH010000232         3049 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJVQH010000232 DAJVQH010000000
VERSION     DAJVQH010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.235"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OQM87_004124"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OQM87_004124"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCS1510022.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OQM87_004125"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OQM87_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCS1510023.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3049)
                     /gene="bcsE"
                     /locus_tag="OQM87_004126"
                     /pseudo
     CDS             complement(1963..3049)
                     /gene="bcsE"
                     /locus_tag="OQM87_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
BASE COUNT          763 a          786 c          805 g          695 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aagcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgccctta
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctca
//
LOCUS       DAJVQH010000233         3039 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJVQH010000233 DAJVQH010000000
VERSION     DAJVQH010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.236"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            54..1085
                     /gene="ynaI"
                     /locus_tag="OQM87_004127"
     CDS             54..1085
                     /gene="ynaI"
                     /locus_tag="OQM87_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCS1510024.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(1136..2749)
                     /gene="mppA"
                     /locus_tag="OQM87_004128"
     CDS             complement(1136..2749)
                     /gene="mppA"
                     /locus_tag="OQM87_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCS1510025.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          791 a          707 c          671 g          870 t
ORIGIN      
        1 ccgccagggg tttccggcat gacgacaatt atctggagga gcctgataaa acaatgatcg
       61 ctgaactgtt tacaaataat gcgcttaatc tggtcattat tttcggtagc tgcgcagcat
      121 tgattctgat gagcttttgg tttcgccgtg gaaatcgtaa aagaaaagga tttttattcc
      181 atgcggtgca atttttaatc tacaccataa ttatcagtgc tgttggtagc atcattaatt
      241 atgttataga aaactataaa ctcaaattta tcaccccagg cgttatcgat tttatctgta
      301 cgtccctgat tgcggttatt ctgacgatta agctatttct gctgattaat cagtttgaaa
      361 aacagcagat taaaaaaggt cgcgatatca ccagtgcgcg gattatgtcg cgcattatca
      421 aaatcaccat tattgtagtg cttgttctgc tttatggcga acatttcggc atgagccttt
      481 ctggcttgct gacctttggt ggtattggtg gtctggctgt cggtatggcc ggtaaagata
      541 ttctgagtaa tttcttttcc gggatcatgc tctatttcga ccgtcctttc agtattggcg
      601 actggatccg ttcaccggac agaaatatcg aaggtacagt agcggaaatt ggctggcgaa
      661 ttaccaaaat tacgaccttt gataatcgtc cattgtacgt accgaactcg ctgttttcgt
      721 cgatcagcgt agaaaaccca ggacgaatga ccaaccgccg cattaccacg accattggtt
      781 tacgttatga ggatgcggca aaagtgggcg ttattgtcga agccgtacgt gagatgctga
      841 aaaatcaccc ggccatcgac cagcgacaaa ccttactggt ttatttcaac cagtttgctg
      901 actcgtcatt gaatattatg gtttattgct ttaccaaaac cacggtatgg gctgagtggc
      961 ttgctgcaca gcaagacgtt tatttgaaga ttatcgatat tgtacagtca cacggcgcgg
     1021 actttgcctt ccccagccag acgctgtata tggataacat tacaccgccg gaacagggtc
     1081 gctaaatcag acatcactgg cgttatccac aagggtaacg ccagttccca tcacatcaat
     1141 gcttcacaat atacatagtc cgactgtacg ctacatcttc agggttatta atgggataac
     1201 ctttcagcca cggtttgatt aatcgtccat tggtatattg ataaattggt gcaatcggtg
     1261 cttgctccat gaggattttt tctgccgcgt tgtaatcggc attacgcgct ttaacggtat
     1321 tttccgtcga tgcctgggcc agaactttgt cgtatgccgg attgttaaag cgtgaaatat
     1381 ttcctgaatg cattgacgtt aataatgtca ggaaagtgga gggttcatta taatccccca
     1441 cccatgaggc gcggataaca tcaaaattgc cggtgttacg gctatcgata taggttttcc
     1501 attcctgatt ttgcaattta acatctacgc caaggttctt tttccacatc gatgctacag
     1561 caatcgcaat tttttgatgg ttttctgaag tgttatacaa aagcgtcagc ttcagcggtt
     1621 tttgcggacc ataaccagct gcgctcaaca aagtttttgc ctgcgcattc agttcttcct
     1681 gactcatttg ttcaaacggc gaaggttccg gcgtaaatcc ggcggtaaca tctggtataa
     1741 aatgccacgc tggcttttcg cccgtcccca acactttttc ggtcatcagg cggcgatcta
     1801 tcgtcatact taatgccagg cgaacgcgct gatctgccgt cggccctttt tgcgtgttaa
     1861 aggcataata ataggtcccg agctgcggtg gcgtataaac ctgccccgga atatccttca
     1921 acagcttctg atacatattt ttcgggaagg attcggtgat atcaatatcc cccgcgaggt
     1981 aacgcttagt ggctgcggat tcctgattaa ttggcaggaa ggtcactttt tgcagcaccg
     2041 ttttggcgtt atcccaataa tgggtattcg gtaccacgac cagtttttca ttgactacgc
     2101 gctctttaag aacataagcg ccattgccga tcagatttcc gggtttcgtc cactctttac
     2161 cgctttctac gttggctttt tgcaccggga agaaggcaaa gttagcggtt aaattcacaa
     2221 accacggcaa cggtttatca agctgaattt tcaaagtatg ggcatcaact gcggtgacgc
     2281 caagctgatc aggcgtagct ttaccatcaa taatcgcctg tgcgttgttg attcccgcca
     2341 gcgcggcaaa ccatgcaaat ggcgacaatg tttttgggtc caccagacgt tgccagctgt
     2401 agacaaaatc ttgcgccgtt accggtgtgc catccgccca ttttgcgtta tcgcgcaggg
     2461 taaaagtcca gatacggttg tcattacttt tccattgagt cgcgacgccg gggacaatct
     2521 cccctttttc gttctgattc accagacctt caaacagatc gcgaatgacc tgaatctctg
     2581 gcaggcccac ggctttagcg ggatccagcg acgcaggctc atctttaatg tggcgcacca
     2641 gctcctgctt ctctgccagt actgtgccgc tcggaacttc tgcagcatac gaaagagaaa
     2701 tgctgctgac caacagcgca caacacgtaa ctgaaacaga gtgcttcata agtttccctc
     2761 taaccgacat taaaattgat gcgtaattat ttgtttcgcc ttaaagaaat gcaaacaact
     2821 tccatcaaaa aagtgacaaa ggaagcatca aatgttttaa gtatcaatat atcaaatcag
     2881 atttagtgta aataatagac gccaaaatgc ggcaaagcac atagaacacc gaacactacg
     2941 ttcgggcttc gcacgttcaa accagttttc ggtatcataa tcagcatctg tcgccattgt
     3001 acgaagaata aaaaataacc aaaacgttga aaggaactg
//
LOCUS       DAJVQH010000234         2955 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJVQH010000234 DAJVQH010000000
VERSION     DAJVQH010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.237"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            133..2001
                     /gene="kup"
                     /locus_tag="OQM87_004129"
     CDS             133..2001
                     /gene="kup"
                     /locus_tag="OQM87_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCS1510026.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2168..2587
                     /gene="rbsD"
                     /locus_tag="OQM87_004130"
     CDS             2168..2587
                     /gene="rbsD"
                     /locus_tag="OQM87_004130"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCS1510027.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2595..>2955
                     /locus_tag="OQM87_004131"
     CDS             2595..>2955
                     /locus_tag="OQM87_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1510028.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYV"
BASE COUNT          658 a          704 c          775 g          818 t
ORIGIN      
        1 gctttttctt tgagccaggt taatgtggcc gcatttagga gtacgatttt gccgttaatc
       61 gtgcatactg tgcgcttttt tgtgggccaa gggactaagc acacatttca tatttcaacg
      121 aaagactagt ctatgagcac tgataataag caatcattgc ccgcgattac cctcgcggcg
      181 attggagttg tctacggcga tattggtacc agcccgttat atacacttcg tgaatgtttg
      241 tccggccagt ttggttttgg cgttgaacgc gatgccgtgt ttggcttttt atcgctgatc
      301 ttctggctgc taatctttgt ggtttccatt aaatatctca ccttcgtgat gcgggcagat
      361 aacgccggtg agggggggat cctgacgttg atgtcgcttg ccgggcgtaa tacgtcggcg
      421 cgaaccacat caatgctggt gattatgggg ctaatcggcg gcagcttttt ctatggtgaa
      481 gtcgtcataa cacccgctat ttcggtgatg tcagccattg aaggtctgga aatcgtcgcc
      541 ccgcagctgg atacctggat agttcccctc tcaattatcg ttctcacttt attatttatg
      601 attcaaaagc atggcacggc tatggtcggt aagctgtttg cgccgatcat gctgacctgg
      661 tttttgattc tggcaggact ggggttacgt agcattattg ctaacccgga agtgctgcat
      721 gcgctgaatc cgatgtgggc ggtgcatttc ttccttgaat acaaaacggt ttcttttatt
      781 gcattagggg cagtggtgct gtcgattacg ggggtcgagg cgctgtatgc tgatatgggg
      841 cactttggta agttccctat tcgcctggcg tggttcaccg tcgtattacc ttccttgacc
      901 cttaattact tcggtcaggg agcgttgttg ttaaagaacc cggaagcgat taagaacccg
      961 ttcttcctgt tggcaccgga ctgggcgctg atcccactgc tgatcatcgc cgcactggcg
     1021 acggtaattg cctcgcaggc ggttatctct ggcgtcttct cattgacgcg tcaggcggta
     1081 cgtctgggat atttgtcgcc gatgcgcatt attcacacct ccgaaatgga gtcagggcaa
     1141 atctatattc cgtttgtgaa ctggatgctc tatgtcgcgg tcgtgattgt gattgtcagc
     1201 tttgagcact ccagcaacct ggcggcggcg tacgggattg cggtgaccgg aaccatggta
     1261 ctgacgtcta ttctctcgac taccgtggca cgtcagaact ggcactggaa taagtatttt
     1321 gttgcgctga tcctgattgc tttcctttgt gtcgatattc cattgttcac cgctaacctc
     1381 gataaactgc tctccggcgg ctggttgcca ttgagcctcg gtactgtgat gtttatcgtg
     1441 atgaccacct ggaagagcga gcgtttccgc ttgctgcggc ggatgcatga acatggtaac
     1501 tctctggaag cgatgattgc ttcgctggag aaatcaccgc ctgttcgcgt gcccgggacc
     1561 gcggtgtata tgtcgcgtgc aatcaacgtc attccctttg cgctgatgca taaccttaaa
     1621 cataacaagg tattgcatga gcgggtgatt ttgttaactc tgcgcaccga agacgcgcca
     1681 tatgtccata acgtccgtcg ggtacagatt gaacaactgt cgcccacttt ctggcgcgtg
     1741 gtggcaagtt atggttggcg agaaacgcca aacgtggaag aagttttcca ccgctgcggt
     1801 ctggaaggat taagttgccg gatgatggaa acctccttct ttatgtcgca tgagtcgttg
     1861 atcctcggca aacgcccgtg gtatttgcgt ctgcgcggca agctgtactt gctgctgcaa
     1921 cgtaatgcgc tgcgtgcacc agatcaattt gaaatcccgc caaacagggt tatcgaactg
     1981 ggtactcagg tcgaaatcta acgccagacg cctcctttct tcataagggg gcgtttttgt
     2041 tttcatggtt aatcaccatg taaaacgttt cgaggttgat cacatttccg taacgtcacg
     2101 atggttttcc caactcagtc aggattaagc tgcggatcag cgaaacgttt cgctgatgga
     2161 gaaaaaaatg aaaaaaggca ccgttcttaa ttctgatatt tcatcggtga tctcccgtct
     2221 gggacatacc gatacgctgg tggtgtgtga tgctggttta cccatcccca aaagtacaac
     2281 gcgtatcgat atggcattaa cccaaggtgt accttctttt atgcaggtgc tgggcgtcgt
     2341 cacaaatgaa atgcaggtcg aggctgccat tatcgcggaa gagatcaaac aacataatcc
     2401 gcaactccac gaaacgttgc tcactcacct tgagcagctg caaaaacacc agggaaatac
     2461 cattgaaatt cgttacacca cgcatgaaca attcaaacaa caaaccgcag aaagtcaggc
     2521 ggtaattcgc agcggagaat gttctccgta tgcgaatatc attctctgtg ctggcgtgac
     2581 gttctgaggc cgtcatggaa gcattacttc agcttaaagg catcgataaa gccttcccgg
     2641 gtgtaaaagc cctctcgggc gcagcgttaa atgtctatcc gggccgtgtg atggcgctgg
     2701 tgggcgaaaa cggcgcgggt aaatccacca tgatgaaagt gcttactggt atctatactc
     2761 gcgatgccgg cacgctttta tggctgggga aagaaacgac atttaccggg ccgaaatctt
     2821 cccaggaagc cgggattggg attatccatc aggaactgaa cctgatcccg cagttgacca
     2881 ttgccgaaaa cattttcctc ggtcgtgagt ttgttaatcg ctttggcaaa attgactgga
     2941 aaaccatgta tgtcg
//
LOCUS       DAJVQH010000235         2867 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJVQH010000235 DAJVQH010000000
VERSION     DAJVQH010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.238"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..291)
                     /locus_tag="OQM87_004132"
     CDS             complement(<1..291)
                     /locus_tag="OQM87_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCS1510029.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAF"
     gene            complement(338..1057)
                     /gene="yehT"
                     /locus_tag="OQM87_004133"
     CDS             complement(338..1057)
                     /gene="yehT"
                     /locus_tag="OQM87_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCS1510030.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1054..2739)
                     /gene="btsS"
                     /locus_tag="OQM87_004134"
     CDS             complement(1054..2739)
                     /gene="btsS"
                     /locus_tag="OQM87_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCS1510031.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          743 a          765 c          654 g          705 t
ORIGIN      
        1 aaacgcgatg cgtaattttt tcagcacgat gttgttatta atgcgacgtt ccggctccag
       61 tgccggagca gactcatcct tgccgcgttt ttcataaatc aggccattga ggaatgacga
      121 caaaacaatg tccggacaac gctgaaaacc ctcttcgtct tctttacgta gccagacggc
      181 gatctgttcc gcggtggctt cgacattatc cagcgccaga atacgcacca ggtcattatt
      241 attggctttc aaaatgtagc gcacgctgcg cagaatatcg ttacttatca tgaggccttc
      301 aggtgttgat gaggcaaaaa gccattttag cagtctttta caggccaatc gcctctttta
      361 agcttttcag ataacggcgg ctgaccggca cggttaagcc attacgcaaa atcaactcgg
      421 cctggccgtt atcttccaga cgaatctcct gtaaatgcgc gaggttaacc agatactgac
      481 gatggcagcg cagtagtggt gtacgacttt ccagggtacg taatgtcaat tcggtaaagc
      541 cctctttccc ttcgtggctg gtaacgtaga caccgctcat ccgactgctg acaaatgcca
      601 catctttcat ttgcagcaaa taaatccgac tatgccccgt acaagggata aatttcagcg
      661 cctgttgatt ttccggtaac agcgaaacat cctgcttgct gcgctcctga cgcaatcgcg
      721 ccagcgtttt ctccagtcgc gcttcatcaa ttggcttcag cagataatca aaggcatgtt
      781 cttcaaaggc tttaatagcg tattcgtcaa acgcagtgag aaaaacaata tacgggcgat
      841 gttccgggtc aagcatcccc accatttcca gaccactgat gcgcggcatc tggatatcga
      901 gaaacagcac atccgggcgc agtttatgca ccgcgccgat cccttccacg gcgtttgaac
      961 actctccaac gatttcaata tcgctctgct cctgcaaaaa tatacgcagg ttctcccgtg
     1021 ctaacggttc atcatcgaca attaagactt taatcatgcc tcgtccctcc atggtagtcg
     1081 taacgttatt cgggtgtaac tatcaggctc acaggcgacg cttattccat agtcatcgcc
     1141 aaaccgttca cgtaaacgct tatccaccag attcatcccc agcccactgg cattggttac
     1201 cggttgatac aaaccggcat tgtcttcgat ctccagcatc aaatgttgcc cctcacgtcg
     1261 ggcgctgatt gccactcgcc ctgtatccag cagttgtgat gtcccatgtt taatggcgtt
     1321 ttccactatc ggttgcaggg taaacgcggg caattgctgc tgggataatt cttgcggaat
     1381 agcaatgttg acctgcaacc gcgactggaa gcgcgccttt tcaatttgca gataagcgtt
     1441 cacatgttca atttcgtcgg cgagagtaac aaactccgaa ggccgcttta agtttttgcg
     1501 gaaaaaagtg gaaagatact gcaccagctg gctggcctgt tcgctgtcgc ggcggatcac
     1561 cgctttaatg gtgttaagcg cattaaacaa aaaatgagga ttcacctggg cgtgaagcag
     1621 tttgatttct gactgggtga gcatcgcttt ttgccgctca tattgcccgg caagaatctg
     1681 cgccgaaagc agttgcgcaa tcccctcgcc cagcgtgcgg tttattgaac tgaataaacg
     1741 gtttttggct tcatacaatt tgatggtgcc catcacgcgc tgattttcgc cacgcaacgg
     1801 aattaccaga gtcgacccca gtttgcattg cggatgcaaa gagcaacgat aaggtacttc
     1861 gttgccatca gcgtagacca cttcaccggt ttcaatcgct tttaaggtgt aagtcgaaga
     1921 aatcggtttg ccgggtaaat ggtggtcgtc accaattccg gtaaaggcca gcaatttctc
     1981 tcgatcggta atcgcgactg caccaatatc cagctcctga tacagcacct gagccacttt
     2041 catgctgttc acttcgttaa acccctgtcg caaaatgcct tccgtcgagg ctgccacttt
     2101 cagcgcagtg gcagaaaaag ccgaagtgta tttttcaaac atcgcgcgtt tatcgagcaa
     2161 tatacgcata aacagcgccg cgccgacggt attggtgacc atcattggcg cagcaatatt
     2221 actcaccaga cgcaccgcat cttcataagg tcgggcgatc gcaaggatga tcagcatttg
     2281 caccatttca gcgacgaacg tgacggcacc ggcggtaatg gggttaaaga ctttatcagt
     2341 gcgcccgcga cggatcagga tgctgtgtac caggccgccg agcaatcctt caacgatggt
     2401 tgagatcatg caacttagcg cggtcatgcc ccccatcgaa tatcgatgta agccgccggt
     2461 cagaccaacc agcccaccga cgaccggacc gccgagtaag ccgcccatta ccgcgcctat
     2521 cgcacgggta ttggcaatag aatcgtcaat gtgcaaccca aaccaggtgc ccatgatgca
     2581 gaagatggaa aagacgatgt agcagagaaa tttatgcggc agacgaaccg tgacctgcat
     2641 taacggtatg aataatggcg ttttactcat taaccacgca atgactaaaa aaacgcacat
     2701 ctgctgaagc agcagcaaca ccagattaaa atcgtacata cccgcaaacc acacttccct
     2761 ttaaaacgcg taacatacat tgcctgcgtt taactttctt tgaactcttg cagaaaaatg
     2821 agaattcgtg agtacgatca ctcaaaatcg cctggcaaaa ataaaat
//
LOCUS       DAJVQH010000236         2834 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJVQH010000236 DAJVQH010000000
VERSION     DAJVQH010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.239"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..420
                     /locus_tag="OQM87_004135"
                     /pseudo
     CDS             1..420
                     /locus_tag="OQM87_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1004..1720
                     /locus_tag="OQM87_004136"
     CDS             1004..1720
                     /locus_tag="OQM87_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510032.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSYKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2272..2676
                     /locus_tag="OQM87_004137"
     CDS             2272..2676
                     /locus_tag="OQM87_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510033.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
BASE COUNT          817 a          543 c          627 g          847 t
ORIGIN      
        1 catgatcggg gaagacccag atatgaacag gcacattcag agttacttta aagagcttac
       61 tgaacgtctg aggtgttaga gccatgactg ccgcagcatt gctggcggaa atgcctgaat
      121 cgagcagcct ctcgagacgt gaaatcagcg cacttgtcgg tgttgctcag gtaaaccggg
      181 actcgggaac tctgcgcggt cgacgaacca ttttcggtga ttgtgctggg gaagaacagc
      241 tctgtacatg gcggcgcttg aggcccccgg tttaatctgg tgataaaggc gttttacatg
      301 cgcctacttg cagccggaaa cgccaaaaaa gtggcactgg ttgcctgtat gcgtaagttg
      361 ctgaccatta tgaacgcaat gctcagaaaa aacgaagaat ggaatgaatc gtatttatga
      421 aaactggatt gtgggccata aatattccaa aaagatagcc cgctccgttt attaccggaa
      481 cgggcgagag catgacattg tctgttatta taatgagact gctgattcta cttattttta
      541 atttcaagaa aagcgttttc tacaaagaat tatgagattt aatgttttgt ttagacaata
      601 ttatcattct gttcaataat gaaagtcttc atcaacaaca agtaaaaaca acgtccctct
      661 catcgaaccg gacttgcaga tttcccgcat ccggctctct tgcaacacat cagaccttca
      721 cgttcgggcg gttatgggga aggtccagca agctgtatga gccctaactg tttgaatacc
      781 agtagattac cagaaacttc tatcgattga agacattacc aactagcgtt actaaatgtt
      841 gaccaagttt tgttcacttg tctggttttg atagtagcat tctgcagagt attttgatat
      901 gataaatatg cattaacaat gatattattg ttaatttcct agcataaaaa ttaaaagatc
      961 aacttttatc tcgaatattt tttacattat cagttggttt cttatgaatg acaattcatt
     1021 gcttagaaat tcttcattat tcatcgccta tatgggatgt gtagggtggg tcagtgctta
     1081 ttcttatggc tggggaacct ctttttacta tggatttcca tggtgggttg ttggtgcagg
     1141 tttggatgat gttgccagaa gtttactcta tgctatcata gtaatgggta tactttttac
     1201 cggttggggg attggtgttt tattttttct tcttataaag aaaagaagta aaatacaaga
     1261 cttgagtttt tttagactat tttttgctat taccctactg tttttccctg taatattcga
     1321 acttttaatt ctcaaacaat atttcatact tccattatca ctttcatgca ttattagttc
     1381 attggttatt tcaataataa ttcgtatata tggacggatc ttttctgtat catgtttttc
     1441 agatatccct tttgttagaa aacaccgcat taagttaatt atggctggtt ttttggttta
     1501 tttttggctt ttttcctttc ttgttggctg gtataaacct caattaaaaa aagaatatca
     1561 gatgctttgt tataataata gttggtatta tgttcttgct cgttatgatt cacggctcgt
     1621 tttgtctagt tcatataaag atgacagtaa cagattctta atatttaata cagaacagtc
     1681 gggattttat gaaatcaatg atgtttatgt cagaaagtaa taatcctctc ccacaaagtg
     1741 aattaaatga atatataatg cgttcgaagt atatgtattg agctgtcata ctgagaaaca
     1801 aaccttaggg cagcacaatt aaagccagaa acaactatgc aagaaagtta ctaaataagt
     1861 gctcggaaca gtaaaacact caggggggac tcagtccgga ttatgtaatc agataaattg
     1921 ccaaacatca caaatcacca gccggactga gtgatgccga gcattgcacc aataccccgt
     1981 gatgaacgat gcctgatgca gaaagctccc tataaaacaa agaacaaaaa ctacgaccgg
     2041 cgattcaccg tcatactgat gctgcatcgt ggtgacactg tctgtcactt tcatgttgac
     2101 tgaacaactc tgctgcgaag aaggtgtacc gccggcggga tttgattgtc cggcgtctag
     2161 cttccacttc cctgatggaa ctgaaagttg ttatggccgg tcagcttgcc ctgtccgcag
     2221 gacatgaggt gctgtgacgg acagcaccag taacggaaag tgacaatgaa aatgaatact
     2281 gcgctgatag ttgcactaat gtgtatgtgg tatgcggtac ccgctgctgc aaaagagact
     2341 ttactggcta tgccacgtaa ttcaacagaa cattgctatg cggagattaa tgtgcacgga
     2401 ccatatgggg tgtatttccg agtggtcccg catccccccg ggggaaagag ctgggtcgaa
     2461 tgtaacagtg actactatta ctctgataaa ccaccgggag tacagatact tggaacccgt
     2521 gcagggtgtc gggtatacgg aatctgcggg accacctcga cactgcatgt ggctggaaga
     2581 ggggttgtgt gtattaaaaa tatatgcagt ccacgtggga tgataattca caggatacgg
     2641 aaacgtcctg tagtggctgt aagcgatgag atgtgatctt acccagcaat agtggacacg
     2701 cggctaagtg agtaaactct cagtcagagg tgactcacat gacaaaaaca gtatcaacca
     2761 gcaaaaaaac acgtaaacag cattcgcctg aattttgcag tgaagccctg aagcttgctg
     2821 aacgcatcgg tgtt
//
LOCUS       DAJVQH010000237         2721 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJVQH010000237 DAJVQH010000000
VERSION     DAJVQH010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.240"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            368..2020
                     /locus_tag="OQM87_004138"
     CDS             368..2020
                     /locus_tag="OQM87_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1510034.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="OQM87_004139"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="OQM87_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2334..2453)
                     /locus_tag="OQM87_004140"
                     /pseudo
     CDS             complement(2334..2453)
                     /locus_tag="OQM87_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          812 a          552 c          628 g          729 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc gtcatacggc
     2521 tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gtaaatagct tcggatactg
     2581 tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt atttagactg
     2641 catccactta agcaaacgtg actgcattgc actccacagt cgatagctga agtttctgga
     2701 ccagaacttt ccatagtact c
//
LOCUS       DAJVQH010000238         2491 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJVQH010000238 DAJVQH010000000
VERSION     DAJVQH010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.242"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..646)
                     /gene="yidZ"
                     /locus_tag="OQM87_004141"
     CDS             complement(<1..646)
                     /gene="yidZ"
                     /locus_tag="OQM87_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCS1510035.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(621..1796)
                     /gene="mdtL"
                     /locus_tag="OQM87_004142"
     CDS             complement(621..1796)
                     /gene="mdtL"
                     /locus_tag="OQM87_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCS1510036.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1928..>2491)
                     /locus_tag="OQM87_004143"
     CDS             complement(1928..>2491)
                     /locus_tag="OQM87_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCS1510037.1"
                     /translation="SVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVK
                     SFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLT
                     FLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLI
                     PAIVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          712 a          664 c          636 g          479 t
ORIGIN      
        1 aggtgtcgct ctgttcccag caaatgctga tatgcggata acgtaaaaag gtgtccagat
       61 tccacgtttc atgcagtgcc ggatgatctt tgcgtaacca gacgcagggt acatcactaa
      121 acagcacttc ataatcaatg gctaacggta gcgagcttaa cagctcccgc gagcgaggat
      181 ggctttcgcg accggaaaaa ccaatatcca cttcaccacg agtaatggca tctaaggaat
      241 cgtaatccca gttacgtagt ttgatggtcg cctgcgggta acgttggtag atccgtttcg
      301 acagcgcatt aagcatgatc atcatcagcg gcgattccgc cgccaactca aacttcaggc
      361 cgcgcggtgt ctggtggtgc ggtttatcca gcagcagatt gctcatttgc atccattccg
      421 ccagattttg ctccatgctg accattagct gtgtgggcga cagccccagc ggtgagttca
      481 caaagagcgg gtcgtcaaac cacgctctta actttgccag cgacttactc accgctgaag
      541 gggtcacgtt catccgcttc gccgctttgg tcacgctgcg ctcctgcatc agcagttgca
      601 gacagagcag cagattgaga tcaagcgtgg tgatggattt cttcatgagc ggcaacgggg
      661 cgtccaggcg cgacgaacat aatcagcaac aggctcacta tgctacaggc aatcagaatc
      721 ccgatcagca tattccatgc gccgatacca accaccgcgg ccagccaaat ccacagtgac
      781 gaaccgcaaa cctgcgcaat acctaaggtc gagctggcta cgcccgcgcg taatgaaaat
      841 ggtcctaacg cctgactcat cgctacgcca aaacctaccg agaaaccggc gcaaatcagc
      901 gtgataccaa acagagaaac cgcatgggaa ggtgaaacgg caagggtgat ccccgccgca
      961 aggaataaca cctgcgaggt gatcatcaac gtacgtggct taaaaattcc cagcgcaaat
     1021 ggcgtcgaga atgaaaaggt catgctgacg ccagcggtca gcgccataat ggtggcgtat
     1081 tctccgcgct caaaccccat gatttccatc agcaataccg gcgatgtatt gacgaaagtg
     1141 aggatcaccg aaacgctgag ggtggtgata acaacacggc tgaggaaaaa acgattaagc
     1201 agcgactcgc tattttctcg tggtttgtcc gaagcagcgg gggacgctgg gcgcgtttct
     1261 tttaaaataa acaaagacaa catcagtacc gcgatgccca tcattgccat cgcccagaac
     1321 agactctgcc acgggaattt aagcataatc agatgtccga gcactggcgc taacaccgga
     1381 atgatgcagg taataccgtt gagtaatgac agcactttag cccgacgtcg atcatccagc
     1441 gtgtcgcgca aaatggcgaa cgccaccacg taacaacagc ctgcgcccaa cccctgtaga
     1501 aatcggcctg caagaaataa cgtgctggtt tcagccagtg aacagaacac cgaggcaata
     1561 ataaatagcg ccgcgccggg tatggcgacc ggctttctcc ctgaacgatc ggccacttta
     1621 ccggcaaata gcatcgcagc tgccatcccc gccagatata cggagaacgc aatatgcaac
     1681 tgcgcttcgc tggcattgag atcggcggcg atgcgcggta aaccaacgag gtacatatca
     1741 atcccggcgg gataaagtaa aaccagggca aaactacaaa tcaaaaagcg ggacatgggc
     1801 taaagcgtcc tgatagtaag gtgacgcaaa gcatacgtgg tgaaggagga taaggcgagt
     1861 tgccatttgg acataagtga tttcctgaga ggcaagaagc cagcgaatgg ctggcttctt
     1921 gaaggattta gccaaattta ggtaacacgt taaagacgtt gccgaaccag cacaaaatca
     1981 cggttatacc aaagagaatg acaatcgccg gaataagatt gccgccccag accgtgaaca
     2041 tctgattggg aaacttcttg cgagctttga ttgcaagcac tgcgggaata atgaccgccc
     2101 agatggtggc gcacagcccg gcaccgccga tcccgtaaat aaagccgttc gggaagatca
     2161 gatacaacaa cgcaggtggc aggaaggtta acagcacggt tttgaaacgc ccgccgtggg
     2221 agttatcaat cttaaacagg tccgccagat aatcgaacaa ccccagcgtg acaccaaaga
     2281 acgaactggc aacagctaag ttagagaaca ccagcaggca aaactcgata atgccgtgct
     2341 gtttggtgcc gaggaacgat ttcaccagcg aatcaacgtt gccgcctgag gagattatcg
     2401 ccttaaagct ttcgcgcgga atattcccca tcgtgcaata gagccagaag agataaatca
     2461 ccagcgccag cagcgaacca aataccacgc t
//
LOCUS       DAJVQH010000239         2488 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJVQH010000239 DAJVQH010000000
VERSION     DAJVQH010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.243"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..460)
                     /gene="intS"
                     /locus_tag="OQM87_004144"
     CDS             complement(<1..460)
                     /gene="intS"
                     /locus_tag="OQM87_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCS1510038.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(622..696)
                     /locus_tag="OQM87_004145"
     tRNA            complement(622..696)
                     /locus_tag="OQM87_004145"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(661..663),aa:Arg,seq:cct)
     gene            complement(772..1704)
                     /locus_tag="OQM87_004146"
     CDS             complement(772..1704)
                     /locus_tag="OQM87_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCS1510039.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMVIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1998..>2488
                     /locus_tag="OQM87_004147"
     CDS             1998..>2488
                     /locus_tag="OQM87_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HCS1510040.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPVRNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGG"
BASE COUNT          585 a          612 c          597 g          694 t
ORIGIN      
        1 ttgcaccgcg atcttcaaat ctgcggatta cttccagcag ttgcatcggc tcaatatcct
       61 gaatctcaag gccgccgatg atgggtaaaa tgtcgtcatc aaacattttt gcaagttcag
      121 tcgcataccc tactgaccag acttgcttct tgtgttcgta ccattccttg taaatcgcac
      181 taaatgaatt gttgttagac gaagcctttt tcgcctttac cggatcgatg ccagccgaga
      241 tgtctttcct cgcagtccat gctttatccc ttgcctcctg caaagtcata agcggatatt
      301 ttccgacggt caggattttc tccttaccgt caatcttgta gcgaagctgc catacctttt
      361 tcccggatac agggacataa aggtacaggc cattaccatc gagaaggcgg tatggttttt
      421 ctttcggctt tgctgcttca atctgcttaa cggtgagcat gggtaaaaat ccggtgggta
      481 aaattatttt atccactttt tacccgtcat ggagtgcggc tgtcaacgat ctgacgcgaa
      541 ccatgacgaa ctgtgaatat acggaaggct tgatattcag gggattttgc ggactggtac
      601 ggatgggagc gaactgataa atggtgtccc ctgcaggaat cgaacctgca attagccctt
      661 aggaggggct cgttatatcc atttaactaa gaggacaatg cggcatgagt atacccgcta
      721 atggactgcg gggtaagtac gctgccgctc gattgcttaa accctcgcca tttatgctgg
      781 gtttttatca tttttcttaa tgttttccgc acgttctgct ttttggcgtg cttctgcttt
      841 acgcttgttg ctcatgtcgt tacgaatctg tgcatgactc attaacgcga agataaaggt
      901 gccgccgcag atgttccccg ctaaagtagg tagtgcgaag ggccagatga aatcgctcca
      961 gtgcagcgta ccgttaaaca ccagatagag gatttcaaca gaaccgacca cgatatgggt
     1021 ggtgtcaccc agggcaataa gccaggtcat caatataatc accacaatct ttgccgcacc
     1081 tgctgcagga aacatccata ccatagtggc gatcagccag ccggaaatga tcgcgttggc
     1141 aaacatctcg ctgggggtgt tcttcatcac atccatgccg attttgacaa atgcatcgcg
     1201 agtttcttca ttgaagatag gcatatattc aaatgcccac gcagcaatac cagtcccgag
     1261 aatattaccc agcagcacga ctccccataa ccgcataagt aagccaacgt tgctcattgt
     1321 cggtttttgc atgacgggta gtaccgcagt cacggtgttt tcggtaaata attgctggcg
     1381 ggccattatg acgataataa aaccaaaggt ataaccgaga ttctccagca agaagctgcc
     1441 cggcacacct tccagttcga catgaaatat cccttttgcc agtaacgaag cgcccatcga
     1501 cagacccgcc gcaatggctg accacagtag cgccattgcg tcgcgttcta gctctttttc
     1561 accatcctgg cggatatgct catgaattac catcgctcgg gaggggagtc ggtcttcatc
     1621 tatttctatt tttttgccgc gctctttttc ttcgctctca acttcaattt cgtcgctgtg
     1681 ttgatcaatt ttgtcgttgt ccatggtctc ttctcttgaa ttagcacgta tagctaaagc
     1741 gtagcggctt ttttgctcgc aactggcggg agttactctg aaaatgtaga aaaggttgcg
     1801 tttgcctttt tctgtttcta tagaatcaag tagcctacag ggcggcgatt accaggctat
     1861 gatcaaatca gcaaatcagg gcgtctggac atcagttgac gtgctgttac aatcgcccac
     1921 acctaaacag gcggatacgg tatcgttccg tcatggatgg caaactgcat aagccataaa
     1981 aaaacaggga gacatttatg aagcttcgcc tgtcggcgct tgctctggga actacgcttc
     2041 tggtggggtg tgcgagttcc ggtacagatc agcaagggcg ttctgacccg ttagaagggt
     2101 tcaaccgcac catgtacaac ttcaacttca acgtattaga cccgtatatt gttcgaccgg
     2161 tcgctgtcgc ctggcgtgat tatgttccgc aaccggtgcg taatggtttg agcaacttta
     2221 ctggcaacct tgaagaacct gcggtgatgg ttaactactt cttgcagggc gacccttatc
     2281 aggggatggt ccactttacc cgctttttcc tgaacaccat tttggggatg ggcggcttta
     2341 ttgatgttgc agggatggcg aacccgaaac tgcaacggac tgaacctcac cgcttcggta
     2401 gtacgcttgg tcattatggc gtgggttatg ggccttacgt tcagttaccg ttctacggta
     2461 gcttcacgct gcgtgatgac ggtggtga
//
LOCUS       DAJVQH010000240         2412 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJVQH010000240 DAJVQH010000000
VERSION     DAJVQH010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.244"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(346..612)
                     /gene="ospE2"
                     /locus_tag="OQM87_004148"
     CDS             complement(346..612)
                     /gene="ospE2"
                     /locus_tag="OQM87_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HCS1510041.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(794..1195)
                     /locus_tag="OQM87_004149"
                     /pseudo
     CDS             complement(794..1195)
                     /locus_tag="OQM87_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1624..2412
                     /gene="virF"
                     /locus_tag="OQM87_004150"
     CDS             1624..2412
                     /gene="virF"
                     /locus_tag="OQM87_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCS1510042.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          741 a          433 c          446 g          792 t
ORIGIN      
        1 cccccctaag ccgtctatgg gaaggagtct gctgttattc cagagaatgg taattcagtc
       61 atatatttct ccatctatga cttgtcaata ccatcctgat atttgtgggt cagcaatata
      121 ttctactaaa aggtatttaa ggtcattgcg tccctgcact cgaataatgg tgaagaacgc
      181 atgataaata tatcacgata acgttcagta tgtatcgagg atagatggag ttactcctct
      241 ctgaaataga attactgctc tactttttct gtttccagaa attattagta acaaaccctg
      301 atatattatt ttgaaaatgg agcttaaacc agtctgaaat taacttcaga aatcagaata
      361 ccgttggctt tgtttttcat caattccggc ttcatttatc tttgtcaacc attgcatttc
      421 tgtagcagtg aatttcaacg ttttcgtccg gttggcagca ggtatttgta attcgtatat
      481 agctgctttt ttattgaaaa cagtatatcc atctgtagta acagtagaag ataataatac
      541 aggatttgat acttcaagga tgatattttc ttgtttttgt ggaaggcaag ggaatatagt
      601 ttgtgtaagc atattgcttc cttatgttat attttagtaa agaatttatt ttttacatta
      661 tagtcggcta aatgaagttt actgtaaaaa aacgatactt ttttcgtaag cgatgagatg
      721 tggagccaga aaaatggctg cgttacgtca ttgaactgtc cttgacccac gatattcaga
      781 cagtcgttgt atcttaagtt gacattaccc gctgacgtcg atattccctt ggtgagagat
      841 aacccaacgc actatgcgga tggttttcat tgtaatgctc gatcgccact gcaaggttat
      901 gcaatgccgt tcttggtttc ggcatgaacg cgatgcagtc ttccttcatc gttttcacga
      961 accgttctgc tattccatta ctctgagggc tactgactgc cgtcgtgcag ggctccagat
     1021 tcaactctct ggcgaactgc cgtgtttcat gagccgtata agccgaaccg ttatcggcca
     1081 ctggattggc cgttccggtc ctttatcgcc atatcactcc tgacatgaca tcctgcaccg
     1141 ttgctttgtc ataatctccc gtgcctgctg cccagtctat ggcttcacga tcgcaatatg
     1201 gaacgtaacg ctgtcatcgg gagaatcgtc aatgcggcat tcggttaacg cttaccaata
     1261 tcaacaccaa ccggatttgt ggagcctcca gtctgaaggg gctttatgcg ttccgtatag
     1321 gatattatga tgctggagtt ttgcgaagct ccactccttt ccgaacattt acacagatat
     1381 tgcctaagaa aagtgaaacc ataatatggt tatagtccct ttcagtgcaa atacttagct
     1441 tgttgcacag agaaatagaa gctgcataag ctctttcttc aaaaaatgta aataaagtta
     1501 aatataggaa aaattactta atctatctta ataacggaaa gattttgtat acaatcactg
     1561 ttacagaaat ttcttagtta ctctgtaaac actaaatata gtttggtata ttctgttgaa
     1621 tttatgatgg atatgggaca taaaaacaaa atagatataa aggttcgctt gcataactat
     1681 attattttat atgcaaaaag gtgttcaatg acggttagct caggcaatga aactttgact
     1741 atcgatgaag ggcaaattgc ttttatagag cgaaatatac aaataaacgt ctccataaaa
     1801 aaatctgata gcattaatcc atttgagatt ataagccttg acagaaattt attattaagc
     1861 attattagaa taatggaacc aatttattca tttcaacact cctattctga ggagaaaagg
     1921 gggttaaaca aaaaaatatt cctcctctct gaggaggagg tttctatcga tttgttcaaa
     1981 tctataaaag agatgccttt cggcaaaaga aagatctata gtttagcttg ccttttatca
     2041 gctgtttctg atgaggaagc tttatatact tcgatatcga tagcttcttc tcttagtttt
     2101 tctgatcaga taaggaagat tgttgaaaaa aacatcgaga agagatggcg tctttctgat
     2161 atttcaaata acttgaattt atcagaaata gctgttagaa aacgattgga gagtgaaaaa
     2221 ttaacatttc aacaaatcct tcttgatatt cgcatgcatc atgcagcaaa gcttttattg
     2281 aatagtcaaa gctatattaa tgatgtatca agacttatcg gaatatcaag cccatcttat
     2341 tttataagga aatttaatga atattatggt ataactccaa agaaatttta cttatatcat
     2401 aaaaaatttt aa
//
LOCUS       DAJVQH010000241         2347 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJVQH010000241 DAJVQH010000000
VERSION     DAJVQH010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.245"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..797)
                     /locus_tag="OQM87_004151"
     CDS             complement(<1..797)
                     /locus_tag="OQM87_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1510043.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLT"
     gene            complement(1398..1646)
                     /locus_tag="OQM87_004152"
     CDS             complement(1398..1646)
                     /locus_tag="OQM87_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1510044.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2001..>2347)
                     /locus_tag="OQM87_004153"
     CDS             complement(2001..>2347)
                     /locus_tag="OQM87_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1510045.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          609 a          432 c          561 g          745 t
ORIGIN      
        1 gttaatcttt gcagggattg cagagcatgg gatgacaatg ggttatcact aatatctatt
       61 gaacattcat tcctcagatt aagaatgctt tccgggatat gacttatctg atttatgtca
      121 aaataaccgt tccttaaatt ttccggcagg tatggaatat cggttaacat attgtcgatt
      181 gcccatatgg agtgaatgtt gtgaggtaga ggggggagtt cactaagaag attccctatt
      241 gcgctaatct cttgtaaaga aaatggtaaa ggtggtaaaa ctgccagccc attacatgat
      301 acatctaatg tttccagtag ttctggtaaa acaggaagag aacataattg attatttgag
      361 acatgaagct ccttcaaaga tatggggagt gtgggtagtg tgattagttg attgtgggac
      421 gcattcaata atttaagtcc ttgaggcaaa gcaggcagtt caataagtct gttatagctg
      481 acattaagtt gtgtaaggga cgcaggcaat ggggagatta agcttaaatt atttttactt
      541 atattaattg atttaattcc cggggggatt tcaggtaatg ttgtcaggcc taattcagac
      601 aagtccaggt tcgtctcttg gttttgtaga catgatacta gtcgctgaaa agcgatgtct
      661 cgttgttctt cttgtatgcg gttgtttttc cattcagtcc aatgggttag ataattttca
      721 tatgcgcggc tagtgtcgat tgaataagtg gagaacgaat ttgaaattaa tctgtgattg
      781 ttatttgtcg ggagcataaa tatcagggcg tattctttat gttgagaggt gttggattct
      841 tttttatctg ctttgcaatg ttattgattc ccttcagcag tagacaggaa cttctggagg
      901 aggcggatat atagtttgtg taagcatatt aactccatat gttatatatt gaagaacttc
      961 tgctttacac tatcctaaac tggatcggtt taatgtaaaa aaacgatact ttttggggga
     1021 gggggcaatt tttccttaag gtatgaaaaa aggagctgaa gctatataat agctattaat
     1081 gccactggta tcagtgaatc atgtatataa aaaactgtgt taaggaggct gtccattagc
     1141 ggttttttat tcttctggaa ccgcggcatc tttgcgtatc tttttgtctt ctcaccgtct
     1201 ggtcggtgtt ttgctgagac tgctaatttc ctgtctttgc tagtgctgta ctggtactgc
     1261 ccaatcatga ttggcggggg cgggcggagt tgagaccgca gacacgtcgt atgcaagaac
     1321 gtgctgcggc tggctggtga acttccgata gtgcgagtat tgaatgattt ccagccgtta
     1381 ctgattttac gtgctaatta gtgaacaaac cactcgtcag cagactccca ggtatctttc
     1441 agagtttcct gaacaaaagt tttagctgaa tctttatcgg cggtgcgcgt aacagaaagg
     1501 ccatcgttgc tggtggcttt tacgatcacc tctacatcgt cataacgttt actgatgcgt
     1561 cgggttaatt cttcctttaa cgcatccaca gcaccggttg gcattttagt cattttctct
     1621 ttggctatgc agatttcaat acgcataaaa atccctctac actgtgtttt tatacagtat
     1681 tatttttaac tgtatggata aacagtgtca agagtgaggg aaagtgattt tttggggcat
     1741 gcatggggca aaaatatttg ttgtggggca ttttggggca tgattgggac actttgttac
     1801 atatgaactt tgccgatttt catatgacat ggcaatacaa cgctttgatt attctaaaga
     1861 aaacacatgc tcttgggcgt tctttagtta tttttaaaaa tcccgtatca cgcagttaaa
     1921 gtggcgggca tactcttcaa ggctggtgat ccccaaccgc acccatttag gatgcgacca
     1981 ttgcggtaga ccgatgtaaa tcataacgcg tgaaggatct cttccacgct acgcacacgg
     2041 gcgatgcgcg ggtagatatg attaatgctg ttattgtgtt gctcggcgct ggcggcacta
     2101 caggcgtctt cggcaatcac cagattaaag ccaagttccc aggcattgcg ggcggtggat
     2161 tcaacaccga tattggtcga gatcccacat aacactattg tatcgatacc tcggcggcgc
     2221 agttgcaact ccagatccgt accgtaaaac gcaccccact gacgtttgat gatttcgata
     2281 tcactgtcag ttgcacctaa tgcagctgga tgttgccacc agttttcggg caacacttta
     2341 gcggggg
//
LOCUS       DAJVQH010000242         2315 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJVQH010000242 DAJVQH010000000
VERSION     DAJVQH010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.246"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..987)
                     /gene="ipaH7.8"
                     /locus_tag="OQM87_004154"
     CDS             complement(<1..987)
                     /gene="ipaH7.8"
                     /locus_tag="OQM87_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCS1510046.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGPQIYFSMSDGQQNTLHRPLAD
                     AVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQV"
     gene            1316..1495
                     /locus_tag="OQM87_004155"
                     /pseudo
     CDS             1316..1495
                     /locus_tag="OQM87_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1512..2202)
                     /locus_tag="OQM87_004156"
                     /pseudo
     CDS             complement(1512..2202)
                     /locus_tag="OQM87_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          668 a          434 c          535 g          678 t
ORIGIN      
        1 gacctgttca cggaatccgg aggtattgcg tgcagagacg gtatcggaaa ggcggtcaag
       61 gaacgcggaa aaggtgttgg catgctcttc atgttcaaaa gcatgccata tctgtgatac
      121 atcagattgt ttgttttccg ggaaccatgc tgtcacggca tcagccaggg ggcgatggag
      181 tgtattctgt tgtccgtcac tcatggagaa gtaaatctgt gggccgtggt agtccggcga
      241 agaggttaat ctttgcaggg attgcagaac gcgagtcgac aatggattcc ctgaaatatt
      301 aatcctgtaa cttgaatcta attgtgaaaa actctccgga agagtggtta tctgattaaa
      361 atgaaaataa tattcctttt gtctgatatt gtttctatca gaaaattgag gaaaagtaac
      421 cagattattg cgctgagcac agagaatttc cagtttatcc ggtaagtcgg gtaatgttgt
      481 cagcctgtta taataaacat gtaaaaattt caatttttcg ggaagagccg gtaagttata
      541 tagcctgtta ttttcaacaa ataatgatgt taaatggttt ggcaacacag gaaggttttc
      601 cagttgatta gaacgaacac ttaaagttgt taatgactca ggaagttctg gtatcacaga
      661 aagcctgtta ttatcagcat taagttcttt gagaaatgca ggcagctttg gcaatgatgt
      721 taatttatta ttttctacaa tcaaagcaga aatatgcttt ggtaattcgg gaagagacgt
      781 caaatttagg tctgataaat ttaatactgc ttcttgattc tgaaagcact gggagagtct
      841 gttaaaagca atgcctcgct cttccccggg agaagagttc ttttcccatt cagtcaggat
      901 tctcagataa tgttcattac tggttgagtt tgagttaata gagggggagc aagaaactga
      961 tgagtgtgta ttatttacag agaacataat gctaggtcaa ccttatattt gtgagaataa
     1021 atatttaaaa ttgtatattt atacaaattt cacttaccat ttccgatatt aagttttaat
     1081 agtttccatt ttacacataa cagaagttat gttgtaattt atttatggag gttttctatg
     1141 gaattctttt ttattgtgac tgcagtcaat aataacactg aattcaacat tccagtgagc
     1201 aaggaaatgt aatccgagta agtatatttg ttttacatca tattttagtc aagctcattt
     1261 attcctatta tagaatgata aattaaaatt actgtaaaaa aacgatacac atttaatacg
     1321 agggaggccg gcagaaaaac ggcttgcggt aaggcaggag aaatgtgtgc cgcgaatgaa
     1381 gtccttgtat gactggataa agctccagag gaaaactttg tcaaaccatg ctgaaatggt
     1441 aaaggcgttc aactatatcc tgaatcactg gaatgcactg aatgagtttt accgggtagt
     1501 acttccaatc attagaatat gtgcttttca atgaaggaac cgatgacttt ttcgtggatt
     1561 tccacggaac gcgagaagca gattgtttta cgtgccagcc gcttgatgcg ggggcggagg
     1621 gtcaggttgt tacgttcata cgttgcgtga agattttgcc ggtcaggtgt ttctcctccg
     1681 gcacttctct ggcgtagctg ccccagtcat cgctggtcac catgccgata cagaacggag
     1741 tcaacagggc taacagttcc cgacaggtct catcattccg tggcccgcaa gtgtaagcca
     1801 acacaccgcc agttttagtg ttgtacgcat accagggcca gtgttgttgc gccttgctgc
     1861 caacgaagcg ccactgctca tcaagttcac agataagggc aacatcagcg tgagcgactg
     1921 gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc cgtatgacgg tattgatgcc
     1981 gattttcagt gttctggcgg tataacagcc ccctgcgccg ttatgtgcca tttcaacgat
     2041 ctgctcctta acgccgagta ttctggcttc ataagagtaa gtgagctgga aaacacgttt
     2101 acatgagcga caacggaaac gctcatgccg ggaacaacta cggccatgtc gataaacttc
     2161 atcggaatga cagcgcggac aatgaacagt aacggtagcc atggaaaacc ttaaatacca
     2221 gtagattacc agaaacttct gtcgattgaa gacatcaccc gaagtttgaa ttcattcgaa
     2281 tactttttac gaggttcttt tcgccagtcc tgtaa
//
LOCUS       DAJVQH010000243         2303 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJVQH010000243 DAJVQH010000000
VERSION     DAJVQH010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.247"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(164..673)
                     /locus_tag="OQM87_004157"
     CDS             complement(164..673)
                     /locus_tag="OQM87_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510047.1"
                     /translation="MSSKASALFNNNDWKLHVTDINLYENTVSLDGQSYPLSFAIKTL
                     IPGYLSGLPATSRESMELLEALAEAGVTIGNFFSNDLMTAYQRRQQNKRAEAERIAKE
                     QRIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADIE
                     SGGNWMDSL"
     gene            complement(775..996)
                     /locus_tag="OQM87_004158"
     CDS             complement(775..996)
                     /locus_tag="OQM87_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510048.1"
                     /translation="MTTKQHIDPRTPIGKATLRYRGLPTRRLLSMLGMGVEDPERPFY
                     SRDELIDLLVDRDLNNQLRRAFAKLDATH"
     gene            complement(1243..2088)
                     /locus_tag="OQM87_004159"
     CDS             complement(1243..2088)
                     /locus_tag="OQM87_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510049.1"
                     /translation="MNTNEVSVINLDQLVSMSSYDFLTKVINPARVEAGEKPVRNNDF
                     VSRIEDELDGQNVAYEIFVSFGQDVKSYTLNTDQLLLVGMRESKAVRRKVLAYIRQKE
                     QEVLELQAQQLQVAQTKAICYAPGAIVLDSKLDSKSGATPTEVIKALDLKLTSSKMLT
                     ASVLTGYLKTLCRPNPFYTKRVTEFGTTDIDLSTGRPRAIFNKKGETNGYYRLQPQPN
                     DKEPTIKILRKGYDWLKDNWKDIAHDMRTSNIKVVRRLVDSHLNGKATKSQKAAAGKG
                     SDYGF"
BASE COUNT          526 a          559 c          610 g          608 t
ORIGIN      
        1 ggcagacctt cgttaacctg aatgttgtcg gtgtcggtga tacgtactgg ctcaatgccg
       61 aactgatagc tatctttaat catggtcata ataatcaatc tcctaaatta gttttagata
      121 ctgtgggtcc cgacatcagg tcaggaccac ttgtgtgtct gtcttagagg ctgtccatcc
      181 agttaccgcc tgactcaatg tcagcgacaa tagcggcacg ggagcgacca gcggaatggg
      241 tagcactgcg gaggttctca ccataggtcc ggcgctcagc tttgacctgt tcgcggcgct
      301 gtagctcttt ctgccactct gcatcggtca tgttctcttc acgcatacgc tcagcctgaa
      361 tgcgctgctc tttagcgata cgctcagcct cggctcgctt gttctgctgg cgacgttggt
      421 aggcggtcat caggtcattg ctgaagaagt ttccaatggt cacgccagct tcagccagag
      481 cttccagtaa ttccatggac tcgcggctgg tggcaggcag tcctgacagg tagcccggaa
      541 tcagggtctt aatagcgaag gacagtgggt aggactgacc gtccagactc acagtgtttt
      601 cgtagagatt gatgtcggtg acatggagtt tccagtcatt gttgttgaat aacgcactag
      661 ctttgctaga catagtaatt actcctcttg agagttgaga ctatcggtac cgcagacaca
      721 gttatgccta cgggtctccc cgcatcggtc atctcaaaag gtttaaatta ggggttagtg
      781 agtggcgtcc agcttagcga acgctcggcg taactgattg ttcaggtcac ggtcgaccag
      841 caggtctatc agttcgtctc gactatagaa aggacgctca gggtcctcta cgcccatccc
      901 cagcattgac aggaggcgac gggtaggcag accacggtaa cggagagtag cctttccgat
      961 gggtgtccgt gggtcgatat gttgtttagt tgtcattgtt agaagtcctc gttagaccaa
     1021 tcatccacgt caaattcact ggtgatagga gcttggaagt taggtagacc ctctggtatc
     1081 tctagaggca gtcgctcgtc gactctcagt gccatctctt cgtgtatatg ccgaagcaga
     1141 gaagtcagta agatagggtc cgcaggatgc tcagggccgt gtctctcaat gaaacatatg
     1201 gttgtcgcta ttgactccat aatcagttca tcagtgagtc gcttagaacc catagtcaga
     1261 ccccttgccc gctgctgctt tctgggactt tgtagcctta ccattcaggt gagaatccac
     1321 cagacgacgg acaaccttga tattggatgt gcgcatgtca tgagcgatat ccttccagtt
     1381 atccttcagc cagtcataac ccttgcgtag aatcttgatg gtaggctctt tgtcgttagg
     1441 ctgtggctgt agacgatagt agccgttggt ctcacctttc ttattgaaga tggctcgtgg
     1501 acgtccggtc gataggtcta tatccgtagt gccaaactca gtaacacgct tggtgtagaa
     1561 tgggttagga cgacacaggg tcttcagata tccagtcagg acgctagctg tcagcatctt
     1621 agagctggtc aacttaaggt ccagagcttt aataacctcg gtaggcgtgg cgcctgactt
     1681 ggagtccagc ttggagtcga ggacgatagc accgggcgca tagcagatag ctttggtctg
     1741 tgcgacctga agttgctggg cctgaagttc gaggacctct tgctccttct ggcgaatata
     1801 ggctaggacc ttacgacgca cagctttaga ctcacgcatc ccgaccagca gaagctggtc
     1861 agtgttgagg gtatagcttt taacgtcctg cccgaagctt acgaaaattt cgtaggctac
     1921 attttgaccg tccagctcat cttcaatacg ggacacaaag tcgttgttac ggacaggctt
     1981 ctccccagcc tccacacgag ccgggttgat gaccttagtc aagaaatcgt agctactcat
     2041 agacacaagc tggtcaaggt tgatgacgct aacttcgttg gtattcataa aggatgctcc
     2101 tttggtaaag cactttctgt ctccatgtgg agtccgcacc tattcaatgc atccaaacgg
     2161 acccaccaaa aagtgcttaa cgaaagtgaa tagggttaat gttgattagt gttggtctgg
     2221 agtgaccatg ttctccatgt gcatataagg cgagaccgca tgtcaggacg agctgtaagg
     2281 accccagatg gtgagcggtg ata
//
LOCUS       DAJVQH010000244         2295 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJVQH010000244 DAJVQH010000000
VERSION     DAJVQH010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.248"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(154..348)
                     /locus_tag="OQM87_004160"
                     /pseudo
     CDS             complement(154..348)
                     /locus_tag="OQM87_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1466..1978)
                     /gene="clbS"
                     /locus_tag="OQM87_004161"
     CDS             complement(1466..1978)
                     /gene="clbS"
                     /locus_tag="OQM87_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin self-protection protein ClbS"
                     /protein_id="HCS1510050.1"
                     /translation="MAVPSSKEELIKAINSNFSLLNKKLESITPQLAFEPLLEGHAKG
                     TTISVANLVSYLIGWGELVLHWHDQEAKGKTIIFPEEGFKWNELGRLAQKFYRDYEDI
                     TEYEVLLARLKENKQQLVALIERFSNDKLYGKPWYNKWTRGRMIQFNTASPYKNASGR
                     LNKLQKCLAE"
     gene            complement(2013..>2295)
                     /gene="clbQ"
                     /locus_tag="OQM87_004162"
     CDS             complement(2013..>2295)
                     /gene="clbQ"
                     /locus_tag="OQM87_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colibactin biosynthesis thioesterase ClbQ"
                     /protein_id="HCS1510051.1"
                     /translation="MTIFTPIIKNDYRLYEQYVFQAKARTLTCPIVLFHGDADNLVMQ
                     DELLAWEKFTTRKTRTIIFPAADHFFVDKHFEQVVGYVNQTIESLEIVG"
BASE COUNT          645 a          538 c          466 g          646 t
ORIGIN      
        1 gtgacggaac tccaccttgc aggaactggc actgactctg gtctgagtgg aagtggataa
       61 agacggcgca atggccgcca caggttcttt ctgctcaccc ggcgttattt ctacaggtaa
      121 taattcaacg ccagtgtcag aagaggtcgt taccggaaga cgccgcgaaa cacgcccttc
      181 gttctgccag agcctgagcc atttgaaaat aacattatca ttgacgccat tttcacgtgc
      241 aatctgtgca acacaagctc caggttgtga tgccagttcc accatacgaa gtttgaattc
      301 attcgaatag tttttacgag gttcttttcg ccagtcctgt aattccatac ttagatgtcc
      361 gtctatatca gatgggcgtc taagttacca attctcgtct gatggctaca tacggcggtc
      421 agtttacgct tactacaatg atgataatgc gattatataa aggtcaaacc atatggttgt
      481 tagcggattt caacaacggt gctgtactcg ctgagcggca catgaagttt gaataatgtg
      541 gaaacaacaa atggctacat taagtcattg gacatataca gagatggttg gcaaatcgag
      601 tacacgatat gttgcctgaa aagtcgaccg aacagaacta taaatatcaa gtcggcataa
      661 cacatctaaa tttcataagg gaagatgtgc caccggggac gtttttttca ccacggggac
      721 ttcgggtata acgatgcaat ccgaagaaag aaatccaatg ttggcgatat tcatcatcgc
      781 tttattcgtc cactcatgcg aatggcaata aagcgactca ccaaaacagt ttctttagct
      841 tccctctacc aaaattccct atgatcgcat gttagaactc tgcgtgctga acttcgggcc
      901 atatggacgg tatcattcat gcacttgctc ctttgaaatt gctggatatc attcgtcttt
      961 atctgcaccg gccggcacaa atagctgtag gggaacccgg cgcaggcaaa aagagcgact
     1021 ttaacgcgtt ttgcaggact ccccgttgcc ttgcagggag acactgtatc atcaaacacc
     1081 atcttaatcc cagtacatta ccctccatcc tggctcgtac caaatcagag gaaaggcctc
     1141 gcggcaatag attttcccgg cgaagctcgg agtgaaatac agtgcagcaa tatccgacat
     1201 cagaatttga tcctcctgcg tcaggatacg aaaaaacatc ccgatttgag cacccttgtc
     1261 gaaaaaaaat caccttatta tgaagattgg ttcttaatac acatgcgttc gcatgtgctt
     1321 atgttaagta gacttattgt cttacacatt ttatccgtat cattaaccag aatatgttcc
     1381 ggagcagcgg gtgatagtgc cgccagtcca cactcatcgc tgcccggaaa aatcatcagt
     1441 ggggaggcaa acggtaagca ccccgctatt ctgcaagaca tttctgcagt ttatttaacc
     1501 tccccgaagc atttttataa ggcgaggcgg tattaaattg aatcatacga cctcgggtcc
     1561 atttattata ccaaggttta ccgtaaagct tgtcgttact gaatcgttca atcaaagcca
     1621 cgagttgctg cttattttcc tttaaccgtg ccaataaaac ttcgtactct gtaatatcct
     1681 catagtcacg gtagaatttc tgtgctaaac gccccaattc attccattta aatccttcct
     1741 caggaaaaat aatagttttt ccttttgcct cttggtcatg ccagtgtaac accagctctc
     1801 cccagccaat cagataggaa accagattcg ctacgctaat cgtagtcccc ttcgcgtgcc
     1861 cttccaatag aggttcaaag gcgagttggg gcgtaataga ttctagcttc ttatttaata
     1921 aagaaaaatt actattaata gctttaatta actcttcttt tgatgatgga acagccatat
     1981 ctattgctcc ttgtatagtt acacaactat ttctacccta ctatttcgag tgattcaatc
     2041 gtctggttca cataacctac cacctgttca aaatgcttat cgacaaaaaa atgatcggca
     2101 gcaggaaata taatagtccg cgtctttcgt gtggtgaatt tttcccatgc aagtaattca
     2161 tcctgcatta ccagattgtc agcatcgcca tgaaatagca cgatcggaca ggttaatgtg
     2221 cgcgccttgg cctgaaatac atactgctca tagagccgat aatcgttttt aatgatgggg
     2281 gtgaaaattg tcatt
//
LOCUS       DAJVQH010000245         2295 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJVQH010000245 DAJVQH010000000
VERSION     DAJVQH010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.249"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            21..1073
                     /locus_tag="OQM87_004163"
     CDS             21..1073
                     /locus_tag="OQM87_004163"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCS1510052.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1149..2295
                     /locus_tag="OQM87_004164"
                     /pseudo
     CDS             1149..2295
                     /locus_tag="OQM87_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
BASE COUNT          557 a          510 c          599 g          629 t
ORIGIN      
        1 tttaaattga gggcattatt atgaaaaaaa taccctgcgt gatgatgcga ggtggaacct
       61 cgaggggcgc gttcctgtta gcggaacatt tacccgaaga tcaaacgcag cgcgataaaa
      121 tattgatggc aattatgggt tccggtaacg atctggaaat tgacggtatt ggcggcggta
      181 atccactgac cagtaaagtc gccattatta gccgttccag cgatccgcgt gctgatgtcg
      241 attatctgtt tgctcaagtt atcgtccatg agcaacgtgt cgataccacg cctaactgcg
      301 gcaatatgct gtctggcgta ggggcattcg ccattgaaaa tggtttgatt gcagcgactt
      361 cgccagttac ccgtgtacgt atccgcaacg tcaatacggg tacgttcatc gaagctgatg
      421 tgcaaacgcc aaatggtgtt gtcgagtacg agggtagcgc cagaattgac ggcgtaccgg
      481 gtactgccgc accggttgcg ctcactttcc tgaatgccgc tggaacgaaa accggaaaag
      541 ttttcccgac tgataatcag attgattatt ttgacgatgt cccggtgacc tgtatcgata
      601 tggcgatgcc agtcgtcatt attccggctg aatatctggg aaaaacaggt tatgaattac
      661 cggcggaact ggatgccgac aaagcattat tagcccgcat tgaatctatc cggctacaag
      721 cgggtaaagc aatgggctta ggtgatgtca gtaatatggt tatccctaaa cctgtgctta
      781 tttctccagc gcagaaaggc ggggcaatta atgtgcgtta ttttatgccg cattcttgcc
      841 atcgcgcgct ggcgataacc ggtgctattg ctatttccag tagttgtgca ttggaaggca
      901 ccgtcacccg acaaatcgtc ccttctgtag gatatggcaa tatcaatatt gaacacccca
      961 gtggtgcgct cgacgttcat ttaagtaatg aaggtcagga tgccacgacg ttacgcgcat
     1021 ctgttattcg gacaaccaga aaaatatttt ccggtgaagt ttatcttccc tgaaaaaatt
     1081 cgttgtcagg ataaggacaa tcaataaagg acttctgtat gagtcataca gaaagaacag
     1141 gattttaaat gaataagaaa tcgttatgga agctaattct gatattagcg atcccatgta
     1201 ttattggttt tatgccagct ccggcaggat taagcgaact ggcgtgggtg ctttttggta
     1261 tttacctggc ggccattgtg ggtctggtta tcaagccttt cccggaacct gtcgtactgt
     1321 taattgccgt tgctgcctca atggtggtgg tcggtaactt atccgacggt gcgtttaaga
     1381 ccaccgccgt attaagcggt tactcttcag gtactacctg gctggtgttc tcggcgttta
     1441 ccttaagcgc cgcatttgtg accaccggtt taggtaaacg tattgcctat ctgctgattg
     1501 gtaaaatcgg taacaccatg ctgggtctgg gttacgttac ggtattcctc gatctggtac
     1561 tggctccggc aacaccgtct aacaccgcgc gtgcgggcgg cattgtgtta ccgatcatca
     1621 acagcgtggc ggtggctttg gggtccgaac cggaaaaaag tccgcgtcgt gtcggacatt
     1681 acctgatgat gtccatttac atggtcacca aaaccaccag ctatatgttc tttaccgcaa
     1741 tggcggggaa cattctggcg ctgaaaatga tcaacgacat tctgcacctg caaattagct
     1801 ggggtggatg ggcgctggcc gccggattgc cggattgccg gattgccgga ttgccggatt
     1861 gccggattgc cgggcatcat tatgctgctg gtcaccccgc tggtaattta caccatgtat
     1921 ccaccagaaa ttaaaaaggt ggataacaaa accatctcta aagcgggcct tgccgaactg
     1981 ggaccgatga aaatccgcga aaaaatgctg ctcggtgtct ttgtgctggc gctactgggc
     2041 tggattttca gtaagtctct gggggttgat gaatccactg tggcaatcgt tgttatggcg
     2101 accatgctgc tgctgggtat cgttacctgg gaagacgtgg ttaaaaataa aggcggctgg
     2161 aataccttaa tctggtacgg cggtattatc ggcttaagct ccttattatc gaaagttaaa
     2221 tttttcgaat ggttagctga agtctttaaa aataacctgg catttgatgg tcacggtaac
     2281 gttgctttct tcgtt
//
LOCUS       DAJVQH010000246         2241 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJVQH010000246 DAJVQH010000000
VERSION     DAJVQH010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.250"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(155..667)
                     /locus_tag="OQM87_004165"
     CDS             complement(155..667)
                     /locus_tag="OQM87_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCS1510053.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            862..1626
                     /locus_tag="OQM87_004166"
                     /pseudo
     CDS             862..1626
                     /locus_tag="OQM87_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1577..>2241
                     /locus_tag="OQM87_004167"
     CDS             1577..>2241
                     /locus_tag="OQM87_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCS1510054.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          641 a          488 c          566 g          546 t
ORIGIN      
        1 atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg
       61 gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg
      121 gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc
      181 taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca
      241 ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatgtaa cgcagcacgg
      301 cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc
      361 gttctattgt attggaacgg ctcgatatta tacatgcaag ttgagggata tgcccaatta
      421 ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc
      481 cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg
      541 atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa
      601 gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact
      661 ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat
      721 ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt
      781 cgcaggagtg agtgatgagg aagaattaaa gcaacctatt gtgacaggca acgataacag
      841 taaatcacag gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac
      901 cgggccggag tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct
      961 atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa
     1021 aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca
     1081 caaaaaagta cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg
     1141 cgtaccgatc ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt
     1201 ggttatgccc gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg
     1261 ctcgaggacg aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt
     1321 cggtggttgg tgtcgctgga agatgctaat ggggactagg aaaggaagat gctgattgaa
     1381 aatatcagcg ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt
     1441 ggcacgcttg cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact
     1501 gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat
     1561 ggagagcgtg acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt
     1621 acgtagtttc tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc
     1681 aaaatcatgg gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt
     1741 ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat
     1801 tcagatagac aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa
     1861 ccgggttact ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa
     1921 gtcactggag gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca
     1981 gtggaaaatt ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa
     2041 ccccggactt gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac
     2101 tctcatccgc aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt
     2161 tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc
     2221 ggagtctgat gatgatgcgg c
//
LOCUS       DAJVQH010000247         2128 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJVQH010000247 DAJVQH010000000
VERSION     DAJVQH010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.251"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..234
                     /locus_tag="OQM87_004168"
                     /pseudo
     CDS             1..234
                     /locus_tag="OQM87_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            655..1233
                     /locus_tag="OQM87_004169"
     CDS             655..1233
                     /locus_tag="OQM87_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510055.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1342..1600)
                     /locus_tag="OQM87_004170"
                     /pseudo
     CDS             complement(1342..1600)
                     /locus_tag="OQM87_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1584..2128
                     /locus_tag="OQM87_004171"
                     /pseudo
     CDS             1584..2128
                     /locus_tag="OQM87_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          688 a          390 c          523 g          527 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agatcggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggagggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctacggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagaca atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatgg catattacca tgcaaatggt agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctactggca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa agaacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttgtagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataac
     1501 atgactggtt gaggacaata aagctcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg
     1621 agaaaatagc cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa
     1681 cccgccagaa tgaacatccg catcttccta ttgaaagtcg tcgtcagaca aagggaactc
     1741 gcttcctgtg gcagcacagc gataaatgga tgaccgaatc caggcaggaa aagctgatat
     1801 ggctgcgtgc gcagatgaag ctgacgagcc tgtgctgggc gctgaaagaa ctggcaaagg
     1861 atatctggag cagaccatgg agcgaggaaa gacgcaacga ctggcagaga tggctgcggc
     1921 caacagtgac gtccccatga tgaagaatgt ggctaaaacg atagggaaaa ggttgtacgg
     1981 tatcctgaac gccatgcgcc atggcgtatc aaacggaaat gcggaagcac tgaacagcaa
     2041 gatcaggctg ctgagaataa aagccaaggg ctaccgcaac cgggagcgct ttaagctggg
     2101 agtgatgttc cactatggga aactgaat
//
LOCUS       DAJVQH010000248         2128 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJVQH010000248 DAJVQH010000000
VERSION     DAJVQH010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.252"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            529..2037
                     /gene="ltrA"
                     /locus_tag="OQM87_004172"
     CDS             529..2037
                     /gene="ltrA"
                     /locus_tag="OQM87_004172"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCS1510056.1"
                     /translation="MQRKLATWAVTDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          540 a          526 c          622 g          440 t
ORIGIN      
        1 atgcccgcag ggtgatgtaa cccgctgaca acggggattg aggcgagatc actaagccga
       61 gatgatcctc aaggttaagt actgaaaggc tgaagaacat gaacccgtta atccgcctct
      121 gtgggttgaa aacgtcacca cggcctacgt gatctgacag gccgtgcagg aggaactggc
      181 agtgatacgt aagcactgcc ggtcgaaggt gttttgacat gtatgcgaaa caccggggca
      241 gcagcgtcta tcacgcttgc gttgctgact tctgccaact tgcggcaagc aaggataaag
      301 agtgcgacgg gcagcctcct cagtatgcct gagtccaggc aggtaaaccg gggaaggtca
      361 gcgacggatg ttaagggggc atggctccga tgacgcgctg gctggcggag cttccgtagt
      421 agtccgcgat ggggaaagcc cattacatgg cgaagggaag cagtttgaat gtgtttgcga
      481 cgtgaattaa ctgacctaac gaggtgaaga cctttgataa tcagcgaaat gcaacgcaag
      541 cttgccacat gggcagtcac cgatccgtcc ctacggattc aacggctgct gcgtctgata
      601 acacaaccag aatggctggc tgaagcggcg cggatcacgc tttcatcaaa gggggcccat
      661 acccccggcg ttgatggcgt gaacaaaaca atgctacagg ccagactggc tgttgagctg
      721 caaatcctca gggatgaatt actctcaggc cactaccagc ccttgcccgc cagacgggtt
      781 tacatcccta aaagcaacgg caaactgcga ccactgggta tccccgcgtt gcgggatcgt
      841 attgttcagc gggccatgct gatggcgatg gagccgatat gggagagtga ttttcatacg
      901 ctctcatatg gcttccggcc tgagcgcagt gtccaccacg ctatccgcac ggtgaaatta
      961 cagctcacag actgtggtga aacccgggga cgctgggtga ttgaaggcga cctgtccagt
     1021 tacttcgaca ccgtacatca tcgactgctg atgaaggctg tacgccgcag gatcagtgac
     1081 gcacgtttca tgactctgct gtggaaaacc atcaaggcgg gacatatcga tgtcggtctc
     1141 tttcgggcgg ccagtgaagg tgtgccacag ggcggtgtta tatcgccgct attatcgaac
     1201 atcatgctga atgagttcga tcaatacctg catgagcgct acctgagcgg gaaagccaga
     1261 aaagatcggt ggtactggaa taacagtatc caacggggcc gaagtacggc ggtcagagaa
     1321 aactggcagt ggaaacccgc ggtggcgtac tgccgctatg ccgatgattt tgtcctcatc
     1381 gtcaaaggca ccaaagcaca ggcggaagcc atcagggagg agtgtcgggg tgtgctcgaa
     1441 ggcagtctga aactcaggct gaacatggat aagactaaaa tcacccatgt taatgacggc
     1501 tttatctttc tggggcacag gatcattcgc aaacgcagtc gttatggcga gatgcgagtg
     1561 gtctcaacga tcccgcagga gaaagccaga aacttcgccg catcgctgac agcactgtta
     1621 tcaggcaact acagtgaaag caaagtcgat atggctgaac aactcaaccg aaaactgaaa
     1681 ggctgggcca tgttctatca gttcgttgat tttaaggcca aagtcttcag ttatatcgac
     1741 cgtgtcgtgt tctggaagct ggctcactgg ctggcccgca aataccgtac aggtatcgct
     1801 tccctgatga ggtggtggtg taaatcaccg aaaccgggtc agagcaaaac gtgggtttta
     1861 tttggtaaaa ccaatcacgg caagctcagc ggcgaaatac tgtaccggtt ggtggggcaa
     1921 ggcaagaagc tgttccgctg gcggctaccc gaaggtaatc cctatctgag gacggagacc
     1981 agaaacacgt atacatcgcg ctttacagaa gtggcaatgg cgttcgccag catttaaatg
     2041 gagagccgga tgcgctgaaa ggtgcacgtc cggttcgggg aggagaggca gggaaatagt
     2101 ccgactacgc cctgcctctt actctact
//
LOCUS       DAJVQH010000249         2116 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJVQH010000249 DAJVQH010000000
VERSION     DAJVQH010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.253"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..243
                     /gene="etp"
                     /locus_tag="OQM87_004173"
                     /gene_synonym="yccY"
     CDS             <1..243
                     /gene="etp"
                     /locus_tag="OQM87_004173"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCS1510057.1"
                     /translation="GRKLTAEMARNYDLILAMESEHIAQVTAIAPEVRGKTMLFGQWL
                     EQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            263..>2116
                     /gene="etk"
                     /locus_tag="OQM87_004174"
     CDS             263..>2116
                     /gene="etk"
                     /locus_tag="OQM87_004174"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCS1510058.1"
                     /translation="MTTKNMNTPLGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMLPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINTLQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQHQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVP"
BASE COUNT          569 a          501 c          557 g          489 t
ORIGIN      
        1 ggacgtaagc tcactgctga gatggcacga aattacgatc tgattctggc aatggagtcg
       61 gaacatattg ctcaggttac cgcaattgct cccgaggtcc gcgggaagac aatgctgttt
      121 gggcaatggc tggaacagaa agagatcccg gatccctatc gtaaaagtca ggacgcattt
      181 gaacatgtct acggtatgtt ggagcgcgcc agtcaggaat gggcgaagcg cctcagccgg
      241 taatttgagt ttataaaata cgatgacaac taaaaatatg aatacgccac taggcagcac
      301 tcaggaaaat gagatcgatc tgcttcgtct ggtcggcgag ttatgggatc accgtaagtt
      361 tattatcagc gtgaccgcgt tattcacgct gatcgctgtc gcttactcgc tgttaagcac
      421 accaatttat caggcagata ctctggtcca ggttgagcaa aaacagggca acgccattct
      481 cagcggcctg agcgatatgc tccctaactc atcgcccgag tctgcaccgg agatccaact
      541 gctgcaatcg cgcatgattc tcggtaaaac cattgctgaa ctgaatctgc gcgacatagt
      601 tgagcagaag tattttccga ttgtgggtcg cggctgggcg agattaacca aagaaaaacc
      661 aggtgagctg gcgatcagct ggatgcatat tccacaactg aatggtcagg atcagcaact
      721 gacactcacg gttggggaaa acggccacta tacactggaa ggtgaagagt tcaccgtcaa
      781 tggtatggtc ggccagcgtc tggaaaaaga tggcgttgcg ctgactatcg cggacattaa
      841 ggccaaacca ggaacacagt ttgtcctgag ccagcgtacc gaactggaag cgattaacac
      901 attgcaggaa acctttaccg ttagcgaacg cagtaaagaa agcgggatgc tggaacttac
      961 catgactggt gatgatcccc agttgattac tcgtattctg aacagcatcg ctaacaacta
     1021 tttgcaacag aatatcgctc gccaggcggc gcaggattca caaagccttg aattcttaca
     1081 gcaccagtta cctgaagtgc gcagcgagct ggaccaggcg gaagaaaaac tcaacgttta
     1141 tcgccagcag cgcgattcgg ttgaccttaa cctggaagcc aaagccgttc ttgagcagat
     1201 tgtgaacgtt gataatcaac tcaatgagct gaccttccgc gaggcagaga tctcccagct
     1261 gtataagaaa gatcacccaa cttatcgtgc gctgctggaa aaacgccaga cgctggagca
     1321 agaacgcaaa cgcctgaata agcgggtatc ggcaatgcct tccacccaac aggaagtgtt
     1381 gcgtttaagt cgtgacgtag aagcgggccg tgcggtatat ctgcaattac ttaaccgcca
     1441 gcaggagttg agtatttcga aatccagtgc cattggtaac gtgcggatta tcgacccggc
     1501 agtcactcag ccgcagccag tgaaaccgaa aaaagcgttg aatgtggtgc ttggttttat
     1561 tcttggcctg tttatttcgg tgagtgccgt gctggcgcgt gcgatgttgc gtcgtggtgt
     1621 agaagccccg gaacaactgg aagagcacgg catcagcgtt tattccacta tcccaatgtc
     1681 cgagtggctg gataaacgca cccgtctgcg taagaaaaat ttattttcta atcagcagcg
     1741 ccatcgtact aaaaatatcc ccttcctggc ggtggataac ccggcggatt ctgctgtgga
     1801 agccgtacgt gcgctacgaa ccagtctgca tttcgctatg atggagactg agaataacat
     1861 tctgatgatc accggtgcga cgccagacag tggtaaaacg tttgtcagtt caactctggc
     1921 agcggtgatc gcccagtccg atcaaaaagt gttatttatt gatgccgact tacgccgtgg
     1981 ttattcgcat aacctgttta ccgtgagtaa tgaacatggc ttgtcggaat atctggcagg
     2041 taaagatgag ctcaacaaag tgatccagca ttttggcaaa ggaggctttg atgtgattac
     2101 tcgcggtcag gtgcca
//
LOCUS       DAJVQH010000250         2107 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJVQH010000250 DAJVQH010000000
VERSION     DAJVQH010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.254"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..246)
                     /locus_tag="OQM87_004175"
     CDS             complement(<1..246)
                     /locus_tag="OQM87_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510059.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFC"
     gene            271..510
                     /gene="relB"
                     /locus_tag="OQM87_004176"
     CDS             271..510
                     /gene="relB"
                     /locus_tag="OQM87_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCS1510060.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            510..797
                     /gene="relE"
                     /locus_tag="OQM87_004177"
     CDS             510..797
                     /gene="relE"
                     /locus_tag="OQM87_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCS1510061.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            869..1024
                     /gene="hokD"
                     /locus_tag="OQM87_004178"
     CDS             869..1024
                     /gene="hokD"
                     /locus_tag="OQM87_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1510062.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1242..1492
                     /locus_tag="OQM87_004179"
                     /pseudo
     CDS             1242..1492
                     /locus_tag="OQM87_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1559..1837
                     /locus_tag="OQM87_004180"
     CDS             1559..1837
                     /locus_tag="OQM87_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510063.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            1839..>2107
                     /locus_tag="OQM87_004181"
     CDS             1839..>2107
                     /locus_tag="OQM87_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510064.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRH"
BASE COUNT          544 a          481 c          550 g          532 t
ORIGIN      
        1 gcaaaaaata gatgaataag ttgagttgtg catatgtagc ctgaccgtca caaagtatat
       61 ggtgtctgta ccagtaagat gatggccgga ctctttaaaa aagagctgac ctgcacaata
      121 caggatggac ttagcaatgg ctgctcctgg cacaaagcgg acagtgatca ccgttcttac
      181 gactactttc tgacttcctt cgtgacttgc cctaagcatg ttgtagtgcg atacttgtaa
      241 tgacatttgt aattacaaga ggtgtaagac atgggtagca ttaacctgcg tattgacgat
      301 gaacttaaag cgcgttctta cgccgcgctt gaaaaaatgg gtgtaactcc ttctgaagcg
      361 cttcgtctca tgctcgagta tatcgctgac aatgaacgct tgccgttcaa acagacactc
      421 ctgagtgatg aagatgctga acttgtggag atagtgaaag aacggcttcg taatcctaag
      481 ccagtacgtg tgacgctgga tgaactctga tggcgtattt tctggatttt gacgagcggg
      541 cactaaagga atggcgaaag ctgggctcga cggtacgtga acagttgaaa aagaagctgg
      601 ttgaagtact tgagtcaccc cggattgaag caaacaagct ccgtggtatg cctgattgtt
      661 acaagattaa gctccggtct tcaggctatc gccttgtata ccaggttata gacgagaaag
      721 ttgtcgtttt cgtgatttct gttgggaaaa gagaacgctc ggaagtatat agcgaggcgg
      781 tcaaacgcat tctctgaacc aaagcatgac atctctgttt cgcaccgaag gtgacacttc
      841 tgctttgcgt tgacaggaga agcaggctat gaagcagcaa aaggcgatgt taatcgccct
      901 gatcgtcatc tgtttaaccg tcatagtgac ggcactggta acgaggaaag acctctgcga
      961 ggtacgaatc cgaaccggcc agacggaggt cgctgtcttc acagcttacg aacctgagga
     1021 gtaagagacc cggcgagtga gaaatccctc gccacctctg atgtggcagg catcctcaac
     1081 gcacccgcac ttaacccgct tcggcgggtt tttgttttta ttttcaacgc atttgaagtt
     1141 ctggatggtg ccggaataga atcaaaaata cttaagtagc gcgcagggat aaaagggatg
     1201 gaccccgaac aggggagtgc tacttatctg gaaggattct gatgatgaac atcgaagaac
     1261 tgcgtaaaat tttttgtgaa gatggcctct atgctgtgtg cgttgaaaat ggaaatattg
     1321 ttagtcatta ccgcattgtg tgtttgcaaa aaatggggct gcgttaatta attttgtgga
     1381 tgcccgagtg acggacggat ttatcttgcg cgacggtgag tttgtcactt cattacaggc
     1441 attgaaagag atcggaataa aagctggctt ttctgctttt tcagaagaat aaactcatct
     1501 acaatcttgc gcggggctga actcccgctg agtaacaccg tgccaccgga gaaaaccgat
     1561 ggcacgcaac gcaaaatatt acaatcatga taattcgacc gttcttgccc acacgcacga
     1621 gcggtattct cacgcattta agtcagactg gtaccagcat ccctcatgca ctgaagaaca
     1681 ggccgaatgg ctcattcagt gttaccgcag gcgcggatgc gaggttaaaa aagcccttag
     1741 cctcgactac cgtcactgga taatctccgt caggctccct tactccgaac agccaccgcg
     1801 tccgtcccgc acattccagc aacggatctg gaggtaacgt gcgggtatta cttcgacctg
     1861 ttctggttcc ggaactcggg ctggtggtcc ttaagcccgg tcgtgaatcc atgccggtat
     1921 tccacaatac ccggttactg gtggagccgg aaccgaaaag catgcgtaat ctgccgtccg
     1981 gggtcgttcc tgccgctcgc cagccgctgg tggaagacaa aacattgctg ccgtttttca
     2041 gtaacgcacg ggtgattcgt gctgctggtg gtgctggtgc attgtctgac tggctgttgc
     2101 gccatat
//
LOCUS       DAJVQH010000251         2101 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJVQH010000251 DAJVQH010000000
VERSION     DAJVQH010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.255"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(60..1025)
                     /locus_tag="OQM87_004182"
     CDS             complement(60..1025)
                     /locus_tag="OQM87_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCS1510065.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1891..>2101)
                     /locus_tag="OQM87_004183"
     CDS             complement(1891..>2101)
                     /locus_tag="OQM87_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510066.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          581 a          551 c          440 g          529 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gctgaggata atgcaggata gacagagcac tagagattgc atgcaatctc gtacctatca
     1861 cctcagcctg ccgctggctc ttttccagaa ctaagaatgc gttcttaaaa atccaaactt
     1921 gataccgttt aaatctattt tcacaaaaag agtttttacc ctctcgtttg ttttctgaat
     1981 atataaaatt aattcatcct caaaaccttc gtgcggatga aaaccaaatg tgccaaaact
     2041 taatccatca ataagcaaaa taaggttttt aaacctgtca ggaatcaccg cttcacttct
     2101 t
//
LOCUS       DAJVQH010000252         2086 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJVQH010000252 DAJVQH010000000
VERSION     DAJVQH010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.256"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            7..372
                     /locus_tag="OQM87_004184"
     CDS             7..372
                     /locus_tag="OQM87_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCS1510067.1"
                     /translation="MNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKALKVRQR
                     LTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVAAYVAV
                     NTNKWFYEKEVKGLNTWSL"
     gene            complement(413..1363)
                     /gene="corA"
                     /locus_tag="OQM87_004185"
     CDS             complement(413..1363)
                     /gene="corA"
                     /locus_tag="OQM87_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCS1510068.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            1733..2071
                     /locus_tag="OQM87_004186"
     CDS             1733..2071
                     /locus_tag="OQM87_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510069.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGDIYDESYAPLG
                     LYIENGQRKV"
BASE COUNT          563 a          496 c          505 g          522 t
ORIGIN      
        1 gaatatatga acgatgattc attaagtgaa aaatggaaat accgatttaa tttttatgat
       61 caacatggct ttcctggatt ctggggagca acaccagaat ataaagcagc atttaaagca
      121 ttaaaagtaa gacaacgatt aactattcaa atgaatttca ttgctttctt ctgttcctgg
      181 atctatttat ttgttctggg cttatggaaa aaagcaatta tagttctatt acttggcatt
      241 ctatctctat ttgttggtgc attaattggc gttaacattc tgggaattgc cgtagcagcc
      301 tatgttgctg ttaacacgaa caaatggttt tatgagaaag aggtaaaagg gttaaatacc
      361 tggagccttt gaataacagg tggcttagcc aggctaagcc accgccttct ttttacaacc
      421 agttcttccg cttaaagtac agatacggtg ccaggcccgc gaggatcata aagataatcg
      481 cgccagggta gccgaaactc cacttcagtt ctggcataaa ctcaaagttc atcccgtagc
      541 tggaagcaac cagcgttggc ggcaggaaca cgacggatac caccgagaaa attttgatga
      601 tgcggttctg ctcgatgttg ataaaaccca ttgccgcctg catcaggaag ttcaccttct
      661 ggaacaggga ttcgttatgc ggcagcaggg attcgatatc gcgcaggatt tcacgcgcct
      721 gctccagttg cccacccggt aaacgcgctt tacgcaccag gaagttgaga gcgcgctggg
      781 tatccatcag acacaggcga actttccagc cgatatcttc cagttccgcc agcgtggaga
      841 gcgcctcgtc gtactcatcg ccctgatgcc cttccataat cacccggctc aactgctcca
      901 ggtcgctata gatattttca atttcatctg ccaactgttc gattttggtt tcgaacagat
      961 ccagcagcaa ctcgtaggcg ttaccgtcta ccatcgactg gctacgggca cgcatacgat
     1021 acagacgaaa agcgggcagt tcacgctcac gcagagtaaa cagacgacca tcacggatgg
     1081 taaatgccac agtggagtta ccggcgtgat cttccgcatc ttcaaagaag aagaaggagt
     1141 gaatatgcag gccgtcgtcg tcttcaaaga aacgtgccga tgcttcgatg tcttccagtt
     1201 ccgggcgggt tgccaggctc tggccaagtt cagattgtac gcgcagtcgc tcgtcgtcgt
     1261 ccggttcgac aagatcaatc catactgcat ttacaagggg ttgtgactct tcgacttcca
     1321 gccgggtcag tcggttattt tccagttgaa atgcgctcag catgaccggg actcccaatg
     1381 cttaaaatat cggacagttc ggtgggcaca cagaaacaaa ttgggtttca gaccattaaa
     1441 cagcctgact cagcgcgacg ggaaaaattg aggtcgctga caaccgctaa ggctatcagc
     1501 aaaaagggat agccttagga gttgatcctg gatgacagga tagtgagcca gtatctactg
     1561 ggtgtgtcca aggcgaatgt cctcttagag tgatcgtgcg cgcatgttac gccagcaaaa
     1621 ttttgccgtc aacacgcaac agaacaccac agaacaatag ttgaacttcg cgctttttgg
     1681 tactcgtcaa ggtcgctaac aggataaaaa aatcccacat ccaggaccta taatggcgta
     1741 tcggctactc attggtaaag gaatgatcac cttgaatctc aaacggattt ttctcaccct
     1801 caccttactt cccttgtttg ctgttgccgc tgatgattgc gcactctcag atccgacgct
     1861 gaccgtacag gcgtataccg ttaatcctca aacagagcgg gtgaaaatgt actggcaaaa
     1921 agccaatggc gaagcgtggg gaacgttaca tgctctgctg gcggatatta atagtcaggg
     1981 tcaggtgcag atggcgatga acggcgacat ctatgatgaa agctatgcgc cgctcggttt
     2041 gtacatcgaa aacggtcagc ggaaggtgta atgccagtca gttaag
//
LOCUS       DAJVQH010000253         2080 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJVQH010000253 DAJVQH010000000
VERSION     DAJVQH010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.257"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            116..1153
                     /gene="curA"
                     /locus_tag="OQM87_004187"
     CDS             116..1153
                     /gene="curA"
                     /locus_tag="OQM87_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCS1510070.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWGLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYLADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1351..2016
                     /gene="mcbR"
                     /locus_tag="OQM87_004188"
     CDS             1351..2016
                     /gene="mcbR"
                     /locus_tag="OQM87_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCS1510071.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          571 a          441 c          559 g          509 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gggctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga caggaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtgatgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatta
      721 cctcgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggtcc
      901 ggatcgttta cccctgttga tggcaacggt gctgaaaaaa cgtattcgct tgcaagggtt
      961 tattatcgct caggattatg gtcaccgcat ccatgagttt cagaaggaga tggggcaatg
     1021 ggtgaaagag gataaaatcc actaccgcga agatattact gatggtttag agaatgcgcc
     1081 acagacgttt atcggcctgc tgaagggtaa aaacttcggc aaagtggtga tccgcgtcgc
     1141 gggtgatgat taaagtttca acggcggcgt aagccgccat aaaaatccag gcaaaaagct
     1201 tattttcagc tttaattaac taaccacgtc attgttctgc tgctaataac aacaaaggtg
     1261 ggtggtttat gattattcac tttaatacac taggtgaact ccttctgcca tgcaggcagg
     1321 gttggacaga aaacacgcga aggaattaaa atgcctggaa cgggaaaaat gaaacatgtc
     1381 agtttgactc tgcaggttga gaacgacctg aaacatcagc ttagtattgg cgcattaaaa
     1441 cctggcgcac gcctgattac taaaaatctg gcggagcaat taggtatgag tattacacct
     1501 gtgcgtgaag cattattacg tctggtttcg gtgaatgcgc tttctgtcgc acctgcacaa
     1561 gcatttacag taccggaagt gaggcaaagt cagttagacg aaatcaatcg gatccgctac
     1621 gaactggaat taatggcagt tgccctggct gttgaaaacc tctcctcgca agacatagca
     1681 gaacttcagg aattgctcgg gaaattacag caggcgcaag aaaagggcga catggaacaa
     1741 atcattaatg cgaacaggct atttcgctta gcgatttatc atcgctcaaa tatgcccatc
     1801 ctgtgtgaga tgattgagca actgtggatc aggatgggac ctgctttaca ttatctttat
     1861 gaagcgatta acccagcgga attacgggag cgcatagaaa actatcatct attactcgct
     1921 gcgttaaaag caaaagacaa agagggatgc aaacattgtc ttgctgaaat tatgcaacaa
     1981 aatattgcta ttttatacca gcaatacaat cgttaaatta aatgcccggc aaataccggg
     2041 catcaagtaa ttactcaaat cgccacgcaa tattcatgcc
//
LOCUS       DAJVQH010000254         2049 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJVQH010000254 DAJVQH010000000
VERSION     DAJVQH010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.258"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..168
                     /locus_tag="OQM87_004189"
     CDS             <1..168
                     /locus_tag="OQM87_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCS1510072.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGIL"
     gene            153..791
                     /locus_tag="OQM87_004190"
     CDS             153..791
                     /locus_tag="OQM87_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCS1510073.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1019..1420
                     /locus_tag="OQM87_004191"
     CDS             1019..1420
                     /locus_tag="OQM87_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510074.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEISLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSSGGGR"
     gene            1431..1905
                     /locus_tag="OQM87_004192"
                     /pseudo
     CDS             1431..1905
                     /locus_tag="OQM87_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          620 a          365 c          480 g          584 t
ORIGIN      
        1 tccgctattg ccagtacacc tcacccgtcc tggcgacgta tctgtaagac actcataaag
       61 aatgattttt ggtgccggac tctctccttc agtccgaaca aaccccggca ctatgaacgt
      121 tacctgcagc gtatgaagga aaggaggaag gaatggggga ttctgtgaca ccggagatac
      181 ttggcaacat gattcgtcag tatttcagtc aggtacgaac tgaaaaagag agcatacagg
      241 ctctgaatca tctgcgcaga gtgcttcatg aggtcagtcc ttttgcacag gagcctgtgg
      301 actgtgtgtt gtgggtgaaa gcggatgagg tggtggccaa cgattacaac cctaatgtca
      361 tggcactagg tgagaaaaaa ctactgaaac agtctctgga gaaagatggt ttcacacaac
      421 cggtggtggt gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt
      481 tactggggcg ggaagcagat acaggaaaac gcctgagggg ctggttgcct gtagcctgta
      541 ttaatccgga gagaaaggga caggccgcac gtattgccgc gaccattcga cataaccggg
      601 ccagggggaa acaccagatc actttaatgt ctgatattgt cagggatctt tcacgtctgg
      661 gctggacgaa tgaacgtatt ggtacagagc tgggaatgga ccaggatgaa gtgctgaggc
      721 tgaaacagat tagcgggctg acagagttgt ttcaggaaga ggatttcagc tcggcttgga
      781 cggttcgttg atgttgttaa atatgggcgg tatttctgaa agttgatact ttgggtagaa
      841 aaaatgatac cgaaaactat ggaggatgtg cttgtcaggt gactggacac cataggtctt
      901 aggctttgat aacgggataa aatgagcggc ttttctcata ccatcaggtg tgatgatggc
      961 gcagatttac aaaatctgat tgattctaaa gatgtataag taaaaatcaa gctttgatat
     1021 gaaaaaacag atatttatta ataataaacc tcctgtagtc ccttactcag ggacgcacgc
     1081 gaaaatattt aaatacattg aaatctctct gccgtttttc tactttatct atacatcagg
     1141 cgaaccattc cacatatctg tccaaaatac tgttatttat gtatcaaaat acaatggtat
     1201 atttattaat aaattagttc ctttttctct tttatttgac agggatatat ctgttcttca
     1261 aagaagagat atttgtgttg tgcgatttac gtcagaggaa ataagtgaac ataacgtttt
     1321 atttgatcat gacattgaac gattaaaaaa aatttccaaa gctcagctta tatctcccga
     1381 ttatgttttg attgattttt ctagtggggg ggggagatga accctatgca atgtcctggt
     1441 taatatctca gtgtgctcat cagtgcactg ataataaaaa gacggagaca gatgccattt
     1501 acgataaagt tcggagttct tatttgcttt catgtattct gaaaaaaaac aaaaatgttg
     1561 ggcttattct tcatgcacca tcatttgttt ctgtgtctga aaaaaatcgc cagaattgtg
     1621 atggcaaatt actcccgtaa ctggagtaat tccgagttgg ccagtgcggt tttaatgagt
     1681 gaatcttctt taaaaagaag aatgtacaaa gaggtcggtt ctataagtac atttgtacat
     1741 aaaataaagc ttacagaagc tatcaggaaa ttacgaagga caaatactcc catttctgtc
     1801 atatcatctg agttaggtta cagttcacca tcgtattttt ctaaagtttt ctttaaatat
     1861 ttaaagacat accctcaaaa cataaggaag aaaaatggtc ggtaatgtca ccaattagga
     1921 ggacttgcag agccgctttt ttacgatact gagatcaaac agacataatc tatgcggaga
     1981 tttacagcag ggcatggttc acaaatctga cagcgatgaa ttatcggcac tgcgggcaga
     2041 aaatgctcg
//
LOCUS       DAJVQH010000255         2034 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJVQH010000255 DAJVQH010000000
VERSION     DAJVQH010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.259"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(212..376)
                     /locus_tag="OQM87_004193"
     CDS             complement(212..376)
                     /locus_tag="OQM87_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510075.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1278..1562
                     /locus_tag="OQM87_004194"
     CDS             1278..1562
                     /locus_tag="OQM87_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1510076.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1550..>2034
                     /locus_tag="OQM87_004195"
     CDS             1550..>2034
                     /locus_tag="OQM87_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1510077.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          523 a          426 c          583 g          502 t
ORIGIN      
        1 atggacatta tttttgtgga accggaggta acagaccaga cggtttaaat gagccggtta
       61 ctttaaacgg gtgatggaca gcacctcaaa agttcagatg gattatattt gtctgcaata
      121 ccccgggtat tttcgatttg cgaaatggat ggaacgactt gctcagggga tagctgatgg
      181 agttactgaa gtaccgaagg accactaaaa atcacaggcc gggaatttcg gcgtttccgg
      241 cgtaggtgtg ccacgaatgc atccagtggc gtgatgttgg atgcaatgct gtatgagaga
      301 tgaggggcgg cgtccagacg cagccttcaa accgccttga gctgctgccg ttaagagcgg
      361 tggtggtgag cgtcaaggtt aaaaggcgca gaacatgtgt caggtaggtg ttgcggagat
      421 gaacgaaagt gaactgtcga tgaagtgtcg aaaggaacct ctaggcgatg tcgaaaatgt
      481 gctatctacg cagcgcatga cgagtccagc agatatctgg tactggctgg gcggcatccg
      541 gcatataggc agcatgaacg caacacaggc tctcgtacgg aacgtgggaa cctgtcgtct
      601 cgatgctaag ggagcggcgc ggagcagagg cactgcaagc gcctgagtac cgatgcgaga
      661 cacaggggca gatggcttcg tagtagtgag gaagtgcctg taatgggcat ggagcgaagg
      721 gggtcagtcg ttggggctgg tttatcggtc aaccggatag ccgggaggag cccatgagtc
      781 agccaaagcc gttcgaggtc tctaagtatg cggtttggaa agcataccag cgggtcaaag
      841 ctaatcgagg tgcggcaggt gtcgatgggc agtcagtcga ggcgtttgag gttaccaatc
      901 gaagctatac ccggtaatgg cgcaggttga cctgcacttg gccaagtgga ttgcccgcaa
      961 acacaagcga gcgcgaggta gtctggtgcg tgcattcgag tggttactgc gaattcgtca
     1021 cgactgcccg acgctgtttg cacactggtg cttagcttac gatacttgaa tggttcaacg
     1081 acaagagccg tatgacgcga gagtgtcacg tacggttccg tgggagcgta ggggggcggt
     1141 gcccctgcgc cacccgatat cccctttgag tgcggttacg tccacagagg taaatgccgc
     1201 tgacgcggca tctgtaatct gttactttgt gtatatcatt tgctatatat tgtcaatatg
     1261 ctattgttgt aaagcaaatg atatacggag gctttatgaa atcaggtgtt cagctcaatc
     1321 tcagagcaag agaatcacag cggatactca ttgatgctgc tgcagaaatt cttcataagt
     1381 cgcgtacaga tttcattctg gaaatggcct gtaaagctgc tgaagatgtt attctcgacc
     1441 ggcgggtgtt taattttaat gacaggcaat atgaagagtt cattgaaatg cttgatgctc
     1501 cggttgcaga tgatcctgcc atcgaaaaat tgctggcaag gaaacctcaa tgggatgtgt
     1561 aactgcacca gaacctttat cctcatttca tcaggtcgct gaatttgtca gcggtgaagc
     1621 tgtacttgat gactggttaa agcagaaggg gctaaaaaat caggcaatcg gagccacccg
     1681 gacttttgtt gtatgcagaa aaggtacgca acaaatagtc ggtttttact cactggccac
     1741 tggtagtatc aatcatacag aagctacagg taaccttcgg cgcaatatgc cagaccccat
     1801 tcctgtaatt atacttgcac gcctggctgt agatgtgtca tttcgaggaa aagggcttgg
     1861 cgcggattta cttcatgatg cagttcgtcg ttgttacagg gttgccgaaa atatcggtgt
     1921 gcgtgcaatt atggttcatg cgcttacgga aaacgcaaaa cagttttata ttcatcatgg
     1981 ttttaagcca tcaaaaactc aggtgcaaac attgtttctt aaactccctc aata
//
LOCUS       DAJVQH010000256         2025 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJVQH010000256 DAJVQH010000000
VERSION     DAJVQH010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.261"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..364)
                     /locus_tag="OQM87_004196"
     CDS             complement(<1..364)
                     /locus_tag="OQM87_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1510078.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(431..1645)
                     /locus_tag="OQM87_004197"
     CDS             complement(431..1645)
                     /locus_tag="OQM87_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCS1510079.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          628 a          492 c          413 g          492 t
ORIGIN      
        1 ccagaccgat acgcattaaa ataaagtggc taaaagaatt aaccattcct gttaatgcct
       61 gcattaatga ccagaatatt aagcctgcgc ctaaaactat tcgtgggcca aagcggtcta
      121 gcaaaatacc ggaaggtagt tgtgaaaaac cataagacag agaaaatgca gaaagcaaaa
      181 caccaaactc tgtcgccgat aatcctaact cgccacgaat cgcttcgcct gccacgctca
      241 atgacgaacg gtcgagaaaa ttaacgatcc ccgccataaa taataaaacc agggtcactg
      301 tctgaatacg acgaatccgt tgcggtttat tacccgcaac actggttgac acatcaacgt
      361 ccatttttag ctccttgagc ggaattaatc gatttgatga ctattcagaa aataattaag
      421 cgaaccgtta ttaccagttc aacaatgtgc catcttccag tcgtgcgacg gggagatact
      481 ccgggtcata cggatatttc gccgccagtt tctcatcgaa agaaataccc agccccggtt
      541 catcgcctgg gtgcatataa ccttcctcga agcgccagct gtgcgggaaa acttcaagca
      601 tttgttcgga atagcccatg tactcctgaa cgccaaaatt tggtacccat aggtcaaaat
      661 gcagcgccgc cgcatgacaa acaggcgata aatctgaagg gccatgcgaa ccggtacgta
      721 cctgataaag cgatgcaaaa tcggcaatcc gccgcatccc ggtaatgccg cctgcatggg
      781 tgatcgtcgt gcgaatatag ccaatcagct gctcttcgat taactgcttg cagtcccaga
      841 tactgttaaa cacttcaccg accgcaatcg gcgtcacggt atgctggcga atcagacgaa
      901 aacattcctg gttctcggca ggcgtcggat cttccatcca gaacaggcgg taatcctcaa
      961 tgctcttccc aaagcgcgcc gcttcgatgg gggtcaggcg atggtgcatg tcatgcaaca
     1021 gatgttcgtt aaaaccgaac ttattacgca ccgcttcgaa taacttcggc gtaaaatcga
     1081 gatacttttc tgaagaccac aactgctctt ctggccacag gcctttggtt gccggttcat
     1141 acgccagtcc tttgcccttc gccaggccat aagtggtttg catacccggt acaccgcact
     1201 gcacacgaat cgccttaaag cccatctgct ggtgctttgc atagtcttca aggacttcat
     1261 caatcgtacg cccggtggtg tggcaataaa ccatcacgcc ttcacgagaa gcaccaccaa
     1321 gcagttgata caatggcata tttgccgctt tagctttgat atcccacaac gccatatcaa
     1381 tggcagaaat ggctgacatc gtgacggggc cacgtcgcca gtaagcacct ttataaaaga
     1441 attgccagat atcttcgata cggctggcat cgcggccaat tagttgtgga caaagatgat
     1501 ctttcagata agaagcgacc gacaattcgc gaccatttaa cgtggcatct cctaatccac
     1561 aaagtccgct ttcagtggtt attttaagcg taacgaaatt acgccctggg caagtaacaa
     1621 aaacatctgc agcaataatt ttcattattg caatctcctg tgttgtaaat agacgtttca
     1681 aacctaattt tacgcctact accatacaag agagaaaaag cgattattca accgtgatca
     1741 cattaaaaac aaaaatttcc accgaggaga aaaaataccc tcaaaaacca cctgaataat
     1801 taaccattat tgttaaaaca acacgttaaa taatcacata acaaactcca ttaatcattg
     1861 aaaatgagca cagaaataga aattaattat attcgattaa ttaaaatgtg tattgcttga
     1921 tgtatttaaa aatattattt tcatggtgag agtggaggta aagaagacac aataataaaa
     1981 aggaatgtga caaataatgg ttctggttgg gcgttaaatt taagg
//
LOCUS       DAJVQH010000257         1998 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJVQH010000257 DAJVQH010000000
VERSION     DAJVQH010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.262"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..483)
                     /locus_tag="OQM87_004198"
     CDS             complement(<1..483)
                     /locus_tag="OQM87_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1510080.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     Y"
     gene            831..1703
                     /gene="rluF"
                     /locus_tag="OQM87_004199"
     CDS             831..1703
                     /gene="rluF"
                     /locus_tag="OQM87_004199"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCS1510081.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(1836..>1998)
                     /gene="yjbD"
                     /locus_tag="OQM87_004200"
     CDS             complement(1836..>1998)
                     /gene="yjbD"
                     /locus_tag="OQM87_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCS1510082.1"
                     /translation="ETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWS
                     ANTSTRGRR"
BASE COUNT          485 a          501 c          505 g          507 t
ORIGIN      
        1 gtaggttaat gtgtttacat gatagcggct ttcgagttta ccggacggtc cgccaataat
       61 tggtacgcag attaactgcc gcgattgccc cgcctgcggc aagttttcaa ccagtgcgct
      121 caccgcgcga ccccaggaaa aaccgacaat atcgccaggc tccagcaagc gatccagcag
      181 ttgcgcgccg tgtaacccca tcatcgccag ttgagtctct tcatcctcat catttcccga
      241 caccaccaca acgtctttca ggccaaactt ttgcttcagt tgctgctcca gccagagatt
      301 ttcgttgtag tcatagttga tggcgatggt gaccattccc tgatcgcggc ctcgtttaag
      361 caagcggctg atggtggtgc ggtaaatacc cagttcgcgc gcgatttgcg cctgcgtcat
      421 atcctgttcg taatagagtt gggcaatctt cacaatcaaa cggatatcgt cactgttttc
      481 cattgccgcc tcctggttct gcacatttgt gcaaatacgc tttctcaccg cttgatctga
      541 tataacccca tcatgagggc ctttcaaagc gatttttcac tctttgcatg gggagaaata
      601 gcgctttctc ttgcacattt gtttctctgc gtagtagtca tcagcacagg tgtgcagcga
      661 ttcgtaatat gttgattatc cttattttgc caggcaaatt tgcatcgtac ttccggagac
      721 acttttgtga taaaaggcgt ggcagatcac atttaatttc gacgttgccg cataaccctg
      781 tttttccccg ctaagaggta cggtatattc cgcgttttac aggagaattt atgctgcccg
      841 actcatcagt ccgtttaaat aaatacatca gcgaaagcgg aatttgctca cgccgcgaag
      901 cggatcgcta tatcgagcaa ggcaatgtgt tccttaatgg caagcgagcc accattggcg
      961 atcaggtgaa acccggcgac attgtgaaag taaacggtca gttgattgag cctcgggaag
     1021 ccgaagattt ggtacttatc gccctgaaca agcccgtcgg tattgtcagc accaccgaag
     1081 atggcgagcg cgataacatt gtcgatttcg ttaaccacag caaacgcgtg ttcccgattg
     1141 gccgcctgga taaagactcc caggggctga ttttcctcac caatcacggc gatctggtga
     1201 ataagatcct ccgtgcgggt aacgatcatg agaaagagta tctggtgacg gtcgataaac
     1261 cgattaccga tgaatttatt cgtggtatgg gggcgggggt gccgatcctc gggacggtga
     1321 cgaaaaagtg caaagtcaaa aaagaagcgc cgtttgtctt ccgcattacc ctagtgcagg
     1381 ggctgaaccg tcagatccgg cgcatgtgcg agcatttcgg ctatgaagtg aaaaagctgg
     1441 aacgtacgcg cattatgaac gtcagcttaa gcggcattcc gctgggggag tggcgcgatt
     1501 taaccgacga tgagttaatc gacctcttta agctcatcga aaattcctct tccgaggcaa
     1561 aacctaaagc gaaggccaaa ccgaaaacag cgggcatcaa acgtccagtc gttaagatgg
     1621 aaaaaacggc ggaaaaaggc ggtcgcccgg cgtccaacgg taagcgtttt acctcgccgg
     1681 ggcgtaaaaa gaagggacgc tgattaacgc ctcagactga tgaaaacgtc caaaaagagc
     1741 tcggacagtc ccctcgcccc ttcggggaga gggttagggt gaggggaaga ttgtgtccac
     1801 acagacgagt gaaaatctca ggcgttgatt gttcattagc gcctgccgcg cgtcgacgta
     1861 tttgctgacc agctaaatga cgcttcagga tccggtttat aagcctgctt tttcttcaat
     1921 tggcgggctt ttttcgcttc agcctcacgc tcgaccatta atttatcgat gtactctttc
     1981 ttaatgctgt tggtttcg
//
LOCUS       DAJVQH010000258         1930 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJVQH010000258 DAJVQH010000000
VERSION     DAJVQH010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.263"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            70..630
                     /locus_tag="OQM87_004201"
                     /pseudo
     CDS             70..630
                     /locus_tag="OQM87_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            745..1086
                     /locus_tag="OQM87_004202"
                     /pseudo
     CDS             745..1086
                     /locus_tag="OQM87_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1069..1906
                     /locus_tag="OQM87_004203"
                     /pseudo
     CDS             1069..1906
                     /locus_tag="OQM87_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          480 a          467 c          585 g          398 t
ORIGIN      
        1 taaaatatta tcagatgact gcttagaaaa agaacaaatg gtggtgtccg ctattgccag
       61 tacacctcag ttctgggcct tgcccgtagc agttattttt atcaccgggc tgcactgaaa
      121 gccggtgata aatacgccac gatacgtaag atgctgacag atatcacgga gttccagctc
      181 cctgcaggta aagtctggct gtcaccagtg gtggactgct tcgatggaaa agttgtgagc
      241 tggtctctca gtacacgccc tgatgctgaa ctggtcaaca ctatgctgga tagcgctgtc
      301 gaaacgttaa atgctggcga acgaccggtg atacacagtg acagaggtgg gcattatcgc
      361 tggccaggct ggctggaaag agtgaatgca gcaggtctta ttcgctcaat gtcccgtaaa
      421 ggatgttcac ctgataatgc tgcatgcgaa ggctttttcg gcagactgaa aacggaaatg
      481 tattatgggc gtaaatggtc gggcatcacg ccagaaaagt tcatgcagca agtggatgct
      541 tacatcagat ggtataacga gcggcgtata aaattatcgc tgggtgccgt cagccccaaa
      601 atgtaccgcc aacaatgcgg gctggaatga taaagcagtc caggaaatcg tccgcatccc
      661 cccttcacca tttggattgt ccgctccacc caacatgttg tttccttaag gttcctacac
      721 cacaaaggaa actctcatgc tgcgcagtgg atggctgacg ttgccgacag gcgggaactt
      781 cgccagttca ggcagacacc ggaacagcgc ttcacgcagg aacaggagca tctgcagccg
      841 ctgctgggta cggacttcga tatccgtcat gtctcctggg atggctatat cgaggttggc
      901 gggaaccgtt acagtgtgcc ggaaagcctg tgtggtcagc tggtatcaat acggatctcg
      961 ctggatgatg aactgcggat ctacagtaat gagcaacagg tagcatcgca ccgtctctgt
     1021 tcggctgcat acggctggca gaccgtgccg gagcatcacg ctccactctg gcagcaggct
     1081 tcggagcgca tggccgaacg gctgggggag atacagaaac gggtaatcac tgtctgtgac
     1141 cgggaagcgg atatctggca ttacctgtat tataaagtca gccacggaca gcgtggtgcg
     1201 tgctgcacag aatcgccggc tggaagaggc acccggcaag ctcttcgaac tgccggaagt
     1261 cctggcaacc gcaggaagcc acacgctgaa tgtgatgcaa aaaggcgggc gggcagcccg
     1321 tcaggcccgg atgttcatca gttacagcga agtcagcata aaaaatcccg acaacagcgg
     1381 ccaggcgctc ccgctcacgt atgtctgttg ccgggagcag gcagaggacg gtgcctgctg
     1441 gcatctgctg acgtcaggaa aagcggcgag tgccgccgat gcacgacgta ttgtcagcca
     1501 ttacgagcga cgctggctga cagaggaata ccacaaggcg tggaaaagtg gtggtacatg
     1561 gaatcgctgc gaatgcagac ccgggataac cttgagcgca tggtggttat ccaggcgttt
     1621 atagcggtac gggtgctggg cctgcggcag ggaggtgtca gtgaagaaac acaaaatgac
     1681 agctgtgaga aaatactgac gccgacagag tggaaactac tgtgggttaa gctggaagga
     1741 aagccgcttc cggtgcaggc accgaccctg aaatgggcct ggggcgatgg catgacagca
     1801 aacgcacagg tcgtcccggt tggggcgtca tgtgggatgg ctggtcagac ttcaggatat
     1861 ggttgagggt tacctggtga tgaagtctct tgatcaggag ctctgatcaa gagacagcag
     1921 cttagggggg
//
LOCUS       DAJVQH010000259         1881 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJVQH010000259 DAJVQH010000000
VERSION     DAJVQH010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.264"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..1481
                     /locus_tag="OQM87_004204"
     CDS             <1..1481
                     /locus_tag="OQM87_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1510083.1"
                     /translation="FAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIVEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            1489..>1881
                     /locus_tag="OQM87_004205"
     CDS             1489..>1881
                     /locus_tag="OQM87_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510084.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTG"
BASE COUNT          521 a          368 c          459 g          533 t
ORIGIN      
        1 cgtttgcgtt cattattatc gtgttgctgg tggtatattt catgaccact cgtgtgttcc
       61 gtcggcaatt ttctgaaatg accgagctgg ttaatacgct ggcgtttttg cccgactcaa
      121 cggatcagat cgaggctctg aaaattcgcg aaggcgatgc gaaagagatt atcagcatca
      181 aaaattcgat cgtggaaatg aaagatgccg aaattgaacg gtcaaataaa ttgctctcac
      241 tgatctctta cgatcaggaa agcggtttta ttaaaaatat ggcgattatt gagtccaaca
      301 ataatcagta tctggctgtg gggatcatca aactgtgtgg tctggaagcc gtggaagcgg
      361 tgtttggtgt tgatgaacgc aataaaatcg tcaggaaatt gtgtcagcga attgccgaga
      421 aatatgcgca atgctgcgat atcgtgacat tcaatgccga tctctattta cttctgtgtc
      481 gggaaaatgt acagacattt acccgtaaaa tagcgatggt aaacgatttt gacagcagct
      541 ttggctaccg caatctgcgc atccataagt ctgccatttg tgaacctttg cagggggaaa
      601 acgcctggag ttacgcagaa aaactgaaac tggcgatttc cagtatccgt gaccatatgt
      661 tctccgagtt tattttctgt gatgacgcga aactcaacga aatagaagag aatatctgga
      721 ttgcgcgtaa tattcgccat gcaatggaaa ttggcgaact attcctcgtc tatcaaccga
      781 tcgttgatat taacacccgc gccattctga gcgcggaggc gttgtgccgt tgggtttctg
      841 cggagcgggg gatcatttca ccgctgaagt tcattaccat tgctgaagat atcgggttta
      901 tcaatgagct gggttatcag attattaaaa cggcaatggg tgaattcaga cattttagtc
      961 aacgtgcgtc gctgaaggat gatttcttac tgcatattaa tgtttcgccc tggcagttaa
     1021 acgaaccgca ctttcatgag cgttttacca ccatcatgaa agaaaatggc ctgaaggcga
     1081 acagcctctg tgttgagatc actgaaaccg tgatcgagcg aattaatgaa catttttatc
     1141 tcaatattga acaactgcgt aaacaagggg tacggatatc gattgatgac tttggcaccg
     1201 gtttgtcaaa cctgaaacgt ttttatgaaa ttaatccaga cagcataaag gtggactcgc
     1261 aatttaccgg cgatattttc ggtactgcgg gaaaaattgt gcgcattatt ttcgatctgg
     1321 cacgctataa ccggatcccg gtgattgcgg aaggcgtaga gagcgaagac gttgcgcgcg
     1381 aattaattaa attaggatgc gttcaggctc aggggtatct gtaccagaaa cccatgccat
     1441 tctccgcctg ggataaaagt ggaaaattag taaaagagta gtttacgtat gtccagaatc
     1501 aataagttcg tacttacagt cagtctgctg atttttatca tgatttcagc agttgcctgc
     1561 gggatctaca ctcaaatggt aaaggaacgg gtgtatagcc tgaaacagtc cgttattgat
     1621 actgcttttg cggtggcaaa tattgctgaa tatcggcgta gcgtggcaat tgatcttatc
     1681 aacacgctaa atcccacgga ggaacagctg ttggttggtt tgcgcacagc ttacgccgac
     1741 tcggtttccc cctcttattt gtacgatgtc ggtccttatc tgatttccag tgacgaatgt
     1801 attcaggtaa aggagttcga gaaaaattat tgtgcagata ttatgcaggt tgtgaagtat
     1861 cgacatgtca aaaatacagg g
//
LOCUS       DAJVQH010000260         1804 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJVQH010000260 DAJVQH010000000
VERSION     DAJVQH010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.265"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..386
                     /locus_tag="OQM87_004206"
     CDS             <1..386
                     /locus_tag="OQM87_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1510085.1"
                     /translation="VDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYGEQV
                     SKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITYDRY
                     AKEVEVETLKEKLAESLSVKKIDGK"
     gene            418..1026
                     /locus_tag="OQM87_004207"
     CDS             418..1026
                     /locus_tag="OQM87_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510086.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKSVLAVNSEQFRGLTVERLAKYIEMA
                     KHIDHEISDNGYRPSEGYLPAAERRSLMLAFGVSGVSMWPSA"
BASE COUNT          509 a          382 c          444 g          469 t
ORIGIN      
        1 tggtagatgg tgcttatggt tttgtattgg ctgactttat gtcactcgtg gaagaccgta
       61 gaaagactga gggtgataat gctatggtgt tcaatggcct caagctaatg aagaacggct
      121 acggtgagca ggttagcaag tggtttaacc gaacgttgct gcccaaagtc ctcgctgacc
      181 gtagcggctt agcttttcac tcgtttagac atacagttgc gacccagtta aaacaacatg
      241 gggtcgagtt agcgtatgct caggcgatta tgggtcatag ctcagggtct ataacttacg
      301 accgttacgc gaaagaggtc gaggtggaaa cgctgaaaga aaaattagcg gagtcattgt
      361 cagtaaaaaa aatagacgga aagtaatcgc atagggatag cggaatgtga ggacgtaatg
      421 gtcaaagaat ttttagttag caaagaaaat aagccttcat atgaatgtat gaatactgct
      481 gaaacttgtt tggggctggc tggaatggct tcggaagatt ggcaacgtga gcatcattgc
      541 cgcacaggta taatatttac tgcttttgct attgaggcaa tgttcatctt ctatagaaaa
      601 caggttgacc ccggctacga taagacgcag aaagagtgca gaaagacaat gcacaagaat
      661 acgttaaaat tgtgcggtat aaacaattat atggggacaa aaccttacca aattatcaaa
      721 gagtgtcttg aggtaagaga cgctattgct cacggcgatt cgtatacttc gagctttaat
      781 ttctcggcgg atcatttgga taaccaagac gatattgcaa aaagtgtcct ggcggtaaac
      841 tctgaacaat ttcggggatt aactgttgag cgccttgcaa aatacattga aatggcaaag
      901 catattgacc atgagatttc tgataatggt tacaggcctt ctgaaggtta ccttccagca
      961 gccgagcgca gaagtctgat gttagctttt ggcgtcagtg gtgtgtcaat gtggcccagt
     1021 gcttgagcga taatacacct cactatagag gaatggaccc ttaactatca ctatagggga
     1081 gagaccctaa gtccccacct taatagcagt cacgacccat acacacattc attaagtctc
     1141 aggcttccgg tagcggaccg attggaaacc atccgcagac gcctagtgtt aaatcacctt
     1201 ggcctgagac cattcctttt aacgtaagct atcttccctt ttgacaggcg gggagcagta
     1261 gaccacatcc gactttaagt tggctctgtg ataatcgctg cgcggataac tgaagaacca
     1321 agacaccatt actgaggtct gccatccatt tgtggacagg cggaaactca aagaggttag
     1381 atcagtggtg tctgaggttg gagctttgag tcttgaccag caggctggag gagaccatga
     1441 gtttatcttg ttatggattg tgttatcaca attccaataa gaagaacttt aagtccagcc
     1501 tccactcccg ccaacccgaa gtaaaattat ggtcgctatg atgtggtctc catgcttcgg
     1561 gtcttactgg aagtactgaa cactttcgcg tcaagttctc cgaagttcat tcctgtttga
     1621 ctaaactatc actataggga ccatatctcg cctctgacct cctgagagac ggcaagcgag
     1681 aaaccgtggt ccacgctagc aagctagcca ccagcctaag gcaagacagc ctctggtcag
     1741 cctgtgtgtt ctttggtctc gtgacttaag tctatcacca cacacgctcg gacatgctgt
     1801 cgaa
//
LOCUS       DAJVQH010000261         1769 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJVQH010000261 DAJVQH010000000
VERSION     DAJVQH010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.266"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(185..382)
                     /locus_tag="OQM87_004208"
                     /pseudo
     CDS             complement(185..382)
                     /locus_tag="OQM87_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125062.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(514..>1769)
                     /locus_tag="OQM87_004209"
     CDS             complement(514..>1769)
                     /locus_tag="OQM87_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1510087.1"
                     /translation="LKQGIVWCTSLPGNRVLLSRIPVVPDSNLLLAPAIDTVNRLPIL
                     LYQSQFADTRILVTISDQHIRGALNVPLKGVRYVLRVADDIIGPTGDVMTLNGHYPYT
                     EKVHSTKYHFTIIFNPPPLFSFYRLIDKGFGILIFILLIACAAAFLLDRYFNKSATPE
                     EILRRAINNGEIVPFYQPVVNGREGTLRGVEVLARWKQPHGGYISPAAFIPLAEKSGL
                     IVPLTQSLMNQVARQMNAIASKLPEGFHIGINFSASHIISPTFVDECLNFRDSFTRRD
                     LNLVLEVTEREPLNVDESLVQRLNILHENGFVIALDDFGTGYSGLSYLHDLHIDYIKI
                     DHSFVGRVNADPESTRILDCVLDLARKLSISIVAEGVETKEQLGYLNQNNITFQQGYY
                     FYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          549 a          391 c          364 g          465 t
ORIGIN      
        1 tattaatagt tgccatggta attatttcgg tgatatataa aaacaactgt gataacacca
       61 aggttgacaa ctatttgcgc aataattcct ttccttcaat gatccaggcg ttcactgcca
      121 gcaacccatt cagccgcata ctgacagtga gaaactgaaa gattattgct gatccctgtg
      181 gctgacgagt ctttctctta aatcgattaa ccagattgat cgccagccaa acaagggcat
      241 taagcaaatt tatcgtcaca agcacttctc ccagcccggt actgggtttt gcaataagga
      301 tgaccaggag ggtgatcagc ataaatgcaa tggaaaagat gatatagaga gtgaaataat
      361 ccagattatt actattgttc atgctactaa tggcaatcct gtcaaaactt tcaaacaatt
      421 atagcaaaca aaataatgaa caatagtaac aagaggcaac gagctaatta gctgtctata
      481 attacaccgc actatggcct tactgatcaa ttttcactca accacaacct tcaccttcgg
      541 tttagaaagg ataattttga ccaggtcgat gtatgtaaca ggtttataga aataataacc
      601 ctgctgaaat gtgatattat tttggttcag atagccaagt tgttctttcg tttcgacacc
      661 ttcagcgacg atactgatcg aaagtttacg cgccagatcc aatacacaat ccagaattcg
      721 ggttgattct gggtctgcgt ttacgcggcc aacgaaactg tgatcaattt tgatataatc
      781 aatatgcagg tcatgaagat aagaaagccc tgagtagcca gtaccgaaat catccagcgc
      841 gatgacaaaa ccattttcat gcagaatgtt caaccgctga accagacttt catcaacatt
      901 caatggctca cgctcggtga cttccagaac aaggtttaaa tcgcggcggg taaaactgtc
      961 acggaaattt aaacactcgt cgacaaacgt cggcgaaata atatgcgaag cgctaaaatt
     1021 aattccaata tgaaaacctt ccggcagttt actcgcgata gcgttcatct gtctggcaac
     1081 ctgattcatc aggctttgtg taagcggaac gattaatccg gatttttcag caagtggaat
     1141 aaatgccgcg ggtgaaatat atccaccgtg aggttgtttc cagcgggcta acacctcaac
     1201 tccccgcaat gtcccttccc gaccatttac cacaggttgg taaaaaggga cgatctcccc
     1261 attatttatt gcccgtcgca ggatctcttc aggcgttgcg cttttattga aatatctatc
     1321 aagcaggaag gcagcggcgc aggcgattaa cagaataaat attaaaatcc caaagccttt
     1381 atcgataagt ctgtagaagc taaagagtgg cggcgggtta aagataatag tgaaatgata
     1441 ttttgtggag tgaaccttct cggtataggg ataatgtcca ttaagcgtca tcacatcacc
     1501 cgttggtcca ataatgtcat ccgccacgcg taatacatac cttacccctt tcaagggtac
     1561 attaagcgcc ccgcgaatat gttgatcact tatcgtaacc aaaatgcgcg tatctgcaaa
     1621 ttggctctga tagagcagga taggtaatct attaacggtg tcgattgccg gagccaacag
     1681 taaattactg tccgggacaa caggaatacg agacagcagg acccgattcc caggcaggga
     1741 tgtacaccag actattccct gtttgagaa
//
LOCUS       DAJVQH010000262         1752 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJVQH010000262 DAJVQH010000000
VERSION     DAJVQH010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.267"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            62..1371
                     /gene="ltrA"
                     /locus_tag="OQM87_004210"
                     /pseudo
     CDS             62..1371
                     /gene="ltrA"
                     /locus_tag="OQM87_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          444 a          404 c          526 g          378 t
ORIGIN      
        1 tacagggacg gagtaaaccg tagtagcggt gaaggaccgt aatgggtccg gagcaaaggg
       61 cttgccccat tgtgctgatg gtgcggcggc aaccacgggc gacaatgccg atgggaggac
      121 tgctgtaaaa tcggcaaagc catttcctgt aagtaaacga caggtatggg aagcttataa
      181 acgagtaaaa gccaaccgtg gggcggcagg cattgacggg cagacactgg ccgggtttga
      241 cgagaatgtt acagacaatc tctacaaact gtggaacaga atggcttccg gcagttatat
      301 gccgcaggcg gtaaggcgtg tggacattcc caaagctgac ggtggtgtac gtccgctcgg
      361 tattccggcc gtgtcggacc ggatcgccca gatggtcgtt aagcagatac tcgagccggt
      421 actggaaccc ctgttccatg cagactcgta tggttatcga ccgggtaaat ccgcacacca
      481 ggcgattgcg caggcgagga aacgctgctg gaagtttgac tgggtggtgg aagtcgatat
      541 taaaggcttc tttgatgata tcgatcatga cctgctactg aagacagtgc agcatcatac
      601 tcaggcacgc tgggtggtga tgtacattga gcgcaggctg aaggcaccgg tgcaaatgcc
      661 ggatggagca atgctggcac ggggtcgagg aacgccgcag ggcggcgtaa tctcccccct
      721 gctctcaaat ctgttcctgc actatgcctt tgatatgtgg atgcaacgtc agtttcccgg
      781 cgttcccttc gaaaggtacg ccgatgatgt tgtatgccac tgtcacagcc agtggcaggc
      841 tgacgctctg ataagtggac tgaggcagcg gcttgcgcaa tgcgggctgc agctgcatcc
      901 gcaaaaaaca cgaatagttt actgtaagga tgctgaccga cggggggact acccggaaac
      961 gagcttcaac tttctgggct atacattccg gccgcgtctg tcgatgaatc gctggggtaa
     1021 gaccttcgtt aacttcagtc cggcgatgag tgccagagca ggaaaagcaa tccgtcagga
     1081 ggtgaggaga atcgcagtga caagtccttg tacgagctgg cgcatatgtt caatgcgaaa
     1141 atcaaaggct ggataaagta ctacggtgct ttttataaat cagcactgta tctgacgctg
     1201 aggcaaatcg atcgcaaact cgtgttatgg ctgccccgta aacacaagcg acttaggggc
     1261 caccgacgac gggccagcca ctggctggcc cgtgttgcgc ggagtgaaac gaggttattt
     1321 gcacactggc cgttactatg gggacaggcc tcaatgagaa gagccggatg agctgagagg
     1381 ttcacgtccg gttccgtgag caccagaggg ggaggttccc tcgggtgact cgacttgtag
     1441 tacattgttc acgaatgagt gatgcaacaa ggctgaagaa caggttatcg gtgcgcttct
     1501 cggaagtcgg gttggtcctg aacgcaggga agacgaatat cgcctacatt gacacgttta
     1561 aaaggagaaa cgtcgctacg agtttctcct ttctcggata tgacttcaaa gtgcgtactc
     1621 tgaagaattt caaaggcgaa ttgtacagaa aatgtatgcc gggtgcatca aatgcagcaa
     1681 tgtgcaaaat aacagaaaca atcaagaagt ggcgtataca tcgctcaaca gctgagagtt
     1741 tgctggattt tg
//
LOCUS       DAJVQH010000263         1718 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJVQH010000263 DAJVQH010000000
VERSION     DAJVQH010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.268"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..387
                     /locus_tag="OQM87_004211"
     CDS             <1..387
                     /locus_tag="OQM87_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1510088.1"
                     /translation="LHFNKNTGSCPAVQFRVNYKNGGIFYRSARDGYGFEAGWSEFYT
                     TTRKPSARDVGAYTQAECNSRFITGIRLGGLSSVRTWNGPGWSDRSGYVVTGSVNSNR
                     DELIDTTQARPIQYCINGTWYNAGSI"
     gene            390..923
                     /locus_tag="OQM87_004212"
     CDS             390..923
                     /locus_tag="OQM87_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1510089.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQVENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(952..1479)
                     /locus_tag="OQM87_004213"
     CDS             complement(952..1479)
                     /locus_tag="OQM87_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1510090.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEECARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1481..1718)
                     /locus_tag="OQM87_004214"
                     /pseudo
     CDS             complement(1481..1718)
                     /locus_tag="OQM87_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171265.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          480 a          381 c          383 g          474 t
ORIGIN      
        1 ctccacttca ataagaatac ggggagctgc cctgctgtac agttccgtgt gaattataag
       61 aacggcggta ttttttatcg ttcagcgcgt gatggttatg gatttgaggc tggctggtca
      121 gagttttaca ccacaacacg caagccatca gcgagagatg ttggcgcata tacgcaggca
      181 gaatgtaact caaggtttat tacaggtatt cgccttggtg gtctgtcatc tgttcggaca
      241 tggaatggtc ccggctggtc tgacaggtca ggttatgttg ttaccggttc cgttaactca
      301 aacagagatg aactaattga tacaactcag gcaaggccaa ttcagtattg cattaatggg
      361 acgtggtata acgcggggag tatttaacga tgatgcactt aaaaaacatt tctgctggta
      421 atcctgaaac aaaagagcaa taccagctaa cgaaacaatt taacatcaaa tggctttata
      481 cagaggatgg gaaaaactgg tatgaggaac aaaagaactt tcagcctgat acgttgaaaa
      541 tggtctatga ccacaacggc gttattattt gtattgaaaa ggatgtttca gcaattaatc
      601 cggaaggcgc aagcgtcgtt gaggttcctg atattacagc aaatcgccgg gctgatattt
      661 caggtaaatg gatgttcaaa gatggcatag tgataaagcg aacttatacc ggggaagagc
      721 agaggcaaca agtggaaaat gaaaagcaaa gcctgttgca acttgtcagg gataaaaccc
      781 agctatggga ctcacagcta cgactgggca tcatttccgc cgggaataag cagaaattaa
      841 ccgagtggat gctctacgcg cagaaggtcg aatccacaga cacctccagt ctgccagtaa
      901 cgtttcccga acaaccagaa tgagagaagg cccgctatcg ggccttaatt tttactctgg
      961 tttttgtggc cattctggct ttgccgtatc cacacggctg accagaacac tgtagcgttc
     1021 ccatgactcc agtcgtgcgc attcctcatc tgttgccata ttcagcctga cagagcgctc
     1081 cagcggctgg atgactgatt cagcttcgga aagcaatgcg gctttttgtg attcggcctg
     1141 ttgctgctgt tcgtctgccg tataaatccg cttaaccaca gctccgtcct taaacatcca
     1201 cttcccggaa tcatcggcac gacgatttgc tgttatatcg gggacctcaa cgacgctaaa
     1261 gccttcagga ttaagcgtgg aggcatcttt agtgatggcg acaataatat tattttcatc
     1321 gtaaacaatc tttattgtat ctggctgaaa gttcttcact tccccatacc agttttttcc
     1381 gtcttctgtc cacaaccaga ttacgtcaaa attctttgtt aattcgtact gttccagtgt
     1441 tttagcgtta cccgctttaa tgttctttaa gtgcatcata ttaaacgctc gctacattat
     1501 accaagtgcc atttatatac ttttgaatcg gtctgtaata aacacccgca atattatcgg
     1561 cagagttgaa cctgtatcct gaacattaat accagacaat acatgacctg aagggcactg
     1621 aaaattccat gtttgccagt tgttcactcc ataatattgc tgtgaaccaa gtcgaacatc
     1681 tttcacatat ctggaatcaa aattgccata gttgccgg
//
LOCUS       DAJVQH010000264         1702 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJVQH010000264 DAJVQH010000000
VERSION     DAJVQH010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.269"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..444
                     /locus_tag="OQM87_004215"
     CDS             <1..444
                     /locus_tag="OQM87_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1510091.1"
                     /translation="PGEQTATSILKQRTKNLAPLPHAHQQQNPPQEETVVSIAVDPES
                     PAQYLQRQKPQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTKADDLFVI
                     PLCRKCHNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            445..810
                     /locus_tag="OQM87_004216"
     CDS             445..810
                     /locus_tag="OQM87_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCS1510092.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            807..1523
                     /locus_tag="OQM87_004217"
                     /pseudo
     CDS             807..1523
                     /locus_tag="OQM87_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
BASE COUNT          485 a          353 c          464 g          400 t
ORIGIN      
        1 ccgggagagc agaccgccac cagcatactg aagcagcgca caaaaaatct tgcgccgctg
       61 cctcacgccc accagcaaca gaatccacca caggaagaga cggtggtcag cattgccgtt
      121 gatccggaat ctccggctca gtatctccag cgccagaaac cacaacggga agagatgcct
      181 gtatacacgc gctgggtaaa aacgcagaaa tgcatgacgt gtggcaatca ggcagatgat
      241 ccgcaccaca tcattggtca tggactggga gggatgggaa caaaggctga cgatttgttt
      301 gttattccgc tgtgtcgtaa atgccataac gaactacacg ccggggtaaa agattttgaa
      361 gaaaaacacg gtagtcagtt gttgttgctg attcgttttt taatgcacgc gagaaattcg
      421 ggtgttctga agtggaaagc ataaatgacc gaacgcatag aatttgtttt gccttacccg
      481 ccaacggtga acacctactg gcgacgtcgt ggcagcacat attttgtatc aaaagccggt
      541 gagcgttatc gccgtgatgt ggcgctaatt gttcgccagc agcgactgaa attaaacctg
      601 tccggaaggc tggcgataaa gattattgca gagccaccgg ataagcgccg tcgtgacctg
      661 gacaatatcc tgaaggcacc gctggatgcg ctgacgcatg ctggactact tatagacgac
      721 gagcagtttg atgaaatcaa tattgtgcgc ggtcagctcg ttcctggtgg gcggctgggg
      781 ataaaaatca cagaactggg gtgcgcatga ataaccagta tttacagttt gtgcgtgagc
      841 agctcattat cgccaccgct gatttgagtg gggcaacaaa aggtcagctt gaagcctggc
      901 aagagaatgc catgttcgat acagggcgtt acaggcgtaa aaaaatccgg taccgcgatg
      961 aagtgactgg aaaaatgata acgcgggata atccaccaat cccgggaaag caatcgctgg
     1021 cgaaggggac gtcaattcct ctggtcagtc cggttgagtt ttcgacatca tcgtggcggc
     1081 gggctgttct gtctcttgaa gaacatcata aagcctggtt gttgtggtgt tacagcggga
     1141 gtatttgttg ggaatatcag atcgcgataa cacagtgggc gtggaatgaa tttaatgctc
     1201 aatccggtac cagaaaaatt gcagggaaaa cgcaggaacg cctgaaaaaa ttaatctggc
     1261 tggcggcgca ggcagtaaaa gcagaacttt ttggtgggga aggttatgaa taccaggttg
     1321 gtggggaagg ttatgaatac caggagctgg catttactgg cgggagtgac aactaaaaac
     1381 tggtccaaaa catttactcg tcactggatt gcaatgaaac acatttttca ccgactggat
     1441 agtgaggctt tattgtttgt aatgagaaca cgttcaaaaa aaagacggca ttttcaaagc
     1501 aaagtgttgc aaaagtagat tgaaaggcat atattcatgc aaatctgata ttttgccgat
     1561 tttttacgtg atggcaaaag caaacaaaac ccgcccacaa gcgggttttt tgtgccactt
     1621 atctcggata gacatggtga atgcgctggt ggaggagata agggtgattt ttgaatgctt
     1681 gcaacattga tttcgtaacg tt
//
LOCUS       DAJVQH010000265         1680 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJVQH010000265 DAJVQH010000000
VERSION     DAJVQH010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.270"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(605..941)
                     /locus_tag="OQM87_004218"
                     /pseudo
     CDS             complement(605..941)
                     /locus_tag="OQM87_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          545 a          335 c          326 g          474 t
ORIGIN      
        1 ttaatgtgtg tcatacactc agtggcgtaa tcgttgacaa tatggtgcat gtaatcgtaa
       61 gcgtccagcg aaatccatct ggtcataatc tgaagcatcc gacaaagtgg tgtccaccaa
      121 ataagtgatc tgcgcccaat atcctgagcc ggaactttga caaaggccaa cgaaaaatgg
      181 gagcggatgt tacagagttc tcggtacagg ggaaaagtgg tacctgtcgc cagttctccg
      241 atctgtttaa ccgggaaatt atctcctaca gcttgtcaga aaggtcggtg atggagcatc
      301 gttaatacga tgcattcaca gtgcccggcc agaggatgcc ccgtcgctgc atatggatca
      361 gggttggcaa tatcgaatcg caggctatag gcaaagttaa agccccatgg agtagcacaa
      421 aatatgccgc acaaaggaaa cggtctgaat aacgcagtga tgaagaactt cttcagcaca
      481 ctgctaaaac gcatagtgat cgagcgctga ttctggcgaa caactgaact aatacatcag
      541 aatctgcatt atgttaaata aatatgaaaa gatggtttaa ataccccgtt acttttgact
      601 tacactatac ggtatcgcat cgtttaatat tcgcaccggc cagattttta tttctattag
      661 ttgtcacaat actgaatgcg tacgaccaca gtattctggc tcctgtgtgg ttatgcttta
      721 attctgcgtt ccgggcagat aagcagttgc ttgcaggaat ccttcttgtg ttaatgtcag
      781 ttcccctttt taccagtgct gatttccaca ttccgtccaa cagagcttat agcctttccc
      841 tggattatag cattgtccgg ctgaagttct ttttgaataa taatagaagc actgctggca
      901 gatccagtcc gtttttcata acccactgta ctgataacca taatctaatc agtagaaatt
      961 gagtcgaaaa taagcactac tccatacagg ataattagag gtcagtttga ttattcacaa
     1021 ttcatcatca gcattttcta tttctgacga aatcaacatg aaaataacca tatatgataa
     1081 ttattataat aacggcttta attggaatac atatattaca acgtattata tataattggt
     1141 attctgggaa ctatattctc aaaatacagt agaaacgagg tatgtttctg gtggaaagga
     1201 cagtgggatt aaaaagtaag aattgataaa aaaaacgcca gcaacacata gtgccggcgg
     1261 agggaatacc ccatggagaa aatgtgatgc ctagagcatc atgaatatat aattaaaaat
     1321 agttagcgtt gtcaatactt tcacaaaaat aattttcgta gtatagaaag atatttttat
     1381 gcatgaccta cctgaatttg ctccgggtag aggttataaa taaaaattga atcacgacaa
     1441 acacaatatt catagtatgg cgatgcctac gccagcaaga atagcgccaa taatattggg
     1501 aatataatag acaccagacg cacaggcatc tcactcctta acaaacaata atcaggatat
     1561 ttacttttac ccagctaact gtttacaccc aaagtgcaca caattaacca ttcaataaca
     1621 aatggcaata tccatagcca tacgacttta ccttgtaatg ttcggtattt ctttataatt
//
LOCUS       DAJVQH010000266         1653 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJVQH010000266 DAJVQH010000000
VERSION     DAJVQH010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.271"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..132
                     /gene="kil"
                     /locus_tag="OQM87_004219"
     CDS             <1..132
                     /gene="kil"
                     /locus_tag="OQM87_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCS1510093.1"
                     /translation="ASANNIKKRKLYIRSLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            132..302
                     /locus_tag="OQM87_004220"
     CDS             132..302
                     /locus_tag="OQM87_004220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510094.1"
                     /translation="MTKKIKCAYHLYNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            377..652
                     /locus_tag="OQM87_004221"
     CDS             377..652
                     /locus_tag="OQM87_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510095.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            752..>1653
                     /locus_tag="OQM87_004222"
     CDS             752..>1653
                     /locus_tag="OQM87_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCS1510096.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHA"
BASE COUNT          522 a          370 c          372 g          389 t
ORIGIN      
        1 gcctcggcaa acaatattaa aaagcgaaaa ctttatattc gtagcctgac tacaaaaaca
       61 tgcatttctg actgcatgat taaagtcttc ctcggtcgtg atggtttacc tgtaaaggcg
      121 gagtcatggt aatgactaag aaaataaaat gtgcttatca cctttacaat aaagaaattg
      181 aagaaagcaa aatcattaca agaccacttc atttcatgcg tggagttata ccaacgacgg
      241 aaatgaaaaa atattgtagt gaaatctgtg ccgaaaaaga ccagatggca cacgaacttt
      301 aattaactga ctatccgaaa ctgaatttat gccagcaatg gcagggattc gctcaacctt
      361 aattaaggag aaaaacatga ttaccagtta tgaagccact gttgtaacta ctgatgacat
      421 tgttcacgag gttaatctgg aaggaaagcg tattggctac gtgattaaga cagaaaataa
      481 agaaacccca ttcactgtgg ttgatatcga cggtccatca ggcaacgtta aaacacttaa
      541 cgatggtgta aaaaaaatgt gtctggtgca cataggaaag aatctgcccg cagaaaaaaa
      601 agccgaattt ctggcaactc tgattgcaat gaaattaaaa ggtgaaatct gaaaaaaaga
      661 aagcctgcac actgtgcagg cctgagtgaa gaacctggga catttattca tcactcgcag
      721 taattttaat ctgagttgag gttaaaaaac aatgaacacc gataaacaag tttacccact
      781 gtattacgaa gcaaaaaatg acaaagtaag aaaacgtctc ggtattaaag gcggttttta
      841 ctgggctgaa gcgaaaaaat tatccattgc catctcccgt ggtgctgttg cgattgacga
      901 tgcaggctac gatgaagatg actttaaaaa acctgttcgc gtcaatttgc ccgttgttga
      961 tgacctcccg ccagaaggcg tatttgatac ggaattctgc aaccgttacg aaaaaggcgg
     1021 ggaagatggc atcacaatgg tatttatcgc atcttctccc tctgttcagg acaaaccggc
     1081 cagcactgac aataccaacg tcaacggcga agacatgaca gaaattgagg agaacatgct
     1141 cctgccagtt tccggtcagg aactgcccat tcgctggctt gcgcaacacg gcagcgaaaa
     1201 accggtaacg cacgtttcac gcgacgaact ccaggcatta cacattgctc gggatgaaga
     1261 actaccagct gttactgccc tagctgtttc ccacaaaacc agcctgctcg atccgctgga
     1321 aattcgcgat ctccacaaac tggtgcgtga tactgacaga gttttcccta atccaggcaa
     1381 ttcaagcctg gggctgatga ctgctttttt cgaagcatac atggacgcag actacaccga
     1441 tcgcggtctg ctgacaaaag agtggatgaa aggaaatcgt gtttcacgca tcacgcgcac
     1501 ggcttccggc gctaatgctg gcggcgggaa cctcaccgat cgcggcgaag gtttcgttca
     1561 cgatctgacg tcactggcgc gcgacgtagc cactggcgta ctggctcgtt caatggacgt
     1621 ggacatttat aaccttcatc cggcacacgc taa
//
LOCUS       DAJVQH010000267         1605 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJVQH010000267 DAJVQH010000000
VERSION     DAJVQH010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.272"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..695)
                     /locus_tag="OQM87_004223"
     CDS             complement(<1..695)
                     /locus_tag="OQM87_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1510097.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
     gene            complement(1011..>1605)
                     /locus_tag="OQM87_004224"
     CDS             complement(1011..>1605)
                     /locus_tag="OQM87_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1510098.1"
                     /translation="WWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHLDIGVGTGFYLT
                     HVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALHGQFDSISMFYL
                     LHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFS
                     NTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          468 a          297 c          338 g          502 t
ORIGIN      
        1 tgggcggttt gttgtaacaa agtttgcatt attctgggag atagttgatt ttctctaaga
       61 ttgacaacgc tatcagaact gaggccaagt atgctttccg gaattgccgt gatccggttc
      121 tgattaagcc agaatattct tataatatga tcatctcttt ctggaaactc tggtattact
      181 tccaaagcat ttctggctgc atcaagtaat tccaatgaca ttggtaacct cggaagtaca
      241 gacaggtgat tgtcacttac attaatatat tctagtgacg caggtaattc aggaagtgcg
      301 aacaaatggt tatcactcac atttatatat tccagcgaca ctggtaattc aggaagtgca
      361 gataattgat tactgcttgc attcagctct ttcaatgccc ttggtagctc ggggagcatt
      421 gatagttggt tattgcttac attaatttca tcaagattgt caggcaatcg tggtagagat
      481 ctgagaccta aacaagataa gtccaacgat gtttcactgt tctccagaca taattggagc
      541 cgggtaaaag cagtttccct gttttctccg gaaatgctgt ttttagtcca ttcaacccat
      601 tcggagagat aattattgtg aacattgtcg attgatgtag ttctgtaaaa agagacgttt
      661 ccagtggata gggggggatt atttacagga agcataataa cctcgcagaa ggatatcctg
      721 ataaaatgtg atactgggga ataataagct gaaataaaat gtttcaggat tgaaatttat
      781 tgttttcatt ttaaggaagt gatgttgatt taactgtaaa aaaacgatac tattttctta
      841 gatggtaaat gtctcgcctg tgctatattt tgtttttcgg ttagttaaat atcgtacgtt
      901 catatttgaa cgttctgccg gaatgcatta tcaatagagg taaagtcgca accccaaatc
      961 gtaaaggaaa ccgtagcacg tcttaagcaa gaacgtgctg cggttggatg ctattttttc
     1021 cctgaagcgg aaaacattac tacagtacct tgaaccttgg ttttaacatt ctcgaaatgc
     1081 tctgagagta tatgtgttaa gccttcttcg gaatcttttg tgtttgaaaa gatgcctttc
     1141 tgattgtaaa tgcgcatcag tttttgaccg aagctattgt gcacaactcc atcgccaaga
     1201 attgtggctc cgtatagagt tccatcatca gttaaggcct gcgccgcatt gcgtattaca
     1261 cagctttttg tagatatatt tccaggcagg cagtgaagaa ggtaaaacat ggaaatggaa
     1321 tcaaattgac catgtaacgc cgcaggataa ggttcaaaaa catcatggct aattttatgt
     1381 ttaatttttg attccccagc ccttgtagat gccgcgttca ggctagcttc gttcaaatcc
     1441 attaaagata tcagactact ctcaggtacg tgagtaaggt aaaacccagt tccaacacca
     1501 atatccagat ggttgttacc taaatgttcc agaaagtgtg gaagaaggtg ttcctttgta
     1561 ggacatcccc atgcaagccg atttgatact cccaaaaccc accag
//
LOCUS       DAJVQH010000268         1565 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJVQH010000268 DAJVQH010000000
VERSION     DAJVQH010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.273"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..92)
                     /gene="yjbD"
                     /locus_tag="OQM87_004225"
     CDS             complement(<1..92)
                     /gene="yjbD"
                     /locus_tag="OQM87_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCS1510099.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHG"
     gene            complement(345..>1565)
                     /gene="lysC"
                     /locus_tag="OQM87_004226"
     CDS             complement(345..>1565)
                     /gene="lysC"
                     /locus_tag="OQM87_004226"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCS1510100.1"
                     /translation="ITNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREE
                     IERLLENITVLAEAAALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKV
                     MRTNDRFGRAEPDVAALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDY
                     TAALLAEALHASRVDIWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHP
                     ATLLPAVRSDIPVFVGSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNML
                     HSRGFLAEVFGILARHNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSAL
                     CRVEVEEGLALVALIGNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGED
                     AEQVVQKLHSNLFE"
BASE COUNT          379 a          416 c          400 g          370 t
ORIGIN      
        1 ccatgcgcaa tccgcgcccg atccagcagt gttttaagtt cacgctgctc gcgttcagtc
       61 atctcttttt gggtaatacg ggggagtgcc atgatggtgc cctcagtgag ccagagagtt
      121 cagtgtacgg gaattcgttt gcgagcagaa ctgataatat tcattttgcc ttctcttaac
      181 tatttatctc tttcgataat taattagaga tgcagataaa aaaattaaag gcaattattc
      241 tccgataaag acgttggtta acagagtaca ggctcgttta ttgatgagca tagtgacaag
      301 aaaatcaata cggcccgaaa tatagcttcc aggccataca gtatttactc aaacaaatta
      361 ctatgcagtt tttgcaccac ctgctcggca tcttcgccgg gcaccaggaa gcacaggtta
      421 tggctggatg cgccgtaaca aatcatgcga atgttgaacg gttccagtac gccgaatacc
      481 tctttgccaa cgccgcaggc ttttgacagg tcattgccaa tcaacgcgac cagcgccaga
      541 ccttcttcca cctccacccg gcacagtgcg gaaagctcca tcagcagaga ttgcgtcagc
      601 aacgtatcgc cagtggaggt tgaaccggtg gtatcaaggg ttaatgccac gctcacttct
      661 gacgtggtga ttaagtctac cgaaatatta tgccgcgcga ggatgccgaa aacttccgcg
      721 aggaagccgc gagaatgcag catattcagg ctgtgcaaag tgagcagagt ctgattgcga
      781 cgaagcgcca gcgcgcggaa cagcggcgga ttttcagttt tattgcacac cagcgtacca
      841 cctgcgcgtg ggtctttgct ggagccgaca aagaccggga tatcgctgcg tactgcgggt
      901 agcaacgttg ccggatgcag tacttttgca ccaaaagttg ccatctctgc cgcttctgca
      961 aacgcgattt catcaatgcg ttttgctgcg gaaactacgc gtggatcggt ggtgtagatg
     1021 cccgggacgt cggtccagat atcaacacga gatgcgtgta aagcctccgc cagcaaggct
     1081 gccgtataat cgctgcctcc acggccaagc gtcgttgtac gacctttatt ttcgctaccg
     1141 ataaatccct gggtgatcac taagccttca ttgaggcgtg ggagcagctg cagcgcggcc
     1201 agttccgcca gcgcggctac atctggctct gcacgaccaa atcggtcgtt ggtacgcatc
     1261 actttacgta catcaaacca ctgtgcctga acatcgcgtt cgcgcaggat ttcgacaaac
     1321 agcagggtcg acatcagctc gccgtggctg accagctcat ctgtcagcgc cggagacgtt
     1381 gccagcgccg ccgcttctgc cagaacagta atgttctcca gcagacgttc aatctcttca
     1441 cggataacgt tcgggtaacg cagacgttcc agaatggcaa actggatgtt gcgaatagcg
     1501 tcgagttttt cgaatcgctc gccaggttcc agcccttccg ctaaagcgac cagcagatta
     1561 gtgat
//
LOCUS       DAJVQH010000269         1565 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJVQH010000269 DAJVQH010000000
VERSION     DAJVQH010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.274"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..278)
                     /locus_tag="OQM87_004227"
     CDS             complement(<1..278)
                     /locus_tag="OQM87_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1510101.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPS"
     gene            416..1336
                     /locus_tag="OQM87_004228"
     CDS             416..1336
                     /locus_tag="OQM87_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1510102.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1336..>1565
                     /locus_tag="OQM87_004229"
     CDS             1336..>1565
                     /locus_tag="OQM87_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1510103.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGG"
BASE COUNT          434 a          370 c          379 g          382 t
ORIGIN      
        1 gatgggcggt tctgcagatc gctcagggat gtcagggcag aatctatgtc atgcaatata
       61 gggccaagaa cagacaaaag atgctcgccc gcttcagtgg gtacaacgct gcgcgtggtt
      121 cgcgtcagaa gccgcaatcc caatcgttct tctatacggc gcactatctg gcttaaagct
      181 gactgcacca tgctcaggcg ggctgctgcg cgagtgaagc tacgctcctc tgcaacgacg
      241 acaaacgcca tcagatcagc gatttcttca cgcttcattc agtatctcca tacaatctat
      301 taatcattta aatgataggt ctaatcatga tttagggtct aatcaactta atgatcaata
      361 tctatagtga tatcaccaac cgtcaaaacg aaccgacaac tccatgagga actgaatgat
      421 gaataataaa gtcagcttca ctaacagcaa taatccaacc atctctttgt ctgcagtgat
      481 ctatttccct ccaaaatttg atgaaacccg tcagtatccg gcaattgtgg tatctcaccc
      541 aggtgggggc gttaaagagc aaacggccgg aacctatgcc gaaaagctgg cggaaaaggg
      601 atttgtcact attgcttatg acgcatctta tcaaggtgaa agtggcggcg agccgcgtca
      661 gttagaaaac ccatacattc gtactgaaga cgtcagtgcc gtgattgatt acctgacaac
      721 gctttcttat gtcgacaata cccgaattgg tgcgatggga atttgtgccg gtgcaggata
      781 tacagccaat gctgcaattc aggatcgccg catcaaggcc atcggtaccg ttagtgcggt
      841 caatattggt tcgatgttcc gcaatggttg ggaaaacaat gtgaaatcta ttgatgctct
      901 gccacacatt gaagctggct caaacgcccg aaccagcgat attagcagcg aagaatacgc
      961 cattatgcca ctggctccaa tgaaggaggc cgatgcgccg aacgaagaac tgcgtcaggc
     1021 ctgggagtat taccacacac ctcgtgcgca gtatccaaca gcaccgggtt acgctacttt
     1081 gcgcagcctt aaccagatta ttacctatga tgcttaccat atggcggaag tgtacctgac
     1141 tcagccgatg cagattgtgg cgggcagcca ggcagggagc aaatggatga gtgacgacct
     1201 gtacgatcgg gcatcaagcc aggataaacg ctatcacatt gttgaaggcg caaaccatat
     1261 ggatctttat gacggcaaag cctatgtggc tgaagcaatt tcagtattag ctccgttctt
     1321 tgaggaaact ctgtaatgaa acatattcaa atccgcaata gtgatatggc ctggcatatt
     1381 gctgccaata ttcagtttcc accaaatttt gatgagtcaa aacagtatcc ggcgattatc
     1441 agcgtccatc cttttggcag ttgtaaggag cagacgtctg gcaatatcta tggcaaggca
     1501 ttggctgaaa aaggctatgt cgttctcgct tatgacgcca gtttccaggg agaatctggt
     1561 gggga
//
LOCUS       DAJVQH010000270         1512 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJVQH010000270 DAJVQH010000000
VERSION     DAJVQH010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.276"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..1071)
                     /locus_tag="OQM87_004230"
     CDS             complement(<1..1071)
                     /locus_tag="OQM87_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCS1510104.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLS"
BASE COUNT          454 a          242 c          326 g          490 t
ORIGIN      
        1 ggaaaggcgg tcaaggaacg cggagaatgc ccgggaattc tcctcggtgg aaaaagaagt
       61 ccattgtgta gaaacctccc gacgatctgc ttcaggaaac caggctgcca cagtttcggg
      121 aagagggggg atctgaaagt tctgcccccc ttcagtgatg actctgatat ggcgcgaatg
      181 taaattttgc ggcaacatct ggcgtaaaaa cagtctgttt tggtctgaca gggaaatacg
      241 aaactgaggc ccgttaaaat ttggaaccga agtttgctgt attaagaata gcagagtttg
      301 atctgataag gggttatttg caatctcaat agttatatga ggatctaaac gaaaaagatt
      361 tgtcgggatt gtagttattt ggttgttttc aaaataaaaa aatcttctca tgataatatt
      421 ttcttggatg tttggtaagt tgcttaatct attgctatat gcaaaaagaa gttgcagtct
      481 cctaggtaaa gggggcagtt cagtaagatg gttgtgcgca accttaagta tcttaagatt
      541 agaaggcaga gatggaagag tttgcagttc gttgttccta acctcaagtt tttttaaatt
      601 caagggtaat gaaggcagtg tggataacct attatactct gcgcttagtg atttgatggt
      661 gtctggtaat aacggcagat tctctaattg attttcggat acatccaaag ttattagata
      721 atgtggtaat gcagatatat gtgttaagaa attatctctt gcaataagtt ctttaatatt
      781 ttcaggtaaa tcagtaagga tggttaattt attacagctc acatcaagct ttttaatgca
      841 agcagggagt acaggtaacg aggaaagcgc caaggctcgc aggtccagtt tatgcccctt
      901 agaggtaaga catgcacgaa gtcttcttac cgcctcagta cgctgttctt ccggtaaccc
      961 ctgtttctcc caatcatccc attcggacaa ataatggttg ggggatgtaa ttgtgctgat
     1021 atgtggaaat aaacaggatg aaatatttct gagcatatta atttctcgca tatggatatg
     1081 gaattagatt gtgaataaat tgtatttaat gtatctctcc taatgaggag ggtgtttcag
     1141 aatattcgcc ataagggaat gagtgttata tcatgctttc taccctggta agtatatttg
     1201 cttttttcgt atatttggtt catctttttt tgatgtttta catattcaca atgcagagga
     1261 tttataccag aagacattag tcttgagtaa gatactacaa catctccaac tattttagcc
     1321 agcctgacta cttgacttgg taatatttgt tgctaataat catgtataaa ataagcttat
     1381 atacatagta acttcagcaa aggatttagt aatgatccaa atcatatctc aatgtactgt
     1441 ttttttcttt aaaaaaacag tacattctat tttataatga ctgtgtattc ccatggaagg
     1501 agatcaccca at
//
LOCUS       DAJVQH010000271         1511 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJVQH010000271 DAJVQH010000000
VERSION     DAJVQH010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.277"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..426
                     /locus_tag="OQM87_004231"
     CDS             <1..426
                     /locus_tag="OQM87_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1510105.1"
                     /translation="VRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEF
                     ADFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKM
                     IARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(580..1511)
                     /locus_tag="OQM87_004232"
                     /pseudo
     CDS             complement(580..1511)
                     /locus_tag="OQM87_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          358 a          382 c          368 g          403 t
ORIGIN      
        1 gtacggcgta aattcggggg gcgcttacat tcccgggagg atgggataaa accatttatt
       61 cagtcagtac gccagggata ctgggggtat taccttccag atcaggatca tggtcctgaa
      121 tacagtgaat ttgctgattt ttttgcgacc tataaagcga cattaccaat tattggacgt
      181 ctgatgaaca tcagtcaggc tatgattata ccgcttttcc cggtttatga tgaaaaaaaa
      241 catttcctga ctattgaagt tcggccacca atggatgcat gcattgccag cgcggacaat
      301 aaaatgattg cccgacaaat gaacaaaaca gtggaaattt tggtggggtc acatccggaa
      361 cagtatatct gggttttaaa attgttaaaa acgcgcaaat caaacgaagc ggacccgtac
      421 ccttgaaatt ataaagtaca ggtatttcca ctagttgttt cttacaggtt accaatcgaa
      481 acacatcccc cttccgtgtg ttgcccgttt ggcacggagg actgtgtaga tgcagttcgt
      541 taaatttaat ttatgcctaa atagctgcgc ttaataccgc tacacttttg ccagcccatg
      601 tttgcctccg gggaatgggc tgacggtttc cataaaatgg cgaacttttt tcaacagttg
      661 ccacattgag cggcactgat gattacgcgt tattgtgtcg tgaagtgcct gccatagccg
      721 ttcaacatga ttcacccatg gcgagtaaac cggctgataa atgcccctga acttcgggtt
      781 ctccttcagc cagctctgtg tttcccggct tttgtggata atgtagttgt ccacgatcag
      841 cgtgatggtt ttcgcccgac ggtatgtcgc tttaagccgc ttcagcaggc tgatgtaaca
      901 gcgccgaact tttgctgttg ccgcccacat agctgacttt acctgtcccg ctgtgcagcg
      961 ctccggccag ataatatttt tcattctgtc ccggcgtgac cacccgtttt tgctgtccgc
     1021 gcagttgcca gtccgcaccg attttgggat gaagatggat atccacttca tcttcataaa
     1081 agaccggatg ctctgcgctg cattcgtcca gtgctttatg gattgctgcc atcttttcat
     1141 ctttatgcgg gtcacggata cgcagagttg gcgcagccct tcgccacaca atccccgcag
     1201 acggcaacca gcggcgaacg gttccggcat ttaactggca accggttatc tcattgattt
     1261 ttattgccag cagttctgta ctccagcgtg aacgctggta gccaaagtcg ccgggagaat
     1321 gttttaccag ctcacgtaac agtgtgcaga tatgctcaaa cggccagcga cgggcacgcc
     1381 cggcaggtaa tgatttcagt ccctcaacac ccgactgcgt gaaccagtta atccagcgtc
     1441 caacagagga gcgggcgcag cagagcgttc tggcaacgtc gctgacacgg tcgccccggt
     1501 gcagctgagg t
//
LOCUS       DAJVQH010000272         1508 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJVQH010000272 DAJVQH010000000
VERSION     DAJVQH010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.278"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(156..335)
                     /locus_tag="OQM87_004233"
     CDS             complement(156..335)
                     /locus_tag="OQM87_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510106.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGISAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            424..696
                     /locus_tag="OQM87_004234"
     CDS             424..696
                     /locus_tag="OQM87_004234"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510107.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASGSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
BASE COUNT          380 a          304 c          349 g          475 t
ORIGIN      
        1 gttaggcgtt catgtggcag cgcggcacgt ccccatttag tggcaaactc ccgttggcgt
       61 gcctgtaaca gttgctccag cctttttatt ttgttctcat tcatccggtt ttcctcgtat
      121 gctgcatcac accactgtgc taatgaaaaa tgtaatcagt gtggtggacg tgttttatcc
      181 gcattggaat tgtcgtcaac taaaggagat gaggcccgtt tgtatcgcaa tgggtctatg
      241 gcgcttatgc ccgtttgctg ttcgataatg gcttctctga gcatacggtg ttgcagttct
      301 cgtaaaaact ttgcgtcttc tggtgataaa tccattgtta ttactcgtaa agcaatgcgc
      361 tgtagtgcct tacgctaccg cgctgatggt tcttatccgt tttattgaat cggagaacaa
      421 actgtgagct atcagcctgg tggtctggtt ttgcctgatt taagccgccg tgggtatttg
      481 tccaagaatg cttcaggctc caatcgtttt ggcctggtga atggagatgg gttaagcaaa
      541 gactctcgtt gctggtgtac tttttcgcgc tggcgctcaa gcatggataa tgcgattgcc
      601 tgtagttctt cgttagtcat tgtctgcacc tggaaagcaa tttaccggag tacctcacac
      661 tacggcaaaa atgttttatt gttgcaactt gcgtaattat tcaggtcagt ttggaacgca
      721 ctgtataaac ataaataacc tgatactgtt gtgtgttttc aaagaaaaca caggtatttt
      781 tcacataaac cttccccttt tcatattcga ccacttaatc aaaaaacggt tttatacttg
      841 ttgcccccac tggggttaaa tgtgagacat gtcgcacttt gtgccatgtg tccttgctga
      901 ttttaggcgc ggttcattca ttctttctgt tggcataaag tatggtatcg gttgggcgaa
      961 tgaaagggac tcgggtcatg gtcagacaat acctgaggcg ttaattcgtt gaaagagatt
     1021 gtgaatgggg gaataactga gtcttaactg ctgtgcgata gcccggacag ataattgctc
     1081 gtcgaatctg agacgtaatg attctggcaa ataagtgcga aagccggtga tatctagtgt
     1141 tgtttgagag ttcatcgttc gcgtccatta atacagatgg acgcgattgt tgccagacag
     1201 gataattttc acaagacatc gcagatagat gtacttacga taatttttta ttttatacaa
     1261 cagatgccca cgaaaagaat tattttcaat agacacctgc attacacaaa gtagatacat
     1321 acgcagtcag agtacactta ctttttatta tttcctgcaa tggcgattac ggctattgac
     1381 agtaatgaag ttattagtga accaagacct aaagtagtcc cgggaccaac accgttgtcg
     1441 cttacggtta ctgacaggaa actgattgta ccagcgctta ttgtttgagt aaaacctgta
     1501 atagatgc
//
LOCUS       DAJVQH010000273         1477 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJVQH010000273 DAJVQH010000000
VERSION     DAJVQH010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.279"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..336
                     /locus_tag="OQM87_004235"
     CDS             <1..336
                     /locus_tag="OQM87_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HCS1510108.1"
                     /translation="MPENFSDTRWAADIHITPDGRHLYACDRTATLITVFSVSEDGSV
                     LSKEGFQPTETQPRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQG
                     PMWVVVNAH"
     gene            complement(377..1330)
                     /locus_tag="OQM87_004236"
                     /pseudo
     CDS             complement(377..1330)
                     /locus_tag="OQM87_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT          395 a          350 c          327 g          405 t
ORIGIN      
        1 atgccggaaa acttctccga cacccgttgg gcggctgata ttcatatcac cccggatggt
       61 cgccatttat acgcctgcga ccgtaccgcc actctgatta ccgttttcag cgtttcggaa
      121 gatggcagcg tgttgagtaa agaaggcttc cagccaacgg aaacccagcc gcgcggcttc
      181 aatgttgatt acagcggcaa gtatctgatt gccgctgggc aaaaatctca ccacatctcg
      241 gtatacgaaa ttgttggcga gcaggggcta ctgcatgaaa aaggccgcta tgcggtcggg
      301 cagggaccaa tgtgggtggt ggttaacgca cactaaccgc tgatttaacc ggcgcagtct
      361 ctcctgcgcc ggtgtattaa cttatctcct gtaacgcatg tctctggcgt tcgacgatat
      421 tggtccacaa attgtcttta tcgtcagtcc acaaattaat cagcaaggca aaaaagcgtt
      481 cagctgccgg agaaagcacg gcatctttgc ggcgaataat ccccaatgtc cgacgtataa
      541 cgggttccac cagtgggata ccaataatcg aagaataggg cgcatggggc attgccagac
      601 caggaagcgc cgaaatcccc agtcctgcct ccactagtcc taatgacgtc gaaagatgac
      661 gcacttcgta aaaccagtcc agcttccagg gcttgtcggc tagctgttgt tctatcaaca
      721 atcggttgcc gctggaggaa cgtacgccaa tcattttgta acccactaac tcctgccatt
      781 ctacaagcta ctttttggcc aatggatggt cacgccgaca ggccagcacg aatggctcgt
      841 tgaccagtgg ggtaaaatca atggatgaat ttgtgacgtt gttcatgttt atgccaaagt
      901 ctgattcgtt acacagcacc gattccatgc aattattcgt gccttgttcc agaatccgca
      961 ctttaatatt gggatacagc tcattaaatt taccgattgc cagcggtaaa aaataaaata
     1021 ctgcggtcgg aatacacgct aatgtcacca taccacgatg ataagcattc atatcacgaa
     1081 tattaaaaag cgtttcatcg aatttcttta ttaattcccg cgcctccggg agcaaccttt
     1141 ttcctgcttt tgttaacgta accttgcgag ttgtacgctc aaatagttga atatgtaaat
     1201 cttcctccat cttttttatt cttcgcgtta atgcaggttg cgtaatattg agtaatttag
     1261 cggcattgtt aaatgaactg gactctgcca gtatgacaaa tgccttcata cttgataatt
     1321 catgcttcat tattactccg gaaaatggaa gcgacgattt tggggggctg gtcgttaaaa
     1381 attttaactg catttagcca acttaaatta atgaaaaaat gttattaatc gttgagctaa
     1441 agtcattaga gatgctttgt ccttaatgta accatat
//
LOCUS       DAJVQH010000274         1471 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJVQH010000274 DAJVQH010000000
VERSION     DAJVQH010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.280"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(185..337)
                     /locus_tag="OQM87_004237"
     CDS             complement(185..337)
                     /locus_tag="OQM87_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510109.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            355..546
                     /locus_tag="OQM87_004238"
     CDS             355..546
                     /locus_tag="OQM87_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCS1510110.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWLHH
                     QSKKEAQQSTLSVESPVQR"
     gene            857..1375
                     /locus_tag="OQM87_004239"
     CDS             857..1375
                     /locus_tag="OQM87_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1510111.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT          430 a          321 c          277 g          443 t
ORIGIN      
        1 catagatgct cagcagaatc ccccacatcc tgaaggaggt gtattcagac aggcatccca
       61 cctgacttcg aatgatgatt attcatcact atagagagca ttgattctaa gtgtcatatg
      121 aaagttccaa ttgatatata tcaaacaaaa taaccctgat taatgaatta ttacgtttat
      181 catgttaatt catcattatt acatcatcat tgtaaataat taaattaact tccataacat
      241 taaaatatgt atccactgac gcttttttac ataacgaaga attgaccatt ttgtcctgtt
      301 gtgccttaat gtaagtaccg tccacagcgt gggacatact tcaaggaacc ttttgtgagt
      361 caggcaacta gtatgcgaaa acgacaccga tttaacagtc gcatgacccg tatcgtactg
      421 ctcatcagct ttatcttctt ctttggccgt tttatctact cgtccgtcgg tgcctggctg
      481 caccatcaga gcaaaaaaga agctcagcaa tccacactct ccgtcgaatc accggtacaa
      541 cgttagcggt taccttctcc actttcacag aacataacgg cacttcgctg tcggatgctt
      601 ttgctctttg ggattatcaa agcggcagat attctttcat cttaaatttt acgtctttgt
      661 cctgactgat gtttatcctg tttggctgcg aaataaatat aaaattaata tatatgttgt
      721 aatgatatat ttttataaat tattccctgc gtgaatttta ataaatttaa tctatccctt
      781 tatacgcaat acatttactt tcctcttttg atgatcttaa atgtcttatt tttcgtaatg
      841 tgtataacaa ggaatagtga tgaaatttaa aaaatgtctt ctgcctgtgg caatgttagc
      901 gtcattcact ctggcaggat gccagtcaaa tgctgacgat catgccgccg atgtttatca
      961 aaccgatcaa ctgaatacca aacaagaaac taaaaccgtt aatattattt ccattcttcc
     1021 cgcaaaagtt gccgtagaca actcccaaaa taaacggaac gcacaagcct tcggcgcgct
     1081 tattggcgca gtcgctggcg gtgttatcgg ccacaacgtc ggttctggca gcaattccgg
     1141 aacgacggca ggggcagttg gcggcggagc tgtaggcgcg gcagcgggtt ctatggtgaa
     1201 tgataaaacc ttagtggaag gtgtttcttt aacatataag gaaggcacca aagtgtatac
     1261 ctccacccag gagggtaaag agtgccagtt tacgacaggt ttagccgttg ttattaccac
     1321 aacgtataac gaaacgcgta ttcagccaaa taccaaatgt cctgaaaaga gctaataatc
     1381 aggaggagtc atgaagaaag tttttctttg cgccatctta gcctccttaa gctatccggc
     1441 tatagcctca tcattgcagg atcaactctc t
//
LOCUS       DAJVQH010000275         1444 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJVQH010000275 DAJVQH010000000
VERSION     DAJVQH010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.281"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..155)
                     /locus_tag="OQM87_004240"
     CDS             complement(<1..155)
                     /locus_tag="OQM87_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1510112.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGR"
     gene            complement(220..1170)
                     /gene="virK"
                     /locus_tag="OQM87_004241"
     CDS             complement(220..1170)
                     /gene="virK"
                     /locus_tag="OQM87_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCS1510113.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            complement(1175..>1444)
                     /locus_tag="OQM87_004242"
     CDS             complement(1175..>1444)
                     /locus_tag="OQM87_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCS1510114.1"
                     /translation="FGMVLAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPASNKHAW
                     MCALNDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAKQK"
BASE COUNT          449 a          312 c          272 g          411 t
ORIGIN      
        1 caacgcccta tttttgccaa gacaggatct ctgaatgaag ggggaaacat tgaaatacct
       61 gcaatcattc ccaaaaggaa ccatgtagac cagtaaacag gggaaaggta atttttctta
      121 aattcaggaa taaactcaga tttatatttt ttcattattt agtgcgaatg cctctaccaa
      181 aattatcatt actgttttat tcagagaata catcaaacct taatttaaat tctgatgttc
      241 tgagtgtaca ggatatgcat ccaaagagcg gatagtagtt tcatggatat aatccagcaa
      301 agcgtatcgt tttcggtact cagagcgttt tttactggct atatcgctta gcggctttcg
      361 aatcacctgg gttggtaatc tgtaccagtc accacaggtt tttcctgcta cagactccca
      421 gaaatcacta tagaccgcaa caaaggtttt gcgtttctga taccagtatc tcaattgccg
      481 gaaaacatga ctgtgctcac tgactgccag acactcagag atattgcaag ctttcatcaa
      541 cgcacaaaat gcttcaaaaa taatccgttt tgggaaaatc ccataacagg cccgggttgc
      601 gcaccgaata acatcaggtc cggtattttt aggggctccc tgtaatccgc caataaaagc
      661 aatgtgtcca ttttgagtca gaatgactga aaatgttaac cgggcaataa ctgttttatt
      721 gtaaaacaat gacaacatca actctccttc ccggtcaaaa ccactgggag aacactggat
      781 atcaaaattc tctccatctt tcccgatgaa ttggactaat gataccgatt tttgtggaaa
      841 caaaaattta gaatattttt ctggcaaatt ttgcacaaag cgataatgct caagaatata
      901 ttgtccacgg gtccaggcgc gccccccttt atgcaaataa gggcgatgaa ttcttgctgg
      961 caacgtgggg tgtattgcca gaatatcctc aatgcacggc aaggaacgca gttcataata
     1021 atatcgaata ctgacaaccg gatttataga acatcgcaaa atgtacatga accgaaattt
     1081 tctgtgttcc cacttccccg gtagtgaatc tgaggaaaaa ataatccttt taaggaaacc
     1141 gataaatgat aagttactta tagaaaacat attgttactt ttgcttcgct aatgtgagta
     1201 tttttaacgc agttttatta atatcaaacc gctcttctat atcctgtttt gccaggcgag
     1261 ccatctgata aaaacgccca gggtcattaa aaaaatcatt cagggcgcac atccatgcgt
     1321 gcttattact tgctggtaac aatgtcccgg tctggttgtt ctgaataacc tcagggattc
     1381 caccaatctg agtggccact acaggcacag aaaatgccga tgcttctgcc agcaccatac
     1441 caaa
//
LOCUS       DAJVQH010000276         1415 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJVQH010000276 DAJVQH010000000
VERSION     DAJVQH010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.282"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..684)
                     /locus_tag="OQM87_004243"
     CDS             complement(<1..684)
                     /locus_tag="OQM87_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1510115.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKI"
     gene            781..>1415
                     /locus_tag="OQM87_004244"
     CDS             781..>1415
                     /locus_tag="OQM87_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1510116.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     KVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT          409 a          288 c          336 g          382 t
ORIGIN      
        1 aattttggtc aacgaatatc catcatcaag aagcgtcact gcagaaataa cgtgcatttg
       61 ctgaacccag tggcgaaatg ttaatcccgt ctctttaaca aataagcgca tgagtgtacg
      121 gctactgacg ttaattttat ccgctagatt atccagtgtt ggcagatacc cttggttgtt
      181 tagcaaattc tcacaaagct gctgtagccg ctcatcattt ggccaaggga gttcaagtga
      241 cattttcacc cccattttta tttcctgaag tatcagtgtc tcgagggctg aaaaacgtaa
      301 ctgttgttta tctgtggtac tttgcgacgg tggaagttgg ttaattgcaa ttagtaattc
      361 ccttaacagg ggtgaaatac ccgtcatgaa gcaaactgtt cccagcgttt cactttccgc
      421 ttctgaaacc agtacggttt ttaaaagtac gttgcctgtc gcaacaaact tatgcggcat
      481 ttgtggcggc agccaaagcg cgctgctggc atggactagc cagacgcttt ctccagccca
      541 tacacgaacg actccttcag ccgggtaaag caactgggca tagagatgtt tatgccaggg
      601 aactgtacct ccattcagat gttccgaatc cagtccgata atgtgatgcc ctggtgatgc
      661 tttgggttgc agtgtaattg tcatttacag taggtatttt gtcagaaatc atcaaataag
      721 ccatgttaaa ttttaaatat gacgtatgca agtttccgga tatatacggt gtaaagagtg
      781 atgataattt ctaaaaaaca gcttattggg gtggtagcga tcggtattct tttgactggc
      841 gttgtctttt ttatctggag ggtaagcaaa ggaagattta tacagacgac ggatgatgct
      901 tacattggtg gtaatatcac cacggtggca agtaaagttt ctggttatat ttctgctata
      961 gaggtgcgcg ataatcagag cgtaaaaaaa ggcgatatta tcctgcgtct cgatgatcgg
     1021 gattatcgtg caaatgtcgc caggcttgag gcaaaaataa aaagtagcaa agccaatctc
     1081 gaaggcattc aagccactat tacgatgcag cagtcgataa ttcaaagtgc cagtgaaaca
     1141 tggcaggcag taaaacatga ggagcaaaag cgcctgcgag atactgaacg ttatgaaaaa
     1201 ctcgcacaat cagcggcaat atcgcaacaa attattgata acgccaggtt tgactaccag
     1261 aaggtcgcag caaaggagcg caaagccgcg aacgatttcc tggtggagaa gcaacggcta
     1321 gcagttttat ctgctcagga agaaaacgta cgtgcgagta tcgaagaagt tcaggccgct
     1381 ttgactcagg cactactgga tctggaatat accct
//
LOCUS       DAJVQH010000277         1389 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJVQH010000277 DAJVQH010000000
VERSION     DAJVQH010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.283"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(1..572)
                     /locus_tag="OQM87_004245"
                     /pseudo
     CDS             complement(1..572)
                     /locus_tag="OQM87_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(620..1114)
                     /locus_tag="OQM87_004246"
     CDS             complement(620..1114)
                     /locus_tag="OQM87_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1510117.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGV
                     GIRIFNQSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTV
                     EYKK"
BASE COUNT          423 a          280 c          263 g          423 t
ORIGIN      
        1 atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc ttcccggcgc
       61 catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg tcgccagaac
      121 agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt gctcgctgat
      181 ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg caatgacaca
      241 cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg tgatactggc
      301 ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc tacccaagcc
      361 tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt atcagcatta
      421 ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt taatgctggt
      481 tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac tgttgtttta
      541 aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa gccaattgaa
      601 taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg
      661 ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatttcag
      721 ccttgccttc agcatcgata gcggtataat cagagtcgtt ataaagttta acctgcgaat
      781 tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac
      841 catcaaaacg aacggctgct tgagtgtacg ttgccgggca attttcaagt ttaatactga
      901 acttctgtgg cgacgccaga ctacctacac ctgaaaatgc atttttactg actttaccca
      961 gtgggacaat ttgatcggct gtatcggagg taacttcaca agtagtgtcg acaatttcac
     1021 cgacgaagtt aacaacacca tcttctgcca gtgcatgccc ggccagcatt agagatgcca
     1081 gagtgaatgc accgcctata atattacgct tcataaaaac tccattgata taatatatcc
     1141 tttaccatga atcatcactt atattaataa gtgtattttt gattcaaagg aatagtacac
     1201 caaagagacg gctttatcta aaaataatat taattacgca aaaataaaat atagatttat
     1261 tgtttaaata ttatttaaac agtaaaaaat caagtttgtt atcagacatt aatgataatt
     1321 cagaatatat ttatatagaa agatagatga atttattgat gtcagttata aaaaagggcc
     1381 accggtcgg
//
LOCUS       DAJVQH010000278         1355 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJVQH010000278 DAJVQH010000000
VERSION     DAJVQH010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.284"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            193..462
                     /locus_tag="OQM87_004247"
     CDS             193..462
                     /locus_tag="OQM87_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510118.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..685
                     /locus_tag="OQM87_004248"
                     /pseudo
     CDS             459..685
                     /locus_tag="OQM87_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            714..1304
                     /gene="ospG"
                     /locus_tag="OQM87_004249"
     CDS             714..1304
                     /gene="ospG"
                     /locus_tag="OQM87_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCS1510119.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          389 a          262 c          333 g          371 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaatctct tgtgattttt aaatatcgta acaaaggtat gacatgaaaa
      721 taacatctac cattattcaa acaccttttc catttgagaa taataattct catgctggca
      781 tagtaacgga gcccattctc ggtaagttaa taggtcaggg gtcgacagca gaaatctttg
      841 aagatgtgaa tgattcatct gctttgtata aaaagtatga tcttattggc aaccagtaca
      901 atgagattct ggaaatggct tggcaagaat ctgagctttt taatgctttt tatggcgatg
      961 aagcatccgt tgttatacag tatggcggag atgtgtacct ccgaatgctg cgcgtgcctg
     1021 ggactcccct tagtgacatt gatacagctg atatccctga taatatagag agcctttatc
     1081 tacagttgat atgtaaattg aatgagttga gtataatcca ttacgatctt aatacaggta
     1141 atatgctgta tgataaagaa agtgaaagtt tattcccaat agattttcgc aatatttatg
     1201 ctgaatatta cgctgcaacc aaaaaagata aagagattat cgaccgacga ttacaaatgc
     1261 gtacaaatga tttttattcg ttattaaaca ggaaatattt atagccgtat ttgttgatgc
     1321 tataagccag aggctcttct atgagaatat gggga
//
LOCUS       DAJVQH010000279         1347 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJVQH010000279 DAJVQH010000000
VERSION     DAJVQH010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.285"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            443..1192
                     /locus_tag="OQM87_004250"
     CDS             443..1192
                     /locus_tag="OQM87_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCS1510120.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          377 a          275 c          279 g          416 t
ORIGIN      
        1 gagtggtgga ttagcccggc aggtgggcgc tggctcctga gcaccataaa cagggcttta
       61 cccgtaaata ttggctcctt ctgatgccat tctatttcag gaaggagtgt ccgttaaact
      121 caggctacct caaaaagtta tcggtgcgtt tatcgaaaaa catatgttct actaattgga
      181 gtcattgtct gtaaaagcaa ctcaaaaata caagttgctt attccccttg ttggtaagaa
      241 cttaattcta actgtttgtt ctggatgttt taaacgtcct cctttttatg cttgttcata
      301 acggatattt aataggtaac ctttgtttta gcatcttctg atttatttaa attatgtgat
      361 agcgcacaca attttattat ccagtacttt atactcttaa taaaataatt ataaatattt
      421 atcctctata aggagatgtt atatgaaaag acagcttttt actcttagta ttgtcggggt
      481 attttcttta aatacctttg catcattccc tccgggaaat gatgtgacaa caaaacctga
      541 cctttactac ctgacaaacg ataatgctat tgacagtctg gcattattac cgcctccacc
      601 acaaatcgga agtattgcct tcctgaatga ccaggccatg tatgaaaagg ggcgtttatt
      661 gcgaaatact gaaagaggga agctggcagc tgaggatgcc aatctcagta gtggtggcgt
      721 ggcaaatgta ttttctgctg cttttggttc tccgataacg gctaaagatt caccagaatt
      781 gcataaatta ctgacaaata tgattgagga tgccggagac ctggcaacgc gttcagcaaa
      841 agaatattat atgcgtattc gaccttttgc tttttatggt gtttctacgt gtaatacaaa
      901 agaacaggat acattatcca gaaatggctc ttatccatca ggtcatacat cgattggttg
      961 ggcaacagca cttgtattgt ccgaaataaa tccggcacgc caggatacca ttctgaaacg
     1021 gggttatgaa ctgggggaca gcagggttat ttgcggttat cactggcaaa gtgatgttga
     1081 cgcagcacgt attgttggct ctgctattgt agcaacactg cactcaaatc ctgtgttcca
     1141 ggcacaatta caaaaagcga aagatgaatt cgctaacaat cagaaaaaat aatgctttta
     1201 gccagtacgc cttataaccc cgaccttcag ggctaatgcc agtcagttaa gcaactgact
     1261 ggctcttttt cagggctgtg gggtatttcc agggcctctc ctttaccact ctcgggaagg
     1321 ccctttccct tcttgtcggt aatttca
//
LOCUS       DAJVQH010000280         1343 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJVQH010000280 DAJVQH010000000
VERSION     DAJVQH010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.286"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            31..318
                     /locus_tag="OQM87_004251"
     CDS             31..318
                     /locus_tag="OQM87_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCS1510121.1"
                     /translation="MFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNT
                     VSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
     gene            321..866
                     /locus_tag="OQM87_004252"
     CDS             321..866
                     /locus_tag="OQM87_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1510122.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            890..>1343
                     /locus_tag="OQM87_004253"
     CDS             890..>1343
                     /locus_tag="OQM87_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1510123.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAA"
BASE COUNT          378 a          299 c          371 g          295 t
ORIGIN      
        1 ttcagccaga gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg
       61 ttaaattacg gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta
      121 cggtatggcc atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc
      181 gcaccttcgg gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg
      241 gataacgttg acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat
      301 aacgtagcga gtatttaaca atgttgcatt taaaaaatat tactgcgggt aatccgaaaa
      361 cagcagaaca atatcagatg acaaaacgat attcggtcac ctggcttttt tcagaagacg
      421 gaaaaaactg gtatgaagag ctgaagaatt tcgccagcga cacaataaaa atagcttaca
      481 ccggagatgg tcgcgtggtg tgggtcggta aggatgtgac aggcatcgag ccacgcaatg
      541 ccagcgttat cgaagttccg gatattaccg ctaaccgacg gattaccgcg ccgggttact
      601 ggttttaccg caatgatgaa tttgtctttg actacagact caaagcggaa gatgagcgtg
      661 atgcccttct ggctcaggtc agtgcccgga caggggaatg ggaagaagac ctgctgctgg
      721 ggttaatcag cgacgaagat aaagaaaagc tgaaagcctg tcgtatttac gcgaaatcgc
      781 tgcaggcgat ggatttcagc accatcactg ataaatcctc atacaacgcc attgaatggc
      841 ccgcctctcc ggaaggttct tcctgattta atttatcgcg agaaaaacaa tgtctgtagt
      901 gatatcaggt gcgctgactg atggcgcagg tatccccatg tccggatacc atattattct
      961 gaaatcccgg gtaaacaccc cggaagtggt gatgaacact gttgctgatg tgatgacagg
     1021 aaacgatggt gaatactgtt tccatgcgcg gactggaaaa tatggtgtgt atctgaaaca
     1081 ggactggcgc aacgagtaca acgttggcga cattgctgta tatgaggact caaagcccgg
     1141 cacgctgaat gactttctga ttgctcctga tgagggcgac ctgaaaccgg atgtcgtcaa
     1201 acgctttgag gaaatggtgg cgcaggcgca gcagagcgcc ggggccgcag ccggaaacgc
     1261 acagcagacg gcgcaggatg tggcggcagc cgcaggttat gcccgcgcag cagagcaggc
     1321 caaaaatgac attgatgctg cgc
//
LOCUS       DAJVQH010000281         1322 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJVQH010000281 DAJVQH010000000
VERSION     DAJVQH010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.287"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OQM87_004254"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OQM87_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCS1510124.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPIFTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          371 a          241 c          296 g          414 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta aaaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttatt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt agtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtcgtg agccgttttc agacaatcgc agggccagta cctcgtcagt
     1321 ca
//
LOCUS       DAJVQH010000282         1314 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJVQH010000282 DAJVQH010000000
VERSION     DAJVQH010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.288"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            214..>1314
                     /gene="amn"
                     /locus_tag="OQM87_004255"
     CDS             214..>1314
                     /gene="amn"
                     /locus_tag="OQM87_004255"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCS1510125.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVK"
BASE COUNT          320 a          344 c          296 g          354 t
ORIGIN      
        1 actcatcact tactttgccg ggaactggca tagcatcgcc atttatttgc tggctggatg
       61 cctgatttct gcaatgaccg ctttattgat gaaagacaat caacgctcgt gatagcctgc
      121 cgaagatcat ccgatcttcg ccttatactt ttgtttcaca ttcctgtgac atactatcgg
      181 atgtgcggta attgtatgga acaggagaca cacatgaata ataagggctc cggtctgacc
      241 ccagctcagg cactggataa actcgacgcg ctgtatgagc aatctgtagt tgctttacgc
      301 aacgccattg gcaaatatat tacaattggc gaattacctg atgaaaacgc ccgtaaacaa
      361 ggtctttttg tctatccatc actgaccgta acctgggacg gtagcacaac aaatcccccc
      421 aaaacgcgcg catttggtcg ttttactcac gcaggcagct acaccaccac gattactcgc
      481 cctactctct ttcgttcgta tctgaatgag caactaacgt tgctttatca ggattatggt
      541 gcgcatatct ctgtgcaacc ctcgcaacat gaaatccctt atccttatgt catcgatggc
      601 tctgaattga cacttgatcg ctcaatgagc gctgatttaa ctcgctactt cccgacaaca
      661 gaactggcgc aaatcggcga tgaaactgca gacggcattt atcatccaac ggaattctcc
      721 ccgctatcgc attttgatgc gcgccgcgtc gatttttccc tcgcacggtt gcgccattat
      781 accggtacgc cagtagaaca ttttcaaccg ttcgtcttgt ttaccaacta cacacgttat
      841 gtggatgaat tcgttcgttg gggatgcagc cagatcctcg atcctgatag tccctacatt
      901 gccctttctt gtgctggcgg gaactggatc actgccgaaa ccgaagcgcc agaagaagcc
      961 atttccgacc ttgcatggaa aaaacaccag atgccagcat ggcatttaat taccgctgat
     1021 ggtcagggta ttactctggt gaatattggc gtgggaccgt ccaatgctaa aaccatctgc
     1081 gatcatctgg cagtgctacg cccggatgtc tggttgatga ttggtcactg tggcggatta
     1141 cgtgaaagcc aggccattgg agattatgtc cttgcacacg cttatttacg cgatgaccac
     1201 gttcttgatg cggttctgcc gcccgatata cctattccga gcattgctga agtgcaacgt
     1261 gcgctttatg acgctaccaa gttagtcagc ggcaggcctg gtgaggaagt caaa
//
LOCUS       DAJVQH010000283         1311 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJVQH010000283 DAJVQH010000000
VERSION     DAJVQH010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.289"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..395)
                     /locus_tag="OQM87_004256"
     CDS             complement(<1..395)
                     /locus_tag="OQM87_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510126.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AVTDKSYLSLNPDKYPNYRFLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRD"
     gene            complement(1057..>1311)
                     /locus_tag="OQM87_004257"
     CDS             complement(1057..>1311)
                     /locus_tag="OQM87_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510127.1"
                     /translation="YNGADRGVFMMAKRFTALSFGCFRHDGENITVVLSRDGNTQSHD
                     LEILGKVFVVAEAATTVKMGTGSAAAVLPVVKRLSFTKTS"
BASE COUNT          388 a          275 c          287 g          361 t
ORIGIN      
        1 ctatccctca gccagacagc atcatagtgg gtttctgccg ctgttttgtc atcttcgaaa
       61 gtagaagcaa gaataaacgt tccccagggt ttctccgtca acgttaactt tcgtgtgttt
      121 tctgttaatg ttagcaatcc ttgaatttcg tctttggtat agtgactctt tatttcattt
      181 tttaatttgg ctgagtgaat cagaaaacga taattaggat acttatctgg atttaacgat
      241 aaataacttt tatctgttac tgcccccctg gaattaagaa tgttaaatac agattcgctt
      301 gtcacttctg cctctgtcgc ctggcatgtg ccgcacgaaa atataccagc gagcagaaga
      361 gttaatgcag atttcccttt cataaaataa tccatcaatc gtttgttaaa agcgtagata
      421 accacttatt caacaacttg aaaataaggt attttacctg ggttgttaca aaaggattgc
      481 attgcgtaaa cgctttttat ttacaaaaaa atggggaagt gttacagcga ttgcgaaaag
      541 cctgaagtga ttgaattaag ataatataga aaatcagata cagcaaaaat ggcagattga
      601 tcaggtgaag ataaattgga gcgggcgaag ggagccgagg acaactagct aacttcttgt
      661 ttcttaaaga ctgatgaatc cagtggttac agtgatggtt acacatggag aggctaaagg
      721 tcaagacttg aagtacgtaa cattcccaca aaaggaatag aacacagtca cggactaccc
      781 ggccctttgg tctgactgag ggtcgggcta cggggtaaat cgtcaggctt attgtattag
      841 atggtctctc acatttttca gtcaaaaact catcgtactt taggccagac catcagcatg
      901 actatcagag ccagctttag caggaagacc acgagataga ctttgtgtag gtccattagg
      961 tcctcctctt tccgtaccca tgaactagat tactagtaca aaataaaggc cagacatcca
     1021 gttacggaag tctgaccaat aagtcgttat tgttagttag cttgtcttag taaagctcag
     1081 gcgtttgact acaggcagga cagcagcagc gctgccagtc cccatcttaa cagtcgttgc
     1141 agcctcagcg accacgaaga ccttaccgag aatctcaagg tcgtgagact gggtgttgcc
     1201 atctcgggat aagaccacag taatattctc gccatcgtga cggaagcaac cgaagctcag
     1261 agccgtaaag cgcttagcca tcatgaagac gccacggtct gcaccgttgt a
//
LOCUS       DAJVQH010000284         1294 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJVQH010000284 DAJVQH010000000
VERSION     DAJVQH010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.290"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(71..538)
                     /locus_tag="OQM87_004258"
     CDS             complement(71..538)
                     /locus_tag="OQM87_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCS1510128.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(604..843)
                     /locus_tag="OQM87_004259"
     CDS             complement(604..843)
                     /locus_tag="OQM87_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCS1510129.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(978..>1294)
                     /locus_tag="OQM87_004260"
     CDS             complement(978..>1294)
                     /locus_tag="OQM87_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1510130.1"
                     /translation="KGVRYLGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPE
                     NDNSQEFSTEELLPIKHTDKNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQ
                     DK"
BASE COUNT          424 a          284 c          225 g          361 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaagcat ccgatgattc atttatgctg
       61 tcctaaaaaa ttaacaaaac aatgggaaaa caggatatgt aagcagaaaa gccaatgaaa
      121 ttggtacagc ataaggcaca ccgttttcac gaattgattt tcgaaaaaaa agaagacctg
      181 ccagaacaac cagagcgccg caaaaggtca ttgcgacgag aaaattagcc agttcatgtt
      241 catcaatagc taacgaaaga acagttaaca gcttgacatc accgccgcca attatccctg
      301 cggaaaaaag aaagaagccc acgagcaaaa cgctcccggc tgcaaccaga ttgggttttt
      361 gatgctgtaa aatgagtgcc aaaaaaataa gcacggttaa caccagtaaa taacgatggc
      421 taataattct gagtaatata tctccccatg aaataaaaat aagaaagagc gcaacaaata
      481 aaagatcgac tcttagtaac catattgctg ccattgaaag actccatgta ccatacaagg
      541 ttgctgccag acatattcct ttacgtctga cagcctctag agagtttttt tattttttat
      601 aagttacgga ttactcgttc cggaaatcgt tacctgttga attgctgcag cgatagcgtc
      661 gaatgcatct ttaattgctc ccaggaagcc tgagttctga tcgccaaaga tgaaagcaag
      721 cagagttgcc atagcaacac caatcaatgc atattcgatt gcagtaacac cacgttcatc
      781 cttcgcgaaa cgcgcaaagc actgtttagt tttaccatag tacttagtaa taaaagtatt
      841 catgttttcc tcgtatatta ttatttaaat tcaaaccacc agcttcatct gcgtgtcacc
      901 ggtatcccat atttttggga tgtggctata attatccatt cccgctattt agtcttcaaa
      961 aaaaattaca tttaatatca tttatcttga ttaagaaata gtaagactaa aaaaacaagc
     1021 gtcgtcagcc agcacactgc taacacggta agaacgctaa taaaaacact ccactgcttc
     1081 gttctatacc attcaatatt tttgtcagta tgttttattg ggagcagctc ttcagtagag
     1141 aattcctggc tgttatcatt ctcaggaatg tttgcatcag tgtttacttc cctggcctcg
     1201 ctatcgttgg tatagctact actgtcttca tcactgtcac gctcattctc acagacatgt
     1261 ttctcgacct ttcccagata tctgacgcct tttc
//
LOCUS       DAJVQH010000285         1263 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJVQH010000285 DAJVQH010000000
VERSION     DAJVQH010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.291"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            18..622
                     /locus_tag="OQM87_004261"
                     /pseudo
     CDS             18..622
                     /locus_tag="OQM87_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            609..1157
                     /locus_tag="OQM87_004262"
                     /pseudo
     CDS             609..1157
                     /locus_tag="OQM87_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          309 a          298 c          347 g          309 t
ORIGIN      
        1 actccaggag gcataacgtg cagaatgtgg ctgccactgt gcttgcgcag tatgctgcca
       61 gcccccgact caatgccctc attaacagct ttaacgcagc gctttccccg acagttttat
      121 cagtgatttt tatggtctta tctggaacat cgataccgca gaaaagtatg gtcttgatgt
      181 ctggggaaag attgtgggtg tcagtcgccg gctgacggta aaggacgatt ttaattacct
      241 gggcttcagc gagtccagga tggacacccc ggtaatggat gatccctgtc cgtttaatca
      301 ggcaccgttt tacaacggca aatcggatac ccggactgtt gacctgtctg atgctgtata
      361 ccggcggctg atactgatga aagccatgtc gaacattact gactgttccg ttccggatat
      421 taacctgatg ctgagattta tgttcggaaa aaaacgccgg gcttatgttc tgaataatgg
      481 cgggctgagg atgagttacg tctttgagtc cgcgctctcg ttggcagaac tggcgattat
      541 ccagtcgtcg ggtgcactgc catccccgcc gggtgtttat gtttcagtag ttttaaagga
      601 gtcccgtaat gaaggccagt gataaacccc gccagctggc ggtccccttt gcgagtaccg
      661 gagataaaaa ccgtatcccg gacaaggcga cacagcagac cagagagagc ggtaatgctg
      721 cgtatgattc aggttttcct ccggtgacca tgacagcggt ctcagcggga ggtataccgc
      781 cacacggcaa ggattttaac ggtctgatgt acgatattac cgcagcaata cggttcgccc
      841 aggctggcgg tttgtacacg tataatgccg gttttgcggg ggccattggt ggatatgcaa
      901 aaggagccat tctcgccgga gtcgcaacaa cagcggtctg gctgaatacc acggacgata
      961 acctgaccga tcctgaaggc tccgacagtg cgggctgggt aaatcttctt gaggatccga
     1021 aaaggatatt cctgcggcag aagaacaatc tgtcagacct tcagaataaa gggacggcac
     1081 gggataatct tcaggtttac agtaaagagc agtcagatca acgctatgtt catcgggaag
     1141 gcgataaaat aaccggagag ctgaaaatcc gtggtgttaa tgcgctgagg attttcaacg
     1201 aagcttttgg cctgattttt cgtcgttcgg aagagtgcct gcaccttatt cccaccagtg
     1261 aag
//
LOCUS       DAJVQH010000286         1205 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJVQH010000286 DAJVQH010000000
VERSION     DAJVQH010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.292"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            117..>1205
                     /gene="rstB"
                     /locus_tag="OQM87_004263"
     CDS             117..>1205
                     /gene="rstB"
                     /locus_tag="OQM87_004263"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCS1510131.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAP"
BASE COUNT          289 a          292 c          298 g          326 t
ORIGIN      
        1 gatacatccg tctgttccgg acattatctt ccaaacaagg tcttatccag ttggcggttt
       61 acattgctcc acagtcgtga attaatacga atagtattct gttgagggcg atgccaatga
      121 aaaaattgtt tatccagttt tacctgttat tgtttgtctg cttccttgtg atgtctctgc
      181 tggttgggct ggtgtacaaa tttaccgccg aacgcgcggg caaacagtcg ctggatgatt
      241 tgatgaacag ttcgctgtat ctgatgcgca gcgaattgcg agagatcccc ccacacgact
      301 ggggtaagac gctgaaagag atggatttaa atctctcttt cgatctgcgt gtcgagccac
      361 tgagtaaata ccatcttgat gatatttcca tgcaccgact gcgtggcggc gaaattgtcg
      421 ccctggacga tcagtacacg tttttgcagc gtatcccgcg cagccactac gtgctggcag
      481 ttggtcctgt tccttatctt tattacctcc atcaaatgcg attgctggat atcgccctga
      541 tcgcttttat tgctatttcc ctcgcctttc cggtgtttat ctggatgcgt ccgcactggc
      601 aggatatgtt aaaactggaa gcagcggcgc aacgatttgg cgatgggcat ctcaatgaac
      661 gtatccactt tgatgagggt tcgagctttg aacgacttgg tatcgcattt aaccagatgg
      721 cggacaatat caacgcctta attgccagca aaaaacagct tattgacggt atcgctcacg
      781 aactgcgaac accgttagtg cgcctgcgtt atcgactgga gatgagcgat aacctgagcg
      841 ccgccgaatc ccaggcgttg aatcgtgata tcagtcaact tgaagcttta attgaagagc
      901 tgctgactta tgcccgactc gatcgcccac aaaacgagct tcatcttagc gaaccagacc
      961 tgccgttgtg gctgtcaacg catctggcag atattcaggc agtaacgccc gataaaacgg
     1021 tacggataaa aacgctcatg caaggccatt atgcggcgtt ggatatgcgc ttaatggagc
     1081 gcgtgctgga taatttgctc aataacgccc tgcgctactg ccattcaacg gttgaaacca
     1141 gcctgctact gtcggggaat agagcgacat taattgttga ggatgatggc ccagggattg
     1201 cccca
//
LOCUS       DAJVQH010000287         1200 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJVQH010000287 DAJVQH010000000
VERSION     DAJVQH010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.293"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>1200
                     /locus_tag="OQM87_004264"
     rRNA            <1..>1200
                     /locus_tag="OQM87_004264"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          321 a          263 c          371 g          245 t
ORIGIN      
        1 accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga aggcccagac
       61 agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag ctcactggtc
      121 gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct gcggcagcga
      181 cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt actgtgaggt
      241 atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg ggtgaaaagc
      301 ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg tgagtcgacc
      361 cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc tgtacttggt
      421 gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg tcccggttta
      481 agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg tgatgacgag
      541 gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta agcatcaggt
      601 aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc aaggcgcttg
      661 agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag aaggcacgct
      721 gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata ccagctggct
      781 gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta tacggtgtga
      841 cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc tcttgatcga
      901 agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat tccttgtcgg
      961 gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac ccgagactca
     1021 gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa agaccccgtg
     1081 aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata ggtgggaggc
     1141 tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc tttaatgttt
//
LOCUS       DAJVQH010000288         1194 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJVQH010000288 DAJVQH010000000
VERSION     DAJVQH010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.294"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..805)
                     /locus_tag="OQM87_004265"
     CDS             complement(<1..805)
                     /locus_tag="OQM87_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1510132.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQR"
     gene            897..983
                     /locus_tag="OQM87_004266"
                     /pseudo
     CDS             897..983
                     /locus_tag="OQM87_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          336 a          208 c          318 g          332 t
ORIGIN      
        1 atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata ttaacatctg
       61 cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg ttagggatat
      121 atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt tccccaacgt
      181 ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta tcaaaagcac
      241 ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg ttgccaacag
      301 aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg agaagggctg
      361 agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg ttaagtgaaa
      421 catcaagagc gactaggtga ggcggaagag ccgggagcct gctcagtctg ttatcgctgg
      481 ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca ttgctagaga
      541 tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg agtaaactta
      601 aattgagggc tggctcttga aaagccagac atattttcag taatcgaact gcctgtgttc
      661 ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga taatccgtga
      721 tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag gagcggaaaa
      781 aagaaggatt gtggacaggt ttcatttatt attgatcctc attgtttata tattaatata
      841 ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt taagaacagg
      901 aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg ctcaatgtgc
      961 tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca gtagtctgcc
     1021 caccacagca tcatttctct gcgcttatcg agatactgag catggttgta aattcagcta
     1081 acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg cgctaatgct
     1141 ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga tatg
//
LOCUS       DAJVQH010000289         1192 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJVQH010000289 DAJVQH010000000
VERSION     DAJVQH010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.295"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..569
                     /locus_tag="OQM87_004267"
     CDS             <1..569
                     /locus_tag="OQM87_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCS1510133.1"
                     /translation="AHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQ
                     IFSWWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQM
                     AQESGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEH
                     LPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            566..>1192
                     /locus_tag="OQM87_004268"
     CDS             566..>1192
                     /locus_tag="OQM87_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCS1510134.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALAGGLPLYVSLYP
                     TEGGMQDIIDCIRAELGSGTTKNAVFQHIQSLPRGQQKEALLASAALPL"
BASE COUNT          268 a          295 c          354 g          275 t
ORIGIN      
        1 tggcgcatta ttccgcagat acgcgggagc agttactcac cctggccgat caggttcacc
       61 agaaactgaa tcatcttgaa gaaaaactcc accgtgttga cctggttcag cgggcgcagt
      121 tacatcttga gcagatattc tcctggtgga gcgccgggcg atatgcgtca ttttcccctg
      181 ccggacgttg ttatgtggca cttgaagagc ttcgctgggg cgcgtttggt gatgtgatac
      241 gtcagggcga aacaggccag gttaaccagc tactggatat tctcagacat aaagcattaa
      301 cgcagatggc acaggagagt ggcggtagtg caaccgtgcg tctgaacact ctggactggc
      361 ttggtggtca gggccgggag caggcagata acgagtggca tgacgcgatt aactggctgg
      421 gggactggtg cagtgaagag cagcatccgg tgatctggtc aaccacgcag gctgcagaac
      481 acttaccggt tcgcatgccc cgtctctgct ctgcagaacg cctctctgaa agtatggtcg
      541 atgaaatatt tcagaagggg gctgcatgag tatagaaata aaaacagggc tggtgctgtc
      601 cggcggtggg gcggtgggcg cttatcaggc gggagtggtt aaggcactgg cagagtgtgg
      661 tacacagatc agcatggttt caggggccag cattggcgca ttcaatggtg ccattatcgc
      721 ggcctctccc gatctgtcag aagctgccgt acgcctggag gcgctctggg atcatcttgg
      781 gaataatcag gtgctgtcgg taaaccgatc ggtttacttt tcattgctga aaaaattgtt
      841 ccaggcaatg aacctctgcc agatccccgg acgtgcagga gcactgctta cgacacttct
      901 tcgccatata tcgataatca acggcttcga caatccgatg actcagccgt tgttgtcaga
      961 tgggcccctg acagcgttga tggatcatta tcttgataca gatgctctgg caggcgggct
     1021 accgctgtat gtgtcgctgt accccacaga agggggcatg caggatatta ttgactgcat
     1081 tcgtgctgaa ctgggttccg gaaccacgaa aaacgccgtt tttcagcata tccagagtct
     1141 gccccgcgga cagcagaaag aggctctgct tgcgtcagcc gcactgcccc tg
//
LOCUS       DAJVQH010000290         1185 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJVQH010000290 DAJVQH010000000
VERSION     DAJVQH010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.296"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..180
                     /locus_tag="OQM87_004269"
                     /pseudo
     CDS             1..180
                     /locus_tag="OQM87_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            197..715
                     /locus_tag="OQM87_004270"
                     /pseudo
     CDS             197..715
                     /locus_tag="OQM87_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          359 a          272 c          255 g          299 t
ORIGIN      
        1 aacggaatta tcgtcggtcg tgacagactg gcacgtcttc gtaaggagct gaggctgcac
       61 tgtaagcaga aacgcaagtt cagggcgacc acaaactctg accacaatct gccggttacg
      121 ccgaatctgc tgaatcagaa ctttacgccc acagcaccta atcaggtctg ggtcgcagat
      181 tcagttgtcc aggccttccg aaatcaaccg actgaaggcg ctggtcgcga aactgcagcg
      241 tatgcagttc ggtaaaagct cagaaaaact tcgcgcaaaa accgaacggc ggatacagga
      301 agcacaggag cgaatcagcg cacttcagga ggaaatggcg gaaacactgg gtgagcaata
      361 tgacccggta ctgccatctt ccctgcgcca gtcttcagcc cgtaaaccgt taccagcctc
      421 acttccccgt gccccccggg tcatccggcc ggaagaggaa tgctgtccgg cctgtggtgg
      481 tgaactcagt ccactgggat gtgatgtgtc agagcagctg gagcttatca gcagcgcctt
      541 taaggttatc gaaaaacaac gccctaaact ggcctgtcgc cggtgcgacc atatcgtgca
      601 ggcaccggtg ccttcaaaac ccattgcacg cagttatgcc ggagcggggc ttctggccca
      661 tgttgttacc ggaaagtatg cagaccatct gccattatac cgccaaagtg atctactatt
      721 ccacacagct atttagctct ctttttgttg agctcccatg attctttcat ttattgttac
      781 taattatttt atgttatcat gatgttagaa cacgctctgc tctgtgtctc tattttatct
      841 atcatattct gaggattaac tacataacaa ggcgcaagat atatatccaa aaacaaataa
      901 taccgttgca ataattcctt tcagtaaagg aggtgtgatt aatttcaggg gatttcagag
      961 taaaaattgt acaacatatt aaaatatgtt catttcgtta taatacacac aagaaaatat
     1021 taagttagat aatatattct aaataaccat agataaaaac gcacataatg gggaatattt
     1081 tatgaaaata cctgaagcag taaatcatat taatgttcag aataatattg atcttgttga
     1141 tggaaaaacg aatccgaata aagcaacaaa agcattacaa aaaat
//
LOCUS       DAJVQH010000291         1152 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJVQH010000291 DAJVQH010000000
VERSION     DAJVQH010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.297"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..279
                     /locus_tag="OQM87_004271"
                     /pseudo
     CDS             1..279
                     /locus_tag="OQM87_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            207..680
                     /locus_tag="OQM87_004272"
                     /pseudo
     CDS             207..680
                     /locus_tag="OQM87_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT          287 a          283 c          345 g          237 t
ORIGIN      
        1 agttccgtca gcactgcgac agcgttgtcc gcaaggcttt tacccagtcc agcgttacgg
       61 tgccccacgc ctgacggatg aactgtgtgc tctggttacc actttaacgt aaaaaccgtg
      121 gcagccagtc tgcaccgtca ggaactgagc gcaaaagcct cccagaagtt cagcccgatc
      181 agttaccgtg cacacggcct gcctgtatca gaaaatctgc tgacgcagga tttttacgcc
      241 agtggcccga accagaagtg ggcgggagac atcacgtact attacagctc accgactgcg
      301 gggaaacacg gggcgccggg ttattgaagg tgacctgtcc agctacttcg acacagtaca
      361 tcaccggctg ctgatgaaag ccgtctgccg caggatcagt gatgcacgtt ttatgagact
      421 gctgtggaaa acgccgtgtt gatgttggtc tcttccgggc ggtcagtgaa agtatgccac
      481 agggcggtgt gatctcgccg ttgctgtcga acatcatact gaatgagttc gaccagtatc
      541 tgaataaacg ctacctgagt ggaaaagcca gaaaggatcg ctggtactgg aatcacagta
      601 tccagcgagg ccgaagtact gctgtcaaag aaaactggca atggaaacct gccgttgctt
      661 actgttgcta tgccgatgac tgtgtgccac gaaggcgcgt cctggggacg tgagtccctg
      721 tgcagccatg cagcacaaga gatgagggaa ggcccctcgg tgtcggcgtg caggcgcagg
      781 caccaaacca tccactgctg ctgcatttaa gagatgtggt ggtgagcgag atgggaaaag
      841 gtcggacgtg agccgatcag gtatggttcg aagagacgaa cgaaagtgaa ccgctgatga
      901 tgtgtcgaaa gcatatagat gatgtcaaaa caatggtgga gtcgttcctg tgggatatgt
      961 ttactgcctg cgcggcatcc ggcataaaag cggcgtgaag tcgattcagg ctcttgtggg
     1021 gaacgtggac ctgtcacccc gatgtgaagg gagaagcgca agtggaagcc ccatgagcgc
     1081 cagagtacca atgcggggta cagggacgga gcaccaagcg attgcgcagt aaacatgctt
     1141 gacaacttaa tt
//
LOCUS       DAJVQH010000292         1129 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJVQH010000292 DAJVQH010000000
VERSION     DAJVQH010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.298"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..474
                     /locus_tag="OQM87_004273"
     CDS             <1..474
                     /locus_tag="OQM87_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCS1510135.1"
                     /translation="FGFFVRLDDLFIDGLVHVSSLDNDYYRFDQVGQRLMGESSGQTY
                     RLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTAREKAKKGDAGKKGGKRRQVG
                     KKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIAAATKAKRAAKKKVAE"
     gene            654..>1129
                     /gene="rlmB"
                     /locus_tag="OQM87_004274"
     CDS             654..>1129
                     /gene="rlmB"
                     /locus_tag="OQM87_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCS1510136.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLAR"
BASE COUNT          306 a          284 c          320 g          219 t
ORIGIN      
        1 tttggcttct tcgtccgtct ggacgacttg ttcattgatg gtctggtcca tgtctcttcg
       61 ctggacaatg actactatcg ctttgaccag gtagggcaac gcctgatggg ggaatccagc
      121 ggccagactt atcgcctggg cgatcgcgtg gaagttcgcg tcgaagcggt taatatggac
      181 gagcgcaaaa tcgactttag tctgatctcc agtgaacgcg caccgcgcaa cgtcggtaaa
      241 acggcgcgcg agaaagcgaa aaaaggcgat gcaggcaaaa aaggcggcaa gcgtcgtcag
      301 gtcggtaaaa aggtaaactt tgagccagac agcgccttcc gcggtgagaa aaaaacgaag
      361 ccgaaagcgg cgaagaaaga cgcgagaaaa gcgaaaaagc catcggcgaa aacgcagaaa
      421 atagccgcag cgaccaaagc gaagcgtgcg gcgaagaaaa aagtggcaga gtgatcaata
      481 ccctctttaa aagaagaggg ttagattgct gacaaaatgc gctttgttca tgccggatgc
      541 ggcgtgaacg ccttatccgg cctacataat cacgcaaatt caatatattg cagagatcat
      601 gtaggccgga taaacgtagt gcatcaggca aaacgtaaac aacgagtaca ttaatgagcg
      661 aaatgattta cggcatccac gcagtgcagg ccctgctgga gcgcgcccct gaacgttttc
      721 aggaagtctt tattttaaaa ggccgtgaag ataaacgtct gttaccgctg attcacgccc
      781 ttgagtctca gggcgtggtt atccagttgg caaaccgcca atatctcgac gagaaaagcg
      841 acggtgccgt gcatcagggc attatcgccc gcgtgaagcc aggacgtcag tatcaggaaa
      901 acgatctgcc ggatctgatc gcttcgctcg atcaaccgtt cctgctgatc ctcgacggcg
      961 taaccgatcc gcacaacctc ggcgcgtgcc tgcgtagtgc ggacgccgca ggcgttcatg
     1021 cggtgattgt gccgaaagat cgctccgcac agctcaacgc tacggcgaaa aaagtagcct
     1081 gcggcgcggc agaaagcgtt ccgctgattc gggtgactaa ccttgcgcg
//
LOCUS       DAJVQH010000293         1112 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJVQH010000293 DAJVQH010000000
VERSION     DAJVQH010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.299"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..1019
                     /locus_tag="OQM87_004275"
                     /pseudo
     CDS             1..1019
                     /locus_tag="OQM87_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          315 a          272 c          256 g          269 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgagaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga gattccacaa acccagcaag
      841 gggacattgg cgcgaaagat attccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag cc
//
LOCUS       DAJVQH010000294         1100 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJVQH010000294 DAJVQH010000000
VERSION     DAJVQH010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.300"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            78..>1100
                     /gene="pykF"
                     /locus_tag="OQM87_004276"
     CDS             78..>1100
                     /gene="pykF"
                     /locus_tag="OQM87_004276"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCS1510137.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNS"
BASE COUNT          309 a          273 c          268 g          250 t
ORIGIN      
        1 taccggattc gctttccggc agtgcgccca gaaagcaagt ttctcccatc cttctcaact
       61 taaagactaa gactgtcatg aaaaagacca aaattgtttg caccatcgga ccgaaaaccg
      121 aatctgaaga gatgttagct aaaatgctgg acgctggcat gaacgttatg cgtctgaact
      181 tctctcatgg tgactatgca gaacacggtc agcgcattca gaatctgcgc aacgtgatga
      241 gcaaaactgg taaaaccgcc gctatcctgc ttgataccaa aggtccggaa atccgcacca
      301 tgaaactgga aggcggtaac gatgtttctc tgaaagctgg tcagaccttt actttcacca
      361 ctgataaatc tgttatcggc aacagcgaaa tggttgcggt aacgtatgaa ggtttcacta
      421 ctgacctgtc tgttggcaac accgtactgg ttgacgatgg tctgatcggt atggaagtta
      481 ccgccattga aggtaacaaa gttatctgta aagtgctgaa caacggtgac ctgggcgaaa
      541 acaaaggtgt gaacctgcct ggcgtttcca ttgctctgcc agcactggct gaaaaagaca
      601 aacaggacct gatctttggt tgcgaaaaag gcgtagactt tgttgctgct tcctttattc
      661 gtaagcgttc tgacgttatc gaaatccgtg agcacctgaa agcgcacggc ggcgaaaaca
      721 tccacatcat ctccaaaatc gaaaaccagg aaggcctcaa caacttcgac gaaatcctcg
      781 aagcctctga cggcatcatg gttgcgcgtg gcgacctggg tgtagaaatc ccggtagaag
      841 aagttatctt cgcccagaag atgatgatcg aaaaatgtat ccgtgcacgt aaagtcgtta
      901 tcactgcgac ccagatgctg gattccatga tcaaaaaccc acgcccgact cgcgcagaag
      961 ccggtgacgt tgcaaacgcc atcctcgacg gtactgacgc agtgatgctg tctggtgaat
     1021 ccgcaaaagg taaatacccg ctggaagcgg tttctatcat ggcgaccatc tgcgaacgta
     1081 ccgaccgcgt gatgaacagc
//
LOCUS       DAJVQH010000295         1091 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJVQH010000295 DAJVQH010000000
VERSION     DAJVQH010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.301"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..942
                     /locus_tag="OQM87_004277"
     CDS             <1..942
                     /locus_tag="OQM87_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HCS1510138.1"
                     /translation="TGLGTITAANTVTVRSRVNGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
     gene            942..>1091
                     /locus_tag="OQM87_004278"
     CDS             942..>1091
                     /locus_tag="OQM87_004278"
                     /inference="COORDINATES: protein motif:HMM:NF013069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCS1510139.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVD"
BASE COUNT          287 a          300 c          294 g          210 t
ORIGIN      
        1 accgggcttg gcaccattac cgccgctaat actgttacgg tgcgcagccg cgtgaacggt
       61 caactgatgg cgttacattt ccaggaaggc cagcaggtca aagcaggcga tttactggca
      121 gaaattgacc ccagccagtt caaagttgca ttagcacaaa cccagggcca actggcaaaa
      181 gataaagcca cgcttgctaa cgcccgccgt gatctggcgc gttatcagca actggcaaaa
      241 accaatctcg tctcccgtca ggagctggat gcccaacagg cgctggtcag tgaaaccgaa
      301 ggcaccatta aggctgatga agcaagcgtc gccagcgcgc agttgcaact cgactggagc
      361 cgcatcaccg caccagtcga tggtcgcgtt ggtctcaagc aagttgatgt tggtaaccaa
      421 atctccagtg gtgataccac cggaattgtg gtgatcaccc agacgcatcc tatcgattta
      481 gtctttaccc tgccggaaag cgatatcgct actgtagtgc aggcgcaaaa agccggaaaa
      541 ccgctgatgg tagaagcctg ggatcgcacc aattcgaaga aattaagtga aggcacgctg
      601 ttaagcctcg ataaccaaat cgatgccaca accggtacga ttaaagtgaa agcacgcttt
      661 aataatcagg atgatgcgct gtttcccaat cagtttgtta acgcgcgcat gttagtcgac
      721 accgaacaaa acgccgtagt gatcccaaca gccgccctgc aaatgggcaa tgaaggccat
      781 tttgtctggg tgctgaatag cgaaaacaag gtcagcaaac atctggtgac gccgggcatt
      841 caggacagtc agaaagtgga agtggtggaa gcccagagcg ccaccactcc ggaagagaaa
      901 gccaccagcc gcgaatacgc gaaaaaagga gcacgctcct gatgcaggtg ttacccccga
      961 gcagcacagg cggcccgtcg cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga
     1021 tggtggcgat cttactcgcc gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc
     1081 cggaagtgga c
//
LOCUS       DAJVQH010000296         1089 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJVQH010000296 DAJVQH010000000
VERSION     DAJVQH010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.302"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..122)
                     /locus_tag="OQM87_004279"
     CDS             complement(<1..122)
                     /locus_tag="OQM87_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1510140.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHL"
     gene            complement(194..1078)
                     /locus_tag="OQM87_004280"
     CDS             complement(194..1078)
                     /locus_tag="OQM87_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCS1510141.1"
                     /translation="MVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDD
                     PFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETT
                     DLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLL
                     RLCLRQALQLADVVKISEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVC
                     YRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGAL
                     AVTAKGAMTALPCRQELE"
BASE COUNT          291 a          313 c          229 g          256 t
ORIGIN      
        1 agatgtcctt tcagccaaat agcgtataac gaccaccaca gcgaccagga aataaaaaag
       61 agaaatgagt aactggatgc aaaacgatag tacgcatttc tgaatggaat attcagtgcc
      121 ataattacct gcctgtcgtt aaaaaattca cgtcctattt agagataaga gcgacttcgc
      181 cgtttacttc tcactattcc agttcttgtc gacatggcag cgctgtcatt gcccctttcg
      241 ccgttactgc aagcgctccg caacgttgag cgagatcgat aattcgtcgc atttctctct
      301 catctgtaga taatcccgta gaggacagac ctgtgagtaa cccggcaacg aacgcatctc
      361 ccgcccccgt gctatcgaca caattcacag acattccagc aaaatggtga acttgtcctc
      421 gataacagac caccacccct tctgcacctt tagtcaccaa cagcatggcg atctcatact
      481 cttttgccag ggcgcatata tcctgatcgt tctgtgtttt tccactgata agtcgccatt
      541 cttcttccga gatcttgacg acatccgcca gttgtagcgc ctgccgcaaa cacaagcgga
      601 gcaaatgctc gtcttgccat agatcttcac gaatattagg atcgaagctg acaaaacctc
      661 cggcatgccg gatcgccgtc atcgcagtaa atgcgctggt acgcgaaggc tcggcagaca
      721 acgcaattga acagagatgt aaccattcgc catgtcgcca gcagggcaag tctgtcgtct
      781 ctaaaaaaag atcggcactg gggcggacca taaacgtaaa tgaacgttcc ccttgatcgt
      841 tcagatcgac aagcaccgtg gatgtccggt gccattcatc ttgcttcaga tacgtgatat
      901 cgactccctc agttagcagc gttctttgca ttaacgcccc aaaaggatca tcaccgaccc
      961 gacctataaa cccacttatt ccgcctaatc tggcgattcc caccgcaacg ttagctggcg
     1021 cgccgccagg acaaggcagt aggcgcccgt ctgattctgg caagagatct acgaccacat
     1081 cccctaaaa
//
LOCUS       DAJVQH010000297         1046 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJVQH010000297 DAJVQH010000000
VERSION     DAJVQH010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.303"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..98)
                     /locus_tag="OQM87_004281"
     CDS             complement(<1..98)
                     /locus_tag="OQM87_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCS1510142.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGR"
     gene            complement(95..385)
                     /locus_tag="OQM87_004282"
     CDS             complement(95..385)
                     /locus_tag="OQM87_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCS1510143.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(385..984)
                     /locus_tag="OQM87_004283"
     CDS             complement(385..984)
                     /locus_tag="OQM87_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCS1510144.1"
                     /translation="MAHIQLVKQTSSGLLLPATSESCEFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYRTPVGGAITPRERELVSGFVEYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          234 a          281 c          250 g          281 t
ORIGIN      
        1 cgcccccaca tgcgcagttt tccctgaatc cagacacttt ccagcgtggt gagacgaagg
       61 tgttccccgc ttttgcctgt atttgttggg taaatcataa ataaccttcc tttctccaga
      121 tttcttgtgt gcgaaaaaca ccttctgcat gcatcaggcg taattcttct ttggtgtaat
      181 cgctggtttt tacccgcccg tcgattaaat cgtggcatga gctgcaggca atcgctgcct
      241 gcatatcgtg tggttttgtc gctgttccgc acgtccccgc cagcctgtaa tgcgccagca
      301 cagaagtttc gggattgtga ttgcagtagc caggaattct gacggtgcac atctgccccc
      361 gcgccgcttt acgtaaatcc accattacgc aaactccagt aactgtgcgg ccacattttc
      421 gacttcctcc ggagaggaaa atttacggaa caggatccag ttccacagca cattcagtac
      481 agatttataa acctgctgaa actcggtttc gtccatgttc gcaaatgcga tagattttgc
      541 cctgcgccca cggctaccgt ccggataaat atgctcggtg taaaatccgg cctgaatggt
      601 tacccactcg cggaaagcct caaacgactt tagcaatgcc gtatcccggg ttctgcgtgt
      661 cgcaacggta ttcagatatt gctctgcggc ttcgctcaga gctggcgtat gttcccgacc
      721 tactgattcg cacaggtact caacgaagcc tgataccagt tctcgttctc gaggcgtgat
      781 cgccccaccg accggagtcc ggtaatcgaa tcccagttgc aggagtttga aaaaacgctt
      841 gtggaatgcg tagttacgaa cgcgcttaaa atcagcgtgt atccactcgc ctgttttgat
      901 ttgatgcaga aattcgcaac tctccgacgt cgccgggaga agtaatccgg aagaggtttg
      961 tttgaccagt tgtatatgcg ccatcgtagt tctccgctgg cgcagtagaa tgggtgttca
     1021 gcccgttatg tagtatacca gaatta
//
LOCUS       DAJVQH010000298         1042 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJVQH010000298 DAJVQH010000000
VERSION     DAJVQH010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.304"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..336
                     /locus_tag="OQM87_004284"
     CDS             <1..336
                     /locus_tag="OQM87_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1510145.1"
                     /translation="RRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQ
                     YRKGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQIL
                     IREIKGSSH"
     gene            336..683
                     /gene="tnpB"
                     /locus_tag="OQM87_004285"
     CDS             336..683
                     /gene="tnpB"
                     /locus_tag="OQM87_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1510146.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            703..>1042
                     /locus_tag="OQM87_004286"
     CDS             703..>1042
                     /locus_tag="OQM87_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1510147.1"
                     /translation="MDTSLSHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPILPS
                     PLRQSSARKPL"
BASE COUNT          255 a          291 c          280 g          216 t
ORIGIN      
        1 cggcggccta attttcctta tgaatttaaa atcgctttag tggagcagtc actgcagccc
       61 ggagcctgtg tggcgcagat cgcccgtgaa aacggaatca acgataacct gctcttcaac
      121 tggcgccatc aataccggaa aggtggcctg ctgccttccg gaaaaaatat gcctgcactg
      181 cttcccgtga cgttaacgcc ggagcagata aatacaccgt ccgacagtct gtgctgtgag
      241 ctggttctgc cggccggaac tctcaggctt aaaggtaaac tgacgccggc gttattacag
      301 atacttatcc gcgaaataaa agggagcagc cactgatgat atctttccct gcaggttcgc
      361 gtatctggct ggttgcaggt atcaccgata tgcgaaatgg ctttaacggc ctggcctcaa
      421 aagttcagaa cgtcctgaag gatgacccgt tctccgggca tctgttcatc ttccgcggac
      481 gccggggtga ccagataaaa gtgttgtggg ctgacagtga cggactgtgc ctctttacca
      541 gacgcctgga gcggggccgc ttcgtctggc cggtcacccg tgacggcaag gtgcacctta
      601 ctccggccca gttatccatg cttcttgagg gcatcgactg gaaacacccg aaacgaacgg
      661 aacgcgctgg catccgcata taacccgttg taaagtgagg atatggacac ctcactttct
      721 catgagaatg cccgcctgcg ggcactgttg cagacgcaac aggacaccat ccgccagatg
      781 gcgaaataca accgcctgct ctcacagcgg gtggcggctt atgcttccga aatcaaccgg
      841 ctgaaggcgc tggttgcgaa actgcagcgc atgcagttcg gtaaaagctc agaaaaactt
      901 cgcgcaaaaa ccgaacggca gatactggaa gcacaggagc gaatcagcgc acttcaggaa
      961 gaaatggcgg aaacgctggg tgagcaatat gacccgatac tgccatctcc cctgcgccag
     1021 tcttcagccc gtaaaccgtt ac
//
LOCUS       DAJVQH010000299         1040 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJVQH010000299 DAJVQH010000000
VERSION     DAJVQH010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.305"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>1040
                     /locus_tag="OQM87_004287"
     rRNA            <1..>1040
                     /locus_tag="OQM87_004287"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          258 a          242 c          329 g          211 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaaccttacc tggtcttgac atccacggaa gttttcagag atgagaatgt gccttcggga
      541 accgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt tgggttaagt
      601 cccgcaacga gcgcaaccct tatcctttgt tgccagcggt ccggccggga actcaaagga
      661 gactgccagt gataaactgg aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg
      721 accagggcta cacacgtgct acaatggcgc atacaaagag aagcgacctc gcgagagcaa
      781 gcggacctca taaagtgcgt cgtagtccgg attggagtct gcaactcgac tccatgaagt
      841 cggaatcgct agtaatcgtg gatcagaatg ccacggtgaa tacgttcccg ggccttgtac
      901 acaccgcccg tcacaccatg ggagtgggtt gcaaaagaag taggtagctt aaccttcggg
      961 agggcgctta ccactttgtg attcatgact ggggtgaagt cgtaacaagg taaccgtagg
     1021 ggaacctgcg gttggatcac
//
LOCUS       DAJVQH010000300         1028 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJVQH010000300 DAJVQH010000000
VERSION     DAJVQH010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.306"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OQM87_004288"
     CDS             complement(<1..692)
                     /gene="ospC2"
                     /locus_tag="OQM87_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCS1510148.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLT"
BASE COUNT          334 a          151 c          176 g          367 t
ORIGIN      
        1 tcggttaatg tcatatatcc atatggagag tcatgatcaa tgatatacgc attagcacca
       61 aaatccacag tatgatgtgt tttatttagt gggagtgttt cttgatgatt ggaaatttca
      121 accccaaaaa aaacaaaatc gtggttagct atttcttcaa tatcctcaga gaatgtgttt
      181 ttatatgttt gtatattctc agacttaatt ttatcaaagg acttaatatt aagagtatta
      241 tttgacaggt tagcgttact ttgatgtctg aagttaaaat cagaattaat aataatagag
      301 agcaagtgac gttcatccga agaaagagga tgagtttttt gtattatttc agaaagatag
      361 tgtctggaca ccttatcgag gtgctcaagt gaccgaaatg atgaccgtgc tccagatggc
      421 gcttcaatag aaagatttaa tgatttgaaa ctggtccctt gagagaacat tttactataa
      481 ctttgcgctg ctatagactt ccttaaaaag cttttcacag tatcagcaca gtgatctaaa
      541 tgtttttcac ttatgccaga agaggatgag tttgttacgc atgatatgtt tttttgtaat
      601 gcttttgtat ctttgttcgg atttattttt ccatcaacaa gatcaatatt gttctgaaca
      661 ttaatatgat ttactgcttc aggtattttc ataaaacatc ccccattatg tgcgttttta
      721 tctgcagtta tttagaataa tattatctaa cttaatattt tcttgtatgt attataacga
      781 acatatttta atatgttgta cgattttcac tctgaaatcc cctgagttta atcacacttc
      841 ctttactgaa aggaattatt gcaacagtat tatttgtttt tggatatata tcttgcgcct
      901 tgttatgtag ttaatgctca gaatatgata gataaaatag agacgcaaaa cagggcatgc
      961 tcataacatg atgataacat aaaaaattag taacaataaa tgaaagaatc atgggcgctc
     1021 aacaaaaa
//
LOCUS       DAJVQH010000301          998 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJVQH010000301 DAJVQH010000000
VERSION     DAJVQH010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.307"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..388)
                     /locus_tag="OQM87_004289"
     CDS             complement(<1..388)
                     /locus_tag="OQM87_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCS1510149.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQVDLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNV"
     gene            817..>998
                     /locus_tag="OQM87_004290"
     CDS             817..>998
                     /locus_tag="OQM87_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510150.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLG"
BASE COUNT          273 a          223 c          224 g          278 t
ORIGIN      
        1 aaacgttagg ttcacgctta taatcggccg gtgcaatcgt atagccgttt acaggcgcta
       61 tcagcgatga gcataaaaaa tggttatcac gggcatagat caattcatta atataaagat
      121 agccacgaat aatattcaac attcgctttt gatgggctgg agtgcaaact tgcccctgat
      181 agcgttcagc tgcatcgctc actaaatcta cctggcgaat gaccagctcg gatttatcca
      241 gtgccagttg agcaaaggtt cggagctgat tattcacttc tgattttgcc caaaggaagg
      301 caatccatag cgaaagaatg atgggaaaga gaactaaaaa gataatgccc ggcaacgcca
      361 gtaattggtg tcgtgcacga tgactcattg ctatcaattt cctgcctgtt tatacccggc
      421 cattgcaagg tgaaaccctg gctaccgacg tctttagata aatcttacgg tataaatagc
      481 acttttgcct gatttgaaat tgataccttt ttaatcgtca cctggatgaa aataattcat
      541 tgatattcaa aattaatttt ctacaggaat acagaggcgg cgggaagata tgcccttaat
      601 gccactgaaa aaccacataa tctgtacagc aataaaagtc acggcctaat atggctaaca
      661 aacaatcaat tgtgatatgg ttcacaaaat taatgaaaca aacagagtgt ttcatttttg
      721 tgttctgcaa gccccgcgat agccagagtg taccacgcct cccgtgaaca acgccgcgct
      781 gtccagggca tcggctcttt tcagaggaat tgtttgatgg ctaccctcac cactggcgtg
      841 gttcttcttc gctggcaact tcttagtgcc gtaatgatgt ttctggccag cacactcaac
      901 atccgttttc gtcggtctga ttatgtcggg cttgcggtga tcagcagcgg tctgggcgtt
      961 gtttccgcct gttggttcgc aatggggttg cttggcat
//
LOCUS       DAJVQH010000302          998 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJVQH010000302 DAJVQH010000000
VERSION     DAJVQH010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.308"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..757
                     /locus_tag="OQM87_004291"
     CDS             <1..757
                     /locus_tag="OQM87_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1510151.1"
                     /translation="DNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQQTK
                     NLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRTIHL
                     VQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGPHYK
                     VDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNV
                     YGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
     gene            778..>998
                     /locus_tag="OQM87_004292"
     CDS             778..>998
                     /locus_tag="OQM87_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCS1510152.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRD"
BASE COUNT          322 a          221 c          228 g          227 t
ORIGIN      
        1 agataatggt tatacatcag gaaatccatt taacggtctg acaccattaa aaaagtccaa
       61 gatagaacca gatcccctca cccgtgacga attcattcgt tttattgagg cttgccgtca
      121 tcaacaaaca aaaaacctgt ggattatcgc tgtatacacg ggtattcgtc acggggagct
      181 ggtatcactg gcctgggaag atatagacct taaagcgagg actataacca tccgccggaa
      241 ttatacaaaa ctaggcgaat tcactccacc aaaaactgat gctggtaccg gaagaacaat
      301 tcatctagtt caaccagcta ttgatgctct taaaagccaa gcggaaatga ccatgcttgg
      361 aaaacagcat tctgtagagg taaagcagag ggaatatggg agaagtactg tgcataaatg
      421 cacttttgtt tttagtcctc aggtaataaa acagcggcag ttttccggac cgcactataa
      481 ggttgactcc atcagggagt catggacaag tatcttaaaa cgcgcaggtc tgagacacag
      541 aaaatcgtat caatccaggc atacctatgc atgctggtca cttgccgcag gagctaatcc
      601 tagttttatc gcaagccaga tgggccacac aaacgcacaa atggtattca atgtttacgg
      661 agcatggatg aaagacaaca atcacgaaca gatagaactt cttaacagaa gactatctga
      721 aagtgtccca tgcatgcccc ataagaaagc ggggtaaaat aaaaacttgc aaaatcaatt
      781 ggtttacctt taatccctgt cacgttacgc gcgtggcaga ggcgttacgg attgctgaaa
      841 ccgcaacgga cagacggcgg tcatcggctg ttcaacgatg ccgatattga ccggatccgc
      901 gagatcaaac gctggatcga caacggcgtg caggtcagca aagttaaaat actgctcagt
      961 aatgaaaatg ttgatgtgca gaacgtctgg cgcgatca
//
LOCUS       DAJVQH010000303          983 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJVQH010000303 DAJVQH010000000
VERSION     DAJVQH010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.309"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OQM87_004293"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OQM87_004293"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCS1510153.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLHTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          219 c          221 g          284 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctt cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtatgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAJVQH010000304          965 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJVQH010000304 DAJVQH010000000
VERSION     DAJVQH010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.310"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..>965
                     /locus_tag="OQM87_004294"
     rRNA            1..>965
                     /locus_tag="OQM87_004294"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          271 a          210 c          295 g          189 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc acatactgtg
      361 agctcgatga gtagggcggg acacgtggta tcctgtctga atatgggggg accatcctcc
      421 aaggctaaat actcctgact gaccgatagt gaaccagtac cgtgagggaa aggcgaaaag
      481 aaccccggcg aggggagtga aaaagaacct gaaaccgtgt acgtacaagc agtgggagca
      541 cgcttaggcg tgtgactgcg taccttttgt ataatgggtc agcgacttat attctgtagc
      601 aaggttaacc gaatagggga gccgaaggga aaccgagtct taactgggcg ttaagttgca
      661 gggtatagac ccgaaacccg gtgatctagc catgggcagg ttgaaggttg ggtaacacta
      721 actggaggac cgaaccgact aatgttgaaa aattagcgga tgacttgtgg ctgggggtga
      781 aaggccaatc aaaccgggag atagctggtt ctccccgaaa gctatttagg tagcgcctcg
      841 tgaattcatc tccgggggta gagcactgtt tcggcaaggg ggtcatcccg acttaccaac
      901 ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga gacacacggc gggtgctaac
      961 gtccg
//
LOCUS       DAJVQH010000305          957 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJVQH010000305 DAJVQH010000000
VERSION     DAJVQH010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.311"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..608)
                     /locus_tag="OQM87_004295"
     CDS             complement(<1..608)
                     /locus_tag="OQM87_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HCS1510154.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVASYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDV"
BASE COUNT          273 a          269 c          197 g          218 t
ORIGIN      
        1 cagacatcca ctgagctgtt taactcattg acgcaatacg catattgttc gtttggatgg
       61 aataccatat gacgcgggcc ggccccttca acggtagtca cttccgcagg gtcctgcgcc
      121 accagatgac catcatcgct gaccgtaaac aggcaaatgc gatcttgctt taatgccgga
      181 acccacagcg tacggttgtc cggtgagata ttggcggaat ggcaaccgtc cagcccctcg
      241 actacatcga cgacgcccac tggcaggcca tcttccagac gcgttacgct cacgttaccc
      301 gcattgtaag aagctacaaa gacaaactgc ccctggtgat cggtggaaat atgcgtcgga
      361 ctacccggca gcacagactc tgcggcaaag gtcagtgcgc catcgtccgg ggcaatacga
      421 tacgccagga cgcgaaactc agggcgaaca ccaacataga gataacgttt gtccgggctg
      481 accaccatcg gctgcacctg ccccggcaca tcgacaacct gtgtcagcgt cagtgcgcct
      541 tcatgattca ggttccagac gtgaatttgc tggctctcag ggctggcgat ataaactgtt
      601 tgcttcatga acacacctca aaaatgataa atcccttatc tttcatacat ataacaacac
      661 ttactggata aaaaacagac aacaaaacac gataaaatac aatatcgcac atcaattaaa
      721 tcaataagtt agcactccat ttcgaatggc atattactaa aatattagtc accgacacga
      781 atttgacaat gccacagata ttttcgcgac tattcaggga gtatgactct ccccgctttt
      841 gtttacctgt atcaacgacc gtaaacacta tgccataaca acttctggcc aacttcccat
      901 tcgctcaaca atcaactcac ttccggcaca aataaacgca ttacgttgta gatcggt
//
LOCUS       DAJVQH010000306          941 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJVQH010000306 DAJVQH010000000
VERSION     DAJVQH010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.312"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            56..941
                     /locus_tag="OQM87_004296"
                     /pseudo
     CDS             56..941
                     /locus_tag="OQM87_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
BASE COUNT          246 a          217 c          240 g          238 t
ORIGIN      
        1 ttttattcat ataacaaaac atatagcttg ccatatttaa atttaaggaa ttgttatggg
       61 aaataatttg ttatcagcaa aagcgacact ccctgtttat gatcgtaata acctggcccc
      121 aagaattgtt catttaggct ttggtgcatt tcaccgtgcg catcagggcg tgtatgccga
      181 tattcttgct acagaacatt tcagtgactg gggatattat gaggtcaact taatcggcgg
      241 cgaacagcaa attgccgatt tacaacagca agataatctt tataccgttg cggaaatgtc
      301 ggccgatgcg tggacggctc gcgtcgttgg cgtcgttaaa aaagccttgc acgtacagat
      361 ggatggctta gaaaccgtgt tggctgcgat gtgtgaaccg caaatcgcga ttgtctctct
      421 gacaatcacc gaaaaagggt atttccactc tccggcgacc ggacagttaa tgctcgatca
      481 cccgatggtc gctgccgacg tgcaaaatcc ccaccagccg aaaacagcaa catgggtgat
      541 tgttgaggcg ctggctcgcc gtaaagcggc aggacttccc gcattcccgc atttaccgtc
      601 atgtcatgtg acaacatgcc agaaaacggt catgttatgc gtgacgttgt cacttcctac
      661 gcgcaagccg ttgatgtaaa actggcacaa tggatcgaag ataacgtgac tttcccatca
      721 acaatggtgg accgtattgt gcccgcagtg gcagaggata cgctggcgaa aatcgaacaa
      781 cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac ctttccgcca gtgggttatc
      841 gaagataact ttgtagccgg acgtccggaa tgggaaaaag cgggagccga actggttagt
      901 gatgtgctgc cttatgaaga gatgaagttg cgcatgctca a
//
LOCUS       DAJVQH010000307          939 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJVQH010000307 DAJVQH010000000
VERSION     DAJVQH010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.313"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            14..925
                     /locus_tag="OQM87_004297"
     CDS             14..925
                     /locus_tag="OQM87_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1510155.1"
                     /translation="MINEGDKSQEDIADEAGMSPATLSALRKGEYNGNVEGMHDKLQK
                     WYQVWQKGQNLSGTPGVVKTQIFCDLHELFKTVRSLGIISVVVGVPGVGKTFAAREYC
                     RRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGGKETSGR
                     VNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDELARLFSR
                     FAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNHAWLAAK
                     GAGTSLNKSHIKAAFKEAYSNPRLLNW"
BASE COUNT          264 a          197 c          264 g          214 t
ORIGIN      
        1 cactattaac gctttgatta acgagggcga caagtctcag gaagatattg ctgatgaagc
       61 cggaatgtct ccggcaacac tctcggctct gcgtaaagga gagtacaacg gtaatgtcga
      121 aggtatgcat gacaaattac agaaatggta tcaggtctgg cagaagggac agaacttgtc
      181 aggtacccct ggtgttgtga agacccagat attttgtgat ttgcatgagc tgtttaaaac
      241 agtgcgctca ctcgggatta tcagtgtcgt tgttggcgta ccgggtgtcg gaaaaacctt
      301 tgctgcccgg gaatattgtc gcaggacagc taatgcctgg atggtgactc ttgctccggc
      361 acattcgagc gtaacggaat gcctccttga aatagcatat gcgctggaaa ttaataatcc
      421 gggaagaaat aaggggaata taacccgcgc cattcgcaga aaactgggtg gtggtaaaga
      481 aaccagtggc cgtgttaacc cccttctgat tattgatgaa gctgatcact taagtgtgga
      541 tggtctggag caattacgcg ccattcagga tgccactggc gtggggatgg ttttaattgg
      601 caacccgaaa cagatggcgg atgccacccg tcgtgggact gatgaactgg cccgtctgtt
      661 cagccgtttt gccatgacaa aacaactgcg caaagtaaaa aagaccgatg tgatggctgt
      721 agccaaagca tggggagtaa cacaggaaga tgagctggac ctgctgatga gggtagccga
      781 aaagcccggg ggattgcgtg tattaactca cacactgaat catgcgtggc tggcggcaaa
      841 aggtgcaggc acatccctga ataaaagtca cattaaagcg gcttttaagg aggcttacag
      901 caaccccagg ttactgaact ggtagtaaag gacataaac
//
LOCUS       DAJVQH010000308          939 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJVQH010000308 DAJVQH010000000
VERSION     DAJVQH010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.314"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..179
                     /locus_tag="OQM87_004298"
     CDS             <1..179
                     /locus_tag="OQM87_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510156.1"
                     /translation="GIWCFAFTACACLTGIFPKMEAFTAEWTFQLALNVATPFVLVGL
                     GLIFPLLARKANSK"
     gene            complement(180..824)
                     /locus_tag="OQM87_004299"
     CDS             complement(180..824)
                     /locus_tag="OQM87_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1510157.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          242 a          221 c          225 g          251 t
ORIGIN      
        1 tcgggatttg gtgttttgcc tttaccgcct gtgcctgttt gacggggatc ttcccgaaaa
       61 tggaagcctt cactgcagag tggaccttcc agttggcgct gaatgttgca acgccgtttg
      121 tgctggtagg attaggactg atattcccgc tgctggcacg taaagcgaat agcaaataat
      181 tattgtggtc ggtgacgctg actctggcag atctgacccg tttttacata atcgagaaat
      241 gcgcgcaagc cgcaggacat atactggcgg tttgggtaat agacttggaa gcctggacgc
      301 tctgtgatcc agtcttccag tacacattct aaacgaccag tatcaaggta ctccttgatc
      361 tgttcataga gcagataccc gataccagca cccatcaaaa cggcctccag ttcagcatca
      421 acatcatcca gaatgatatt tcctgcgaca gcaatttcca gctcctttgc aaattgccag
      481 tggaaaggtt tgccgctggg ataacgaaaa acaacacatt gatgattaag cagatcatga
      541 ggatggcggg gtttgccata tcttgcaaag tactctggcg ttgctgctac acataatttc
      601 actggtgggc cgatcgccac gctgatcata tctttttcga cgatgccact aagtcgaacg
      661 ccagcgtcaa agccctgttg gacgatatcg ataagagaat cgtcagttgt gagctctact
      721 ttaatatcag gatattcacg tgtgaaacca accagtaaag gcattaaaaa tatacgtgca
      781 gctacccggg cggcattgat tttgagcgtt cctgtcggtg tcagacgaaa atcattcatc
      841 tcatctaaca tgatctgaat ttcgtcgaaa gctgggcgca atcgttcata gagattagaa
      901 cccgcttccg ttaacgatac gcttcgggtt gtccgattg
//
LOCUS       DAJVQH010000309          929 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJVQH010000309 DAJVQH010000000
VERSION     DAJVQH010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.315"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            373..>929
                     /locus_tag="OQM87_004300"
     CDS             373..>929
                     /locus_tag="OQM87_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1510158.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          329 a          180 c          154 g          266 t
ORIGIN      
        1 gtttaagctc cattttcaaa aaaatatatc agggtttgtt actaataact tctgaaaaca
       61 gaaaaaaata gagcggtaat tctatttcgg agaggagtaa ctccatctat cctcgatacg
      121 tactgaacgt tatcgtgata tattcatcat gctttcttca ccattattcg agtataggga
      181 gagacgcaat gaccttaaat acctttttat ggctgaatta ccattctcag gaataacagc
      241 agactccttc ccatagacgg cttaggggga aagcaatacc cggagaaagg gtacaaagca
      301 aactgtttta aaattaataa gccatattct atttaaccag aattcttatc gttgataaga
      361 attctggtta taatgattaa atcaaccaat atacaggcaa tcggttctgg cattatgcat
      421 caaataaaca atatatactc gttaactcca ttatctttac ctatggaact gactccatct
      481 tgtaatgaat tttatttaaa agcctggagc gaatgggaaa agaacggtac cccaggcgag
      541 caacgcaata tcgccttcaa taggctgaaa atatgtttac aaaatcaaga ggcagaatta
      601 aatttatctg agttagattt aaaaacatta ccagatttac cgcctcagat aacaacgctg
      661 gaaataagaa aaaacctatt aacacatctc cctgatttac caccaatgct taaggtaata
      721 catgctcaat ttaatcaact ggaaagctta cctgccttac ccgagacgtt agaagagctt
      781 aatgcgggtg ataacaagat aaaagaatta ccatttcttc ctgaaaatct aactcattta
      841 cgggttcata ataaccgatt gcatattctg ccactattgc caccggaact aaaattactg
      901 gtagtttctg gaaacagatt agacagcat
//
LOCUS       DAJVQH010000310          909 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJVQH010000310 DAJVQH010000000
VERSION     DAJVQH010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.316"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..526)
                     /locus_tag="OQM87_004301"
     CDS             complement(<1..526)
                     /locus_tag="OQM87_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510159.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWD"
     gene            complement(662..>909)
                     /locus_tag="OQM87_004302"
     CDS             complement(662..>909)
                     /locus_tag="OQM87_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HCS1510160.1"
                     /translation="RKRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRK
                     PLAARQVLSRSETVLQKSLEITEEADLALRNAMARIK"
BASE COUNT          211 a          239 c          199 g          260 t
ORIGIN      
        1 agtcccagcc gcaggtattg gctttgcaac cgtcgccctg gagccagact gtaccgttaa
       61 tggttactgg cgttacgttt ccgccagggc catccatata cccgccttta ccgaacatgc
      121 cgctcagtcc gaccggtttt aatgctttgt caagcgcagc tttgtaggca gcgtcacctt
      181 cgtacaactc gtacgttgtt ggacacgctc caggttgttc agcccgggcg tttgggtcgc
      241 agctgagcgc ttgtttcacg ctcgctgctt ccacgttaaa gccagtaaac agaaccgcca
      301 gaaataacat cagggtatgt aaacgtttca ttgtaaatat tcttccgttg taaatggcgc
      361 tggaaaattt ctcatcagtg aaaccttccg gaaatttccc ccccttgtat gatgtatatc
      421 gccagtattt tatttcatcc cggatataat ttttatattt cccggcacgt gcaataacgc
      481 tgaccagaga tataaggctc attaataact ttacaggtaa ttgcataatt aaatttccag
      541 tgagtgaatt gacaggtgag gggtaaaact ttctctcagg taatatatat ctgaaaatat
      601 tttcaataac accagctagg aaaaacacca ccacaggcgg tggcgtttct ttggagcgtc
      661 tttatttaat ccgggccatc gcgtttctca gtgccaaatc tgcctcttcc gttatctcca
      721 gacttttctg caacaccgtt tctgaccggg acagtacctg acgtgctgcc agcggtttcc
      781 ggatatgctc catcgtcagc atcgtgtctt catagccctg ctgacgccag gcgtcggtat
      841 gtgccaacgt gaaactgagc agaccgccgc tgttgtcacc atcacctgca tatttcagcc
      901 ttttccggg
//
LOCUS       DAJVQH010000311          899 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJVQH010000311 DAJVQH010000000
VERSION     DAJVQH010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.317"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            77..>899
                     /locus_tag="OQM87_004303"
     CDS             77..>899
                     /locus_tag="OQM87_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCS1510161.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGGIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKAQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRP"
BASE COUNT          237 a          214 c          230 g          218 t
ORIGIN      
        1 gtgcgctgtt gtccatgtga tattgtgtac cagatgtgtg ccaaactatt atttctgagc
       61 gtaccaaata ttatttatgg ctataagcga cacaaaactg cgtactattt atggtaaacc
      121 atattcgggc ccacaagaag tggctgatgc cgatggcctc agcgtacgaa tttcaccgaa
      181 ggggggcatc cagttccagt accgctatcg ctggcatggc aagcctaatc gacttgggct
      241 tggtcgatac ccatccctgt ctttgaagga tgccagacag atcactgctg acttgcgaaa
      301 gctctatttc tcaggaacgg atccacgcac ctattttgaa gagaaggtgg agaactccat
      361 gacggtcgcc cagtgtctcg actactggtt cgacaactac gtctctacaa ctctcagaga
      421 aaaggcccag gcactttatc gatcaacggt tatgaagcgc atgcatgacg ccttccctaa
      481 tcgtccggca tcttctatca cggttaagca atgggttgac ctgcttaccg aagaagaaaa
      541 agataatcca cgccgagcaa ggcaggtgct aagtcaacta agatcagcaa ttagttggtg
      601 catgcggcgt cagttgatag atagttgcgc aattatgagc atccagccaa gggacttcgg
      661 ctcccgcgct gaggtagggg atcgggtact gtcgtatcac gaactggcta agatttggct
      721 tgctattgaa agaagccgtg cgtctacgtc aaataagctc cttcatcaga tgcttatgct
      781 gtggggggcg aggctctcag agcttaggct ggcaacaaag acagaatttg acctgctgga
      841 caacgtatgg accgtaccga aagagcatag caagatgggt aatgttatcc gccgtccaa
//
LOCUS       DAJVQH010000312          885 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJVQH010000312 DAJVQH010000000
VERSION     DAJVQH010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.318"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
BASE COUNT          244 a          215 c          156 g          270 t
ORIGIN      
        1 acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcacg taagaggcta
       61 acctacatgt gttcagcatg gattgagcct cagattaatg ttaagcgtct tgcaggacgc
      121 gtaatgttaa ctggggcttt tctctatctg ccttttggtg ttcatgcctg agacagatag
      181 cctcaagcac ccgcagtcat tctacttaac taagatttcc ctgcaaaccg tttttgtccg
      241 gcacagtaaa tatccaacta aaccaatagc gttcgctgta tttaccgcca gtattcaatg
      301 cacatgaccg ccatgaacac ccctaaaaaa agggcattta tatgtccaaa cattaatatc
      361 aaaacatcaa ttttttccat ataccttgct gtgaagatga tgggcataca tgatgcgaac
      421 aaccagaacg caacaaacaa aaactgcaat gcgtttttca ttattccccc tacaatcaat
      481 gtgcaataac atttaaacac acctcaattt ggccggacat ataaatatct aaaccagaaa
      541 aaatcactta catagcgtta caaactcttt agtctaaata ctcatcgtaa aacattcccc
      601 atacttatca gtccgttccg cgccaggtag cttattgcct tatctggcaa cctataatca
      661 ggtttccgct ttttcagttg gctggtcgtt taaccgacat agttaaccca ttaatctggt
      721 tgccggatgc tggtggattt tcgcgtttta gttgttcata aaagtgcaca gctttaacca
      781 gttcttctga tgtaaccggg actggtgggg cagtgaataa ggcctgaatt tcatagttcg
      841 gcctgtcgtt acaatcctct tttttcggta catatttcca gtcac
//
LOCUS       DAJVQH010000313          868 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJVQH010000313 DAJVQH010000000
VERSION     DAJVQH010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.319"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            564..>868
                     /locus_tag="OQM87_004304"
     CDS             564..>868
                     /locus_tag="OQM87_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCS1510162.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     "
BASE COUNT          276 a          177 c          166 g          249 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgt
//
LOCUS       DAJVQH010000314          860 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJVQH010000314 DAJVQH010000000
VERSION     DAJVQH010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.320"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..>860)
                     /locus_tag="OQM87_004305"
     CDS             complement(<1..>860)
                     /locus_tag="OQM87_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1510163.1"
                     /translation="SAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPYASLA
                     ILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGSAALG
                     VGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYRYSTE
                     GYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDV
                     ERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLSGWWG
                     NSYATYSVSQ"
BASE COUNT          222 a          221 c          197 g          220 t
ORIGIN      
        1 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccgataaag gaacagataa
       61 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
      121 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
      181 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
      241 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgttc cctccacgcg
      301 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
      361 attgagggta tagtagccct cggtggagta acgatagcca accaggcgca agctagtgtc
      421 tgtgtcgtca aatagttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
      481 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
      541 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
      601 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
      661 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtcagac cttcacgttt
      721 aagaatcgcc aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
      781 gatggttacg tccaaatcgc cacctgtact tgcggtgttg agatcggtga tctcaaaagc
      841 accttgtgga acggctgact
//
LOCUS       DAJVQH010000315          841 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJVQH010000315 DAJVQH010000000
VERSION     DAJVQH010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.321"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..>841)
                     /locus_tag="OQM87_004306"
     CDS             complement(<1..>841)
                     /locus_tag="OQM87_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HCS1510164.1"
                     /translation="LIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYINLIWA
                     WGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHHFFTMG
                     AGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFSVGGMT
                     GVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNETWGKR
                     AFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALGILCLV
                     IQM"
BASE COUNT          247 a          224 c          225 g          145 t
ORIGIN      
        1 tacatctgaa taacgaggca gagaataccc agcgcaatca gtactgcacc gctggctgca
       61 atcatcagca tggtgtggaa ctgcgggtca atctgctggc tcaaacgacg ggtcatgccc
      121 atgaagccca gcgcatacag cggcataaag gcaacgaaga agccgatgat ccaaaaccag
      181 aacgcgcgtt taccccaggt ttcgttcagt ttgaaaccga acgctttagg ccaccagtag
      241 gtcatccctg cgaagcagcc gaagaccacg ccgccgatga tcacgttatg gaagtgcgca
      301 atcaggaaca ggctgttatg cagaacgaag tccgcgcccg gtactgccag cagcacgcca
      361 gtcataccgc ccaccgagaa ggtgacgata aaaccgatag tccacagcat cgcagaatgg
      421 aacacgatgc ggccctgata catggtgaac agccagttga agatcttcac cccggtcggg
      481 atggcgataa tcattgtggt gataccaaag aaggcgttta cgttcgcgcc cgcccccatc
      541 gtaaagaagt ggtgcagcca aacgatgaac gacagcacgg tgatgcagac ggttgcccat
      601 accagcgagg tataaccaaa cagacgttta cgcgagaagg ttgccgcaat ttcggagaac
      661 acaccgaaaa caggcaggat caggatgtaa acttccgggt ggccccaggc ccaaatcagg
      721 ttgatgtaca tcatcatgtt gccacccata tcgttggtaa agaaatgggt gcccagatag
      781 cgatccaggg tcaacaacgc gacggtaacc gtcagaattg ggaaggaagc aataatcagt
      841 a
//
LOCUS       DAJVQH010000316          826 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJVQH010000316 DAJVQH010000000
VERSION     DAJVQH010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.322"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(8..328)
                     /gene="psiF"
                     /locus_tag="OQM87_004307"
     CDS             complement(8..328)
                     /gene="psiF"
                     /locus_tag="OQM87_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCS1510165.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(447..>826)
                     /locus_tag="OQM87_004308"
     CDS             complement(447..>826)
                     /locus_tag="OQM87_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCS1510166.1"
                     /translation="SIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTA
                     DHAHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHA
                     ANVVGLTDQTDLFYTMKAALGLK"
BASE COUNT          177 a          222 c          201 g          226 t
ORIGIN      
        1 tcaggtatca ggcggctttc ttgaggcagg cactcataaa cttattacga tcatcacctt
       61 tcagagattg ttgcgttgct tgattattac attcgcgcat cttttgctgc tgtggcgtca
      121 aacttttttc accaggcgca gacttgctgt tcttcaggca atcactcatg taggtcttac
      181 gagcatcccc tttcaacgcc tgcgccgtcg cctgctgatt acaggaggtc atacgctgtt
      241 gttgtggggt taaggttctc tcggcagcgc cgacggtggt taaaaaaacc agaccaaaaa
      301 gcaaggtaac cagtaatgtt attttcatag caccatccct cttcatgttt taaccatgag
      361 cgtttgcgcc cgtgatctgc cattaagtct ggttgctaac agcaaaaaaa ccacccggca
      421 gcgaaaattc actgccgggc gcggttttat ttcagcccca gagcggcttt catggtgtag
      481 aagagatcgg tctggtcggt cagtccaaca acattggcgg catgcgggcc atacgccgca
      541 atacgcaact gactgccggt atgttcttgt gaatcctctt cggaattccc gtaactcatc
      601 accatcactg cgccatcttt agtatttagc gcctgagtga ggcccggcgc tttggtatcc
      661 ggcgcaacaa cctggctggc gtgggcgtga tcagcggtga ctatgaccag cgtgttaccc
      721 tcctttttag cgaattccag cgcccgttgt acggcttcat cgagatcgac cgtctcgcca
      781 atttgcccac aaggattcgc agcgtgatcc tgtttatcga ttgacg
//
LOCUS       DAJVQH010000317          812 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJVQH010000317 DAJVQH010000000
VERSION     DAJVQH010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.323"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..366)
                     /locus_tag="OQM87_004309"
     CDS             complement(<1..366)
                     /locus_tag="OQM87_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1510167.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMISRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEG"
     gene            complement(378..779)
                     /locus_tag="OQM87_004310"
     CDS             complement(378..779)
                     /locus_tag="OQM87_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCS1510168.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVSAGVAAGMTERGLARMQ"
BASE COUNT          198 a          252 c          170 g          192 t
ORIGIN      
        1 cccttcataa tatcgtcctg cccttacgtt tcccggcagc ccctcacgcc gcaaccggat
       61 ataactacat ccgccatcat caggggtgat acgatccaca cagaaagact cacgactaat
      121 catcagcgaa tcattgcgac acagccgtaa aatatctgct gttttcacaa acagggatgg
      181 taaagagtct tcaatacgca caccgccggc caccagagaa atattttcag gatcatcaaa
      241 aaccccggaa atcatgatcc cttttagccg gcctgacgta atgaccgctc taatccccat
      301 attgcgaata atggccttgt ctgcgagatc aagtgcaaca tcaaacagat tatcgaaatc
      361 agccacgctg cctcccgtta ttgcattctg gccaggccac gttctgtcat tccggctgcc
      421 acaccggcag agacacggaa cgccattccc ggcggcacaa atgccagagg ttcatcccgc
      481 gtggcatgaa gtgcatcggt atgcagcgtc accagtgcca cgactgtaac cagatcatcc
      541 atattctgaa tcacggtctc cggctgcgct gatatcacct cattttcatg tccggtcagc
      601 gcattttccg ggtgggcaga cttatcccgg atgtcagtgt catccatatt ggcaagttcc
      661 tcctccagct cagccacacg ctgcgcaata tctgctgcgc ttcccgagat atccggctca
      721 cgtcccagcg atgctgccag ttctctcaga cgtttcaggt tatcgtcttt ctttgccata
      781 tctgttcctg tccgaataaa aaacgggagc cc
//
LOCUS       DAJVQH010000318          806 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJVQH010000318 DAJVQH010000000
VERSION     DAJVQH010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.324"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(1..639)
                     /gene="ospI"
                     /locus_tag="OQM87_004311"
     CDS             complement(1..639)
                     /gene="ospI"
                     /locus_tag="OQM87_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCS1510169.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          250 a          193 c          103 g          260 t
ORIGIN      
        1 tcagcaaagc ctcttacttt tccaactact actgttagca tagtgatcaa attcctgatc
       61 agatacatga attacctgaa ccgaactatt ttcatctaca aatgcatctg gcgatttttc
      121 tacaggatca taaagattaa atccctttcc tgtttgaaca tccaaaaaca taattttttc
      181 accatcgtta aatccagcta tccaatgagt ggcctgttca atagaaatca ttactacact
      241 tccttttgaa gtattttgta aaatattttc tgcaatacgc tcacgaaatt tactataagt
      301 ttcattagga tgtgacacta tgccttcact atcaatttgc tctaaatgcc tatgaaaaaa
      361 tccggtcata tactctgata tttcattagg tacgggagcc tctcttactc cataccctaa
      421 catagccata caagcatgaa gcgcacaccc tgaacagtta ccatccccac cagacggatt
      481 tatcattttc tgtaaagtcg ctgccaattc agaatcttta tccatatcac caagactttt
      541 agagtttaaa gattccagca attttttaac atttacattt ccctcctctg tatgtgcctc
      601 atatccagca gttccctgta acgacacccc attaatcata ttgtatcctg acagtatata
      661 tactcactat attttcttca taacaaatat ccctctgata tgacaacaaa aaacggagcc
      721 ggattcctgt ttttgtgaac tgcatctatt cgtgtctaaa aagtgatcca cttaccacca
      781 acggcctaat atatacagaa cggagt
//
LOCUS       DAJVQH010000319          795 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJVQH010000319 DAJVQH010000000
VERSION     DAJVQH010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.325"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..795
                     /locus_tag="OQM87_004312"
                     /pseudo
     CDS             1..795
                     /locus_tag="OQM87_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    279..395
                     /locus_tag="OQM87_004312"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          184 a          196 c          239 g          176 t
ORIGIN      
        1 actaaaaata ctcgtttttc ccccgaagtc cgtcaacggg cagtccgtat ggttctggaa
       61 agtcagggcg aatatgactc acaatgggcg acaatttgtt ccattgctcc aaagattggc
      121 tgtacgccgg agactctgcg tgtctgggtt cgccagcatg agcgggatac cgggggcggt
      181 gatggagggc tcaccaccgc tgaacgtcag cgtctgaaag agctggagcg tgaaaatcgt
      241 gaactgcgcc gcagtaacga tatccttcgc caggcttccg cttattttgc gaaggcggag
      301 ttcgaccgcc tctggaaaaa atgatgccac tgctggataa gctgcgtgag cagtacgggg
      361 tcggaccgct atgcagcgaa ctgcatattg ccccgtcaac gtattaccac tgtcagcaac
      421 agcgacatca tccggataaa cgcagtgccc gtgcgcagcg cgatgactgg ctgaagaaag
      481 agatacagcg cgtatacgat gaaaatcaca aggtatacgg tgtgcgtaaa gtctggcgtc
      541 agttgttacg ggaaggtatc agagtggcca gatgcactgt ggcacgtctc atggcggtta
      601 tgggacttgc cggtgttctc cggggtaaaa aggtccgtac gaccatcagc cggaaagccg
      661 ttgccgcagg cgaccgcgta aaccgtcagt tcgtggcaga acgacctgac cagctgtggg
      721 tggctgattt tacttacgtc agcacatggc agggcttcgt ctatgtggcg ttcatcattg
      781 atgtgtttgc cggat
//
LOCUS       DAJVQH010000320          790 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJVQH010000320 DAJVQH010000000
VERSION     DAJVQH010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.326"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(144..>790)
                     /locus_tag="OQM87_004313"
     CDS             complement(144..>790)
                     /locus_tag="OQM87_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1510170.1"
                     /translation="SIYYDGEQVRVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQY
                     VEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFL
                     DKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDY
                     LFGSFTSNSSLILYKPEFERKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
BASE COUNT          200 a          204 c          163 g          223 t
ORIGIN      
        1 tctcggcttt gttcagcttt cagcgacacc aaatgctttt ggtgctgtac ctatgttttt
       61 ggcgattcag catattctcg cggcttagat tattatcgcc acccgccagg gcaagttttg
      121 ccttagccgg gggctgagca gcgttaccac ttttgttcaa ttgcccccgc tttcagcaac
      181 tccacatcct tgccgttaat agttgccgtt gcggcaacta ttgtggtcct tacagttgca
      241 caagattttc gctcaaattc cggtttataa agaataagag acgaattact ggtaaatgaa
      301 ccaaataggt aatcagtaaa gctaatattc agccggttta aaccgaaagg cgcgaagcca
      361 accgtttggt taatctcctt accggatggg cgacgaatgc tgaatgtcac gcgaagattg
      421 gccacttcgc ctaatttatt ggtggtggcg aagaggctat ccatgcagga aaaaccttta
      481 tctaaaaaat tatcccgcat cccagaacgg gtaaccgtga tcacgggtaa cgtatcctca
      541 accgtttttc tgtcaatcat gtcttttcga caagtagcca gaaagtcttc cgggccacca
      601 taaatgtgga cacctttatc aaggtattcc ttcgctttct tacaatctac ctcaacatac
      661 tgaccattgg cgtacatcat gcccaacctg gaccatgcta aattcatacc ttgctgcgcg
      721 gcctgattaa atagtgcata cgctttggca taatccaccc gcacctgctc accgtcgtag
      781 tagatactcc
//
LOCUS       DAJVQH010000321          780 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJVQH010000321 DAJVQH010000000
VERSION     DAJVQH010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.327"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..259)
                     /locus_tag="OQM87_004314"
     CDS             complement(<1..259)
                     /locus_tag="OQM87_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510171.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWL"
     gene            complement(261..539)
                     /locus_tag="OQM87_004315"
     CDS             complement(261..539)
                     /locus_tag="OQM87_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510172.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDSCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            480..665
                     /locus_tag="OQM87_004316"
     CDS             480..665
                     /locus_tag="OQM87_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510173.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(707..>780)
                     /gene="hokD"
                     /locus_tag="OQM87_004317"
     CDS             complement(707..>780)
                     /gene="hokD"
                     /locus_tag="OQM87_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1510174.1"
                     /translation="LCEVRIRTGQTEVAVFTAYEPEE"
BASE COUNT          177 a          210 c          226 g          167 t
ORIGIN      
        1 acagccagtc agacaatgcg ccagcaccac cagcagcacg aatcacccgt gcgttactga
       61 aaaacggcag caatgttttg tcttccacta gcggctggcg aacggcagga acgaccccgg
      121 acggcagatt acgcatgctt ttcggttccg gctccaccag tacccgggta ttgtggaata
      181 ccggcatgga ttcacggcct ggcttaagga ccaccagccc gagttccgga accagaacag
      241 gtcgaagtaa tacccgcaca ttacctccag atccgttgct ggaatgtgca ggacggacgc
      301 ggtggccgtt cggaataagg gagcctgacg aagattatcc agtgacgata atcgagactg
      361 agggctttct taatctcgta tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt
      421 tcttcggtgc aggagtcgtg ctgataccag tcagatttga atgcatgaga acgccgcccg
      481 tgcctgctgg caggggcggc agagttatcc gaattgtaaa atttggtatc gtgcgccatc
      541 tgttttctct gctggcgcag caggtgccag ttgttcaggc tgacggatgg attgtaaacc
      601 agaacgacca gaaaaaacaa aacccgccga agcgggttaa gtgcgggtgc gttgaggatg
      661 cctgacacat cagcggtggc gagggatttc tcccccgccg ggtctcttac tcctcaggtt
      721 cgtaagctgt gaagacagcg acctccgtct ggccggttcg gattcgtacc tcgcagaggt
//
LOCUS       DAJVQH010000322          761 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJVQH010000322 DAJVQH010000000
VERSION     DAJVQH010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.328"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>761
                     /locus_tag="OQM87_004318"
     CDS             <1..>761
                     /locus_tag="OQM87_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1510175.1"
                     /translation="RILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFELD
                     LEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGADSWAGTD
                     LFMTNRTNGVATYRNTDFFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDG
                     FSFAGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDV
                     VNKTQNFEAVLQYQFDFGLRPSIGYSYSKALDVAG"
BASE COUNT          211 a          156 c          184 g          210 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcgctgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta
      421 caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg
      481 gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg
      541 gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt
      601 atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga
      661 acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat
      721 ccatcggtta ctcctattct aaagcactgg acgtagcagg t
//
LOCUS       DAJVQH010000323          761 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJVQH010000323 DAJVQH010000000
VERSION     DAJVQH010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.329"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..726
                     /locus_tag="OQM87_004319"
                     /pseudo
     CDS             1..726
                     /locus_tag="OQM87_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
BASE COUNT          191 a          186 c          219 g          165 t
ORIGIN      
        1 ctccatctgg aacatattca gcctgtccgc aatttattaa tcacactgca ggaaaaactc
       61 gccaccccgg caggacagca ggtcgcacag accctgcagc aaaccgggga tacccgcgaa
      121 ctgctggata ttctgacgga tgatggctgg ctcaaaaatg aataccacgg ggaggaggga
      181 atctttaccg gactggcatc gttaaacgat ctggttcgtc ttgctgcggc aatggggagt
      241 gaatttcctt ttgatgaata cgcagaagtg cagaaactgc cggtgatcga cgtggaattc
      301 agccatctgg tggggatgga tgaatgccag ggaacactca cactggtgga tacccccggc
      361 cctaatgaag ccggacaacc gcagatggaa gtgatgatgc gggatcaact gcagaaagcc
      421 tctgcggtgc tggcggtgat ggactacacc cagatgaact cgaaagccga tgaggaggtt
      481 cgtaaagagc ttaatgccat tgctgatgtg tcagtcggcc gcctgtttgt gctggtcaat
      541 aaatttgatg aaaaagaccg taatggcgat ggggctgatg cagtctgtca gaaggtaccg
      601 gcaatgctga acagcgatgt gctgcccgcc tcccgcgttt atcccggatc ctcacgccag
      661 gcatacctgg ctaaccgtgc gcttcatgag ttacggaaaa acggaaccct tcctgttgat
      721 gaagcctagg tcgatgattt tgtcagggaa gccttcggtc g
//
LOCUS       DAJVQH010000324          755 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJVQH010000324 DAJVQH010000000
VERSION     DAJVQH010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.330"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..147
                     /locus_tag="OQM87_004320"
                     /pseudo
     CDS             1..147
                     /locus_tag="OQM87_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
     gene            144..>755
                     /locus_tag="OQM87_004321"
     CDS             144..>755
                     /locus_tag="OQM87_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCS1510176.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIATSPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLAS"
BASE COUNT          170 a          188 c          219 g          178 t
ORIGIN      
        1 gactagtgca gtgaagagca gcatccggtg atctggtcaa ccacgcaggc tgcagaacac
       61 ttaccggttc gcatgcctcg cctctgctct gcagaacgcc tctctgaaag tatggtcgat
      121 gaaatatttc agaagggggc tgcatgagta cagaaatgaa aacggggctg gtgctgtccg
      181 gtggcggggc ggtgggcgct tatcaggcgg gagtggttaa ggcactggca gagtgtgata
      241 cacagatcag catggtttca ggggccagca ttggcgcatt caatggtgcc attatcgcga
      301 cctctcccga tctgtcagaa gctgccgtac gcctggaagc gctctgggat catctgggga
      361 ataatcaggt gctgtcggta aaccgattgg tttacttttc attgctgaaa aaattgttcc
      421 aggcaatgaa cctctgccag atccccggac gtgcaggagc actgcttacg acgcttcttc
      481 gccatatatc gatactcaac gggtttgaca atctgatggc tcagccgttg ttgtcagatg
      541 agcccctgac agcgctgttg gatcattatc ttgatactga tgctctggca gacgggctac
      601 cgctgtatgt gtcgctgtac cccacagaag ggggcatgca ggatattatt gactgcattc
      661 gtgctgaact gggtgccgga accacgaaaa acgccgtttt tcagcatatc cagagcctgc
      721 cccgcggaca gcagaaagag gctctgcttg cgtca
//
LOCUS       DAJVQH010000325          753 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJVQH010000325 DAJVQH010000000
VERSION     DAJVQH010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.331"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..753
                     /locus_tag="OQM87_004322"
                     /pseudo
     CDS             1..753
                     /locus_tag="OQM87_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          217 a          181 c          202 g          153 t
ORIGIN      
        1 atgaagctcg ccttgagcga gatatattaa aaaaagcaac agcgtatttt gcacaggagt
       61 cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa tttcccattg aagcgatgtg
      121 tcaggtattt ggtgtatcca ggagcggtta ttacaactgg gtacagcatg aaccctcaga
      181 cagaaaacaa agtgatgagc ggctaaaact ggagattaag gtggcacata tccgcactcg
      241 cgaaacatat ggaacccggc ggctccagac ggagctggca gagaatggca tcatcgttgg
      301 tcgtgaccga ctggcacgtc ttcgtaagga gctgaggcta cgctgtaagc agaaacgcaa
      361 gttcagagcg actacgaacc cgaaccacaa tctgccagtt gcgccaaatc tgctgaacca
      421 gacgttcgct cctacagcac caaatcaggt ctgggtggcg gacctgacgt atgttgccac
      481 acaggaggga tggttgtacc tcgctggcat caaagatgtt tatacgtgcg aaattgtcgg
      541 ctacgccatg ggagagcgca tgacaaaaga gctgacaggt aaagccctgt ttatggcgct
      601 caggagccag cgcccacctg ccgggctaat ccaccactct gatcgaggtt cacagtactg
      661 cgcatacgat taccgggtca tacaggagca gtttggtctg aaaacatcaa tgtcgcgtaa
      721 aggtaactgt tacgacaacg ctccgatgga aag
//
LOCUS       DAJVQH010000326          737 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJVQH010000326 DAJVQH010000000
VERSION     DAJVQH010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.332"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(559..>737)
                     /locus_tag="OQM87_004323"
     CDS             complement(559..>737)
                     /locus_tag="OQM87_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1510177.1"
                     /translation="YKDSDRLNYIEIGTWYYFNKNMNIYTAYQINLLDKSDYVLAHGL
                     NTDDQLAVGIVYQF"
BASE COUNT          202 a          118 c          190 g          227 t
ORIGIN      
        1 actatatttg cgactgagct ggtcgctatt tttaaccagg ctgaaaaatt gagtcaatat
       61 atcgaaagcg gtagttggcg ggcataagta tgtaatgatg attctgcact ggtgttgaat
      121 attattcacg ccaaactttc ctcattgagc aatgtcgtcg caagtggcct caatgatttt
      181 agaccattat cagaggtccg gagcggcggg aagagattta ttgctgtgga ataattgtgg
      241 tactgatgat tatttatatc ccggattggt agggaaaagc ttttttcatg cactattttt
      301 gcaaatattt ctatatggga agcggttttg ttacagatta aatttataaa gaaatgcatt
      361 tggggtgttg ggttgaagag tagctggtag atgttattgt gccagcaggg gcaggggaat
      421 gtgccgtatc gatatgcttt ggcaattgcg tgtgccgcag atggagatga aatgccaata
      481 aaaaagcctg agttcgatga gggacgaact caggctggtg ttatgtttta cagtaacacg
      541 cctcggcgcg ttggctgatt agaactggta gacgatacca actgccagtt ggtcgtcggt
      601 gttcagtcca tgagcgagaa cataatcact cttatcaagc aggttgatct gataagcggt
      661 atatatgttc atattcttat tgaagtaata ccaggtaccg atttcaatat agttcagacg
      721 atcagaatct ttgtaac
//
LOCUS       DAJVQH010000327          736 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJVQH010000327 DAJVQH010000000
VERSION     DAJVQH010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.333"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>736
                     /locus_tag="OQM87_004324"
     CDS             <1..>736
                     /locus_tag="OQM87_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1510178.1"
                     /translation="FRLFCEGGVRPPLEKMMPLLDKLREQYGVRPVCSELHIAPSTYY
                     HCQQQRHHPDKRSARAQHDDWLKKEIQRVYDENHQVYGVRKVWRQLLREGIRVARCTV
                     ARLMAVMGLAGVLRGKKVRTTVSRKTVATGDRVNRQFVAERPDQLWVADFTYVSTWQG
                     FVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSL
                     AYTERLKEAGLLASTGSTGDSYDNAMA"
     misc_feature    6..122
                     /locus_tag="OQM87_004324"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          171 a          176 c          224 g          165 t
ORIGIN      
        1 gcttccgctt attttgtgaa ggaggagttc gaccgcctct ggaaaaaatg atgccactgc
       61 tggataagct gcgtgagcag tacggagtca gaccggtatg cagcgaactg catattgccc
      121 cgtcaacgta ttaccattgt cagcaacagc gacatcatcc ggataaacgc agtgcccgtg
      181 cgcagcacga tgactggctg aagaaagaga tacagcgcgt atacgatgaa aatcatcagg
      241 tgtacggtgt gcgtaaagtc tggcgtcagt tgttacggga aggaatcagg gtggccagat
      301 gtacagtggc gcgcctcatg gcggttatgg gacttgccgg tgttctccgg ggtaaaaagg
      361 tccgcactac cgtcagccgg aaaaccgttg ccacaggtga ccgcgtaaac cgtcagttcg
      421 tggcagaacg tcctgaccag ctgtgggtgg ctgattttac ttacgtcagc acatggcagg
      481 gcttcgtcta tgtggcgttt atcattgatg tgtttgccgg atacatcgtg gggtggcggg
      541 tctcatcgtc tatggaaacg acattcgtgc tggatgcgct ggagcaggcg ttgtgggccc
      601 gtcgtccgtc tggcaccatc catcacagcg ataaaggctc tcagtatgtg tcactggcct
      661 atacggagcg actaaaagaa gccggattac tggcatcaac agggagtaca ggcgactcgt
      721 atgacaacgc gatggc
//
LOCUS       DAJVQH010000328          735 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJVQH010000328 DAJVQH010000000
VERSION     DAJVQH010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.334"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            85..>735
                     /locus_tag="OQM87_004325"
     CDS             85..>735
                     /locus_tag="OQM87_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCS1510179.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTAHWVVMKHIFHRLDSEALLFVMRTRSEQK"
BASE COUNT          207 a          143 c          209 g          176 t
ORIGIN      
        1 gcagtttgat gaaaccaata ttgtgcgcgg actgcctgtt cctggtggtc ggttggggat
       61 aaaaatcaca gagctggagt gtgcatgaat aaccagtatt tacagtttgt tcgtgagcag
      121 ctcattatcg ccaccgctga tttgagtggg gcgacaaaag gtcagcttga agcctggcag
      181 gagaatgcca tgttcgatac agggcgttac aggcgtaaaa aaatccggta ccgcgatgag
      241 gtgaccggaa aaatgataac gcgggataat ccaccaatcc cgggaaaaca atcactggcg
      301 aagggggcgt caattcctct ggtcagtcag gttgcgtttt cgacatcatc gtggcggcgg
      361 gctgttctgt ctcttgaaga gcaccataaa gcctggttgt tgtggtgtta cagcggaagt
      421 atttgctggg agtatcagat caccataacc cactgggcgt gggaagagtt taaggctcat
      481 tctggcacta ggaaaattgc agagaaaaca caggaacgcc tgaaaaaatt aatctggctg
      541 gcggcgcagg cggtaaaagc agaacttttt ggtggggaag gttatgaata tcaggagctg
      601 gcattactgg cgggagtaac aaccaaaaac tggtccaaaa catttactgc tcactgggtt
      661 gtaatgaaac acatttttca ccgactggat agtgaggctt tattgtttgt gatgagaaca
      721 cgttcagaac aaaaa
//
LOCUS       DAJVQH010000329          718 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJVQH010000329 DAJVQH010000000
VERSION     DAJVQH010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.335"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..153
                     /locus_tag="OQM87_004326"
     CDS             <1..153
                     /locus_tag="OQM87_004326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510180.1"
                     /translation="FVRVDDEGYPEIARCTEREFATILAGISAGGMYCPECGTVHWPD
                     GVAPPF"
     gene            153..>718
                     /locus_tag="OQM87_004327"
     CDS             153..>718
                     /locus_tag="OQM87_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1510181.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSM"
BASE COUNT          149 a          189 c          216 g          164 t
ORIGIN      
        1 tttgtacgtg ttgatgacga agggtatccg gaaatcgccc gctgcacgga gcgggaattt
       61 gcgaccattc ttgccggtat cagcgccggc ggtatgtact gcccggagtg tggcacggtt
      121 cactggccgg acggagtcgc cccgcccttc tgatgcttcc ccgttttgcc gatatttttc
      181 agcagggaaa ccgctggctt aactggctgg agaaacaacc ggaaggggca gtccgtccgg
      241 tggtaattga atcagtgaca aaaatcatgg cctgcgggac cacactgatg ggctacacgc
      301 agtggtgttg ttcatcaccg gactgttgcc atacaaaaaa ggtctgcttc cggtgtaaaa
      361 gccgctcctg cccgcactgc ggggtgaagg ctggcgcaca gtggatacag tatctgctga
      421 gcctggtccc cgactgcccg tggcagcata ttgtgttcac acttccctgc cagtactggt
      481 ccctggtgtt ccacaaccgg tggttactgg cagagatgag ccgcattgca gcggatgtga
      541 tactggaaat ctgccatcag gcagatgtgg agccggggat attcacggtg atccacacat
      601 gggggcgtga ccagcagtgg catccgcata tccatttatc gacaactgcc ggtggtgtga
      661 cgtcgggcca cacctggaaa aatcttcatt tttacgcccg taaggtgatg agcatgtg
//
LOCUS       DAJVQH010000330          716 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJVQH010000330 DAJVQH010000000
VERSION     DAJVQH010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.336"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..449)
                     /locus_tag="OQM87_004328"
     CDS             complement(<1..449)
                     /locus_tag="OQM87_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1510182.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSKLREAVATERLTVDQHRYINDVIGVLKACQDRLNG"
     gene            complement(584..658)
                     /locus_tag="OQM87_004329"
     tRNA            complement(584..658)
                     /locus_tag="OQM87_004329"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(623..625),aa:Gly,seq:tcc)
BASE COUNT          184 a          158 c          187 g          187 t
ORIGIN      
        1 ccattgagtc tatcctgaca ggccttaagc acaccgataa cgtcattaat atagcgatgc
       61 tggtctacag tcagacgctc ggtcgctaca gcttcccgaa gtttggactt aatgtcatcg
      121 tactggtcaa cgtaaagctg gtaggactcc gggtcgttca ggtcatcacg gctgacacta
      181 agttcccact cgacaatggt cttcaggtgc tcccgtagct cctcataaga ggcgttaggg
      241 ttgtctgcat ggattgcttt aagtgctgac ttgatgtgcc gtgagtgagc catagcggta
      301 cgtctatcct tggtccgtaa agatactgac gctagtttat aacggttgtg agaaggaagc
      361 gaagcggtca ggcgactagt ggtcttcatc ctaagacgga tgtaaaagac accattacgc
      421 tgataaaggt aggttcttga cttgagcatg tactgagacc ttaggtctgc tcactacttc
      481 aacaaagtta gtcacagtgt tggttacagt gagtaaaact atcggaaggc gttaagccgt
      541 atcccttagg attcagtaag ttagtagact ttaagaagtg tcatggagcg ggcgaaggga
      601 atcgaacccg catcatcagc ttggaaggct gaggtaatag ccattatacg atgcccgcat
      661 atggtgccga ctaccggaat cgaactggtg acctactgat tacaagtcag ttgctc
//
LOCUS       DAJVQH010000331          713 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJVQH010000331 DAJVQH010000000
VERSION     DAJVQH010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.337"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            157..>713
                     /locus_tag="OQM87_004330"
     CDS             157..>713
                     /locus_tag="OQM87_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1510183.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLMVSGNRLDS"
BASE COUNT          251 a          131 c          129 g          202 t
ORIGIN      
        1 gggtctggct ggctaacacc ccattataag gggtacgaat aaagtaacgg gggaagcaat
       61 acctggagaa agagtacaaa gcaaactgta ttaaacttaa taagttatat tgtatttcac
      121 tagagttctt atcgttgata agaaattctg gttataatga ttaaatcaac aaatatacag
      181 gtaatcggtt ctggtattat gcatcaaata aacaacatac actcgttaac tctattttct
      241 ttgcctgtga gtttgagtcc atcttgtaat gaatattatt taaaagtctg gagtgaatgg
      301 gaaaagaacg gtaccccagg cgagcaacgc aatatcgcct tcaataggct aaaaatatgt
      361 ttgcaaaatc aaggggcgga attaaatcta tctgagttag atttaaaaac attacctgat
      421 ttaccgcctc agataacaac actggaaata agaaaaaacc tattaacata tctccctgat
      481 ttcccaccaa tgcttaaggt aatacatgct caatttaatc aactggaaag cttacctgcc
      541 ttgcccgaga cgttagaaga gcttaatgtg ggtgataaca agataaaaga attaccatat
      601 cttcctgaaa ctctaactca tttacggatc cataataacc gattgcatat tctgccacta
      661 ttgccaccgg agctaaaatt actgatggtt tctggaaaca gattagacag cat
//
LOCUS       DAJVQH010000332          700 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJVQH010000332 DAJVQH010000000
VERSION     DAJVQH010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.338"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..479
                     /locus_tag="OQM87_004331"
                     /pseudo
     CDS             1..479
                     /locus_tag="OQM87_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108504.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            519..>700
                     /locus_tag="OQM87_004332"
     CDS             519..>700
                     /locus_tag="OQM87_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1510184.1"
                     /translation="MDVYANGQHVFRFQNGVAIAFKNIQAGTARKFTLSSANNSTKNA
                     AFYLWGNPSRPVVAELG"
BASE COUNT          187 a          148 c          182 g          183 t
ORIGIN      
        1 ctactgcggc agacaggttt ccgtatttta cggggaatga tgtcgccagc ctggcaaccc
       61 tgacaaaagt cgggcgggat attcttgcga aatcgaccgt tgccgccgtt atcgaatacc
      121 tcggtttaca ggaaacggta aacaaggctg ataacgctgt ccaaaagaca ggcgatacct
      181 tgtccggtgg acttactttt gaaaacgact caatccttgc ctggattcgg aatactgact
      241 gggcgaagat tggatttaaa aatgattcgg atgcagacac cgattcctac atgtggtttg
      301 aaacaggtga caacggcaat gagtatttca aatggagaca ccgcctcgct ggcggccagc
      361 tcaaagagct gatgaatctt aaatgggatt cactaaatat tctggttaat gccgtcatta
      421 atggttgcct tggcattggt acgacgaatg cgttaggtgg aaattcaatt gctttcgggg
      481 ataacgatac cggcctgaaa cagaacggcg atggtctgct ggatgtttat gcgaatggac
      541 agcatgtatt tcgttttcag aatggcgtgg ctattgcttt taagaatatt caggccggaa
      601 ctgccagaaa attcacgtta tccagcgcca acaactccac gaaaaatgca gcgttttatt
      661 tgtggggtaa tccatccagg cctgttgttg cagagcttgg
//
LOCUS       DAJVQH010000333          692 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJVQH010000333 DAJVQH010000000
VERSION     DAJVQH010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.339"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            36..>692
                     /gene="istA"
                     /locus_tag="OQM87_004333"
     CDS             36..>692
                     /gene="istA"
                     /locus_tag="OQM87_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCS1510185.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHHNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     P"
BASE COUNT          160 a          177 c          182 g          173 t
ORIGIN      
        1 agggggtaat ctgctctcct gattcaggag agcttatggt cacttttgag acagttatgg
       61 aaattaaaat cctgcacaag cagggaatga gtagccgggc gattgccaga gaactggggc
      121 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
      181 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      241 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      301 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      361 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      421 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      481 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      541 ataatgcgtt ccgcttcttt ggtggtgtgc cgcgcgaagt gttgtatgac aatatgaaaa
      601 ctgtggttct gcaacgtgac gcatatcaga ccggtcagca ccggttccat ccttcgctgt
      661 ggcagttcgg caaggagatg ggcttctctc cc
//
LOCUS       DAJVQH010000334          684 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJVQH010000334 DAJVQH010000000
VERSION     DAJVQH010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.340"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            440..>684
                     /locus_tag="OQM87_004334"
     CDS             440..>684
                     /locus_tag="OQM87_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCS1510186.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHE"
BASE COUNT          191 a          121 c          159 g          213 t
ORIGIN      
        1 cgtgccacgg ctggctgatg gatgttcgat agcgcgagtt tgaatgaaaa tcagccggag
       61 atgattttac ataattgcta cggaattatt caatacagga attgcttgcg tctgcatgga
      121 ttgacctgaa atattcccga aaatttctct aaaaaactcg aaaaaaatgg taactggttg
      181 aatgtattaa tatgcaatgg tacgtgtcag ggattaaaag atgaacgtaa atttattcaa
      241 cgcattaatt ttaaagggtt ttattgtttg ttgacgaaaa caggaatcat gttcggtctc
      301 tttttatctg ttaaaagcca gaagcatttc cttcgctgac tttatagtca accataacac
      361 acactctact gtctgagtcc agcgtttttt aacattcttg ttaagattat gtgatcttta
      421 gcgcgggagg aaaatattga tgaaacagcc tgcgcccgtt tatcagagaa ttgcgggtca
      481 tcaatggcga catatctggc tttctggcga tatacacggt tgtcttgagc agttgcgccg
      541 caaattatgg cattgtcgtt ttgatccgtg gcgagattta cttatctcag tgggagacgt
      601 tatcgatcgt gggccgcaaa gtttacgttg tctgcagtta ctggaacaac attgggttcg
      661 tgcggtaaga ggcaatcatg aaca
//
LOCUS       DAJVQH010000335          673 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJVQH010000335 DAJVQH010000000
VERSION     DAJVQH010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.341"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            67..496
                     /locus_tag="OQM87_004335"
                     /pseudo
     CDS             67..496
                     /locus_tag="OQM87_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          174 a          127 c          213 g          159 t
ORIGIN      
        1 cgttattccg cctttctgat gcttccccgt tttgccgata tttttcagca gggaaaccga
       61 tggcttaact ggctggagaa gcagccggtg cagatgagcc gcctggaaca ttatgccggt
      121 caggatgaaa ttggcctgcg ttacaacagc caccggacaa aacgagaaga aaaccttgtg
      181 atgagtggcg acgagtttat ggaaaggttc tcctggcatg tggcggataa ggggttccgg
      241 atggtgatac ggggtcctga gtccggtgaa gcggcgatta ctggaagatg tggtgtacgc
      301 cataacggag acagtgagaa aaacggcgag gcaaatcaca aggagaggga tgtatcagca
      361 gtgactgaag gttgacccgc tgaagtgcga gctgcgcgga ggtcagatgc ggtttacggg
      421 gctgaagagg ggttaccgtc tggcagagca ggttatgatg catgagccgc tggctcgaat
      481 acggtggtgc ggctgagagt cgcatagggg aagttgcgtc catgttgagg ggaacggagc
      541 aaaaaaccat cagagataca ttgaatcaat caatgtaagc aatattaagt gtctataccg
      601 attacttgat gatcaggttg tgttgaataa acgcgcattc ttcggtgctt ttttaatctt
      661 ttggggattt aaa
//
LOCUS       DAJVQH010000336          663 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJVQH010000336 DAJVQH010000000
VERSION     DAJVQH010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.342"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>663
                     /locus_tag="OQM87_004336"
     rRNA            <1..>663
                     /locus_tag="OQM87_004336"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          154 a          146 c          222 g          141 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccgagatgag ttctccctga ccctttaagg gtcctgaagg aacgttgaag acgacgacgt
      601 tgataggccg ggtgtgtaag cgcagcgatg cgttgagcta accggtacta atgaaccgtg
      661 agg
//
LOCUS       DAJVQH010000337          661 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJVQH010000337 DAJVQH010000000
VERSION     DAJVQH010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.343"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(105..235)
                     /locus_tag="OQM87_004337"
                     /pseudo
     CDS             complement(105..235)
                     /locus_tag="OQM87_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(233..328)
                     /locus_tag="OQM87_004338"
                     /pseudo
     CDS             complement(233..328)
                     /locus_tag="OQM87_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          189 a          123 c          159 g          190 t
ORIGIN      
        1 cttccttatg ttatattttg gtaaaaaatt tattttttta cattatagtc ggctaaatga
       61 agtttactgt aaaaaaacga tactttttcg ggtggcgctt ccaatcagta gaatatgtgt
      121 ttttcaatga aggagccgat gacttttcgt ggatttccac ggaacgcgag aagcagattg
      181 ttttacgtgc cagccgcttg atgcgggtgc ggagggtcag gttgttacgt tcaagatata
      241 gcatgtgctg gatgaagatc ccttttctgg tcacctgttt atctttcgtg gccgacgggg
      301 tgacactgtt aaaatgtaat gaacgcatcc cacacaatta aacacggagt agtatgatgg
      361 ttaaaagggt gaaactggca ggtgtggagc ctcgcagctt tccggcatca tactgcccaa
      421 tacgggatgc ataaagcgtc ctcagactgg agactccaca aatgaaatat acaccggttg
      481 gcgttgatat ggcaaaacat gtcattcaga ttcacttcat caatgagcac atgtcagcgc
      541 cagtgatata agacggtaat ggggatgcgg acgatttcct ggactctcag aagatgaaac
      601 atgtttactg aagaacaaaa aattcgcgct attgagcttt atttcaaata tggtaagaag
      661 c
//
LOCUS       DAJVQH010000338          660 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJVQH010000338 DAJVQH010000000
VERSION     DAJVQH010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.344"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            31..291
                     /locus_tag="OQM87_004339"
     CDS             31..291
                     /locus_tag="OQM87_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCS1510187.1"
                     /translation="MAWARHYQQIVREEKETELADDLEKGLPQHLFESLCIDHLQRHG
                     ANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            297..>660
                     /locus_tag="OQM87_004340"
     CDS             297..>660
                     /locus_tag="OQM87_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1510188.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRR"
BASE COUNT          189 a          152 c          165 g          154 t
ORIGIN      
        1 gcttatccga tttttgatcg tattgaagaa atggcatggg ctcgtcacta ccagcagatc
       61 gttcgtgaag aaaaagaaac ggaactggcg gacgacctag aaaaaggtct gcctcagcac
      121 ctgtttgaat cgctctgcat cgaccatttg caacgccacg gtgccaataa acaggcaatc
      181 agccacgcat ttgatgatga tgttgagttt caggagcgca tggcagaaca catccggtac
      241 atggctgaaa ccattgccca ccaccaggtt gatattgatt tggaggacta aaatctatgg
      301 cgctttttca acgagcaata aacacacagg cctatctaaa agcaggaatt atggggtttg
      361 ctggcgacgg caaaacctac acagcaagcg aactggctat tggtctggta atgcttatgc
      421 ggcaacgggg aattgaagct ggtaaccgcc cggtaatgtt tctggacaca gaaacaggct
      481 ctgactgggt taaaccccgc tttgatgcgg aaaatatcga attatttacg gcaaaaaccc
      541 gctcgtttgt tgatctgctt gaagccatta atgaagcgga aagcagcggt tccgtaatga
      601 tcatcgactc catcagtcac ttctggaccg gattatgcga cgagtatgcc agacgccgaa
//
LOCUS       DAJVQH010000339          644 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJVQH010000339 DAJVQH010000000
VERSION     DAJVQH010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.345"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>644
                     /locus_tag="OQM87_004341"
     CDS             <1..>644
                     /locus_tag="OQM87_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1510189.1"
                     /translation="KTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDL
                     AAQTTQASNRIRGLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAA
                     QLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALE
                     VEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTR"
BASE COUNT          128 a          221 c          168 g          127 t
ORIGIN      
        1 taaaactgat gctcgtgacg ctgccatcat tgccgaagct gcccgtaccc tgcctcacgc
       61 gctacgcacg ctgaaactgg ctgacgagca aatcgccgaa ctctccatgc tctgcggctt
      121 cgatgatgat cttgccgcac agacaacgca ggccagcaac cgtatccgcg gccttctgac
      181 ccagatacat ccggcactgg agcgcgttct cggtccgaga cttgagcacc cggcggtact
      241 cgatcttctc cagcgatatc cctcaccaga aaaactcgct tcgctgggtg agaagaagct
      301 ggcagcccag ctctgcaaac ttgcgcctcg tctgggtaaa cgccttgcag cagacatagc
      361 tcaggcactg gccgaacaaa ccgtcgtcgt tcccggcacg aatgccgctg ccgtagtact
      421 gccacgtctg gcactccagc tcatcacgct gcgtaagcaa agagacgagg tggcgcttga
      481 ggtagaacag cgagttcttg ctcaccctct ttacccggtc ctgaccagta tgcccggagt
      541 cggtgtcagg accgcagcca gactcctcac cgaggtcgcc tgccgcgcct tcgcctctgc
      601 cgcacatctc gctgcttatg ctggccttgc gccggtaact cggc
//
LOCUS       DAJVQH010000340          635 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJVQH010000340 DAJVQH010000000
VERSION     DAJVQH010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.346"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(152..634)
                     /locus_tag="OQM87_004342"
     CDS             complement(152..634)
                     /locus_tag="OQM87_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCS1510190.1"
                     /translation="MAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKELRVAL
                     NYSQNPVSPKARSLILWTERGAARHAKMLETDQAWDVFEKLEDCYFSQRDPSTPVSCQ
                     KSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVERLMQL
                     "
BASE COUNT          165 a          158 c          139 g          173 t
ORIGIN      
        1 cgtttaacgc cctgtgccat ttttcaggca caaaaaaacc cgcataaagc gggttctttc
       61 aagtgtccat gtctgctatt cgcctcgcga tacagctttg cgaagcttac cggaattgaa
      121 gcagtttatg tgcaaaaatg caagaacttt tttaaagctg catcaacctt tccaccagtt
      181 tatctctgcg aacaacaaac caaccattgg ctctcgccag ttccagccat gactcaaggg
      241 aaataacaat atcatcatcc cgtaactgaa ttgtggaaac agtgacaccg cctctctgat
      301 aacagagaac tcgcgtgtcg taacttttct ggcatgaaac tggcgttgac ggatcccttt
      361 gactgaaata gcagtcttcc agtttttcga acacatccca cgcctgatcg gtttcgagca
      421 ttttggcgtg gcgtgctgcg cctcgttctg tccagaggat gagggagcgg gctttgggtg
      481 aaactggatt ttgtgagtag tttaaagcga cccgcaattc tttaaggtca ttaccaacaa
      541 ctttgaaaaa gtgtttccct tcaacgaagc gtactttgtt ctcatgatga ttctggcgaa
      601 tacgcaccgg ctcagtgccg taaagctgcg ccaaa
//
LOCUS       DAJVQH010000341          627 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJVQH010000341 DAJVQH010000000
VERSION     DAJVQH010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.347"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..452
                     /locus_tag="OQM87_004343"
     CDS             <1..452
                     /locus_tag="OQM87_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1510191.1"
                     /translation="NRKKELTEPQIREGSWRKERRTSYSREFKVRLAKQALQPGAVVA
                     RIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNK
                     HDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            449..>627
                     /gene="tnpB"
                     /locus_tag="OQM87_004344"
     CDS             449..>627
                     /gene="tnpB"
                     /locus_tag="OQM87_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1510192.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWA"
BASE COUNT          170 a          156 c          175 g          126 t
ORIGIN      
        1 ccaacagaaa gaaggaatta acagagcctc aaatcagaga aggctcatgg cgaaaagaac
       61 ggcgaaccag ctatagccgt gaatttaagg tccgtctggc taagcaggcg ttacagcctg
      121 gggctgttgt tgcccggatc gccagagaac acgatatcaa taataacctg ctgtttaaat
      181 ggaaaagcca gtacgaggac ggcttactga gcgatgatga catacaggaa tgcatgcctg
      241 tcccggtggc actgactgat acgccggagc cgaccagacc agttacaaat cccttctggc
      301 gtaacaagca tgatgagcgc cctgaggggg ctcccggaaa cgtcccacgg tgcgagctgc
      361 atcttaaatc aggtgtggta aaactgtttg accctctcac tccggaactg ttacgggcgc
      421 taatccgcga aatgaaaggg ggtatccgat gataacgctg ccgaccggta ccaaaatctg
      481 gatcatcgct ggcatcacag atatgcgttg tggcttcaat ggcctggctt cgaaggtgca
      541 gaacacgctg aaagatgacc cgttctccgg gcatatcttc gtcttccggg gccgcagtgg
      601 caaaatggtg aaaatactgt gggccga
//
LOCUS       DAJVQH010000342          616 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJVQH010000342 DAJVQH010000000
VERSION     DAJVQH010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.348"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>616
                     /locus_tag="OQM87_004345"
     CDS             <1..>616
                     /locus_tag="OQM87_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vimentin yjdA"
                     /protein_id="HCS1510193.1"
                     /translation="QFRPELEKIVSGIETGFRGTALYATENIAGRINTRLEDEGFTVK
                     ISFPAVSQLQTRLAVKTNLSALMEERTETVTRRRRQRGLWGKICGAFGTSDWGWETYK
                     EDVSRSVININTVRKEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREKVEQL
                     RNTLIQSSEDHKRDQQAQERLTRRLQALNERVPELITDSKALRE"
BASE COUNT          169 a          143 c          177 g          127 t
ORIGIN      
        1 cacagttccg gcctgagctg gagaaaatcg tcagcgggat tgaaacaggt tttcgtggca
       61 cggccctgta cgccacagaa aacattgccg gtcgcatcaa tacccgcctg gaggatgagg
      121 gctttaccgt aaaaatcagt tttccggcag tcagtcagtt acaaacccgg ctcgcggtaa
      181 aaacaaatct gagtgcgctt atggaggaaa gaacggagac cgtcacccgt cgccgtcggc
      241 agcgtggttt atggggaaag atttgtggag cgtttggcac cagtgactgg ggctgggaaa
      301 cctataaaga ggatgtgagc cgcagtgtta tcaatatcaa caccgtcagg aaggaagtta
      361 tgtcactgac ccgggcatat ttcggggagc tgcaggcatc cattgagcag gatattaacc
      421 agcccgtccg ccaggaaatc gatgcctttt tctgcgcatt cagggagaaa gttgaacaac
      481 tgcgtaacac gctgattcag agctctgaag atcataaacg cgatcagcag gcacaggaac
      541 ggcttaccag gcgacttcag gcattaaatg aaagggttcc tgagctcatt actgacagta
      601 aggcgctgag ggaaga
//
LOCUS       DAJVQH010000343          615 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJVQH010000343 DAJVQH010000000
VERSION     DAJVQH010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.349"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(14..>615)
                     /gene="ospZ"
                     /locus_tag="OQM87_004346"
     CDS             complement(14..>615)
                     /gene="ospZ"
                     /locus_tag="OQM87_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCS1510194.1"
                     /translation="HGYFNESPNIYDKKYISGITRSMAQLKIEEFINEKSRRLNYMKT
                     MYSPCPEDFQPISRDEASTPEGSWLTVISGKRPMGQFSVDSLYHPDLHALCELPEISC
                     KIFSKENSDFLYIVVVFRNDSPQGELRANRFIELYDIKREIMQVLRDESPELKSIKSE
                     IIIAREMGELFSYASEEIDSYIKQMNDRLSQIKARMPVT"
BASE COUNT          186 a          124 c           91 g          214 t
ORIGIN      
        1 caattaatat acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca
       61 tttgttttat gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac
      121 gagcaataat aatctcagat ttaatagact ttaactctgg cgactcatct cttaacactt
      181 gcataatctc tctttttata tcatatagtt ctataaaccg atttgcccgt agttctcctt
      241 ggggagaatc atttctgaat acaacaacta tatacaaaaa atcactattt tctttagaga
      301 agatcttaca actaatctct gggagctcac ataatgcatg taagtcagga tggtacaaac
      361 tatccacaga aaattgcccc ataggacgtt ttccagatat aactgtcaac cagcttcctt
      421 ccggtgtact ggcttcgtct ctggaaatag gctgaaaatc ttccggacat ggagaataca
      481 ttgtcttcat ataattaagt cgtctggatt tctcattaat aaactcctct atttttaatt
      541 gtgccatact tctggttata ccagagatat acttcttgtc atatatgttt ggtgattcat
      601 taaagtaacc atgtt
//
LOCUS       DAJVQH010000344          595 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJVQH010000344 DAJVQH010000000
VERSION     DAJVQH010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.350"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..309
                     /locus_tag="OQM87_004347"
     CDS             <1..309
                     /locus_tag="OQM87_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510195.1"
                     /translation="EAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKERTR
                     EMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWMDSL
                     "
     gene            393..>595
                     /locus_tag="OQM87_004348"
     CDS             393..>595
                     /locus_tag="OQM87_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510196.1"
                     /translation="MNMTKNAFQFGIEPVHITDNDNLQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVL"
BASE COUNT          164 a          158 c          142 g          131 t
ORIGIN      
        1 gaagctggcg tgaccattgg taacttcttc agtaatgacc tgatgaccgc ctaccaacgc
       61 cgccagatga acaaacgagc tgaagctgag cgtatcgcta aagagctagc gtctcagaaa
      121 gagcgtaccc gcgaaatgtt catgaccgaa gacgaatggc aaaaagagct acagcgccgc
      181 gaacaggtca aagctgaacg ccggacctat ggtgaaaacc tccgcagcgc tactcattcc
      241 gctggtcgct cacgtgccgc tatcgtcgct gaccttgagt caggcggtaa ctggatggac
      301 agcttgtaag caagcgtccc tcatctgaga acacacggtc cctgactgcc tgttagggac
      361 ttcaagtatt tgactcaagg agattaatta taatgaatat gactaaaaac gctttccagt
      421 ttggcattga gcctgttcat atcaccgaca acgacaacct tcaggttaat gagggccttc
      481 cgactaacgc cgaccctcag gtctatgccc tccagttagc taagactgtt aaggctatgc
      541 tgaacggtgt acttaaagac gctcaagata acatcccgtt ccctgtagaa gtcct
//
LOCUS       DAJVQH010000345          590 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJVQH010000345 DAJVQH010000000
VERSION     DAJVQH010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.351"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            23..>590
                     /locus_tag="OQM87_004349"
     CDS             23..>590
                     /locus_tag="OQM87_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCS1510197.1"
                     /translation="MFTANQCWTSFLDAGGLRDIEVEAVTKMLACGTRILGVKEYICD
                     KPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTLWPVF
                     ESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLN
                     KHGQWKKLSFLKDAMRSRWMWNMRQRLLK"
BASE COUNT          124 a          166 c          178 g          122 t
ORIGIN      
        1 tactccccgc ccgctgaaac gcctgttcac agccaaccag tgctggacgt ccttcctgga
       61 tgcgggcggt ctgcgcgata tcgaggttga ggctgtcacc aaaatgctgg cctgcggcac
      121 gcggatactg ggcgtgaagg aatacatctg cgataaacct gagtgccccc acgtcagata
      181 cgtcactaac tcatgcggca gccgtgcctg cccgtcctgc ggaaaaaagg ccacagacct
      241 gtggatagcg acacagctga atcgtcttcc tgactgcgac tgggtacacc tggtcttcac
      301 cctgccggac acgctgtggc cggtgttcga aagcaaccgg tggctgctga atgacgtgtg
      361 ccgtctggcg gtggagaatc tgctgtatgc cgcccggaaa cgggggcagg aacccggtat
      421 cttctgcgcc atccacacgt atggccgtcg tctcaactgg catccgcatg tacatgtgtc
      481 tgtaacctgt ggaggtctga ataagcatgg tcagtggaaa aagctgagct tcctgaaaga
      541 cgcgatgcgt tcgcggtgga tgtggaatat gcggcaacgg cttctgaaag
//
LOCUS       DAJVQH010000346          585 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJVQH010000346 DAJVQH010000000
VERSION     DAJVQH010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.352"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..453
                     /gene="ospC2"
                     /locus_tag="OQM87_004350"
     CDS             <1..453
                     /gene="ospC2"
                     /locus_tag="OQM87_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCS1510198.1"
                     /translation="TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSL
                     GNAKSDMALYLLSKFNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFIN
                     NGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          212 a           83 c          119 g          171 t
ORIGIN      
        1 acagataact tcaaaaagat aagactcaga gatatatctt tggaggatgc cattaaagca
       61 tccaattatg aagaaattaa caataaggtg actgataaaa aaatggctca tcaagctctt
      121 gcttattctc taggcaatgc aaaatctgat atggcacttt atttactatc taaatttaat
      181 tttacaaaac aagatgtcgc agagatggag aaaatgaaca acaatatgta ttgtgagctg
      241 tatgatgttg agtatttact tagtgaagat agtgccaact ataaagtgct agaatatttt
      301 attaataatg gattggttga tgtaaacaaa agatttcaaa aagcaaatag tggggacact
      361 atgcttgaca atgcaatgaa aagcaaagat tcaaaaacaa ttgatttttt attaaaaaat
      421 ggagcggtat caggcaaacg atttgggagg tgacctaaaa tatatttagg ttgtgccctg
      481 aatctgacgt tgtcattttt gttatattga tcgatatagg ggatggtgca gaccgttccc
      541 ctgcaacaac acattgcatg tggtgaacgc cccgggaatc ctgga
//
LOCUS       DAJVQH010000347          576 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJVQH010000347 DAJVQH010000000
VERSION     DAJVQH010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.353"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            90..155
                     /locus_tag="OQM87_004351"
                     /pseudo
     CDS             90..155
                     /locus_tag="OQM87_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            383..>576
                     /locus_tag="OQM87_004352"
     CDS             383..>576
                     /locus_tag="OQM87_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1510199.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFV"
BASE COUNT          184 a          120 c          131 g          141 t
ORIGIN      
        1 ctatagatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa
       61 actgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt
      121 tgctttgtga tattaatcct gtcacgttac gcgcgtgacg caacattttt aaaaatcgac
      181 gaagatcgtc caggagcgcc gtttttcaag ggttggatag atgattcgtt taccatgtcg
      241 acgaacttcg acgaatttcg ccgataaaaa gtgtcccata catgccccat tgatgcccca
      301 taaacaatat caggaattga cactggttat ccatacagat aaaaataaca cctgtacata
      361 actacagtta caggagggtg ttatgcgtgt tgagatctgt atcgctaaag aaaaaatcac
      421 taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac gcatcagtaa
      481 acgttatgac gatgtagagg tgatcgtaaa agccaccagc aacgatggcc tttctgttac
      541 acgcaccgca gataaggatt ctgcaaaaac atttgt
//
LOCUS       DAJVQH010000348          576 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJVQH010000348 DAJVQH010000000
VERSION     DAJVQH010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.354"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(260..>576)
                     /locus_tag="OQM87_004353"
     CDS             complement(260..>576)
                     /locus_tag="OQM87_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCS1510200.1"
                     /translation="KKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKD
                     GANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRF
                     EI"
BASE COUNT          167 a          115 c           97 g          197 t
ORIGIN      
        1 ggagtcaagt tcccgctccg acataactgc gtgcaatgga ttttgaagga accgtcagcc
       61 cattccccgg aggcaaacat gggctggcaa aagtgtagcg gtattaggca cagctattta
      121 ggaaagaggg agcatctcca tctcattaaa tggaccacgc cagacaattc actgtcatgc
      181 tgatgctgca ctgtggggac agaatcactc atgatgactt cgataatcga cgacattatt
      241 atttggccga gcttttaggt catatctcaa atcgtttgcc taatattgct ccatttttta
      301 ataaaaaatc aatcattttt gaatctttgc ttttcattgc attatcaagc atagtgtccc
      361 cactatttgc tttttgaaat tttttgttta catcaaccaa tccattattg ataaaatatt
      421 ctagcacttt atagttggca ccatctttac taagtaagta ttcaacatca tacagattac
      481 aatatctatt gttgttcatt ttctccatct ctgcgacatc ttgttttgta aaattaaatt
      541 tagatagtaa ataaagtgct atatcagctt ttttat
//
LOCUS       DAJVQH010000349          557 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJVQH010000349 DAJVQH010000000
VERSION     DAJVQH010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.355"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>557
                     /gene="ydfJ"
                     /locus_tag="OQM87_004354"
     CDS             <1..>557
                     /gene="ydfJ"
                     /locus_tag="OQM87_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCS1510201.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDK"
BASE COUNT          128 a          121 c          150 g          158 t
ORIGIN      
        1 catcttgctg gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg
       61 tgccggtacg atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt
      121 tgtggctatg ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt
      181 cttcattctc agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctggcgag
      241 cgttgtcgtg atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt
      301 tgagaaggtt aatgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt
      361 ccagagcaaa tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg
      421 tttaattcag actttccttg caggctattt agtacagacg ttattgttta acaaagcaat
      481 tccaacagat gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg
      541 ttggttatcc gataaaa
//
LOCUS       DAJVQH010000350          557 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJVQH010000350 DAJVQH010000000
VERSION     DAJVQH010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.356"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>557
                     /locus_tag="OQM87_004355"
     CDS             <1..>557
                     /locus_tag="OQM87_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1510202.1"
                     /translation="MHKHHEIHRVKPLMPALCRIRQGKKVINWETHSLTVDNNQIILF
                     PCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIRNTTGFCLPQNPELIY
                     CWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRCYNLMLSEP
                     RTKWTANKVARYLYISVSTLHRRLA"
BASE COUNT          179 a          105 c           97 g          176 t
ORIGIN      
        1 ggatgcataa acatcatgag attcacaggg ttaagcctct tatgccagct ttatgtcgta
       61 tccgtcaggg aaaaaaagtt atcaattggg agacgcatag tctaactgtt gataataatc
      121 agataatatt atttccctgt ggatatgaat tttatattgt gaattatcca gaagcaggtc
      181 tttatcttgc agaaatgctt tattatccca ttgatctaat tgaaaagttt caaaaatttt
      241 atgcgataac tgatcaaatt cgtaacacga caggtttctg cttacctcag aaccccgagt
      301 taatatattg ttgggagcaa ctaaaaacat ctatttcccg aggcttctca acgcaaattc
      361 aggaacactt agcaatgggc gttctacttt cattaggagc acatcatgtt aattgtttac
      421 ttttatcaga tagtaaacaa tcattaataa gtcgttgtta taacctaatg ctatccgaac
      481 ctagaacaaa atggacagca aacaaggtag cgagatatct ctacatttct gtttccacat
      541 tgcatcgccg tctggca
//
LOCUS       DAJVQH010000351          550 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJVQH010000351 DAJVQH010000000
VERSION     DAJVQH010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.357"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>550
                     /gene="yqhC"
                     /locus_tag="OQM87_004356"
     CDS             <1..>550
                     /gene="yqhC"
                     /locus_tag="OQM87_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCS1510203.1"
                     /translation="GEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARIL
                     GKQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEA
                     NMSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFS
                     REFKRYFGVTPGEDAARMRAMQG"
BASE COUNT          134 a          138 c          162 g          116 t
ORIGIN      
        1 ttggcgaaga tgagcatttc cagccgtcga tggcagccag cgggattaac tccgccacgt
       61 tatcagaaga gattttattc gcggcggagc ggttactcga cgtgatggag cgaccactgg
      121 atgcgcgtat tctcggcaaa cagatcatcc gcgaaattct gtactacgtg ctgaccggac
      181 cttgcggcgg cgcgttactg gcgctggtca gtcgccagac tcacttcagt ctgattagcc
      241 gcgtgctgaa acgaattgag aataaataca ccgaaaacct gagcgtcgag caactggcgg
      301 cagaagccaa catgagcgta tcggcgttcc accataattt taagtctgtc accagcacct
      361 cgccgttgca gtatttgaag aattaccgtc tgcataaggc gcggatgatg atcatccatg
      421 acggcatgaa ggccagcgca gcagcgatgc gcgtcggcta tgaaagcgca tcgcaattta
      481 gccgtgagtt taaacgttac ttcggtgtga cgccggggga agatgcggca agaatgcggg
      541 cgatgcaggg
//
LOCUS       DAJVQH010000352          535 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJVQH010000352 DAJVQH010000000
VERSION     DAJVQH010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.358"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>535
                     /locus_tag="OQM87_004357"
     CDS             <1..>535
                     /locus_tag="OQM87_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCS1510204.1"
                     /translation="EGFVHDLTSLTRDVATGVLARSMDVDIYNLHPAHAKRIEEIIAE
                     NKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHA
                     NPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQPSGAMTDEQATAETVEPNATEHH
                     QNTQPLDAQSQVNSVDEK"
BASE COUNT          155 a          144 c          128 g          108 t
ORIGIN      
        1 gaaggtttcg tccacgatct gacgtcactg acgcgcgatg tagccactgg cgtactggcc
       61 cgttcaatgg acgtggacat ctataacctt catccggcac acgctaaacg cattgaggaa
      121 attatcgctg aaaataaacc acccttttct gttttccgcg acaaattcat caccatgcct
      181 ggcgggctgg attattcccg cgccatcgtg gttgcgtccg tgaaagaagc accaattggg
      241 atcgaggtca tccccgcaca cgtcactgaa tatctgaaca aagtactgac tgaaaccgat
      301 catgccaacc ctgatccgga aatcgtggat attgcctgcg gtcgctcctc tgccccgatg
      361 ccgcagcgag taacagaaga aggaaaacag gacgatgaag aaaaaccaca accatctggc
      421 gcaatgacag atgaacaggc aacggctgaa acagtggaac cgaatgcaac tgaacatcat
      481 cagaacacgc agccgctgga tgctcagtca caggtaaatt ctgttgatga gaaat
//
LOCUS       DAJVQH010000353          532 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJVQH010000353 DAJVQH010000000
VERSION     DAJVQH010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.359"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>532
                     /locus_tag="OQM87_004358"
     CDS             <1..>532
                     /locus_tag="OQM87_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HCS1510205.1"
                     /translation="YLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVG
                     WAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMS
                     RRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGL
                     PPNESENRYWKNSNSVA"
BASE COUNT          136 a          129 c          161 g          106 t
ORIGIN      
        1 actaccttga aaggcagttc gccgtgaccg agccaaatca ggtgtggtgc ggtgatgtga
       61 cctatatctg gacgggtaag cgctgggcgt acctcgccgt tgttctcgac ctgttcgcaa
      121 gaaaaccagt gggctgggcc atgtcgttct cgccggacag caggctcacc atgaaagcgc
      181 tggaaatggc atgggaaacc cgtggtaagc ccggcggggt gatgttccac agcgatcagg
      241 gcagtcatta tacgagcagg cagttccggc agttattgtg gcgataccag atcagacaga
      301 gtatgagccg gcgcggaaac tgctgggata acagcccaat ggaacgcttc ttcaggagtc
      361 tgaagaacga atggatgccg gtggtgggtt acgtaagctt cagcgaggca gctcacgcca
      421 taacggacta tatcgttgga tattacagcg cactaagacc gcacgaatat aacggtgggt
      481 tacccccaaa cgaatcggaa aatcgatact ggaaaaactc taactcggtg gc
//
LOCUS       DAJVQH010000354          527 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJVQH010000354 DAJVQH010000000
VERSION     DAJVQH010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.360"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..417
                     /locus_tag="OQM87_004359"
     CDS             <1..417
                     /locus_tag="OQM87_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCS1510206.1"
                     /translation="RSGNMFNQLLASQKMTKTAVNEALRRLKKAGIDKPELEAFLREM
                     IEGKQKSWLTHCTDAEALKIDSVIGEVLADHPGLLNVLSQRYVGRGMSKRRMAELLNE
                     QYPEWALITCRRRVDQWLRVAEFILYSPMREAFDYA"
BASE COUNT          165 a          100 c          139 g          123 t
ORIGIN      
        1 aggtcaggga atatgtttaa ccagttgctg gcatcccaaa aaatgacaaa aacagccgtc
       61 aatgaagccc tgcgcagatt gaaaaaagcg ggaattgata aaccagagtt ggaagcattt
      121 ttgcgtgaga tgatcgaagg aaagcaaaaa agctggttaa ctcactgcac tgacgcagag
      181 gcactaaaaa ttgatagtgt tataggtgaa gttctggcgg atcatccagg actactaaat
      241 gtcctgagtc agcgttatgt ggggcgaggg atgagtaaga gaaggatggc cgagttatta
      301 aacgaacagt acccagagtg ggcgttgatt acatgccgac gtcgtgttga tcagtggttg
      361 cgtgttgctg agttcatttt gtattcaccg atgagagaag cgttcgatta tgcttaaaaa
      421 atcattgcaa aatgagccac aaactgcttc aattcaggta cagcttcgca aagctgtatc
      481 gcgaggcgaa ccaagcgcat gaactttacc agaacccgcc attgagc
//
LOCUS       DAJVQH010000355          519 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJVQH010000355 DAJVQH010000000
VERSION     DAJVQH010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.361"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..380)
                     /gene="ycaL"
                     /locus_tag="OQM87_004360"
     CDS             complement(<1..380)
                     /gene="ycaL"
                     /locus_tag="OQM87_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCS1510207.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDN"
BASE COUNT          136 a          124 c          104 g          155 t
ORIGIN      
        1 tcgttgtcat tcatcatgtc catcaggcca ctgtagacac gaacacagcc gttcgccatc
       61 gcccatgcgt tgacgtcgct ggtcatataa accttatagt tgaccggcgt gccattaata
      121 ttgttaccca atgctttggc gatttttgcc agacgtttac cgtacttgct ggatttactt
      181 gcgacttgat tgccgctgtc catttcggca cagccctgat tggcaatcgc ttttgcatcg
      241 gcatcgctta atgttgctgc tttataggca tttaaaccgg agctgatagc catattggta
      301 tcaataccgt gggtattttg acaccctgtc agtaatgctg cagaggtcgc aatcgccagc
      361 agtaatttag tattcttcat gtgaatcatc cgttgattaa tcatatataa atattatgta
      421 tcaacaactt acattgacaa gcgacgctac cattaatcaa aatgactcac aaggtccgaa
      481 aaaaaacgcc gacaatgtgc cgtaatgcca gtcagttaa
//
LOCUS       DAJVQH010000356          517 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJVQH010000356 DAJVQH010000000
VERSION     DAJVQH010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.362"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            163..510
                     /locus_tag="OQM87_004361"
     CDS             163..510
                     /locus_tag="OQM87_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1510208.1"
                     /translation="MEEEIFTREEAASYLKVDKGTITQWIRSGRLQAVKINPDKPKSP
                     YRICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLK
                     HRTKGKRKNFTIV"
BASE COUNT          172 a          115 c          127 g          103 t
ORIGIN      
        1 tctgaaatga agatacacgg aaacaaagca ccagagactt acagaagaat tagcgagtta
       61 cgcaaggact aaccacagcc tcatactcga tgaggcctgt tcattgctca atgatatcca
      121 gacctaccgc aataatacca atccaataaa tggagattcc aggtggaaga agaaatcttc
      181 actcgtgaag aggcagcgtc gtatctgaag gtagacaaag gcactatcac gcagtggata
      241 cgaagtggac gacttcaggc cgtaaagata aatccagata aacctaaaag cccatatcgc
      301 atttgcaagt cagactgcat tgcggcgctt aagtctgtga gacacaatag cgcggtgaat
      361 gcggttgatg tgcaggaggt taaagcatgt caatcaaact acgcggtggc acgtggcact
      421 gcgatttcgt cgcgccagat ggatcaagag ttagacgctc tcttgaaaca tcggacaaaa
      481 ggcaagcgca agaacttcac gatcgtctga aagcaga
//
LOCUS       DAJVQH010000357          513 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJVQH010000357 DAJVQH010000000
VERSION     DAJVQH010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.363"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..502
                     /locus_tag="OQM87_004362"
     CDS             <1..502
                     /locus_tag="OQM87_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1510209.1"
                     /translation="PTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQS
                     LYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVG
                     RKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPW
                     NFSSVK"
BASE COUNT          149 a          109 c          153 g          102 t
ORIGIN      
        1 tccgacagaa atgactcagg aagcgctcag acggatagca gagttatacg acatagaagc
       61 ggagatacgt ggcagtccgg cagaggaacg gcttgcagtc agaaaagcca gaagcgtcca
      121 gttgatgcag tcgttgtacg actggataca gttgcagagg aaaacgctgt cgaaacatgc
      181 ggagatggcg aaggcgttcg actatatcct gaatcactgg aatgcgctga acgagttctg
      241 tcgtgacggc tgggtggaaa tagacaacaa catcggtgaa aacgcgttac gatcggtggc
      301 ggttggaaga aaaaattatc tctttttcgg ctcagacaag ggaggagaaa gtgcggcgat
      361 catctacagt ctgctggtca cctgcaaaca gaacgaagtg gagccggagg actggttgcg
      421 cgaagtgatc gagaagctca atgactggcc gtcgaaccaa gtgcatgaac tgctgccctg
      481 gaacttctcg tctgtaaaat aatccttacg cta
//
LOCUS       DAJVQH010000358          512 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJVQH010000358 DAJVQH010000000
VERSION     DAJVQH010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.364"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>512
                     /locus_tag="OQM87_004363"
     CDS             <1..>512
                     /locus_tag="OQM87_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1510210.1"
                     /translation="YLEPETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQ
                     NWKPATLSENRASHSTLIEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSM
                     PLSDLLNRESKTDCLDVVTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKA
                     SDARDAFSPE"
BASE COUNT          133 a          138 c          127 g          114 t
ORIGIN      
        1 taccttgagc ctgagactgg cagtctaaga ccgtccgtct ccctatctgt attggcggaa
       61 cctgaagtcc ctgaacctaa agcactgacc ctcgcgtctc tcattgagca gtacgagcag
      121 gagaacgccc agaactggaa gccagcgacc ctaagcgaaa accgagcgtc acactctacg
      181 ctaatcgaga tattcgacta tctggatatt caggatgtcg gcaaggcgac ccgtgcagat
      241 atgctcagag ttcgtgaagt cctccagcag ctaccgaaga accgcaagca acgctttaag
      301 tctatgccgc tgtctgactt gctgaaccgg gagtctaaga ctgactgtct ggacgtcgtt
      361 accattaaca ataaatatct tatcaagatg gctgctgtgt ttaagtgggc ggtacgcaac
      421 gacttgatag ctaagaacct gaccgaaggt cttgagctga aagtccctca gcgtaaagcc
      481 tccgacgctc gtgatgcgtt cagtcctgag ca
//
LOCUS       DAJVQH010000359          511 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJVQH010000359 DAJVQH010000000
VERSION     DAJVQH010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.365"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            90..511
                     /locus_tag="OQM87_004364"
                     /pseudo
     CDS             90..511
                     /locus_tag="OQM87_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
BASE COUNT          141 a          139 c          156 g           75 t
ORIGIN      
        1 atgaggcgac taaacaggcg tcagcagcga agggcagtgc cacgacggca tcctctaagg
       61 cgacagagtc tgcaggcagt gcgacggcgg cagcgcagag caaaagcgcg gcagaatccg
      121 cggcaatgcg cgctgagaca gcagcaaaac gggcagagga tattgctgat gtcgtatctc
      181 tggaagatgc gagcacgacg aaaaagggga tagtgcagct cagcagtgcg accaacagtg
      241 catccgagtc actggccgcg acagcgaaag cggttaaggt ggtaatggat gaaacaaaca
      301 agaaagcgcc cctgaacagt ccggctctga ccggaacgcc aaccacaccc actgcgccaa
      361 aggggactaa taatactcag atcgccagta cggcttatgt tatggccgcg attgctgccc
      421 tcgtggactc gtcgcctgat gcactgaaca cgctgaatga actggcggcg gcgctgggca
      481 acgacccgaa ttttgcgacc accatgacta a
//
LOCUS       DAJVQH010000360          510 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJVQH010000360 DAJVQH010000000
VERSION     DAJVQH010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.366"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>510
                     /locus_tag="OQM87_004365"
     CDS             <1..>510
                     /locus_tag="OQM87_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1510211.1"
                     /translation="PESLSHITTESQRRSLVLKAGGKYWHVYMSKKTAGGRNTARYLG
                     RYLKKPPIAASRLAHYNGGASLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFK
                     MVRYFGFLASRVCGEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGR
                     MVYRRAIAGL"
BASE COUNT          123 a          122 c          173 g           92 t
ORIGIN      
        1 gccggagtcg ttgtcacata tcacgacgga atcacagagg agaagcctgg tgctgaaagc
       61 cggaggaaaa tactggcatg tgtacatgtc gaaaaaaacg gccggcggga ggaatacggc
      121 gcgctacctg ggtcgttatc tgaagaagcc gccgatagcg gcctcccggc tggcacatta
      181 caacggaggg gcgagcctga gcttccgtta cctggaccac aaaacgggag aaacggcgac
      241 ggaaacgctg acacagcgtg agctggtcgc gaggctgaaa cagcacatcc cggagaagtt
      301 ttttaagatg gtgaggtact tcgggttcct tgccagccgg gtgtgtggag agaagctgcc
      361 gcaggtgtac cgtgctctgg ggatggataa accggaacca gtggcgaaag tgtgctatgc
      421 acaaatggtg aaacagttcc tgagtcgtga cccgttcgaa tgcgtgctgt gtggcggccg
      481 gatggtatac cgccgggcca tcgcgggact
//
LOCUS       DAJVQH010000361          509 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJVQH010000361 DAJVQH010000000
VERSION     DAJVQH010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.367"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..248)
                     /locus_tag="OQM87_004366"
     CDS             complement(<1..248)
                     /locus_tag="OQM87_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCS1510212.1"
                     /translation="MHEKNITLLCDEADRLLQLSINLLRQMVDEPDVLSDGKNENGQL
                     FDKQKALKRIKELEGEQIKTARREMVLAVVGTMKAGKST"
BASE COUNT          134 a          129 c          105 g          141 t
ORIGIN      
        1 gttgatttgc ctgctttcat cgtgccgaca acagccagca ccatctccct gcgggcggtt
       61 ttgatttgtt cgccctccag ctccttaatt cttttcagtg ctttctgttt atcaaaaagc
      121 tgcccgtttt cgttcttacc gtcagataac acatccggct catcaaccat ttgccggagc
      181 agattgatgc tcagttgcaa aagccggtcg gcttcatcac aaagcagggt gatgttcttt
      241 tcgtgcatta tttaaccttc ctgagacgca ttttcatgac aaccacggag gaggccattt
      301 actacaagcg atcgcttata acgatcaata taatagaaat gatcgtcata ttctattaat
      361 atgaaaaaca caacgcaagg gggacaaccc gggtatggct gcgtttgcat tgtgttttca
      421 tagagttaaa aatctgtaac gacaacccgg cacatcacta ccgggttaag aagaaaatcc
      481 agttccatac cgcgcgggcc acagagacc
//
LOCUS       DAJVQH010000362          506 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJVQH010000362 DAJVQH010000000
VERSION     DAJVQH010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.368"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..258
                     /locus_tag="OQM87_004367"
     CDS             <1..258
                     /locus_tag="OQM87_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510213.1"
                     /translation="YNGEDRGVFMMAKRFTALSFGCFRHDGESITVVLSRDGDSQSHD
                     LEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
BASE COUNT          126 a          114 c          130 g          136 t
ORIGIN      
        1 tacaacggtg aagaccgtgg cgtgttcatg atggctaagc gttttacggc tctgagcttc
       61 ggttgcttcc gtcacgatgg cgagagcatt actgtggtcc tttcacgtga tggagactcc
      121 cagtctcacg accttgagat tctcggcaag gtgtttgttg tagctgaagc tgctacgacc
      181 atcaagatgg gtactggctc ggctactgct gtcctgcctg tagtcaaacg cctgagcttc
      241 actaaagaag ctaactaaca ataacgacta ttggtcagac ttccgaactg gatgtctggc
      301 ctttattttg tactagtaat ctagttcgtg aatacagaaa gtaggagggc caaatggacc
      361 tacacaaagt ctatctcgtg gtcttcctgc taaagctggg actggtggtc tggctatggg
      421 ctattagacg atgagttttg actgaaaaat gtgagacctc atctaatata aagagcgagc
      481 caaaagcccc catagcctga cctgag
//
LOCUS       DAJVQH010000363          506 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJVQH010000363 DAJVQH010000000
VERSION     DAJVQH010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.369"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            30..491
                     /locus_tag="OQM87_004368"
     CDS             30..491
                     /locus_tag="OQM87_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCS1510214.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
BASE COUNT          161 a          102 c          111 g          132 t
ORIGIN      
        1 tttcttgatt aaataaaatg gagttttaca tgaaggcttt caataagctg ttttccctcg
       61 ttgttgcatc tgttctggtt ttctctcttg ctggctgcgg tgacaaagaa gaatcgaaga
      121 aattcagcgc caatctgaac ggcactgaaa ttgccattac ctatgtctac aaaggtgaca
      181 aggtgcttaa gcaatcttct gaaaccaaaa ttcaatttgc ctccattggt gcaaccacca
      241 aagaagatgc tgccaagaca cttgagccgt taagcgccaa atacaaaaac atcgcgggtg
      301 ttgaagaaaa atcaacctat accgatacct acgcgcagga aaacgtgact atcgatatgg
      361 aaaaagtgga ttttaaagcc ctgcagggta tttcaggaat caacgtttct gctgaagatg
      421 ccaaaaaagg tatcactatg gcgcaaatgg aactggtgat gaaagccgct ggttttaaag
      481 aagtgaaata atctgtcggc ggccat
//
LOCUS       DAJVQH010000364          505 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJVQH010000364 DAJVQH010000000
VERSION     DAJVQH010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.370"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(3..218)
                     /locus_tag="OQM87_004369"
     CDS             complement(3..218)
                     /locus_tag="OQM87_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS1510215.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          161 a          125 c          102 g          117 t
ORIGIN      
        1 cattattcac ctctcgcagc cttacgcttg tcttctctga ttttgaagta cagatttgtc
       61 agataagtca ggaagcccag aaccagactc cccagtacac caatcgcagc ccactgtgac
      121 ggactgacct gatcaagcca ctgtaaaaac cagtagccag cactgcctgc ggaggtgccg
      181 taggcaatgc ccgttgaaat tttgtccatg gatttcatag cctcacctcc gcaaataacg
      241 gatggcgtag ttttacactg agaaatgaaa gggatttgaa aagaaaaaac cgcaaaagcg
      301 ggcgaaacga tatatacagt aaggaaagca ctctatccaa caaaccactc acagttaatc
      361 ggaataaaag cagagtgctt atgaatgatc gcctgcccga aggttagtat ttctgcacag
      421 caattttgca aaaaaaagcg atcattcata acttaaacgt ctttcagtca ctccgggatt
      481 tcccatcatc gcagactgaa agact
//
LOCUS       DAJVQH010000365          504 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJVQH010000365 DAJVQH010000000
VERSION     DAJVQH010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.371"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..>504)
                     /locus_tag="OQM87_004370"
     CDS             complement(<1..>504)
                     /locus_tag="OQM87_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS1510216.1"
                     /translation="LSLLLPDGNLLATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSK
                     EVLFHLALYRNNPRCKAVVHLHSTWSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLV
                     PYYRPGDKRIAQDLAELAADNQAFLLANHGPVVCGESLQEAANNMEELEETAKLIFIL
                     GDRPIRY"
BASE COUNT          114 a          143 c          132 g          115 t
ORIGIN      
        1 gataacggat cgggcggtca ccgagaataa aaatcagctt tgccgtttcc tccagctctt
       61 ccatattgtt ggcggcttct tgcaggcttt caccgcaaac cactgggcca tgatttgcca
      121 gtaaaaaagc ctgattgtct gctgccagtt ccgccagatc ctgtgcgatg cgtttatcgc
      181 ccggtcggta atagggcacc agcgggacat ttcccatccg catcaccacg tatggtgtga
      241 acggacgaat aacgttgctg ctgtccagcc cttgcaggca ggaaagcgcc gtcgaccatg
      301 tgctgtgcaa atgcaccacc gctttacagc gcggattgtt gcgatacagc gccagatgaa
      361 agagcacctc tttcgagggt ttgtcaccac ttaaccattc gccatccgcg gcgactttgg
      421 aaagccgctg cggatcgaga ttgcccaggc atgaacctgt cggtgtcgcc agtaaattcc
      481 cgtcaggtaa aagcagcgac agat
//
LOCUS       DAJVQH010000366          501 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJVQH010000366 DAJVQH010000000
VERSION     DAJVQH010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.372"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..373)
                     /locus_tag="OQM87_004371"
     CDS             complement(<1..373)
                     /locus_tag="OQM87_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1510217.1"
                     /translation="MWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTP
                     PEDSYYTPVVHGIAQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVR
                     ESNGEEQHFSVPFQTPAIALHE"
BASE COUNT          128 a          144 c          105 g          124 t
ORIGIN      
        1 cttcatgcag agcgatggcc ggcgtttgaa atggcacgga aaaatgctgc tcctcgccat
       61 tactctctct cactgtaaca tcaagctccc cgccaacggc cagcgttggt aaattatcca
      121 gcgcaaaagg ccctggcggc acagaacggg taaaaataat ttgcccattt tgtttaataa
      181 taacctgcgc ctcactttgt gctattccat gcaccacagg tgtgtagtag ctgtcctccg
      241 gcggagtcat cgcatcgtcg gtcgctaaca tcccgccgcg aaaaggtatg ctgtcaaaca
      301 cctcagacga cgtataagat tctccaagcg ttaatcggct tttaatcgca tttatgcctc
      361 gctcaaacca catgtaaccc ctttgccatc tttctgactg gccaagttcc ttgctccatg
      421 tcactgaact acgaaacctc caggcaccaa gattaaaccc tggagtgaac ctgccataac
      481 gagaattacg cgtggtagat t
//
LOCUS       DAJVQH010000367          498 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJVQH010000367 DAJVQH010000000
VERSION     DAJVQH010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.373"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..477
                     /gene="ybcL"
                     /locus_tag="OQM87_004372"
     CDS             <1..477
                     /gene="ybcL"
                     /locus_tag="OQM87_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCS1510218.1"
                     /translation="SNEIKTGEQLTTSHVFSGFGCEGGNTSPSLTWSGAPEGTKSFAV
                     TVYDPDAPTGSGWWHWTVANIPATVTYLPTDAGRRDGTKLPTGAVQGRNDFGYAGFGG
                     ACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGALVGYMLNANKIATAEITPVYEIK"
BASE COUNT          148 a           94 c          120 g          136 t
ORIGIN      
        1 agtaatgaaa taaaaacagg agagcaactt acaacgtctc atgtattttc tggatttggg
       61 tgtgaaggtg gtaatacatc gccctcatta acctggtctg gtgctcctga aggcaccaaa
      121 agctttgccg taactgtgta tgatccagat gctcctacag gcagtggatg gtggcactgg
      181 actgttgcta atattccagc aactgtaaca tatttgccca ctgatgcagg aagacgtgat
      241 ggtacaaaac ttccgactgg tgctgttcaa ggccgaaatg attttggtta tgcaggattt
      301 ggtggagcat gtcctcccaa aggagataaa ccccatcatt accagtttaa agtatgggct
      361 ctaaaaactg ataagattcc tgtggattct aattccagcg gagcgttagt tggttatatg
      421 cttaatgcca ataaaattgc aaccgctgag ataacaccag tttatgagat aaagtagggt
      481 aagagtatgc tggcaaaa
//
LOCUS       DAJVQH010000368          486 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJVQH010000368 DAJVQH010000000
VERSION     DAJVQH010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.374"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
BASE COUNT          126 a          101 c           93 g          166 t
ORIGIN      
        1 gtttttatct gtggttattt agataacatt atctaactgg acactttctt gtatgtatta
       61 tagctaaatg aacagatttt aatatgttgt atggttttca ctctgaaatc ccctgaattt
      121 aatcacgttt cctttactga aggcaatttt aaccgtagta tcaattatta acccttgggc
      181 ttgatatccc tctcctcttc tgggcacggg gaatgtcaga ttacttatta cagaaaccaa
      241 aagaattgta agcgtcatct cagtgtcgtc tatttcaatg tctgatgatt ggcgatgata
      301 gagtcctctt ataacgttaa tggactctat caatgtcaga ttctcttcgc ccccgcagga
      361 ttcgtgcctc ttactctatg actctatgga gtttaagctt gatcttattg aaaaatctta
      421 ccagctaggg gcctgcgtgg cccaactggc cagagagtac gggattaatg taagcgcccc
      481 atcagc
//
LOCUS       DAJVQH010000369          481 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJVQH010000369 DAJVQH010000000
VERSION     DAJVQH010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.375"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..>481)
                     /locus_tag="OQM87_004373"
     CDS             complement(<1..>481)
                     /locus_tag="OQM87_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCS1510219.1"
                     /translation="QQAFARQSQLLANLRARVNGFMAIEVPATQVSVSDAVSTYLFNS
                     QLLSRDDGSMMLVLPQECREHAGVWGYLNELLAADNPISELKVFDLRESMANGGGPAC
                     LRLRVVLTEEERRAVNPAVMMNDTLFNALNDWVDRYYRDRLTAADLADPQLLREGREA
                     "
BASE COUNT          111 a          152 c          118 g          100 t
ORIGIN      
        1 gcttcccgcc cttcgcgcag caattgcggg tcggccagat cggcagcagt aagacgatcg
       61 cggtagtaac gatccaccca gtcattgagc gcattaaaca aggtatcgtt catcatcacc
      121 gccggattca ccgcccggcg ttcttcttct gtcaatacca cccgcaaccg caggcacgcc
      181 gggccgccgc cattcgccat gctttcacgt aagtcaaaga cttttagttc gctaatcggg
      241 ttgtcagcgg caaggagttc attgagataa ccccatactc cggcgtgttc ccgacactcc
      301 tgaggcagca ccaacatcat ggaaccatca tcgcggctca acagttggct gttaaacaga
      361 taggtagaca ccgcatccga cacggaaacc tgagttgccg gaacttctat cgccataaaa
      421 ccgttcaccc gcgcccgcag gtttgccagt aactgtgact ggcgagcgaa cgcctgttgg
      481 t
//
LOCUS       DAJVQH010000370          477 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJVQH010000370 DAJVQH010000000
VERSION     DAJVQH010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.376"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..383
                     /locus_tag="OQM87_004374"
     CDS             <1..383
                     /locus_tag="OQM87_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkA"
                     /protein_id="HCS1510220.1"
                     /translation="RAYVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRG
                     VAEAIEAVAHGDESTSRVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHV
                     IMFLTDKKFITDVERLFQPSPFFL"
BASE COUNT          127 a          102 c          112 g          136 t
ORIGIN      
        1 gtcgcgctta tgtggatctg gttcagggga gcgtgatcga tattgcaatt tcaccacaac
       61 aagcaactat ttctgcgttg cttagccatg tgcgaaaagc agatattgtt ggtgtttcct
      121 cattgcgccg cggcgtagca gaagctattg aagccgttgc tcacggtgat gaaagcacct
      181 cacgcgttgt cggcagagtc attgacgaaa tcaagctacc gccaggaacg attattggag
      241 cggtggtacg tggaaacgac gtgatgattg ccaatgacaa tctgcgcatt gagcaaggcg
      301 atcacgtaat tatgttcctc acagataaaa agtttattac cgacgtcgaa agactcttcc
      361 agccaagccc tttcttcttg taattaataa ggcgtctaat gacgccttat tatttccctt
      421 tgattatcaa ggattaatta aattcattcc tggcaggaaa atggcttaac atttgtt
//
LOCUS       DAJVQH010000371          469 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJVQH010000371 DAJVQH010000000
VERSION     DAJVQH010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.377"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>469
                     /locus_tag="OQM87_004375"
     CDS             <1..>469
                     /locus_tag="OQM87_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1510221.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPSVGPITASLLSSQLGDGKQFSCSRDFAASTGL"
BASE COUNT          130 a          114 c          113 g          112 t
ORIGIN      
        1 ttgatgcaga agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
       61 gaactgagac ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag
      121 ataaggtgaa aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc
      181 cgacagggga tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg
      241 aatatctgag ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa
      301 tagcagagct ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca
      361 ttatgacaat tcctagtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg
      421 acgggaaaca gttttcatgc agcagagatt ttgcggcttc cacagggct
//
LOCUS       DAJVQH010000372          465 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJVQH010000372 DAJVQH010000000
VERSION     DAJVQH010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.378"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            35..465
                     /locus_tag="OQM87_004376"
                     /pseudo
     CDS             35..465
                     /locus_tag="OQM87_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a          111 c          132 g          100 t
ORIGIN      
        1 aatactagtt tttagactag tcattggaga acagatgatt gatgtcttag ggccggagaa
       61 acgcagacgg cgtaccacac aggaaaagat cgcaattgtt cagcagagct ttgaaccggg
      121 gatgacggtc tccctcgttg cccggcaaca tggtgtagca gccagccagt tatttctctg
      181 gcgtaagcaa taccaggaag gaagtcttac tgctgtggcc gccggagaac aggttgttcc
      241 tgcctctgaa cttgctgccg ccatgaagca gattaaagaa ctccagcgcc tgctcggcaa
      301 gaaaacgatg gaaaatgaac tcctcaaaga agccgttgaa tatggacggg caaaaaagtg
      361 gatagcgcac gcgcccttat tgcccgggga tggggagtaa gcttagtcag ccgttgtctc
      421 cgggtgtcgc gtgcgcagtt gcacgtcatt ctcagacgaa ccgat
//
LOCUS       DAJVQH010000373          464 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJVQH010000373 DAJVQH010000000
VERSION     DAJVQH010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.379"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>464
                     /locus_tag="OQM87_004377"
     CDS             <1..>464
                     /locus_tag="OQM87_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1510222.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWI"
BASE COUNT          126 a          106 c          105 g          127 t
ORIGIN      
        1 gtcattgctg atttagccgg tgaaagtgaa gatccacgtt ctgtttgtga acattattat
       61 tctttaatct cacaatatcg ggaaattcac tgggttttca tggtgtcacg ctcctggtat
      121 tcccaggctg tagaactgct catgtgccct acggcgacgt tattgtctga tgttgaaccc
      181 attgagaatc tggtcaagac cgtacgttcc ggcaatacgc atgcagagcg tatcagcgcc
      241 atgctgacct ctccggcaat gactgaaact catgatttta gctatcgctc cgtcattctc
      301 actctttcag agcgcaaggt actgcggcta ttaggtaaag gatggggcat caaccagata
      361 gcttcattgc ttaagaaaag taataaaact atcagcgccc aaaaaaacag tgcgatgcgt
      421 cgactggcaa ttcacagcaa cgctgaaatg tatgcatgga taaa
//
LOCUS       DAJVQH010000374          458 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJVQH010000374 DAJVQH010000000
VERSION     DAJVQH010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.380"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..436
                     /locus_tag="OQM87_004378"
     CDS             <1..436
                     /locus_tag="OQM87_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510223.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVKNYVAAVN"
BASE COUNT          127 a          104 c          119 g          108 t
ORIGIN      
        1 aagtcctgac acaaacgccg ccactgtaaa tcagacactt aaatgcgact caaaagcccg
       61 ggctgaccag cctggtgcat gtccaacaac gtacgagtta tacgaaggtg acgctaccta
      121 cagagctgct atcgacaaag cattaaaccc tgttgggtta aacggaatgt ttggggaaga
      181 cggctatatg gatggtcctg atggtggtgc gtatcctgta aacattaacg gaacgacctg
      241 ggtagaaggt ggcggttgta aagcccacgc ctgcggctgg gactatatcg taacactcta
      301 taacccaaaa acccatgaag tcgttggcta ccgctacttt ggtttagatg acccggccta
      361 cctggtttgg ttcggcgaaa ttggcgtgca tgaattcgcg tatctggtga aaaactacgt
      421 agctgcggtt aactaattta aggagtctgt catgaaaa
//
LOCUS       DAJVQH010000375          453 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJVQH010000375 DAJVQH010000000
VERSION     DAJVQH010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.381"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(206..>453)
                     /locus_tag="OQM87_004379"
     CDS             complement(206..>453)
                     /locus_tag="OQM87_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCS1510224.1"
                     /translation="RKRLKYAGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRK
                     PLAARQALSRSETVLQKSLEITEEADLALRSAMARIK"
BASE COUNT          113 a          127 c           89 g          124 t
ORIGIN      
        1 tcaaaaacac catcatacac taaatcagta agttggcagc atcacctaag tgcttttacg
       61 atgaatttca tgataactct ccttatgaca ttaataaatg cgttaatctt aaatctcacc
      121 caggtaatat atatctgaaa atattttcaa caacaccaga caggaaaaac accaccacag
      181 gcggtggcgt ttctttgggg cgtctttatt taatccgggc catcgcgctt ctcagtgcca
      241 aatctgcctc ttccgttatc tccagacttt tctgcaacac cgtttctgac cgggacagtg
      301 cctgacgtgc tgccagcggt ttccggatat gctccatcgt cagcatcgtg tcctcgtagc
      361 cctgctgacg ccaggcgtcg gtatgtgccg acgtgaaact gagcagaccg ccactgttgc
      421 caccatcacc tgcatatttc agccttttcc ggg
//
LOCUS       DAJVQH010000376          451 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJVQH010000376 DAJVQH010000000
VERSION     DAJVQH010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.382"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            281..>451
                     /locus_tag="OQM87_004380"
     CDS             281..>451
                     /locus_tag="OQM87_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1510225.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLP"
BASE COUNT          147 a           59 c           86 g          159 t
ORIGIN      
        1 aaaatgccaa atataaattt tgtgtatctt tttctgaaaa taaactaaag aaagggcttt
       61 atatatcacg catatttatt tgttggatag tcattagatg ttgaatcaat gaattactcg
      121 ttcagttaat atttaaatat tcatgaaatc tataaattaa agatttgtca cttattggat
      181 ttagtatgct tgatttaaat ggcggtcaat tattgttatt attaagtcta atgtcattta
      241 aaggcattat catatttacg aagagatcag gatgatgacg atgaaaaaaa gtgtattgac
      301 ggcgtttata actgtggtat gtgcaacgtc cagcgttatg gctgctgatg ataatgctat
      361 cacggatggc tcagtaacat ttaatggtaa agttattgct ccagcttgta ccctggtagc
      421 tgcgacgaaa gattcagtag tcacattgcc a
//
LOCUS       DAJVQH010000377          451 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJVQH010000377 DAJVQH010000000
VERSION     DAJVQH010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.383"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
BASE COUNT          128 a           90 c           84 g          149 t
ORIGIN      
        1 cagcagttta tcatttccca taatgtcact taccctcatc ggtaaaaaaa ttgcctttcg
       61 accattacga tacattattg attttggggt ttcagggaag taatccattt cgactataac
      121 tgacaggtca tcacgacgta tgactgcgta tttgctacta aatagtttct ttattttctc
      181 cacgatgcct ccaggtttat aagtacaaac ggttatatcc acatagagac aaaaatatta
      241 atctgaaaaa tatttatttc acgccgtata tttgattgtt taatgtgcaa gtacaatgac
      301 ttttattttt tgttgtgtat ataatcaaat atatggttat ttttcaccct gcgtattcag
      361 cacgcaacaa aaaacccgcc gaagcgggtt tagtgcgggt gcgttgagga tgcctgacac
      421 atgagaggtg gcgagggatt tctcccccgc c
//
LOCUS       DAJVQH010000378          444 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJVQH010000378 DAJVQH010000000
VERSION     DAJVQH010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.384"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..444
                     /locus_tag="OQM87_004381"
                     /pseudo
     CDS             1..444
                     /locus_tag="OQM87_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          102 a          125 c          130 g           87 t
ORIGIN      
        1 gttgctgccg cagcccgtga actcagcctg tatgaatctc agctctatgc gtggcgcagc
       61 aaactgcagc aacagatgac ctcgtctgag cgtgaaagtg aactggccgc tgaaaatgcc
      121 cgcctgaagc ggcaactggc ggagcaggca gaggaactgg ctatcctcca aaaggccgcg
      181 acatactttg cgaagcgcct gaaatgaagt atgtctttat tgaaaaacat caggctgagt
      241 tcagcatcaa ggcaatgtgc cgcgtgctcc gggtggcccg cagcggctgg tatacgtggt
      301 gtcagcggcg gacaggaata agccctcgtc agcagttccg tcagcactgc gacagcgttg
      361 tcctcgctgc ggcctttacc cggtcaaaac agcgttacgg tgcaccacgc ctgacggatg
      421 aactgcgtgc tcagggttac cact
//
LOCUS       DAJVQH010000379          441 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJVQH010000379 DAJVQH010000000
VERSION     DAJVQH010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.385"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..285)
                     /locus_tag="OQM87_004382"
     CDS             complement(<1..285)
                     /locus_tag="OQM87_004382"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510226.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDARNKKLVFWFVRVDDEGYPEIARCMEREFVTIP"
BASE COUNT          122 a          101 c           97 g          121 t
ORIGIN      
        1 aggaattgtc acaaattccc gctccatgca gcgggcaatt tccggatacc cttcgtcatc
       61 aacacgtaca aaccagaaga ccagtttttt gtttctggca tccacaaaga acggaatatt
      121 caggtctgcg caacactcaa cggcatcgtc aaaattatca aagcgcagaa cctcagcttc
      181 ttcttcgtca aaaaaatcat cttcgtgaag ttttacgaca tagcggagga gtttgcttct
      241 ttgaggtgca ggttttcgtt tgcgtggttt agctgaacgg gccatataac tacctgaaag
      301 acaatgatat tgtctgtttt tataacggta atcacaggcc atgacaagcc gcagccgtca
      361 ggccgcctac tcggggcggt ttagaacgct tatttagtac cagatcttac ttataataaa
      421 ggggcttggc agtttggata a
//
LOCUS       DAJVQH010000380          436 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJVQH010000380 DAJVQH010000000
VERSION     DAJVQH010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.386"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>436
                     /locus_tag="OQM87_004383"
     CDS             <1..>436
                     /locus_tag="OQM87_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCS1510227.1"
                     /translation="PLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKT
                     AQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQ
                     MELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY"
BASE COUNT           99 a          139 c          116 g           82 t
ORIGIN      
        1 cctcttcacc agattgtgaa tcgcctggac atcatgctgc cgggcaatcg ccccttcgtt
       61 gcccccagtg ccgttattca ggcccgccag cgcctgggaa gtgaggctgt ccgccgcgtg
      121 ttcacgaaaa cagcgcagct ctggcataac gccacgccgc atccgcactg gtgcggcctg
      181 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
      241 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      301 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      361 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      421 atggataaag gttatt
//
LOCUS       DAJVQH010000381          433 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJVQH010000381 DAJVQH010000000
VERSION     DAJVQH010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.387"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..212)
                     /locus_tag="OQM87_004384"
     CDS             complement(<1..212)
                     /locus_tag="OQM87_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1510228.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNIT"
     gene            complement(225..433)
                     /locus_tag="OQM87_004385"
                     /pseudo
     CDS             complement(225..433)
                     /locus_tag="OQM87_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
BASE COUNT          111 a           97 c          108 g          117 t
ORIGIN      
        1 gtaatattgt gccagccaga tgcagaacca ggttgttgca gattgcgcgt ataaagatcc
       61 ccgagattaa cgttagcttc tttggtttga atagtgcagg gtttagcgac tacccgacca
      121 ttaacagtga tagtgacatc ggcggcctgg agagttgctg tgaataacgc gcaaaacgta
      181 gctaacagga acctcttgtg aagtcttttc atatctgact ctgattattg aaattcaagg
      241 gtaaaggttg ccgaagccct caccagtccc ggattgacgg acttctgagt tgacttcaga
      301 cgagcggagt aaggcaaaat attgttctgt tctggtacca gtgggatcca ctgcatcccg
      361 gcatgaaggt cattcagttt caccggacgc atatttccgt ccagtatttc ttacccaaac
      421 cggaggccgt att
//
LOCUS       DAJVQH010000382          427 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJVQH010000382 DAJVQH010000000
VERSION     DAJVQH010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.388"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>427
                     /locus_tag="OQM87_004386"
     CDS             <1..>427
                     /locus_tag="OQM87_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510229.1"
                     /translation="DWAWLKQEWRRFTDRFVNSQAHIIMCGRAGYEYDFFEGDDGKRQ
                     LAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKDRSTRIDGQVFSNPT
                     FKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWL"
BASE COUNT          133 a           96 c          102 g           96 t
ORIGIN      
        1 cagactgggc gtggctcaaa caggagtggc gacgctttac cgatcgattt gttaacagtc
       61 aggcgcacat tattatgtgc gggcgtgcag gatatgagta tgactttttc gaaggtgatg
      121 atggcaaacg ccagttggca aaaaccggta taaaaatgaa agcagaaaca gaaaccggtt
      181 atgaaccctc catcctgatc cagatggaga agcaaatgaa catagaaacc ggtcaggtgt
      241 ggcgcaccgc cagaatacta aaagatcgct ccacccgtat tgatggacag gtattttcaa
      301 acccaacatt taaaaacttt cttcctcata tcgagtttct taacctggga ggtgaacacc
      361 tcggcatcga tacctcacgg gataacagtg aattatttgc caacgacggt acaccgacat
      421 ggctaaa
//
LOCUS       DAJVQH010000383          421 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJVQH010000383 DAJVQH010000000
VERSION     DAJVQH010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.389"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..401
                     /gene="ydfJ"
                     /locus_tag="OQM87_004387"
     CDS             <1..401
                     /gene="ydfJ"
                     /locus_tag="OQM87_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCS1510230.1"
                     /translation="MLSIIVDKSYAPSTIMVALIVIHNCAVLGLFALENITMAEMFGS
                     KNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMP
                     EVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
BASE COUNT          105 a           94 c           99 g          123 t
ORIGIN      
        1 caatgctttc tatcatcgta gataaaagct atgccccgag caccattatg gttgcactga
       61 ttgtgattca taactgtgcg gtgctgggat tatttgctct ggaaaacatt accatggcag
      121 aaatgttcgg cagtaaaaac cgctttaccc ggatggctat ttcaaaagaa attggtggtc
      181 ttatcgcttc cggttttggt cctatcctgg cgggtatttt ctgcaccatg acggaatcct
      241 ggtatccgat cgccattatg atcatggcat attcagtgat tggtttaatc tctgcgctga
      301 aaatgccaga ggtgaaagac cgtgatttaa gtgcgctgga agacgctgcg gaagatcaac
      361 cgcgtgttgt aagagctgcg caaccttcca gaagtctgta aaccctcaat cccttctctt
      421 a
//
LOCUS       DAJVQH010000384          416 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJVQH010000384 DAJVQH010000000
VERSION     DAJVQH010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.390"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..90)
                     /locus_tag="OQM87_004388"
     CDS             complement(<1..90)
                     /locus_tag="OQM87_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HCS1510231.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
     gene            146..298
                     /locus_tag="OQM87_004389"
                     /pseudo
     CDS             146..298
                     /locus_tag="OQM87_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          122 a           80 c           84 g          130 t
ORIGIN      
        1 aactctagga taaatatttc tcactatata ttcagtattt gcagtattta ttggaaatac
       61 gttcttaata ttcttgatgg gactaatcat tttaatctct atactcaaat ataaacatta
      121 ccatgaacaa tataattgtt cctaagtgga atgtctccac ggagaacact ttatctatcg
      181 ggaaatagtg cgggcaacag tgtttaatta tatcgagtgc gattacaatc gctggcgacg
      241 gcaccgttgg tgtggcggcc tcagtccgga acaatttgaa aaccagaacc tcgcttaggc
      301 ctgtgtccat attacgtggg taggctcaca gagcctcact tacgactgcg tccatattat
      361 gtgggtagga tcaataaaga cgttttatat gtgtctgctt gcagccagaa aattta
//
LOCUS       DAJVQH010000385          416 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJVQH010000385 DAJVQH010000000
VERSION     DAJVQH010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.391"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..239
                     /locus_tag="OQM87_004390"
     CDS             <1..239
                     /locus_tag="OQM87_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCS1510232.1"
                     /translation="NNIGENALRSVAVGRKNYLFFGSDNGGESAAIIYSLLVTCKLNG
                     VEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          102 a           94 c          110 g          110 t
ORIGIN      
        1 acaacaacat cggtgaaaac gcgctgcgat cggtggcggt tggaagaaaa aattatctct
       61 tcttcggttc tgacaacggt ggagaaagcg cggcgattat ctacagtctg ctggtcacct
      121 gcaaactgaa cggagtggag ccggaggact ggttgcgcga agtgatcgtg aagctcaatg
      181 actggccgtc gaaccgagtg catgaactgc tgccctggaa cttctcagct gtaaaataat
      241 ccttccgcta cgtacttctc tgggcgctta ccactcaacg gcatcgtcaa aattatcaaa
      301 gcgcagaaca tagatttatc atgtgtttta tggatggctt tctccatcag gcatcgttcg
      361 tcacgggaaa ttggtgctat gatcggcatt gatcagtccg gttggtgatt tgtttt
//
LOCUS       DAJVQH010000386          412 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJVQH010000386 DAJVQH010000000
VERSION     DAJVQH010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.392"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..>412)
                     /locus_tag="OQM87_004391"
     CDS             complement(<1..>412)
                     /locus_tag="OQM87_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HCS1510233.1"
                     /translation="ALASAGEAGFLPPHHYDQIFTAHGVIMIFFVAMPFVIGLMNLVV
                     PLQIGARDVAFPFLNNLSFWFTVVGVILVNVSLGVGEFAQTGWLAYPPLSGIEYSPGV
                     GVDYWIWSLQLSGIGTTLTGINFFVTILKMRAPGM"
BASE COUNT          116 a          117 c          111 g           68 t
ORIGIN      
        1 catgcccggt gcgcgcatct tcagaatggt aacgaagaag ttgataccgg taagcgtcgt
       61 acctataccg gatagctgga gactccatat ccagtaatca acaccgactc ccggactgta
      121 ctctattccc gatagcggtg gataggccag ccagccggtc tgcgcaaatt cgcccacgcc
      181 gagagaaacg ttaaccagaa tcacgccaac aacagtaaac cagaagctta agttgttgag
      241 gaacgggaac gcaacgtcac gcgcgccgat ctgcagcgga accaccaggt tcatcagacc
      301 gataacgaaa ggcatcgcca cgaagaagat cataatcacg ccgtgcgcgg tgaagatctg
      361 atcgtagtgg tgaggtggca ggaagcccgc ttcacccgcc gaggcaagag cc
//
LOCUS       DAJVQH010000387          408 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJVQH010000387 DAJVQH010000000
VERSION     DAJVQH010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.393"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(7..225)
                     /locus_tag="OQM87_004392"
     CDS             complement(7..225)
                     /locus_tag="OQM87_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510234.1"
                     /translation="MKAVARANNHPYKSVFADFITGHPSCTVCFWETFHKMYPDSPYE
                     YVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT           90 a           99 c          108 g          111 t
ORIGIN      
        1 tgaaaatcac caccacttcg ggtcatccgc cttcatctcc gcttctgttt cgtataaatc
       61 aaaacgacgg caggtatggc agaacgtgac gtattcgtac ggtgaatccg ggtacatttt
      121 gtggaaggtt tcccagaaac agaccgtgca ggagggatga ccggtgataa agtctgcaaa
      181 tacggactta taagggtgat tattggctct ggcgacggct ttcagaaccc tctttcacca
      241 ttctggtgtg gactttctgg tgctccaggt tgtgtgacat gggaactcat tctggatggt
      301 tactctgaaa gctcatattc tgccacaccc cgatttgcag ccgccaggct gccgtggttc
      361 ttacgccaga agggatttgt aactggtctg gtcggctccg gcgtatca
//
LOCUS       DAJVQH010000388          406 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJVQH010000388 DAJVQH010000000
VERSION     DAJVQH010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.394"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(16..>406)
                     /locus_tag="OQM87_004393"
     CDS             complement(16..>406)
                     /locus_tag="OQM87_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCS1510235.1"
                     /translation="GISTRSTTIRIYMLSTGLATLAGIVFSIYTQAGYALAGVGVELD
                     AIASVVIGGTLLSGGVGTVLGTLFGVAIQGLIQTYINFDGTLSSWWTKIAIGILLFIF
                     IALQRGLTVLWENRQSSPVTRVNIAQR"
BASE COUNT          111 a          122 c           90 g           83 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccacc aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atcccc
//
LOCUS       DAJVQH010000389          405 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJVQH010000389 DAJVQH010000000
VERSION     DAJVQH010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.395"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            150..>405
                     /locus_tag="OQM87_004394"
     CDS             150..>405
                     /locus_tag="OQM87_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCS1510236.1"
                     /translation="MRQTEDKQHQNSEPEEGTPCNQTGEDNCPDCGAVMGDATYQETF
                     DEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNNETGE"
BASE COUNT          145 a           85 c          105 g           70 t
ORIGIN      
        1 acattccgcc caaaaatcct gtcgatgcag acaaattact ggctgcctct cgcggagaat
       61 ttgttgaagg gattagcgac ccgaatgatt cgaaatggat tcccggtcat catatctcct
      121 caaatgaagt caaaaaaacg gaaaatgaag tgcgacagac tgaagataaa cagcaccaga
      181 acagcgaacc ggaggaggga acaccctgca atcagactgg cgaggataac tgccctgact
      241 gtggcgcggt gatgggagac gcaacttatc aggaaacttt tgacgaaaaa aaaccagatg
      301 aagcacagga agaagagccg aagaaaacgg aaaaagctga tgatttgtta ccggagaacg
      361 ctggcagcga tcagcataat gatagcaata atgaaactgg cgaag
//
LOCUS       DAJVQH010000390          403 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJVQH010000390 DAJVQH010000000
VERSION     DAJVQH010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.396"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..194)
                     /locus_tag="OQM87_004395"
     CDS             complement(<1..194)
                     /locus_tag="OQM87_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCS1510237.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKGY"
BASE COUNT           99 a          104 c           78 g          122 t
ORIGIN      
        1 tcatacccct tcttcacctt ctccgcaatc agcttcagtt ccgctttttc cgccgccgca
       61 gcatctgaaa aactttttat ctgactttgc cctttggtgc caacttttcc ccaactgata
      121 tgcaactcgt ttccctgttg ctccaccgtc cagaatttat gtgatttttc gtcctgatag
      181 ataaagtgtc tcattctgca tccctgtaat tttatccagt tgaatgcaga tgctaccagt
      241 atttatacgg gttagagaga gacaaaatcg caagaaaaca actttaggaa tttataaaga
      301 aaaggcgctg tcgatgcagc gccttgaagg gggattattt cacctgatga cccggtttag
      361 caccggcatc cgggcttagc aggaaaatat ctttcccgcc agg
//
LOCUS       DAJVQH010000391          392 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJVQH010000391 DAJVQH010000000
VERSION     DAJVQH010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.397"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
BASE COUNT          130 a           95 c           80 g           87 t
ORIGIN      
        1 tcgttactgg tgagaaggcg gaagaaaaag ccagttcttc gcccctaaat aacagcatga
       61 ttccagtcca tgcaaaatct gatttctagc tcacacatac cgagcgctgt acaaaaaacc
      121 gccagccaag gcagttaaga gtgtgtcccc ggggtttgct tagaatattt tgtaagttgt
      181 ccgaagagat atttacaaca ccagaatgat gaatcatcag tcccctgcca gaggttcact
      241 acgtaacctg accgacaatg caccattctg gtgctgaaaa aagagcactg gaactgcaac
      301 aaaacaacat cacagaacag gagatggtaa ggagtcagac attcccacac aatattgtgt
      361 cagtgcaaat aacaaccttc gtctcaaatc tc
//
LOCUS       DAJVQH010000392          383 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJVQH010000392 DAJVQH010000000
VERSION     DAJVQH010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.398"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            162..>383
                     /locus_tag="OQM87_004396"
     CDS             162..>383
                     /locus_tag="OQM87_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1510238.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNA"
BASE COUNT          129 a           84 c           51 g          119 t
ORIGIN      
        1 gcatcgttat atccgtgatg agcgattttt gatattaata tctttcacca tccgctattt
       61 tcaccacttc agaaaacaaa cttattttta ttaaaatcaa ttctttttct ttgaacattc
      121 aactgcgata tttgtaaaaa cattaaacag gcaacaataa aatgacatta ccaacaacta
      181 tttattcatt ccccgcatat ttaccccgtt tttcgtccac cgacaaacca gcaaaattaa
      241 aatttcatca atacgcacgc gctacattac tgtcaaatcg tggaagagat cataattgcg
      301 atggcagacg tactgttgag atccacaaac tagatctctc tgactggcaa gcattcaata
      361 agttagcaac gaagtgtaat gct
//
LOCUS       DAJVQH010000393          381 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJVQH010000393 DAJVQH010000000
VERSION     DAJVQH010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.399"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..>381)
                     /locus_tag="OQM87_004397"
     CDS             complement(<1..>381)
                     /locus_tag="OQM87_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1510239.1"
                     /translation="LDVDNNQLTMLPELPALLEYINADNNQLTMLPELPTSLEVLSVR
                     NNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENRITHIPENI
                     LSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT           99 a           69 c          101 g          112 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag a
//
LOCUS       DAJVQH010000394          380 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJVQH010000394 DAJVQH010000000
VERSION     DAJVQH010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.400"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            107..>380
                     /locus_tag="OQM87_004398"
     CDS             107..>380
                     /locus_tag="OQM87_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCS1510240.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLDHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT           97 a           73 c          105 g          105 t
ORIGIN      
        1 gggcagcgaa ctggataacg tcatggaatt tgaagatatc gccgataacg cagcggatgc
       61 accgactgaa acctgcttca tgcactatga ataagggaga taaatgatgt tacgtttctc
      121 tgctaattta tcgatgttat ttggtgaata tgattttctc gcccgttttg agaaagctgc
      181 gcagtgtggt tttcgcggcg ttgaatttat gtttccttat gactacgaca ttgaagaatt
      241 aaaacaggta ctggcgagta ataaactcga tcatacgctg cacaatttac cggcaggtga
      301 ctgggcggcg ggcgagcgcg gtattgcctg tattcctggc cgtgaagaag agtttcggga
      361 tggcgtagca gcagcgattc
//
LOCUS       DAJVQH010000395          375 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJVQH010000395 DAJVQH010000000
VERSION     DAJVQH010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.401"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>375
                     /locus_tag="OQM87_004399"
     CDS             <1..>375
                     /locus_tag="OQM87_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1510241.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDREALHWAVTT"
BASE COUNT           86 a           96 c          111 g           82 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggatca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aacccgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgtg aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DAJVQH010000396          374 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJVQH010000396 DAJVQH010000000
VERSION     DAJVQH010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.402"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            1..374
                     /locus_tag="OQM87_004400"
                     /pseudo
     CDS             1..374
                     /locus_tag="OQM87_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558297.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           94 a           98 c           98 g           84 t
ORIGIN      
        1 attcttccac taataaagaa cagtcagatg ctcaggccga gatccgccgt ctccagaaag
       61 agctgaaacg ggttaccgac gaacgggaca tattaaaaaa agccgcggcg tacttcgcaa
      121 agctgtccga ctgaggtacg cctttatccg tgacaactcc tgttgctggc ctgttcgcct
      181 gctctgtcgg gtgctggatg ttcatcccag tggtttttac gcctggcttc agcagccgca
      241 ttcacaacgc catcaggcag acctgagact gacaggacag attaaacagt tctggctgga
      301 atcgggatgc gtctatggtt atcgcaaaat ccatctggat ctgcgtgaca gcgggcaaca
      361 gtgcggagta aaca
//
LOCUS       DAJVQH010000397          366 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJVQH010000397 DAJVQH010000000
VERSION     DAJVQH010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.403"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..248
                     /locus_tag="OQM87_004401"
     CDS             <1..248
                     /locus_tag="OQM87_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510242.1"
                     /translation="KFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFLGSI
                     PWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(250..>366)
                     /gene="ruvC"
                     /locus_tag="OQM87_004402"
     CDS             complement(250..>366)
                     /gene="ruvC"
                     /locus_tag="OQM87_004402"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="endonuclease; resolves Holliday structures; forms a
                     complex of RuvABC; the junction binding protein RuvA forms
                     a hexameric ring along with the RuvB helicase and
                     catalyzes branch migration; RuvC then interacts with RuvAB
                     to resolve the Holliday junction by nicking DNA strands of
                     like polarity; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCS1510243.1"
                     /translation="PQADAADALAIAITHCHVSQNAMQMSESRLNLARGRLR"
BASE COUNT           85 a           87 c           99 g           95 t
ORIGIN      
        1 caaaatttgt ctttgatatt tataaagagg cgctatgtat tccggtgggt gaaatagaga
       61 cgtttggaca actgttaaat agcaatccga atgcaaaact cactttctgg aaattctggt
      121 tcttaggttc tattccgtgg gagcgtaaaa ccgtcacgcc agccagtttg tggcatcatc
      181 cgggtttggt gttgattcac gcggagggag ttgaaacgcc tcagccagaa ctgaccgagg
      241 cggtataact taacgcagtc gccctctcgc caggttcagc cgcgattcgc tcatctgcat
      301 cgcattctga ctaacgtggc agtgggtgat ggcaatcgcc agcgcatcgg cggcatccgc
      361 ctgtgg
//
LOCUS       DAJVQH010000398          366 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJVQH010000398 DAJVQH010000000
VERSION     DAJVQH010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.404"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>366
                     /locus_tag="OQM87_004403"
     CDS             <1..>366
                     /locus_tag="OQM87_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510244.1"
                     /translation="FISFDGKTFVYYLYPVTHNRSLIFLLGLERFSLLSKSLAMDSEN
                     LMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVFAYKKDVYLRVCTLII
                     FFAALVAVISGASCLYLVR"
BASE COUNT           84 a           75 c           84 g          123 t
ORIGIN      
        1 gtttatctct tttgacggta aaaccttcgt ctattacctc tatccggtaa ctcacaatcg
       61 tagtctgata tttttgcttg gtctggagcg tttttcttta ctgtcaaaat cgctggcgat
      121 ggacagcgag aacctgatgt tctctctatt taagaacggt aaaccggtga ccggagatga
      181 atataatgct aaaaacgcca tcttcaccgt ttcggaagcg atggagcact tcgcctattt
      241 gccgaccgga ttgtacgtat ttgcgtataa aaaagatgtt tatttgcggg tttgtacatt
      301 gattattttc tttgccgcat tggtggcagt gatatcgggt gccagttgcc tctatctggt
      361 acgcag
//
LOCUS       DAJVQH010000399          365 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJVQH010000399 DAJVQH010000000
VERSION     DAJVQH010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.405"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..314
                     /locus_tag="OQM87_004404"
     CDS             <1..314
                     /locus_tag="OQM87_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1510245.1"
                     /translation="KVERMVQYTRNSFYIPLMTRLRPMGSTVDVETASRHGLRWLHDV
                     ANQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFDNPVTLFVPLIMG
                     C"
BASE COUNT           86 a          104 c           98 g           77 t
ORIGIN      
        1 gtaaggtgga acggatggtg cagtacaccc gtaacagttt ttacatccca ctaatgactc
       61 gcctgcgccc gatggggagc actgtcgatg ttgaaacagc cagccgccac ggtctgcgct
      121 ggctgcacga tgtcgctaac caacgaaagc atgaaacaat ccaggcccgt ccctgtgatc
      181 gctggctcga agagcagcag tccatgctgg cactgcctcc ggagaaaaaa gagtatgacg
      241 tgcatcctgg tgaaaatctg gtgagcttcg acaaccccgt tactttattc gtacccctta
      301 taatggggtg ttagccagcc agaccctgca tgattactgc ccccagtcgt ccatgatccg
      361 ggggg
//
LOCUS       DAJVQH010000400          363 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJVQH010000400 DAJVQH010000000
VERSION     DAJVQH010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.406"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..>363)
                     /locus_tag="OQM87_004405"
     CDS             complement(<1..>363)
                     /locus_tag="OQM87_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCS1510246.1"
                     /translation="DFLHQIKIGEWIHADFKRVRNYAFHKRFFKLLQLGFDYWTPVGG
                     AITPRERKLVSGFVDYLCESVGREHTPALSDAAEQYLNTVATCRTRDTALLKSFDAFR
                     EWVTIQAGFYTEHIYPDG"
BASE COUNT           93 a           92 c           86 g           92 t
ORIGIN      
        1 ctaccatcag gataaatatg ctcggtgtaa aatccggcct gaatggttac ccactcgcgg
       61 aaagcgtcaa acgactttag caatgccgta tcccgggttc tgcatgtcgc aactgtatta
      121 aggtattgct ctgcggcatc actcagggct ggagtgtgtt cccgacctac tgattcgcac
      181 aggtaatcaa cgaagcctga taccagtttt cgttcgcgag gcgtgatcgc cccaccgacc
      241 ggagtccagt aatcaaatcc cagttgcagg agtttgaaaa aacgcttgtg gaatgcgtag
      301 ttacgcacac gcttaaagtc tgcgtgtatc cactcaccta ttttgatttg atgcaaaaaa
      361 tcg
//
LOCUS       DAJVQH010000401          359 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJVQH010000401 DAJVQH010000000
VERSION     DAJVQH010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.407"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..>359)
                     /locus_tag="OQM87_004406"
     CDS             complement(<1..>359)
                     /locus_tag="OQM87_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510247.1"
                     /translation="NIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVTA
                     PSGTEITVEPIEQAILVSHQTKLWDQKSSTGFTRGTLQQDALNICDNVVRTINSKMVD
                     VLESSKLLKTVELPALTG"
BASE COUNT           89 a           91 c          105 g           74 t
ORIGIN      
        1 ccagtcaggg ccggaagctc tacggtcttc agcagcttag aggactccag aacgtcaacc
       61 atcttagagt taatggtacg gaccacgtta tcgcagatgt tcagagcatc ctgctggagc
      121 gtcccccgag tgaaaccagt agaggacttc tggtcccaga gctttgtctg atgagacacg
      181 aggatagcct gttcaatcgg ctcaacggtg atttcagtac cagacggagc ggtcacgact
      241 tcaggtcgct taccaccacg gacaggaacg accacagagc ggtcagccag agtgtgagca
      301 atgattggcg tcggtaagct gttacgggtc ggcaggactt ctacagggaa cggaatgtt
//
LOCUS       DAJVQH010000402          359 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJVQH010000402 DAJVQH010000000
VERSION     DAJVQH010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.408"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..186)
                     /locus_tag="OQM87_004407"
     CDS             complement(<1..186)
                     /locus_tag="OQM87_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCS1510248.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGS"
BASE COUNT           92 a           75 c           95 g           97 t
ORIGIN      
        1 tgagccgcca acggtcagcg cggctatctc atcgtcatct gttgcgagct ggcttgcagc
       61 ttcaatggca ttgagatcga actggctgat tttggcgtcg gcattgtcga aattcagggt
      121 gtattctgga gtgacaacaa tgtcctgttc ttcaggcacc agcttaaagc aggttattat
      181 tttcatgatt tctcctgtga gtcgcatacg gaaccatctc cgatggaagg taaaaatggc
      241 ctgaaaatag tgtgcgatag cggtgcgggg ggttcgacgg agcaagctgt tttccttgcc
      301 aacaatcttg caaaaaaaat gcgattgcta aaacaaaagt gcaatttgcg gcaatatag
//
LOCUS       DAJVQH010000403          356 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJVQH010000403 DAJVQH010000000
VERSION     DAJVQH010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.409"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..105)
                     /locus_tag="OQM87_004408"
     CDS             complement(<1..105)
                     /locus_tag="OQM87_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Wzb shows phosphatase activity towards the
                     autophosphorylated Wzc protein, which induces colanic acid
                     biosynthesis; catalyzes the phosphorylation of UDP-glucose
                     dehydrogenase, an enzyme involved in colanic acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HCS1510249.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVK"
     gene            complement(93..>356)
                     /locus_tag="OQM87_004409"
     CDS             complement(93..>356)
                     /locus_tag="OQM87_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="required for the translocation of capsular
                     polysaccharide through the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCS1510250.1"
                     /translation="ADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQLEP
                     YDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
BASE COUNT           90 a           90 c           87 g           89 t
ORIGIN      
        1 ttttacgccc ggcagacgtt tacgcagcaa gcgctcgcca atgggcgaac ggcaaatgtt
       61 gccggtacaa accactagga ttgagttaaa ttttagttgg gccatgtttg aatccaccgt
      121 gaagtctctg tcaggtcatg caccccggaa atcgtcggta ccagcagaga aatcacacgg
      181 ttccaacgag ccagcggcgc ggtagtgaca taaacaatgt cgtaaggttc aagctggaat
      241 tccgttccga gtatcattgc cgatgcgtcc ttcgcattca gctgataaat attggcgatt
      301 ttgccattct gcttgttttg cgttgcacga ataacaaaaa tccccgtcgc atcagc
//
LOCUS       DAJVQH010000404          348 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJVQH010000404 DAJVQH010000000
VERSION     DAJVQH010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.410"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
BASE COUNT          107 a           92 c           68 g           81 t
ORIGIN      
        1 ttccctcgcg atattaacgt aatatcttaa ttgtatgcac cgccagaatg cgttcagtgc
       61 gatcacggca gcagacaggt aaaaatagca acaaaccacc cgaaaaaccg ccgcgatcgc
      121 gtatgataaa ttttaaccac atgcatacct gtgcagcaag gcgaatcacg cacggatttc
      181 cagccagaga cacctgaaac gtaaccggct catttaaacc gtctggtctg ttacctccgg
      241 ttccacaaaa ataatgtcca tcattttgaa gtggacacta tcgtatggaa taccggacct
      301 gaatcactga agctttacgc cttcatttcg aagaacattt accacggg
//
LOCUS       DAJVQH010000405          347 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJVQH010000405 DAJVQH010000000
VERSION     DAJVQH010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.411"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>347
                     /gene="fimC"
                     /locus_tag="OQM87_004410"
     CDS             <1..>347
                     /gene="fimC"
                     /locus_tag="OQM87_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCS1510251.1"
                     /translation="NADGVKDGRFIVTPPLFAMKGKKENTLRILDATNNQLPQDRESL
                     FWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPPDQAAEKLRFRRSANSL
                     TLINPTPYYLTVT"
BASE COUNT          108 a           78 c           75 g           86 t
ORIGIN      
        1 aaatgccgat ggtgtaaagg atggtcgttt tatcgtgacg cctcctctgt ttgcgatgaa
       61 gggaaaaaaa gagaatacct tacgtattct tgatgcaaca aataaccaat tgccacagga
      121 ccgggaaagt ttattctgga tgaacgttaa agcgattccg tcaatggata aatcaaaatt
      181 gactgagaat acgctacagc tcgcaattat cagccgcatt aaactgtact atcgcccggc
      241 taaattagcg ttgccacccg atcaggccgc agaaaaatta agatttcgtc gtagcgcgaa
      301 ttctctgacg ctgattaacc cgacacccta ttacctgacg gtaacag
//
LOCUS       DAJVQH010000406          346 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJVQH010000406 DAJVQH010000000
VERSION     DAJVQH010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.412"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            24..100
                     /locus_tag="OQM87_004411"
     tRNA            24..100
                     /locus_tag="OQM87_004411"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="OQM87_004412"
     tRNA            143..218
                     /locus_tag="OQM87_004412"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           98 a           72 c           87 g           89 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg agagttgttc gtgagt
//
LOCUS       DAJVQH010000407          345 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJVQH010000407 DAJVQH010000000
VERSION     DAJVQH010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.413"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            229..336
                     /gene="ldrD"
                     /locus_tag="OQM87_004413"
     CDS             229..336
                     /gene="ldrD"
                     /locus_tag="OQM87_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HCS1510252.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           91 a           81 c          100 g           73 t
ORIGIN      
        1 gagacggatt ccggaacaga aaaacagtcg tagaatcagc gtcaagccgg aaggattccg
       61 gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat tggttgctgg agagagaaaa
      121 cccccgcacg ttgcaggtat gcacctgaca acaccacggg ggccattctt gcatccaaac
      181 aatacaagaa tagccgcgaa acgttgtcat tacaatacag gcggctatat gacgttcgca
      241 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg tattcttgcc
      301 agtatgatcg tgaactggct gaacaagcgg aagtaacgtg ccatg
//
LOCUS       DAJVQH010000408          344 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJVQH010000408 DAJVQH010000000
VERSION     DAJVQH010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.414"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            16..>344
                     /locus_tag="OQM87_004414"
     CDS             16..>344
                     /locus_tag="OQM87_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCS1510253.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMP"
BASE COUNT           94 a           82 c           76 g           92 t
ORIGIN      
        1 aataaggaat acactatgac tgttcaaaca agtaaaaacc cgcaggtcga tattgctgaa
       61 gataatgcgt tcttcccttc agaatattcg cttagccaat atacgagtcc cgtctctgat
      121 cttgatggcg tggactatcc aaaaccgtat cgcggtaaac ataaaattct ggtgatcgcc
      181 gcggacgaac gttatttgcc gaccgataac ggaaaactgt tctcgaccgg taaccatccg
      241 attgaaacgt tgctgccgtt gtatcatctc catgctgcag gtttcgaatt cgaagtggcg
      301 accatttccg gtctgatgac caagtttgaa tactgggcta tgcc
//
LOCUS       DAJVQH010000409          344 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJVQH010000409 DAJVQH010000000
VERSION     DAJVQH010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.415"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(89..>344)
                     /locus_tag="OQM87_004415"
     CDS             complement(89..>344)
                     /locus_tag="OQM87_004415"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCS1510254.1"
                     /translation="VRKYFPDATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFY
                     RLGKELALQSGLAHKGDVVVMVSGALVPSGTTNTASVHVL"
BASE COUNT           93 a           79 c           79 g           93 t
ORIGIN      
        1 tgatttttta ttgcttctgg ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg
       61 atatacaaat taattcacaa aagcaatatt acaggacgtg aacagatgcg gtgttagtag
      121 tgccgctcgg taccagtgca ccagaaacca taactacgac gtcacctttg tgtgccagac
      181 cgctctgcag agccagttct ttacccagac ggtagaaatc atcagtagaa gtgatctctt
      241 taaccagctg cggcacaacg cctttgctca gtaccaactg atgagccgtt ttttcgttgg
      301 tggtcagtgc caggatggtg gcatccggga agtatttacg tact
//
LOCUS       DAJVQH010000410          343 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJVQH010000410 DAJVQH010000000
VERSION     DAJVQH010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.416"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..193)
                     /locus_tag="OQM87_004416"
     CDS             complement(<1..193)
                     /locus_tag="OQM87_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510255.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQ"
     gene            complement(144..>343)
                     /locus_tag="OQM87_004417"
     CDS             complement(144..>343)
                     /locus_tag="OQM87_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1510256.1"
                     /translation="SYRGSRLGPVGISVPSTPTGTYIASPQFFITGCSEHSLPGSYCA
                     LSGVPDAHVSGAMPGLFIRTS"
BASE COUNT           81 a           89 c           92 g           81 t
ORIGIN      
        1 gctgacccgt cggtgcgaca tcatcagcgg tggctgtgta ggggactgcc tggtccctgt
       61 cgtcaaaaaa aacgtcacag tctactgcgc cactttcggt ataacgggga ttaatgattt
      121 ttttaatttc cacggtgcat tcctcacgat gtgcgaataa aaagcccggg cattgcgcca
      181 gagacatgtg catccggcac cccggacagg gcgcaatatg accccggtaa tgaatgctct
      241 gaacatcccg taatgaaaaa ttgtggggat gctatatacg ttccggtggg agtactgggc
      301 actgaaatcc ccaccggtcc cagtcgtgag cctctgtatg act
//
LOCUS       DAJVQH010000411          342 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJVQH010000411 DAJVQH010000000
VERSION     DAJVQH010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.417"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            14..>342
                     /locus_tag="OQM87_004418"
     CDS             14..>342
                     /locus_tag="OQM87_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1510257.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACD"
BASE COUNT          104 a           82 c           55 g          101 t
ORIGIN      
        1 cggctttaac tgaatgtcca tcatgttacc gataaataat aacttttcat tgtcccaaaa
       61 ttctttttat aacactattt ccggtacata tgctgattac ttttcagcat gggataaatg
      121 ggaaaaacaa gcgctccccg gtgaaaatcg gaatgaagcg gtctccctac ttaaagaatg
      181 tctcatcaat cagttcagtg agcttcaact gaatcgttta aatctgtcct cgctacctga
      241 caacttacca cctcaaatca ctgttctgga aattactcag aatgccctaa tatcattacc
      301 agaattgcca gcatcgctgg aataccttga cgcctgtgac aa
//
LOCUS       DAJVQH010000412          339 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJVQH010000412 DAJVQH010000000
VERSION     DAJVQH010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.418"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..244)
                     /locus_tag="OQM87_004419"
     CDS             complement(<1..244)
                     /locus_tag="OQM87_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1510258.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKA"
BASE COUNT          103 a           77 c           57 g          102 t
ORIGIN      
        1 atgcctttct aagcatgtaa atctgcccat aataactgct gtcgcttacc gaacctttct
       61 gctcccgcca aacctcattg ataatatgtt gcttatcgga ttctccctgt agtaaaagga
      121 gtaaaaatcg atagccgttt tcagacagat tgattacatt gcctgtactg gtggtgatac
      181 gtcgagacgc tgggtaaaac ttaactccct gccctaaaga aaaataaaaa cggtcatcgc
      241 tcataagtac cgaaccctgc tttcttcttc acatgactac tcattacaac tttgtatgaa
      301 gtaagatgca aaatcatatt gtttacatta agttaagca
//
LOCUS       DAJVQH010000413          339 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJVQH010000413 DAJVQH010000000
VERSION     DAJVQH010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.419"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>339
                     /locus_tag="OQM87_004420"
     CDS             <1..>339
                     /locus_tag="OQM87_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCS1510259.1"
                     /translation="RIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGTLI
                     NYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFALWLLALGA
                     LLLLLCMPLN"
BASE COUNT           78 a           68 c           79 g          114 t
ORIGIN      
        1 gcgtatattc tggcagatag tgtttcctgc aacgctgttt ctgatacttt tccagtttgc
       61 tgcatttgta ggattactgg cgagcagaga aaatctggtc ggtgtcatgc cttttaacct
      121 cgggacttta atcaattatc aggccttact ggtgggtaat ctgatcggtg tcccgctgtg
      181 ctacttcatc attcgggtag tgcgaaatcc attttattta cgtagctatt attcgcaatt
      241 aaaacagcag gttgatgcca aagtcaccaa aaaagagttc gcgctctggc tactggcatt
      301 aggtgcttta ttgttgctgt tatgcatgcc gttaaatga
//
LOCUS       DAJVQH010000414          338 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJVQH010000414 DAJVQH010000000
VERSION     DAJVQH010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.420"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..279
                     /locus_tag="OQM87_004421"
     CDS             <1..279
                     /locus_tag="OQM87_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1510260.1"
                     /translation="TYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDA
                     CIASADNKTIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          113 a           72 c           67 g           86 t
ORIGIN      
        1 acctataaag cgacattacc aatcattgga cgtctgatga acatcagtca ggctatgatt
       61 ataccacttt tcccggttta tgatgaaaaa aaacatctac tgactattga aattcggcca
      121 ccaatggatg cttgcattgc cagcgcggac aataaaacga ttgcccgaca aatgaacaaa
      181 acagtggaaa ttttggtggg gccacatccg gaacagtatg tctgggtttt aaaattgtta
      241 aaaacacgca aatcaaacga agcggacccg tacccttgaa attataaagt acaagtattt
      301 ccactagttg tttgtaagcg tacagcctga accgtctg
//
LOCUS       DAJVQH010000415          336 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJVQH010000415 DAJVQH010000000
VERSION     DAJVQH010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.421"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
BASE COUNT           83 a           68 c           57 g          128 t
ORIGIN      
        1 ctggcgtcgt gcttggtggt ttgctggatt atctggcgct acgaaaaacg catctgacgt
       61 aatctgtaaa tattatttag ctgtagtaat cactcgccgt aattattacg gctaaataaa
      121 catcagcatc gcttattaat atttgttctt ttgtgcggct tagcgtttgg aaattattgg
      181 cgccatttat aaattctatt tttcttacac gattcagcta atgagtcttt attttctcac
      241 cacccagttg tcactctaat gataattatt tgttaaataa ttgttttatt tcgcattggt
      301 tataccaatt gcccgccaag actccttctg cactcc
//
LOCUS       DAJVQH010000416          336 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJVQH010000416 DAJVQH010000000
VERSION     DAJVQH010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.422"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>336
                     /locus_tag="OQM87_004422"
     rRNA            <1..>336
                     /locus_tag="OQM87_004422"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           86 a           76 c          111 g           63 t
ORIGIN      
        1 gtaatgtctg ggaaactgcc tgatggaggg ggataactac tggaaacggt agctaatacc
       61 gcataacgtc gcaagaccaa agagggggac cttcgggcct cttgccatcg gatgtgccca
      121 gatgggatta gctagtaggt ggggtaacgg ctcacctagg cgacgatccc tagctggtct
      181 gagaggatga ccagccacac tggaactgag acacggtcca gactcctacg ggaggcagca
      241 gtggggaata ttgcacaatg ggcgcaagcc tgatgcagcc atgccgcgtg tatgaagaag
      301 gccttcgggt tgtaaagtac tttcagcggg gaggaa
//
LOCUS       DAJVQH010000417          335 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJVQH010000417 DAJVQH010000000
VERSION     DAJVQH010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.423"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>335
                     /locus_tag="OQM87_004423"
     CDS             <1..>335
                     /locus_tag="OQM87_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCS1510261.1"
                     /translation="VGYRSPRARKGEASIVSPNRLQRQFNPDAPDERWVTDITYIRTH
                     EGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPEKQVLVHSDQGSQY
                     TSHEWQSFL"
BASE COUNT           80 a           87 c           95 g           73 t
ORIGIN      
        1 aggtcggata ccgaagcccg cgggcacgta aaggcgaggc cagtatcgtg tcacccaaca
       61 ggctccagcg acagttcaat ccggatgctc ctgatgagcg ttgggtaacg gacataacct
      121 acatcaggac ccacgaaggc tggctgtatc ttgccgttgt tgttgatctg ttctcacgca
      181 aaattatcgg ctggtccatg caatcccgga tgacaaagga cattgtcctg aacgcactgc
      241 tgatggctgt atggcggcgt aatcccgaaa aacaggtgct ggttcattcg gatcagggca
      301 gtcagtacac aagccatgag tggcagtcgt tcctg
//
LOCUS       DAJVQH010000418          333 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJVQH010000418 DAJVQH010000000
VERSION     DAJVQH010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.424"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>333
                     /locus_tag="OQM87_004424"
     CDS             <1..>333
                     /locus_tag="OQM87_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCS1510262.1"
                     /translation="KTGNMFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREM
                     INGKQKTWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLND
                     AHPEWCFRT"
BASE COUNT          100 a           60 c          102 g           71 t
ORIGIN      
        1 aagacgggaa atatgtttaa ccagttgctg gcatccaaaa aattgacgaa aacagccgtc
       61 aatgaagccc tgcgcaggat gaaaaaagcg ggaatagaga aacctgagct ggaagcgttt
      121 ttgcgagaga tgatcaatgg caagcaaaag acctggctgg cgcattgtac tgatgcagag
      181 gcgttatgta ttgatagagt cataagtgag gtgctggcag agcatcctgg attgattagt
      241 gtcctccggc aacggtatga agggcggggg atgactaagc gtaaaatggc tgaattgctg
      301 aatgacgcac accctgagtg gtgtttccgg acg
//
LOCUS       DAJVQH010000419          333 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJVQH010000419 DAJVQH010000000
VERSION     DAJVQH010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.425"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>333
                     /locus_tag="OQM87_004425"
     CDS             <1..>333
                     /locus_tag="OQM87_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1510263.1"
                     /translation="PLQVLGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHP
                     GAFLKIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLLGGLSCLTIPCFA
                     WLADRFGRR"
BASE COUNT           74 a           77 c           86 g           96 t
ORIGIN      
        1 gccacttcag gtactgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt
       61 tgaacaacag caacataatc aagcggccgc gaaaaaacgc atcccggtta tcgaagctct
      121 gttacgacat cccggtgctt tcctgaagat tattgcacta cgactgtgcg agttgctgac
      181 gatgtacatt gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggttaccgcg
      241 cgaacttttc cttaatattg gtttgctgtt aggtggatta agctgcctga caattccctg
      301 ttttgcctgg cttgccgatc gttttggtcg gcg
//
LOCUS       DAJVQH010000420          329 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJVQH010000420 DAJVQH010000000
VERSION     DAJVQH010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.426"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(78..227)
                     /gene="hokB"
                     /locus_tag="OQM87_004426"
     CDS             complement(78..227)
                     /gene="hokB"
                     /locus_tag="OQM87_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCS1510264.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT           81 a           80 c           92 g           76 t
ORIGIN      
        1 aactgaagtg taaaaggggt gccatgagaa aatgaatctg ctagtcagat gcgcggggaa
       61 aatccccgcg cttgccctta cctggacgtg caggccatga gcgcagcaac ctccttatca
      121 ccgtcccgga accgcagttc gtagagcgtt tgtcgggtca ggagtgtgaa tgccagaatc
      181 gtaatgcaga taatgagcag acacaccacc agagggttgc gcttcatagc ctttctcctt
      241 gccggatggc gggtaagagg ctaacatctg aattgcttgg ttcattcgtt ggcctcggtt
      301 tgtactgacc ccaaaaagtt ggacagtta
//
LOCUS       DAJVQH010000421          325 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJVQH010000421 DAJVQH010000000
VERSION     DAJVQH010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.427"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..206
                     /locus_tag="OQM87_004427"
     CDS             <1..206
                     /locus_tag="OQM87_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HCS1510265.1"
                     /translation="WTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGG
                     ELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          104 a           71 c           73 g           77 t
ORIGIN      
        1 aatggacgct tgaagggatc gaaacccgcg ctcagctgct ggattccgat ggtctgctgc
       61 gtcagtcgtc cgatccttat attatggtgc gcgaagcgta cttccagcgt catgatttca
      121 tcgctaatgg cggcgaactc aaaccgcagg aaaatccgaa cgcgcaagcg attcaggatg
      181 atttaaaaga tatcgattct gaataagaaa caaataaaaa aggtgagtcg caaaactcac
      241 cttttttgtt gttatccctc aattaccaca agtaaaagga gataaaccga caatgacccg
      301 gacgtaatga tgtaaaaata ctgat
//
LOCUS       DAJVQH010000422          324 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJVQH010000422 DAJVQH010000000
VERSION     DAJVQH010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.428"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
BASE COUNT           98 a           59 c           67 g          100 t
ORIGIN      
        1 cccagcctgc attgagaatg gctggcataa cgcatttggt aatgatgtct gcttccgtca
       61 ggttacttag gttcagttcc gccatggggt gttctcgctt ttatcaggac gattctgatt
      121 gtacataaaa tcaatagcct ggctatgatt tagggcggtg tttcaacgta aagtacgtag
      181 cagagatcac attgatatct ctgttatatc ttgcttgaat taatctgggc atatcagaga
      241 cagataaaca gaagttacag gaaacatatc aactctaaca aaatatataa aaatcttata
      301 gttgcgtttc tttcgaacaa gaaa
//
LOCUS       DAJVQH010000423          316 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJVQH010000423 DAJVQH010000000
VERSION     DAJVQH010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.429"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..153
                     /locus_tag="OQM87_004428"
     CDS             <1..153
                     /locus_tag="OQM87_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510266.1"
                     /translation="DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLI
                     CRALRK"
BASE COUNT           89 a           65 c           80 g           82 t
ORIGIN      
        1 gatgctgtgg aagagctgcg tcagttgctc gataaccacc atgacctgaa agtcgtggaa
       61 acaccaatct atggttatgt gtggctgaac atggaaacgg cacatcagct tgagttgcta
      121 tcgaatctga tttgccgggc cttgcgcaaa taattcctga acttcagaat catcttgctg
      181 ctgcttcgat tcagcaagga taaagggtat gatagtgaaa agggataaaa gcattgtcat
      241 ctgcggcagc tatgagtaat gttggcccta acgaatagcg gttgcttaaa cgaatccgac
      301 tctcacatta tcaggg
//
LOCUS       DAJVQH010000424          303 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJVQH010000424 DAJVQH010000000
VERSION     DAJVQH010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.430"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>303
                     /locus_tag="OQM87_004429"
     CDS             <1..>303
                     /locus_tag="OQM87_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1510267.1"
                     /translation="SAPVPFVIHLRECSTVVSERVGVAFHGVADGKNPDVLSVGEGPG
                     IASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATGRRVTGGIANAQ"
BASE COUNT           63 a           70 c           91 g           79 t
ORIGIN      
        1 agcgcaccgg tgccttttgt tattcattta cgggaatgta gcacggtggt gagtgaacgt
       61 gtgggtgtgg cgtttcacgg tgtcgcggat ggtaaaaatc cggatgtgct ctccgtggga
      121 gaggggccag ggatagcctc caatattggt gtagcgttgt ttgatgatga aggaaacctc
      181 gtaccgatta atcgtcctcc agcaaactgg aaacggcttt actcaggctc tacttcgcta
      241 catttcatcg ccaaatatcg tgctaccggg cgtcgggtta ctggcggcat cgccaatgcc
      301 cag
//
LOCUS       DAJVQH010000425          302 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJVQH010000425 DAJVQH010000000
VERSION     DAJVQH010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.431"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..270
                     /locus_tag="OQM87_004430"
     CDS             <1..270
                     /locus_tag="OQM87_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCS1510268.1"
                     /translation="EISLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSV
                     DVAISRLRKKLLDNAAEPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
BASE COUNT           93 a           62 c           70 g           77 t
ORIGIN      
        1 gaaatctcgc tctcgacagc tgatttcgaa ttattgtggg aactagctac gcatgccggg
       61 caaatcatgg accgcgatgc attgctgaaa aatttacgcg gcgtcagtta tgacggactg
      121 gatcgtagcg tggacgtggc tatttcgcgg ttaagaaaaa aactgctcga taacgccgca
      181 gaaccttatc gcattaaaac tgtgcgtaac aaaggctatc tttttgcgcc tcatgcatgg
      241 gatagtgaca ataaacaatc agatgactaa tatttgttca tctgaaaaag agaacaatta
      301 ac
//
LOCUS       DAJVQH010000426          295 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJVQH010000426 DAJVQH010000000
VERSION     DAJVQH010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.432"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..>295)
                     /locus_tag="OQM87_004431"
     CDS             complement(<1..>295)
                     /locus_tag="OQM87_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1510269.1"
                     /translation="MDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTK
                     REEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETV"
BASE COUNT           71 a           94 c           55 g           75 t
ORIGIN      
        1 ctcactgtct ccgttatgat gtacaccact tcttccagta accgccgttt cgccggactc
       61 aaaaaaccgt agtacctcac catacggaac cccttatccg ccacatgcca ggagaacctt
      121 tccatgaact catctccact catcaacagg tattcttccc gttttgttcg gtgactgttg
      181 taacgcagac cgatttcatc ctgaccggca taatgctcca gacgactcat cggcaccggt
      241 ggctttttca ggtaagagcc aaagtacacc gccacatggg tggcattatc catca
//
LOCUS       DAJVQH010000427          295 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJVQH010000427 DAJVQH010000000
VERSION     DAJVQH010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.denovo.433"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            <1..>295
                     /locus_tag="OQM87_004432"
     CDS             <1..>295
                     /locus_tag="OQM87_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCS1510270.1"
                     /translation="GFQTALEAYRKVMEIGLKKEASQRAAQFVLGDEGAKRATEFVKN
                     LADSSGVDQIETFSSFAKFSAGAGDMNADQKESLFSNVIGTSRLMGLSTDEING"
BASE COUNT           90 a           43 c           76 g           86 t
ORIGIN      
        1 ggttttcaga cagccttaga agcttatagg aaggttatgg agattggtct gaaaaaagaa
       61 gcatcacaac gagcggcaca attcgtttta ggtgatgaag gtgctaaacg agctacagaa
      121 tttgttaaga atcttgctga tagtagtggt gtagaccaga ttgaaacatt tagcagtttt
      181 gctaagtttt ccgctggtgc tggtgatatg aatgcagacc agaaagaatc cttattttct
      241 aatgtgattg gtacatcccg actaatgggt ttaagcactg atgagattaa cggga
//
LOCUS       DAJVQH010000428         6566 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.guided.169, whole genome shotgun sequence.
ACCESSION   DAJVQH010000428 DAJVQH010000000
VERSION     DAJVQH010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.guided.169"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..443)
                     /gene="epmA"
                     /locus_tag="OQM87_004433"
     CDS             complement(<1..443)
                     /gene="epmA"
                     /locus_tag="OQM87_004433"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCS1510271.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLS"
     gene            768..2576
                     /gene="frdA"
                     /locus_tag="OQM87_004434"
     CDS             768..2576
                     /gene="frdA"
                     /locus_tag="OQM87_004434"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1510272.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            2569..3303
                     /gene="frdB"
                     /locus_tag="OQM87_004435"
     CDS             2569..3303
                     /gene="frdB"
                     /locus_tag="OQM87_004435"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1510273.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            3314..3709
                     /gene="frdC"
                     /locus_tag="OQM87_004436"
     CDS             3314..3709
                     /gene="frdC"
                     /locus_tag="OQM87_004436"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1510274.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3720..4079
                     /gene="frdD"
                     /locus_tag="OQM87_004437"
     CDS             3720..4079
                     /gene="frdD"
                     /locus_tag="OQM87_004437"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1510275.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            4142..5275
                     /locus_tag="OQM87_004438"
     CDS             4142..5275
                     /locus_tag="OQM87_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1510276.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            5364..5897
                     /gene="blc"
                     /locus_tag="OQM87_004439"
     CDS             5364..5897
                     /gene="blc"
                     /locus_tag="OQM87_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1510277.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(5894..6123)
                     /locus_tag="OQM87_004440"
                     /pseudo
     CDS             complement(5894..6123)
                     /locus_tag="OQM87_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(6299..6445)
                     /gene="ecnB"
                     /locus_tag="OQM87_004441"
     CDS             complement(6299..6445)
                     /gene="ecnB"
                     /locus_tag="OQM87_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS1510278.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
BASE COUNT         1634 a         1705 c         1727 g         1500 t
ORIGIN      
        1 gaaaggcttt ctgctgccgg gcagtccagc acctgttgta agagatcgtc cacctcgttc
       61 atcaaccggt acatatcata gtgcggtcga taccactcca gcatagtgaa ctcagggttg
      121 tgataacgcc ccatctcttc attacggaag ctgcggcaca gctggaatac cggcccacaa
      181 ccagcaacca gcaggcgttt catatggtat tccgggctgg tcattaacca gagattcatc
      241 ccctgcgaat gcccggggcc aacgaaacgt gtctcaaacg ggaccaaatg aatatcggtt
      301 accgtcgcct ggctcataca aggcgtctcc acctccagca ctccacgatc ggcaaagaaa
      361 cgacggatct ccgccataat cgccgcgcgt tttaataagt taggaatgga tgcgctcggc
      421 tgccaggatg ccgtttcgct catagttaaa tctccagttt ttgacaaggg cacgaagtct
      481 actcgcaacg cgacggcgag acaaatttta cgcaggaatc aaacagcggt gggcagtgac
      541 taaaaaaagc acgatctgat ggtttagtaa ttaaattaat catcttcagt gataatttag
      601 ccctcttgcg cactaaaaaa atcgatctcg tcaaatttca gacttatcca tcagactata
      661 ctgttgtacc tataaaggag cagtggaata gcgttcgcag accgtaactt tcaggcactt
      721 accctgaagt acggggctgt gggataaaaa caatctggag gaatgtcgtg caaacctttc
      781 aagccgatct tgccattgta ggcgccggtg gcgcgggatt acgtgctgca attgctgccg
      841 cgcaggcaaa tccaaatgca aaaatcgcac taatctcaaa agtatacccg atgcgtagcc
      901 ataccgttgc tgcagaaggg ggctccgccg ctgtcgcgca ggatcatgac agcttcgaat
      961 atcactttca cgatacagta gcgggtggcg actggttgtg tgagcaggat gtcgtggatt
     1021 atttcgtcca ccactgccca accgaaatga cccaactgga actgtgggga tgcccatgga
     1081 gccgtcgccc ggatggtagc gtcaacgtac gtcgcttcgg cggcatgaaa atcgagcgca
     1141 cctggttcgc cgccgataag accggcttcc atatgctgca cacgctgttc cagacctctc
     1201 tgcaattccc gcagatccag cgttttgacg aacatttcgt gctggatatt ctggttgatg
     1261 atggtcatgt tcgcggcctg gtagcaatga acatgatgga aggcacgttg gtgcagatcc
     1321 gtgctaacgc ggtcgttatg gctaccggcg gtgcgggtcg cgtttatcgt tacaacacca
     1381 acggcggcat cgttaccggt gacggtatgg gtatggcgct aagccacggc gttccgctgc
     1441 gtgacatgga attcgttcag tatcacccaa ccggtctgcc aggttccggt atcctgatga
     1501 ccgaaggctg ccgtggtgaa ggcggtattc tggtcaacaa aaatggctac cgttatctgc
     1561 aagattacgg catgggcccg gaaactccgc tgggcgagcc gaaaaacaaa tatatggaac
     1621 tgggtccacg cgacaaagtt tctcaggcct tctggcacga atggcgtaaa ggcaacacca
     1681 tctccacgcc gcgtggtgat gtggtttatc tcgacctgcg tcacctcggc gagaaaaaac
     1741 tgcatgaacg tctgccgttc atctgcgaac tggcgaaagc gtacgttggc gtcgatccgg
     1801 ttaaagaacc gattccggta cgtccgactg cacactacac catgggcggt atcgaaaccg
     1861 atcagaactg tgaaacccgc attaaaggtc tgttcgccgt gggtgaatgt tcctctgttg
     1921 gtctgcacgg tgcaaaccgt ctgggctcca actccctggc ggaactggtg gtcttcggcc
     1981 gtctggccgg tgaacaagcg acagagcgtg cagcaactgc cggtaatggc aacgaagcgg
     2041 caattgaagc gcaggcagct ggcgttgaac aacgtctgaa agatctggtt aaccaggatg
     2101 gcggcgaaaa ctgggcgaag atccgcgacg aaatgggcct ggcaatggaa gaaggttgcg
     2161 gtatctaccg tacgccggaa ctgatgcaga aaaccatcga caagctggca gagctgcagg
     2221 aacgcttcaa gcgcgtgcgc atcaccgaca cctccagcgt gttcaacacc gacctgctct
     2281 acaccattga actgagccac ggtctgaacg ttgctgaatg tatggcgcac tccgcaatgg
     2341 cacgtaaaga gtcccgcggc gcacaccagc gtctggacga aggttgcacc gagcgtgacg
     2401 acgtcaactt tctcaaacac accctcgcct tccgcgatgc tgatggcacg actcgcctgg
     2461 agtacagcga cgtgaagatt actacgctgc cgccagctaa acgcgtttac ggtggcgaag
     2521 cggatgcagc cgataaggcg gaagcagcca ataataagga gaaggcgaat ggctgagatg
     2581 aaaaacctga aaattgaggt ggtgcgctat aacccggaag tcgataccgc accgcatagc
     2641 gcattctatg aagtgcctta tgacgcaact acctcattac tggatgcgct gggctacatc
     2701 aaagacaacc tggcaccgga cctgagttac cgctggtcct gccgtatggc gatttgtggt
     2761 tcctgcggca tgatggttaa caacgtgcca aaactggcat gtaaaacctt cctgcgtgat
     2821 tacaccgacg gtatgaaggt tgaagcgtta gctaacttcc cgattgaacg cgatctggtg
     2881 gtcgatatga cccacttcat cgaaagtctg gaagcgatca aaccgtacat catcggcaac
     2941 tcccgcaccg cggatcaggg tactaacatc cagaccccgg cgcagatggc aaagtatcac
     3001 cagttctccg gttgcatcaa ctgtggtctg tgctacgccg cgtgcccgca gtttggcctg
     3061 aacccagagt tcatcggtcc ggctgccatt acgctggcgc atcgttataa cgaagatagc
     3121 cgcgaccacg gtaagaagga gcgtatggcg cagttgaaca gccagaacgg tgtatggagc
     3181 tgtactttcg tgggctattg ctccgaagtc tgcccgaaac acgtcgatcc ggctgcggcc
     3241 attcagcagg gcaaagtaga aagttcgaaa gactttctta tcgcgaccct gaaaccacgc
     3301 taaggagtgc aacatgacga ctaaacgtaa accgtatgta cggccaatga cgtccacctg
     3361 gtggaaaaaa ttgccgtttt atcgctttta catgctgcgc gaaggcacgg cggttccggc
     3421 tgtgtggttc agcattgaac tgattttcgg gctgtttgcc ctgaaaaatg gcccggaagc
     3481 ctgggcggga ttcgtcgact ttttacaaaa cccggttatc gtgatcatta acctgatcac
     3541 tctgacggca gctctgctgc acaccaaaac ctggtttgaa ctggcaccga aagcggccaa
     3601 tatcattgta aaagacgaaa aaatgggacc agagccaatt atcaaaagtc tctgggcggt
     3661 aactgtggtt gccaccatcg taatcctgtt tgttgccctg tactggtaag gagcctgaga
     3721 tgattaatcc aaatccaaag cgttctgacg aaccggtatt ctggggcctc ttcggggccg
     3781 gtggtatgtg gagcgcaatt attgcgccgg tgatgatcct gctggtgggt attctgctgc
     3841 cactggggct gtttccgggt gatgcgctga gctacgagcg cgttctggcg ttcgcgcaga
     3901 gcttcattgg tcgcgtattc ctgttcctga tgatcgttct gccgctgtgg tgtggtttac
     3961 accgtatgca ccacgcgatg cacgatctga aaatccacgt gcctgcgggc aaatgggttt
     4021 tctacggtct ggctgctatc ctgacagttg tcacgctgat tggtgtcgtt acaatctaac
     4081 gcatcgccaa tgtaaatccg gcccgcctat ggcgggccgt tttgtatgga aaccagacct
     4141 tatgttcaaa acgacgctct gcgccttatt aattaccgcc tcttgctcca catttgctgc
     4201 ccctcaacaa atcaacgata ttgtgcatcg cacaattacc ccgcttatag agcaacaaaa
     4261 gatccccggt atggcggtgg cggtaattta tcagggtaaa ccttattact ttacttgggg
     4321 ctatgcggac atcgccaaaa agcagcccgt cacacagcaa acgttgtttg agttaggttc
     4381 ggtcagcaaa acatttacgg gcgtgcttgg tggcgacgct attgctcgag gggaaatcaa
     4441 gttaagcgat cccgcaacaa aatactggcc tgaacttacc gctaaacagt ggaatgggat
     4501 cacactatta catcttgcga cctacaccgc tggcggcctg ccattgcagg tgccggatga
     4561 agtgaaatcc tcaagcgact tgctgcgctt ctatcaaaac tggcagcctg catgggcccc
     4621 aggaacacaa cgtctgtatg ccaactccag tatcggtttg ttcggcgcac tggctgtgaa
     4681 gccgtctggt ttgagttttg agcaggcgat gcaaactcgt gtcttccagc cactcaaact
     4741 caaccatacg tggattaatg tacctccccc agaagaaaag aattacgcct ggggatatcg
     4801 cgaaggtaag gcagtgcatg tttcgccagg ggcgttagat gctgaagctt atggtgtgaa
     4861 gtcgaccatt gaagatatgg cccgctgggt gcaaagcaat ttaccccccc ttgatatcaa
     4921 tgagaaaacg cttcaacaag ggatacaact ggcacaatct cgctactggc aaaccggcga
     4981 tatgtatcag ggcctgggct gggaaatgct ggactggccg gtcaatcctg acagcatcat
     5041 taacggcagt gacaataaaa ttgcactggc agcacgcccg gtaaaagcga ttaagccccc
     5101 aactcctgca gtacgcgcat catgggtaca taaaacgggg gcgaccggcg gatttggtag
     5161 ctatgtcgcg tttattccag aaaaagagct gggtatcgtg atgctggcta acaaaaacta
     5221 tcccaatcca gagagagtcg ccgccgcctg tcagattctc aacgctctac agtaaaattt
     5281 catcgggtcc gaattttcgg accttttctg cgcttttcct tgctgtcatc tacacttaga
     5341 aaaaaaccag taaggaaaca tttatgcgcc tgctccctct cgttgccgca gcgacagctg
     5401 catttctggt cgttgcctgt agttctccta cgccgccgcg tggcgtgacc gtagtgaata
     5461 atttcgacgc caaacgttat ctcggtacct ggtatgagat tgcccgtttt gatcaccgtt
     5521 ttgaacgtgg actggaaaaa gtcaccgcaa catacagcct gcgtgatgac ggcggcctga
     5581 atgtcattaa taaaggctat aaccctgaca gaggaatgtg gcagcagagt gaagggaaag
     5641 cgtactttac cggcgcacca actcgcgctg cgctgaaagt gtcattcttt ggtcctttct
     5701 atggcggtta taacgttatt gcactcgatc gggaataccg ccatgcgctg gtttgcggcc
     5761 cggaccgcga ctacctgtgg atactctccc gcacgccaac catttctgac gaagtgaaac
     5821 aggagatgct ggcagtcgcg acccgggaag ggtttgatgt cagtaagttt atttgggtac
     5881 agcagcccgg tagttagtga gtgctgagtt tcagaccaat aatccccaat acgattaacg
     5941 ccagactcgc cagacgcatc gggttagcgg actcaccgag cagcacaatg ccggtgatgg
     6001 ccgcgccgac tgcgccaata cccgtccaca cggcatcaaa cggctaaagc cgtgggtata
     6061 tttcaggcca acggcccata ccacttccag cagaccagca ataactaaga taatccagga
     6121 catatcaggc tccggaaatt tggggccgtc cccggtgaaa agaggcgttt gcaggtcgtc
     6181 ctgcaaagct gatggttgaa cggatatttt gccaggtggc aggggaattt caactattac
     6241 ctttatgacg atcgcagaaa aaacaaacgg cacgacactg ctgtcgtacc gttattgctt
     6301 attgctgcgc tttcgttgct gcgccagaaa tcgcgttacc gccatcagaa atgtcttcac
     6361 caacgccacg cgtggtgttg caggcagtta atactgttga aagcaccaga acagaaaaga
     6421 tcgctgcaat tgtcttcttc accataacgt cttcctttta ggtaatgtta tttatgtttg
     6481 cctatagcaa attaataata gacaacatcg ccaaaaatga tggaaatctg atgatttttc
     6541 ggaagagaag aaaaattagc tggcag
//
LOCUS       DAJVQH010000429         5653 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.guided.184, whole genome shotgun sequence.
ACCESSION   DAJVQH010000429 DAJVQH010000000
VERSION     DAJVQH010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.guided.184"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            575..1390
                     /gene="sul2"
                     /locus_tag="OQM87_004442"
     CDS             575..1390
                     /gene="sul2"
                     /locus_tag="OQM87_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1510279.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1477..1779
                     /locus_tag="OQM87_004443"
     CDS             1477..1779
                     /locus_tag="OQM87_004443"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510280.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(1955..3448)
                     /locus_tag="OQM87_004444"
     CDS             complement(1955..3448)
                     /locus_tag="OQM87_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HCS1510281.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(3492..3881)
                     /locus_tag="OQM87_004445"
     CDS             complement(3492..3881)
                     /locus_tag="OQM87_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510282.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4099..4539
                     /locus_tag="OQM87_004446"
     CDS             4099..4539
                     /locus_tag="OQM87_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1510283.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(5273..5422)
                     /locus_tag="OQM87_004447"
     CDS             complement(5273..5422)
                     /locus_tag="OQM87_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1510284.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1355 a         1543 c         1483 g         1272 t
ORIGIN      
        1 taactatcaa cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat
       61 tagtaacagg agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg
      121 gctacactga aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag
      181 cagttcccca actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta
      241 cagagcaaga gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta
      301 tcaattaatc ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa
      361 caggtttccc tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct
      421 acttattgag agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg
      481 atcaccgcaa acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga
      541 ctcatgctcg attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg
      601 catcgtcaac ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc
      661 agccattgcg caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc
      721 ggcatccagc aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc
      781 gccggtgctg gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc
      841 cgcgacgcaa gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt
      901 tccagacgct gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca
      961 ttcggtgcaa gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca
     1021 cattgcggcg ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa
     1081 ccgccttgtc cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct
     1141 ctcggtgctg gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc
     1201 tgtttcgcgc aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc
     1261 cgcgacactc gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca
     1321 cgagccgcgc cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag
     1381 aattcgttaa ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc
     1441 ctttgagggc gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca
     1501 gacggtattc gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc
     1561 ggcatggcgg ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc
     1621 aaggatacgc gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg
     1681 atgggcatgg acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc
     1741 ccttttcacc caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat
     1801 gcaacccaat ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca
     1861 ctagcggctc cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca
     1921 gcattttgat tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct
     1981 cagccggtgg cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct
     2041 tcaggtgatc aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca
     2101 tggcgccgcc gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt
     2161 gcgcccatgt catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga
     2221 ccttgttgcc cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg
     2281 cgaacacccc gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc
     2341 ttgcaatgaa atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct
     2401 tgagctggta gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg
     2461 ggcggctgat gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca
     2521 cgccggcgtg caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac
     2581 tggtcggcag agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac
     2641 ggtaagtgat cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccgagg
     2701 ccagatagct gttttcgaca tcccgttcca gcaggccttg ccgttccaga tagcgaccca
     2761 cccggtgggc gatggtgtgc gtcagctggg tgagctctgg gctggtcggc gccttgaccc
     2821 agcggaaacg cgctgagccg tgggattgct cgacatacac accgtcgaga aacagcatgt
     2881 ggaagtgaac attcagattg agcgccgatc caaaacgctg gatcagggtg accgcgcccg
     2941 tcttggccac ttggtgggta tggcccgctt tcttgaccag gtgcgtggca atgacgcggt
     3001 aaacgatgcc cagcacccac cccatgatct cgggccggct ggcaaacagg aaacgcagct
     3061 gaaacgggaa gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca
     3121 gcaaggcggc actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct
     3181 tacagctgaa agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc
     3241 catgctccag ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca
     3301 attcctttcc ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct
     3361 gatagagaag ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc
     3421 tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata
     3481 cagtctatcg cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga
     3541 cattgccagc cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg
     3601 ttattgcgtg caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa
     3661 ttaatattct tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat
     3721 gcagtataag atgaaccact actattatca tggccaccat taccaccacc gttaaaacta
     3781 cctcccgaac ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga
     3841 ccaaaaccac cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact
     3901 tttaaaaaca gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact
     3961 acgcccgtca aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca
     4021 gcctcagacc ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca
     4081 ggagacactg tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga
     4141 aagtcgcgct cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga
     4201 aaaaatgatc gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc
     4261 acttctgaga caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg
     4321 gaaaatgctc agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc
     4381 ctaatgcttg aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg
     4441 gaacaactac aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg
     4501 ggattctggt cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt
     4561 accataacat ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac
     4621 cggtaaaaca ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta
     4681 cggcatgcat gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg
     4741 taagtcctgt ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg
     4801 cgtggccgtg aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg
     4861 gggtgatgta caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa
     4921 aagtgatgta aaagtgatgt atgaactgaa ggagcgatgt aattctgtag tcaggaagaa
     4981 taacccgttc atgcaggcat gaacgataca gtgaggaacg ctttttttgt cacgatctcc
     5041 ccttaacctg cgtaaatcac ccgccatggt tgattatttt tcaacctcaa aatggtcata
     5101 cacctaacgc agccgcgatt ttcatcgcaa ctgcatgaat taaaagaaaa aaaaggaata
     5161 tttcgttttc acgagtaata cccgggcatt aaaaatccac tgtaattaca tgaaaataaa
     5221 gaaataaaag gtaataccgg agtattacca cgtacgcggg caagcgaaaa agctataacg
     5281 cttcccggcc gacaggcgac ccaaaatcca caggggcatg caggttctct tcagtaacct
     5341 gcgccattaa atcaaaaagg tcatattcgc cgggaagctg cgtatcagtc gaatccagtt
     5401 gttcatcata ttcagatggc atggggtgat atccagtagc tgagaggttg ttagcgggtt
     5461 tcggggcgca gccctgaacc agtcatgtag cgctagcgga gtgtatactg gcttactatg
     5521 ttgccacgat tgcgaaaatc agtgaagtgc ttcatgtggc aggagaaaaa aggcagcacc
     5581 ggcgcgtcag cagaatatgc gatacaggag atattccgct tcctcgctca ctgactcgct
     5641 acgctcggtc gtt
//
LOCUS       DAJVQH010000430         3329 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.guided.230, whole genome shotgun sequence.
ACCESSION   DAJVQH010000430 DAJVQH010000000
VERSION     DAJVQH010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.guided.230"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            273..731
                     /gene="gpt"
                     /locus_tag="OQM87_004448"
     CDS             273..731
                     /gene="gpt"
                     /locus_tag="OQM87_004448"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1510285.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            823..2067
                     /gene="frsA"
                     /locus_tag="OQM87_004449"
     CDS             823..2067
                     /gene="frsA"
                     /locus_tag="OQM87_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1510286.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            2125..2526
                     /gene="crl"
                     /locus_tag="OQM87_004450"
     CDS             2125..2526
                     /gene="crl"
                     /locus_tag="OQM87_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1510287.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(2565..>3329)
                     /locus_tag="OQM87_004451"
     CDS             complement(2565..>3329)
                     /locus_tag="OQM87_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCS1510288.1"
                     /translation="AFAGLKYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTD
                     NFMTKRASGLATYRNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGG
                     SDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPI
                     TGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFN
                     KNMSAFVDYKINKLDSDNKLNINNDDTVAVGMTYQF"
BASE COUNT          816 a          811 c          850 g          852 t
ORIGIN      
        1 agttcagaca cgttaagtct ccttgtcgat cacccgcaaa acagtattgc aggtcacagg
       61 gttagcagaa aatgttgtca acacaagaca ggcttgcgag atatgtattg agaataccac
      121 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
      181 tttttagtgc gccagatatc tataatctcg cgcaacctat tttcccctcg aacacttttt
      241 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
      301 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
      361 aaggcattat tgccgtaagc cgtggcggtc tggtaccggg ggcgttactg gcgcgtgaac
      421 tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg
      481 agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc
      541 tggtggatac cggtggtact gctgttgcga ttcgtgaaat gtatccaaaa gcgcactttg
      601 tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc
      661 cgcaagatac ctggatcgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct
      721 ccggtcgcta atcttttcaa cgcctggcat tgccgggcgt tgttcttttt aacttcaggc
      781 aggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg
      841 agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc
      901 tttaatcacg gcgcacaacc gcctgtgcag tcggctcttg atggtaaaac catccctcac
      961 tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gtattgaccc acgtgaaatc
     1021 ctcgacgtcc aggcacgtat tgtgatgagc gatgctgaac gtaccgacga tgatttatac
     1081 gataccgtga ttggctaccg tggtggcaac tggatttatg agtgggccac ccaggcgatg
     1141 gtgtggcaac aaaaagcctg tacggaagaa gatccgcaac tcagtggtcg acactggctg
     1201 catgcggcta cgttgtacaa cattgccgcc tatcctcatc tgaaaggaga tgacctggcc
     1261 gagcaagcgc aggctttatc aaaccgcgcc tatgaagagg ccgcccagcg tctgccgggc
     1321 acgatgcggc agatggagtt taccgtaccc ggcggtgcgc ccatcaccgg ctttttgcat
     1381 atgccgcaag gcgatggccc gttcccgaca gtattaatgt gtggtggtct ggatgcgatg
     1441 cagacggact attacagcct gtatgaacgt tattttgcgc cgcgcggcat tgcgatgctg
     1501 actattgata tgccgtcggt gggcttttct tcaaaatgga agctcaccca ggactccagc
     1561 ctgttgcatc agcacgtctt aaaggcgctg cctaacgtac cgtgggtgga tcacactcgc
     1621 gtcgcggcct ttggtttccg tttcggcgct aacgttgccg tgcgtctggc ataccttgaa
     1681 tcgccgcgtc tgaaagcggt tgcctgtctt ggtccggtag ttcataccct gttgagtgat
     1741 tttaagtgcc agcaacaggt gccggaaatg tatcttgacg ttctggcgag tcgtttgggg
     1801 atgcatgatg cttccgacga agcgttacgc gtggagctga atcgctattc attaaaagtg
     1861 caaggattgc tggggcgtcg ctgcccaacg ccaatgttat caggctactg gaagaacgat
     1921 ccgttcagcc cggaagagga ctcacgctta atcacctcat catctgctga cggtaaatta
     1981 ttagagatcc catttaaccc ggtgtatcgg aattttgaca aaggtcttca ggaaatcacc
     2041 ggctggatcg aaaaacgctt gtgttaaaaa tttgctaaat tttgccaatt tggtaaaaca
     2101 gttgcatcac aacaggagat agcaatgacg ttaccgagtg gacacccgaa gagcagattg
     2161 atcaaaaaat ttaccgcact aggcccgtat attcgtgaag gtaagtgcga agataatcga
     2221 ttctttttcg attgtctggc tgtatgcgtc aacgtgaaac cggcaccgga agtgcgtgaa
     2281 ttctggggct ggtggatgga gctggaagcg caggaatccc gttttaccta cagctaccag
     2341 tttggtctgt tcgataaagc aggcgactgg aagagtgttc cggtaaaaga tactgaagtg
     2401 gttgaacgac tggagcacac cctgcgtgag tttcacgaga agctgcgtga actgctgacg
     2461 acgctgaatc tgaagctgga accggcggat gattttcgtg atgaaccggt gaagttaacg
     2521 gcgtgagtga aatgtgccgg atgcatcaca tccggcaata ttcattaaaa ctgatacgtc
     2581 atcccaaccg cgacagtatc atcattatta atattcagct tattatcgct atccagtttg
     2641 ttgattttat aatcaacaaa cgctgacata tttttgttga aataatacgt agcaccgacg
     2701 tcgatataat tgaccagatc ttcatcaccg atgccctcaa tatctttccc tttcgataag
     2761 acataaccca gcgatggacg cagaccaaag tcaaactggt attgagcgac cgcttcaaag
     2821 ttctgtgtct tattggcaaa gccgccagtt attggcgtca ttttgcgtgt ttcagaatag
     2881 aaagttgcca gataaatatt attggcatcg tatttcagac ctgttgccca tgcttctgca
     2941 cgcttgcctg tgccacggct ttgcaggttc tgctcgttgg tgcgatctga gttggtatag
     3001 gccccactaa tggcgaaatc gctgccgcca aagtcatatg tcaatgacgt gccgaagcca
     3061 tcgccgtttt gctttttaac gtcgcggttt tcgtttttcc cttgatattg cagggttaag
     3121 ttcaggccat cgataacgcc gaagaagtcg gtgttccgat acgtcgccag accgctggcg
     3181 cgtttggtca taaagttgtc ggtctgcgcc gaggaatcgc caccaaattc cgggaacata
     3241 tcggtccagg cttccacgtc atacaacgcg cccaggttac gaccataatc gaaagaaccc
     3301 aaatctttat atttcaaccc ggcaaaagc
//
LOCUS       DAJVQH010000431         2693 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.guided.241, whole genome shotgun sequence.
ACCESSION   DAJVQH010000431 DAJVQH010000000
VERSION     DAJVQH010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.guided.241"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            22..645
                     /locus_tag="OQM87_004452"
     CDS             22..645
                     /locus_tag="OQM87_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1510289.1"
                     /translation="MSDISRVKILSALMDGRAWTATELSSVANISASTASSHLSKLLD
                     CQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHGVHAKVSTPVHLRKARTCYDHL
                     AGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSER
                     RFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(623..1246)
                     /gene="tetR(B)"
                     /locus_tag="OQM87_004453"
     CDS             complement(623..1246)
                     /gene="tetR(B)"
                     /locus_tag="OQM87_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCS1510290.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1328..2533
                     /gene="tet(B)"
                     /locus_tag="OQM87_004454"
     CDS             1328..2533
                     /gene="tet(B)"
                     /locus_tag="OQM87_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCS1510291.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          698 a          521 c          592 g          882 t
ORIGIN      
        1 atagccgcga taggggcggc tatgtctgac atttcacgag ttaaaatact cagtgctttg
       61 atggatgggc gagcttggac ggccactgag ctaagttctg tggcgaatat atcagcttca
      121 acggcgagca gtcatttatc taaattatta gattgccagc taatcacagt agtagctcaa
      181 ggcaagcatc gttattttcg gctagcagga aaagatattg ctgaattgat ggaaagtatg
      241 atggggatct ccttaaacca tggcgtacat gccaaagttt ccacgccagt gcatttacga
      301 aaagcacgta cttgctatga tcatttagct ggcgaagttg ccgttaagat ctatgattcc
      361 ctttgtcaac agcaatggat cactgaaaat ggttcaatga tcacattaag tggtattcaa
      421 tattttcatg aaatgggaat tgacgttcct tccaaacatt cacgtaaaat ctgttgtgcg
      481 tgtttagatt ggagtgaacg ccgtttccat ttaggtgggt acgttggagc cgcattattt
      541 tcgctttatg aatctaaagg gtggttaact cgacatcttg gttaccgtga agttaccatc
      601 acggaaaaag gttatgctgc ttttaagacc cactttcaca tttaagttgt ttttctaatc
      661 cgcatatgat caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata
      721 attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt
      781 ctttagcgac ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag
      841 cgctgagtgc atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt
      901 gattttcgag agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc
      961 catcgcgatg acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt
     1021 gccagctttc cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg
     1081 ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta
     1141 cacctagctt ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca
     1201 gctctaatgc gctgttaatc actttacttt tatctaatct agacatcatt aattcctaat
     1261 ttttgttgac actctatcat tgatagagtt attttaccac tccctatcag tgatagagaa
     1321 aagtgaaatg aatagttcga caaagatcgc attggtaatt acgttactcg atgccatggg
     1381 gattggcctt atcatgccag tcttgccaac gttattacgt gaatttattg cttcggaaga
     1441 tatcgctaac cactttggcg tattgcttgc actttatgcg ttaatgcagg ttatctttgc
     1501 tccttggctt ggaaaaatgt ctgaccgatt tggtcggcgc ccagtgctgt tgttgtcatt
     1561 aataggcgca tcgctggatt acttattgct ggctttttca agtgcgcttt ggatgctgta
     1621 tttaggccgt ttgctttcag ggatcacagg agctactggg gctgtcgcgg catcggtcat
     1681 tgccgatacc acctcagctt ctcaacgcgt gaagtggttc ggttggttag gggcaagttt
     1741 tgggcttggt ttaatagcgg ggcctattat tggtggtttt gcaggagaga tttcaccgca
     1801 tagtcccttt tttatcgctg cgttgctaaa tattgtcgct ttccttgtgg ttatgttttg
     1861 gttccgtgaa accaaaaata cacgtgataa tacagatacc gaagtagggg ttgagacgca
     1921 atcgaattcg gtatacatca ctttatttaa aacgatgccc attttgttga ttatttattt
     1981 ttcagcgcaa ttgataggcc aaattcccgc aacggtgtgg gtgctattta ccgaaaatcg
     2041 ttttggatgg aatagcatga tggttggctt ttcattagcg ggtcttggtc ttttacactc
     2101 agtattccaa gcctttgtgg caggaagaat agccactaaa tggggcgaaa aaacggcagt
     2161 actgctcgga tttattgcag atagtagtgc atttgccttt ttagcgttta tatctgaagg
     2221 ttggttagtt ttccctgttt taattttatt ggctggtggt gggatcgctt tacctgcatt
     2281 acagggagtg atgtctatcc aaacaaagag tcatcagcaa ggtgctttac agggattatt
     2341 ggtgagcctt accaatgcaa ccggtgttat tggcccatta ctgtttgctg ttatttataa
     2401 tcattcacta ccaatttggg atggctggat ttggattatt ggtttagcgt tttactgtat
     2461 tattatcctg ctatcgatga ccttcatgtt aacccctcaa gctcagggga gtaaacagga
     2521 gacaagtgct tagttatttc gtcaccaaat gatgttattc cgcgaaatat aatgaccctc
     2581 ttgataaccc aagagggcat tttttacgat aaagaagatt tagcttcaaa taaaacctat
     2641 ctattttatt tatctttcaa gctcaataaa aagccgcggt aaatagcaat aaa
//
LOCUS       DAJVQH010000432         2028 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.guided.260, whole genome shotgun sequence.
ACCESSION   DAJVQH010000432 DAJVQH010000000
VERSION     DAJVQH010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.guided.260"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            complement(<1..420)
                     /gene="intI1"
                     /locus_tag="OQM87_004455"
     CDS             complement(<1..420)
                     /gene="intI1"
                     /locus_tag="OQM87_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCS1510292.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLL"
     gene            580..1053
                     /locus_tag="OQM87_004456"
     CDS             580..1053
                     /locus_tag="OQM87_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCS1510293.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1146..1937
                     /gene="aadA1"
                     /locus_tag="OQM87_004457"
     CDS             1146..1937
                     /gene="aadA1"
                     /locus_tag="OQM87_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCS1510294.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          533 a          498 c          538 g          459 t
ORIGIN      
        1 cagaagctgg gcgaacaaac gatgctcgcc ttccagaaaa ccgaggatgc gaaccacttc
       61 atccggggtc agcaccaccg gcaagcgccg cgacggccga ggtcttccga tctcctgaag
      121 ccagggcaga tccgtgcaca gcaccttgcc gtagaagaac agcaaggccg ccaatgcctg
      181 acgatgcgtg gagaccgaaa ccttgcgctc gttcgccagc caggacagaa atgcctcgac
      241 ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac
      301 ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg
      361 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
      421 ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
      481 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
      541 cagtcgccct aaaacaaagt taacctctga ggaagaattg tgaaactatc actaatggta
      601 gctatatcga agaatggagt tatcgggaat ggccctgata ttccatggag tgccaaaggt
      661 gaacagctcc tgtttaaagc tattacctat aaccaatggc tgttggttgg acgcaagact
      721 tttgaatcaa tgggagcatt acccaaccga aagtatgcgg tcgtaacacg ttcaagtttt
      781 acatctgaca atgagaacgt agtgatcttt ccatcaatta aagatgcttt aaccaaccta
      841 aagaaaataa cggatcatgt cattgtttca ggtggtgggg agatatacaa aagcctgatc
      901 gatcaagtag atacactaca tatatctaca atagacatcg agccggaagg tgatgtttac
      961 tttcctgaaa tccccagcaa ttttaggcca gtttttaccc aagacttcgc ctctaacata
     1021 aattatagtt accaaatctg gcaaaagggt taacaagtgg cagcaacgga ttcgcaaacc
     1081 tgtcacgcct tttgtaccaa aagccgcgcc aggtttgcga tccgctgtgc caggcgttaa
     1141 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     1201 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     1261 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     1321 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     1381 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     1441 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     1501 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     1561 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     1621 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     1681 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     1741 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     1801 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     1861 tggcctcgcg cgcagatcag ttggaagaat ttgttcacta cgtgaaaggc gagatcacca
     1921 aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc gcggcgcggc
     1981 ttaactcaag cgttagatgc actaagcaca taattgcggt gatgctgc
//
LOCUS       DAJVQH010000433         1564 bp    DNA     linear   BCT 10-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 504520
            SAMEA8900222-rid15854093.guided.275, whole genome shotgun sequence.
ACCESSION   DAJVQH010000433 DAJVQH010000000
VERSION     DAJVQH010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900222
            Sequence Read Archive: ERR6005074
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477988.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477988.1
            Genome Coverage       :: 32x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 01:59:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,459
            CDSs (total)                      :: 4,382
            Genes (coding)                    :: 4,000
            CDSs (with protein)               :: 4,000
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 196 of 382
            Pseudo Genes (incomplete)         :: 99 of 382
            Pseudo Genes (internal stop)      :: 129 of 382
            Pseudo Genes (multiple problems)  :: 38 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900222-rid15854093.guided.275"
                     /serovar="S. flexneri 3a"
                     /isolate="504520"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-11-25"
     gene            264..923
                     /locus_tag="OQM87_004458"
     CDS             264..923
                     /locus_tag="OQM87_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCS1510295.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="OQM87_004459"
     CDS             complement(1124..1501)
                     /locus_tag="OQM87_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1510296.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
BASE COUNT          423 a          349 c          367 g          425 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagc
//
